nup205 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nup205
Entrez Gene ID	100216151
Full Name	nucleoporin 205kDa
Synonyms	xnup205
General protein information	Preferred Names nucleoporin 205kDa Names nuclear pore complex protein Nup205
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142124.1	NP_001135596.1	nuclear pore complex protein Nup205
XM_012958683.3	XP_012814137.1	nuclear pore complex protein Nup205 isoform X1
XM_012958683.2	XP_012814137.1	nuclear pore complex protein Nup205 isoform X1
XM_012958684.2	XP_012814138.1	nuclear pore complex protein Nup205 isoform X2
XM_012958684.3	XP_012814138.1	nuclear pore complex protein Nup205 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NUP205	XP_416176.2
Arabidopsis thaliana (thale cress)	AT5G51200	NP_199933.5
Pan troglodytes (chimpanzee)	NUP205	XP_527899.3
Rattus norvegicus (Norway rat)	Nup205	NP_001102090.1
Drosophila melanogaster (fruit fly)	Nup205	NP_728308.1
	NUP205	XP_001105911.1
Bos taurus (cattle)	NUP205	NP_001124403.1
Mus musculus (house mouse)	Nup205	NP_081789.1
Danio rerio (zebrafish)	nup205	NP_001003859.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008090	XP_317367.4
Xenopus tropicalis (tropical clawed frog)	nup205	NP_001135596.1
Homo sapiens (human)	NUP205	NP_055950.1
Canis lupus familiaris (dog)	NUP205	XP_005629638.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nup205 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nup205 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa53358	XM_012958683.3 Latest version!	Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa53359	XM_012958684.3 Latest version!	Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa21731	NM_001142124.1 Latest version!	Xenopus tropicalis nucleoporin 205kDa (nup205), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa32633	XM_012958683.2 Latest version!	Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa32634	XM_012958684.2 Latest version!	Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nup205 ( XM_012958683.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814137.1 Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa53358
Clone ID Related Accession (Same CDS sequence)	XM_012958683.3
Accession Version	XM_012958683.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear pore complex protein Nup205 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958683.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC AGTTGGCGCT AAATTCAGAA GCCAGCCTGT GGGGCCCTTA TAGAGAAATA 
TGGCAGACCG TGGTGGGGGC GCTTGTGAAG AGGCAACCGG AAGCCATTCA TTCGCTCGAT 
ATCGTCTTAA AGAAATATAA ACCGGACTTC ATCTCTCTCT TCAAAAATCC ACCAAAGAGC 
GCTCAGCAAC ATGAAAGAGT GCAGAAAGCC AGCAGCGAGG GGATTCCTAT TAAAGGGACG 
CAGCGGACCC GGATCCTGGA AGGACAGCTC ATTAAGGAAG CCTTCATCCT TAGCGACCTG 
TATAATATCG GAGAGGTGGC AGCCGTAGAA CTTCTACTTT TAGGTGAGCA GCAGCAGCCT 
ACCTTCCATG GCCTGACGAG GGGCTTGGTA GCCATACTGT TGTACTGGGA TGGAAAGAGC 
TGCATGGCTG ATTCCTTATT ACACCTCATC CAGGCAAGAA AAGGCAGGAC CTTCACCTTG 
AATCACAGCC CCGAGCTTGT GTCCATGGTA ACTCGTTTCA CCGATGATCT AATGGAGCAA 
GGCTTGACTA ATAAAATCCT CACCTTGATC TCTCAAATTG ACGTGAACAA TGAGTTTGAC 
AAGTTGCTGA AGGATCGGGG CCTGGGCAAC GAGAAGCATC GGAAAGAGGT TTCTGATCTG 
ATAAAGGAAT GTCGGCAGTC GCTCGCACAC AGCCTGTATT CATGGAGTTG CCAGACACCA 
CTAAACAAAG AAGATACACT ACTTTTGATT GGATACTTGG AAAAAGTAAC AGTAGAAGGA 
GATGGCTCAT TGGACAAAGT GAACTTGACC CTACTGATGT CACTTCTCTA TTGCTTGGAT 
GTTGGCTTTT TGGAGCAAGC GACAGATGAT CGAGAAGAAC TGATAAAGCA GACCAGCATG 
TTCATGGACA GACAGTACAT TGCCGCCATC CATAACCGCC TGCACGACAC TCAGCCGTGG 
AAGCTGCCGG GAATGCAGGC TACGGTCAGA CTCGCTTGGG CCCTGGCTCT TCGAGGGATC 
TCTCAGTTCT CAGAAGTCTT AGAATTTTCA GAAGCAGACG AGCCAATGGC AGAGCTAGCG 
ATCAGCGGCA ATGTCTTCCT CTTCCTCACG GAGGCAGTGG TGGGATCCGA AAGCTTCTGT 
GCAGATGAGT TCTTTATACG AAGGATTCAC AAACTCGTCA CCGATTTCCT TACTCTCATG 
CCCATGAAAG TAAAGCAGCT GAGAAATCGT GCAGAAGAGG ATGCCCGACT GATCCATATG 
AGCATGCAGA TGGGAAATGA GCCCCCTGCT TCTCTGCGTA GGGATTTGGA ACATCTGCTC 
TTACTGATCG GTGAGCTCTA CCGGATAGAC CCATTCCACT TGGAACTGGC GTTGGAATAC 
TGGTGCCCCG CAGAGCCTCT CCAGTCGACA TCCTTGATGG GATCCTTTCT TGGTGTGGCC 
CACCAGAGAC CACCTCAACG TCAGGTCGTC TTGTCCAAGT TCGTGAGACA GATGAGTGAC 
CTGCTGCCAG CCACGCTCTA CATACCTTAT CTTAAGATGC TCAGGGGCCT TGCAAGTGGC 
CCTCAGTGCG CCCATTACTG TTTCAGTCTG CTAAAGGCCA ATGGGGGCAG CAGCGCAGAG 
AGCTTGCAAG CTGCAGGGGG TAGCCCAGTG TCCTGGGACC ACTTCTTCCA CTCTTTGATG 
CTGTACCACG AGCACTTGCG GAGGGATCTA CCAAGCACAG ACAGTATACA CCTGCGTCAC 
CCTCCTCTCC GTGGTATCAC CCAGCGAGAG CTCGATGGCC TGATTGCCTG CTTGCAGCTC 
ACCCTTACCA TCGTCGATTG GAGTGAAAGT GCCCGCCTTG CTCTGTGTGA GCATGCCCAG 
TGGATGCCAG TGGTCGTTAT TCTTGGCCTC CTGCAGTGCA GCATCCCACC ACTCCTTAAG 
GCTGAGCTCC TGAAGACACT GGCTGCATTT GGCAAATCCC CAGAGATCGC AGCATCCCTC 
TGGCAATCCC TCGAATATAC ACAGATTTTG CAGACTGTAA GAACCCCAGG GCTACGGCAA 
GGAGTCGGGA TTGAGGTGGA ACTGAATGAG ATCGAGTCTC GGTGTGAGGA GTACCCCCTG 
ACCAGAGCAT TCTGCCAGCT CATTAGCACT TTAGTGGAGA GCTCCTTCCC CACCAATCTG 
GGAGCGGGTT TGAGAGCACC CGGCTTTGAG CCATACCTCC AGTTTCTACG GGACACTGTG 
TTCCTTCGGT TCCGCACTAG AGCCTACAGG AGAGGCGCAG AAAAGTGGGA GGTAGCAGAG 
GCTGTACTGG ATGTCTTCTA CAAGCTTTTG AAGGACTATG AGCCCCAGCC TGAAGACTTT 
GTGGACCAGT ATGTGGAACT CCAAGGAGAA GAAAGAGTTG CTTTCAAGCC CCCGGGGTTC 
AGCCTCATGC ATCACCTTCT CAATGATTCT CCGATGCTGG AGCTCTGCCT CAGCCTCATG 
GAAGAGGGGG TCACCCAGCT GGACACTTAC GCCCCTTTTC CAGGCAAGAA ACACCTAGAG 
AAAGCAGTGG CTTATTGTTT CATGCTCCTC AATCAGACTC TGCAGAAGGA GAATCGCTTC 
ATGGATTTGT TGAGGGAAAG TCACCTGTCG TTGATTGTGA CCCCTCTGGA GCAGCTCCTG 
CAGGGAATTA ACCCACGATC CAAGAAAGCT GATAATGTCA TCAATATTGC TCGATATCTC 
TACCATGGGA ACAGCAATCC CGAGCTTGCC TTTGAAAGTG CAAAGATCTT ATGCAGCATC 
GCCTGTAACT CCAAGATCCA AGAAAAGATT GTAGGAGACT TTACCCAAGA TCAGAACGTG 
ACCCAGAAGC TGATGGTTGG GTTTGTTTCA TGCCTGGACA GCGAGGAAGC AGAGGAGTTA 
CTGGATTCAG AGAAAGAGGT GGAGGACCAA GCCAAGCAGA CGAACATCCG GTACATGACA 
AAGATTCACA TCCTCAATCT TCTGATCACC TCCCTGGAAA TGAAAGCCCC CAACCTTGCC 
ATGTTCCTTC TCGGCTACGA ACTGAAGAAG CCAGTCAGCA CGACCAACCT GCAGGACTCT 
GGTGTCCTGG GCTGCCCGCG GACCTGCCTT CACTCCATTT TAGACATCTT GAGAAAGGGG 
ACTGATGGGC GGGCTGGACC CATAGCCATA TGGGATACTC CTCATCTTGC TGAACTGTGT 
TACCAGGTGA TCTACCAGCT GTGCGCTTGT TCAGATACCT CTGGCCCCAC CATGAGATAC 
CTACGGACCA GCCAGGATTT CCTCTTCACT CAGCTTCAGC ACCTGCCCTT CTCTGTGGAA 
GAGTATGAGA TCTCCGCCAT GAATCAGATG TCCTGGCTGA TGAAGACGGC GTCCATAGAA 
CTGCGCATCA CCTCCCTAAA CCGCCAACGT TCCCACACGC AGAGGCTCCT TCACCTGCTG 
CTTGACGACA TGCCCACCAA ACCGTATTCA GCTGATGGCG AAGGAGGAAT GGAGGACGAA 
AGCCGCAGCC TCAGTGGATT TCTGCACTTT GACACTACCT CAAAAGTCCG CCGGAAAATC 
CTCCGCATCC TGGACTCCAT ACATTTCAGC AATGAGATCC CGGATCCCCT GCAGTTGGAT 
TTCTTCGATC GCAGCCAGAT AGAGCAGGTT ATTGCTAACT GCGAGCACAA GAACCGCAGA 
GGGCAGACCG TCTGCAACGT CAAGCTTCTC CATCGGGTTC TGGTTGCAGA AGTGAATGCC 
CTTCAGGGAA TGGCTGCTAT TGGTCAGAGG CCGCTGCTTA TGGAGGAAAT CAACACCATC 
CTTCAGTACG TGGTGGAAAG GAACAAGCTG CTGCAGTGCC TCCACGCCAA ACGCCATGCG 
CTGGAATCAT GGCGGCAACT TGTGGAGATC ATACTGACCG CCTGTCCCCA GGACCTCATT 
CCGACTGAGC ACAGACAGCT CATCATACGA GACCTGCTGC AAGATCTGCA TGATAAGATC 
CTCGACGACG ACGCTGCCCA AGAACTAATG CCCATTGTCG CCGGGGCAGT CTTCACCCTC 
ACCACTCATC TCAGCCAATC TGTACGTACC GAGCTGAAAC AGCCGATGGC TGCCGCTGGG 
CTGGGCCAGT CGCAATATGT GCAAATGCTG GACGGCTCAT TCGCTGCCCC TCCTGGGACA 
GAAAACCTCT CCGCTGGTTT TGCATCCATT GGAGACTCCT CCCTTCACAT GATTCTCAGG 
AATCTGCTCG AGTTCATTCT AAAAACAGGA GGTGGGTTCC AACGGGTCAG GGCCCACCTG 
TACGGATCGC TGCTGTATTA CCTGCAGATA GCTCAGAGAC CAGATGAACC AGATACTCTG 
GAATCCGCAC ACAAGACTAT GTGGGAGCGA CTGACGGCCC CGGAGGATGT ATTCAGCAAG 
CTGCAGCGAG ACAACCTGTC CATATTCGAG AGCTACGGCA CCGCCCTTAT GGAAGTGGTT 
TGCCGGGACG CCTGTGACGG ACATGACATT GGGAGGATGC TGGCCCTGGC ACTGCTTGAC 
CGGATTGTCT CTGTGGATAG GCAACAGTGG CTGCTTTATC TCTCCAACAG TGGCTACCTG 
AAGGTCCTTG TGGATAGCCT GGCAGAGGAC GATATGGTCC TAAGGAACCT GCTAACTCCT 
CAGGCGCCTC TTCTAAAGGC TCTCTATATC TATGAGTCGA AAATGGCATT TCTGACCCGG 
GTGGCTAAAA GCCAGCAGGG AGCCCTGGAG CTGCTGAGGA GTGGGGTTAT CGTCAGGCTG 
GCACAGTGCC AAGTATATGA TATGCGCCCG GAGACTGACC CCCATGGAGT GTTTGGCATG 
AGAGAGACCC CGGTGTTTAT CCCCGCCCCG GTGGAACGAT ACCGGCAAAT CCTGTTACCC 
GCTCTCCAGA TCTGTCAGCT CATCCTGACC TCCAGCACGG CACAGCACTT ACAGGCCGCC 
GGACAGGTCT TGCAGTTCCT GATCGCTCAT TCTGATACCA TCCAGGCAAT TCTCCGGAGC 
CAGGAGGGGA GTCTGGGTTC CTTGCAGGAG TTGGCTTTGC TTACAGGCAT CATCAGCAAG 
GCCGCACTAC CCGGTGTTCT CAACGAGCTC GATATCGGTT TAAACGAAGG GTCCATGATG 
GAGTTACAAG GACATATTGG CCGCTTCCAG CGCCAGTGTC TTGCGTTATT AAACCGCTTT 
GGGGGGTCCG ACCGACTTCG GCAGCTCAGT CTGCAGGATG ATTCCTCCCA GCCGGACGGT 
GTGAGCAAGA GGGACGACAT GGAGTTGGCC ATGCAACAGA TCTGCTCCAA CGTGATGGAG 
TACTGCCAGA CGCTTATGAT ACAGAACTCG CCAAGCTTCC AGCAGACTGT GTGTCTCTTC 
ACCCCCAACC TCACGGAGAG CTCCAGCCGA GATGGAACAC GGCAGGATTC CCAGGTGTCC 
ATTCTGCCGT CATGGCGGTT GCCAGGCTTG GGTATAGTGA TCCACCTTCT GAAGCAGAGC 
GCCAATAACT TCTTCACTTA CTATGATATC CACCGGCAGA GCGTCAGCAA GCTGCAGAAC 
GTGGAGCAAC TCCCGCCCGA TGAGATTAAA GAGCTGTGCC AGTCTGAGAT GCCGGTTGGC 
GTAGATAAAA TCTCGACGAC GCAGAAGTAC GCGCTGGCAC GGCGGCGACT CGTGAAGCTT 
ATCAACAGCA GAGCCAAACT GCTTTCTCTT TGTAGTTATA TCATAGAAAC GTGTCTGTTC 
ATTCTGTGGC GCCACTTGGA ACATTACCTT CTGCACTGCA CCACCAGCGA CTCCCAGGAC 
CCCGTCTTCT CGAATACGAC CTTCGGCAAC AGAAGGTTTC AAGATACAAT TAACACTGAT 
CCTAACATGG ACCTGAGGAA CCTGAGACAG AATAAAGTCA GCCAACAGGA AATTGATATG 
CTGCTAAGAG AAGGCGCCAA TAGTTTCGGG GAGTCCCTGC AGAAGAGACT GTTAGACATC 
GAATCATTGT ACTGCAAGGT GCGATCCCGT CACAGCTTCA TCCAGGCGCT GGTCAGGCGG 
ATCCGGGGCC TCCTGCGAGT ATCAAGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814137.1
CDS	237..6269
Translation	MAAQLALNSEASLWGPYREIWQTVVGALVKRQPEAIHSLDIVLKKYKPDFISLFKNPPKSAQQHERVQKASSEGIPIKGTQRTRILEGQLIKEAFILSDLYNIGEVAAVELLLLGEQQQPTFHGLTRGLVAILLYWDGKSCMADSLLHLIQARKGRTFTLNHSPELVSMVTRFTDDLMEQGLTNKILTLISQIDVNNEFDKLLKDRGLGNEKHRKEVSDLIKECRQSLAHSLYSWSCQTPLNKEDTLLLIGYLEKVTVEGDGSLDKVNLTLLMSLLYCLDVGFLEQATDDREELIKQTSMFMDRQYIAAIHNRLHDTQPWKLPGMQATVRLAWALALRGISQFSEVLEFSEADEPMAELAISGNVFLFLTEAVVGSESFCADEFFIRRIHKLVTDFLTLMPMKVKQLRNRAEEDARLIHMSMQMGNEPPASLRRDLEHLLLLIGELYRIDPFHLELALEYWCPAEPLQSTSLMGSFLGVAHQRPPQRQVVLSKFVRQMSDLLPATLYIPYLKMLRGLASGPQCAHYCFSLLKANGGSSAESLQAAGGSPVSWDHFFHSLMLYHEHLRRDLPSTDSIHLRHPPLRGITQRELDGLIACLQLTLTIVDWSESARLALCEHAQWMPVVVILGLLQCSIPPLLKAELLKTLAAFGKSPEIAASLWQSLEYTQILQTVRTPGLRQGVGIEVELNEIESRCEEYPLTRAFCQLISTLVESSFPTNLGAGLRAPGFEPYLQFLRDTVFLRFRTRAYRRGAEKWEVAEAVLDVFYKLLKDYEPQPEDFVDQYVELQGEERVAFKPPGFSLMHHLLNDSPMLELCLSLMEEGVTQLDTYAPFPGKKHLEKAVAYCFMLLNQTLQKENRFMDLLRESHLSLIVTPLEQLLQGINPRSKKADNVINIARYLYHGNSNPELAFESAKILCSIACNSKIQEKIVGDFTQDQNVTQKLMVGFVSCLDSEEAEELLDSEKEVEDQAKQTNIRYMTKIHILNLLITSLEMKAPNLAMFLLGYELKKPVSTTNLQDSGVLGCPRTCLHSILDILRKGTDGRAGPIAIWDTPHLAELCYQVIYQLCACSDTSGPTMRYLRTSQDFLFTQLQHLPFSVEEYEISAMNQMSWLMKTASIELRITSLNRQRSHTQRLLHLLLDDMPTKPYSADGEGGMEDESRSLSGFLHFDTTSKVRRKILRILDSIHFSNEIPDPLQLDFFDRSQIEQVIANCEHKNRRGQTVCNVKLLHRVLVAEVNALQGMAAIGQRPLLMEEINTILQYVVERNKLLQCLHAKRHALESWRQLVEIILTACPQDLIPTEHRQLIIRDLLQDLHDKILDDDAAQELMPIVAGAVFTLTTHLSQSVRTELKQPMAAAGLGQSQYVQMLDGSFAAPPGTENLSAGFASIGDSSLHMILRNLLEFILKTGGGFQRVRAHLYGSLLYYLQIAQRPDEPDTLESAHKTMWERLTAPEDVFSKLQRDNLSIFESYGTALMEVVCRDACDGHDIGRMLALALLDRIVSVDRQQWLLYLSNSGYLKVLVDSLAEDDMVLRNLLTPQAPLLKALYIYESKMAFLTRVAKSQQGALELLRSGVIVRLAQCQVYDMRPETDPHGVFGMRETPVFIPAPVERYRQILLPALQICQLILTSSTAQHLQAAGQVLQFLIAHSDTIQAILRSQEGSLGSLQELALLTGIISKAALPGVLNELDIGLNEGSMMELQGHIGRFQRQCLALLNRFGGSDRLRQLSLQDDSSQPDGVSKRDDMELAMQQICSNVMEYCQTLMIQNSPSFQQTVCLFTPNLTESSSRDGTRQDSQVSILPSWRLPGLGIVIHLLKQSANNFFTYYDIHRQSVSKLQNVEQLPPDEIKELCQSEMPVGVDKISTTQKYALARRRLVKLINSRAKLLSLCSYIIETCLFILWRHLEHYLLHCTTSDSQDPVFSNTTFGNRRFQDTINTDPNMDLRNLRQNKVSQQEIDMLLREGANSFGESLQKRLLDIESLYCKVRSRHSFIQALVRRIRGLLRVSRA

Target ORF information:

RefSeq Version	XM_012958683.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958683.3

ORF Insert Sequence:

