hectd1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	hectd1
Entrez Gene ID	100192372
Full Name	HECT domain containing E3 ubiquitin protein ligase 1
General protein information	Preferred Names HECT domain containing E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase HECTD1 HECT domain containing 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012968096.2	XP_012823550.1	E3 ubiquitin-protein ligase HECTD1 isoform X1
XM_012968095.2	XP_012823549.1	E3 ubiquitin-protein ligase HECTD1 isoform X1
XM_012968097.2	XP_012823551.1	E3 ubiquitin-protein ligase HECTD1 isoform X2
XM_012968097.3	XP_012823551.1	E3 ubiquitin-protein ligase HECTD1 isoform X2
NM_001137613.1	NP_001131085.1	E3 ubiquitin-protein ligase HECTD1
XM_012968095.3	XP_012823549.1	E3 ubiquitin-protein ligase HECTD1 isoform X1
XM_012968096.3	XP_012823550.1	E3 ubiquitin-protein ligase HECTD1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HECTD1	NP_056197.2
Pan troglodytes (chimpanzee)	HECTD1	XP_509889.2
Drosophila melanogaster (fruit fly)	CG5604	NP_609369.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009511	XP_310184.4
Mus musculus (house mouse)	Hectd1	NP_659037.2
Canis lupus familiaris (dog)	HECTD1	XP_850301.2
Caenorhabditis elegans (roundworm)	hecd-1	NP_501120.1
Danio rerio (zebrafish)	hectd1	NP_001002504.2
	HECTD1	XP_001115000.1
Bos taurus (cattle)	HECTD1	XP_005222128.1
Rattus norvegicus (Norway rat)	Hectd1	XP_006240148.1
Gallus gallus (chicken)	HECTD1	XP_421227.3
Xenopus tropicalis (tropical clawed frog)	hectd1	NP_001131085.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hectd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hectd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa63529	XM_012968096.3 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa63529	XM_012968095.3 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa63530	XM_012968097.3 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa22713	NM_001137613.1 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa32491	XM_012968096.2 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa32491	XM_012968095.2 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa32492	XM_012968097.2 Latest version!	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hectd1 ( XM_012968095.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823549.1 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63529
Clone ID Related Accession (Same CDS sequence)	XM_012968095.3 , XM_012968096.3
Accession Version	XM_012968095.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA 
GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG 
GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC 
TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC 
CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC 
TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA 
TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC 
TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG 
GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG 
CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG 
AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA 
AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG 
ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG 
TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT 
GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG 
CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG 
CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA 
AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG 
CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT 
AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT 
CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA 
ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG 
CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG 
TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA 
CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC 
CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT 
GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA 
TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG 
ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA 
TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG 
ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT 
GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC 
AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA 
GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA 
GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT 
GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT 
TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG 
CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA 
TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC 
TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG 
AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC 
CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA 
GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT 
GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA 
TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT 
TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA 
AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC 
AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA 
CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACCGTTACA 
ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA 
GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC 
ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT 
TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA 
AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT 
GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT 
TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT 
GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT 
GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG CGGGAGAGAG 
GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG 
CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC 
TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG 
GGCTGTTGGT CTGTAGAGCA TGTAGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT 
GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG 
ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC 
AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC 
CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC 
CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC 
AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT 
GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG 
GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA 
TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT 
GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA 
ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC 
GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA 
ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC 
TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG 
GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT 
GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC 
TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT 
TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT 
TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT 
CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG 
CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC 
CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG 
CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG 
AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA 
CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC 
AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA 
AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT 
GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT 
AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG 
TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG 
CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG 
TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA 
GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT 
GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC 
GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823549.1
CDS	305..8047
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVEGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDASLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAQHGLTEELLSRMAAAGGTVSGPSSACKTGRGTSGGPSTSGDSKISNQVSTIVSLLSTLCRGSPVVTHDLLRAELLDSMESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDEKKKKDSNREEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCHSDAGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGDDLFLDQLARLGVISKVSTLAGPTSDDENEEDSKPEKVRIHLFPLYSAHKGTQAAASQLKEDEPQEDAKELQQGRPYHWRDWSVIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPSTSSQPILSTPGPGKLTVGNWSLTCLKDGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESAWELHTNRQCIEGENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNNEDCDIKQDCGQLVERLNVFKTAFSENEDDESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETSLIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQSCVFRHQHDFDDNGIMYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKSAWFAIDLGLWVVPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTTLYTHMDDCSLNEPGSTATWPLDPAREEKQGWRHVRIKQTGKNASGQTHYLSLSGFELYGNVTGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGIDWKWRDQDGSAQGEGTVTGELHNGWIDVIWDAGGSNSYRMGAEGKFDLKLAPGYDPESAVSPKPVSSTVAGTPPSWSSLVKNNCPDKAPPSSSSSCVVVGSVAGSGSRKGSSSSVCSVASSSDVSLSCAKTERRAEEQVSDIHHDPILLLSSNQAASGSSTCPPGGETVGEGGDRKAGEAPAISMGMVSISSPDVSSVSELSNKEVAVPRPLGSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNAVSSATSPLMGAQSFPNLTTTGTTSTVTMSTSSVTSSNVATATTGLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEEQEYEEVMEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPAPGTPHSELLEEVECAPAPRLALTLKVTGLGSGREVELPLNNFRSTIFFYVQRLLQLSCNGAIKTDKLRRIWEPTYTIMYREMKDSDKQKECGRLGCWSVEHVEQSLGTDALPKNDLITYLQRNADPGFLRRWKLTGTNKSIRKNRNCSQLIAAYKDFCENGCKSLSMPAALATLQSADILSHSREQAQAKAGSSQNSCGVEDVLQLLRILFIVASDPYSARTPQEDGEDMLLFSVPPEEFTSKKITTKIVQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERSRTASAVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQKTDLGIWLCDDDFPDDESRQVDLGGGLKPPGYYVQRSCGLFIAPYPQDSEELDRVTRLCHFLGVFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLLYASRGEESEHCTESQSEASTEDGHDALSVGSFEEDCKSEFILDPPKPKPPAWFQGILTWEDFELINPHRARFLRDIRELAVKRRQILGNRCLSEDEKNTQLQELMLKNPSGSGPPVSIEDLGLNFQFCPSSRVYGFSAVDLRPNGEDEMVTIDNAEEYVDLMFDFCMQTGVQKQMEAFRSGFNKVFPMEKLGSFSPEEVQMILCGNQSPSWSAEDIINYTEPKLGYTRESPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRDRLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_012968095.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968095.3

ORF Insert Sequence:

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA 
GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG 
GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC 
TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC 
CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC 
TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA 
TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC 
TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG 
GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG 
CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG 
AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA 
AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG 
ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG 
TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT 
GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG 
CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG 
CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA 
AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG 
CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT 
AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT 
CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA 
ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG 
CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG 
TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA 
CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC 
CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT 
GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA 
TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG 
ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA 
TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG 
ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT 
GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC 
AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA 
GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA 
GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT 
GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT 
TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG 
CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA 
TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC 
TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG 
AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC 
CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA 
GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT 
GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA 
TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT 
TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA 
AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC 
AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA 
CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACCGTTACA 
ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA 
GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC 
ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT 
TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA 
AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT 
GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT 
TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT 
GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT 
GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG CGGGAGAGAG 
GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG 
CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC 
TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG 
GGCTGTTGGT CTGTAGAGCA TGTAGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT 
GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG 
ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC 
AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC 
CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC 
CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC 
AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT 
GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG 
GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA 
TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT 
GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA 
ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC 
GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA 
ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC 
TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG 
GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT 
GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC 
TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT 
TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT 
TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT 
CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG 
CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC 
CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG 
CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG 
AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA 
CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC 
AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA 
AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT 
GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT 
AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG 
TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG 
CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG 
TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA 
GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT 
GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC 
GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd1 ( XM_012968096.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823550.1 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.

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CloneID	OXa63529
Clone ID Related Accession (Same CDS sequence)	XM_012968095.3 , XM_012968096.3
Accession Version	XM_012968096.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA 
GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG 
GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC 
TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC 
CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC 
TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA 
TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC 
TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG 
GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG 
CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG 
AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA 
AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG 
ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG 
TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT 
GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG 
CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG 
CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA 
AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG 
CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT 
AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT 
CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA 
ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG 
CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG 
TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA 
CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC 
CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT 
GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA 
TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG 
ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA 
TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG 
ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT 
GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC 
AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA 
GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA 
GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT 
GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT 
TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG 
CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA 
TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC 
TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG 
AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC 
CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA 
GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT 
GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA 
TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT 
TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA 
AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC 
AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA 
CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACCGTTACA 
ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA 
GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC 
ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT 
TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA 
AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT 
GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT 
TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT 
GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT 
GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG CGGGAGAGAG 
GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG 
CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC 
TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG 
GGCTGTTGGT CTGTAGAGCA TGTAGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT 
GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG 
ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC 
AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC 
CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC 
CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC 
AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT 
GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG 
GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA 
TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT 
GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA 
ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC 
GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA 
ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC 
TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG 
GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT 
GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC 
TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT 
TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT 
TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT 
CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG 
CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC 
CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG 
CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG 
AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA 
CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC 
AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA 
AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT 
GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT 
AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG 
TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG 
CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG 
TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA 
GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT 
GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC 
GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823550.1
CDS	205..7947
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVEGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDASLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAQHGLTEELLSRMAAAGGTVSGPSSACKTGRGTSGGPSTSGDSKISNQVSTIVSLLSTLCRGSPVVTHDLLRAELLDSMESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDEKKKKDSNREEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCHSDAGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGDDLFLDQLARLGVISKVSTLAGPTSDDENEEDSKPEKVRIHLFPLYSAHKGTQAAASQLKEDEPQEDAKELQQGRPYHWRDWSVIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPSTSSQPILSTPGPGKLTVGNWSLTCLKDGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESAWELHTNRQCIEGENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNNEDCDIKQDCGQLVERLNVFKTAFSENEDDESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETSLIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQSCVFRHQHDFDDNGIMYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKSAWFAIDLGLWVVPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTTLYTHMDDCSLNEPGSTATWPLDPAREEKQGWRHVRIKQTGKNASGQTHYLSLSGFELYGNVTGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGIDWKWRDQDGSAQGEGTVTGELHNGWIDVIWDAGGSNSYRMGAEGKFDLKLAPGYDPESAVSPKPVSSTVAGTPPSWSSLVKNNCPDKAPPSSSSSCVVVGSVAGSGSRKGSSSSVCSVASSSDVSLSCAKTERRAEEQVSDIHHDPILLLSSNQAASGSSTCPPGGETVGEGGDRKAGEAPAISMGMVSISSPDVSSVSELSNKEVAVPRPLGSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNAVSSATSPLMGAQSFPNLTTTGTTSTVTMSTSSVTSSNVATATTGLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEEQEYEEVMEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPAPGTPHSELLEEVECAPAPRLALTLKVTGLGSGREVELPLNNFRSTIFFYVQRLLQLSCNGAIKTDKLRRIWEPTYTIMYREMKDSDKQKECGRLGCWSVEHVEQSLGTDALPKNDLITYLQRNADPGFLRRWKLTGTNKSIRKNRNCSQLIAAYKDFCENGCKSLSMPAALATLQSADILSHSREQAQAKAGSSQNSCGVEDVLQLLRILFIVASDPYSARTPQEDGEDMLLFSVPPEEFTSKKITTKIVQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERSRTASAVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQKTDLGIWLCDDDFPDDESRQVDLGGGLKPPGYYVQRSCGLFIAPYPQDSEELDRVTRLCHFLGVFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLLYASRGEESEHCTESQSEASTEDGHDALSVGSFEEDCKSEFILDPPKPKPPAWFQGILTWEDFELINPHRARFLRDIRELAVKRRQILGNRCLSEDEKNTQLQELMLKNPSGSGPPVSIEDLGLNFQFCPSSRVYGFSAVDLRPNGEDEMVTIDNAEEYVDLMFDFCMQTGVQKQMEAFRSGFNKVFPMEKLGSFSPEEVQMILCGNQSPSWSAEDIINYTEPKLGYTRESPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRDRLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_012968096.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968096.3

ORF Insert Sequence:

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA 
GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG 
GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC 
TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC 
CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC 
TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA 
TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC 
TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG 
GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG 
CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG 
AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA 
AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG 
ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG 
TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT 
GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG 
CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG 
CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA 
AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG 
CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT 
AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT 
CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA 
ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG 
CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG 
TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA 
CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC 
CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT 
GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA 
TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG 
ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA 
TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG 
ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT 
GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC 
AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA 
GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA 
GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT 
GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT 
TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG 
CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA 
TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC 
TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG 
AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC 
CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA 
GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT 
GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA 
TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT 
TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA 
AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC 
AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA 
CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACCGTTACA 
ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA 
GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC 
ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT 
TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA 
AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT 
GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT 
TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT 
GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT 
GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG CGGGAGAGAG 
GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG 
CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC 
TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG 
GGCTGTTGGT CTGTAGAGCA TGTAGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT 
GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG 
ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC 
AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC 
CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC 
CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC 
AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT 
GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG 
GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA 
TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT 
GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA 
ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC 
GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA 
ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC 
TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG 
GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT 
GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC 
TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT 
TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT 
TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT 
CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG 
CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC 
CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG 
CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG 
AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA 
CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC 
AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA 
AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT 
GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT 
AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG 
TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG 
CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG 
TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA 
GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT 
GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC 
GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd1 ( XM_012968097.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823551.1 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.

