phldb1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	phldb1
Entrez Gene ID	100170555
Full Name	pleckstrin homology-like domain, family B, member 1
General protein information	Preferred Names pleckstrin homology-like domain, family B, member 1 Names pleckstrin homology-like domain family B member 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012966177.2	XP_012821631.1	pleckstrin homology-like domain family B member 1 isoform X13
XM_012966178.2	XP_012821632.1	pleckstrin homology-like domain family B member 1 isoform X14
XM_012966179.2	XP_012821633.1	pleckstrin homology-like domain family B member 1 isoform X15
XM_012966170.3	XP_012821624.1	pleckstrin homology-like domain family B member 1 isoform X6
XM_012966172.3	XP_012821626.1	pleckstrin homology-like domain family B member 1 isoform X9
XM_012966173.3	XP_012821627.1	pleckstrin homology-like domain family B member 1 isoform X10
XM_031904873.1	XP_031760733.1	pleckstrin homology-like domain family B member 1 isoform X16
XM_012966178.3	XP_012821632.1	pleckstrin homology-like domain family B member 1 isoform X17
XM_012966174.2	XP_012821628.1	pleckstrin homology-like domain family B member 1 isoform X20
XM_012966188.3	XP_012821642.1	pleckstrin homology-like domain family B member 1 isoform X25
XM_012966167.2	XP_012821621.1	pleckstrin homology-like domain family B member 1 isoform X2
XM_012966168.2	XP_012821622.1	pleckstrin homology-like domain family B member 1 isoform X3
XM_012966174.1	XP_012821628.1	pleckstrin homology-like domain family B member 1 isoform X9
XM_012966175.2	XP_012821629.1	pleckstrin homology-like domain family B member 1 isoform X10
XM_012966180.2	XP_012821634.1	pleckstrin homology-like domain family B member 1 isoform X16
XM_012966183.2	XP_012821637.1	pleckstrin homology-like domain family B member 1 isoform X18
XM_012966185.2	XP_012821639.1	pleckstrin homology-like domain family B member 1 isoform X20
XM_012966165.3	XP_012821619.1	pleckstrin homology-like domain family B member 1 isoform X1
XM_012966167.3	XP_012821621.1	pleckstrin homology-like domain family B member 1 isoform X2
XM_012966168.3	XP_012821622.1	pleckstrin homology-like domain family B member 1 isoform X4
XM_031904872.1	XP_031760732.1	pleckstrin homology-like domain family B member 1 isoform X14
XM_012966165.2	XP_012821619.1	pleckstrin homology-like domain family B member 1 isoform X1
XM_012966172.2	XP_012821626.1	pleckstrin homology-like domain family B member 1 isoform X7
XM_012966184.2	XP_012821638.1	pleckstrin homology-like domain family B member 1 isoform X19
NM_001130332.1	NP_001123804.1	pleckstrin homology-like domain family B member 1
XM_012966169.3	XP_012821623.1	pleckstrin homology-like domain family B member 1 isoform X5
XM_031904870.1	XP_031760730.1	pleckstrin homology-like domain family B member 1 isoform X8
XM_012966177.3	XP_012821631.1	pleckstrin homology-like domain family B member 1 isoform X15
XM_012966182.3	XP_012821636.1	pleckstrin homology-like domain family B member 1 isoform X21
XM_012966183.3	XP_012821637.1	pleckstrin homology-like domain family B member 1 isoform X22
XM_012966169.2	XP_012821623.1	pleckstrin homology-like domain family B member 1 isoform X4
XM_012966170.2	XP_012821624.1	pleckstrin homology-like domain family B member 1 isoform X5
XM_012966176.2	XP_012821630.1	pleckstrin homology-like domain family B member 1 isoform X11
XM_012966186.2	XP_012821640.1	pleckstrin homology-like domain family B member 1 isoform X21
XM_031904869.1	XP_031760729.1	pleckstrin homology-like domain family B member 1 isoform X3
XM_012966176.3	XP_012821630.1	pleckstrin homology-like domain family B member 1 isoform X12
XM_012966180.3	XP_012821634.1	pleckstrin homology-like domain family B member 1 isoform X19
XM_012966171.2	XP_012821625.1	pleckstrin homology-like domain family B member 1 isoform X6
XM_012966173.2	XP_012821627.1	pleckstrin homology-like domain family B member 1 isoform X8
XM_018095290.1	XP_017950779.1	pleckstrin homology-like domain family B member 1 isoform X12
XM_012966182.2	XP_012821636.1	pleckstrin homology-like domain family B member 1 isoform X17
XM_012966188.2	XP_012821642.1	pleckstrin homology-like domain family B member 1 isoform X22
XM_012966171.3	XP_012821625.1	pleckstrin homology-like domain family B member 1 isoform X7
XM_012966175.3	XP_012821629.1	pleckstrin homology-like domain family B member 1 isoform X11
XM_031904871.1	XP_031760731.1	pleckstrin homology-like domain family B member 1 isoform X13
XM_012966179.3	XP_012821633.1	pleckstrin homology-like domain family B member 1 isoform X18
XM_012966184.3	XP_012821638.1	pleckstrin homology-like domain family B member 1 isoform X23
XM_012966185.3	XP_012821639.1	pleckstrin homology-like domain family B member 1 isoform X24

Pathways from BioSystems

Homologies

Homo sapiens (human)	PHLDB1	NP_001138231.1
Gallus gallus (chicken)	PHLDB1	XP_417927.4
Danio rerio (zebrafish)	LOC570275	XP_005157508.1
Xenopus tropicalis (tropical clawed frog)	phldb1	NP_001123804.1
Canis lupus familiaris (dog)	PHLDB1	XP_005619762.1
Bos taurus (cattle)	PHLDB1	NP_001192971.1
Rattus norvegicus (Norway rat)	Phldb1	NP_001178507.1
Pan troglodytes (chimpanzee)	PHLDB1	XP_001162204.1
Mus musculus (house mouse)	Phldb1	NP_705765.3
	LOC706336	XP_002808134.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following phldb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phldb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa32409	XM_012966171.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32410	XM_012966172.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32411	XM_012966173.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32412	XM_012966174.2 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32413	XM_012966175.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32414	XM_012966176.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32415	XM_012966177.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32416	XM_012966178.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32417	XM_012966179.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32418	XM_012966180.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32419	XM_012966182.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32420	XM_012966183.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa32421	XM_012966184.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa32422	XM_012966185.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa32424	XM_012966188.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa45339	XM_018095290.1 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa61273	XM_031904870.1 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61274	XM_031904871.1 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61275	XM_031904872.1 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61276	XM_031904873.1 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32404	XM_012966165.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa32405	XM_012966167.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa32406	XM_012966168.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa32407	XM_012966169.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa32408	XM_012966170.3 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa61272	XM_031904869.1 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa02934	NM_001130332.1 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32423	XM_012966186.2 Latest version!	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OXa32416	XM_012966178.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32412	XM_012966174.1	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32405	XM_012966167.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa32406	XM_012966168.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa32404	XM_012966165.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa32410	XM_012966172.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32415	XM_012966177.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32420	XM_012966183.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa32407	XM_012966169.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa32408	XM_012966170.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa32414	XM_012966176.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32418	XM_012966180.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32411	XM_012966173.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32419	XM_012966182.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32424	XM_012966188.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32409	XM_012966171.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32413	XM_012966175.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32417	XM_012966179.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32421	XM_012966184.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa32422	XM_012966185.2	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phldb1 ( XM_012966171.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821625.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X7, mRNA.

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CloneID	OXa32409
Clone ID Related Accession (Same CDS sequence)	XM_012966171.2 , XM_012966171.3
Accession Version	XM_012966171.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966171.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821625.1
CDS	35..4525
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966171.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966171.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966172.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821626.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X9, mRNA.

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CloneID	OXa32410
Clone ID Related Accession (Same CDS sequence)	XM_012966172.3 , XM_012966172.2
Accession Version	XM_012966172.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966172.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGTACA TCACTTTAGA TCAGCTGCAG GATATTCTGG AAAGCATGCC CACAGGTTCT 
GCTCCAACTG CTGTGGCAAT GGCTGGCACT GATATCAACT CTGCCTCTTG TGCTCCTTCA 
CCTGACGACC CTCCTCCTGT ACATCTCTCG TCTTCATCTT CTTCCTCTCC CTCCATTTCT 
CTGGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT 
GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT 
CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG 
ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT 
CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG 
AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG 
AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA 
GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT 
AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821626.1
CDS	35..4492
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966172.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966172.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGTACA TCACTTTAGA TCAGCTGCAG GATATTCTGG AAAGCATGCC CACAGGTTCT 
GCTCCAACTG CTGTGGCAAT GGCTGGCACT GATATCAACT CTGCCTCTTG TGCTCCTTCA 
CCTGACGACC CTCCTCCTGT ACATCTCTCG TCTTCATCTT CTTCCTCTCC CTCCATTTCT 
CTGGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT 
GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT 
CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG 
ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT 
CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG 
AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG 
AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA 
GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT 
AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966173.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821627.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X10, mRNA.

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CloneID	OXa32411
Clone ID Related Accession (Same CDS sequence)	XM_012966173.3 , XM_012966173.2
Accession Version	XM_012966173.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966173.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAG TCCTAACCCA 
GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA 
GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC 
ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821627.1
CDS	35..4483
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966173.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966173.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAG TCCTAACCCA 
GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA 
GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC 
ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966174.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821628.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X20, mRNA.

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CloneID	OXa32412
Clone ID Related Accession (Same CDS sequence)	XM_012966174.2 , XM_012966174.1
Accession Version	XM_012966174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC TGAGTCATGA TGTGAGCAGC CCCTGCAGAA AAACACACAC AGTTATTCAG 
AGCACACCAT TGGATCTCAT AGATACAGGA AAAAGTCTTA GGGTGCAGAC AGAAAAACCT 
CATCTTGTCA GCCTGGGAAG TGGACGACTC AGCACTGCAA TCACCCTACT ACCACTGGAA 
GAAGGAAGGA CAACCCTGGG TCATGCATCT AAGGACATTC CCCTGCAGGG ACCTGGCATT 
GCTGCAGAAC ATTGCTACAT TGAAAACATC AGAGGAAACC TCACCCTATA CCCATGTGGA 
AATCCTTGTG CCATCGATGG ACTGCTCATC ACCCAACCGA CACGTCTCAC TCAAGGGTGT 
ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT 
TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC 
CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT 
TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC 
CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC 
TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT 
AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA 
ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT 
GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA 
ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT 
CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG 
AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG 
CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA 
GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT 
GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA 
TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG 
CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC 
AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG 
CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA 
ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC 
CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC 
TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT 
AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT 
ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA 
CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA 
GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC 
CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC 
CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG 
GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC 
GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG 
CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821628.1
CDS	195..4625
Translation	MKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966174.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966174.2

ORF Insert Sequence:

ATGAAGGCAC TGAGTCATGA TGTGAGCAGC CCCTGCAGAA AAACACACAC AGTTATTCAG 
AGCACACCAT TGGATCTCAT AGATACAGGA AAAAGTCTTA GGGTGCAGAC AGAAAAACCT 
CATCTTGTCA GCCTGGGAAG TGGACGACTC AGCACTGCAA TCACCCTACT ACCACTGGAA 
GAAGGAAGGA CAACCCTGGG TCATGCATCT AAGGACATTC CCCTGCAGGG ACCTGGCATT 
GCTGCAGAAC ATTGCTACAT TGAAAACATC AGAGGAAACC TCACCCTATA CCCATGTGGA 
AATCCTTGTG CCATCGATGG ACTGCTCATC ACCCAACCGA CACGTCTCAC TCAAGGGTGT 
ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT 
TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC 
CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT 
TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC 
CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC 
TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT 
AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA 
ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT 
GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA 
ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT 
CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG 
AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG 
CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA 
GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT 
GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA 
TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG 
CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC 
AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG 
CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA 
ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC 
CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC 
TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT 
AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT 
ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA 
CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA 
GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC 
CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC 
CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG 
GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC 
GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG 
CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966175.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821629.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OXa32413
Clone ID Related Accession (Same CDS sequence)	XM_012966175.2 , XM_012966175.3
Accession Version	XM_012966175.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821629.1
CDS	35..4465
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966175.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966175.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966176.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821630.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X12, mRNA.

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CloneID	OXa32414
Clone ID Related Accession (Same CDS sequence)	XM_012966176.2 , XM_012966176.3
Accession Version	XM_012966176.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGAT TTCTCGCTCT 
CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT 
TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT 
CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC 
AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT 
GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG 
AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG 
TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA 
GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT 
AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA 
GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC 
AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG 
AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA 
CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA 
CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC 
CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG 
ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG 
AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT 
TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT 
TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821630.1
CDS	35..4435
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966176.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966176.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGAT TTCTCGCTCT 
CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT 
TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT 
CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC 
AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT 
GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG 
AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG 
TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA 
GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT 
AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA 
GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC 
AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG 
AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA 
CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA 
CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC 
CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG 
ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG 
AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT 
TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT 
TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966177.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821631.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X15, mRNA.

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CloneID	OXa32415
Clone ID Related Accession (Same CDS sequence)	XM_012966177.2 , XM_012966177.3
Accession Version	XM_012966177.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGAT TTCTCGCTCT 
CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT 
TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT 
CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC 
AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT 
GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG 
AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG 
TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA 
GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT 
AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA 
GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC 
AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG 
AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA 
CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA 
CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC 
CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG 
ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG 
AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT 
TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT 
TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821631.1
CDS	35..4255
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966177.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966177.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGAT TTCTCGCTCT 
CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT 
TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT 
CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC 
AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT 
GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG 
AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG 
TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA 
GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT 
AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA 
GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC 
AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG 
AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA 
CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA 
CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC 
CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG 
ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG 
AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT 
TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT 
TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966178.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821632.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X17, mRNA.

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CloneID	OXa32416
Clone ID Related Accession (Same CDS sequence)	XM_012966178.2 , XM_012966178.3
Accession Version	XM_012966178.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966178.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA 
GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT 
CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT 
GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG 
CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT 
TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG 
TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA 
GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA 
AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG 
ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA 
CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC 
CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG 
ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG 
TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG 
CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT 
GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT 
GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT 
CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT 
GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821632.1
CDS	35..4153
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966178.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966178.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA 
GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT 
CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT 
GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG 
CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT 
TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG 
TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA 
GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA 
AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG 
ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA 
CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC 
CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG 
ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG 
TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG 
CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT 
GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT 
GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT 
CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT 
GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966179.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821633.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OXa32417
Clone ID Related Accession (Same CDS sequence)	XM_012966179.2 , XM_012966179.3
Accession Version	XM_012966179.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966179.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821633.1
CDS	35..4111
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966179.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966179.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966180.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821634.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X19, mRNA.

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CloneID	OXa32418
Clone ID Related Accession (Same CDS sequence)	XM_012966180.2 , XM_012966180.3
Accession Version	XM_012966180.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966180.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT 
TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC 
CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT 
TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC 
CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC 
TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT 
AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA 
ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT 
GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA 
ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT 
CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG 
AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG 
CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA 
GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT 
GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA 
TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG 
CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC 
AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG 
CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA 
ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC 
CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC 
TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT 
AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT 
ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA 
CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA 
GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC 
CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC 
CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG 
GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC 
GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG 
CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821634.1
CDS	622..4692
Translation	MVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966180.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966180.3

ORF Insert Sequence:

ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT 
TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC 
CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT 
TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC 
CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC 
TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT 
AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA 
ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT 
GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA 
ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT 
CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG 
AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG 
CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA 
GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT 
GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA 
TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG 
CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC 
AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG 
CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA 
ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC 
CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC 
TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT 
AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT 
ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA 
CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA 
GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC 
CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC 
CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG 
GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC 
GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG 
CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966182.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821636.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X21, mRNA.

