nhsl1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012962802.2	XP_012818256.1	NHS-like protein 1 isoform X3
XM_012962807.2	XP_012818261.1	NHS-like protein 1 isoform X6
XM_012962805.2	XP_012818259.1	NHS-like protein 1 isoform X4
XM_012962807.3	XP_012818261.1	NHS-like protein 1 isoform X5
XM_012962809.3	XP_012818263.1	NHS-like protein 1 isoform X6
XM_012962803.2	XP_012818257.1	NHS-like protein 1 isoform X4
XM_012962800.2	XP_012818254.1	NHS-like protein 1 isoform X1
XM_031901570.1	XP_031757430.1	NHS-like protein 1 isoform X7
XM_012962809.2	XP_012818263.1	NHS-like protein 1 isoform X7
NM_001142125.1	NP_001135597.1	NHS-like protein 1
XM_012962801.2	XP_012818255.1	NHS-like protein 1 isoform X2
XM_012962802.3	XP_012818256.1	NHS-like protein 1 isoform X3
XM_012962804.2	XP_012818258.1	NHS-like protein 1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa32641	XM_012962807.3 Latest version!	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32642	XM_012962809.3 Latest version!	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa57932	XM_031901570.1 Latest version!	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32637	XM_012962802.3 Latest version!	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa21753	NM_001142125.1 Latest version!	Xenopus tropicalis NHS like 1 (nhsl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa32635	XM_012962800.2 Latest version!	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa32636	XM_012962801.2 Latest version!	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa32638	XM_012962803.2 Latest version!	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa32639	XM_012962805.2 Latest version!	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32639	XM_012962804.2 Latest version!	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OXa32641	XM_012962807.2	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32642	XM_012962809.2	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32637	XM_012962802.2	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

nhsl1 ( XM_012962807.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818261.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa32641
Clone ID Related Accession (Same CDS sequence)	XM_012962807.2 , XM_012962807.3
Accession Version	XM_012962807.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962807.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GAGAGATGCC CAAAGAGATG 
CTCAGAAGCG GATCTGCCAG GGGGGCGGGG GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818261.1
CDS	636..4931
Translation	MPFHLRLVQPIYVCRLGDPDAQAPRGGNYLIFNSLEDVSSYSLRQIVRQLSDLCRHAENIFSSIHFEASSLGDRTTRLKHRISFLQHNISHLDHKRVTVPVSNLDEESKWTVHYTASCHQQENVFLSSSRPLCVDDLHRQAKVNLKTVLRECDKLRKDGCRSSQYYSQGPTFSTLRLDCTSYQEEENTHESKFSVSFNEEEQILSTKRPKTPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHREMPKEMLRSGSARGAGG

Target ORF information:

RefSeq Version	XM_012962807.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962807.3

ORF Insert Sequence:

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GAGAGATGCC CAAAGAGATG 
CTCAGAAGCG GATCTGCCAG GGGGGCGGGG GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_012962809.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818263.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa32642
Clone ID Related Accession (Same CDS sequence)	XM_012962809.3 , XM_012962809.2
Accession Version	XM_012962809.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GTGTGTGCAC ATGTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818263.1
CDS	636..4895
Translation	MPFHLRLVQPIYVCRLGDPDAQAPRGGNYLIFNSLEDVSSYSLRQIVRQLSDLCRHAENIFSSIHFEASSLGDRTTRLKHRISFLQHNISHLDHKRVTVPVSNLDEESKWTVHYTASCHQQENVFLSSSRPLCVDDLHRQAKVNLKTVLRECDKLRKDGCRSSQYYSQGPTFSTLRLDCTSYQEEENTHESKFSVSFNEEEQILSTKRPKTPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHSVCTCV

Target ORF information:

RefSeq Version	XM_012962809.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962809.3

ORF Insert Sequence:

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GTGTGTGCAC ATGTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_031901570.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757430.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X8, mRNA.

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CloneID	OXa57932
Clone ID Related Accession (Same CDS sequence)	XM_031901570.1
Accession Version	XM_031901570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTCAGG AGGCTCAGGC AGTACAGACA GATGTAATTC CTATAAACAT AACAGGGGAG 
AATTTCAACC GCCAGGCCAG CTTTCAGCGG TCACTTATTT ATACAGACAC TGCCGTAAGA 
TGTTCGAAGA AAGTCAAAAG GAGAAAGACT ATAACAGGCG TCCCAAACAA CTTACAGAAG 
GAGCTAGCAG GTACTGGACA CTCTGACCTA AGAAGGAATT CAGTGCTCAT ACCTGATGAT 
TTTAATACTT TGGAACATTG TCACCACTCA ATGCAATTTC TGGACACCAG AGATTTTGGC 
TGCCAAACCA ATAAAATAAA AATAGTGCCA CCATCAATTA GAAGAATCAG AGCACAGAAA 
GGGCAAGGAA TTGGAATTCT GATGTCCACT TCCTCTGGCA ACATGTCTAC TCTAAGTGAC 
TCTGCAGCTT GTTCTTCATA TCTAAATGGT AACCTGCACT TCCGTAGTCT GCCCAGGATT 
GGTGCAAGAG AATCTCTTCA ATCCCTTAAC CAAAAAAATT ATTATTACAG CAACATGGAG 
GACCCTTTTA CAACTTTGCC TTGGCAGTTA AACAAACTGC AAGTGGATGA TACTAATGTT 
CAATTAAGGA GTAACCCTAG GTCAACTATT AATCAAAGGC CTGAGTCTCA AGAAGTAAAA 
AATTCTACTA GTGAAATTGC CTCAAGTCCA GCATGTATGG TCTCGCCTCA TTCTTCTTAT 
TCAACAAACA TTATTCCAAA TGCCATACTT TCATATTCCT CAGAAGTAAT AAGTATTCAC 
ACTCCCCACA GAGGTGGACT TCTGGACAAG AAACCAAGAG TCCACAGTTC ATCTTCTAAC 
AGAAATGGTA TTAGCAGGGA CTATACTTCT GGCATTAAAG ATAATTCCTC ACCTGGTAAC 
TGGCGTGAAA ACAGTTCATC TAGGAACTCA CAGATATCAG ATACAAAGTC ACCTATCAGT 
AAAAAAAGAG ATGTTCCACA CAACGTTCCT TGCAAAATTA ACACTGGCTT TAACAACTAC 
TCATTTGACA GTGACAATCA AAGTGAATTA AGCTACTCAG ATGGTCGTCA AAGAAATGGT 
AGTATAACTA GCAGCATTAA TAGTGCTGAC AACTGGTTGT ATGAATCTAG GCACAATGAC 
GGTGCTCCAT CTAGGAAACT ATCTTCTGTA ATTTCTGGGT CTCCTGTTAG CAATATGAGC 
GCCTGCAGTT CTAAGGGGAA GGTAGATTCC AGCTCTTTGT ATTTTCTGAA CCATGATGAT 
TTTTGCACAT CAATGCATAT AGATTCTGGA ATTAGTTTGA CGGACCAAAG CATTCAGAAT 
CTACACTATA ATATAAATAT CTTTGAAACA AAAAAAGCTG CAAATATAGA AGTGAAAACA 
GGATATAGTG ACAAATCACT TCCACGTAGC ATATCTTTAA AGAAATCTAA AAATCCTCCC 
CCCCCACCTT CCAGGACAGA CTCACTTAGA AGGCCATCTA AGAAAGATAT TCAGACCAGT 
GGGCAGGTGC TCAGTGAAAC TCTTATTGCT ACGCTTCAAC AGTCCTTGCA ACTAAACTTG 
AAGAGTAAGA GCACTGGGTC CACTTTGCAG TCAAGTCACT GTATGGATTA TGCAGATCCC 
TGCATCATGT GCTCATGTAG CCAGAGTACA CTTAGCAGCA CTAGTAGTGG AAAATCTGTT 
ATTGCTCCAA ATATGTACAC TCTCTGTACT GTGACACCCT CTCAGAGTGA AACCAGTAGC 
ATGAAATCTG AATATGCGGA TCAGTGGAGT TCCTTTATTG ACTGTTCACA GACTCCAAAA 
GAGCAACCCA AATCATTTGC AAAACACAAT CTAAGTAATC CTGATGGTCT TAATAATTAC 
AGTGCATGTG AGCTGACCTA TGGAATGAAG ACCATAGCAC CACATGGGTA CACTGGATCA 
GTTAAAGTTA GTAATTCCTC TTCAGAAAAA ACATACAGAG TTACATCACC ATCTAGTGGA 
TATTCAAGTC AGTCAAACAC TCCCACCACA TTGACACCAG TGCCTCTTTT CTCAAAGAAT 
ATATATCCAG AAAATGGAAA ATACAAGTTA AAACCAAAAG TACCTGAAAG ACGATCTTCA 
TTGGTATCCT CGGTTTCAGT TTCATCTTCC TCTACTTCCC TTTCTTCAAA TACATTGGAC 
TCTCTGCAGC AGAACATGCA AATTTCAACT GTTATGTTGT CAGCCCATCA CACTCTTCCA 
ACCCCACAAC TCACTCCTGA CATGCCCCCA CCCCCAGCAA TGAATGCAAC CAACACAATT 
TTTGTCGAGT GTTCACCAAA ATGTCCACCT CCTTCCCTTG AATTTACAAG GCTAACAGCG 
GATAGCATGG ATATGTGGCT TCCCTTCTCT CAAAACACTG CCATGAGTAA ACCTAAGGAC 
AGTCTTCCAT ATCCTTCCTT TCCACCTTCT CTTTCCATGA TGCACAGCAT GGTCCCATCA 
CCTGTACCTC CACTTAACAT TGAAATGCTG AAAATTGAAA CAGAATCATT TCCTCTCAGA 
AATTCTGAAA GCAGTTATTT TGTACATAAT GATCACAGTA AACATGAACT GTTGATACCA 
ACTGTTCCTA TAGTTACTTC TCATGCACTA GAAATGGTTC ATTTGAGATC TGTGAAAAAA 
GCATCACAAC TGGAAACTAA AACATTAAGA GAACACATGT CAGAATCCCA CCTACAGGAT 
GAGACATCCG CCTTATTGGT ACAGCCATGT TTAGTACCAG CTGCTTCTCC AGAACTAAAT 
TCTAGTGAAA GCCAAATAGA AAATTTCAGC ATTCCTGCCC AGCAAGTCTC TGGTTTTAAA 
AGCAATGCAG TGAAACTAAC TATTGGTGAA ATAGAAGCAG ACAAGGCATA TAAAGAGCAC 
ATAGAAAAAA ATACAAATTC AGCATACAAT GGAAACTCAT CAGGGCCCAA ATTATCTCCT 
CATGAGAAAC CAAGTATGTC ACAAAGCACA CCAAATTCAT CTCTTAGCAA GGAGCCGCCT 
CTTGTTTCAA AGAAGCCTAA ACTTGTACTT ATTGCCCCAA CGCCACAACT TGATTTGGCA 
GCAGAAAAAA TAGCTCAAGT CACTGAAAAT GTGAGAAGCA TGCCAAATGC CTTTGAAAAA 
GATTCAGTTA GCAAATGTTG TGTGGGGGAG ATAAATGACA GAACTAAAGA TGAAGACATA 
GCAGGTCCAA CCAATGGTTT GGCTCACCAA GAACAAGAAG CCTGGTTATC CCCATGCAAA 
GCTGCTAAAA ATATGATTTT CATACTACCA TCAGTTCCAG AAATTCAGAA TCATCAGCAA 
AAGGAAGAGC AGCAATCCAT GTCAGATGAA TTCAACATTA GTGGTAACAT TTCTGAGACT 
GAGAATACAA GCTGCAACCA GAATGCATCT CAAGATGATG AGAGTGCTGA AGTGTTTAAT 
ACAGAGACGG TGAATTCAGT TCTCTGGACA AGCAGCGACA TTATGGGAAA TGTAGAAGAG 
ACCACTCCAA CAAGGCCTGC TACAACTGAG GATCTTTTTG CTCGTATCCA TAGATCCAAA 
AAAAAAATTC TTGGACGCAA AGATTCTGAC GATGATCGTT GCAGAGATCA CTCGCCTTCT 
TCTCCAGTTA CCCCAACTGG TAATATGCAT CTAAGTTCCT TCAAGCAGAC AGGTTCAATC 
CAAAGAAGTG TCAGAAAAAC TACAACAAGT AGTGACAGTT TCAAAGCACT GCTGTTAAAA 
AAGGGAAGCA GGTCTGAAAG TGGCTTCCGC ATGTCTGCTG CAGAAATGCT GAAACATACT 
GATCCAAGGT TTCAGAGATC AAGATCAGAA TCATCTCTGG AGTTTCCAGA AAGCCCCACA 
TTGTCATCAC CAAACAAGAA CAAGAAGGAG CAACAGGAAT GGGCAAAAAG TGAAGGGCTG 
ATGCCTAGAA GTATGTCTGG CTCTGTTGTC CGGTGCAGCC GCGCCAAGAC TCCCCCTTCT 
GCATCTAGTA GCAAATACAA TGTCCGTAGT CGTCTCCAGA GTAGCCCAAT GACTGTTATA 
TGTGAAGGAG AAGGAGAGGG TTCAGAAACA GTGGATAGCA GCTGTTCAAA GGGTCCTCTT 
TTGTTGAGCA CAAAGAGTCT CGATATCTGT TACAGTGCTG ATAAAGAAGT GACCAACCAC 
ATACTGCACA TAAACTTTAA CACACTATTA GAAAAGAGCA CAGATGTTGC TAGTGGCTGC 
ACAGGGGAAA TAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757430.1
CDS	309..4526
Translation	MRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHRSKKKILGRKDSDDDRCRDHSPSSPVTPTGNMHLSSFKQTGSIQRSVRKTTTSSDSFKALLLKKGSRSESGFRMSAAEMLKHTDPRFQRSRSESSLEFPESPTLSSPNKNKKEQQEWAKSEGLMPRSMSGSVVRCSRAKTPPSASSSKYNVRSRLQSSPMTVICEGEGEGSETVDSSCSKGPLLLSTKSLDICYSADKEVTNHILHINFNTLLEKSTDVASGCTGEII

Target ORF information:

RefSeq Version	XM_031901570.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901570.1

ORF Insert Sequence:

ATGCGTCAGG AGGCTCAGGC AGTACAGACA GATGTAATTC CTATAAACAT AACAGGGGAG 
AATTTCAACC GCCAGGCCAG CTTTCAGCGG TCACTTATTT ATACAGACAC TGCCGTAAGA 
TGTTCGAAGA AAGTCAAAAG GAGAAAGACT ATAACAGGCG TCCCAAACAA CTTACAGAAG 
GAGCTAGCAG GTACTGGACA CTCTGACCTA AGAAGGAATT CAGTGCTCAT ACCTGATGAT 
TTTAATACTT TGGAACATTG TCACCACTCA ATGCAATTTC TGGACACCAG AGATTTTGGC 
TGCCAAACCA ATAAAATAAA AATAGTGCCA CCATCAATTA GAAGAATCAG AGCACAGAAA 
GGGCAAGGAA TTGGAATTCT GATGTCCACT TCCTCTGGCA ACATGTCTAC TCTAAGTGAC 
TCTGCAGCTT GTTCTTCATA TCTAAATGGT AACCTGCACT TCCGTAGTCT GCCCAGGATT 
GGTGCAAGAG AATCTCTTCA ATCCCTTAAC CAAAAAAATT ATTATTACAG CAACATGGAG 
GACCCTTTTA CAACTTTGCC TTGGCAGTTA AACAAACTGC AAGTGGATGA TACTAATGTT 
CAATTAAGGA GTAACCCTAG GTCAACTATT AATCAAAGGC CTGAGTCTCA AGAAGTAAAA 
AATTCTACTA GTGAAATTGC CTCAAGTCCA GCATGTATGG TCTCGCCTCA TTCTTCTTAT 
TCAACAAACA TTATTCCAAA TGCCATACTT TCATATTCCT CAGAAGTAAT AAGTATTCAC 
ACTCCCCACA GAGGTGGACT TCTGGACAAG AAACCAAGAG TCCACAGTTC ATCTTCTAAC 
AGAAATGGTA TTAGCAGGGA CTATACTTCT GGCATTAAAG ATAATTCCTC ACCTGGTAAC 
TGGCGTGAAA ACAGTTCATC TAGGAACTCA CAGATATCAG ATACAAAGTC ACCTATCAGT 
AAAAAAAGAG ATGTTCCACA CAACGTTCCT TGCAAAATTA ACACTGGCTT TAACAACTAC 
TCATTTGACA GTGACAATCA AAGTGAATTA AGCTACTCAG ATGGTCGTCA AAGAAATGGT 
AGTATAACTA GCAGCATTAA TAGTGCTGAC AACTGGTTGT ATGAATCTAG GCACAATGAC 
GGTGCTCCAT CTAGGAAACT ATCTTCTGTA ATTTCTGGGT CTCCTGTTAG CAATATGAGC 
GCCTGCAGTT CTAAGGGGAA GGTAGATTCC AGCTCTTTGT ATTTTCTGAA CCATGATGAT 
TTTTGCACAT CAATGCATAT AGATTCTGGA ATTAGTTTGA CGGACCAAAG CATTCAGAAT 
CTACACTATA ATATAAATAT CTTTGAAACA AAAAAAGCTG CAAATATAGA AGTGAAAACA 
GGATATAGTG ACAAATCACT TCCACGTAGC ATATCTTTAA AGAAATCTAA AAATCCTCCC 
CCCCCACCTT CCAGGACAGA CTCACTTAGA AGGCCATCTA AGAAAGATAT TCAGACCAGT 
GGGCAGGTGC TCAGTGAAAC TCTTATTGCT ACGCTTCAAC AGTCCTTGCA ACTAAACTTG 
AAGAGTAAGA GCACTGGGTC CACTTTGCAG TCAAGTCACT GTATGGATTA TGCAGATCCC 
TGCATCATGT GCTCATGTAG CCAGAGTACA CTTAGCAGCA CTAGTAGTGG AAAATCTGTT 
ATTGCTCCAA ATATGTACAC TCTCTGTACT GTGACACCCT CTCAGAGTGA AACCAGTAGC 
ATGAAATCTG AATATGCGGA TCAGTGGAGT TCCTTTATTG ACTGTTCACA GACTCCAAAA 
GAGCAACCCA AATCATTTGC AAAACACAAT CTAAGTAATC CTGATGGTCT TAATAATTAC 
AGTGCATGTG AGCTGACCTA TGGAATGAAG ACCATAGCAC CACATGGGTA CACTGGATCA 
GTTAAAGTTA GTAATTCCTC TTCAGAAAAA ACATACAGAG TTACATCACC ATCTAGTGGA 
TATTCAAGTC AGTCAAACAC TCCCACCACA TTGACACCAG TGCCTCTTTT CTCAAAGAAT 
ATATATCCAG AAAATGGAAA ATACAAGTTA AAACCAAAAG TACCTGAAAG ACGATCTTCA 
TTGGTATCCT CGGTTTCAGT TTCATCTTCC TCTACTTCCC TTTCTTCAAA TACATTGGAC 
TCTCTGCAGC AGAACATGCA AATTTCAACT GTTATGTTGT CAGCCCATCA CACTCTTCCA 
ACCCCACAAC TCACTCCTGA CATGCCCCCA CCCCCAGCAA TGAATGCAAC CAACACAATT 
TTTGTCGAGT GTTCACCAAA ATGTCCACCT CCTTCCCTTG AATTTACAAG GCTAACAGCG 
GATAGCATGG ATATGTGGCT TCCCTTCTCT CAAAACACTG CCATGAGTAA ACCTAAGGAC 
AGTCTTCCAT ATCCTTCCTT TCCACCTTCT CTTTCCATGA TGCACAGCAT GGTCCCATCA 
CCTGTACCTC CACTTAACAT TGAAATGCTG AAAATTGAAA CAGAATCATT TCCTCTCAGA 
AATTCTGAAA GCAGTTATTT TGTACATAAT GATCACAGTA AACATGAACT GTTGATACCA 
ACTGTTCCTA TAGTTACTTC TCATGCACTA GAAATGGTTC ATTTGAGATC TGTGAAAAAA 
GCATCACAAC TGGAAACTAA AACATTAAGA GAACACATGT CAGAATCCCA CCTACAGGAT 
GAGACATCCG CCTTATTGGT ACAGCCATGT TTAGTACCAG CTGCTTCTCC AGAACTAAAT 
TCTAGTGAAA GCCAAATAGA AAATTTCAGC ATTCCTGCCC AGCAAGTCTC TGGTTTTAAA 
AGCAATGCAG TGAAACTAAC TATTGGTGAA ATAGAAGCAG ACAAGGCATA TAAAGAGCAC 
ATAGAAAAAA ATACAAATTC AGCATACAAT GGAAACTCAT CAGGGCCCAA ATTATCTCCT 
CATGAGAAAC CAAGTATGTC ACAAAGCACA CCAAATTCAT CTCTTAGCAA GGAGCCGCCT 
CTTGTTTCAA AGAAGCCTAA ACTTGTACTT ATTGCCCCAA CGCCACAACT TGATTTGGCA 
GCAGAAAAAA TAGCTCAAGT CACTGAAAAT GTGAGAAGCA TGCCAAATGC CTTTGAAAAA 
GATTCAGTTA GCAAATGTTG TGTGGGGGAG ATAAATGACA GAACTAAAGA TGAAGACATA 
GCAGGTCCAA CCAATGGTTT GGCTCACCAA GAACAAGAAG CCTGGTTATC CCCATGCAAA 
GCTGCTAAAA ATATGATTTT CATACTACCA TCAGTTCCAG AAATTCAGAA TCATCAGCAA 
AAGGAAGAGC AGCAATCCAT GTCAGATGAA TTCAACATTA GTGGTAACAT TTCTGAGACT 
GAGAATACAA GCTGCAACCA GAATGCATCT CAAGATGATG AGAGTGCTGA AGTGTTTAAT 
ACAGAGACGG TGAATTCAGT TCTCTGGACA AGCAGCGACA TTATGGGAAA TGTAGAAGAG 
ACCACTCCAA CAAGGCCTGC TACAACTGAG GATCTTTTTG CTCGTATCCA TAGATCCAAA 
AAAAAAATTC TTGGACGCAA AGATTCTGAC GATGATCGTT GCAGAGATCA CTCGCCTTCT 
TCTCCAGTTA CCCCAACTGG TAATATGCAT CTAAGTTCCT TCAAGCAGAC AGGTTCAATC 
CAAAGAAGTG TCAGAAAAAC TACAACAAGT AGTGACAGTT TCAAAGCACT GCTGTTAAAA 
AAGGGAAGCA GGTCTGAAAG TGGCTTCCGC ATGTCTGCTG CAGAAATGCT GAAACATACT 
GATCCAAGGT TTCAGAGATC AAGATCAGAA TCATCTCTGG AGTTTCCAGA AAGCCCCACA 
TTGTCATCAC CAAACAAGAA CAAGAAGGAG CAACAGGAAT GGGCAAAAAG TGAAGGGCTG 
ATGCCTAGAA GTATGTCTGG CTCTGTTGTC CGGTGCAGCC GCGCCAAGAC TCCCCCTTCT 
GCATCTAGTA GCAAATACAA TGTCCGTAGT CGTCTCCAGA GTAGCCCAAT GACTGTTATA 
TGTGAAGGAG AAGGAGAGGG TTCAGAAACA GTGGATAGCA GCTGTTCAAA GGGTCCTCTT 
TTGTTGAGCA CAAAGAGTCT CGATATCTGT TACAGTGCTG ATAAAGAAGT GACCAACCAC 
ATACTGCACA TAAACTTTAA CACACTATTA GAAAAGAGCA CAGATGTTGC TAGTGGCTGC 
ACAGGGGAAA TAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_012962802.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818256.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X3, mRNA.

