nhsl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nhsl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nhsl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa32641 XM_012962807.3
Latest version!
Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32642 XM_012962809.3
Latest version!
Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa57932 XM_031901570.1
Latest version!
Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32637 XM_012962802.3
Latest version!
Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa21753 NM_001142125.1
Latest version!
Xenopus tropicalis NHS like 1 (nhsl1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa32635 XM_012962800.2
Latest version!
Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa32636 XM_012962801.2
Latest version!
Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa32638 XM_012962803.2
Latest version!
Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa32639 XM_012962805.2
Latest version!
Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32639 XM_012962804.2
Latest version!
Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OXa32641 XM_012962807.2 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32642 XM_012962809.2 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32637 XM_012962802.2 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa32641
    Clone ID Related Accession (Same CDS sequence) XM_012962807.2 , XM_012962807.3
    Accession Version XM_012962807.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962807.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GAGAGATGCC CAAAGAGATG
    CTCAGAAGCG GATCTGCCAG GGGGGCGGGG GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818261.1
    CDS636..4931
    Translation

    Target ORF information:

    RefSeq Version XM_012962807.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962807.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GAGAGATGCC CAAAGAGATG
    CTCAGAAGCG GATCTGCCAG GGGGGCGGGG GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32642
    Clone ID Related Accession (Same CDS sequence) XM_012962809.3 , XM_012962809.2
    Accession Version XM_012962809.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GTGTGTGCAC ATGTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818263.1
    CDS636..4895
    Translation

    Target ORF information:

    RefSeq Version XM_012962809.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962809.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GTGTGTGCAC ATGTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57932
    Clone ID Related Accession (Same CDS sequence) XM_031901570.1
    Accession Version XM_031901570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGTCAGG AGGCTCAGGC AGTACAGACA GATGTAATTC CTATAAACAT AACAGGGGAG 
    AATTTCAACC GCCAGGCCAG CTTTCAGCGG TCACTTATTT ATACAGACAC TGCCGTAAGA
    TGTTCGAAGA AAGTCAAAAG GAGAAAGACT ATAACAGGCG TCCCAAACAA CTTACAGAAG
    GAGCTAGCAG GTACTGGACA CTCTGACCTA AGAAGGAATT CAGTGCTCAT ACCTGATGAT
    TTTAATACTT TGGAACATTG TCACCACTCA ATGCAATTTC TGGACACCAG AGATTTTGGC
    TGCCAAACCA ATAAAATAAA AATAGTGCCA CCATCAATTA GAAGAATCAG AGCACAGAAA
    GGGCAAGGAA TTGGAATTCT GATGTCCACT TCCTCTGGCA ACATGTCTAC TCTAAGTGAC
    TCTGCAGCTT GTTCTTCATA TCTAAATGGT AACCTGCACT TCCGTAGTCT GCCCAGGATT
    GGTGCAAGAG AATCTCTTCA ATCCCTTAAC CAAAAAAATT ATTATTACAG CAACATGGAG
    GACCCTTTTA CAACTTTGCC TTGGCAGTTA AACAAACTGC AAGTGGATGA TACTAATGTT
    CAATTAAGGA GTAACCCTAG GTCAACTATT AATCAAAGGC CTGAGTCTCA AGAAGTAAAA
    AATTCTACTA GTGAAATTGC CTCAAGTCCA GCATGTATGG TCTCGCCTCA TTCTTCTTAT
    TCAACAAACA TTATTCCAAA TGCCATACTT TCATATTCCT CAGAAGTAAT AAGTATTCAC
    ACTCCCCACA GAGGTGGACT TCTGGACAAG AAACCAAGAG TCCACAGTTC ATCTTCTAAC
    AGAAATGGTA TTAGCAGGGA CTATACTTCT GGCATTAAAG ATAATTCCTC ACCTGGTAAC
    TGGCGTGAAA ACAGTTCATC TAGGAACTCA CAGATATCAG ATACAAAGTC ACCTATCAGT
    AAAAAAAGAG ATGTTCCACA CAACGTTCCT TGCAAAATTA ACACTGGCTT TAACAACTAC
    TCATTTGACA GTGACAATCA AAGTGAATTA AGCTACTCAG ATGGTCGTCA AAGAAATGGT
    AGTATAACTA GCAGCATTAA TAGTGCTGAC AACTGGTTGT ATGAATCTAG GCACAATGAC
    GGTGCTCCAT CTAGGAAACT ATCTTCTGTA ATTTCTGGGT CTCCTGTTAG CAATATGAGC
    GCCTGCAGTT CTAAGGGGAA GGTAGATTCC AGCTCTTTGT ATTTTCTGAA CCATGATGAT
    TTTTGCACAT CAATGCATAT AGATTCTGGA ATTAGTTTGA CGGACCAAAG CATTCAGAAT
    CTACACTATA ATATAAATAT CTTTGAAACA AAAAAAGCTG CAAATATAGA AGTGAAAACA
    GGATATAGTG ACAAATCACT TCCACGTAGC ATATCTTTAA AGAAATCTAA AAATCCTCCC
    CCCCCACCTT CCAGGACAGA CTCACTTAGA AGGCCATCTA AGAAAGATAT TCAGACCAGT
    GGGCAGGTGC TCAGTGAAAC TCTTATTGCT ACGCTTCAAC AGTCCTTGCA ACTAAACTTG
    AAGAGTAAGA GCACTGGGTC CACTTTGCAG TCAAGTCACT GTATGGATTA TGCAGATCCC
    TGCATCATGT GCTCATGTAG CCAGAGTACA CTTAGCAGCA CTAGTAGTGG AAAATCTGTT
    ATTGCTCCAA ATATGTACAC TCTCTGTACT GTGACACCCT CTCAGAGTGA AACCAGTAGC
    ATGAAATCTG AATATGCGGA TCAGTGGAGT TCCTTTATTG ACTGTTCACA GACTCCAAAA
    GAGCAACCCA AATCATTTGC AAAACACAAT CTAAGTAATC CTGATGGTCT TAATAATTAC
    AGTGCATGTG AGCTGACCTA TGGAATGAAG ACCATAGCAC CACATGGGTA CACTGGATCA
    GTTAAAGTTA GTAATTCCTC TTCAGAAAAA ACATACAGAG TTACATCACC ATCTAGTGGA
    TATTCAAGTC AGTCAAACAC TCCCACCACA TTGACACCAG TGCCTCTTTT CTCAAAGAAT
    ATATATCCAG AAAATGGAAA ATACAAGTTA AAACCAAAAG TACCTGAAAG ACGATCTTCA
    TTGGTATCCT CGGTTTCAGT TTCATCTTCC TCTACTTCCC TTTCTTCAAA TACATTGGAC
    TCTCTGCAGC AGAACATGCA AATTTCAACT GTTATGTTGT CAGCCCATCA CACTCTTCCA
    ACCCCACAAC TCACTCCTGA CATGCCCCCA CCCCCAGCAA TGAATGCAAC CAACACAATT
    TTTGTCGAGT GTTCACCAAA ATGTCCACCT CCTTCCCTTG AATTTACAAG GCTAACAGCG
    GATAGCATGG ATATGTGGCT TCCCTTCTCT CAAAACACTG CCATGAGTAA ACCTAAGGAC
    AGTCTTCCAT ATCCTTCCTT TCCACCTTCT CTTTCCATGA TGCACAGCAT GGTCCCATCA
    CCTGTACCTC CACTTAACAT TGAAATGCTG AAAATTGAAA CAGAATCATT TCCTCTCAGA
    AATTCTGAAA GCAGTTATTT TGTACATAAT GATCACAGTA AACATGAACT GTTGATACCA
    ACTGTTCCTA TAGTTACTTC TCATGCACTA GAAATGGTTC ATTTGAGATC TGTGAAAAAA
    GCATCACAAC TGGAAACTAA AACATTAAGA GAACACATGT CAGAATCCCA CCTACAGGAT
    GAGACATCCG CCTTATTGGT ACAGCCATGT TTAGTACCAG CTGCTTCTCC AGAACTAAAT
    TCTAGTGAAA GCCAAATAGA AAATTTCAGC ATTCCTGCCC AGCAAGTCTC TGGTTTTAAA
    AGCAATGCAG TGAAACTAAC TATTGGTGAA ATAGAAGCAG ACAAGGCATA TAAAGAGCAC
    ATAGAAAAAA ATACAAATTC AGCATACAAT GGAAACTCAT CAGGGCCCAA ATTATCTCCT
    CATGAGAAAC CAAGTATGTC ACAAAGCACA CCAAATTCAT CTCTTAGCAA GGAGCCGCCT
    CTTGTTTCAA AGAAGCCTAA ACTTGTACTT ATTGCCCCAA CGCCACAACT TGATTTGGCA
    GCAGAAAAAA TAGCTCAAGT CACTGAAAAT GTGAGAAGCA TGCCAAATGC CTTTGAAAAA
    GATTCAGTTA GCAAATGTTG TGTGGGGGAG ATAAATGACA GAACTAAAGA TGAAGACATA
    GCAGGTCCAA CCAATGGTTT GGCTCACCAA GAACAAGAAG CCTGGTTATC CCCATGCAAA
    GCTGCTAAAA ATATGATTTT CATACTACCA TCAGTTCCAG AAATTCAGAA TCATCAGCAA
    AAGGAAGAGC AGCAATCCAT GTCAGATGAA TTCAACATTA GTGGTAACAT TTCTGAGACT
    GAGAATACAA GCTGCAACCA GAATGCATCT CAAGATGATG AGAGTGCTGA AGTGTTTAAT
    ACAGAGACGG TGAATTCAGT TCTCTGGACA AGCAGCGACA TTATGGGAAA TGTAGAAGAG
    ACCACTCCAA CAAGGCCTGC TACAACTGAG GATCTTTTTG CTCGTATCCA TAGATCCAAA
    AAAAAAATTC TTGGACGCAA AGATTCTGAC GATGATCGTT GCAGAGATCA CTCGCCTTCT
    TCTCCAGTTA CCCCAACTGG TAATATGCAT CTAAGTTCCT TCAAGCAGAC AGGTTCAATC
    CAAAGAAGTG TCAGAAAAAC TACAACAAGT AGTGACAGTT TCAAAGCACT GCTGTTAAAA
    AAGGGAAGCA GGTCTGAAAG TGGCTTCCGC ATGTCTGCTG CAGAAATGCT GAAACATACT
    GATCCAAGGT TTCAGAGATC AAGATCAGAA TCATCTCTGG AGTTTCCAGA AAGCCCCACA
    TTGTCATCAC CAAACAAGAA CAAGAAGGAG CAACAGGAAT GGGCAAAAAG TGAAGGGCTG
    ATGCCTAGAA GTATGTCTGG CTCTGTTGTC CGGTGCAGCC GCGCCAAGAC TCCCCCTTCT
    GCATCTAGTA GCAAATACAA TGTCCGTAGT CGTCTCCAGA GTAGCCCAAT GACTGTTATA
    TGTGAAGGAG AAGGAGAGGG TTCAGAAACA GTGGATAGCA GCTGTTCAAA GGGTCCTCTT
    TTGTTGAGCA CAAAGAGTCT CGATATCTGT TACAGTGCTG ATAAAGAAGT GACCAACCAC
    ATACTGCACA TAAACTTTAA CACACTATTA GAAAAGAGCA CAGATGTTGC TAGTGGCTGC
    ACAGGGGAAA TAATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757430.1
    CDS309..4526
    Translation

    Target ORF information:

    RefSeq Version XM_031901570.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGTCAGG AGGCTCAGGC AGTACAGACA GATGTAATTC CTATAAACAT AACAGGGGAG 
    AATTTCAACC GCCAGGCCAG CTTTCAGCGG TCACTTATTT ATACAGACAC TGCCGTAAGA
    TGTTCGAAGA AAGTCAAAAG GAGAAAGACT ATAACAGGCG TCCCAAACAA CTTACAGAAG
    GAGCTAGCAG GTACTGGACA CTCTGACCTA AGAAGGAATT CAGTGCTCAT ACCTGATGAT
    TTTAATACTT TGGAACATTG TCACCACTCA ATGCAATTTC TGGACACCAG AGATTTTGGC
    TGCCAAACCA ATAAAATAAA AATAGTGCCA CCATCAATTA GAAGAATCAG AGCACAGAAA
    GGGCAAGGAA TTGGAATTCT GATGTCCACT TCCTCTGGCA ACATGTCTAC TCTAAGTGAC
    TCTGCAGCTT GTTCTTCATA TCTAAATGGT AACCTGCACT TCCGTAGTCT GCCCAGGATT
    GGTGCAAGAG AATCTCTTCA ATCCCTTAAC CAAAAAAATT ATTATTACAG CAACATGGAG
    GACCCTTTTA CAACTTTGCC TTGGCAGTTA AACAAACTGC AAGTGGATGA TACTAATGTT
    CAATTAAGGA GTAACCCTAG GTCAACTATT AATCAAAGGC CTGAGTCTCA AGAAGTAAAA
    AATTCTACTA GTGAAATTGC CTCAAGTCCA GCATGTATGG TCTCGCCTCA TTCTTCTTAT
    TCAACAAACA TTATTCCAAA TGCCATACTT TCATATTCCT CAGAAGTAAT AAGTATTCAC
    ACTCCCCACA GAGGTGGACT TCTGGACAAG AAACCAAGAG TCCACAGTTC ATCTTCTAAC
    AGAAATGGTA TTAGCAGGGA CTATACTTCT GGCATTAAAG ATAATTCCTC ACCTGGTAAC
    TGGCGTGAAA ACAGTTCATC TAGGAACTCA CAGATATCAG ATACAAAGTC ACCTATCAGT
    AAAAAAAGAG ATGTTCCACA CAACGTTCCT TGCAAAATTA ACACTGGCTT TAACAACTAC
    TCATTTGACA GTGACAATCA AAGTGAATTA AGCTACTCAG ATGGTCGTCA AAGAAATGGT
    AGTATAACTA GCAGCATTAA TAGTGCTGAC AACTGGTTGT ATGAATCTAG GCACAATGAC
    GGTGCTCCAT CTAGGAAACT ATCTTCTGTA ATTTCTGGGT CTCCTGTTAG CAATATGAGC
    GCCTGCAGTT CTAAGGGGAA GGTAGATTCC AGCTCTTTGT ATTTTCTGAA CCATGATGAT
    TTTTGCACAT CAATGCATAT AGATTCTGGA ATTAGTTTGA CGGACCAAAG CATTCAGAAT
    CTACACTATA ATATAAATAT CTTTGAAACA AAAAAAGCTG CAAATATAGA AGTGAAAACA
    GGATATAGTG ACAAATCACT TCCACGTAGC ATATCTTTAA AGAAATCTAA AAATCCTCCC
    CCCCCACCTT CCAGGACAGA CTCACTTAGA AGGCCATCTA AGAAAGATAT TCAGACCAGT
    GGGCAGGTGC TCAGTGAAAC TCTTATTGCT ACGCTTCAAC AGTCCTTGCA ACTAAACTTG
    AAGAGTAAGA GCACTGGGTC CACTTTGCAG TCAAGTCACT GTATGGATTA TGCAGATCCC
    TGCATCATGT GCTCATGTAG CCAGAGTACA CTTAGCAGCA CTAGTAGTGG AAAATCTGTT
    ATTGCTCCAA ATATGTACAC TCTCTGTACT GTGACACCCT CTCAGAGTGA AACCAGTAGC
    ATGAAATCTG AATATGCGGA TCAGTGGAGT TCCTTTATTG ACTGTTCACA GACTCCAAAA
    GAGCAACCCA AATCATTTGC AAAACACAAT CTAAGTAATC CTGATGGTCT TAATAATTAC
    AGTGCATGTG AGCTGACCTA TGGAATGAAG ACCATAGCAC CACATGGGTA CACTGGATCA
    GTTAAAGTTA GTAATTCCTC TTCAGAAAAA ACATACAGAG TTACATCACC ATCTAGTGGA
    TATTCAAGTC AGTCAAACAC TCCCACCACA TTGACACCAG TGCCTCTTTT CTCAAAGAAT
    ATATATCCAG AAAATGGAAA ATACAAGTTA AAACCAAAAG TACCTGAAAG ACGATCTTCA
    TTGGTATCCT CGGTTTCAGT TTCATCTTCC TCTACTTCCC TTTCTTCAAA TACATTGGAC
    TCTCTGCAGC AGAACATGCA AATTTCAACT GTTATGTTGT CAGCCCATCA CACTCTTCCA
    ACCCCACAAC TCACTCCTGA CATGCCCCCA CCCCCAGCAA TGAATGCAAC CAACACAATT
    TTTGTCGAGT GTTCACCAAA ATGTCCACCT CCTTCCCTTG AATTTACAAG GCTAACAGCG
    GATAGCATGG ATATGTGGCT TCCCTTCTCT CAAAACACTG CCATGAGTAA ACCTAAGGAC
    AGTCTTCCAT ATCCTTCCTT TCCACCTTCT CTTTCCATGA TGCACAGCAT GGTCCCATCA
    CCTGTACCTC CACTTAACAT TGAAATGCTG AAAATTGAAA CAGAATCATT TCCTCTCAGA
    AATTCTGAAA GCAGTTATTT TGTACATAAT GATCACAGTA AACATGAACT GTTGATACCA
    ACTGTTCCTA TAGTTACTTC TCATGCACTA GAAATGGTTC ATTTGAGATC TGTGAAAAAA
    GCATCACAAC TGGAAACTAA AACATTAAGA GAACACATGT CAGAATCCCA CCTACAGGAT
    GAGACATCCG CCTTATTGGT ACAGCCATGT TTAGTACCAG CTGCTTCTCC AGAACTAAAT
    TCTAGTGAAA GCCAAATAGA AAATTTCAGC ATTCCTGCCC AGCAAGTCTC TGGTTTTAAA
    AGCAATGCAG TGAAACTAAC TATTGGTGAA ATAGAAGCAG ACAAGGCATA TAAAGAGCAC
    ATAGAAAAAA ATACAAATTC AGCATACAAT GGAAACTCAT CAGGGCCCAA ATTATCTCCT
    CATGAGAAAC CAAGTATGTC ACAAAGCACA CCAAATTCAT CTCTTAGCAA GGAGCCGCCT
    CTTGTTTCAA AGAAGCCTAA ACTTGTACTT ATTGCCCCAA CGCCACAACT TGATTTGGCA
    GCAGAAAAAA TAGCTCAAGT CACTGAAAAT GTGAGAAGCA TGCCAAATGC CTTTGAAAAA
    GATTCAGTTA GCAAATGTTG TGTGGGGGAG ATAAATGACA GAACTAAAGA TGAAGACATA
    GCAGGTCCAA CCAATGGTTT GGCTCACCAA GAACAAGAAG CCTGGTTATC CCCATGCAAA
    GCTGCTAAAA ATATGATTTT CATACTACCA TCAGTTCCAG AAATTCAGAA TCATCAGCAA
    AAGGAAGAGC AGCAATCCAT GTCAGATGAA TTCAACATTA GTGGTAACAT TTCTGAGACT
    GAGAATACAA GCTGCAACCA GAATGCATCT CAAGATGATG AGAGTGCTGA AGTGTTTAAT
    ACAGAGACGG TGAATTCAGT TCTCTGGACA AGCAGCGACA TTATGGGAAA TGTAGAAGAG
    ACCACTCCAA CAAGGCCTGC TACAACTGAG GATCTTTTTG CTCGTATCCA TAGATCCAAA
    AAAAAAATTC TTGGACGCAA AGATTCTGAC GATGATCGTT GCAGAGATCA CTCGCCTTCT
    TCTCCAGTTA CCCCAACTGG TAATATGCAT CTAAGTTCCT TCAAGCAGAC AGGTTCAATC
    CAAAGAAGTG TCAGAAAAAC TACAACAAGT AGTGACAGTT TCAAAGCACT GCTGTTAAAA
    AAGGGAAGCA GGTCTGAAAG TGGCTTCCGC ATGTCTGCTG CAGAAATGCT GAAACATACT
    GATCCAAGGT TTCAGAGATC AAGATCAGAA TCATCTCTGG AGTTTCCAGA AAGCCCCACA
    TTGTCATCAC CAAACAAGAA CAAGAAGGAG CAACAGGAAT GGGCAAAAAG TGAAGGGCTG
    ATGCCTAGAA GTATGTCTGG CTCTGTTGTC CGGTGCAGCC GCGCCAAGAC TCCCCCTTCT
    GCATCTAGTA GCAAATACAA TGTCCGTAGT CGTCTCCAGA GTAGCCCAAT GACTGTTATA
    TGTGAAGGAG AAGGAGAGGG TTCAGAAACA GTGGATAGCA GCTGTTCAAA GGGTCCTCTT
    TTGTTGAGCA CAAAGAGTCT CGATATCTGT TACAGTGCTG ATAAAGAAGT GACCAACCAC
    ATACTGCACA TAAACTTTAA CACACTATTA GAAAAGAGCA CAGATGTTGC TAGTGGCTGC
    ACAGGGGAAA TAATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32637
    Clone ID Related Accession (Same CDS sequence) XM_012962802.2 , XM_012962802.3
    Accession Version XM_012962802.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962802.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTTATAT TCGTTAATGC AATGCTGAAA TCATTTATTA GTATGTTCAA GAAAAAAACG 
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT
    GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC
    CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC
    AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG
    TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT
    CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA
    AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT
    ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC
    AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA
    GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA
    AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA
    AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA
    ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818256.1
    CDS294..4979
    Translation

    Target ORF information:

    RefSeq Version XM_012962802.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962802.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTTATAT TCGTTAATGC AATGCTGAAA TCATTTATTA GTATGTTCAA GAAAAAAACG 
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT
    GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC
    CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC
    AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG
    TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT
    CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA
    AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT
    ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC
    AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA
    GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA
    AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA
    AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA
    ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa21753
    Clone ID Related Accession (Same CDS sequence) NM_001142125.1
    Accession Version NM_001142125.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168125.1. ##Evidence-Data-START## Transcript exon combination :: BC168125.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTGCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCGAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGATAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT
    GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC
    CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC
    AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG
    TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT
    CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA
    AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT
    ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC
    AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA
    GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA
    AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA
    AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA
    ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001135597.1
    CDS252..5177
    Translation

    Target ORF information:

    RefSeq Version NM_001142125.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142125.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTGCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCGAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGATAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT
    GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC
    CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC
    AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG
    TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT
    CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA
    AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT
    ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC
    AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA
    GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA
    AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA
    AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA
    ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32635
    Clone ID Related Accession (Same CDS sequence) XM_012962800.2
    Accession Version XM_012962800.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4794bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGCAGGTAC TGGACACTCT
    GACCTAAGAA GGAATTCAGT GCTCATACCT GATGATTTTA ATACTTTGGA ACATTGTCAC
    CACTCAATGC AATTTCTGGA CACCAGAGAT TTTGGCTGCC AAACCAATAA AATAAAAATA
    GTGCCACCAT CAATTAGAAG AATCAGAGCA CAGAAAGGGC AAGGAATTGG AATTCTGATG
    TCCACTTCCT CTGGCAACAT GTCTACTCTA AGTGACTCTG CAGCTTGTTC TTCATATCTA
    AATGGTAACC TGCACTTCCG TAGTCTGCCC AGGATTGGTG CAAGAGAATC TCTTCAATCC
    CTTAACCAAA AAAATTATTA TTACAGCAAC ATGGAGGACC CTTTTACAAC TTTGCCTTGG
    CAGTTAAACA AACTGCAAGT GGATGATACT AATGTTCAAT TAAGGAGTAA CCCTAGGTCA
    ACTATTAATC AAAGGCCTGA GTCTCAAGAA GTAAAAAATT CTACTAGTGA AATTGCCTCA
    AGTCCAGCAT GTATGGTCTC GCCTCATTCT TCTTATTCAA CAAACATTAT TCCAAATGCC
    ATACTTTCAT ATTCCTCAGA AGTAATAAGT ATTCACACTC CCCACAGAGG TGGACTTCTG
    GACAAGAAAC CAAGAGTCCA CAGTTCATCT TCTAACAGAA ATGGTATTAG CAGGGACTAT
    ACTTCTGGCA TTAAAGATAA TTCCTCACCT GGTAACTGGC GTGAAAACAG TTCATCTAGG
    AACTCACAGA TATCAGATAC AAAGTCACCT ATCAGTAAAA AAAGAGATGT TCCACACAAC
    GTTCCTTGCA AAATTAACAC TGGCTTTAAC AACTACTCAT TTGACAGTGA CAATCAAAGT
    GAATTAAGCT ACTCAGATGG TCGTCAAAGA AATGGTAGTA TAACTAGCAG CATTAATAGT
    GCTGACAACT GGTTGTATGA ATCTAGGCAC AATGACGGTG CTCCATCTAG GAAACTATCT
    TCTGTAATTT CTGGGTCTCC TGTTAGCAAT ATGAGCGCCT GCAGTTCTAA GGGGAAGGTA
    GATTCCAGCT CTTTGTATTT TCTGAACCAT GATGATTTTT GCACATCAAT GCATATAGAT
    TCTGGAATTA GTTTGACGGA CCAAAGCATT CAGAATCTAC ACTATAATAT AAATATCTTT
    GAAACAAAAA AAGCTGCAAA TATAGAAGTG AAAACAGGAT ATAGTGACAA ATCACTTCCA
    CGTAGCATAT CTTTAAAGAA ATCTAAAAAT CCTCCCCCCC CACCTTCCAG GACAGACTCA
    CTTAGAAGGC CATCTAAGAA AGATATTCAG ACCAGTGGGC AGGTGCTCAG TGAAACTCTT
    ATTGCTACGC TTCAACAGTC CTTGCAACTA AACTTGAAGA GTAAGAGCAC TGGGTCCACT
    TTGCAGTCAA GTCACTGTAT GGATTATGCA GATCCCTGCA TCATGTGCTC ATGTAGCCAG
    AGTACACTTA GCAGCACTAG TAGTGGAAAA TCTGTTATTG CTCCAAATAT GTACACTCTC
    TGTACTGTGA CACCCTCTCA GAGTGAAACC AGTAGCATGA AATCTGAATA TGCGGATCAG
    TGGAGTTCCT TTATTGACTG TTCACAGACT CCAAAAGAGC AACCCAAATC ATTTGCAAAA
    CACAATCTAA GTAATCCTGA TGGTCTTAAT AATTACAGTG CATGTGAGCT GACCTATGGA
    ATGAAGACCA TAGCACCACA TGGGTACACT GGATCAGTTA AAGTTAGTAA TTCCTCTTCA
    GAAAAAACAT ACAGAGTTAC ATCACCATCT AGTGGATATT CAAGTCAGTC AAACACTCCC
    ACCACATTGA CACCAGTGCC TCTTTTCTCA AAGAATATAT ATCCAGAAAA TGGAAAATAC
    AAGTTAAAAC CAAAAGTACC TGAAAGACGA TCTTCATTGG TATCCTCGGT TTCAGTTTCA
    TCTTCCTCTA CTTCCCTTTC TTCAAATACA TTGGACTCTC TGCAGCAGAA CATGCAAATT
    TCAACTGTTA TGTTGTCAGC CCATCACACT CTTCCAACCC CACAACTCAC TCCTGACATG
    CCCCCACCCC CAGCAATGAA TGCAACCAAC ACAATTTTTG TCGAGTGTTC ACCAAAATGT
    CCACCTCCTT CCCTTGAATT TACAAGGCTA ACAGCGGATA GCATGGATAT GTGGCTTCCC
    TTCTCTCAAA ACACTGCCAT GAGTAAACCT AAGGACAGTC TTCCATATCC TTCCTTTCCA
    CCTTCTCTTT CCATGATGCA CAGCATGGTC CCATCACCTG TACCTCCACT TAACATTGAA
    ATGCTGAAAA TTGAAACAGA ATCATTTCCT CTCAGAAATT CTGAAAGCAG TTATTTTGTA
    CATAATGATC ACAGTAAACA TGAACTGTTG ATACCAACTG TTCCTATAGT TACTTCTCAT
    GCACTAGAAA TGGTTCATTT GAGATCTGTG AAAAAAGCAT CACAACTGGA AACTAAAACA
    TTAAGAGAAC ACATGTCAGA ATCCCACCTA CAGGATGAGA CATCCGCCTT ATTGGTACAG
    CCATGTTTAG TACCAGCTGC TTCTCCAGAA CTAAATTCTA GTGAAAGCCA AATAGAAAAT
    TTCAGCATTC CTGCCCAGCA AGTCTCTGGT TTTAAAAGCA ATGCAGTGAA ACTAACTATT
    GGTGAAATAG AAGCAGACAA GGCATATAAA GAGCACATAG AAAAAAATAC AAATTCAGCA
    TACAATGGAA ACTCATCAGG GCCCAAATTA TCTCCTCATG AGAAACCAAG TATGTCACAA
    AGCACACCAA ATTCATCTCT TAGCAAGGAG CCGCCTCTTG TTTCAAAGAA GCCTAAACTT
    GTACTTATTG CCCCAACGCC ACAACTTGAT TTGGCAGCAG AAAAAATAGC TCAAGTCACT
    GAAAATGTGA GAAGCATGCC AAATGCCTTT GAAAAAGATT CAGTTAGCAA ATGTTGTGTG
    GGGGAGATAA ATGACAGAAC TAAAGATGAA GACATAGCAG GTCCAACCAA TGGTTTGGCT
    CACCAAGAAC AAGAAGCCTG GTTATCCCCA TGCAAAGCTG CTAAAAATAT GATTTTCATA
    CTACCATCAG TTCCAGAAAT TCAGAATCAT CAGCAAAAGG AAGAGCAGCA ATCCATGTCA
    GATGAATTCA ACATTAGTGG TAACATTTCT GAGACTGAGA ATACAAGCTG CAACCAGAAT
    GCATCTCAAG ATGATGAGAG TGCTGAAGTG TTTAATACAG AGACGGTGAA TTCAGTTCTC
    TGGACAAGCA GCGACATTAT GGGAAATGTA GAAGAGACCA CTCCAACAAG GCCTGCTACA
    ACTGAGGATC TTTTTGCTCG TATCCATAGA TCCAAAAAAA AAATTCTTGG ACGCAAAGAT
    TCTGACGATG ATCGTTGCAG AGATCACTCG CCTTCTTCTC CAGTTACCCC AACTGGTAAT
    ATGCATCTAA GTTCCTTCAA GCAGACAGGT TCAATCCAAA GAAGTGTCAG AAAAACTACA
    ACAAGTAGTG ACAGTTTCAA AGCACTGCTG TTAAAAAAGG GAAGCAGGTC TGAAAGTGGC
    TTCCGCATGT CTGCTGCAGA AATGCTGAAA CATACTGATC CAAGGTTTCA GAGATCAAGA
    TCAGAATCAT CTCTGGAGTT TCCAGAAAGC CCCACATTGT CATCACCAAA CAAGAACAAG
    AAGGAGCAAC AGGAATGGGC AAAAAGTGAA GGGCTGATGC CTAGAAGTAT GTCTGGCTCT
    GTTGTCCGGT GCAGCCGCGC CAAGACTCCC CCTTCTGCAT CTAGTAGCAA ATACAATGTC
    CGTAGTCGTC TCCAGAGTAG CCCAATGACT GTTATATGTG AAGGAGAAGG AGAGGGTTCA
    GAAACAGTGG ATAGCAGCTG TTCAAAGGGT CCTCTTTTGT TGAGCACAAA GAGTCTCGAT
    ATCTGTTACA GTGCTGATAA AGAAGTGACC AACCACATAC TGCACATAAA CTTTAACACA
    CTATTAGAAA AGAGCACAGA TGTTGCTAGT GGCTGCACAG GGGAAATAAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818254.1
    CDS636..5429
    Translation