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CloneID	OXa32419
Clone ID Related Accession (Same CDS sequence)	XM_012966182.3 , XM_012966182.2
Accession Version	XM_012966182.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA 
CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT 
GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG 
GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGACAGT 
CTGAGCACAG AAGATTCTCT TCCATCTGAA GTTGAAGGTC AACAAGTGAT AGAGGAGCAG 
CGCAGACGTC TTGCAGAGTT GAAACAGAGA GCTGCAGTTG AGGCACAATC TCAGTGGGAA 
TCTCTCCATG GAGCCCCTAA TCAAGGGTAC ACGCCTATAA TTCACCACTC CATTCTACAC 
CATCAGCGGC CTCTCCATTC TCGTGAAGAT GACCCTACCC ATGGATTTGA CACTCTCAGT 
CTGGAGAGTT CTGATAGTTT GGACACTAGT GTTTCTACAG GAAACTCTGC ATGTTCCCCT 
GATAACATGT CAAGTGCTAG TGGGTTAGAC ATGCTAAAGA TAGAGGAAAT GGAAAGAATG 
CTTCTAGAAG CTCATGCAGA GCGTGCCAGG CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG 
GCACGCAGAA AAGCACTAGA AGATGAGAGG CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT 
CAAGATGAGA CAGCTCACAG GCAAAGACTA GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG 
CATTTTTCAC AGGCTCGTCC TCTTACACGC TATCTGCCAA TCCGGAAGGA AGACTTTGAT 
CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT GCAGTAGATG CCTGCCCTCA CATCATTTTA 
AGTGAGAAGA TGTGTCGTGG CTTCCTGACA AAAATGGGTG GAAAGATAAA ATCATGGAAG 
AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA CGGACCCTGT CCTATTATGT AGATAAACAT 
GAGGCAAAGC TGAAAGGAGT TATATATTTC CAGGCCATAG AAGAGGTTTA TTATGACCAC 
CTCAGGAGTG CAGCCAAGAA AGGACTTTTT AACCTCAGCC TGGCCACTCA GATATCCGAT 
TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG 
ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG 
GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821636.1
CDS	35..4042
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966182.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966182.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA 
CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT 
GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG 
GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGACAGT 
CTGAGCACAG AAGATTCTCT TCCATCTGAA GTTGAAGGTC AACAAGTGAT AGAGGAGCAG 
CGCAGACGTC TTGCAGAGTT GAAACAGAGA GCTGCAGTTG AGGCACAATC TCAGTGGGAA 
TCTCTCCATG GAGCCCCTAA TCAAGGGTAC ACGCCTATAA TTCACCACTC CATTCTACAC 
CATCAGCGGC CTCTCCATTC TCGTGAAGAT GACCCTACCC ATGGATTTGA CACTCTCAGT 
CTGGAGAGTT CTGATAGTTT GGACACTAGT GTTTCTACAG GAAACTCTGC ATGTTCCCCT 
GATAACATGT CAAGTGCTAG TGGGTTAGAC ATGCTAAAGA TAGAGGAAAT GGAAAGAATG 
CTTCTAGAAG CTCATGCAGA GCGTGCCAGG CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG 
GCACGCAGAA AAGCACTAGA AGATGAGAGG CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT 
CAAGATGAGA CAGCTCACAG GCAAAGACTA GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG 
CATTTTTCAC AGGCTCGTCC TCTTACACGC TATCTGCCAA TCCGGAAGGA AGACTTTGAT 
CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT GCAGTAGATG CCTGCCCTCA CATCATTTTA 
AGTGAGAAGA TGTGTCGTGG CTTCCTGACA AAAATGGGTG GAAAGATAAA ATCATGGAAG 
AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA CGGACCCTGT CCTATTATGT AGATAAACAT 
GAGGCAAAGC TGAAAGGAGT TATATATTTC CAGGCCATAG AAGAGGTTTA TTATGACCAC 
CTCAGGAGTG CAGCCAAGAA AGGACTTTTT AACCTCAGCC TGGCCACTCA GATATCCGAT 
TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG 
ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG 
GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966183.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821637.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OXa32420
Clone ID Related Accession (Same CDS sequence)	XM_012966183.2 , XM_012966183.3
Accession Version	XM_012966183.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966183.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA 
CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT 
GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG 
GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGGTCAA 
CAAGTGATAG AGGAGCAGCG CAGACGTCTT GCAGAGTTGA AACAGAGAGC TGCAGTTGAG 
GCACAATCTC AGTGGGAATC TCTCCATGGA GCCCCTAATC AAGGGTACAC GCCTATAATT 
CACCACTCCA TTCTACACCA TCAGCGGCCT CTCCATTCTC GTGAAGATGA CCCTACCCAT 
GGATTTGACA CTCTCAGTCT GGAGAGTTCT GATAGTTTGG ACACTAGTGT TTCTACAGGA 
AACTCTGCAT GTTCCCCTGA TAACATGTCA AGTGCTAGTG GGTTAGACAT GCTAAAGATA 
GAGGAAATGG AAAGAATGCT TCTAGAAGCT CATGCAGAGC GTGCCAGGCT TGTAGAATCC 
AGGGAGCGTG AGGTGGAGGC ACGCAGAAAA GCACTAGAAG ATGAGAGGCG AAGGAGAGAG 
GAGCTGGAAA GGCGTCTTCA AGATGAGACA GCTCACAGGC AAAGACTAGT AGAGAAGGAG 
GTCAAGATGA GGGAGAAGCA TTTTTCACAG GCTCGTCCTC TTACACGCTA TCTGCCAATC 
CGGAAGGAAG ACTTTGATCT CCGAGCCCAT GTTGAGTCCT CAGGCCATGC AGTAGATGCC 
TGCCCTCACA TCATTTTAAG TGAGAAGATG TGTCGTGGCT TCCTGACAAA AATGGGTGGA 
AAGATAAAAT CATGGAAGAA GCGCTGGTTT GTTTTTGATC GCCTGAAACG GACCCTGTCC 
TATTATGTAG ATAAACATGA GGCAAAGCTG AAAGGAGTTA TATATTTCCA GGCCATAGAA 
GAGGTTTATT ATGACCACCT CAGGAGTGCA GCCAAGAAAG GACTTTTTAA CCTCAGCCTG 
GCCACTCAGA TATCCGATTT TCTTTCTGTG CCCCTGTGGA AGGAGAGTCC TAACCCAGCT 
CTAACATTCT GTGTCAAGAC TCACGATCGC CTGTATTACA TGGTAGCTCC TTCCCCAGAG 
GCTATGCGGA TATGGATGGA TGTCATTGTA ACTGGGGCAG AGGGTTACAC TCAGTTCATG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821637.1
CDS	35..4000
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966183.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966183.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA 
CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT 
GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG 
GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGGTCAA 
CAAGTGATAG AGGAGCAGCG CAGACGTCTT GCAGAGTTGA AACAGAGAGC TGCAGTTGAG 
GCACAATCTC AGTGGGAATC TCTCCATGGA GCCCCTAATC AAGGGTACAC GCCTATAATT 
CACCACTCCA TTCTACACCA TCAGCGGCCT CTCCATTCTC GTGAAGATGA CCCTACCCAT 
GGATTTGACA CTCTCAGTCT GGAGAGTTCT GATAGTTTGG ACACTAGTGT TTCTACAGGA 
AACTCTGCAT GTTCCCCTGA TAACATGTCA AGTGCTAGTG GGTTAGACAT GCTAAAGATA 
GAGGAAATGG AAAGAATGCT TCTAGAAGCT CATGCAGAGC GTGCCAGGCT TGTAGAATCC 
AGGGAGCGTG AGGTGGAGGC ACGCAGAAAA GCACTAGAAG ATGAGAGGCG AAGGAGAGAG 
GAGCTGGAAA GGCGTCTTCA AGATGAGACA GCTCACAGGC AAAGACTAGT AGAGAAGGAG 
GTCAAGATGA GGGAGAAGCA TTTTTCACAG GCTCGTCCTC TTACACGCTA TCTGCCAATC 
CGGAAGGAAG ACTTTGATCT CCGAGCCCAT GTTGAGTCCT CAGGCCATGC AGTAGATGCC 
TGCCCTCACA TCATTTTAAG TGAGAAGATG TGTCGTGGCT TCCTGACAAA AATGGGTGGA 
AAGATAAAAT CATGGAAGAA GCGCTGGTTT GTTTTTGATC GCCTGAAACG GACCCTGTCC 
TATTATGTAG ATAAACATGA GGCAAAGCTG AAAGGAGTTA TATATTTCCA GGCCATAGAA 
GAGGTTTATT ATGACCACCT CAGGAGTGCA GCCAAGAAAG GACTTTTTAA CCTCAGCCTG 
GCCACTCAGA TATCCGATTT TCTTTCTGTG CCCCTGTGGA AGGAGAGTCC TAACCCAGCT 
CTAACATTCT GTGTCAAGAC TCACGATCGC CTGTATTACA TGGTAGCTCC TTCCCCAGAG 
GCTATGCGGA TATGGATGGA TGTCATTGTA ACTGGGGCAG AGGGTTACAC TCAGTTCATG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966184.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821638.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X23, mRNA.

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CloneID	OXa32421
Clone ID Related Accession (Same CDS sequence)	XM_012966184.2 , XM_012966184.3
Accession Version	XM_012966184.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966184.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821638.1
CDS	35..3871
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966184.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966184.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966185.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821639.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X24, mRNA.

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CloneID	OXa32422
Clone ID Related Accession (Same CDS sequence)	XM_012966185.2 , XM_012966185.3
Accession Version	XM_012966185.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966185.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG 
ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG 
GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821639.1
CDS	35..3802
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966185.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966185.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG 
ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG 
GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966188.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821642.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X25, mRNA.

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CloneID	OXa32424
Clone ID Related Accession (Same CDS sequence)	XM_012966188.3 , XM_012966188.2
Accession Version	XM_012966188.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966188.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGGT 
CAACAAGTGA TAGAGGAGCA GCGCAGACGT CTTGCAGAGT TGAAACAGAG AGCTGCAGTT 
GAGGCACAAT CTCAGTGGGA ATCTCTCCAT GGAGCCCCTA ATCAAGGGTA CACGCCTATA 
ATTCACCACT CCATTCTACA CCATCAGCGG CCTCTCCATT CTCGTGAAGA TGACCCTACC 
CATGGATTTG ACACTCTCAG TCTGGAGAGT TCTGATAGTT TGGACACTAG TGTTTCTACA 
GGAAACTCTG CATGTTCCCC TGATAACATG TCAAGTGCTA GTGGGTTAGA CATGCTAAAG 
ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA GCTCATGCAG AGCGTGCCAG GCTTGTAGAA 
TCCAGGGAGC GTGAGGTGGA GGCACGCAGA AAAGCACTAG AAGATGAGAG GCGAAGGAGA 
GAGGAGCTGG AAAGGCGTCT TCAAGATGAG ACAGCTCACA GGCAAAGACT AGTAGAGAAG 
GAGGTCAAGA TGAGGGAGAA GCATTTTTCA CAGGCTCGTC CTCTTACACG CTATCTGCCA 
ATCCGGAAGG AAGACTTTGA TCTCCGAGCC CATGTTGAGT CCTCAGGCCA TGCAGTAGAT 
GCCTGCCCTC ACATCATTTT AAGTGAGAAG ATGTGTCGTG GCTTCCTGAC AAAAATGGGT 
GGAAAGATAA AATCATGGAA GAAGCGCTGG TTTGTTTTTG ATCGCCTGAA ACGGACCCTG 
TCCTATTATG TAGATAAACA TGAGGCAAAG CTGAAAGGAG TTATATATTT CCAGGCCATA 
GAAGAGGTTT ATTATGACCA CCTCAGGAGT GCAGCCAAGA AAGGACTTTT TAACCTCAGC 
CTGGCCACTC AGATATCCGA TTTTCTTTCT GTGCCCCTGT GGAAGGAGAG TCCTAACCCA 
GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA 
GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC 
ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821642.1
CDS	35..4423
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966188.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966188.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGGT 
CAACAAGTGA TAGAGGAGCA GCGCAGACGT CTTGCAGAGT TGAAACAGAG AGCTGCAGTT 
GAGGCACAAT CTCAGTGGGA ATCTCTCCAT GGAGCCCCTA ATCAAGGGTA CACGCCTATA 
ATTCACCACT CCATTCTACA CCATCAGCGG CCTCTCCATT CTCGTGAAGA TGACCCTACC 
CATGGATTTG ACACTCTCAG TCTGGAGAGT TCTGATAGTT TGGACACTAG TGTTTCTACA 
GGAAACTCTG CATGTTCCCC TGATAACATG TCAAGTGCTA GTGGGTTAGA CATGCTAAAG 
ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA GCTCATGCAG AGCGTGCCAG GCTTGTAGAA 
TCCAGGGAGC GTGAGGTGGA GGCACGCAGA AAAGCACTAG AAGATGAGAG GCGAAGGAGA 
GAGGAGCTGG AAAGGCGTCT TCAAGATGAG ACAGCTCACA GGCAAAGACT AGTAGAGAAG 
GAGGTCAAGA TGAGGGAGAA GCATTTTTCA CAGGCTCGTC CTCTTACACG CTATCTGCCA 
ATCCGGAAGG AAGACTTTGA TCTCCGAGCC CATGTTGAGT CCTCAGGCCA TGCAGTAGAT 
GCCTGCCCTC ACATCATTTT AAGTGAGAAG ATGTGTCGTG GCTTCCTGAC AAAAATGGGT 
GGAAAGATAA AATCATGGAA GAAGCGCTGG TTTGTTTTTG ATCGCCTGAA ACGGACCCTG 
TCCTATTATG TAGATAAACA TGAGGCAAAG CTGAAAGGAG TTATATATTT CCAGGCCATA 
GAAGAGGTTT ATTATGACCA CCTCAGGAGT GCAGCCAAGA AAGGACTTTT TAACCTCAGC 
CTGGCCACTC AGATATCCGA TTTTCTTTCT GTGCCCCTGT GGAAGGAGAG TCCTAACCCA 
GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA 
GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC 
ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_018095290.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950779.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OXa45339
Clone ID Related Accession (Same CDS sequence)	XM_018095290.1
Accession Version	XM_018095290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT 
GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT 
CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG 
ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT 
CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG 
AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG 
AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA 
GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT 
AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950779.1
CDS	61..4338
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_018095290.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095290.1

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT 
GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT 
CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG 
ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT 
CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG 
AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG 
AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA 
GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT 
AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_031904870.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760730.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X8, mRNA.

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CloneID	OXa61273
Clone ID Related Accession (Same CDS sequence)	XM_031904870.1
Accession Version	XM_031904870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTATT TGGAAACAGA ATTCCCTGCT CAGACAGAAA TAGAGGACAC AATGAAGGCA 
CTGAGTCATG ATGTGAGCAG CCCCTGCAGA AAAACACACA CAGTTATTCA GAGCACACCA 
TTGGATCTCA TAGATACAGG AAAAAGTCTT AGGGTGCAGA CAGAAAAACC TCATCTTGTC 
AGCCTGGGAA GTGGACGACT CAGCACTGCA ATCACCCTAC TACCACTGGA AGAAGGAAGG 
ACAACCCTGG GTCATGCATC TAAGGACATT CCCCTGCAGG GACCTGGCAT TGCTGCAGAA 
CATTGCTACA TTGAAAACAT CAGAGGAAAC CTCACCCTAT ACCCATGTGG AAATCCTTGT 
GCCATCGATG GACTGCTCAT CACCCAACCG ACACGTCTCA CTCAAGGGTG TATGGTCTGT 
CTGGGTCAGT CCACCTTCCT GAGATTTAAC CACCCAGCTG AAGCTCACTG GATGAAAACC 
ATGATTCCTT CCACTGGGAG AAGCCCTGCG CCAGGCTTGT ACCCATCAGA TTCTTCTTCC 
TCCTTGCTAA ATGGTTCTCG TGATGCTGCA TACCCGAGAA CTGTACCTTC CCATACCTCA 
CTAGTAAGCT CCATTGAAAA AAATCTTCAG GACATCATGG ACTCGTTGGT TTTAGAAGGG 
CCTGCACCAC TGAGCAGGAA GCTTTCCCAG ACCTCACTTA ACCATATCAC CCCTATGACC 
AATGGAGAGA ACAGACGGTA CTTGCTTTCA TCTCCAACAA GTCCACCTTA CTCAAACTCA 
GGTTATGACA ATACCTCTCC ACTGTCTTCT CCTGGAAGTG GAAGTCTCAC TAGCCCCTCT 
CCGGGTCAAG ATGCAGGAAC GCCAACAGCA CCCCCTGTGG TGCCACTTCG AACTTCTAGC 
TTTAGCCATT CCTTGCCACG ACCAGTTCCT ACCTATGGAG GAAGCAACCT TGACTTGCGT 
TCTAATCGCA ACACAGGTGG TGGGTCTCTG CTTAGTCCTT CAAGCCCAGC AACAGGAAGC 
AGGATGGGGG GACTAAGTGT GCCAAGCAGT CCACTCCTAG GTAGTAAGTT TCATCACCAG 
ATCACAGACC GCCCAACCAG CCCTTTTAGA CAGGTGGTAG GACCAACACG GAGTCTGCCA 
CCAGAGAGTC CAAGACTTGG GCGGAGGAAC TTTGAAAGCA TGAGGGACCT GCCTCCACTT 
AGCCCTTCTC TGCCCCGAAG AAGTTCATCT AGCTCCATTA AACCTGCTCC AGACAGCCCA 
TCTCGAGTTC CAGACAGCCC CAGCACTTTA GGGCGACGTC GGGTGGGAAG TGCTAGCTCT 
CCTGGCACAG AAAATAATGC AAGTACAAGG CAGATAAGGG AGCGCAGCCC ATCTCCCTCC 
TCTCTTCAAG AGACCTCTCG TCGACTAACT CCAGCTTATA GCCTGGGATC GCTTGGGACC 
CCTTCACAAA GCCCCAGGGC ACAAAGAAAA GGGCCTGTGC TACGGGAGCG CAAGAACAGC 
ATCAGTGAAA TTTCTGGCAA CCAAGAGGAG CTAATGGACT ACCACAGAAG GCAGAGAGAA 
GAAAGACTTA GAGAGCAGGA AATGGAAAGG CTGGAGCGGC AGAGACTGGA AACCATTTTA 
AACTTGTGTG CCGAATACAG TAAAACAGAT GATACAGCTC CCCCAGCTCG CCTAGATGAA 
ATAAGGAGCT TCCCCACTTT GGGGGGTACT GAGGAAACCA CTGCATCCCT CTTCACTCGT 
AGACCTTCTT GGGAGAGCAC CAGAAGTACC AACCTGGAGG GAACAGGCAA TAGGGTGAGA 
GCACAGGGAG AACACAATGA GGAAGAGACT CAGAGAGAAG AGAACAGCAG TACAGAGAGT 
ACCCAACATG AGCAGGAAGA TGCTGTAAGC CCAACTGGGG CTGCAGAATT ACTAGCCCTG 
GAGGAGCAAC GCAGGCACAC CCTGGGGCAT GTGGATCAGC TAAAGAATAG AGTGACTGAA 
TTGGAGGGTC AGTTGCAGGA AAGTGCAAGG GAGGCAGAGA TGGAACGTGC CCTTTTGCAA 
GGGGAACGGG AGGCTGAGCT GTCCCAGTTG CAGCATGAGC AAAGAATGGT CCAGCAGCTG 
CAGGACAGCC TGACCTCTTT AGAAAACAAC TTGCAGAAGG AGAAAGACAA GGCACGTGAG 
AAACTGGAGC AGGAGAGGAT GGAGCTGCAG AGTATTCAGC TTTCTCACCA CGACCTGCAG 
AGGCAGCTCC ATAACTGCCC TGAGGCTCTG CGGGAGCAGG TGCAGTGCCA GCTGACACGG 
GAGTCTGAAC TCCTGGAGAC GAAGATGAAG CTGTTTGAGG ATCTGGAGTT CCAGCAGCTA 
GAAAAGGAAA GCCGCCTGGA AGAAGAAAGA GAGACCACAA GTCAACAACT TTTACAGAGC 
AAGGGGGAAT GCCAGCGAAG CATTGCACGC AGGAAGGAAC GCCTGGCTGC TTTGGAGATG 
CAAGCCAATC AGATTCGCCT CCAGGCAGCA CAGGATGCAG ACCATCTTGG CCAGGAAAGA 
AGCGCCATAC TTTTACAGCT ACACAAGGAG AAAGAGAAGC TGGTTCAGTT GGAGAGAAGA 
TACCAGGCAC TAACAGGACG ACGGGGATTC TCAACTGGAT CAGCTGCTCT CCGAGAGGAT 
GCTCTTCAAA TCTATGAGAC TAGAGACTGC TTACTCGCTC AAGCTGATGT TTCTTGCACA 
CCTGCTATCC CTTCTGCCTG CTCTCACTCC CCTTCCCGGC AGCAAGAGGA ATACCTGTGC 
CTCTCTGATG TTCTCCGCTT GTACAGCAGC TCGCCTCTTG TTCCTGCTTT TCCCCTTCTC 
TCTGCTGCAG AGCCCACCGC CGCCCTTGAG TACATCACTT TAGATCAGCT GCAGGATATT 
CTGGAAAGCA TGCCCACAGG TTCTGCTCCA ACTGCTGTGG CAATGGCTGG CACTGATATC 
AACTCTGCCT CTTGTGCTCC TTCACCTGAC GACCCTCCTC CTGTACATCT CTCGTCTTCA 
TCTTCTTCCT CTCCCTCCAT TTCTCTGGAG GTGAATTCCC CAACAACAGA AGGTCACTCT 
GCCCCCCTAG CACCTGATTT GGATGCCTAT TACCAAGATT TAGTAACTGC ACCTTCTGCC 
TCTTCTTCTC CATCTTCTTC CCCTCCTCCA CTCCCTGCTA AATCCCACTC ATCACGTAAG 
GCTTTGCAGA TTTCTCGCTC TCGGACAGAA ATTGAGGGTG GAGGCACTCT TCCACGGATG 
AAAACAGTCT CCCCCGCTTC TTCTCACTTT TCAGTGGCCA CTCTGGGCCG TAGTGTTTCC 
CCAAAGAATG CCCATCTGAC TCAGAATGGC ACAGGCAGCC TCTCCAGGGG ATTGGCAGTA 
ACCATGCAAG ACATTCAAAG CAAGAGACAG CTGGCACTGC AACAAAAAGA CAGTCTGAGC 
ACAGAAGATT CTCTTCCATC TGAAGTTGAA GGTCAACAAG TGATAGAGGA GCAGCGCAGA 
CGTCTTGCAG AGTTGAAACA GAGAGCTGCA GTTGAGGCAC AATCTCAGTG GGAATCTCTC 
CATGGAGCCC CTAATCAAGG GTACACGCCT ATAATTCACC ACTCCATTCT ACACCATCAG 
CGGCCTCTCC ATTCTCGTGA AGATGACCCT ACCCATGGAT TTGACACTCT CAGTCTGGAG 
AGTTCTGATA GTTTGGACAC TAGTGTTTCT ACAGGAAACT CTGCATGTTC CCCTGATAAC 
ATGTCAAGTG CTAGTGGGTT AGACATGCTA AAGATAGAGG AAATGGAAAG AATGCTTCTA 
GAAGCTCATG CAGAGCGTGC CAGGCTTGTA GAATCCAGGG AGCGTGAGGT GGAGGCACGC 
AGAAAAGCAC TAGAAGATGA GAGGCGAAGG AGAGAGGAGC TGGAAAGGCG TCTTCAAGAT 
GAGACAGCTC ACAGGCAAAG ACTAGTAGAG AAGGAGGTCA AGATGAGGGA GAAGCATTTT 
TCACAGGCTC GTCCTCTTAC ACGCTATCTG CCAATCCGGA AGGAAGACTT TGATCTCCGA 
GCCCATGTTG AGTCCTCAGG CCATGCAGTA GATGCCTGCC CTCACATCAT TTTAAGTGAG 
AAGATGTGTC GTGGCTTCCT GACAAAAATG GGTGGAAAGA TAAAATCATG GAAGAAGCGC 
TGGTTTGTTT TTGATCGCCT GAAACGGACC CTGTCCTATT ATGTAGATAA ACATGAGGCA 
AAGCTGAAAG GAGTTATATA TTTCCAGGCC ATAGAAGAGG TTTATTATGA CCACCTCAGG 
AGTGCAGCCA AGAAAGGACT TTTTAACCTC AGCCTGGCCA CTCAGATATC CGATTTTCTT 
TCTGTGCCCC TGTGGAAGGA GAGTCCTAAC CCAGCTCTAA CATTCTGTGT CAAGACTCAC 
GATCGCCTGT ATTACATGGT AGCTCCTTCC CCAGAGGCTA TGCGGATATG GATGGATGTC 
ATTGTAACTG GGGCAGAGGG TTACACTCAG TTCATGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760730.1
CDS	16..4497
Translation	MDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_031904870.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904870.1