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CloneID	OXa32637
Clone ID Related Accession (Same CDS sequence)	XM_012962802.2 , XM_012962802.3
Accession Version	XM_012962802.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962802.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTATAT TCGTTAATGC AATGCTGAAA TCATTTATTA GTATGTTCAA GAAAAAAACG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT 
GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC 
CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC 
AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG 
TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT 
CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA 
AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT 
ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC 
AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA 
GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA 
AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA 
AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818256.1
CDS	294..4979
Translation	MVIFVNAMLKSFISMFKKKTVSNLDEESKWTVHYTASCHQQENVFLSSSRPLCVDDLHRQAKVNLKTVLRECDKLRKDGCRSSQYYSQGPTFSTLRLDCTSYQEEENTHESKFSVSFNEEEQILSTKRPKTPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHRSKKKILGRKDSDDDRCRDHSPSSPVTPTGNMHLSSFKQTGSIQRSVRKTTTSSDSFKALLLKKGSRSESGFRMSAAEMLKHTDPRFQRSRSESSLEFPESPTLSSPNKNKKEQQEWAKSEGLMPRSMSGSVVRCSRAKTPPSASSSKYNVRSRLQSSPMTVICEGEGEGSETVDSSCSKGPLLLSTKSLDICYSADKEVTNHILHINFNTLLEKSTDVASGCTGEII

Target ORF information:

RefSeq Version	XM_012962802.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962802.3

ORF Insert Sequence:

ATGGTTATAT TCGTTAATGC AATGCTGAAA TCATTTATTA GTATGTTCAA GAAAAAAACG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT 
GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC 
CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC 
AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG 
TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT 
CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA 
AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT 
ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC 
AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA 
GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA 
AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA 
AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( NM_001142125.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001135597.1 Xenopus tropicalis NHS like 1 (nhsl1), mRNA.

Price & Availability↑

CloneID	OXa21753
Clone ID Related Accession (Same CDS sequence)	NM_001142125.1
Accession Version	NM_001142125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168125.1. ##Evidence-Data-START## Transcript exon combination :: BC168125.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTGCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCGAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGATAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT 
GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC 
CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC 
AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG 
TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT 
CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA 
AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT 
ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC 
AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA 
GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA 
AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA 
AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135597.1
CDS	252..5177
Translation	MPFHLRLVQPIYVCRLGDPDAQAPRGGNYLIFNSLEDVSSYSLRQIVRQLSDLCRHAENIFSSIHFEASSLGDRTTRLKHRISFLQHNISHLDHKRVTVPVSNLDEESKWTVHYTASCHQQENVFLSSSRPLCVDDLHRQAKVNLKTVLRECDKLRKDGCRSSQYYSQGPTFSTLRLDCTSYQEEENTHESKFSVAFNEEEQILSTKRPETPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLIQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHRSKKKILGRKDSDDDRCRDHSPSSPVTPTGNMHLSSFKQTGSIQRSVRKTTTSSDSFKALLLKKGSRSESGFRMSAAEMLKHTDPRFQRSRSESSLEFPESPTLSSPNKNKKEQQEWAKSEGLMPRSMSGSVVRCSRAKTPPSASSSKYNVRSRLQSSPMTVICEGEGEGSETVDSSCSKGPLLLSTKSLDICYSADKEVTNHILHINFNTLLEKSTDVASGCTGEII

Target ORF information:

RefSeq Version	NM_001142125.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142125.1

ORF Insert Sequence:

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTGCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCGAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGATAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT 
GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC 
CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC 
AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG 
TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT 
CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA 
AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT 
ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC 
AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA 
GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA 
AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA 
AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_012962800.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818254.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa32635
Clone ID Related Accession (Same CDS sequence)	XM_012962800.2
Accession Version	XM_012962800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGCAGGTAC TGGACACTCT 
GACCTAAGAA GGAATTCAGT GCTCATACCT GATGATTTTA ATACTTTGGA ACATTGTCAC 
CACTCAATGC AATTTCTGGA CACCAGAGAT TTTGGCTGCC AAACCAATAA AATAAAAATA 
GTGCCACCAT CAATTAGAAG AATCAGAGCA CAGAAAGGGC AAGGAATTGG AATTCTGATG 
TCCACTTCCT CTGGCAACAT GTCTACTCTA AGTGACTCTG CAGCTTGTTC TTCATATCTA 
AATGGTAACC TGCACTTCCG TAGTCTGCCC AGGATTGGTG CAAGAGAATC TCTTCAATCC 
CTTAACCAAA AAAATTATTA TTACAGCAAC ATGGAGGACC CTTTTACAAC TTTGCCTTGG 
CAGTTAAACA AACTGCAAGT GGATGATACT AATGTTCAAT TAAGGAGTAA CCCTAGGTCA 
ACTATTAATC AAAGGCCTGA GTCTCAAGAA GTAAAAAATT CTACTAGTGA AATTGCCTCA 
AGTCCAGCAT GTATGGTCTC GCCTCATTCT TCTTATTCAA CAAACATTAT TCCAAATGCC 
ATACTTTCAT ATTCCTCAGA AGTAATAAGT ATTCACACTC CCCACAGAGG TGGACTTCTG 
GACAAGAAAC CAAGAGTCCA CAGTTCATCT TCTAACAGAA ATGGTATTAG CAGGGACTAT 
ACTTCTGGCA TTAAAGATAA TTCCTCACCT GGTAACTGGC GTGAAAACAG TTCATCTAGG 
AACTCACAGA TATCAGATAC AAAGTCACCT ATCAGTAAAA AAAGAGATGT TCCACACAAC 
GTTCCTTGCA AAATTAACAC TGGCTTTAAC AACTACTCAT TTGACAGTGA CAATCAAAGT 
GAATTAAGCT ACTCAGATGG TCGTCAAAGA AATGGTAGTA TAACTAGCAG CATTAATAGT 
GCTGACAACT GGTTGTATGA ATCTAGGCAC AATGACGGTG CTCCATCTAG GAAACTATCT 
TCTGTAATTT CTGGGTCTCC TGTTAGCAAT ATGAGCGCCT GCAGTTCTAA GGGGAAGGTA 
GATTCCAGCT CTTTGTATTT TCTGAACCAT GATGATTTTT GCACATCAAT GCATATAGAT 
TCTGGAATTA GTTTGACGGA CCAAAGCATT CAGAATCTAC ACTATAATAT AAATATCTTT 
GAAACAAAAA AAGCTGCAAA TATAGAAGTG AAAACAGGAT ATAGTGACAA ATCACTTCCA 
CGTAGCATAT CTTTAAAGAA ATCTAAAAAT CCTCCCCCCC CACCTTCCAG GACAGACTCA 
CTTAGAAGGC CATCTAAGAA AGATATTCAG ACCAGTGGGC AGGTGCTCAG TGAAACTCTT 
ATTGCTACGC TTCAACAGTC CTTGCAACTA AACTTGAAGA GTAAGAGCAC TGGGTCCACT 
TTGCAGTCAA GTCACTGTAT GGATTATGCA GATCCCTGCA TCATGTGCTC ATGTAGCCAG 
AGTACACTTA GCAGCACTAG TAGTGGAAAA TCTGTTATTG CTCCAAATAT GTACACTCTC 
TGTACTGTGA CACCCTCTCA GAGTGAAACC AGTAGCATGA AATCTGAATA TGCGGATCAG 
TGGAGTTCCT TTATTGACTG TTCACAGACT CCAAAAGAGC AACCCAAATC ATTTGCAAAA 
CACAATCTAA GTAATCCTGA TGGTCTTAAT AATTACAGTG CATGTGAGCT GACCTATGGA 
ATGAAGACCA TAGCACCACA TGGGTACACT GGATCAGTTA AAGTTAGTAA TTCCTCTTCA 
GAAAAAACAT ACAGAGTTAC ATCACCATCT AGTGGATATT CAAGTCAGTC AAACACTCCC 
ACCACATTGA CACCAGTGCC TCTTTTCTCA AAGAATATAT ATCCAGAAAA TGGAAAATAC 
AAGTTAAAAC CAAAAGTACC TGAAAGACGA TCTTCATTGG TATCCTCGGT TTCAGTTTCA 
TCTTCCTCTA CTTCCCTTTC TTCAAATACA TTGGACTCTC TGCAGCAGAA CATGCAAATT 
TCAACTGTTA TGTTGTCAGC CCATCACACT CTTCCAACCC CACAACTCAC TCCTGACATG 
CCCCCACCCC CAGCAATGAA TGCAACCAAC ACAATTTTTG TCGAGTGTTC ACCAAAATGT 
CCACCTCCTT CCCTTGAATT TACAAGGCTA ACAGCGGATA GCATGGATAT GTGGCTTCCC 
TTCTCTCAAA ACACTGCCAT GAGTAAACCT AAGGACAGTC TTCCATATCC TTCCTTTCCA 
CCTTCTCTTT CCATGATGCA CAGCATGGTC CCATCACCTG TACCTCCACT TAACATTGAA 
ATGCTGAAAA TTGAAACAGA ATCATTTCCT CTCAGAAATT CTGAAAGCAG TTATTTTGTA 
CATAATGATC ACAGTAAACA TGAACTGTTG ATACCAACTG TTCCTATAGT TACTTCTCAT 
GCACTAGAAA TGGTTCATTT GAGATCTGTG AAAAAAGCAT CACAACTGGA AACTAAAACA 
TTAAGAGAAC ACATGTCAGA ATCCCACCTA CAGGATGAGA CATCCGCCTT ATTGGTACAG 
CCATGTTTAG TACCAGCTGC TTCTCCAGAA CTAAATTCTA GTGAAAGCCA AATAGAAAAT 
TTCAGCATTC CTGCCCAGCA AGTCTCTGGT TTTAAAAGCA ATGCAGTGAA ACTAACTATT 
GGTGAAATAG AAGCAGACAA GGCATATAAA GAGCACATAG AAAAAAATAC AAATTCAGCA 
TACAATGGAA ACTCATCAGG GCCCAAATTA TCTCCTCATG AGAAACCAAG TATGTCACAA 
AGCACACCAA ATTCATCTCT TAGCAAGGAG CCGCCTCTTG TTTCAAAGAA GCCTAAACTT 
GTACTTATTG CCCCAACGCC ACAACTTGAT TTGGCAGCAG AAAAAATAGC TCAAGTCACT 
GAAAATGTGA GAAGCATGCC AAATGCCTTT GAAAAAGATT CAGTTAGCAA ATGTTGTGTG 
GGGGAGATAA ATGACAGAAC TAAAGATGAA GACATAGCAG GTCCAACCAA TGGTTTGGCT 
CACCAAGAAC AAGAAGCCTG GTTATCCCCA TGCAAAGCTG CTAAAAATAT GATTTTCATA 
CTACCATCAG TTCCAGAAAT TCAGAATCAT CAGCAAAAGG AAGAGCAGCA ATCCATGTCA 
GATGAATTCA ACATTAGTGG TAACATTTCT GAGACTGAGA ATACAAGCTG CAACCAGAAT 
GCATCTCAAG ATGATGAGAG TGCTGAAGTG TTTAATACAG AGACGGTGAA TTCAGTTCTC 
TGGACAAGCA GCGACATTAT GGGAAATGTA GAAGAGACCA CTCCAACAAG GCCTGCTACA 
ACTGAGGATC TTTTTGCTCG TATCCATAGA TCCAAAAAAA AAATTCTTGG ACGCAAAGAT 
TCTGACGATG ATCGTTGCAG AGATCACTCG CCTTCTTCTC CAGTTACCCC AACTGGTAAT 
ATGCATCTAA GTTCCTTCAA GCAGACAGGT TCAATCCAAA GAAGTGTCAG AAAAACTACA 
ACAAGTAGTG ACAGTTTCAA AGCACTGCTG TTAAAAAAGG GAAGCAGGTC TGAAAGTGGC 
TTCCGCATGT CTGCTGCAGA AATGCTGAAA CATACTGATC CAAGGTTTCA GAGATCAAGA 
TCAGAATCAT CTCTGGAGTT TCCAGAAAGC CCCACATTGT CATCACCAAA CAAGAACAAG 
AAGGAGCAAC AGGAATGGGC AAAAAGTGAA GGGCTGATGC CTAGAAGTAT GTCTGGCTCT 
GTTGTCCGGT GCAGCCGCGC CAAGACTCCC CCTTCTGCAT CTAGTAGCAA ATACAATGTC 
CGTAGTCGTC TCCAGAGTAG CCCAATGACT GTTATATGTG AAGGAGAAGG AGAGGGTTCA 
GAAACAGTGG ATAGCAGCTG TTCAAAGGGT CCTCTTTTGT TGAGCACAAA GAGTCTCGAT 
ATCTGTTACA GTGCTGATAA AGAAGTGACC AACCACATAC TGCACATAAA CTTTAACACA 
CTATTAGAAA AGAGCACAGA TGTTGCTAGT GGCTGCACAG GGGAAATAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818254.1
CDS	636..5429
Translation	MPFHLRLVQPIYVCRLGDPDAQAPRGGNYLIFNSLEDVSSYSLRQIVRQLSDLCRHAENIFSSIHFEASSLGDRTTRLKHRISFLQHNISHLDHKRVTVPVSNLDEESKWTVHYTASCHQQENVFLSSSRPLCVDDLHRQAKVNLKTVLRECDKLRKDGCRSSQYYSQGPTFSTLRLDCTSYQEEENTHESKFSVSFNEEEQILSTKRPKTPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHRSKKKILGRKDSDDDRCRDHSPSSPVTPTGNMHLSSFKQTGSIQRSVRKTTTSSDSFKALLLKKGSRSESGFRMSAAEMLKHTDPRFQRSRSESSLEFPESPTLSSPNKNKKEQQEWAKSEGLMPRSMSGSVVRCSRAKTPPSASSSKYNVRSRLQSSPMTVICEGEGEGSETVDSSCSKGPLLLSTKSLDICYSADKEVTNHILHINFNTLLEKSTDVASGCTGEII

Target ORF information:

RefSeq Version	XM_012962800.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962800.2

ORF Insert Sequence:

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGCAGGTAC TGGACACTCT 
GACCTAAGAA GGAATTCAGT GCTCATACCT GATGATTTTA ATACTTTGGA ACATTGTCAC 
CACTCAATGC AATTTCTGGA CACCAGAGAT TTTGGCTGCC AAACCAATAA AATAAAAATA 
GTGCCACCAT CAATTAGAAG AATCAGAGCA CAGAAAGGGC AAGGAATTGG AATTCTGATG 
TCCACTTCCT CTGGCAACAT GTCTACTCTA AGTGACTCTG CAGCTTGTTC TTCATATCTA 
AATGGTAACC TGCACTTCCG TAGTCTGCCC AGGATTGGTG CAAGAGAATC TCTTCAATCC 
CTTAACCAAA AAAATTATTA TTACAGCAAC ATGGAGGACC CTTTTACAAC TTTGCCTTGG 
CAGTTAAACA AACTGCAAGT GGATGATACT AATGTTCAAT TAAGGAGTAA CCCTAGGTCA 
ACTATTAATC AAAGGCCTGA GTCTCAAGAA GTAAAAAATT CTACTAGTGA AATTGCCTCA 
AGTCCAGCAT GTATGGTCTC GCCTCATTCT TCTTATTCAA CAAACATTAT TCCAAATGCC 
ATACTTTCAT ATTCCTCAGA AGTAATAAGT ATTCACACTC CCCACAGAGG TGGACTTCTG 
GACAAGAAAC CAAGAGTCCA CAGTTCATCT TCTAACAGAA ATGGTATTAG CAGGGACTAT 
ACTTCTGGCA TTAAAGATAA TTCCTCACCT GGTAACTGGC GTGAAAACAG TTCATCTAGG 
AACTCACAGA TATCAGATAC AAAGTCACCT ATCAGTAAAA AAAGAGATGT TCCACACAAC 
GTTCCTTGCA AAATTAACAC TGGCTTTAAC AACTACTCAT TTGACAGTGA CAATCAAAGT 
GAATTAAGCT ACTCAGATGG TCGTCAAAGA AATGGTAGTA TAACTAGCAG CATTAATAGT 
GCTGACAACT GGTTGTATGA ATCTAGGCAC AATGACGGTG CTCCATCTAG GAAACTATCT 
TCTGTAATTT CTGGGTCTCC TGTTAGCAAT ATGAGCGCCT GCAGTTCTAA GGGGAAGGTA 
GATTCCAGCT CTTTGTATTT TCTGAACCAT GATGATTTTT GCACATCAAT GCATATAGAT 
TCTGGAATTA GTTTGACGGA CCAAAGCATT CAGAATCTAC ACTATAATAT AAATATCTTT 
GAAACAAAAA AAGCTGCAAA TATAGAAGTG AAAACAGGAT ATAGTGACAA ATCACTTCCA 
CGTAGCATAT CTTTAAAGAA ATCTAAAAAT CCTCCCCCCC CACCTTCCAG GACAGACTCA 
CTTAGAAGGC CATCTAAGAA AGATATTCAG ACCAGTGGGC AGGTGCTCAG TGAAACTCTT 
ATTGCTACGC TTCAACAGTC CTTGCAACTA AACTTGAAGA GTAAGAGCAC TGGGTCCACT 
TTGCAGTCAA GTCACTGTAT GGATTATGCA GATCCCTGCA TCATGTGCTC ATGTAGCCAG 
AGTACACTTA GCAGCACTAG TAGTGGAAAA TCTGTTATTG CTCCAAATAT GTACACTCTC 
TGTACTGTGA CACCCTCTCA GAGTGAAACC AGTAGCATGA AATCTGAATA TGCGGATCAG 
TGGAGTTCCT TTATTGACTG TTCACAGACT CCAAAAGAGC AACCCAAATC ATTTGCAAAA 
CACAATCTAA GTAATCCTGA TGGTCTTAAT AATTACAGTG CATGTGAGCT GACCTATGGA 
ATGAAGACCA TAGCACCACA TGGGTACACT GGATCAGTTA AAGTTAGTAA TTCCTCTTCA 
GAAAAAACAT ACAGAGTTAC ATCACCATCT AGTGGATATT CAAGTCAGTC AAACACTCCC 
ACCACATTGA CACCAGTGCC TCTTTTCTCA AAGAATATAT ATCCAGAAAA TGGAAAATAC 
AAGTTAAAAC CAAAAGTACC TGAAAGACGA TCTTCATTGG TATCCTCGGT TTCAGTTTCA 
TCTTCCTCTA CTTCCCTTTC TTCAAATACA TTGGACTCTC TGCAGCAGAA CATGCAAATT 
TCAACTGTTA TGTTGTCAGC CCATCACACT CTTCCAACCC CACAACTCAC TCCTGACATG 
CCCCCACCCC CAGCAATGAA TGCAACCAAC ACAATTTTTG TCGAGTGTTC ACCAAAATGT 
CCACCTCCTT CCCTTGAATT TACAAGGCTA ACAGCGGATA GCATGGATAT GTGGCTTCCC 
TTCTCTCAAA ACACTGCCAT GAGTAAACCT AAGGACAGTC TTCCATATCC TTCCTTTCCA 
CCTTCTCTTT CCATGATGCA CAGCATGGTC CCATCACCTG TACCTCCACT TAACATTGAA 
ATGCTGAAAA TTGAAACAGA ATCATTTCCT CTCAGAAATT CTGAAAGCAG TTATTTTGTA 
CATAATGATC ACAGTAAACA TGAACTGTTG ATACCAACTG TTCCTATAGT TACTTCTCAT 
GCACTAGAAA TGGTTCATTT GAGATCTGTG AAAAAAGCAT CACAACTGGA AACTAAAACA 
TTAAGAGAAC ACATGTCAGA ATCCCACCTA CAGGATGAGA CATCCGCCTT ATTGGTACAG 
CCATGTTTAG TACCAGCTGC TTCTCCAGAA CTAAATTCTA GTGAAAGCCA AATAGAAAAT 
TTCAGCATTC CTGCCCAGCA AGTCTCTGGT TTTAAAAGCA ATGCAGTGAA ACTAACTATT 
GGTGAAATAG AAGCAGACAA GGCATATAAA GAGCACATAG AAAAAAATAC AAATTCAGCA 
TACAATGGAA ACTCATCAGG GCCCAAATTA TCTCCTCATG AGAAACCAAG TATGTCACAA 
AGCACACCAA ATTCATCTCT TAGCAAGGAG CCGCCTCTTG TTTCAAAGAA GCCTAAACTT 
GTACTTATTG CCCCAACGCC ACAACTTGAT TTGGCAGCAG AAAAAATAGC TCAAGTCACT 
GAAAATGTGA GAAGCATGCC AAATGCCTTT GAAAAAGATT CAGTTAGCAA ATGTTGTGTG 
GGGGAGATAA ATGACAGAAC TAAAGATGAA GACATAGCAG GTCCAACCAA TGGTTTGGCT 
CACCAAGAAC AAGAAGCCTG GTTATCCCCA TGCAAAGCTG CTAAAAATAT GATTTTCATA 
CTACCATCAG TTCCAGAAAT TCAGAATCAT CAGCAAAAGG AAGAGCAGCA ATCCATGTCA 
GATGAATTCA ACATTAGTGG TAACATTTCT GAGACTGAGA ATACAAGCTG CAACCAGAAT 
GCATCTCAAG ATGATGAGAG TGCTGAAGTG TTTAATACAG AGACGGTGAA TTCAGTTCTC 
TGGACAAGCA GCGACATTAT GGGAAATGTA GAAGAGACCA CTCCAACAAG GCCTGCTACA 
ACTGAGGATC TTTTTGCTCG TATCCATAGA TCCAAAAAAA AAATTCTTGG ACGCAAAGAT 
TCTGACGATG ATCGTTGCAG AGATCACTCG CCTTCTTCTC CAGTTACCCC AACTGGTAAT 
ATGCATCTAA GTTCCTTCAA GCAGACAGGT TCAATCCAAA GAAGTGTCAG AAAAACTACA 
ACAAGTAGTG ACAGTTTCAA AGCACTGCTG TTAAAAAAGG GAAGCAGGTC TGAAAGTGGC 
TTCCGCATGT CTGCTGCAGA AATGCTGAAA CATACTGATC CAAGGTTTCA GAGATCAAGA 
TCAGAATCAT CTCTGGAGTT TCCAGAAAGC CCCACATTGT CATCACCAAA CAAGAACAAG 
AAGGAGCAAC AGGAATGGGC AAAAAGTGAA GGGCTGATGC CTAGAAGTAT GTCTGGCTCT 
GTTGTCCGGT GCAGCCGCGC CAAGACTCCC CCTTCTGCAT CTAGTAGCAA ATACAATGTC 
CGTAGTCGTC TCCAGAGTAG CCCAATGACT GTTATATGTG AAGGAGAAGG AGAGGGTTCA 
GAAACAGTGG ATAGCAGCTG TTCAAAGGGT CCTCTTTTGT TGAGCACAAA GAGTCTCGAT 
ATCTGTTACA GTGCTGATAA AGAAGTGACC AACCACATAC TGCACATAAA CTTTAACACA 
CTATTAGAAA AGAGCACAGA TGTTGCTAGT GGCTGCACAG GGGAAATAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_012962801.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818255.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X2, mRNA.

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CloneID	OXa32636
Clone ID Related Accession (Same CDS sequence)	XM_012962801.2
Accession Version	XM_012962801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962801.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCAA 
TGTGACAAGC TTCGGAAGGA TGGCTGTCGC AGTTCACAGT ACTACTCCCA GGGACCAACA 
TTCTCGACAC TCAGACTGGA TTGCACAAGC TATCAGGAAG AGGAAAATAC ACATGAGTCA 
AAGTTTTCTG TTTCATTTAA TGAAGAGGAA CAGATCCTTT CAACCAAAAG GCCCAAGACT 
CCTGTGTTGC TTGAGTTTTC TGAAACGCTC ACTAACTGGA CCAAGTCACT GCCACTGCCT 
ACTCCAGAGG AAAAGATGCG TCAGGAGGCT CAGGCAGTAC AGACAGATGT AATTCCTATA 
AACATAACAG GGGAGAATTT CAACCGCCAG GCCAGCTTTC AGCGGTCACT TATTTATACA 
GACACTGCCG TAAGATGTTC GAAGAAAGTC AAAAGGAGAA AGACTATAAC AGGCGTCCCA 
AACAACTTAC AGAAGGAGCT AGCAGGTACT GGACACTCTG ACCTAAGAAG GAATTCAGTG 
CTCATACCTG ATGATTTTAA TACTTTGGAA CATTGTCACC ACTCAATGCA ATTTCTGGAC 
ACCAGAGATT TTGGCTGCCA AACCAATAAA ATAAAAATAG TGCCACCATC AATTAGAAGA 
ATCAGAGCAC AGAAAGGGCA AGGAATTGGA ATTCTGATGT CCACTTCCTC TGGCAACATG 
TCTACTCTAA GTGACTCTGC AGCTTGTTCT TCATATCTAA ATGGTAACCT GCACTTCCGT 
AGTCTGCCCA GGATTGGTGC AAGAGAATCT CTTCAATCCC TTAACCAAAA AAATTATTAT 
TACAGCAACA TGGAGGACCC TTTTACAACT TTGCCTTGGC AGTTAAACAA ACTGCAAGTG 
GATGATACTA ATGTTCAATT AAGGAGTAAC CCTAGGTCAA CTATTAATCA AAGGCCTGAG 
TCTCAAGAAG TAAAAAATTC TACTAGTGAA ATTGCCTCAA GTCCAGCATG TATGGTCTCG 
CCTCATTCTT CTTATTCAAC AAACATTATT CCAAATGCCA TACTTTCATA TTCCTCAGAA 
GTAATAAGTA TTCACACTCC CCACAGAGGT GGACTTCTGG ACAAGAAACC AAGAGTCCAC 
AGTTCATCTT CTAACAGAAA TGGTATTAGC AGGGACTATA CTTCTGGCAT TAAAGATAAT 
TCCTCACCTG GTAACTGGCG TGAAAACAGT TCATCTAGGA ACTCACAGAT ATCAGATACA 
AAGTCACCTA TCAGTAAAAA AAGAGATGTT CCACACAACG TTCCTTGCAA AATTAACACT 
GGCTTTAACA ACTACTCATT TGACAGTGAC AATCAAAGTG AATTAAGCTA CTCAGATGGT 
CGTCAAAGAA ATGGTAGTAT AACTAGCAGC ATTAATAGTG CTGACAACTG GTTGTATGAA 
TCTAGGCACA ATGACGGTGC TCCATCTAGG AAACTATCTT CTGTAATTTC TGGGTCTCCT 
GTTAGCAATA TGAGCGCCTG CAGTTCTAAG GGGAAGGTAG ATTCCAGCTC TTTGTATTTT 
CTGAACCATG ATGATTTTTG CACATCAATG CATATAGATT CTGGAATTAG TTTGACGGAC 
CAAAGCATTC AGAATCTACA CTATAATATA AATATCTTTG AAACAAAAAA AGCTGCAAAT 
ATAGAAGTGA AAACAGGATA TAGTGACAAA TCACTTCCAC GTAGCATATC TTTAAAGAAA 
TCTAAAAATC CTCCCCCCCC ACCTTCCAGG ACAGACTCAC TTAGAAGGCC ATCTAAGAAA 
GATATTCAGA CCAGTGGGCA GGTGCTCAGT GAAACTCTTA TTGCTACGCT TCAACAGTCC 
TTGCAACTAA ACTTGAAGAG TAAGAGCACT GGGTCCACTT TGCAGTCAAG TCACTGTATG 
GATTATGCAG ATCCCTGCAT CATGTGCTCA TGTAGCCAGA GTACACTTAG CAGCACTAGT 
AGTGGAAAAT CTGTTATTGC TCCAAATATG TACACTCTCT GTACTGTGAC ACCCTCTCAG 
AGTGAAACCA GTAGCATGAA ATCTGAATAT GCGGATCAGT GGAGTTCCTT TATTGACTGT 
TCACAGACTC CAAAAGAGCA ACCCAAATCA TTTGCAAAAC ACAATCTAAG TAATCCTGAT 
GGTCTTAATA ATTACAGTGC ATGTGAGCTG ACCTATGGAA TGAAGACCAT AGCACCACAT 
GGGTACACTG GATCAGTTAA AGTTAGTAAT TCCTCTTCAG AAAAAACATA CAGAGTTACA 
TCACCATCTA GTGGATATTC AAGTCAGTCA AACACTCCCA CCACATTGAC ACCAGTGCCT 
CTTTTCTCAA AGAATATATA TCCAGAAAAT GGAAAATACA AGTTAAAACC AAAAGTACCT 
GAAAGACGAT CTTCATTGGT ATCCTCGGTT TCAGTTTCAT CTTCCTCTAC TTCCCTTTCT 
TCAAATACAT TGGACTCTCT GCAGCAGAAC ATGCAAATTT CAACTGTTAT GTTGTCAGCC 
CATCACACTC TTCCAACCCC ACAACTCACT CCTGACATGC CCCCACCCCC AGCAATGAAT 
GCAACCAACA CAATTTTTGT CGAGTGTTCA CCAAAATGTC CACCTCCTTC CCTTGAATTT 
ACAAGGCTAA CAGCGGATAG CATGGATATG TGGCTTCCCT TCTCTCAAAA CACTGCCATG 
AGTAAACCTA AGGACAGTCT TCCATATCCT TCCTTTCCAC CTTCTCTTTC CATGATGCAC 
AGCATGGTCC CATCACCTGT ACCTCCACTT AACATTGAAA TGCTGAAAAT TGAAACAGAA 
TCATTTCCTC TCAGAAATTC TGAAAGCAGT TATTTTGTAC ATAATGATCA CAGTAAACAT 
GAACTGTTGA TACCAACTGT TCCTATAGTT ACTTCTCATG CACTAGAAAT GGTTCATTTG 
AGATCTGTGA AAAAAGCATC ACAACTGGAA ACTAAAACAT TAAGAGAACA CATGTCAGAA 
TCCCACCTAC AGGATGAGAC ATCCGCCTTA TTGGTACAGC CATGTTTAGT ACCAGCTGCT 
TCTCCAGAAC TAAATTCTAG TGAAAGCCAA ATAGAAAATT TCAGCATTCC TGCCCAGCAA 
GTCTCTGGTT TTAAAAGCAA TGCAGTGAAA CTAACTATTG GTGAAATAGA AGCAGACAAG 
GCATATAAAG AGCACATAGA AAAAAATACA AATTCAGCAT ACAATGGAAA CTCATCAGGG 
CCCAAATTAT CTCCTCATGA GAAACCAAGT ATGTCACAAA GCACACCAAA TTCATCTCTT 
AGCAAGGAGC CGCCTCTTGT TTCAAAGAAG CCTAAACTTG TACTTATTGC CCCAACGCCA 
CAACTTGATT TGGCAGCAGA AAAAATAGCT CAAGTCACTG AAAATGTGAG AAGCATGCCA 
AATGCCTTTG AAAAAGATTC AGTTAGCAAA TGTTGTGTGG GGGAGATAAA TGACAGAACT 
AAAGATGAAG ACATAGCAGG TCCAACCAAT GGTTTGGCTC ACCAAGAACA AGAAGCCTGG 
TTATCCCCAT GCAAAGCTGC TAAAAATATG ATTTTCATAC TACCATCAGT TCCAGAAATT 
CAGAATCATC AGCAAAAGGA AGAGCAGCAA TCCATGTCAG ATGAATTCAA CATTAGTGGT 
AACATTTCTG AGACTGAGAA TACAAGCTGC AACCAGAATG CATCTCAAGA TGATGAGAGT 
GCTGAAGTGT TTAATACAGA GACGGTGAAT TCAGTTCTCT GGACAAGCAG CGACATTATG 
GGAAATGTAG AAGAGACCAC TCCAACAAGG CCTGCTACAA CTGAGGATCT TTTTGCTCGT 
ATCCATAGAT CCAAAAAAAA AATTCTTGGA CGCAAAGATT CTGACGATGA TCGTTGCAGA 
GATCACTCGC CTTCTTCTCC AGTTACCCCA ACTGGTAATA TGCATCTAAG TTCCTTCAAG 
CAGACAGGTT CAATCCAAAG AAGTGTCAGA AAAACTACAA CAAGTAGTGA CAGTTTCAAA 
GCACTGCTGT TAAAAAAGGG AAGCAGGTCT GAAAGTGGCT TCCGCATGTC TGCTGCAGAA 
ATGCTGAAAC ATACTGATCC AAGGTTTCAG AGATCAAGAT CAGAATCATC TCTGGAGTTT 
CCAGAAAGCC CCACATTGTC ATCACCAAAC AAGAACAAGA AGGAGCAACA GGAATGGGCA 
AAAAGTGAAG GGCTGATGCC TAGAAGTATG TCTGGCTCTG TTGTCCGGTG CAGCCGCGCC 
AAGACTCCCC CTTCTGCATC TAGTAGCAAA TACAATGTCC GTAGTCGTCT CCAGAGTAGC 
CCAATGACTG TTATATGTGA AGGAGAAGGA GAGGGTTCAG AAACAGTGGA TAGCAGCTGT 
TCAAAGGGTC CTCTTTTGTT GAGCACAAAG AGTCTCGATA TCTGTTACAG TGCTGATAAA 
GAAGTGACCA ACCACATACT GCACATAAAC TTTAACACAC TATTAGAAAA GAGCACAGAT 
GTTGCTAGTG GCTGCACAGG GGAAATAATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818255.1
CDS	636..5408
Translation	MPFHLRLVQPIYVCRLGDPDAQAPRGGNYLIFNSLEDVSSYSLRQIVRQLSDLCRHAENIFSSIHFEASSLGDRTTRLKHRISFLQHNISHLDHKRVTVQCDKLRKDGCRSSQYYSQGPTFSTLRLDCTSYQEEENTHESKFSVSFNEEEQILSTKRPKTPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHRSKKKILGRKDSDDDRCRDHSPSSPVTPTGNMHLSSFKQTGSIQRSVRKTTTSSDSFKALLLKKGSRSESGFRMSAAEMLKHTDPRFQRSRSESSLEFPESPTLSSPNKNKKEQQEWAKSEGLMPRSMSGSVVRCSRAKTPPSASSSKYNVRSRLQSSPMTVICEGEGEGSETVDSSCSKGPLLLSTKSLDICYSADKEVTNHILHINFNTLLEKSTDVASGCTGEII