    Target ORF information:

    RefSeq Version XM_012962800.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962800.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGCAGGTAC TGGACACTCT
    GACCTAAGAA GGAATTCAGT GCTCATACCT GATGATTTTA ATACTTTGGA ACATTGTCAC
    CACTCAATGC AATTTCTGGA CACCAGAGAT TTTGGCTGCC AAACCAATAA AATAAAAATA
    GTGCCACCAT CAATTAGAAG AATCAGAGCA CAGAAAGGGC AAGGAATTGG AATTCTGATG
    TCCACTTCCT CTGGCAACAT GTCTACTCTA AGTGACTCTG CAGCTTGTTC TTCATATCTA
    AATGGTAACC TGCACTTCCG TAGTCTGCCC AGGATTGGTG CAAGAGAATC TCTTCAATCC
    CTTAACCAAA AAAATTATTA TTACAGCAAC ATGGAGGACC CTTTTACAAC TTTGCCTTGG
    CAGTTAAACA AACTGCAAGT GGATGATACT AATGTTCAAT TAAGGAGTAA CCCTAGGTCA
    ACTATTAATC AAAGGCCTGA GTCTCAAGAA GTAAAAAATT CTACTAGTGA AATTGCCTCA
    AGTCCAGCAT GTATGGTCTC GCCTCATTCT TCTTATTCAA CAAACATTAT TCCAAATGCC
    ATACTTTCAT ATTCCTCAGA AGTAATAAGT ATTCACACTC CCCACAGAGG TGGACTTCTG
    GACAAGAAAC CAAGAGTCCA CAGTTCATCT TCTAACAGAA ATGGTATTAG CAGGGACTAT
    ACTTCTGGCA TTAAAGATAA TTCCTCACCT GGTAACTGGC GTGAAAACAG TTCATCTAGG
    AACTCACAGA TATCAGATAC AAAGTCACCT ATCAGTAAAA AAAGAGATGT TCCACACAAC
    GTTCCTTGCA AAATTAACAC TGGCTTTAAC AACTACTCAT TTGACAGTGA CAATCAAAGT
    GAATTAAGCT ACTCAGATGG TCGTCAAAGA AATGGTAGTA TAACTAGCAG CATTAATAGT
    GCTGACAACT GGTTGTATGA ATCTAGGCAC AATGACGGTG CTCCATCTAG GAAACTATCT
    TCTGTAATTT CTGGGTCTCC TGTTAGCAAT ATGAGCGCCT GCAGTTCTAA GGGGAAGGTA
    GATTCCAGCT CTTTGTATTT TCTGAACCAT GATGATTTTT GCACATCAAT GCATATAGAT
    TCTGGAATTA GTTTGACGGA CCAAAGCATT CAGAATCTAC ACTATAATAT AAATATCTTT
    GAAACAAAAA AAGCTGCAAA TATAGAAGTG AAAACAGGAT ATAGTGACAA ATCACTTCCA
    CGTAGCATAT CTTTAAAGAA ATCTAAAAAT CCTCCCCCCC CACCTTCCAG GACAGACTCA
    CTTAGAAGGC CATCTAAGAA AGATATTCAG ACCAGTGGGC AGGTGCTCAG TGAAACTCTT
    ATTGCTACGC TTCAACAGTC CTTGCAACTA AACTTGAAGA GTAAGAGCAC TGGGTCCACT
    TTGCAGTCAA GTCACTGTAT GGATTATGCA GATCCCTGCA TCATGTGCTC ATGTAGCCAG
    AGTACACTTA GCAGCACTAG TAGTGGAAAA TCTGTTATTG CTCCAAATAT GTACACTCTC
    TGTACTGTGA CACCCTCTCA GAGTGAAACC AGTAGCATGA AATCTGAATA TGCGGATCAG
    TGGAGTTCCT TTATTGACTG TTCACAGACT CCAAAAGAGC AACCCAAATC ATTTGCAAAA
    CACAATCTAA GTAATCCTGA TGGTCTTAAT AATTACAGTG CATGTGAGCT GACCTATGGA
    ATGAAGACCA TAGCACCACA TGGGTACACT GGATCAGTTA AAGTTAGTAA TTCCTCTTCA
    GAAAAAACAT ACAGAGTTAC ATCACCATCT AGTGGATATT CAAGTCAGTC AAACACTCCC
    ACCACATTGA CACCAGTGCC TCTTTTCTCA AAGAATATAT ATCCAGAAAA TGGAAAATAC
    AAGTTAAAAC CAAAAGTACC TGAAAGACGA TCTTCATTGG TATCCTCGGT TTCAGTTTCA
    TCTTCCTCTA CTTCCCTTTC TTCAAATACA TTGGACTCTC TGCAGCAGAA CATGCAAATT
    TCAACTGTTA TGTTGTCAGC CCATCACACT CTTCCAACCC CACAACTCAC TCCTGACATG
    CCCCCACCCC CAGCAATGAA TGCAACCAAC ACAATTTTTG TCGAGTGTTC ACCAAAATGT
    CCACCTCCTT CCCTTGAATT TACAAGGCTA ACAGCGGATA GCATGGATAT GTGGCTTCCC
    TTCTCTCAAA ACACTGCCAT GAGTAAACCT AAGGACAGTC TTCCATATCC TTCCTTTCCA
    CCTTCTCTTT CCATGATGCA CAGCATGGTC CCATCACCTG TACCTCCACT TAACATTGAA
    ATGCTGAAAA TTGAAACAGA ATCATTTCCT CTCAGAAATT CTGAAAGCAG TTATTTTGTA
    CATAATGATC ACAGTAAACA TGAACTGTTG ATACCAACTG TTCCTATAGT TACTTCTCAT
    GCACTAGAAA TGGTTCATTT GAGATCTGTG AAAAAAGCAT CACAACTGGA AACTAAAACA
    TTAAGAGAAC ACATGTCAGA ATCCCACCTA CAGGATGAGA CATCCGCCTT ATTGGTACAG
    CCATGTTTAG TACCAGCTGC TTCTCCAGAA CTAAATTCTA GTGAAAGCCA AATAGAAAAT
    TTCAGCATTC CTGCCCAGCA AGTCTCTGGT TTTAAAAGCA ATGCAGTGAA ACTAACTATT
    GGTGAAATAG AAGCAGACAA GGCATATAAA GAGCACATAG AAAAAAATAC AAATTCAGCA
    TACAATGGAA ACTCATCAGG GCCCAAATTA TCTCCTCATG AGAAACCAAG TATGTCACAA
    AGCACACCAA ATTCATCTCT TAGCAAGGAG CCGCCTCTTG TTTCAAAGAA GCCTAAACTT
    GTACTTATTG CCCCAACGCC ACAACTTGAT TTGGCAGCAG AAAAAATAGC TCAAGTCACT
    GAAAATGTGA GAAGCATGCC AAATGCCTTT GAAAAAGATT CAGTTAGCAA ATGTTGTGTG
    GGGGAGATAA ATGACAGAAC TAAAGATGAA GACATAGCAG GTCCAACCAA TGGTTTGGCT
    CACCAAGAAC AAGAAGCCTG GTTATCCCCA TGCAAAGCTG CTAAAAATAT GATTTTCATA
    CTACCATCAG TTCCAGAAAT TCAGAATCAT CAGCAAAAGG AAGAGCAGCA ATCCATGTCA
    GATGAATTCA ACATTAGTGG TAACATTTCT GAGACTGAGA ATACAAGCTG CAACCAGAAT
    GCATCTCAAG ATGATGAGAG TGCTGAAGTG TTTAATACAG AGACGGTGAA TTCAGTTCTC
    TGGACAAGCA GCGACATTAT GGGAAATGTA GAAGAGACCA CTCCAACAAG GCCTGCTACA
    ACTGAGGATC TTTTTGCTCG TATCCATAGA TCCAAAAAAA AAATTCTTGG ACGCAAAGAT
    TCTGACGATG ATCGTTGCAG AGATCACTCG CCTTCTTCTC CAGTTACCCC AACTGGTAAT
    ATGCATCTAA GTTCCTTCAA GCAGACAGGT TCAATCCAAA GAAGTGTCAG AAAAACTACA
    ACAAGTAGTG ACAGTTTCAA AGCACTGCTG TTAAAAAAGG GAAGCAGGTC TGAAAGTGGC
    TTCCGCATGT CTGCTGCAGA AATGCTGAAA CATACTGATC CAAGGTTTCA GAGATCAAGA
    TCAGAATCAT CTCTGGAGTT TCCAGAAAGC CCCACATTGT CATCACCAAA CAAGAACAAG
    AAGGAGCAAC AGGAATGGGC AAAAAGTGAA GGGCTGATGC CTAGAAGTAT GTCTGGCTCT
    GTTGTCCGGT GCAGCCGCGC CAAGACTCCC CCTTCTGCAT CTAGTAGCAA ATACAATGTC
    CGTAGTCGTC TCCAGAGTAG CCCAATGACT GTTATATGTG AAGGAGAAGG AGAGGGTTCA
    GAAACAGTGG ATAGCAGCTG TTCAAAGGGT CCTCTTTTGT TGAGCACAAA GAGTCTCGAT
    ATCTGTTACA GTGCTGATAA AGAAGTGACC AACCACATAC TGCACATAAA CTTTAACACA
    CTATTAGAAA AGAGCACAGA TGTTGCTAGT GGCTGCACAG GGGAAATAAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32636
    Clone ID Related Accession (Same CDS sequence) XM_012962801.2
    Accession Version XM_012962801.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962801.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCAA
    TGTGACAAGC TTCGGAAGGA TGGCTGTCGC AGTTCACAGT ACTACTCCCA GGGACCAACA
    TTCTCGACAC TCAGACTGGA TTGCACAAGC TATCAGGAAG AGGAAAATAC ACATGAGTCA
    AAGTTTTCTG TTTCATTTAA TGAAGAGGAA CAGATCCTTT CAACCAAAAG GCCCAAGACT
    CCTGTGTTGC TTGAGTTTTC TGAAACGCTC ACTAACTGGA CCAAGTCACT GCCACTGCCT
    ACTCCAGAGG AAAAGATGCG TCAGGAGGCT CAGGCAGTAC AGACAGATGT AATTCCTATA
    AACATAACAG GGGAGAATTT CAACCGCCAG GCCAGCTTTC AGCGGTCACT TATTTATACA
    GACACTGCCG TAAGATGTTC GAAGAAAGTC AAAAGGAGAA AGACTATAAC AGGCGTCCCA
    AACAACTTAC AGAAGGAGCT AGCAGGTACT GGACACTCTG ACCTAAGAAG GAATTCAGTG
    CTCATACCTG ATGATTTTAA TACTTTGGAA CATTGTCACC ACTCAATGCA ATTTCTGGAC
    ACCAGAGATT TTGGCTGCCA AACCAATAAA ATAAAAATAG TGCCACCATC AATTAGAAGA
    ATCAGAGCAC AGAAAGGGCA AGGAATTGGA ATTCTGATGT CCACTTCCTC TGGCAACATG
    TCTACTCTAA GTGACTCTGC AGCTTGTTCT TCATATCTAA ATGGTAACCT GCACTTCCGT
    AGTCTGCCCA GGATTGGTGC AAGAGAATCT CTTCAATCCC TTAACCAAAA AAATTATTAT
    TACAGCAACA TGGAGGACCC TTTTACAACT TTGCCTTGGC AGTTAAACAA ACTGCAAGTG
    GATGATACTA ATGTTCAATT AAGGAGTAAC CCTAGGTCAA CTATTAATCA AAGGCCTGAG
    TCTCAAGAAG TAAAAAATTC TACTAGTGAA ATTGCCTCAA GTCCAGCATG TATGGTCTCG
    CCTCATTCTT CTTATTCAAC AAACATTATT CCAAATGCCA TACTTTCATA TTCCTCAGAA
    GTAATAAGTA TTCACACTCC CCACAGAGGT GGACTTCTGG ACAAGAAACC AAGAGTCCAC
    AGTTCATCTT CTAACAGAAA TGGTATTAGC AGGGACTATA CTTCTGGCAT TAAAGATAAT
    TCCTCACCTG GTAACTGGCG TGAAAACAGT TCATCTAGGA ACTCACAGAT ATCAGATACA
    AAGTCACCTA TCAGTAAAAA AAGAGATGTT CCACACAACG TTCCTTGCAA AATTAACACT
    GGCTTTAACA ACTACTCATT TGACAGTGAC AATCAAAGTG AATTAAGCTA CTCAGATGGT
    CGTCAAAGAA ATGGTAGTAT AACTAGCAGC ATTAATAGTG CTGACAACTG GTTGTATGAA
    TCTAGGCACA ATGACGGTGC TCCATCTAGG AAACTATCTT CTGTAATTTC TGGGTCTCCT
    GTTAGCAATA TGAGCGCCTG CAGTTCTAAG GGGAAGGTAG ATTCCAGCTC TTTGTATTTT
    CTGAACCATG ATGATTTTTG CACATCAATG CATATAGATT CTGGAATTAG TTTGACGGAC
    CAAAGCATTC AGAATCTACA CTATAATATA AATATCTTTG AAACAAAAAA AGCTGCAAAT
    ATAGAAGTGA AAACAGGATA TAGTGACAAA TCACTTCCAC GTAGCATATC TTTAAAGAAA
    TCTAAAAATC CTCCCCCCCC ACCTTCCAGG ACAGACTCAC TTAGAAGGCC ATCTAAGAAA
    GATATTCAGA CCAGTGGGCA GGTGCTCAGT GAAACTCTTA TTGCTACGCT TCAACAGTCC
    TTGCAACTAA ACTTGAAGAG TAAGAGCACT GGGTCCACTT TGCAGTCAAG TCACTGTATG
    GATTATGCAG ATCCCTGCAT CATGTGCTCA TGTAGCCAGA GTACACTTAG CAGCACTAGT
    AGTGGAAAAT CTGTTATTGC TCCAAATATG TACACTCTCT GTACTGTGAC ACCCTCTCAG
    AGTGAAACCA GTAGCATGAA ATCTGAATAT GCGGATCAGT GGAGTTCCTT TATTGACTGT
    TCACAGACTC CAAAAGAGCA ACCCAAATCA TTTGCAAAAC ACAATCTAAG TAATCCTGAT
    GGTCTTAATA ATTACAGTGC ATGTGAGCTG ACCTATGGAA TGAAGACCAT AGCACCACAT
    GGGTACACTG GATCAGTTAA AGTTAGTAAT TCCTCTTCAG AAAAAACATA CAGAGTTACA
    TCACCATCTA GTGGATATTC AAGTCAGTCA AACACTCCCA CCACATTGAC ACCAGTGCCT
    CTTTTCTCAA AGAATATATA TCCAGAAAAT GGAAAATACA AGTTAAAACC AAAAGTACCT
    GAAAGACGAT CTTCATTGGT ATCCTCGGTT TCAGTTTCAT CTTCCTCTAC TTCCCTTTCT
    TCAAATACAT TGGACTCTCT GCAGCAGAAC ATGCAAATTT CAACTGTTAT GTTGTCAGCC
    CATCACACTC TTCCAACCCC ACAACTCACT CCTGACATGC CCCCACCCCC AGCAATGAAT
    GCAACCAACA CAATTTTTGT CGAGTGTTCA CCAAAATGTC CACCTCCTTC CCTTGAATTT
    ACAAGGCTAA CAGCGGATAG CATGGATATG TGGCTTCCCT TCTCTCAAAA CACTGCCATG
    AGTAAACCTA AGGACAGTCT TCCATATCCT TCCTTTCCAC CTTCTCTTTC CATGATGCAC
    AGCATGGTCC CATCACCTGT ACCTCCACTT AACATTGAAA TGCTGAAAAT TGAAACAGAA
    TCATTTCCTC TCAGAAATTC TGAAAGCAGT TATTTTGTAC ATAATGATCA CAGTAAACAT
    GAACTGTTGA TACCAACTGT TCCTATAGTT ACTTCTCATG CACTAGAAAT GGTTCATTTG
    AGATCTGTGA AAAAAGCATC ACAACTGGAA ACTAAAACAT TAAGAGAACA CATGTCAGAA
    TCCCACCTAC AGGATGAGAC ATCCGCCTTA TTGGTACAGC CATGTTTAGT ACCAGCTGCT
    TCTCCAGAAC TAAATTCTAG TGAAAGCCAA ATAGAAAATT TCAGCATTCC TGCCCAGCAA
    GTCTCTGGTT TTAAAAGCAA TGCAGTGAAA CTAACTATTG GTGAAATAGA AGCAGACAAG
    GCATATAAAG AGCACATAGA AAAAAATACA AATTCAGCAT ACAATGGAAA CTCATCAGGG
    CCCAAATTAT CTCCTCATGA GAAACCAAGT ATGTCACAAA GCACACCAAA TTCATCTCTT
    AGCAAGGAGC CGCCTCTTGT TTCAAAGAAG CCTAAACTTG TACTTATTGC CCCAACGCCA
    CAACTTGATT TGGCAGCAGA AAAAATAGCT CAAGTCACTG AAAATGTGAG AAGCATGCCA
    AATGCCTTTG AAAAAGATTC AGTTAGCAAA TGTTGTGTGG GGGAGATAAA TGACAGAACT
    AAAGATGAAG ACATAGCAGG TCCAACCAAT GGTTTGGCTC ACCAAGAACA AGAAGCCTGG
    TTATCCCCAT GCAAAGCTGC TAAAAATATG ATTTTCATAC TACCATCAGT TCCAGAAATT
    CAGAATCATC AGCAAAAGGA AGAGCAGCAA TCCATGTCAG ATGAATTCAA CATTAGTGGT
    AACATTTCTG AGACTGAGAA TACAAGCTGC AACCAGAATG CATCTCAAGA TGATGAGAGT
    GCTGAAGTGT TTAATACAGA GACGGTGAAT TCAGTTCTCT GGACAAGCAG CGACATTATG
    GGAAATGTAG AAGAGACCAC TCCAACAAGG CCTGCTACAA CTGAGGATCT TTTTGCTCGT
    ATCCATAGAT CCAAAAAAAA AATTCTTGGA CGCAAAGATT CTGACGATGA TCGTTGCAGA
    GATCACTCGC CTTCTTCTCC AGTTACCCCA ACTGGTAATA TGCATCTAAG TTCCTTCAAG
    CAGACAGGTT CAATCCAAAG AAGTGTCAGA AAAACTACAA CAAGTAGTGA CAGTTTCAAA
    GCACTGCTGT TAAAAAAGGG AAGCAGGTCT GAAAGTGGCT TCCGCATGTC TGCTGCAGAA
    ATGCTGAAAC ATACTGATCC AAGGTTTCAG AGATCAAGAT CAGAATCATC TCTGGAGTTT
    CCAGAAAGCC CCACATTGTC ATCACCAAAC AAGAACAAGA AGGAGCAACA GGAATGGGCA
    AAAAGTGAAG GGCTGATGCC TAGAAGTATG TCTGGCTCTG TTGTCCGGTG CAGCCGCGCC
    AAGACTCCCC CTTCTGCATC TAGTAGCAAA TACAATGTCC GTAGTCGTCT CCAGAGTAGC
    CCAATGACTG TTATATGTGA AGGAGAAGGA GAGGGTTCAG AAACAGTGGA TAGCAGCTGT
    TCAAAGGGTC CTCTTTTGTT GAGCACAAAG AGTCTCGATA TCTGTTACAG TGCTGATAAA
    GAAGTGACCA ACCACATACT GCACATAAAC TTTAACACAC TATTAGAAAA GAGCACAGAT
    GTTGCTAGTG GCTGCACAGG GGAAATAATT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818255.1
    CDS636..5408
    Translation