ORF Insert Sequence:

ATGGATTATT TGGAAACAGA ATTCCCTGCT CAGACAGAAA TAGAGGACAC AATGAAGGCA 
CTGAGTCATG ATGTGAGCAG CCCCTGCAGA AAAACACACA CAGTTATTCA GAGCACACCA 
TTGGATCTCA TAGATACAGG AAAAAGTCTT AGGGTGCAGA CAGAAAAACC TCATCTTGTC 
AGCCTGGGAA GTGGACGACT CAGCACTGCA ATCACCCTAC TACCACTGGA AGAAGGAAGG 
ACAACCCTGG GTCATGCATC TAAGGACATT CCCCTGCAGG GACCTGGCAT TGCTGCAGAA 
CATTGCTACA TTGAAAACAT CAGAGGAAAC CTCACCCTAT ACCCATGTGG AAATCCTTGT 
GCCATCGATG GACTGCTCAT CACCCAACCG ACACGTCTCA CTCAAGGGTG TATGGTCTGT 
CTGGGTCAGT CCACCTTCCT GAGATTTAAC CACCCAGCTG AAGCTCACTG GATGAAAACC 
ATGATTCCTT CCACTGGGAG AAGCCCTGCG CCAGGCTTGT ACCCATCAGA TTCTTCTTCC 
TCCTTGCTAA ATGGTTCTCG TGATGCTGCA TACCCGAGAA CTGTACCTTC CCATACCTCA 
CTAGTAAGCT CCATTGAAAA AAATCTTCAG GACATCATGG ACTCGTTGGT TTTAGAAGGG 
CCTGCACCAC TGAGCAGGAA GCTTTCCCAG ACCTCACTTA ACCATATCAC CCCTATGACC 
AATGGAGAGA ACAGACGGTA CTTGCTTTCA TCTCCAACAA GTCCACCTTA CTCAAACTCA 
GGTTATGACA ATACCTCTCC ACTGTCTTCT CCTGGAAGTG GAAGTCTCAC TAGCCCCTCT 
CCGGGTCAAG ATGCAGGAAC GCCAACAGCA CCCCCTGTGG TGCCACTTCG AACTTCTAGC 
TTTAGCCATT CCTTGCCACG ACCAGTTCCT ACCTATGGAG GAAGCAACCT TGACTTGCGT 
TCTAATCGCA ACACAGGTGG TGGGTCTCTG CTTAGTCCTT CAAGCCCAGC AACAGGAAGC 
AGGATGGGGG GACTAAGTGT GCCAAGCAGT CCACTCCTAG GTAGTAAGTT TCATCACCAG 
ATCACAGACC GCCCAACCAG CCCTTTTAGA CAGGTGGTAG GACCAACACG GAGTCTGCCA 
CCAGAGAGTC CAAGACTTGG GCGGAGGAAC TTTGAAAGCA TGAGGGACCT GCCTCCACTT 
AGCCCTTCTC TGCCCCGAAG AAGTTCATCT AGCTCCATTA AACCTGCTCC AGACAGCCCA 
TCTCGAGTTC CAGACAGCCC CAGCACTTTA GGGCGACGTC GGGTGGGAAG TGCTAGCTCT 
CCTGGCACAG AAAATAATGC AAGTACAAGG CAGATAAGGG AGCGCAGCCC ATCTCCCTCC 
TCTCTTCAAG AGACCTCTCG TCGACTAACT CCAGCTTATA GCCTGGGATC GCTTGGGACC 
CCTTCACAAA GCCCCAGGGC ACAAAGAAAA GGGCCTGTGC TACGGGAGCG CAAGAACAGC 
ATCAGTGAAA TTTCTGGCAA CCAAGAGGAG CTAATGGACT ACCACAGAAG GCAGAGAGAA 
GAAAGACTTA GAGAGCAGGA AATGGAAAGG CTGGAGCGGC AGAGACTGGA AACCATTTTA 
AACTTGTGTG CCGAATACAG TAAAACAGAT GATACAGCTC CCCCAGCTCG CCTAGATGAA 
ATAAGGAGCT TCCCCACTTT GGGGGGTACT GAGGAAACCA CTGCATCCCT CTTCACTCGT 
AGACCTTCTT GGGAGAGCAC CAGAAGTACC AACCTGGAGG GAACAGGCAA TAGGGTGAGA 
GCACAGGGAG AACACAATGA GGAAGAGACT CAGAGAGAAG AGAACAGCAG TACAGAGAGT 
ACCCAACATG AGCAGGAAGA TGCTGTAAGC CCAACTGGGG CTGCAGAATT ACTAGCCCTG 
GAGGAGCAAC GCAGGCACAC CCTGGGGCAT GTGGATCAGC TAAAGAATAG AGTGACTGAA 
TTGGAGGGTC AGTTGCAGGA AAGTGCAAGG GAGGCAGAGA TGGAACGTGC CCTTTTGCAA 
GGGGAACGGG AGGCTGAGCT GTCCCAGTTG CAGCATGAGC AAAGAATGGT CCAGCAGCTG 
CAGGACAGCC TGACCTCTTT AGAAAACAAC TTGCAGAAGG AGAAAGACAA GGCACGTGAG 
AAACTGGAGC AGGAGAGGAT GGAGCTGCAG AGTATTCAGC TTTCTCACCA CGACCTGCAG 
AGGCAGCTCC ATAACTGCCC TGAGGCTCTG CGGGAGCAGG TGCAGTGCCA GCTGACACGG 
GAGTCTGAAC TCCTGGAGAC GAAGATGAAG CTGTTTGAGG ATCTGGAGTT CCAGCAGCTA 
GAAAAGGAAA GCCGCCTGGA AGAAGAAAGA GAGACCACAA GTCAACAACT TTTACAGAGC 
AAGGGGGAAT GCCAGCGAAG CATTGCACGC AGGAAGGAAC GCCTGGCTGC TTTGGAGATG 
CAAGCCAATC AGATTCGCCT CCAGGCAGCA CAGGATGCAG ACCATCTTGG CCAGGAAAGA 
AGCGCCATAC TTTTACAGCT ACACAAGGAG AAAGAGAAGC TGGTTCAGTT GGAGAGAAGA 
TACCAGGCAC TAACAGGACG ACGGGGATTC TCAACTGGAT CAGCTGCTCT CCGAGAGGAT 
GCTCTTCAAA TCTATGAGAC TAGAGACTGC TTACTCGCTC AAGCTGATGT TTCTTGCACA 
CCTGCTATCC CTTCTGCCTG CTCTCACTCC CCTTCCCGGC AGCAAGAGGA ATACCTGTGC 
CTCTCTGATG TTCTCCGCTT GTACAGCAGC TCGCCTCTTG TTCCTGCTTT TCCCCTTCTC 
TCTGCTGCAG AGCCCACCGC CGCCCTTGAG TACATCACTT TAGATCAGCT GCAGGATATT 
CTGGAAAGCA TGCCCACAGG TTCTGCTCCA ACTGCTGTGG CAATGGCTGG CACTGATATC 
AACTCTGCCT CTTGTGCTCC TTCACCTGAC GACCCTCCTC CTGTACATCT CTCGTCTTCA 
TCTTCTTCCT CTCCCTCCAT TTCTCTGGAG GTGAATTCCC CAACAACAGA AGGTCACTCT 
GCCCCCCTAG CACCTGATTT GGATGCCTAT TACCAAGATT TAGTAACTGC ACCTTCTGCC 
TCTTCTTCTC CATCTTCTTC CCCTCCTCCA CTCCCTGCTA AATCCCACTC ATCACGTAAG 
GCTTTGCAGA TTTCTCGCTC TCGGACAGAA ATTGAGGGTG GAGGCACTCT TCCACGGATG 
AAAACAGTCT CCCCCGCTTC TTCTCACTTT TCAGTGGCCA CTCTGGGCCG TAGTGTTTCC 
CCAAAGAATG CCCATCTGAC TCAGAATGGC ACAGGCAGCC TCTCCAGGGG ATTGGCAGTA 
ACCATGCAAG ACATTCAAAG CAAGAGACAG CTGGCACTGC AACAAAAAGA CAGTCTGAGC 
ACAGAAGATT CTCTTCCATC TGAAGTTGAA GGTCAACAAG TGATAGAGGA GCAGCGCAGA 
CGTCTTGCAG AGTTGAAACA GAGAGCTGCA GTTGAGGCAC AATCTCAGTG GGAATCTCTC 
CATGGAGCCC CTAATCAAGG GTACACGCCT ATAATTCACC ACTCCATTCT ACACCATCAG 
CGGCCTCTCC ATTCTCGTGA AGATGACCCT ACCCATGGAT TTGACACTCT CAGTCTGGAG 
AGTTCTGATA GTTTGGACAC TAGTGTTTCT ACAGGAAACT CTGCATGTTC CCCTGATAAC 
ATGTCAAGTG CTAGTGGGTT AGACATGCTA AAGATAGAGG AAATGGAAAG AATGCTTCTA 
GAAGCTCATG CAGAGCGTGC CAGGCTTGTA GAATCCAGGG AGCGTGAGGT GGAGGCACGC 
AGAAAAGCAC TAGAAGATGA GAGGCGAAGG AGAGAGGAGC TGGAAAGGCG TCTTCAAGAT 
GAGACAGCTC ACAGGCAAAG ACTAGTAGAG AAGGAGGTCA AGATGAGGGA GAAGCATTTT 
TCACAGGCTC GTCCTCTTAC ACGCTATCTG CCAATCCGGA AGGAAGACTT TGATCTCCGA 
GCCCATGTTG AGTCCTCAGG CCATGCAGTA GATGCCTGCC CTCACATCAT TTTAAGTGAG 
AAGATGTGTC GTGGCTTCCT GACAAAAATG GGTGGAAAGA TAAAATCATG GAAGAAGCGC 
TGGTTTGTTT TTGATCGCCT GAAACGGACC CTGTCCTATT ATGTAGATAA ACATGAGGCA 
AAGCTGAAAG GAGTTATATA TTTCCAGGCC ATAGAAGAGG TTTATTATGA CCACCTCAGG 
AGTGCAGCCA AGAAAGGACT TTTTAACCTC AGCCTGGCCA CTCAGATATC CGATTTTCTT 
TCTGTGCCCC TGTGGAAGGA GAGTCCTAAC CCAGCTCTAA CATTCTGTGT CAAGACTCAC 
GATCGCCTGT ATTACATGGT AGCTCCTTCC CCAGAGGCTA TGCGGATATG GATGGATGTC 
ATTGTAACTG GGGCAGAGGG TTACACTCAG TTCATGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_031904871.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760731.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OXa61274
Clone ID Related Accession (Same CDS sequence)	XM_031904871.1
Accession Version	XM_031904871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGTACAT CACTTTAGAT CAGCTGCAGG ATATTCTGGA AAGCATGCCC 
ACAGGTTCTG CTCCAACTGC TGTGGCAATG GCTGGCACTG ATATCAACTC TGCCTCTTGT 
GCTCCTTCAC CTGACGACCC TCCTCCTGTA CATCTCTCGT CTTCATCTTC TTCCTCTCCC 
TCCATTTCTC TGGAGGTGAA TTCCCCAACA ACAGAAGGTC ACTCTGCCCC CCTAGCACCT 
GATTTGGATG CCTATTACCA AGATTTAGTA ACTGCACCTT CTGCCTCTTC TTCTCCATCT 
TCTTCCCCTC CTCCACTCCC TGCTAAATCC CACTCATCAC GTAAGGCTTT GCAGATTTCT 
CGCTCTCGGA CAGAAATTGA GGGTGGAGGC ACTCTTCCAC GGATGAAAAC AGTCTCCCCC 
GCTTCTTCTC ACTTTTCAGT GGCCACTCTG GGCCGTAGTG TTTCCCCAAA GAATGCCCAT 
CTGACTCAGA ATGGCACAGG CAGCCTCTCC AGGGGATTGG CAGTAACCAT GCAAGACATT 
CAAAGCAAGA GACAGCTGGC ACTGCAACAA AAAGACAGTC TGAGCACAGA AGATTCTCTT 
CCATCTGAAG TTGAAGGTCA ACAAGTGATA GAGGAGCAGC GCAGACGTCT TGCAGAGTTG 
AAACAGAGAG CTGCAGTTGA GGCACAATCT CAGTGGGAAT CTCTCCATGG AGCCCCTAAT 
CAAGGGTACA CGCCTATAAT TCACCACTCC ATTCTACACC ATCAGCGGCC TCTCCATTCT 
CGTGAAGATG ACCCTACCCA TGGATTTGAC ACTCTCAGTC TGGAGAGTTC TGATAGTTTG 
GACACTAGTG TTTCTACAGG AAACTCTGCA TGTTCCCCTG ATAACATGTC AAGTGCTAGT 
GGGTTAGACA TGCTAAAGAT AGAGGAAATG GAAAGAATGC TTCTAGAAGC TCATGCAGAG 
CGTGCCAGGC TTGTAGAATC CAGGGAGCGT GAGGTGGAGG CACGCAGAAA AGCACTAGAA 
GATGAGAGGC GAAGGAGAGA GGAGCTGGAA AGGCGTCTTC AAGATGAGAC AGCTCACAGG 
CAAAGACTAG TAGAGAAGGA GGTCAAGATG AGGGAGAAGC ATTTTTCACA GGCTCGTCCT 
CTTACACGCT ATCTGCCAAT CCGGAAGGAA GACTTTGATC TCCGAGCCCA TGTTGAGTCC 
TCAGGCCATG CAGTAGATGC CTGCCCTCAC ATCATTTTAA GTGAGAAGAT GTGTCGTGGC 
TTCCTGACAA AAATGGGTGG AAAGATAAAA TCATGGAAGA AGCGCTGGTT TGTTTTTGAT 
CGCCTGAAAC GGACCCTGTC CTATTATGTA GATAAACATG AGGCAAAGCT GAAAGGAGTT 
ATATATTTCC AGGCCATAGA AGAGGTTTAT TATGACCACC TCAGGAGTGC AGCCAAGAAA 
GGACTTTTTA ACCTCAGCCT GGCCACTCAG ATATCCGATT TTCTTTCTGT GCCCCTGTGG 
AAGGAGAGTC CTAACCCAGC TCTAACATTC TGTGTCAAGA CTCACGATCG CCTGTATTAC 
ATGGTAGCTC CTTCCCCAGA GGCTATGCGG ATATGGATGG ATGTCATTGT AACTGGGGCA 
GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760731.1
CDS	35..4381
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_031904871.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904871.1

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGTACAT CACTTTAGAT CAGCTGCAGG ATATTCTGGA AAGCATGCCC 
ACAGGTTCTG CTCCAACTGC TGTGGCAATG GCTGGCACTG ATATCAACTC TGCCTCTTGT 
GCTCCTTCAC CTGACGACCC TCCTCCTGTA CATCTCTCGT CTTCATCTTC TTCCTCTCCC 
TCCATTTCTC TGGAGGTGAA TTCCCCAACA ACAGAAGGTC ACTCTGCCCC CCTAGCACCT 
GATTTGGATG CCTATTACCA AGATTTAGTA ACTGCACCTT CTGCCTCTTC TTCTCCATCT 
TCTTCCCCTC CTCCACTCCC TGCTAAATCC CACTCATCAC GTAAGGCTTT GCAGATTTCT 
CGCTCTCGGA CAGAAATTGA GGGTGGAGGC ACTCTTCCAC GGATGAAAAC AGTCTCCCCC 
GCTTCTTCTC ACTTTTCAGT GGCCACTCTG GGCCGTAGTG TTTCCCCAAA GAATGCCCAT 
CTGACTCAGA ATGGCACAGG CAGCCTCTCC AGGGGATTGG CAGTAACCAT GCAAGACATT 
CAAAGCAAGA GACAGCTGGC ACTGCAACAA AAAGACAGTC TGAGCACAGA AGATTCTCTT 
CCATCTGAAG TTGAAGGTCA ACAAGTGATA GAGGAGCAGC GCAGACGTCT TGCAGAGTTG 
AAACAGAGAG CTGCAGTTGA GGCACAATCT CAGTGGGAAT CTCTCCATGG AGCCCCTAAT 
CAAGGGTACA CGCCTATAAT TCACCACTCC ATTCTACACC ATCAGCGGCC TCTCCATTCT 
CGTGAAGATG ACCCTACCCA TGGATTTGAC ACTCTCAGTC TGGAGAGTTC TGATAGTTTG 
GACACTAGTG TTTCTACAGG AAACTCTGCA TGTTCCCCTG ATAACATGTC AAGTGCTAGT 
GGGTTAGACA TGCTAAAGAT AGAGGAAATG GAAAGAATGC TTCTAGAAGC TCATGCAGAG 
CGTGCCAGGC TTGTAGAATC CAGGGAGCGT GAGGTGGAGG CACGCAGAAA AGCACTAGAA 
GATGAGAGGC GAAGGAGAGA GGAGCTGGAA AGGCGTCTTC AAGATGAGAC AGCTCACAGG 
CAAAGACTAG TAGAGAAGGA GGTCAAGATG AGGGAGAAGC ATTTTTCACA GGCTCGTCCT 
CTTACACGCT ATCTGCCAAT CCGGAAGGAA GACTTTGATC TCCGAGCCCA TGTTGAGTCC 
TCAGGCCATG CAGTAGATGC CTGCCCTCAC ATCATTTTAA GTGAGAAGAT GTGTCGTGGC 
TTCCTGACAA AAATGGGTGG AAAGATAAAA TCATGGAAGA AGCGCTGGTT TGTTTTTGAT 
CGCCTGAAAC GGACCCTGTC CTATTATGTA GATAAACATG AGGCAAAGCT GAAAGGAGTT 
ATATATTTCC AGGCCATAGA AGAGGTTTAT TATGACCACC TCAGGAGTGC AGCCAAGAAA 
GGACTTTTTA ACCTCAGCCT GGCCACTCAG ATATCCGATT TTCTTTCTGT GCCCCTGTGG 
AAGGAGAGTC CTAACCCAGC TCTAACATTC TGTGTCAAGA CTCACGATCG CCTGTATTAC 
ATGGTAGCTC CTTCCCCAGA GGCTATGCGG ATATGGATGG ATGTCATTGT AACTGGGGCA 
GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_031904872.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760732.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OXa61275
Clone ID Related Accession (Same CDS sequence)	XM_031904872.1
Accession Version	XM_031904872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA 
GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG 
AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA 
CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA 
ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA 
GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT 
CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG 
TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG 
GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA 
CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG 
GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT 
CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT 
CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT 
GCTCTCCGAG AGTACATCAC TTTAGATCAG CTGCAGGATA TTCTGGAAAG CATGCCCACA 
GGTTCTGCTC CAACTGCTGT GGCAATGGCT GGCACTGATA TCAACTCTGC CTCTTGTGCT 
CCTTCACCTG ACGACCCTCC TCCTGTACAT CTCTCGTCTT CATCTTCTTC CTCTCCCTCC 
ATTTCTCTGG AGGTGAATTC CCCAACAACA GAAGGTCACT CTGCCCCCCT AGCACCTGAT 
TTGGATGCCT ATTACCAAGA TTTAGTAACT GCACCTTCTG CCTCTTCTTC TCCATCTTCT 
TCCCCTCCTC CACTCCCTGC TAAATCCCAC TCATCACGTA AGGCTTTGCA GATTTCTCGC 
TCTCGGACAG AAATTGAGGG TGGAGGCACT CTTCCACGGA TGAAAACAGT CTCCCCCGCT 
TCTTCTCACT TTTCAGTGGC CACTCTGGGC CGTAGTGTTT CCCCAAAGAA TGCCCATCTG 
ACTCAGAATG GCACAGGCAG CCTCTCCAGG GGATTGGCAG TAACCATGCA AGACATTCAA 
AGCAAGAGAC AGCTGGCACT GCAACAAAAA GGTCAACAAG TGATAGAGGA GCAGCGCAGA 
CGTCTTGCAG AGTTGAAACA GAGAGCTGCA GTTGAGGCAC AATCTCAGTG GGAATCTCTC 
CATGGAGCCC CTAATCAAGG GTACACGCCT ATAATTCACC ACTCCATTCT ACACCATCAG 
CGGCCTCTCC ATTCTCGTGA AGATGACCCT ACCCATGGAT TTGACACTCT CAGTCTGGAG 
AGTTCTGATA GTTTGGACAC TAGTGTTTCT ACAGGAAACT CTGCATGTTC CCCTGATAAC 
ATGTCAAGTG CTAGTGGGTT AGACATGCTA AAGATAGAGG AAATGGAAAG AATGCTTCTA 
GAAGCTCATG CAGAGCGTGC CAGGCTTGTA GAATCCAGGG AGCGTGAGGT GGAGGCACGC 
AGAAAAGCAC TAGAAGATGA GAGGCGAAGG AGAGAGGAGC TGGAAAGGCG TCTTCAAGAT 
GAGACAGCTC ACAGGCAAAG ACTAGTAGAG AAGGAGGTCA AGATGAGGGA GAAGCATTTT 
TCACAGGCTC GTCCTCTTAC ACGCTATCTG CCAATCCGGA AGGAAGACTT TGATCTCCGA 
GCCCATGTTG AGTCCTCAGG CCATGCAGTA GATGCCTGCC CTCACATCAT TTTAAGTGAG 
AAGATGTGTC GTGGCTTCCT GACAAAAATG GGTGGAAAGA TAAAATCATG GAAGAAGCGC 
TGGTTTGTTT TTGATCGCCT GAAACGGACC CTGTCCTATT ATGTAGATAA ACATGAGGCA 
AAGCTGAAAG GAGTTATATA TTTCCAGGCC ATAGAAGAGG TTTATTATGA CCACCTCAGG 
AGTGCAGCCA AGAGTCCTAA CCCAGCTCTA ACATTCTGTG TCAAGACTCA CGATCGCCTG 
TATTACATGG TAGCTCCTTC CCCAGAGGCT ATGCGGATAT GGATGGATGT CATTGTAACT 
GGGGCAGAGG GTTACACTCA GTTCATGAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760732.1
CDS	35..4267
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_031904872.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904872.1

