spag17 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	spag17
Entrez Gene ID	100170502
Full Name	sperm associated antigen 17
General protein information	Preferred Names sperm associated antigen 17 Names sperm-associated antigen 17
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001130282.1	NP_001123754.1	sperm-associated antigen 17

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100170502	NP_001123754.1
Homo sapiens (human)	SPAG17	NP_996879.1
Pan troglodytes (chimpanzee)	SPAG17	XP_513687.2
	SPAG17	XP_002801770.1
Canis lupus familiaris (dog)	SPAG17	XP_849615.1
Mus musculus (house mouse)	Spag17	NP_083168.3
Gallus gallus (chicken)	SPAG17	XP_416538.4
Bos taurus (cattle)	SPAG17	NP_001193803.1
Rattus norvegicus (Norway rat)	Spag17	XP_006233092.1
Danio rerio (zebrafish)	si:dkey-208b23.5	XP_689610.5

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following spag17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spag17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa22256	NM_001130282.1 Latest version!	Xenopus tropicalis sperm associated antigen 17 (spag17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spag17 ( NM_001130282.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123754.1 Xenopus tropicalis sperm associated antigen 17 (spag17), mRNA.

Price & Availability↑

CloneID	OXa22256
Clone ID Related Accession (Same CDS sequence)	NM_001130282.1
Accession Version	NM_001130282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sperm-associated antigen 17
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167470.1. ##Evidence-Data-START## CDS exon combination :: BC167470.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00028291, SAMD00028292 [ECO:0000350] ##Evidence-Data-END##

ATGGGCCCGA AAAGAGCGAA GAGCACCGTC CCCCCGGCGG GTAGTGGCCC TGTGGGGGCC 
CCGGGTGTGG GCTCTCGGAG CTGGGAGTCC GGCCTTATCC TGGCGCAGCT GGAGCAGGAT 
GACTGGAGGG TCAGCATCGC CTTTGTCATT GAGAACCGCC CCGAGGATGA AGCCCACACG 
