smtn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	smtn
Entrez Gene ID	100216161
Full Name	smoothelin
General protein information	Preferred Names smoothelin Names smoothelin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012957509.2	XP_012812963.1	smoothelin isoform X3
XM_018091390.2	XP_017946879.2	smoothelin isoform X2
XM_012957509.3	XP_012812963.2	smoothelin isoform X3
XM_018091387.1	XP_017946876.1	smoothelin isoform X1
XM_018091390.1	XP_017946879.1	smoothelin isoform X2
NM_001142131.2	NP_001135603.2	smoothelin
XM_018091387.2	XP_017946876.2	smoothelin isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	smtn	NP_001135603.2
Rattus norvegicus (Norway rat)	Smtn	NP_001013067.2
Canis lupus familiaris (dog)	SMTN	XP_005636519.1
Homo sapiens (human)	SMTN	NP_008863.3
Pan troglodytes (chimpanzee)	SMTN	XP_003317249.2
	SMTN	XP_002798381.1
Bos taurus (cattle)	SMTN	NP_001070347.1
Mus musculus (house mouse)	Smtn	NP_001271356.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following smtn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smtn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa41717	XM_018091387.1 Latest version!	Xenopus tropicalis smoothelin (smtn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa41718	XM_018091390.1 Latest version!	Xenopus tropicalis smoothelin (smtn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa47669	XM_018091387.2 Latest version!	Xenopus tropicalis smoothelin (smtn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa47670	XM_018091390.2 Latest version!	Xenopus tropicalis smoothelin (smtn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa47671	XM_012957509.3 Latest version!	Xenopus tropicalis smoothelin (smtn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa21468	NM_001142131.2 Latest version!	Xenopus tropicalis smoothelin (smtn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa32649	XM_012957509.2 Latest version!	Xenopus tropicalis smoothelin (smtn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smtn ( XM_018091387.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946876.1 Xenopus tropicalis smoothelin (smtn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa41717
Clone ID Related Accession (Same CDS sequence)	XM_018091387.1
Accession Version	XM_018091387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	smoothelin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GGTGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GACCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG AGAAGAAGCG ATTAAGGAGT 
GATCATCAAA TGGAGGAAAG GGACCAGGAC AGACAGCAAT GCTCAGGTGA AGAGGGCAGT 
GCAGTGAGCA GTGTCCCTGC TGAAGCTCCC CTGTCGGATT CAACATGTGG AGGAGACATC 
GGCGAGGTTG AGGCAGAAAC TCCCCAGGAA ACTCCCCAGC AACTGGAGGT ATTTCTCCCC 
AGCACAACAG AGAAGGCAGA ACCAGAAAAC AAGGGCAAGC TAACAGCAGA GGATCTGAAT 
GCTATAGAGG AAGAGGAAGT TCTGGATAAA ATGCTGGATA AGGCCACAGA CTTTGAGGAG 
AGGAAACTAA TCCGTGCGGC AATGAGGGAT CTGCGTCAGA GAAAGAGAGA GCAACGAGAG 
AAAGAGCGAG AACAGCGCTT ACAGGAGTTA AAGAACAAGG AGAGGGAATC AAAGCTGGCA 
CGAAGCACAG AAACCAGCAT CCGACAGAGC GAGACAACCA ACCATGGCTC TGCCGTCAGC 
ACTGTCACCA AAACACAGAG GCTTGTGCAG TCCAATGATG GCAGCAAAAC CTCTAGCACA 
ACTACATTGG AGGCCAGTTA CATGAAACGA TCAGAGAATG GTGACACCAT TGTTCAGACA 
AAATCATCAT TTAGTGCCAC ATCCAAGAAA GTTGGCAGCA TCTTTGACCG AGAGGATGAG 
GGGCTTTCCA GGGCCAACAG TATGACTGCA ATGGAGCGGC GTCAGGCAGA AAGGAAAAAG 
GAGCTCATGA AGGCTCAAAG CCTACCTAAA ACGCCAGCCA CGCAGGCACG CAAGGCCATG 
ATTGAGAAGA TGGAGAAGGA AAGTGGCGGC TCATCCAGCC CAGCCTTTGC CAAGGTTGCA 
ACACCACGTT CGGCTGCATT TGGTGTGCCT AATGCAAACA GCATAAAGCA AATGCTCTTG 
GATTGGTGCA AAGCCAAGAC GCGTGGGTAT GAGCATGTGA ACATTCAGAA CTTTTCCTCC 
AGCTGGAGCG ATGGAATGGC ATTCTGTGCA CTTGTCCATA ATTTCTTTCC TGAGGCATTT 
GACTACAACC AACTGAACCC CCAGAACAGA AGAAAGAATT TTGACCTGGC CTTTTCAGCT 
GCAGAGATGC TTGTGGACTG TGTCCCTCTG GTGGAGGTGG AGGACATGAT GATCATGGGG 
AAGAGACCAG ACTCCAAGTG TGTCTTCACT TACGTCCAGT CCTTGTACAA CCACCTGCGG 
CGGCATGAGA TGCGTCTACG CAAGTCAGAG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946876.1
CDS	135..3290
Translation	MSGERFSCMDEGSLRKLLEVTLDLAERKEIRSAIRELRRKELERCEEALASKRFRSERSHEQENKENQPRADREEQQQRALNALTIRLQNINDLEELTSLLRNSKEYEERKLIRAAIRKVRNDEIEAASLGGMLIRQSSERDNGKLVCKSQDAVGNLVPSLAQEREDLEERQKIRAQIHELRSAQNGGSHIAGSKEIESNMLLRLKVPTKEQLPSSTSSSSSTSSFTSFSSEPSSSSHCQATELLSLSAEFEGQPHNNRNEVFMEPSAEDSGTETREQPNTHVASHKQNVKSEQKPLPVWTGRADSHIISRLGADNKESFPEGVTSASCSEPDDQTSMPPFRRAYSVRDRVKKFSEETSSVTTPALGFRFGSMRAERAPVRGAHVLQQHSLLSVGQENNQKAAQNARFVTKQEVTRTVSGPCTRRLDGIPTAGTQSTNKIQSFKSPFIPELNNSRSTSLKGSSSLSSTGPCTSSREVNRGLPQSQGSFILKQNSGRSQENQLKVPLPMTERGRIDENSDGANTEGPGSLQSSTHLCKSTKGTREETEDTDMKTLLTIEIKDGRNQTTSSRVVGQPGNQRAELMLGLTSSPFRINSGFSTSNDGASSGITTRSIRTETRTTTQSGITSSSNVIKEKKRLRSDHQMEERDQDRQQCSGEEGSAVSSVPAEAPLSDSTCGGDIGEVEAETPQETPQQLEVFLPSTTEKAEPENKGKLTAEDLNAIEEEEVLDKMLDKATDFEERKLIRAAMRDLRQRKREQREKEREQRLQELKNKERESKLARSTETSIRQSETTNHGSAVSTVTKTQRLVQSNDGSKTSSTTTLEASYMKRSENGDTIVQTKSSFSATSKKVGSIFDREDEGLSRANSMTAMERRQAERKKELMKAQSLPKTPATQARKAMIEKMEKESGGSSSPAFAKVATPRSAAFGVPNANSIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAEMLVDCVPLVEVEDMMIMGKRPDSKCVFTYVQSLYNHLRRHEMRLRKSEF

Target ORF information:

RefSeq Version	XM_018091387.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis smoothelin (smtn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091387.1

ORF Insert Sequence:

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GGTGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GACCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG AGAAGAAGCG ATTAAGGAGT 
GATCATCAAA TGGAGGAAAG GGACCAGGAC AGACAGCAAT GCTCAGGTGA AGAGGGCAGT 
GCAGTGAGCA GTGTCCCTGC TGAAGCTCCC CTGTCGGATT CAACATGTGG AGGAGACATC 
GGCGAGGTTG AGGCAGAAAC TCCCCAGGAA ACTCCCCAGC AACTGGAGGT ATTTCTCCCC 
AGCACAACAG AGAAGGCAGA ACCAGAAAAC AAGGGCAAGC TAACAGCAGA GGATCTGAAT 
GCTATAGAGG AAGAGGAAGT TCTGGATAAA ATGCTGGATA AGGCCACAGA CTTTGAGGAG 
AGGAAACTAA TCCGTGCGGC AATGAGGGAT CTGCGTCAGA GAAAGAGAGA GCAACGAGAG 
AAAGAGCGAG AACAGCGCTT ACAGGAGTTA AAGAACAAGG AGAGGGAATC AAAGCTGGCA 
CGAAGCACAG AAACCAGCAT CCGACAGAGC GAGACAACCA ACCATGGCTC TGCCGTCAGC 
ACTGTCACCA AAACACAGAG GCTTGTGCAG TCCAATGATG GCAGCAAAAC CTCTAGCACA 
ACTACATTGG AGGCCAGTTA CATGAAACGA TCAGAGAATG GTGACACCAT TGTTCAGACA 
AAATCATCAT TTAGTGCCAC ATCCAAGAAA GTTGGCAGCA TCTTTGACCG AGAGGATGAG 
GGGCTTTCCA GGGCCAACAG TATGACTGCA ATGGAGCGGC GTCAGGCAGA AAGGAAAAAG 
GAGCTCATGA AGGCTCAAAG CCTACCTAAA ACGCCAGCCA CGCAGGCACG CAAGGCCATG 
ATTGAGAAGA TGGAGAAGGA AAGTGGCGGC TCATCCAGCC CAGCCTTTGC CAAGGTTGCA 
ACACCACGTT CGGCTGCATT TGGTGTGCCT AATGCAAACA GCATAAAGCA AATGCTCTTG 
GATTGGTGCA AAGCCAAGAC GCGTGGGTAT GAGCATGTGA ACATTCAGAA CTTTTCCTCC 
AGCTGGAGCG ATGGAATGGC ATTCTGTGCA CTTGTCCATA ATTTCTTTCC TGAGGCATTT 
GACTACAACC AACTGAACCC CCAGAACAGA AGAAAGAATT TTGACCTGGC CTTTTCAGCT 
GCAGAGATGC TTGTGGACTG TGTCCCTCTG GTGGAGGTGG AGGACATGAT GATCATGGGG 
AAGAGACCAG ACTCCAAGTG TGTCTTCACT TACGTCCAGT CCTTGTACAA CCACCTGCGG 
CGGCATGAGA TGCGTCTACG CAAGTCAGAG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smtn ( XM_018091390.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946879.1 Xenopus tropicalis smoothelin (smtn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa41718
Clone ID Related Accession (Same CDS sequence)	XM_018091390.1
Accession Version	XM_018091390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	smoothelin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GGTGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GACCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG AGAAGAAGCG ATTAAGGAGT 
GATCATCAAA TGGAGGAAAG GGACCAGGAC AGACAGCAAT GCTCAGGTGA AGAGGGCAGT 
GCAGTGAGCA GTGTCCCTGC TGAAGCTCCC CTGTCGGATT CAACATGTGG AGGAGACATC 
GGCGAGGTTG AGGCAGAAAC TCCCCAGGAA ACTCCCCAGC AACTGGAGGT ATTTCTCCCC 
AGCACAACAG AGAAGGCAGA ACCAGAAAAC AAGGGCAAGC TAACAGCAGA GGATCTGAAT 
GCTATAGAGG AAGAGGAAGT TCTGGATAAA ATGCTGGATA AGGCCACAGA CTTTGAGGAG 
AGGAAACTAA TCCGTGCGGC AATGAGGGAT CTGCGTCAGA GAAAGAGAGA GCAACGAGAG 
AAAGAGCGAG AACAGCGCTT ACAGGAGTTA AAGAACAAGG AGAGGGAATC AAAGCTGGCA 
CGAAGCACAG AAACCAGCAT CCGACAGAGC GAGACAACCA ACCATGGCTC TGCCGTCAGC 
ACTGTCACCA AAACACAGAG GCTTGTGCAG TCCAATGATG GCAGCAAAAC CTCTAGCACA 
ACTACATTGG AGGCCAGTTA CATGAAACGA TCAGAGAATG GTGACACCAT TGTTCAGACA 
AAATCATCAT TTAGTGCCAC ATCCAAGAAA GTTGGCAGCA TCTTTGACCG AGAGGATGAG 
GGGCTTTCCA GGGCCAACAG TATGACTGCA ATGGAGCGGC GTCAGGCAGA AAGGAAAAAG 
GAGCTCATGA AGGCTCAAAG CCTACCTAAA ACGCCAGCCA CGCAGGCACG CAAGGCCATG 
ATTGAGAAGA TGGAGAAGGA AAGTGGCGGC TCATCCAGCC CAGCCTTTGC CAAGGTTGCA 
ACACCACGTT CGGCTGCATT TGGTGTGCCT AATGCAAACA GCATAAAGCA AATGCTCTTG 
GATTGGTGCA AAGCCAAGAC GCGTGGGTAT GAGCATGTGA ACATTCAGAA CTTTTCCTCC 
AGCTGGAGCG ATGGAATGGC ATTCTGTGCA CTTGTCCATA ATTTCTTTCC TGAGGCATTT 
GACTACAACC AACTGAACCC CCAGAACAGA AGAAAGAATT TTGACCTGGC CTTTTCAGCT 
GCAGAGAAAC ACGCAGACTG TCCGCAGTTG CTGGACGCAG AGGATATGGT CCGCATGCGA 
GAGCCGGACT GGAAGTGTGT TTACACGTAC ATCCAGGAGT TCTACCGCTG CCTGGTGCAG 
AAGGGGATGG TAAAAACCAA AAAGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946879.1
CDS	135..3284
Translation	MSGERFSCMDEGSLRKLLEVTLDLAERKEIRSAIRELRRKELERCEEALASKRFRSERSHEQENKENQPRADREEQQQRALNALTIRLQNINDLEELTSLLRNSKEYEERKLIRAAIRKVRNDEIEAASLGGMLIRQSSERDNGKLVCKSQDAVGNLVPSLAQEREDLEERQKIRAQIHELRSAQNGGSHIAGSKEIESNMLLRLKVPTKEQLPSSTSSSSSTSSFTSFSSEPSSSSHCQATELLSLSAEFEGQPHNNRNEVFMEPSAEDSGTETREQPNTHVASHKQNVKSEQKPLPVWTGRADSHIISRLGADNKESFPEGVTSASCSEPDDQTSMPPFRRAYSVRDRVKKFSEETSSVTTPALGFRFGSMRAERAPVRGAHVLQQHSLLSVGQENNQKAAQNARFVTKQEVTRTVSGPCTRRLDGIPTAGTQSTNKIQSFKSPFIPELNNSRSTSLKGSSSLSSTGPCTSSREVNRGLPQSQGSFILKQNSGRSQENQLKVPLPMTERGRIDENSDGANTEGPGSLQSSTHLCKSTKGTREETEDTDMKTLLTIEIKDGRNQTTSSRVVGQPGNQRAELMLGLTSSPFRINSGFSTSNDGASSGITTRSIRTETRTTTQSGITSSSNVIKEKKRLRSDHQMEERDQDRQQCSGEEGSAVSSVPAEAPLSDSTCGGDIGEVEAETPQETPQQLEVFLPSTTEKAEPENKGKLTAEDLNAIEEEEVLDKMLDKATDFEERKLIRAAMRDLRQRKREQREKEREQRLQELKNKERESKLARSTETSIRQSETTNHGSAVSTVTKTQRLVQSNDGSKTSSTTTLEASYMKRSENGDTIVQTKSSFSATSKKVGSIFDREDEGLSRANSMTAMERRQAERKKELMKAQSLPKTPATQARKAMIEKMEKESGGSSSPAFAKVATPRSAAFGVPNANSIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAEKHADCPQLLDAEDMVRMREPDWKCVYTYIQEFYRCLVQKGMVKTKKS

Target ORF information:

RefSeq Version	XM_018091390.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis smoothelin (smtn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091390.1

ORF Insert Sequence:

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GGTGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GACCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG AGAAGAAGCG ATTAAGGAGT 
GATCATCAAA TGGAGGAAAG GGACCAGGAC AGACAGCAAT GCTCAGGTGA AGAGGGCAGT 
GCAGTGAGCA GTGTCCCTGC TGAAGCTCCC CTGTCGGATT CAACATGTGG AGGAGACATC 
GGCGAGGTTG AGGCAGAAAC TCCCCAGGAA ACTCCCCAGC AACTGGAGGT ATTTCTCCCC 
AGCACAACAG AGAAGGCAGA ACCAGAAAAC AAGGGCAAGC TAACAGCAGA GGATCTGAAT 
GCTATAGAGG AAGAGGAAGT TCTGGATAAA ATGCTGGATA AGGCCACAGA CTTTGAGGAG 
AGGAAACTAA TCCGTGCGGC AATGAGGGAT CTGCGTCAGA GAAAGAGAGA GCAACGAGAG 
AAAGAGCGAG AACAGCGCTT ACAGGAGTTA AAGAACAAGG AGAGGGAATC AAAGCTGGCA 
CGAAGCACAG AAACCAGCAT CCGACAGAGC GAGACAACCA ACCATGGCTC TGCCGTCAGC 
ACTGTCACCA AAACACAGAG GCTTGTGCAG TCCAATGATG GCAGCAAAAC CTCTAGCACA 
ACTACATTGG AGGCCAGTTA CATGAAACGA TCAGAGAATG GTGACACCAT TGTTCAGACA 
AAATCATCAT TTAGTGCCAC ATCCAAGAAA GTTGGCAGCA TCTTTGACCG AGAGGATGAG 
GGGCTTTCCA GGGCCAACAG TATGACTGCA ATGGAGCGGC GTCAGGCAGA AAGGAAAAAG 
GAGCTCATGA AGGCTCAAAG CCTACCTAAA ACGCCAGCCA CGCAGGCACG CAAGGCCATG 
ATTGAGAAGA TGGAGAAGGA AAGTGGCGGC TCATCCAGCC CAGCCTTTGC CAAGGTTGCA 
ACACCACGTT CGGCTGCATT TGGTGTGCCT AATGCAAACA GCATAAAGCA AATGCTCTTG 
GATTGGTGCA AAGCCAAGAC GCGTGGGTAT GAGCATGTGA ACATTCAGAA CTTTTCCTCC 
AGCTGGAGCG ATGGAATGGC ATTCTGTGCA CTTGTCCATA ATTTCTTTCC TGAGGCATTT 
GACTACAACC AACTGAACCC CCAGAACAGA AGAAAGAATT TTGACCTGGC CTTTTCAGCT 
GCAGAGAAAC ACGCAGACTG TCCGCAGTTG CTGGACGCAG AGGATATGGT CCGCATGCGA 
GAGCCGGACT GGAAGTGTGT TTACACGTAC ATCCAGGAGT TCTACCGCTG CCTGGTGCAG 
AAGGGGATGG TAAAAACCAA AAAGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smtn ( XM_018091387.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946876.2 Xenopus tropicalis smoothelin (smtn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa47669
Clone ID Related Accession (Same CDS sequence)	XM_018091387.2
Accession Version	XM_018091387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	smoothelin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GATGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GTCCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG AGAAGAAGCG ATTAAGGAGT 
GATCATCAAA TGGAGGAAAG GGACCAGGAC AGACAGCAAT GCTCAGGTGA AGAGGGCAGT 
GCAGTGAGCA GTGTCCCTGC TGAAGCTCCC CTGTCGGATT CAACATGTGG AGGAGACATC 
GGCGAGGTTG AGGCAGAAAC TCCCCAGGAA ACTCCCCAGC AACTGGAGGT ATTTCTCCCC 
AGCACAACAG AGAAGGCAGA ACCAGAAAAC AAGGGCAAGC TAACAGCAGA GGATCTGAAT 
GCTATAGAGG AAGAGGAAGT TCTGGATAAA ATGCTGGATA AGGCCACAGA CTTTGAGGAG 
AGGAAACTAA TCCGTGCGGC AATGAGGGAT CTGCGTCAGA GAAAGAGAGA GCAACGAGAG 
AAAGAGCGAG AACAGCGCTT ACAGGAGTTA AAGAACAAGG AGAGGGAATC TCAGCTGGCA 
CGAAGCACAG AAACCAGCAT CCGACAGAGC GAGACAACCA ACCATGGCTC TGCCGTCAGC 
ACTGTCACCA AAACACAGAG GCTTGTGCAG TCCAATGATG GCAGCAAAAC CTCTAGCACA 
ACTACATTGG AGGCCAGTTA CATGAAACGA TCAGAGAATG GTGACACCAT TGTTCAGACA 
AAATCATCAT TTAGTGCCAC ATCCAAGAAA GTTGGCAGCA TCTTTGACCG AGAGGATGAG 
GGGCTTTCCA GGGCCAACAG TATGACTGCA ATGGAGCGGC GTCAGGCAGA AAGGAAAAAG 
GAGCTCATGA AGGCTCAAAG CCTACCTAAA ACGCCAGCCA CGCAGGCACG CAAGGCCATG 
ATTGAGAAGA TGGAGAAGGA AAGTGGCGGC TCATCCAGCC CAGCCTTTGC CAAGGTTGCA 
ACACCACGTT CGGCTGCATT TGGTGTGCCT AATGCAAACA GCATAAAGCA AATGCTCTTG 
GATTGGTGCA AAGCCAAGAC GCGTGGGTAT GAGCATGTGA ACATTCAGAA CTTTTCCTCC 
AGCTGGAGCG ATGGAATGGC ATTCTGTGCA CTTGTCCATA ATTTCTTTCC TGAGGCATTT 
GACTACAACC AACTGAACCC CCAGAACAGA AGAAAGAATT TTGACCTGGC CTTTTCAGCT 
GCAGAGATGC TTGTGGACTG TGTCCCTCTG GTGGAGGTGG AGGACATGAT GATCATGGGG 
AAGAGACCAG ACTCCAAGTG TGTCTTCACT TACGTCCAGT CCTTGTACAA CCACCTGCGG 
CGGCATGAGA TGCGTCTACG CAAGTCAGAG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946876.2
CDS	128..3283
Translation	MSGERFSCMDEGSLRKLLEVTLDLAERKEIRSAIRELRRKELERCEEALASKRFRSERSHEQENKENQPRADREEQQQRALNALTIRLQNINDLEELTSLLRNSKEYEERKLIRAAIRKVRNDEIEAASLGGMLIRQSSERDNGKLVCKSQDAVGNLVPSLAQEREDLEERQKIRAQIHELRSAQNGGSHIAGSKEIESNMLLRLKVPTKEQLPSSTSSSSSTSSFTSFSSEPSSSSHCQATELLSLSAEFEGQPHNNRNEVFMEPSAEDSGTETREQPNTHVASHKQNVKSEQKPLPVWTGRADSHIISRLGADNKESFPEGVTSASCSEPDDQTSMPPFRRAYSVRDRVKKFSEESSSVTTPALGFRFGSMRAERAPVRGAHVLQQHSLLSVGQENNQKAAQNARFVTKQEVTRTVSGPCTRRLDGIPTAGTQSTNKIQSFKSPFIPELNNSRSTSLKGSSSLSSTGPCTSSREVNRGLPQSQGSFILKQNSGRSQENQLKVPLPMTERGRIDENSDGANTEGPGSLQSSTHLCKSTKGTREETEDTDMKTLLTIEIKDGRNQTTSSRVVGQPGNQRAELMLGLTSSPFRINSGFSTSNDGASSGITTRSIRTETRTTTQSGITSSSNVIKEKKRLRSDHQMEERDQDRQQCSGEEGSAVSSVPAEAPLSDSTCGGDIGEVEAETPQETPQQLEVFLPSTTEKAEPENKGKLTAEDLNAIEEEEVLDKMLDKATDFEERKLIRAAMRDLRQRKREQREKEREQRLQELKNKERESQLARSTETSIRQSETTNHGSAVSTVTKTQRLVQSNDGSKTSSTTTLEASYMKRSENGDTIVQTKSSFSATSKKVGSIFDREDEGLSRANSMTAMERRQAERKKELMKAQSLPKTPATQARKAMIEKMEKESGGSSSPAFAKVATPRSAAFGVPNANSIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAEMLVDCVPLVEVEDMMIMGKRPDSKCVFTYVQSLYNHLRRHEMRLRKSEF