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CloneID	OXa63530
Clone ID Related Accession (Same CDS sequence)	XM_012968097.3
Accession Version	XM_012968097.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGAGGATGA GCCACAGGAA GATGCCAAGG AGTTGCAACA GGGCCGGCCC 
TACCATTGGC GGGACTGGTC TGTTATTCGC GGTAGGGACT GTTTGTACAT CTGGAGTGAT 
GCTGCAGCTC TGGAGCTCTC TAATGGTAGC AATGGCTGGT TCCGCTTTAT CCTAGATGGG 
AAACTGGCAA CCATGTATTC AAGTGGAAGC CCAGAGGGGG GTTCTGATAG CTCAGAAAGT 
AGAAGTGAAT TTTTAGAAAA GTTACAGCGG GCCCGCAGCC AGGTCAAACC ATCCACTTCT 
AGCCAGCCTA TCTTGTCCAC TCCTGGTCCA GGAAAGCTAA CAGTAGGCAA CTGGTCTCTC 
ACTTGTCTGA AAGATGGTGA GATTGCAATC CACAACTCGG ACGGGCAGCA GGCTACAATA 
CTGAAGGAGG ATCTACCAGG CTTTGTGTTT GAGTCAAACA GGGGTACCAA GCACTCCTTC 
ACGGCAGAAA CATCACTAGG ATCTGAATTT GTAACTGGCT GGACAGGAAA GAGAGGCAGA 
AAGCTGAAGT CTAAGTTGGA GAAAACTAAA CAGAAGGTAC GGACAATGGC AAGAGATTTG 
TATGATGATC ATTTTAAGGC AGTGGAAAGC ATGCCCCGAG GAGTGGTAGT GACGCTCCGA 
AATATTGCAA CACAGCTGGA ATCTGCTTGG GAACTTCATA CAAACAGACA GTGTATTGAA 
GGAGAGAACA CATGGAGAGA TCTGATGAAA ACTGCCCTGG AAAACCTGAT TGTGCTACTG 
AAGGATGAGA ATACTATTTC CCCTTATGAG ATGTGCAGCA GTGGACTGGT GCAGGCTTTG 
CTTACGGTGC TCAATAATAA TGAAGACTGT GATATAAAAC AAGACTGTGG GCAGCTGGTG 
GAGAGACTTA ATGTATTTAA AACTGCCTTC AGCGAAAATG AAGATGATGA AAGCCGCCCT 
GCCGTTGCCC TCGTCCGGAA GTTGATTGCT GTGCTTGAAT CAATAGAACG GCTGCCCCTT 
CACCTGTATG ACACCCCTGG ATCTAGCTAT AACTTGCAGA TCCTGACCCG TAGGCTTCGG 
TTTCGGCTGG AGCGTGCACC TGGAGAAACG TCTCTAATCG ACCGTACCGG TCGGATGCTG 
AAAATGGAGC CACTGGCAAC TGTAGAGTCC TTGGAGCAGT ATTTACTGAA AATGGTGGCT 
AAGCAGTGGT ACGACTTTGA CCGTTCGTCC TTTGTGTTTG TGCGCAAATT GCGCGAGGGC 
CAGAGTTGTG TGTTCCGCCA CCAGCATGAC TTTGATGATA ATGGAATCAT GTACTGGATT 
GGCACCAATG CAAAGACAGC TTATGAGTGG GTCAACCCTG CTGCTTACGG ACTAGTAGTT 
GTTACATCTT CTGAAGGCCG CAATTTGCCA TATGGCCGCC TTGAGGATAT CCTGAGCCGT 
GATAGCTCTG CCCTTAATTG CCACACTAAT GATGATAAGA GTGCCTGGTT TGCCATTGAC 
CTTGGCTTGT GGGTTGTGCC TTCTGCCTAT ACACTGCGTC ATGCTCGTGG ATATGGCCGT 
TCCGCTCTTC GCAATTGGGT CTTCCAGGTG TCTAAAGATG GTCAGAACTG GACCACCCTC 
TACACCCACA TGGATGACTG CAGCCTGAAT GAACCTGGCT CCACAGCCAC ATGGCCTCTG 
GACCCTGCGA GAGAAGAAAA GCAGGGTTGG CGTCATGTCC GGATAAAGCA GACGGGAAAG 
AATGCCAGTG GCCAGACCCA CTACCTCTCA CTTTCTGGCT TTGAGCTTTA TGGCAATGTG 
ACTGGGGTGT GTGAAGACCA ACTTGGTAAA GCTGCCAAAG AGGCAGAAGC CAACTTAAGG 
CGCCAGAGGC GGCTGGTACG CTCTCAGGTG CTAAAATATA TGGTGCCTGG AGCCCGTGTT 
ATTCGGGGCA TTGATTGGAA GTGGCGAGAC CAAGATGGCA GTGCTCAAGG AGAAGGCACT 
GTTACAGGAG AACTGCACAA TGGCTGGATT GATGTCATAT GGGATGCCGG TGGCTCTAAC 
TCTTACCGCA TGGGCGCGGA AGGAAAATTT GACCTGAAGC TGGCCCCGGG TTATGACCCA 
GAGTCTGCGG TGTCACCCAA ACCTGTTTCA TCCACTGTTG CAGGAACACC GCCCTCGTGG 
AGCAGCCTGG TGAAAAACAA CTGTCCTGAC AAAGCCCCTC CCTCTTCCTC ATCCTCTTGT 
GTGGTCGTTG GAAGCGTGGC GGGCTCAGGC AGCCGCAAAG GAAGCAGCAG CTCGGTCTGT 
AGCGTGGCAA GCAGCAGCGA CGTAAGCCTA AGCTGTGCAA AGACAGAGAG GAGGGCAGAG 
GAGCAAGTCT CCGACATTCA CCATGATCCC ATCCTGCTTC TGTCCTCTAA CCAAGCAGCC 
TCGGGTTCCT CCACCTGCCC CCCTGGAGGA GAGACTGTGG GTGAAGGTGG AGATAGAAAG 
GCAGGAGAAG CACCAGCAAT CTCAATGGGT ATGGTGAGCA TTAGCTCCCC TGATGTCAGT 
TCCGTTTCCG AACTCAGCAA CAAGGAAGTG GCAGTTCCAC GACCACTGGG ATCTTCTGCC 
AGCAACAGAT TGTCTGTCAG TTCACTGTTG GCAGCTGGGG CACCCATGAG TTCTAGCGCC 
AGTGTACCAA ACTTGTCCTC CAGGGAAACG TCCAGTCTGG AGTCCTTTGT AAGGAGAGTG 
GCAAATATCG CCAGGACAAA TGCAACCAAC AACATGAACC TGAGTCGTAG CAGCAGTGAT 
AACAATACCA ACACTCTGGG GAGGAATGCA GTCAGCTCAG CCACTTCACC ACTTATGGGA 
GCTCAGAGCT TTCCCAACCT GACGACAACA GGAACCACAT CCACCGTTAC AATGTCTACA 
TCCAGTGTCA CCAGCAGTAA TGTGGCAACA GCCACTACAG GGTTGTCTGT AGGTCAGTCC 
CTCAGTAACA CCCTGACTAC AAGCCTAACC TCGACATCCA GTGAGAGTGA CACTGGGCAG 
GAGGCAGAAT ACTCCCTGTA TGACTTTCTT GATAGCTGCC GTGCCAGCAC TTTGCTTGCT 
GAACTTGATG ATGACGAGGA TCTCCCTGAG CCCGATGAGG AAGATGATGA AAACGAAGAT 
GATAATCAGG AAGAGCAAGA GTATGAAGAA GTTATGGAGG AGGAAGAGTA TGAGACCAAA 
GGTGGCCGAC GCAGGACCTG GGATGATGAT TATGTGCTGA AGAGGCAGTT TTCTGCTTTG 
GTCCCGGCTT TTGACCCACG CCCAGGGAGA ACTAATGTTC AGCAGACCAC TGATCTTGAA 
ATTCCTGCTC CAGGCACGCC CCACTCTGAG CTACTGGAGG AGGTAGAATG TGCCCCAGCT 
CCACGATTAG CACTCACCCT AAAGGTGACA GGCCTTGGGA GCGGGAGAGA GGTGGAGCTC 
CCCCTGAACA ATTTCCGTTC CACCATATTT TTCTATGTAC AGAGACTGCT GCAACTCTCC 
TGCAATGGAG CCATCAAAAC TGACAAGCTG AGAAGGATTT GGGAGCCCAC CTACACGATT 
ATGTACAGAG AGATGAAGGA TTCAGATAAG CAAAAAGAAT GTGGCAGACT GGGCTGTTGG 
TCTGTAGAGC ATGTAGAACA ATCCCTAGGC ACAGATGCCT TGCCAAAGAA TGACCTGATC 
ACTTACCTGC AGCGAAATGC AGATCCAGGG TTCTTGCGAC GCTGGAAACT GACAGGAACC 
AACAAAAGCA TTAGGAAAAA CCGCAACTGT TCCCAGCTTA TTGCTGCTTA CAAGGACTTC 
TGTGAGAATG GCTGCAAGTC TCTCTCAATG CCAGCTGCTC TTGCCACACT CCAGAGTGCT 
GATATCCTTA GTCATTCCCG AGAGCAGGCA CAAGCCAAAG CGGGCAGCAG CCAAAACTCC 
TGCGGGGTAG AAGATGTTTT GCAGCTCCTG CGAATTCTCT TTATTGTAGC CAGTGACCCA 
TATTCTGCCA GGACCCCACA GGAAGATGGA GAAGATATGC TCCTTTTCAG TGTTCCTCCA 
GAGGAGTTTA CTAGCAAGAA AATCACCACA AAGATTGTTC AGCAAATCGA GGAACCTTTA 
GCTTTAGCAA GTGGAGCTTT GCCAGACTGG TGTGAACAGC TGACCAGCAA ATGTCCCTTC 
TTAATACCCT TTGAAACCCG GCAGCTTTAC TTCACATGCA CAGCATTTGG TGCCTCAAGG 
GCTATTGTGT GGCTGCAGAA CAGGCGGGAA GCCACAGTTG AGAGGAGCAG AACAGCAAGT 
GCAGTGCGCA GAGACGACCC AGGGGAGTTC AGAGTGGGCC GTCTAAAACA CGAACGTGTG 
AAGGTCCCAC GAGGGGAGTC TTTGATGGAG TGGGCAGAGA ATGTTATGCA AATCCACGCA 
GACCGAAAAT CTGTGCTTGA GGTGGAGTTT CTGGGAGAAG AAGGAACAGG CTTGGGCCCT 
ACCCTTGAGT TTTATGCCCT GGTGGCAGCA GAGTTCCAGA AAACAGATTT GGGGATCTGG 
TTATGTGATG ATGACTTCCC AGATGATGAG TCTCGGCAGG TGGATCTAGG TGGTGGTTTG 
AAGCCCCCTG GTTACTATGT GCAGCGTTCC TGTGGCCTCT TCATTGCACC CTACCCACAG 
GATAGTGAGG AACTAGACCG AGTGACCAGA CTTTGCCATT TCCTTGGTGT TTTCCTTGCA 
AAGTGCATCC AGGACAATAG ATTAGTGGAC TTGCCCATTT CAAAGCCGTT TTTTAAGCTT 
ATGTGTATGG GTGACATTAA GAGCAACATG AGCAAGCTTT TGTATGCCTC TCGGGGAGAG 
GAGAGTGAGC ACTGCACGGA GAGCCAGTCG GAGGCCTCTA CTGAGGATGG GCATGATGCC 
CTTTCAGTAG GAAGCTTTGA GGAAGACTGC AAATCTGAGT TCATCCTAGA CCCACCCAAA 
CCAAAGCCAC CAGCCTGGTT TCAGGGTATT TTAACTTGGG AGGACTTTGA GCTAATAAAC 
CCACATCGTG CTCGCTTCCT TAGGGACATT AGGGAGTTGG CTGTAAAGAG GAGACAGATT 
CTGGGGAACC GTTGCTTATC TGAGGATGAG AAGAACACTC AACTTCAGGA ACTTATGCTA 
AAGAATCCTT CTGGATCTGG GCCTCCAGTC AGCATTGAGG ATTTGGGACT CAATTTCCAG 
TTCTGTCCAT CTTCCCGGGT TTATGGCTTC TCTGCAGTCG ATCTAAGGCC AAATGGAGAG 
GATGAGATGG TGACGATAGA CAACGCTGAG GAGTATGTTG ATCTTATGTT TGATTTCTGC 
ATGCAGACTG GTGTACAAAA GCAAATGGAA GCATTTAGAA GTGGCTTTAA TAAAGTGTTC 
CCTATGGAGA AACTGGGCTC CTTCAGCCCC GAGGAAGTCC AAATGATACT GTGTGGAAAC 
CAGTCTCCCT CTTGGTCCGC TGAGGACATC ATCAATTACA CAGAACCAAA GCTAGGATAT 
ACCCGAGAGA GTCCTGGGTT CCTTCGATTC GTGAGAGTCC TATGTGGCAT GTCGTCAGAT 
GAGAGGAAAG CGTTCCTGCA GTTTACAACG GGGTGCTCCA CATTGCCTCC AGGAGGACTG 
GCCAATCTGC ATCCGCGTCT CACAGTCGTG CGCAAGGTTG ATGCCACGGA TGCCAGCTAC 
CCATCTGTAA ATACATGTGT GCATTACCTG AAGCTGCCGG AGTATTCCTC CGAGGAAATC 
ATGAGAGACC GCCTGCTTGC TGCTACCATG GAGAAAGGAT TCCATCTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823551.1
CDS	305..7978
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVEGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDASLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAQHGLTEELLSRMAAAGGTVSGPSSACKTGRGTSGGPSTSGDSKISNQVSTIVSLLSTLCRGSPVVTHDLLRAELLDSMESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDEKKKKDSNREEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCHSDAGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGDDLFLDQLARLGVISKVSTLAGPTSDDENEEDSKPEKEDEPQEDAKELQQGRPYHWRDWSVIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPSTSSQPILSTPGPGKLTVGNWSLTCLKDGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESAWELHTNRQCIEGENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNNEDCDIKQDCGQLVERLNVFKTAFSENEDDESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETSLIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQSCVFRHQHDFDDNGIMYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKSAWFAIDLGLWVVPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTTLYTHMDDCSLNEPGSTATWPLDPAREEKQGWRHVRIKQTGKNASGQTHYLSLSGFELYGNVTGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGIDWKWRDQDGSAQGEGTVTGELHNGWIDVIWDAGGSNSYRMGAEGKFDLKLAPGYDPESAVSPKPVSSTVAGTPPSWSSLVKNNCPDKAPPSSSSSCVVVGSVAGSGSRKGSSSSVCSVASSSDVSLSCAKTERRAEEQVSDIHHDPILLLSSNQAASGSSTCPPGGETVGEGGDRKAGEAPAISMGMVSISSPDVSSVSELSNKEVAVPRPLGSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNAVSSATSPLMGAQSFPNLTTTGTTSTVTMSTSSVTSSNVATATTGLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEEQEYEEVMEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPAPGTPHSELLEEVECAPAPRLALTLKVTGLGSGREVELPLNNFRSTIFFYVQRLLQLSCNGAIKTDKLRRIWEPTYTIMYREMKDSDKQKECGRLGCWSVEHVEQSLGTDALPKNDLITYLQRNADPGFLRRWKLTGTNKSIRKNRNCSQLIAAYKDFCENGCKSLSMPAALATLQSADILSHSREQAQAKAGSSQNSCGVEDVLQLLRILFIVASDPYSARTPQEDGEDMLLFSVPPEEFTSKKITTKIVQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERSRTASAVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQKTDLGIWLCDDDFPDDESRQVDLGGGLKPPGYYVQRSCGLFIAPYPQDSEELDRVTRLCHFLGVFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLLYASRGEESEHCTESQSEASTEDGHDALSVGSFEEDCKSEFILDPPKPKPPAWFQGILTWEDFELINPHRARFLRDIRELAVKRRQILGNRCLSEDEKNTQLQELMLKNPSGSGPPVSIEDLGLNFQFCPSSRVYGFSAVDLRPNGEDEMVTIDNAEEYVDLMFDFCMQTGVQKQMEAFRSGFNKVFPMEKLGSFSPEEVQMILCGNQSPSWSAEDIINYTEPKLGYTRESPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRDRLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_012968097.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968097.3