Target ORF information:

RefSeq Version	XM_012962801.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962801.2

ORF Insert Sequence:

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCAA 
TGTGACAAGC TTCGGAAGGA TGGCTGTCGC AGTTCACAGT ACTACTCCCA GGGACCAACA 
TTCTCGACAC TCAGACTGGA TTGCACAAGC TATCAGGAAG AGGAAAATAC ACATGAGTCA 
AAGTTTTCTG TTTCATTTAA TGAAGAGGAA CAGATCCTTT CAACCAAAAG GCCCAAGACT 
CCTGTGTTGC TTGAGTTTTC TGAAACGCTC ACTAACTGGA CCAAGTCACT GCCACTGCCT 
ACTCCAGAGG AAAAGATGCG TCAGGAGGCT CAGGCAGTAC AGACAGATGT AATTCCTATA 
AACATAACAG GGGAGAATTT CAACCGCCAG GCCAGCTTTC AGCGGTCACT TATTTATACA 
GACACTGCCG TAAGATGTTC GAAGAAAGTC AAAAGGAGAA AGACTATAAC AGGCGTCCCA 
AACAACTTAC AGAAGGAGCT AGCAGGTACT GGACACTCTG ACCTAAGAAG GAATTCAGTG 
CTCATACCTG ATGATTTTAA TACTTTGGAA CATTGTCACC ACTCAATGCA ATTTCTGGAC 
ACCAGAGATT TTGGCTGCCA AACCAATAAA ATAAAAATAG TGCCACCATC AATTAGAAGA 
ATCAGAGCAC AGAAAGGGCA AGGAATTGGA ATTCTGATGT CCACTTCCTC TGGCAACATG 
TCTACTCTAA GTGACTCTGC AGCTTGTTCT TCATATCTAA ATGGTAACCT GCACTTCCGT 
AGTCTGCCCA GGATTGGTGC AAGAGAATCT CTTCAATCCC TTAACCAAAA AAATTATTAT 
TACAGCAACA TGGAGGACCC TTTTACAACT TTGCCTTGGC AGTTAAACAA ACTGCAAGTG 
GATGATACTA ATGTTCAATT AAGGAGTAAC CCTAGGTCAA CTATTAATCA AAGGCCTGAG 
TCTCAAGAAG TAAAAAATTC TACTAGTGAA ATTGCCTCAA GTCCAGCATG TATGGTCTCG 
CCTCATTCTT CTTATTCAAC AAACATTATT CCAAATGCCA TACTTTCATA TTCCTCAGAA 
GTAATAAGTA TTCACACTCC CCACAGAGGT GGACTTCTGG ACAAGAAACC AAGAGTCCAC 
AGTTCATCTT CTAACAGAAA TGGTATTAGC AGGGACTATA CTTCTGGCAT TAAAGATAAT 
TCCTCACCTG GTAACTGGCG TGAAAACAGT TCATCTAGGA ACTCACAGAT ATCAGATACA 
AAGTCACCTA TCAGTAAAAA AAGAGATGTT CCACACAACG TTCCTTGCAA AATTAACACT 
GGCTTTAACA ACTACTCATT TGACAGTGAC AATCAAAGTG AATTAAGCTA CTCAGATGGT 
CGTCAAAGAA ATGGTAGTAT AACTAGCAGC ATTAATAGTG CTGACAACTG GTTGTATGAA 
TCTAGGCACA ATGACGGTGC TCCATCTAGG AAACTATCTT CTGTAATTTC TGGGTCTCCT 
GTTAGCAATA TGAGCGCCTG CAGTTCTAAG GGGAAGGTAG ATTCCAGCTC TTTGTATTTT 
CTGAACCATG ATGATTTTTG CACATCAATG CATATAGATT CTGGAATTAG TTTGACGGAC 
CAAAGCATTC AGAATCTACA CTATAATATA AATATCTTTG AAACAAAAAA AGCTGCAAAT 
ATAGAAGTGA AAACAGGATA TAGTGACAAA TCACTTCCAC GTAGCATATC TTTAAAGAAA 
TCTAAAAATC CTCCCCCCCC ACCTTCCAGG ACAGACTCAC TTAGAAGGCC ATCTAAGAAA 
GATATTCAGA CCAGTGGGCA GGTGCTCAGT GAAACTCTTA TTGCTACGCT TCAACAGTCC 
TTGCAACTAA ACTTGAAGAG TAAGAGCACT GGGTCCACTT TGCAGTCAAG TCACTGTATG 
GATTATGCAG ATCCCTGCAT CATGTGCTCA TGTAGCCAGA GTACACTTAG CAGCACTAGT 
AGTGGAAAAT CTGTTATTGC TCCAAATATG TACACTCTCT GTACTGTGAC ACCCTCTCAG 
AGTGAAACCA GTAGCATGAA ATCTGAATAT GCGGATCAGT GGAGTTCCTT TATTGACTGT 
TCACAGACTC CAAAAGAGCA ACCCAAATCA TTTGCAAAAC ACAATCTAAG TAATCCTGAT 
GGTCTTAATA ATTACAGTGC ATGTGAGCTG ACCTATGGAA TGAAGACCAT AGCACCACAT 
GGGTACACTG GATCAGTTAA AGTTAGTAAT TCCTCTTCAG AAAAAACATA CAGAGTTACA 
TCACCATCTA GTGGATATTC AAGTCAGTCA AACACTCCCA CCACATTGAC ACCAGTGCCT 
CTTTTCTCAA AGAATATATA TCCAGAAAAT GGAAAATACA AGTTAAAACC AAAAGTACCT 
GAAAGACGAT CTTCATTGGT ATCCTCGGTT TCAGTTTCAT CTTCCTCTAC TTCCCTTTCT 
TCAAATACAT TGGACTCTCT GCAGCAGAAC ATGCAAATTT CAACTGTTAT GTTGTCAGCC 
CATCACACTC TTCCAACCCC ACAACTCACT CCTGACATGC CCCCACCCCC AGCAATGAAT 
GCAACCAACA CAATTTTTGT CGAGTGTTCA CCAAAATGTC CACCTCCTTC CCTTGAATTT 
ACAAGGCTAA CAGCGGATAG CATGGATATG TGGCTTCCCT TCTCTCAAAA CACTGCCATG 
AGTAAACCTA AGGACAGTCT TCCATATCCT TCCTTTCCAC CTTCTCTTTC CATGATGCAC 
AGCATGGTCC CATCACCTGT ACCTCCACTT AACATTGAAA TGCTGAAAAT TGAAACAGAA 
TCATTTCCTC TCAGAAATTC TGAAAGCAGT TATTTTGTAC ATAATGATCA CAGTAAACAT 
GAACTGTTGA TACCAACTGT TCCTATAGTT ACTTCTCATG CACTAGAAAT GGTTCATTTG 
AGATCTGTGA AAAAAGCATC ACAACTGGAA ACTAAAACAT TAAGAGAACA CATGTCAGAA 
TCCCACCTAC AGGATGAGAC ATCCGCCTTA TTGGTACAGC CATGTTTAGT ACCAGCTGCT 
TCTCCAGAAC TAAATTCTAG TGAAAGCCAA ATAGAAAATT TCAGCATTCC TGCCCAGCAA 
GTCTCTGGTT TTAAAAGCAA TGCAGTGAAA CTAACTATTG GTGAAATAGA AGCAGACAAG 
GCATATAAAG AGCACATAGA AAAAAATACA AATTCAGCAT ACAATGGAAA CTCATCAGGG 
CCCAAATTAT CTCCTCATGA GAAACCAAGT ATGTCACAAA GCACACCAAA TTCATCTCTT 
AGCAAGGAGC CGCCTCTTGT TTCAAAGAAG CCTAAACTTG TACTTATTGC CCCAACGCCA 
CAACTTGATT TGGCAGCAGA AAAAATAGCT CAAGTCACTG AAAATGTGAG AAGCATGCCA 
AATGCCTTTG AAAAAGATTC AGTTAGCAAA TGTTGTGTGG GGGAGATAAA TGACAGAACT 
AAAGATGAAG ACATAGCAGG TCCAACCAAT GGTTTGGCTC ACCAAGAACA AGAAGCCTGG 
TTATCCCCAT GCAAAGCTGC TAAAAATATG ATTTTCATAC TACCATCAGT TCCAGAAATT 
CAGAATCATC AGCAAAAGGA AGAGCAGCAA TCCATGTCAG ATGAATTCAA CATTAGTGGT 
AACATTTCTG AGACTGAGAA TACAAGCTGC AACCAGAATG CATCTCAAGA TGATGAGAGT 
GCTGAAGTGT TTAATACAGA GACGGTGAAT TCAGTTCTCT GGACAAGCAG CGACATTATG 
GGAAATGTAG AAGAGACCAC TCCAACAAGG CCTGCTACAA CTGAGGATCT TTTTGCTCGT 
ATCCATAGAT CCAAAAAAAA AATTCTTGGA CGCAAAGATT CTGACGATGA TCGTTGCAGA 
GATCACTCGC CTTCTTCTCC AGTTACCCCA ACTGGTAATA TGCATCTAAG TTCCTTCAAG 
CAGACAGGTT CAATCCAAAG AAGTGTCAGA AAAACTACAA CAAGTAGTGA CAGTTTCAAA 
GCACTGCTGT TAAAAAAGGG AAGCAGGTCT GAAAGTGGCT TCCGCATGTC TGCTGCAGAA 
ATGCTGAAAC ATACTGATCC AAGGTTTCAG AGATCAAGAT CAGAATCATC TCTGGAGTTT 
CCAGAAAGCC CCACATTGTC ATCACCAAAC AAGAACAAGA AGGAGCAACA GGAATGGGCA 
AAAAGTGAAG GGCTGATGCC TAGAAGTATG TCTGGCTCTG TTGTCCGGTG CAGCCGCGCC 
AAGACTCCCC CTTCTGCATC TAGTAGCAAA TACAATGTCC GTAGTCGTCT CCAGAGTAGC 
CCAATGACTG TTATATGTGA AGGAGAAGGA GAGGGTTCAG AAACAGTGGA TAGCAGCTGT 
TCAAAGGGTC CTCTTTTGTT GAGCACAAAG AGTCTCGATA TCTGTTACAG TGCTGATAAA 
GAAGTGACCA ACCACATACT GCACATAAAC TTTAACACAC TATTAGAAAA GAGCACAGAT 
GTTGCTAGTG GCTGCACAGG GGAAATAATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_012962802.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818256.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X3, mRNA.

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CloneID	OXa32637
Clone ID Related Accession (Same CDS sequence)	XM_012962802.2 , XM_012962802.3
Accession Version	XM_012962802.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTATAT TCGTTAATGC AATGCTGAAA TCATTTATTA GTATGTTCAA GAAAAAAACG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT 
GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC 
CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC 
AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG 
TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT 
CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA 
AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT 
ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC 
AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA 
GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA 
AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA 
AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818256.1
CDS	585..5270
Translation	MVIFVNAMLKSFISMFKKKTVSNLDEESKWTVHYTASCHQQENVFLSSSRPLCVDDLHRQAKVNLKTVLRECDKLRKDGCRSSQYYSQGPTFSTLRLDCTSYQEEENTHESKFSVSFNEEEQILSTKRPKTPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHRSKKKILGRKDSDDDRCRDHSPSSPVTPTGNMHLSSFKQTGSIQRSVRKTTTSSDSFKALLLKKGSRSESGFRMSAAEMLKHTDPRFQRSRSESSLEFPESPTLSSPNKNKKEQQEWAKSEGLMPRSMSGSVVRCSRAKTPPSASSSKYNVRSRLQSSPMTVICEGEGEGSETVDSSCSKGPLLLSTKSLDICYSADKEVTNHILHINFNTLLEKSTDVASGCTGEII

Target ORF information:

RefSeq Version	XM_012962802.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962802.2

ORF Insert Sequence:

ATGGTTATAT TCGTTAATGC AATGCTGAAA TCATTTATTA GTATGTTCAA GAAAAAAACG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GATCCAAAAA AAAAATTCTT 
GGACGCAAAG ATTCTGACGA TGATCGTTGC AGAGATCACT CGCCTTCTTC TCCAGTTACC 
CCAACTGGTA ATATGCATCT AAGTTCCTTC AAGCAGACAG GTTCAATCCA AAGAAGTGTC 
AGAAAAACTA CAACAAGTAG TGACAGTTTC AAAGCACTGC TGTTAAAAAA GGGAAGCAGG 
TCTGAAAGTG GCTTCCGCAT GTCTGCTGCA GAAATGCTGA AACATACTGA TCCAAGGTTT 
CAGAGATCAA GATCAGAATC ATCTCTGGAG TTTCCAGAAA GCCCCACATT GTCATCACCA 
AACAAGAACA AGAAGGAGCA ACAGGAATGG GCAAAAAGTG AAGGGCTGAT GCCTAGAAGT 
ATGTCTGGCT CTGTTGTCCG GTGCAGCCGC GCCAAGACTC CCCCTTCTGC ATCTAGTAGC 
AAATACAATG TCCGTAGTCG TCTCCAGAGT AGCCCAATGA CTGTTATATG TGAAGGAGAA 
GGAGAGGGTT CAGAAACAGT GGATAGCAGC TGTTCAAAGG GTCCTCTTTT GTTGAGCACA 
AAGAGTCTCG ATATCTGTTA CAGTGCTGAT AAAGAAGTGA CCAACCACAT ACTGCACATA 
AACTTTAACA CACTATTAGA AAAGAGCACA GATGTTGCTA GTGGCTGCAC AGGGGAAATA 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_012962803.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818257.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X4, mRNA.