    Target ORF information:

    RefSeq Version XM_012962801.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962801.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCAA
    TGTGACAAGC TTCGGAAGGA TGGCTGTCGC AGTTCACAGT ACTACTCCCA GGGACCAACA
    TTCTCGACAC TCAGACTGGA TTGCACAAGC TATCAGGAAG AGGAAAATAC ACATGAGTCA
    AAGTTTTCTG TTTCATTTAA TGAAGAGGAA CAGATCCTTT CAACCAAAAG GCCCAAGACT
    CCTGTGTTGC TTGAGTTTTC TGAAACGCTC ACTAACTGGA CCAAGTCACT GCCACTGCCT
    ACTCCAGAGG AAAAGATGCG TCAGGAGGCT CAGGCAGTAC AGACAGATGT AATTCCTATA
    AACATAACAG GGGAGAATTT CAACCGCCAG GCCAGCTTTC AGCGGTCACT TATTTATACA
    GACACTGCCG TAAGATGTTC GAAGAAAGTC AAAAGGAGAA AGACTATAAC AGGCGTCCCA
    AACAACTTAC AGAAGGAGCT AGCAGGTACT GGACACTCTG ACCTAAGAAG GAATTCAGTG
    CTCATACCTG ATGATTTTAA TACTTTGGAA CATTGTCACC ACTCAATGCA ATTTCTGGAC
    ACCAGAGATT TTGGCTGCCA AACCAATAAA ATAAAAATAG TGCCACCATC AATTAGAAGA
    ATCAGAGCAC AGAAAGGGCA AGGAATTGGA ATTCTGATGT CCACTTCCTC TGGCAACATG
    TCTACTCTAA GTGACTCTGC AGCTTGTTCT TCATATCTAA ATGGTAACCT GCACTTCCGT
    AGTCTGCCCA GGATTGGTGC AAGAGAATCT CTTCAATCCC TTAACCAAAA AAATTATTAT
    TACAGCAACA TGGAGGACCC TTTTACAACT TTGCCTTGGC AGTTAAACAA ACTGCAAGTG
    GATGATACTA ATGTTCAATT AAGGAGTAAC CCTAGGTCAA CTATTAATCA AAGGCCTGAG
    TCTCAAGAAG TAAAAAATTC TACTAGTGAA ATTGCCTCAA GTCCAGCATG TATGGTCTCG
    CCTCATTCTT CTTATTCAAC AAACATTATT CCAAATGCCA TACTTTCATA TTCCTCAGAA
    GTAATAAGTA TTCACACTCC CCACAGAGGT GGACTTCTGG ACAAGAAACC AAGAGTCCAC
    AGTTCATCTT CTAACAGAAA TGGTATTAGC AGGGACTATA CTTCTGGCAT TAAAGATAAT
    TCCTCACCTG GTAACTGGCG TGAAAACAGT TCATCTAGGA ACTCACAGAT ATCAGATACA
    AAGTCACCTA TCAGTAAAAA AAGAGATGTT CCACACAACG TTCCTTGCAA AATTAACACT
    GGCTTTAACA ACTACTCATT TGACAGTGAC AATCAAAGTG AATTAAGCTA CTCAGATGGT
    CGTCAAAGAA ATGGTAGTAT AACTAGCAGC ATTAATAGTG CTGACAACTG GTTGTATGAA
    TCTAGGCACA ATGACGGTGC TCCATCTAGG AAACTATCTT CTGTAATTTC TGGGTCTCCT
    GTTAGCAATA TGAGCGCCTG CAGTTCTAAG GGGAAGGTAG ATTCCAGCTC TTTGTATTTT
    CTGAACCATG ATGATTTTTG CACATCAATG CATATAGATT CTGGAATTAG TTTGACGGAC
    CAAAGCATTC AGAATCTACA CTATAATATA AATATCTTTG AAACAAAAAA AGCTGCAAAT
    ATAGAAGTGA AAACAGGATA TAGTGACAAA TCACTTCCAC GTAGCATATC TTTAAAGAAA
    TCTAAAAATC CTCCCCCCCC ACCTTCCAGG ACAGACTCAC TTAGAAGGCC ATCTAAGAAA
    GATATTCAGA CCAGTGGGCA GGTGCTCAGT GAAACTCTTA TTGCTACGCT TCAACAGTCC
    TTGCAACTAA ACTTGAAGAG TAAGAGCACT GGGTCCACTT TGCAGTCAAG TCACTGTATG
    GATTATGCAG ATCCCTGCAT CATGTGCTCA TGTAGCCAGA GTACACTTAG CAGCACTAGT
    AGTGGAAAAT CTGTTATTGC TCCAAATATG TACACTCTCT GTACTGTGAC ACCCTCTCAG
    AGTGAAACCA GTAGCATGAA ATCTGAATAT GCGGATCAGT GGAGTTCCTT TATTGACTGT
    TCACAGACTC CAAAAGAGCA ACCCAAATCA TTTGCAAAAC ACAATCTAAG TAATCCTGAT
    GGTCTTAATA ATTACAGTGC ATGTGAGCTG ACCTATGGAA TGAAGACCAT AGCACCACAT
    GGGTACACTG GATCAGTTAA AGTTAGTAAT TCCTCTTCAG AAAAAACATA CAGAGTTACA
    TCACCATCTA GTGGATATTC AAGTCAGTCA AACACTCCCA CCACATTGAC ACCAGTGCCT
    CTTTTCTCAA AGAATATATA TCCAGAAAAT GGAAAATACA AGTTAAAACC AAAAGTACCT
    GAAAGACGAT CTTCATTGGT ATCCTCGGTT TCAGTTTCAT CTTCCTCTAC TTCCCTTTCT
    TCAAATACAT TGGACTCTCT GCAGCAGAAC ATGCAAATTT CAACTGTTAT GTTGTCAGCC
    CATCACACTC TTCCAACCCC ACAACTCACT CCTGACATGC CCCCACCCCC AGCAATGAAT
    GCAACCAACA CAATTTTTGT CGAGTGTTCA CCAAAATGTC CACCTCCTTC CCTTGAATTT
    ACAAGGCTAA CAGCGGATAG CATGGATATG TGGCTTCCCT TCTCTCAAAA CACTGCCATG
    AGTAAACCTA AGGACAGTCT TCCATATCCT TCCTTTCCAC CTTCTCTTTC CATGATGCAC
    AGCATGGTCC CATCACCTGT ACCTCCACTT AACATTGAAA TGCTGAAAAT TGAAACAGAA
    TCATTTCCTC TCAGAAATTC TGAAAGCAGT TATTTTGTAC ATAATGATCA CAGTAAACAT
    GAACTGTTGA TACCAACTGT TCCTATAGTT ACTTCTCATG CACTAGAAAT GGTTCATTTG
    AGATCTGTGA AAAAAGCATC ACAACTGGAA ACTAAAACAT TAAGAGAACA CATGTCAGAA
    TCCCACCTAC AGGATGAGAC ATCCGCCTTA TTGGTACAGC CATGTTTAGT ACCAGCTGCT
    TCTCCAGAAC TAAATTCTAG TGAAAGCCAA ATAGAAAATT TCAGCATTCC TGCCCAGCAA
    GTCTCTGGTT TTAAAAGCAA TGCAGTGAAA CTAACTATTG GTGAAATAGA AGCAGACAAG
    GCATATAAAG AGCACATAGA AAAAAATACA AATTCAGCAT ACAATGGAAA CTCATCAGGG
    CCCAAATTAT CTCCTCATGA GAAACCAAGT ATGTCACAAA GCACACCAAA TTCATCTCTT
    AGCAAGGAGC CGCCTCTTGT TTCAAAGAAG CCTAAACTTG TACTTATTGC CCCAACGCCA
    CAACTTGATT TGGCAGCAGA AAAAATAGCT CAAGTCACTG AAAATGTGAG AAGCATGCCA
    AATGCCTTTG AAAAAGATTC AGTTAGCAAA TGTTGTGTGG GGGAGATAAA TGACAGAACT
    AAAGATGAAG ACATAGCAGG TCCAACCAAT GGTTTGGCTC ACCAAGAACA AGAAGCCTGG
    TTATCCCCAT GCAAAGCTGC TAAAAATATG ATTTTCATAC TACCATCAGT TCCAGAAATT
    CAGAATCATC AGCAAAAGGA AGAGCAGCAA TCCATGTCAG ATGAATTCAA CATTAGTGGT
    AACATTTCTG AGACTGAGAA TACAAGCTGC AACCAGAATG CATCTCAAGA TGATGAGAGT
    GCTGAAGTGT TTAATACAGA GACGGTGAAT TCAGTTCTCT GGACAAGCAG CGACATTATG
    GGAAATGTAG AAGAGACCAC TCCAACAAGG CCTGCTACAA CTGAGGATCT TTTTGCTCGT
    ATCCATAGAT CCAAAAAAAA AATTCTTGGA CGCAAAGATT CTGACGATGA TCGTTGCAGA
    GATCACTCGC CTTCTTCTCC AGTTACCCCA ACTGGTAATA TGCATCTAAG TTCCTTCAAG
    CAGACAGGTT CAATCCAAAG AAGTGTCAGA AAAACTACAA CAAGTAGTGA CAGTTTCAAA
    GCACTGCTGT TAAAAAAGGG AAGCAGGTCT GAAAGTGGCT TCCGCATGTC TGCTGCAGAA
    ATGCTGAAAC ATACTGATCC AAGGTTTCAG AGATCAAGAT CAGAATCATC TCTGGAGTTT
    CCAGAAAGCC CCACATTGTC ATCACCAAAC AAGAACAAGA AGGAGCAACA GGAATGGGCA
    AAAAGTGAAG GGCTGATGCC TAGAAGTATG TCTGGCTCTG TTGTCCGGTG CAGCCGCGCC
    AAGACTCCCC CTTCTGCATC TAGTAGCAAA TACAATGTCC GTAGTCGTCT CCAGAGTAGC
    CCAATGACTG TTATATGTGA AGGAGAAGGA GAGGGTTCAG AAACAGTGGA TAGCAGCTGT
    TCAAAGGGTC CTCTTTTGTT GAGCACAAAG AGTCTCGATA TCTGTTACAG TGCTGATAAA
    GAAGTGACCA ACCACATACT GCACATAAAC TTTAACACAC TATTAGAAAA GAGCACAGAT
    GTTGCTAGTG GCTGCACAGG GGAAATAATT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32637
    Clone ID Related Accession (Same CDS sequence) XM_012962802.2 , XM_012962802.3
    Accession Version XM_012962802.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTTATAT TCGTTAATGC AATGCTGAAA TCATTTATTA GTATGTTCAA GAAAAAAACG 
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT
    GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC
    CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC
    AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG
    TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT
    CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA
    AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT
    ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC
    AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA
    GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA
    AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA
    AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA
    ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818256.1
    CDS585..5270
    Translation

    Target ORF information:

    RefSeq Version XM_012962802.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962802.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTTATAT TCGTTAATGC AATGCTGAAA TCATTTATTA GTATGTTCAA GAAAAAAACG 
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT
    GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC
    CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC
    AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG
    TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT
    CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA
    AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT
    ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC
    AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA
    GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA
    AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA
    AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA
    ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32638
    Clone ID Related Accession (Same CDS sequence) XM_012962803.2
    Accession Version XM_012962803.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGCAACA AGCCGGGGGG CTGTCGTGTC GGCCCTGCAA CGCGATCGTC GCAGCAGACG 
    CGATCACATC GCGTCTGCTG CTTCCCCAGT GCCGGCCCAA TGCCTAGGGG GACTTCCTGC
    TCCAGGACTG CAGCACCGAG GACCTGGCAA GCTTCAGGAC AGAATGTGAC AAGCTTCGGA
    AGGATGGCTG TCGCAGTTCA CAGTACTACT CCCAGGGACC AACATTCTCG ACACTCAGAC
    TGGATTGCAC AAGCTATCAG GAAGAGGAAA ATACACATGA GTCAAAGTGC AAACCCACCA
    TGGACCCGCG GCCAGCCTTT TTCTGTTTCA TTTAATGAAG AGGAACAGAT CCTTTCAACC
    AAAAGGCCCA AGACTCCTGT GTTGCTTGAG TTTTCTGAAA CGCTCACTAA CTGGACCAAG
    TCACTGCCAC TGCCTACTCC AGAGGAAAAG ATGCGTCAGG AGGCTCAGGC AGTACAGACA
    GATGTAATTC CTATAAACAT AACAGGGGAG AATTTCAACC GCCAGGCCAG CTTTCAGCGG
    TCACTTATTT ATACAGACAC TGCCGTAAGA TGTTCGAAGA AAGTCAAAAG GAGAAAGACT
    ATAACAGGCG TCCCAAACAA CTTACAGAAG GAGCTAGCAG GTACTGGACA CTCTGACCTA
    AGAAGGAATT CAGTGCTCAT ACCTGATGAT TTTAATACTT TGGAACATTG TCACCACTCA
    ATGCAATTTC TGGACACCAG AGATTTTGGC TGCCAAACCA ATAAAATAAA AATAGTGCCA
    CCATCAATTA GAAGAATCAG AGCACAGAAA GGGCAAGGAA TTGGAATTCT GATGTCCACT
    TCCTCTGGCA ACATGTCTAC TCTAAGTGAC TCTGCAGCTT GTTCTTCATA TCTAAATGGT
    AACCTGCACT TCCGTAGTCT GCCCAGGATT GGTGCAAGAG AATCTCTTCA ATCCCTTAAC
    CAAAAAAATT ATTATTACAG CAACATGGAG GACCCTTTTA CAACTTTGCC TTGGCAGTTA
    AACAAACTGC AAGTGGATGA TACTAATGTT CAATTAAGGA GTAACCCTAG GTCAACTATT
    AATCAAAGGC CTGAGTCTCA AGAAGTAAAA AATTCTACTA GTGAAATTGC CTCAAGTCCA
    GCATGTATGG TCTCGCCTCA TTCTTCTTAT TCAACAAACA TTATTCCAAA TGCCATACTT
    TCATATTCCT CAGAAGTAAT AAGTATTCAC ACTCCCCACA GAGGTGGACT TCTGGACAAG
    AAACCAAGAG TCCACAGTTC ATCTTCTAAC AGAAATGGTA TTAGCAGGGA CTATACTTCT
    GGCATTAAAG ATAATTCCTC ACCTGGTAAC TGGCGTGAAA ACAGTTCATC TAGGAACTCA
    CAGATATCAG ATACAAAGTC ACCTATCAGT AAAAAAAGAG ATGTTCCACA CAACGTTCCT
    TGCAAAATTA ACACTGGCTT TAACAACTAC TCATTTGACA GTGACAATCA AAGTGAATTA
    AGCTACTCAG ATGGTCGTCA AAGAAATGGT AGTATAACTA GCAGCATTAA TAGTGCTGAC
    AACTGGTTGT ATGAATCTAG GCACAATGAC GGTGCTCCAT CTAGGAAACT ATCTTCTGTA
    ATTTCTGGGT CTCCTGTTAG CAATATGAGC GCCTGCAGTT CTAAGGGGAA GGTAGATTCC
    AGCTCTTTGT ATTTTCTGAA CCATGATGAT TTTTGCACAT CAATGCATAT AGATTCTGGA
    ATTAGTTTGA CGGACCAAAG CATTCAGAAT CTACACTATA ATATAAATAT CTTTGAAACA
    AAAAAAGCTG CAAATATAGA AGTGAAAACA GGATATAGTG ACAAATCACT TCCACGTAGC
    ATATCTTTAA AGAAATCTAA AAATCCTCCC CCCCCACCTT CCAGGACAGA CTCACTTAGA
    AGGCCATCTA AGAAAGATAT TCAGACCAGT GGGCAGGTGC TCAGTGAAAC TCTTATTGCT
    ACGCTTCAAC AGTCCTTGCA ACTAAACTTG AAGAGTAAGA GCACTGGGTC CACTTTGCAG
    TCAAGTCACT GTATGGATTA TGCAGATCCC TGCATCATGT GCTCATGTAG CCAGAGTACA
    CTTAGCAGCA CTAGTAGTGG AAAATCTGTT ATTGCTCCAA ATATGTACAC TCTCTGTACT
    GTGACACCCT CTCAGAGTGA AACCAGTAGC ATGAAATCTG AATATGCGGA TCAGTGGAGT
    TCCTTTATTG ACTGTTCACA GACTCCAAAA GAGCAACCCA AATCATTTGC AAAACACAAT
    CTAAGTAATC CTGATGGTCT TAATAATTAC AGTGCATGTG AGCTGACCTA TGGAATGAAG
    ACCATAGCAC CACATGGGTA CACTGGATCA GTTAAAGTTA GTAATTCCTC TTCAGAAAAA
    ACATACAGAG TTACATCACC ATCTAGTGGA TATTCAAGTC AGTCAAACAC TCCCACCACA
    TTGACACCAG TGCCTCTTTT CTCAAAGAAT ATATATCCAG AAAATGGAAA ATACAAGTTA
    AAACCAAAAG TACCTGAAAG ACGATCTTCA TTGGTATCCT CGGTTTCAGT TTCATCTTCC
    TCTACTTCCC TTTCTTCAAA TACATTGGAC TCTCTGCAGC AGAACATGCA AATTTCAACT
    GTTATGTTGT CAGCCCATCA CACTCTTCCA ACCCCACAAC TCACTCCTGA CATGCCCCCA
    CCCCCAGCAA TGAATGCAAC CAACACAATT TTTGTCGAGT GTTCACCAAA ATGTCCACCT
    CCTTCCCTTG AATTTACAAG GCTAACAGCG GATAGCATGG ATATGTGGCT TCCCTTCTCT
    CAAAACACTG CCATGAGTAA ACCTAAGGAC AGTCTTCCAT ATCCTTCCTT TCCACCTTCT
    CTTTCCATGA TGCACAGCAT GGTCCCATCA CCTGTACCTC CACTTAACAT TGAAATGCTG
    AAAATTGAAA CAGAATCATT TCCTCTCAGA AATTCTGAAA GCAGTTATTT TGTACATAAT
    GATCACAGTA AACATGAACT GTTGATACCA ACTGTTCCTA TAGTTACTTC TCATGCACTA
    GAAATGGTTC ATTTGAGATC TGTGAAAAAA GCATCACAAC TGGAAACTAA AACATTAAGA
    GAACACATGT CAGAATCCCA CCTACAGGAT GAGACATCCG CCTTATTGGT ACAGCCATGT
    TTAGTACCAG CTGCTTCTCC AGAACTAAAT TCTAGTGAAA GCCAAATAGA AAATTTCAGC
    ATTCCTGCCC AGCAAGTCTC TGGTTTTAAA AGCAATGCAG TGAAACTAAC TATTGGTGAA
    ATAGAAGCAG ACAAGGCATA TAAAGAGCAC ATAGAAAAAA ATACAAATTC AGCATACAAT
    GGAAACTCAT CAGGGCCCAA ATTATCTCCT CATGAGAAAC CAAGTATGTC ACAAAGCACA
    CCAAATTCAT CTCTTAGCAA GGAGCCGCCT CTTGTTTCAA AGAAGCCTAA ACTTGTACTT
    ATTGCCCCAA CGCCACAACT TGATTTGGCA GCAGAAAAAA TAGCTCAAGT CACTGAAAAT
    GTGAGAAGCA TGCCAAATGC CTTTGAAAAA GATTCAGTTA GCAAATGTTG TGTGGGGGAG
    ATAAATGACA GAACTAAAGA TGAAGACATA GCAGGTCCAA CCAATGGTTT GGCTCACCAA
    GAACAAGAAG CCTGGTTATC CCCATGCAAA GCTGCTAAAA ATATGATTTT CATACTACCA
    TCAGTTCCAG AAATTCAGAA TCATCAGCAA AAGGAAGAGC AGCAATCCAT GTCAGATGAA
    TTCAACATTA GTGGTAACAT TTCTGAGACT GAGAATACAA GCTGCAACCA GAATGCATCT
    CAAGATGATG AGAGTGCTGA AGTGTTTAAT ACAGAGACGG TGAATTCAGT TCTCTGGACA
    AGCAGCGACA TTATGGGAAA TGTAGAAGAG ACCACTCCAA CAAGGCCTGC TACAACTGAG
    GATCTTTTTG CTCGTATCCA TAGATCCAAA AAAAAAATTC TTGGACGCAA AGATTCTGAC
    GATGATCGTT GCAGAGATCA CTCGCCTTCT TCTCCAGTTA CCCCAACTGG TAATATGCAT
    CTAAGTTCCT TCAAGCAGAC AGGTTCAATC CAAAGAAGTG TCAGAAAAAC TACAACAAGT
    AGTGACAGTT TCAAAGCACT GCTGTTAAAA AAGGGAAGCA GGTCTGAAAG TGGCTTCCGC
    ATGTCTGCTG CAGAAATGCT GAAACATACT GATCCAAGGT TTCAGAGATC AAGATCAGAA
    TCATCTCTGG AGTTTCCAGA AAGCCCCACA TTGTCATCAC CAAACAAGAA CAAGAAGGAG
    CAACAGGAAT GGGCAAAAAG TGAAGGGCTG ATGCCTAGAA GTATGTCTGG CTCTGTTGTC
    CGGTGCAGCC GCGCCAAGAC TCCCCCTTCT GCATCTAGTA GCAAATACAA TGTCCGTAGT
    CGTCTCCAGA GTAGCCCAAT GACTGTTATA TGTGAAGGAG AAGGAGAGGG TTCAGAAACA
    GTGGATAGCA GCTGTTCAAA GGGTCCTCTT TTGTTGAGCA CAAAGAGTCT CGATATCTGT
    TACAGTGCTG ATAAAGAAGT GACCAACCAC ATACTGCACA TAAACTTTAA CACACTATTA
    GAAAAGAGCA CAGATGTTGC TAGTGGCTGC ACAGGGGAAA TAATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818257.1
    CDS28..4695
    Translation

    Target ORF information:

    RefSeq Version XM_012962803.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962803.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGCAACA AGCCGGGGGG CTGTCGTGTC GGCCCTGCAA CGCGATCGTC GCAGCAGACG 
    CGATCACATC GCGTCTGCTG CTTCCCCAGT GCCGGCCCAA TGCCTAGGGG GACTTCCTGC
    TCCAGGACTG CAGCACCGAG GACCTGGCAA GCTTCAGGAC AGAATGTGAC AAGCTTCGGA
    AGGATGGCTG TCGCAGTTCA CAGTACTACT CCCAGGGACC AACATTCTCG ACACTCAGAC
    TGGATTGCAC AAGCTATCAG GAAGAGGAAA ATACACATGA GTCAAAGTGC AAACCCACCA
    TGGACCCGCG GCCAGCCTTT TTCTGTTTCA TTTAATGAAG AGGAACAGAT CCTTTCAACC
    AAAAGGCCCA AGACTCCTGT GTTGCTTGAG TTTTCTGAAA CGCTCACTAA CTGGACCAAG
    TCACTGCCAC TGCCTACTCC AGAGGAAAAG ATGCGTCAGG AGGCTCAGGC AGTACAGACA
    GATGTAATTC CTATAAACAT AACAGGGGAG AATTTCAACC GCCAGGCCAG CTTTCAGCGG
    TCACTTATTT ATACAGACAC TGCCGTAAGA TGTTCGAAGA AAGTCAAAAG GAGAAAGACT
    ATAACAGGCG TCCCAAACAA CTTACAGAAG GAGCTAGCAG GTACTGGACA CTCTGACCTA
    AGAAGGAATT CAGTGCTCAT ACCTGATGAT TTTAATACTT TGGAACATTG TCACCACTCA
    ATGCAATTTC TGGACACCAG AGATTTTGGC TGCCAAACCA ATAAAATAAA AATAGTGCCA
    CCATCAATTA GAAGAATCAG AGCACAGAAA GGGCAAGGAA TTGGAATTCT GATGTCCACT
    TCCTCTGGCA ACATGTCTAC TCTAAGTGAC TCTGCAGCTT GTTCTTCATA TCTAAATGGT
    AACCTGCACT TCCGTAGTCT GCCCAGGATT GGTGCAAGAG AATCTCTTCA ATCCCTTAAC
    CAAAAAAATT ATTATTACAG CAACATGGAG GACCCTTTTA CAACTTTGCC TTGGCAGTTA
    AACAAACTGC AAGTGGATGA TACTAATGTT CAATTAAGGA GTAACCCTAG GTCAACTATT
    AATCAAAGGC CTGAGTCTCA AGAAGTAAAA AATTCTACTA GTGAAATTGC CTCAAGTCCA
    GCATGTATGG TCTCGCCTCA TTCTTCTTAT TCAACAAACA TTATTCCAAA TGCCATACTT
    TCATATTCCT CAGAAGTAAT AAGTATTCAC ACTCCCCACA GAGGTGGACT TCTGGACAAG
    AAACCAAGAG TCCACAGTTC ATCTTCTAAC AGAAATGGTA TTAGCAGGGA CTATACTTCT
    GGCATTAAAG ATAATTCCTC ACCTGGTAAC TGGCGTGAAA ACAGTTCATC TAGGAACTCA
    CAGATATCAG ATACAAAGTC ACCTATCAGT AAAAAAAGAG ATGTTCCACA CAACGTTCCT
    TGCAAAATTA ACACTGGCTT TAACAACTAC TCATTTGACA GTGACAATCA AAGTGAATTA
    AGCTACTCAG ATGGTCGTCA AAGAAATGGT AGTATAACTA GCAGCATTAA TAGTGCTGAC
    AACTGGTTGT ATGAATCTAG GCACAATGAC GGTGCTCCAT CTAGGAAACT ATCTTCTGTA
    ATTTCTGGGT CTCCTGTTAG CAATATGAGC GCCTGCAGTT CTAAGGGGAA GGTAGATTCC
    AGCTCTTTGT ATTTTCTGAA CCATGATGAT TTTTGCACAT CAATGCATAT AGATTCTGGA
    ATTAGTTTGA CGGACCAAAG CATTCAGAAT CTACACTATA ATATAAATAT CTTTGAAACA
    AAAAAAGCTG CAAATATAGA AGTGAAAACA GGATATAGTG ACAAATCACT TCCACGTAGC
    ATATCTTTAA AGAAATCTAA AAATCCTCCC CCCCCACCTT CCAGGACAGA CTCACTTAGA
    AGGCCATCTA AGAAAGATAT TCAGACCAGT GGGCAGGTGC TCAGTGAAAC TCTTATTGCT
    ACGCTTCAAC AGTCCTTGCA ACTAAACTTG AAGAGTAAGA GCACTGGGTC CACTTTGCAG
    TCAAGTCACT GTATGGATTA TGCAGATCCC TGCATCATGT GCTCATGTAG CCAGAGTACA
    CTTAGCAGCA CTAGTAGTGG AAAATCTGTT ATTGCTCCAA ATATGTACAC TCTCTGTACT
    GTGACACCCT CTCAGAGTGA AACCAGTAGC ATGAAATCTG AATATGCGGA TCAGTGGAGT
    TCCTTTATTG ACTGTTCACA GACTCCAAAA GAGCAACCCA AATCATTTGC AAAACACAAT
    CTAAGTAATC CTGATGGTCT TAATAATTAC AGTGCATGTG AGCTGACCTA TGGAATGAAG
    ACCATAGCAC CACATGGGTA CACTGGATCA GTTAAAGTTA GTAATTCCTC TTCAGAAAAA
    ACATACAGAG TTACATCACC ATCTAGTGGA TATTCAAGTC AGTCAAACAC TCCCACCACA
    TTGACACCAG TGCCTCTTTT CTCAAAGAAT ATATATCCAG AAAATGGAAA ATACAAGTTA
    AAACCAAAAG TACCTGAAAG ACGATCTTCA TTGGTATCCT CGGTTTCAGT TTCATCTTCC
    TCTACTTCCC TTTCTTCAAA TACATTGGAC TCTCTGCAGC AGAACATGCA AATTTCAACT
    GTTATGTTGT CAGCCCATCA CACTCTTCCA ACCCCACAAC TCACTCCTGA CATGCCCCCA
    CCCCCAGCAA TGAATGCAAC CAACACAATT TTTGTCGAGT GTTCACCAAA ATGTCCACCT
    CCTTCCCTTG AATTTACAAG GCTAACAGCG GATAGCATGG ATATGTGGCT TCCCTTCTCT
    CAAAACACTG CCATGAGTAA ACCTAAGGAC AGTCTTCCAT ATCCTTCCTT TCCACCTTCT
    CTTTCCATGA TGCACAGCAT GGTCCCATCA CCTGTACCTC CACTTAACAT TGAAATGCTG
    AAAATTGAAA CAGAATCATT TCCTCTCAGA AATTCTGAAA GCAGTTATTT TGTACATAAT
    GATCACAGTA AACATGAACT GTTGATACCA ACTGTTCCTA TAGTTACTTC TCATGCACTA
    GAAATGGTTC ATTTGAGATC TGTGAAAAAA GCATCACAAC TGGAAACTAA AACATTAAGA
    GAACACATGT CAGAATCCCA CCTACAGGAT GAGACATCCG CCTTATTGGT ACAGCCATGT
    TTAGTACCAG CTGCTTCTCC AGAACTAAAT TCTAGTGAAA GCCAAATAGA AAATTTCAGC
    ATTCCTGCCC AGCAAGTCTC TGGTTTTAAA AGCAATGCAG TGAAACTAAC TATTGGTGAA
    ATAGAAGCAG ACAAGGCATA TAAAGAGCAC ATAGAAAAAA ATACAAATTC AGCATACAAT
    GGAAACTCAT CAGGGCCCAA ATTATCTCCT CATGAGAAAC CAAGTATGTC ACAAAGCACA
    CCAAATTCAT CTCTTAGCAA GGAGCCGCCT CTTGTTTCAA AGAAGCCTAA ACTTGTACTT
    ATTGCCCCAA CGCCACAACT TGATTTGGCA GCAGAAAAAA TAGCTCAAGT CACTGAAAAT
    GTGAGAAGCA TGCCAAATGC CTTTGAAAAA GATTCAGTTA GCAAATGTTG TGTGGGGGAG
    ATAAATGACA GAACTAAAGA TGAAGACATA GCAGGTCCAA CCAATGGTTT GGCTCACCAA
    GAACAAGAAG CCTGGTTATC CCCATGCAAA GCTGCTAAAA ATATGATTTT CATACTACCA
    TCAGTTCCAG AAATTCAGAA TCATCAGCAA AAGGAAGAGC AGCAATCCAT GTCAGATGAA
    TTCAACATTA GTGGTAACAT TTCTGAGACT GAGAATACAA GCTGCAACCA GAATGCATCT
    CAAGATGATG AGAGTGCTGA AGTGTTTAAT ACAGAGACGG TGAATTCAGT TCTCTGGACA
    AGCAGCGACA TTATGGGAAA TGTAGAAGAG ACCACTCCAA CAAGGCCTGC TACAACTGAG
    GATCTTTTTG CTCGTATCCA TAGATCCAAA AAAAAAATTC TTGGACGCAA AGATTCTGAC
    GATGATCGTT GCAGAGATCA CTCGCCTTCT TCTCCAGTTA CCCCAACTGG TAATATGCAT
    CTAAGTTCCT TCAAGCAGAC AGGTTCAATC CAAAGAAGTG TCAGAAAAAC TACAACAAGT
    AGTGACAGTT TCAAAGCACT GCTGTTAAAA AAGGGAAGCA GGTCTGAAAG TGGCTTCCGC
    ATGTCTGCTG CAGAAATGCT GAAACATACT GATCCAAGGT TTCAGAGATC AAGATCAGAA
    TCATCTCTGG AGTTTCCAGA AAGCCCCACA TTGTCATCAC CAAACAAGAA CAAGAAGGAG
    CAACAGGAAT GGGCAAAAAG TGAAGGGCTG ATGCCTAGAA GTATGTCTGG CTCTGTTGTC
    CGGTGCAGCC GCGCCAAGAC TCCCCCTTCT GCATCTAGTA GCAAATACAA TGTCCGTAGT
    CGTCTCCAGA GTAGCCCAAT GACTGTTATA TGTGAAGGAG AAGGAGAGGG TTCAGAAACA
    GTGGATAGCA GCTGTTCAAA GGGTCCTCTT TTGTTGAGCA CAAAGAGTCT CGATATCTGT
    TACAGTGCTG ATAAAGAAGT GACCAACCAC ATACTGCACA TAAACTTTAA CACACTATTA
    GAAAAGAGCA CAGATGTTGC TAGTGGCTGC ACAGGGGAAA TAATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32639
    Clone ID Related Accession (Same CDS sequence) XM_012962805.2 , XM_012962804.2
    Accession Version XM_012962805.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGTCG CAGTTCACAG TACTACTCCC AGGGACCAAC ATTCTCGACA CTCAGACTGG 
    ATTGCACAAG CTATCAGGAA GAGGAAAATA CACATGAGTC AAAGTGCAAA CCCACCATGG
    ACCCGCGGCC AGCCTTTTTC TGTTTCATTT AATGAAGAGG AACAGATCCT TTCAACCAAA
    AGGCCCAAGA CTCCTGTGTT GCTTGAGTTT TCTGAAACGC TCACTAACTG GACCAAGTCA
    CTGCCACTGC CTACTCCAGA GGAAAAGATG CGTCAGGAGG CTCAGGCAGT ACAGACAGAT
    GTAATTCCTA TAAACATAAC AGGGGAGAAT TTCAACCGCC AGGCCAGCTT TCAGCGGTCA
    CTTATTTATA CAGACACTGC CGTAAGATGT TCGAAGAAAG TCAAAAGGAG AAAGACTATA
    ACAGGCGTCC CAAACAACTT ACAGAAGGAG CTAGCAGGTA CTGGACACTC TGACCTAAGA
    AGGAATTCAG TGCTCATACC TGATGATTTT AATACTTTGG AACATTGTCA CCACTCAATG
    CAATTTCTGG ACACCAGAGA TTTTGGCTGC CAAACCAATA AAATAAAAAT AGTGCCACCA
    TCAATTAGAA GAATCAGAGC ACAGAAAGGG CAAGGAATTG GAATTCTGAT GTCCACTTCC
    TCTGGCAACA TGTCTACTCT AAGTGACTCT GCAGCTTGTT CTTCATATCT AAATGGTAAC
    CTGCACTTCC GTAGTCTGCC CAGGATTGGT GCAAGAGAAT CTCTTCAATC CCTTAACCAA
    AAAAATTATT ATTACAGCAA CATGGAGGAC CCTTTTACAA CTTTGCCTTG GCAGTTAAAC
    AAACTGCAAG TGGATGATAC TAATGTTCAA TTAAGGAGTA ACCCTAGGTC AACTATTAAT
    CAAAGGCCTG AGTCTCAAGA AGTAAAAAAT TCTACTAGTG AAATTGCCTC AAGTCCAGCA
    TGTATGGTCT CGCCTCATTC TTCTTATTCA ACAAACATTA TTCCAAATGC CATACTTTCA
    TATTCCTCAG AAGTAATAAG TATTCACACT CCCCACAGAG GTGGACTTCT GGACAAGAAA
    CCAAGAGTCC ACAGTTCATC TTCTAACAGA AATGGTATTA GCAGGGACTA TACTTCTGGC
    ATTAAAGATA ATTCCTCACC TGGTAACTGG CGTGAAAACA GTTCATCTAG GAACTCACAG
    ATATCAGATA CAAAGTCACC TATCAGTAAA AAAAGAGATG TTCCACACAA CGTTCCTTGC
    AAAATTAACA CTGGCTTTAA CAACTACTCA TTTGACAGTG ACAATCAAAG TGAATTAAGC
    TACTCAGATG GTCGTCAAAG AAATGGTAGT ATAACTAGCA GCATTAATAG TGCTGACAAC
    TGGTTGTATG AATCTAGGCA CAATGACGGT GCTCCATCTA GGAAACTATC TTCTGTAATT
    TCTGGGTCTC CTGTTAGCAA TATGAGCGCC TGCAGTTCTA AGGGGAAGGT AGATTCCAGC
    TCTTTGTATT TTCTGAACCA TGATGATTTT TGCACATCAA TGCATATAGA TTCTGGAATT
    AGTTTGACGG ACCAAAGCAT TCAGAATCTA CACTATAATA TAAATATCTT TGAAACAAAA
    AAAGCTGCAA ATATAGAAGT GAAAACAGGA TATAGTGACA AATCACTTCC ACGTAGCATA
    TCTTTAAAGA AATCTAAAAA TCCTCCCCCC CCACCTTCCA GGACAGACTC ACTTAGAAGG
    CCATCTAAGA AAGATATTCA GACCAGTGGG CAGGTGCTCA GTGAAACTCT TATTGCTACG
    CTTCAACAGT CCTTGCAACT AAACTTGAAG AGTAAGAGCA CTGGGTCCAC TTTGCAGTCA
    AGTCACTGTA TGGATTATGC AGATCCCTGC ATCATGTGCT CATGTAGCCA GAGTACACTT
    AGCAGCACTA GTAGTGGAAA ATCTGTTATT GCTCCAAATA TGTACACTCT CTGTACTGTG
    ACACCCTCTC AGAGTGAAAC CAGTAGCATG AAATCTGAAT ATGCGGATCA GTGGAGTTCC
    TTTATTGACT GTTCACAGAC TCCAAAAGAG CAACCCAAAT CATTTGCAAA ACACAATCTA
    AGTAATCCTG ATGGTCTTAA TAATTACAGT GCATGTGAGC TGACCTATGG AATGAAGACC
    ATAGCACCAC ATGGGTACAC TGGATCAGTT AAAGTTAGTA ATTCCTCTTC AGAAAAAACA
    TACAGAGTTA CATCACCATC TAGTGGATAT TCAAGTCAGT CAAACACTCC CACCACATTG
    ACACCAGTGC CTCTTTTCTC AAAGAATATA TATCCAGAAA ATGGAAAATA CAAGTTAAAA
    CCAAAAGTAC CTGAAAGACG ATCTTCATTG GTATCCTCGG TTTCAGTTTC ATCTTCCTCT
    ACTTCCCTTT CTTCAAATAC ATTGGACTCT CTGCAGCAGA ACATGCAAAT TTCAACTGTT
    ATGTTGTCAG CCCATCACAC TCTTCCAACC CCACAACTCA CTCCTGACAT GCCCCCACCC
    CCAGCAATGA ATGCAACCAA CACAATTTTT GTCGAGTGTT CACCAAAATG TCCACCTCCT
    TCCCTTGAAT TTACAAGGCT AACAGCGGAT AGCATGGATA TGTGGCTTCC CTTCTCTCAA
    AACACTGCCA TGAGTAAACC TAAGGACAGT CTTCCATATC CTTCCTTTCC ACCTTCTCTT
    TCCATGATGC ACAGCATGGT CCCATCACCT GTACCTCCAC TTAACATTGA AATGCTGAAA
    ATTGAAACAG AATCATTTCC TCTCAGAAAT TCTGAAAGCA GTTATTTTGT ACATAATGAT
    CACAGTAAAC ATGAACTGTT GATACCAACT GTTCCTATAG TTACTTCTCA TGCACTAGAA
    ATGGTTCATT TGAGATCTGT GAAAAAAGCA TCACAACTGG AAACTAAAAC ATTAAGAGAA
    CACATGTCAG AATCCCACCT ACAGGATGAG ACATCCGCCT TATTGGTACA GCCATGTTTA
    GTACCAGCTG CTTCTCCAGA ACTAAATTCT AGTGAAAGCC AAATAGAAAA TTTCAGCATT
    CCTGCCCAGC AAGTCTCTGG TTTTAAAAGC AATGCAGTGA AACTAACTAT TGGTGAAATA
    GAAGCAGACA AGGCATATAA AGAGCACATA GAAAAAAATA CAAATTCAGC ATACAATGGA
    AACTCATCAG GGCCCAAATT ATCTCCTCAT GAGAAACCAA GTATGTCACA AAGCACACCA
    AATTCATCTC TTAGCAAGGA GCCGCCTCTT GTTTCAAAGA AGCCTAAACT TGTACTTATT
    GCCCCAACGC CACAACTTGA TTTGGCAGCA GAAAAAATAG CTCAAGTCAC TGAAAATGTG
    AGAAGCATGC CAAATGCCTT TGAAAAAGAT TCAGTTAGCA AATGTTGTGT GGGGGAGATA
    AATGACAGAA CTAAAGATGA AGACATAGCA GGTCCAACCA ATGGTTTGGC TCACCAAGAA
    CAAGAAGCCT GGTTATCCCC ATGCAAAGCT GCTAAAAATA TGATTTTCAT ACTACCATCA
    GTTCCAGAAA TTCAGAATCA TCAGCAAAAG GAAGAGCAGC AATCCATGTC AGATGAATTC
    AACATTAGTG GTAACATTTC TGAGACTGAG AATACAAGCT GCAACCAGAA TGCATCTCAA
    GATGATGAGA GTGCTGAAGT GTTTAATACA GAGACGGTGA ATTCAGTTCT CTGGACAAGC
    AGCGACATTA TGGGAAATGT AGAAGAGACC ACTCCAACAA GGCCTGCTAC AACTGAGGAT
    CTTTTTGCTC GTATCCATAG ATCCAAAAAA AAAATTCTTG GACGCAAAGA TTCTGACGAT
    GATCGTTGCA GAGATCACTC GCCTTCTTCT CCAGTTACCC CAACTGGTAA TATGCATCTA
    AGTTCCTTCA AGCAGACAGG TTCAATCCAA AGAAGTGTCA GAAAAACTAC AACAAGTAGT
    GACAGTTTCA AAGCACTGCT GTTAAAAAAG GGAAGCAGGT CTGAAAGTGG CTTCCGCATG
    TCTGCTGCAG AAATGCTGAA ACATACTGAT CCAAGGTTTC AGAGATCAAG ATCAGAATCA
    TCTCTGGAGT TTCCAGAAAG CCCCACATTG TCATCACCAA ACAAGAACAA GAAGGAGCAA
    CAGGAATGGG CAAAAAGTGA AGGGCTGATG CCTAGAAGTA TGTCTGGCTC TGTTGTCCGG
    TGCAGCCGCG CCAAGACTCC CCCTTCTGCA TCTAGTAGCA AATACAATGT CCGTAGTCGT
    CTCCAGAGTA GCCCAATGAC TGTTATATGT GAAGGAGAAG GAGAGGGTTC AGAAACAGTG
    GATAGCAGCT GTTCAAAGGG TCCTCTTTTG TTGAGCACAA AGAGTCTCGA TATCTGTTAC
    AGTGCTGATA AAGAAGTGAC CAACCACATA CTGCACATAA ACTTTAACAC ACTATTAGAA
    AAGAGCACAG ATGTTGCTAG TGGCTGCACA GGGGAAATAA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818259.1
    CDS94..4578
    Translation

    Target ORF information:

    RefSeq Version XM_012962805.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962805.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGTCG CAGTTCACAG TACTACTCCC AGGGACCAAC ATTCTCGACA CTCAGACTGG 
    ATTGCACAAG CTATCAGGAA GAGGAAAATA CACATGAGTC AAAGTGCAAA CCCACCATGG
    ACCCGCGGCC AGCCTTTTTC TGTTTCATTT AATGAAGAGG AACAGATCCT TTCAACCAAA
    AGGCCCAAGA CTCCTGTGTT GCTTGAGTTT TCTGAAACGC TCACTAACTG GACCAAGTCA
    CTGCCACTGC CTACTCCAGA GGAAAAGATG CGTCAGGAGG CTCAGGCAGT ACAGACAGAT
    GTAATTCCTA TAAACATAAC AGGGGAGAAT TTCAACCGCC AGGCCAGCTT TCAGCGGTCA
    CTTATTTATA CAGACACTGC CGTAAGATGT TCGAAGAAAG TCAAAAGGAG AAAGACTATA
    ACAGGCGTCC CAAACAACTT ACAGAAGGAG CTAGCAGGTA CTGGACACTC TGACCTAAGA
    AGGAATTCAG TGCTCATACC TGATGATTTT AATACTTTGG AACATTGTCA CCACTCAATG
    CAATTTCTGG ACACCAGAGA TTTTGGCTGC CAAACCAATA AAATAAAAAT AGTGCCACCA
    TCAATTAGAA GAATCAGAGC ACAGAAAGGG CAAGGAATTG GAATTCTGAT GTCCACTTCC
    TCTGGCAACA TGTCTACTCT AAGTGACTCT GCAGCTTGTT CTTCATATCT AAATGGTAAC
    CTGCACTTCC GTAGTCTGCC CAGGATTGGT GCAAGAGAAT CTCTTCAATC CCTTAACCAA
    AAAAATTATT ATTACAGCAA CATGGAGGAC CCTTTTACAA CTTTGCCTTG GCAGTTAAAC
    AAACTGCAAG TGGATGATAC TAATGTTCAA TTAAGGAGTA ACCCTAGGTC AACTATTAAT
    CAAAGGCCTG AGTCTCAAGA AGTAAAAAAT TCTACTAGTG AAATTGCCTC AAGTCCAGCA
    TGTATGGTCT CGCCTCATTC TTCTTATTCA ACAAACATTA TTCCAAATGC CATACTTTCA
    TATTCCTCAG AAGTAATAAG TATTCACACT CCCCACAGAG GTGGACTTCT GGACAAGAAA
    CCAAGAGTCC ACAGTTCATC TTCTAACAGA AATGGTATTA GCAGGGACTA TACTTCTGGC
    ATTAAAGATA ATTCCTCACC TGGTAACTGG CGTGAAAACA GTTCATCTAG GAACTCACAG
    ATATCAGATA CAAAGTCACC TATCAGTAAA AAAAGAGATG TTCCACACAA CGTTCCTTGC
    AAAATTAACA CTGGCTTTAA CAACTACTCA TTTGACAGTG ACAATCAAAG TGAATTAAGC
    TACTCAGATG GTCGTCAAAG AAATGGTAGT ATAACTAGCA GCATTAATAG TGCTGACAAC
    TGGTTGTATG AATCTAGGCA CAATGACGGT GCTCCATCTA GGAAACTATC TTCTGTAATT
    TCTGGGTCTC CTGTTAGCAA TATGAGCGCC TGCAGTTCTA AGGGGAAGGT AGATTCCAGC
    TCTTTGTATT TTCTGAACCA TGATGATTTT TGCACATCAA TGCATATAGA TTCTGGAATT
    AGTTTGACGG ACCAAAGCAT TCAGAATCTA CACTATAATA TAAATATCTT TGAAACAAAA
    AAAGCTGCAA ATATAGAAGT GAAAACAGGA TATAGTGACA AATCACTTCC ACGTAGCATA
    TCTTTAAAGA AATCTAAAAA TCCTCCCCCC CCACCTTCCA GGACAGACTC ACTTAGAAGG
    CCATCTAAGA AAGATATTCA GACCAGTGGG CAGGTGCTCA GTGAAACTCT TATTGCTACG
    CTTCAACAGT CCTTGCAACT AAACTTGAAG AGTAAGAGCA CTGGGTCCAC TTTGCAGTCA
    AGTCACTGTA TGGATTATGC AGATCCCTGC ATCATGTGCT CATGTAGCCA GAGTACACTT
    AGCAGCACTA GTAGTGGAAA ATCTGTTATT GCTCCAAATA TGTACACTCT CTGTACTGTG
    ACACCCTCTC AGAGTGAAAC CAGTAGCATG AAATCTGAAT ATGCGGATCA GTGGAGTTCC
    TTTATTGACT GTTCACAGAC TCCAAAAGAG CAACCCAAAT CATTTGCAAA ACACAATCTA
    AGTAATCCTG ATGGTCTTAA TAATTACAGT GCATGTGAGC TGACCTATGG AATGAAGACC
    ATAGCACCAC ATGGGTACAC TGGATCAGTT AAAGTTAGTA ATTCCTCTTC AGAAAAAACA
    TACAGAGTTA CATCACCATC TAGTGGATAT TCAAGTCAGT CAAACACTCC CACCACATTG
    ACACCAGTGC CTCTTTTCTC AAAGAATATA TATCCAGAAA ATGGAAAATA CAAGTTAAAA
    CCAAAAGTAC CTGAAAGACG ATCTTCATTG GTATCCTCGG TTTCAGTTTC ATCTTCCTCT
    ACTTCCCTTT CTTCAAATAC ATTGGACTCT CTGCAGCAGA ACATGCAAAT TTCAACTGTT
    ATGTTGTCAG CCCATCACAC TCTTCCAACC CCACAACTCA CTCCTGACAT GCCCCCACCC
    CCAGCAATGA ATGCAACCAA CACAATTTTT GTCGAGTGTT CACCAAAATG TCCACCTCCT
    TCCCTTGAAT TTACAAGGCT AACAGCGGAT AGCATGGATA TGTGGCTTCC CTTCTCTCAA
    AACACTGCCA TGAGTAAACC TAAGGACAGT CTTCCATATC CTTCCTTTCC ACCTTCTCTT
    TCCATGATGC ACAGCATGGT CCCATCACCT GTACCTCCAC TTAACATTGA AATGCTGAAA
    ATTGAAACAG AATCATTTCC TCTCAGAAAT TCTGAAAGCA GTTATTTTGT ACATAATGAT
    CACAGTAAAC ATGAACTGTT GATACCAACT GTTCCTATAG TTACTTCTCA TGCACTAGAA
    ATGGTTCATT TGAGATCTGT GAAAAAAGCA TCACAACTGG AAACTAAAAC ATTAAGAGAA
    CACATGTCAG AATCCCACCT ACAGGATGAG ACATCCGCCT TATTGGTACA GCCATGTTTA
    GTACCAGCTG CTTCTCCAGA ACTAAATTCT AGTGAAAGCC AAATAGAAAA TTTCAGCATT
    CCTGCCCAGC AAGTCTCTGG TTTTAAAAGC AATGCAGTGA AACTAACTAT TGGTGAAATA
    GAAGCAGACA AGGCATATAA AGAGCACATA GAAAAAAATA CAAATTCAGC ATACAATGGA
    AACTCATCAG GGCCCAAATT ATCTCCTCAT GAGAAACCAA GTATGTCACA AAGCACACCA
    AATTCATCTC TTAGCAAGGA GCCGCCTCTT GTTTCAAAGA AGCCTAAACT TGTACTTATT
    GCCCCAACGC CACAACTTGA TTTGGCAGCA GAAAAAATAG CTCAAGTCAC TGAAAATGTG
    AGAAGCATGC CAAATGCCTT TGAAAAAGAT TCAGTTAGCA AATGTTGTGT GGGGGAGATA
    AATGACAGAA CTAAAGATGA AGACATAGCA GGTCCAACCA ATGGTTTGGC TCACCAAGAA
    CAAGAAGCCT GGTTATCCCC ATGCAAAGCT GCTAAAAATA TGATTTTCAT ACTACCATCA
    GTTCCAGAAA TTCAGAATCA TCAGCAAAAG GAAGAGCAGC AATCCATGTC AGATGAATTC
    AACATTAGTG GTAACATTTC TGAGACTGAG AATACAAGCT GCAACCAGAA TGCATCTCAA
    GATGATGAGA GTGCTGAAGT GTTTAATACA GAGACGGTGA ATTCAGTTCT CTGGACAAGC
    AGCGACATTA TGGGAAATGT AGAAGAGACC ACTCCAACAA GGCCTGCTAC AACTGAGGAT
    CTTTTTGCTC GTATCCATAG ATCCAAAAAA AAAATTCTTG GACGCAAAGA TTCTGACGAT
    GATCGTTGCA GAGATCACTC GCCTTCTTCT CCAGTTACCC CAACTGGTAA TATGCATCTA
    AGTTCCTTCA AGCAGACAGG TTCAATCCAA AGAAGTGTCA GAAAAACTAC AACAAGTAGT
    GACAGTTTCA AAGCACTGCT GTTAAAAAAG GGAAGCAGGT CTGAAAGTGG CTTCCGCATG
    TCTGCTGCAG AAATGCTGAA ACATACTGAT CCAAGGTTTC AGAGATCAAG ATCAGAATCA
    TCTCTGGAGT TTCCAGAAAG CCCCACATTG TCATCACCAA ACAAGAACAA GAAGGAGCAA
    CAGGAATGGG CAAAAAGTGA AGGGCTGATG CCTAGAAGTA TGTCTGGCTC TGTTGTCCGG
    TGCAGCCGCG CCAAGACTCC CCCTTCTGCA TCTAGTAGCA AATACAATGT CCGTAGTCGT
    CTCCAGAGTA GCCCAATGAC TGTTATATGT GAAGGAGAAG GAGAGGGTTC AGAAACAGTG
    GATAGCAGCT GTTCAAAGGG TCCTCTTTTG TTGAGCACAA AGAGTCTCGA TATCTGTTAC
    AGTGCTGATA AAGAAGTGAC CAACCACATA CTGCACATAA ACTTTAACAC ACTATTAGAA
    AAGAGCACAG ATGTTGCTAG TGGCTGCACA GGGGAAATAA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32639
    Clone ID Related Accession (Same CDS sequence) XM_012962805.2 , XM_012962804.2
    Accession Version XM_012962804.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962804.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGTCG CAGTTCACAG TACTACTCCC AGGGACCAAC ATTCTCGACA CTCAGACTGG 
    ATTGCACAAG CTATCAGGAA GAGGAAAATA CACATGAGTC AAAGTGCAAA CCCACCATGG
    ACCCGCGGCC AGCCTTTTTC TGTTTCATTT AATGAAGAGG AACAGATCCT TTCAACCAAA
    AGGCCCAAGA CTCCTGTGTT GCTTGAGTTT TCTGAAACGC TCACTAACTG GACCAAGTCA
    CTGCCACTGC CTACTCCAGA GGAAAAGATG CGTCAGGAGG CTCAGGCAGT ACAGACAGAT
    GTAATTCCTA TAAACATAAC AGGGGAGAAT TTCAACCGCC AGGCCAGCTT TCAGCGGTCA
    CTTATTTATA CAGACACTGC CGTAAGATGT TCGAAGAAAG TCAAAAGGAG AAAGACTATA
    ACAGGCGTCC CAAACAACTT ACAGAAGGAG CTAGCAGGTA CTGGACACTC TGACCTAAGA
    AGGAATTCAG TGCTCATACC TGATGATTTT AATACTTTGG AACATTGTCA CCACTCAATG
    CAATTTCTGG ACACCAGAGA TTTTGGCTGC CAAACCAATA AAATAAAAAT AGTGCCACCA
    TCAATTAGAA GAATCAGAGC ACAGAAAGGG CAAGGAATTG GAATTCTGAT GTCCACTTCC
    TCTGGCAACA TGTCTACTCT AAGTGACTCT GCAGCTTGTT CTTCATATCT AAATGGTAAC
    CTGCACTTCC GTAGTCTGCC CAGGATTGGT GCAAGAGAAT CTCTTCAATC CCTTAACCAA
    AAAAATTATT ATTACAGCAA CATGGAGGAC CCTTTTACAA CTTTGCCTTG GCAGTTAAAC
    AAACTGCAAG TGGATGATAC TAATGTTCAA TTAAGGAGTA ACCCTAGGTC AACTATTAAT
    CAAAGGCCTG AGTCTCAAGA AGTAAAAAAT TCTACTAGTG AAATTGCCTC AAGTCCAGCA
    TGTATGGTCT CGCCTCATTC TTCTTATTCA ACAAACATTA TTCCAAATGC CATACTTTCA
    TATTCCTCAG AAGTAATAAG TATTCACACT CCCCACAGAG GTGGACTTCT GGACAAGAAA
    CCAAGAGTCC ACAGTTCATC TTCTAACAGA AATGGTATTA GCAGGGACTA TACTTCTGGC
    ATTAAAGATA ATTCCTCACC TGGTAACTGG CGTGAAAACA GTTCATCTAG GAACTCACAG
    ATATCAGATA CAAAGTCACC TATCAGTAAA AAAAGAGATG TTCCACACAA CGTTCCTTGC
    AAAATTAACA CTGGCTTTAA CAACTACTCA TTTGACAGTG ACAATCAAAG TGAATTAAGC
    TACTCAGATG GTCGTCAAAG AAATGGTAGT ATAACTAGCA GCATTAATAG TGCTGACAAC
    TGGTTGTATG AATCTAGGCA CAATGACGGT GCTCCATCTA GGAAACTATC TTCTGTAATT
    TCTGGGTCTC CTGTTAGCAA TATGAGCGCC TGCAGTTCTA AGGGGAAGGT AGATTCCAGC
    TCTTTGTATT TTCTGAACCA TGATGATTTT TGCACATCAA TGCATATAGA TTCTGGAATT
    AGTTTGACGG ACCAAAGCAT TCAGAATCTA CACTATAATA TAAATATCTT TGAAACAAAA
    AAAGCTGCAA ATATAGAAGT GAAAACAGGA TATAGTGACA AATCACTTCC ACGTAGCATA
    TCTTTAAAGA AATCTAAAAA TCCTCCCCCC CCACCTTCCA GGACAGACTC ACTTAGAAGG
    CCATCTAAGA AAGATATTCA GACCAGTGGG CAGGTGCTCA GTGAAACTCT TATTGCTACG
    CTTCAACAGT CCTTGCAACT AAACTTGAAG AGTAAGAGCA CTGGGTCCAC TTTGCAGTCA
    AGTCACTGTA TGGATTATGC AGATCCCTGC ATCATGTGCT CATGTAGCCA GAGTACACTT
    AGCAGCACTA GTAGTGGAAA ATCTGTTATT GCTCCAAATA TGTACACTCT CTGTACTGTG
    ACACCCTCTC AGAGTGAAAC CAGTAGCATG AAATCTGAAT ATGCGGATCA GTGGAGTTCC
    TTTATTGACT GTTCACAGAC TCCAAAAGAG CAACCCAAAT CATTTGCAAA ACACAATCTA
    AGTAATCCTG ATGGTCTTAA TAATTACAGT GCATGTGAGC TGACCTATGG AATGAAGACC
    ATAGCACCAC ATGGGTACAC TGGATCAGTT AAAGTTAGTA ATTCCTCTTC AGAAAAAACA
    TACAGAGTTA CATCACCATC TAGTGGATAT TCAAGTCAGT CAAACACTCC CACCACATTG
    ACACCAGTGC CTCTTTTCTC AAAGAATATA TATCCAGAAA ATGGAAAATA CAAGTTAAAA
    CCAAAAGTAC CTGAAAGACG ATCTTCATTG GTATCCTCGG TTTCAGTTTC ATCTTCCTCT
    ACTTCCCTTT CTTCAAATAC ATTGGACTCT CTGCAGCAGA ACATGCAAAT TTCAACTGTT
    ATGTTGTCAG CCCATCACAC TCTTCCAACC CCACAACTCA CTCCTGACAT GCCCCCACCC
    CCAGCAATGA ATGCAACCAA CACAATTTTT GTCGAGTGTT CACCAAAATG TCCACCTCCT
    TCCCTTGAAT TTACAAGGCT AACAGCGGAT AGCATGGATA TGTGGCTTCC CTTCTCTCAA
    AACACTGCCA TGAGTAAACC TAAGGACAGT CTTCCATATC CTTCCTTTCC ACCTTCTCTT
    TCCATGATGC ACAGCATGGT CCCATCACCT GTACCTCCAC TTAACATTGA AATGCTGAAA
    ATTGAAACAG AATCATTTCC TCTCAGAAAT TCTGAAAGCA GTTATTTTGT ACATAATGAT
    CACAGTAAAC ATGAACTGTT GATACCAACT GTTCCTATAG TTACTTCTCA TGCACTAGAA
    ATGGTTCATT TGAGATCTGT GAAAAAAGCA TCACAACTGG AAACTAAAAC ATTAAGAGAA
    CACATGTCAG AATCCCACCT ACAGGATGAG ACATCCGCCT TATTGGTACA GCCATGTTTA
    GTACCAGCTG CTTCTCCAGA ACTAAATTCT AGTGAAAGCC AAATAGAAAA TTTCAGCATT
    CCTGCCCAGC AAGTCTCTGG TTTTAAAAGC AATGCAGTGA AACTAACTAT TGGTGAAATA
    GAAGCAGACA AGGCATATAA AGAGCACATA GAAAAAAATA CAAATTCAGC ATACAATGGA
    AACTCATCAG GGCCCAAATT ATCTCCTCAT GAGAAACCAA GTATGTCACA AAGCACACCA
    AATTCATCTC TTAGCAAGGA GCCGCCTCTT GTTTCAAAGA AGCCTAAACT TGTACTTATT
    GCCCCAACGC CACAACTTGA TTTGGCAGCA GAAAAAATAG CTCAAGTCAC TGAAAATGTG
    AGAAGCATGC CAAATGCCTT TGAAAAAGAT TCAGTTAGCA AATGTTGTGT GGGGGAGATA
    AATGACAGAA CTAAAGATGA AGACATAGCA GGTCCAACCA ATGGTTTGGC TCACCAAGAA
    CAAGAAGCCT GGTTATCCCC ATGCAAAGCT GCTAAAAATA TGATTTTCAT ACTACCATCA
    GTTCCAGAAA TTCAGAATCA TCAGCAAAAG GAAGAGCAGC AATCCATGTC AGATGAATTC
    AACATTAGTG GTAACATTTC TGAGACTGAG AATACAAGCT GCAACCAGAA TGCATCTCAA
    GATGATGAGA GTGCTGAAGT GTTTAATACA GAGACGGTGA ATTCAGTTCT CTGGACAAGC
    AGCGACATTA TGGGAAATGT AGAAGAGACC ACTCCAACAA GGCCTGCTAC AACTGAGGAT
    CTTTTTGCTC GTATCCATAG ATCCAAAAAA AAAATTCTTG GACGCAAAGA TTCTGACGAT
    GATCGTTGCA GAGATCACTC GCCTTCTTCT CCAGTTACCC CAACTGGTAA TATGCATCTA
    AGTTCCTTCA AGCAGACAGG TTCAATCCAA AGAAGTGTCA GAAAAACTAC AACAAGTAGT
    GACAGTTTCA AAGCACTGCT GTTAAAAAAG GGAAGCAGGT CTGAAAGTGG CTTCCGCATG
    TCTGCTGCAG AAATGCTGAA ACATACTGAT CCAAGGTTTC AGAGATCAAG ATCAGAATCA
    TCTCTGGAGT TTCCAGAAAG CCCCACATTG TCATCACCAA ACAAGAACAA GAAGGAGCAA
    CAGGAATGGG CAAAAAGTGA AGGGCTGATG CCTAGAAGTA TGTCTGGCTC TGTTGTCCGG
    TGCAGCCGCG CCAAGACTCC CCCTTCTGCA TCTAGTAGCA AATACAATGT CCGTAGTCGT
    CTCCAGAGTA GCCCAATGAC TGTTATATGT GAAGGAGAAG GAGAGGGTTC AGAAACAGTG
    GATAGCAGCT GTTCAAAGGG TCCTCTTTTG TTGAGCACAA AGAGTCTCGA TATCTGTTAC
    AGTGCTGATA AAGAAGTGAC CAACCACATA CTGCACATAA ACTTTAACAC ACTATTAGAA
    AAGAGCACAG ATGTTGCTAG TGGCTGCACA GGGGAAATAA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818258.1
    CDS749..5233
    Translation