ORF Insert Sequence:

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGAGGATGA GCCACAGGAA GATGCCAAGG AGTTGCAACA GGGCCGGCCC 
TACCATTGGC GGGACTGGTC TGTTATTCGC GGTAGGGACT GTTTGTACAT CTGGAGTGAT 
GCTGCAGCTC TGGAGCTCTC TAATGGTAGC AATGGCTGGT TCCGCTTTAT CCTAGATGGG 
AAACTGGCAA CCATGTATTC AAGTGGAAGC CCAGAGGGGG GTTCTGATAG CTCAGAAAGT 
AGAAGTGAAT TTTTAGAAAA GTTACAGCGG GCCCGCAGCC AGGTCAAACC ATCCACTTCT 
AGCCAGCCTA TCTTGTCCAC TCCTGGTCCA GGAAAGCTAA CAGTAGGCAA CTGGTCTCTC 
ACTTGTCTGA AAGATGGTGA GATTGCAATC CACAACTCGG ACGGGCAGCA GGCTACAATA 
CTGAAGGAGG ATCTACCAGG CTTTGTGTTT GAGTCAAACA GGGGTACCAA GCACTCCTTC 
ACGGCAGAAA CATCACTAGG ATCTGAATTT GTAACTGGCT GGACAGGAAA GAGAGGCAGA 
AAGCTGAAGT CTAAGTTGGA GAAAACTAAA CAGAAGGTAC GGACAATGGC AAGAGATTTG 
TATGATGATC ATTTTAAGGC AGTGGAAAGC ATGCCCCGAG GAGTGGTAGT GACGCTCCGA 
AATATTGCAA CACAGCTGGA ATCTGCTTGG GAACTTCATA CAAACAGACA GTGTATTGAA 
GGAGAGAACA CATGGAGAGA TCTGATGAAA ACTGCCCTGG AAAACCTGAT TGTGCTACTG 
AAGGATGAGA ATACTATTTC CCCTTATGAG ATGTGCAGCA GTGGACTGGT GCAGGCTTTG 
CTTACGGTGC TCAATAATAA TGAAGACTGT GATATAAAAC AAGACTGTGG GCAGCTGGTG 
GAGAGACTTA ATGTATTTAA AACTGCCTTC AGCGAAAATG AAGATGATGA AAGCCGCCCT 
GCCGTTGCCC TCGTCCGGAA GTTGATTGCT GTGCTTGAAT CAATAGAACG GCTGCCCCTT 
CACCTGTATG ACACCCCTGG ATCTAGCTAT AACTTGCAGA TCCTGACCCG TAGGCTTCGG 
TTTCGGCTGG AGCGTGCACC TGGAGAAACG TCTCTAATCG ACCGTACCGG TCGGATGCTG 
AAAATGGAGC CACTGGCAAC TGTAGAGTCC TTGGAGCAGT ATTTACTGAA AATGGTGGCT 
AAGCAGTGGT ACGACTTTGA CCGTTCGTCC TTTGTGTTTG TGCGCAAATT GCGCGAGGGC 
CAGAGTTGTG TGTTCCGCCA CCAGCATGAC TTTGATGATA ATGGAATCAT GTACTGGATT 
GGCACCAATG CAAAGACAGC TTATGAGTGG GTCAACCCTG CTGCTTACGG ACTAGTAGTT 
GTTACATCTT CTGAAGGCCG CAATTTGCCA TATGGCCGCC TTGAGGATAT CCTGAGCCGT 
GATAGCTCTG CCCTTAATTG CCACACTAAT GATGATAAGA GTGCCTGGTT TGCCATTGAC 
CTTGGCTTGT GGGTTGTGCC TTCTGCCTAT ACACTGCGTC ATGCTCGTGG ATATGGCCGT 
TCCGCTCTTC GCAATTGGGT CTTCCAGGTG TCTAAAGATG GTCAGAACTG GACCACCCTC 
TACACCCACA TGGATGACTG CAGCCTGAAT GAACCTGGCT CCACAGCCAC ATGGCCTCTG 
GACCCTGCGA GAGAAGAAAA GCAGGGTTGG CGTCATGTCC GGATAAAGCA GACGGGAAAG 
AATGCCAGTG GCCAGACCCA CTACCTCTCA CTTTCTGGCT TTGAGCTTTA TGGCAATGTG 
ACTGGGGTGT GTGAAGACCA ACTTGGTAAA GCTGCCAAAG AGGCAGAAGC CAACTTAAGG 
CGCCAGAGGC GGCTGGTACG CTCTCAGGTG CTAAAATATA TGGTGCCTGG AGCCCGTGTT 
ATTCGGGGCA TTGATTGGAA GTGGCGAGAC CAAGATGGCA GTGCTCAAGG AGAAGGCACT 
GTTACAGGAG AACTGCACAA TGGCTGGATT GATGTCATAT GGGATGCCGG TGGCTCTAAC 
TCTTACCGCA TGGGCGCGGA AGGAAAATTT GACCTGAAGC TGGCCCCGGG TTATGACCCA 
GAGTCTGCGG TGTCACCCAA ACCTGTTTCA TCCACTGTTG CAGGAACACC GCCCTCGTGG 
AGCAGCCTGG TGAAAAACAA CTGTCCTGAC AAAGCCCCTC CCTCTTCCTC ATCCTCTTGT 
GTGGTCGTTG GAAGCGTGGC GGGCTCAGGC AGCCGCAAAG GAAGCAGCAG CTCGGTCTGT 
AGCGTGGCAA GCAGCAGCGA CGTAAGCCTA AGCTGTGCAA AGACAGAGAG GAGGGCAGAG 
GAGCAAGTCT CCGACATTCA CCATGATCCC ATCCTGCTTC TGTCCTCTAA CCAAGCAGCC 
TCGGGTTCCT CCACCTGCCC CCCTGGAGGA GAGACTGTGG GTGAAGGTGG AGATAGAAAG 
GCAGGAGAAG CACCAGCAAT CTCAATGGGT ATGGTGAGCA TTAGCTCCCC TGATGTCAGT 
TCCGTTTCCG AACTCAGCAA CAAGGAAGTG GCAGTTCCAC GACCACTGGG ATCTTCTGCC 
AGCAACAGAT TGTCTGTCAG TTCACTGTTG GCAGCTGGGG CACCCATGAG TTCTAGCGCC 
AGTGTACCAA ACTTGTCCTC CAGGGAAACG TCCAGTCTGG AGTCCTTTGT AAGGAGAGTG 
GCAAATATCG CCAGGACAAA TGCAACCAAC AACATGAACC TGAGTCGTAG CAGCAGTGAT 
AACAATACCA ACACTCTGGG GAGGAATGCA GTCAGCTCAG CCACTTCACC ACTTATGGGA 
GCTCAGAGCT TTCCCAACCT GACGACAACA GGAACCACAT CCACCGTTAC AATGTCTACA 
TCCAGTGTCA CCAGCAGTAA TGTGGCAACA GCCACTACAG GGTTGTCTGT AGGTCAGTCC 
CTCAGTAACA CCCTGACTAC AAGCCTAACC TCGACATCCA GTGAGAGTGA CACTGGGCAG 
GAGGCAGAAT ACTCCCTGTA TGACTTTCTT GATAGCTGCC GTGCCAGCAC TTTGCTTGCT 
GAACTTGATG ATGACGAGGA TCTCCCTGAG CCCGATGAGG AAGATGATGA AAACGAAGAT 
GATAATCAGG AAGAGCAAGA GTATGAAGAA GTTATGGAGG AGGAAGAGTA TGAGACCAAA 
GGTGGCCGAC GCAGGACCTG GGATGATGAT TATGTGCTGA AGAGGCAGTT TTCTGCTTTG 
GTCCCGGCTT TTGACCCACG CCCAGGGAGA ACTAATGTTC AGCAGACCAC TGATCTTGAA 
ATTCCTGCTC CAGGCACGCC CCACTCTGAG CTACTGGAGG AGGTAGAATG TGCCCCAGCT 
CCACGATTAG CACTCACCCT AAAGGTGACA GGCCTTGGGA GCGGGAGAGA GGTGGAGCTC 
CCCCTGAACA ATTTCCGTTC CACCATATTT TTCTATGTAC AGAGACTGCT GCAACTCTCC 
TGCAATGGAG CCATCAAAAC TGACAAGCTG AGAAGGATTT GGGAGCCCAC CTACACGATT 
ATGTACAGAG AGATGAAGGA TTCAGATAAG CAAAAAGAAT GTGGCAGACT GGGCTGTTGG 
TCTGTAGAGC ATGTAGAACA ATCCCTAGGC ACAGATGCCT TGCCAAAGAA TGACCTGATC 
ACTTACCTGC AGCGAAATGC AGATCCAGGG TTCTTGCGAC GCTGGAAACT GACAGGAACC 
AACAAAAGCA TTAGGAAAAA CCGCAACTGT TCCCAGCTTA TTGCTGCTTA CAAGGACTTC 
TGTGAGAATG GCTGCAAGTC TCTCTCAATG CCAGCTGCTC TTGCCACACT CCAGAGTGCT 
GATATCCTTA GTCATTCCCG AGAGCAGGCA CAAGCCAAAG CGGGCAGCAG CCAAAACTCC 
TGCGGGGTAG AAGATGTTTT GCAGCTCCTG CGAATTCTCT TTATTGTAGC CAGTGACCCA 
TATTCTGCCA GGACCCCACA GGAAGATGGA GAAGATATGC TCCTTTTCAG TGTTCCTCCA 
GAGGAGTTTA CTAGCAAGAA AATCACCACA AAGATTGTTC AGCAAATCGA GGAACCTTTA 
GCTTTAGCAA GTGGAGCTTT GCCAGACTGG TGTGAACAGC TGACCAGCAA ATGTCCCTTC 
TTAATACCCT TTGAAACCCG GCAGCTTTAC TTCACATGCA CAGCATTTGG TGCCTCAAGG 
GCTATTGTGT GGCTGCAGAA CAGGCGGGAA GCCACAGTTG AGAGGAGCAG AACAGCAAGT 
GCAGTGCGCA GAGACGACCC AGGGGAGTTC AGAGTGGGCC GTCTAAAACA CGAACGTGTG 
AAGGTCCCAC GAGGGGAGTC TTTGATGGAG TGGGCAGAGA ATGTTATGCA AATCCACGCA 
GACCGAAAAT CTGTGCTTGA GGTGGAGTTT CTGGGAGAAG AAGGAACAGG CTTGGGCCCT 
ACCCTTGAGT TTTATGCCCT GGTGGCAGCA GAGTTCCAGA AAACAGATTT GGGGATCTGG 
TTATGTGATG ATGACTTCCC AGATGATGAG TCTCGGCAGG TGGATCTAGG TGGTGGTTTG 
AAGCCCCCTG GTTACTATGT GCAGCGTTCC TGTGGCCTCT TCATTGCACC CTACCCACAG 
GATAGTGAGG AACTAGACCG AGTGACCAGA CTTTGCCATT TCCTTGGTGT TTTCCTTGCA 
AAGTGCATCC AGGACAATAG ATTAGTGGAC TTGCCCATTT CAAAGCCGTT TTTTAAGCTT 
ATGTGTATGG GTGACATTAA GAGCAACATG AGCAAGCTTT TGTATGCCTC TCGGGGAGAG 
GAGAGTGAGC ACTGCACGGA GAGCCAGTCG GAGGCCTCTA CTGAGGATGG GCATGATGCC 
CTTTCAGTAG GAAGCTTTGA GGAAGACTGC AAATCTGAGT TCATCCTAGA CCCACCCAAA 
CCAAAGCCAC CAGCCTGGTT TCAGGGTATT TTAACTTGGG AGGACTTTGA GCTAATAAAC 
CCACATCGTG CTCGCTTCCT TAGGGACATT AGGGAGTTGG CTGTAAAGAG GAGACAGATT 
CTGGGGAACC GTTGCTTATC TGAGGATGAG AAGAACACTC AACTTCAGGA ACTTATGCTA 
AAGAATCCTT CTGGATCTGG GCCTCCAGTC AGCATTGAGG ATTTGGGACT CAATTTCCAG 
TTCTGTCCAT CTTCCCGGGT TTATGGCTTC TCTGCAGTCG ATCTAAGGCC AAATGGAGAG 
GATGAGATGG TGACGATAGA CAACGCTGAG GAGTATGTTG ATCTTATGTT TGATTTCTGC 
ATGCAGACTG GTGTACAAAA GCAAATGGAA GCATTTAGAA GTGGCTTTAA TAAAGTGTTC 
CCTATGGAGA AACTGGGCTC CTTCAGCCCC GAGGAAGTCC AAATGATACT GTGTGGAAAC 
CAGTCTCCCT CTTGGTCCGC TGAGGACATC ATCAATTACA CAGAACCAAA GCTAGGATAT 
ACCCGAGAGA GTCCTGGGTT CCTTCGATTC GTGAGAGTCC TATGTGGCAT GTCGTCAGAT 
GAGAGGAAAG CGTTCCTGCA GTTTACAACG GGGTGCTCCA CATTGCCTCC AGGAGGACTG 
GCCAATCTGC ATCCGCGTCT CACAGTCGTG CGCAAGGTTG ATGCCACGGA TGCCAGCTAC 
CCATCTGTAA ATACATGTGT GCATTACCTG AAGCTGCCGG AGTATTCCTC CGAGGAAATC 
ATGAGAGACC GCCTGCTTGC TGCTACCATG GAGAAAGGAT TCCATCTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd1 ( NM_001137613.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001131085.1 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), mRNA.

