LOC100170623 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following LOC100170623 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100170623 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa52890 XM_031897307.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa42976 XM_018091853.1
Latest version!
Xenopus tropicalis poly [ADP-ribose] polymerase 4 (LOC100170623), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa52863 XM_031897278.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52864 XM_031897279.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52865 XM_031897280.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52866 XM_031897281.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52867 XM_031897282.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52868 XM_031897283.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52869 XM_031897284.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52870 XM_031897285.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52871 XM_031897286.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52872 XM_031897287.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52873 XM_031897288.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52874 XM_031897289.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52875 XM_031897290.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52876 XM_031897291.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52877 XM_031897292.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52878 XM_031897293.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52879 XM_031897294.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52879 XM_031897295.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52880 XM_031897296.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52881 XM_031897297.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52882 XM_031897299.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52882 XM_031897298.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52883 XM_031897300.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52884 XM_031897301.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52885 XM_031897302.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52886 XM_031897303.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52887 XM_031897304.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52888 XM_031897305.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52889 XM_031897306.1
Latest version!
Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa52890
    Clone ID Related Accession (Same CDS sequence) XM_031897307.1
    Accession Version XM_031897307.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGATCCT GAGAAAACTG TCTTCCCTAA AAAAGTTCCA
    TCCCTGCAAG ACAATGATGA GTTCATCAAC AGGGATTACA GTGAATATGA TGCAGACACC
    CTCCCCGACC CTCCAGATGC CGAAGTAGCA AAATACTGTT TTTTTCAAAA TGGTCCCATT
    GTGGCAGTTC TGGAGCTGTT GTGCATTCCT GAATCCTCTT CATTCCTATT TCAAATATCT
    AAAACAATTT GTAATTCCAA TGGATCGGAG CCTGACAGCT CCTATATGTT CAAACACACA
    GCGGAGGCGG CTTGTAACAA GTATGAGAAG TGCACTGAGG ATTTAGAAAA AGAAGGCTTT
    AAGAGAGCAA AGCGTATCCC ACCAAAGGTT GCCCTGCACA CATCTGAGGC TCTGCAGAAG
    GTCCTGCTTG AAGAAGCCAT AAATGCTACC AAGTTATCTC CAGATGTTGG ATATTTTGTG
    GAAAGTGTTT GGATTGAGGC TCTTGGCCAT TTGGATAACA TCCTCGCGCG TCCCGTAAAC
    AGCATCAGTC TGAATGATGT AAGCAAAGCA GAGGGTGTTC TGCTCCAGGT TAGGAAAGCT
    TTGGACACAG GAACAGAACC TGGAGACATA AGGAAAGCAA TGGCTGAGTT TTATCGGCTC
    ATTCCCCATA AGAAACCTCT GCTGGCCAAC ATTGATAAGA AACTTTTGTC AACAAAGCAA
    GATCTGTGCC AGCTTATTAG AGATATGGTG AGTGTATGTG AAACCAACTC ATCGAAGCCA
    AACCCAAGCA GCCTTGCCAA ATACCAGGCC CTGCGATGCC AAATAGAGCA TGTGGAGCCC
    AATACTGAGG AATTTCATCA GGTGTCACAG CAAATCGTGG AGAACAAACA CTGTGAGGAG
    TCGATTAACA TACTGAAAAT ATTCCGCGTT GGGAAATTAA GTGAAGCCAC TGGGTTTCAA
    AAGAATCTTG GGAATGTCAA GTCCCTGCTC CATGCATCTT CCCCACGTCA CTTTGTGGGG
    ATCCTTTCCC GTGGACTGCT CTTGCCAAAA AATGTGGAAG ATGATTTGGG TTTAAAAAGA
    ACTGATATTG GAAATCTTGG AAGTGGAATT TACTTCAGTG ATTCCATAAG TACAAGTGTT
    AAATATTCGG AACCCAGTGT AACCAATGGG GCGCGTCTCC TGTTGGTTTG TGAGGTGGCT
    CTGGGGAACT GCAAGGATCT CTACAAAAGA GACTTCTCCC TTACTGGAGC TCCAAGTGGC
    TTTAACAGCG TTCATGGTGT CCGACAAATG CCAGGGAGGA AATCTGATTT TGAGGATGAT
    GAATTTGTGG TATATAAAAA GGATCAAATA AAAATGAGAT ACGCCGTGCA GTTTTGCACT
    GATAAAGACC AAGTCAACAT GGAACGCGAC AAACTTTCTG TTCAGTTTGA GCCCATGGAG
    GAAGATGTTG GAGAAGAACC AGTGCAGGTG GAAGATCTCA TGTTGGAGAA TCTTCCGGAA
    CCACAGAGCA TAAATGGCGG CCTCCAGAGT GTTCATGGCA ACCAGATCCC TTTGGAAAAT
    ATTCATGTGA AAGGCAAAAT GATGGATTTA GTTGCAGAGG TTATATTATT CCAGACCTAC
    ATAAACAAAT CCTCTGTTCC CATCGAGGCT AAATATGTTT TTCCCCTGGA CGGCACAGCA
    GCCGTGTGTG GCTTTGAAGC ATTTATAAAT GGAAAACACG TTATCGGAGA GGTTAAAGAG
    AAGGAGCAAG CGCATAAGGA GTACAGGGCT GCTATCAGCG AGGGACATGG GGCTTACCTT
    ATGGACCAGG ACGCTGCTGA CGTCTTCACT GTAAGTGTTG GAAACCTGCC CCCAAACGCC
    ACTGTAATCA TTAAAATCAC GTACATAACG GAATTGATTC TGGAGTATGG ATCAGTGATA
    TTCAACATTC CTGGCACAGT GGCATCTTGG CAAAAGGATA AAGCTCTGAA AGAAAATACT
    CAGGATACAG TAGAAAAAGT CTGTGTTGAA GAAAGTGGTG TGCCACAAGG TTGTTTCTCT
    CTGGAGATGT CTGTTGAGAT GTCTTTTAAA ATCGAGAATG TGTTAAGTTT CACTCACAGT
    ATTTCAACGA AAAAAACTGA TTGCAAAGCT GTGATTGCAA CTAAAAAAGA CAGTTCATTG
    GATGACGAGG GGTTTCAGTT GAGAGTCTAC CTGGAATCAA GCTTCTTGGA GTCTTCTTGG
    AACCAACCGA GAATGTGGGT GGAAAAACAC CCCAAAGAAG ACAGTGAGGC ATGCATGCTT
    GTGTTCCTGC CTAGTTTTAA GACAAGCATA CAATCCTGGG ATTTAACCAT CTTACTTGAT
    TGCTCCAATT CTATGGAATC CACTTTCCAA AGCGCAAAAC GGATTGCTCT GCTTGCAGCC
    AGCTCGTTAA ACCCATGGCA CAACATAAAC ATAATCAGCT TTGGTACAGG TCATAAGGAA
    TTTAGCATCC GTCCAAAAGA GAGCCAGAAT TTAATACCGG AGCTGGAGCA GTTTATTAAA
    ATGGCCAAAC CAAACATGGG AAACACAGAA CTTTGGAAGC CTCTGCAGTC CTTATGTTTG
    CTGGCTCCTC CCAGTGACAT GCACAATGTT CTTCTTATAT CTGATGGACA CATCCAGAAT
    GAGAGCCTCG TCTTTCAGAT CCTCAAAAAG AATGCAGGGA AAGTGCGGCT GTTCACCTGT
    GGCGTTGGTG CAACAGCCAA TCGCCATATG TTGCGGTGTT TGGCTCAGTA CGGTGCTGGG
    TTCTTTGAAT TTTTTGAAGA TAAATCCAAG TCCAGCTGGA AAAAAAAGAT GGAGGCACAG
    CTGGAAAGAA TGGATTCCCC TGCATGCACT AGTGCATCTG TTAAATGGAT GCAGTTCAGT
    GATAATGACC CAGAGCCCGT TCAAGCCCCA GCACACATAC CGGCTTTGTT CAACCGCAGT
    TTCCTTCTTG TTTATGGCTT TGCTCCTCGT TGCACTCAGG CCAAGCTACA AGCTTTAATT
    GATGACAATG AACTGGACAC TATGGTATCA ACTACAGAAT TACAGAAGAC CAGAGGAACG
    ATGCTTCATA AACTGACGGC AAGGGCTGTT ATTAAGGATT ATGAAGATGG GATCCTGCAC
    GAGAAAGAAC ATGAACATGA GATGAAAAAG CAGCAAATGA AGTCCTTTAT TATTGAGCTG
    AGCAAGAAGT ACTCAATCGT CACTCAGTTT ACAAGCTTTG TCGCTGTAGA GAAACGGGAT
    GCACAAGAGA AGCCAAAGGA CTATGTACCA AATGTGCTTG AAATAATATC CACAGAAGAT
    GTTGATATAT TACCATACAT GATGTGGGAA ACACAGGAAG AGGATCAGGC CGCTGTGCTG
    CAGGAAGAAG ATATGCAGGA AGAAGAAGAA GAATATATGG ATCAAGAAGC ACCGGATTTG
    TCTGGGTCTG ATTTAGACGA TTATCGTCTG CCATTGTCTG GGTCTGATTC AGATGATTAT
    TGTTGTTATA TGGCTGACTT TGAATTGGCA AAAAGCAAGA AAAAAGCAAA ACGTGGACCT
    ATAGAAACAG AAGAGGCACA ACCTTTCAGA CGTATGAGAC GTGTAACTAC AATGTGTCAT
    ATGAGTCCTC CCCGATCAGA GGCACAACCT TCTGAACATG ATGGAGAGCG AGAATGGCGG
    ACGTTACATG CCGAGCAGGA AGTGGAGCGC ATAGGTGGAA GGCATGGTGG ACCGCATATC
    GATCGAGGGA ACAGGAGAGG CGCATGGATG CCAGAGCGAA CGGAGCCTGC GATGATGCGC
    CATATGGATC GTTGCGGAAC AGAGGCACAA CCTTCTGAAC ATGTGATGCA GGAAAGAGCT
    GCCAAGCCAA TGTGTCTTAT GAGTCTTCCC CGATCAGTGA TGCAGGAAAG AGCTGCCGAG
    GCAATGCGCC ATTTGGGTTT TCGCCGAACA GAAATCATGG ACATGCTGTC TCCAATGCCT
    CCATCCAACG TAAGTGTTCC TTGTAGTACA TCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753167.1
    CDS135..4433
    Translation

    Target ORF information:

    RefSeq Version XM_031897307.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897307.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGATCCT GAGAAAACTG TCTTCCCTAA AAAAGTTCCA
    TCCCTGCAAG ACAATGATGA GTTCATCAAC AGGGATTACA GTGAATATGA TGCAGACACC
    CTCCCCGACC CTCCAGATGC CGAAGTAGCA AAATACTGTT TTTTTCAAAA TGGTCCCATT
    GTGGCAGTTC TGGAGCTGTT GTGCATTCCT GAATCCTCTT CATTCCTATT TCAAATATCT
    AAAACAATTT GTAATTCCAA TGGATCGGAG CCTGACAGCT CCTATATGTT CAAACACACA
    GCGGAGGCGG CTTGTAACAA GTATGAGAAG TGCACTGAGG ATTTAGAAAA AGAAGGCTTT
    AAGAGAGCAA AGCGTATCCC ACCAAAGGTT GCCCTGCACA CATCTGAGGC TCTGCAGAAG
    GTCCTGCTTG AAGAAGCCAT AAATGCTACC AAGTTATCTC CAGATGTTGG ATATTTTGTG
    GAAAGTGTTT GGATTGAGGC TCTTGGCCAT TTGGATAACA TCCTCGCGCG TCCCGTAAAC
    AGCATCAGTC TGAATGATGT AAGCAAAGCA GAGGGTGTTC TGCTCCAGGT TAGGAAAGCT
    TTGGACACAG GAACAGAACC TGGAGACATA AGGAAAGCAA TGGCTGAGTT TTATCGGCTC
    ATTCCCCATA AGAAACCTCT GCTGGCCAAC ATTGATAAGA AACTTTTGTC AACAAAGCAA
    GATCTGTGCC AGCTTATTAG AGATATGGTG AGTGTATGTG AAACCAACTC ATCGAAGCCA
    AACCCAAGCA GCCTTGCCAA ATACCAGGCC CTGCGATGCC AAATAGAGCA TGTGGAGCCC
    AATACTGAGG AATTTCATCA GGTGTCACAG CAAATCGTGG AGAACAAACA CTGTGAGGAG
    TCGATTAACA TACTGAAAAT ATTCCGCGTT GGGAAATTAA GTGAAGCCAC TGGGTTTCAA
    AAGAATCTTG GGAATGTCAA GTCCCTGCTC CATGCATCTT CCCCACGTCA CTTTGTGGGG
    ATCCTTTCCC GTGGACTGCT CTTGCCAAAA AATGTGGAAG ATGATTTGGG TTTAAAAAGA
    ACTGATATTG GAAATCTTGG AAGTGGAATT TACTTCAGTG ATTCCATAAG TACAAGTGTT
    AAATATTCGG AACCCAGTGT AACCAATGGG GCGCGTCTCC TGTTGGTTTG TGAGGTGGCT
    CTGGGGAACT GCAAGGATCT CTACAAAAGA GACTTCTCCC TTACTGGAGC TCCAAGTGGC
    TTTAACAGCG TTCATGGTGT CCGACAAATG CCAGGGAGGA AATCTGATTT TGAGGATGAT
    GAATTTGTGG TATATAAAAA GGATCAAATA AAAATGAGAT ACGCCGTGCA GTTTTGCACT
    GATAAAGACC AAGTCAACAT GGAACGCGAC AAACTTTCTG TTCAGTTTGA GCCCATGGAG
    GAAGATGTTG GAGAAGAACC AGTGCAGGTG GAAGATCTCA TGTTGGAGAA TCTTCCGGAA
    CCACAGAGCA TAAATGGCGG CCTCCAGAGT GTTCATGGCA ACCAGATCCC TTTGGAAAAT
    ATTCATGTGA AAGGCAAAAT GATGGATTTA GTTGCAGAGG TTATATTATT CCAGACCTAC
    ATAAACAAAT CCTCTGTTCC CATCGAGGCT AAATATGTTT TTCCCCTGGA CGGCACAGCA
    GCCGTGTGTG GCTTTGAAGC ATTTATAAAT GGAAAACACG TTATCGGAGA GGTTAAAGAG
    AAGGAGCAAG CGCATAAGGA GTACAGGGCT GCTATCAGCG AGGGACATGG GGCTTACCTT
    ATGGACCAGG ACGCTGCTGA CGTCTTCACT GTAAGTGTTG GAAACCTGCC CCCAAACGCC
    ACTGTAATCA TTAAAATCAC GTACATAACG GAATTGATTC TGGAGTATGG ATCAGTGATA
    TTCAACATTC CTGGCACAGT GGCATCTTGG CAAAAGGATA AAGCTCTGAA AGAAAATACT
    CAGGATACAG TAGAAAAAGT CTGTGTTGAA GAAAGTGGTG TGCCACAAGG TTGTTTCTCT
    CTGGAGATGT CTGTTGAGAT GTCTTTTAAA ATCGAGAATG TGTTAAGTTT CACTCACAGT
    ATTTCAACGA AAAAAACTGA TTGCAAAGCT GTGATTGCAA CTAAAAAAGA CAGTTCATTG
    GATGACGAGG GGTTTCAGTT GAGAGTCTAC CTGGAATCAA GCTTCTTGGA GTCTTCTTGG
    AACCAACCGA GAATGTGGGT GGAAAAACAC CCCAAAGAAG ACAGTGAGGC ATGCATGCTT
    GTGTTCCTGC CTAGTTTTAA GACAAGCATA CAATCCTGGG ATTTAACCAT CTTACTTGAT
    TGCTCCAATT CTATGGAATC CACTTTCCAA AGCGCAAAAC GGATTGCTCT GCTTGCAGCC
    AGCTCGTTAA ACCCATGGCA CAACATAAAC ATAATCAGCT TTGGTACAGG TCATAAGGAA
    TTTAGCATCC GTCCAAAAGA GAGCCAGAAT TTAATACCGG AGCTGGAGCA GTTTATTAAA
    ATGGCCAAAC CAAACATGGG AAACACAGAA CTTTGGAAGC CTCTGCAGTC CTTATGTTTG
    CTGGCTCCTC CCAGTGACAT GCACAATGTT CTTCTTATAT CTGATGGACA CATCCAGAAT
    GAGAGCCTCG TCTTTCAGAT CCTCAAAAAG AATGCAGGGA AAGTGCGGCT GTTCACCTGT
    GGCGTTGGTG CAACAGCCAA TCGCCATATG TTGCGGTGTT TGGCTCAGTA CGGTGCTGGG
    TTCTTTGAAT TTTTTGAAGA TAAATCCAAG TCCAGCTGGA AAAAAAAGAT GGAGGCACAG
    CTGGAAAGAA TGGATTCCCC TGCATGCACT AGTGCATCTG TTAAATGGAT GCAGTTCAGT
    GATAATGACC CAGAGCCCGT TCAAGCCCCA GCACACATAC CGGCTTTGTT CAACCGCAGT
    TTCCTTCTTG TTTATGGCTT TGCTCCTCGT TGCACTCAGG CCAAGCTACA AGCTTTAATT
    GATGACAATG AACTGGACAC TATGGTATCA ACTACAGAAT TACAGAAGAC CAGAGGAACG
    ATGCTTCATA AACTGACGGC AAGGGCTGTT ATTAAGGATT ATGAAGATGG GATCCTGCAC
    GAGAAAGAAC ATGAACATGA GATGAAAAAG CAGCAAATGA AGTCCTTTAT TATTGAGCTG
    AGCAAGAAGT ACTCAATCGT CACTCAGTTT ACAAGCTTTG TCGCTGTAGA GAAACGGGAT
    GCACAAGAGA AGCCAAAGGA CTATGTACCA AATGTGCTTG AAATAATATC CACAGAAGAT
    GTTGATATAT TACCATACAT GATGTGGGAA ACACAGGAAG AGGATCAGGC CGCTGTGCTG
    CAGGAAGAAG ATATGCAGGA AGAAGAAGAA GAATATATGG ATCAAGAAGC ACCGGATTTG
    TCTGGGTCTG ATTTAGACGA TTATCGTCTG CCATTGTCTG GGTCTGATTC AGATGATTAT
    TGTTGTTATA TGGCTGACTT TGAATTGGCA AAAAGCAAGA AAAAAGCAAA ACGTGGACCT
    ATAGAAACAG AAGAGGCACA ACCTTTCAGA CGTATGAGAC GTGTAACTAC AATGTGTCAT
    ATGAGTCCTC CCCGATCAGA GGCACAACCT TCTGAACATG ATGGAGAGCG AGAATGGCGG
    ACGTTACATG CCGAGCAGGA AGTGGAGCGC ATAGGTGGAA GGCATGGTGG ACCGCATATC
    GATCGAGGGA ACAGGAGAGG CGCATGGATG CCAGAGCGAA CGGAGCCTGC GATGATGCGC
    CATATGGATC GTTGCGGAAC AGAGGCACAA CCTTCTGAAC ATGTGATGCA GGAAAGAGCT
    GCCAAGCCAA TGTGTCTTAT GAGTCTTCCC CGATCAGTGA TGCAGGAAAG AGCTGCCGAG
    GCAATGCGCC ATTTGGGTTT TCGCCGAACA GAAATCATGG ACATGCTGTC TCCAATGCCT
    CCATCCAACG TAAGTGTTCC TTGTAGTACA TCATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42976
    Clone ID Related Accession (Same CDS sequence) XM_018091853.1
    Accession Version XM_018091853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product poly [ADP-ribose] polymerase 4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 11% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AATTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGGGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGATCCT GAGAAAACTG TCTTCCCTAA AAAAGTTCCA
    TCCCTGCAAG ACAATGATGA GTTCATCAAC AGGGATTACA GTGAATATGA TGCAGACACC
    CTCCCCGACC CTCCAGATGC CGAAGTAGCA AAATACAGTT TTTTTCAAAA TGGTCCCATT
    GTGGCAGTTC TGGAGCTGTT GTGCATTCCT GAATCCTCTT CATTCCTATT TCAAATATCT
    AAAACAATTT GTAATTCCAA TGGATCGGAG CCTGACAGCT CCTATATGTT CAAACACACA
    GCGGAGGCGG CTTGTAACAA GTATGAGAAG TGCACTGAGG ATTTAGAAAA AGAAGGCTTT
    AAGAGAGCAA AGCGTATCCC ACCAAAGGTT GCCCTGCTCA CATCTGAGGC TCTGCAGAAG
    GTCCTGCTTG AAGAAGCCAT AAATGCTACC AAGTTATCTC CAGATGTTGG ATATTTTGTG
    GAAAGTGTTT GGATTGAGGC TCTTGGCCAT TTGGATAACA TCCTCGCGCG TCCCGTAAAC
    AGCATCAGTC TGAATGATGT AAGCAAAGCA GAGGGTGTTC TGCTCCAGGT TAGGAAAGCT
    TTGGACACAA GAACAGAACC TGGAGACATA AGGAAAGCAA TGGCTGAGTT TTATAGGCTC
    ATTCCCCATA AGAAACCTCT GCAGGCCAAA ATTGATAAGG AACTTTTGTC AACAAAGCAA
    GATCTGTGCC AGCTTATTAG AGATATGGTG AGTGTATGTG AAACCAACTC ATCAAAGCCA
    AACCCAAGCA GCCTTGCCAA ATACCAGGCC CTGCGATGCC AAATAGAGCA TGTGGAGCCC
    AATACTGAGG AATTTCATCA GGTGTCACAG CAAATCGTGG AGAACAAACA CTGTGAGGAG
    TCGATTAACA TACTGAAAAT ATTCCGCGTT GGGAAATTAA GTGAAGCCAC TGGGTTTCAA
    AAGAATCTTG GGAATGTCAA GTCCCTGCTC CATGCATCTT CCCCACGTCA CTTTGTGGGG
    ATCCTTTCCC GTGGACTGCT CTTGCCAAAA AATGTGGAAG ATGATTTGGG TTTAAAAAGA
    ACTGATATTG GAAATCTTGG AAGTGGAATT TACTTCAGTG ATTCCATAAG TACAAGTGTT
    AAATATTCGG AACCCAGTGT AACCAATGGG GCGCGTCTCC TGTTGGTTTG TGAGGTGGCT
    CTGGGGAACT GCAAGGATCT CTACAAAAGA GACTTCTCCC TTACTGGAGC TCCAAGTGGC
    TTTAACAGCG TTCATGGTGT CCGACAAATG CCAGGGAGGA AATCTGATTT TGAGGATGAT
    GAATTTGTGG TATATAAAAA GGATCAAATA AAAATGAGAT ACGCCGTGCA GTTTTGCACT
    GATAAAGACC AAGTCAACAT GGAACGCGAC AAACTTTCTG TTCAGTTTGA GCCCATGGAG
    GAAGATGTTG GAGAAGAACC AGTGCAGGTG GAAGATCTCA TGTTGGAGAA TCTTCCGGAA
    CCACAGAGCA TAAATGGCGG CCTCCAGAGT GTTCATGGCA ACCAGATCCC TTTGGAAAAT
    ATTCATGTGA AAGGCAAAAT GATGGATTTA GTTGCAGAGG TTATATTATT CCAGACCTAC
    ATAAACAAAT CCTCTGTTCC CATCGAGGCT AAATATGTTT TTCCCCTGGA CGGCACAGCA
    GCCGTGTGTG GCTTTGAAGC ATTTATAAAT GGAAAACACG TTATCGGAGA GGTTAAAGAG
    AAGGAGCAAG CGCATAAGGA GTACAGGGCT GCTATCAGCG AGGGACATGG GGCTTACCTT
    ATGGACCAGG ACGCTGCTGA CGTCTTCACT GTAAGTGTTG GAAACCTGCC CCCAAACGCC
    ACTGTAATCA TTAAAATCAC GTACATAACG GAATTGATTC TGGAGTATGG ATCAGTGATA
    TTCAACATTC CTGGCACAGT GGCATCTTGG CAAAAGGATA AAGCTCTGAA AGAAAATACT
    CAGGATACAG TAGAAAAAGT CTGTGTTGAA GAAAGTGGTG TGCCACAAGG TTGTTTCTCT
    CTGGAGATGT CTGTTGAGAT GTCTTTTAAA ATCGAGAATG TGTTAAGTTT CACTCACAGT
    ATTTCAACGA AAAAAACTGA TTGCAAAGCT GTGATTGCAA CTAAAAAAGA CAGTTCATTG
    GATGACGAGG GGTTTCAGTT GAGAGTCTAC CTGGAATCAA GCTTCTTGGA GTCTTCTTGG
    AACCAACCGA GAATGTGGGT GGAAAAACAC CCCAAAGAAG ACAGTGAGGC ATGCATGCTT
    GTGTTCCTGC CTAGTTTTAA GACAAGCATA CAATCCTGGG ATTTAACCAT CTTACTTGAT
    TGCTCCAATT CTATGGAATC CACTTTCCAA AGCGCAAAAC GGATTGCTCT GCTTGCAGCC
    AGCTCGTTAA ACCCATGGCA CAACATAAAC ATAATCAGCT TTGGTACAGG TCATAAGGAA
    TTTAGCATCC GTCCAAAAGA GAGCCAGAAT TTAATACCGG AGCTGGAGCA GTTTATTAAA
    ATGGCCAAAC CAAACATGGG AAACACAGAA CTTTGGAAGC CTCTGCAGTC CTTATGTTTG
    CTGGCTCCTC CCAGTGACAT GCACAACGTT CTTCTTATAT CTGATGGACA CATCCAGAAT
    GAGAGCCTCG TCTTTCAGAT CCTCAAAAAG AATGCAGGGA AAGTGCGGCT GTTCACCTGT
    GGCGTTGGTG CAACAGCCAA TCGCCATATG TTGCGGTGTT TGGCTCAGTA CGGTGCTGGG
    TTCTTTGAAT TTTTTGAAGA TAAATCCAAA ACCAGCTGGA AAAAAAAGAT GGAGGCACAG
    CTGGAAAGAA TGGATTCCCC TGCATGCACT AGTGCATCTG TTAAATGGAT GCAGTTCAGT
    GATAATGACC CAGAGCCCGT TCAAGCCCCA GCACACATAC CGGCTTTGTT CAACCGCAGT
    TTCCTTCTTG TTTATGGCTT TGCTCCTCGT TGCACTCAGG CCAAGCTACA AGCTTTAATT
    GATGACGATG AACTGGACAC TATGGTATCA ACTACGGAAT TACAGAAGAC CAGAGGAACG
    ATGCTTCATA AATTGACGGC AAGGGCTGTT ATTAAGGATT ATGAAGATGG GATCCTGCAC
    GAGAAAGAAC ATGAACATGA GATGAAAAAG CAGCAGATGA AGTCCTTTAT TATTGAGCTG
    AGCAAGAAGT ACTCAATCGT CACTCAGTTT ACAAGCTTTG TCGCTGTAGA GAAACGGGAT
    GCACAAGAGA AGCCAAAGGA CTATGTACCA AATGTGCTTG AAATAATATC CACAGAAGAT
    GTTGATATAT TACCATACAT GATGTGGGAA ACACAGGAAG AGGATCAGGC CGCTGTGCTG
    CAGGAAGAAA ATATGCAGGA AGAAGAAGAA GAAGATGTGC AGGAACAAGA AGAAGAATAT
    ATACAGGAAG AAGAATGTTA TATGGCTGAC TTTGAATTGG CAAAAAGCAA GAAAAAAGCA
    AAACGTGGAC CTATAGAAAC AGAAGAGGCA CAACCTTTCA GACGTATGAG ACGTGTAACT
    ACAATACGGA CAGGAACAGT CACTCTTTCG TCCAGCAGGT CCTGGATGAC GGAGAGGAAG
    GCATTCTGTT TGTTTGAATC TGGAGGGAGT TTTGACATGA AGAAGCGGAG AATTTTGGAC
    GGCCCTGGAA TCTCAGTTTG TTGGAGGACT GAGATTGATA GTCCCTGGAT GAAGATGATG
    ATGATGGCCT GGATGGCCTT CCACGAAAGA AGCGTCCTCA ACGAAAGAAG GGACGCCAAT
    TCCTGTCTAG GGGCGCCAGA ATTGATTCCT TCAATAAGGG GGTCCGACCA GGTTTGGATG
    GCCCGAGATA CCCATGCTGA TGCCAGAACC GGTCTCAGAC TTTCTCCGGC CGCTGTGTAC
    ACGGCTCTGA GGAAACCTTC CGTCTTTTTG TCCATGGAAT CCTTGAATGA GGAAGCGTCA
    GCGACCGGAA GGGCAGTGTT CTTGGAAAGG CGCGAGAAGA GTTTCTTGCG GGAATGGATA
    GGAGCGCTGG AACCTCCTAT TGGCTTGAAA GCGCTTCTCT GGTCTATTCA GGATGGAGAG
    CGAGAATGGC GGACGTTACA TGCCGAGCAG GAAGTGGAGC GCATAGGTGG AAGGCATGGT
    GGACCGCATA TCGATCGAGG GAACAGGAGA GGCGCATGGA TGCCAGAGCG AACGGAGCCT
    GCGATGATGC GCCATATGGA TCGTTGCGGA ACAGAGGCAC AACCTTCTGA ACATGTGATG
    CAGGAAAGAG CTGCCAAGCC AATGTGTCTT ATGAGTCTTC CCCGATCAGT GATGCAGGAA
    AGAGCTGCCG AGGCAATGCG CCATTTGGGT TTTCGCCGAA CAGAAATCAT GGACATGCTG
    TCTCCAATGC CTCCATCCAA CGTAAGTGTT CCTTGTAGTA CATCATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947342.1
    CDS116..4846
    Translation