ATGGCGGCGC AGTTGGCGCT AAATTCAGAA GCCAGCCTGT GGGGCCCTTA TAGAGAAATA 
TGGCAGACCG TGGTGGGGGC GCTTGTGAAG AGGCAACCGG AAGCCATTCA TTCGCTCGAT 
ATCGTCTTAA AGAAATATAA ACCGGACTTC ATCTCTCTCT TCAAAAATCC ACCAAAGAGC 
GCTCAGCAAC ATGAAAGAGT GCAGAAAGCC AGCAGCGAGG GGATTCCTAT TAAAGGGACG 
CAGCGGACCC GGATCCTGGA AGGACAGCTC ATTAAGGAAG CCTTCATCCT TAGCGACCTG 
TATAATATCG GAGAGGTGGC AGCCGTAGAA CTTCTACTTT TAGGTGAGCA GCAGCAGCCT 
ACCTTCCATG GCCTGACGAG GGGCTTGGTA GCCATACTGT TGTACTGGGA TGGAAAGAGC 
TGCATGGCTG ATTCCTTATT ACACCTCATC CAGGCAAGAA AAGGCAGGAC CTTCACCTTG 
AATCACAGCC CCGAGCTTGT GTCCATGGTA ACTCGTTTCA CCGATGATCT AATGGAGCAA 
GGCTTGACTA ATAAAATCCT CACCTTGATC TCTCAAATTG ACGTGAACAA TGAGTTTGAC 
AAGTTGCTGA AGGATCGGGG CCTGGGCAAC GAGAAGCATC GGAAAGAGGT TTCTGATCTG 
ATAAAGGAAT GTCGGCAGTC GCTCGCACAC AGCCTGTATT CATGGAGTTG CCAGACACCA 
CTAAACAAAG AAGATACACT ACTTTTGATT GGATACTTGG AAAAAGTAAC AGTAGAAGGA 
GATGGCTCAT TGGACAAAGT GAACTTGACC CTACTGATGT CACTTCTCTA TTGCTTGGAT 
GTTGGCTTTT TGGAGCAAGC GACAGATGAT CGAGAAGAAC TGATAAAGCA GACCAGCATG 
TTCATGGACA GACAGTACAT TGCCGCCATC CATAACCGCC TGCACGACAC TCAGCCGTGG 
AAGCTGCCGG GAATGCAGGC TACGGTCAGA CTCGCTTGGG CCCTGGCTCT TCGAGGGATC 
TCTCAGTTCT CAGAAGTCTT AGAATTTTCA GAAGCAGACG AGCCAATGGC AGAGCTAGCG 
ATCAGCGGCA ATGTCTTCCT CTTCCTCACG GAGGCAGTGG TGGGATCCGA AAGCTTCTGT 
GCAGATGAGT TCTTTATACG AAGGATTCAC AAACTCGTCA CCGATTTCCT TACTCTCATG 
CCCATGAAAG TAAAGCAGCT GAGAAATCGT GCAGAAGAGG ATGCCCGACT GATCCATATG 
AGCATGCAGA TGGGAAATGA GCCCCCTGCT TCTCTGCGTA GGGATTTGGA ACATCTGCTC 
TTACTGATCG GTGAGCTCTA CCGGATAGAC CCATTCCACT TGGAACTGGC GTTGGAATAC 
TGGTGCCCCG CAGAGCCTCT CCAGTCGACA TCCTTGATGG GATCCTTTCT TGGTGTGGCC 
CACCAGAGAC CACCTCAACG TCAGGTCGTC TTGTCCAAGT TCGTGAGACA GATGAGTGAC 
CTGCTGCCAG CCACGCTCTA CATACCTTAT CTTAAGATGC TCAGGGGCCT TGCAAGTGGC 
CCTCAGTGCG CCCATTACTG TTTCAGTCTG CTAAAGGCCA ATGGGGGCAG CAGCGCAGAG 
AGCTTGCAAG CTGCAGGGGG TAGCCCAGTG TCCTGGGACC ACTTCTTCCA CTCTTTGATG 
CTGTACCACG AGCACTTGCG GAGGGATCTA CCAAGCACAG ACAGTATACA CCTGCGTCAC 
CCTCCTCTCC GTGGTATCAC CCAGCGAGAG CTCGATGGCC TGATTGCCTG CTTGCAGCTC 
ACCCTTACCA TCGTCGATTG GAGTGAAAGT GCCCGCCTTG CTCTGTGTGA GCATGCCCAG 
TGGATGCCAG TGGTCGTTAT TCTTGGCCTC CTGCAGTGCA GCATCCCACC ACTCCTTAAG 
GCTGAGCTCC TGAAGACACT GGCTGCATTT GGCAAATCCC CAGAGATCGC AGCATCCCTC 
TGGCAATCCC TCGAATATAC ACAGATTTTG CAGACTGTAA GAACCCCAGG GCTACGGCAA 
GGAGTCGGGA TTGAGGTGGA ACTGAATGAG ATCGAGTCTC GGTGTGAGGA GTACCCCCTG 
ACCAGAGCAT TCTGCCAGCT CATTAGCACT TTAGTGGAGA GCTCCTTCCC CACCAATCTG 
GGAGCGGGTT TGAGAGCACC CGGCTTTGAG CCATACCTCC AGTTTCTACG GGACACTGTG 
TTCCTTCGGT TCCGCACTAG AGCCTACAGG AGAGGCGCAG AAAAGTGGGA GGTAGCAGAG 
GCTGTACTGG ATGTCTTCTA CAAGCTTTTG AAGGACTATG AGCCCCAGCC TGAAGACTTT 
GTGGACCAGT ATGTGGAACT CCAAGGAGAA GAAAGAGTTG CTTTCAAGCC CCCGGGGTTC 
AGCCTCATGC ATCACCTTCT CAATGATTCT CCGATGCTGG AGCTCTGCCT CAGCCTCATG 
GAAGAGGGGG TCACCCAGCT GGACACTTAC GCCCCTTTTC CAGGCAAGAA ACACCTAGAG 
AAAGCAGTGG CTTATTGTTT CATGCTCCTC AATCAGACTC TGCAGAAGGA GAATCGCTTC 
ATGGATTTGT TGAGGGAAAG TCACCTGTCG TTGATTGTGA CCCCTCTGGA GCAGCTCCTG 
CAGGGAATTA ACCCACGATC CAAGAAAGCT GATAATGTCA TCAATATTGC TCGATATCTC 
TACCATGGGA ACAGCAATCC CGAGCTTGCC TTTGAAAGTG CAAAGATCTT ATGCAGCATC 
GCCTGTAACT CCAAGATCCA AGAAAAGATT GTAGGAGACT TTACCCAAGA TCAGAACGTG 
ACCCAGAAGC TGATGGTTGG GTTTGTTTCA TGCCTGGACA GCGAGGAAGC AGAGGAGTTA 
CTGGATTCAG AGAAAGAGGT GGAGGACCAA GCCAAGCAGA CGAACATCCG GTACATGACA 
AAGATTCACA TCCTCAATCT TCTGATCACC TCCCTGGAAA TGAAAGCCCC CAACCTTGCC 
ATGTTCCTTC TCGGCTACGA ACTGAAGAAG CCAGTCAGCA CGACCAACCT GCAGGACTCT 
GGTGTCCTGG GCTGCCCGCG GACCTGCCTT CACTCCATTT TAGACATCTT GAGAAAGGGG 
ACTGATGGGC GGGCTGGACC CATAGCCATA TGGGATACTC CTCATCTTGC TGAACTGTGT 
TACCAGGTGA TCTACCAGCT GTGCGCTTGT TCAGATACCT CTGGCCCCAC CATGAGATAC 
CTACGGACCA GCCAGGATTT CCTCTTCACT CAGCTTCAGC ACCTGCCCTT CTCTGTGGAA 
GAGTATGAGA TCTCCGCCAT GAATCAGATG TCCTGGCTGA TGAAGACGGC GTCCATAGAA 
CTGCGCATCA CCTCCCTAAA CCGCCAACGT TCCCACACGC AGAGGCTCCT TCACCTGCTG 
CTTGACGACA TGCCCACCAA ACCGTATTCA GCTGATGGCG AAGGAGGAAT GGAGGACGAA 
AGCCGCAGCC TCAGTGGATT TCTGCACTTT GACACTACCT CAAAAGTCCG CCGGAAAATC 
CTCCGCATCC TGGACTCCAT ACATTTCAGC AATGAGATCC CGGATCCCCT GCAGTTGGAT 
TTCTTCGATC GCAGCCAGAT AGAGCAGGTT ATTGCTAACT GCGAGCACAA GAACCGCAGA 
GGGCAGACCG TCTGCAACGT CAAGCTTCTC CATCGGGTTC TGGTTGCAGA AGTGAATGCC 
CTTCAGGGAA TGGCTGCTAT TGGTCAGAGG CCGCTGCTTA TGGAGGAAAT CAACACCATC 
CTTCAGTACG TGGTGGAAAG GAACAAGCTG CTGCAGTGCC TCCACGCCAA ACGCCATGCG 
CTGGAATCAT GGCGGCAACT TGTGGAGATC ATACTGACCG CCTGTCCCCA GGACCTCATT 
CCGACTGAGC ACAGACAGCT CATCATACGA GACCTGCTGC AAGATCTGCA TGATAAGATC 
CTCGACGACG ACGCTGCCCA AGAACTAATG CCCATTGTCG CCGGGGCAGT CTTCACCCTC 
ACCACTCATC TCAGCCAATC TGTACGTACC GAGCTGAAAC AGCCGATGGC TGCCGCTGGG 
CTGGGCCAGT CGCAATATGT GCAAATGCTG GACGGCTCAT TCGCTGCCCC TCCTGGGACA 
GAAAACCTCT CCGCTGGTTT TGCATCCATT GGAGACTCCT CCCTTCACAT GATTCTCAGG 
AATCTGCTCG AGTTCATTCT AAAAACAGGA GGTGGGTTCC AACGGGTCAG GGCCCACCTG 
TACGGATCGC TGCTGTATTA CCTGCAGATA GCTCAGAGAC CAGATGAACC AGATACTCTG 
GAATCCGCAC ACAAGACTAT GTGGGAGCGA CTGACGGCCC CGGAGGATGT ATTCAGCAAG 
CTGCAGCGAG ACAACCTGTC CATATTCGAG AGCTACGGCA CCGCCCTTAT GGAAGTGGTT 
TGCCGGGACG CCTGTGACGG ACATGACATT GGGAGGATGC TGGCCCTGGC ACTGCTTGAC 
CGGATTGTCT CTGTGGATAG GCAACAGTGG CTGCTTTATC TCTCCAACAG TGGCTACCTG 
AAGGTCCTTG TGGATAGCCT GGCAGAGGAC GATATGGTCC TAAGGAACCT GCTAACTCCT 
CAGGCGCCTC TTCTAAAGGC TCTCTATATC TATGAGTCGA AAATGGCATT TCTGACCCGG 
GTGGCTAAAA GCCAGCAGGG AGCCCTGGAG CTGCTGAGGA GTGGGGTTAT CGTCAGGCTG 
GCACAGTGCC AAGTATATGA TATGCGCCCG GAGACTGACC CCCATGGAGT GTTTGGCATG 
AGAGAGACCC CGGTGTTTAT CCCCGCCCCG GTGGAACGAT ACCGGCAAAT CCTGTTACCC 
GCTCTCCAGA TCTGTCAGCT CATCCTGACC TCCAGCACGG CACAGCACTT ACAGGCCGCC 
GGACAGGTCT TGCAGTTCCT GATCGCTCAT TCTGATACCA TCCAGGCAAT TCTCCGGAGC 
CAGGAGGGGA GTCTGGGTTC CTTGCAGGAG TTGGCTTTGC TTACAGGCAT CATCAGCAAG 
GCCGCACTAC CCGGTGTTCT CAACGAGCTC GATATCGGTT TAAACGAAGG GTCCATGATG 
GAGTTACAAG GACATATTGG CCGCTTCCAG CGCCAGTGTC TTGCGTTATT AAACCGCTTT 
GGGGGGTCCG ACCGACTTCG GCAGCTCAGT CTGCAGGATG ATTCCTCCCA GCCGGACGGT 
GTGAGCAAGA GGGACGACAT GGAGTTGGCC ATGCAACAGA TCTGCTCCAA CGTGATGGAG 
TACTGCCAGA CGCTTATGAT ACAGAACTCG CCAAGCTTCC AGCAGACTGT GTGTCTCTTC 
ACCCCCAACC TCACGGAGAG CTCCAGCCGA GATGGAACAC GGCAGGATTC CCAGGTGTCC 
ATTCTGCCGT CATGGCGGTT GCCAGGCTTG GGTATAGTGA TCCACCTTCT GAAGCAGAGC 
GCCAATAACT TCTTCACTTA CTATGATATC CACCGGCAGA GCGTCAGCAA GCTGCAGAAC 
GTGGAGCAAC TCCCGCCCGA TGAGATTAAA GAGCTGTGCC AGTCTGAGAT GCCGGTTGGC 
GTAGATAAAA TCTCGACGAC GCAGAAGTAC GCGCTGGCAC GGCGGCGACT CGTGAAGCTT 
ATCAACAGCA GAGCCAAACT GCTTTCTCTT TGTAGTTATA TCATAGAAAC GTGTCTGTTC 
ATTCTGTGGC GCCACTTGGA ACATTACCTT CTGCACTGCA CCACCAGCGA CTCCCAGGAC 
CCCGTCTTCT CGAATACGAC CTTCGGCAAC AGAAGGTTTC AAGATACAAT TAACACTGAT 
CCTAACATGG ACCTGAGGAA CCTGAGACAG AATAAAGTCA GCCAACAGGA AATTGATATG 
CTGCTAAGAG AAGGCGCCAA TAGTTTCGGG GAGTCCCTGC AGAAGAGACT GTTAGACATC 
GAATCATTGT ACTGCAAGGT GCGATCCCGT CACAGCTTCA TCCAGGCGCT GGTCAGGCGG 
ATCCGGGGCC TCCTGCGAGT ATCAAGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup205 ( XM_012958684.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814138.1 Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X2, mRNA.