Target ORF information:

RefSeq Version	XM_018091387.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis smoothelin (smtn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091387.2

ORF Insert Sequence:

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GATGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GTCCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG AGAAGAAGCG ATTAAGGAGT 
GATCATCAAA TGGAGGAAAG GGACCAGGAC AGACAGCAAT GCTCAGGTGA AGAGGGCAGT 
GCAGTGAGCA GTGTCCCTGC TGAAGCTCCC CTGTCGGATT CAACATGTGG AGGAGACATC 
GGCGAGGTTG AGGCAGAAAC TCCCCAGGAA ACTCCCCAGC AACTGGAGGT ATTTCTCCCC 
AGCACAACAG AGAAGGCAGA ACCAGAAAAC AAGGGCAAGC TAACAGCAGA GGATCTGAAT 
GCTATAGAGG AAGAGGAAGT TCTGGATAAA ATGCTGGATA AGGCCACAGA CTTTGAGGAG 
AGGAAACTAA TCCGTGCGGC AATGAGGGAT CTGCGTCAGA GAAAGAGAGA GCAACGAGAG 
AAAGAGCGAG AACAGCGCTT ACAGGAGTTA AAGAACAAGG AGAGGGAATC TCAGCTGGCA 
CGAAGCACAG AAACCAGCAT CCGACAGAGC GAGACAACCA ACCATGGCTC TGCCGTCAGC 
ACTGTCACCA AAACACAGAG GCTTGTGCAG TCCAATGATG GCAGCAAAAC CTCTAGCACA 
ACTACATTGG AGGCCAGTTA CATGAAACGA TCAGAGAATG GTGACACCAT TGTTCAGACA 
AAATCATCAT TTAGTGCCAC ATCCAAGAAA GTTGGCAGCA TCTTTGACCG AGAGGATGAG 
GGGCTTTCCA GGGCCAACAG TATGACTGCA ATGGAGCGGC GTCAGGCAGA AAGGAAAAAG 
GAGCTCATGA AGGCTCAAAG CCTACCTAAA ACGCCAGCCA CGCAGGCACG CAAGGCCATG 
ATTGAGAAGA TGGAGAAGGA AAGTGGCGGC TCATCCAGCC CAGCCTTTGC CAAGGTTGCA 
ACACCACGTT CGGCTGCATT TGGTGTGCCT AATGCAAACA GCATAAAGCA AATGCTCTTG 
GATTGGTGCA AAGCCAAGAC GCGTGGGTAT GAGCATGTGA ACATTCAGAA CTTTTCCTCC 
AGCTGGAGCG ATGGAATGGC ATTCTGTGCA CTTGTCCATA ATTTCTTTCC TGAGGCATTT 
GACTACAACC AACTGAACCC CCAGAACAGA AGAAAGAATT TTGACCTGGC CTTTTCAGCT 
GCAGAGATGC TTGTGGACTG TGTCCCTCTG GTGGAGGTGG AGGACATGAT GATCATGGGG 
AAGAGACCAG ACTCCAAGTG TGTCTTCACT TACGTCCAGT CCTTGTACAA CCACCTGCGG 
CGGCATGAGA TGCGTCTACG CAAGTCAGAG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smtn ( XM_018091390.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946879.2 Xenopus tropicalis smoothelin (smtn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa47670
Clone ID Related Accession (Same CDS sequence)	XM_018091390.2
Accession Version	XM_018091390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	smoothelin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GATGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GTCCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG AGAAGAAGCG ATTAAGGAGT 
GATCATCAAA TGGAGGAAAG GGACCAGGAC AGACAGCAAT GCTCAGGTGA AGAGGGCAGT 
GCAGTGAGCA GTGTCCCTGC TGAAGCTCCC CTGTCGGATT CAACATGTGG AGGAGACATC 
GGCGAGGTTG AGGCAGAAAC TCCCCAGGAA ACTCCCCAGC AACTGGAGGT ATTTCTCCCC 
AGCACAACAG AGAAGGCAGA ACCAGAAAAC AAGGGCAAGC TAACAGCAGA GGATCTGAAT 
GCTATAGAGG AAGAGGAAGT TCTGGATAAA ATGCTGGATA AGGCCACAGA CTTTGAGGAG 
AGGAAACTAA TCCGTGCGGC AATGAGGGAT CTGCGTCAGA GAAAGAGAGA GCAACGAGAG 
AAAGAGCGAG AACAGCGCTT ACAGGAGTTA AAGAACAAGG AGAGGGAATC TCAGCTGGCA 
CGAAGCACAG AAACCAGCAT CCGACAGAGC GAGACAACCA ACCATGGCTC TGCCGTCAGC 
ACTGTCACCA AAACACAGAG GCTTGTGCAG TCCAATGATG GCAGCAAAAC CTCTAGCACA 
ACTACATTGG AGGCCAGTTA CATGAAACGA TCAGAGAATG GTGACACCAT TGTTCAGACA 
AAATCATCAT TTAGTGCCAC ATCCAAGAAA GTTGGCAGCA TCTTTGACCG AGAGGATGAG 
GGGCTTTCCA GGGCCAACAG TATGACTGCA ATGGAGCGGC GTCAGGCAGA AAGGAAAAAG 
GAGCTCATGA AGGCTCAAAG CCTACCTAAA ACGCCAGCCA CGCAGGCACG CAAGGCCATG 
ATTGAGAAGA TGGAGAAGGA AAGTGGCGGC TCATCCAGCC CAGCCTTTGC CAAGGTTGCA 
ACACCACGTT CGGCTGCATT TGGTGTGCCT AATGCAAACA GCATAAAGCA AATGCTCTTG 
GATTGGTGCA AAGCCAAGAC GCGTGGGTAT GAGCATGTGA ACATTCAGAA CTTTTCCTCC 
AGCTGGAGCG ATGGAATGGC ATTCTGTGCA CTTGTCCATA ATTTCTTTCC TGAGGCATTT 
GACTACAACC AACTGAACCC CCAGAACAGA AGAAAGAATT TTGACCTGGC CTTTTCAGCT 
GCAGAGAAAC ACGCAGACTG TCCGCAGTTG CTGGACGCAG AGGATATGGT CCGCATGCGA 
GAGCCGGACT GGAAGTGTGT TTACACGTAC ATCCAGGAGT TCTACCGCTG CCTGGTGCAG 
AAGGGGATGG TAAAAACCAA AAAGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946879.2
CDS	128..3277
Translation	MSGERFSCMDEGSLRKLLEVTLDLAERKEIRSAIRELRRKELERCEEALASKRFRSERSHEQENKENQPRADREEQQQRALNALTIRLQNINDLEELTSLLRNSKEYEERKLIRAAIRKVRNDEIEAASLGGMLIRQSSERDNGKLVCKSQDAVGNLVPSLAQEREDLEERQKIRAQIHELRSAQNGGSHIAGSKEIESNMLLRLKVPTKEQLPSSTSSSSSTSSFTSFSSEPSSSSHCQATELLSLSAEFEGQPHNNRNEVFMEPSAEDSGTETREQPNTHVASHKQNVKSEQKPLPVWTGRADSHIISRLGADNKESFPEGVTSASCSEPDDQTSMPPFRRAYSVRDRVKKFSEESSSVTTPALGFRFGSMRAERAPVRGAHVLQQHSLLSVGQENNQKAAQNARFVTKQEVTRTVSGPCTRRLDGIPTAGTQSTNKIQSFKSPFIPELNNSRSTSLKGSSSLSSTGPCTSSREVNRGLPQSQGSFILKQNSGRSQENQLKVPLPMTERGRIDENSDGANTEGPGSLQSSTHLCKSTKGTREETEDTDMKTLLTIEIKDGRNQTTSSRVVGQPGNQRAELMLGLTSSPFRINSGFSTSNDGASSGITTRSIRTETRTTTQSGITSSSNVIKEKKRLRSDHQMEERDQDRQQCSGEEGSAVSSVPAEAPLSDSTCGGDIGEVEAETPQETPQQLEVFLPSTTEKAEPENKGKLTAEDLNAIEEEEVLDKMLDKATDFEERKLIRAAMRDLRQRKREQREKEREQRLQELKNKERESQLARSTETSIRQSETTNHGSAVSTVTKTQRLVQSNDGSKTSSTTTLEASYMKRSENGDTIVQTKSSFSATSKKVGSIFDREDEGLSRANSMTAMERRQAERKKELMKAQSLPKTPATQARKAMIEKMEKESGGSSSPAFAKVATPRSAAFGVPNANSIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAEKHADCPQLLDAEDMVRMREPDWKCVYTYIQEFYRCLVQKGMVKTKKS