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA 
GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG 
AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA 
CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA 
ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA 
GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT 
CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG 
TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG 
GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA 
CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG 
GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT 
CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT 
CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT 
GCTCTCCGAG AGTACATCAC TTTAGATCAG CTGCAGGATA TTCTGGAAAG CATGCCCACA 
GGTTCTGCTC CAACTGCTGT GGCAATGGCT GGCACTGATA TCAACTCTGC CTCTTGTGCT 
CCTTCACCTG ACGACCCTCC TCCTGTACAT CTCTCGTCTT CATCTTCTTC CTCTCCCTCC 
ATTTCTCTGG AGGTGAATTC CCCAACAACA GAAGGTCACT CTGCCCCCCT AGCACCTGAT 
TTGGATGCCT ATTACCAAGA TTTAGTAACT GCACCTTCTG CCTCTTCTTC TCCATCTTCT 
TCCCCTCCTC CACTCCCTGC TAAATCCCAC TCATCACGTA AGGCTTTGCA GATTTCTCGC 
TCTCGGACAG AAATTGAGGG TGGAGGCACT CTTCCACGGA TGAAAACAGT CTCCCCCGCT 
TCTTCTCACT TTTCAGTGGC CACTCTGGGC CGTAGTGTTT CCCCAAAGAA TGCCCATCTG 
ACTCAGAATG GCACAGGCAG CCTCTCCAGG GGATTGGCAG TAACCATGCA AGACATTCAA 
AGCAAGAGAC AGCTGGCACT GCAACAAAAA GGTCAACAAG TGATAGAGGA GCAGCGCAGA 
CGTCTTGCAG AGTTGAAACA GAGAGCTGCA GTTGAGGCAC AATCTCAGTG GGAATCTCTC 
CATGGAGCCC CTAATCAAGG GTACACGCCT ATAATTCACC ACTCCATTCT ACACCATCAG 
CGGCCTCTCC ATTCTCGTGA AGATGACCCT ACCCATGGAT TTGACACTCT CAGTCTGGAG 
AGTTCTGATA GTTTGGACAC TAGTGTTTCT ACAGGAAACT CTGCATGTTC CCCTGATAAC 
ATGTCAAGTG CTAGTGGGTT AGACATGCTA AAGATAGAGG AAATGGAAAG AATGCTTCTA 
GAAGCTCATG CAGAGCGTGC CAGGCTTGTA GAATCCAGGG AGCGTGAGGT GGAGGCACGC 
AGAAAAGCAC TAGAAGATGA GAGGCGAAGG AGAGAGGAGC TGGAAAGGCG TCTTCAAGAT 
GAGACAGCTC ACAGGCAAAG ACTAGTAGAG AAGGAGGTCA AGATGAGGGA GAAGCATTTT 
TCACAGGCTC GTCCTCTTAC ACGCTATCTG CCAATCCGGA AGGAAGACTT TGATCTCCGA 
GCCCATGTTG AGTCCTCAGG CCATGCAGTA GATGCCTGCC CTCACATCAT TTTAAGTGAG 
AAGATGTGTC GTGGCTTCCT GACAAAAATG GGTGGAAAGA TAAAATCATG GAAGAAGCGC 
TGGTTTGTTT TTGATCGCCT GAAACGGACC CTGTCCTATT ATGTAGATAA ACATGAGGCA 
AAGCTGAAAG GAGTTATATA TTTCCAGGCC ATAGAAGAGG TTTATTATGA CCACCTCAGG 
AGTGCAGCCA AGAGTCCTAA CCCAGCTCTA ACATTCTGTG TCAAGACTCA CGATCGCCTG 
TATTACATGG TAGCTCCTTC CCCAGAGGCT ATGCGGATAT GGATGGATGT CATTGTAACT 
GGGGCAGAGG GTTACACTCA GTTCATGAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_031904873.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760733.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X16, mRNA.

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CloneID	OXa61276
Clone ID Related Accession (Same CDS sequence)	XM_031904873.1
Accession Version	XM_031904873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCTC TCCTCCCTCT CTTCCCCTCG CCACATCCTG CCCAAATTAC AGAAAAAGGC 
AGAGCAGATT ATTTGGAAAC AGAATTCCCT GCTCAGACAG AAATAGAGGA CACAATGAAG 
GCACTGAGTC ATGATGTGAG CAGCCCCTGC AGAAAAACAC ACACAGTTAT TCAGAGCACA 
CCATTGGATC TCATAGATAC AGGAAAAAGT CTTAGGGTGC AGACAGAAAA ACCTCATCTT 
GTCAGCCTGG GAAGTGGACG ACTCAGCACT GCAATCACCC TACTACCACT GGAAGAAGGA 
AGGACAACCC TGGGTCATGC ATCTAAGGAC ATTCCCCTGC AGGGACCTGG CATTGCTGCA 
GAACATTGCT ACATTGAAAA CATCAGAGGA AACCTCACCC TATACCCATG TGGAAATCCT 
TGTGCCATCG ATGGACTGCT CATCACCCAA CCGACACGTC TCACTCAAGG GTGTATGGTC 
TGTCTGGGTC AGTCCACCTT CCTGAGATTT AACCACCCAG CTGAAGCTCA CTGGATGAAA 
ACCATGATTC CTTCCACTGG GAGAAGCCCT GCGCCAGGCT TGTACCCATC AGATTCTTCT 
TCCTCCTTGC TAAATGGTTC TCGTGATGCT GCATACCCGA GAACTGTACC TTCCCATACC 
TCACTAGTAA GCTCCATTGA AAAAAATCTT CAGGACATCA TGGACTCGTT GGTTTTAGAA 
GGGCCTGCAC CACTGAGCAG GAAGCTTTCC CAGACCTCAC TTAACCATAT CACCCCTATG 
ACCAATGGAG AGAACAGACG GTACTTGCTT TCATCTCCAA CAAGTCCACC TTACTCAAAC 
TCAGGTTATG ACAATACCTC TCCACTGTCT TCTCCTGGAA GTGGAAGTCT CACTAGCCCC 
TCTCCGGGTC AAGATGCAGG AACGCCAACA GCACCCCCTG TGGTGCCACT TCGAACTTCT 
AGCTTTAGCC ATTCCTTGCC ACGACCAGTT CCTACCTATG GAGGAAGCAA CCTTGACTTG 
CGTTCTAATC GCAACACAGG TGGTGGGTCT CTGCTTAGTC CTTCAAGCCC AGCAACAGGA 
AGCAGGATGG GGGGACTAAG TGTGCCAAGC AGTCCACTCC TAGGTAGTAA GTTTCATCAC 
CAGATCACAG ACCGCCCAAC CAGCCCTTTT AGACAGGTGG TAGGACCAAC ACGGAGTCTG 
CCACCAGAGA GTCCAAGACT TGGGCGGAGG AACTTTGAAA GCATGAGGGA CCTGCCTCCA 
CTTAGCCCTT CTCTGCCCCG AAGAAGTTCA TCTAGCTCCA TTAAACCTGC TCCAGACAGC 
CCATCTCGAG TTCCAGACAG CCCCAGCACT TTAGGGCGAC GTCGGGTGGG AAGTGCTAGC 
TCTCCTGGCA CAGAAAATAA TGCAAGTACA AGGCAGATAA GGGAGCGCAG CCCATCTCCC 
TCCTCTCTTC AAGAGACCTC TCGTCGACTA ACTCCAGCTT ATAGCCTGGG ATCGCTTGGG 
ACCCCTTCAC AAAGCCCCAG GGCACAAAGA AAAGGGCCTG TGCTACGGGA GCGCAAGAAC 
AGCATCAGTG AAATTTCTGG CAACCAAGAG GAGCTAATGG ACTACCACAG AAGGCAGAGA 
GAAGAAAGAC TTAGAGAGCA GGAAATGGAA AGGCTGGAGC GGCAGAGACT GGAAACCATT 
TTAAACTTGT GTGCCGAATA CAGTAAAACA GATGATACAG CTCCCCCAGC TCGCCTAGAT 
GAAATAAGGA GCTTCCCCAC TTTGGGGGGT ACTGAGGAAA CCACTGCATC CCTCTTCACT 
CGTAGACCTT CTTGGGAGAG CACCAGAAGT ACCAACCTGG AGGGAACAGG CAATAGGGTG 
AGAGCACAGG GAGAACACAA TGAGGAAGAG ACTCAGAGAG AAGAGAACAG CAGTACAGAG 
AGTACCCAAC ATGAGGAAGA TGCTGTAAGC CCAACTGGGG CTGCAGAATT ACTAGCCCTG 
GAGGAGCAAC GCAGGCACAC CCTGGGGCAT GTGGATCAGC TAAAGAATAG AGTGACTGAA 
TTGGAGGGTC AGTTGCAGGA AAGTGCAAGG GAGGCAGAGA TGGAACGTGC CCTTTTGCAA 
GGGGAACGGG AGGCTGAGCT GTCCCAGTTG CAGCATGAGC AAAGAATGGT CCAGCAGCTG 
CAGGACAGCC TGACCTCTTT AGAAAACAAC TTGCAGAAGG AGAAAGACAA GGCACGTGAG 
AAACTGGAGC AGGAGAGGAT GGAGCTGCAG AGTATTCAGC TTTCTCACCA CGACCTGCAG 
AGGCAGCTCC ATAACTGCCC TGAGGCTCTG CGGGAGCAGG TGCAGTGCCA GCTGACACGG 
GAGTCTGAAC TCCTGGAGAC GAAGATGAAG CTGTTTGAGG ATCTGGAGTT CCAGCAGCTA 
GAAAAGGAAA GCCGCCTGGA AGAAGAAAGA GAGACCACAA GTCAACAACT TTTACAGAGC 
AAGGGGGAAT GCCAGCGAAG CATTGCACGC AGGAAGGAAC GCCTGGCTGC TTTGGAGATG 
CAAGCCAATC AGATTCGCCT CCAGGCAGCA CAGGATGCAG ACCATCTTGG CCAGGAAAGA 
AGCGCCATAC TTTTACAGCT ACACAAGGAG AAAGAGAAGC TGGTTCAGTT GGAGAGAAGA 
TACCAGGCAC TAACAGGACG ACGGGGATTC TCAACTGGAT CAGCTGCTCT CCGAGAGTAC 
ATCACTTTAG ATCAGCTGCA GGATATTCTG GAAAGCATGC CCACAGGTTC TGCTCCAACT 
GCTGTGGCAA TGGCTGGCAC TGATATCAAC TCTGCCTCTT GTGCTCCTTC ACCTGACGAC 
CCTCCTCCTG TACATCTCTC GTCTTCATCT TCTTCCTCTC CCTCCATTTC TCTGGAGGTG 
AATTCCCCAA CAACAGAAGG TCACTCTGCC CCCCTAGCAC CTGATTTGGA TGCCTATTAC 
CAAGATTTAG TAACTGCACC TTCTGCCTCT TCTTCTCCAT CTTCTTCCCC TCCTCCACTC 
CCTGCTAAAT CCCACTCATC ACGTAAGGCT TTGCAGATTT CTCGCTCTCG GACAGAAATT 
GAGGGTGGAG GCACTCTTCC ACGGATGAAA ACAGTCTCCC CCGCTTCTTC TCACTTTTCA 
GTGGCCACTC TGGGCCGTAG TGTTTCCCCA AAGAATGCCC ATCTGACTCA GAATGGCACA 
GGCAGCCTCT CCAGGGGATT GGCAGTAACC ATGCAAGACA TTCAAAGCAA GAGACAGCTG 
GCACTGCAAC AAAAAGGTCA ACAAGTGATA GAGGAGCAGC GCAGACGTCT TGCAGAGTTG 
AAACAGAGAG CTGCAGTTGA GGCACAATCT CAGTGGGAAT CTCTCCATGG AGCCCCTAAT 
CAAGGGTACA CGCCTATAAT TCACCACTCC ATTCTACACC ATCAGCGGCC TCTCCATTCT 
CGTGAAGATG ACCCTACCCA TGGATTTGAC ACTCTCAGTC TGGAGAGTTC TGATAGTTTG 
GACACTAGTG TTTCTACAGG AAACTCTGCA TGTTCCCCTG ATAACATGTC AAGTGCTAGT 
GGGTTAGACA TGCTAAAGAT AGAGGAAATG GAAAGAATGC TTCTAGAAGC TCATGCAGAG 
CGTGCCAGGC TTGTAGAATC CAGGGAGCGT GAGGTGGAGG CACGCAGAAA AGCACTAGAA 
GATGAGAGGC GAAGGAGAGA GGAGCTGGAA AGGCGTCTTC AAGATGAGAC AGCTCACAGG 
CAAAGACTAG TAGAGAAGGA GGTCAAGATG AGGGAGAAGC ATTTTTCACA GGCTCGTCCT 
CTTACACGCT ATCTGCCAAT CCGGAAGGAA GACTTTGATC TCCGAGCCCA TGTTGAGTCC 
TCAGGCCATG CAGTAGATGC CTGCCCTCAC ATCATTTTAA GTGAGAAGAT GTGTCGTGGC 
TTCCTGACAA AAATGGGTGG AAAGATAAAA TCATGGAAGA AGCGCTGGTT TGTTTTTGAT 
CGCCTGAAAC GGACCCTGTC CTATTATGTA GATAAACATG AGGCAAAGCT GAAAGGAGTT 
ATATATTTCC AGGCCATAGA AGAGGTTTAT TATGACCACC TCAGGAGTGC AGCCAAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760733.1
CDS	264..4481
Translation	MLPLLPLFPSPHPAQITEKGRADYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_031904873.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904873.1

ORF Insert Sequence:

ATGCTTCCTC TCCTCCCTCT CTTCCCCTCG CCACATCCTG CCCAAATTAC AGAAAAAGGC 
AGAGCAGATT ATTTGGAAAC AGAATTCCCT GCTCAGACAG AAATAGAGGA CACAATGAAG 
GCACTGAGTC ATGATGTGAG CAGCCCCTGC AGAAAAACAC ACACAGTTAT TCAGAGCACA 
CCATTGGATC TCATAGATAC AGGAAAAAGT CTTAGGGTGC AGACAGAAAA ACCTCATCTT 
GTCAGCCTGG GAAGTGGACG ACTCAGCACT GCAATCACCC TACTACCACT GGAAGAAGGA 
AGGACAACCC TGGGTCATGC ATCTAAGGAC ATTCCCCTGC AGGGACCTGG CATTGCTGCA 
GAACATTGCT ACATTGAAAA CATCAGAGGA AACCTCACCC TATACCCATG TGGAAATCCT 
TGTGCCATCG ATGGACTGCT CATCACCCAA CCGACACGTC TCACTCAAGG GTGTATGGTC 
TGTCTGGGTC AGTCCACCTT CCTGAGATTT AACCACCCAG CTGAAGCTCA CTGGATGAAA 
ACCATGATTC CTTCCACTGG GAGAAGCCCT GCGCCAGGCT TGTACCCATC AGATTCTTCT 
TCCTCCTTGC TAAATGGTTC TCGTGATGCT GCATACCCGA GAACTGTACC TTCCCATACC 
TCACTAGTAA GCTCCATTGA AAAAAATCTT CAGGACATCA TGGACTCGTT GGTTTTAGAA 
GGGCCTGCAC CACTGAGCAG GAAGCTTTCC CAGACCTCAC TTAACCATAT CACCCCTATG 
ACCAATGGAG AGAACAGACG GTACTTGCTT TCATCTCCAA CAAGTCCACC TTACTCAAAC 
TCAGGTTATG ACAATACCTC TCCACTGTCT TCTCCTGGAA GTGGAAGTCT CACTAGCCCC 
TCTCCGGGTC AAGATGCAGG AACGCCAACA GCACCCCCTG TGGTGCCACT TCGAACTTCT 
AGCTTTAGCC ATTCCTTGCC ACGACCAGTT CCTACCTATG GAGGAAGCAA CCTTGACTTG 
CGTTCTAATC GCAACACAGG TGGTGGGTCT CTGCTTAGTC CTTCAAGCCC AGCAACAGGA 
AGCAGGATGG GGGGACTAAG TGTGCCAAGC AGTCCACTCC TAGGTAGTAA GTTTCATCAC 
CAGATCACAG ACCGCCCAAC CAGCCCTTTT AGACAGGTGG TAGGACCAAC ACGGAGTCTG 
CCACCAGAGA GTCCAAGACT TGGGCGGAGG AACTTTGAAA GCATGAGGGA CCTGCCTCCA 
CTTAGCCCTT CTCTGCCCCG AAGAAGTTCA TCTAGCTCCA TTAAACCTGC TCCAGACAGC 
CCATCTCGAG TTCCAGACAG CCCCAGCACT TTAGGGCGAC GTCGGGTGGG AAGTGCTAGC 
TCTCCTGGCA CAGAAAATAA TGCAAGTACA AGGCAGATAA GGGAGCGCAG CCCATCTCCC 
TCCTCTCTTC AAGAGACCTC TCGTCGACTA ACTCCAGCTT ATAGCCTGGG ATCGCTTGGG 
ACCCCTTCAC AAAGCCCCAG GGCACAAAGA AAAGGGCCTG TGCTACGGGA GCGCAAGAAC 
AGCATCAGTG AAATTTCTGG CAACCAAGAG GAGCTAATGG ACTACCACAG AAGGCAGAGA 
GAAGAAAGAC TTAGAGAGCA GGAAATGGAA AGGCTGGAGC GGCAGAGACT GGAAACCATT 
TTAAACTTGT GTGCCGAATA CAGTAAAACA GATGATACAG CTCCCCCAGC TCGCCTAGAT 
GAAATAAGGA GCTTCCCCAC TTTGGGGGGT ACTGAGGAAA CCACTGCATC CCTCTTCACT 
CGTAGACCTT CTTGGGAGAG CACCAGAAGT ACCAACCTGG AGGGAACAGG CAATAGGGTG 
AGAGCACAGG GAGAACACAA TGAGGAAGAG ACTCAGAGAG AAGAGAACAG CAGTACAGAG 
AGTACCCAAC ATGAGGAAGA TGCTGTAAGC CCAACTGGGG CTGCAGAATT ACTAGCCCTG 
GAGGAGCAAC GCAGGCACAC CCTGGGGCAT GTGGATCAGC TAAAGAATAG AGTGACTGAA 
TTGGAGGGTC AGTTGCAGGA AAGTGCAAGG GAGGCAGAGA TGGAACGTGC CCTTTTGCAA 
GGGGAACGGG AGGCTGAGCT GTCCCAGTTG CAGCATGAGC AAAGAATGGT CCAGCAGCTG 
CAGGACAGCC TGACCTCTTT AGAAAACAAC TTGCAGAAGG AGAAAGACAA GGCACGTGAG 
AAACTGGAGC AGGAGAGGAT GGAGCTGCAG AGTATTCAGC TTTCTCACCA CGACCTGCAG 
AGGCAGCTCC ATAACTGCCC TGAGGCTCTG CGGGAGCAGG TGCAGTGCCA GCTGACACGG 
GAGTCTGAAC TCCTGGAGAC GAAGATGAAG CTGTTTGAGG ATCTGGAGTT CCAGCAGCTA 
GAAAAGGAAA GCCGCCTGGA AGAAGAAAGA GAGACCACAA GTCAACAACT TTTACAGAGC 
AAGGGGGAAT GCCAGCGAAG CATTGCACGC AGGAAGGAAC GCCTGGCTGC TTTGGAGATG 
CAAGCCAATC AGATTCGCCT CCAGGCAGCA CAGGATGCAG ACCATCTTGG CCAGGAAAGA 
AGCGCCATAC TTTTACAGCT ACACAAGGAG AAAGAGAAGC TGGTTCAGTT GGAGAGAAGA 
TACCAGGCAC TAACAGGACG ACGGGGATTC TCAACTGGAT CAGCTGCTCT CCGAGAGTAC 
ATCACTTTAG ATCAGCTGCA GGATATTCTG GAAAGCATGC CCACAGGTTC TGCTCCAACT 
GCTGTGGCAA TGGCTGGCAC TGATATCAAC TCTGCCTCTT GTGCTCCTTC ACCTGACGAC 
CCTCCTCCTG TACATCTCTC GTCTTCATCT TCTTCCTCTC CCTCCATTTC TCTGGAGGTG 
AATTCCCCAA CAACAGAAGG TCACTCTGCC CCCCTAGCAC CTGATTTGGA TGCCTATTAC 
CAAGATTTAG TAACTGCACC TTCTGCCTCT TCTTCTCCAT CTTCTTCCCC TCCTCCACTC 
CCTGCTAAAT CCCACTCATC ACGTAAGGCT TTGCAGATTT CTCGCTCTCG GACAGAAATT 
GAGGGTGGAG GCACTCTTCC ACGGATGAAA ACAGTCTCCC CCGCTTCTTC TCACTTTTCA 
GTGGCCACTC TGGGCCGTAG TGTTTCCCCA AAGAATGCCC ATCTGACTCA GAATGGCACA 
GGCAGCCTCT CCAGGGGATT GGCAGTAACC ATGCAAGACA TTCAAAGCAA GAGACAGCTG 
GCACTGCAAC AAAAAGGTCA ACAAGTGATA GAGGAGCAGC GCAGACGTCT TGCAGAGTTG 
AAACAGAGAG CTGCAGTTGA GGCACAATCT CAGTGGGAAT CTCTCCATGG AGCCCCTAAT 
CAAGGGTACA CGCCTATAAT TCACCACTCC ATTCTACACC ATCAGCGGCC TCTCCATTCT 
CGTGAAGATG ACCCTACCCA TGGATTTGAC ACTCTCAGTC TGGAGAGTTC TGATAGTTTG 
GACACTAGTG TTTCTACAGG AAACTCTGCA TGTTCCCCTG ATAACATGTC AAGTGCTAGT 
GGGTTAGACA TGCTAAAGAT AGAGGAAATG GAAAGAATGC TTCTAGAAGC TCATGCAGAG 
CGTGCCAGGC TTGTAGAATC CAGGGAGCGT GAGGTGGAGG CACGCAGAAA AGCACTAGAA 
GATGAGAGGC GAAGGAGAGA GGAGCTGGAA AGGCGTCTTC AAGATGAGAC AGCTCACAGG 
CAAAGACTAG TAGAGAAGGA GGTCAAGATG AGGGAGAAGC ATTTTTCACA GGCTCGTCCT 
CTTACACGCT ATCTGCCAAT CCGGAAGGAA GACTTTGATC TCCGAGCCCA TGTTGAGTCC 
TCAGGCCATG CAGTAGATGC CTGCCCTCAC ATCATTTTAA GTGAGAAGAT GTGTCGTGGC 
TTCCTGACAA AAATGGGTGG AAAGATAAAA TCATGGAAGA AGCGCTGGTT TGTTTTTGAT 
CGCCTGAAAC GGACCCTGTC CTATTATGTA GATAAACATG AGGCAAAGCT GAAAGGAGTT 
ATATATTTCC AGGCCATAGA AGAGGTTTAT TATGACCACC TCAGGAGTGC AGCCAAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966183.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821637.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X18, mRNA.

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CloneID	OXa32420
Clone ID Related Accession (Same CDS sequence)	XM_012966183.2 , XM_012966183.3
Accession Version	XM_012966183.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA 
CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT 
GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG 
GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGGTCAA 
CAAGTGATAG AGGAGCAGCG CAGACGTCTT GCAGAGTTGA AACAGAGAGC TGCAGTTGAG 
GCACAATCTC AGTGGGAATC TCTCCATGGA GCCCCTAATC AAGGGTACAC GCCTATAATT 
CACCACTCCA TTCTACACCA TCAGCGGCCT CTCCATTCTC GTGAAGATGA CCCTACCCAT 
GGATTTGACA CTCTCAGTCT GGAGAGTTCT GATAGTTTGG ACACTAGTGT TTCTACAGGA 
AACTCTGCAT GTTCCCCTGA TAACATGTCA AGTGCTAGTG GGTTAGACAT GCTAAAGATA 
GAGGAAATGG AAAGAATGCT TCTAGAAGCT CATGCAGAGC GTGCCAGGCT TGTAGAATCC 
AGGGAGCGTG AGGTGGAGGC ACGCAGAAAA GCACTAGAAG ATGAGAGGCG AAGGAGAGAG 
GAGCTGGAAA GGCGTCTTCA AGATGAGACA GCTCACAGGC AAAGACTAGT AGAGAAGGAG 
GTCAAGATGA GGGAGAAGCA TTTTTCACAG GCTCGTCCTC TTACACGCTA TCTGCCAATC 
CGGAAGGAAG ACTTTGATCT CCGAGCCCAT GTTGAGTCCT CAGGCCATGC AGTAGATGCC 
TGCCCTCACA TCATTTTAAG TGAGAAGATG TGTCGTGGCT TCCTGACAAA AATGGGTGGA 
AAGATAAAAT CATGGAAGAA GCGCTGGTTT GTTTTTGATC GCCTGAAACG GACCCTGTCC 
TATTATGTAG ATAAACATGA GGCAAAGCTG AAAGGAGTTA TATATTTCCA GGCCATAGAA 
GAGGTTTATT ATGACCACCT CAGGAGTGCA GCCAAGAAAG GACTTTTTAA CCTCAGCCTG 
GCCACTCAGA TATCCGATTT TCTTTCTGTG CCCCTGTGGA AGGAGAGTCC TAACCCAGCT 
CTAACATTCT GTGTCAAGAC TCACGATCGC CTGTATTACA TGGTAGCTCC TTCCCCAGAG 
GCTATGCGGA TATGGATGGA TGTCATTGTA ACTGGGGCAG AGGGTTACAC TCAGTTCATG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821637.1
CDS	58..4023
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966183.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966183.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA 
CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT 
GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG 
GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGGTCAA 
CAAGTGATAG AGGAGCAGCG CAGACGTCTT GCAGAGTTGA AACAGAGAGC TGCAGTTGAG 
GCACAATCTC AGTGGGAATC TCTCCATGGA GCCCCTAATC AAGGGTACAC GCCTATAATT 
CACCACTCCA TTCTACACCA TCAGCGGCCT CTCCATTCTC GTGAAGATGA CCCTACCCAT 
GGATTTGACA CTCTCAGTCT GGAGAGTTCT GATAGTTTGG ACACTAGTGT TTCTACAGGA 
AACTCTGCAT GTTCCCCTGA TAACATGTCA AGTGCTAGTG GGTTAGACAT GCTAAAGATA 
GAGGAAATGG AAAGAATGCT TCTAGAAGCT CATGCAGAGC GTGCCAGGCT TGTAGAATCC 
AGGGAGCGTG AGGTGGAGGC ACGCAGAAAA GCACTAGAAG ATGAGAGGCG AAGGAGAGAG 
GAGCTGGAAA GGCGTCTTCA AGATGAGACA GCTCACAGGC AAAGACTAGT AGAGAAGGAG 
GTCAAGATGA GGGAGAAGCA TTTTTCACAG GCTCGTCCTC TTACACGCTA TCTGCCAATC 
CGGAAGGAAG ACTTTGATCT CCGAGCCCAT GTTGAGTCCT CAGGCCATGC AGTAGATGCC 
TGCCCTCACA TCATTTTAAG TGAGAAGATG TGTCGTGGCT TCCTGACAAA AATGGGTGGA 
AAGATAAAAT CATGGAAGAA GCGCTGGTTT GTTTTTGATC GCCTGAAACG GACCCTGTCC 
TATTATGTAG ATAAACATGA GGCAAAGCTG AAAGGAGTTA TATATTTCCA GGCCATAGAA 
GAGGTTTATT ATGACCACCT CAGGAGTGCA GCCAAGAAAG GACTTTTTAA CCTCAGCCTG 
GCCACTCAGA TATCCGATTT TCTTTCTGTG CCCCTGTGGA AGGAGAGTCC TAACCCAGCT 
CTAACATTCT GTGTCAAGAC TCACGATCGC CTGTATTACA TGGTAGCTCC TTCCCCAGAG 
GCTATGCGGA TATGGATGGA TGTCATTGTA ACTGGGGCAG AGGGTTACAC TCAGTTCATG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966184.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821638.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OXa32421
Clone ID Related Accession (Same CDS sequence)	XM_012966184.2 , XM_012966184.3
Accession Version	XM_012966184.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821638.1
CDS	58..3894
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966184.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966184.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966185.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821639.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OXa32422
Clone ID Related Accession (Same CDS sequence)	XM_012966185.2 , XM_012966185.3
Accession Version	XM_012966185.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966185.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG 
ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG 
GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821639.1
CDS	56..3823
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966185.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966185.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG 
ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG 
GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966165.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821619.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X1, mRNA.

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CloneID	OXa32404
Clone ID Related Accession (Same CDS sequence)	XM_012966165.3 , XM_012966165.2
Accession Version	XM_012966165.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT 
CAGATATCCG ATTTTCTTTC TGTGCCCCTG TGGAAGGAGA GTCCTAACCC AGCTCTAACA 
TTCTGTGTCA AGACTCACGA TCGCCTGTAT TACATGGTAG CTCCTTCCCC AGAGGCTATG 
CGGATATGGA TGGATGTCAT TGTAACTGGG GCAGAGGGTT ACACTCAGTT CATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821619.1
CDS	35..4594
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966165.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966165.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT 
CAGATATCCG ATTTTCTTTC TGTGCCCCTG TGGAAGGAGA GTCCTAACCC AGCTCTAACA 
TTCTGTGTCA AGACTCACGA TCGCCTGTAT TACATGGTAG CTCCTTCCCC AGAGGCTATG 
CGGATATGGA TGGATGTCAT TGTAACTGGG GCAGAGGGTT ACACTCAGTT CATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966167.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821621.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X2, mRNA.

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CloneID	OXa32405
Clone ID Related Accession (Same CDS sequence)	XM_012966167.2 , XM_012966167.3
Accession Version	XM_012966167.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966167.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA 
GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG 
AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA 
CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA 
ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA 
GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT 
CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG 
TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG 
GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA 
CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG 
GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT 
CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT 
CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT 
GCTCTCCGAG AGGATGCTCT TCAAATCTAT GAGACTAGAG ACTGCTTACT CGCTCAAGCT 
GATGTTTCTT GCACACCTGC TATCCCTTCT GCCTGCTCTC ACTCCCCTTC CCGGCAGCAA 
GAGGAATACC TGTGCCTCTC TGATGTTCTC CGCTTGTACA GCAGCTCGCC TCTTGTTCCT 
GCTTTTCCCC TTCTCTCTGC TGCAGAGCCC ACCGCCGCCC TTGAGTACAT CACTTTAGAT 
CAGCTGCAGG ATATTCTGGA AAGCATGCCC ACAGGTTCTG CTCCAACTGC TGTGGCAATG 
GCTGGCACTG ATATCAACTC TGCCTCTTGT GCTCCTTCAC CTGACGACCC TCCTCCTGTA 
CATCTCTCGT CTTCATCTTC TTCCTCTCCC TCCATTTCTC TGGAGGTGAA TTCCCCAACA 
ACAGAAGGTC ACTCTGCCCC CCTAGCACCT GATTTGGATG CCTATTACCA AGATTTAGTA 
ACTGCACCTT CTGCCTCTTC TTCTCCATCT TCTTCCCCTC CTCCACTCCC TGCTAAATCC 
CACTCATCAC GTAAGGCTTT GCAGATTTCT CGCTCTCGGA CAGAAATTGA GGGTGGAGGC 
ACTCTTCCAC GGATGAAAAC AGTCTCCCCC GCTTCTTCTC ACTTTTCAGT GGCCACTCTG 
GGCCGTAGTG TTTCCCCAAA GAATGCCCAT CTGACTCAGA ATGGCACAGG CAGCCTCTCC 
AGGGGATTGG CAGTAACCAT GCAAGACATT CAAAGCAAGA GACAGCTGGC ACTGCAACAA 
AAAGACAGTC TGAGCACAGA AGATTCTCTT CCATCTGAAG TTGAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821621.1
CDS	35..4591
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966167.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966167.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA 
GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG 
AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA 
CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA 
ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA 
GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT 
CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG 
TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG 
GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA 
CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG 
GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT 
CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT 
CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT 
GCTCTCCGAG AGGATGCTCT TCAAATCTAT GAGACTAGAG ACTGCTTACT CGCTCAAGCT 
GATGTTTCTT GCACACCTGC TATCCCTTCT GCCTGCTCTC ACTCCCCTTC CCGGCAGCAA 
GAGGAATACC TGTGCCTCTC TGATGTTCTC CGCTTGTACA GCAGCTCGCC TCTTGTTCCT 
GCTTTTCCCC TTCTCTCTGC TGCAGAGCCC ACCGCCGCCC TTGAGTACAT CACTTTAGAT 
CAGCTGCAGG ATATTCTGGA AAGCATGCCC ACAGGTTCTG CTCCAACTGC TGTGGCAATG 
GCTGGCACTG ATATCAACTC TGCCTCTTGT GCTCCTTCAC CTGACGACCC TCCTCCTGTA 
CATCTCTCGT CTTCATCTTC TTCCTCTCCC TCCATTTCTC TGGAGGTGAA TTCCCCAACA 
ACAGAAGGTC ACTCTGCCCC CCTAGCACCT GATTTGGATG CCTATTACCA AGATTTAGTA 
ACTGCACCTT CTGCCTCTTC TTCTCCATCT TCTTCCCCTC CTCCACTCCC TGCTAAATCC 
CACTCATCAC GTAAGGCTTT GCAGATTTCT CGCTCTCGGA CAGAAATTGA GGGTGGAGGC 
ACTCTTCCAC GGATGAAAAC AGTCTCCCCC GCTTCTTCTC ACTTTTCAGT GGCCACTCTG 
GGCCGTAGTG TTTCCCCAAA GAATGCCCAT CTGACTCAGA ATGGCACAGG CAGCCTCTCC 
AGGGGATTGG CAGTAACCAT GCAAGACATT CAAAGCAAGA GACAGCTGGC ACTGCAACAA 
AAAGACAGTC TGAGCACAGA AGATTCTCTT CCATCTGAAG TTGAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966168.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821622.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa32406
Clone ID Related Accession (Same CDS sequence)	XM_012966168.2 , XM_012966168.3
Accession Version	XM_012966168.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966168.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGAG 
TCTGAACTCC TGGAGACGAA GATGAAGCTG TTTGAGGATC TGGAGTTCCA GCAGCTAGAA 
AAGGAAAGCC GCCTGGAAGA AGAAAGAGAG ACCACAAGTC AACAACTTTT ACAGAGCAAG 
GGGGAATGCC AGCGAAGCAT TGCACGCAGG AAGGAACGCC TGGCTGCTTT GGAGATGCAA 
GCCAATCAGA TTCGCCTCCA GGCAGCACAG GATGCAGACC ATCTTGGCCA GGAAAGAAGC 
GCCATACTTT TACAGCTACA CAAGGAGAAA GAGAAGCTGG TTCAGTTGGA GAGAAGATAC 
CAGGCACTAA CAGGACGACG GGGATTCTCA ACTGGATCAG CTGCTCTCCG AGAGGATGCT 
CTTCAAATCT ATGAGACTAG AGACTGCTTA CTCGCTCAAG CTGATGTTTC TTGCACACCT 
GCTATCCCTT CTGCCTGCTC TCACTCCCCT TCCCGGCAGC AAGAGGAATA CCTGTGCCTC 
TCTGATGTTC TCCGCTTGTA CAGCAGCTCG CCTCTTGTTC CTGCTTTTCC CCTTCTCTCT 
GCTGCAGAGC CCACCGCCGC CCTTGAGTAC ATCACTTTAG ATCAGCTGCA GGATATTCTG 
GAAAGCATGC CCACAGGTTC TGCTCCAACT GCTGTGGCAA TGGCTGGCAC TGATATCAAC 
TCTGCCTCTT GTGCTCCTTC ACCTGACGAC CCTCCTCCTG TACATCTCTC GTCTTCATCT 
TCTTCCTCTC CCTCCATTTC TCTGGAGGTG AATTCCCCAA CAACAGAAGG TCACTCTGCC 
CCCCTAGCAC CTGATTTGGA TGCCTATTAC CAAGATTTAG TAACTGCACC TTCTGCCTCT 
TCTTCTCCAT CTTCTTCCCC TCCTCCACTC CCTGCTAAAT CCCACTCATC ACGTAAGGCT 
TTGCAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA 
GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT 
CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT 
GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG 
CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT 
TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG 
TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA 
GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA 
AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG 
ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA 
CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC 
CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG 
ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG 
TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG 
CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT 
GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT 
GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT 
CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT 
GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821622.1
CDS	35..4573
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966168.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966168.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGAG 
TCTGAACTCC TGGAGACGAA GATGAAGCTG TTTGAGGATC TGGAGTTCCA GCAGCTAGAA 
AAGGAAAGCC GCCTGGAAGA AGAAAGAGAG ACCACAAGTC AACAACTTTT ACAGAGCAAG 
GGGGAATGCC AGCGAAGCAT TGCACGCAGG AAGGAACGCC TGGCTGCTTT GGAGATGCAA 
GCCAATCAGA TTCGCCTCCA GGCAGCACAG GATGCAGACC ATCTTGGCCA GGAAAGAAGC 
GCCATACTTT TACAGCTACA CAAGGAGAAA GAGAAGCTGG TTCAGTTGGA GAGAAGATAC 
CAGGCACTAA CAGGACGACG GGGATTCTCA ACTGGATCAG CTGCTCTCCG AGAGGATGCT 
CTTCAAATCT ATGAGACTAG AGACTGCTTA CTCGCTCAAG CTGATGTTTC TTGCACACCT 
GCTATCCCTT CTGCCTGCTC TCACTCCCCT TCCCGGCAGC AAGAGGAATA CCTGTGCCTC 
TCTGATGTTC TCCGCTTGTA CAGCAGCTCG CCTCTTGTTC CTGCTTTTCC CCTTCTCTCT 
GCTGCAGAGC CCACCGCCGC CCTTGAGTAC ATCACTTTAG ATCAGCTGCA GGATATTCTG 
GAAAGCATGC CCACAGGTTC TGCTCCAACT GCTGTGGCAA TGGCTGGCAC TGATATCAAC 
TCTGCCTCTT GTGCTCCTTC ACCTGACGAC CCTCCTCCTG TACATCTCTC GTCTTCATCT 
TCTTCCTCTC CCTCCATTTC TCTGGAGGTG AATTCCCCAA CAACAGAAGG TCACTCTGCC 
CCCCTAGCAC CTGATTTGGA TGCCTATTAC CAAGATTTAG TAACTGCACC TTCTGCCTCT 
TCTTCTCCAT CTTCTTCCCC TCCTCCACTC CCTGCTAAAT CCCACTCATC ACGTAAGGCT 
TTGCAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA 
GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT 
CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT 
GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG 
CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT 
TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG 
TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA 
GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA 
AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG 
ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA 
CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC 
CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG 
ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG 
TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG 
CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT 
GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT 
GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT 
CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT 
GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966169.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821623.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X5, mRNA.