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CloneID	OXa53359
Clone ID Related Accession (Same CDS sequence)	XM_012958684.3
Accession Version	XM_012958684.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear pore complex protein Nup205 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC AGTTGGCGCT AAATTCAGAA GCCAGCCTGT GGGGCCCTTA TAGAGAAATA 
TGGCAGACCG TGGTGGGGGC GCTTGTGAAG AGGCAACCGG AAGCCATTCA TTCGCTCGAT 
ATCGTCTTAA AGAAATATAA ACCGGACTTC ATCTCTCTCT TCAAAAATCC ACCAAAGAGC 
GCTCAGCAAC ATGAAAGAGT GCAGAAAGCC AGCAGCGAGG GGATTCCTAT TAAAGGGACG 
CAGCGGACCC GGATCCTGGA AGGACAGCTC ATTAAGGAAG CCTTCATCCT TAGCGACCTG 
TATAATATCG GAGAGGTGGC AGCCGTAGAA CTTCTACTTT TAGGTGAGCA GCAGCAGCCT 
ACCTTCCATG GCCTGACGAG GGGCTTGGTA GCCATACTGT TGTACTGGGA TGGAAAGAGC 
TGCATGGCTG ATTCCTTATT ACACCTCATC CAGGCAAGAA AAGGCAGGAC CTTCACCTTG 
AATCACAGCC CCGAGCTTGT GTCCATGGTA ACTCGTTTCA CCGATGATCT AATGGAGCAA 
GGCTTGACTA ATAAAATCCT CACCTTGATC TCTCAAATTG ACGTGAACAA TGAGTTTGAC 
AAGTTGCTGA AGGATCGGGG CCTGGGCAAC GAGAAGCATC GGAAAGAGGT TTCTGATCTG 
ATAAAGGAAT GTCGGCAGTC GCTCGCACAC AGCCTGTATT CATGGAGTTG CCAGACACCA 
CTAAACAAAG AAGATACACT ACTTTTGATT GGATACTTGG AAAAAGTAAC AGTAGAAGGA 
GATGGCTCAT TGGACAAAGT GAACTTGACC CTACTGATGT CACTTCTCTA TTGCTTGGAT 
GTTGGCTTTT TGGAGCAAGC GACAGATGAT CGAGAAGAAC TGATAAAGCA GACCAGCATG 
TTCATGGACA GACAGTACAT TGCCGCCATC CATAACCGCC TGCACGACAC TCAGCCGTGG 
AAGCTGCCGG GAATGCAGGC TACGGTCAGA CTCGCTTGGG CCCTGGCTCT TCGAGGGATC 
TCTCAGTTCT CAGAAGTCTT AGAATTTTCA GAAGCAGACG AGCCAATGGC AGAGCTAGCG 
ATCAGCGGCA ATGTCTTCCT CTTCCTCACG GAGGCAGTGG TGGGATCCGA AAGCTTCTGT 
GCAGATGAGT TCTTTATACG AAGGATTCAC AAACTCGTCA CCGATTTCCT TACTCTCATG 
CCCATGAAAG TAAAGCAGCT GAGAAATCGT GCAGAAGAGG ATGCCCGACT GATCCATATG 
AGCATGCAGA TGGGAAATGA GCCCCCTGCT TCTCTGCGTA GGGATTTGGA ACATCTGCTC 
TTACTGATCG GTGAGCTCTA CCGGATAGAC CCATTCCACT TGGAACTGGC GTTGGAATAC 
TGGTGCCCCG CAGAGCCTCT CCAGTCGACA TCCTTGATGG GATCCTTTCT TGGTGTGGCC 
CACCAGAGAC CACCTCAACG TCAGGTCGTC TTGTCCAAGT TCGTGAGACA GATGAGTGAC 
CTGCTGCCAG CCACGCTCTA CATACCTTAT CTTAAGATGC TCAGGGGCCT TGCAAGTGGC 
CCTCAGTGCG CCCATTACTG TTTCAGTCTG CTAAAGGCCA ATGGGGGCAG CAGCGAGAGC 
TTGCAAGCTG CAGGGGGTAG CCCAGTGTCC TGGGACCACT TCTTCCACTC TTTGATGCTG 
TACCACGAGC ACTTGCGGAG GGATCTACCA AGCACAGACA GTATACACCT GCGTCACCCT 
CCTCTCCGTG GTATCACCCA GCGAGAGCTC GATGGCCTGA TTGCCTGCTT GCAGCTCACC 
CTTACCATCG TCGATTGGAG TGAAAGTGCC CGCCTTGCTC TGTGTGAGCA TGCCCAGTGG 
ATGCCAGTGG TCGTTATTCT TGGCCTCCTG CAGTGCAGCA TCCCACCACT CCTTAAGGCT 
GAGCTCCTGA AGACACTGGC TGCATTTGGC AAATCCCCAG AGATCGCAGC ATCCCTCTGG 
CAATCCCTCG AATATACACA GATTTTGCAG ACTGTAAGAA CCCCAGGGCT ACGGCAAGGA 
GTCGGGATTG AGGTGGAACT GAATGAGATC GAGTCTCGGT GTGAGGAGTA CCCCCTGACC 
AGAGCATTCT GCCAGCTCAT TAGCACTTTA GTGGAGAGCT CCTTCCCCAC CAATCTGGGA 
GCGGGTTTGA GAGCACCCGG CTTTGAGCCA TACCTCCAGT TTCTACGGGA CACTGTGTTC 
CTTCGGTTCC GCACTAGAGC CTACAGGAGA GGCGCAGAAA AGTGGGAGGT AGCAGAGGCT 
GTACTGGATG TCTTCTACAA GCTTTTGAAG GACTATGAGC CCCAGCCTGA AGACTTTGTG 
GACCAGTATG TGGAACTCCA AGGAGAAGAA AGAGTTGCTT TCAAGCCCCC GGGGTTCAGC 
CTCATGCATC ACCTTCTCAA TGATTCTCCG ATGCTGGAGC TCTGCCTCAG CCTCATGGAA 
GAGGGGGTCA CCCAGCTGGA CACTTACGCC CCTTTTCCAG GCAAGAAACA CCTAGAGAAA 
GCAGTGGCTT ATTGTTTCAT GCTCCTCAAT CAGACTCTGC AGAAGGAGAA TCGCTTCATG 
GATTTGTTGA GGGAAAGTCA CCTGTCGTTG ATTGTGACCC CTCTGGAGCA GCTCCTGCAG 
GGAATTAACC CACGATCCAA GAAAGCTGAT AATGTCATCA ATATTGCTCG ATATCTCTAC 
CATGGGAACA GCAATCCCGA GCTTGCCTTT GAAAGTGCAA AGATCTTATG CAGCATCGCC 
TGTAACTCCA AGATCCAAGA AAAGATTGTA GGAGACTTTA CCCAAGATCA GAACGTGACC 
CAGAAGCTGA TGGTTGGGTT TGTTTCATGC CTGGACAGCG AGGAAGCAGA GGAGTTACTG 
GATTCAGAGA AAGAGGTGGA GGACCAAGCC AAGCAGACGA ACATCCGGTA CATGACAAAG 
ATTCACATCC TCAATCTTCT GATCACCTCC CTGGAAATGA AAGCCCCCAA CCTTGCCATG 
TTCCTTCTCG GCTACGAACT GAAGAAGCCA GTCAGCACGA CCAACCTGCA GGACTCTGGT 
GTCCTGGGCT GCCCGCGGAC CTGCCTTCAC TCCATTTTAG ACATCTTGAG AAAGGGGACT 
GATGGGCGGG CTGGACCCAT AGCCATATGG GATACTCCTC ATCTTGCTGA ACTGTGTTAC 
CAGGTGATCT ACCAGCTGTG CGCTTGTTCA GATACCTCTG GCCCCACCAT GAGATACCTA 
CGGACCAGCC AGGATTTCCT CTTCACTCAG CTTCAGCACC TGCCCTTCTC TGTGGAAGAG 
TATGAGATCT CCGCCATGAA TCAGATGTCC TGGCTGATGA AGACGGCGTC CATAGAACTG 
CGCATCACCT CCCTAAACCG CCAACGTTCC CACACGCAGA GGCTCCTTCA CCTGCTGCTT 
GACGACATGC CCACCAAACC GTATTCAGCA GCTGATGGCG AAGGAGGAAT GGAGGACGAA 
AGCCGCAGCC TCAGTGGATT TCTGCACTTT GACACTACCT CAAAAGTCCG CCGGAAAATC 
CTCCGCATCC TGGACTCCAT ACATTTCAGC AATGAGATCC CGGATCCCCT GCAGTTGGAT 
TTCTTCGATC GCAGCCAGAT AGAGCAGGTT ATTGCTAACT GCGAGCACAA GAACCGCAGA 
GGGCAGACCG TCTGCAACGT CAAGCTTCTC CATCGGGTTC TGGTTGCAGA AGTGAATGCC 
CTTCAGGGAA TGGCTGCTAT TGGTCAGAGG CCGCTGCTTA TGGAGGAAAT CAACACCATC 
CTTCAGTACG TGGTGGAAAG GAACAAGCTG CTGCAGTGCC TCCACGCCAA ACGCCATGCG 
CTGGAATCAT GGCGGCAACT TGTGGAGATC ATACTGACCG CCTGTCCCCA GGACCTCATT 
CCGACTGAGC ACAGACAGCT CATCATACGA GACCTGCTGC AAGATCTGCA TGATAAGATC 
CTCGACGACG ACGCTGCCCA AGAACTAATG CCCATTGTCG CCGGGGCAGT CTTCACCCTC 
ACCACTCATC TCAGCCAATC TGTACGTACC GAGCTGAAAC AGCCGATGGC TGCCGCTGGG 
CTGGGCCAGT CGCAATATGT GCAAATGCTG GACGGCTCAT TCGCTGCCCC TCCTGGGACA 
GAAAACCTCT CCGCTGGTTT TGCATCCATT GGAGACTCCT CCCTTCACAT GATTCTCAGG 
AATCTGCTCG AGTTCATTCT AAAAACAGGA GGTGGGTTCC AACGGGTCAG GGCCCACCTG 
TACGGATCGC TGCTGTATTA CCTGCAGATA GCTCAGAGAC CAGATGAACC AGATACTCTG 
GAATCCGCAC ACAAGACTAT GTGGGAGCGA CTGACGGCCC CGGAGGATGT ATTCAGCAAG 
CTGCAGCGAG ACAACCTGTC CATATTCGAG AGCTACGGCA CCGCCCTTAT GGAAGTGGTT 
TGCCGGGACG CCTGTGACGG ACATGACATT GGGAGGATGC TGGCCCTGGC ACTGCTTGAC 
CGGATTGTCT CTGTGGATAG GCAACAGTGG CTGCTTTATC TCTCCAACAG TGGCTACCTG 
AAGGTCCTTG TGGATAGCCT GGCAGAGGAC GATATGGTCC TAAGGAACCT GCTAACTCCT 
CAGGCGCCTC TTCTAAAGGC TCTCTATATC TATGAGTCGA AAATGGCATT TCTGACCCGG 
GTGGCTAAAA GCCAGCAGGG AGCCCTGGAG CTGCTGAGGA GTGGGGTTAT CGTCAGGCTG 
GCACAGTGCC AAGTATATGA TATGCGCCCG GAGACTGACC CCCATGGAGT GTTTGGCATG 
AGAGAGACCC CGGTGTTTAT CCCCGCCCCG GTGGAACGAT ACCGGCAAAT CCTGTTACCC 
GCTCTCCAGA TCTGTCAGCT CATCCTGACC TCCAGCACGG CACAGCACTT ACAGGCCGCC 
GGACAGGTCT TGCAGTTCCT GATCGCTCAT TCTGATACCA TCCAGGCAAT TCTCCGGAGC 
CAGGAGGGGA GTCTGGGTTC CTTGCAGGAG TTGGCTTTGC TTACAGGCAT CATCAGCAAG 
GCCGCACTAC CCGGTGTTCT CAACGAGCTC GATATCGGTT TAAACGAAGG GTCCATGATG 
GAGTTACAAG GACATATTGG CCGCTTCCAG CGCCAGTGTC TTGCGTTATT AAACCGCTTT 
GGGGGGTCCG ACCGACTTCG GCAGCTCAGT CTGCAGGATG ATTCCTCCCA GCCGGACGGT 
GTGAGCAAGA GGGACGACAT GGAGTTGGCC ATGCAACAGA TCTGCTCCAA CGTGATGGAG 
TACTGCCAGA CGCTTATGAT ACAGAACTCG CCAAGCTTCC AGCAGACTGT GTGTCTCTTC 
ACCCCCAACC TCACGGAGAG CTCCAGCCGA GATGGAACAC GGCAGGATTC CCAGGTGTCC 
ATTCTGCCGT CATGGCGGTT GCCAGGCTTG GGTATAGTGA TCCACCTTCT GAAGCAGAGC 
GCCAATAACT TCTTCACTTA CTATGATATC CACCGGCAGA GCGTCAGCAA GCTGCAGAAC 
GTGGAGCAAC TCCCGCCCGA TGAGATTAAA GAGCTGTGCC AGTCTGAGAT GCCGGTTGGC 
GTAGATAAAA TCTCGACGAC GCAGAAGTAC GCGCTGGCAC GGCGGCGACT CGTGAAGCTT 
ATCAACAGCA GAGCCAAACT GCTTTCTCTT TGTAGTTATA TCATAGAAAC GTGTCTGTTC 
ATTCTGTGGC GCCACTTGGA ACATTACCTT CTGCACTGCA CCACCAGCGA CTCCCAGGAC 
CCCGTCTTCT CGAATACGAC CTTCGGCAAC AGAAGGTTTC AAGATACAAT TAACACTGAT 
CCTAACATGG ACCTGAGGAA CCTGAGACAG AATAAAGTCA GCCAACAGGA AATTGATATG 
CTGCTAAGAG AAGGCGCCAA TAGTTTCGGG GAGTCCCTGC AGAAGAGACT GTTAGACATC 
GAATCATTGT ACTGCAAGGT GCGATCCCGT CACAGCTTCA TCCAGGCGCT GGTCAGGCGG 
ATCCGGGGCC TCCTGCGAGT ATCAAGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814138.1
CDS	238..6270
Translation	MAAQLALNSEASLWGPYREIWQTVVGALVKRQPEAIHSLDIVLKKYKPDFISLFKNPPKSAQQHERVQKASSEGIPIKGTQRTRILEGQLIKEAFILSDLYNIGEVAAVELLLLGEQQQPTFHGLTRGLVAILLYWDGKSCMADSLLHLIQARKGRTFTLNHSPELVSMVTRFTDDLMEQGLTNKILTLISQIDVNNEFDKLLKDRGLGNEKHRKEVSDLIKECRQSLAHSLYSWSCQTPLNKEDTLLLIGYLEKVTVEGDGSLDKVNLTLLMSLLYCLDVGFLEQATDDREELIKQTSMFMDRQYIAAIHNRLHDTQPWKLPGMQATVRLAWALALRGISQFSEVLEFSEADEPMAELAISGNVFLFLTEAVVGSESFCADEFFIRRIHKLVTDFLTLMPMKVKQLRNRAEEDARLIHMSMQMGNEPPASLRRDLEHLLLLIGELYRIDPFHLELALEYWCPAEPLQSTSLMGSFLGVAHQRPPQRQVVLSKFVRQMSDLLPATLYIPYLKMLRGLASGPQCAHYCFSLLKANGGSSESLQAAGGSPVSWDHFFHSLMLYHEHLRRDLPSTDSIHLRHPPLRGITQRELDGLIACLQLTLTIVDWSESARLALCEHAQWMPVVVILGLLQCSIPPLLKAELLKTLAAFGKSPEIAASLWQSLEYTQILQTVRTPGLRQGVGIEVELNEIESRCEEYPLTRAFCQLISTLVESSFPTNLGAGLRAPGFEPYLQFLRDTVFLRFRTRAYRRGAEKWEVAEAVLDVFYKLLKDYEPQPEDFVDQYVELQGEERVAFKPPGFSLMHHLLNDSPMLELCLSLMEEGVTQLDTYAPFPGKKHLEKAVAYCFMLLNQTLQKENRFMDLLRESHLSLIVTPLEQLLQGINPRSKKADNVINIARYLYHGNSNPELAFESAKILCSIACNSKIQEKIVGDFTQDQNVTQKLMVGFVSCLDSEEAEELLDSEKEVEDQAKQTNIRYMTKIHILNLLITSLEMKAPNLAMFLLGYELKKPVSTTNLQDSGVLGCPRTCLHSILDILRKGTDGRAGPIAIWDTPHLAELCYQVIYQLCACSDTSGPTMRYLRTSQDFLFTQLQHLPFSVEEYEISAMNQMSWLMKTASIELRITSLNRQRSHTQRLLHLLLDDMPTKPYSAADGEGGMEDESRSLSGFLHFDTTSKVRRKILRILDSIHFSNEIPDPLQLDFFDRSQIEQVIANCEHKNRRGQTVCNVKLLHRVLVAEVNALQGMAAIGQRPLLMEEINTILQYVVERNKLLQCLHAKRHALESWRQLVEIILTACPQDLIPTEHRQLIIRDLLQDLHDKILDDDAAQELMPIVAGAVFTLTTHLSQSVRTELKQPMAAAGLGQSQYVQMLDGSFAAPPGTENLSAGFASIGDSSLHMILRNLLEFILKTGGGFQRVRAHLYGSLLYYLQIAQRPDEPDTLESAHKTMWERLTAPEDVFSKLQRDNLSIFESYGTALMEVVCRDACDGHDIGRMLALALLDRIVSVDRQQWLLYLSNSGYLKVLVDSLAEDDMVLRNLLTPQAPLLKALYIYESKMAFLTRVAKSQQGALELLRSGVIVRLAQCQVYDMRPETDPHGVFGMRETPVFIPAPVERYRQILLPALQICQLILTSSTAQHLQAAGQVLQFLIAHSDTIQAILRSQEGSLGSLQELALLTGIISKAALPGVLNELDIGLNEGSMMELQGHIGRFQRQCLALLNRFGGSDRLRQLSLQDDSSQPDGVSKRDDMELAMQQICSNVMEYCQTLMIQNSPSFQQTVCLFTPNLTESSSRDGTRQDSQVSILPSWRLPGLGIVIHLLKQSANNFFTYYDIHRQSVSKLQNVEQLPPDEIKELCQSEMPVGVDKISTTQKYALARRRLVKLINSRAKLLSLCSYIIETCLFILWRHLEHYLLHCTTSDSQDPVFSNTTFGNRRFQDTINTDPNMDLRNLRQNKVSQQEIDMLLREGANSFGESLQKRLLDIESLYCKVRSRHSFIQALVRRIRGLLRVSRA

Target ORF information:

RefSeq Version	XM_012958684.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958684.3

ORF Insert Sequence:

ATGGCGGCGC AGTTGGCGCT AAATTCAGAA GCCAGCCTGT GGGGCCCTTA TAGAGAAATA 
TGGCAGACCG TGGTGGGGGC GCTTGTGAAG AGGCAACCGG AAGCCATTCA TTCGCTCGAT 
ATCGTCTTAA AGAAATATAA ACCGGACTTC ATCTCTCTCT TCAAAAATCC ACCAAAGAGC 
GCTCAGCAAC ATGAAAGAGT GCAGAAAGCC AGCAGCGAGG GGATTCCTAT TAAAGGGACG 
CAGCGGACCC GGATCCTGGA AGGACAGCTC ATTAAGGAAG CCTTCATCCT TAGCGACCTG 
TATAATATCG GAGAGGTGGC AGCCGTAGAA CTTCTACTTT TAGGTGAGCA GCAGCAGCCT 
ACCTTCCATG GCCTGACGAG GGGCTTGGTA GCCATACTGT TGTACTGGGA TGGAAAGAGC 
TGCATGGCTG ATTCCTTATT ACACCTCATC CAGGCAAGAA AAGGCAGGAC CTTCACCTTG 
AATCACAGCC CCGAGCTTGT GTCCATGGTA ACTCGTTTCA CCGATGATCT AATGGAGCAA 
GGCTTGACTA ATAAAATCCT CACCTTGATC TCTCAAATTG ACGTGAACAA TGAGTTTGAC 
AAGTTGCTGA AGGATCGGGG CCTGGGCAAC GAGAAGCATC GGAAAGAGGT TTCTGATCTG 
ATAAAGGAAT GTCGGCAGTC GCTCGCACAC AGCCTGTATT CATGGAGTTG CCAGACACCA 
CTAAACAAAG AAGATACACT ACTTTTGATT GGATACTTGG AAAAAGTAAC AGTAGAAGGA 
GATGGCTCAT TGGACAAAGT GAACTTGACC CTACTGATGT CACTTCTCTA TTGCTTGGAT 
GTTGGCTTTT TGGAGCAAGC GACAGATGAT CGAGAAGAAC TGATAAAGCA GACCAGCATG 
TTCATGGACA GACAGTACAT TGCCGCCATC CATAACCGCC TGCACGACAC TCAGCCGTGG 
AAGCTGCCGG GAATGCAGGC TACGGTCAGA CTCGCTTGGG CCCTGGCTCT TCGAGGGATC 
TCTCAGTTCT CAGAAGTCTT AGAATTTTCA GAAGCAGACG AGCCAATGGC AGAGCTAGCG 
ATCAGCGGCA ATGTCTTCCT CTTCCTCACG GAGGCAGTGG TGGGATCCGA AAGCTTCTGT 
GCAGATGAGT TCTTTATACG AAGGATTCAC AAACTCGTCA CCGATTTCCT TACTCTCATG 
CCCATGAAAG TAAAGCAGCT GAGAAATCGT GCAGAAGAGG ATGCCCGACT GATCCATATG 
AGCATGCAGA TGGGAAATGA GCCCCCTGCT TCTCTGCGTA GGGATTTGGA ACATCTGCTC 
TTACTGATCG GTGAGCTCTA CCGGATAGAC CCATTCCACT TGGAACTGGC GTTGGAATAC 
TGGTGCCCCG CAGAGCCTCT CCAGTCGACA TCCTTGATGG GATCCTTTCT TGGTGTGGCC 
CACCAGAGAC CACCTCAACG TCAGGTCGTC TTGTCCAAGT TCGTGAGACA GATGAGTGAC 
CTGCTGCCAG CCACGCTCTA CATACCTTAT CTTAAGATGC TCAGGGGCCT TGCAAGTGGC 
CCTCAGTGCG CCCATTACTG TTTCAGTCTG CTAAAGGCCA ATGGGGGCAG CAGCGAGAGC 
TTGCAAGCTG CAGGGGGTAG CCCAGTGTCC TGGGACCACT TCTTCCACTC TTTGATGCTG 
TACCACGAGC ACTTGCGGAG GGATCTACCA AGCACAGACA GTATACACCT GCGTCACCCT 
CCTCTCCGTG GTATCACCCA GCGAGAGCTC GATGGCCTGA TTGCCTGCTT GCAGCTCACC 
CTTACCATCG TCGATTGGAG TGAAAGTGCC CGCCTTGCTC TGTGTGAGCA TGCCCAGTGG 
ATGCCAGTGG TCGTTATTCT TGGCCTCCTG CAGTGCAGCA TCCCACCACT CCTTAAGGCT 
GAGCTCCTGA AGACACTGGC TGCATTTGGC AAATCCCCAG AGATCGCAGC ATCCCTCTGG 
CAATCCCTCG AATATACACA GATTTTGCAG ACTGTAAGAA CCCCAGGGCT ACGGCAAGGA 
GTCGGGATTG AGGTGGAACT GAATGAGATC GAGTCTCGGT GTGAGGAGTA CCCCCTGACC 
AGAGCATTCT GCCAGCTCAT TAGCACTTTA GTGGAGAGCT CCTTCCCCAC CAATCTGGGA 
GCGGGTTTGA GAGCACCCGG CTTTGAGCCA TACCTCCAGT TTCTACGGGA CACTGTGTTC 
CTTCGGTTCC GCACTAGAGC CTACAGGAGA GGCGCAGAAA AGTGGGAGGT AGCAGAGGCT 
GTACTGGATG TCTTCTACAA GCTTTTGAAG GACTATGAGC CCCAGCCTGA AGACTTTGTG 
GACCAGTATG TGGAACTCCA AGGAGAAGAA AGAGTTGCTT TCAAGCCCCC GGGGTTCAGC 
CTCATGCATC ACCTTCTCAA TGATTCTCCG ATGCTGGAGC TCTGCCTCAG CCTCATGGAA 
GAGGGGGTCA CCCAGCTGGA CACTTACGCC CCTTTTCCAG GCAAGAAACA CCTAGAGAAA 
GCAGTGGCTT ATTGTTTCAT GCTCCTCAAT CAGACTCTGC AGAAGGAGAA TCGCTTCATG 
GATTTGTTGA GGGAAAGTCA CCTGTCGTTG ATTGTGACCC CTCTGGAGCA GCTCCTGCAG 
GGAATTAACC CACGATCCAA GAAAGCTGAT AATGTCATCA ATATTGCTCG ATATCTCTAC 
CATGGGAACA GCAATCCCGA GCTTGCCTTT GAAAGTGCAA AGATCTTATG CAGCATCGCC 
TGTAACTCCA AGATCCAAGA AAAGATTGTA GGAGACTTTA CCCAAGATCA GAACGTGACC 
CAGAAGCTGA TGGTTGGGTT TGTTTCATGC CTGGACAGCG AGGAAGCAGA GGAGTTACTG 
GATTCAGAGA AAGAGGTGGA GGACCAAGCC AAGCAGACGA ACATCCGGTA CATGACAAAG 
ATTCACATCC TCAATCTTCT GATCACCTCC CTGGAAATGA AAGCCCCCAA CCTTGCCATG 
TTCCTTCTCG GCTACGAACT GAAGAAGCCA GTCAGCACGA CCAACCTGCA GGACTCTGGT 
GTCCTGGGCT GCCCGCGGAC CTGCCTTCAC TCCATTTTAG ACATCTTGAG AAAGGGGACT 
GATGGGCGGG CTGGACCCAT AGCCATATGG GATACTCCTC ATCTTGCTGA ACTGTGTTAC 
CAGGTGATCT ACCAGCTGTG CGCTTGTTCA GATACCTCTG GCCCCACCAT GAGATACCTA 
CGGACCAGCC AGGATTTCCT CTTCACTCAG CTTCAGCACC TGCCCTTCTC TGTGGAAGAG 
TATGAGATCT CCGCCATGAA TCAGATGTCC TGGCTGATGA AGACGGCGTC CATAGAACTG 
CGCATCACCT CCCTAAACCG CCAACGTTCC CACACGCAGA GGCTCCTTCA CCTGCTGCTT 
GACGACATGC CCACCAAACC GTATTCAGCA GCTGATGGCG AAGGAGGAAT GGAGGACGAA 
AGCCGCAGCC TCAGTGGATT TCTGCACTTT GACACTACCT CAAAAGTCCG CCGGAAAATC 
CTCCGCATCC TGGACTCCAT ACATTTCAGC AATGAGATCC CGGATCCCCT GCAGTTGGAT 
TTCTTCGATC GCAGCCAGAT AGAGCAGGTT ATTGCTAACT GCGAGCACAA GAACCGCAGA 
GGGCAGACCG TCTGCAACGT CAAGCTTCTC CATCGGGTTC TGGTTGCAGA AGTGAATGCC 
CTTCAGGGAA TGGCTGCTAT TGGTCAGAGG CCGCTGCTTA TGGAGGAAAT CAACACCATC 
CTTCAGTACG TGGTGGAAAG GAACAAGCTG CTGCAGTGCC TCCACGCCAA ACGCCATGCG 
CTGGAATCAT GGCGGCAACT TGTGGAGATC ATACTGACCG CCTGTCCCCA GGACCTCATT 
CCGACTGAGC ACAGACAGCT CATCATACGA GACCTGCTGC AAGATCTGCA TGATAAGATC 
CTCGACGACG ACGCTGCCCA AGAACTAATG CCCATTGTCG CCGGGGCAGT CTTCACCCTC 
ACCACTCATC TCAGCCAATC TGTACGTACC GAGCTGAAAC AGCCGATGGC TGCCGCTGGG 
CTGGGCCAGT CGCAATATGT GCAAATGCTG GACGGCTCAT TCGCTGCCCC TCCTGGGACA 
GAAAACCTCT CCGCTGGTTT TGCATCCATT GGAGACTCCT CCCTTCACAT GATTCTCAGG 
AATCTGCTCG AGTTCATTCT AAAAACAGGA GGTGGGTTCC AACGGGTCAG GGCCCACCTG 
TACGGATCGC TGCTGTATTA CCTGCAGATA GCTCAGAGAC CAGATGAACC AGATACTCTG 
GAATCCGCAC ACAAGACTAT GTGGGAGCGA CTGACGGCCC CGGAGGATGT ATTCAGCAAG 
CTGCAGCGAG ACAACCTGTC CATATTCGAG AGCTACGGCA CCGCCCTTAT GGAAGTGGTT 
TGCCGGGACG CCTGTGACGG ACATGACATT GGGAGGATGC TGGCCCTGGC ACTGCTTGAC 
CGGATTGTCT CTGTGGATAG GCAACAGTGG CTGCTTTATC TCTCCAACAG TGGCTACCTG 
AAGGTCCTTG TGGATAGCCT GGCAGAGGAC GATATGGTCC TAAGGAACCT GCTAACTCCT 
CAGGCGCCTC TTCTAAAGGC TCTCTATATC TATGAGTCGA AAATGGCATT TCTGACCCGG 
GTGGCTAAAA GCCAGCAGGG AGCCCTGGAG CTGCTGAGGA GTGGGGTTAT CGTCAGGCTG 
GCACAGTGCC AAGTATATGA TATGCGCCCG GAGACTGACC CCCATGGAGT GTTTGGCATG 
AGAGAGACCC CGGTGTTTAT CCCCGCCCCG GTGGAACGAT ACCGGCAAAT CCTGTTACCC 
GCTCTCCAGA TCTGTCAGCT CATCCTGACC TCCAGCACGG CACAGCACTT ACAGGCCGCC 
GGACAGGTCT TGCAGTTCCT GATCGCTCAT TCTGATACCA TCCAGGCAAT TCTCCGGAGC 
CAGGAGGGGA GTCTGGGTTC CTTGCAGGAG TTGGCTTTGC TTACAGGCAT CATCAGCAAG 
GCCGCACTAC CCGGTGTTCT CAACGAGCTC GATATCGGTT TAAACGAAGG GTCCATGATG 
GAGTTACAAG GACATATTGG CCGCTTCCAG CGCCAGTGTC TTGCGTTATT AAACCGCTTT 
GGGGGGTCCG ACCGACTTCG GCAGCTCAGT CTGCAGGATG ATTCCTCCCA GCCGGACGGT 
GTGAGCAAGA GGGACGACAT GGAGTTGGCC ATGCAACAGA TCTGCTCCAA CGTGATGGAG 
TACTGCCAGA CGCTTATGAT ACAGAACTCG CCAAGCTTCC AGCAGACTGT GTGTCTCTTC 
ACCCCCAACC TCACGGAGAG CTCCAGCCGA GATGGAACAC GGCAGGATTC CCAGGTGTCC 
ATTCTGCCGT CATGGCGGTT GCCAGGCTTG GGTATAGTGA TCCACCTTCT GAAGCAGAGC 
GCCAATAACT TCTTCACTTA CTATGATATC CACCGGCAGA GCGTCAGCAA GCTGCAGAAC 
GTGGAGCAAC TCCCGCCCGA TGAGATTAAA GAGCTGTGCC AGTCTGAGAT GCCGGTTGGC 
GTAGATAAAA TCTCGACGAC GCAGAAGTAC GCGCTGGCAC GGCGGCGACT CGTGAAGCTT 
ATCAACAGCA GAGCCAAACT GCTTTCTCTT TGTAGTTATA TCATAGAAAC GTGTCTGTTC 
ATTCTGTGGC GCCACTTGGA ACATTACCTT CTGCACTGCA CCACCAGCGA CTCCCAGGAC 
CCCGTCTTCT CGAATACGAC CTTCGGCAAC AGAAGGTTTC AAGATACAAT TAACACTGAT 
CCTAACATGG ACCTGAGGAA CCTGAGACAG AATAAAGTCA GCCAACAGGA AATTGATATG 
CTGCTAAGAG AAGGCGCCAA TAGTTTCGGG GAGTCCCTGC AGAAGAGACT GTTAGACATC 
GAATCATTGT ACTGCAAGGT GCGATCCCGT CACAGCTTCA TCCAGGCGCT GGTCAGGCGG 
ATCCGGGGCC TCCTGCGAGT ATCAAGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup205 ( NM_001142124.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001135596.1 Xenopus tropicalis nucleoporin 205kDa (nup205), mRNA.