AAGGCGCTGT CCCAGGCTGT GTCCGTGCCG CTGCGCCAGC TCTTCAGTGT GGTGTCGTGG 
GCCGACATGA TCCGACAGGT CCAGGAGCTG GGAAATCCGA AGGTCAAGAA AACAAAGGAG 
TCGCCATTAT ATTGGGAGGT CACTGAGGTG GCCAAACTGC TGTTGGATCA CGGAGAAAAA 
CTGCCCCTGT CCCTGATTGC AAAACTGCTG AAATTCCAGT TCCTGAACAT CAAGCAGAAA 
GACCTGCAGA GGAGGGAGGC CGAGAAGAAG GCCCCAGAAG ACAAGTCGAA GAACAAACCC 
ACAAAGGCCA AATCACCCGG TGCCCGGTCT GCCGGCAAAT CAAAAGGAAA AAAAGGCCCA 
GAGGCTCCAC CCCCTGTCCA AAAAGTTACC TCCCTGAAGC GCCGGGGGGA AGAAGAAGAA 
ACCAACAGAT ATATAGATGA TGAGCCGGAC GATGGTGCCC AACATTACAT CATTGTCCTT 
GGCTTCTATC AGCCTCAGCT CGTGGCCCTG TTGGCCGAAC TAGGGATCTC CGTGTCCTGT 
ATTATAAGGA TATCGTCCCA GAACTACACG AGCCTTCCTG CCAATGAGCC GGATCCAACA 
GTGGCCCCTG AAGTTCTAGA GGCAGAACAG CTCAGGAAAG TGACTCTCAC AAAATCCTTG 
GAAACGTTCT GGAAGTACCT GGAGCCCATT CTGAACAGCG GCCCCCCGGG ATCCCGACTT 
CTGGAAGTGG CTCGGCTGCA CTATATGGTC AAGGAAAGGC ACTTTCCTCA GGACTGGGGT 
GACAGTGACC TCCTGTTGGC ATATGCCACA GAGCTGTATG AGAACGTTGC CTGCCTCATG 
TACGACTGTC TGGACTGGAG GCGGCAGCAC CGGCGCTACC TGCGTAACCT GCGGCTCATC 
CGCGTCCCCT CTGTGTCCGT AGGGAAGAGC CCAGACAGAC AGAACGTCCC GCCCGAGGCC 
CAGGAAGCTG ACCCCATCCC ATCATCCCCT ACAGGCAGGA GGAAAACTCA GACTGATGTG 
GCGCCTCCAG CTGCTTCTCT GCCACCGTCA TCTCCAATGC ACATGGAGGT CGAGGGCCCG 
AGTGGGGCGG CTGAGGTGGA TACGAGATTT TACACTGATC TCCTGAGCGA TCTCCCAGAG 
GAGCTTCAGT CGGTCCCAGT GATACTGCAC TGTGTGCTGG AACAGGTTGT GGCCACTACG 
GAGGATCTTG TGCCCCCCAG CCAAGCTGCA CCCAAGCCCC GCCCAGATGG GCTGGACCCC 
AATATTGCCA GTCACATGAT ATCTGTGTTG GATTCTCTCT CCCTGTCGGA CAAGGAAAAG 
AAGAACCTGT ACAACGCGTT CCGTGCCCAA GAGGCAGAGG GTGAGCGGGC AGAGGAGAGA 
CGGCCGGAGC TGCTGAACCA CCATGATAAG ATTGGGCACA GGGCCGCCCA GGCACAGCCC 
CCCAGCGCAT TGGATCCAGT CAGAATAGAG CAGGAAATGA TTGGAAAACT TCCCCTTGCG 
CAACTTCTGC GATTTCCCCA ACCTGCAGCG GAGAGGAACT CACGGAGACT CGCGCAGATA 
CATGAACTCA TGCACTACTG CACGTCTGAC CTGACACCCT GGGAAGGAGT CTCGTGGGCA 
TTCAAGCTGT TCACGTTGGA GAGCCTCCCG CTGACTGGAT TGGATGACCT GGGGGAAGTG 
GAGGGCAGTG GGGTGGAGGT GCAGCAAGAT GGCGACATTC CGTGGGACGA TCCCGCCAGA 