    Target ORF information:

    RefSeq Version XM_018091853.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis poly [ADP-ribose] polymerase 4 (LOC100170623), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AATTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGGGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGATCCT GAGAAAACTG TCTTCCCTAA AAAAGTTCCA
    TCCCTGCAAG ACAATGATGA GTTCATCAAC AGGGATTACA GTGAATATGA TGCAGACACC
    CTCCCCGACC CTCCAGATGC CGAAGTAGCA AAATACAGTT TTTTTCAAAA TGGTCCCATT
    GTGGCAGTTC TGGAGCTGTT GTGCATTCCT GAATCCTCTT CATTCCTATT TCAAATATCT
    AAAACAATTT GTAATTCCAA TGGATCGGAG CCTGACAGCT CCTATATGTT CAAACACACA
    GCGGAGGCGG CTTGTAACAA GTATGAGAAG TGCACTGAGG ATTTAGAAAA AGAAGGCTTT
    AAGAGAGCAA AGCGTATCCC ACCAAAGGTT GCCCTGCTCA CATCTGAGGC TCTGCAGAAG
    GTCCTGCTTG AAGAAGCCAT AAATGCTACC AAGTTATCTC CAGATGTTGG ATATTTTGTG
    GAAAGTGTTT GGATTGAGGC TCTTGGCCAT TTGGATAACA TCCTCGCGCG TCCCGTAAAC
    AGCATCAGTC TGAATGATGT AAGCAAAGCA GAGGGTGTTC TGCTCCAGGT TAGGAAAGCT
    TTGGACACAA GAACAGAACC TGGAGACATA AGGAAAGCAA TGGCTGAGTT TTATAGGCTC
    ATTCCCCATA AGAAACCTCT GCAGGCCAAA ATTGATAAGG AACTTTTGTC AACAAAGCAA
    GATCTGTGCC AGCTTATTAG AGATATGGTG AGTGTATGTG AAACCAACTC ATCAAAGCCA
    AACCCAAGCA GCCTTGCCAA ATACCAGGCC CTGCGATGCC AAATAGAGCA TGTGGAGCCC
    AATACTGAGG AATTTCATCA GGTGTCACAG CAAATCGTGG AGAACAAACA CTGTGAGGAG
    TCGATTAACA TACTGAAAAT ATTCCGCGTT GGGAAATTAA GTGAAGCCAC TGGGTTTCAA
    AAGAATCTTG GGAATGTCAA GTCCCTGCTC CATGCATCTT CCCCACGTCA CTTTGTGGGG
    ATCCTTTCCC GTGGACTGCT CTTGCCAAAA AATGTGGAAG ATGATTTGGG TTTAAAAAGA
    ACTGATATTG GAAATCTTGG AAGTGGAATT TACTTCAGTG ATTCCATAAG TACAAGTGTT
    AAATATTCGG AACCCAGTGT AACCAATGGG GCGCGTCTCC TGTTGGTTTG TGAGGTGGCT
    CTGGGGAACT GCAAGGATCT CTACAAAAGA GACTTCTCCC TTACTGGAGC TCCAAGTGGC
    TTTAACAGCG TTCATGGTGT CCGACAAATG CCAGGGAGGA AATCTGATTT TGAGGATGAT
    GAATTTGTGG TATATAAAAA GGATCAAATA AAAATGAGAT ACGCCGTGCA GTTTTGCACT
    GATAAAGACC AAGTCAACAT GGAACGCGAC AAACTTTCTG TTCAGTTTGA GCCCATGGAG
    GAAGATGTTG GAGAAGAACC AGTGCAGGTG GAAGATCTCA TGTTGGAGAA TCTTCCGGAA
    CCACAGAGCA TAAATGGCGG CCTCCAGAGT GTTCATGGCA ACCAGATCCC TTTGGAAAAT
    ATTCATGTGA AAGGCAAAAT GATGGATTTA GTTGCAGAGG TTATATTATT CCAGACCTAC
    ATAAACAAAT CCTCTGTTCC CATCGAGGCT AAATATGTTT TTCCCCTGGA CGGCACAGCA
    GCCGTGTGTG GCTTTGAAGC ATTTATAAAT GGAAAACACG TTATCGGAGA GGTTAAAGAG
    AAGGAGCAAG CGCATAAGGA GTACAGGGCT GCTATCAGCG AGGGACATGG GGCTTACCTT
    ATGGACCAGG ACGCTGCTGA CGTCTTCACT GTAAGTGTTG GAAACCTGCC CCCAAACGCC
    ACTGTAATCA TTAAAATCAC GTACATAACG GAATTGATTC TGGAGTATGG ATCAGTGATA
    TTCAACATTC CTGGCACAGT GGCATCTTGG CAAAAGGATA AAGCTCTGAA AGAAAATACT
    CAGGATACAG TAGAAAAAGT CTGTGTTGAA GAAAGTGGTG TGCCACAAGG TTGTTTCTCT
    CTGGAGATGT CTGTTGAGAT GTCTTTTAAA ATCGAGAATG TGTTAAGTTT CACTCACAGT
    ATTTCAACGA AAAAAACTGA TTGCAAAGCT GTGATTGCAA CTAAAAAAGA CAGTTCATTG
    GATGACGAGG GGTTTCAGTT GAGAGTCTAC CTGGAATCAA GCTTCTTGGA GTCTTCTTGG
    AACCAACCGA GAATGTGGGT GGAAAAACAC CCCAAAGAAG ACAGTGAGGC ATGCATGCTT
    GTGTTCCTGC CTAGTTTTAA GACAAGCATA CAATCCTGGG ATTTAACCAT CTTACTTGAT
    TGCTCCAATT CTATGGAATC CACTTTCCAA AGCGCAAAAC GGATTGCTCT GCTTGCAGCC
    AGCTCGTTAA ACCCATGGCA CAACATAAAC ATAATCAGCT TTGGTACAGG TCATAAGGAA
    TTTAGCATCC GTCCAAAAGA GAGCCAGAAT TTAATACCGG AGCTGGAGCA GTTTATTAAA
    ATGGCCAAAC CAAACATGGG AAACACAGAA CTTTGGAAGC CTCTGCAGTC CTTATGTTTG
    CTGGCTCCTC CCAGTGACAT GCACAACGTT CTTCTTATAT CTGATGGACA CATCCAGAAT
    GAGAGCCTCG TCTTTCAGAT CCTCAAAAAG AATGCAGGGA AAGTGCGGCT GTTCACCTGT
    GGCGTTGGTG CAACAGCCAA TCGCCATATG TTGCGGTGTT TGGCTCAGTA CGGTGCTGGG
    TTCTTTGAAT TTTTTGAAGA TAAATCCAAA ACCAGCTGGA AAAAAAAGAT GGAGGCACAG
    CTGGAAAGAA TGGATTCCCC TGCATGCACT AGTGCATCTG TTAAATGGAT GCAGTTCAGT
    GATAATGACC CAGAGCCCGT TCAAGCCCCA GCACACATAC CGGCTTTGTT CAACCGCAGT
    TTCCTTCTTG TTTATGGCTT TGCTCCTCGT TGCACTCAGG CCAAGCTACA AGCTTTAATT
    GATGACGATG AACTGGACAC TATGGTATCA ACTACGGAAT TACAGAAGAC CAGAGGAACG
    ATGCTTCATA AATTGACGGC AAGGGCTGTT ATTAAGGATT ATGAAGATGG GATCCTGCAC
    GAGAAAGAAC ATGAACATGA GATGAAAAAG CAGCAGATGA AGTCCTTTAT TATTGAGCTG
    AGCAAGAAGT ACTCAATCGT CACTCAGTTT ACAAGCTTTG TCGCTGTAGA GAAACGGGAT
    GCACAAGAGA AGCCAAAGGA CTATGTACCA AATGTGCTTG AAATAATATC CACAGAAGAT
    GTTGATATAT TACCATACAT GATGTGGGAA ACACAGGAAG AGGATCAGGC CGCTGTGCTG
    CAGGAAGAAA ATATGCAGGA AGAAGAAGAA GAAGATGTGC AGGAACAAGA AGAAGAATAT
    ATACAGGAAG AAGAATGTTA TATGGCTGAC TTTGAATTGG CAAAAAGCAA GAAAAAAGCA
    AAACGTGGAC CTATAGAAAC AGAAGAGGCA CAACCTTTCA GACGTATGAG ACGTGTAACT
    ACAATACGGA CAGGAACAGT CACTCTTTCG TCCAGCAGGT CCTGGATGAC GGAGAGGAAG
    GCATTCTGTT TGTTTGAATC TGGAGGGAGT TTTGACATGA AGAAGCGGAG AATTTTGGAC
    GGCCCTGGAA TCTCAGTTTG TTGGAGGACT GAGATTGATA GTCCCTGGAT GAAGATGATG
    ATGATGGCCT GGATGGCCTT CCACGAAAGA AGCGTCCTCA ACGAAAGAAG GGACGCCAAT
    TCCTGTCTAG GGGCGCCAGA ATTGATTCCT TCAATAAGGG GGTCCGACCA GGTTTGGATG
    GCCCGAGATA CCCATGCTGA TGCCAGAACC GGTCTCAGAC TTTCTCCGGC CGCTGTGTAC
    ACGGCTCTGA GGAAACCTTC CGTCTTTTTG TCCATGGAAT CCTTGAATGA GGAAGCGTCA
    GCGACCGGAA GGGCAGTGTT CTTGGAAAGG CGCGAGAAGA GTTTCTTGCG GGAATGGATA
    GGAGCGCTGG AACCTCCTAT TGGCTTGAAA GCGCTTCTCT GGTCTATTCA GGATGGAGAG
    CGAGAATGGC GGACGTTACA TGCCGAGCAG GAAGTGGAGC GCATAGGTGG AAGGCATGGT
    GGACCGCATA TCGATCGAGG GAACAGGAGA GGCGCATGGA TGCCAGAGCG AACGGAGCCT
    GCGATGATGC GCCATATGGA TCGTTGCGGA ACAGAGGCAC AACCTTCTGA ACATGTGATG
    CAGGAAAGAG CTGCCAAGCC AATGTGTCTT ATGAGTCTTC CCCGATCAGT GATGCAGGAA
    AGAGCTGCCG AGGCAATGCG CCATTTGGGT TTTCGCCGAA CAGAAATCAT GGACATGCTG
    TCTCCAATGC CTCCATCCAA CGTAAGTGTT CCTTGTAGTA CATCATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52863
    Clone ID Related Accession (Same CDS sequence) XM_031897278.1
    Accession Version XM_031897278.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACC TCCGATACCT
    GTTGACAGGC TTCTGATGGC TACTGGCAAG CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC
    AAGGCTTTGT TCGCTCTAGC TCCTAGTGCT CCAACTTCAG ACTCCGAAGT TAAAGCAAGA
    ATTGCTGCTA AAATAAGAAG AGCCCCTCCA TCTTTCGTCC CCCTTTCAGC TCTACAGCAT
    GAGGAGGGAT ACTGGCTGCT GGATGAAAAG CTCGGATCTC TATTGAACAT TAATGTCAAT
    TACTTGATAG ATGTGTTTTT ATCCAAGAAC GGGATTCAGT CTCTTGGGAG GAAAGGAAAA
    GAAGAGGTGC TGGCACTTAT TGCGACCCTC TTGGTGCTGC AAATGATTCG CACGTATAAG
    TTGGTGGACA TAACATTTAA GAGTCTGATG AAACTGGACG AATCATTTTG CACAAGCCCC
    TTTTACCAGA CAATTGAGAA ATCCGTCAAA TGGGCACGAA AAAAAGATGA ACAATATCCT
    GGCATCTGTT CTCGACTTGG ATTGGGCAAG GATTGGGAAT CTGCCACCCG GAAGTTATTA
    TCCATTGATC CAGTGGATCT TTCCTCTGAT CTCTATCCTG CTGTTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753138.1
    CDS135..5645
    Translation

    Target ORF information:

    RefSeq Version XM_031897278.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897278.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACC TCCGATACCT
    GTTGACAGGC TTCTGATGGC TACTGGCAAG CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC
    AAGGCTTTGT TCGCTCTAGC TCCTAGTGCT CCAACTTCAG ACTCCGAAGT TAAAGCAAGA
    ATTGCTGCTA AAATAAGAAG AGCCCCTCCA TCTTTCGTCC CCCTTTCAGC TCTACAGCAT
    GAGGAGGGAT ACTGGCTGCT GGATGAAAAG CTCGGATCTC TATTGAACAT TAATGTCAAT
    TACTTGATAG ATGTGTTTTT ATCCAAGAAC GGGATTCAGT CTCTTGGGAG GAAAGGAAAA
    GAAGAGGTGC TGGCACTTAT TGCGACCCTC TTGGTGCTGC AAATGATTCG CACGTATAAG
    TTGGTGGACA TAACATTTAA GAGTCTGATG AAACTGGACG AATCATTTTG CACAAGCCCC
    TTTTACCAGA CAATTGAGAA ATCCGTCAAA TGGGCACGAA AAAAAGATGA ACAATATCCT
    GGCATCTGTT CTCGACTTGG ATTGGGCAAG GATTGGGAAT CTGCCACCCG GAAGTTATTA
    TCCATTGATC CAGTGGATCT TTCCTCTGAT CTCTATCCTG CTGTTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52864
    Clone ID Related Accession (Same CDS sequence) XM_031897279.1
    Accession Version XM_031897279.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGATCCT GAGAAAACTG TCTTCCCTAA AAAAGTTCCA
    TCCCTGCAAG ACAATGATGA GTTCATCAAC AGGGATTACA GTGAATATGA TGCAGACACC
    CTCCCCGACC CTCCAGATGC CGAAGTAGCA AAATACTGTT TTTTTCAAAA TGGTCCCATT
    GTGGCAGTTC TGGAGCTGTT GTGCATTCCT GAATCCTCTT CATTCCTATT TCAAATATCT
    AAAACAATTT GTAATTCCAA TGGATCGGAG CCTGACAGCT CCTATATGTT CAAACACACA
    GCGGAGGCGG CTTGTAACAA GTATGAGAAG TGCACTGAGG ATTTAGAAAA AGAAGGCTTT
    AAGAGAGCAA AGCGTATCCC ACCAAAGGTT GCCCTGCACA CATCTGAGGC TCTGCAGAAG
    GTCCTGCTTG AAGAAGCCAT AAATGCTACC AAGTTATCTC CAGATGTTGG ATATTTTGTG
    GAAAGTGTTT GGATTGAGGC TCTTGGCCAT TTGGATAACA TCCTCGCGCG TCCCGTAAAC
    AGCATCAGTC TGAATGATGT AAGCAAAGCA GAGGGTGTTC TGCTCCAGGT TAGGAAAGCT
    TTGGACACAG GAACAGAACC TGGAGACATA AGGAAAGCAA TGGCTGAGTT TTATCGGCTC
    ATTCCCCATA AGAAACCTCT GCTGGCCAAC ATTGATAAGA AACTTTTGTC AACAAAGCAA
    GATCTGTGCC AGCTTATTAG AGATATGGTG AGTGTATGTG AAACCAACTC ATCGAAGCCA
    AACCCAAGCA GCCTTGCCAA ATACCAGGCC CTGCGATGCC AAATAGAGCA TGTGGAGCCC
    AATACTGAGG AATTTCATCA GGTGTCACAG CAAATCGTGG AGAACAAACA CTGTGAGGAG
    TCGATTAACA TACTGAAAAT ATTCCGCGTT GGGAAATTAA GTGAAGCCAC TGGGTTTCAA
    AAGAATCTTG GGAATGTCAA GTCCCTGCTC CATGCATCTT CCCCACGTCA CTTTGTGGGG
    ATCCTTTCCC GTGGACTGCT CTTGCCAAAA AATGTGGAAG ATGATTTGGG TTTAAAAAGA
    ACTGATATTG GAAATCTTGG AAGTGGAATT TACTTCAGTG ATTCCATAAG TACAAGTGTT
    AAATATTCGG AACCCAGTGT AACCAATGGG GCGCGTCTCC TGTTGGTTTG TGAGGTGGCT
    CTGGGGAACT GCAAGGATCT CTACAAAAGA GACTTCTCCC TTACTGGAGC TCCAAGTGGC
    TTTAACAGCG TTCATGGTGT CCGACAAATG CCAGGGAGGA AATCTGATTT TGAGGATGAT
    GAATTTGTGG TATATAAAAA GGATCAAATA AAAATGAGAT ACGCCGTGCA GTTTTGCACT
    GATAAAGACC AAGTCAACAT GGAACGCGAC AAACTTTCTG TTCAGTTTGA GCCCATGGAG
    GAAGATGTTG GAGAAGAACC AGTGCAGGTG GAAGATCTCA TGTTGGAGAA TCTTCCGGAA
    CCACAGAGCA TAAATGGCGG CCTCCAGAGT GTTCATGGCA ACCAGATCCC TTTGGAAAAT
    ATTCATGTGA AAGGCAAAAT GATGGATTTA GTTGCAGAGG TTATATTATT CCAGACCTAC
    ATAAACAAAT CCTCTGTTCC CATCGAGGCT AAATATGTTT TTCCCCTGGA CGGCACAGCA
    GCCGTGTGTG GCTTTGAAGC ATTTATAAAT GGAAAACACG TTATCGGAGA GGTTAAAGAG
    AAGGAGCAAG CGCATAAGGA GTACAGGGCT GCTATCAGCG AGGGACATGG GGCTTACCTT
    ATGGACCAGG ACGCTGCTGA CGTCTTCACT GTAAGTGTTG GAAACCTGCC CCCAAACGCC
    ACTGTAATCA TTAAAATCAC GTACATAACG GAATTGATTC TGGAGTATGG ATCAGTGATA
    TTCAACATTC CTGGCACAGT GGCATCTTGG CAAAAGGATA AAGCTCTGAA AGAAAATACT
    CAGGATACAG TAGAAAAAGT CTGTGTTGAA GAAAGTGGTG TGCCACAAGG TTGTTTCTCT
    CTGGAGATGT CTGTTGAGAT GTCTTTTAAA ATCGAGAATG TGTTAAGTTT CACTCACAGT
    ATTTCAACGA AAAAAACTGA TTGCAAAGCT GTGATTGCAA CTAAAAAAGA CAGTTCATTG
    GATGACGAGG GGTTTCAGTT GAGAGTCTAC CTGGAATCAA GCTTCTTGGA GTCTTCTTGG
    AACCAACCGA GAATGTGGGT GGAAAAACAC CCCAAAGAAG ACAGTGAGGC ATGCATGCTT
    GTGTTCCTGC CTAGTTTTAA GACAAGCATA CAATCCTGGG ATTTAACCAT CTTACTTGAT
    TGCTCCAATT CTATGGAATC CACTTTCCAA AGCGCAAAAC GGATTGCTCT GCTTGCAGCC
    AGCTCGTTAA ACCCATGGCA CAACATAAAC ATAATCAGCT TTGGTACAGG TCATAAGGAA
    TTTAGCATCC GTCCAAAAGA GAGCCAGAAT TTAATACCGG AGCTGGAGCA GTTTATTAAA
    ATGGCCAAAC CAAACATGGG AAACACAGAA CTTTGGAAGC CTCTGCAGTC CTTATGTTTG
    CTGGCTCCTC CCAGTGACAT GCACAATGTT CTTCTTATAT CTGATGGACA CATCCAGAAT
    GAGAGCCTCG TCTTTCAGAT CCTCAAAAAG AATGCAGGGA AAGTGCGGCT GTTCACCTGT
    GGCGTTGGTG CAACAGCCAA TCGCCATATG TTGCGGTGTT TGGCTCAGTA CGGTGCTGGG
    TTCTTTGAAT TTTTTGAAGA TAAATCCAAG TCCAGCTGGA AAAAAAAGAT GGAGGCACAG
    CTGGAAAGAA TGGATTCCCC TGCATGCACT AGTGCATCTG TTAAATGGAT GCAGTTCAGT
    GATAATGACC CAGAGCCCGT TCAAGCCCCA GCACACATAC CGGCTTTGTT CAACCGCAGT
    TTCCTTCTTG TTTATGGCTT TGCTCCTCGT TGCACTCAGG CCAAGCTACA AGCTTTAATT
    GATGACAATG AACTGGACAC TATGGTATCA ACTACAGAAT TACAGAAGAC CAGAGGAACG
    ATGCTTCATA AACTGACGGC AAGGGCTGTT ATTAAGGATT ATGAAGATGG GATCCTGCAC
    GAGAAAGAAC ATGAACATGA GATGAAAAAG CAGCAAATGA AGTCCTTTAT TATTGAGCTG
    AGCAAGAAGT ACTCAATCGT CACTCAGTTT ACAAGCTTTG TCGCTGTAGA GAAACGGGAT
    GCACAAGAGA AGCCAAAGGA CTATGTACCA AATGTGCTTG AAATAATATC CACAGAAGAT
    GTTGATATAT TACCATACAT GATGTGGGAA ACACAGGAAG AGGATCAGGC CGCTGTGCTG
    CAGGAAGAAG ATATGCAGGA AGAAGAAGAA GAATATATGG ATCAAGAAGC ACCGGATTTG
    TCTGGGTCTG ATTTAGACGA TTATCGTCTG CCATTGTCTG GGTCTGATTC AGATGATTAT
    TGTTGTTATA TGGCTGACTT TGAATTGGCA AAAAGCAAGA AAAAAGCAAA ACGTGGACCT
    ATAGAAACAG AAGAGGCACA ACCTTTCAGA CGTATGAGAC GTGTAACTAC AATGTGTCAT
    ATGAGTCCTC CCCGATCAGA GGCACAACCT TCTGAACATG ATGGAGAGCG AGAATGGCGG
    ACGTTACATG CCGAGCAGGA AGTGGAGCGC ATAGGTGGAA GGCATGGTGG ACCGCATATC
    GATCGAGGGA ACAGGAGAGG CGCATGGATG CCAGAGCGAA CGGAGCCTGC GATGGTAGAA
    GCTGCCATGC CAATGCGCCA TATGGATCGT TGCGGAACAG AGGCACAACC TTCTGAACAT
    GTGATGCAGG AAAGAGCTGC CAAGCCAATG TGTCTTATGA GTCTTCCCCG ATCAGTGATG
    CAGGAAAGAG CTGCCGAGGC AATGCGCCAT TTGGGTTTTC GCCGAACAGA AATCATGGAC
    ATGCTGTCTC CAATGCCTCC ATCCAACGCT CCAATCTCTC CATCATATTG TGAAAAGCGC
    AAGGTTCACG CTGACATGCT AGAAATCATG GACATGCCGG CTCCAACCTC TCCATCATAT
    GCTCCAACCT CTCCATCATA TCTTCAAAGG CGCAAGAGCT TTTCTCTTAT GGGCGCAGCT
    CCAAGCTCTG CTGTTTCGTA TGCAGCTCCA ACTTTTGGTT CTGCAACTGC AGGGTTTACA
    AGCTCTGCTG TTTCGGATGC AGCTCCAGCT TTTGGCTCTG CAGCTGCAAG ACCTCCATTA
    TTTGGTTATA AAAGCTCTGC TGTTTCGGAT GCAGCTCCAG GTTTCGGCAC AGTGACTGCT
    GCTCAGAGCT CTGGCTTTTT TTGTTCAGCA GGTTTCAGAT TTGGAGCTCT AGTTCCACCT
    CCACCTCCGA TACCTGGCGG GGTTTGCAGA TTTGCACCGC CACCTCCACC TCCAGTTCCG
    GATCCACTTC TGATGGCTAC TGGCAGGGTT TGCAGATTTG CACCGCCACC TCCACCTCCA
    GTTCCGGATC CACTTCTGAT GGCTACTGGC AGGCTTCGGA TACCTGCTGG CGGGGTTTTA
    AGCTTTGCAC CTCCACCTCC ACCTCCAGCT CCAGTTCCGG ATCCACCTCC GATACCTGTT
    GACAGGCTTC TGATGGCTAC TGGCAAGCCT CGGAAACATG CTGGCAGGGT CAGAGCCAAG
    GCTTTGTTCG CTCTAGCTCC TAGTGCTCCA ACTTCAGACT CCGAAGTTAA AGCAAGAATT
    GCTGCTAAAA TAAGAAGAGC CCCTCCATCT TTCGTCCCCC TTTCAGCTCT ACAGCATGAG
    GAGGGATACT GGCTGCTGGA TGAAAAGCTC GGATCTCTAT TGAACATTAA TGTCAATTAC
    TTGATAGATG TGTTTTTATC CAAGAACGGG ATTCAGTCTC TTGGGAGGAA AGGAAAAGAA
    GAGGTGCTGG CACTTATTGC GACCCTCTTG GTGCTGCAAA TGATTCGCAC GTATAAGTTG
    GTGGACATAA CATTTAAGAG TCTGATGAAA CTGGACGAAT CATTTTGCAC AAGCCCCTTT
    TACCAGACAA TTGAGAAATC CGTCAAATGG GCACGAAAAA AAGATGAACA ATATCCTGGC
    ATCTGTTCTC GACTTGGATT GGGCAAGGAT TGGGAATCTG CCACCCGGAA GTTATTATCC
    ATTGATCCAG TGGATCTTTC CTCTGATCTC TATCCTGCTG TTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753139.1
    CDS135..5642
    Translation

    Target ORF information:

    RefSeq Version XM_031897279.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897279.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGATCCT GAGAAAACTG TCTTCCCTAA AAAAGTTCCA
    TCCCTGCAAG ACAATGATGA GTTCATCAAC AGGGATTACA GTGAATATGA TGCAGACACC
    CTCCCCGACC CTCCAGATGC CGAAGTAGCA AAATACTGTT TTTTTCAAAA TGGTCCCATT
    GTGGCAGTTC TGGAGCTGTT GTGCATTCCT GAATCCTCTT CATTCCTATT TCAAATATCT
    AAAACAATTT GTAATTCCAA TGGATCGGAG CCTGACAGCT CCTATATGTT CAAACACACA
    GCGGAGGCGG CTTGTAACAA GTATGAGAAG TGCACTGAGG ATTTAGAAAA AGAAGGCTTT
    AAGAGAGCAA AGCGTATCCC ACCAAAGGTT GCCCTGCACA CATCTGAGGC TCTGCAGAAG
    GTCCTGCTTG AAGAAGCCAT AAATGCTACC AAGTTATCTC CAGATGTTGG ATATTTTGTG
    GAAAGTGTTT GGATTGAGGC TCTTGGCCAT TTGGATAACA TCCTCGCGCG TCCCGTAAAC
    AGCATCAGTC TGAATGATGT AAGCAAAGCA GAGGGTGTTC TGCTCCAGGT TAGGAAAGCT
    TTGGACACAG GAACAGAACC TGGAGACATA AGGAAAGCAA TGGCTGAGTT TTATCGGCTC
    ATTCCCCATA AGAAACCTCT GCTGGCCAAC ATTGATAAGA AACTTTTGTC AACAAAGCAA
    GATCTGTGCC AGCTTATTAG AGATATGGTG AGTGTATGTG AAACCAACTC ATCGAAGCCA
    AACCCAAGCA GCCTTGCCAA ATACCAGGCC CTGCGATGCC AAATAGAGCA TGTGGAGCCC
    AATACTGAGG AATTTCATCA GGTGTCACAG CAAATCGTGG AGAACAAACA CTGTGAGGAG
    TCGATTAACA TACTGAAAAT ATTCCGCGTT GGGAAATTAA GTGAAGCCAC TGGGTTTCAA
    AAGAATCTTG GGAATGTCAA GTCCCTGCTC CATGCATCTT CCCCACGTCA CTTTGTGGGG
    ATCCTTTCCC GTGGACTGCT CTTGCCAAAA AATGTGGAAG ATGATTTGGG TTTAAAAAGA
    ACTGATATTG GAAATCTTGG AAGTGGAATT TACTTCAGTG ATTCCATAAG TACAAGTGTT
    AAATATTCGG AACCCAGTGT AACCAATGGG GCGCGTCTCC TGTTGGTTTG TGAGGTGGCT
    CTGGGGAACT GCAAGGATCT CTACAAAAGA GACTTCTCCC TTACTGGAGC TCCAAGTGGC
    TTTAACAGCG TTCATGGTGT CCGACAAATG CCAGGGAGGA AATCTGATTT TGAGGATGAT
    GAATTTGTGG TATATAAAAA GGATCAAATA AAAATGAGAT ACGCCGTGCA GTTTTGCACT
    GATAAAGACC AAGTCAACAT GGAACGCGAC AAACTTTCTG TTCAGTTTGA GCCCATGGAG
    GAAGATGTTG GAGAAGAACC AGTGCAGGTG GAAGATCTCA TGTTGGAGAA TCTTCCGGAA
    CCACAGAGCA TAAATGGCGG CCTCCAGAGT GTTCATGGCA ACCAGATCCC TTTGGAAAAT
    ATTCATGTGA AAGGCAAAAT GATGGATTTA GTTGCAGAGG TTATATTATT CCAGACCTAC
    ATAAACAAAT CCTCTGTTCC CATCGAGGCT AAATATGTTT TTCCCCTGGA CGGCACAGCA
    GCCGTGTGTG GCTTTGAAGC ATTTATAAAT GGAAAACACG TTATCGGAGA GGTTAAAGAG
    AAGGAGCAAG CGCATAAGGA GTACAGGGCT GCTATCAGCG AGGGACATGG GGCTTACCTT
    ATGGACCAGG ACGCTGCTGA CGTCTTCACT GTAAGTGTTG GAAACCTGCC CCCAAACGCC
    ACTGTAATCA TTAAAATCAC GTACATAACG GAATTGATTC TGGAGTATGG ATCAGTGATA
    TTCAACATTC CTGGCACAGT GGCATCTTGG CAAAAGGATA AAGCTCTGAA AGAAAATACT
    CAGGATACAG TAGAAAAAGT CTGTGTTGAA GAAAGTGGTG TGCCACAAGG TTGTTTCTCT
    CTGGAGATGT CTGTTGAGAT GTCTTTTAAA ATCGAGAATG TGTTAAGTTT CACTCACAGT
    ATTTCAACGA AAAAAACTGA TTGCAAAGCT GTGATTGCAA CTAAAAAAGA CAGTTCATTG
    GATGACGAGG GGTTTCAGTT GAGAGTCTAC CTGGAATCAA GCTTCTTGGA GTCTTCTTGG
    AACCAACCGA GAATGTGGGT GGAAAAACAC CCCAAAGAAG ACAGTGAGGC ATGCATGCTT
    GTGTTCCTGC CTAGTTTTAA GACAAGCATA CAATCCTGGG ATTTAACCAT CTTACTTGAT
    TGCTCCAATT CTATGGAATC CACTTTCCAA AGCGCAAAAC GGATTGCTCT GCTTGCAGCC
    AGCTCGTTAA ACCCATGGCA CAACATAAAC ATAATCAGCT TTGGTACAGG TCATAAGGAA
    TTTAGCATCC GTCCAAAAGA GAGCCAGAAT TTAATACCGG AGCTGGAGCA GTTTATTAAA
    ATGGCCAAAC CAAACATGGG AAACACAGAA CTTTGGAAGC CTCTGCAGTC CTTATGTTTG
    CTGGCTCCTC CCAGTGACAT GCACAATGTT CTTCTTATAT CTGATGGACA CATCCAGAAT
    GAGAGCCTCG TCTTTCAGAT CCTCAAAAAG AATGCAGGGA AAGTGCGGCT GTTCACCTGT
    GGCGTTGGTG CAACAGCCAA TCGCCATATG TTGCGGTGTT TGGCTCAGTA CGGTGCTGGG
    TTCTTTGAAT TTTTTGAAGA TAAATCCAAG TCCAGCTGGA AAAAAAAGAT GGAGGCACAG
    CTGGAAAGAA TGGATTCCCC TGCATGCACT AGTGCATCTG TTAAATGGAT GCAGTTCAGT
    GATAATGACC CAGAGCCCGT TCAAGCCCCA GCACACATAC CGGCTTTGTT CAACCGCAGT
    TTCCTTCTTG TTTATGGCTT TGCTCCTCGT TGCACTCAGG CCAAGCTACA AGCTTTAATT
    GATGACAATG AACTGGACAC TATGGTATCA ACTACAGAAT TACAGAAGAC CAGAGGAACG
    ATGCTTCATA AACTGACGGC AAGGGCTGTT ATTAAGGATT ATGAAGATGG GATCCTGCAC
    GAGAAAGAAC ATGAACATGA GATGAAAAAG CAGCAAATGA AGTCCTTTAT TATTGAGCTG
    AGCAAGAAGT ACTCAATCGT CACTCAGTTT ACAAGCTTTG TCGCTGTAGA GAAACGGGAT
    GCACAAGAGA AGCCAAAGGA CTATGTACCA AATGTGCTTG AAATAATATC CACAGAAGAT
    GTTGATATAT TACCATACAT GATGTGGGAA ACACAGGAAG AGGATCAGGC CGCTGTGCTG
    CAGGAAGAAG ATATGCAGGA AGAAGAAGAA GAATATATGG ATCAAGAAGC ACCGGATTTG
    TCTGGGTCTG ATTTAGACGA TTATCGTCTG CCATTGTCTG GGTCTGATTC AGATGATTAT
    TGTTGTTATA TGGCTGACTT TGAATTGGCA AAAAGCAAGA AAAAAGCAAA ACGTGGACCT
    ATAGAAACAG AAGAGGCACA ACCTTTCAGA CGTATGAGAC GTGTAACTAC AATGTGTCAT
    ATGAGTCCTC CCCGATCAGA GGCACAACCT TCTGAACATG ATGGAGAGCG AGAATGGCGG
    ACGTTACATG CCGAGCAGGA AGTGGAGCGC ATAGGTGGAA GGCATGGTGG ACCGCATATC
    GATCGAGGGA ACAGGAGAGG CGCATGGATG CCAGAGCGAA CGGAGCCTGC GATGGTAGAA
    GCTGCCATGC CAATGCGCCA TATGGATCGT TGCGGAACAG AGGCACAACC TTCTGAACAT
    GTGATGCAGG AAAGAGCTGC CAAGCCAATG TGTCTTATGA GTCTTCCCCG ATCAGTGATG
    CAGGAAAGAG CTGCCGAGGC AATGCGCCAT TTGGGTTTTC GCCGAACAGA AATCATGGAC
    ATGCTGTCTC CAATGCCTCC ATCCAACGCT CCAATCTCTC CATCATATTG TGAAAAGCGC
    AAGGTTCACG CTGACATGCT AGAAATCATG GACATGCCGG CTCCAACCTC TCCATCATAT
    GCTCCAACCT CTCCATCATA TCTTCAAAGG CGCAAGAGCT TTTCTCTTAT GGGCGCAGCT
    CCAAGCTCTG CTGTTTCGTA TGCAGCTCCA ACTTTTGGTT CTGCAACTGC AGGGTTTACA
    AGCTCTGCTG TTTCGGATGC AGCTCCAGCT TTTGGCTCTG CAGCTGCAAG ACCTCCATTA
    TTTGGTTATA AAAGCTCTGC TGTTTCGGAT GCAGCTCCAG GTTTCGGCAC AGTGACTGCT
    GCTCAGAGCT CTGGCTTTTT TTGTTCAGCA GGTTTCAGAT TTGGAGCTCT AGTTCCACCT
    CCACCTCCGA TACCTGGCGG GGTTTGCAGA TTTGCACCGC CACCTCCACC TCCAGTTCCG
    GATCCACTTC TGATGGCTAC TGGCAGGGTT TGCAGATTTG CACCGCCACC TCCACCTCCA
    GTTCCGGATC CACTTCTGAT GGCTACTGGC AGGCTTCGGA TACCTGCTGG CGGGGTTTTA
    AGCTTTGCAC CTCCACCTCC ACCTCCAGCT CCAGTTCCGG ATCCACCTCC GATACCTGTT
    GACAGGCTTC TGATGGCTAC TGGCAAGCCT CGGAAACATG CTGGCAGGGT CAGAGCCAAG
    GCTTTGTTCG CTCTAGCTCC TAGTGCTCCA ACTTCAGACT CCGAAGTTAA AGCAAGAATT
    GCTGCTAAAA TAAGAAGAGC CCCTCCATCT TTCGTCCCCC TTTCAGCTCT ACAGCATGAG
    GAGGGATACT GGCTGCTGGA TGAAAAGCTC GGATCTCTAT TGAACATTAA TGTCAATTAC
    TTGATAGATG TGTTTTTATC CAAGAACGGG ATTCAGTCTC TTGGGAGGAA AGGAAAAGAA
    GAGGTGCTGG CACTTATTGC GACCCTCTTG GTGCTGCAAA TGATTCGCAC GTATAAGTTG
    GTGGACATAA CATTTAAGAG TCTGATGAAA CTGGACGAAT CATTTTGCAC AAGCCCCTTT
    TACCAGACAA TTGAGAAATC CGTCAAATGG GCACGAAAAA AAGATGAACA ATATCCTGGC
    ATCTGTTCTC GACTTGGATT GGGCAAGGAT TGGGAATCTG CCACCCGGAA GTTATTATCC
    ATTGATCCAG TGGATCTTTC CTCTGATCTC TATCCTGCTG TTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52865
    Clone ID Related Accession (Same CDS sequence) XM_031897280.1
    Accession Version XM_031897280.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGATG
    CGCCATATGG ATCGTTGCGG AACAGAGGCA CAACCTTCTG AACATGTGAT GCAGGAAAGA
    GCTGCCAAGC CAATGTGTCT TATGAGTCTT CCCCGATCAG TGATGCAGGA AAGAGCTGCC
    GAGGCAATGC GCCATTTGGG TTTTCGCCGA ACAGAAATCA TGGACATGCT GTCTCCAATG
    CCTCCATCCA ACGCTCCAAT CTCTCCATCA TATTGTGAAA AGCGCAAGGT TCACGCTGAC
    ATGCTAGAAA TCATGGACAT GCCGGCTCCA ACCTCTCCAT CATATGCTCC AACCTCTCCA
    TCATATCTTC AAAGGCGCAA GAGCTTTTCT CTTATGGGCG CAGCTCCAAG CTCTGCTGTT
    TCGTATGCAG CTCCAACTTT TGGTTCTGCA ACTGCAGGGT TTACAAGCTC TGCTGTTTCG
    GATGCAGCTC CAGCTTTTGG CTCTGCAGCT GCAAGACCTC CATTATTTGG TTATAAAAGC
    TCTGCTGTTT CGGATGCAGC TCCAGGTTTC GGCACAGTGA CTGCTGCTCA GAGCTCTGGC
    TTTTTTTGTT CAGCAGGTTT CAGATTTGGA GCTCTAGTTC CACCTCCACC TCCGATACCT
    GGCGGGGTTT GCAGATTTGC ACCGCCACCT CCACCTCCAG TTCCGGATCC ACTTCTGATG
    GCTACTGGCA GGGTTTGCAG ATTTGCACCG CCACCTCCAC CTCCAGTTCC GGATCCACTT
    CTGATGGCTA CTGGCAGGCT TCGGATACCT GCTGGCGGGG TTTTAAGCTT TGCACCTCCA
    CCTCCACCTC CAGCTCCAGT TCCGGATCCA CCTCCGATAC CTGTTGACAG GCTTCTGATG
    GCTACTGGCA AGCCTCGGAA ACATGCTGGC AGGGTCAGAG CCAAGGCTTT GTTCGCTCTA
    GCTCCTAGTG CTCCAACTTC AGACTCCGAA GTTAAAGCAA GAATTGCTGC TAAAATAAGA
    AGAGCCCCTC CATCTTTCGT CCCCCTTTCA GCTCTACAGC ATGAGGAGGG ATACTGGCTG
    CTGGATGAAA AGCTCGGATC TCTATTGAAC ATTAATGTCA ATTACTTGAT AGATGTGTTT
    TTATCCAAGA ACGGGATTCA GTCTCTTGGG AGGAAAGGAA AAGAAGAGGT GCTGGCACTT
    ATTGCGACCC TCTTGGTGCT GCAAATGATT CGCACGTATA AGTTGGTGGA CATAACATTT
    AAGAGTCTGA TGAAACTGGA CGAATCATTT TGCACAAGCC CCTTTTACCA GACAATTGAG
    AAATCCGTCA AATGGGCACG AAAAAAAGAT GAACAATATC CTGGCATCTG TTCTCGACTT
    GGATTGGGCA AGGATTGGGA ATCTGCCACC CGGAAGTTAT TATCCATTGA TCCAGTGGAT
    CTTTCCTCTG ATCTCTATCC TGCTGTTGTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753140.1
    CDS135..5627
    Translation