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CloneID	OXa21731
Clone ID Related Accession (Same CDS sequence)	NM_001142124.1
Accession Version	NM_001142124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear pore complex protein Nup205
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168124.1. ##Evidence-Data-START## Transcript exon combination :: BC168124.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGC AGTTGGCGCT AAATTCAGAA GCCAGCCTGT GGGGCCCTTA TAGAGAAATA 
TGGCAGACCG TGGTGGGGGC GCTTGTGAAG AGGCAACCGG AAGCCATTCA TTCGCTCGAT 
ATCGTCTTAA AGAAATATAA ACCGGACTTC ATCTCTCTCT TCAAAAATCC ACCAAAGAGC 
GCTCAGCAAC ATGAAAGAGT GCAGAAAGCC AGCAGCGAGG GGATTCCTAT TAAAGGGACG 
CAGCGGACCC GGATCCTGGA AGGACAGCTC ATTAAGGAAG CCTTCATCCT TAGCGACCTG 
TATAATATCG GAGAGGTGGC AGCCGTAGAA CTTCTACTTT TAGGTGAGCA GCAGCAGCCT 
ACCTTCCATG GCCTGACGAG GGGCTTGGTA GCCATACTGT TGTACTGGGA TGGAAAGAGC 
TGCATGGCTG ATTCCTTATT ACACCTCATC CAGGCAAGAA AAGGCAGGAC CTTCACCTTG 
AATCACAGCC CCGAGCTTGT GTCCATGGTA ACTCGTTTCA CCGATGATCT AATGGAGCAA 
GGCTTGACTA ATAAAATCCT CACCTTGATC TCTCAAATTG ACGTGAACAA TGAGTTTGAC 
AAGTTGCTGA AGGATCGGGG CCTGGGCAAC GAGAAGCATC GGAAAGAGGT TTCTGATCTG 
ATAAAGGAAT GTCGGCAGTC GCTCGCACAC AGCCTGTATT CATGGAGTTG CCAGACACCA 
CTAAACAAAG AAGATACACT ACTTTTGATT GGATACTTGG AAAAAGTAAC AGTAGAAGGA 
GATGGCTCAT TGGACAAAGT GAACTTGACC CTACTGATGT CACTTCTCTA TTGCTTGGAT 
GTTGGCTTTT TGGAGCAAGC GACAGATGAT CGAGAAGAAC TGATAAAGCA GACCAGCATG 
TTCATGGACA GACAGTACAT TGCCGCCATC CATAACCGCC TGCACGACAC TCAGCCGTGG 
AAGCTGCCGG GAATGCAGGC TACGGTCAGA CTCGCTTGGG CCCTGGCTCT TCGAGGGATC 
TCTCAGTTCT CAGAAGTCTT AGAATTTTCA GAAGCAGACG AGCCAATGGC AGAGCTAGCG 
ATCAGCGGCA ATGTCTTCCT CTTCCTCACG GAGGCAGTGG TGGGATCCGA AAGCTTCTGT 
GCAGATGAGT TCTTTATACG AAGGATTCAC AAACTCGTCA CCGATTTCCT TACTCTCATG 
CCCATGAAAG TAAAGCAGCT GAGAAATCGT GCAGAAGAGG ATGCCCGACT GATCCATATG 
AGCATGCAGA TGGGAAATGA GCCCCCTGCT TCTCTGCGTA GGGATTTGGA ACATCTGCTC 
TTACTGATCG GTGAGCTCTA CCGGATAGAC CCATTCCACT TGGAACTGGC GTTGGAATAC 
TGGTGCCCCG CAGAGCCTCT CCAGTCGACA TCCTTGATGG GATCCTTTCT TGGTGTGGCC 
CACCAGAGAC CACCTCAACG TCAGGTCGTC TTGTCCAAGT TCGTGAGACA GATGAGTGAC 
CTGCTGCCAG CCACGCTCTA CATACCTTAT CTTAAGATGC TCAGGGGCCT TGCAAGTGGC 
CCTCAGTGCG CCCATTACTG TTTCAGTCTG CTAAAGGCCA ATGGGGGCAG CAGCGCAGAG 
AGCTTGCAAG CTGCAGGGGG TAGCCCAGTG TCCTGGGACC ACTTCTTCCA CTCTTTGATG 
CTGTACCACG AGCACTTGCG GAGGGATCTA CCAAGCACAG ACAGTATACA CCTGCGTCAC 
CCTCCTCTCC GTGGTATCAC CCAGCGAGAG CTCGATGGCC TGATTGCCTG CTTGCAGCTC 
ACCCTTACCA TCGTCGATTG GAGTGAAAGT GCCCGCCTTG CTCTGTGTGA GCATGCCCAG 
TGGATGCCAG TGGTCGTTAT TCTTGGCCTC CTGCAGTGCA GCATCCCACC ACTCCTTAAG 
GCTGAGCTCC TGAAGACACT GGCTGCATTT GGCAAATCCC CAGAGATCGC AGCATCCCTC 
TGGCAATCCC TCGAATATAC ACAGATTTTG CAGACTGTAA GAACCCCAGG GCTACGGCAA 
GGAGTCGGGA TTGAGGTGGA ACTGAATGAG ATCGAGTCTC GGTGTGAGGA GTACCCCCTG 
ACCAGAGCAT TCTGCCAGCT CATTAGCACT TTAGTGGAGA GCTCCTTCCC CACCAATCTG 
GGAGCGGGTT TGAGAGCACC CGGCTTTGAG CCATACCTCC AGTTTCTACG GGACACTGTG 
TTCCTTCGGT TCCGCACTAG AGCCTACAGG AGAGGCGCAG AAAAGTGGGA GGTAGCAGAG 
GCTGTACTGG ATGTCTTCTA CAAGCTTTTG AAGGACTATG AGCCCCAGCC TGAAGACTTT 
GTGGACCAGT ATGTGGAACT CCAAGGAGAA GAAAGAGTTG CTTTCAAGCC CCCGGGGTTC 
AGCCTCATGC ATCACCTTCT CAATGATTCT CCGATGCTGG AGCTCTGCCT CAGCCTCATG 
GAAGAGGGGG TCACCCAGCT GGACACTTAC GCCCCTTTTC CAGGCAAGAA ACACCTAGAG 
AAAGCAGTGG CTTATTGTTT CATGCTCCTC AATCAGACTC TGCAGAAGGA GAATCGCTTC 
ATGGATTTGT TGAGGGAAAG TCACCTGTCG TTGATTGTGA CCCCTCTGGA GCAGCTCCTG 
CAGGGAATTA ACCCACGATC CAAGAAAGCT GATAATGTCA TCAATATTGC TCGATATCTC 
TACCATGGGA ACAGCAATCC CGAGCTTGCC TTTGAAAGTG CAAAGATCTT ATGCAGCATC 
GCCTGTAACT CCAAGATCCA AGAAAAGATT GTAGGAGACT TTACCCAAGA TCAGAACGTG 
ACCCAGAAGC TGATGGTTGG GTTTGTTTCA TGCCTGGACA GCGAGGAAGC AGAGGAGTTA 
CTGGATTCAG AGAAAGAGGT GGAGGACCAA GCCAAGCAGA CGAACATCCG GTACATGACA 
AAGATTCACA TCCTCAATCT TCTGATCACC TCCCTGGAAA TGAAAGCCCC CAACCTTGCC 
ATGTTCCTTC TCGGCTACGA ACTGAAGAAG CCAGTCAGCA CGACCAACCT GCAGGACTCT 
GGTGTCCTGG GCTGCCCGCG GACCTGCCTT CACTCCATTT TAGACATCTT GAGAAAGGGG 
ACTGATGGGC GGGCTGGACC CATAGCCATA TGGGATACTC CTCATCTTGC TGAACTGTGT 
TACCAGGTGA TCTACCAGCT GTGCGCTTGT TCAGATACCT CTGGCCCCAC CATGAGATAC 
CTACGGACCA GCCAGGATTT CCTCTTCACT CAGCTTCAGC ACCTGCCCTT CTCTGTGGAA 
GAGTATGAGA TCTCCGCCAT GAATCAGATG TCCTGGCTGA TGAAGACGGC GTCCATAGAA 
CTGCGCATCA CCTCCCTAAA CCGCCAACGT TCCCACACGC AGAGGCTCCT TCACCTGCTG 
CTTGACGACA TGCCCACCAA ACCGTATTCA GCAGCTGATG GCGAAGGAGG AATGGAGGAC 
GAAAGCCGCA GCCTCAGTGG ATTTCTGCAC TTTGACACTA CCTCAAAAGT CCGCCGGAAA 
ATCCTCCGCA TCCTGGACTC CATACATTTC AGCAATGAGA TCCCGGATCC CCTGCAGTTG 
GATTTCTTCG ATCGCAGCCA GATAGAGCAG GTTATTGCTA ACTGCGAGCA CAAGAACCGC 
AGAGGGCAGA CCGTCTGCAA CGTCAAGCTT CTCCATCGGG TTCTGGTTGC AGAAGTGAAT 
GCCCTTCAGG GAATGGCTGC TATTGGTCAG AGGCCGCTGC TTATGGAGGA AATCAACACC 
ATCCTTCAGT ACGTGGTGGA AAGGAACAAG CTGCTGCAGT GCCTCCACGC CAAACGCCAT 
GCGCTGGAAT CATGGCGGCA ACTTGTGGAG ATCATACTGA CCGCCTGTCC CCAGGACCTC 
ATTCCGACTG AGCACAGACA GCTCATCATA CGAGACCTGC TGCAAGATCT GCATGATAAG 
ATCCTCGACG ACGACGCTGC CCAAGAACTA ATGCCCATTG TCGCCGGGGC AGTCTTCACC 
CTCACCACTC ATCTCAGCCA ATCTGTACGT ACCGAGCTGA AACAGCCGAT GGCTGCCGCT 
GGGCTGGGCC AGTCGCAATA TGTGCAAATG CTGGACGGCT CATTCGCTGC CCCTCCTGGG 
ACAGAAAACC TCTCCGCTGG TTTTGCATCC ATTGGAGACT CCTCCCTTCA CATGATTCTC 
AGGAATCTGC TCGAGTTCAT TCTAAAAACA GGAGGTGGGT TCCAACGGGT CAGGGCCCAC 
CTGTACGGAT CGCTGCTGTA TTACCTGCAG ATAGCTCAGA GACCAGATGA ACCAGATACT 
CTGGAATCCG CACACAAGAC TATGTGGGAG CGACTGACGG CCCCGGAGGA TGTATTCAGC 
AAGCTGCAGC GAGACAACCT GTCCATATTC GAGAGCTACG GCACCGCCCT TATGGAAGTG 
GTTTGCCGGG ACGCCTGTGA CGGACATGAC ATTGGGAGGA TGCTGGCCCT GGCACTGCTT 
GACCGGATTG TCTCTGTGGA TAGGCAACAG TGGCTGCTTT ATCTCTCCAA CAGTGGCTAC 
CTGAAGGTCC TTGTGGATAG CCTGGCAGAG GACGATATGG TCCTAAGGAA CCTGCTAACT 
CCTCAGGCGC CTCTTCTAAA GGCTCTCTAT ATCTATGAGT CGAAAATGGC ATTTCTGACC 
CGGGTGGCTA AAAGCCAGCA GGGAGCCCTG GAGCTGCTGA GGAGTGGGGT TATCGTCAGG 
CTGGCACAGT GCCAAGTATA TGATATGCGC CCGGAGACTG ACCCCCATGG AGTGTTTGGC 
ATGAGAGAGA CCCCGGTGTT TATCCCCGCC CCGGTGGAAC GATACCGGCA AATCCTGTTA 
CCCGCTCTCC AGATCTGTCA GCTCATCCTG ACCTCCAGCA CGGCACAGCA CTTACAGGCC 
GCCGGACAGG TCTTGCAGTT CCTGATCGCT CATTCTGATA CCATCCAGGC AATTCTCCGG 
AGCCAGGAGG GGAGTCTGGG TTCCTTGCAG GAGTTGGCTT TGCTTACAGG CATCATCAGC 
AAGGCCGCAC TACCCGGTGT TCTCAACGAG CTCGATATCG GTTTAAACGA AGGGTCCATG 
ATGGAGTTAC AAGGACATAT TGGCCGCTTC CAGCGCCAGT GTCTTGCGTT ATTAAACCGC 
TTTGGGGGGT CCGACCGACT TCGGCAGCTC AGTCTGCAGG ATGATTCCTC CCAGCCGGAC 
GGTGTGAGCA AGAGGGACGA CATGGAGTTG GCCATGCAAC AGATCTGCTC CAACGTGATG 
GAGTACTGCC AGACGCTTAT GATACAGAAC TCGCCAAGCT TCCAGCAGAC TGTGTGTCTC 
TTCACCCCCA ACCTCACGGA GAGCTCCAGC CGAGATGGAA CACGGCAGGA TTCCCAGGTG 
TCCATTCTGC CGTCATGGCG GTTGCCAGGC TTGGGTATAG TGATCCACCT TCTGAAGCAG 
AGCGCCAATA ACTTCTTCAC TTACTATGAT ATCCACCGGC AGAGCGTCAG CAAGCTGCAG 
AACGTGGAGC AACTCCCGCC CGATGAGATT AAAGAGCTGT GCCAGTCTGA GATGCCGGTT 
GGCGTAGATA AAATCTCGAC GACGCAGAAG TACGCGCTGG CACGGCGGCG ACTCGTGAAG 
CTTATCAACA GCAGAGCCAA ACTGCTTTCT CTTTGTAGTT ATATCATAGA AACGTGTCTG 
TTCATTCTGT GGCGCCACTT GGAACATTAC CTTCTGCACT GCACCACCAG CGACTCCCAG 
GACCCCGTCT TCTCGAATAC GACCTTCGGC AACAGAAGGT TTCAAGATAC AATTAACACT 
GATCCTAACA TGGACCTGAG GAACCTGAGA CAGAATAAAG TCAGCCAACA GGAAATTGAT 
ATGCTGCTAA GAGAAGGCGC CAATAGTTTC GGGGAGTCCC TGCAGAAGAG ACTGTTAGAC 
ATCGAATCAT TGTACTGCAA GGTGCGATCC CGTCACAGCT TCATCCAGGC GCTGGTCAGG 
CGGATCCGGG GCCTCCTGCG AGTATCAAGG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135596.1
CDS	6..6041
Translation	MAAQLALNSEASLWGPYREIWQTVVGALVKRQPEAIHSLDIVLKKYKPDFISLFKNPPKSAQQHERVQKASSEGIPIKGTQRTRILEGQLIKEAFILSDLYNIGEVAAVELLLLGEQQQPTFHGLTRGLVAILLYWDGKSCMADSLLHLIQARKGRTFTLNHSPELVSMVTRFTDDLMEQGLTNKILTLISQIDVNNEFDKLLKDRGLGNEKHRKEVSDLIKECRQSLAHSLYSWSCQTPLNKEDTLLLIGYLEKVTVEGDGSLDKVNLTLLMSLLYCLDVGFLEQATDDREELIKQTSMFMDRQYIAAIHNRLHDTQPWKLPGMQATVRLAWALALRGISQFSEVLEFSEADEPMAELAISGNVFLFLTEAVVGSESFCADEFFIRRIHKLVTDFLTLMPMKVKQLRNRAEEDARLIHMSMQMGNEPPASLRRDLEHLLLLIGELYRIDPFHLELALEYWCPAEPLQSTSLMGSFLGVAHQRPPQRQVVLSKFVRQMSDLLPATLYIPYLKMLRGLASGPQCAHYCFSLLKANGGSSAESLQAAGGSPVSWDHFFHSLMLYHEHLRRDLPSTDSIHLRHPPLRGITQRELDGLIACLQLTLTIVDWSESARLALCEHAQWMPVVVILGLLQCSIPPLLKAELLKTLAAFGKSPEIAASLWQSLEYTQILQTVRTPGLRQGVGIEVELNEIESRCEEYPLTRAFCQLISTLVESSFPTNLGAGLRAPGFEPYLQFLRDTVFLRFRTRAYRRGAEKWEVAEAVLDVFYKLLKDYEPQPEDFVDQYVELQGEERVAFKPPGFSLMHHLLNDSPMLELCLSLMEEGVTQLDTYAPFPGKKHLEKAVAYCFMLLNQTLQKENRFMDLLRESHLSLIVTPLEQLLQGINPRSKKADNVINIARYLYHGNSNPELAFESAKILCSIACNSKIQEKIVGDFTQDQNVTQKLMVGFVSCLDSEEAEELLDSEKEVEDQAKQTNIRYMTKIHILNLLITSLEMKAPNLAMFLLGYELKKPVSTTNLQDSGVLGCPRTCLHSILDILRKGTDGRAGPIAIWDTPHLAELCYQVIYQLCACSDTSGPTMRYLRTSQDFLFTQLQHLPFSVEEYEISAMNQMSWLMKTASIELRITSLNRQRSHTQRLLHLLLDDMPTKPYSAADGEGGMEDESRSLSGFLHFDTTSKVRRKILRILDSIHFSNEIPDPLQLDFFDRSQIEQVIANCEHKNRRGQTVCNVKLLHRVLVAEVNALQGMAAIGQRPLLMEEINTILQYVVERNKLLQCLHAKRHALESWRQLVEIILTACPQDLIPTEHRQLIIRDLLQDLHDKILDDDAAQELMPIVAGAVFTLTTHLSQSVRTELKQPMAAAGLGQSQYVQMLDGSFAAPPGTENLSAGFASIGDSSLHMILRNLLEFILKTGGGFQRVRAHLYGSLLYYLQIAQRPDEPDTLESAHKTMWERLTAPEDVFSKLQRDNLSIFESYGTALMEVVCRDACDGHDIGRMLALALLDRIVSVDRQQWLLYLSNSGYLKVLVDSLAEDDMVLRNLLTPQAPLLKALYIYESKMAFLTRVAKSQQGALELLRSGVIVRLAQCQVYDMRPETDPHGVFGMRETPVFIPAPVERYRQILLPALQICQLILTSSTAQHLQAAGQVLQFLIAHSDTIQAILRSQEGSLGSLQELALLTGIISKAALPGVLNELDIGLNEGSMMELQGHIGRFQRQCLALLNRFGGSDRLRQLSLQDDSSQPDGVSKRDDMELAMQQICSNVMEYCQTLMIQNSPSFQQTVCLFTPNLTESSSRDGTRQDSQVSILPSWRLPGLGIVIHLLKQSANNFFTYYDIHRQSVSKLQNVEQLPPDEIKELCQSEMPVGVDKISTTQKYALARRRLVKLINSRAKLLSLCSYIIETCLFILWRHLEHYLLHCTTSDSQDPVFSNTTFGNRRFQDTINTDPNMDLRNLRQNKVSQQEIDMLLREGANSFGESLQKRLLDIESLYCKVRSRHSFIQALVRRIRGLLRVSRA