Target ORF information:

RefSeq Version	XM_018091390.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis smoothelin (smtn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091390.2

ORF Insert Sequence:

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GATGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GTCCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG AGAAGAAGCG ATTAAGGAGT 
GATCATCAAA TGGAGGAAAG GGACCAGGAC AGACAGCAAT GCTCAGGTGA AGAGGGCAGT 
GCAGTGAGCA GTGTCCCTGC TGAAGCTCCC CTGTCGGATT CAACATGTGG AGGAGACATC 
GGCGAGGTTG AGGCAGAAAC TCCCCAGGAA ACTCCCCAGC AACTGGAGGT ATTTCTCCCC 
AGCACAACAG AGAAGGCAGA ACCAGAAAAC AAGGGCAAGC TAACAGCAGA GGATCTGAAT 
GCTATAGAGG AAGAGGAAGT TCTGGATAAA ATGCTGGATA AGGCCACAGA CTTTGAGGAG 
AGGAAACTAA TCCGTGCGGC AATGAGGGAT CTGCGTCAGA GAAAGAGAGA GCAACGAGAG 
AAAGAGCGAG AACAGCGCTT ACAGGAGTTA AAGAACAAGG AGAGGGAATC TCAGCTGGCA 
CGAAGCACAG AAACCAGCAT CCGACAGAGC GAGACAACCA ACCATGGCTC TGCCGTCAGC 
ACTGTCACCA AAACACAGAG GCTTGTGCAG TCCAATGATG GCAGCAAAAC CTCTAGCACA 
ACTACATTGG AGGCCAGTTA CATGAAACGA TCAGAGAATG GTGACACCAT TGTTCAGACA 
AAATCATCAT TTAGTGCCAC ATCCAAGAAA GTTGGCAGCA TCTTTGACCG AGAGGATGAG 
GGGCTTTCCA GGGCCAACAG TATGACTGCA ATGGAGCGGC GTCAGGCAGA AAGGAAAAAG 
GAGCTCATGA AGGCTCAAAG CCTACCTAAA ACGCCAGCCA CGCAGGCACG CAAGGCCATG 
ATTGAGAAGA TGGAGAAGGA AAGTGGCGGC TCATCCAGCC CAGCCTTTGC CAAGGTTGCA 
ACACCACGTT CGGCTGCATT TGGTGTGCCT AATGCAAACA GCATAAAGCA AATGCTCTTG 
GATTGGTGCA AAGCCAAGAC GCGTGGGTAT GAGCATGTGA ACATTCAGAA CTTTTCCTCC 
AGCTGGAGCG ATGGAATGGC ATTCTGTGCA CTTGTCCATA ATTTCTTTCC TGAGGCATTT 
GACTACAACC AACTGAACCC CCAGAACAGA AGAAAGAATT TTGACCTGGC CTTTTCAGCT 
GCAGAGAAAC ACGCAGACTG TCCGCAGTTG CTGGACGCAG AGGATATGGT CCGCATGCGA 
GAGCCGGACT GGAAGTGTGT TTACACGTAC ATCCAGGAGT TCTACCGCTG CCTGGTGCAG 
AAGGGGATGG TAAAAACCAA AAAGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smtn ( XM_012957509.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812963.2 Xenopus tropicalis smoothelin (smtn), transcript variant X3, mRNA.

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CloneID	OXa47671
Clone ID Related Accession (Same CDS sequence)	XM_012957509.3
Accession Version	XM_012957509.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	smoothelin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957509.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GATGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GTCCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG TTGAGGCAGA AACTCCCCAG 
GAAACTCCCC AGCAACTGGA GGTATTTCTC CCCAGCACAA CAGAGAAGGC AGAACCAGAA 
AACAAGGGCA AGCTAACAGC AGAGGATCTG AATGCTATAG AGGAAGAGGA AGTTCTGGAT 
AAAATGCTGG ATAAGGCCAC AGACTTTGAG GAGAGGAAAC TAATCCGTGC GGCAATGAGG 
GATCTGCGTC AGAGAAAGAG AGAGCAACGA GAGAAAGAGC GAGAACAGCG CTTACAGGAG 
TTAAAGAACA AGGAGAGGGA ATCTCAGCTG GCACGAAGCA CAGAAACCAG CATCCGACAG 
AGCGAGACAA CCAACCATGG CTCTGCCGTC AGCACTGTCA CCAAAACACA GAGGCTTGTG 
CAGTCCAATG ATGGCAGCAA AACCTCTAGC ACAACTACAT TGGAGGCCAG TTACATGAAA 
CGATCAGAGA ATGGTGACAC CATTGTTCAG ACAAAATCAT CATTTAGTGC CACATCCAAG 
AAAGTTGGCA GCATCTTTGA CCGAGAGGAT GAGGGGCTTT CCAGGGCCAA CAGTATGACT 
GCAATGGAGC GGCGTCAGGC AGAAAGGAAA AAGGAGCTCA TGAAGGCTCA AAGCCTACCT 
AAAACGCCAG CCACGCAGGC ACGCAAGGCC ATGATTGAGA AGATGGAGAA GGAAAGTGGC 
GGCTCATCCA GCCCAGCCTT TGCCAAGGTT GCAACACCAC GTTCGGCTGC ATTTGGTGTG 
CCTAATGCAA ACAGCATAAA GCAAATGCTC TTGGATTGGT GCAAAGCCAA GACGCGTGGG 
TATGAGCATG TGAACATTCA GAACTTTTCC TCCAGCTGGA GCGATGGAAT GGCATTCTGT 
GCACTTGTCC ATAATTTCTT TCCTGAGGCA TTTGACTACA ACCAACTGAA CCCCCAGAAC 
AGAAGAAAGA ATTTTGACCT GGCCTTTTCA GCTGCAGAGA AACACGCAGA CTGTCCGCAG 
TTGCTGGACG CAGAGGATAT GGTCCGCATG CGAGAGCCGG ACTGGAAGTG TGTTTACACG 
TACATCCAGG AGTTCTACCG CTGCCTGGTG CAGAAGGGGA TGGTAAAAAC CAAAAAGTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812963.2
CDS	126..3128
Translation	MSGERFSCMDEGSLRKLLEVTLDLAERKEIRSAIRELRRKELERCEEALASKRFRSERSHEQENKENQPRADREEQQQRALNALTIRLQNINDLEELTSLLRNSKEYEERKLIRAAIRKVRNDEIEAASLGGMLIRQSSERDNGKLVCKSQDAVGNLVPSLAQEREDLEERQKIRAQIHELRSAQNGGSHIAGSKEIESNMLLRLKVPTKEQLPSSTSSSSSTSSFTSFSSEPSSSSHCQATELLSLSAEFEGQPHNNRNEVFMEPSAEDSGTETREQPNTHVASHKQNVKSEQKPLPVWTGRADSHIISRLGADNKESFPEGVTSASCSEPDDQTSMPPFRRAYSVRDRVKKFSEESSSVTTPALGFRFGSMRAERAPVRGAHVLQQHSLLSVGQENNQKAAQNARFVTKQEVTRTVSGPCTRRLDGIPTAGTQSTNKIQSFKSPFIPELNNSRSTSLKGSSSLSSTGPCTSSREVNRGLPQSQGSFILKQNSGRSQENQLKVPLPMTERGRIDENSDGANTEGPGSLQSSTHLCKSTKGTREETEDTDMKTLLTIEIKDGRNQTTSSRVVGQPGNQRAELMLGLTSSPFRINSGFSTSNDGASSGITTRSIRTETRTTTQSGITSSSNVIKVEAETPQETPQQLEVFLPSTTEKAEPENKGKLTAEDLNAIEEEEVLDKMLDKATDFEERKLIRAAMRDLRQRKREQREKEREQRLQELKNKERESQLARSTETSIRQSETTNHGSAVSTVTKTQRLVQSNDGSKTSSTTTLEASYMKRSENGDTIVQTKSSFSATSKKVGSIFDREDEGLSRANSMTAMERRQAERKKELMKAQSLPKTPATQARKAMIEKMEKESGGSSSPAFAKVATPRSAAFGVPNANSIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAEKHADCPQLLDAEDMVRMREPDWKCVYTYIQEFYRCLVQKGMVKTKKS