TTCGCTCGCA CACTGGGCAG GATGGAAAGT GTGAGGCGGC TGCACGGGCC CAAAGCGCCA 
GAACCTGAAG GGAATTCTTC CGGGCAAAGT CAAGATCAGG AGGAAACGGC AGGAGCAGAA 
AAGGAGATTT CCCCAGAGGA GGAGGCCGCA CAGACACAAC TGGGAGACAT TAGGAGGACA 
CAGAGAAGAT CTCTGAGTGA CTGGTGCTAC TGGGAGCATT TTAATTCAGA ACTACTCAAG 
CAGGTGGTGT GTGACGCCGC TCAGGCCTAC AGGTGTGTGG ATTACTATTA TCACTCTCAG 
GATAACTCTC TGCTCCTCGT TATGCACAAT CCCATGAACC CCTACCATCA GAGCGAGGAA 
TCCTGGGACA TGGCTCTCCA TTCCAACGTC AGTTTCAGAA ACTACCTGGA GTTGGTAGCA 
GAGTCCATTT CCAACTGGGT GAGGGAGGAG GAGGAGAAGT ATCAGGAGGA GATGGCAGTC 
AGAGAGTTGG AAGCTTTGAA ACAAATTCAA TCTAGGCAGG ATGGTGAGGC CCTACCAAAA 
GCCAACACCA AGAAATCCAA GAGATCCCCC TCACCAAGAA AGTCAAAAAG CCCCAAGTCG 
AAGAGATCAG AGAGCAAGGC TGAAGAACCC TCATTGGCTG AAGCACCTGA TAATCCCTTT 
ATCCGAGAGG GTTCTCTCAA GGCGTGGAAG GAGGAGCAGG ACCGACTGAA GGAGGAGGAG 
CGTCTCAGGG AGGAAAAGAA AGCTGTAAAG GGGAGGAAGT CAGCAATTAA GAAGAAAGGA 
GGTAGCAAGG AGCGGTCGGA GTCCCGGGGG AGCAAGGGGT CCCCAAAATC TCCTAGAAGC 
AGGAGAAAGA GCTCAAAAGA CAAAAGTACC GATGAGGAAA TAAAGTCACA GGAGTCAGTG 
ACTTTTGCAC CTGATCTTCC TGCGGCACCG GCACAAAAGG ACTTCAGGTT TCTTGGTTAC 
AACATGGGGG ACAATTTGAT CCAGGTCTCT GGGGGCAGCC GGCACCTCTT CCCCACAGAT 
GGGGGGCAGA TCCAGGCGGA ACATGTGCAC TTTGAGAAAG GCTCTTCCTT TGTGAAAGTG 
AAATTACTGA AGGACGGGCA CACGTTCCTG GTACATATCA CCAACCCCAC GAGACCCACC 
CAGGAAGAGC AGGAGGAGAC TGTCTGTGCT GATCAGGAAG GGAGACCAAA CATCACAAAG 
AAGAGCGTCA GTGAGTTTGG CTCCTTCTCA GCCACCCTGG AGAATGGCAT CCAGCTCTCT 
CTCAGTCACT ATGGCGCCTC GGGGAAAGGA CCAGAGGAGA AGGACCCGGC CCTGGCAGAA 
ATGCTGACCT TACCTAGTTT ACACACTCCG GGTTTAGTCC CAACACCTCC ACCTCCTGAA 
GCTTCTCCAC CTGCTGGAAA AGGCCGCAAA TCCCCCCGGC AGAAATCGGC CAAAGCTTCA 
AAGACTCCAC AGCCGCCACC GCAACCAGTT GAGGAGCCTC CTAAGCCTCC AGAGATAAAG 
GCAGAACCAG TAGAGCCCCC AGCCCAGCTT CCTTTAGAAC CAGCACCAGA AATTTCTACC 
TTCCAGAGTC TGAATGTCTC CTGTCCCAGT GGGCTCCTTG TGACATTCCT GCAGGAGCAG 
CCAACAGATC CGAAGGCTTC AGCTCCGGGG CCCAGGGAGA CACTGCTTGT GCGCCAGACT 
TACCCAGTGA GAGTGAGGAA CGCTCAACTC TACAAATCCA AAAATATTCT GGAGGCCTCC 