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CloneID	OXa32407
Clone ID Related Accession (Same CDS sequence)	XM_012966169.3 , XM_012966169.2
Accession Version	XM_012966169.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821623.1
CDS	35..4555
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966169.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966169.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966170.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821624.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X6, mRNA.

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CloneID	OXa32408
Clone ID Related Accession (Same CDS sequence)	XM_012966170.3 , XM_012966170.2
Accession Version	XM_012966170.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966170.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT 
AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821624.1
CDS	35..4552
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966170.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966170.3

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT 
AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_031904869.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760729.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X3, mRNA.

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CloneID	OXa61272
Clone ID Related Accession (Same CDS sequence)	XM_031904869.1
Accession Version	XM_031904869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760729.1
CDS	35..4591
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_031904869.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904869.1

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( NM_001130332.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123804.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), mRNA.

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CloneID	OXa02934
Clone ID Related Accession (Same CDS sequence)	NM_001130332.1
Accession Version	NM_001130332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167599.1. ##Evidence-Data-START## Transcript exon combination :: BC167599.1 [ECO:0000332] ##Evidence-Data-END##

ATGAAGGCAC TGAGTCATGA TGTGAGCAGC CCCTGCAGAA AAACACACAC AGTTATTCAG 
AGCACACCAT TGGATCTCAT AGATACAGGA AAAAGTCTTA GGGTGCAGAC AGAAAAACCT 
CATCTTGTCA GCCTGGGAAG TGGACGACTC AGCACTGCAA TCACCCTACT ACCACTGGAA 
GAAGGAAGGA CAACCCTGGG TCATGCATCT AAGGACATTC CCCTGCAGGG ACCTGGCATT 
GCTGCAGAAC ATTGCTACAT TGAAAACATC AGAGGAAACC TCACCCTATA CCCATGTGGA 
AATCCTTGTG CCATCGATGG ACTGCTCATC ACCCAACCGA CACGTCTCAC TCAAGGGTGT 
ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT 
TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC 
CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT 
TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC 
CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC 
TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT 
AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA 
ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT 
GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA 
ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT 
CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG 
AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG 
CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA 
GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT 
GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA 
TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG 
CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC 
AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG 
CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA 
ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC 
CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC 
TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT 
AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT 
ACAGAGAGTA CCCAACATGA GGAAGATGCT GTAAGCCCAA CTGGGGCTGC AGAATTACTA 
GCCCTGGAGG AGCAACGCAG GCACACCCTG GGGCATGTGG ATCAGCTAAA GAATAGAGTG 
ACTGAATTGG AGGGTCAGTT GCAGGAAAGT GCAAGGGAGG CAGAGATGGA ACGTGCCCTT 
TTGCAAGGGG AACGGGAGGC TGAGCTGTCC CAGTTGCAGC ATGAGCAAAG AATGGTCCAG 
CAGCTGCAGG ACAGCCTGAC CTCTTTAGAA AACAACTTGC AGAAGGAGAA AGACAAGGCA 
CGTGAGAAAC TGGAGCAGGA GAGGATGGAG CTGCAGAGTA TTCAGCTTTC TCACCACGAC 
CTGCAGAGGC AGCTCCATAA CTGCCCTGAG GCTCTGCGGG AGCAGGTGCA GTGCCAGCTG 
ACACGGGAGT CTGAACTCCT GGAGACGAAG ATGAAGCTGT TTGAGGATCT GGAGTTCCAG 
CAGCTAGAAA AGGAAAGCCG CCTGGAAGAA GAAAGAGAGA CCACAAGTCA ACAACTTTTA 
CAGAGCAAGG GGGAATGCCA GCGAAGCATT GCACGCAGGA AGGAACGCCT GGCTGCTTTG 
GAGATGCAAG CCAATCAGAT TCGCCTCCAG GCAGCACAGG ATGCAGACCA TCTTGGCCAG 
GAAAGAAGCG CCATACTTTT ACAGCTACAC AAGGAGAAAG AGAAGCTGGT TCAGTTGGAG 
AGAAGATACC AGGCACTAAC AGGACGACGG GGATTCTCAA CTGGATCAGC TGCTCTCCGA 
GAGTACATCA CTTTAGATCA GCTGCAGGAT ATTCTGGAAA GCATGCCCAC AGGTTCTGCT 
CCAACTGCTG TGGCAATGGC TGGCACTGAT ATCAACTCTG CCTCTTGTGC TCCTTCACCT 
GACGACCCTC CTCCTGTACA TCTCTCGTCT TCATCTTCTT CCTCTCCCTC CATTTCTCTG 
GAGGTGAATT CCCCAACAAC AGAAGGTCAC TCTGCCCCCC TAGCACCTGA TTTGGATGCC 
TATTACCAAG ATTTAGTAAC TGCACCTTCT GCCTCTTCTT CTCCATCTTC TTCCCCTCCT 
CCACTCCCTG CTAAATCCCA CTCATCACGT AAGGCTTTGC AGATTTCTCG CTCTCGGACA 
GAAATTGAGG GTGGAGGCAC TCTTCCACGG ATGAAAACAG TCTCCCCCGC TTCTTCTCAC 
TTTTCAGTGG CCACTCTGGG CCGTAGTGTT TCCCCAAAGA ATGCCCATCT GACTCAGAAT 
GGCACAGGCA GCCTCTCCAG GGGATTGGCA GTAACCATGC AAGACATTCA AAGCAAGAGA 
CAGCTGGCAC TGCAACAAAA AGGTCAACAA GTGATAGAGG AGCAGCGCAG ACGTCTTGCA 
GAGTTGAAAC AGAGAGCTGC AGTTGAGGCA CAATCTCAGT GGGAATCTCT CCATGGAGCC 
CCTAATCAAG GGTACACGCC TATAATTCAC CACTCCATTC TACACCATCA GCGGCCTCTC 
CATTCTCGTG AAGATGACCC TACCCATGGA TTTGACACTC TCAGTCTGGA GAGTTCTGAT 
AGTTTGGACA CTAGTGTTTC TACAGGAAAC TCTGCATGTT CCCCTGATAA CATGTCAAGT 
GCTAGTGGGT TAGACATGCT AAAGATAGAG GAAATGGAAA GAATGCTTCT AGAAGCTCAT 
GCAGAGCGTG CCAGGCTTGT AGAATCCAGG GAGCGTGAGG TGGAGGCACG CAGAAAAGCA 
CTAGAAGATG AGAGGCGAAG GAGAGAGGAG CTGGAAAGGC GTCTTCAAGA TGAGACAGCT 
CACAGGCAAA GACTAGTAGA GAAGGAGGTC AAGATGAGGG AGAAGCATTT TTCACAGGCT 
CGTCCTCTTA CACGCTATCT GCCAATCCGG AAGGAAGACT TTGATCTCCG AGCCCATGTT 
GAGTCCTCAG GCCATGCAGT AGATGCCTGC CCTCACATCA TTTTAAGTGA GAAGATGTGT 
CGTGGCTTCC TGACAAAAAT GGGTGGAAAG ATAAAATCAT GGAAGAAGCG CTGGTTTGTT 
TTTGATCGCC TGAAACGGAC CCTGTCCTAT TATGTAGATA AACATGAGGC AAAGCTGAAA 
GGAGTTATAT ATTTCCAGGC CATAGAAGAG GTTTATTATG ACCACCTCAG GAGTGCAGCC 
AAGAGTCCTA ACCCAGCTCT AACATTCTGT GTCAAGACTC ACGATCGCCT GTATTACATG 
GTAGCTCCTT CCCCAGAGGC TATGCGGATA TGGATGGATG TCATTGTAAC TGGGGCAGAG 
GGTTACACTC AGTTCATGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123804.1
CDS	378..4481
Translation	MKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	NM_001130332.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130332.1

ORF Insert Sequence:

ATGAAGGCAC TGAGTCATGA TGTGAGCAGC CCCTGCAGAA AAACACACAC AGTTATTCAG 
AGCACACCAT TGGATCTCAT AGATACAGGA AAAAGTCTTA GGGTGCAGAC AGAAAAACCT 
CATCTTGTCA GCCTGGGAAG TGGACGACTC AGCACTGCAA TCACCCTACT ACCACTGGAA 
GAAGGAAGGA CAACCCTGGG TCATGCATCT AAGGACATTC CCCTGCAGGG ACCTGGCATT 
GCTGCAGAAC ATTGCTACAT TGAAAACATC AGAGGAAACC TCACCCTATA CCCATGTGGA 
AATCCTTGTG CCATCGATGG ACTGCTCATC ACCCAACCGA CACGTCTCAC TCAAGGGTGT 
ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT 
TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC 
CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT 
TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC 
CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC 
TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT 
AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA 
ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT 
GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA 
ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT 
CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG 
AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG 
CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA 
GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT 
GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA 
TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG 
CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC 
AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG 
CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA 
ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC 
CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC 
TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT 
AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT 
ACAGAGAGTA CCCAACATGA GGAAGATGCT GTAAGCCCAA CTGGGGCTGC AGAATTACTA 
GCCCTGGAGG AGCAACGCAG GCACACCCTG GGGCATGTGG ATCAGCTAAA GAATAGAGTG 
ACTGAATTGG AGGGTCAGTT GCAGGAAAGT GCAAGGGAGG CAGAGATGGA ACGTGCCCTT 
TTGCAAGGGG AACGGGAGGC TGAGCTGTCC CAGTTGCAGC ATGAGCAAAG AATGGTCCAG 
CAGCTGCAGG ACAGCCTGAC CTCTTTAGAA AACAACTTGC AGAAGGAGAA AGACAAGGCA 
CGTGAGAAAC TGGAGCAGGA GAGGATGGAG CTGCAGAGTA TTCAGCTTTC TCACCACGAC 
CTGCAGAGGC AGCTCCATAA CTGCCCTGAG GCTCTGCGGG AGCAGGTGCA GTGCCAGCTG 
ACACGGGAGT CTGAACTCCT GGAGACGAAG ATGAAGCTGT TTGAGGATCT GGAGTTCCAG 
CAGCTAGAAA AGGAAAGCCG CCTGGAAGAA GAAAGAGAGA CCACAAGTCA ACAACTTTTA 
CAGAGCAAGG GGGAATGCCA GCGAAGCATT GCACGCAGGA AGGAACGCCT GGCTGCTTTG 
GAGATGCAAG CCAATCAGAT TCGCCTCCAG GCAGCACAGG ATGCAGACCA TCTTGGCCAG 
GAAAGAAGCG CCATACTTTT ACAGCTACAC AAGGAGAAAG AGAAGCTGGT TCAGTTGGAG 
AGAAGATACC AGGCACTAAC AGGACGACGG GGATTCTCAA CTGGATCAGC TGCTCTCCGA 
GAGTACATCA CTTTAGATCA GCTGCAGGAT ATTCTGGAAA GCATGCCCAC AGGTTCTGCT 
CCAACTGCTG TGGCAATGGC TGGCACTGAT ATCAACTCTG CCTCTTGTGC TCCTTCACCT 
GACGACCCTC CTCCTGTACA TCTCTCGTCT TCATCTTCTT CCTCTCCCTC CATTTCTCTG 
GAGGTGAATT CCCCAACAAC AGAAGGTCAC TCTGCCCCCC TAGCACCTGA TTTGGATGCC 
TATTACCAAG ATTTAGTAAC TGCACCTTCT GCCTCTTCTT CTCCATCTTC TTCCCCTCCT 
CCACTCCCTG CTAAATCCCA CTCATCACGT AAGGCTTTGC AGATTTCTCG CTCTCGGACA 
GAAATTGAGG GTGGAGGCAC TCTTCCACGG ATGAAAACAG TCTCCCCCGC TTCTTCTCAC 
TTTTCAGTGG CCACTCTGGG CCGTAGTGTT TCCCCAAAGA ATGCCCATCT GACTCAGAAT 
GGCACAGGCA GCCTCTCCAG GGGATTGGCA GTAACCATGC AAGACATTCA AAGCAAGAGA 
CAGCTGGCAC TGCAACAAAA AGGTCAACAA GTGATAGAGG AGCAGCGCAG ACGTCTTGCA 
GAGTTGAAAC AGAGAGCTGC AGTTGAGGCA CAATCTCAGT GGGAATCTCT CCATGGAGCC 
CCTAATCAAG GGTACACGCC TATAATTCAC CACTCCATTC TACACCATCA GCGGCCTCTC 
CATTCTCGTG AAGATGACCC TACCCATGGA TTTGACACTC TCAGTCTGGA GAGTTCTGAT 
AGTTTGGACA CTAGTGTTTC TACAGGAAAC TCTGCATGTT CCCCTGATAA CATGTCAAGT 
GCTAGTGGGT TAGACATGCT AAAGATAGAG GAAATGGAAA GAATGCTTCT AGAAGCTCAT 
GCAGAGCGTG CCAGGCTTGT AGAATCCAGG GAGCGTGAGG TGGAGGCACG CAGAAAAGCA 
CTAGAAGATG AGAGGCGAAG GAGAGAGGAG CTGGAAAGGC GTCTTCAAGA TGAGACAGCT 
CACAGGCAAA GACTAGTAGA GAAGGAGGTC AAGATGAGGG AGAAGCATTT TTCACAGGCT 
CGTCCTCTTA CACGCTATCT GCCAATCCGG AAGGAAGACT TTGATCTCCG AGCCCATGTT 
GAGTCCTCAG GCCATGCAGT AGATGCCTGC CCTCACATCA TTTTAAGTGA GAAGATGTGT 
CGTGGCTTCC TGACAAAAAT GGGTGGAAAG ATAAAATCAT GGAAGAAGCG CTGGTTTGTT 
TTTGATCGCC TGAAACGGAC CCTGTCCTAT TATGTAGATA AACATGAGGC AAAGCTGAAA 
GGAGTTATAT ATTTCCAGGC CATAGAAGAG GTTTATTATG ACCACCTCAG GAGTGCAGCC 
AAGAGTCCTA ACCCAGCTCT AACATTCTGT GTCAAGACTC ACGATCGCCT GTATTACATG 
GTAGCTCCTT CCCCAGAGGC TATGCGGATA TGGATGGATG TCATTGTAAC TGGGGCAGAG 
GGTTACACTC AGTTCATGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966165.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821619.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X1, mRNA.

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CloneID	OXa32404
Clone ID Related Accession (Same CDS sequence)	XM_012966165.3 , XM_012966165.2
Accession Version	XM_012966165.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT 
CAGATATCCG ATTTTCTTTC TGTGCCCCTG TGGAAGGAGA GTCCTAACCC AGCTCTAACA 
TTCTGTGTCA AGACTCACGA TCGCCTGTAT TACATGGTAG CTCCTTCCCC AGAGGCTATG 
CGGATATGGA TGGATGTCAT TGTAACTGGG GCAGAGGGTT ACACTCAGTT CATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821619.1
CDS	64..4623
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966165.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966165.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT 
CAGATATCCG ATTTTCTTTC TGTGCCCCTG TGGAAGGAGA GTCCTAACCC AGCTCTAACA 
TTCTGTGTCA AGACTCACGA TCGCCTGTAT TACATGGTAG CTCCTTCCCC AGAGGCTATG 
CGGATATGGA TGGATGTCAT TGTAACTGGG GCAGAGGGTT ACACTCAGTT CATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966167.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821621.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X2, mRNA.