Target ORF information:

RefSeq Version	XM_012957509.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis smoothelin (smtn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957509.3

ORF Insert Sequence:

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GATGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GTCCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG TTGAGGCAGA AACTCCCCAG 
GAAACTCCCC AGCAACTGGA GGTATTTCTC CCCAGCACAA CAGAGAAGGC AGAACCAGAA 
AACAAGGGCA AGCTAACAGC AGAGGATCTG AATGCTATAG AGGAAGAGGA AGTTCTGGAT 
AAAATGCTGG ATAAGGCCAC AGACTTTGAG GAGAGGAAAC TAATCCGTGC GGCAATGAGG 
GATCTGCGTC AGAGAAAGAG AGAGCAACGA GAGAAAGAGC GAGAACAGCG CTTACAGGAG 
TTAAAGAACA AGGAGAGGGA ATCTCAGCTG GCACGAAGCA CAGAAACCAG CATCCGACAG 
AGCGAGACAA CCAACCATGG CTCTGCCGTC AGCACTGTCA CCAAAACACA GAGGCTTGTG 
CAGTCCAATG ATGGCAGCAA AACCTCTAGC ACAACTACAT TGGAGGCCAG TTACATGAAA 
CGATCAGAGA ATGGTGACAC CATTGTTCAG ACAAAATCAT CATTTAGTGC CACATCCAAG 
AAAGTTGGCA GCATCTTTGA CCGAGAGGAT GAGGGGCTTT CCAGGGCCAA CAGTATGACT 
GCAATGGAGC GGCGTCAGGC AGAAAGGAAA AAGGAGCTCA TGAAGGCTCA AAGCCTACCT 
AAAACGCCAG CCACGCAGGC ACGCAAGGCC ATGATTGAGA AGATGGAGAA GGAAAGTGGC 
GGCTCATCCA GCCCAGCCTT TGCCAAGGTT GCAACACCAC GTTCGGCTGC ATTTGGTGTG 
CCTAATGCAA ACAGCATAAA GCAAATGCTC TTGGATTGGT GCAAAGCCAA GACGCGTGGG 
TATGAGCATG TGAACATTCA GAACTTTTCC TCCAGCTGGA GCGATGGAAT GGCATTCTGT 
GCACTTGTCC ATAATTTCTT TCCTGAGGCA TTTGACTACA ACCAACTGAA CCCCCAGAAC 
AGAAGAAAGA ATTTTGACCT GGCCTTTTCA GCTGCAGAGA AACACGCAGA CTGTCCGCAG 
TTGCTGGACG CAGAGGATAT GGTCCGCATG CGAGAGCCGG ACTGGAAGTG TGTTTACACG 
TACATCCAGG AGTTCTACCG CTGCCTGGTG CAGAAGGGGA TGGTAAAAAC CAAAAAGTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smtn ( NM_001142131.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001135603.2 Xenopus tropicalis smoothelin (smtn), mRNA.

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CloneID	OXa21468
Clone ID Related Accession (Same CDS sequence)	NM_001142131.2
Accession Version	NM_001142131.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	smoothelin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX913357.1, BC168420.1, CT030397.1, CX485777.2, CX362831.2, DT393913.1 and BX770564.1. On Oct 25, 2011 this sequence version replaced NM_001142131.1. ##Evidence-Data-START## Transcript exon combination :: BC168420.1, CT030397.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028293 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GGTGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GACCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG TTGAGGCAGA AACTCCCCAG 
GAAACTCCCC AGCAACTGGA GGTATTTCTC CCCAGCACAA CAGAGAAGGC AGAACCAGAA 
AACAAGGGCA AGCTAACAGC AGAGGATCTG AATGCTATAG AGGAAGAGGA AGTTCTGGAT 
AAAATGCTGG ATAAGGCCAC AGACTTTGAG GAGAGGAAAC TAATCCGTGC GGCAATGAGG 
GATCTGCGTC AGAGAAAGAG AGAGCAACGA GAGAAAGAGC GAGAACAGCG CTTACAGGAG 
TTAAAGAACA AGGAGAGGGA ATCAAAGCTG GCACGAAGCA CAGAAACCAG CATCCGACAG 
AGCGAGACAA CCAACCATGG CTCTGCCGTC AGCACTGTCA CCAAAACACA GAGGCTTGTG 
CAGTCCAATG ATGGCAGCAA AACCTCTAGC ACAACTACAT TGGAGGCCAG TTACATGAAA 
CGATCAGAGA ATGGTGACAC CATTGTTCAG ACAAAATCAT CATTTAGTGC CACATCCAAG 
AAAGTTGGCA GCATCTTTGA CCGAGAGGAT GAGGGGCTTT CCAGGGCCAA CAGTATGACT 
GCAATGGAGC GGCGTCAGGC AGAAAGGAAA AAGGAGCTCA TGAAGGCTCA AAGCCTACCT 
AAAACGCCAG CCACGCAGGC ACGCAAGGCC ATGATTGAGA AGATGGAGAA GGAAAGTGGC 
GGCTCATCCA GCCCAGCCTT TGCCAAGGTT GCAACACCAC GTTCGGCTGC ATTTGGTGTG 
CCTAATGCAA ACAGCATAAA GCAAATGCTC TTGGATTGGT GCAAAGCCAA GACGCGTGGG 
TATGAGCATG TGAACATTCA GAACTTTTCC TCCAGCTGGA GCGATGGAAT GGCATTCTGT 
GCACTTGTCC ATAATTTCTT TCCTGAGGCA TTTGACTACA ACCAACTGAA CCCCCAGAAC 
AGAAGAAAGA ATTTTGACCT GGCCTTTTCA GCTGCAGAGA TGCTTGTGGA CTGTGTCCCT 
CTGGTGGAGG TGGAGGACAT GATGATCATG GGGAAGAGAC CAGACTCCAA GTGTGTCTTC 
ACTTACGTCC AGTCCTTGTA CAACCACCTG CGGCGGCATG AGATGCGTCT ACGCAAGTCA 
GAGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135603.2
CDS	115..3123
Translation	MSGERFSCMDEGSLRKLLEVTLDLAERKEIRSAIRELRRKELERCEEALASKRFRSERSHEQENKENQPRADREEQQQRALNALTIRLQNINDLEELTSLLRNSKEYEERKLIRAAIRKVRNDEIEAASLGGMLIRQSSERDNGKLVCKSQDAVGNLVPSLAQEREDLEERQKIRAQIHELRSAQNGGSHIAGSKEIESNMLLRLKVPTKEQLPSSTSSSSSTSSFTSFSSEPSSSSHCQATELLSLSAEFEGQPHNNRNEVFMEPSAEDSGTETREQPNTHVASHKQNVKSEQKPLPVWTGRADSHIISRLGADNKESFPEGVTSASCSEPDDQTSMPPFRRAYSVRDRVKKFSEETSSVTTPALGFRFGSMRAERAPVRGAHVLQQHSLLSVGQENNQKAAQNARFVTKQEVTRTVSGPCTRRLDGIPTAGTQSTNKIQSFKSPFIPELNNSRSTSLKGSSSLSSTGPCTSSREVNRGLPQSQGSFILKQNSGRSQENQLKVPLPMTERGRIDENSDGANTEGPGSLQSSTHLCKSTKGTREETEDTDMKTLLTIEIKDGRNQTTSSRVVGQPGNQRAELMLGLTSSPFRINSGFSTSNDGASSGITTRSIRTETRTTTQSGITSSSNVIKVEAETPQETPQQLEVFLPSTTEKAEPENKGKLTAEDLNAIEEEEVLDKMLDKATDFEERKLIRAAMRDLRQRKREQREKEREQRLQELKNKERESKLARSTETSIRQSETTNHGSAVSTVTKTQRLVQSNDGSKTSSTTTLEASYMKRSENGDTIVQTKSSFSATSKKVGSIFDREDEGLSRANSMTAMERRQAERKKELMKAQSLPKTPATQARKAMIEKMEKESGGSSSPAFAKVATPRSAAFGVPNANSIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAEMLVDCVPLVEVEDMMIMGKRPDSKCVFTYVQSLYNHLRRHEMRLRKSEF