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CloneID	OXa32638
Clone ID Related Accession (Same CDS sequence)	XM_012962803.2
Accession Version	XM_012962803.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACA AGCCGGGGGG CTGTCGTGTC GGCCCTGCAA CGCGATCGTC GCAGCAGACG 
CGATCACATC GCGTCTGCTG CTTCCCCAGT GCCGGCCCAA TGCCTAGGGG GACTTCCTGC 
TCCAGGACTG CAGCACCGAG GACCTGGCAA GCTTCAGGAC AGAATGTGAC AAGCTTCGGA 
AGGATGGCTG TCGCAGTTCA CAGTACTACT CCCAGGGACC AACATTCTCG ACACTCAGAC 
TGGATTGCAC AAGCTATCAG GAAGAGGAAA ATACACATGA GTCAAAGTGC AAACCCACCA 
TGGACCCGCG GCCAGCCTTT TTCTGTTTCA TTTAATGAAG AGGAACAGAT CCTTTCAACC 
AAAAGGCCCA AGACTCCTGT GTTGCTTGAG TTTTCTGAAA CGCTCACTAA CTGGACCAAG 
TCACTGCCAC TGCCTACTCC AGAGGAAAAG ATGCGTCAGG AGGCTCAGGC AGTACAGACA 
GATGTAATTC CTATAAACAT AACAGGGGAG AATTTCAACC GCCAGGCCAG CTTTCAGCGG 
TCACTTATTT ATACAGACAC TGCCGTAAGA TGTTCGAAGA AAGTCAAAAG GAGAAAGACT 
ATAACAGGCG TCCCAAACAA CTTACAGAAG GAGCTAGCAG GTACTGGACA CTCTGACCTA 
AGAAGGAATT CAGTGCTCAT ACCTGATGAT TTTAATACTT TGGAACATTG TCACCACTCA 
ATGCAATTTC TGGACACCAG AGATTTTGGC TGCCAAACCA ATAAAATAAA AATAGTGCCA 
CCATCAATTA GAAGAATCAG AGCACAGAAA GGGCAAGGAA TTGGAATTCT GATGTCCACT 
TCCTCTGGCA ACATGTCTAC TCTAAGTGAC TCTGCAGCTT GTTCTTCATA TCTAAATGGT 
AACCTGCACT TCCGTAGTCT GCCCAGGATT GGTGCAAGAG AATCTCTTCA ATCCCTTAAC 
CAAAAAAATT ATTATTACAG CAACATGGAG GACCCTTTTA CAACTTTGCC TTGGCAGTTA 
AACAAACTGC AAGTGGATGA TACTAATGTT CAATTAAGGA GTAACCCTAG GTCAACTATT 
AATCAAAGGC CTGAGTCTCA AGAAGTAAAA AATTCTACTA GTGAAATTGC CTCAAGTCCA 
GCATGTATGG TCTCGCCTCA TTCTTCTTAT TCAACAAACA TTATTCCAAA TGCCATACTT 
TCATATTCCT CAGAAGTAAT AAGTATTCAC ACTCCCCACA GAGGTGGACT TCTGGACAAG 
AAACCAAGAG TCCACAGTTC ATCTTCTAAC AGAAATGGTA TTAGCAGGGA CTATACTTCT 
GGCATTAAAG ATAATTCCTC ACCTGGTAAC TGGCGTGAAA ACAGTTCATC TAGGAACTCA 
CAGATATCAG ATACAAAGTC ACCTATCAGT AAAAAAAGAG ATGTTCCACA CAACGTTCCT 
TGCAAAATTA ACACTGGCTT TAACAACTAC TCATTTGACA GTGACAATCA AAGTGAATTA 
AGCTACTCAG ATGGTCGTCA AAGAAATGGT AGTATAACTA GCAGCATTAA TAGTGCTGAC 
AACTGGTTGT ATGAATCTAG GCACAATGAC GGTGCTCCAT CTAGGAAACT ATCTTCTGTA 
ATTTCTGGGT CTCCTGTTAG CAATATGAGC GCCTGCAGTT CTAAGGGGAA GGTAGATTCC 
AGCTCTTTGT ATTTTCTGAA CCATGATGAT TTTTGCACAT CAATGCATAT AGATTCTGGA 
ATTAGTTTGA CGGACCAAAG CATTCAGAAT CTACACTATA ATATAAATAT CTTTGAAACA 
AAAAAAGCTG CAAATATAGA AGTGAAAACA GGATATAGTG ACAAATCACT TCCACGTAGC 
ATATCTTTAA AGAAATCTAA AAATCCTCCC CCCCCACCTT CCAGGACAGA CTCACTTAGA 
AGGCCATCTA AGAAAGATAT TCAGACCAGT GGGCAGGTGC TCAGTGAAAC TCTTATTGCT 
ACGCTTCAAC AGTCCTTGCA ACTAAACTTG AAGAGTAAGA GCACTGGGTC CACTTTGCAG 
TCAAGTCACT GTATGGATTA TGCAGATCCC TGCATCATGT GCTCATGTAG CCAGAGTACA 
CTTAGCAGCA CTAGTAGTGG AAAATCTGTT ATTGCTCCAA ATATGTACAC TCTCTGTACT 
GTGACACCCT CTCAGAGTGA AACCAGTAGC ATGAAATCTG AATATGCGGA TCAGTGGAGT 
TCCTTTATTG ACTGTTCACA GACTCCAAAA GAGCAACCCA AATCATTTGC AAAACACAAT 
CTAAGTAATC CTGATGGTCT TAATAATTAC AGTGCATGTG AGCTGACCTA TGGAATGAAG 
ACCATAGCAC CACATGGGTA CACTGGATCA GTTAAAGTTA GTAATTCCTC TTCAGAAAAA 
ACATACAGAG TTACATCACC ATCTAGTGGA TATTCAAGTC AGTCAAACAC TCCCACCACA 
TTGACACCAG TGCCTCTTTT CTCAAAGAAT ATATATCCAG AAAATGGAAA ATACAAGTTA 
AAACCAAAAG TACCTGAAAG ACGATCTTCA TTGGTATCCT CGGTTTCAGT TTCATCTTCC 
TCTACTTCCC TTTCTTCAAA TACATTGGAC TCTCTGCAGC AGAACATGCA AATTTCAACT 
GTTATGTTGT CAGCCCATCA CACTCTTCCA ACCCCACAAC TCACTCCTGA CATGCCCCCA 
CCCCCAGCAA TGAATGCAAC CAACACAATT TTTGTCGAGT GTTCACCAAA ATGTCCACCT 
CCTTCCCTTG AATTTACAAG GCTAACAGCG GATAGCATGG ATATGTGGCT TCCCTTCTCT 
CAAAACACTG CCATGAGTAA ACCTAAGGAC AGTCTTCCAT ATCCTTCCTT TCCACCTTCT 
CTTTCCATGA TGCACAGCAT GGTCCCATCA CCTGTACCTC CACTTAACAT TGAAATGCTG 
AAAATTGAAA CAGAATCATT TCCTCTCAGA AATTCTGAAA GCAGTTATTT TGTACATAAT 
GATCACAGTA AACATGAACT GTTGATACCA ACTGTTCCTA TAGTTACTTC TCATGCACTA 
GAAATGGTTC ATTTGAGATC TGTGAAAAAA GCATCACAAC TGGAAACTAA AACATTAAGA 
GAACACATGT CAGAATCCCA CCTACAGGAT GAGACATCCG CCTTATTGGT ACAGCCATGT 
TTAGTACCAG CTGCTTCTCC AGAACTAAAT TCTAGTGAAA GCCAAATAGA AAATTTCAGC 
ATTCCTGCCC AGCAAGTCTC TGGTTTTAAA AGCAATGCAG TGAAACTAAC TATTGGTGAA 
ATAGAAGCAG ACAAGGCATA TAAAGAGCAC ATAGAAAAAA ATACAAATTC AGCATACAAT 
GGAAACTCAT CAGGGCCCAA ATTATCTCCT CATGAGAAAC CAAGTATGTC ACAAAGCACA 
CCAAATTCAT CTCTTAGCAA GGAGCCGCCT CTTGTTTCAA AGAAGCCTAA ACTTGTACTT 
ATTGCCCCAA CGCCACAACT TGATTTGGCA GCAGAAAAAA TAGCTCAAGT CACTGAAAAT 
GTGAGAAGCA TGCCAAATGC CTTTGAAAAA GATTCAGTTA GCAAATGTTG TGTGGGGGAG 
ATAAATGACA GAACTAAAGA TGAAGACATA GCAGGTCCAA CCAATGGTTT GGCTCACCAA 
GAACAAGAAG CCTGGTTATC CCCATGCAAA GCTGCTAAAA ATATGATTTT CATACTACCA 
TCAGTTCCAG AAATTCAGAA TCATCAGCAA AAGGAAGAGC AGCAATCCAT GTCAGATGAA 
TTCAACATTA GTGGTAACAT TTCTGAGACT GAGAATACAA GCTGCAACCA GAATGCATCT 
CAAGATGATG AGAGTGCTGA AGTGTTTAAT ACAGAGACGG TGAATTCAGT TCTCTGGACA 
AGCAGCGACA TTATGGGAAA TGTAGAAGAG ACCACTCCAA CAAGGCCTGC TACAACTGAG 
GATCTTTTTG CTCGTATCCA TAGATCCAAA AAAAAAATTC TTGGACGCAA AGATTCTGAC 
GATGATCGTT GCAGAGATCA CTCGCCTTCT TCTCCAGTTA CCCCAACTGG TAATATGCAT 
CTAAGTTCCT TCAAGCAGAC AGGTTCAATC CAAAGAAGTG TCAGAAAAAC TACAACAAGT 
AGTGACAGTT TCAAAGCACT GCTGTTAAAA AAGGGAAGCA GGTCTGAAAG TGGCTTCCGC 
ATGTCTGCTG CAGAAATGCT GAAACATACT GATCCAAGGT TTCAGAGATC AAGATCAGAA 
TCATCTCTGG AGTTTCCAGA AAGCCCCACA TTGTCATCAC CAAACAAGAA CAAGAAGGAG 
CAACAGGAAT GGGCAAAAAG TGAAGGGCTG ATGCCTAGAA GTATGTCTGG CTCTGTTGTC 
CGGTGCAGCC GCGCCAAGAC TCCCCCTTCT GCATCTAGTA GCAAATACAA TGTCCGTAGT 
CGTCTCCAGA GTAGCCCAAT GACTGTTATA TGTGAAGGAG AAGGAGAGGG TTCAGAAACA 
GTGGATAGCA GCTGTTCAAA GGGTCCTCTT TTGTTGAGCA CAAAGAGTCT CGATATCTGT 
TACAGTGCTG ATAAAGAAGT GACCAACCAC ATACTGCACA TAAACTTTAA CACACTATTA 
GAAAAGAGCA CAGATGTTGC TAGTGGCTGC ACAGGGGAAA TAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818257.1
CDS	28..4695
Translation	MGNKPGGCRVGPATRSSQQTRSHRVCCFPSAGPMPRGTSCSRTAAPRTWQASGQNVTSFGRMAVAVHSTTPRDQHSRHSDWIAQAIRKRKIHMSQSANPPWTRGQPFSVSFNEEEQILSTKRPKTPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHRSKKKILGRKDSDDDRCRDHSPSSPVTPTGNMHLSSFKQTGSIQRSVRKTTTSSDSFKALLLKKGSRSESGFRMSAAEMLKHTDPRFQRSRSESSLEFPESPTLSSPNKNKKEQQEWAKSEGLMPRSMSGSVVRCSRAKTPPSASSSKYNVRSRLQSSPMTVICEGEGEGSETVDSSCSKGPLLLSTKSLDICYSADKEVTNHILHINFNTLLEKSTDVASGCTGEII

Target ORF information:

RefSeq Version	XM_012962803.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962803.2

ORF Insert Sequence:

ATGGGCAACA AGCCGGGGGG CTGTCGTGTC GGCCCTGCAA CGCGATCGTC GCAGCAGACG 
CGATCACATC GCGTCTGCTG CTTCCCCAGT GCCGGCCCAA TGCCTAGGGG GACTTCCTGC 
TCCAGGACTG CAGCACCGAG GACCTGGCAA GCTTCAGGAC AGAATGTGAC AAGCTTCGGA 
AGGATGGCTG TCGCAGTTCA CAGTACTACT CCCAGGGACC AACATTCTCG ACACTCAGAC 
TGGATTGCAC AAGCTATCAG GAAGAGGAAA ATACACATGA GTCAAAGTGC AAACCCACCA 
TGGACCCGCG GCCAGCCTTT TTCTGTTTCA TTTAATGAAG AGGAACAGAT CCTTTCAACC 
AAAAGGCCCA AGACTCCTGT GTTGCTTGAG TTTTCTGAAA CGCTCACTAA CTGGACCAAG 
TCACTGCCAC TGCCTACTCC AGAGGAAAAG ATGCGTCAGG AGGCTCAGGC AGTACAGACA 
GATGTAATTC CTATAAACAT AACAGGGGAG AATTTCAACC GCCAGGCCAG CTTTCAGCGG 
TCACTTATTT ATACAGACAC TGCCGTAAGA TGTTCGAAGA AAGTCAAAAG GAGAAAGACT 
ATAACAGGCG TCCCAAACAA CTTACAGAAG GAGCTAGCAG GTACTGGACA CTCTGACCTA 
AGAAGGAATT CAGTGCTCAT ACCTGATGAT TTTAATACTT TGGAACATTG TCACCACTCA 
ATGCAATTTC TGGACACCAG AGATTTTGGC TGCCAAACCA ATAAAATAAA AATAGTGCCA 
CCATCAATTA GAAGAATCAG AGCACAGAAA GGGCAAGGAA TTGGAATTCT GATGTCCACT 
TCCTCTGGCA ACATGTCTAC TCTAAGTGAC TCTGCAGCTT GTTCTTCATA TCTAAATGGT 
AACCTGCACT TCCGTAGTCT GCCCAGGATT GGTGCAAGAG AATCTCTTCA ATCCCTTAAC 
CAAAAAAATT ATTATTACAG CAACATGGAG GACCCTTTTA CAACTTTGCC TTGGCAGTTA 
AACAAACTGC AAGTGGATGA TACTAATGTT CAATTAAGGA GTAACCCTAG GTCAACTATT 
AATCAAAGGC CTGAGTCTCA AGAAGTAAAA AATTCTACTA GTGAAATTGC CTCAAGTCCA 
GCATGTATGG TCTCGCCTCA TTCTTCTTAT TCAACAAACA TTATTCCAAA TGCCATACTT 
TCATATTCCT CAGAAGTAAT AAGTATTCAC ACTCCCCACA GAGGTGGACT TCTGGACAAG 
AAACCAAGAG TCCACAGTTC ATCTTCTAAC AGAAATGGTA TTAGCAGGGA CTATACTTCT 
GGCATTAAAG ATAATTCCTC ACCTGGTAAC TGGCGTGAAA ACAGTTCATC TAGGAACTCA 
CAGATATCAG ATACAAAGTC ACCTATCAGT AAAAAAAGAG ATGTTCCACA CAACGTTCCT 
TGCAAAATTA ACACTGGCTT TAACAACTAC TCATTTGACA GTGACAATCA AAGTGAATTA 
AGCTACTCAG ATGGTCGTCA AAGAAATGGT AGTATAACTA GCAGCATTAA TAGTGCTGAC 
AACTGGTTGT ATGAATCTAG GCACAATGAC GGTGCTCCAT CTAGGAAACT ATCTTCTGTA 
ATTTCTGGGT CTCCTGTTAG CAATATGAGC GCCTGCAGTT CTAAGGGGAA GGTAGATTCC 
AGCTCTTTGT ATTTTCTGAA CCATGATGAT TTTTGCACAT CAATGCATAT AGATTCTGGA 
ATTAGTTTGA CGGACCAAAG CATTCAGAAT CTACACTATA ATATAAATAT CTTTGAAACA 
AAAAAAGCTG CAAATATAGA AGTGAAAACA GGATATAGTG ACAAATCACT TCCACGTAGC 
ATATCTTTAA AGAAATCTAA AAATCCTCCC CCCCCACCTT CCAGGACAGA CTCACTTAGA 
AGGCCATCTA AGAAAGATAT TCAGACCAGT GGGCAGGTGC TCAGTGAAAC TCTTATTGCT 
ACGCTTCAAC AGTCCTTGCA ACTAAACTTG AAGAGTAAGA GCACTGGGTC CACTTTGCAG 
TCAAGTCACT GTATGGATTA TGCAGATCCC TGCATCATGT GCTCATGTAG CCAGAGTACA 
CTTAGCAGCA CTAGTAGTGG AAAATCTGTT ATTGCTCCAA ATATGTACAC TCTCTGTACT 
GTGACACCCT CTCAGAGTGA AACCAGTAGC ATGAAATCTG AATATGCGGA TCAGTGGAGT 