    Target ORF information:

    RefSeq Version XM_031897280.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897280.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGATG
    CGCCATATGG ATCGTTGCGG AACAGAGGCA CAACCTTCTG AACATGTGAT GCAGGAAAGA
    GCTGCCAAGC CAATGTGTCT TATGAGTCTT CCCCGATCAG TGATGCAGGA AAGAGCTGCC
    GAGGCAATGC GCCATTTGGG TTTTCGCCGA ACAGAAATCA TGGACATGCT GTCTCCAATG
    CCTCCATCCA ACGCTCCAAT CTCTCCATCA TATTGTGAAA AGCGCAAGGT TCACGCTGAC
    ATGCTAGAAA TCATGGACAT GCCGGCTCCA ACCTCTCCAT CATATGCTCC AACCTCTCCA
    TCATATCTTC AAAGGCGCAA GAGCTTTTCT CTTATGGGCG CAGCTCCAAG CTCTGCTGTT
    TCGTATGCAG CTCCAACTTT TGGTTCTGCA ACTGCAGGGT TTACAAGCTC TGCTGTTTCG
    GATGCAGCTC CAGCTTTTGG CTCTGCAGCT GCAAGACCTC CATTATTTGG TTATAAAAGC
    TCTGCTGTTT CGGATGCAGC TCCAGGTTTC GGCACAGTGA CTGCTGCTCA GAGCTCTGGC
    TTTTTTTGTT CAGCAGGTTT CAGATTTGGA GCTCTAGTTC CACCTCCACC TCCGATACCT
    GGCGGGGTTT GCAGATTTGC ACCGCCACCT CCACCTCCAG TTCCGGATCC ACTTCTGATG
    GCTACTGGCA GGGTTTGCAG ATTTGCACCG CCACCTCCAC CTCCAGTTCC GGATCCACTT
    CTGATGGCTA CTGGCAGGCT TCGGATACCT GCTGGCGGGG TTTTAAGCTT TGCACCTCCA
    CCTCCACCTC CAGCTCCAGT TCCGGATCCA CCTCCGATAC CTGTTGACAG GCTTCTGATG
    GCTACTGGCA AGCCTCGGAA ACATGCTGGC AGGGTCAGAG CCAAGGCTTT GTTCGCTCTA
    GCTCCTAGTG CTCCAACTTC AGACTCCGAA GTTAAAGCAA GAATTGCTGC TAAAATAAGA
    AGAGCCCCTC CATCTTTCGT CCCCCTTTCA GCTCTACAGC ATGAGGAGGG ATACTGGCTG
    CTGGATGAAA AGCTCGGATC TCTATTGAAC ATTAATGTCA ATTACTTGAT AGATGTGTTT
    TTATCCAAGA ACGGGATTCA GTCTCTTGGG AGGAAAGGAA AAGAAGAGGT GCTGGCACTT
    ATTGCGACCC TCTTGGTGCT GCAAATGATT CGCACGTATA AGTTGGTGGA CATAACATTT
    AAGAGTCTGA TGAAACTGGA CGAATCATTT TGCACAAGCC CCTTTTACCA GACAATTGAG
    AAATCCGTCA AATGGGCACG AAAAAAAGAT GAACAATATC CTGGCATCTG TTCTCGACTT
    GGATTGGGCA AGGATTGGGA ATCTGCCACC CGGAAGTTAT TATCCATTGA TCCAGTGGAT
    CTTTCCTCTG ATCTCTATCC TGCTGTTGTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52866
    Clone ID Related Accession (Same CDS sequence) XM_031897281.1
    Accession Version XM_031897281.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACC TCCGATACCT
    GTTGACAGGC TTCTGATGGC TACTGGCAAG CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC
    AAGGCTTTGT TCGCTCTAGC TCCTAGTGCT CCAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753141.1
    CDS135..5624
    Translation

    Target ORF information:

    RefSeq Version XM_031897281.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897281.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACC TCCGATACCT
    GTTGACAGGC TTCTGATGGC TACTGGCAAG CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC
    AAGGCTTTGT TCGCTCTAGC TCCTAGTGCT CCAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52867
    Clone ID Related Accession (Same CDS sequence) XM_031897282.1
    Accession Version XM_031897282.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCG GATACCTGCT GGCGGGGTTT TAAGCTTTGC ACCTCCACCT
    CCACCTCCAG CTCCAGTTCC GGATCCACCT CCGATACCTG TTGACAGGCT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT
    CCTAGTGCTC CAACTTCAGA CTCCGAAGTT AAAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753142.1
    CDS135..5624
    Translation

    Target ORF information:

    RefSeq Version XM_031897282.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897282.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCG GATACCTGCT GGCGGGGTTT TAAGCTTTGC ACCTCCACCT
    CCACCTCCAG CTCCAGTTCC GGATCCACCT CCGATACCTG TTGACAGGCT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT
    CCTAGTGCTC CAACTTCAGA CTCCGAAGTT AAAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52868
    Clone ID Related Accession (Same CDS sequence) XM_031897283.1
    Accession Version XM_031897283.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAG TTTGCAGATT TGCACCGCCA CCTCCACCTC CAGTTCCGGA TCCACTTCTG
    ATGGCTACTG GCAGGCTTCG GATACCTGCT GGCGGGGTTT TAAGCTTTGC ACCTCCACCT
    CCACCTCCAG CTCCAGTTCC GGATCCACCT CCGATACCTG TTGACAGGCT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT
    CCTAGTGCTC CAACTTCAGA CTCCGAAGTT AAAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753143.1
    CDS135..5624
    Translation

    Target ORF information:

    RefSeq Version XM_031897283.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897283.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAG TTTGCAGATT TGCACCGCCA CCTCCACCTC CAGTTCCGGA TCCACTTCTG
    ATGGCTACTG GCAGGCTTCG GATACCTGCT GGCGGGGTTT TAAGCTTTGC ACCTCCACCT
    CCACCTCCAG CTCCAGTTCC GGATCCACCT CCGATACCTG TTGACAGGCT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT
    CCTAGTGCTC CAACTTCAGA CTCCGAAGTT AAAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52869
    Clone ID Related Accession (Same CDS sequence) XM_031897284.1
    Accession Version XM_031897284.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC TGTGAAAAGC GCAAGGTTCA CGCTGACATG
    CTAGAAATCA TGGACATGCC GGCTCCAACC TCTCCATCAT ATGCTCCAAC CTCTCCATCA
    TATCTTCAAA GGCGCAAGAG CTTTTCTCTT ATGGGCGCAG CTCCAAGCTC TGCTGTTTCG
    TATGCAGCTC CAACTTTTGG TTCTGCAACT GCAGGGTTTA CAAGCTCTGC TGTTTCGGAT
    GCAGCTCCAG CTTTTGGCTC TGCAGCTGCA AGACCTCCAT TATTTGGTTA TAAAAGCTCT
    GCTGTTTCGG ATGCAGCTCC AGGTTTCGGC ACAGTGACTG CTGCTCAGAG CTCTGGCTTT
    TTTTGTTCAG CAGGTTTCAG ATTTGGAGCT CTAGTTCCAC CTCCACCTCC GATACCTGGC
    GGGGTTTGCA GATTTGCACC GCCACCTCCA CCTCCAGTTC CGGATCCACT TCTGATGGCT
    ACTGGCAGGG TTTGCAGATT TGCACCGCCA CCTCCACCTC CAGTTCCGGA TCCACTTCTG
    ATGGCTACTG GCAGGCTTCG GATACCTGCT GGCGGGGTTT TAAGCTTTGC ACCTCCACCT
    CCACCTCCAG CTCCAGTTCC GGATCCACCT CCGATACCTG TTGACAGGCT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT
    CCTAGTGCTC CAACTTCAGA CTCCGAAGTT AAAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753144.1
    CDS135..5624
    Translation

    Target ORF information:

    RefSeq Version XM_031897284.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897284.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC TGTGAAAAGC GCAAGGTTCA CGCTGACATG
    CTAGAAATCA TGGACATGCC GGCTCCAACC TCTCCATCAT ATGCTCCAAC CTCTCCATCA
    TATCTTCAAA GGCGCAAGAG CTTTTCTCTT ATGGGCGCAG CTCCAAGCTC TGCTGTTTCG
    TATGCAGCTC CAACTTTTGG TTCTGCAACT GCAGGGTTTA CAAGCTCTGC TGTTTCGGAT
    GCAGCTCCAG CTTTTGGCTC TGCAGCTGCA AGACCTCCAT TATTTGGTTA TAAAAGCTCT
    GCTGTTTCGG ATGCAGCTCC AGGTTTCGGC ACAGTGACTG CTGCTCAGAG CTCTGGCTTT
    TTTTGTTCAG CAGGTTTCAG ATTTGGAGCT CTAGTTCCAC CTCCACCTCC GATACCTGGC
    GGGGTTTGCA GATTTGCACC GCCACCTCCA CCTCCAGTTC CGGATCCACT TCTGATGGCT
    ACTGGCAGGG TTTGCAGATT TGCACCGCCA CCTCCACCTC CAGTTCCGGA TCCACTTCTG
    ATGGCTACTG GCAGGCTTCG GATACCTGCT GGCGGGGTTT TAAGCTTTGC ACCTCCACCT
    CCACCTCCAG CTCCAGTTCC GGATCCACCT CCGATACCTG TTGACAGGCT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT
    CCTAGTGCTC CAACTTCAGA CTCCGAAGTT AAAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52870
    Clone ID Related Accession (Same CDS sequence) XM_031897285.1
    Accession Version XM_031897285.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT
    CCTAGTGCTC CAACTTCAGA CTCCGAAGTT AAAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753145.1
    CDS135..5624
    Translation

    Target ORF information:

    RefSeq Version XM_031897285.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897285.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT
    CCTAGTGCTC CAACTTCAGA CTCCGAAGTT AAAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52871
    Clone ID Related Accession (Same CDS sequence) XM_031897286.1
    Accession Version XM_031897286.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGG CTCCAATCTC TCCATCATAT TGTGAAAAGC GCAAGGTTCA CGCTGACATG
    CTAGAAATCA TGGACATGCC GGCTCCAACC TCTCCATCAT ATGCTCCAAC CTCTCCATCA
    TATCTTCAAA GGCGCAAGAG CTTTTCTCTT ATGGGCGCAG CTCCAAGCTC TGCTGTTTCG
    TATGCAGCTC CAACTTTTGG TTCTGCAACT GCAGGGTTTA CAAGCTCTGC TGTTTCGGAT
    GCAGCTCCAG CTTTTGGCTC TGCAGCTGCA AGACCTCCAT TATTTGGTTA TAAAAGCTCT
    GCTGTTTCGG ATGCAGCTCC AGGTTTCGGC ACAGTGACTG CTGCTCAGAG CTCTGGCTTT
    TTTTGTTCAG CAGGTTTCAG ATTTGGAGCT CTAGTTCCAC CTCCACCTCC GATACCTGGC
    GGGGTTTGCA GATTTGCACC GCCACCTCCA CCTCCAGTTC CGGATCCACT TCTGATGGCT
    ACTGGCAGGG TTTGCAGATT TGCACCGCCA CCTCCACCTC CAGTTCCGGA TCCACTTCTG
    ATGGCTACTG GCAGGCTTCG GATACCTGCT GGCGGGGTTT TAAGCTTTGC ACCTCCACCT
    CCACCTCCAG CTCCAGTTCC GGATCCACCT CCGATACCTG TTGACAGGCT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT
    CCTAGTGCTC CAACTTCAGA CTCCGAAGTT AAAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753146.1
    CDS135..5624
    Translation

    Target ORF information:

    RefSeq Version XM_031897286.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897286.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGG CTCCAATCTC TCCATCATAT TGTGAAAAGC GCAAGGTTCA CGCTGACATG
    CTAGAAATCA TGGACATGCC GGCTCCAACC TCTCCATCAT ATGCTCCAAC CTCTCCATCA
    TATCTTCAAA GGCGCAAGAG CTTTTCTCTT ATGGGCGCAG CTCCAAGCTC TGCTGTTTCG
    TATGCAGCTC CAACTTTTGG TTCTGCAACT GCAGGGTTTA CAAGCTCTGC TGTTTCGGAT
    GCAGCTCCAG CTTTTGGCTC TGCAGCTGCA AGACCTCCAT TATTTGGTTA TAAAAGCTCT
    GCTGTTTCGG ATGCAGCTCC AGGTTTCGGC ACAGTGACTG CTGCTCAGAG CTCTGGCTTT
    TTTTGTTCAG CAGGTTTCAG ATTTGGAGCT CTAGTTCCAC CTCCACCTCC GATACCTGGC
    GGGGTTTGCA GATTTGCACC GCCACCTCCA CCTCCAGTTC CGGATCCACT TCTGATGGCT
    ACTGGCAGGG TTTGCAGATT TGCACCGCCA CCTCCACCTC CAGTTCCGGA TCCACTTCTG
    ATGGCTACTG GCAGGCTTCG GATACCTGCT GGCGGGGTTT TAAGCTTTGC ACCTCCACCT
    CCACCTCCAG CTCCAGTTCC GGATCCACCT CCGATACCTG TTGACAGGCT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT
    CCTAGTGCTC CAACTTCAGA CTCCGAAGTT AAAGCAAGAA TTGCTGCTAA AATAAGAAGA
    GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT CTACAGCATG AGGAGGGATA CTGGCTGCTG
    GATGAAAAGC TCGGATCTCT ATTGAACATT AATGTCAATT ACTTGATAGA TGTGTTTTTA
    TCCAAGAACG GGATTCAGTC TCTTGGGAGG AAAGGAAAAG AAGAGGTGCT GGCACTTATT
    GCGACCCTCT TGGTGCTGCA AATGATTCGC ACGTATAAGT TGGTGGACAT AACATTTAAG
    AGTCTGATGA AACTGGACGA ATCATTTTGC ACAAGCCCCT TTTACCAGAC AATTGAGAAA
    TCCGTCAAAT GGGCACGAAA AAAAGATGAA CAATATCCTG GCATCTGTTC TCGACTTGGA
    TTGGGCAAGG ATTGGGAATC TGCCACCCGG AAGTTATTAT CCATTGATCC AGTGGATCTT
    TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52872
    Clone ID Related Accession (Same CDS sequence) XM_031897287.1
    Accession Version XM_031897287.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5481bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAAGCTCTG CTGTTTCGGA TGCAGCTCCA
    GCTTTTGGCT CTGCAGCTGC AAGACCTCCA TTATTTGGTT ATAAAAGCTC TGCTGTTTCG
    GATGCAGCTC CAGGTTTCGG CACAGTGACT GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA
    GCAGGTTTCA GATTTGGAGC TCTAGTTCCA CCTCCACCTC CGATACCTGG CGGGGTTTGC
    AGATTTGCAC CGCCACCTCC ACCTCCAGTT CCGGATCCAC TTCTGATGGC TACTGGCAGG
    GTTTGCAGAT TTGCACCGCC ACCTCCACCT CCAGTTCCGG ATCCACTTCT GATGGCTACT
    GGCAGGCTTC GGATACCTGC TGGCGGGGTT TTAAGCTTTG CACCTCCACC TCCACCTCCA
    GCTCCAGTTC CGGATCCACC TCCGATACCT GTTGACAGGC TTCTGATGGC TACTGGCAAG
    CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC AAGGCTTTGT TCGCTCTAGC TCCTAGTGCT
    CCAACTTCAG ACTCCGAAGT TAAAGCAAGA ATTGCTGCTA AAATAAGAAG AGCCCCTCCA
    TCTTTCGTCC CCCTTTCAGC TCTACAGCAT GAGGAGGGAT ACTGGCTGCT GGATGAAAAG
    CTCGGATCTC TATTGAACAT TAATGTCAAT TACTTGATAG ATGTGTTTTT ATCCAAGAAC
    GGGATTCAGT CTCTTGGGAG GAAAGGAAAA GAAGAGGTGC TGGCACTTAT TGCGACCCTC
    TTGGTGCTGC AAATGATTCG CACGTATAAG TTGGTGGACA TAACATTTAA GAGTCTGATG
    AAACTGGACG AATCATTTTG CACAAGCCCC TTTTACCAGA CAATTGAGAA ATCCGTCAAA
    TGGGCACGAA AAAAAGATGA ACAATATCCT GGCATCTGTT CTCGACTTGG ATTGGGCAAG
    GATTGGGAAT CTGCCACCCG GAAGTTATTA TCCATTGATC CAGTGGATCT TTCCTCTGAT
    CTCTATCCTG CTGTTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753147.1
    CDS135..5615
    Translation

    Target ORF information:

    RefSeq Version XM_031897287.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAAGCTCTG CTGTTTCGGA TGCAGCTCCA
    GCTTTTGGCT CTGCAGCTGC AAGACCTCCA TTATTTGGTT ATAAAAGCTC TGCTGTTTCG
    GATGCAGCTC CAGGTTTCGG CACAGTGACT GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA
    GCAGGTTTCA GATTTGGAGC TCTAGTTCCA CCTCCACCTC CGATACCTGG CGGGGTTTGC
    AGATTTGCAC CGCCACCTCC ACCTCCAGTT CCGGATCCAC TTCTGATGGC TACTGGCAGG
    GTTTGCAGAT TTGCACCGCC ACCTCCACCT CCAGTTCCGG ATCCACTTCT GATGGCTACT
    GGCAGGCTTC GGATACCTGC TGGCGGGGTT TTAAGCTTTG CACCTCCACC TCCACCTCCA
    GCTCCAGTTC CGGATCCACC TCCGATACCT GTTGACAGGC TTCTGATGGC TACTGGCAAG
    CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC AAGGCTTTGT TCGCTCTAGC TCCTAGTGCT
    CCAACTTCAG ACTCCGAAGT TAAAGCAAGA ATTGCTGCTA AAATAAGAAG AGCCCCTCCA
    TCTTTCGTCC CCCTTTCAGC TCTACAGCAT GAGGAGGGAT ACTGGCTGCT GGATGAAAAG
    CTCGGATCTC TATTGAACAT TAATGTCAAT TACTTGATAG ATGTGTTTTT ATCCAAGAAC
    GGGATTCAGT CTCTTGGGAG GAAAGGAAAA GAAGAGGTGC TGGCACTTAT TGCGACCCTC
    TTGGTGCTGC AAATGATTCG CACGTATAAG TTGGTGGACA TAACATTTAA GAGTCTGATG
    AAACTGGACG AATCATTTTG CACAAGCCCC TTTTACCAGA CAATTGAGAA ATCCGTCAAA
    TGGGCACGAA AAAAAGATGA ACAATATCCT GGCATCTGTT CTCGACTTGG ATTGGGCAAG
    GATTGGGAAT CTGCCACCCG GAAGTTATTA TCCATTGATC CAGTGGATCT TTCCTCTGAT
    CTCTATCCTG CTGTTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52873
    Clone ID Related Accession (Same CDS sequence) XM_031897288.1
    Accession Version XM_031897288.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5481bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACC TCCGATACCT
    GTTGACAGGC TTCTGATGGC TACTGGCAAG CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC
    AAGACTTCAG ACTCCGAAGT TAAAGCAAGA ATTGCTGCTA AAATAAGAAG AGCCCCTCCA
    TCTTTCGTCC CCCTTTCAGC TCTACAGCAT GAGGAGGGAT ACTGGCTGCT GGATGAAAAG
    CTCGGATCTC TATTGAACAT TAATGTCAAT TACTTGATAG ATGTGTTTTT ATCCAAGAAC
    GGGATTCAGT CTCTTGGGAG GAAAGGAAAA GAAGAGGTGC TGGCACTTAT TGCGACCCTC
    TTGGTGCTGC AAATGATTCG CACGTATAAG TTGGTGGACA TAACATTTAA GAGTCTGATG
    AAACTGGACG AATCATTTTG CACAAGCCCC TTTTACCAGA CAATTGAGAA ATCCGTCAAA
    TGGGCACGAA AAAAAGATGA ACAATATCCT GGCATCTGTT CTCGACTTGG ATTGGGCAAG
    GATTGGGAAT CTGCCACCCG GAAGTTATTA TCCATTGATC CAGTGGATCT TTCCTCTGAT
    CTCTATCCTG CTGTTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753148.1
    CDS135..5615
    Translation

    Target ORF information:

    RefSeq Version XM_031897288.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897288.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACC TCCGATACCT
    GTTGACAGGC TTCTGATGGC TACTGGCAAG CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC
    AAGACTTCAG ACTCCGAAGT TAAAGCAAGA ATTGCTGCTA AAATAAGAAG AGCCCCTCCA
    TCTTTCGTCC CCCTTTCAGC TCTACAGCAT GAGGAGGGAT ACTGGCTGCT GGATGAAAAG
    CTCGGATCTC TATTGAACAT TAATGTCAAT TACTTGATAG ATGTGTTTTT ATCCAAGAAC
    GGGATTCAGT CTCTTGGGAG GAAAGGAAAA GAAGAGGTGC TGGCACTTAT TGCGACCCTC
    TTGGTGCTGC AAATGATTCG CACGTATAAG TTGGTGGACA TAACATTTAA GAGTCTGATG
    AAACTGGACG AATCATTTTG CACAAGCCCC TTTTACCAGA CAATTGAGAA ATCCGTCAAA
    TGGGCACGAA AAAAAGATGA ACAATATCCT GGCATCTGTT CTCGACTTGG ATTGGGCAAG
    GATTGGGAAT CTGCCACCCG GAAGTTATTA TCCATTGATC CAGTGGATCT TTCCTCTGAT
    CTCTATCCTG CTGTTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52874
    Clone ID Related Accession (Same CDS sequence) XM_031897289.1
    Accession Version XM_031897289.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAATGTG TCATATGAGT CCTCCCCGAT CAGAGGCACA ACCTTCTGAA
    CATGATGGAG AGCGAGAATG GCGGACGTTA CATGCCGAGC AGGAAGTGGA GCGCATAGGT
    GGAAGGCATG GTGGACCGCA TATCGATCGA GGGAACAGGA GAGGCGCATG GATGCCAGAG
    CGAACGGAGC CTGCGATGGT AGAAGCTGCC ATGCCAATGC GCCATATGGA TCGTTGCGGA
    ACAGAGGCAC AACCTTCTGA ACATGTGATG CAGGAAAGAG CTGCCAAGCC AATGTGTCTT
    ATGAGTCTTC CCCGATCAGT GATGCAGGAA AGAGCTGCCG AGGCAATGCG CCATTTGGGT
    TTTCGCCGAA CAGAAATCAT GGACATGCTG TCTCCAATGC CTCCATCCAA CGCTCCAATC
    TCTCCATCAT ATTGTGAAAA GCGCAAGGTT CACGCTGACA TGCTAGAAAT CATGGACATG
    CCGGCTCCAA CCTCTCCATC ATATGCTCCA ACCTCTCCAT CATATCTTCA AAGGCGCAAG
    AGCTTTTCTC TTATGGGCGC AGCTCCAAGC TCTGCTGTTT CGTATGCAGC TCCAACTTTT
    GGTTCTGCAA CTGCAGGGTT TACAAGCTCT GCTGTTTCGG ATGCAGCTCC AGCTTTTGGC
    TCTGCAGCTG CAAGACCTCC ATTATTTGGT TATAAAAGCT CTGCTGTTTC GGATGCAGCT
    CCAGGTTTCG GCACAGTGAC TGCTGCTCAG AGCTCTGGCT TTTTTTGTTC AGCAGGTTTC
    AGATTTGGAG CTCTAGTTCC ACCTCCACCT CCGATACCTG GCGGGGTTTG CAGATTTGCA
    CCGCCACCTC CACCTCCAGT TCCGGATCCA CTTCTGATGG CTACTGGCAG GGTTTGCAGA
    TTTGCACCGC CACCTCCACC TCCAGTTCCG GATCCACTTC TGATGGCTAC TGGCAGGCTT
    CGGATACCTG CTGGCGGGGT TTTAAGCTTT GCACCTCCAC CTCCACCTCC AGCTCCAGTT
    CCGGATCCAC CTCCGATACC TGTTGACAGG CTTCTGATGG CTACTGGCAA GCCTCGGAAA
    CATGCTGGCA GGGTCAGAGC CAAGGCTTTG TTCGCTCTAG CTCCTAGTGC TCCAACTTCA
    GACTCCGAAG TTAAAGCAAG AATTGCTGCT AAAATAAGAA GAGCCCCTCC ATCTTTCGTC
    CCCCTTTCAG CTCTACAGCA TGAGGAGGGA TACTGGCTGC TGGATGAAAA GCTCGGATCT
    CTATTGAACA TTAATGTCAA TTACTTGATA GATGTGTTTT TATCCAAGAA CGGGATTCAG
    TCTCTTGGGA GGAAAGGAAA AGAAGAGGTG CTGGCACTTA TTGCGACCCT CTTGGTGCTG
    CAAATGATTC GCACGTATAA GTTGGTGGAC ATAACATTTA AGAGTCTGAT GAAACTGGAC
    GAATCATTTT GCACAAGCCC CTTTTACCAG ACAATTGAGA AATCCGTCAA ATGGGCACGA
    AAAAAAGATG AACAATATCC TGGCATCTGT TCTCGACTTG GATTGGGCAA GGATTGGGAA
    TCTGCCACCC GGAAGTTATT ATCCATTGAT CCAGTGGATC TTTCCTCTGA TCTCTATCCT
    GCTGTTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753149.1
    CDS135..5606
    Translation