Target ORF information:

RefSeq Version	NM_001142131.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis smoothelin (smtn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142131.2

ORF Insert Sequence:

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GGTGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GACCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG TTGAGGCAGA AACTCCCCAG 
GAAACTCCCC AGCAACTGGA GGTATTTCTC CCCAGCACAA CAGAGAAGGC AGAACCAGAA 
AACAAGGGCA AGCTAACAGC AGAGGATCTG AATGCTATAG AGGAAGAGGA AGTTCTGGAT 
AAAATGCTGG ATAAGGCCAC AGACTTTGAG GAGAGGAAAC TAATCCGTGC GGCAATGAGG 
GATCTGCGTC AGAGAAAGAG AGAGCAACGA GAGAAAGAGC GAGAACAGCG CTTACAGGAG 
TTAAAGAACA AGGAGAGGGA ATCAAAGCTG GCACGAAGCA CAGAAACCAG CATCCGACAG 
AGCGAGACAA CCAACCATGG CTCTGCCGTC AGCACTGTCA CCAAAACACA GAGGCTTGTG 
CAGTCCAATG ATGGCAGCAA AACCTCTAGC ACAACTACAT TGGAGGCCAG TTACATGAAA 
CGATCAGAGA ATGGTGACAC CATTGTTCAG ACAAAATCAT CATTTAGTGC CACATCCAAG 
AAAGTTGGCA GCATCTTTGA CCGAGAGGAT GAGGGGCTTT CCAGGGCCAA CAGTATGACT 
GCAATGGAGC GGCGTCAGGC AGAAAGGAAA AAGGAGCTCA TGAAGGCTCA AAGCCTACCT 
AAAACGCCAG CCACGCAGGC ACGCAAGGCC ATGATTGAGA AGATGGAGAA GGAAAGTGGC 
GGCTCATCCA GCCCAGCCTT TGCCAAGGTT GCAACACCAC GTTCGGCTGC ATTTGGTGTG 
CCTAATGCAA ACAGCATAAA GCAAATGCTC TTGGATTGGT GCAAAGCCAA GACGCGTGGG 
TATGAGCATG TGAACATTCA GAACTTTTCC TCCAGCTGGA GCGATGGAAT GGCATTCTGT 
GCACTTGTCC ATAATTTCTT TCCTGAGGCA TTTGACTACA ACCAACTGAA CCCCCAGAAC 
AGAAGAAAGA ATTTTGACCT GGCCTTTTCA GCTGCAGAGA TGCTTGTGGA CTGTGTCCCT 
CTGGTGGAGG TGGAGGACAT GATGATCATG GGGAAGAGAC CAGACTCCAA GTGTGTCTTC 
ACTTACGTCC AGTCCTTGTA CAACCACCTG CGGCGGCATG AGATGCGTCT ACGCAAGTCA 
GAGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smtn ( XM_012957509.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812963.1 Xenopus tropicalis smoothelin (smtn), transcript variant X3, mRNA.

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CloneID	OXa32649
Clone ID Related Accession (Same CDS sequence)	XM_012957509.2
Accession Version	XM_012957509.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	smoothelin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957509.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GGTGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GACCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG TTGAGGCAGA AACTCCCCAG 
GAAACTCCCC AGCAACTGGA GGTATTTCTC CCCAGCACAA CAGAGAAGGC AGAACCAGAA 
AACAAGGGCA AGCTAACAGC AGAGGATCTG AATGCTATAG AGGAAGAGGA AGTTCTGGAT 
AAAATGCTGG ATAAGGCCAC AGACTTTGAG GAGAGGAAAC TAATCCGTGC GGCAATGAGG 
GATCTGCGTC AGAGAAAGAG AGAGCAACGA GAGAAAGAGC GAGAACAGCG CTTACAGGAG 
TTAAAGAACA AGGAGAGGGA ATCAAAGCTG GCACGAAGCA CAGAAACCAG CATCCGACAG 
AGCGAGACAA CCAACCATGG CTCTGCCGTC AGCACTGTCA CCAAAACACA GAGGCTTGTG 
CAGTCCAATG ATGGCAGCAA AACCTCTAGC ACAACTACAT TGGAGGCCAG TTACATGAAA 
CGATCAGAGA ATGGTGACAC CATTGTTCAG ACAAAATCAT CATTTAGTGC CACATCCAAG 
AAAGTTGGCA GCATCTTTGA CCGAGAGGAT GAGGGGCTTT CCAGGGCCAA CAGTATGACT 
GCAATGGAGC GGCGTCAGGC AGAAAGGAAA AAGGAGCTCA TGAAGGCTCA AAGCCTACCT 
AAAACGCCAG CCACGCAGGC ACGCAAGGCC ATGATTGAGA AGATGGAGAA GGAAAGTGGC 
GGCTCATCCA GCCCAGCCTT TGCCAAGGTT GCAACACCAC GTTCGGCTGC ATTTGGTGTG 
CCTAATGCAA ACAGCATAAA GCAAATGCTC TTGGATTGGT GCAAAGCCAA GACGCGTGGG 
TATGAGCATG TGAACATTCA GAACTTTTCC TCCAGCTGGA GCGATGGAAT GGCATTCTGT 
GCACTTGTCC ATAATTTCTT TCCTGAGGCA TTTGACTACA ACCAACTGAA CCCCCAGAAC 
AGAAGAAAGA ATTTTGACCT GGCCTTTTCA GCTGCAGAGA AACACGCAGA CTGTCCGCAG 
TTGCTGGACG CAGAGGATAT GGTCCGCATG CGAGAGCCGG ACTGGAAGTG TGTTTACACG 
TACATCCAGG AGTTCTACCG CTGCCTGGTG CAGAAGGGGA TGGTAAAAAC CAAAAAGTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812963.1
CDS	134..3136
Translation	MSGERFSCMDEGSLRKLLEVTLDLAERKEIRSAIRELRRKELERCEEALASKRFRSERSHEQENKENQPRADREEQQQRALNALTIRLQNINDLEELTSLLRNSKEYEERKLIRAAIRKVRNDEIEAASLGGMLIRQSSERDNGKLVCKSQDAVGNLVPSLAQEREDLEERQKIRAQIHELRSAQNGGSHIAGSKEIESNMLLRLKVPTKEQLPSSTSSSSSTSSFTSFSSEPSSSSHCQATELLSLSAEFEGQPHNNRNEVFMEPSAEDSGTETREQPNTHVASHKQNVKSEQKPLPVWTGRADSHIISRLGADNKESFPEGVTSASCSEPDDQTSMPPFRRAYSVRDRVKKFSEETSSVTTPALGFRFGSMRAERAPVRGAHVLQQHSLLSVGQENNQKAAQNARFVTKQEVTRTVSGPCTRRLDGIPTAGTQSTNKIQSFKSPFIPELNNSRSTSLKGSSSLSSTGPCTSSREVNRGLPQSQGSFILKQNSGRSQENQLKVPLPMTERGRIDENSDGANTEGPGSLQSSTHLCKSTKGTREETEDTDMKTLLTIEIKDGRNQTTSSRVVGQPGNQRAELMLGLTSSPFRINSGFSTSNDGASSGITTRSIRTETRTTTQSGITSSSNVIKVEAETPQETPQQLEVFLPSTTEKAEPENKGKLTAEDLNAIEEEEVLDKMLDKATDFEERKLIRAAMRDLRQRKREQREKEREQRLQELKNKERESKLARSTETSIRQSETTNHGSAVSTVTKTQRLVQSNDGSKTSSTTTLEASYMKRSENGDTIVQTKSSFSATSKKVGSIFDREDEGLSRANSMTAMERRQAERKKELMKAQSLPKTPATQARKAMIEKMEKESGGSSSPAFAKVATPRSAAFGVPNANSIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAEKHADCPQLLDAEDMVRMREPDWKCVYTYIQEFYRCLVQKGMVKTKKS