TCCTTTATTG ACTGTTCACA GACTCCAAAA GAGCAACCCA AATCATTTGC AAAACACAAT 
CTAAGTAATC CTGATGGTCT TAATAATTAC AGTGCATGTG AGCTGACCTA TGGAATGAAG 
ACCATAGCAC CACATGGGTA CACTGGATCA GTTAAAGTTA GTAATTCCTC TTCAGAAAAA 
ACATACAGAG TTACATCACC ATCTAGTGGA TATTCAAGTC AGTCAAACAC TCCCACCACA 
TTGACACCAG TGCCTCTTTT CTCAAAGAAT ATATATCCAG AAAATGGAAA ATACAAGTTA 
AAACCAAAAG TACCTGAAAG ACGATCTTCA TTGGTATCCT CGGTTTCAGT TTCATCTTCC 
TCTACTTCCC TTTCTTCAAA TACATTGGAC TCTCTGCAGC AGAACATGCA AATTTCAACT 
GTTATGTTGT CAGCCCATCA CACTCTTCCA ACCCCACAAC TCACTCCTGA CATGCCCCCA 
CCCCCAGCAA TGAATGCAAC CAACACAATT TTTGTCGAGT GTTCACCAAA ATGTCCACCT 
CCTTCCCTTG AATTTACAAG GCTAACAGCG GATAGCATGG ATATGTGGCT TCCCTTCTCT 
CAAAACACTG CCATGAGTAA ACCTAAGGAC AGTCTTCCAT ATCCTTCCTT TCCACCTTCT 
CTTTCCATGA TGCACAGCAT GGTCCCATCA CCTGTACCTC CACTTAACAT TGAAATGCTG 
AAAATTGAAA CAGAATCATT TCCTCTCAGA AATTCTGAAA GCAGTTATTT TGTACATAAT 
GATCACAGTA AACATGAACT GTTGATACCA ACTGTTCCTA TAGTTACTTC TCATGCACTA 
GAAATGGTTC ATTTGAGATC TGTGAAAAAA GCATCACAAC TGGAAACTAA AACATTAAGA 
GAACACATGT CAGAATCCCA CCTACAGGAT GAGACATCCG CCTTATTGGT ACAGCCATGT 
TTAGTACCAG CTGCTTCTCC AGAACTAAAT TCTAGTGAAA GCCAAATAGA AAATTTCAGC 
ATTCCTGCCC AGCAAGTCTC TGGTTTTAAA AGCAATGCAG TGAAACTAAC TATTGGTGAA 
ATAGAAGCAG ACAAGGCATA TAAAGAGCAC ATAGAAAAAA ATACAAATTC AGCATACAAT 
GGAAACTCAT CAGGGCCCAA ATTATCTCCT CATGAGAAAC CAAGTATGTC ACAAAGCACA 
CCAAATTCAT CTCTTAGCAA GGAGCCGCCT CTTGTTTCAA AGAAGCCTAA ACTTGTACTT 
ATTGCCCCAA CGCCACAACT TGATTTGGCA GCAGAAAAAA TAGCTCAAGT CACTGAAAAT 
GTGAGAAGCA TGCCAAATGC CTTTGAAAAA GATTCAGTTA GCAAATGTTG TGTGGGGGAG 
ATAAATGACA GAACTAAAGA TGAAGACATA GCAGGTCCAA CCAATGGTTT GGCTCACCAA 
GAACAAGAAG CCTGGTTATC CCCATGCAAA GCTGCTAAAA ATATGATTTT CATACTACCA 
TCAGTTCCAG AAATTCAGAA TCATCAGCAA AAGGAAGAGC AGCAATCCAT GTCAGATGAA 
TTCAACATTA GTGGTAACAT TTCTGAGACT GAGAATACAA GCTGCAACCA GAATGCATCT 
CAAGATGATG AGAGTGCTGA AGTGTTTAAT ACAGAGACGG TGAATTCAGT TCTCTGGACA 
AGCAGCGACA TTATGGGAAA TGTAGAAGAG ACCACTCCAA CAAGGCCTGC TACAACTGAG 
GATCTTTTTG CTCGTATCCA TAGATCCAAA AAAAAAATTC TTGGACGCAA AGATTCTGAC 
GATGATCGTT GCAGAGATCA CTCGCCTTCT TCTCCAGTTA CCCCAACTGG TAATATGCAT 
CTAAGTTCCT TCAAGCAGAC AGGTTCAATC CAAAGAAGTG TCAGAAAAAC TACAACAAGT 
AGTGACAGTT TCAAAGCACT GCTGTTAAAA AAGGGAAGCA GGTCTGAAAG TGGCTTCCGC 
ATGTCTGCTG CAGAAATGCT GAAACATACT GATCCAAGGT TTCAGAGATC AAGATCAGAA 
TCATCTCTGG AGTTTCCAGA AAGCCCCACA TTGTCATCAC CAAACAAGAA CAAGAAGGAG 
CAACAGGAAT GGGCAAAAAG TGAAGGGCTG ATGCCTAGAA GTATGTCTGG CTCTGTTGTC 
CGGTGCAGCC GCGCCAAGAC TCCCCCTTCT GCATCTAGTA GCAAATACAA TGTCCGTAGT 
CGTCTCCAGA GTAGCCCAAT GACTGTTATA TGTGAAGGAG AAGGAGAGGG TTCAGAAACA 
GTGGATAGCA GCTGTTCAAA GGGTCCTCTT TTGTTGAGCA CAAAGAGTCT CGATATCTGT 
TACAGTGCTG ATAAAGAAGT GACCAACCAC ATACTGCACA TAAACTTTAA CACACTATTA 
GAAAAGAGCA CAGATGTTGC TAGTGGCTGC ACAGGGGAAA TAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_012962805.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818259.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa32639
Clone ID Related Accession (Same CDS sequence)	XM_012962805.2 , XM_012962804.2
Accession Version	XM_012962805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCG CAGTTCACAG TACTACTCCC AGGGACCAAC ATTCTCGACA CTCAGACTGG 
ATTGCACAAG CTATCAGGAA GAGGAAAATA CACATGAGTC AAAGTGCAAA CCCACCATGG 
ACCCGCGGCC AGCCTTTTTC TGTTTCATTT AATGAAGAGG AACAGATCCT TTCAACCAAA 
AGGCCCAAGA CTCCTGTGTT GCTTGAGTTT TCTGAAACGC TCACTAACTG GACCAAGTCA 
CTGCCACTGC CTACTCCAGA GGAAAAGATG CGTCAGGAGG CTCAGGCAGT ACAGACAGAT 
GTAATTCCTA TAAACATAAC AGGGGAGAAT TTCAACCGCC AGGCCAGCTT TCAGCGGTCA 
CTTATTTATA CAGACACTGC CGTAAGATGT TCGAAGAAAG TCAAAAGGAG AAAGACTATA 
ACAGGCGTCC CAAACAACTT ACAGAAGGAG CTAGCAGGTA CTGGACACTC TGACCTAAGA 
AGGAATTCAG TGCTCATACC TGATGATTTT AATACTTTGG AACATTGTCA CCACTCAATG 
CAATTTCTGG ACACCAGAGA TTTTGGCTGC CAAACCAATA AAATAAAAAT AGTGCCACCA 
TCAATTAGAA GAATCAGAGC ACAGAAAGGG CAAGGAATTG GAATTCTGAT GTCCACTTCC 
TCTGGCAACA TGTCTACTCT AAGTGACTCT GCAGCTTGTT CTTCATATCT AAATGGTAAC 
CTGCACTTCC GTAGTCTGCC CAGGATTGGT GCAAGAGAAT CTCTTCAATC CCTTAACCAA 
AAAAATTATT ATTACAGCAA CATGGAGGAC CCTTTTACAA CTTTGCCTTG GCAGTTAAAC 
AAACTGCAAG TGGATGATAC TAATGTTCAA TTAAGGAGTA ACCCTAGGTC AACTATTAAT 
CAAAGGCCTG AGTCTCAAGA AGTAAAAAAT TCTACTAGTG AAATTGCCTC AAGTCCAGCA 
TGTATGGTCT CGCCTCATTC TTCTTATTCA ACAAACATTA TTCCAAATGC CATACTTTCA 
TATTCCTCAG AAGTAATAAG TATTCACACT CCCCACAGAG GTGGACTTCT GGACAAGAAA 
CCAAGAGTCC ACAGTTCATC TTCTAACAGA AATGGTATTA GCAGGGACTA TACTTCTGGC 
ATTAAAGATA ATTCCTCACC TGGTAACTGG CGTGAAAACA GTTCATCTAG GAACTCACAG 
ATATCAGATA CAAAGTCACC TATCAGTAAA AAAAGAGATG TTCCACACAA CGTTCCTTGC 
AAAATTAACA CTGGCTTTAA CAACTACTCA TTTGACAGTG ACAATCAAAG TGAATTAAGC 
TACTCAGATG GTCGTCAAAG AAATGGTAGT ATAACTAGCA GCATTAATAG TGCTGACAAC 
TGGTTGTATG AATCTAGGCA CAATGACGGT GCTCCATCTA GGAAACTATC TTCTGTAATT 
TCTGGGTCTC CTGTTAGCAA TATGAGCGCC TGCAGTTCTA AGGGGAAGGT AGATTCCAGC 
TCTTTGTATT TTCTGAACCA TGATGATTTT TGCACATCAA TGCATATAGA TTCTGGAATT 
AGTTTGACGG ACCAAAGCAT TCAGAATCTA CACTATAATA TAAATATCTT TGAAACAAAA 
AAAGCTGCAA ATATAGAAGT GAAAACAGGA TATAGTGACA AATCACTTCC ACGTAGCATA 
TCTTTAAAGA AATCTAAAAA TCCTCCCCCC CCACCTTCCA GGACAGACTC ACTTAGAAGG 
CCATCTAAGA AAGATATTCA GACCAGTGGG CAGGTGCTCA GTGAAACTCT TATTGCTACG 
CTTCAACAGT CCTTGCAACT AAACTTGAAG AGTAAGAGCA CTGGGTCCAC TTTGCAGTCA 
AGTCACTGTA TGGATTATGC AGATCCCTGC ATCATGTGCT CATGTAGCCA GAGTACACTT 
AGCAGCACTA GTAGTGGAAA ATCTGTTATT GCTCCAAATA TGTACACTCT CTGTACTGTG 
ACACCCTCTC AGAGTGAAAC CAGTAGCATG AAATCTGAAT ATGCGGATCA GTGGAGTTCC 
TTTATTGACT GTTCACAGAC TCCAAAAGAG CAACCCAAAT CATTTGCAAA ACACAATCTA 
AGTAATCCTG ATGGTCTTAA TAATTACAGT GCATGTGAGC TGACCTATGG AATGAAGACC 
ATAGCACCAC ATGGGTACAC TGGATCAGTT AAAGTTAGTA ATTCCTCTTC AGAAAAAACA 
TACAGAGTTA CATCACCATC TAGTGGATAT TCAAGTCAGT CAAACACTCC CACCACATTG 
ACACCAGTGC CTCTTTTCTC AAAGAATATA TATCCAGAAA ATGGAAAATA CAAGTTAAAA 
CCAAAAGTAC CTGAAAGACG ATCTTCATTG GTATCCTCGG TTTCAGTTTC ATCTTCCTCT 
ACTTCCCTTT CTTCAAATAC ATTGGACTCT CTGCAGCAGA ACATGCAAAT TTCAACTGTT 
ATGTTGTCAG CCCATCACAC TCTTCCAACC CCACAACTCA CTCCTGACAT GCCCCCACCC 
CCAGCAATGA ATGCAACCAA CACAATTTTT GTCGAGTGTT CACCAAAATG TCCACCTCCT 
TCCCTTGAAT TTACAAGGCT AACAGCGGAT AGCATGGATA TGTGGCTTCC CTTCTCTCAA 
AACACTGCCA TGAGTAAACC TAAGGACAGT CTTCCATATC CTTCCTTTCC ACCTTCTCTT 
TCCATGATGC ACAGCATGGT CCCATCACCT GTACCTCCAC TTAACATTGA AATGCTGAAA 
ATTGAAACAG AATCATTTCC TCTCAGAAAT TCTGAAAGCA GTTATTTTGT ACATAATGAT 
CACAGTAAAC ATGAACTGTT GATACCAACT GTTCCTATAG TTACTTCTCA TGCACTAGAA 
ATGGTTCATT TGAGATCTGT GAAAAAAGCA TCACAACTGG AAACTAAAAC ATTAAGAGAA 
CACATGTCAG AATCCCACCT ACAGGATGAG ACATCCGCCT TATTGGTACA GCCATGTTTA 
GTACCAGCTG CTTCTCCAGA ACTAAATTCT AGTGAAAGCC AAATAGAAAA TTTCAGCATT 
CCTGCCCAGC AAGTCTCTGG TTTTAAAAGC AATGCAGTGA AACTAACTAT TGGTGAAATA 
GAAGCAGACA AGGCATATAA AGAGCACATA GAAAAAAATA CAAATTCAGC ATACAATGGA 
AACTCATCAG GGCCCAAATT ATCTCCTCAT GAGAAACCAA GTATGTCACA AAGCACACCA 
AATTCATCTC TTAGCAAGGA GCCGCCTCTT GTTTCAAAGA AGCCTAAACT TGTACTTATT 
GCCCCAACGC CACAACTTGA TTTGGCAGCA GAAAAAATAG CTCAAGTCAC TGAAAATGTG 
AGAAGCATGC CAAATGCCTT TGAAAAAGAT TCAGTTAGCA AATGTTGTGT GGGGGAGATA 
AATGACAGAA CTAAAGATGA AGACATAGCA GGTCCAACCA ATGGTTTGGC TCACCAAGAA 
CAAGAAGCCT GGTTATCCCC ATGCAAAGCT GCTAAAAATA TGATTTTCAT ACTACCATCA 
GTTCCAGAAA TTCAGAATCA TCAGCAAAAG GAAGAGCAGC AATCCATGTC AGATGAATTC 
AACATTAGTG GTAACATTTC TGAGACTGAG AATACAAGCT GCAACCAGAA TGCATCTCAA 
GATGATGAGA GTGCTGAAGT GTTTAATACA GAGACGGTGA ATTCAGTTCT CTGGACAAGC 
AGCGACATTA TGGGAAATGT AGAAGAGACC ACTCCAACAA GGCCTGCTAC AACTGAGGAT 
CTTTTTGCTC GTATCCATAG ATCCAAAAAA AAAATTCTTG GACGCAAAGA TTCTGACGAT 
GATCGTTGCA GAGATCACTC GCCTTCTTCT CCAGTTACCC CAACTGGTAA TATGCATCTA 
AGTTCCTTCA AGCAGACAGG TTCAATCCAA AGAAGTGTCA GAAAAACTAC AACAAGTAGT 
GACAGTTTCA AAGCACTGCT GTTAAAAAAG GGAAGCAGGT CTGAAAGTGG CTTCCGCATG 
TCTGCTGCAG AAATGCTGAA ACATACTGAT CCAAGGTTTC AGAGATCAAG ATCAGAATCA 
TCTCTGGAGT TTCCAGAAAG CCCCACATTG TCATCACCAA ACAAGAACAA GAAGGAGCAA 
CAGGAATGGG CAAAAAGTGA AGGGCTGATG CCTAGAAGTA TGTCTGGCTC TGTTGTCCGG 
TGCAGCCGCG CCAAGACTCC CCCTTCTGCA TCTAGTAGCA AATACAATGT CCGTAGTCGT 
CTCCAGAGTA GCCCAATGAC TGTTATATGT GAAGGAGAAG GAGAGGGTTC AGAAACAGTG 
GATAGCAGCT GTTCAAAGGG TCCTCTTTTG TTGAGCACAA AGAGTCTCGA TATCTGTTAC 
AGTGCTGATA AAGAAGTGAC CAACCACATA CTGCACATAA ACTTTAACAC ACTATTAGAA 
AAGAGCACAG ATGTTGCTAG TGGCTGCACA GGGGAAATAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818259.1
CDS	94..4578
Translation	MAVAVHSTTPRDQHSRHSDWIAQAIRKRKIHMSQSANPPWTRGQPFSVSFNEEEQILSTKRPKTPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHRSKKKILGRKDSDDDRCRDHSPSSPVTPTGNMHLSSFKQTGSIQRSVRKTTTSSDSFKALLLKKGSRSESGFRMSAAEMLKHTDPRFQRSRSESSLEFPESPTLSSPNKNKKEQQEWAKSEGLMPRSMSGSVVRCSRAKTPPSASSSKYNVRSRLQSSPMTVICEGEGEGSETVDSSCSKGPLLLSTKSLDICYSADKEVTNHILHINFNTLLEKSTDVASGCTGEII