    Target ORF information:

    RefSeq Version XM_012962804.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962804.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGTCG CAGTTCACAG TACTACTCCC AGGGACCAAC ATTCTCGACA CTCAGACTGG 
    ATTGCACAAG CTATCAGGAA GAGGAAAATA CACATGAGTC AAAGTGCAAA CCCACCATGG
    ACCCGCGGCC AGCCTTTTTC TGTTTCATTT AATGAAGAGG AACAGATCCT TTCAACCAAA
    AGGCCCAAGA CTCCTGTGTT GCTTGAGTTT TCTGAAACGC TCACTAACTG GACCAAGTCA
    CTGCCACTGC CTACTCCAGA GGAAAAGATG CGTCAGGAGG CTCAGGCAGT ACAGACAGAT
    GTAATTCCTA TAAACATAAC AGGGGAGAAT TTCAACCGCC AGGCCAGCTT TCAGCGGTCA
    CTTATTTATA CAGACACTGC CGTAAGATGT TCGAAGAAAG TCAAAAGGAG AAAGACTATA
    ACAGGCGTCC CAAACAACTT ACAGAAGGAG CTAGCAGGTA CTGGACACTC TGACCTAAGA
    AGGAATTCAG TGCTCATACC TGATGATTTT AATACTTTGG AACATTGTCA CCACTCAATG
    CAATTTCTGG ACACCAGAGA TTTTGGCTGC CAAACCAATA AAATAAAAAT AGTGCCACCA
    TCAATTAGAA GAATCAGAGC ACAGAAAGGG CAAGGAATTG GAATTCTGAT GTCCACTTCC
    TCTGGCAACA TGTCTACTCT AAGTGACTCT GCAGCTTGTT CTTCATATCT AAATGGTAAC
    CTGCACTTCC GTAGTCTGCC CAGGATTGGT GCAAGAGAAT CTCTTCAATC CCTTAACCAA
    AAAAATTATT ATTACAGCAA CATGGAGGAC CCTTTTACAA CTTTGCCTTG GCAGTTAAAC
    AAACTGCAAG TGGATGATAC TAATGTTCAA TTAAGGAGTA ACCCTAGGTC AACTATTAAT
    CAAAGGCCTG AGTCTCAAGA AGTAAAAAAT TCTACTAGTG AAATTGCCTC AAGTCCAGCA
    TGTATGGTCT CGCCTCATTC TTCTTATTCA ACAAACATTA TTCCAAATGC CATACTTTCA
    TATTCCTCAG AAGTAATAAG TATTCACACT CCCCACAGAG GTGGACTTCT GGACAAGAAA
    CCAAGAGTCC ACAGTTCATC TTCTAACAGA AATGGTATTA GCAGGGACTA TACTTCTGGC
    ATTAAAGATA ATTCCTCACC TGGTAACTGG CGTGAAAACA GTTCATCTAG GAACTCACAG
    ATATCAGATA CAAAGTCACC TATCAGTAAA AAAAGAGATG TTCCACACAA CGTTCCTTGC
    AAAATTAACA CTGGCTTTAA CAACTACTCA TTTGACAGTG ACAATCAAAG TGAATTAAGC
    TACTCAGATG GTCGTCAAAG AAATGGTAGT ATAACTAGCA GCATTAATAG TGCTGACAAC
    TGGTTGTATG AATCTAGGCA CAATGACGGT GCTCCATCTA GGAAACTATC TTCTGTAATT
    TCTGGGTCTC CTGTTAGCAA TATGAGCGCC TGCAGTTCTA AGGGGAAGGT AGATTCCAGC
    TCTTTGTATT TTCTGAACCA TGATGATTTT TGCACATCAA TGCATATAGA TTCTGGAATT
    AGTTTGACGG ACCAAAGCAT TCAGAATCTA CACTATAATA TAAATATCTT TGAAACAAAA
    AAAGCTGCAA ATATAGAAGT GAAAACAGGA TATAGTGACA AATCACTTCC ACGTAGCATA
    TCTTTAAAGA AATCTAAAAA TCCTCCCCCC CCACCTTCCA GGACAGACTC ACTTAGAAGG
    CCATCTAAGA AAGATATTCA GACCAGTGGG CAGGTGCTCA GTGAAACTCT TATTGCTACG
    CTTCAACAGT CCTTGCAACT AAACTTGAAG AGTAAGAGCA CTGGGTCCAC TTTGCAGTCA
    AGTCACTGTA TGGATTATGC AGATCCCTGC ATCATGTGCT CATGTAGCCA GAGTACACTT
    AGCAGCACTA GTAGTGGAAA ATCTGTTATT GCTCCAAATA TGTACACTCT CTGTACTGTG
    ACACCCTCTC AGAGTGAAAC CAGTAGCATG AAATCTGAAT ATGCGGATCA GTGGAGTTCC
    TTTATTGACT GTTCACAGAC TCCAAAAGAG CAACCCAAAT CATTTGCAAA ACACAATCTA
    AGTAATCCTG ATGGTCTTAA TAATTACAGT GCATGTGAGC TGACCTATGG AATGAAGACC
    ATAGCACCAC ATGGGTACAC TGGATCAGTT AAAGTTAGTA ATTCCTCTTC AGAAAAAACA
    TACAGAGTTA CATCACCATC TAGTGGATAT TCAAGTCAGT CAAACACTCC CACCACATTG
    ACACCAGTGC CTCTTTTCTC AAAGAATATA TATCCAGAAA ATGGAAAATA CAAGTTAAAA
    CCAAAAGTAC CTGAAAGACG ATCTTCATTG GTATCCTCGG TTTCAGTTTC ATCTTCCTCT
    ACTTCCCTTT CTTCAAATAC ATTGGACTCT CTGCAGCAGA ACATGCAAAT TTCAACTGTT
    ATGTTGTCAG CCCATCACAC TCTTCCAACC CCACAACTCA CTCCTGACAT GCCCCCACCC
    CCAGCAATGA ATGCAACCAA CACAATTTTT GTCGAGTGTT CACCAAAATG TCCACCTCCT
    TCCCTTGAAT TTACAAGGCT AACAGCGGAT AGCATGGATA TGTGGCTTCC CTTCTCTCAA
    AACACTGCCA TGAGTAAACC TAAGGACAGT CTTCCATATC CTTCCTTTCC ACCTTCTCTT
    TCCATGATGC ACAGCATGGT CCCATCACCT GTACCTCCAC TTAACATTGA AATGCTGAAA
    ATTGAAACAG AATCATTTCC TCTCAGAAAT TCTGAAAGCA GTTATTTTGT ACATAATGAT
    CACAGTAAAC ATGAACTGTT GATACCAACT GTTCCTATAG TTACTTCTCA TGCACTAGAA
    ATGGTTCATT TGAGATCTGT GAAAAAAGCA TCACAACTGG AAACTAAAAC ATTAAGAGAA
    CACATGTCAG AATCCCACCT ACAGGATGAG ACATCCGCCT TATTGGTACA GCCATGTTTA
    GTACCAGCTG CTTCTCCAGA ACTAAATTCT AGTGAAAGCC AAATAGAAAA TTTCAGCATT
    CCTGCCCAGC AAGTCTCTGG TTTTAAAAGC AATGCAGTGA AACTAACTAT TGGTGAAATA
    GAAGCAGACA AGGCATATAA AGAGCACATA GAAAAAAATA CAAATTCAGC ATACAATGGA
    AACTCATCAG GGCCCAAATT ATCTCCTCAT GAGAAACCAA GTATGTCACA AAGCACACCA
    AATTCATCTC TTAGCAAGGA GCCGCCTCTT GTTTCAAAGA AGCCTAAACT TGTACTTATT
    GCCCCAACGC CACAACTTGA TTTGGCAGCA GAAAAAATAG CTCAAGTCAC TGAAAATGTG
    AGAAGCATGC CAAATGCCTT TGAAAAAGAT TCAGTTAGCA AATGTTGTGT GGGGGAGATA
    AATGACAGAA CTAAAGATGA AGACATAGCA GGTCCAACCA ATGGTTTGGC TCACCAAGAA
    CAAGAAGCCT GGTTATCCCC ATGCAAAGCT GCTAAAAATA TGATTTTCAT ACTACCATCA
    GTTCCAGAAA TTCAGAATCA TCAGCAAAAG GAAGAGCAGC AATCCATGTC AGATGAATTC
    AACATTAGTG GTAACATTTC TGAGACTGAG AATACAAGCT GCAACCAGAA TGCATCTCAA
    GATGATGAGA GTGCTGAAGT GTTTAATACA GAGACGGTGA ATTCAGTTCT CTGGACAAGC
    AGCGACATTA TGGGAAATGT AGAAGAGACC ACTCCAACAA GGCCTGCTAC AACTGAGGAT
    CTTTTTGCTC GTATCCATAG ATCCAAAAAA AAAATTCTTG GACGCAAAGA TTCTGACGAT
    GATCGTTGCA GAGATCACTC GCCTTCTTCT CCAGTTACCC CAACTGGTAA TATGCATCTA
    AGTTCCTTCA AGCAGACAGG TTCAATCCAA AGAAGTGTCA GAAAAACTAC AACAAGTAGT
    GACAGTTTCA AAGCACTGCT GTTAAAAAAG GGAAGCAGGT CTGAAAGTGG CTTCCGCATG
    TCTGCTGCAG AAATGCTGAA ACATACTGAT CCAAGGTTTC AGAGATCAAG ATCAGAATCA
    TCTCTGGAGT TTCCAGAAAG CCCCACATTG TCATCACCAA ACAAGAACAA GAAGGAGCAA
    CAGGAATGGG CAAAAAGTGA AGGGCTGATG CCTAGAAGTA TGTCTGGCTC TGTTGTCCGG
    TGCAGCCGCG CCAAGACTCC CCCTTCTGCA TCTAGTAGCA AATACAATGT CCGTAGTCGT
    CTCCAGAGTA GCCCAATGAC TGTTATATGT GAAGGAGAAG GAGAGGGTTC AGAAACAGTG
    GATAGCAGCT GTTCAAAGGG TCCTCTTTTG TTGAGCACAA AGAGTCTCGA TATCTGTTAC
    AGTGCTGATA AAGAAGTGAC CAACCACATA CTGCACATAA ACTTTAACAC ACTATTAGAA
    AAGAGCACAG ATGTTGCTAG TGGCTGCACA GGGGAAATAA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32641
    Clone ID Related Accession (Same CDS sequence) XM_012962807.2 , XM_012962807.3
    Accession Version XM_012962807.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962807.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GAGAGATGCC CAAAGAGATG
    CTCAGAAGCG GATCTGCCAG GGGGGCGGGG GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818261.1
    CDS639..4934
    Translation

    Target ORF information:

    RefSeq Version XM_012962807.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962807.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GAGAGATGCC CAAAGAGATG
    CTCAGAAGCG GATCTGCCAG GGGGGCGGGG GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32642
    Clone ID Related Accession (Same CDS sequence) XM_012962809.3 , XM_012962809.2
    Accession Version XM_012962809.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NHS-like protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GTGTGTGCAC ATGTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818263.1
    CDS638..4897
    Translation

    Target ORF information:

    RefSeq Version XM_012962809.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962809.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
    GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA
    TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT
    TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC
    AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG
    GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA
    CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA
    GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT
    CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA
    AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG
    GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG
    CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG
    GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC
    CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA
    GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT
    ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG
    GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT
    AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT
    GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT
    TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA
    TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA
    ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT
    AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT
    GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT
    ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA
    GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT
    AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC
    AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT
    GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT
    GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC
    AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT
    AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT
    AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA
    ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT
    ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC
    AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC
    AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC
    AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC
    ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC
    TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT
    ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA
    TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA
    TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG
    CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT
    AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG
    TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA
    AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG
    GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG
    AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC
    ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT
    TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT
    ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT
    CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA
    CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC
    AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA
    GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG
    GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC
    TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC
    CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG
    AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT
    ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA
    AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG
    AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA
    GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC
    AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC
    AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT
    ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG
    CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC
    TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG
    AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA
    AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GTGTGTGCAC ATGTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.