Target ORF information:

RefSeq Version	XM_012957509.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis smoothelin (smtn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957509.2

ORF Insert Sequence:

ATGTCTGGGG AAAGGTTCTC CTGTATGGAT GAGGGCAGCC TACGCAAGCT TCTGGAGGTG 
ACGCTGGACT TGGCAGAGCG GAAAGAGATT CGATCAGCTA TCCGTGAGCT ACGTAGAAAG 
GAACTGGAGA GGTGTGAAGA GGCACTGGCA TCTAAACGGT TTCGCTCAGA GAGGAGCCAT 
GAACAGGAGA ACAAGGAGAA CCAGCCAAGG GCTGACAGAG AGGAACAGCA GCAGAGAGCA 
CTGAATGCCC TGACTATAAG GCTTCAGAAT ATCAATGATC TGGAGGAGTT GACATCTCTG 
CTGCGGAATT CCAAAGAATA TGAGGAGAGA AAGCTTATCA GAGCAGCTAT TCGAAAAGTG 
CGAAACGATG AAATTGAGGC TGCATCACTG GGAGGGATGC TGATTAGACA AAGTAGTGAA 
CGGGACAATG GAAAGCTAGT CTGCAAAAGC CAGGATGCTG TTGGGAACCT TGTGCCATCT 
TTGGCACAGG AGAGAGAAGA CCTAGAAGAA AGGCAGAAAA TTAGAGCTCA GATTCATGAA 
CTGAGAAGTG CACAGAATGG TGGCAGCCAC ATAGCAGGAT CCAAAGAAAT AGAATCTAAT 
ATGCTGCTGC GCCTCAAAGT TCCTACTAAA GAACAATTGC CGTCTTCAAC ATCTTCTTCT 
TCATCCACCT CTTCATTCAC GTCTTTCTCA TCAGAACCAT CCAGTTCATC TCATTGTCAA 
GCTACAGAAC TCTTGTCTTT AAGTGCAGAG TTCGAAGGAC AACCCCACAA TAACAGAAAT 
GAGGTGTTTA TGGAGCCTTC AGCAGAAGAC AGTGGTACTG AGACAAGGGA ACAACCAAAT 
ACGCATGTGG CCTCCCACAA ACAGAATGTC AAGTCAGAAC AAAAGCCTTT GCCAGTATGG 
ACTGGCAGAG CTGATTCTCA CATCATCTCT AGGCTGGGAG CTGATAACAA GGAATCTTTT 
CCCGAGGGAG TGACCTCAGC GTCCTGCTCT GAACCAGATG ACCAGACCTC CATGCCTCCA 
TTTAGAAGAG CTTACTCAGT CAGAGACAGA GTAAAGAAGT TTTCTGAAGA GACCTCTAGT 
GTAACAACCC CTGCCTTGGG TTTCCGTTTT GGAAGCATGC GTGCTGAGAG AGCCCCAGTC 
AGAGGAGCAC ATGTACTGCA GCAGCATTCC CTATTATCTG TTGGACAAGA AAATAATCAG 
AAAGCTGCAC AAAATGCTAG ATTTGTCACT AAGCAAGAAG TCACACGTAC AGTTTCAGGG 
CCCTGTACCC GCAGGTTAGA TGGAATTCCT ACAGCTGGGA CACAGAGCAC TAACAAAATA 
CAGTCATTTA AATCTCCTTT CATACCTGAG TTAAACAATT CCAGGAGCAC GTCCTTAAAA 
GGATCCTCAT CTCTCAGCTC TACAGGACCC TGCACATCCT CGAGGGAAGT GAATAGAGGA 
CTTCCACAAA GCCAAGGTTC CTTCATCCTC AAGCAGAATA GTGGGAGGAG CCAAGAAAAC 
CAATTGAAAG TTCCTCTACC CATGACAGAA AGGGGGAGGA TAGATGAAAA CTCAGATGGA 
GCCAATACAG AGGGACCAGG CAGCCTACAA TCCTCCACAC ATCTCTGCAA ATCCACCAAA 
GGCACTCGAG AAGAGACAGA GGACACTGAC ATGAAGACAC TGCTTACCAT TGAAATTAAG 
GATGGGAGAA ATCAGACTAC TAGTAGTCGG GTGGTGGGTC AGCCTGGAAA CCAACGAGCA 
GAGTTGATGT TGGGACTCAC AAGTTCACCT TTTAGAATTA ACAGTGGCTT TAGCACAAGC 
AATGATGGAG CCAGCAGCGG GATCACTACA AGGAGCATAA GGACTGAGAC CAGGACCACC 
ACACAAAGTG GGATCACTTC CAGCAGTAAT GTCATCAAGG TTGAGGCAGA AACTCCCCAG 
GAAACTCCCC AGCAACTGGA GGTATTTCTC CCCAGCACAA CAGAGAAGGC AGAACCAGAA 
AACAAGGGCA AGCTAACAGC AGAGGATCTG AATGCTATAG AGGAAGAGGA AGTTCTGGAT 
AAAATGCTGG ATAAGGCCAC AGACTTTGAG GAGAGGAAAC TAATCCGTGC GGCAATGAGG 
GATCTGCGTC AGAGAAAGAG AGAGCAACGA GAGAAAGAGC GAGAACAGCG CTTACAGGAG 
TTAAAGAACA AGGAGAGGGA ATCAAAGCTG GCACGAAGCA CAGAAACCAG CATCCGACAG 
AGCGAGACAA CCAACCATGG CTCTGCCGTC AGCACTGTCA CCAAAACACA GAGGCTTGTG 
CAGTCCAATG ATGGCAGCAA AACCTCTAGC ACAACTACAT TGGAGGCCAG TTACATGAAA 
CGATCAGAGA ATGGTGACAC CATTGTTCAG ACAAAATCAT CATTTAGTGC CACATCCAAG 
AAAGTTGGCA GCATCTTTGA CCGAGAGGAT GAGGGGCTTT CCAGGGCCAA CAGTATGACT 
GCAATGGAGC GGCGTCAGGC AGAAAGGAAA AAGGAGCTCA TGAAGGCTCA AAGCCTACCT 
AAAACGCCAG CCACGCAGGC ACGCAAGGCC ATGATTGAGA AGATGGAGAA GGAAAGTGGC 
GGCTCATCCA GCCCAGCCTT TGCCAAGGTT GCAACACCAC GTTCGGCTGC ATTTGGTGTG 
CCTAATGCAA ACAGCATAAA GCAAATGCTC TTGGATTGGT GCAAAGCCAA GACGCGTGGG 
TATGAGCATG TGAACATTCA GAACTTTTCC TCCAGCTGGA GCGATGGAAT GGCATTCTGT 
GCACTTGTCC ATAATTTCTT TCCTGAGGCA TTTGACTACA ACCAACTGAA CCCCCAGAAC 
AGAAGAAAGA ATTTTGACCT GGCCTTTTCA GCTGCAGAGA AACACGCAGA CTGTCCGCAG 
TTGCTGGACG CAGAGGATAT GGTCCGCATG CGAGAGCCGG ACTGGAAGTG TGTTTACACG 
TACATCCAGG AGTTCTACCG CTGCCTGGTG CAGAAGGGGA TGGTAAAAAC CAAAAAGTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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smtn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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