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CloneID	OXa32405
Clone ID Related Accession (Same CDS sequence)	XM_012966167.2 , XM_012966167.3
Accession Version	XM_012966167.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA 
GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG 
AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA 
CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA 
ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA 
GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT 
CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG 
TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG 
GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA 
CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG 
GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT 
CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT 
CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT 
GCTCTCCGAG AGGATGCTCT TCAAATCTAT GAGACTAGAG ACTGCTTACT CGCTCAAGCT 
GATGTTTCTT GCACACCTGC TATCCCTTCT GCCTGCTCTC ACTCCCCTTC CCGGCAGCAA 
GAGGAATACC TGTGCCTCTC TGATGTTCTC CGCTTGTACA GCAGCTCGCC TCTTGTTCCT 
GCTTTTCCCC TTCTCTCTGC TGCAGAGCCC ACCGCCGCCC TTGAGTACAT CACTTTAGAT 
CAGCTGCAGG ATATTCTGGA AAGCATGCCC ACAGGTTCTG CTCCAACTGC TGTGGCAATG 
GCTGGCACTG ATATCAACTC TGCCTCTTGT GCTCCTTCAC CTGACGACCC TCCTCCTGTA 
CATCTCTCGT CTTCATCTTC TTCCTCTCCC TCCATTTCTC TGGAGGTGAA TTCCCCAACA 
ACAGAAGGTC ACTCTGCCCC CCTAGCACCT GATTTGGATG CCTATTACCA AGATTTAGTA 
ACTGCACCTT CTGCCTCTTC TTCTCCATCT TCTTCCCCTC CTCCACTCCC TGCTAAATCC 
CACTCATCAC GTAAGGCTTT GCAGATTTCT CGCTCTCGGA CAGAAATTGA GGGTGGAGGC 
ACTCTTCCAC GGATGAAAAC AGTCTCCCCC GCTTCTTCTC ACTTTTCAGT GGCCACTCTG 
GGCCGTAGTG TTTCCCCAAA GAATGCCCAT CTGACTCAGA ATGGCACAGG CAGCCTCTCC 
AGGGGATTGG CAGTAACCAT GCAAGACATT CAAAGCAAGA GACAGCTGGC ACTGCAACAA 
AAAGACAGTC TGAGCACAGA AGATTCTCTT CCATCTGAAG TTGAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821621.1
CDS	63..4619
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966167.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966167.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA 
GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG 
AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA 
CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA 
ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA 
GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT 
CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG 
TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG 
GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA 
CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG 
GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT 
CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT 
CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT 
GCTCTCCGAG AGGATGCTCT TCAAATCTAT GAGACTAGAG ACTGCTTACT CGCTCAAGCT 
GATGTTTCTT GCACACCTGC TATCCCTTCT GCCTGCTCTC ACTCCCCTTC CCGGCAGCAA 
GAGGAATACC TGTGCCTCTC TGATGTTCTC CGCTTGTACA GCAGCTCGCC TCTTGTTCCT 
GCTTTTCCCC TTCTCTCTGC TGCAGAGCCC ACCGCCGCCC TTGAGTACAT CACTTTAGAT 
CAGCTGCAGG ATATTCTGGA AAGCATGCCC ACAGGTTCTG CTCCAACTGC TGTGGCAATG 
GCTGGCACTG ATATCAACTC TGCCTCTTGT GCTCCTTCAC CTGACGACCC TCCTCCTGTA 
CATCTCTCGT CTTCATCTTC TTCCTCTCCC TCCATTTCTC TGGAGGTGAA TTCCCCAACA 
ACAGAAGGTC ACTCTGCCCC CCTAGCACCT GATTTGGATG CCTATTACCA AGATTTAGTA 
ACTGCACCTT CTGCCTCTTC TTCTCCATCT TCTTCCCCTC CTCCACTCCC TGCTAAATCC 
CACTCATCAC GTAAGGCTTT GCAGATTTCT CGCTCTCGGA CAGAAATTGA GGGTGGAGGC 
ACTCTTCCAC GGATGAAAAC AGTCTCCCCC GCTTCTTCTC ACTTTTCAGT GGCCACTCTG 
GGCCGTAGTG TTTCCCCAAA GAATGCCCAT CTGACTCAGA ATGGCACAGG CAGCCTCTCC 
AGGGGATTGG CAGTAACCAT GCAAGACATT CAAAGCAAGA GACAGCTGGC ACTGCAACAA 
AAAGACAGTC TGAGCACAGA AGATTCTCTT CCATCTGAAG TTGAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966168.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821622.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X3, mRNA.

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CloneID	OXa32406
Clone ID Related Accession (Same CDS sequence)	XM_012966168.2 , XM_012966168.3
Accession Version	XM_012966168.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGAG 
TCTGAACTCC TGGAGACGAA GATGAAGCTG TTTGAGGATC TGGAGTTCCA GCAGCTAGAA 
AAGGAAAGCC GCCTGGAAGA AGAAAGAGAG ACCACAAGTC AACAACTTTT ACAGAGCAAG 
GGGGAATGCC AGCGAAGCAT TGCACGCAGG AAGGAACGCC TGGCTGCTTT GGAGATGCAA 
GCCAATCAGA TTCGCCTCCA GGCAGCACAG GATGCAGACC ATCTTGGCCA GGAAAGAAGC 
GCCATACTTT TACAGCTACA CAAGGAGAAA GAGAAGCTGG TTCAGTTGGA GAGAAGATAC 
CAGGCACTAA CAGGACGACG GGGATTCTCA ACTGGATCAG CTGCTCTCCG AGAGGATGCT 
CTTCAAATCT ATGAGACTAG AGACTGCTTA CTCGCTCAAG CTGATGTTTC TTGCACACCT 
GCTATCCCTT CTGCCTGCTC TCACTCCCCT TCCCGGCAGC AAGAGGAATA CCTGTGCCTC 
TCTGATGTTC TCCGCTTGTA CAGCAGCTCG CCTCTTGTTC CTGCTTTTCC CCTTCTCTCT 
GCTGCAGAGC CCACCGCCGC CCTTGAGTAC ATCACTTTAG ATCAGCTGCA GGATATTCTG 
GAAAGCATGC CCACAGGTTC TGCTCCAACT GCTGTGGCAA TGGCTGGCAC TGATATCAAC 
TCTGCCTCTT GTGCTCCTTC ACCTGACGAC CCTCCTCCTG TACATCTCTC GTCTTCATCT 
TCTTCCTCTC CCTCCATTTC TCTGGAGGTG AATTCCCCAA CAACAGAAGG TCACTCTGCC 
CCCCTAGCAC CTGATTTGGA TGCCTATTAC CAAGATTTAG TAACTGCACC TTCTGCCTCT 
TCTTCTCCAT CTTCTTCCCC TCCTCCACTC CCTGCTAAAT CCCACTCATC ACGTAAGGCT 
TTGCAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA 
GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT 
CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT 
GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG 
CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT 
TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG 
TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA 
GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA 
AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG 
ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA 
CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC 
CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG 
ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG 
TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG 
CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT 
GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT 
GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT 
CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT 
GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821622.1
CDS	64..4602
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966168.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966168.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGAG 
TCTGAACTCC TGGAGACGAA GATGAAGCTG TTTGAGGATC TGGAGTTCCA GCAGCTAGAA 
AAGGAAAGCC GCCTGGAAGA AGAAAGAGAG ACCACAAGTC AACAACTTTT ACAGAGCAAG 
GGGGAATGCC AGCGAAGCAT TGCACGCAGG AAGGAACGCC TGGCTGCTTT GGAGATGCAA 
GCCAATCAGA TTCGCCTCCA GGCAGCACAG GATGCAGACC ATCTTGGCCA GGAAAGAAGC 
GCCATACTTT TACAGCTACA CAAGGAGAAA GAGAAGCTGG TTCAGTTGGA GAGAAGATAC 
CAGGCACTAA CAGGACGACG GGGATTCTCA ACTGGATCAG CTGCTCTCCG AGAGGATGCT 
CTTCAAATCT ATGAGACTAG AGACTGCTTA CTCGCTCAAG CTGATGTTTC TTGCACACCT 
GCTATCCCTT CTGCCTGCTC TCACTCCCCT TCCCGGCAGC AAGAGGAATA CCTGTGCCTC 
TCTGATGTTC TCCGCTTGTA CAGCAGCTCG CCTCTTGTTC CTGCTTTTCC CCTTCTCTCT 
GCTGCAGAGC CCACCGCCGC CCTTGAGTAC ATCACTTTAG ATCAGCTGCA GGATATTCTG 
GAAAGCATGC CCACAGGTTC TGCTCCAACT GCTGTGGCAA TGGCTGGCAC TGATATCAAC 
TCTGCCTCTT GTGCTCCTTC ACCTGACGAC CCTCCTCCTG TACATCTCTC GTCTTCATCT 
TCTTCCTCTC CCTCCATTTC TCTGGAGGTG AATTCCCCAA CAACAGAAGG TCACTCTGCC 
CCCCTAGCAC CTGATTTGGA TGCCTATTAC CAAGATTTAG TAACTGCACC TTCTGCCTCT 
TCTTCTCCAT CTTCTTCCCC TCCTCCACTC CCTGCTAAAT CCCACTCATC ACGTAAGGCT 
TTGCAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA 
GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT 
CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT 
GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG 
CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT 
TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG 
TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA 
GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA 
AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG 
ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA 
CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC 
CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG 
ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG 
TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG 
CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT 
GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT 
GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT 
CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT 
GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966169.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821623.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa32407
Clone ID Related Accession (Same CDS sequence)	XM_012966169.3 , XM_012966169.2
Accession Version	XM_012966169.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821623.1
CDS	63..4583
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966169.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966169.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966170.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821624.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa32408
Clone ID Related Accession (Same CDS sequence)	XM_012966170.3 , XM_012966170.2
Accession Version	XM_012966170.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT 
AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821624.1
CDS	63..4580
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966170.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966170.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT 
AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966171.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821625.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X6, mRNA.

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CloneID	OXa32409
Clone ID Related Accession (Same CDS sequence)	XM_012966171.2 , XM_012966171.3
Accession Version	XM_012966171.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821625.1
CDS	62..4552
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966171.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966171.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG 
ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA 
TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC 
TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT 
GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT 
GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA 
ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG 
CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG 
GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG 
ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG 
GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT 
CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA 
AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT 
GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT 
TATTATGACC ACCTCAGGAG TGCAGCCAAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966172.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821626.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X7, mRNA.

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CloneID	OXa32410
Clone ID Related Accession (Same CDS sequence)	XM_012966172.3 , XM_012966172.2
Accession Version	XM_012966172.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGTACA TCACTTTAGA TCAGCTGCAG GATATTCTGG AAAGCATGCC CACAGGTTCT 
GCTCCAACTG CTGTGGCAAT GGCTGGCACT GATATCAACT CTGCCTCTTG TGCTCCTTCA 
CCTGACGACC CTCCTCCTGT ACATCTCTCG TCTTCATCTT CTTCCTCTCC CTCCATTTCT 
CTGGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT 
GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT 
CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG 
ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT 
CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG 
AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG 
AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA 
GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT 
AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821626.1
CDS	63..4520
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966172.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966172.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGTACA TCACTTTAGA TCAGCTGCAG GATATTCTGG AAAGCATGCC CACAGGTTCT 
GCTCCAACTG CTGTGGCAAT GGCTGGCACT GATATCAACT CTGCCTCTTG TGCTCCTTCA 
CCTGACGACC CTCCTCCTGT ACATCTCTCG TCTTCATCTT CTTCCTCTCC CTCCATTTCT 
CTGGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT 
GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT 
CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG 
ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT 
CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG 
AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG 
AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA 
GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT 
AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT 
CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT 
CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC 
ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966173.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821627.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa32411
Clone ID Related Accession (Same CDS sequence)	XM_012966173.3 , XM_012966173.2
Accession Version	XM_012966173.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAG TCCTAACCCA 
GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA 
GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC 
ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821627.1
CDS	61..4509
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966173.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966173.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA 
ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA 
GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA 
TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA 
GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT 
CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC 
TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA 
CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA 
GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC 
ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT 
GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT 
GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC 
ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG 
CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG 
CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA 
GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC 
TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT 
GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA 
AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA 
CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC 
CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAG TCCTAACCCA 
GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA 
GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC 
ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966174.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821628.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa32412
Clone ID Related Accession (Same CDS sequence)	XM_012966174.2 , XM_012966174.1
Accession Version	XM_012966174.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC TGAGTCATGA TGTGAGCAGC CCCTGCAGAA AAACACACAC AGTTATTCAG 
AGCACACCAT TGGATCTCAT AGATACAGGA AAAAGTCTTA GGGTGCAGAC AGAAAAACCT 
CATCTTGTCA GCCTGGGAAG TGGACGACTC AGCACTGCAA TCACCCTACT ACCACTGGAA 
GAAGGAAGGA CAACCCTGGG TCATGCATCT AAGGACATTC CCCTGCAGGG ACCTGGCATT 
GCTGCAGAAC ATTGCTACAT TGAAAACATC AGAGGAAACC TCACCCTATA CCCATGTGGA 
AATCCTTGTG CCATCGATGG ACTGCTCATC ACCCAACCGA CACGTCTCAC TCAAGGGTGT 
ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT 
TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC 
CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT 
TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC 
CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC 
TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT 
AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA 
ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT 
GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA 
ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT 
CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG 
AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG 
CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA 
GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT 
GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA 
TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG 
CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC 
AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG 
CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA 
ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC 
CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC 
TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT 
AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT 
ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA 
CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA 
GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC 
CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC 
CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG 
GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC 
GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG 
CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821628.1
CDS	168..4598
Translation	MKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966174.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966174.1

ORF Insert Sequence:

ATGAAGGCAC TGAGTCATGA TGTGAGCAGC CCCTGCAGAA AAACACACAC AGTTATTCAG 
AGCACACCAT TGGATCTCAT AGATACAGGA AAAAGTCTTA GGGTGCAGAC AGAAAAACCT 
CATCTTGTCA GCCTGGGAAG TGGACGACTC AGCACTGCAA TCACCCTACT ACCACTGGAA 
GAAGGAAGGA CAACCCTGGG TCATGCATCT AAGGACATTC CCCTGCAGGG ACCTGGCATT 
GCTGCAGAAC ATTGCTACAT TGAAAACATC AGAGGAAACC TCACCCTATA CCCATGTGGA 
AATCCTTGTG CCATCGATGG ACTGCTCATC ACCCAACCGA CACGTCTCAC TCAAGGGTGT 
ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT 
TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC 
CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT 
TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC 
CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC 
TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT 
AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA 
ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT 
GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA 
ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT 
CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG 
AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG 
CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA 
GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT 
GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA 
TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG 
CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC 
AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG 
CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA 
ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC 
CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC 
TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT 
AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT 
ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA 
CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA 
GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC 
CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC 
CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG 
GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC 
GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG 
CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966175.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821629.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X10, mRNA.

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CloneID	OXa32413
Clone ID Related Accession (Same CDS sequence)	XM_012966175.2 , XM_012966175.3
Accession Version	XM_012966175.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821629.1
CDS	64..4494
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966175.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966175.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966176.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821630.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X11, mRNA.

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CloneID	OXa32414
Clone ID Related Accession (Same CDS sequence)	XM_012966176.2 , XM_012966176.3
Accession Version	XM_012966176.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGAT TTCTCGCTCT 
CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT 
TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT 
CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC 
AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT 
GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG 
AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG 
TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA 
GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT 
AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA 
GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC 
AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG 
AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA 
CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA 
CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC 
CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG 
ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG 
AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT 
TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT 
TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821630.1
CDS	62..4462
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966176.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966176.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA 
GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA 
ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT 
GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGAT TTCTCGCTCT 
CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT 
TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT 
CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC 
AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT 
GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG 
AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG 
TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA 
GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT 
AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA 
GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC 
AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG 
AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA 
CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA 
CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC 
CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG 
ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG 
AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT 
TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT 
TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966177.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821631.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OXa32415
Clone ID Related Accession (Same CDS sequence)	XM_012966177.2 , XM_012966177.3
Accession Version	XM_012966177.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGAT TTCTCGCTCT 
CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT 
TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT 
CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC 
AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT 
GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG 
AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG 
TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA 
GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT 
AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA 
GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC 
AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG 
AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA 
CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA 
CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC 
CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG 
ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG 
AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT 
TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT 
TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821631.1
CDS	61..4281
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966177.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966177.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT 
CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGAT TTCTCGCTCT 
CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT 
TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT 
CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC 
AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT 
GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG 
AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG 
TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA 
GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT 
AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA 
GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC 
AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG 
AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA 
CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA 
CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC 
CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG 
ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG 
AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT 
TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT 
TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG 
AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA 
GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT 
TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966178.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821632.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OXa32416
Clone ID Related Accession (Same CDS sequence)	XM_012966178.2 , XM_012966178.3
Accession Version	XM_012966178.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA 
GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT 
CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT 
GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG 
CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT 
TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG 
TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA 
GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA 
AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG 
ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA 
CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC 
CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG 
ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG 
TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG 
CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT 
GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT 
GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT 
CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT 
GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821632.1
CDS	59..4177
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966178.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966178.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA 
GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT 
CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT 
GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG 
CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT 
TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG 
TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA 
GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA 
AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG 
ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA 
CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC 
CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG 
ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG 
TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG 
CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT 
GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT 
GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT 
CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT 
GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966179.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821633.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X15, mRNA.

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CloneID	OXa32417
Clone ID Related Accession (Same CDS sequence)	XM_012966179.2 , XM_012966179.3
Accession Version	XM_012966179.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821633.1
CDS	58..4134
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966179.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966179.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA 
GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG 
CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA 
ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA 
AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC 
ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA 
GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT 
CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC 
ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC 
ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA 
TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG 
GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT 
GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA 
AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG 
AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA 
GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC 
ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA 
TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA 
GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT 
TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG 
ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC 
TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG 
ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966180.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821634.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X16, mRNA.