AGGGTCATCA CAGCCCAGGG CTCAGTCATT AAATACATGC TGGACGGCTC AACCAAGGTT 
CTGTTTCCTG ATGGGACAAT CAGCAGAAGT CCAGACTCTG GTCCCATTGT CCAGCCCCAG 
ACACCCCCGC CATCATCATC TGGAGAGGCT TCAGCACATG CCGAGACGGA GCCACCACCT 
CAGTCACTTC CCACGACCCC CCAGGAATCT AAAGAGGCAG CGGCAGAAGT TAGGAAAGGC 
AAAAGTGGCC AAAAAGTGCC AGTGGGCAAG ATGGAGATTC CGGAACCCTC TTTTCCCGAA 
ACTTCCCCTT TAACTTCAAG CGCCACTAAT GTCAAGCCAG CGGGCACCTG GATTACAACC 
ACCTCCTCTG GGCAACAGAT TGGTACCAGG GGCTCTGAGA GGCTGGAGCT GAACCCCCTT 
ATGTCCTACA TGGCTACCGA CCCTGTAAAT GGAACGGTGA TGATCACTCG AGAAGATCAG 
GTTGTTACTG TGCTCAAGCC CGATGGGACC AAGATCGCAG AACACGCAGA CGGTACCAGG 
ATCACAACAT TCTACCAGGA GACTGAGATG CCGGTACTGC GGGACCACAT GGAAACAGGG 
GAAGTCCCGC CCACTGTCAT AAAGAGGGTG GAGTTTGTAC GAGTTGAGAG CCCAGACTTC 
GCCACCGTTA TACTGAACCA GGAAGAGAAT ATCTGCTGCG CCGTGTTTGG GGATGGGACT 
GAGGTTCTGG CAACGCCTCA GGGGACCTAT CAGGTGCACC CCTCCATGTC TGGCACCCTG 
ACCATCAACC AGGATGGCGA CGCAGTCTAC TCACCTCACA CAAGCGCCTC CATTTTGGCC 
TCCCCCAGGC AGAAGGACCT GCCCCCAGGA AGTTACATCA TGAGTCACAC GGCGCCAGTG 
ATCTGTGAGG TTCTAGATCC AGAGGGGAAC CTCTTCCAGG TGCTGGTAGA TGGGAGCACA 
TCGGTGCATA TTGCCAGTGG GGAGAGCGGC GAGGAAGAGG AGGATGGTGA GAAAGGCCTT 
GCAGAGGCTG ATACCAGGGA ACCCAGCCCT GAGGTGTATG ATCTGCACGT GCCCAGGTTC 
TTCATTGTAA ATGCCGACGG CTCCGGGGGG GAGATTCTCC GCAATCGGGA AGTGGAAGAT 
TTCTTGGCTT CTTGTTACTC CGACCCTGCG ACTGCGGTGA TCCGGGAACC GGCGCCGGAA 
CTGCCAGGAG TCCAGTTCAT CACCGTCCTG AAGCCTCTCC CCGAAACGTC TCCGTGGGTC 
GTACAGAAAG AAACAAGTGA CCTGGTTCCC CCCAACCTCA TCTCCCGACC GTGGCACAAC 
TTTCCCCCTA TAGAGAGGGT ACGTGCGGGG CCGCCGTTGG GCATTGGAGT TTGGAAAGGG 
CTCAGTATTG GGGCTAAAGG GGAAGCCAGA TCTCACCCCC CAGCACTGAA ATGCCCCAAG 
GTTCTCCAGA TCCGCCAGCT CTCTTGCTAT GAGCCAATCA GTAAAGAGCA GCGCGAGAGG 
CTGCAGTTAT CTCTCAAGGG ATACATTGAT CATGTGCGCA AGAAGGAGGC GGAGCTAATG 
GAGGCAGCCA ATAAGGAACC CAGGACAGAG AATTCCACAG ACCTGCTAAA GCTGGTCCTG 
TCGTTTCCAG AATCCAGAGA ATCATCACAA ATATCAAGTT TGGACCAAGT TCAAGATGAC 
ATTGTTACCC TGTATGAGAG CGCAGTTGCC CCCAGCCCAG AGCCTCCTGC CCCCCCAGCC 