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CloneID	OXa22713
Clone ID Related Accession (Same CDS sequence)	NM_001137613.1
Accession Version	NM_001137613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168474.1. ##Evidence-Data-START## Transcript exon combination :: BC168474.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA 
GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG 
GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC 
TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC 
CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC 
TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA 
TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC 
TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG 
GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG 
CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG 
AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA 
AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG 
ACGCTTCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG 
TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT 
GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG 
CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG 
CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA 
AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG 
CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT 
AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT 
CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA 
ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG 
CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG 
TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA 
CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC 
CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT 
GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA 
TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG 
ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA 
TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG 
ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT 
GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC 
AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA 
GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA 
GAAGGCACTG TTACAGGAGA ACTGCACAAT GGAACACCGC CCTCGTGGAG CAGCCTGGTG 
AAAAACAACT GTCCTGACAA AGCCCCTCCC TCTTCCTCAT CCTCTTGTGT GGTCGTTGGA 
AGCGTGGCGG GCTCAGGCAG CCGCAAAGGA AGCAGCAGCT CGGTCTGTAG CGTGGCAAGC 
AGCAGCGACG TAAGCCTAAG CTGTGCAAAG ACAGAGAGGA GGGCAGAGGA GCAAGTCTCC 
GACATTCACC ATGATCCCAT CCTGCTTCTG TCCTCTAACC AAGCAGCCTC GGGTTCCTCC 
ACCTGCCCCC CTGGAGGAGA GACTGTGGGT GAAGGTGGAG ATAGAAAGGC AGGAGAAGCA 
CCAGCAATCT CAATGGGTAT GGTGAGCATT AGCTCCCCTG ATGTCAGTTC CGTTTCCGAA 
CTCAGCAACA AGGAAGTGGC AGTTCCACGA CCACTGGGAT CTTCTGCCAG CAACAGATTG 
TCTGTCAGTT CACTGTTGGC AGCTGGGGCA CCCATGAGTT CTAGCGCCAG TGTACCAAAC 
TTGTCCTCCA GGGAAACGTC CAGTCTGGAG TCCTTTGTAA GGAGAGTGGC AAATATCGCC 
AGGACAAATG CAACCAACAA CATGAACCTG AGTCGTAGCA GCAGTGATAA CAATACCAAC 
ACTCTGGGGA GGAATGCAGT CAGCTCAGCC ACTTCACCAC TTATGGGAGC TCAGAGCTTT 
CCCAACCTGA CGACAACAGG AACCACATCC ACTGTTACAA TGTCTACATC CAGTGTCACC 
AGCAGTAATG TGGCAACAGC CACTACAGGG TTGTCTGTAG GTCAGTCCCT CAGTAACACC 
CTGACTACAA GCCTAACCTC GACATCCAGT GAGAGTGACA CTGGGCAGGA GGCAGAATAC 
TCCCTGTATG ACTTTCTTGA TAGCTGCCGT GCCAGCACTT TGCTTGCTGA ACTTGATGAT 
GACGAGGATC TCCCTGAGCC CGATGAGGAA GATGATGAAA ACGAAGATGA TAATCAGGAA 
GAGCAAGAGT ATGAAGAAGT TATGGAGGAG GAAGAGTATG AGACCAAAGG TGGCCGACGC 
AGGACCTGGG ATGATGATTA TGTGCTGAAG AGGCAGTTTT CTGCTTTGGT CCCGGCTTTT 
GACCCACGCC CAGGGAGAAC TAATGTTCAG CAGACCACTG ATCTTGAAAT TCCTGCTCCA 
GGCACGCCCC ACTCTGAGCT ACTGGAGGAG GTAGAATGTG CCCCAGCTCC ACGATTAGCA 
CTCACCCTAA AGGTGACAGG CCTTGGGAGC GGGAGAGAGG TGGAGCTCCC CCTGAACAAT 
TTCCGTTCCA CCATATTTTT CTATGTACAG AGACTGCTGC AACTCTCCTG CAATGGAGCC 
ATCAAAACTG ACAAGCTGAG AAGGATTTGG GAGCCCACCT ACACGATTAT GTACAGAGAG 
ATGAAGGATT CAGATAAGCA AAAAGAATGT GGCAGACTGG GCTGTTGGTC TGTAGAGCAT 
GTGGAACAAT CCCTAGGCAC AGATGCCTTG CCAAAGAATG ACCTGATCAC TTACCTGCAG 
CGAAATGCAG ATCCAGGGTT CTTGCGACGC TGGAAACTGA CAGGAACCAA CAAAAGCATT 
AGGAAAAACC GCAACTGTTC CCAGCTTATT GCTGCTTACA AGGACTTCTG TGAGAATGGC 
TGCAAGTCTC TCTCAATGCC AGCTGCTCTT GCCACACTCC AGAGTGCTGA TATCCTTAGT 
CATTCCCGAG AGCAGGCACA AGCCAAAGCG GGCAGCAGCC AAAACTCCTG CGGGGTAGAA 
GATGTTTTGC AGCTCCTGCG AATTCTCTTT ATTGTAGCCA GTGACCCATA TTCTGCCAGG 
ACCCCACAGG AAGATGGAGA AGATATGCTC CTTTTCAGTG TTCCTCCAGA GGAGTTTACT 
AGCAAGAAAA TCACCACAAA GATTGTTCAG CAAATCGAGG AACCTTTAGC TTTAGCAAGT 
GGAGCTTTGC CAGACTGGTG TGAACAGCTG ACCAGCAAAT GTCCCTTCTT AATACCCTTT 
GAAACCCGGC AGCTTTACTT CACATGCACA GCATTTGGTG CCTCAAGGGC TATTGTGTGG 
CTGCAGAACA GGCGGGAAGC CACAGTTGAG AGGAGCAGAA CAGCAAGTGC AGTGCGCAGA 
GACGACCCAG GGGAGTTCAG AGTGGGCCGT CTAAAACACG AACGTGTGAA GGTCCCACGA 
GGGGAGTCTT TGATGGAGTG GGCAGAGAAT GTTATGCAAA TCCACGCAGA CCGAAAATCT 
GTGCTTGAGG TGGAGTTTCT GGGAGAAGAA GGAACAGGCT TGGGCCCTAC CCTTGAGTTT 
TATGCCCTGG TGGCAGCAGA GTTCCAGAAA ACAGATTTGG GGATCTGGTT ATGTGATGAT 
GACTTCCCAG ATGATGAGTC TCGGCAGGTG GATCTAGGTG GTGGTTTGAA GCCCCCTGGT 
TACTATGTGC AGCGTTCCTG TGGCCTCTTC ATTGCACCCT ACCCACAGGA TAGTGAGGAA 
CTAGACCGAG TGACCAGACT TTGCCATTTC CTTGGTGTTT TCCTTGCAAA GTGCATCCAG 
GACAATAGAT TAGTGGACTT GCCCATTTCA AAGCCGTTTT TTAAGCTTAT GTGTATGGGT 
GACATTAAGA GCAACATGAG CAAGCTTTTG TATGCCTCTC GGGGAGAGGA GAGTGAGCAC 
TGCACGGAGA GCCAGTCGGA GGCCTCTACT GAGGATGGGC ATGATGCCCT TTCAGTAGGA 
AGCTTTGAGG AAGACTGCAA ATCTGAGTTC ATCCTAGACC CACCCAAACC AAAGCCACCA 
GCCTGGTTTC AGGGTATTTT AACTTGGGAG GACTTTGAGC TAATAAACCC ACATCGTGCT 
CGCTTCCTTA GGGACATTAG GGAGTTGGCT GTAAAGAGGA GACAGATTCT GGGGAACCGT 
TGCTTATCTG AGGATGAGAA GAACACTCAA CTTCAGGAAC TTATGCTAAA GAATCCTTCT 
GGATCTGGGC CTCCAGTCAG CATTGAGGAT TTGGGACTCA ATTTCCAGTT CTGTCCATCT 
TCCCGGGTTT ATGGCTTCTC TGCAGTCGAT CTAAGGCCAA ATGGAGAGGA TGAGATGGTG 
ACGATAGACA ACGCTGAGGA GTATGTTGAT CTTATGTTTG ATTTCTGCAT GCAGACTGGT 
GTACAAAAGC AAATGGAAGC ATTTAGAAGT GGCTTTAATA AAGTGTTCCC TATGGAGAAA 
CTGGGCTCCT TCAGCCCCGA GGAAGTCCAA ATGATACTGT GTGGAAACCA GTCTCCCTCT 
TGGTCCGCTG AGGACATCAT CAATTACACA GAACCAAAGC TAGGATATAC CCGAGAGAGT 
CCTGGGTTCC TTCGATTCGT GAGAGTCCTA TGTGGCATGT CGTCAGATGA GAGGAAAGCG 
TTCCTGCAGT TTACAACGGG GTGCTCCACA TTGCCTCCAG GAGGACTGGC CAATCTGCAT 
CCGCGTCTCA CAGTCGTGCG CAAGGTTGAT GCCACGGATG CCAGCTACCC ATCTGTAAAT 
ACATGTGTGC ATTACCTGAA GCTGCCGGAG TATTCCTCCG AGGAAATCAT GAGAGACCGC 
CTGCTTGCTG CTACCATGGA GAAAGGATTC CATCTCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001131085.1
CDS	181..7782
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVEGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDASLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAQHGLTEELLSRMAAAGGTVSGPSSACKTGRGTSGGPSTSGDSKISNQVSTIVSLLSTLCRGSPVVTHDLLRAELLDSMESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDEKKKKDSNREEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCHSDAGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGDDLFLDQLARLGVISKVSTLAGPTSDDENEEDSKPEKVRIHLFPLYSAHKGTQAAASQLKEDEPQEDAKELQQGRPYHWRDWSVIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPSTSSQPILSTPGPGKLTVGNWSLTCLKDGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESAWELHTNRQCIEGENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNNEDCDIKQDCGQLVERLNVFKTAFSENEDDESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETSLIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQSCVFRHQHDFDDNGIMYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKSAWFAIDLGLWVVPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTTLYTHMDDCSLNEPGSTATWPLDPAREEKQGWRHVRIKQTGKNASGQTHYLSLSGFELYGNVTGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGIDWKWRDQDGSAQGEGTVTGELHNGTPPSWSSLVKNNCPDKAPPSSSSSCVVVGSVAGSGSRKGSSSSVCSVASSSDVSLSCAKTERRAEEQVSDIHHDPILLLSSNQAASGSSTCPPGGETVGEGGDRKAGEAPAISMGMVSISSPDVSSVSELSNKEVAVPRPLGSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNAVSSATSPLMGAQSFPNLTTTGTTSTVTMSTSSVTSSNVATATTGLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEEQEYEEVMEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPAPGTPHSELLEEVECAPAPRLALTLKVTGLGSGREVELPLNNFRSTIFFYVQRLLQLSCNGAIKTDKLRRIWEPTYTIMYREMKDSDKQKECGRLGCWSVEHVEQSLGTDALPKNDLITYLQRNADPGFLRRWKLTGTNKSIRKNRNCSQLIAAYKDFCENGCKSLSMPAALATLQSADILSHSREQAQAKAGSSQNSCGVEDVLQLLRILFIVASDPYSARTPQEDGEDMLLFSVPPEEFTSKKITTKIVQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERSRTASAVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQKTDLGIWLCDDDFPDDESRQVDLGGGLKPPGYYVQRSCGLFIAPYPQDSEELDRVTRLCHFLGVFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLLYASRGEESEHCTESQSEASTEDGHDALSVGSFEEDCKSEFILDPPKPKPPAWFQGILTWEDFELINPHRARFLRDIRELAVKRRQILGNRCLSEDEKNTQLQELMLKNPSGSGPPVSIEDLGLNFQFCPSSRVYGFSAVDLRPNGEDEMVTIDNAEEYVDLMFDFCMQTGVQKQMEAFRSGFNKVFPMEKLGSFSPEEVQMILCGNQSPSWSAEDIINYTEPKLGYTRESPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRDRLLAATMEKGFHLN

Target ORF information:

RefSeq Version	NM_001137613.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001137613.1

ORF Insert Sequence:

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA 
GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG 
GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC 
TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC 
CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC 
TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA 
TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC 
TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG 
GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG 
CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG 
AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA 
AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG 
ACGCTTCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG 
TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT 
GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG 
CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG 
CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA 
AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG 
CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT 
AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT 
CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA 
ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG 
CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG 
TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA 
CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC 
CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT 
GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA 
TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG 
ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA 
TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG 
ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT 
GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC 
AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA 
GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA 
GAAGGCACTG TTACAGGAGA ACTGCACAAT GGAACACCGC CCTCGTGGAG CAGCCTGGTG 
AAAAACAACT GTCCTGACAA AGCCCCTCCC TCTTCCTCAT CCTCTTGTGT GGTCGTTGGA 
AGCGTGGCGG GCTCAGGCAG CCGCAAAGGA AGCAGCAGCT CGGTCTGTAG CGTGGCAAGC 
AGCAGCGACG TAAGCCTAAG CTGTGCAAAG ACAGAGAGGA GGGCAGAGGA GCAAGTCTCC 
GACATTCACC ATGATCCCAT CCTGCTTCTG TCCTCTAACC AAGCAGCCTC GGGTTCCTCC 
ACCTGCCCCC CTGGAGGAGA GACTGTGGGT GAAGGTGGAG ATAGAAAGGC AGGAGAAGCA 
CCAGCAATCT CAATGGGTAT GGTGAGCATT AGCTCCCCTG ATGTCAGTTC CGTTTCCGAA 
CTCAGCAACA AGGAAGTGGC AGTTCCACGA CCACTGGGAT CTTCTGCCAG CAACAGATTG 
TCTGTCAGTT CACTGTTGGC AGCTGGGGCA CCCATGAGTT CTAGCGCCAG TGTACCAAAC 
TTGTCCTCCA GGGAAACGTC CAGTCTGGAG TCCTTTGTAA GGAGAGTGGC AAATATCGCC 
AGGACAAATG CAACCAACAA CATGAACCTG AGTCGTAGCA GCAGTGATAA CAATACCAAC 
ACTCTGGGGA GGAATGCAGT CAGCTCAGCC ACTTCACCAC TTATGGGAGC TCAGAGCTTT 
CCCAACCTGA CGACAACAGG AACCACATCC ACTGTTACAA TGTCTACATC CAGTGTCACC 
AGCAGTAATG TGGCAACAGC CACTACAGGG TTGTCTGTAG GTCAGTCCCT CAGTAACACC 
CTGACTACAA GCCTAACCTC GACATCCAGT GAGAGTGACA CTGGGCAGGA GGCAGAATAC 
TCCCTGTATG ACTTTCTTGA TAGCTGCCGT GCCAGCACTT TGCTTGCTGA ACTTGATGAT 
GACGAGGATC TCCCTGAGCC CGATGAGGAA GATGATGAAA ACGAAGATGA TAATCAGGAA 
GAGCAAGAGT ATGAAGAAGT TATGGAGGAG GAAGAGTATG AGACCAAAGG TGGCCGACGC 
AGGACCTGGG ATGATGATTA TGTGCTGAAG AGGCAGTTTT CTGCTTTGGT CCCGGCTTTT 
GACCCACGCC CAGGGAGAAC TAATGTTCAG CAGACCACTG ATCTTGAAAT TCCTGCTCCA 
GGCACGCCCC ACTCTGAGCT ACTGGAGGAG GTAGAATGTG CCCCAGCTCC ACGATTAGCA 
CTCACCCTAA AGGTGACAGG CCTTGGGAGC GGGAGAGAGG TGGAGCTCCC CCTGAACAAT 
TTCCGTTCCA CCATATTTTT CTATGTACAG AGACTGCTGC AACTCTCCTG CAATGGAGCC 
ATCAAAACTG ACAAGCTGAG AAGGATTTGG GAGCCCACCT ACACGATTAT GTACAGAGAG 
ATGAAGGATT CAGATAAGCA AAAAGAATGT GGCAGACTGG GCTGTTGGTC TGTAGAGCAT 
GTGGAACAAT CCCTAGGCAC AGATGCCTTG CCAAAGAATG ACCTGATCAC TTACCTGCAG 
CGAAATGCAG ATCCAGGGTT CTTGCGACGC TGGAAACTGA CAGGAACCAA CAAAAGCATT 
AGGAAAAACC GCAACTGTTC CCAGCTTATT GCTGCTTACA AGGACTTCTG TGAGAATGGC 
TGCAAGTCTC TCTCAATGCC AGCTGCTCTT GCCACACTCC AGAGTGCTGA TATCCTTAGT 
CATTCCCGAG AGCAGGCACA AGCCAAAGCG GGCAGCAGCC AAAACTCCTG CGGGGTAGAA 
GATGTTTTGC AGCTCCTGCG AATTCTCTTT ATTGTAGCCA GTGACCCATA TTCTGCCAGG 
ACCCCACAGG AAGATGGAGA AGATATGCTC CTTTTCAGTG TTCCTCCAGA GGAGTTTACT 
AGCAAGAAAA TCACCACAAA GATTGTTCAG CAAATCGAGG AACCTTTAGC TTTAGCAAGT 
GGAGCTTTGC CAGACTGGTG TGAACAGCTG ACCAGCAAAT GTCCCTTCTT AATACCCTTT 
GAAACCCGGC AGCTTTACTT CACATGCACA GCATTTGGTG CCTCAAGGGC TATTGTGTGG 
CTGCAGAACA GGCGGGAAGC CACAGTTGAG AGGAGCAGAA CAGCAAGTGC AGTGCGCAGA 
GACGACCCAG GGGAGTTCAG AGTGGGCCGT CTAAAACACG AACGTGTGAA GGTCCCACGA 
GGGGAGTCTT TGATGGAGTG GGCAGAGAAT GTTATGCAAA TCCACGCAGA CCGAAAATCT 
GTGCTTGAGG TGGAGTTTCT GGGAGAAGAA GGAACAGGCT TGGGCCCTAC CCTTGAGTTT 
TATGCCCTGG TGGCAGCAGA GTTCCAGAAA ACAGATTTGG GGATCTGGTT ATGTGATGAT 
GACTTCCCAG ATGATGAGTC TCGGCAGGTG GATCTAGGTG GTGGTTTGAA GCCCCCTGGT 
TACTATGTGC AGCGTTCCTG TGGCCTCTTC ATTGCACCCT ACCCACAGGA TAGTGAGGAA 
CTAGACCGAG TGACCAGACT TTGCCATTTC CTTGGTGTTT TCCTTGCAAA GTGCATCCAG 
GACAATAGAT TAGTGGACTT GCCCATTTCA AAGCCGTTTT TTAAGCTTAT GTGTATGGGT 
GACATTAAGA GCAACATGAG CAAGCTTTTG TATGCCTCTC GGGGAGAGGA GAGTGAGCAC 
TGCACGGAGA GCCAGTCGGA GGCCTCTACT GAGGATGGGC ATGATGCCCT TTCAGTAGGA 
AGCTTTGAGG AAGACTGCAA ATCTGAGTTC ATCCTAGACC CACCCAAACC AAAGCCACCA 
GCCTGGTTTC AGGGTATTTT AACTTGGGAG GACTTTGAGC TAATAAACCC ACATCGTGCT 
CGCTTCCTTA GGGACATTAG GGAGTTGGCT GTAAAGAGGA GACAGATTCT GGGGAACCGT 
TGCTTATCTG AGGATGAGAA GAACACTCAA CTTCAGGAAC TTATGCTAAA GAATCCTTCT 
GGATCTGGGC CTCCAGTCAG CATTGAGGAT TTGGGACTCA ATTTCCAGTT CTGTCCATCT 
TCCCGGGTTT ATGGCTTCTC TGCAGTCGAT CTAAGGCCAA ATGGAGAGGA TGAGATGGTG 
ACGATAGACA ACGCTGAGGA GTATGTTGAT CTTATGTTTG ATTTCTGCAT GCAGACTGGT 
GTACAAAAGC AAATGGAAGC ATTTAGAAGT GGCTTTAATA AAGTGTTCCC TATGGAGAAA 
CTGGGCTCCT TCAGCCCCGA GGAAGTCCAA ATGATACTGT GTGGAAACCA GTCTCCCTCT 
TGGTCCGCTG AGGACATCAT CAATTACACA GAACCAAAGC TAGGATATAC CCGAGAGAGT 
CCTGGGTTCC TTCGATTCGT GAGAGTCCTA TGTGGCATGT CGTCAGATGA GAGGAAAGCG 
TTCCTGCAGT TTACAACGGG GTGCTCCACA TTGCCTCCAG GAGGACTGGC CAATCTGCAT 
CCGCGTCTCA CAGTCGTGCG CAAGGTTGAT GCCACGGATG CCAGCTACCC ATCTGTAAAT 
ACATGTGTGC ATTACCTGAA GCTGCCGGAG TATTCCTCCG AGGAAATCAT GAGAGACCGC 
CTGCTTGCTG CTACCATGGA GAAAGGATTC CATCTCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd1 ( XM_012968096.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823550.1 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.