Target ORF information:

RefSeq Version	NM_001142124.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nucleoporin 205kDa (nup205), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142124.1

ORF Insert Sequence:

ATGGCGGCGC AGTTGGCGCT AAATTCAGAA GCCAGCCTGT GGGGCCCTTA TAGAGAAATA 
TGGCAGACCG TGGTGGGGGC GCTTGTGAAG AGGCAACCGG AAGCCATTCA TTCGCTCGAT 
ATCGTCTTAA AGAAATATAA ACCGGACTTC ATCTCTCTCT TCAAAAATCC ACCAAAGAGC 
GCTCAGCAAC ATGAAAGAGT GCAGAAAGCC AGCAGCGAGG GGATTCCTAT TAAAGGGACG 
CAGCGGACCC GGATCCTGGA AGGACAGCTC ATTAAGGAAG CCTTCATCCT TAGCGACCTG 
TATAATATCG GAGAGGTGGC AGCCGTAGAA CTTCTACTTT TAGGTGAGCA GCAGCAGCCT 
ACCTTCCATG GCCTGACGAG GGGCTTGGTA GCCATACTGT TGTACTGGGA TGGAAAGAGC 
TGCATGGCTG ATTCCTTATT ACACCTCATC CAGGCAAGAA AAGGCAGGAC CTTCACCTTG 
AATCACAGCC CCGAGCTTGT GTCCATGGTA ACTCGTTTCA CCGATGATCT AATGGAGCAA 
GGCTTGACTA ATAAAATCCT CACCTTGATC TCTCAAATTG ACGTGAACAA TGAGTTTGAC 
AAGTTGCTGA AGGATCGGGG CCTGGGCAAC GAGAAGCATC GGAAAGAGGT TTCTGATCTG 
ATAAAGGAAT GTCGGCAGTC GCTCGCACAC AGCCTGTATT CATGGAGTTG CCAGACACCA 
CTAAACAAAG AAGATACACT ACTTTTGATT GGATACTTGG AAAAAGTAAC AGTAGAAGGA 
GATGGCTCAT TGGACAAAGT GAACTTGACC CTACTGATGT CACTTCTCTA TTGCTTGGAT 
GTTGGCTTTT TGGAGCAAGC GACAGATGAT CGAGAAGAAC TGATAAAGCA GACCAGCATG 
TTCATGGACA GACAGTACAT TGCCGCCATC CATAACCGCC TGCACGACAC TCAGCCGTGG 
AAGCTGCCGG GAATGCAGGC TACGGTCAGA CTCGCTTGGG CCCTGGCTCT TCGAGGGATC 
TCTCAGTTCT CAGAAGTCTT AGAATTTTCA GAAGCAGACG AGCCAATGGC AGAGCTAGCG 
ATCAGCGGCA ATGTCTTCCT CTTCCTCACG GAGGCAGTGG TGGGATCCGA AAGCTTCTGT 
GCAGATGAGT TCTTTATACG AAGGATTCAC AAACTCGTCA CCGATTTCCT TACTCTCATG 
CCCATGAAAG TAAAGCAGCT GAGAAATCGT GCAGAAGAGG ATGCCCGACT GATCCATATG 
AGCATGCAGA TGGGAAATGA GCCCCCTGCT TCTCTGCGTA GGGATTTGGA ACATCTGCTC 
TTACTGATCG GTGAGCTCTA CCGGATAGAC CCATTCCACT TGGAACTGGC GTTGGAATAC 
TGGTGCCCCG CAGAGCCTCT CCAGTCGACA TCCTTGATGG GATCCTTTCT TGGTGTGGCC 
CACCAGAGAC CACCTCAACG TCAGGTCGTC TTGTCCAAGT TCGTGAGACA GATGAGTGAC 
CTGCTGCCAG CCACGCTCTA CATACCTTAT CTTAAGATGC TCAGGGGCCT TGCAAGTGGC 
CCTCAGTGCG CCCATTACTG TTTCAGTCTG CTAAAGGCCA ATGGGGGCAG CAGCGCAGAG 
AGCTTGCAAG CTGCAGGGGG TAGCCCAGTG TCCTGGGACC ACTTCTTCCA CTCTTTGATG 
CTGTACCACG AGCACTTGCG GAGGGATCTA CCAAGCACAG ACAGTATACA CCTGCGTCAC 
CCTCCTCTCC GTGGTATCAC CCAGCGAGAG CTCGATGGCC TGATTGCCTG CTTGCAGCTC 
ACCCTTACCA TCGTCGATTG GAGTGAAAGT GCCCGCCTTG CTCTGTGTGA GCATGCCCAG 
TGGATGCCAG TGGTCGTTAT TCTTGGCCTC CTGCAGTGCA GCATCCCACC ACTCCTTAAG 
GCTGAGCTCC TGAAGACACT GGCTGCATTT GGCAAATCCC CAGAGATCGC AGCATCCCTC 
TGGCAATCCC TCGAATATAC ACAGATTTTG CAGACTGTAA GAACCCCAGG GCTACGGCAA 
GGAGTCGGGA TTGAGGTGGA ACTGAATGAG ATCGAGTCTC GGTGTGAGGA GTACCCCCTG 
ACCAGAGCAT TCTGCCAGCT CATTAGCACT TTAGTGGAGA GCTCCTTCCC CACCAATCTG 
GGAGCGGGTT TGAGAGCACC CGGCTTTGAG CCATACCTCC AGTTTCTACG GGACACTGTG 
TTCCTTCGGT TCCGCACTAG AGCCTACAGG AGAGGCGCAG AAAAGTGGGA GGTAGCAGAG 
GCTGTACTGG ATGTCTTCTA CAAGCTTTTG AAGGACTATG AGCCCCAGCC TGAAGACTTT 
GTGGACCAGT ATGTGGAACT CCAAGGAGAA GAAAGAGTTG CTTTCAAGCC CCCGGGGTTC 
AGCCTCATGC ATCACCTTCT CAATGATTCT CCGATGCTGG AGCTCTGCCT CAGCCTCATG 
GAAGAGGGGG TCACCCAGCT GGACACTTAC GCCCCTTTTC CAGGCAAGAA ACACCTAGAG 
AAAGCAGTGG CTTATTGTTT CATGCTCCTC AATCAGACTC TGCAGAAGGA GAATCGCTTC 
ATGGATTTGT TGAGGGAAAG TCACCTGTCG TTGATTGTGA CCCCTCTGGA GCAGCTCCTG 
CAGGGAATTA ACCCACGATC CAAGAAAGCT GATAATGTCA TCAATATTGC TCGATATCTC 
TACCATGGGA ACAGCAATCC CGAGCTTGCC TTTGAAAGTG CAAAGATCTT ATGCAGCATC 
GCCTGTAACT CCAAGATCCA AGAAAAGATT GTAGGAGACT TTACCCAAGA TCAGAACGTG 
ACCCAGAAGC TGATGGTTGG GTTTGTTTCA TGCCTGGACA GCGAGGAAGC AGAGGAGTTA 
CTGGATTCAG AGAAAGAGGT GGAGGACCAA GCCAAGCAGA CGAACATCCG GTACATGACA 
AAGATTCACA TCCTCAATCT TCTGATCACC TCCCTGGAAA TGAAAGCCCC CAACCTTGCC 
ATGTTCCTTC TCGGCTACGA ACTGAAGAAG CCAGTCAGCA CGACCAACCT GCAGGACTCT 
GGTGTCCTGG GCTGCCCGCG GACCTGCCTT CACTCCATTT TAGACATCTT GAGAAAGGGG 
ACTGATGGGC GGGCTGGACC CATAGCCATA TGGGATACTC CTCATCTTGC TGAACTGTGT 
TACCAGGTGA TCTACCAGCT GTGCGCTTGT TCAGATACCT CTGGCCCCAC CATGAGATAC 
CTACGGACCA GCCAGGATTT CCTCTTCACT CAGCTTCAGC ACCTGCCCTT CTCTGTGGAA 
GAGTATGAGA TCTCCGCCAT GAATCAGATG TCCTGGCTGA TGAAGACGGC GTCCATAGAA 
CTGCGCATCA CCTCCCTAAA CCGCCAACGT TCCCACACGC AGAGGCTCCT TCACCTGCTG 
CTTGACGACA TGCCCACCAA ACCGTATTCA GCAGCTGATG GCGAAGGAGG AATGGAGGAC 
GAAAGCCGCA GCCTCAGTGG ATTTCTGCAC TTTGACACTA CCTCAAAAGT CCGCCGGAAA 
ATCCTCCGCA TCCTGGACTC CATACATTTC AGCAATGAGA TCCCGGATCC CCTGCAGTTG 
GATTTCTTCG ATCGCAGCCA GATAGAGCAG GTTATTGCTA ACTGCGAGCA CAAGAACCGC 
AGAGGGCAGA CCGTCTGCAA CGTCAAGCTT CTCCATCGGG TTCTGGTTGC AGAAGTGAAT 
GCCCTTCAGG GAATGGCTGC TATTGGTCAG AGGCCGCTGC TTATGGAGGA AATCAACACC 
ATCCTTCAGT ACGTGGTGGA AAGGAACAAG CTGCTGCAGT GCCTCCACGC CAAACGCCAT 
GCGCTGGAAT CATGGCGGCA ACTTGTGGAG ATCATACTGA CCGCCTGTCC CCAGGACCTC 
ATTCCGACTG AGCACAGACA GCTCATCATA CGAGACCTGC TGCAAGATCT GCATGATAAG 
ATCCTCGACG ACGACGCTGC CCAAGAACTA ATGCCCATTG TCGCCGGGGC AGTCTTCACC 
CTCACCACTC ATCTCAGCCA ATCTGTACGT ACCGAGCTGA AACAGCCGAT GGCTGCCGCT 
GGGCTGGGCC AGTCGCAATA TGTGCAAATG CTGGACGGCT CATTCGCTGC CCCTCCTGGG 
ACAGAAAACC TCTCCGCTGG TTTTGCATCC ATTGGAGACT CCTCCCTTCA CATGATTCTC 
AGGAATCTGC TCGAGTTCAT TCTAAAAACA GGAGGTGGGT TCCAACGGGT CAGGGCCCAC 
CTGTACGGAT CGCTGCTGTA TTACCTGCAG ATAGCTCAGA GACCAGATGA ACCAGATACT 
CTGGAATCCG CACACAAGAC TATGTGGGAG CGACTGACGG CCCCGGAGGA TGTATTCAGC 
AAGCTGCAGC GAGACAACCT GTCCATATTC GAGAGCTACG GCACCGCCCT TATGGAAGTG 
GTTTGCCGGG ACGCCTGTGA CGGACATGAC ATTGGGAGGA TGCTGGCCCT GGCACTGCTT 
GACCGGATTG TCTCTGTGGA TAGGCAACAG TGGCTGCTTT ATCTCTCCAA CAGTGGCTAC 
CTGAAGGTCC TTGTGGATAG CCTGGCAGAG GACGATATGG TCCTAAGGAA CCTGCTAACT 
CCTCAGGCGC CTCTTCTAAA GGCTCTCTAT ATCTATGAGT CGAAAATGGC ATTTCTGACC 
CGGGTGGCTA AAAGCCAGCA GGGAGCCCTG GAGCTGCTGA GGAGTGGGGT TATCGTCAGG 
CTGGCACAGT GCCAAGTATA TGATATGCGC CCGGAGACTG ACCCCCATGG AGTGTTTGGC 
ATGAGAGAGA CCCCGGTGTT TATCCCCGCC CCGGTGGAAC GATACCGGCA AATCCTGTTA 
CCCGCTCTCC AGATCTGTCA GCTCATCCTG ACCTCCAGCA CGGCACAGCA CTTACAGGCC 
GCCGGACAGG TCTTGCAGTT CCTGATCGCT CATTCTGATA CCATCCAGGC AATTCTCCGG 
AGCCAGGAGG GGAGTCTGGG TTCCTTGCAG GAGTTGGCTT TGCTTACAGG CATCATCAGC 
AAGGCCGCAC TACCCGGTGT TCTCAACGAG CTCGATATCG GTTTAAACGA AGGGTCCATG 
ATGGAGTTAC AAGGACATAT TGGCCGCTTC CAGCGCCAGT GTCTTGCGTT ATTAAACCGC 
TTTGGGGGGT CCGACCGACT TCGGCAGCTC AGTCTGCAGG ATGATTCCTC CCAGCCGGAC 
GGTGTGAGCA AGAGGGACGA CATGGAGTTG GCCATGCAAC AGATCTGCTC CAACGTGATG 
GAGTACTGCC AGACGCTTAT GATACAGAAC TCGCCAAGCT TCCAGCAGAC TGTGTGTCTC 
TTCACCCCCA ACCTCACGGA GAGCTCCAGC CGAGATGGAA CACGGCAGGA TTCCCAGGTG 
TCCATTCTGC CGTCATGGCG GTTGCCAGGC TTGGGTATAG TGATCCACCT TCTGAAGCAG 
AGCGCCAATA ACTTCTTCAC TTACTATGAT ATCCACCGGC AGAGCGTCAG CAAGCTGCAG 
AACGTGGAGC AACTCCCGCC CGATGAGATT AAAGAGCTGT GCCAGTCTGA GATGCCGGTT 
GGCGTAGATA AAATCTCGAC GACGCAGAAG TACGCGCTGG CACGGCGGCG ACTCGTGAAG 
CTTATCAACA GCAGAGCCAA ACTGCTTTCT CTTTGTAGTT ATATCATAGA AACGTGTCTG 
TTCATTCTGT GGCGCCACTT GGAACATTAC CTTCTGCACT GCACCACCAG CGACTCCCAG 
GACCCCGTCT TCTCGAATAC GACCTTCGGC AACAGAAGGT TTCAAGATAC AATTAACACT 
GATCCTAACA TGGACCTGAG GAACCTGAGA CAGAATAAAG TCAGCCAACA GGAAATTGAT 
ATGCTGCTAA GAGAAGGCGC CAATAGTTTC GGGGAGTCCC TGCAGAAGAG ACTGTTAGAC 
ATCGAATCAT TGTACTGCAA GGTGCGATCC CGTCACAGCT TCATCCAGGC GCTGGTCAGG 
CGGATCCGGG GCCTCCTGCG AGTATCAAGG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup205 ( XM_012958683.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814137.1 Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X1, mRNA.