CAGCCGCACA GACATGGGGG AGACTGGCAA GAGATAAGGA GAGAAATCCA GGAGCAGAAG 
GAGACCCTGG AGGCAATGAG GGGCCGGGAC ATCCCCCCCT ACTTCCAGTC CGAGATGGGC 
AGGGCCTTCC TGGATACCCA GGGATACATT GATCATGTGC GCAAGAAGGA GGCGGAGCTA 
ATGGAGGCAG CCAATAAGGA ACCCAGGACA GAGAATTCCA CAGACCTGCT AAAGCTGGTC 
CTGTCGTTTC CAGAATCCAG AGAATCATCA CAAATATCAA GTTTGGACCA AGTTCAAGAT 
GACATTGTTA CCCTGTATGA GAGCGCAGTT GCCCCCAGCC CAGAGCCTCC TGCCCCCCCA 
GCCCAGCCGC ACAGACATGG GGGAGACTGG CAAGAGATAA GGAGAGAAAT CCAGGAGCAG 
AAGGAGACCC TGGAGGCAAT GAGGGGCCGG GACATCCCCC CCTACTTCCA GTCCGAGATG 
GGCAGGGCCT TCCTGGATAC CCAGGTGCCT GACATGGACC TGCTCTCCAG CCGGCTGCCC 
CCGTTCCCCC AGTCCCAGGA GAGAGAGGAG CAGCAGGAGT CCGGGTCCAG CGGTTCTGAT 
CCTGCAGCAG ACTTGAATCA TGGGGAGGGG CCCTCCAGCT CTGAGGAGTC CGATGAACCC 
ACCCGAAGCT CCAGCGCCCC CCGGAGAGAC ACAGAAGCAG CCAAGGAGCC CCCCCCATAC 
GTTCCAGAAA ATTCTGAGCC GAACTTTAAT TGGGCAGAGA TAATCCCGCC CAGGAACTAT 
TACCCAATGG CACGAAGTCT GAATTTGGAT GTAACTGGGA GGCCAAGGAA AGGGAAAGTA 
AAACTGCCGA ATTCCATCCT CAGTTCCAAA CCTGCCTCTG TGCCCAACAT TAAGCATGCA 
GTGGCAGAAG ATGCAGCGCG ACACAGAGGG AACATGGGTT CCGCCCCTTT GGGGGTTTGT 
ACCAGACACG TGCCGCGAGG TTTCCATCTC TCCCCGCCCG CTGTGCAGTT TGGGGTCCTG 
CGGGAAGGCT ACACCTACAC AGCGACAGTC ACAATAAAGA ATGTGGGGGT GGATCTCTGC 
AGGTTCCGAG TGAAGCAGCC GCCGCCCAGC ACTGGTTTGA GGGTGACGTA CACCCCGGGA 
CCGGTGGCGC CAGGGATGGA GAGCGACTTG GAGGTGGAGC TATTTGCTAT GGCGGTGGGA 
CTGGGGGGGC CGGAGGGGGC GGCAGAATGG TCTCACTGCA TAGAGATCCA CAGCGAGACT 
GAGATTCTCT TCCTCCCAGT CACTGGCACA GTTCTCACTG ACTCAGTGTA TGAGGCTGGA 
AGACTCCCCT GCGGGGGGAG GGCCCCCGGC ATCAGATTAG TCAGCACCGC CCCAAACTCC 
CGACTCCAGA TCCTGCGGCC CAGACGGACA CCCGACACAG TGTCATCCCT GGGATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123754.1
CDS	146..6985
Translation	