Target ORF information:

RefSeq Version	XM_012962805.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962805.2

ORF Insert Sequence:

ATGGCTGTCG CAGTTCACAG TACTACTCCC AGGGACCAAC ATTCTCGACA CTCAGACTGG 
ATTGCACAAG CTATCAGGAA GAGGAAAATA CACATGAGTC AAAGTGCAAA CCCACCATGG 
ACCCGCGGCC AGCCTTTTTC TGTTTCATTT AATGAAGAGG AACAGATCCT TTCAACCAAA 
AGGCCCAAGA CTCCTGTGTT GCTTGAGTTT TCTGAAACGC TCACTAACTG GACCAAGTCA 
CTGCCACTGC CTACTCCAGA GGAAAAGATG CGTCAGGAGG CTCAGGCAGT ACAGACAGAT 
GTAATTCCTA TAAACATAAC AGGGGAGAAT TTCAACCGCC AGGCCAGCTT TCAGCGGTCA 
CTTATTTATA CAGACACTGC CGTAAGATGT TCGAAGAAAG TCAAAAGGAG AAAGACTATA 
ACAGGCGTCC CAAACAACTT ACAGAAGGAG CTAGCAGGTA CTGGACACTC TGACCTAAGA 
AGGAATTCAG TGCTCATACC TGATGATTTT AATACTTTGG AACATTGTCA CCACTCAATG 
CAATTTCTGG ACACCAGAGA TTTTGGCTGC CAAACCAATA AAATAAAAAT AGTGCCACCA 
TCAATTAGAA GAATCAGAGC ACAGAAAGGG CAAGGAATTG GAATTCTGAT GTCCACTTCC 
TCTGGCAACA TGTCTACTCT AAGTGACTCT GCAGCTTGTT CTTCATATCT AAATGGTAAC 
CTGCACTTCC GTAGTCTGCC CAGGATTGGT GCAAGAGAAT CTCTTCAATC CCTTAACCAA 
AAAAATTATT ATTACAGCAA CATGGAGGAC CCTTTTACAA CTTTGCCTTG GCAGTTAAAC 
AAACTGCAAG TGGATGATAC TAATGTTCAA TTAAGGAGTA ACCCTAGGTC AACTATTAAT 
CAAAGGCCTG AGTCTCAAGA AGTAAAAAAT TCTACTAGTG AAATTGCCTC AAGTCCAGCA 
TGTATGGTCT CGCCTCATTC TTCTTATTCA ACAAACATTA TTCCAAATGC CATACTTTCA 
TATTCCTCAG AAGTAATAAG TATTCACACT CCCCACAGAG GTGGACTTCT GGACAAGAAA 
CCAAGAGTCC ACAGTTCATC TTCTAACAGA AATGGTATTA GCAGGGACTA TACTTCTGGC 
ATTAAAGATA ATTCCTCACC TGGTAACTGG CGTGAAAACA GTTCATCTAG GAACTCACAG 
ATATCAGATA CAAAGTCACC TATCAGTAAA AAAAGAGATG TTCCACACAA CGTTCCTTGC 
AAAATTAACA CTGGCTTTAA CAACTACTCA TTTGACAGTG ACAATCAAAG TGAATTAAGC 
TACTCAGATG GTCGTCAAAG AAATGGTAGT ATAACTAGCA GCATTAATAG TGCTGACAAC 
TGGTTGTATG AATCTAGGCA CAATGACGGT GCTCCATCTA GGAAACTATC TTCTGTAATT 
TCTGGGTCTC CTGTTAGCAA TATGAGCGCC TGCAGTTCTA AGGGGAAGGT AGATTCCAGC 
TCTTTGTATT TTCTGAACCA TGATGATTTT TGCACATCAA TGCATATAGA TTCTGGAATT 
AGTTTGACGG ACCAAAGCAT TCAGAATCTA CACTATAATA TAAATATCTT TGAAACAAAA 
AAAGCTGCAA ATATAGAAGT GAAAACAGGA TATAGTGACA AATCACTTCC ACGTAGCATA 
TCTTTAAAGA AATCTAAAAA TCCTCCCCCC CCACCTTCCA GGACAGACTC ACTTAGAAGG 
CCATCTAAGA AAGATATTCA GACCAGTGGG CAGGTGCTCA GTGAAACTCT TATTGCTACG 
CTTCAACAGT CCTTGCAACT AAACTTGAAG AGTAAGAGCA CTGGGTCCAC TTTGCAGTCA 
AGTCACTGTA TGGATTATGC AGATCCCTGC ATCATGTGCT CATGTAGCCA GAGTACACTT 
AGCAGCACTA GTAGTGGAAA ATCTGTTATT GCTCCAAATA TGTACACTCT CTGTACTGTG 
ACACCCTCTC AGAGTGAAAC CAGTAGCATG AAATCTGAAT ATGCGGATCA GTGGAGTTCC 
TTTATTGACT GTTCACAGAC TCCAAAAGAG CAACCCAAAT CATTTGCAAA ACACAATCTA 
AGTAATCCTG ATGGTCTTAA TAATTACAGT GCATGTGAGC TGACCTATGG AATGAAGACC 
ATAGCACCAC ATGGGTACAC TGGATCAGTT AAAGTTAGTA ATTCCTCTTC AGAAAAAACA 
TACAGAGTTA CATCACCATC TAGTGGATAT TCAAGTCAGT CAAACACTCC CACCACATTG 
ACACCAGTGC CTCTTTTCTC AAAGAATATA TATCCAGAAA ATGGAAAATA CAAGTTAAAA 
CCAAAAGTAC CTGAAAGACG ATCTTCATTG GTATCCTCGG TTTCAGTTTC ATCTTCCTCT 
ACTTCCCTTT CTTCAAATAC ATTGGACTCT CTGCAGCAGA ACATGCAAAT TTCAACTGTT 
ATGTTGTCAG CCCATCACAC TCTTCCAACC CCACAACTCA CTCCTGACAT GCCCCCACCC 
CCAGCAATGA ATGCAACCAA CACAATTTTT GTCGAGTGTT CACCAAAATG TCCACCTCCT 
TCCCTTGAAT TTACAAGGCT AACAGCGGAT AGCATGGATA TGTGGCTTCC CTTCTCTCAA 
AACACTGCCA TGAGTAAACC TAAGGACAGT CTTCCATATC CTTCCTTTCC ACCTTCTCTT 
TCCATGATGC ACAGCATGGT CCCATCACCT GTACCTCCAC TTAACATTGA AATGCTGAAA 
ATTGAAACAG AATCATTTCC TCTCAGAAAT TCTGAAAGCA GTTATTTTGT ACATAATGAT 
CACAGTAAAC ATGAACTGTT GATACCAACT GTTCCTATAG TTACTTCTCA TGCACTAGAA 
ATGGTTCATT TGAGATCTGT GAAAAAAGCA TCACAACTGG AAACTAAAAC ATTAAGAGAA 
CACATGTCAG AATCCCACCT ACAGGATGAG ACATCCGCCT TATTGGTACA GCCATGTTTA 
GTACCAGCTG CTTCTCCAGA ACTAAATTCT AGTGAAAGCC AAATAGAAAA TTTCAGCATT 
CCTGCCCAGC AAGTCTCTGG TTTTAAAAGC AATGCAGTGA AACTAACTAT TGGTGAAATA 
GAAGCAGACA AGGCATATAA AGAGCACATA GAAAAAAATA CAAATTCAGC ATACAATGGA 
AACTCATCAG GGCCCAAATT ATCTCCTCAT GAGAAACCAA GTATGTCACA AAGCACACCA 
AATTCATCTC TTAGCAAGGA GCCGCCTCTT GTTTCAAAGA AGCCTAAACT TGTACTTATT 
GCCCCAACGC CACAACTTGA TTTGGCAGCA GAAAAAATAG CTCAAGTCAC TGAAAATGTG 
AGAAGCATGC CAAATGCCTT TGAAAAAGAT TCAGTTAGCA AATGTTGTGT GGGGGAGATA 
AATGACAGAA CTAAAGATGA AGACATAGCA GGTCCAACCA ATGGTTTGGC TCACCAAGAA 
CAAGAAGCCT GGTTATCCCC ATGCAAAGCT GCTAAAAATA TGATTTTCAT ACTACCATCA 
GTTCCAGAAA TTCAGAATCA TCAGCAAAAG GAAGAGCAGC AATCCATGTC AGATGAATTC 
AACATTAGTG GTAACATTTC TGAGACTGAG AATACAAGCT GCAACCAGAA TGCATCTCAA 
GATGATGAGA GTGCTGAAGT GTTTAATACA GAGACGGTGA ATTCAGTTCT CTGGACAAGC 
AGCGACATTA TGGGAAATGT AGAAGAGACC ACTCCAACAA GGCCTGCTAC AACTGAGGAT 
CTTTTTGCTC GTATCCATAG ATCCAAAAAA AAAATTCTTG GACGCAAAGA TTCTGACGAT 
GATCGTTGCA GAGATCACTC GCCTTCTTCT CCAGTTACCC CAACTGGTAA TATGCATCTA 
AGTTCCTTCA AGCAGACAGG TTCAATCCAA AGAAGTGTCA GAAAAACTAC AACAAGTAGT 
GACAGTTTCA AAGCACTGCT GTTAAAAAAG GGAAGCAGGT CTGAAAGTGG CTTCCGCATG 
TCTGCTGCAG AAATGCTGAA ACATACTGAT CCAAGGTTTC AGAGATCAAG ATCAGAATCA 
TCTCTGGAGT TTCCAGAAAG CCCCACATTG TCATCACCAA ACAAGAACAA GAAGGAGCAA 
CAGGAATGGG CAAAAAGTGA AGGGCTGATG CCTAGAAGTA TGTCTGGCTC TGTTGTCCGG 
TGCAGCCGCG CCAAGACTCC CCCTTCTGCA TCTAGTAGCA AATACAATGT CCGTAGTCGT 
CTCCAGAGTA GCCCAATGAC TGTTATATGT GAAGGAGAAG GAGAGGGTTC AGAAACAGTG 
GATAGCAGCT GTTCAAAGGG TCCTCTTTTG TTGAGCACAA AGAGTCTCGA TATCTGTTAC 
AGTGCTGATA AAGAAGTGAC CAACCACATA CTGCACATAA ACTTTAACAC ACTATTAGAA 
AAGAGCACAG ATGTTGCTAG TGGCTGCACA GGGGAAATAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_012962804.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818258.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa32639
Clone ID Related Accession (Same CDS sequence)	XM_012962805.2 , XM_012962804.2
Accession Version	XM_012962804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962804.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCG CAGTTCACAG TACTACTCCC AGGGACCAAC ATTCTCGACA CTCAGACTGG 
ATTGCACAAG CTATCAGGAA GAGGAAAATA CACATGAGTC AAAGTGCAAA CCCACCATGG 
ACCCGCGGCC AGCCTTTTTC TGTTTCATTT AATGAAGAGG AACAGATCCT TTCAACCAAA 
AGGCCCAAGA CTCCTGTGTT GCTTGAGTTT TCTGAAACGC TCACTAACTG GACCAAGTCA 
CTGCCACTGC CTACTCCAGA GGAAAAGATG CGTCAGGAGG CTCAGGCAGT ACAGACAGAT 
GTAATTCCTA TAAACATAAC AGGGGAGAAT TTCAACCGCC AGGCCAGCTT TCAGCGGTCA 
CTTATTTATA CAGACACTGC CGTAAGATGT TCGAAGAAAG TCAAAAGGAG AAAGACTATA 
ACAGGCGTCC CAAACAACTT ACAGAAGGAG CTAGCAGGTA CTGGACACTC TGACCTAAGA 
AGGAATTCAG TGCTCATACC TGATGATTTT AATACTTTGG AACATTGTCA CCACTCAATG 
CAATTTCTGG ACACCAGAGA TTTTGGCTGC CAAACCAATA AAATAAAAAT AGTGCCACCA 
TCAATTAGAA GAATCAGAGC ACAGAAAGGG CAAGGAATTG GAATTCTGAT GTCCACTTCC 
TCTGGCAACA TGTCTACTCT AAGTGACTCT GCAGCTTGTT CTTCATATCT AAATGGTAAC 
CTGCACTTCC GTAGTCTGCC CAGGATTGGT GCAAGAGAAT CTCTTCAATC CCTTAACCAA 
AAAAATTATT ATTACAGCAA CATGGAGGAC CCTTTTACAA CTTTGCCTTG GCAGTTAAAC 
AAACTGCAAG TGGATGATAC TAATGTTCAA TTAAGGAGTA ACCCTAGGTC AACTATTAAT 
CAAAGGCCTG AGTCTCAAGA AGTAAAAAAT TCTACTAGTG AAATTGCCTC AAGTCCAGCA 
TGTATGGTCT CGCCTCATTC TTCTTATTCA ACAAACATTA TTCCAAATGC CATACTTTCA 
TATTCCTCAG AAGTAATAAG TATTCACACT CCCCACAGAG GTGGACTTCT GGACAAGAAA 
CCAAGAGTCC ACAGTTCATC TTCTAACAGA AATGGTATTA GCAGGGACTA TACTTCTGGC 
ATTAAAGATA ATTCCTCACC TGGTAACTGG CGTGAAAACA GTTCATCTAG GAACTCACAG 
ATATCAGATA CAAAGTCACC TATCAGTAAA AAAAGAGATG TTCCACACAA CGTTCCTTGC 
AAAATTAACA CTGGCTTTAA CAACTACTCA TTTGACAGTG ACAATCAAAG TGAATTAAGC 
TACTCAGATG GTCGTCAAAG AAATGGTAGT ATAACTAGCA GCATTAATAG TGCTGACAAC 
TGGTTGTATG AATCTAGGCA CAATGACGGT GCTCCATCTA GGAAACTATC TTCTGTAATT 
TCTGGGTCTC CTGTTAGCAA TATGAGCGCC TGCAGTTCTA AGGGGAAGGT AGATTCCAGC 
TCTTTGTATT TTCTGAACCA TGATGATTTT TGCACATCAA TGCATATAGA TTCTGGAATT 
AGTTTGACGG ACCAAAGCAT TCAGAATCTA CACTATAATA TAAATATCTT TGAAACAAAA 
AAAGCTGCAA ATATAGAAGT GAAAACAGGA TATAGTGACA AATCACTTCC ACGTAGCATA 
TCTTTAAAGA AATCTAAAAA TCCTCCCCCC CCACCTTCCA GGACAGACTC ACTTAGAAGG 
CCATCTAAGA AAGATATTCA GACCAGTGGG CAGGTGCTCA GTGAAACTCT TATTGCTACG 
CTTCAACAGT CCTTGCAACT AAACTTGAAG AGTAAGAGCA CTGGGTCCAC TTTGCAGTCA 
AGTCACTGTA TGGATTATGC AGATCCCTGC ATCATGTGCT CATGTAGCCA GAGTACACTT 
AGCAGCACTA GTAGTGGAAA ATCTGTTATT GCTCCAAATA TGTACACTCT CTGTACTGTG 
ACACCCTCTC AGAGTGAAAC CAGTAGCATG AAATCTGAAT ATGCGGATCA GTGGAGTTCC 
TTTATTGACT GTTCACAGAC TCCAAAAGAG CAACCCAAAT CATTTGCAAA ACACAATCTA 
AGTAATCCTG ATGGTCTTAA TAATTACAGT GCATGTGAGC TGACCTATGG AATGAAGACC 
ATAGCACCAC ATGGGTACAC TGGATCAGTT AAAGTTAGTA ATTCCTCTTC AGAAAAAACA 
TACAGAGTTA CATCACCATC TAGTGGATAT TCAAGTCAGT CAAACACTCC CACCACATTG 
ACACCAGTGC CTCTTTTCTC AAAGAATATA TATCCAGAAA ATGGAAAATA CAAGTTAAAA 
CCAAAAGTAC CTGAAAGACG ATCTTCATTG GTATCCTCGG TTTCAGTTTC ATCTTCCTCT 
ACTTCCCTTT CTTCAAATAC ATTGGACTCT CTGCAGCAGA ACATGCAAAT TTCAACTGTT 
ATGTTGTCAG CCCATCACAC TCTTCCAACC CCACAACTCA CTCCTGACAT GCCCCCACCC 
CCAGCAATGA ATGCAACCAA CACAATTTTT GTCGAGTGTT CACCAAAATG TCCACCTCCT 
TCCCTTGAAT TTACAAGGCT AACAGCGGAT AGCATGGATA TGTGGCTTCC CTTCTCTCAA 
AACACTGCCA TGAGTAAACC TAAGGACAGT CTTCCATATC CTTCCTTTCC ACCTTCTCTT 
TCCATGATGC ACAGCATGGT CCCATCACCT GTACCTCCAC TTAACATTGA AATGCTGAAA 
ATTGAAACAG AATCATTTCC TCTCAGAAAT TCTGAAAGCA GTTATTTTGT ACATAATGAT 
CACAGTAAAC ATGAACTGTT GATACCAACT GTTCCTATAG TTACTTCTCA TGCACTAGAA 
ATGGTTCATT TGAGATCTGT GAAAAAAGCA TCACAACTGG AAACTAAAAC ATTAAGAGAA 
CACATGTCAG AATCCCACCT ACAGGATGAG ACATCCGCCT TATTGGTACA GCCATGTTTA 
GTACCAGCTG CTTCTCCAGA ACTAAATTCT AGTGAAAGCC AAATAGAAAA TTTCAGCATT 
CCTGCCCAGC AAGTCTCTGG TTTTAAAAGC AATGCAGTGA AACTAACTAT TGGTGAAATA 
GAAGCAGACA AGGCATATAA AGAGCACATA GAAAAAAATA CAAATTCAGC ATACAATGGA 
AACTCATCAG GGCCCAAATT ATCTCCTCAT GAGAAACCAA GTATGTCACA AAGCACACCA 
AATTCATCTC TTAGCAAGGA GCCGCCTCTT GTTTCAAAGA AGCCTAAACT TGTACTTATT 
GCCCCAACGC CACAACTTGA TTTGGCAGCA GAAAAAATAG CTCAAGTCAC TGAAAATGTG 
AGAAGCATGC CAAATGCCTT TGAAAAAGAT TCAGTTAGCA AATGTTGTGT GGGGGAGATA 
AATGACAGAA CTAAAGATGA AGACATAGCA GGTCCAACCA ATGGTTTGGC TCACCAAGAA 
CAAGAAGCCT GGTTATCCCC ATGCAAAGCT GCTAAAAATA TGATTTTCAT ACTACCATCA 
GTTCCAGAAA TTCAGAATCA TCAGCAAAAG GAAGAGCAGC AATCCATGTC AGATGAATTC 
AACATTAGTG GTAACATTTC TGAGACTGAG AATACAAGCT GCAACCAGAA TGCATCTCAA 
GATGATGAGA GTGCTGAAGT GTTTAATACA GAGACGGTGA ATTCAGTTCT CTGGACAAGC 
AGCGACATTA TGGGAAATGT AGAAGAGACC ACTCCAACAA GGCCTGCTAC AACTGAGGAT 
CTTTTTGCTC GTATCCATAG ATCCAAAAAA AAAATTCTTG GACGCAAAGA TTCTGACGAT 
GATCGTTGCA GAGATCACTC GCCTTCTTCT CCAGTTACCC CAACTGGTAA TATGCATCTA 
AGTTCCTTCA AGCAGACAGG TTCAATCCAA AGAAGTGTCA GAAAAACTAC AACAAGTAGT 
GACAGTTTCA AAGCACTGCT GTTAAAAAAG GGAAGCAGGT CTGAAAGTGG CTTCCGCATG 
TCTGCTGCAG AAATGCTGAA ACATACTGAT CCAAGGTTTC AGAGATCAAG ATCAGAATCA 
TCTCTGGAGT TTCCAGAAAG CCCCACATTG TCATCACCAA ACAAGAACAA GAAGGAGCAA 
CAGGAATGGG CAAAAAGTGA AGGGCTGATG CCTAGAAGTA TGTCTGGCTC TGTTGTCCGG 
TGCAGCCGCG CCAAGACTCC CCCTTCTGCA TCTAGTAGCA AATACAATGT CCGTAGTCGT 
CTCCAGAGTA GCCCAATGAC TGTTATATGT GAAGGAGAAG GAGAGGGTTC AGAAACAGTG 
GATAGCAGCT GTTCAAAGGG TCCTCTTTTG TTGAGCACAA AGAGTCTCGA TATCTGTTAC 
AGTGCTGATA AAGAAGTGAC CAACCACATA CTGCACATAA ACTTTAACAC ACTATTAGAA 
AAGAGCACAG ATGTTGCTAG TGGCTGCACA GGGGAAATAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818258.1
CDS	749..5233
Translation	MAVAVHSTTPRDQHSRHSDWIAQAIRKRKIHMSQSANPPWTRGQPFSVSFNEEEQILSTKRPKTPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHRSKKKILGRKDSDDDRCRDHSPSSPVTPTGNMHLSSFKQTGSIQRSVRKTTTSSDSFKALLLKKGSRSESGFRMSAAEMLKHTDPRFQRSRSESSLEFPESPTLSSPNKNKKEQQEWAKSEGLMPRSMSGSVVRCSRAKTPPSASSSKYNVRSRLQSSPMTVICEGEGEGSETVDSSCSKGPLLLSTKSLDICYSADKEVTNHILHINFNTLLEKSTDVASGCTGEII

Target ORF information:

RefSeq Version	XM_012962804.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962804.2

ORF Insert Sequence:

ATGGCTGTCG CAGTTCACAG TACTACTCCC AGGGACCAAC ATTCTCGACA CTCAGACTGG 
ATTGCACAAG CTATCAGGAA GAGGAAAATA CACATGAGTC AAAGTGCAAA CCCACCATGG 
ACCCGCGGCC AGCCTTTTTC TGTTTCATTT AATGAAGAGG AACAGATCCT TTCAACCAAA 
AGGCCCAAGA CTCCTGTGTT GCTTGAGTTT TCTGAAACGC TCACTAACTG GACCAAGTCA 
CTGCCACTGC CTACTCCAGA GGAAAAGATG CGTCAGGAGG CTCAGGCAGT ACAGACAGAT 
GTAATTCCTA TAAACATAAC AGGGGAGAAT TTCAACCGCC AGGCCAGCTT TCAGCGGTCA 
CTTATTTATA CAGACACTGC CGTAAGATGT TCGAAGAAAG TCAAAAGGAG AAAGACTATA 
ACAGGCGTCC CAAACAACTT ACAGAAGGAG CTAGCAGGTA CTGGACACTC TGACCTAAGA 
AGGAATTCAG TGCTCATACC TGATGATTTT AATACTTTGG AACATTGTCA CCACTCAATG 
CAATTTCTGG ACACCAGAGA TTTTGGCTGC CAAACCAATA AAATAAAAAT AGTGCCACCA 
TCAATTAGAA GAATCAGAGC ACAGAAAGGG CAAGGAATTG GAATTCTGAT GTCCACTTCC 
TCTGGCAACA TGTCTACTCT AAGTGACTCT GCAGCTTGTT CTTCATATCT AAATGGTAAC 
CTGCACTTCC GTAGTCTGCC CAGGATTGGT GCAAGAGAAT CTCTTCAATC CCTTAACCAA 
AAAAATTATT ATTACAGCAA CATGGAGGAC CCTTTTACAA CTTTGCCTTG GCAGTTAAAC 
AAACTGCAAG TGGATGATAC TAATGTTCAA TTAAGGAGTA ACCCTAGGTC AACTATTAAT 
CAAAGGCCTG AGTCTCAAGA AGTAAAAAAT TCTACTAGTG AAATTGCCTC AAGTCCAGCA 
TGTATGGTCT CGCCTCATTC TTCTTATTCA ACAAACATTA TTCCAAATGC CATACTTTCA 
TATTCCTCAG AAGTAATAAG TATTCACACT CCCCACAGAG GTGGACTTCT GGACAAGAAA 
CCAAGAGTCC ACAGTTCATC TTCTAACAGA AATGGTATTA GCAGGGACTA TACTTCTGGC 
ATTAAAGATA ATTCCTCACC TGGTAACTGG CGTGAAAACA GTTCATCTAG GAACTCACAG 
ATATCAGATA CAAAGTCACC TATCAGTAAA AAAAGAGATG TTCCACACAA CGTTCCTTGC 
AAAATTAACA CTGGCTTTAA CAACTACTCA TTTGACAGTG ACAATCAAAG TGAATTAAGC 
TACTCAGATG GTCGTCAAAG AAATGGTAGT ATAACTAGCA GCATTAATAG TGCTGACAAC 
TGGTTGTATG AATCTAGGCA CAATGACGGT GCTCCATCTA GGAAACTATC TTCTGTAATT 
TCTGGGTCTC CTGTTAGCAA TATGAGCGCC TGCAGTTCTA AGGGGAAGGT AGATTCCAGC 
TCTTTGTATT TTCTGAACCA TGATGATTTT TGCACATCAA TGCATATAGA TTCTGGAATT 
AGTTTGACGG ACCAAAGCAT TCAGAATCTA CACTATAATA TAAATATCTT TGAAACAAAA 
AAAGCTGCAA ATATAGAAGT GAAAACAGGA TATAGTGACA AATCACTTCC ACGTAGCATA 
TCTTTAAAGA AATCTAAAAA TCCTCCCCCC CCACCTTCCA GGACAGACTC ACTTAGAAGG 
CCATCTAAGA AAGATATTCA GACCAGTGGG CAGGTGCTCA GTGAAACTCT TATTGCTACG 
CTTCAACAGT CCTTGCAACT AAACTTGAAG AGTAAGAGCA CTGGGTCCAC TTTGCAGTCA 
AGTCACTGTA TGGATTATGC AGATCCCTGC ATCATGTGCT CATGTAGCCA GAGTACACTT 
AGCAGCACTA GTAGTGGAAA ATCTGTTATT GCTCCAAATA TGTACACTCT CTGTACTGTG 
ACACCCTCTC AGAGTGAAAC CAGTAGCATG AAATCTGAAT ATGCGGATCA GTGGAGTTCC 
TTTATTGACT GTTCACAGAC TCCAAAAGAG CAACCCAAAT CATTTGCAAA ACACAATCTA 
AGTAATCCTG ATGGTCTTAA TAATTACAGT GCATGTGAGC TGACCTATGG AATGAAGACC 
ATAGCACCAC ATGGGTACAC TGGATCAGTT AAAGTTAGTA ATTCCTCTTC AGAAAAAACA 
TACAGAGTTA CATCACCATC TAGTGGATAT TCAAGTCAGT CAAACACTCC CACCACATTG 
ACACCAGTGC CTCTTTTCTC AAAGAATATA TATCCAGAAA ATGGAAAATA CAAGTTAAAA 
CCAAAAGTAC CTGAAAGACG ATCTTCATTG GTATCCTCGG TTTCAGTTTC ATCTTCCTCT 
ACTTCCCTTT CTTCAAATAC ATTGGACTCT CTGCAGCAGA ACATGCAAAT TTCAACTGTT 
ATGTTGTCAG CCCATCACAC TCTTCCAACC CCACAACTCA CTCCTGACAT GCCCCCACCC 
CCAGCAATGA ATGCAACCAA CACAATTTTT GTCGAGTGTT CACCAAAATG TCCACCTCCT 
TCCCTTGAAT TTACAAGGCT AACAGCGGAT AGCATGGATA TGTGGCTTCC CTTCTCTCAA 
AACACTGCCA TGAGTAAACC TAAGGACAGT CTTCCATATC CTTCCTTTCC ACCTTCTCTT 
TCCATGATGC ACAGCATGGT CCCATCACCT GTACCTCCAC TTAACATTGA AATGCTGAAA 
ATTGAAACAG AATCATTTCC TCTCAGAAAT TCTGAAAGCA GTTATTTTGT ACATAATGAT 
CACAGTAAAC ATGAACTGTT GATACCAACT GTTCCTATAG TTACTTCTCA TGCACTAGAA 
ATGGTTCATT TGAGATCTGT GAAAAAAGCA TCACAACTGG AAACTAAAAC ATTAAGAGAA 
CACATGTCAG AATCCCACCT ACAGGATGAG ACATCCGCCT TATTGGTACA GCCATGTTTA 
GTACCAGCTG CTTCTCCAGA ACTAAATTCT AGTGAAAGCC AAATAGAAAA TTTCAGCATT 
CCTGCCCAGC AAGTCTCTGG TTTTAAAAGC AATGCAGTGA AACTAACTAT TGGTGAAATA 
GAAGCAGACA AGGCATATAA AGAGCACATA GAAAAAAATA CAAATTCAGC ATACAATGGA 
AACTCATCAG GGCCCAAATT ATCTCCTCAT GAGAAACCAA GTATGTCACA AAGCACACCA 
AATTCATCTC TTAGCAAGGA GCCGCCTCTT GTTTCAAAGA AGCCTAAACT TGTACTTATT 
GCCCCAACGC CACAACTTGA TTTGGCAGCA GAAAAAATAG CTCAAGTCAC TGAAAATGTG 
AGAAGCATGC CAAATGCCTT TGAAAAAGAT TCAGTTAGCA AATGTTGTGT GGGGGAGATA 
AATGACAGAA CTAAAGATGA AGACATAGCA GGTCCAACCA ATGGTTTGGC TCACCAAGAA 
CAAGAAGCCT GGTTATCCCC ATGCAAAGCT GCTAAAAATA TGATTTTCAT ACTACCATCA 
GTTCCAGAAA TTCAGAATCA TCAGCAAAAG GAAGAGCAGC AATCCATGTC AGATGAATTC 
AACATTAGTG GTAACATTTC TGAGACTGAG AATACAAGCT GCAACCAGAA TGCATCTCAA 
GATGATGAGA GTGCTGAAGT GTTTAATACA GAGACGGTGA ATTCAGTTCT CTGGACAAGC 
AGCGACATTA TGGGAAATGT AGAAGAGACC ACTCCAACAA GGCCTGCTAC AACTGAGGAT 
CTTTTTGCTC GTATCCATAG ATCCAAAAAA AAAATTCTTG GACGCAAAGA TTCTGACGAT 
GATCGTTGCA GAGATCACTC GCCTTCTTCT CCAGTTACCC CAACTGGTAA TATGCATCTA 
AGTTCCTTCA AGCAGACAGG TTCAATCCAA AGAAGTGTCA GAAAAACTAC AACAAGTAGT 
GACAGTTTCA AAGCACTGCT GTTAAAAAAG GGAAGCAGGT CTGAAAGTGG CTTCCGCATG 
TCTGCTGCAG AAATGCTGAA ACATACTGAT CCAAGGTTTC AGAGATCAAG ATCAGAATCA 
TCTCTGGAGT TTCCAGAAAG CCCCACATTG TCATCACCAA ACAAGAACAA GAAGGAGCAA 
CAGGAATGGG CAAAAAGTGA AGGGCTGATG CCTAGAAGTA TGTCTGGCTC TGTTGTCCGG 
TGCAGCCGCG CCAAGACTCC CCCTTCTGCA TCTAGTAGCA AATACAATGT CCGTAGTCGT 
CTCCAGAGTA GCCCAATGAC TGTTATATGT GAAGGAGAAG GAGAGGGTTC AGAAACAGTG 
GATAGCAGCT GTTCAAAGGG TCCTCTTTTG TTGAGCACAA AGAGTCTCGA TATCTGTTAC 
AGTGCTGATA AAGAAGTGAC CAACCACATA CTGCACATAA ACTTTAACAC ACTATTAGAA 
AAGAGCACAG ATGTTGCTAG TGGCTGCACA GGGGAAATAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_012962807.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818261.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X7, mRNA.