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CloneID	OXa32633
Clone ID Related Accession (Same CDS sequence)	XM_012958683.2
Accession Version	XM_012958683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear pore complex protein Nup205 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958683.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC AGTTGGCGCT AAATTCAGAA GCCAGCCTGT GGGGCCCTTA TAGAGAAATA 
TGGCAGACCG TGGTGGGCGC GCTTGTGAAG AGGCAACCGG AAGCCATTCA TTCGCTCGAT 
ATCGTCTTAA AGAAATATAA ACCGGACTTC ATCTCTCTCT TCAAAAATCC ACCAAAGAGC 
GCTCAGCAAC ATGAAAGAGT GCAGAAAGCC AGCAGCGAGG GGATTCCTAT TAAAGGGACG 
CAGCGGACCC GGATCCTGGA AGGACAGCTC ATTAAGGAAG CCTTCATCCT TAGCGACCTG 
TATAATATCG GAGAGGTGGC AGCCGTAGAA CTTCTACTTT TAGGTGAGCA GCAGCAGCCT 
ACCTTCCATG GCCTGACGAG GGGCTTGGTA GCCATACTGT TGTACTGGGA TGGAAAGAGC 
TGCATGGCTG ATTCCTTATT ACACCTCATC CAGGCAAGAA AAGGCAGGAC CTTCACCTTG 
AATCACAGCC CCGAGCTTGT GTCCATGGTA ACTCGTTTCA CCGATGATCT AATGGAGCAA 
GGCTTGACTA ATAAAATCCT CACCTTGATC TCTCAAATTG ACGTGAACAA TGAGTTTGAC 
AAGTTGCTGA AGGATCGGGG CCTGGGCAAC GAGAAGCATC GGAAAGAGGT TTCTGATCTG 
ATAAAGGAAT GTCGGCAGTC GCTCGCACAC AGCCTGTATT CATGGAGTTG CCAGACACCA 
CTAAACAAAG AAGATACACT ACTTTTGATT GGATACTTGG AAAAAGTAAC AGTAGAAGGA 
GATGGCTCAT TGGACAAAGT GAACTTGACC CTACTGATGT CACTTCTCTA TTGCTTGGAT 
GTTGGCTTTT TGGAGCAAGC GACAGATGAT CGAGAAGAAC TGATAAAGCA GACCAGCATG 
TTCATGGACA GACAGTACAT TGCCGCCATC CATAACCGCC TGCACGACAC TCAGCCGTGG 
AAGCTGCCGG GAATGCAGGC TACGGTCAGA CTCGCTTGGG CCCTGGCTCT TCGAGGGATC 
TCTCAGTTCT CAGAAGTCTT AGAATTTTCA GAAGCAGACG AGCCAATGGC AGAGCTAGCG 
ATCAGCGGCA ATGTCTTCCT CTTCCTCACG GAGGCAGTGG TGGGATCCGA AAGCTTCTGT 
GCAGATGAGT TCTTTATACG AAGGATTCAC AAACTCGTCA CCGATTTCCT TACTCTCATG 
CCCATGAAAG TAAAGCAGCT GAGAAATCGT GCAGAAGAGG ATGCCCGACT GATCCATATG 
AGCATGCAGA TGGGAAATGA GCCCCCTGCT TCTCTGCGTA GGGATTTGGA ACATCTGCTC 
TTACTGATCG GTGAGCTCTA CCGGATAGAC CCATTCCACT TGGAACTGGC GTTGGAATAC 
TGGTGCCCCG CAGAGCCTCT CCAGTCGACA TCCTTGATGG GATCCTTTCT TGGTGTGGCC 
CACCAGAGAC CACCTCAACG TCAGGTCGTC TTGTCCAAGT TCGTGAGACA GATGAGTGAC 
CTGCTGCCAG CCACGCTCTA CATACCTTAT CTTAAGATGC TCAGGGGCCT TGCAAGTGGC 
CCTCAGTGCG CCCATTACTG TTTCAGTCTG CTAAAGGCCA ATGGTGGCAG CAGCGCAGAG 
AGCTTGCAAG CTGCAGGGGG TAGCCCAGTG TCCTGGGACC ACTTCTTCCA CTCTTTGATG 
CTGTACCACG AGCACTTGCG GAGGGATCTA CCAAGCACAG ACAGTATACA CCTGCGTCAC 
CCTCCTCTCC GTGGTATCAC CCAGCGAGAG CTCGATGGCC TGATTGCCTG CTTGCAGCTC 
ACCCTTACCA TCGTCGATTG GAGTGAAAGT GCCCGCCTTG CTCTGTGTGA GCATGCCCAG 
TGGATGCCAG TGGTCGTTAT TCTTGGCCTC CTGCAGTGCA GCATCCCACC ACTCCTTAAG 
GCTGAGCTCC TGAAGACACT GGCTGCATTT GGCAAATCCC CAGAGATCGC AGCATCCCTC 
TGGCAATCCC TCGAATATAC ACAGATTTTG CAGACTGTAA GAACCCCAGG GCTACGGCAA 
GGAGTCGGGA TTGAGGTGGA ACTGAATGAG ATCGAGTCTC GGTGTGAGGA GTACCCCCTG 
ACCAGAGCAT TCTGCCAGCT CATTAGCACT TTAGTGGAGA GCTCCTTCCC CACCAATCTG 
GGAGCGGGTT TGAGAGCACC CGGCTTTGAG CCATACCTCC AGTTTCTACG GGACACTGTG 
TTCCTTCGGT TCCGCACTAG AGCCTACAGG AGAGGCGCAG AAAAGTGGGA GGTAGCAGAG 
GCTGTACTGG ATGTCTTCTA CAAGCTTTTG AAGGACTATG AGCCCCAGCC TGAAGACTTT 
GTGGACCAGT ATGTGGAACT CCAAGGAGAA GAAAGAGTTG CTTTCAAGCC CCCGGGGTTC 
AGCCTCATGC ATCACCTTCT CAATGATTCT CCGATGCTGG AGCTCTGCCT CAGCCTCATG 
GAAGAGGGGG TCACCCAGCT GGACACTTAC GCCCCTTTTC CAGGCAAGAA ACACCTAGAG 
AAAGCAGTGG CTTATTGTTT CATGCTCCTC AATCAGACTC TGCAGAAGGA GAATCGCTTC 
ATGGATTTGT TGAGGGAAAG TCACCTGTCG TTGATTGTGA CCCCTCTGGA GCAGCTCCTG 
CAGGGAATTA ACCCACGATC TAAGAAAGCC GATAATGTCA TCAATATTGC TCGATATCTC 
TACCATGGGA ACAGCAATCC CGAGCTTGCC TTTGAAAGTG CAAAGATCTT ATGCAGCATC 
GCCTGTAACT CCAAGATCCA AGAAAAGATT GTAGGAGACT TTACCCAAGA TCAGAACGTG 
ACCCAGAAGC TGATGGTTGG GTTTGTTTCA TGCCTGGACA GCGAGGAAGC AGAGGAGTTA 
CTGGATTCAG AGAAAGAGGT GGAGGATCAA GCCAAGCAGA CGAACATCCG GTACATGACA 
AAGATTCACA TCCTCAATCT TCTGATCACC TCCCTGGAAA TGAAAGCCCC CAACCTTGCC 
ATGTTCCTTC TCGGCTACGA ACTGAAGAAG CCAGTCAGCA CGACCAACCT GCAGGACTCT 
GGTGTCCTGG GCTGCCCGCG GACCTGCCTT CACTCCATTT TAGACATCTT GAGAAAGGGG 
ACTGATGGGC GGGCTGGACC CATAGCCATA TGGGATACTC CTCATCTTGC TGAACTGTGT 
TACCAGGTGA TCTACCAGCT GTGCGCTTGT TCCGATACCT CTGGCCCCAC CATGAGATAC 
CTACGGACCA GCCAGGATTT CCTCTTCACT CAGCTTCAGC ACCTGCCCTT CTCTGTGGAA 
GAGTATGAGA TCTCCGCCAT GAATCAGATG TCCTGGCTGA TGAAGACTGC GTCCATAGAA 
CTGCGCATCA CCTCCCTAAA CCGCCAACGT TCCCACACGC AGAGGCTCCT TCACCTGCTG 
CTTGACGACA TGCCCACCAA ACCGTATTCA GCTGATGGCG AAGGAGGAAT GGAGGACGAA 
AGCCGCAGCC TCAGTGGATT TCTGCACTTT GACACTACCT CAAAAGTCCG CCGGAAAATC 
CTCCGCATCC TGGACTCCAT ACATTTCAGC AATGAGATCC CGGATCCCCT GCAGTTGGAT 
TTCTTCGATC GCAGCCAGAT AGAGCAGGTT ATTGCTAACT GCGAGCACAA GAACCGCAGA 
GGGCAGACCG TCTGCAACGT CAAGCTTCTC CATCGGGTTC TGGTTGCAGA AGTGAATGCC 
CTTCAGGGAA TGGCTGCTAT TGGTCAGAGG CCGCTGCTTA TGGAGGAAAT CAACACCATC 
CTTCAGTACG TGGTGGAAAG GAACAAGCTG CTGCAGTGCC TCCACGCCAA ACGCCATGCG 
CTGGAATCAT GGCGGCAACT TGTGGAGATC ATACTGACCG CCTGTCCCCA GGACCTCATT 
CCGACTGAGC ACAGACAGCT CATCATACGA GACCTGCTGC AAGATCTGCA TGATAAGATC 
CTCGACGACG ACGCTGCCCA AGAACTAATG CCCATTGTCG CCGGGGCAGT CTTCACCCTC 
ACCACTCATC TCAGCCAATC TGTACGTACC GAGCTGAAAC AGCCGATGGC TGCCGCTGGG 
CTGGGCCAGT CGCAATATGT GCAAATGCTG GACGGCTCAT TCGCTGCCCC TCCTGGGACA 
GAAAACCTCT CCGCTGGTTT TGCATCCATT GGAGACTCCT CCCTTCACAT GATTCTCAGG 
AATCTGCTCG AGTTCATTCT AAAAACAGGA GGTGGGTTCC AACGGGTCAG GGCCCACCTG 
TACGGATCGC TGCTGTATTA CCTGCAGATA GCTCAGAGAC CAGATGAACC AGATACTCTG 
GAATCCGCAC ACAAGACTAT GTGGGAGCGA CTGACGGCCC CGGAGGATGT ATTCAGCAAG 
CTGCAGCGAG ACAACCTGTC CATATTCGAG AGCTACGGCA CCGCCCTTAT GGAAGTGGTT 
TGCCGGGACG CCTGTGACGG ACATGACATT GGGAGGATGC TGGCCCTGGC ACTGCTTGAC 
CGGATTGTCT CTGTGGATAG GCAACAGTGG CTGCTTTATC TCTCCAACAG TGGCTACCTG 
AAGGTCCTTG TGGATAGCCT GGCAGAGGAC GATATGGTCC TAAGGAACCT GCTAACTCCT 
CAGGCGCCTC TTCTAAAGGC TCTCTATATC TATGAGTCGA AAATGGCATT TCTGACCCGG 
GTGGCTAAAA GCCAGCAGGG AGCCCTGGAG CTGCTGAGGA GTGGGGTTAT CGTCAGGCTG 
GCACAGTGCC AAGTATATGA TATGCGCCCG GAGACTGACC CCCATGGAGT GTTTGGCATG 
AGAGAGACCC CGGTGTTTAT CCCCGCCCCG GTGGAACGAT ACCGGCAAAT CCTGTTACCC 
GCTCTCCAGA TCTGTCAGCT CATCCTGACC TCCAGCACGG CACAGCACTT ACAGGCCGCC 
GGACAGGTCT TGCAGTTCCT GATCGCTCAT TCTGATACCA TCCAGGCAAT TCTCCGGAGC 
CAGGAGGGGA GTCTGGGTTC CTTGCAGGAG TTGGCTTTGC TTACAGGCAT CATCAGCAAG 
GCCGCACTAC CCGGTGTTCT CAACGAGCTC GATATCGGTT TAAACGAAGG GTCCATGATG 
GAGTTACAAG GACATATTGG CCGCTTCCAG CGCCAGTGTC TTGCGTTATT AAACCGCTTT 
GGGGGGTCCG ACCGACTTCG GCAGCTCAGT CTGCAGGATG ATTCCTCCCA GCCGGACGGT 
GTGAGCAAGA GGGACGACAT GGAGTTGGCC ATGCAACAGA TCTGCTCCAA CGTGATGGAG 
TACTGCCAGA CGCTTATGAT ACAGAACTCG CCAAGCTTCC AGCAGACTGT GTGTCTCTTC 
ACCCCCAACC TCACGGAGAG CTCCAGCCGA GATGGAACAC GGCAGGATTC CCAGGTGTCC 
ATTCTGCCGT CATGGCGGTT GCCAGGCTTG GGTATAGTGA TCCACCTTCT GAAGCAGAGC 
GCCAATAACT TCTTCACTTA CTATGATATC CACCGGCAGA GCGTCAGCAA GCTGCAGAAC 
GTGGAGCAAC TCCCGCCCGA TGAGATTAAA GAGCTGTGCC AGTCTGAGAT GCCGGTTGGC 
GTAGATAAAA TCTCGACGAC GCAGAAGTAC GCGCTGGCAC GGCGGCGACT CGTGAAGCTT 
ATCAACAGCA GAGCCAAACT GCTTTCTCTT TGTAGTTATA TCATAGAAAC GTGTCTGTTC 
ATTCTGTGGC GCCACTTGGA ACATTACCTT CTGCACTGCA CCACCAGCGA CTCCCAGGAC 
CCCGTCTTCT CGAATACGAC CTTCGGCAAC AGAAGGTTTC AAGATACAAT TAACACTGAT 
CCTAACATGG ACCTGAGGAA CCTGAGACAG AATAAAGTCA GCCAACAGGA AATTGATATG 
CTGCTAAGAG AAGGCGCCAA TAGTTTCGGG GAGTCCCTGC AGAAGAGACT GTTAGACATC 
GAATCATTGT ACTGCAAGGT GCGATCCCGT CACAGCTTCA TCCAGGCGCT GGTCAGGCGG 
ATCCGGGGCC TCCTGCGAGT ATCAAGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814137.1
CDS	232..6264
Translation	MAAQLALNSEASLWGPYREIWQTVVGALVKRQPEAIHSLDIVLKKYKPDFISLFKNPPKSAQQHERVQKASSEGIPIKGTQRTRILEGQLIKEAFILSDLYNIGEVAAVELLLLGEQQQPTFHGLTRGLVAILLYWDGKSCMADSLLHLIQARKGRTFTLNHSPELVSMVTRFTDDLMEQGLTNKILTLISQIDVNNEFDKLLKDRGLGNEKHRKEVSDLIKECRQSLAHSLYSWSCQTPLNKEDTLLLIGYLEKVTVEGDGSLDKVNLTLLMSLLYCLDVGFLEQATDDREELIKQTSMFMDRQYIAAIHNRLHDTQPWKLPGMQATVRLAWALALRGISQFSEVLEFSEADEPMAELAISGNVFLFLTEAVVGSESFCADEFFIRRIHKLVTDFLTLMPMKVKQLRNRAEEDARLIHMSMQMGNEPPASLRRDLEHLLLLIGELYRIDPFHLELALEYWCPAEPLQSTSLMGSFLGVAHQRPPQRQVVLSKFVRQMSDLLPATLYIPYLKMLRGLASGPQCAHYCFSLLKANGGSSAESLQAAGGSPVSWDHFFHSLMLYHEHLRRDLPSTDSIHLRHPPLRGITQRELDGLIACLQLTLTIVDWSESARLALCEHAQWMPVVVILGLLQCSIPPLLKAELLKTLAAFGKSPEIAASLWQSLEYTQILQTVRTPGLRQGVGIEVELNEIESRCEEYPLTRAFCQLISTLVESSFPTNLGAGLRAPGFEPYLQFLRDTVFLRFRTRAYRRGAEKWEVAEAVLDVFYKLLKDYEPQPEDFVDQYVELQGEERVAFKPPGFSLMHHLLNDSPMLELCLSLMEEGVTQLDTYAPFPGKKHLEKAVAYCFMLLNQTLQKENRFMDLLRESHLSLIVTPLEQLLQGINPRSKKADNVINIARYLYHGNSNPELAFESAKILCSIACNSKIQEKIVGDFTQDQNVTQKLMVGFVSCLDSEEAEELLDSEKEVEDQAKQTNIRYMTKIHILNLLITSLEMKAPNLAMFLLGYELKKPVSTTNLQDSGVLGCPRTCLHSILDILRKGTDGRAGPIAIWDTPHLAELCYQVIYQLCACSDTSGPTMRYLRTSQDFLFTQLQHLPFSVEEYEISAMNQMSWLMKTASIELRITSLNRQRSHTQRLLHLLLDDMPTKPYSADGEGGMEDESRSLSGFLHFDTTSKVRRKILRILDSIHFSNEIPDPLQLDFFDRSQIEQVIANCEHKNRRGQTVCNVKLLHRVLVAEVNALQGMAAIGQRPLLMEEINTILQYVVERNKLLQCLHAKRHALESWRQLVEIILTACPQDLIPTEHRQLIIRDLLQDLHDKILDDDAAQELMPIVAGAVFTLTTHLSQSVRTELKQPMAAAGLGQSQYVQMLDGSFAAPPGTENLSAGFASIGDSSLHMILRNLLEFILKTGGGFQRVRAHLYGSLLYYLQIAQRPDEPDTLESAHKTMWERLTAPEDVFSKLQRDNLSIFESYGTALMEVVCRDACDGHDIGRMLALALLDRIVSVDRQQWLLYLSNSGYLKVLVDSLAEDDMVLRNLLTPQAPLLKALYIYESKMAFLTRVAKSQQGALELLRSGVIVRLAQCQVYDMRPETDPHGVFGMRETPVFIPAPVERYRQILLPALQICQLILTSSTAQHLQAAGQVLQFLIAHSDTIQAILRSQEGSLGSLQELALLTGIISKAALPGVLNELDIGLNEGSMMELQGHIGRFQRQCLALLNRFGGSDRLRQLSLQDDSSQPDGVSKRDDMELAMQQICSNVMEYCQTLMIQNSPSFQQTVCLFTPNLTESSSRDGTRQDSQVSILPSWRLPGLGIVIHLLKQSANNFFTYYDIHRQSVSKLQNVEQLPPDEIKELCQSEMPVGVDKISTTQKYALARRRLVKLINSRAKLLSLCSYIIETCLFILWRHLEHYLLHCTTSDSQDPVFSNTTFGNRRFQDTINTDPNMDLRNLRQNKVSQQEIDMLLREGANSFGESLQKRLLDIESLYCKVRSRHSFIQALVRRIRGLLRVSRA

Target ORF information:

RefSeq Version	XM_012958683.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958683.2

ORF Insert Sequence:

ATGGCGGCGC AGTTGGCGCT AAATTCAGAA GCCAGCCTGT GGGGCCCTTA TAGAGAAATA 
TGGCAGACCG TGGTGGGCGC GCTTGTGAAG AGGCAACCGG AAGCCATTCA TTCGCTCGAT 
ATCGTCTTAA AGAAATATAA ACCGGACTTC ATCTCTCTCT TCAAAAATCC ACCAAAGAGC 
GCTCAGCAAC ATGAAAGAGT GCAGAAAGCC AGCAGCGAGG GGATTCCTAT TAAAGGGACG 
CAGCGGACCC GGATCCTGGA AGGACAGCTC ATTAAGGAAG CCTTCATCCT TAGCGACCTG 
TATAATATCG GAGAGGTGGC AGCCGTAGAA CTTCTACTTT TAGGTGAGCA GCAGCAGCCT 
ACCTTCCATG GCCTGACGAG GGGCTTGGTA GCCATACTGT TGTACTGGGA TGGAAAGAGC 
TGCATGGCTG ATTCCTTATT ACACCTCATC CAGGCAAGAA AAGGCAGGAC CTTCACCTTG 
AATCACAGCC CCGAGCTTGT GTCCATGGTA ACTCGTTTCA CCGATGATCT AATGGAGCAA 
GGCTTGACTA ATAAAATCCT CACCTTGATC TCTCAAATTG ACGTGAACAA TGAGTTTGAC 
AAGTTGCTGA AGGATCGGGG CCTGGGCAAC GAGAAGCATC GGAAAGAGGT TTCTGATCTG 
ATAAAGGAAT GTCGGCAGTC GCTCGCACAC AGCCTGTATT CATGGAGTTG CCAGACACCA 
CTAAACAAAG AAGATACACT ACTTTTGATT GGATACTTGG AAAAAGTAAC AGTAGAAGGA 
GATGGCTCAT TGGACAAAGT GAACTTGACC CTACTGATGT CACTTCTCTA TTGCTTGGAT 
GTTGGCTTTT TGGAGCAAGC GACAGATGAT CGAGAAGAAC TGATAAAGCA GACCAGCATG 
TTCATGGACA GACAGTACAT TGCCGCCATC CATAACCGCC TGCACGACAC TCAGCCGTGG 
AAGCTGCCGG GAATGCAGGC TACGGTCAGA CTCGCTTGGG CCCTGGCTCT TCGAGGGATC 
TCTCAGTTCT CAGAAGTCTT AGAATTTTCA GAAGCAGACG AGCCAATGGC AGAGCTAGCG 
ATCAGCGGCA ATGTCTTCCT CTTCCTCACG GAGGCAGTGG TGGGATCCGA AAGCTTCTGT 
GCAGATGAGT TCTTTATACG AAGGATTCAC AAACTCGTCA CCGATTTCCT TACTCTCATG 
CCCATGAAAG TAAAGCAGCT GAGAAATCGT GCAGAAGAGG ATGCCCGACT GATCCATATG 
AGCATGCAGA TGGGAAATGA GCCCCCTGCT TCTCTGCGTA GGGATTTGGA ACATCTGCTC 
TTACTGATCG GTGAGCTCTA CCGGATAGAC CCATTCCACT TGGAACTGGC GTTGGAATAC 
TGGTGCCCCG CAGAGCCTCT CCAGTCGACA TCCTTGATGG GATCCTTTCT TGGTGTGGCC 
CACCAGAGAC CACCTCAACG TCAGGTCGTC TTGTCCAAGT TCGTGAGACA GATGAGTGAC 
CTGCTGCCAG CCACGCTCTA CATACCTTAT CTTAAGATGC TCAGGGGCCT TGCAAGTGGC 
CCTCAGTGCG CCCATTACTG TTTCAGTCTG CTAAAGGCCA ATGGTGGCAG CAGCGCAGAG 
AGCTTGCAAG CTGCAGGGGG TAGCCCAGTG TCCTGGGACC ACTTCTTCCA CTCTTTGATG 
CTGTACCACG AGCACTTGCG GAGGGATCTA CCAAGCACAG ACAGTATACA CCTGCGTCAC 
CCTCCTCTCC GTGGTATCAC CCAGCGAGAG CTCGATGGCC TGATTGCCTG CTTGCAGCTC 
ACCCTTACCA TCGTCGATTG GAGTGAAAGT GCCCGCCTTG CTCTGTGTGA GCATGCCCAG 
TGGATGCCAG TGGTCGTTAT TCTTGGCCTC CTGCAGTGCA GCATCCCACC ACTCCTTAAG 
GCTGAGCTCC TGAAGACACT GGCTGCATTT GGCAAATCCC CAGAGATCGC AGCATCCCTC 
TGGCAATCCC TCGAATATAC ACAGATTTTG CAGACTGTAA GAACCCCAGG GCTACGGCAA 
GGAGTCGGGA TTGAGGTGGA ACTGAATGAG ATCGAGTCTC GGTGTGAGGA GTACCCCCTG 
ACCAGAGCAT TCTGCCAGCT CATTAGCACT TTAGTGGAGA GCTCCTTCCC CACCAATCTG 
GGAGCGGGTT TGAGAGCACC CGGCTTTGAG CCATACCTCC AGTTTCTACG GGACACTGTG 
TTCCTTCGGT TCCGCACTAG AGCCTACAGG AGAGGCGCAG AAAAGTGGGA GGTAGCAGAG 
GCTGTACTGG ATGTCTTCTA CAAGCTTTTG AAGGACTATG AGCCCCAGCC TGAAGACTTT 
GTGGACCAGT ATGTGGAACT CCAAGGAGAA GAAAGAGTTG CTTTCAAGCC CCCGGGGTTC 
AGCCTCATGC ATCACCTTCT CAATGATTCT CCGATGCTGG AGCTCTGCCT CAGCCTCATG 
GAAGAGGGGG TCACCCAGCT GGACACTTAC GCCCCTTTTC CAGGCAAGAA ACACCTAGAG 
AAAGCAGTGG CTTATTGTTT CATGCTCCTC AATCAGACTC TGCAGAAGGA GAATCGCTTC 
ATGGATTTGT TGAGGGAAAG TCACCTGTCG TTGATTGTGA CCCCTCTGGA GCAGCTCCTG 
CAGGGAATTA ACCCACGATC TAAGAAAGCC GATAATGTCA TCAATATTGC TCGATATCTC 
TACCATGGGA ACAGCAATCC CGAGCTTGCC TTTGAAAGTG CAAAGATCTT ATGCAGCATC 
GCCTGTAACT CCAAGATCCA AGAAAAGATT GTAGGAGACT TTACCCAAGA TCAGAACGTG 
ACCCAGAAGC TGATGGTTGG GTTTGTTTCA TGCCTGGACA GCGAGGAAGC AGAGGAGTTA 
CTGGATTCAG AGAAAGAGGT GGAGGATCAA GCCAAGCAGA CGAACATCCG GTACATGACA 
AAGATTCACA TCCTCAATCT TCTGATCACC TCCCTGGAAA TGAAAGCCCC CAACCTTGCC 
ATGTTCCTTC TCGGCTACGA ACTGAAGAAG CCAGTCAGCA CGACCAACCT GCAGGACTCT 
GGTGTCCTGG GCTGCCCGCG GACCTGCCTT CACTCCATTT TAGACATCTT GAGAAAGGGG 
ACTGATGGGC GGGCTGGACC CATAGCCATA TGGGATACTC CTCATCTTGC TGAACTGTGT 
TACCAGGTGA TCTACCAGCT GTGCGCTTGT TCCGATACCT CTGGCCCCAC CATGAGATAC 
CTACGGACCA GCCAGGATTT CCTCTTCACT CAGCTTCAGC ACCTGCCCTT CTCTGTGGAA 
GAGTATGAGA TCTCCGCCAT GAATCAGATG TCCTGGCTGA TGAAGACTGC GTCCATAGAA 
CTGCGCATCA CCTCCCTAAA CCGCCAACGT TCCCACACGC AGAGGCTCCT TCACCTGCTG 
CTTGACGACA TGCCCACCAA ACCGTATTCA GCTGATGGCG AAGGAGGAAT GGAGGACGAA 
AGCCGCAGCC TCAGTGGATT TCTGCACTTT GACACTACCT CAAAAGTCCG CCGGAAAATC 
CTCCGCATCC TGGACTCCAT ACATTTCAGC AATGAGATCC CGGATCCCCT GCAGTTGGAT 
TTCTTCGATC GCAGCCAGAT AGAGCAGGTT ATTGCTAACT GCGAGCACAA GAACCGCAGA 
GGGCAGACCG TCTGCAACGT CAAGCTTCTC CATCGGGTTC TGGTTGCAGA AGTGAATGCC 
CTTCAGGGAA TGGCTGCTAT TGGTCAGAGG CCGCTGCTTA TGGAGGAAAT CAACACCATC 
CTTCAGTACG TGGTGGAAAG GAACAAGCTG CTGCAGTGCC TCCACGCCAA ACGCCATGCG 
CTGGAATCAT GGCGGCAACT TGTGGAGATC ATACTGACCG CCTGTCCCCA GGACCTCATT 
CCGACTGAGC ACAGACAGCT CATCATACGA GACCTGCTGC AAGATCTGCA TGATAAGATC 
CTCGACGACG ACGCTGCCCA AGAACTAATG CCCATTGTCG CCGGGGCAGT CTTCACCCTC 
ACCACTCATC TCAGCCAATC TGTACGTACC GAGCTGAAAC AGCCGATGGC TGCCGCTGGG 
CTGGGCCAGT CGCAATATGT GCAAATGCTG GACGGCTCAT TCGCTGCCCC TCCTGGGACA 
GAAAACCTCT CCGCTGGTTT TGCATCCATT GGAGACTCCT CCCTTCACAT GATTCTCAGG 
AATCTGCTCG AGTTCATTCT AAAAACAGGA GGTGGGTTCC AACGGGTCAG GGCCCACCTG 
TACGGATCGC TGCTGTATTA CCTGCAGATA GCTCAGAGAC CAGATGAACC AGATACTCTG 
GAATCCGCAC ACAAGACTAT GTGGGAGCGA CTGACGGCCC CGGAGGATGT ATTCAGCAAG 
CTGCAGCGAG ACAACCTGTC CATATTCGAG AGCTACGGCA CCGCCCTTAT GGAAGTGGTT 
TGCCGGGACG CCTGTGACGG ACATGACATT GGGAGGATGC TGGCCCTGGC ACTGCTTGAC 
CGGATTGTCT CTGTGGATAG GCAACAGTGG CTGCTTTATC TCTCCAACAG TGGCTACCTG 
AAGGTCCTTG TGGATAGCCT GGCAGAGGAC GATATGGTCC TAAGGAACCT GCTAACTCCT 
CAGGCGCCTC TTCTAAAGGC TCTCTATATC TATGAGTCGA AAATGGCATT TCTGACCCGG 
GTGGCTAAAA GCCAGCAGGG AGCCCTGGAG CTGCTGAGGA GTGGGGTTAT CGTCAGGCTG 
GCACAGTGCC AAGTATATGA TATGCGCCCG GAGACTGACC CCCATGGAGT GTTTGGCATG 
AGAGAGACCC CGGTGTTTAT CCCCGCCCCG GTGGAACGAT ACCGGCAAAT CCTGTTACCC 
GCTCTCCAGA TCTGTCAGCT CATCCTGACC TCCAGCACGG CACAGCACTT ACAGGCCGCC 
GGACAGGTCT TGCAGTTCCT GATCGCTCAT TCTGATACCA TCCAGGCAAT TCTCCGGAGC 
CAGGAGGGGA GTCTGGGTTC CTTGCAGGAG TTGGCTTTGC TTACAGGCAT CATCAGCAAG 
GCCGCACTAC CCGGTGTTCT CAACGAGCTC GATATCGGTT TAAACGAAGG GTCCATGATG 
GAGTTACAAG GACATATTGG CCGCTTCCAG CGCCAGTGTC TTGCGTTATT AAACCGCTTT 
GGGGGGTCCG ACCGACTTCG GCAGCTCAGT CTGCAGGATG ATTCCTCCCA GCCGGACGGT 
GTGAGCAAGA GGGACGACAT GGAGTTGGCC ATGCAACAGA TCTGCTCCAA CGTGATGGAG 
TACTGCCAGA CGCTTATGAT ACAGAACTCG CCAAGCTTCC AGCAGACTGT GTGTCTCTTC 
ACCCCCAACC TCACGGAGAG CTCCAGCCGA GATGGAACAC GGCAGGATTC CCAGGTGTCC 
ATTCTGCCGT CATGGCGGTT GCCAGGCTTG GGTATAGTGA TCCACCTTCT GAAGCAGAGC 
GCCAATAACT TCTTCACTTA CTATGATATC CACCGGCAGA GCGTCAGCAA GCTGCAGAAC 
GTGGAGCAAC TCCCGCCCGA TGAGATTAAA GAGCTGTGCC AGTCTGAGAT GCCGGTTGGC 
GTAGATAAAA TCTCGACGAC GCAGAAGTAC GCGCTGGCAC GGCGGCGACT CGTGAAGCTT 
ATCAACAGCA GAGCCAAACT GCTTTCTCTT TGTAGTTATA TCATAGAAAC GTGTCTGTTC 
ATTCTGTGGC GCCACTTGGA ACATTACCTT CTGCACTGCA CCACCAGCGA CTCCCAGGAC 
CCCGTCTTCT CGAATACGAC CTTCGGCAAC AGAAGGTTTC AAGATACAAT TAACACTGAT 
CCTAACATGG ACCTGAGGAA CCTGAGACAG AATAAAGTCA GCCAACAGGA AATTGATATG 
CTGCTAAGAG AAGGCGCCAA TAGTTTCGGG GAGTCCCTGC AGAAGAGACT GTTAGACATC 
GAATCATTGT ACTGCAAGGT GCGATCCCGT CACAGCTTCA TCCAGGCGCT GGTCAGGCGG 
ATCCGGGGCC TCCTGCGAGT ATCAAGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup205 ( XM_012958684.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814138.1 Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X2, mRNA.

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CloneID	OXa32634
Clone ID Related Accession (Same CDS sequence)	XM_012958684.2
Accession Version	XM_012958684.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear pore complex protein Nup205 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC AGTTGGCGCT AAATTCAGAA GCCAGCCTGT GGGGCCCTTA TAGAGAAATA 
TGGCAGACCG TGGTGGGCGC GCTTGTGAAG AGGCAACCGG AAGCCATTCA TTCGCTCGAT 
ATCGTCTTAA AGAAATATAA ACCGGACTTC ATCTCTCTCT TCAAAAATCC ACCAAAGAGC 
GCTCAGCAAC ATGAAAGAGT GCAGAAAGCC AGCAGCGAGG GGATTCCTAT TAAAGGGACG 
CAGCGGACCC GGATCCTGGA AGGACAGCTC ATTAAGGAAG CCTTCATCCT TAGCGACCTG 
TATAATATCG GAGAGGTGGC AGCCGTAGAA CTTCTACTTT TAGGTGAGCA GCAGCAGCCT 
ACCTTCCATG GCCTGACGAG GGGCTTGGTA GCCATACTGT TGTACTGGGA TGGAAAGAGC 
TGCATGGCTG ATTCCTTATT ACACCTCATC CAGGCAAGAA AAGGCAGGAC CTTCACCTTG 
AATCACAGCC CCGAGCTTGT GTCCATGGTA ACTCGTTTCA CCGATGATCT AATGGAGCAA 
GGCTTGACTA ATAAAATCCT CACCTTGATC TCTCAAATTG ACGTGAACAA TGAGTTTGAC 
AAGTTGCTGA AGGATCGGGG CCTGGGCAAC GAGAAGCATC GGAAAGAGGT TTCTGATCTG 
ATAAAGGAAT GTCGGCAGTC GCTCGCACAC AGCCTGTATT CATGGAGTTG CCAGACACCA 
CTAAACAAAG AAGATACACT ACTTTTGATT GGATACTTGG AAAAAGTAAC AGTAGAAGGA 
GATGGCTCAT TGGACAAAGT GAACTTGACC CTACTGATGT CACTTCTCTA TTGCTTGGAT 
GTTGGCTTTT TGGAGCAAGC GACAGATGAT CGAGAAGAAC TGATAAAGCA GACCAGCATG 
TTCATGGACA GACAGTACAT TGCCGCCATC CATAACCGCC TGCACGACAC TCAGCCGTGG 
AAGCTGCCGG GAATGCAGGC TACGGTCAGA CTCGCTTGGG CCCTGGCTCT TCGAGGGATC 
TCTCAGTTCT CAGAAGTCTT AGAATTTTCA GAAGCAGACG AGCCAATGGC AGAGCTAGCG 
ATCAGCGGCA ATGTCTTCCT CTTCCTCACG GAGGCAGTGG TGGGATCCGA AAGCTTCTGT 
GCAGATGAGT TCTTTATACG AAGGATTCAC AAACTCGTCA CCGATTTCCT TACTCTCATG 
CCCATGAAAG TAAAGCAGCT GAGAAATCGT GCAGAAGAGG ATGCCCGACT GATCCATATG 
AGCATGCAGA TGGGAAATGA GCCCCCTGCT TCTCTGCGTA GGGATTTGGA ACATCTGCTC 
TTACTGATCG GTGAGCTCTA CCGGATAGAC CCATTCCACT TGGAACTGGC GTTGGAATAC 
TGGTGCCCCG CAGAGCCTCT CCAGTCGACA TCCTTGATGG GATCCTTTCT TGGTGTGGCC 
CACCAGAGAC CACCTCAACG TCAGGTCGTC TTGTCCAAGT TCGTGAGACA GATGAGTGAC 
CTGCTGCCAG CCACGCTCTA CATACCTTAT CTTAAGATGC TCAGGGGCCT TGCAAGTGGC 
CCTCAGTGCG CCCATTACTG TTTCAGTCTG CTAAAGGCCA ATGGTGGCAG CAGCGAGAGC 
TTGCAAGCTG CAGGGGGTAG CCCAGTGTCC TGGGACCACT TCTTCCACTC TTTGATGCTG 
TACCACGAGC ACTTGCGGAG GGATCTACCA AGCACAGACA GTATACACCT GCGTCACCCT 
CCTCTCCGTG GTATCACCCA GCGAGAGCTC GATGGCCTGA TTGCCTGCTT GCAGCTCACC 
CTTACCATCG TCGATTGGAG TGAAAGTGCC CGCCTTGCTC TGTGTGAGCA TGCCCAGTGG 
ATGCCAGTGG TCGTTATTCT TGGCCTCCTG CAGTGCAGCA TCCCACCACT CCTTAAGGCT 
GAGCTCCTGA AGACACTGGC TGCATTTGGC AAATCCCCAG AGATCGCAGC ATCCCTCTGG 
CAATCCCTCG AATATACACA GATTTTGCAG ACTGTAAGAA CCCCAGGGCT ACGGCAAGGA 
GTCGGGATTG AGGTGGAACT GAATGAGATC GAGTCTCGGT GTGAGGAGTA CCCCCTGACC 
AGAGCATTCT GCCAGCTCAT TAGCACTTTA GTGGAGAGCT CCTTCCCCAC CAATCTGGGA 
GCGGGTTTGA GAGCACCCGG CTTTGAGCCA TACCTCCAGT TTCTACGGGA CACTGTGTTC 
CTTCGGTTCC GCACTAGAGC CTACAGGAGA GGCGCAGAAA AGTGGGAGGT AGCAGAGGCT 
GTACTGGATG TCTTCTACAA GCTTTTGAAG GACTATGAGC CCCAGCCTGA AGACTTTGTG 
GACCAGTATG TGGAACTCCA AGGAGAAGAA AGAGTTGCTT TCAAGCCCCC GGGGTTCAGC 
CTCATGCATC ACCTTCTCAA TGATTCTCCG ATGCTGGAGC TCTGCCTCAG CCTCATGGAA 
GAGGGGGTCA CCCAGCTGGA CACTTACGCC CCTTTTCCAG GCAAGAAACA CCTAGAGAAA 
GCAGTGGCTT ATTGTTTCAT GCTCCTCAAT CAGACTCTGC AGAAGGAGAA TCGCTTCATG 
GATTTGTTGA GGGAAAGTCA CCTGTCGTTG ATTGTGACCC CTCTGGAGCA GCTCCTGCAG 
GGAATTAACC CACGATCTAA GAAAGCCGAT AATGTCATCA ATATTGCTCG ATATCTCTAC 
CATGGGAACA GCAATCCCGA GCTTGCCTTT GAAAGTGCAA AGATCTTATG CAGCATCGCC 
TGTAACTCCA AGATCCAAGA AAAGATTGTA GGAGACTTTA CCCAAGATCA GAACGTGACC 
CAGAAGCTGA TGGTTGGGTT TGTTTCATGC CTGGACAGCG AGGAAGCAGA GGAGTTACTG 
GATTCAGAGA AAGAGGTGGA GGATCAAGCC AAGCAGACGA ACATCCGGTA CATGACAAAG 
ATTCACATCC TCAATCTTCT GATCACCTCC CTGGAAATGA AAGCCCCCAA CCTTGCCATG 
TTCCTTCTCG GCTACGAACT GAAGAAGCCA GTCAGCACGA CCAACCTGCA GGACTCTGGT 
GTCCTGGGCT GCCCGCGGAC CTGCCTTCAC TCCATTTTAG ACATCTTGAG AAAGGGGACT 
GATGGGCGGG CTGGACCCAT AGCCATATGG GATACTCCTC ATCTTGCTGA ACTGTGTTAC 
CAGGTGATCT ACCAGCTGTG CGCTTGTTCC GATACCTCTG GCCCCACCAT GAGATACCTA 
CGGACCAGCC AGGATTTCCT CTTCACTCAG CTTCAGCACC TGCCCTTCTC TGTGGAAGAG 
TATGAGATCT CCGCCATGAA TCAGATGTCC TGGCTGATGA AGACTGCGTC CATAGAACTG 
CGCATCACCT CCCTAAACCG CCAACGTTCC CACACGCAGA GGCTCCTTCA CCTGCTGCTT 
GACGACATGC CCACCAAACC GTATTCAGCA GCTGATGGCG AAGGAGGAAT GGAGGACGAA 
AGCCGCAGCC TCAGTGGATT TCTGCACTTT GACACTACCT CAAAAGTCCG CCGGAAAATC 
CTCCGCATCC TGGACTCCAT ACATTTCAGC AATGAGATCC CGGATCCCCT GCAGTTGGAT 
TTCTTCGATC GCAGCCAGAT AGAGCAGGTT ATTGCTAACT GCGAGCACAA GAACCGCAGA 
GGGCAGACCG TCTGCAACGT CAAGCTTCTC CATCGGGTTC TGGTTGCAGA AGTGAATGCC 
CTTCAGGGAA TGGCTGCTAT TGGTCAGAGG CCGCTGCTTA TGGAGGAAAT CAACACCATC 
CTTCAGTACG TGGTGGAAAG GAACAAGCTG CTGCAGTGCC TCCACGCCAA ACGCCATGCG 
CTGGAATCAT GGCGGCAACT TGTGGAGATC ATACTGACCG CCTGTCCCCA GGACCTCATT 
CCGACTGAGC ACAGACAGCT CATCATACGA GACCTGCTGC AAGATCTGCA TGATAAGATC 
CTCGACGACG ACGCTGCCCA AGAACTAATG CCCATTGTCG CCGGGGCAGT CTTCACCCTC 
ACCACTCATC TCAGCCAATC TGTACGTACC GAGCTGAAAC AGCCGATGGC TGCCGCTGGG 
CTGGGCCAGT CGCAATATGT GCAAATGCTG GACGGCTCAT TCGCTGCCCC TCCTGGGACA 
GAAAACCTCT CCGCTGGTTT TGCATCCATT GGAGACTCCT CCCTTCACAT GATTCTCAGG 
AATCTGCTCG AGTTCATTCT AAAAACAGGA GGTGGGTTCC AACGGGTCAG GGCCCACCTG 
TACGGATCGC TGCTGTATTA CCTGCAGATA GCTCAGAGAC CAGATGAACC AGATACTCTG 
GAATCCGCAC ACAAGACTAT GTGGGAGCGA CTGACGGCCC CGGAGGATGT ATTCAGCAAG 
CTGCAGCGAG ACAACCTGTC CATATTCGAG AGCTACGGCA CCGCCCTTAT GGAAGTGGTT 
TGCCGGGACG CCTGTGACGG ACATGACATT GGGAGGATGC TGGCCCTGGC ACTGCTTGAC 
CGGATTGTCT CTGTGGATAG GCAACAGTGG CTGCTTTATC TCTCCAACAG TGGCTACCTG 
AAGGTCCTTG TGGATAGCCT GGCAGAGGAC GATATGGTCC TAAGGAACCT GCTAACTCCT 
CAGGCGCCTC TTCTAAAGGC TCTCTATATC TATGAGTCGA AAATGGCATT TCTGACCCGG 
GTGGCTAAAA GCCAGCAGGG AGCCCTGGAG CTGCTGAGGA GTGGGGTTAT CGTCAGGCTG 
GCACAGTGCC AAGTATATGA TATGCGCCCG GAGACTGACC CCCATGGAGT GTTTGGCATG 
AGAGAGACCC CGGTGTTTAT CCCCGCCCCG GTGGAACGAT ACCGGCAAAT CCTGTTACCC 
GCTCTCCAGA TCTGTCAGCT CATCCTGACC TCCAGCACGG CACAGCACTT ACAGGCCGCC 
GGACAGGTCT TGCAGTTCCT GATCGCTCAT TCTGATACCA TCCAGGCAAT TCTCCGGAGC 
CAGGAGGGGA GTCTGGGTTC CTTGCAGGAG TTGGCTTTGC TTACAGGCAT CATCAGCAAG 
GCCGCACTAC CCGGTGTTCT CAACGAGCTC GATATCGGTT TAAACGAAGG GTCCATGATG 
GAGTTACAAG GACATATTGG CCGCTTCCAG CGCCAGTGTC TTGCGTTATT AAACCGCTTT 
GGGGGGTCCG ACCGACTTCG GCAGCTCAGT CTGCAGGATG ATTCCTCCCA GCCGGACGGT 
GTGAGCAAGA GGGACGACAT GGAGTTGGCC ATGCAACAGA TCTGCTCCAA CGTGATGGAG 
TACTGCCAGA CGCTTATGAT ACAGAACTCG CCAAGCTTCC AGCAGACTGT GTGTCTCTTC 
ACCCCCAACC TCACGGAGAG CTCCAGCCGA GATGGAACAC GGCAGGATTC CCAGGTGTCC 
ATTCTGCCGT CATGGCGGTT GCCAGGCTTG GGTATAGTGA TCCACCTTCT GAAGCAGAGC 
GCCAATAACT TCTTCACTTA CTATGATATC CACCGGCAGA GCGTCAGCAA GCTGCAGAAC 
GTGGAGCAAC TCCCGCCCGA TGAGATTAAA GAGCTGTGCC AGTCTGAGAT GCCGGTTGGC 
GTAGATAAAA TCTCGACGAC GCAGAAGTAC GCGCTGGCAC GGCGGCGACT CGTGAAGCTT 
ATCAACAGCA GAGCCAAACT GCTTTCTCTT TGTAGTTATA TCATAGAAAC GTGTCTGTTC 
ATTCTGTGGC GCCACTTGGA ACATTACCTT CTGCACTGCA CCACCAGCGA CTCCCAGGAC 
CCCGTCTTCT CGAATACGAC CTTCGGCAAC AGAAGGTTTC AAGATACAAT TAACACTGAT 
CCTAACATGG ACCTGAGGAA CCTGAGACAG AATAAAGTCA GCCAACAGGA AATTGATATG 
CTGCTAAGAG AAGGCGCCAA TAGTTTCGGG GAGTCCCTGC AGAAGAGACT GTTAGACATC 
GAATCATTGT ACTGCAAGGT GCGATCCCGT CACAGCTTCA TCCAGGCGCT GGTCAGGCGG 
ATCCGGGGCC TCCTGCGAGT ATCAAGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814138.1
CDS	232..6264
Translation	MAAQLALNSEASLWGPYREIWQTVVGALVKRQPEAIHSLDIVLKKYKPDFISLFKNPPKSAQQHERVQKASSEGIPIKGTQRTRILEGQLIKEAFILSDLYNIGEVAAVELLLLGEQQQPTFHGLTRGLVAILLYWDGKSCMADSLLHLIQARKGRTFTLNHSPELVSMVTRFTDDLMEQGLTNKILTLISQIDVNNEFDKLLKDRGLGNEKHRKEVSDLIKECRQSLAHSLYSWSCQTPLNKEDTLLLIGYLEKVTVEGDGSLDKVNLTLLMSLLYCLDVGFLEQATDDREELIKQTSMFMDRQYIAAIHNRLHDTQPWKLPGMQATVRLAWALALRGISQFSEVLEFSEADEPMAELAISGNVFLFLTEAVVGSESFCADEFFIRRIHKLVTDFLTLMPMKVKQLRNRAEEDARLIHMSMQMGNEPPASLRRDLEHLLLLIGELYRIDPFHLELALEYWCPAEPLQSTSLMGSFLGVAHQRPPQRQVVLSKFVRQMSDLLPATLYIPYLKMLRGLASGPQCAHYCFSLLKANGGSSESLQAAGGSPVSWDHFFHSLMLYHEHLRRDLPSTDSIHLRHPPLRGITQRELDGLIACLQLTLTIVDWSESARLALCEHAQWMPVVVILGLLQCSIPPLLKAELLKTLAAFGKSPEIAASLWQSLEYTQILQTVRTPGLRQGVGIEVELNEIESRCEEYPLTRAFCQLISTLVESSFPTNLGAGLRAPGFEPYLQFLRDTVFLRFRTRAYRRGAEKWEVAEAVLDVFYKLLKDYEPQPEDFVDQYVELQGEERVAFKPPGFSLMHHLLNDSPMLELCLSLMEEGVTQLDTYAPFPGKKHLEKAVAYCFMLLNQTLQKENRFMDLLRESHLSLIVTPLEQLLQGINPRSKKADNVINIARYLYHGNSNPELAFESAKILCSIACNSKIQEKIVGDFTQDQNVTQKLMVGFVSCLDSEEAEELLDSEKEVEDQAKQTNIRYMTKIHILNLLITSLEMKAPNLAMFLLGYELKKPVSTTNLQDSGVLGCPRTCLHSILDILRKGTDGRAGPIAIWDTPHLAELCYQVIYQLCACSDTSGPTMRYLRTSQDFLFTQLQHLPFSVEEYEISAMNQMSWLMKTASIELRITSLNRQRSHTQRLLHLLLDDMPTKPYSAADGEGGMEDESRSLSGFLHFDTTSKVRRKILRILDSIHFSNEIPDPLQLDFFDRSQIEQVIANCEHKNRRGQTVCNVKLLHRVLVAEVNALQGMAAIGQRPLLMEEINTILQYVVERNKLLQCLHAKRHALESWRQLVEIILTACPQDLIPTEHRQLIIRDLLQDLHDKILDDDAAQELMPIVAGAVFTLTTHLSQSVRTELKQPMAAAGLGQSQYVQMLDGSFAAPPGTENLSAGFASIGDSSLHMILRNLLEFILKTGGGFQRVRAHLYGSLLYYLQIAQRPDEPDTLESAHKTMWERLTAPEDVFSKLQRDNLSIFESYGTALMEVVCRDACDGHDIGRMLALALLDRIVSVDRQQWLLYLSNSGYLKVLVDSLAEDDMVLRNLLTPQAPLLKALYIYESKMAFLTRVAKSQQGALELLRSGVIVRLAQCQVYDMRPETDPHGVFGMRETPVFIPAPVERYRQILLPALQICQLILTSSTAQHLQAAGQVLQFLIAHSDTIQAILRSQEGSLGSLQELALLTGIISKAALPGVLNELDIGLNEGSMMELQGHIGRFQRQCLALLNRFGGSDRLRQLSLQDDSSQPDGVSKRDDMELAMQQICSNVMEYCQTLMIQNSPSFQQTVCLFTPNLTESSSRDGTRQDSQVSILPSWRLPGLGIVIHLLKQSANNFFTYYDIHRQSVSKLQNVEQLPPDEIKELCQSEMPVGVDKISTTQKYALARRRLVKLINSRAKLLSLCSYIIETCLFILWRHLEHYLLHCTTSDSQDPVFSNTTFGNRRFQDTINTDPNMDLRNLRQNKVSQQEIDMLLREGANSFGESLQKRLLDIESLYCKVRSRHSFIQALVRRIRGLLRVSRA