MGPKRAKSTVPPAGSGPVGAPGVGSRSWESGLILAQLEQDDWRVSIAFVIENRPEDEAHTKALSQAVSVPLRQLFSVVSWADMIRQVQELGNPKVKKTKESPLYWEVTEVAKLLLDHGEKLPLSLIAKLLKFQFLNIKQKDLQRREAEKKAPEDKSKNKPTKAKSPGARSAGKSKGKKGPEAPPPVQKVTSLKRRGEEEETNRYIDDEPDDGAQHYIIVLGFYQPQLVALLAELGISVSCIIRISSQNYTSLPANEPDPTVAPEVLEAEQLRKVTLTKSLETFWKYLEPILNSGPPGSRLLEVARLHYMVKERHFPQDWGDSDLLLAYATELYENVACLMYDCLDWRRQHRRYLRNLRLIRVPSVSVGKSPDRQNVPPEAQEADPIPSSPTGRRKTQTDVAPPAASLPPSSPMHMEVEGPSGAAEVDTRFYTDLLSDLPEELQSVPVILHCVLEQVVATTEDLVPPSQAAPKPRPDGLDPNIASHMISVLDSLSLSDKEKKNLYNAFRAQEAEGERAEERRPELLNHHDKIGHRAAQAQPPSALDPVRIEQEMIGKLPLAQLLRFPQPAAERNSRRLAQIHELMHYCTSDLTPWEGVSWAFKLFTLESLPLTGLDDLGEVEGSGVEVQQDGDIPWDDPARFARTLGRMESVRRLHGPKAPEPEGNSSGQSQDQEETAGAEKEISPEEEAAQTQLGDIRRTQRRSLSDWCYWEHFNSELLKQVVCDAAQAYRCVDYYYHSQDNSLLLVMHNPMNPYHQSEESWDMALHSNVSFRNYLELVAESISNWVREEEEKYQEEMAVRELEALKQIQSRQDGEALPKANTKKSKRSPSPRKSKSPKSKRSESKAEEPSLAEAPDNPFIREGSLKAWKEEQDRLKEEERLREEKKAVKGRKSAIKKKGGSKERSESRGSKGSPKSPRSRRKSSKDKSTDEEIKSQESVTFAPDLPAAPAQKDFRFLGYNMGDNLIQVSGGSRHLFPTDGGQIQAEHVHFEKGSSFVKVKLLKDGHTFLVHITNPTRPTQEEQEETVCADQEGRPNITKKSVSEFGSFSATLENGIQLSLSHYGASGKGPEEKDPALAEMLTLPSLHTPGLVPTPPPPEASPPAGKGRKSPRQKSAKASKTPQPPPQPVEEPPKPPEIKAEPVEPPAQLPLEPAPEISTFQSLNVSCPSGLLVTFLQEQPTDPKASAPGPRETLLVRQTYPVRVRNAQLYKSKNILEASRVITAQGSVIKYMLDGSTKVLFPDGTISRSPDSGPIVQPQTPPPSSSGEASAHAETEPPPQSLPTTPQESKEAAAEVRKGKSGQKVPVGKMEIPEPSFPETSPLTSSATNVKPAGTWITTTSSGQQIGTRGSERLELNPLMSYMATDPVNGTVMITREDQVVTVLKPDGTKIAEHADGTRITTFYQETEMPVLRDHMETGEVPPTVIKRVEFVRVESPDFATVILNQEENICCAVFGDGTEVLATPQGTYQVHPSMSGTLTINQDGDAVYSPHTSASILASPRQKDLPPGSYIMSHTAPVICEVLDPEGNLFQVLVDGSTSVHIASGESGEEEEDGEKGLAEADTREPSPEVYDLHVPRFFIVNADGSGGEILRNREVEDFLASCYSDPATAVIREPAPELPGVQFITVLKPLPETSPWVVQKETSDLVPPNLISRPWHNFPPIERVRAGPPLGIGVWKGLSIGAKGEARSHPPALKCPKVLQIRQLSCYEPISKEQRERLQLSLKGYIDHVRKKEAELMEAANKEPRTENSTDLLKLVLSFPESRESSQISSLDQVQDDIVTLYESAVAPSPEPPAPPAQPHRHGGDWQEIRREIQEQKETLEAMRGRDIPPYFQSEMGRAFLDTQGYIDHVRKKEAELMEAANKEPRTENSTDLLKLVLSFPESRESSQISSLDQVQDDIVTLYESAVAPSPEPPAPPAQPHRHGGDWQEIRREIQEQKETLEAMRGRDIPPYFQSEMGRAFLDTQVPDMDLLSSRLPPFPQSQEREEQQESGSSGSDPAADLNHGEGPSSSEESDEPTRSSSAPRRDTEAAKEPPPYVPENSEPNFNWAEIIPPRNYYPMARSLNLDVTGRPRKGKVKLPNSILSSKPASVPNIKHAVAEDAARHRGNMGSAPLGVCTRHVPRGFHLSPPAVQFGVLREGYTYTATVTIKNVGVDLCRFRVKQPPPSTGLRVTYTPGPVAPGMESDLEVELFAMAVGLGGPEGAAEWSHCIEIHSETEILFLPVTGTVLTDSVYEAGRLPCGGRAPGIRLVSTAPNSRLQILRPRRTPDTVSSLGS