    Target ORF information:

    RefSeq Version XM_031897289.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897289.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAATGTG TCATATGAGT CCTCCCCGAT CAGAGGCACA ACCTTCTGAA
    CATGATGGAG AGCGAGAATG GCGGACGTTA CATGCCGAGC AGGAAGTGGA GCGCATAGGT
    GGAAGGCATG GTGGACCGCA TATCGATCGA GGGAACAGGA GAGGCGCATG GATGCCAGAG
    CGAACGGAGC CTGCGATGGT AGAAGCTGCC ATGCCAATGC GCCATATGGA TCGTTGCGGA
    ACAGAGGCAC AACCTTCTGA ACATGTGATG CAGGAAAGAG CTGCCAAGCC AATGTGTCTT
    ATGAGTCTTC CCCGATCAGT GATGCAGGAA AGAGCTGCCG AGGCAATGCG CCATTTGGGT
    TTTCGCCGAA CAGAAATCAT GGACATGCTG TCTCCAATGC CTCCATCCAA CGCTCCAATC
    TCTCCATCAT ATTGTGAAAA GCGCAAGGTT CACGCTGACA TGCTAGAAAT CATGGACATG
    CCGGCTCCAA CCTCTCCATC ATATGCTCCA ACCTCTCCAT CATATCTTCA AAGGCGCAAG
    AGCTTTTCTC TTATGGGCGC AGCTCCAAGC TCTGCTGTTT CGTATGCAGC TCCAACTTTT
    GGTTCTGCAA CTGCAGGGTT TACAAGCTCT GCTGTTTCGG ATGCAGCTCC AGCTTTTGGC
    TCTGCAGCTG CAAGACCTCC ATTATTTGGT TATAAAAGCT CTGCTGTTTC GGATGCAGCT
    CCAGGTTTCG GCACAGTGAC TGCTGCTCAG AGCTCTGGCT TTTTTTGTTC AGCAGGTTTC
    AGATTTGGAG CTCTAGTTCC ACCTCCACCT CCGATACCTG GCGGGGTTTG CAGATTTGCA
    CCGCCACCTC CACCTCCAGT TCCGGATCCA CTTCTGATGG CTACTGGCAG GGTTTGCAGA
    TTTGCACCGC CACCTCCACC TCCAGTTCCG GATCCACTTC TGATGGCTAC TGGCAGGCTT
    CGGATACCTG CTGGCGGGGT TTTAAGCTTT GCACCTCCAC CTCCACCTCC AGCTCCAGTT
    CCGGATCCAC CTCCGATACC TGTTGACAGG CTTCTGATGG CTACTGGCAA GCCTCGGAAA
    CATGCTGGCA GGGTCAGAGC CAAGGCTTTG TTCGCTCTAG CTCCTAGTGC TCCAACTTCA
    GACTCCGAAG TTAAAGCAAG AATTGCTGCT AAAATAAGAA GAGCCCCTCC ATCTTTCGTC
    CCCCTTTCAG CTCTACAGCA TGAGGAGGGA TACTGGCTGC TGGATGAAAA GCTCGGATCT
    CTATTGAACA TTAATGTCAA TTACTTGATA GATGTGTTTT TATCCAAGAA CGGGATTCAG
    TCTCTTGGGA GGAAAGGAAA AGAAGAGGTG CTGGCACTTA TTGCGACCCT CTTGGTGCTG
    CAAATGATTC GCACGTATAA GTTGGTGGAC ATAACATTTA AGAGTCTGAT GAAACTGGAC
    GAATCATTTT GCACAAGCCC CTTTTACCAG ACAATTGAGA AATCCGTCAA ATGGGCACGA
    AAAAAAGATG AACAATATCC TGGCATCTGT TCTCGACTTG GATTGGGCAA GGATTGGGAA
    TCTGCCACCC GGAAGTTATT ATCCATTGAT CCAGTGGATC TTTCCTCTGA TCTCTATCCT
    GCTGTTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52875
    Clone ID Related Accession (Same CDS sequence) XM_031897290.1
    Accession Version XM_031897290.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAG TTTGCAGATT TGCACCGCCA CCTCCACCTC CAGTTCCGGA TCCACTTCGG
    ATACCTGCTG GCGGGGTTTT AAGCTTTGCA CCTCCACCTC CACCTCCAGC TCCAGTTCCG
    GATCCACCTC CGATACCTGT TGACAGGCTT CTGATGGCTA CTGGCAAGCC TCGGAAACAT
    GCTGGCAGGG TCAGAGCCAA GGCTTTGTTC GCTCTAGCTC CTAGTGCTCC AACTTCAGAC
    TCCGAAGTTA AAGCAAGAAT TGCTGCTAAA ATAAGAAGAG CCCCTCCATC TTTCGTCCCC
    CTTTCAGCTC TACAGCATGA GGAGGGATAC TGGCTGCTGG ATGAAAAGCT CGGATCTCTA
    TTGAACATTA ATGTCAATTA CTTGATAGAT GTGTTTTTAT CCAAGAACGG GATTCAGTCT
    CTTGGGAGGA AAGGAAAAGA AGAGGTGCTG GCACTTATTG CGACCCTCTT GGTGCTGCAA
    ATGATTCGCA CGTATAAGTT GGTGGACATA ACATTTAAGA GTCTGATGAA ACTGGACGAA
    TCATTTTGCA CAAGCCCCTT TTACCAGACA ATTGAGAAAT CCGTCAAATG GGCACGAAAA
    AAAGATGAAC AATATCCTGG CATCTGTTCT CGACTTGGAT TGGGCAAGGA TTGGGAATCT
    GCCACCCGGA AGTTATTATC CATTGATCCA GTGGATCTTT CCTCTGATCT CTATCCTGCT
    GTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753150.1
    CDS135..5603
    Translation

    Target ORF information:

    RefSeq Version XM_031897290.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897290.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAG TTTGCAGATT TGCACCGCCA CCTCCACCTC CAGTTCCGGA TCCACTTCGG
    ATACCTGCTG GCGGGGTTTT AAGCTTTGCA CCTCCACCTC CACCTCCAGC TCCAGTTCCG
    GATCCACCTC CGATACCTGT TGACAGGCTT CTGATGGCTA CTGGCAAGCC TCGGAAACAT
    GCTGGCAGGG TCAGAGCCAA GGCTTTGTTC GCTCTAGCTC CTAGTGCTCC AACTTCAGAC
    TCCGAAGTTA AAGCAAGAAT TGCTGCTAAA ATAAGAAGAG CCCCTCCATC TTTCGTCCCC
    CTTTCAGCTC TACAGCATGA GGAGGGATAC TGGCTGCTGG ATGAAAAGCT CGGATCTCTA
    TTGAACATTA ATGTCAATTA CTTGATAGAT GTGTTTTTAT CCAAGAACGG GATTCAGTCT
    CTTGGGAGGA AAGGAAAAGA AGAGGTGCTG GCACTTATTG CGACCCTCTT GGTGCTGCAA
    ATGATTCGCA CGTATAAGTT GGTGGACATA ACATTTAAGA GTCTGATGAA ACTGGACGAA
    TCATTTTGCA CAAGCCCCTT TTACCAGACA ATTGAGAAAT CCGTCAAATG GGCACGAAAA
    AAAGATGAAC AATATCCTGG CATCTGTTCT CGACTTGGAT TGGGCAAGGA TTGGGAATCT
    GCCACCCGGA AGTTATTATC CATTGATCCA GTGGATCTTT CCTCTGATCT CTATCCTGCT
    GTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52876
    Clone ID Related Accession (Same CDS sequence) XM_031897291.1
    Accession Version XM_031897291.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCG GATACCTGCT GGCGGGGTTT TAAGCTTTGC ACCTCCACCT
    CCACCTCCAG CTCCAGTTCC GGATCCACTT CTGATGGCTA CTGGCAAGCC TCGGAAACAT
    GCTGGCAGGG TCAGAGCCAA GGCTTTGTTC GCTCTAGCTC CTAGTGCTCC AACTTCAGAC
    TCCGAAGTTA AAGCAAGAAT TGCTGCTAAA ATAAGAAGAG CCCCTCCATC TTTCGTCCCC
    CTTTCAGCTC TACAGCATGA GGAGGGATAC TGGCTGCTGG ATGAAAAGCT CGGATCTCTA
    TTGAACATTA ATGTCAATTA CTTGATAGAT GTGTTTTTAT CCAAGAACGG GATTCAGTCT
    CTTGGGAGGA AAGGAAAAGA AGAGGTGCTG GCACTTATTG CGACCCTCTT GGTGCTGCAA
    ATGATTCGCA CGTATAAGTT GGTGGACATA ACATTTAAGA GTCTGATGAA ACTGGACGAA
    TCATTTTGCA CAAGCCCCTT TTACCAGACA ATTGAGAAAT CCGTCAAATG GGCACGAAAA
    AAAGATGAAC AATATCCTGG CATCTGTTCT CGACTTGGAT TGGGCAAGGA TTGGGAATCT
    GCCACCCGGA AGTTATTATC CATTGATCCA GTGGATCTTT CCTCTGATCT CTATCCTGCT
    GTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753151.1
    CDS135..5603
    Translation

    Target ORF information:

    RefSeq Version XM_031897291.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897291.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCG GATACCTGCT GGCGGGGTTT TAAGCTTTGC ACCTCCACCT
    CCACCTCCAG CTCCAGTTCC GGATCCACTT CTGATGGCTA CTGGCAAGCC TCGGAAACAT
    GCTGGCAGGG TCAGAGCCAA GGCTTTGTTC GCTCTAGCTC CTAGTGCTCC AACTTCAGAC
    TCCGAAGTTA AAGCAAGAAT TGCTGCTAAA ATAAGAAGAG CCCCTCCATC TTTCGTCCCC
    CTTTCAGCTC TACAGCATGA GGAGGGATAC TGGCTGCTGG ATGAAAAGCT CGGATCTCTA
    TTGAACATTA ATGTCAATTA CTTGATAGAT GTGTTTTTAT CCAAGAACGG GATTCAGTCT
    CTTGGGAGGA AAGGAAAAGA AGAGGTGCTG GCACTTATTG CGACCCTCTT GGTGCTGCAA
    ATGATTCGCA CGTATAAGTT GGTGGACATA ACATTTAAGA GTCTGATGAA ACTGGACGAA
    TCATTTTGCA CAAGCCCCTT TTACCAGACA ATTGAGAAAT CCGTCAAATG GGCACGAAAA
    AAAGATGAAC AATATCCTGG CATCTGTTCT CGACTTGGAT TGGGCAAGGA TTGGGAATCT
    GCCACCCGGA AGTTATTATC CATTGATCCA GTGGATCTTT CCTCTGATCT CTATCCTGCT
    GTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52877
    Clone ID Related Accession (Same CDS sequence) XM_031897292.1
    Accession Version XM_031897292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5460bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACC TCCGATACCT
    GTTGACAGGC TTCTGATGGC TACTGGCAAG CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC
    AAGGCAAGAA TTGCTGCTAA AATAAGAAGA GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT
    CTACAGCATG AGGAGGGATA CTGGCTGCTG GATGAAAAGC TCGGATCTCT ATTGAACATT
    AATGTCAATT ACTTGATAGA TGTGTTTTTA TCCAAGAACG GGATTCAGTC TCTTGGGAGG
    AAAGGAAAAG AAGAGGTGCT GGCACTTATT GCGACCCTCT TGGTGCTGCA AATGATTCGC
    ACGTATAAGT TGGTGGACAT AACATTTAAG AGTCTGATGA AACTGGACGA ATCATTTTGC
    ACAAGCCCCT TTTACCAGAC AATTGAGAAA TCCGTCAAAT GGGCACGAAA AAAAGATGAA
    CAATATCCTG GCATCTGTTC TCGACTTGGA TTGGGCAAGG ATTGGGAATC TGCCACCCGG
    AAGTTATTAT CCATTGATCC AGTGGATCTT TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753152.1
    CDS135..5594
    Translation

    Target ORF information:

    RefSeq Version XM_031897292.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACC TCCGATACCT
    GTTGACAGGC TTCTGATGGC TACTGGCAAG CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC
    AAGGCAAGAA TTGCTGCTAA AATAAGAAGA GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT
    CTACAGCATG AGGAGGGATA CTGGCTGCTG GATGAAAAGC TCGGATCTCT ATTGAACATT
    AATGTCAATT ACTTGATAGA TGTGTTTTTA TCCAAGAACG GGATTCAGTC TCTTGGGAGG
    AAAGGAAAAG AAGAGGTGCT GGCACTTATT GCGACCCTCT TGGTGCTGCA AATGATTCGC
    ACGTATAAGT TGGTGGACAT AACATTTAAG AGTCTGATGA AACTGGACGA ATCATTTTGC
    ACAAGCCCCT TTTACCAGAC AATTGAGAAA TCCGTCAAAT GGGCACGAAA AAAAGATGAA
    CAATATCCTG GCATCTGTTC TCGACTTGGA TTGGGCAAGG ATTGGGAATC TGCCACCCGG
    AAGTTATTAT CCATTGATCC AGTGGATCTT TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52878
    Clone ID Related Accession (Same CDS sequence) XM_031897293.1
    Accession Version XM_031897293.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5460bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGACTTCAGA CTCCGAAGTT
    AAAGCAAGAA TTGCTGCTAA AATAAGAAGA GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT
    CTACAGCATG AGGAGGGATA CTGGCTGCTG GATGAAAAGC TCGGATCTCT ATTGAACATT
    AATGTCAATT ACTTGATAGA TGTGTTTTTA TCCAAGAACG GGATTCAGTC TCTTGGGAGG
    AAAGGAAAAG AAGAGGTGCT GGCACTTATT GCGACCCTCT TGGTGCTGCA AATGATTCGC
    ACGTATAAGT TGGTGGACAT AACATTTAAG AGTCTGATGA AACTGGACGA ATCATTTTGC
    ACAAGCCCCT TTTACCAGAC AATTGAGAAA TCCGTCAAAT GGGCACGAAA AAAAGATGAA
    CAATATCCTG GCATCTGTTC TCGACTTGGA TTGGGCAAGG ATTGGGAATC TGCCACCCGG
    AAGTTATTAT CCATTGATCC AGTGGATCTT TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753153.1
    CDS135..5594
    Translation

    Target ORF information:

    RefSeq Version XM_031897293.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGACTTCAGA CTCCGAAGTT
    AAAGCAAGAA TTGCTGCTAA AATAAGAAGA GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT
    CTACAGCATG AGGAGGGATA CTGGCTGCTG GATGAAAAGC TCGGATCTCT ATTGAACATT
    AATGTCAATT ACTTGATAGA TGTGTTTTTA TCCAAGAACG GGATTCAGTC TCTTGGGAGG
    AAAGGAAAAG AAGAGGTGCT GGCACTTATT GCGACCCTCT TGGTGCTGCA AATGATTCGC
    ACGTATAAGT TGGTGGACAT AACATTTAAG AGTCTGATGA AACTGGACGA ATCATTTTGC
    ACAAGCCCCT TTTACCAGAC AATTGAGAAA TCCGTCAAAT GGGCACGAAA AAAAGATGAA
    CAATATCCTG GCATCTGTTC TCGACTTGGA TTGGGCAAGG ATTGGGAATC TGCCACCCGG
    AAGTTATTAT CCATTGATCC AGTGGATCTT TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52879
    Clone ID Related Accession (Same CDS sequence) XM_031897294.1 , XM_031897295.1
    Accession Version XM_031897294.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG CTTCGGATAC CTGCTGGCGG GGTTTTAAGC
    TTTGCACCTC CACCTCCACC TCCAGCTCCA GTTCCGGATC CACCTCCGAT ACCTGTTGAC
    AGGCTTCTGA TGGCTACTGG CAAGCCTCGG AAACATGCTG GCAGGGTCAG AGCCAAGGCT
    TTGTTCGCTC TAGCTCCTAG TGCTCCAACT TCAGACTCCG AAGTTAAAGC AAGAATTGCT
    GCTAAAATAA GAAGAGCCCC TCCATCTTTC GTCCCCCTTT CAGCTCTACA GCATGAGGAG
    GGATACTGGC TGCTGGATGA AAAGCTCGGA TCTCTATTGA ACATTAATGT CAATTACTTG
    ATAGATGTGT TTTTATCCAA GAACGGGATT CAGTCTCTTG GGAGGAAAGG AAAAGAAGAG
    GTGCTGGCAC TTATTGCGAC CCTCTTGGTG CTGCAAATGA TTCGCACGTA TAAGTTGGTG
    GACATAACAT TTAAGAGTCT GATGAAACTG GACGAATCAT TTTGCACAAG CCCCTTTTAC
    CAGACAATTG AGAAATCCGT CAAATGGGCA CGAAAAAAAG ATGAACAATA TCCTGGCATC
    TGTTCTCGAC TTGGATTGGG CAAGGATTGG GAATCTGCCA CCCGGAAGTT ATTATCCATT
    GATCCAGTGG ATCTTTCCTC TGATCTCTAT CCTGCTGTTG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753154.1
    CDS135..5579
    Translation

    Target ORF information:

    RefSeq Version XM_031897294.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897294.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG CTTCGGATAC CTGCTGGCGG GGTTTTAAGC
    TTTGCACCTC CACCTCCACC TCCAGCTCCA GTTCCGGATC CACCTCCGAT ACCTGTTGAC
    AGGCTTCTGA TGGCTACTGG CAAGCCTCGG AAACATGCTG GCAGGGTCAG AGCCAAGGCT
    TTGTTCGCTC TAGCTCCTAG TGCTCCAACT TCAGACTCCG AAGTTAAAGC AAGAATTGCT
    GCTAAAATAA GAAGAGCCCC TCCATCTTTC GTCCCCCTTT CAGCTCTACA GCATGAGGAG
    GGATACTGGC TGCTGGATGA AAAGCTCGGA TCTCTATTGA ACATTAATGT CAATTACTTG
    ATAGATGTGT TTTTATCCAA GAACGGGATT CAGTCTCTTG GGAGGAAAGG AAAAGAAGAG
    GTGCTGGCAC TTATTGCGAC CCTCTTGGTG CTGCAAATGA TTCGCACGTA TAAGTTGGTG
    GACATAACAT TTAAGAGTCT GATGAAACTG GACGAATCAT TTTGCACAAG CCCCTTTTAC
    CAGACAATTG AGAAATCCGT CAAATGGGCA CGAAAAAAAG ATGAACAATA TCCTGGCATC
    TGTTCTCGAC TTGGATTGGG CAAGGATTGG GAATCTGCCA CCCGGAAGTT ATTATCCATT
    GATCCAGTGG ATCTTTCCTC TGATCTCTAT CCTGCTGTTG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52879
    Clone ID Related Accession (Same CDS sequence) XM_031897294.1 , XM_031897295.1
    Accession Version XM_031897295.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG CTTCGGATAC CTGCTGGCGG GGTTTTAAGC
    TTTGCACCTC CACCTCCACC TCCAGCTCCA GTTCCGGATC CACCTCCGAT ACCTGTTGAC
    AGGCTTCTGA TGGCTACTGG CAAGCCTCGG AAACATGCTG GCAGGGTCAG AGCCAAGGCT
    TTGTTCGCTC TAGCTCCTAG TGCTCCAACT TCAGACTCCG AAGTTAAAGC AAGAATTGCT
    GCTAAAATAA GAAGAGCCCC TCCATCTTTC GTCCCCCTTT CAGCTCTACA GCATGAGGAG
    GGATACTGGC TGCTGGATGA AAAGCTCGGA TCTCTATTGA ACATTAATGT CAATTACTTG
    ATAGATGTGT TTTTATCCAA GAACGGGATT CAGTCTCTTG GGAGGAAAGG AAAAGAAGAG
    GTGCTGGCAC TTATTGCGAC CCTCTTGGTG CTGCAAATGA TTCGCACGTA TAAGTTGGTG
    GACATAACAT TTAAGAGTCT GATGAAACTG GACGAATCAT TTTGCACAAG CCCCTTTTAC
    CAGACAATTG AGAAATCCGT CAAATGGGCA CGAAAAAAAG ATGAACAATA TCCTGGCATC
    TGTTCTCGAC TTGGATTGGG CAAGGATTGG GAATCTGCCA CCCGGAAGTT ATTATCCATT
    GATCCAGTGG ATCTTTCCTC TGATCTCTAT CCTGCTGTTG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753155.1
    CDS135..5579
    Translation

    Target ORF information:

    RefSeq Version XM_031897295.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897295.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG CTTCGGATAC CTGCTGGCGG GGTTTTAAGC
    TTTGCACCTC CACCTCCACC TCCAGCTCCA GTTCCGGATC CACCTCCGAT ACCTGTTGAC
    AGGCTTCTGA TGGCTACTGG CAAGCCTCGG AAACATGCTG GCAGGGTCAG AGCCAAGGCT
    TTGTTCGCTC TAGCTCCTAG TGCTCCAACT TCAGACTCCG AAGTTAAAGC AAGAATTGCT
    GCTAAAATAA GAAGAGCCCC TCCATCTTTC GTCCCCCTTT CAGCTCTACA GCATGAGGAG
    GGATACTGGC TGCTGGATGA AAAGCTCGGA TCTCTATTGA ACATTAATGT CAATTACTTG
    ATAGATGTGT TTTTATCCAA GAACGGGATT CAGTCTCTTG GGAGGAAAGG AAAAGAAGAG
    GTGCTGGCAC TTATTGCGAC CCTCTTGGTG CTGCAAATGA TTCGCACGTA TAAGTTGGTG
    GACATAACAT TTAAGAGTCT GATGAAACTG GACGAATCAT TTTGCACAAG CCCCTTTTAC
    CAGACAATTG AGAAATCCGT CAAATGGGCA CGAAAAAAAG ATGAACAATA TCCTGGCATC
    TGTTCTCGAC TTGGATTGGG CAAGGATTGG GAATCTGCCA CCCGGAAGTT ATTATCCATT
    GATCCAGTGG ATCTTTCCTC TGATCTCTAT CCTGCTGTTG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52880
    Clone ID Related Accession (Same CDS sequence) XM_031897296.1
    Accession Version XM_031897296.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCATGTTAT
    ATGGCTGACT TTGAATTGGC AAAAAGCAAG AAAAAAGCAA AACGTGGACC TATAGAAACA
    GAAGAGGCAC AACCTTTCAG ACGTATGAGA CGTGTAACTA CAATGTGTCA TATGAGTCCT
    CCCCGATCAG AGGCACAACC TTCTGAACAT GATGGAGAGC GAGAATGGCG GACGTTACAT
    GCCGAGCAGG AAGTGGAGCG CATAGGTGGA AGGCATGGTG GACCGCATAT CGATCGAGGG
    AACAGGAGAG GCGCATGGAT GCCAGAGCGA ACGGAGCCTG CGATGGTAGA AGCTGCCATG
    CCAATGCGCC ATATGGATCG TTGCGGAACA GAGGCACAAC CTTCTGAACA TGTGATGCAG
    GAAAGAGCTG CCAAGCCAAT GTGTCTTATG AGTCTTCCCC GATCAGTGAT GCAGGAAAGA
    GCTGCCGAGG CAATGCGCCA TTTGGGTTTT CGCCGAACAG AAATCATGGA CATGCTGTCT
    CCAATGCCTC CATCCAACGC TCCAATCTCT CCATCATATT GTGAAAAGCG CAAGGTTCAC
    GCTGACATGC TAGAAATCAT GGACATGCCG GCTCCAACCT CTCCATCATA TGCTCCAACC
    TCTCCATCAT ATCTTCAAAG GCGCAAGAGC TTTTCTCTTA TGGGCGCAGC TCCAAGCTCT
    GCTGTTTCGT ATGCAGCTCC AACTTTTGGT TCTGCAACTG CAGGGTTTAC AAGCTCTGCT
    GTTTCGGATG CAGCTCCAGC TTTTGGCTCT GCAGCTGCAA GACCTCCATT ATTTGGTTAT
    AAAAGCTCTG CTGTTTCGGA TGCAGCTCCA GGTTTCGGCA CAGTGACTGC TGCTCAGAGC
    TCTGGCTTTT TTTGTTCAGC AGGTTTCAGA TTTGGAGCTC TAGTTCCACC TCCACCTCCG
    ATACCTGGCG GGGTTTGCAG ATTTGCACCG CCACCTCCAC CTCCAGTTCC GGATCCACTT
    CTGATGGCTA CTGGCAGGGT TTGCAGATTT GCACCGCCAC CTCCACCTCC AGTTCCGGAT
    CCACTTCTGA TGGCTACTGG CAGGCTTCGG ATACCTGCTG GCGGGGTTTT AAGCTTTGCA
    CCTCCACCTC CACCTCCAGC TCCAGTTCCG GATCCACCTC CGATACCTGT TGACAGGCTT
    CTGATGGCTA CTGGCAAGCC TCGGAAACAT GCTGGCAGGG TCAGAGCCAA GGCTTTGTTC
    GCTCTAGCTC CTAGTGCTCC AACTTCAGAC TCCGAAGTTA AAGCAAGAAT TGCTGCTAAA
    ATAAGAAGAG CCCCTCCATC TTTCGTCCCC CTTTCAGCTC TACAGCATGA GGAGGGATAC
    TGGCTGCTGG ATGAAAAGCT CGGATCTCTA TTGAACATTA ATGTCAATTA CTTGATAGAT
    GTGTTTTTAT CCAAGAACGG GATTCAGTCT CTTGGGAGGA AAGGAAAAGA AGAGGTGCTG
    GCACTTATTG CGACCCTCTT GGTGCTGCAA ATGATTCGCA CGTATAAGTT GGTGGACATA
    ACATTTAAGA GTCTGATGAA ACTGGACGAA TCATTTTGCA CAAGCCCCTT TTACCAGACA
    ATTGAGAAAT CCGTCAAATG GGCACGAAAA AAAGATGAAC AATATCCTGG CATCTGTTCT
    CGACTTGGAT TGGGCAAGGA TTGGGAATCT GCCACCCGGA AGTTATTATC CATTGATCCA
    GTGGATCTTT CCTCTGATCT CTATCCTGCT GTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753156.1
    CDS135..5573
    Translation

    Target ORF information:

    RefSeq Version XM_031897296.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897296.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCATGTTAT
    ATGGCTGACT TTGAATTGGC AAAAAGCAAG AAAAAAGCAA AACGTGGACC TATAGAAACA
    GAAGAGGCAC AACCTTTCAG ACGTATGAGA CGTGTAACTA CAATGTGTCA TATGAGTCCT
    CCCCGATCAG AGGCACAACC TTCTGAACAT GATGGAGAGC GAGAATGGCG GACGTTACAT
    GCCGAGCAGG AAGTGGAGCG CATAGGTGGA AGGCATGGTG GACCGCATAT CGATCGAGGG
    AACAGGAGAG GCGCATGGAT GCCAGAGCGA ACGGAGCCTG CGATGGTAGA AGCTGCCATG
    CCAATGCGCC ATATGGATCG TTGCGGAACA GAGGCACAAC CTTCTGAACA TGTGATGCAG
    GAAAGAGCTG CCAAGCCAAT GTGTCTTATG AGTCTTCCCC GATCAGTGAT GCAGGAAAGA
    GCTGCCGAGG CAATGCGCCA TTTGGGTTTT CGCCGAACAG AAATCATGGA CATGCTGTCT
    CCAATGCCTC CATCCAACGC TCCAATCTCT CCATCATATT GTGAAAAGCG CAAGGTTCAC
    GCTGACATGC TAGAAATCAT GGACATGCCG GCTCCAACCT CTCCATCATA TGCTCCAACC
    TCTCCATCAT ATCTTCAAAG GCGCAAGAGC TTTTCTCTTA TGGGCGCAGC TCCAAGCTCT
    GCTGTTTCGT ATGCAGCTCC AACTTTTGGT TCTGCAACTG CAGGGTTTAC AAGCTCTGCT
    GTTTCGGATG CAGCTCCAGC TTTTGGCTCT GCAGCTGCAA GACCTCCATT ATTTGGTTAT
    AAAAGCTCTG CTGTTTCGGA TGCAGCTCCA GGTTTCGGCA CAGTGACTGC TGCTCAGAGC
    TCTGGCTTTT TTTGTTCAGC AGGTTTCAGA TTTGGAGCTC TAGTTCCACC TCCACCTCCG
    ATACCTGGCG GGGTTTGCAG ATTTGCACCG CCACCTCCAC CTCCAGTTCC GGATCCACTT
    CTGATGGCTA CTGGCAGGGT TTGCAGATTT GCACCGCCAC CTCCACCTCC AGTTCCGGAT
    CCACTTCTGA TGGCTACTGG CAGGCTTCGG ATACCTGCTG GCGGGGTTTT AAGCTTTGCA
    CCTCCACCTC CACCTCCAGC TCCAGTTCCG GATCCACCTC CGATACCTGT TGACAGGCTT
    CTGATGGCTA CTGGCAAGCC TCGGAAACAT GCTGGCAGGG TCAGAGCCAA GGCTTTGTTC
    GCTCTAGCTC CTAGTGCTCC AACTTCAGAC TCCGAAGTTA AAGCAAGAAT TGCTGCTAAA
    ATAAGAAGAG CCCCTCCATC TTTCGTCCCC CTTTCAGCTC TACAGCATGA GGAGGGATAC
    TGGCTGCTGG ATGAAAAGCT CGGATCTCTA TTGAACATTA ATGTCAATTA CTTGATAGAT
    GTGTTTTTAT CCAAGAACGG GATTCAGTCT CTTGGGAGGA AAGGAAAAGA AGAGGTGCTG
    GCACTTATTG CGACCCTCTT GGTGCTGCAA ATGATTCGCA CGTATAAGTT GGTGGACATA
    ACATTTAAGA GTCTGATGAA ACTGGACGAA TCATTTTGCA CAAGCCCCTT TTACCAGACA
    ATTGAGAAAT CCGTCAAATG GGCACGAAAA AAAGATGAAC AATATCCTGG CATCTGTTCT
    CGACTTGGAT TGGGCAAGGA TTGGGAATCT GCCACCCGGA AGTTATTATC CATTGATCCA
    GTGGATCTTT CCTCTGATCT CTATCCTGCT GTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52881
    Clone ID Related Accession (Same CDS sequence) XM_031897297.1
    Accession Version XM_031897297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCAAGAAT TGCTGCTAAA
    ATAAGAAGAG CCCCTCCATC TTTCGTCCCC CTTTCAGCTC TACAGCATGA GGAGGGATAC
    TGGCTGCTGG ATGAAAAGCT CGGATCTCTA TTGAACATTA ATGTCAATTA CTTGATAGAT
    GTGTTTTTAT CCAAGAACGG GATTCAGTCT CTTGGGAGGA AAGGAAAAGA AGAGGTGCTG
    GCACTTATTG CGACCCTCTT GGTGCTGCAA ATGATTCGCA CGTATAAGTT GGTGGACATA
    ACATTTAAGA GTCTGATGAA ACTGGACGAA TCATTTTGCA CAAGCCCCTT TTACCAGACA
    ATTGAGAAAT CCGTCAAATG GGCACGAAAA AAAGATGAAC AATATCCTGG CATCTGTTCT
    CGACTTGGAT TGGGCAAGGA TTGGGAATCT GCCACCCGGA AGTTATTATC CATTGATCCA
    GTGGATCTTT CCTCTGATCT CTATCCTGCT GTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753157.1
    CDS135..5573
    Translation

    Target ORF information:

    RefSeq Version XM_031897297.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACT TCTGATGGCT
    ACTGGCAAGC CTCGGAAACA TGCTGGCAGG GTCAGAGCCA AGGCAAGAAT TGCTGCTAAA
    ATAAGAAGAG CCCCTCCATC TTTCGTCCCC CTTTCAGCTC TACAGCATGA GGAGGGATAC
    TGGCTGCTGG ATGAAAAGCT CGGATCTCTA TTGAACATTA ATGTCAATTA CTTGATAGAT
    GTGTTTTTAT CCAAGAACGG GATTCAGTCT CTTGGGAGGA AAGGAAAAGA AGAGGTGCTG
    GCACTTATTG CGACCCTCTT GGTGCTGCAA ATGATTCGCA CGTATAAGTT GGTGGACATA
    ACATTTAAGA GTCTGATGAA ACTGGACGAA TCATTTTGCA CAAGCCCCTT TTACCAGACA
    ATTGAGAAAT CCGTCAAATG GGCACGAAAA AAAGATGAAC AATATCCTGG CATCTGTTCT
    CGACTTGGAT TGGGCAAGGA TTGGGAATCT GCCACCCGGA AGTTATTATC CATTGATCCA
    GTGGATCTTT CCTCTGATCT CTATCCTGCT GTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52882
    Clone ID Related Accession (Same CDS sequence) XM_031897298.1 , XM_031897299.1
    Accession Version XM_031897298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5424bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCGGATACC TGCTGGCGGG GTTTTAAGCT TTGCACCTCC ACCTCCACCT
    CCAGCTCCAG TTCCGGATCC ACCTCCGATA CCTGTTGACA GGCTTCTGAT GGCTACTGGC
    AAGCCTCGGA AACATGCTGG CAGGGTCAGA GCCAAGGCTT TGTTCGCTCT AGCTCCTAGT
    GCTCCAACTT CAGACTCCGA AGTTAAAGCA AGAATTGCTG CTAAAATAAG AAGAGCCCCT
    CCATCTTTCG TCCCCCTTTC AGCTCTACAG CATGAGGAGG GATACTGGCT GCTGGATGAA
    AAGCTCGGAT CTCTATTGAA CATTAATGTC AATTACTTGA TAGATGTGTT TTTATCCAAG
    AACGGGATTC AGTCTCTTGG GAGGAAAGGA AAAGAAGAGG TGCTGGCACT TATTGCGACC
    CTCTTGGTGC TGCAAATGAT TCGCACGTAT AAGTTGGTGG ACATAACATT TAAGAGTCTG
    ATGAAACTGG ACGAATCATT TTGCACAAGC CCCTTTTACC AGACAATTGA GAAATCCGTC
    AAATGGGCAC GAAAAAAAGA TGAACAATAT CCTGGCATCT GTTCTCGACT TGGATTGGGC
    AAGGATTGGG AATCTGCCAC CCGGAAGTTA TTATCCATTG ATCCAGTGGA TCTTTCCTCT
    GATCTCTATC CTGCTGTTGT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753158.1
    CDS135..5558
    Translation

    Target ORF information:

    RefSeq Version XM_031897298.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCGGATACC TGCTGGCGGG GTTTTAAGCT TTGCACCTCC ACCTCCACCT
    CCAGCTCCAG TTCCGGATCC ACCTCCGATA CCTGTTGACA GGCTTCTGAT GGCTACTGGC
    AAGCCTCGGA AACATGCTGG CAGGGTCAGA GCCAAGGCTT TGTTCGCTCT AGCTCCTAGT
    GCTCCAACTT CAGACTCCGA AGTTAAAGCA AGAATTGCTG CTAAAATAAG AAGAGCCCCT
    CCATCTTTCG TCCCCCTTTC AGCTCTACAG CATGAGGAGG GATACTGGCT GCTGGATGAA
    AAGCTCGGAT CTCTATTGAA CATTAATGTC AATTACTTGA TAGATGTGTT TTTATCCAAG
    AACGGGATTC AGTCTCTTGG GAGGAAAGGA AAAGAAGAGG TGCTGGCACT TATTGCGACC
    CTCTTGGTGC TGCAAATGAT TCGCACGTAT AAGTTGGTGG ACATAACATT TAAGAGTCTG
    ATGAAACTGG ACGAATCATT TTGCACAAGC CCCTTTTACC AGACAATTGA GAAATCCGTC
    AAATGGGCAC GAAAAAAAGA TGAACAATAT CCTGGCATCT GTTCTCGACT TGGATTGGGC
    AAGGATTGGG AATCTGCCAC CCGGAAGTTA TTATCCATTG ATCCAGTGGA TCTTTCCTCT
    GATCTCTATC CTGCTGTTGT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52882
    Clone ID Related Accession (Same CDS sequence) XM_031897298.1 , XM_031897299.1
    Accession Version XM_031897299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5424bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCGGATACC TGCTGGCGGG GTTTTAAGCT TTGCACCTCC ACCTCCACCT
    CCAGCTCCAG TTCCGGATCC ACCTCCGATA CCTGTTGACA GGCTTCTGAT GGCTACTGGC
    AAGCCTCGGA AACATGCTGG CAGGGTCAGA GCCAAGGCTT TGTTCGCTCT AGCTCCTAGT
    GCTCCAACTT CAGACTCCGA AGTTAAAGCA AGAATTGCTG CTAAAATAAG AAGAGCCCCT
    CCATCTTTCG TCCCCCTTTC AGCTCTACAG CATGAGGAGG GATACTGGCT GCTGGATGAA
    AAGCTCGGAT CTCTATTGAA CATTAATGTC AATTACTTGA TAGATGTGTT TTTATCCAAG
    AACGGGATTC AGTCTCTTGG GAGGAAAGGA AAAGAAGAGG TGCTGGCACT TATTGCGACC
    CTCTTGGTGC TGCAAATGAT TCGCACGTAT AAGTTGGTGG ACATAACATT TAAGAGTCTG
    ATGAAACTGG ACGAATCATT TTGCACAAGC CCCTTTTACC AGACAATTGA GAAATCCGTC
    AAATGGGCAC GAAAAAAAGA TGAACAATAT CCTGGCATCT GTTCTCGACT TGGATTGGGC
    AAGGATTGGG AATCTGCCAC CCGGAAGTTA TTATCCATTG ATCCAGTGGA TCTTTCCTCT
    GATCTCTATC CTGCTGTTGT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753159.1
    CDS135..5558
    Translation

    Target ORF information:

    RefSeq Version XM_031897299.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCGGATACC TGCTGGCGGG GTTTTAAGCT TTGCACCTCC ACCTCCACCT
    CCAGCTCCAG TTCCGGATCC ACCTCCGATA CCTGTTGACA GGCTTCTGAT GGCTACTGGC
    AAGCCTCGGA AACATGCTGG CAGGGTCAGA GCCAAGGCTT TGTTCGCTCT AGCTCCTAGT
    GCTCCAACTT CAGACTCCGA AGTTAAAGCA AGAATTGCTG CTAAAATAAG AAGAGCCCCT
    CCATCTTTCG TCCCCCTTTC AGCTCTACAG CATGAGGAGG GATACTGGCT GCTGGATGAA
    AAGCTCGGAT CTCTATTGAA CATTAATGTC AATTACTTGA TAGATGTGTT TTTATCCAAG
    AACGGGATTC AGTCTCTTGG GAGGAAAGGA AAAGAAGAGG TGCTGGCACT TATTGCGACC
    CTCTTGGTGC TGCAAATGAT TCGCACGTAT AAGTTGGTGG ACATAACATT TAAGAGTCTG
    ATGAAACTGG ACGAATCATT TTGCACAAGC CCCTTTTACC AGACAATTGA GAAATCCGTC
    AAATGGGCAC GAAAAAAAGA TGAACAATAT CCTGGCATCT GTTCTCGACT TGGATTGGGC
    AAGGATTGGG AATCTGCCAC CCGGAAGTTA TTATCCATTG ATCCAGTGGA TCTTTCCTCT
    GATCTCTATC CTGCTGTTGT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52883
    Clone ID Related Accession (Same CDS sequence) XM_031897300.1
    Accession Version XM_031897300.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA TGTTATATGG CTGACTTTGA ATTGGCAAAA
    AGCAAGAAAA AAGCAAAACG TGGACCTATA GAAACAGAAG AGGCACAACC TTTCAGACGT
    ATGAGACGTG TAACTACAAT GTGTCATATG AGTCCTCCCC GATCAGAGGC ACAACCTTCT
    GAACATGATG GAGAGCGAGA ATGGCGGACG TTACATGCCG AGCAGGAAGT GGAGCGCATA
    GGTGGAAGGC ATGGTGGACC GCATATCGAT CGAGGGAACA GGAGAGGCGC ATGGATGCCA
    GAGCGAACGG AGCCTGCGAT GGTAGAAGCT GCCATGCCAA TGCGCCATAT GGATCGTTGC
    GGAACAGAGG CACAACCTTC TGAACATGTG ATGCAGGAAA GAGCTGCCAA GCCAATGTGT
    CTTATGAGTC TTCCCCGATC AGTGATGCAG GAAAGAGCTG CCGAGGCAAT GCGCCATTTG
    GGTTTTCGCC GAACAGAAAT CATGGACATG CTGTCTCCAA TGCCTCCATC CAACGCTCCA
    ATCTCTCCAT CATATTGTGA AAAGCGCAAG GTTCACGCTG ACATGCTAGA AATCATGGAC
    ATGCCGGCTC CAACCTCTCC ATCATATGCT CCAACCTCTC CATCATATCT TCAAAGGCGC
    AAGAGCTTTT CTCTTATGGG CGCAGCTCCA AGCTCTGCTG TTTCGTATGC AGCTCCAACT
    TTTGGTTCTG CAACTGCAGG GTTTACAAGC TCTGCTGTTT CGGATGCAGC TCCAGCTTTT
    GGCTCTGCAG CTGCAAGACC TCCATTATTT GGTTATAAAA GCTCTGCTGT TTCGGATGCA
    GCTCCAGGTT TCGGCACAGT GACTGCTGCT CAGAGCTCTG GCTTTTTTTG TTCAGCAGGT
    TTCAGATTTG GAGCTCTAGT TCCACCTCCA CCTCCGATAC CTGGCGGGGT TTGCAGATTT
    GCACCGCCAC CTCCACCTCC AGTTCCGGAT CCACTTCTGA TGGCTACTGG CAGGGTTTGC
    AGATTTGCAC CGCCACCTCC ACCTCCAGTT CCGGATCCAC TTCTGATGGC TACTGGCAGG
    CTTCGGATAC CTGCTGGCGG GGTTTTAAGC TTTGCACCTC CACCTCCACC TCCAGCTCCA
    GTTCCGGATC CACCTCCGAT ACCTGTTGAC AGGCTTCTGA TGGCTACTGG CAAGCCTCGG
    AAACATGCTG GCAGGGTCAG AGCCAAGGCT TTGTTCGCTC TAGCTCCTAG TGCTCCAACT
    TCAGACTCCG AAGTTAAAGC AAGAATTGCT GCTAAAATAA GAAGAGCCCC TCCATCTTTC
    GTCCCCCTTT CAGCTCTACA GCATGAGGAG GGATACTGGC TGCTGGATGA AAAGCTCGGA
    TCTCTATTGA ACATTAATGT CAATTACTTG ATAGATGTGT TTTTATCCAA GAACGGGATT
    CAGTCTCTTG GGAGGAAAGG AAAAGAAGAG GTGCTGGCAC TTATTGCGAC CCTCTTGGTG
    CTGCAAATGA TTCGCACGTA TAAGTTGGTG GACATAACAT TTAAGAGTCT GATGAAACTG
    GACGAATCAT TTTGCACAAG CCCCTTTTAC CAGACAATTG AGAAATCCGT CAAATGGGCA
    CGAAAAAAAG ATGAACAATA TCCTGGCATC TGTTCTCGAC TTGGATTGGG CAAGGATTGG
    GAATCTGCCA CCCGGAAGTT ATTATCCATT GATCCAGTGG ATCTTTCCTC TGATCTCTAT
    CCTGCTGTTG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753160.1
    CDS135..5549
    Translation

    Target ORF information:

    RefSeq Version XM_031897300.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA TGTTATATGG CTGACTTTGA ATTGGCAAAA
    AGCAAGAAAA AAGCAAAACG TGGACCTATA GAAACAGAAG AGGCACAACC TTTCAGACGT
    ATGAGACGTG TAACTACAAT GTGTCATATG AGTCCTCCCC GATCAGAGGC ACAACCTTCT
    GAACATGATG GAGAGCGAGA ATGGCGGACG TTACATGCCG AGCAGGAAGT GGAGCGCATA
    GGTGGAAGGC ATGGTGGACC GCATATCGAT CGAGGGAACA GGAGAGGCGC ATGGATGCCA
    GAGCGAACGG AGCCTGCGAT GGTAGAAGCT GCCATGCCAA TGCGCCATAT GGATCGTTGC
    GGAACAGAGG CACAACCTTC TGAACATGTG ATGCAGGAAA GAGCTGCCAA GCCAATGTGT
    CTTATGAGTC TTCCCCGATC AGTGATGCAG GAAAGAGCTG CCGAGGCAAT GCGCCATTTG
    GGTTTTCGCC GAACAGAAAT CATGGACATG CTGTCTCCAA TGCCTCCATC CAACGCTCCA
    ATCTCTCCAT CATATTGTGA AAAGCGCAAG GTTCACGCTG ACATGCTAGA AATCATGGAC
    ATGCCGGCTC CAACCTCTCC ATCATATGCT CCAACCTCTC CATCATATCT TCAAAGGCGC
    AAGAGCTTTT CTCTTATGGG CGCAGCTCCA AGCTCTGCTG TTTCGTATGC AGCTCCAACT
    TTTGGTTCTG CAACTGCAGG GTTTACAAGC TCTGCTGTTT CGGATGCAGC TCCAGCTTTT
    GGCTCTGCAG CTGCAAGACC TCCATTATTT GGTTATAAAA GCTCTGCTGT TTCGGATGCA
    GCTCCAGGTT TCGGCACAGT GACTGCTGCT CAGAGCTCTG GCTTTTTTTG TTCAGCAGGT
    TTCAGATTTG GAGCTCTAGT TCCACCTCCA CCTCCGATAC CTGGCGGGGT TTGCAGATTT
    GCACCGCCAC CTCCACCTCC AGTTCCGGAT CCACTTCTGA TGGCTACTGG CAGGGTTTGC
    AGATTTGCAC CGCCACCTCC ACCTCCAGTT CCGGATCCAC TTCTGATGGC TACTGGCAGG
    CTTCGGATAC CTGCTGGCGG GGTTTTAAGC TTTGCACCTC CACCTCCACC TCCAGCTCCA
    GTTCCGGATC CACCTCCGAT ACCTGTTGAC AGGCTTCTGA TGGCTACTGG CAAGCCTCGG
    AAACATGCTG GCAGGGTCAG AGCCAAGGCT TTGTTCGCTC TAGCTCCTAG TGCTCCAACT
    TCAGACTCCG AAGTTAAAGC AAGAATTGCT GCTAAAATAA GAAGAGCCCC TCCATCTTTC
    GTCCCCCTTT CAGCTCTACA GCATGAGGAG GGATACTGGC TGCTGGATGA AAAGCTCGGA
    TCTCTATTGA ACATTAATGT CAATTACTTG ATAGATGTGT TTTTATCCAA GAACGGGATT
    CAGTCTCTTG GGAGGAAAGG AAAAGAAGAG GTGCTGGCAC TTATTGCGAC CCTCTTGGTG
    CTGCAAATGA TTCGCACGTA TAAGTTGGTG GACATAACAT TTAAGAGTCT GATGAAACTG
    GACGAATCAT TTTGCACAAG CCCCTTTTAC CAGACAATTG AGAAATCCGT CAAATGGGCA
    CGAAAAAAAG ATGAACAATA TCCTGGCATC TGTTCTCGAC TTGGATTGGG CAAGGATTGG
    GAATCTGCCA CCCGGAAGTT ATTATCCATT GATCCAGTGG ATCTTTCCTC TGATCTCTAT
    CCTGCTGTTG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52884
    Clone ID Related Accession (Same CDS sequence) XM_031897301.1
    Accession Version XM_031897301.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCGGATACC TGCTGGCGGG GTTTTAAGCT TTGCACCTCC ACCTCCACCT
    CCAGCTCCAG TTCCGGATCC ACCTCCGATA CCTGTTGACA GGCTTCTGAT GGCTACTGGC
    AAGCCTCGGA AACATGCTGG CAGGGTCAGA GCCAAGGCTT TGTTCGCTCT AGCTCCTAGT
    GCTCCAGCAA GAATTGCTGC TAAAATAAGA AGAGCCCCTC CATCTTTCGT CCCCCTTTCA
    GCTCTACAGC ATGAGGAGGG ATACTGGCTG CTGGATGAAA AGCTCGGATC TCTATTGAAC
    ATTAATGTCA ATTACTTGAT AGATGTGTTT TTATCCAAGA ACGGGATTCA GTCTCTTGGG
    AGGAAAGGAA AAGAAGAGGT GCTGGCACTT ATTGCGACCC TCTTGGTGCT GCAAATGATT
    CGCACGTATA AGTTGGTGGA CATAACATTT AAGAGTCTGA TGAAACTGGA CGAATCATTT
    TGCACAAGCC CCTTTTACCA GACAATTGAG AAATCCGTCA AATGGGCACG AAAAAAAGAT
    GAACAATATC CTGGCATCTG TTCTCGACTT GGATTGGGCA AGGATTGGGA ATCTGCCACC
    CGGAAGTTAT TATCCATTGA TCCAGTGGAT CTTTCCTCTG ATCTCTATCC TGCTGTTGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753161.1
    CDS135..5537
    Translation

    Target ORF information:

    RefSeq Version XM_031897301.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897301.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCGGATACC TGCTGGCGGG GTTTTAAGCT TTGCACCTCC ACCTCCACCT
    CCAGCTCCAG TTCCGGATCC ACCTCCGATA CCTGTTGACA GGCTTCTGAT GGCTACTGGC
    AAGCCTCGGA AACATGCTGG CAGGGTCAGA GCCAAGGCTT TGTTCGCTCT AGCTCCTAGT
    GCTCCAGCAA GAATTGCTGC TAAAATAAGA AGAGCCCCTC CATCTTTCGT CCCCCTTTCA
    GCTCTACAGC ATGAGGAGGG ATACTGGCTG CTGGATGAAA AGCTCGGATC TCTATTGAAC
    ATTAATGTCA ATTACTTGAT AGATGTGTTT TTATCCAAGA ACGGGATTCA GTCTCTTGGG
    AGGAAAGGAA AAGAAGAGGT GCTGGCACTT ATTGCGACCC TCTTGGTGCT GCAAATGATT
    CGCACGTATA AGTTGGTGGA CATAACATTT AAGAGTCTGA TGAAACTGGA CGAATCATTT
    TGCACAAGCC CCTTTTACCA GACAATTGAG AAATCCGTCA AATGGGCACG AAAAAAAGAT
    GAACAATATC CTGGCATCTG TTCTCGACTT GGATTGGGCA AGGATTGGGA ATCTGCCACC
    CGGAAGTTAT TATCCATTGA TCCAGTGGAT CTTTCCTCTG ATCTCTATCC TGCTGTTGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52885
    Clone ID Related Accession (Same CDS sequence) XM_031897302.1
    Accession Version XM_031897302.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCGGATACC TGCTGGCGGG GTTTTAAGCT TTGCACCTCC ACCTCCACCT
    CCAGCTCCAG TTCCGGATCC ACTTCTGATG GCTACTGGCA AGCCTCGGAA ACATGCTGGC
    AGGGTCAGAG CCAAGGCTTT GTTCGCTCTA GCTCCTAGTG CTCCAACTTC AGACTCCGAA
    GTTAAAGCAA GAATTGCTGC TAAAATAAGA AGAGCCCCTC CATCTTTCGT CCCCCTTTCA
    GCTCTACAGC ATGAGGAGGG ATACTGGCTG CTGGATGAAA AGCTCGGATC TCTATTGAAC
    ATTAATGTCA ATTACTTGAT AGATGTGTTT TTATCCAAGA ACGGGATTCA GTCTCTTGGG
    AGGAAAGGAA AAGAAGAGGT GCTGGCACTT ATTGCGACCC TCTTGGTGCT GCAAATGATT
    CGCACGTATA AGTTGGTGGA CATAACATTT AAGAGTCTGA TGAAACTGGA CGAATCATTT
    TGCACAAGCC CCTTTTACCA GACAATTGAG AAATCCGTCA AATGGGCACG AAAAAAAGAT
    GAACAATATC CTGGCATCTG TTCTCGACTT GGATTGGGCA AGGATTGGGA ATCTGCCACC
    CGGAAGTTAT TATCCATTGA TCCAGTGGAT CTTTCCTCTG ATCTCTATCC TGCTGTTGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753162.1
    CDS135..5537
    Translation

    Target ORF information:

    RefSeq Version XM_031897302.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897302.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCACCGGAT
    TTGTCTGGGT CTGATTTAGA CGATTATCGT CTGCCATTGT CTGGGTCTGA TTCAGATGAT
    TATTGTTGTT ATATGGCTGA CTTTGAATTG GCAAAAAGCA AGAAAAAAGC AAAACGTGGA
    CCTATAGAAA CAGAAGAGGC ACAACCTTTC AGACGTATGA GACGTGTAAC TACAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCGGATACC TGCTGGCGGG GTTTTAAGCT TTGCACCTCC ACCTCCACCT
    CCAGCTCCAG TTCCGGATCC ACTTCTGATG GCTACTGGCA AGCCTCGGAA ACATGCTGGC
    AGGGTCAGAG CCAAGGCTTT GTTCGCTCTA GCTCCTAGTG CTCCAACTTC AGACTCCGAA
    GTTAAAGCAA GAATTGCTGC TAAAATAAGA AGAGCCCCTC CATCTTTCGT CCCCCTTTCA
    GCTCTACAGC ATGAGGAGGG ATACTGGCTG CTGGATGAAA AGCTCGGATC TCTATTGAAC
    ATTAATGTCA ATTACTTGAT AGATGTGTTT TTATCCAAGA ACGGGATTCA GTCTCTTGGG
    AGGAAAGGAA AAGAAGAGGT GCTGGCACTT ATTGCGACCC TCTTGGTGCT GCAAATGATT
    CGCACGTATA AGTTGGTGGA CATAACATTT AAGAGTCTGA TGAAACTGGA CGAATCATTT
    TGCACAAGCC CCTTTTACCA GACAATTGAG AAATCCGTCA AATGGGCACG AAAAAAAGAT
    GAACAATATC CTGGCATCTG TTCTCGACTT GGATTGGGCA AGGATTGGGA ATCTGCCACC
    CGGAAGTTAT TATCCATTGA TCCAGTGGAT CTTTCCTCTG ATCTCTATCC TGCTGTTGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52886
    Clone ID Related Accession (Same CDS sequence) XM_031897303.1
    Accession Version XM_031897303.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCATGTTAT
    ATGGCTGACT TTGAATTGGC AAAAAGCAAG AAAAAAGCAA AACGTGGACC TATAGAAACA
    GAAATGTGTC ATATGAGTCC TCCCCGATCA GAGGCACAAC CTTCTGAACA TGATGGAGAG
    CGAGAATGGC GGACGTTACA TGCCGAGCAG GAAGTGGAGC GCATAGGTGG AAGGCATGGT
    GGACCGCATA TCGATCGAGG GAACAGGAGA GGCGCATGGA TGCCAGAGCG AACGGAGCCT
    GCGATGGTAG AAGCTGCCAT GCCAATGCGC CATATGGATC GTTGCGGAAC AGAGGCACAA
    CCTTCTGAAC ATGTGATGCA GGAAAGAGCT GCCAAGCCAA TGTGTCTTAT GAGTCTTCCC
    CGATCAGTGA TGCAGGAAAG AGCTGCCGAG GCAATGCGCC ATTTGGGTTT TCGCCGAACA
    GAAATCATGG ACATGCTGTC TCCAATGCCT CCATCCAACG CTCCAATCTC TCCATCATAT
    TGTGAAAAGC GCAAGGTTCA CGCTGACATG CTAGAAATCA TGGACATGCC GGCTCCAACC
    TCTCCATCAT ATGCTCCAAC CTCTCCATCA TATCTTCAAA GGCGCAAGAG CTTTTCTCTT
    ATGGGCGCAG CTCCAAGCTC TGCTGTTTCG TATGCAGCTC CAACTTTTGG TTCTGCAACT
    GCAGGGTTTA CAAGCTCTGC TGTTTCGGAT GCAGCTCCAG CTTTTGGCTC TGCAGCTGCA
    AGACCTCCAT TATTTGGTTA TAAAAGCTCT GCTGTTTCGG ATGCAGCTCC AGGTTTCGGC
    ACAGTGACTG CTGCTCAGAG CTCTGGCTTT TTTTGTTCAG CAGGTTTCAG ATTTGGAGCT
    CTAGTTCCAC CTCCACCTCC GATACCTGGC GGGGTTTGCA GATTTGCACC GCCACCTCCA
    CCTCCAGTTC CGGATCCACT TCTGATGGCT ACTGGCAGGG TTTGCAGATT TGCACCGCCA
    CCTCCACCTC CAGTTCCGGA TCCACTTCTG ATGGCTACTG GCAGGCTTCG GATACCTGCT
    GGCGGGGTTT TAAGCTTTGC ACCTCCACCT CCACCTCCAG CTCCAGTTCC GGATCCACCT
    CCGATACCTG TTGACAGGCT TCTGATGGCT ACTGGCAAGC CTCGGAAACA TGCTGGCAGG
    GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT CCTAGTGCTC CAACTTCAGA CTCCGAAGTT
    AAAGCAAGAA TTGCTGCTAA AATAAGAAGA GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT
    CTACAGCATG AGGAGGGATA CTGGCTGCTG GATGAAAAGC TCGGATCTCT ATTGAACATT
    AATGTCAATT ACTTGATAGA TGTGTTTTTA TCCAAGAACG GGATTCAGTC TCTTGGGAGG
    AAAGGAAAAG AAGAGGTGCT GGCACTTATT GCGACCCTCT TGGTGCTGCA AATGATTCGC
    ACGTATAAGT TGGTGGACAT AACATTTAAG AGTCTGATGA AACTGGACGA ATCATTTTGC
    ACAAGCCCCT TTTACCAGAC AATTGAGAAA TCCGTCAAAT GGGCACGAAA AAAAGATGAA
    CAATATCCTG GCATCTGTTC TCGACTTGGA TTGGGCAAGG ATTGGGAATC TGCCACCCGG
    AAGTTATTAT CCATTGATCC AGTGGATCTT TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753163.1
    CDS135..5534
    Translation

    Target ORF information:

    RefSeq Version XM_031897303.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897303.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCATGTTAT
    ATGGCTGACT TTGAATTGGC AAAAAGCAAG AAAAAAGCAA AACGTGGACC TATAGAAACA
    GAAATGTGTC ATATGAGTCC TCCCCGATCA GAGGCACAAC CTTCTGAACA TGATGGAGAG
    CGAGAATGGC GGACGTTACA TGCCGAGCAG GAAGTGGAGC GCATAGGTGG AAGGCATGGT
    GGACCGCATA TCGATCGAGG GAACAGGAGA GGCGCATGGA TGCCAGAGCG AACGGAGCCT
    GCGATGGTAG AAGCTGCCAT GCCAATGCGC CATATGGATC GTTGCGGAAC AGAGGCACAA
    CCTTCTGAAC ATGTGATGCA GGAAAGAGCT GCCAAGCCAA TGTGTCTTAT GAGTCTTCCC
    CGATCAGTGA TGCAGGAAAG AGCTGCCGAG GCAATGCGCC ATTTGGGTTT TCGCCGAACA
    GAAATCATGG ACATGCTGTC TCCAATGCCT CCATCCAACG CTCCAATCTC TCCATCATAT
    TGTGAAAAGC GCAAGGTTCA CGCTGACATG CTAGAAATCA TGGACATGCC GGCTCCAACC
    TCTCCATCAT ATGCTCCAAC CTCTCCATCA TATCTTCAAA GGCGCAAGAG CTTTTCTCTT
    ATGGGCGCAG CTCCAAGCTC TGCTGTTTCG TATGCAGCTC CAACTTTTGG TTCTGCAACT
    GCAGGGTTTA CAAGCTCTGC TGTTTCGGAT GCAGCTCCAG CTTTTGGCTC TGCAGCTGCA
    AGACCTCCAT TATTTGGTTA TAAAAGCTCT GCTGTTTCGG ATGCAGCTCC AGGTTTCGGC
    ACAGTGACTG CTGCTCAGAG CTCTGGCTTT TTTTGTTCAG CAGGTTTCAG ATTTGGAGCT
    CTAGTTCCAC CTCCACCTCC GATACCTGGC GGGGTTTGCA GATTTGCACC GCCACCTCCA
    CCTCCAGTTC CGGATCCACT TCTGATGGCT ACTGGCAGGG TTTGCAGATT TGCACCGCCA
    CCTCCACCTC CAGTTCCGGA TCCACTTCTG ATGGCTACTG GCAGGCTTCG GATACCTGCT
    GGCGGGGTTT TAAGCTTTGC ACCTCCACCT CCACCTCCAG CTCCAGTTCC GGATCCACCT
    CCGATACCTG TTGACAGGCT TCTGATGGCT ACTGGCAAGC CTCGGAAACA TGCTGGCAGG
    GTCAGAGCCA AGGCTTTGTT CGCTCTAGCT CCTAGTGCTC CAACTTCAGA CTCCGAAGTT
    AAAGCAAGAA TTGCTGCTAA AATAAGAAGA GCCCCTCCAT CTTTCGTCCC CCTTTCAGCT
    CTACAGCATG AGGAGGGATA CTGGCTGCTG GATGAAAAGC TCGGATCTCT ATTGAACATT
    AATGTCAATT ACTTGATAGA TGTGTTTTTA TCCAAGAACG GGATTCAGTC TCTTGGGAGG
    AAAGGAAAAG AAGAGGTGCT GGCACTTATT GCGACCCTCT TGGTGCTGCA AATGATTCGC
    ACGTATAAGT TGGTGGACAT AACATTTAAG AGTCTGATGA AACTGGACGA ATCATTTTGC
    ACAAGCCCCT TTTACCAGAC AATTGAGAAA TCCGTCAAAT GGGCACGAAA AAAAGATGAA
    CAATATCCTG GCATCTGTTC TCGACTTGGA TTGGGCAAGG ATTGGGAATC TGCCACCCGG
    AAGTTATTAT CCATTGATCC AGTGGATCTT TCCTCTGATC TCTATCCTGC TGTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52887
    Clone ID Related Accession (Same CDS sequence) XM_031897304.1
    Accession Version XM_031897304.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA TGTTATATGG CTGACTTTGA ATTGGCAAAA
    AGCAAGAAAA AAGCAAAACG TGGACCTATA GAAACAGAAA TGTGTCATAT GAGTCCTCCC
    CGATCAGAGG CACAACCTTC TGAACATGAT GGAGAGCGAG AATGGCGGAC GTTACATGCC
    GAGCAGGAAG TGGAGCGCAT AGGTGGAAGG CATGGTGGAC CGCATATCGA TCGAGGGAAC
    AGGAGAGGCG CATGGATGCC AGAGCGAACG GAGCCTGCGA TGGTAGAAGC TGCCATGCCA
    ATGCGCCATA TGGATCGTTG CGGAACAGAG GCACAACCTT CTGAACATGT GATGCAGGAA
    AGAGCTGCCA AGCCAATGTG TCTTATGAGT CTTCCCCGAT CAGTGATGCA GGAAAGAGCT
    GCCGAGGCAA TGCGCCATTT GGGTTTTCGC CGAACAGAAA TCATGGACAT GCTGTCTCCA
    ATGCCTCCAT CCAACGCTCC AATCTCTCCA TCATATTGTG AAAAGCGCAA GGTTCACGCT
    GACATGCTAG AAATCATGGA CATGCCGGCT CCAACCTCTC CATCATATGC TCCAACCTCT
    CCATCATATC TTCAAAGGCG CAAGAGCTTT TCTCTTATGG GCGCAGCTCC AAGCTCTGCT
    GTTTCGTATG CAGCTCCAAC TTTTGGTTCT GCAACTGCAG GGTTTACAAG CTCTGCTGTT
    TCGGATGCAG CTCCAGCTTT TGGCTCTGCA GCTGCAAGAC CTCCATTATT TGGTTATAAA
    AGCTCTGCTG TTTCGGATGC AGCTCCAGGT TTCGGCACAG TGACTGCTGC TCAGAGCTCT
    GGCTTTTTTT GTTCAGCAGG TTTCAGATTT GGAGCTCTAG TTCCACCTCC ACCTCCGATA
    CCTGGCGGGG TTTGCAGATT TGCACCGCCA CCTCCACCTC CAGTTCCGGA TCCACTTCTG
    ATGGCTACTG GCAGGGTTTG CAGATTTGCA CCGCCACCTC CACCTCCAGT TCCGGATCCA
    CTTCTGATGG CTACTGGCAG GCTTCGGATA CCTGCTGGCG GGGTTTTAAG CTTTGCACCT
    CCACCTCCAC CTCCAGCTCC AGTTCCGGAT CCACCTCCGA TACCTGTTGA CAGGCTTCTG
    ATGGCTACTG GCAAGCCTCG GAAACATGCT GGCAGGGTCA GAGCCAAGGC TTTGTTCGCT
    CTAGCTCCTA GTGCTCCAAC TTCAGACTCC GAAGTTAAAG CAAGAATTGC TGCTAAAATA
    AGAAGAGCCC CTCCATCTTT CGTCCCCCTT TCAGCTCTAC AGCATGAGGA GGGATACTGG
    CTGCTGGATG AAAAGCTCGG ATCTCTATTG AACATTAATG TCAATTACTT GATAGATGTG
    TTTTTATCCA AGAACGGGAT TCAGTCTCTT GGGAGGAAAG GAAAAGAAGA GGTGCTGGCA
    CTTATTGCGA CCCTCTTGGT GCTGCAAATG ATTCGCACGT ATAAGTTGGT GGACATAACA
    TTTAAGAGTC TGATGAAACT GGACGAATCA TTTTGCACAA GCCCCTTTTA CCAGACAATT
    GAGAAATCCG TCAAATGGGC ACGAAAAAAA GATGAACAAT ATCCTGGCAT CTGTTCTCGA
    CTTGGATTGG GCAAGGATTG GGAATCTGCC ACCCGGAAGT TATTATCCAT TGATCCAGTG
    GATCTTTCCT CTGATCTCTA TCCTGCTGTT GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753164.1
    CDS135..5510
    Translation

    Target ORF information:

    RefSeq Version XM_031897304.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897304.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA TGTTATATGG CTGACTTTGA ATTGGCAAAA
    AGCAAGAAAA AAGCAAAACG TGGACCTATA GAAACAGAAA TGTGTCATAT GAGTCCTCCC
    CGATCAGAGG CACAACCTTC TGAACATGAT GGAGAGCGAG AATGGCGGAC GTTACATGCC
    GAGCAGGAAG TGGAGCGCAT AGGTGGAAGG CATGGTGGAC CGCATATCGA TCGAGGGAAC
    AGGAGAGGCG CATGGATGCC AGAGCGAACG GAGCCTGCGA TGGTAGAAGC TGCCATGCCA
    ATGCGCCATA TGGATCGTTG CGGAACAGAG GCACAACCTT CTGAACATGT GATGCAGGAA
    AGAGCTGCCA AGCCAATGTG TCTTATGAGT CTTCCCCGAT CAGTGATGCA GGAAAGAGCT
    GCCGAGGCAA TGCGCCATTT GGGTTTTCGC CGAACAGAAA TCATGGACAT GCTGTCTCCA
    ATGCCTCCAT CCAACGCTCC AATCTCTCCA TCATATTGTG AAAAGCGCAA GGTTCACGCT
    GACATGCTAG AAATCATGGA CATGCCGGCT CCAACCTCTC CATCATATGC TCCAACCTCT
    CCATCATATC TTCAAAGGCG CAAGAGCTTT TCTCTTATGG GCGCAGCTCC AAGCTCTGCT
    GTTTCGTATG CAGCTCCAAC TTTTGGTTCT GCAACTGCAG GGTTTACAAG CTCTGCTGTT
    TCGGATGCAG CTCCAGCTTT TGGCTCTGCA GCTGCAAGAC CTCCATTATT TGGTTATAAA
    AGCTCTGCTG TTTCGGATGC AGCTCCAGGT TTCGGCACAG TGACTGCTGC TCAGAGCTCT
    GGCTTTTTTT GTTCAGCAGG TTTCAGATTT GGAGCTCTAG TTCCACCTCC ACCTCCGATA
    CCTGGCGGGG TTTGCAGATT TGCACCGCCA CCTCCACCTC CAGTTCCGGA TCCACTTCTG
    ATGGCTACTG GCAGGGTTTG CAGATTTGCA CCGCCACCTC CACCTCCAGT TCCGGATCCA
    CTTCTGATGG CTACTGGCAG GCTTCGGATA CCTGCTGGCG GGGTTTTAAG CTTTGCACCT
    CCACCTCCAC CTCCAGCTCC AGTTCCGGAT CCACCTCCGA TACCTGTTGA CAGGCTTCTG
    ATGGCTACTG GCAAGCCTCG GAAACATGCT GGCAGGGTCA GAGCCAAGGC TTTGTTCGCT
    CTAGCTCCTA GTGCTCCAAC TTCAGACTCC GAAGTTAAAG CAAGAATTGC TGCTAAAATA
    AGAAGAGCCC CTCCATCTTT CGTCCCCCTT TCAGCTCTAC AGCATGAGGA GGGATACTGG
    CTGCTGGATG AAAAGCTCGG ATCTCTATTG AACATTAATG TCAATTACTT GATAGATGTG
    TTTTTATCCA AGAACGGGAT TCAGTCTCTT GGGAGGAAAG GAAAAGAAGA GGTGCTGGCA
    CTTATTGCGA CCCTCTTGGT GCTGCAAATG ATTCGCACGT ATAAGTTGGT GGACATAACA
    TTTAAGAGTC TGATGAAACT GGACGAATCA TTTTGCACAA GCCCCTTTTA CCAGACAATT
    GAGAAATCCG TCAAATGGGC ACGAAAAAAA GATGAACAAT ATCCTGGCAT CTGTTCTCGA
    CTTGGATTGG GCAAGGATTG GGAATCTGCC ACCCGGAAGT TATTATCCAT TGATCCAGTG
    GATCTTTCCT CTGATCTCTA TCCTGCTGTT GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52888
    Clone ID Related Accession (Same CDS sequence) XM_031897305.1
    Accession Version XM_031897305.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACC TCCGATACCT
    GTTGACAGGC TTCTGATGGC TACTGGCAAG CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC
    AAGGCTTTGT TCGCTCTAGC TCCTAGTGCT CCAACTTCAG ACTCCGAAGT TAAAGCAAGA
    ATTGCTGCTA AAATAAGAAG AGCCCCTCCA TCTTTCGTCC CCCTTTCAGC TCTACAGCAT
    GAGGAGGGAT ACTGGCTGCT GGATGAAAAG CTCGGATCTC TATTGAACAT TAATGTCAAT
    TACTTGATAG ATGTGTTTTT ATCCAAGAAC GGGATTCAGT CTCTTGGGAG GAAAGGAAAA
    GAAGAGGTGC TGGCACTTAT TGCGACCCTC TTGGTGCTGC AAATGATTCG CACGTATAAG
    TTGGTGGACA TAACATTTAA GAGTCTGATG AAACTGGACG AATCATTTTG CACAAGCCCC
    TTTTACCAGA CAATTGAGAA ATCCGTCAAA TGGGCACGAA AAAAAGATGA ACAATATCCT
    GGCATCTGTT CTCGACTTGG ATTGGGCAAG GATTGGGAAT CTGCCACCCG GAAGTTATTA
    TCCATTGATC CAGTGGATCT TTCCTCTGAT CTCTATCCTG CTGTTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753165.1
    CDS135..5465
    Translation

    Target ORF information:

    RefSeq Version XM_031897305.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897305.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA GAAGAATATA TGGATCAAGA AGCAATGTGT
    CATATGAGTC CTCCCCGATC AGAGGCACAA CCTTCTGAAC ATGATGGAGA GCGAGAATGG
    CGGACGTTAC ATGCCGAGCA GGAAGTGGAG CGCATAGGTG GAAGGCATGG TGGACCGCAT
    ATCGATCGAG GGAACAGGAG AGGCGCATGG ATGCCAGAGC GAACGGAGCC TGCGATGGTA
    GAAGCTGCCA TGCCAATGCG CCATATGGAT CGTTGCGGAA CAGAGGCACA ACCTTCTGAA
    CATGTGATGC AGGAAAGAGC TGCCAAGCCA ATGTGTCTTA TGAGTCTTCC CCGATCAGTG
    ATGCAGGAAA GAGCTGCCGA GGCAATGCGC CATTTGGGTT TTCGCCGAAC AGAAATCATG
    GACATGCTGT CTCCAATGCC TCCATCCAAC GCTCCAATCT CTCCATCATA TTGTGAAAAG
    CGCAAGGTTC ACGCTGACAT GCTAGAAATC ATGGACATGC CGGCTCCAAC CTCTCCATCA
    TATGCTCCAA CCTCTCCATC ATATCTTCAA AGGCGCAAGA GCTTTTCTCT TATGGGCGCA
    GCTCCAAGCT CTGCTGTTTC GTATGCAGCT CCAACTTTTG GTTCTGCAAC TGCAGGGTTT
    ACAAGCTCTG CTGTTTCGGA TGCAGCTCCA GCTTTTGGCT CTGCAGCTGC AAGACCTCCA
    TTATTTGGTT ATAAAAGCTC TGCTGTTTCG GATGCAGCTC CAGGTTTCGG CACAGTGACT
    GCTGCTCAGA GCTCTGGCTT TTTTTGTTCA GCAGGTTTCA GATTTGGAGC TCTAGTTCCA
    CCTCCACCTC CGATACCTGG CGGGGTTTGC AGATTTGCAC CGCCACCTCC ACCTCCAGTT
    CCGGATCCAC TTCTGATGGC TACTGGCAGG GTTTGCAGAT TTGCACCGCC ACCTCCACCT
    CCAGTTCCGG ATCCACTTCT GATGGCTACT GGCAGGCTTC GGATACCTGC TGGCGGGGTT
    TTAAGCTTTG CACCTCCACC TCCACCTCCA GCTCCAGTTC CGGATCCACC TCCGATACCT
    GTTGACAGGC TTCTGATGGC TACTGGCAAG CCTCGGAAAC ATGCTGGCAG GGTCAGAGCC
    AAGGCTTTGT TCGCTCTAGC TCCTAGTGCT CCAACTTCAG ACTCCGAAGT TAAAGCAAGA
    ATTGCTGCTA AAATAAGAAG AGCCCCTCCA TCTTTCGTCC CCCTTTCAGC TCTACAGCAT
    GAGGAGGGAT ACTGGCTGCT GGATGAAAAG CTCGGATCTC TATTGAACAT TAATGTCAAT
    TACTTGATAG ATGTGTTTTT ATCCAAGAAC GGGATTCAGT CTCTTGGGAG GAAAGGAAAA
    GAAGAGGTGC TGGCACTTAT TGCGACCCTC TTGGTGCTGC AAATGATTCG CACGTATAAG
    TTGGTGGACA TAACATTTAA GAGTCTGATG AAACTGGACG AATCATTTTG CACAAGCCCC
    TTTTACCAGA CAATTGAGAA ATCCGTCAAA TGGGCACGAA AAAAAGATGA ACAATATCCT
    GGCATCTGTT CTCGACTTGG ATTGGGCAAG GATTGGGAAT CTGCCACCCG GAAGTTATTA
    TCCATTGATC CAGTGGATCT TTCCTCTGAT CTCTATCCTG CTGTTGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52889
    Clone ID Related Accession (Same CDS sequence) XM_031897306.1
    Accession Version XM_031897306.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein mono-ADP-ribosyltransferase PARP4 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA ATGTGTCATA TGAGTCCTCC CCGATCAGAG
    GCACAACCTT CTGAACATGA TGGAGAGCGA GAATGGCGGA CGTTACATGC CGAGCAGGAA
    GTGGAGCGCA TAGGTGGAAG GCATGGTGGA CCGCATATCG ATCGAGGGAA CAGGAGAGGC
    GCATGGATGC CAGAGCGAAC GGAGCCTGCG ATGGTAGAAG CTGCCATGCC AATGCGCCAT
    ATGGATCGTT GCGGAACAGA GGCACAACCT TCTGAACATG TGATGCAGGA AAGAGCTGCC
    AAGCCAATGT GTCTTATGAG TCTTCCCCGA TCAGTGATGC AGGAAAGAGC TGCCGAGGCA
    ATGCGCCATT TGGGTTTTCG CCGAACAGAA ATCATGGACA TGCTGTCTCC AATGCCTCCA
    TCCAACGCTC CAATCTCTCC ATCATATTGT GAAAAGCGCA AGGTTCACGC TGACATGCTA
    GAAATCATGG ACATGCCGGC TCCAACCTCT CCATCATATG CTCCAACCTC TCCATCATAT
    CTTCAAAGGC GCAAGAGCTT TTCTCTTATG GGCGCAGCTC CAAGCTCTGC TGTTTCGTAT
    GCAGCTCCAA CTTTTGGTTC TGCAACTGCA GGGTTTACAA GCTCTGCTGT TTCGGATGCA
    GCTCCAGCTT TTGGCTCTGC AGCTGCAAGA CCTCCATTAT TTGGTTATAA AAGCTCTGCT
    GTTTCGGATG CAGCTCCAGG TTTCGGCACA GTGACTGCTG CTCAGAGCTC TGGCTTTTTT
    TGTTCAGCAG GTTTCAGATT TGGAGCTCTA GTTCCACCTC CACCTCCGAT ACCTGGCGGG
    GTTTGCAGAT TTGCACCGCC ACCTCCACCT CCAGTTCCGG ATCCACTTCT GATGGCTACT
    GGCAGGGTTT GCAGATTTGC ACCGCCACCT CCACCTCCAG TTCCGGATCC ACTTCTGATG
    GCTACTGGCA GGCTTCGGAT ACCTGCTGGC GGGGTTTTAA GCTTTGCACC TCCACCTCCA
    CCTCCAGCTC CAGTTCCGGA TCCACCTCCG ATACCTGTTG ACAGGCTTCT GATGGCTACT
    GGCAAGCCTC GGAAACATGC TGGCAGGGTC AGAGCCAAGG CTTTGTTCGC TCTAGCTCCT
    AGTGCTCCAA CTTCAGACTC CGAAGTTAAA GCAAGAATTG CTGCTAAAAT AAGAAGAGCC
    CCTCCATCTT TCGTCCCCCT TTCAGCTCTA CAGCATGAGG AGGGATACTG GCTGCTGGAT
    GAAAAGCTCG GATCTCTATT GAACATTAAT GTCAATTACT TGATAGATGT GTTTTTATCC
    AAGAACGGGA TTCAGTCTCT TGGGAGGAAA GGAAAAGAAG AGGTGCTGGC ACTTATTGCG
    ACCCTCTTGG TGCTGCAAAT GATTCGCACG TATAAGTTGG TGGACATAAC ATTTAAGAGT
    CTGATGAAAC TGGACGAATC ATTTTGCACA AGCCCCTTTT ACCAGACAAT TGAGAAATCC
    GTCAAATGGG CACGAAAAAA AGATGAACAA TATCCTGGCA TCTGTTCTCG ACTTGGATTG
    GGCAAGGATT GGGAATCTGC CACCCGGAAG TTATTATCCA TTGATCCAGT GGATCTTTCC
    TCTGATCTCT ATCCTGCTGT TGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753166.1
    CDS135..5441
    Translation

    Target ORF information:

    RefSeq Version XM_031897306.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis protein mono-ADP-ribosyltransferase PARP4 (LOC100170623), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897306.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACTGTGG CGATCTTTTC AGACTGTGTG TTCTTCCTGA AACTAAACGA CTTATCTTTT 
    AATGAGAGAA AAGCGTTAAA ATTGACAGTA ACCTCGAATG GTGGAGTTAT ATCCTTTGTG
    CTAAATCAGA AGTGCACACA TGTTGTGGTG AACACCATTG GCTGTTTAAG TTCTTCTCAG
    CAGAAGAATG TTCAGAAGCA TCAGATTCCA ATTGTGGACA GTCAGTTTAT ATGGCAATGT
    CTAGAAAAGA AGTGTCTTAT CCAGGACTAT CAATATCCGA CTGCTGGTGT CAAACCAAGT
    GAGAAAGAAC CAGAAGGCTG GCAAGCAGAT CCTGAGAAAA CTGTCTTCCC TAAAAAAGTT
    CCATCCCTGC AAGACAATGA TGAGTTCATC AACAGGGATT ACAGTGAATA TGATGCAGAC
    ACCCTCCCCG ACCCTCCAGA TGCCGAAGTA GCAAAATACT GTTTTTTTCA AAATGGTCCC
    ATTGTGGCAG TTCTGGAGCT GTTGTGCATT CCTGAATCCT CTTCATTCCT ATTTCAAATA
    TCTAAAACAA TTTGTAATTC CAATGGATCG GAGCCTGACA GCTCCTATAT GTTCAAACAC
    ACAGCGGAGG CGGCTTGTAA CAAGTATGAG AAGTGCACTG AGGATTTAGA AAAAGAAGGC
    TTTAAGAGAG CAAAGCGTAT CCCACCAAAG GTTGCCCTGC ACACATCTGA GGCTCTGCAG
    AAGGTCCTGC TTGAAGAAGC CATAAATGCT ACCAAGTTAT CTCCAGATGT TGGATATTTT
    GTGGAAAGTG TTTGGATTGA GGCTCTTGGC CATTTGGATA ACATCCTCGC GCGTCCCGTA
    AACAGCATCA GTCTGAATGA TGTAAGCAAA GCAGAGGGTG TTCTGCTCCA GGTTAGGAAA
    GCTTTGGACA CAGGAACAGA ACCTGGAGAC ATAAGGAAAG CAATGGCTGA GTTTTATCGG
    CTCATTCCCC ATAAGAAACC TCTGCTGGCC AACATTGATA AGAAACTTTT GTCAACAAAG
    CAAGATCTGT GCCAGCTTAT TAGAGATATG GTGAGTGTAT GTGAAACCAA CTCATCGAAG
    CCAAACCCAA GCAGCCTTGC CAAATACCAG GCCCTGCGAT GCCAAATAGA GCATGTGGAG
    CCCAATACTG AGGAATTTCA TCAGGTGTCA CAGCAAATCG TGGAGAACAA ACACTGTGAG
    GAGTCGATTA ACATACTGAA AATATTCCGC GTTGGGAAAT TAAGTGAAGC CACTGGGTTT
    CAAAAGAATC TTGGGAATGT CAAGTCCCTG CTCCATGCAT CTTCCCCACG TCACTTTGTG
    GGGATCCTTT CCCGTGGACT GCTCTTGCCA AAAAATGTGG AAGATGATTT GGGTTTAAAA
    AGAACTGATA TTGGAAATCT TGGAAGTGGA ATTTACTTCA GTGATTCCAT AAGTACAAGT
    GTTAAATATT CGGAACCCAG TGTAACCAAT GGGGCGCGTC TCCTGTTGGT TTGTGAGGTG
    GCTCTGGGGA ACTGCAAGGA TCTCTACAAA AGAGACTTCT CCCTTACTGG AGCTCCAAGT
    GGCTTTAACA GCGTTCATGG TGTCCGACAA ATGCCAGGGA GGAAATCTGA TTTTGAGGAT
    GATGAATTTG TGGTATATAA AAAGGATCAA ATAAAAATGA GATACGCCGT GCAGTTTTGC
    ACTGATAAAG ACCAAGTCAA CATGGAACGC GACAAACTTT CTGTTCAGTT TGAGCCCATG
    GAGGAAGATG TTGGAGAAGA ACCAGTGCAG GTGGAAGATC TCATGTTGGA GAATCTTCCG
    GAACCACAGA GCATAAATGG CGGCCTCCAG AGTGTTCATG GCAACCAGAT CCCTTTGGAA
    AATATTCATG TGAAAGGCAA AATGATGGAT TTAGTTGCAG AGGTTATATT ATTCCAGACC
    TACATAAACA AATCCTCTGT TCCCATCGAG GCTAAATATG TTTTTCCCCT GGACGGCACA
    GCAGCCGTGT GTGGCTTTGA AGCATTTATA AATGGAAAAC ACGTTATCGG AGAGGTTAAA
    GAGAAGGAGC AAGCGCATAA GGAGTACAGG GCTGCTATCA GCGAGGGACA TGGGGCTTAC
    CTTATGGACC AGGACGCTGC TGACGTCTTC ACTGTAAGTG TTGGAAACCT GCCCCCAAAC
    GCCACTGTAA TCATTAAAAT CACGTACATA ACGGAATTGA TTCTGGAGTA TGGATCAGTG
    ATATTCAACA TTCCTGGCAC AGTGGCATCT TGGCAAAAGG ATAAAGCTCT GAAAGAAAAT
    ACTCAGGATA CAGTAGAAAA AGTCTGTGTT GAAGAAAGTG GTGTGCCACA AGGTTGTTTC
    TCTCTGGAGA TGTCTGTTGA GATGTCTTTT AAAATCGAGA ATGTGTTAAG TTTCACTCAC
    AGTATTTCAA CGAAAAAAAC TGATTGCAAA GCTGTGATTG CAACTAAAAA AGACAGTTCA
    TTGGATGACG AGGGGTTTCA GTTGAGAGTC TACCTGGAAT CAAGCTTCTT GGAGTCTTCT
    TGGAACCAAC CGAGAATGTG GGTGGAAAAA CACCCCAAAG AAGACAGTGA GGCATGCATG
    CTTGTGTTCC TGCCTAGTTT TAAGACAAGC ATACAATCCT GGGATTTAAC CATCTTACTT
    GATTGCTCCA ATTCTATGGA ATCCACTTTC CAAAGCGCAA AACGGATTGC TCTGCTTGCA
    GCCAGCTCGT TAAACCCATG GCACAACATA AACATAATCA GCTTTGGTAC AGGTCATAAG
    GAATTTAGCA TCCGTCCAAA AGAGAGCCAG AATTTAATAC CGGAGCTGGA GCAGTTTATT
    AAAATGGCCA AACCAAACAT GGGAAACACA GAACTTTGGA AGCCTCTGCA GTCCTTATGT
    TTGCTGGCTC CTCCCAGTGA CATGCACAAT GTTCTTCTTA TATCTGATGG ACACATCCAG
    AATGAGAGCC TCGTCTTTCA GATCCTCAAA AAGAATGCAG GGAAAGTGCG GCTGTTCACC
    TGTGGCGTTG GTGCAACAGC CAATCGCCAT ATGTTGCGGT GTTTGGCTCA GTACGGTGCT
    GGGTTCTTTG AATTTTTTGA AGATAAATCC AAGTCCAGCT GGAAAAAAAA GATGGAGGCA
    CAGCTGGAAA GAATGGATTC CCCTGCATGC ACTAGTGCAT CTGTTAAATG GATGCAGTTC
    AGTGATAATG ACCCAGAGCC CGTTCAAGCC CCAGCACACA TACCGGCTTT GTTCAACCGC
    AGTTTCCTTC TTGTTTATGG CTTTGCTCCT CGTTGCACTC AGGCCAAGCT ACAAGCTTTA
    ATTGATGACA ATGAACTGGA CACTATGGTA TCAACTACAG AATTACAGAA GACCAGAGGA
    ACGATGCTTC ATAAACTGAC GGCAAGGGCT GTTATTAAGG ATTATGAAGA TGGGATCCTG
    CACGAGAAAG AACATGAACA TGAGATGAAA AAGCAGCAAA TGAAGTCCTT TATTATTGAG
    CTGAGCAAGA AGTACTCAAT CGTCACTCAG TTTACAAGCT TTGTCGCTGT AGAGAAACGG
    GATGCACAAG AGAAGCCAAA GGACTATGTA CCAAATGTGC TTGAAATAAT ATCCACAGAA
    GATGTTGATA TATTACCATA CATGATGTGG GAAACACAGG AAGAGGATCA GGCCGCTGTG
    CTGCAGGAAG AAGATATGCA GGAAGAAGAA ATGTGTCATA TGAGTCCTCC CCGATCAGAG
    GCACAACCTT CTGAACATGA TGGAGAGCGA GAATGGCGGA CGTTACATGC CGAGCAGGAA
    GTGGAGCGCA TAGGTGGAAG GCATGGTGGA CCGCATATCG ATCGAGGGAA CAGGAGAGGC
    GCATGGATGC CAGAGCGAAC GGAGCCTGCG ATGGTAGAAG CTGCCATGCC AATGCGCCAT
    ATGGATCGTT GCGGAACAGA GGCACAACCT TCTGAACATG TGATGCAGGA AAGAGCTGCC
    AAGCCAATGT GTCTTATGAG TCTTCCCCGA TCAGTGATGC AGGAAAGAGC TGCCGAGGCA
    ATGCGCCATT TGGGTTTTCG CCGAACAGAA ATCATGGACA TGCTGTCTCC AATGCCTCCA
    TCCAACGCTC CAATCTCTCC ATCATATTGT GAAAAGCGCA AGGTTCACGC TGACATGCTA
    GAAATCATGG ACATGCCGGC TCCAACCTCT CCATCATATG CTCCAACCTC TCCATCATAT
    CTTCAAAGGC GCAAGAGCTT TTCTCTTATG GGCGCAGCTC CAAGCTCTGC TGTTTCGTAT
    GCAGCTCCAA CTTTTGGTTC TGCAACTGCA GGGTTTACAA GCTCTGCTGT TTCGGATGCA
    GCTCCAGCTT TTGGCTCTGC AGCTGCAAGA CCTCCATTAT TTGGTTATAA AAGCTCTGCT
    GTTTCGGATG CAGCTCCAGG TTTCGGCACA GTGACTGCTG CTCAGAGCTC TGGCTTTTTT
    TGTTCAGCAG GTTTCAGATT TGGAGCTCTA GTTCCACCTC CACCTCCGAT ACCTGGCGGG
    GTTTGCAGAT TTGCACCGCC ACCTCCACCT CCAGTTCCGG ATCCACTTCT GATGGCTACT
    GGCAGGGTTT GCAGATTTGC ACCGCCACCT CCACCTCCAG TTCCGGATCC ACTTCTGATG
    GCTACTGGCA GGCTTCGGAT ACCTGCTGGC GGGGTTTTAA GCTTTGCACC TCCACCTCCA
    CCTCCAGCTC CAGTTCCGGA TCCACCTCCG ATACCTGTTG ACAGGCTTCT GATGGCTACT
    GGCAAGCCTC GGAAACATGC TGGCAGGGTC AGAGCCAAGG CTTTGTTCGC TCTAGCTCCT
    AGTGCTCCAA CTTCAGACTC CGAAGTTAAA GCAAGAATTG CTGCTAAAAT AAGAAGAGCC
    CCTCCATCTT TCGTCCCCCT TTCAGCTCTA CAGCATGAGG AGGGATACTG GCTGCTGGAT
    GAAAAGCTCG GATCTCTATT GAACATTAAT GTCAATTACT TGATAGATGT GTTTTTATCC
    AAGAACGGGA TTCAGTCTCT TGGGAGGAAA GGAAAAGAAG AGGTGCTGGC ACTTATTGCG
    ACCCTCTTGG TGCTGCAAAT GATTCGCACG TATAAGTTGG TGGACATAAC ATTTAAGAGT
    CTGATGAAAC TGGACGAATC ATTTTGCACA AGCCCCTTTT ACCAGACAAT TGAGAAATCC
    GTCAAATGGG CACGAAAAAA AGATGAACAA TATCCTGGCA TCTGTTCTCG ACTTGGATTG
    GGCAAGGATT GGGAATCTGC CACCCGGAAG TTATTATCCA TTGATCCAGT GGATCTTTCC
    TCTGATCTCT ATCCTGCTGT TGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.