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CloneID	OXa32641
Clone ID Related Accession (Same CDS sequence)	XM_012962807.2 , XM_012962807.3
Accession Version	XM_012962807.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962807.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GAGAGATGCC CAAAGAGATG 
CTCAGAAGCG GATCTGCCAG GGGGGCGGGG GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818261.1
CDS	639..4934
Translation	MPFHLRLVQPIYVCRLGDPDAQAPRGGNYLIFNSLEDVSSYSLRQIVRQLSDLCRHAENIFSSIHFEASSLGDRTTRLKHRISFLQHNISHLDHKRVTVPVSNLDEESKWTVHYTASCHQQENVFLSSSRPLCVDDLHRQAKVNLKTVLRECDKLRKDGCRSSQYYSQGPTFSTLRLDCTSYQEEENTHESKFSVSFNEEEQILSTKRPKTPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHREMPKEMLRSGSARGAGG

Target ORF information:

RefSeq Version	XM_012962807.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962807.2

ORF Insert Sequence:

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GAGAGATGCC CAAAGAGATG 
CTCAGAAGCG GATCTGCCAG GGGGGCGGGG GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1 ( XM_012962809.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818263.1 Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X8, mRNA.

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CloneID	OXa32642
Clone ID Related Accession (Same CDS sequence)	XM_012962809.3 , XM_012962809.2
Accession Version	XM_012962809.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GTGTGTGCAC ATGTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818263.1
CDS	638..4897
Translation	MPFHLRLVQPIYVCRLGDPDAQAPRGGNYLIFNSLEDVSSYSLRQIVRQLSDLCRHAENIFSSIHFEASSLGDRTTRLKHRISFLQHNISHLDHKRVTVPVSNLDEESKWTVHYTASCHQQENVFLSSSRPLCVDDLHRQAKVNLKTVLRECDKLRKDGCRSSQYYSQGPTFSTLRLDCTSYQEEENTHESKFSVSFNEEEQILSTKRPKTPVLLEFSETLTNWTKSLPLPTPEEKMRQEAQAVQTDVIPINITGENFNRQASFQRSLIYTDTAVRCSKKVKRRKTITGVPNNLQKELAGTGHSDLRRNSVLIPDDFNTLEHCHHSMQFLDTRDFGCQTNKIKIVPPSIRRIRAQKGQGIGILMSTSSGNMSTLSDSAACSSYLNGNLHFRSLPRIGARESLQSLNQKNYYYSNMEDPFTTLPWQLNKLQVDDTNVQLRSNPRSTINQRPESQEVKNSTSEIASSPACMVSPHSSYSTNIIPNAILSYSSEVISIHTPHRGGLLDKKPRVHSSSSNRNGISRDYTSGIKDNSSPGNWRENSSSRNSQISDTKSPISKKRDVPHNVPCKINTGFNNYSFDSDNQSELSYSDGRQRNGSITSSINSADNWLYESRHNDGAPSRKLSSVISGSPVSNMSACSSKGKVDSSSLYFLNHDDFCTSMHIDSGISLTDQSIQNLHYNINIFETKKAANIEVKTGYSDKSLPRSISLKKSKNPPPPPSRTDSLRRPSKKDIQTSGQVLSETLIATLQQSLQLNLKSKSTGSTLQSSHCMDYADPCIMCSCSQSTLSSTSSGKSVIAPNMYTLCTVTPSQSETSSMKSEYADQWSSFIDCSQTPKEQPKSFAKHNLSNPDGLNNYSACELTYGMKTIAPHGYTGSVKVSNSSSEKTYRVTSPSSGYSSQSNTPTTLTPVPLFSKNIYPENGKYKLKPKVPERRSSLVSSVSVSSSSTSLSSNTLDSLQQNMQISTVMLSAHHTLPTPQLTPDMPPPPAMNATNTIFVECSPKCPPPSLEFTRLTADSMDMWLPFSQNTAMSKPKDSLPYPSFPPSLSMMHSMVPSPVPPLNIEMLKIETESFPLRNSESSYFVHNDHSKHELLIPTVPIVTSHALEMVHLRSVKKASQLETKTLREHMSESHLQDETSALLVQPCLVPAASPELNSSESQIENFSIPAQQVSGFKSNAVKLTIGEIEADKAYKEHIEKNTNSAYNGNSSGPKLSPHEKPSMSQSTPNSSLSKEPPLVSKKPKLVLIAPTPQLDLAAEKIAQVTENVRSMPNAFEKDSVSKCCVGEINDRTKDEDIAGPTNGLAHQEQEAWLSPCKAAKNMIFILPSVPEIQNHQQKEEQQSMSDEFNISGNISETENTSCNQNASQDDESAEVFNTETVNSVLWTSSDIMGNVEETTPTRPATTEDLFARIHSVCTCV

Target ORF information:

RefSeq Version	XM_012962809.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 1 (nhsl1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962809.2

ORF Insert Sequence:

ATGCCATTTC ACCTCAGGCT AGTGCAACCA ATTTATGTGT GCCGACTGGG TGACCCAGAT 
GCCCAAGCGC CCAGAGGAGG CAATTATTTG ATCTTTAACT CATTGGAGGA TGTTAGCTCA 
TACTCGCTGC GGCAAATCGT CCGTCAACTT TCCGATCTCT GCAGACATGC TGAGAACATT 
TTTTCCAGTA TTCATTTTGA GGCGTCCTCT TTGGGGGACC GAACTACCCG CTTGAAGCAC 
AGAATTTCGT TTCTACAACA TAATATTAGC CACCTGGACC ATAAAAGGGT TACAGTGCCG 
GTTTCCAATC TAGATGAAGA AAGTAAATGG ACAGTCCATT ACACAGCCTC TTGTCATCAA 
CAAGAGAATG TGTTCCTCTC ATCATCTAGA CCTTTATGTG TAGATGACCT TCACAGGCAA 
GCTAAAGTGA ACCTTAAAAC TGTGCTTAGA GAATGTGACA AGCTTCGGAA GGATGGCTGT 
CGCAGTTCAC AGTACTACTC CCAGGGACCA ACATTCTCGA CACTCAGACT GGATTGCACA 
AGCTATCAGG AAGAGGAAAA TACACATGAG TCAAAGTTTT CTGTTTCATT TAATGAAGAG 
GAACAGATCC TTTCAACCAA AAGGCCCAAG ACTCCTGTGT TGCTTGAGTT TTCTGAAACG 
CTCACTAACT GGACCAAGTC ACTGCCACTG CCTACTCCAG AGGAAAAGAT GCGTCAGGAG 
GCTCAGGCAG TACAGACAGA TGTAATTCCT ATAAACATAA CAGGGGAGAA TTTCAACCGC 
CAGGCCAGCT TTCAGCGGTC ACTTATTTAT ACAGACACTG CCGTAAGATG TTCGAAGAAA 
GTCAAAAGGA GAAAGACTAT AACAGGCGTC CCAAACAACT TACAGAAGGA GCTAGCAGGT 
ACTGGACACT CTGACCTAAG AAGGAATTCA GTGCTCATAC CTGATGATTT TAATACTTTG 
GAACATTGTC ACCACTCAAT GCAATTTCTG GACACCAGAG ATTTTGGCTG CCAAACCAAT 
AAAATAAAAA TAGTGCCACC ATCAATTAGA AGAATCAGAG CACAGAAAGG GCAAGGAATT 
GGAATTCTGA TGTCCACTTC CTCTGGCAAC ATGTCTACTC TAAGTGACTC TGCAGCTTGT 
TCTTCATATC TAAATGGTAA CCTGCACTTC CGTAGTCTGC CCAGGATTGG TGCAAGAGAA 
TCTCTTCAAT CCCTTAACCA AAAAAATTAT TATTACAGCA ACATGGAGGA CCCTTTTACA 
ACTTTGCCTT GGCAGTTAAA CAAACTGCAA GTGGATGATA CTAATGTTCA ATTAAGGAGT 
AACCCTAGGT CAACTATTAA TCAAAGGCCT GAGTCTCAAG AAGTAAAAAA TTCTACTAGT 
GAAATTGCCT CAAGTCCAGC ATGTATGGTC TCGCCTCATT CTTCTTATTC AACAAACATT 
ATTCCAAATG CCATACTTTC ATATTCCTCA GAAGTAATAA GTATTCACAC TCCCCACAGA 
GGTGGACTTC TGGACAAGAA ACCAAGAGTC CACAGTTCAT CTTCTAACAG AAATGGTATT 
AGCAGGGACT ATACTTCTGG CATTAAAGAT AATTCCTCAC CTGGTAACTG GCGTGAAAAC 
AGTTCATCTA GGAACTCACA GATATCAGAT ACAAAGTCAC CTATCAGTAA AAAAAGAGAT 
GTTCCACACA ACGTTCCTTG CAAAATTAAC ACTGGCTTTA ACAACTACTC ATTTGACAGT 
GACAATCAAA GTGAATTAAG CTACTCAGAT GGTCGTCAAA GAAATGGTAG TATAACTAGC 
AGCATTAATA GTGCTGACAA CTGGTTGTAT GAATCTAGGC ACAATGACGG TGCTCCATCT 
AGGAAACTAT CTTCTGTAAT TTCTGGGTCT CCTGTTAGCA ATATGAGCGC CTGCAGTTCT 
AAGGGGAAGG TAGATTCCAG CTCTTTGTAT TTTCTGAACC ATGATGATTT TTGCACATCA 
ATGCATATAG ATTCTGGAAT TAGTTTGACG GACCAAAGCA TTCAGAATCT ACACTATAAT 
ATAAATATCT TTGAAACAAA AAAAGCTGCA AATATAGAAG TGAAAACAGG ATATAGTGAC 
AAATCACTTC CACGTAGCAT ATCTTTAAAG AAATCTAAAA ATCCTCCCCC CCCACCTTCC 
AGGACAGACT CACTTAGAAG GCCATCTAAG AAAGATATTC AGACCAGTGG GCAGGTGCTC 
AGTGAAACTC TTATTGCTAC GCTTCAACAG TCCTTGCAAC TAAACTTGAA GAGTAAGAGC 
ACTGGGTCCA CTTTGCAGTC AAGTCACTGT ATGGATTATG CAGATCCCTG CATCATGTGC 
TCATGTAGCC AGAGTACACT TAGCAGCACT AGTAGTGGAA AATCTGTTAT TGCTCCAAAT 
ATGTACACTC TCTGTACTGT GACACCCTCT CAGAGTGAAA CCAGTAGCAT GAAATCTGAA 
TATGCGGATC AGTGGAGTTC CTTTATTGAC TGTTCACAGA CTCCAAAAGA GCAACCCAAA 
TCATTTGCAA AACACAATCT AAGTAATCCT GATGGTCTTA ATAATTACAG TGCATGTGAG 
CTGACCTATG GAATGAAGAC CATAGCACCA CATGGGTACA CTGGATCAGT TAAAGTTAGT 
AATTCCTCTT CAGAAAAAAC ATACAGAGTT ACATCACCAT CTAGTGGATA TTCAAGTCAG 
TCAAACACTC CCACCACATT GACACCAGTG CCTCTTTTCT CAAAGAATAT ATATCCAGAA 
AATGGAAAAT ACAAGTTAAA ACCAAAAGTA CCTGAAAGAC GATCTTCATT GGTATCCTCG 
GTTTCAGTTT CATCTTCCTC TACTTCCCTT TCTTCAAATA CATTGGACTC TCTGCAGCAG 
AACATGCAAA TTTCAACTGT TATGTTGTCA GCCCATCACA CTCTTCCAAC CCCACAACTC 
ACTCCTGACA TGCCCCCACC CCCAGCAATG AATGCAACCA ACACAATTTT TGTCGAGTGT 
TCACCAAAAT GTCCACCTCC TTCCCTTGAA TTTACAAGGC TAACAGCGGA TAGCATGGAT 
ATGTGGCTTC CCTTCTCTCA AAACACTGCC ATGAGTAAAC CTAAGGACAG TCTTCCATAT 
CCTTCCTTTC CACCTTCTCT TTCCATGATG CACAGCATGG TCCCATCACC TGTACCTCCA 
CTTAACATTG AAATGCTGAA AATTGAAACA GAATCATTTC CTCTCAGAAA TTCTGAAAGC 
AGTTATTTTG TACATAATGA TCACAGTAAA CATGAACTGT TGATACCAAC TGTTCCTATA 
GTTACTTCTC ATGCACTAGA AATGGTTCAT TTGAGATCTG TGAAAAAAGC ATCACAACTG 
GAAACTAAAA CATTAAGAGA ACACATGTCA GAATCCCACC TACAGGATGA GACATCCGCC 
TTATTGGTAC AGCCATGTTT AGTACCAGCT GCTTCTCCAG AACTAAATTC TAGTGAAAGC 
CAAATAGAAA ATTTCAGCAT TCCTGCCCAG CAAGTCTCTG GTTTTAAAAG CAATGCAGTG 
AAACTAACTA TTGGTGAAAT AGAAGCAGAC AAGGCATATA AAGAGCACAT AGAAAAAAAT 
ACAAATTCAG CATACAATGG AAACTCATCA GGGCCCAAAT TATCTCCTCA TGAGAAACCA 
AGTATGTCAC AAAGCACACC AAATTCATCT CTTAGCAAGG AGCCGCCTCT TGTTTCAAAG 
AAGCCTAAAC TTGTACTTAT TGCCCCAACG CCACAACTTG ATTTGGCAGC AGAAAAAATA 
GCTCAAGTCA CTGAAAATGT GAGAAGCATG CCAAATGCCT TTGAAAAAGA TTCAGTTAGC 
AAATGTTGTG TGGGGGAGAT AAATGACAGA ACTAAAGATG AAGACATAGC AGGTCCAACC 
AATGGTTTGG CTCACCAAGA ACAAGAAGCC TGGTTATCCC CATGCAAAGC TGCTAAAAAT 
ATGATTTTCA TACTACCATC AGTTCCAGAA ATTCAGAATC ATCAGCAAAA GGAAGAGCAG 
CAATCCATGT CAGATGAATT CAACATTAGT GGTAACATTT CTGAGACTGA GAATACAAGC 
TGCAACCAGA ATGCATCTCA AGATGATGAG AGTGCTGAAG TGTTTAATAC AGAGACGGTG 
AATTCAGTTC TCTGGACAAG CAGCGACATT ATGGGAAATG TAGAAGAGAC CACTCCAACA 
AGGCCTGCTA CAACTGAGGA TCTTTTTGCT CGTATCCATA GTGTGTGCAC ATGTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	nhsl1
Entrez Gene ID	100216152
Full Name	NHS like 1
General protein information	Preferred Names NHS like 1 Names NHS-like protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Pan troglodytes (chimpanzee)	NHSL1	XP_001171693.2
Mus musculus (house mouse)	Nhsl1	NP_775566.3
Danio rerio (zebrafish)	nhsl1b	XP_005160821.1
Canis lupus familiaris (dog)	NHSL1	XP_005615510.1
Rattus norvegicus (Norway rat)	Nhsl1	XP_006227729.1
	NHSL1	XP_001095123.2
Xenopus tropicalis (tropical clawed frog)	nhsl1	NP_001135597.1
Homo sapiens (human)	NHSL1	NP_065197.1
Bos taurus (cattle)	NHSL1	NP_001178467.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

nhsl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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