Target ORF information:

RefSeq Version	NM_001130282.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sperm associated antigen 17 (spag17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130282.1

ORF Insert Sequence:

ATGGGCCCGA AAAGAGCGAA GAGCACCGTC CCCCCGGCGG GTAGTGGCCC TGTGGGGGCC 
CCGGGTGTGG GCTCTCGGAG CTGGGAGTCC GGCCTTATCC TGGCGCAGCT GGAGCAGGAT 
GACTGGAGGG TCAGCATCGC CTTTGTCATT GAGAACCGCC CCGAGGATGA AGCCCACACG 
AAGGCGCTGT CCCAGGCTGT GTCCGTGCCG CTGCGCCAGC TCTTCAGTGT GGTGTCGTGG 
GCCGACATGA TCCGACAGGT CCAGGAGCTG GGAAATCCGA AGGTCAAGAA AACAAAGGAG 
TCGCCATTAT ATTGGGAGGT CACTGAGGTG GCCAAACTGC TGTTGGATCA CGGAGAAAAA 
CTGCCCCTGT CCCTGATTGC AAAACTGCTG AAATTCCAGT TCCTGAACAT CAAGCAGAAA 
GACCTGCAGA GGAGGGAGGC CGAGAAGAAG GCCCCAGAAG ACAAGTCGAA GAACAAACCC 
ACAAAGGCCA AATCACCCGG TGCCCGGTCT GCCGGCAAAT CAAAAGGAAA AAAAGGCCCA 
GAGGCTCCAC CCCCTGTCCA AAAAGTTACC TCCCTGAAGC GCCGGGGGGA AGAAGAAGAA 
ACCAACAGAT ATATAGATGA TGAGCCGGAC GATGGTGCCC AACATTACAT CATTGTCCTT 
GGCTTCTATC AGCCTCAGCT CGTGGCCCTG TTGGCCGAAC TAGGGATCTC CGTGTCCTGT 
ATTATAAGGA TATCGTCCCA GAACTACACG AGCCTTCCTG CCAATGAGCC GGATCCAACA 
GTGGCCCCTG AAGTTCTAGA GGCAGAACAG CTCAGGAAAG TGACTCTCAC AAAATCCTTG 
GAAACGTTCT GGAAGTACCT GGAGCCCATT CTGAACAGCG GCCCCCCGGG ATCCCGACTT 
CTGGAAGTGG CTCGGCTGCA CTATATGGTC AAGGAAAGGC ACTTTCCTCA GGACTGGGGT 
GACAGTGACC TCCTGTTGGC ATATGCCACA GAGCTGTATG AGAACGTTGC CTGCCTCATG 
TACGACTGTC TGGACTGGAG GCGGCAGCAC CGGCGCTACC TGCGTAACCT GCGGCTCATC 
CGCGTCCCCT CTGTGTCCGT AGGGAAGAGC CCAGACAGAC AGAACGTCCC GCCCGAGGCC 
CAGGAAGCTG ACCCCATCCC ATCATCCCCT ACAGGCAGGA GGAAAACTCA GACTGATGTG 
GCGCCTCCAG CTGCTTCTCT GCCACCGTCA TCTCCAATGC ACATGGAGGT CGAGGGCCCG 
AGTGGGGCGG CTGAGGTGGA TACGAGATTT TACACTGATC TCCTGAGCGA TCTCCCAGAG 
GAGCTTCAGT CGGTCCCAGT GATACTGCAC TGTGTGCTGG AACAGGTTGT GGCCACTACG 
GAGGATCTTG TGCCCCCCAG CCAAGCTGCA CCCAAGCCCC GCCCAGATGG GCTGGACCCC 
AATATTGCCA GTCACATGAT ATCTGTGTTG GATTCTCTCT CCCTGTCGGA CAAGGAAAAG 