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CloneID	OXa32418
Clone ID Related Accession (Same CDS sequence)	XM_012966180.2 , XM_012966180.3
Accession Version	XM_012966180.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT 
TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC 
CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT 
TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC 
CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC 
TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT 
AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA 
ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT 
GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA 
ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT 
CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG 
AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG 
CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA 
GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT 
GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA 
TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG 
CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC 
AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG 
CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA 
ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC 
CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC 
TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT 
AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT 
ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA 
CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA 
GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC 
CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC 
CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG 
GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC 
GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG 
CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821634.1
CDS	645..4715
Translation	MVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966180.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966180.2

ORF Insert Sequence:

ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT 
TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC 
CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT 
TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC 
CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC 
TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT 
AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA 
ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT 
GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA 
ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT 
CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG 
AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG 
CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA 
GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT 
GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA 
TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG 
CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC 
AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG 
CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA 
ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC 
CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC 
TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT 
AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT 
ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA 
CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA 
GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC 
CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC 
CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG 
GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC 
GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG 
CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC 
AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG 
CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG 
GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA 
CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC 
AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC 
CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA 
ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG 
GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT 
CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG 
AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT 
GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT 
TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG 
AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA 
CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC 
CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC 
GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC 
AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG 
ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966182.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821636.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OXa32419
Clone ID Related Accession (Same CDS sequence)	XM_012966182.3 , XM_012966182.2
Accession Version	XM_012966182.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA 
CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT 
GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG 
GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGACAGT 
CTGAGCACAG AAGATTCTCT TCCATCTGAA GTTGAAGGTC AACAAGTGAT AGAGGAGCAG 
CGCAGACGTC TTGCAGAGTT GAAACAGAGA GCTGCAGTTG AGGCACAATC TCAGTGGGAA 
TCTCTCCATG GAGCCCCTAA TCAAGGGTAC ACGCCTATAA TTCACCACTC CATTCTACAC 
CATCAGCGGC CTCTCCATTC TCGTGAAGAT GACCCTACCC ATGGATTTGA CACTCTCAGT 
CTGGAGAGTT CTGATAGTTT GGACACTAGT GTTTCTACAG GAAACTCTGC ATGTTCCCCT 
GATAACATGT CAAGTGCTAG TGGGTTAGAC ATGCTAAAGA TAGAGGAAAT GGAAAGAATG 
CTTCTAGAAG CTCATGCAGA GCGTGCCAGG CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG 
GCACGCAGAA AAGCACTAGA AGATGAGAGG CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT 
CAAGATGAGA CAGCTCACAG GCAAAGACTA GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG 
CATTTTTCAC AGGCTCGTCC TCTTACACGC TATCTGCCAA TCCGGAAGGA AGACTTTGAT 
CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT GCAGTAGATG CCTGCCCTCA CATCATTTTA 
AGTGAGAAGA TGTGTCGTGG CTTCCTGACA AAAATGGGTG GAAAGATAAA ATCATGGAAG 
AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA CGGACCCTGT CCTATTATGT AGATAAACAT 
GAGGCAAAGC TGAAAGGAGT TATATATTTC CAGGCCATAG AAGAGGTTTA TTATGACCAC 
CTCAGGAGTG CAGCCAAGAA AGGACTTTTT AACCTCAGCC TGGCCACTCA GATATCCGAT 
TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG 
ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG 
GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821636.1
CDS	59..4066
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKDSLSTEDSLPSEVEGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966182.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966182.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT 
TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG 
CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT 
CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT 
CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC 
CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC 
CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG 
GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA 
GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA 
CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT 
GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG 
GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGACAGT 
CTGAGCACAG AAGATTCTCT TCCATCTGAA GTTGAAGGTC AACAAGTGAT AGAGGAGCAG 
CGCAGACGTC TTGCAGAGTT GAAACAGAGA GCTGCAGTTG AGGCACAATC TCAGTGGGAA 
TCTCTCCATG GAGCCCCTAA TCAAGGGTAC ACGCCTATAA TTCACCACTC CATTCTACAC 
CATCAGCGGC CTCTCCATTC TCGTGAAGAT GACCCTACCC ATGGATTTGA CACTCTCAGT 
CTGGAGAGTT CTGATAGTTT GGACACTAGT GTTTCTACAG GAAACTCTGC ATGTTCCCCT 
GATAACATGT CAAGTGCTAG TGGGTTAGAC ATGCTAAAGA TAGAGGAAAT GGAAAGAATG 
CTTCTAGAAG CTCATGCAGA GCGTGCCAGG CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG 
GCACGCAGAA AAGCACTAGA AGATGAGAGG CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT 
CAAGATGAGA CAGCTCACAG GCAAAGACTA GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG 
CATTTTTCAC AGGCTCGTCC TCTTACACGC TATCTGCCAA TCCGGAAGGA AGACTTTGAT 
CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT GCAGTAGATG CCTGCCCTCA CATCATTTTA 
AGTGAGAAGA TGTGTCGTGG CTTCCTGACA AAAATGGGTG GAAAGATAAA ATCATGGAAG 
AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA CGGACCCTGT CCTATTATGT AGATAAACAT 
GAGGCAAAGC TGAAAGGAGT TATATATTTC CAGGCCATAG AAGAGGTTTA TTATGACCAC 
CTCAGGAGTG CAGCCAAGAA AGGACTTTTT AACCTCAGCC TGGCCACTCA GATATCCGAT 
TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG 
ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG 
GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966186.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821640.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OXa32423
Clone ID Related Accession (Same CDS sequence)	XM_012966186.2
Accession Version	XM_012966186.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966186.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA 
GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG 
AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA 
CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA 
ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA 
GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT 
CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG 
TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG 
GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA 
CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG 
GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT 
CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT 
CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT 
GCTCTCCGAG AGGATGCTCT TCAAATCTAT GAGACTAGAG ACTGCTTACT CGCTCAAGCT 
GATGTTTCTT GCACACCTGC TATCCCTTCT GCCTGCTCTC ACTCCCCTTC CCGGCAGCAA 
GAGGAATACC TGTGCCTCTC TGATGTTCTC CGCTTGTACA GCAGCTCGCC TCTTGTTCCT 
GCTTTTCCCC TTCTCTCTGC TGCAGAGCCC ACCGCCGCCC TTGAGTACAT CACTTTAGAT 
CAGCTGCAGG ATATTCTGGA AAGCATGCCC ACAGGTTCTG CTCCAACTGC TGTGGCAATG 
GCTGGCACTG ATATCAACTC TGCCTCTTGT GCTCCTTCAC CTGACGACCC TCCTCCTGTA 
CATCTCTCGT CTTCATCTTC TTCCTCTCCC TCCATTTCTC TGGAGGTGAA TTCCCCAACA 
ACAGAAGGTC ACTCTGCCCC CCTAGCACCT GATTTGGATG CCTATTACCA AGATTTAGTA 
ACTGCACCTT CTGCCTCTTC TTCTCCATCT TCTTCCCCTC CTCCACTCCC TGCTAAATCC 
CACTCATCAC GTAAGGCTTT GCAGATTTCT CGCTCTCGGA CAGAAATTGA GGGTGGAGGC 
ACTCTTCCAC GGATGAAAAC AGTCTCCCCC GCTTCTTCTC ACTTTTCAGT GGCCACTCTG 
GGCCGTAGTG TTTCCCCAAA GAATGCCCAT CTGACTCAGA ATGGCACAGG CAGCCTCTCC 
AGGGGATTGG CAGTAACCAT GCAAGACATT CAAAGCAAGA GACAGCTGGC ACTGCAACAA 
AAAGGTCAAC AAGTGATAGA GGAGCAGCGC AGACGTCTTG CAGAGTTGAA ACAGAGAGCT 
GCAGTTGAGG CACAATCTCA GTGGGAATCT CTCCATGGAG CCCCTAATCA AGGGTACACG 
CCTATAATTC ACCACTCCAT TCTACACCAT CAGCGGCCTC TCCATTCTCG TGAAGATGAC 
CCTACCCATG GATTTGACAC TCTCAGTCTG GAGAGTTCTG ATAGTTTGGA CACTAGTGTT 
TCTACAGGAA ACTCTGCATG TTCCCCTGAT AACATGTCAA GTGCTAGTGG GTTAGACATG 
CTAAAGATAG AGGAAATGGA AAGAATGCTT CTAGAAGCTC ATGCAGAGCG TGCCAGGCTT 
GTAGAATCCA GGGAGCGTGA GGTGGAGGCA CGCAGAAAAG CACTAGAAGA TGAGAGGCGA 
AGGAGAGAGG AGCTGGAAAG GCGTCTTCAA GATGAGACAG CTCACAGGCA AAGACTAGTA 
GAGAAGGAGG TCAAGATGAG GGAGAAGCAT TTTTCACAGG CTCGTCCTCT TACACGCTAT 
CTGCCAATCC GGAAGGAAGA CTTTGATCTC CGAGCCCATG TTGAGTCCTC AGGCCATGCA 
GTAGATGCCT GCCCTCACAT CATTTTAAGT GAGAAGATGT GTCGTGGCTT CCTGACAAAA 
ATGGGTGGAA AGATAAAATC ATGGAAGAAG CGCTGGTTTG TTTTTGATCG CCTGAAACGG 
ACCCTGTCCT ATTATGTAGA TAAACATGAG GCAAAGCTGA AAGGAGTTAT ATATTTCCAG 
GCCATAGAAG AGGTTTATTA TGACCACCTC AGGAGTGCAG CCAAGAGTCC TAACCCAGCT 
CTAACATTCT GTGTCAAGAC TCACGATCGC CTGTATTACA TGGTAGCTCC TTCCCCAGAG 
GCTATGCGGA TATGGATGGA TGTCATTGTA ACTGGGGCAG AGGGTTACAC TCAGTTCATG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821640.1
CDS	60..4505
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKAREKLEQERMELQSIQLSHHDLQRQLHNCPEALREQVQCQLTRESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966186.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966186.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA 
GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG 
AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA 
CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA 
ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA 
GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT 
CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG 
TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG 
GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA 
CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG 
GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT 
CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT 
CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT 
GCTCTCCGAG AGGATGCTCT TCAAATCTAT GAGACTAGAG ACTGCTTACT CGCTCAAGCT 
GATGTTTCTT GCACACCTGC TATCCCTTCT GCCTGCTCTC ACTCCCCTTC CCGGCAGCAA 
GAGGAATACC TGTGCCTCTC TGATGTTCTC CGCTTGTACA GCAGCTCGCC TCTTGTTCCT 
GCTTTTCCCC TTCTCTCTGC TGCAGAGCCC ACCGCCGCCC TTGAGTACAT CACTTTAGAT 
CAGCTGCAGG ATATTCTGGA AAGCATGCCC ACAGGTTCTG CTCCAACTGC TGTGGCAATG 
GCTGGCACTG ATATCAACTC TGCCTCTTGT GCTCCTTCAC CTGACGACCC TCCTCCTGTA 
CATCTCTCGT CTTCATCTTC TTCCTCTCCC TCCATTTCTC TGGAGGTGAA TTCCCCAACA 
ACAGAAGGTC ACTCTGCCCC CCTAGCACCT GATTTGGATG CCTATTACCA AGATTTAGTA 
ACTGCACCTT CTGCCTCTTC TTCTCCATCT TCTTCCCCTC CTCCACTCCC TGCTAAATCC 
CACTCATCAC GTAAGGCTTT GCAGATTTCT CGCTCTCGGA CAGAAATTGA GGGTGGAGGC 
ACTCTTCCAC GGATGAAAAC AGTCTCCCCC GCTTCTTCTC ACTTTTCAGT GGCCACTCTG 
GGCCGTAGTG TTTCCCCAAA GAATGCCCAT CTGACTCAGA ATGGCACAGG CAGCCTCTCC 
AGGGGATTGG CAGTAACCAT GCAAGACATT CAAAGCAAGA GACAGCTGGC ACTGCAACAA 
AAAGGTCAAC AAGTGATAGA GGAGCAGCGC AGACGTCTTG CAGAGTTGAA ACAGAGAGCT 
GCAGTTGAGG CACAATCTCA GTGGGAATCT CTCCATGGAG CCCCTAATCA AGGGTACACG 
CCTATAATTC ACCACTCCAT TCTACACCAT CAGCGGCCTC TCCATTCTCG TGAAGATGAC 
CCTACCCATG GATTTGACAC TCTCAGTCTG GAGAGTTCTG ATAGTTTGGA CACTAGTGTT 
TCTACAGGAA ACTCTGCATG TTCCCCTGAT AACATGTCAA GTGCTAGTGG GTTAGACATG 
CTAAAGATAG AGGAAATGGA AAGAATGCTT CTAGAAGCTC ATGCAGAGCG TGCCAGGCTT 
GTAGAATCCA GGGAGCGTGA GGTGGAGGCA CGCAGAAAAG CACTAGAAGA TGAGAGGCGA 
AGGAGAGAGG AGCTGGAAAG GCGTCTTCAA GATGAGACAG CTCACAGGCA AAGACTAGTA 
GAGAAGGAGG TCAAGATGAG GGAGAAGCAT TTTTCACAGG CTCGTCCTCT TACACGCTAT 
CTGCCAATCC GGAAGGAAGA CTTTGATCTC CGAGCCCATG TTGAGTCCTC AGGCCATGCA 
GTAGATGCCT GCCCTCACAT CATTTTAAGT GAGAAGATGT GTCGTGGCTT CCTGACAAAA 
ATGGGTGGAA AGATAAAATC ATGGAAGAAG CGCTGGTTTG TTTTTGATCG CCTGAAACGG 
ACCCTGTCCT ATTATGTAGA TAAACATGAG GCAAAGCTGA AAGGAGTTAT ATATTTCCAG 
GCCATAGAAG AGGTTTATTA TGACCACCTC AGGAGTGCAG CCAAGAGTCC TAACCCAGCT 
CTAACATTCT GTGTCAAGAC TCACGATCGC CTGTATTACA TGGTAGCTCC TTCCCCAGAG 
GCTATGCGGA TATGGATGGA TGTCATTGTA ACTGGGGCAG AGGGTTACAC TCAGTTCATG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phldb1 ( XM_012966188.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821642.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X22, mRNA.

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CloneID	OXa32424
Clone ID Related Accession (Same CDS sequence)	XM_012966188.3 , XM_012966188.2
Accession Version	XM_012966188.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology-like domain family B member 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGGT 
CAACAAGTGA TAGAGGAGCA GCGCAGACGT CTTGCAGAGT TGAAACAGAG AGCTGCAGTT 
GAGGCACAAT CTCAGTGGGA ATCTCTCCAT GGAGCCCCTA ATCAAGGGTA CACGCCTATA 
ATTCACCACT CCATTCTACA CCATCAGCGG CCTCTCCATT CTCGTGAAGA TGACCCTACC 
CATGGATTTG ACACTCTCAG TCTGGAGAGT TCTGATAGTT TGGACACTAG TGTTTCTACA 
GGAAACTCTG CATGTTCCCC TGATAACATG TCAAGTGCTA GTGGGTTAGA CATGCTAAAG 
ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA GCTCATGCAG AGCGTGCCAG GCTTGTAGAA 
TCCAGGGAGC GTGAGGTGGA GGCACGCAGA AAAGCACTAG AAGATGAGAG GCGAAGGAGA 
GAGGAGCTGG AAAGGCGTCT TCAAGATGAG ACAGCTCACA GGCAAAGACT AGTAGAGAAG 
GAGGTCAAGA TGAGGGAGAA GCATTTTTCA CAGGCTCGTC CTCTTACACG CTATCTGCCA 
ATCCGGAAGG AAGACTTTGA TCTCCGAGCC CATGTTGAGT CCTCAGGCCA TGCAGTAGAT 
GCCTGCCCTC ACATCATTTT AAGTGAGAAG ATGTGTCGTG GCTTCCTGAC AAAAATGGGT 
GGAAAGATAA AATCATGGAA GAAGCGCTGG TTTGTTTTTG ATCGCCTGAA ACGGACCCTG 
TCCTATTATG TAGATAAACA TGAGGCAAAG CTGAAAGGAG TTATATATTT CCAGGCCATA 
GAAGAGGTTT ATTATGACCA CCTCAGGAGT GCAGCCAAGA AAGGACTTTT TAACCTCAGC 
CTGGCCACTC AGATATCCGA TTTTCTTTCT GTGCCCCTGT GGAAGGAGAG TCCTAACCCA 
GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA 
GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC 
ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821642.1
CDS	63..4451
Translation	MERCWGPLTEFYHRIACTCWHQGRGGQDYLETEFPAQTEIEDTMKALSHDVSSPCRKTHTVIQSTPLDLIDTGKSLRVQTEKPHLVSLGSGRLSTAITLLPLEEGRTTLGHASKDIPLQGPGIAAEHCYIENIRGNLTLYPCGNPCAIDGLLITQPTRLTQGCMVCLGQSTFLRFNHPAEAHWMKTMIPSTGRSPAPGLYPSDSSSSLLNGSRDAAYPRTVPSHTSLVSSIEKNLQDIMDSLVLEGPAPLSRKLSQTSLNHITPMTNGENRRYLLSSPTSPPYSNSGYDNTSPLSSPGSGSLTSPSPGQDAGTPTAPPVVPLRTSSFSHSLPRPVPTYGGSNLDLRSNRNTGGGSLLSPSSPATGSRMGGLSVPSSPLLGSKFHHQITDRPTSPFRQVVGPTRSLPPESPRLGRRNFESMRDLPPLSPSLPRRSSSSSIKPAPDSPSRVPDSPSTLGRRRVGSASSPGTENNASTRQIRERSPSPSSLQETSRRLTPAYSLGSLGTPSQSPRAQRKGPVLRERKNSISEISGNQEELMDYHRRQREERLREQEMERLERQRLETILNLCAEYSKTDDTAPPARLDEIRSFPTLGGTEETTASLFTRRPSWESTRSTNLEGTGNRVRAQGEHNEEETQREENSSTESTQHEQEDAVSPTGAAELLALEEQRRHTLGHVDQLKNRVTELEGQLQESAREAEMERALLQGEREAELSQLQHEQRMVQQLQDSLTSLENNLQKEKDKESELLETKMKLFEDLEFQQLEKESRLEEERETTSQQLLQSKGECQRSIARRKERLAALEMQANQIRLQAAQDADHLGQERSAILLQLHKEKEKLVQLERRYQALTGRRGFSTGSAALREDALQIYETRDCLLAQADVSCTPAIPSACSHSPSRQQEEYLCLSDVLRLYSSSPLVPAFPLLSAAEPTAALEYITLDQLQDILESMPTGSAPTAVAMAGTDINSASCAPSPDDPPPVHLSSSSSSSPSISLEVNSPTTEGHSAPLAPDLDAYYQDLVTAPSASSSPSSSPPPLPAKSHSSRKALQISRSRTEIEGGGTLPRMKTVSPASSHFSVATLGRSVSPKNAHLTQNGTGSLSRGLAVTMQDIQSKRQLALQQKGQQVIEEQRRRLAELKQRAAVEAQSQWESLHGAPNQGYTPIIHHSILHHQRPLHSREDDPTHGFDTLSLESSDSLDTSVSTGNSACSPDNMSSASGLDMLKIEEMERMLLEAHAERARLVESREREVEARRKALEDERRRREELERRLQDETAHRQRLVEKEVKMREKHFSQARPLTRYLPIRKEDFDLRAHVESSGHAVDACPHIILSEKMCRGFLTKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHEAKLKGVIYFQAIEEVYYDHLRSAAKKGLFNLSLATQISDFLSVPLWKESPNPALTFCVKTHDRLYYMVAPSPEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_012966188.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966188.2

ORF Insert Sequence:

ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA 
GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA 
GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA 
GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA 
CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA 
CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC 
CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT 
CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA 
GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC 
CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT 
GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC 
TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC 
CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT 
CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA 
AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG 
CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA 
AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA 
AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT 
AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA 
CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG 
AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA 
CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG 
GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG 
CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC 
CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA 
CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC 
CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG 
AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC 
CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT 
GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA 
ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG 
AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT 
GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA 
AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG 
GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA 
AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG 
AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC 
CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT 
TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT 
TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC 
CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG 
GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC 
CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT 
TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA 
TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT 
CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG 
CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC 
ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC 
TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA 
GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA 
CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA 
TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT 
CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT 
AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA 
TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGGT 
CAACAAGTGA TAGAGGAGCA GCGCAGACGT CTTGCAGAGT TGAAACAGAG AGCTGCAGTT 
GAGGCACAAT CTCAGTGGGA ATCTCTCCAT GGAGCCCCTA ATCAAGGGTA CACGCCTATA 
ATTCACCACT CCATTCTACA CCATCAGCGG CCTCTCCATT CTCGTGAAGA TGACCCTACC 
CATGGATTTG ACACTCTCAG TCTGGAGAGT TCTGATAGTT TGGACACTAG TGTTTCTACA 
GGAAACTCTG CATGTTCCCC TGATAACATG TCAAGTGCTA GTGGGTTAGA CATGCTAAAG 
ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA GCTCATGCAG AGCGTGCCAG GCTTGTAGAA 
TCCAGGGAGC GTGAGGTGGA GGCACGCAGA AAAGCACTAG AAGATGAGAG GCGAAGGAGA 
GAGGAGCTGG AAAGGCGTCT TCAAGATGAG ACAGCTCACA GGCAAAGACT AGTAGAGAAG 
GAGGTCAAGA TGAGGGAGAA GCATTTTTCA CAGGCTCGTC CTCTTACACG CTATCTGCCA 
ATCCGGAAGG AAGACTTTGA TCTCCGAGCC CATGTTGAGT CCTCAGGCCA TGCAGTAGAT 
GCCTGCCCTC ACATCATTTT AAGTGAGAAG ATGTGTCGTG GCTTCCTGAC AAAAATGGGT 
GGAAAGATAA AATCATGGAA GAAGCGCTGG TTTGTTTTTG ATCGCCTGAA ACGGACCCTG 
TCCTATTATG TAGATAAACA TGAGGCAAAG CTGAAAGGAG TTATATATTT CCAGGCCATA 
GAAGAGGTTT ATTATGACCA CCTCAGGAGT GCAGCCAAGA AAGGACTTTT TAACCTCAGC 
CTGGCCACTC AGATATCCGA TTTTCTTTCT GTGCCCCTGT GGAAGGAGAG TCCTAACCCA 
GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA 
GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC 
ATGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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phldb1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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