Target ORF information:

RefSeq Version	XM_012958684.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nucleoporin 205kDa (nup205), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958684.2

ORF Insert Sequence:

ATGGCGGCGC AGTTGGCGCT AAATTCAGAA GCCAGCCTGT GGGGCCCTTA TAGAGAAATA 
TGGCAGACCG TGGTGGGCGC GCTTGTGAAG AGGCAACCGG AAGCCATTCA TTCGCTCGAT 
ATCGTCTTAA AGAAATATAA ACCGGACTTC ATCTCTCTCT TCAAAAATCC ACCAAAGAGC 
GCTCAGCAAC ATGAAAGAGT GCAGAAAGCC AGCAGCGAGG GGATTCCTAT TAAAGGGACG 
CAGCGGACCC GGATCCTGGA AGGACAGCTC ATTAAGGAAG CCTTCATCCT TAGCGACCTG 
TATAATATCG GAGAGGTGGC AGCCGTAGAA CTTCTACTTT TAGGTGAGCA GCAGCAGCCT 
ACCTTCCATG GCCTGACGAG GGGCTTGGTA GCCATACTGT TGTACTGGGA TGGAAAGAGC 
TGCATGGCTG ATTCCTTATT ACACCTCATC CAGGCAAGAA AAGGCAGGAC CTTCACCTTG 
AATCACAGCC CCGAGCTTGT GTCCATGGTA ACTCGTTTCA CCGATGATCT AATGGAGCAA 
GGCTTGACTA ATAAAATCCT CACCTTGATC TCTCAAATTG ACGTGAACAA TGAGTTTGAC 
AAGTTGCTGA AGGATCGGGG CCTGGGCAAC GAGAAGCATC GGAAAGAGGT TTCTGATCTG 
ATAAAGGAAT GTCGGCAGTC GCTCGCACAC AGCCTGTATT CATGGAGTTG CCAGACACCA 
CTAAACAAAG AAGATACACT ACTTTTGATT GGATACTTGG AAAAAGTAAC AGTAGAAGGA 
GATGGCTCAT TGGACAAAGT GAACTTGACC CTACTGATGT CACTTCTCTA TTGCTTGGAT 
GTTGGCTTTT TGGAGCAAGC GACAGATGAT CGAGAAGAAC TGATAAAGCA GACCAGCATG 
TTCATGGACA GACAGTACAT TGCCGCCATC CATAACCGCC TGCACGACAC TCAGCCGTGG 
AAGCTGCCGG GAATGCAGGC TACGGTCAGA CTCGCTTGGG CCCTGGCTCT TCGAGGGATC 
TCTCAGTTCT CAGAAGTCTT AGAATTTTCA GAAGCAGACG AGCCAATGGC AGAGCTAGCG 
ATCAGCGGCA ATGTCTTCCT CTTCCTCACG GAGGCAGTGG TGGGATCCGA AAGCTTCTGT 
GCAGATGAGT TCTTTATACG AAGGATTCAC AAACTCGTCA CCGATTTCCT TACTCTCATG 
CCCATGAAAG TAAAGCAGCT GAGAAATCGT GCAGAAGAGG ATGCCCGACT GATCCATATG 
AGCATGCAGA TGGGAAATGA GCCCCCTGCT TCTCTGCGTA GGGATTTGGA ACATCTGCTC 
TTACTGATCG GTGAGCTCTA CCGGATAGAC CCATTCCACT TGGAACTGGC GTTGGAATAC 
TGGTGCCCCG CAGAGCCTCT CCAGTCGACA TCCTTGATGG GATCCTTTCT TGGTGTGGCC 
CACCAGAGAC CACCTCAACG TCAGGTCGTC TTGTCCAAGT TCGTGAGACA GATGAGTGAC 
CTGCTGCCAG CCACGCTCTA CATACCTTAT CTTAAGATGC TCAGGGGCCT TGCAAGTGGC 
CCTCAGTGCG CCCATTACTG TTTCAGTCTG CTAAAGGCCA ATGGTGGCAG CAGCGAGAGC 
TTGCAAGCTG CAGGGGGTAG CCCAGTGTCC TGGGACCACT TCTTCCACTC TTTGATGCTG 
TACCACGAGC ACTTGCGGAG GGATCTACCA AGCACAGACA GTATACACCT GCGTCACCCT 
CCTCTCCGTG GTATCACCCA GCGAGAGCTC GATGGCCTGA TTGCCTGCTT GCAGCTCACC 
CTTACCATCG TCGATTGGAG TGAAAGTGCC CGCCTTGCTC TGTGTGAGCA TGCCCAGTGG 
ATGCCAGTGG TCGTTATTCT TGGCCTCCTG CAGTGCAGCA TCCCACCACT CCTTAAGGCT 
GAGCTCCTGA AGACACTGGC TGCATTTGGC AAATCCCCAG AGATCGCAGC ATCCCTCTGG 
CAATCCCTCG AATATACACA GATTTTGCAG ACTGTAAGAA CCCCAGGGCT ACGGCAAGGA 
GTCGGGATTG AGGTGGAACT GAATGAGATC GAGTCTCGGT GTGAGGAGTA CCCCCTGACC 
AGAGCATTCT GCCAGCTCAT TAGCACTTTA GTGGAGAGCT CCTTCCCCAC CAATCTGGGA 
GCGGGTTTGA GAGCACCCGG CTTTGAGCCA TACCTCCAGT TTCTACGGGA CACTGTGTTC 
CTTCGGTTCC GCACTAGAGC CTACAGGAGA GGCGCAGAAA AGTGGGAGGT AGCAGAGGCT 
GTACTGGATG TCTTCTACAA GCTTTTGAAG GACTATGAGC CCCAGCCTGA AGACTTTGTG 
GACCAGTATG TGGAACTCCA AGGAGAAGAA AGAGTTGCTT TCAAGCCCCC GGGGTTCAGC 
CTCATGCATC ACCTTCTCAA TGATTCTCCG ATGCTGGAGC TCTGCCTCAG CCTCATGGAA 
GAGGGGGTCA CCCAGCTGGA CACTTACGCC CCTTTTCCAG GCAAGAAACA CCTAGAGAAA 
GCAGTGGCTT ATTGTTTCAT GCTCCTCAAT CAGACTCTGC AGAAGGAGAA TCGCTTCATG 
GATTTGTTGA GGGAAAGTCA CCTGTCGTTG ATTGTGACCC CTCTGGAGCA GCTCCTGCAG 
GGAATTAACC CACGATCTAA GAAAGCCGAT AATGTCATCA ATATTGCTCG ATATCTCTAC 
CATGGGAACA GCAATCCCGA GCTTGCCTTT GAAAGTGCAA AGATCTTATG CAGCATCGCC 
TGTAACTCCA AGATCCAAGA AAAGATTGTA GGAGACTTTA CCCAAGATCA GAACGTGACC 
CAGAAGCTGA TGGTTGGGTT TGTTTCATGC CTGGACAGCG AGGAAGCAGA GGAGTTACTG 
GATTCAGAGA AAGAGGTGGA GGATCAAGCC AAGCAGACGA ACATCCGGTA CATGACAAAG 
ATTCACATCC TCAATCTTCT GATCACCTCC CTGGAAATGA AAGCCCCCAA CCTTGCCATG 
TTCCTTCTCG GCTACGAACT GAAGAAGCCA GTCAGCACGA CCAACCTGCA GGACTCTGGT 
GTCCTGGGCT GCCCGCGGAC CTGCCTTCAC TCCATTTTAG ACATCTTGAG AAAGGGGACT 
GATGGGCGGG CTGGACCCAT AGCCATATGG GATACTCCTC ATCTTGCTGA ACTGTGTTAC 
CAGGTGATCT ACCAGCTGTG CGCTTGTTCC GATACCTCTG GCCCCACCAT GAGATACCTA 
CGGACCAGCC AGGATTTCCT CTTCACTCAG CTTCAGCACC TGCCCTTCTC TGTGGAAGAG 
TATGAGATCT CCGCCATGAA TCAGATGTCC TGGCTGATGA AGACTGCGTC CATAGAACTG 
CGCATCACCT CCCTAAACCG CCAACGTTCC CACACGCAGA GGCTCCTTCA CCTGCTGCTT 
GACGACATGC CCACCAAACC GTATTCAGCA GCTGATGGCG AAGGAGGAAT GGAGGACGAA 
AGCCGCAGCC TCAGTGGATT TCTGCACTTT GACACTACCT CAAAAGTCCG CCGGAAAATC 
CTCCGCATCC TGGACTCCAT ACATTTCAGC AATGAGATCC CGGATCCCCT GCAGTTGGAT 
TTCTTCGATC GCAGCCAGAT AGAGCAGGTT ATTGCTAACT GCGAGCACAA GAACCGCAGA 
GGGCAGACCG TCTGCAACGT CAAGCTTCTC CATCGGGTTC TGGTTGCAGA AGTGAATGCC 
CTTCAGGGAA TGGCTGCTAT TGGTCAGAGG CCGCTGCTTA TGGAGGAAAT CAACACCATC 
CTTCAGTACG TGGTGGAAAG GAACAAGCTG CTGCAGTGCC TCCACGCCAA ACGCCATGCG 
CTGGAATCAT GGCGGCAACT TGTGGAGATC ATACTGACCG CCTGTCCCCA GGACCTCATT 
CCGACTGAGC ACAGACAGCT CATCATACGA GACCTGCTGC AAGATCTGCA TGATAAGATC 
CTCGACGACG ACGCTGCCCA AGAACTAATG CCCATTGTCG CCGGGGCAGT CTTCACCCTC 
ACCACTCATC TCAGCCAATC TGTACGTACC GAGCTGAAAC AGCCGATGGC TGCCGCTGGG 
CTGGGCCAGT CGCAATATGT GCAAATGCTG GACGGCTCAT TCGCTGCCCC TCCTGGGACA 
GAAAACCTCT CCGCTGGTTT TGCATCCATT GGAGACTCCT CCCTTCACAT GATTCTCAGG 
AATCTGCTCG AGTTCATTCT AAAAACAGGA GGTGGGTTCC AACGGGTCAG GGCCCACCTG 
TACGGATCGC TGCTGTATTA CCTGCAGATA GCTCAGAGAC CAGATGAACC AGATACTCTG 
GAATCCGCAC ACAAGACTAT GTGGGAGCGA CTGACGGCCC CGGAGGATGT ATTCAGCAAG 
CTGCAGCGAG ACAACCTGTC CATATTCGAG AGCTACGGCA CCGCCCTTAT GGAAGTGGTT 
TGCCGGGACG CCTGTGACGG ACATGACATT GGGAGGATGC TGGCCCTGGC ACTGCTTGAC 
CGGATTGTCT CTGTGGATAG GCAACAGTGG CTGCTTTATC TCTCCAACAG TGGCTACCTG 
AAGGTCCTTG TGGATAGCCT GGCAGAGGAC GATATGGTCC TAAGGAACCT GCTAACTCCT 
CAGGCGCCTC TTCTAAAGGC TCTCTATATC TATGAGTCGA AAATGGCATT TCTGACCCGG 
GTGGCTAAAA GCCAGCAGGG AGCCCTGGAG CTGCTGAGGA GTGGGGTTAT CGTCAGGCTG 
GCACAGTGCC AAGTATATGA TATGCGCCCG GAGACTGACC CCCATGGAGT GTTTGGCATG 
AGAGAGACCC CGGTGTTTAT CCCCGCCCCG GTGGAACGAT ACCGGCAAAT CCTGTTACCC 
GCTCTCCAGA TCTGTCAGCT CATCCTGACC TCCAGCACGG CACAGCACTT ACAGGCCGCC 
GGACAGGTCT TGCAGTTCCT GATCGCTCAT TCTGATACCA TCCAGGCAAT TCTCCGGAGC 
CAGGAGGGGA GTCTGGGTTC CTTGCAGGAG TTGGCTTTGC TTACAGGCAT CATCAGCAAG 
GCCGCACTAC CCGGTGTTCT CAACGAGCTC GATATCGGTT TAAACGAAGG GTCCATGATG 
GAGTTACAAG GACATATTGG CCGCTTCCAG CGCCAGTGTC TTGCGTTATT AAACCGCTTT 
GGGGGGTCCG ACCGACTTCG GCAGCTCAGT CTGCAGGATG ATTCCTCCCA GCCGGACGGT 
GTGAGCAAGA GGGACGACAT GGAGTTGGCC ATGCAACAGA TCTGCTCCAA CGTGATGGAG 
TACTGCCAGA CGCTTATGAT ACAGAACTCG CCAAGCTTCC AGCAGACTGT GTGTCTCTTC 
ACCCCCAACC TCACGGAGAG CTCCAGCCGA GATGGAACAC GGCAGGATTC CCAGGTGTCC 
ATTCTGCCGT CATGGCGGTT GCCAGGCTTG GGTATAGTGA TCCACCTTCT GAAGCAGAGC 
GCCAATAACT TCTTCACTTA CTATGATATC CACCGGCAGA GCGTCAGCAA GCTGCAGAAC 
GTGGAGCAAC TCCCGCCCGA TGAGATTAAA GAGCTGTGCC AGTCTGAGAT GCCGGTTGGC 
GTAGATAAAA TCTCGACGAC GCAGAAGTAC GCGCTGGCAC GGCGGCGACT CGTGAAGCTT 
ATCAACAGCA GAGCCAAACT GCTTTCTCTT TGTAGTTATA TCATAGAAAC GTGTCTGTTC 
ATTCTGTGGC GCCACTTGGA ACATTACCTT CTGCACTGCA CCACCAGCGA CTCCCAGGAC 
CCCGTCTTCT CGAATACGAC CTTCGGCAAC AGAAGGTTTC AAGATACAAT TAACACTGAT 
CCTAACATGG ACCTGAGGAA CCTGAGACAG AATAAAGTCA GCCAACAGGA AATTGATATG 
CTGCTAAGAG AAGGCGCCAA TAGTTTCGGG GAGTCCCTGC AGAAGAGACT GTTAGACATC 
GAATCATTGT ACTGCAAGGT GCGATCCCGT CACAGCTTCA TCCAGGCGCT GGTCAGGCGG 
ATCCGGGGCC TCCTGCGAGT ATCAAGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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nup205 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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