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CloneID	OXa32491
Clone ID Related Accession (Same CDS sequence)	XM_012968096.2 , XM_012968095.2
Accession Version	XM_012968096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA 
GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG 
GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC 
TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC 
CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC 
TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA 
TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC 
TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG 
GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG 
CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG 
AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA 
AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG 
ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG 
TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT 
GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG 
CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG 
CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA 
AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG 
CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT 
AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT 
CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA 
ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG 
CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG 
TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA 
CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC 
CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT 
GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA 
TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG 
ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA 
TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG 
ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT 
GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC 
AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA 
GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA 
GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT 
GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT 
TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG 
CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA 
TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC 
TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG 
AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC 
CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA 
GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT 
GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA 
TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT 
TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA 
AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC 
AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA 
CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACTGTTACA 
ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA 
GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC 
ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT 
TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA 
AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT 
GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT 
TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT 
GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT 
GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG TGGGAGAGAG 
GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG 
CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC 
TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG 
GGCTGTTGGT CTGTAGAGCA TGTGGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT 
GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG 
ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC 
AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC 
CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC 
CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC 
AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT 
GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG 
GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA 
TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT 
GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA 
ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC 
GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA 
ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC 
TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG 
GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT 
GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC 
TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT 
TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT 
TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT 
CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG 
CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC 
CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG 
CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG 
AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA 
CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC 
AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA 
AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT 
GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT 
AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG 
TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG 
CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG 
TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA 
GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT 
GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC 
GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823550.1
CDS	221..7963
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVEGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDASLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAQHGLTEELLSRMAAAGGTVSGPSSACKTGRGTSGGPSTSGDSKISNQVSTIVSLLSTLCRGSPVVTHDLLRAELLDSMESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDEKKKKDSNREEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCHSDAGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGDDLFLDQLARLGVISKVSTLAGPTSDDENEEDSKPEKVRIHLFPLYSAHKGTQAAASQLKEDEPQEDAKELQQGRPYHWRDWSVIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPSTSSQPILSTPGPGKLTVGNWSLTCLKDGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESAWELHTNRQCIEGENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNNEDCDIKQDCGQLVERLNVFKTAFSENEDDESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETSLIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQSCVFRHQHDFDDNGIMYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKSAWFAIDLGLWVVPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTTLYTHMDDCSLNEPGSTATWPLDPAREEKQGWRHVRIKQTGKNASGQTHYLSLSGFELYGNVTGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGIDWKWRDQDGSAQGEGTVTGELHNGWIDVIWDAGGSNSYRMGAEGKFDLKLAPGYDPESAVSPKPVSSTVAGTPPSWSSLVKNNCPDKAPPSSSSSCVVVGSVAGSGSRKGSSSSVCSVASSSDVSLSCAKTERRAEEQVSDIHHDPILLLSSNQAASGSSTCPPGGETVGEGGDRKAGEAPAISMGMVSISSPDVSSVSELSNKEVAVPRPLGSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNAVSSATSPLMGAQSFPNLTTTGTTSTVTMSTSSVTSSNVATATTGLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEEQEYEEVMEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPAPGTPHSELLEEVECAPAPRLALTLKVTGLGSGREVELPLNNFRSTIFFYVQRLLQLSCNGAIKTDKLRRIWEPTYTIMYREMKDSDKQKECGRLGCWSVEHVEQSLGTDALPKNDLITYLQRNADPGFLRRWKLTGTNKSIRKNRNCSQLIAAYKDFCENGCKSLSMPAALATLQSADILSHSREQAQAKAGSSQNSCGVEDVLQLLRILFIVASDPYSARTPQEDGEDMLLFSVPPEEFTSKKITTKIVQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERSRTASAVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQKTDLGIWLCDDDFPDDESRQVDLGGGLKPPGYYVQRSCGLFIAPYPQDSEELDRVTRLCHFLGVFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLLYASRGEESEHCTESQSEASTEDGHDALSVGSFEEDCKSEFILDPPKPKPPAWFQGILTWEDFELINPHRARFLRDIRELAVKRRQILGNRCLSEDEKNTQLQELMLKNPSGSGPPVSIEDLGLNFQFCPSSRVYGFSAVDLRPNGEDEMVTIDNAEEYVDLMFDFCMQTGVQKQMEAFRSGFNKVFPMEKLGSFSPEEVQMILCGNQSPSWSAEDIINYTEPKLGYTRESPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRDRLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_012968096.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968096.2

ORF Insert Sequence:

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA 
GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG 
GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC 
TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC 
CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC 
TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA 
TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC 
TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG 
GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG 
CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG 
AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA 
AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG 
ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG 
TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT 
GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG 
CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG 
CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA 
AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG 
CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT 
AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT 
CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA 
ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG 
CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG 
TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA 
CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC 
CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT 
GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA 
TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG 
ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA 
TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG 
ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT 
GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC 
AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA 
GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA 
GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT 
GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT 
TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG 
CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA 
TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC 
TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG 
AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC 
CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA 
GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT 
GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA 
TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT 
TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA 
AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC 
AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA 
CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACTGTTACA 
ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA 
GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC 
ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT 
TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA 
AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT 
GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT 
TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT 
GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT 
GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG TGGGAGAGAG 
GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG 
CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC 
TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG 
GGCTGTTGGT CTGTAGAGCA TGTGGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT 
GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG 
ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC 
AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC 
CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC 
CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC 
AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT 
GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG 
GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA 
TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT 
GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA 
ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC 
GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA 
ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC 
TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG 
GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT 
GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC 
TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT 
TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT 
TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT 
CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG 
CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC 
CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG 
CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG 
AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA 
CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC 
AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA 
AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT 
GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT 
AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG 
TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG 
CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG 
TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA 
GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT 
GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC 
GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd1 ( XM_012968095.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823549.1 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.

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CloneID	OXa32491
Clone ID Related Accession (Same CDS sequence)	XM_012968096.2 , XM_012968095.2
Accession Version	XM_012968095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA 
GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG 
GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC 
TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC 
CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC 
TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA 
TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC 
TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG 
GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG 
CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG 
AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA 
AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG 
ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG 
TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT 
GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG 
CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG 
CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA 
AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG 
CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT 
AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT 
CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA 
ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG 
CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG 
TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA 
CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC 
CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT 
GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA 
TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG 
ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA 
TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG 
ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT 
GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC 
AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA 
GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA 
GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT 
GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT 
TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG 
CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA 
TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC 
TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG 
AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC 
CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA 
GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT 
GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA 
TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT 
TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA 
AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC 
AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA 
CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACTGTTACA 
ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA 
GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC 
ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT 
TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA 
AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT 
GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT 
TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT 
GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT 
GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG TGGGAGAGAG 
GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG 
CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC 
TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG 
GGCTGTTGGT CTGTAGAGCA TGTGGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT 
GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG 
ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC 
AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC 
CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC 
CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC 
AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT 
GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG 
GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA 
TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT 
GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA 
ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC 
GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA 
ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC 
TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG 
GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT 
GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC 
TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT 
TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT 
TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT 
CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG 
CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC 
CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG 
CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG 
AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA 
CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC 
AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA 
AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT 
GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT 
AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG 
TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG 
CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG 
TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA 
GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT 
GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC 
GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823549.1
CDS	316..8058
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVEGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDASLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAQHGLTEELLSRMAAAGGTVSGPSSACKTGRGTSGGPSTSGDSKISNQVSTIVSLLSTLCRGSPVVTHDLLRAELLDSMESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDEKKKKDSNREEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCHSDAGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGDDLFLDQLARLGVISKVSTLAGPTSDDENEEDSKPEKVRIHLFPLYSAHKGTQAAASQLKEDEPQEDAKELQQGRPYHWRDWSVIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPSTSSQPILSTPGPGKLTVGNWSLTCLKDGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESAWELHTNRQCIEGENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNNEDCDIKQDCGQLVERLNVFKTAFSENEDDESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETSLIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQSCVFRHQHDFDDNGIMYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKSAWFAIDLGLWVVPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTTLYTHMDDCSLNEPGSTATWPLDPAREEKQGWRHVRIKQTGKNASGQTHYLSLSGFELYGNVTGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGIDWKWRDQDGSAQGEGTVTGELHNGWIDVIWDAGGSNSYRMGAEGKFDLKLAPGYDPESAVSPKPVSSTVAGTPPSWSSLVKNNCPDKAPPSSSSSCVVVGSVAGSGSRKGSSSSVCSVASSSDVSLSCAKTERRAEEQVSDIHHDPILLLSSNQAASGSSTCPPGGETVGEGGDRKAGEAPAISMGMVSISSPDVSSVSELSNKEVAVPRPLGSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNAVSSATSPLMGAQSFPNLTTTGTTSTVTMSTSSVTSSNVATATTGLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEEQEYEEVMEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPAPGTPHSELLEEVECAPAPRLALTLKVTGLGSGREVELPLNNFRSTIFFYVQRLLQLSCNGAIKTDKLRRIWEPTYTIMYREMKDSDKQKECGRLGCWSVEHVEQSLGTDALPKNDLITYLQRNADPGFLRRWKLTGTNKSIRKNRNCSQLIAAYKDFCENGCKSLSMPAALATLQSADILSHSREQAQAKAGSSQNSCGVEDVLQLLRILFIVASDPYSARTPQEDGEDMLLFSVPPEEFTSKKITTKIVQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERSRTASAVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQKTDLGIWLCDDDFPDDESRQVDLGGGLKPPGYYVQRSCGLFIAPYPQDSEELDRVTRLCHFLGVFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLLYASRGEESEHCTESQSEASTEDGHDALSVGSFEEDCKSEFILDPPKPKPPAWFQGILTWEDFELINPHRARFLRDIRELAVKRRQILGNRCLSEDEKNTQLQELMLKNPSGSGPPVSIEDLGLNFQFCPSSRVYGFSAVDLRPNGEDEMVTIDNAEEYVDLMFDFCMQTGVQKQMEAFRSGFNKVFPMEKLGSFSPEEVQMILCGNQSPSWSAEDIINYTEPKLGYTRESPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRDRLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_012968095.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968095.2

ORF Insert Sequence:

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA 
GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG 
GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC 
TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC 
CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC 
TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA 
TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC 
TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG 
GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG 
CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG 
AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA 
AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG 
ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG 
TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT 
GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG 
CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG 
CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA 
AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG 
CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT 
AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT 
CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA 
ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG 
CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG 
TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA 
CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC 
CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT 
GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA 
TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG 
ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA 
TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG 
ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT 
GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC 
AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA 
GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA 
GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT 
GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT 
TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG 
CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA 
TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC 
TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG 
AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC 
CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA 
GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT 
GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA 
TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT 
TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA 
AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC 
AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA 
CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACTGTTACA 
ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA 
GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC 
ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT 
TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA 
AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT 
GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT 
TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT 
GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT 
GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG TGGGAGAGAG 
GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG 
CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC 
TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG 
GGCTGTTGGT CTGTAGAGCA TGTGGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT 
GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG 
ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC 
AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC 
CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC 
CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC 
AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT 
GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG 
GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA 
TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT 
GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA 
ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC 
GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA 
ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC 
TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG 
GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT 
GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC 
TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT 
TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT 
TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT 
CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG 
CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC 
CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG 
CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG 
AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA 
CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC 
AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA 
AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT 
GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT 
AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG 
TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG 
CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG 
TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA 
GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT 
GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC 
GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd1 ( XM_012968097.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823551.1 Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.

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CloneID	OXa32492
Clone ID Related Accession (Same CDS sequence)	XM_012968097.2
Accession Version	XM_012968097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGAGGATGA GCCACAGGAA GATGCCAAGG AGTTGCAACA GGGCCGGCCC 
TACCATTGGC GGGACTGGTC TGTTATTCGC GGTAGGGACT GTTTGTACAT CTGGAGTGAT 
GCTGCAGCTC TGGAGCTCTC TAATGGTAGC AATGGCTGGT TCCGCTTTAT CCTAGATGGG 
AAACTGGCAA CCATGTATTC AAGTGGAAGC CCAGAGGGGG GTTCTGATAG CTCAGAAAGT 
AGAAGTGAAT TTTTAGAAAA GTTACAGCGG GCCCGCAGCC AGGTCAAACC ATCCACTTCT 
AGCCAGCCTA TCTTGTCCAC TCCTGGTCCA GGAAAGCTAA CAGTAGGCAA CTGGTCTCTC 
ACTTGTCTGA AAGATGGTGA GATTGCAATC CACAACTCGG ACGGGCAGCA GGCTACAATA 
CTGAAGGAGG ATCTACCAGG CTTTGTGTTT GAGTCAAACA GGGGTACCAA GCACTCCTTC 
ACGGCAGAAA CATCACTAGG ATCTGAATTT GTAACTGGCT GGACAGGAAA GAGAGGCAGA 
AAGCTGAAGT CTAAGTTGGA GAAAACTAAA CAGAAGGTAC GGACAATGGC AAGAGATTTG 
TATGATGATC ATTTTAAGGC AGTGGAAAGC ATGCCCCGAG GAGTGGTAGT GACGCTCCGA 
AATATTGCAA CACAGCTGGA ATCTGCTTGG GAACTTCATA CAAACAGACA GTGTATTGAA 
GGAGAGAACA CATGGAGAGA TCTGATGAAA ACTGCCCTGG AAAACCTGAT TGTGCTACTG 
AAGGATGAGA ATACTATTTC CCCTTATGAG ATGTGCAGCA GTGGACTGGT GCAGGCTTTG 
CTTACGGTGC TCAATAATAA TGAAGACTGT GATATAAAAC AAGACTGTGG GCAGCTGGTG 
GAGAGACTTA ATGTATTTAA AACTGCCTTC AGCGAAAATG AAGATGATGA AAGCCGCCCT 
GCCGTTGCCC TCGTCCGGAA GTTGATTGCT GTGCTTGAAT CAATAGAACG GCTGCCCCTT 
CACCTGTATG ACACCCCTGG ATCTAGCTAT AACTTGCAGA TCCTGACCCG TAGGCTTCGG 
TTTCGGCTGG AGCGTGCACC TGGAGAAACG TCTCTAATCG ACCGTACCGG TCGGATGCTG 
AAAATGGAGC CACTGGCAAC TGTAGAGTCC TTGGAGCAGT ATTTACTGAA AATGGTGGCT 
AAGCAGTGGT ACGACTTTGA CCGTTCGTCC TTTGTGTTTG TGCGCAAATT GCGCGAGGGC 
CAGAGTTGTG TGTTCCGCCA CCAGCATGAC TTTGATGATA ATGGAATCAT GTACTGGATT 
GGCACCAATG CAAAGACAGC TTATGAGTGG GTCAACCCTG CTGCTTACGG ACTAGTAGTT 
GTTACATCTT CTGAAGGCCG CAATTTGCCA TATGGCCGCC TTGAGGATAT CCTGAGCCGT 
GATAGCTCTG CCCTTAATTG CCACACTAAT GATGATAAGA GTGCCTGGTT TGCCATTGAC 
CTTGGCTTGT GGGTTGTGCC TTCTGCCTAT ACACTGCGTC ATGCTCGTGG ATATGGCCGT 
TCCGCTCTTC GCAATTGGGT CTTCCAGGTG TCTAAAGATG GTCAGAACTG GACCACCCTC 
TACACCCACA TGGATGACTG CAGCCTGAAT GAACCTGGCT CCACAGCCAC ATGGCCTCTG 
GACCCTGCGA GAGAAGAAAA GCAGGGTTGG CGTCATGTCC GGATAAAGCA GACGGGAAAG 
AATGCCAGTG GCCAGACCCA CTACCTCTCA CTTTCTGGCT TTGAGCTTTA TGGCAATGTG 
ACTGGGGTGT GTGAAGACCA ACTTGGTAAA GCTGCCAAAG AGGCAGAAGC CAACTTAAGG 
CGCCAGAGGC GGCTGGTACG CTCTCAGGTG CTAAAATATA TGGTGCCTGG AGCCCGTGTT 
ATTCGGGGCA TTGATTGGAA GTGGCGAGAC CAAGATGGCA GTGCTCAAGG AGAAGGCACT 
GTTACAGGAG AACTGCACAA TGGCTGGATT GATGTCATAT GGGATGCCGG TGGCTCTAAC 
TCTTACCGCA TGGGCGCGGA AGGAAAATTT GACCTGAAGC TGGCCCCGGG TTATGACCCA 
GAGTCTGCGG TGTCACCCAA ACCTGTTTCA TCCACTGTTG CAGGAACACC GCCCTCGTGG 
AGCAGCCTGG TGAAAAACAA CTGTCCTGAC AAAGCCCCTC CCTCTTCCTC ATCCTCTTGT 
GTGGTCGTTG GAAGCGTGGC GGGCTCAGGC AGCCGCAAAG GAAGCAGCAG CTCGGTCTGT 
AGCGTGGCAA GCAGCAGCGA CGTAAGCCTA AGCTGTGCAA AGACAGAGAG GAGGGCAGAG 
GAGCAAGTCT CCGACATTCA CCATGATCCC ATCCTGCTTC TGTCCTCTAA CCAAGCAGCC 
TCGGGTTCCT CCACCTGCCC CCCTGGAGGA GAGACTGTGG GTGAAGGTGG AGATAGAAAG 
GCAGGAGAAG CACCAGCAAT CTCAATGGGT ATGGTGAGCA TTAGCTCCCC TGATGTCAGT 
TCCGTTTCCG AACTCAGCAA CAAGGAAGTG GCAGTTCCAC GACCACTGGG ATCTTCTGCC 
AGCAACAGAT TGTCTGTCAG TTCACTGTTG GCAGCTGGGG CACCCATGAG TTCTAGCGCC 
AGTGTACCAA ACTTGTCCTC CAGGGAAACG TCCAGTCTGG AGTCCTTTGT AAGGAGAGTG 
GCAAATATCG CCAGGACAAA TGCAACCAAC AACATGAACC TGAGTCGTAG CAGCAGTGAT 
AACAATACCA ACACTCTGGG GAGGAATGCA GTCAGCTCAG CCACTTCACC ACTTATGGGA 
GCTCAGAGCT TTCCCAACCT GACGACAACA GGAACCACAT CCACTGTTAC AATGTCTACA 
TCCAGTGTCA CCAGCAGTAA TGTGGCAACA GCCACTACAG GGTTGTCTGT AGGTCAGTCC 
CTCAGTAACA CCCTGACTAC AAGCCTAACC TCGACATCCA GTGAGAGTGA CACTGGGCAG 
GAGGCAGAAT ACTCCCTGTA TGACTTTCTT GATAGCTGCC GTGCCAGCAC TTTGCTTGCT 
GAACTTGATG ATGACGAGGA TCTCCCTGAG CCCGATGAGG AAGATGATGA AAACGAAGAT 
GATAATCAGG AAGAGCAAGA GTATGAAGAA GTTATGGAGG AGGAAGAGTA TGAGACCAAA 
GGTGGCCGAC GCAGGACCTG GGATGATGAT TATGTGCTGA AGAGGCAGTT TTCTGCTTTG 
GTCCCGGCTT TTGACCCACG CCCAGGGAGA ACTAATGTTC AGCAGACCAC TGATCTTGAA 
ATTCCTGCTC CAGGCACGCC CCACTCTGAG CTACTGGAGG AGGTAGAATG TGCCCCAGCT 
CCACGATTAG CACTCACCCT AAAGGTGACA GGCCTTGGGA GTGGGAGAGA GGTGGAGCTC 
CCCCTGAACA ATTTCCGTTC CACCATATTT TTCTATGTAC AGAGACTGCT GCAACTCTCC 
TGCAATGGAG CCATCAAAAC TGACAAGCTG AGAAGGATTT GGGAGCCCAC CTACACGATT 
ATGTACAGAG AGATGAAGGA TTCAGATAAG CAAAAAGAAT GTGGCAGACT GGGCTGTTGG 
TCTGTAGAGC ATGTGGAACA ATCCCTAGGC ACAGATGCCT TGCCAAAGAA TGACCTGATC 
ACTTACCTGC AGCGAAATGC AGATCCAGGG TTCTTGCGAC GCTGGAAACT GACAGGAACC 
AACAAAAGCA TTAGGAAAAA CCGCAACTGT TCCCAGCTTA TTGCTGCTTA CAAGGACTTC 
TGTGAGAATG GCTGCAAGTC TCTCTCAATG CCAGCTGCTC TTGCCACACT CCAGAGTGCT 
GATATCCTTA GTCATTCCCG AGAGCAGGCA CAAGCCAAAG CGGGCAGCAG CCAAAACTCC 
TGCGGGGTAG AAGATGTTTT GCAGCTCCTG CGAATTCTCT TTATTGTAGC CAGTGACCCA 
TATTCTGCCA GGACCCCACA GGAAGATGGA GAAGATATGC TCCTTTTCAG TGTTCCTCCA 
GAGGAGTTTA CTAGCAAGAA AATCACCACA AAGATTGTTC AGCAAATCGA GGAACCTTTA 
GCTTTAGCAA GTGGAGCTTT GCCAGACTGG TGTGAACAGC TGACCAGCAA ATGTCCCTTC 
TTAATACCCT TTGAAACCCG GCAGCTTTAC TTCACATGCA CAGCATTTGG TGCCTCAAGG 
GCTATTGTGT GGCTGCAGAA CAGGCGGGAA GCCACAGTTG AGAGGAGCAG AACAGCAAGT 
GCAGTGCGCA GAGACGACCC AGGGGAGTTC AGAGTGGGCC GTCTAAAACA CGAACGTGTG 
AAGGTCCCAC GAGGGGAGTC TTTGATGGAG TGGGCAGAGA ATGTTATGCA AATCCACGCA 
GACCGAAAAT CTGTGCTTGA GGTGGAGTTT CTGGGAGAAG AAGGAACAGG CTTGGGCCCT 
ACCCTTGAGT TTTATGCCCT GGTGGCAGCA GAGTTCCAGA AAACAGATTT GGGGATCTGG 
TTATGTGATG ATGACTTCCC AGATGATGAG TCTCGGCAGG TGGATCTAGG TGGTGGTTTG 
AAGCCCCCTG GTTACTATGT GCAGCGTTCC TGTGGCCTCT TCATTGCACC CTACCCACAG 
GATAGTGAGG AACTAGACCG AGTGACCAGA CTTTGCCATT TCCTTGGTGT TTTCCTTGCA 
AAGTGCATCC AGGACAATAG ATTAGTGGAC TTGCCCATTT CAAAGCCGTT TTTTAAGCTT 
ATGTGTATGG GTGACATTAA GAGCAACATG AGCAAGCTTT TGTATGCCTC TCGGGGAGAG 
GAGAGTGAGC ACTGCACGGA GAGCCAGTCG GAGGCCTCTA CTGAGGATGG GCATGATGCC 
CTTTCAGTAG GAAGCTTTGA GGAAGACTGC AAATCTGAGT TCATCCTAGA CCCACCCAAA 
CCAAAGCCAC CAGCCTGGTT TCAGGGTATT TTAACTTGGG AGGACTTTGA GCTAATAAAC 
CCACATCGTG CTCGCTTCCT TAGGGACATT AGGGAGTTGG CTGTAAAGAG GAGACAGATT 
CTGGGGAACC GTTGCTTATC TGAGGATGAG AAGAACACTC AACTTCAGGA ACTTATGCTA 
AAGAATCCTT CTGGATCTGG GCCTCCAGTC AGCATTGAGG ATTTGGGACT CAATTTCCAG 
TTCTGTCCAT CTTCCCGGGT TTATGGCTTC TCTGCAGTCG ATCTAAGGCC AAATGGAGAG 
GATGAGATGG TGACGATAGA CAACGCTGAG GAGTATGTTG ATCTTATGTT TGATTTCTGC 
ATGCAGACTG GTGTACAAAA GCAAATGGAA GCATTTAGAA GTGGCTTTAA TAAAGTGTTC 
CCTATGGAGA AACTGGGCTC CTTCAGCCCC GAGGAAGTCC AAATGATACT GTGTGGAAAC 
CAGTCTCCCT CTTGGTCCGC TGAGGACATC ATCAATTACA CAGAACCAAA GCTAGGATAT 
ACCCGAGAGA GTCCTGGGTT CCTTCGATTC GTGAGAGTCC TATGTGGCAT GTCGTCAGAT 
GAGAGGAAAG CGTTCCTGCA GTTTACAACG GGGTGCTCCA CATTGCCTCC AGGAGGACTG 
GCCAATCTGC ATCCGCGTCT CACAGTCGTG CGCAAGGTTG ATGCCACGGA TGCCAGCTAC 
CCATCTGTAA ATACATGTGT GCATTACCTG AAGCTGCCGG AGTATTCCTC CGAGGAAATC 
ATGAGAGACC GCCTGCTTGC TGCTACCATG GAGAAAGGAT TCCATCTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823551.1
CDS	315..7988
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVEGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDASLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAQHGLTEELLSRMAAAGGTVSGPSSACKTGRGTSGGPSTSGDSKISNQVSTIVSLLSTLCRGSPVVTHDLLRAELLDSMESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDEKKKKDSNREEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCHSDAGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGDDLFLDQLARLGVISKVSTLAGPTSDDENEEDSKPEKEDEPQEDAKELQQGRPYHWRDWSVIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPSTSSQPILSTPGPGKLTVGNWSLTCLKDGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESAWELHTNRQCIEGENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNNEDCDIKQDCGQLVERLNVFKTAFSENEDDESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETSLIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQSCVFRHQHDFDDNGIMYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKSAWFAIDLGLWVVPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTTLYTHMDDCSLNEPGSTATWPLDPAREEKQGWRHVRIKQTGKNASGQTHYLSLSGFELYGNVTGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGIDWKWRDQDGSAQGEGTVTGELHNGWIDVIWDAGGSNSYRMGAEGKFDLKLAPGYDPESAVSPKPVSSTVAGTPPSWSSLVKNNCPDKAPPSSSSSCVVVGSVAGSGSRKGSSSSVCSVASSSDVSLSCAKTERRAEEQVSDIHHDPILLLSSNQAASGSSTCPPGGETVGEGGDRKAGEAPAISMGMVSISSPDVSSVSELSNKEVAVPRPLGSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNAVSSATSPLMGAQSFPNLTTTGTTSTVTMSTSSVTSSNVATATTGLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEEQEYEEVMEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPAPGTPHSELLEEVECAPAPRLALTLKVTGLGSGREVELPLNNFRSTIFFYVQRLLQLSCNGAIKTDKLRRIWEPTYTIMYREMKDSDKQKECGRLGCWSVEHVEQSLGTDALPKNDLITYLQRNADPGFLRRWKLTGTNKSIRKNRNCSQLIAAYKDFCENGCKSLSMPAALATLQSADILSHSREQAQAKAGSSQNSCGVEDVLQLLRILFIVASDPYSARTPQEDGEDMLLFSVPPEEFTSKKITTKIVQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERSRTASAVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQKTDLGIWLCDDDFPDDESRQVDLGGGLKPPGYYVQRSCGLFIAPYPQDSEELDRVTRLCHFLGVFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLLYASRGEESEHCTESQSEASTEDGHDALSVGSFEEDCKSEFILDPPKPKPPAWFQGILTWEDFELINPHRARFLRDIRELAVKRRQILGNRCLSEDEKNTQLQELMLKNPSGSGPPVSIEDLGLNFQFCPSSRVYGFSAVDLRPNGEDEMVTIDNAEEYVDLMFDFCMQTGVQKQMEAFRSGFNKVFPMEKLGSFSPEEVQMILCGNQSPSWSAEDIINYTEPKLGYTRESPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRDRLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_012968097.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968097.2

ORF Insert Sequence:

ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT 
GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA 
TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT 
TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA 
GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA 
GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG 
GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG 
GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG 
GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT 
CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT 
GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG 
GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC 
AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC 
AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA 
GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC 
ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG 
TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG 
CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT 
GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG 
AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC 
GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT 
CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA 
AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG 
TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT 
AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC 
CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC 
AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT 
AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA 
GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT 
AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT 
ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC 
AAGCCAGAGA AGGAGGATGA GCCACAGGAA GATGCCAAGG AGTTGCAACA GGGCCGGCCC 
TACCATTGGC GGGACTGGTC TGTTATTCGC GGTAGGGACT GTTTGTACAT CTGGAGTGAT 
GCTGCAGCTC TGGAGCTCTC TAATGGTAGC AATGGCTGGT TCCGCTTTAT CCTAGATGGG 
AAACTGGCAA CCATGTATTC AAGTGGAAGC CCAGAGGGGG GTTCTGATAG CTCAGAAAGT 
AGAAGTGAAT TTTTAGAAAA GTTACAGCGG GCCCGCAGCC AGGTCAAACC ATCCACTTCT 
AGCCAGCCTA TCTTGTCCAC TCCTGGTCCA GGAAAGCTAA CAGTAGGCAA CTGGTCTCTC 
ACTTGTCTGA AAGATGGTGA GATTGCAATC CACAACTCGG ACGGGCAGCA GGCTACAATA 
CTGAAGGAGG ATCTACCAGG CTTTGTGTTT GAGTCAAACA GGGGTACCAA GCACTCCTTC 
ACGGCAGAAA CATCACTAGG ATCTGAATTT GTAACTGGCT GGACAGGAAA GAGAGGCAGA 
AAGCTGAAGT CTAAGTTGGA GAAAACTAAA CAGAAGGTAC GGACAATGGC AAGAGATTTG 
TATGATGATC ATTTTAAGGC AGTGGAAAGC ATGCCCCGAG GAGTGGTAGT GACGCTCCGA 
AATATTGCAA CACAGCTGGA ATCTGCTTGG GAACTTCATA CAAACAGACA GTGTATTGAA 
GGAGAGAACA CATGGAGAGA TCTGATGAAA ACTGCCCTGG AAAACCTGAT TGTGCTACTG 
AAGGATGAGA ATACTATTTC CCCTTATGAG ATGTGCAGCA GTGGACTGGT GCAGGCTTTG 
CTTACGGTGC TCAATAATAA TGAAGACTGT GATATAAAAC AAGACTGTGG GCAGCTGGTG 
GAGAGACTTA ATGTATTTAA AACTGCCTTC AGCGAAAATG AAGATGATGA AAGCCGCCCT 
GCCGTTGCCC TCGTCCGGAA GTTGATTGCT GTGCTTGAAT CAATAGAACG GCTGCCCCTT 
CACCTGTATG ACACCCCTGG ATCTAGCTAT AACTTGCAGA TCCTGACCCG TAGGCTTCGG 
TTTCGGCTGG AGCGTGCACC TGGAGAAACG TCTCTAATCG ACCGTACCGG TCGGATGCTG 
AAAATGGAGC CACTGGCAAC TGTAGAGTCC TTGGAGCAGT ATTTACTGAA AATGGTGGCT 
AAGCAGTGGT ACGACTTTGA CCGTTCGTCC TTTGTGTTTG TGCGCAAATT GCGCGAGGGC 
CAGAGTTGTG TGTTCCGCCA CCAGCATGAC TTTGATGATA ATGGAATCAT GTACTGGATT 
GGCACCAATG CAAAGACAGC TTATGAGTGG GTCAACCCTG CTGCTTACGG ACTAGTAGTT 
GTTACATCTT CTGAAGGCCG CAATTTGCCA TATGGCCGCC TTGAGGATAT CCTGAGCCGT 
GATAGCTCTG CCCTTAATTG CCACACTAAT GATGATAAGA GTGCCTGGTT TGCCATTGAC 
CTTGGCTTGT GGGTTGTGCC TTCTGCCTAT ACACTGCGTC ATGCTCGTGG ATATGGCCGT 
TCCGCTCTTC GCAATTGGGT CTTCCAGGTG TCTAAAGATG GTCAGAACTG GACCACCCTC 
TACACCCACA TGGATGACTG CAGCCTGAAT GAACCTGGCT CCACAGCCAC ATGGCCTCTG 
GACCCTGCGA GAGAAGAAAA GCAGGGTTGG CGTCATGTCC GGATAAAGCA GACGGGAAAG 
AATGCCAGTG GCCAGACCCA CTACCTCTCA CTTTCTGGCT TTGAGCTTTA TGGCAATGTG 
ACTGGGGTGT GTGAAGACCA ACTTGGTAAA GCTGCCAAAG AGGCAGAAGC CAACTTAAGG 
CGCCAGAGGC GGCTGGTACG CTCTCAGGTG CTAAAATATA TGGTGCCTGG AGCCCGTGTT 
ATTCGGGGCA TTGATTGGAA GTGGCGAGAC CAAGATGGCA GTGCTCAAGG AGAAGGCACT 
GTTACAGGAG AACTGCACAA TGGCTGGATT GATGTCATAT GGGATGCCGG TGGCTCTAAC 
TCTTACCGCA TGGGCGCGGA AGGAAAATTT GACCTGAAGC TGGCCCCGGG TTATGACCCA 
GAGTCTGCGG TGTCACCCAA ACCTGTTTCA TCCACTGTTG CAGGAACACC GCCCTCGTGG 
AGCAGCCTGG TGAAAAACAA CTGTCCTGAC AAAGCCCCTC CCTCTTCCTC ATCCTCTTGT 
GTGGTCGTTG GAAGCGTGGC GGGCTCAGGC AGCCGCAAAG GAAGCAGCAG CTCGGTCTGT 
AGCGTGGCAA GCAGCAGCGA CGTAAGCCTA AGCTGTGCAA AGACAGAGAG GAGGGCAGAG 
GAGCAAGTCT CCGACATTCA CCATGATCCC ATCCTGCTTC TGTCCTCTAA CCAAGCAGCC 
TCGGGTTCCT CCACCTGCCC CCCTGGAGGA GAGACTGTGG GTGAAGGTGG AGATAGAAAG 
GCAGGAGAAG CACCAGCAAT CTCAATGGGT ATGGTGAGCA TTAGCTCCCC TGATGTCAGT 
TCCGTTTCCG AACTCAGCAA CAAGGAAGTG GCAGTTCCAC GACCACTGGG ATCTTCTGCC 
AGCAACAGAT TGTCTGTCAG TTCACTGTTG GCAGCTGGGG CACCCATGAG TTCTAGCGCC 
AGTGTACCAA ACTTGTCCTC CAGGGAAACG TCCAGTCTGG AGTCCTTTGT AAGGAGAGTG 
GCAAATATCG CCAGGACAAA TGCAACCAAC AACATGAACC TGAGTCGTAG CAGCAGTGAT 
AACAATACCA ACACTCTGGG GAGGAATGCA GTCAGCTCAG CCACTTCACC ACTTATGGGA 
GCTCAGAGCT TTCCCAACCT GACGACAACA GGAACCACAT CCACTGTTAC AATGTCTACA 
TCCAGTGTCA CCAGCAGTAA TGTGGCAACA GCCACTACAG GGTTGTCTGT AGGTCAGTCC 
CTCAGTAACA CCCTGACTAC AAGCCTAACC TCGACATCCA GTGAGAGTGA CACTGGGCAG 
GAGGCAGAAT ACTCCCTGTA TGACTTTCTT GATAGCTGCC GTGCCAGCAC TTTGCTTGCT 
GAACTTGATG ATGACGAGGA TCTCCCTGAG CCCGATGAGG AAGATGATGA AAACGAAGAT 
GATAATCAGG AAGAGCAAGA GTATGAAGAA GTTATGGAGG AGGAAGAGTA TGAGACCAAA 
GGTGGCCGAC GCAGGACCTG GGATGATGAT TATGTGCTGA AGAGGCAGTT TTCTGCTTTG 
GTCCCGGCTT TTGACCCACG CCCAGGGAGA ACTAATGTTC AGCAGACCAC TGATCTTGAA 
ATTCCTGCTC CAGGCACGCC CCACTCTGAG CTACTGGAGG AGGTAGAATG TGCCCCAGCT 
CCACGATTAG CACTCACCCT AAAGGTGACA GGCCTTGGGA GTGGGAGAGA GGTGGAGCTC 
CCCCTGAACA ATTTCCGTTC CACCATATTT TTCTATGTAC AGAGACTGCT GCAACTCTCC 
TGCAATGGAG CCATCAAAAC TGACAAGCTG AGAAGGATTT GGGAGCCCAC CTACACGATT 
ATGTACAGAG AGATGAAGGA TTCAGATAAG CAAAAAGAAT GTGGCAGACT GGGCTGTTGG 
TCTGTAGAGC ATGTGGAACA ATCCCTAGGC ACAGATGCCT TGCCAAAGAA TGACCTGATC 
ACTTACCTGC AGCGAAATGC AGATCCAGGG TTCTTGCGAC GCTGGAAACT GACAGGAACC 
AACAAAAGCA TTAGGAAAAA CCGCAACTGT TCCCAGCTTA TTGCTGCTTA CAAGGACTTC 
TGTGAGAATG GCTGCAAGTC TCTCTCAATG CCAGCTGCTC TTGCCACACT CCAGAGTGCT 
GATATCCTTA GTCATTCCCG AGAGCAGGCA CAAGCCAAAG CGGGCAGCAG CCAAAACTCC 
TGCGGGGTAG AAGATGTTTT GCAGCTCCTG CGAATTCTCT TTATTGTAGC CAGTGACCCA 
TATTCTGCCA GGACCCCACA GGAAGATGGA GAAGATATGC TCCTTTTCAG TGTTCCTCCA 
GAGGAGTTTA CTAGCAAGAA AATCACCACA AAGATTGTTC AGCAAATCGA GGAACCTTTA 
GCTTTAGCAA GTGGAGCTTT GCCAGACTGG TGTGAACAGC TGACCAGCAA ATGTCCCTTC 
TTAATACCCT TTGAAACCCG GCAGCTTTAC TTCACATGCA CAGCATTTGG TGCCTCAAGG 
GCTATTGTGT GGCTGCAGAA CAGGCGGGAA GCCACAGTTG AGAGGAGCAG AACAGCAAGT 
GCAGTGCGCA GAGACGACCC AGGGGAGTTC AGAGTGGGCC GTCTAAAACA CGAACGTGTG 
AAGGTCCCAC GAGGGGAGTC TTTGATGGAG TGGGCAGAGA ATGTTATGCA AATCCACGCA 
GACCGAAAAT CTGTGCTTGA GGTGGAGTTT CTGGGAGAAG AAGGAACAGG CTTGGGCCCT 
ACCCTTGAGT TTTATGCCCT GGTGGCAGCA GAGTTCCAGA AAACAGATTT GGGGATCTGG 
TTATGTGATG ATGACTTCCC AGATGATGAG TCTCGGCAGG TGGATCTAGG TGGTGGTTTG 
AAGCCCCCTG GTTACTATGT GCAGCGTTCC TGTGGCCTCT TCATTGCACC CTACCCACAG 
GATAGTGAGG AACTAGACCG AGTGACCAGA CTTTGCCATT TCCTTGGTGT TTTCCTTGCA 
AAGTGCATCC AGGACAATAG ATTAGTGGAC TTGCCCATTT CAAAGCCGTT TTTTAAGCTT 
ATGTGTATGG GTGACATTAA GAGCAACATG AGCAAGCTTT TGTATGCCTC TCGGGGAGAG 
GAGAGTGAGC ACTGCACGGA GAGCCAGTCG GAGGCCTCTA CTGAGGATGG GCATGATGCC 
CTTTCAGTAG GAAGCTTTGA GGAAGACTGC AAATCTGAGT TCATCCTAGA CCCACCCAAA 
CCAAAGCCAC CAGCCTGGTT TCAGGGTATT TTAACTTGGG AGGACTTTGA GCTAATAAAC 
CCACATCGTG CTCGCTTCCT TAGGGACATT AGGGAGTTGG CTGTAAAGAG GAGACAGATT 
CTGGGGAACC GTTGCTTATC TGAGGATGAG AAGAACACTC AACTTCAGGA ACTTATGCTA 
AAGAATCCTT CTGGATCTGG GCCTCCAGTC AGCATTGAGG ATTTGGGACT CAATTTCCAG 
TTCTGTCCAT CTTCCCGGGT TTATGGCTTC TCTGCAGTCG ATCTAAGGCC AAATGGAGAG 
GATGAGATGG TGACGATAGA CAACGCTGAG GAGTATGTTG ATCTTATGTT TGATTTCTGC 
ATGCAGACTG GTGTACAAAA GCAAATGGAA GCATTTAGAA GTGGCTTTAA TAAAGTGTTC 
CCTATGGAGA AACTGGGCTC CTTCAGCCCC GAGGAAGTCC AAATGATACT GTGTGGAAAC 
CAGTCTCCCT CTTGGTCCGC TGAGGACATC ATCAATTACA CAGAACCAAA GCTAGGATAT 
ACCCGAGAGA GTCCTGGGTT CCTTCGATTC GTGAGAGTCC TATGTGGCAT GTCGTCAGAT 
GAGAGGAAAG CGTTCCTGCA GTTTACAACG GGGTGCTCCA CATTGCCTCC AGGAGGACTG 
GCCAATCTGC ATCCGCGTCT CACAGTCGTG CGCAAGGTTG ATGCCACGGA TGCCAGCTAC 
CCATCTGTAA ATACATGTGT GCATTACCTG AAGCTGCCGG AGTATTCCTC CGAGGAAATC 
ATGAGAGACC GCCTGCTTGC TGCTACCATG GAGAAAGGAT TCCATCTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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hectd1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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