AAGAACCTGT ACAACGCGTT CCGTGCCCAA GAGGCAGAGG GTGAGCGGGC AGAGGAGAGA 
CGGCCGGAGC TGCTGAACCA CCATGATAAG ATTGGGCACA GGGCCGCCCA GGCACAGCCC 
CCCAGCGCAT TGGATCCAGT CAGAATAGAG CAGGAAATGA TTGGAAAACT TCCCCTTGCG 
CAACTTCTGC GATTTCCCCA ACCTGCAGCG GAGAGGAACT CACGGAGACT CGCGCAGATA 
CATGAACTCA TGCACTACTG CACGTCTGAC CTGACACCCT GGGAAGGAGT CTCGTGGGCA 
TTCAAGCTGT TCACGTTGGA GAGCCTCCCG CTGACTGGAT TGGATGACCT GGGGGAAGTG 
GAGGGCAGTG GGGTGGAGGT GCAGCAAGAT GGCGACATTC CGTGGGACGA TCCCGCCAGA 
TTCGCTCGCA CACTGGGCAG GATGGAAAGT GTGAGGCGGC TGCACGGGCC CAAAGCGCCA 
GAACCTGAAG GGAATTCTTC CGGGCAAAGT CAAGATCAGG AGGAAACGGC AGGAGCAGAA 
AAGGAGATTT CCCCAGAGGA GGAGGCCGCA CAGACACAAC TGGGAGACAT TAGGAGGACA 
CAGAGAAGAT CTCTGAGTGA CTGGTGCTAC TGGGAGCATT TTAATTCAGA ACTACTCAAG 
CAGGTGGTGT GTGACGCCGC TCAGGCCTAC AGGTGTGTGG ATTACTATTA TCACTCTCAG 
GATAACTCTC TGCTCCTCGT TATGCACAAT CCCATGAACC CCTACCATCA GAGCGAGGAA 
TCCTGGGACA TGGCTCTCCA TTCCAACGTC AGTTTCAGAA ACTACCTGGA GTTGGTAGCA 
GAGTCCATTT CCAACTGGGT GAGGGAGGAG GAGGAGAAGT ATCAGGAGGA GATGGCAGTC 
AGAGAGTTGG AAGCTTTGAA ACAAATTCAA TCTAGGCAGG ATGGTGAGGC CCTACCAAAA 
GCCAACACCA AGAAATCCAA GAGATCCCCC TCACCAAGAA AGTCAAAAAG CCCCAAGTCG 
AAGAGATCAG AGAGCAAGGC TGAAGAACCC TCATTGGCTG AAGCACCTGA TAATCCCTTT 
ATCCGAGAGG GTTCTCTCAA GGCGTGGAAG GAGGAGCAGG ACCGACTGAA GGAGGAGGAG 
CGTCTCAGGG AGGAAAAGAA AGCTGTAAAG GGGAGGAAGT CAGCAATTAA GAAGAAAGGA 
GGTAGCAAGG AGCGGTCGGA GTCCCGGGGG AGCAAGGGGT CCCCAAAATC TCCTAGAAGC 
AGGAGAAAGA GCTCAAAAGA CAAAAGTACC GATGAGGAAA TAAAGTCACA GGAGTCAGTG 
ACTTTTGCAC CTGATCTTCC TGCGGCACCG GCACAAAAGG ACTTCAGGTT TCTTGGTTAC 
AACATGGGGG ACAATTTGAT CCAGGTCTCT GGGGGCAGCC GGCACCTCTT CCCCACAGAT 
GGGGGGCAGA TCCAGGCGGA ACATGTGCAC TTTGAGAAAG GCTCTTCCTT TGTGAAAGTG 
AAATTACTGA AGGACGGGCA CACGTTCCTG GTACATATCA CCAACCCCAC GAGACCCACC 
CAGGAAGAGC AGGAGGAGAC TGTCTGTGCT GATCAGGAAG GGAGACCAAA CATCACAAAG 
AAGAGCGTCA GTGAGTTTGG CTCCTTCTCA GCCACCCTGG AGAATGGCAT CCAGCTCTCT 
CTCAGTCACT ATGGCGCCTC GGGGAAAGGA CCAGAGGAGA AGGACCCGGC CCTGGCAGAA 
ATGCTGACCT TACCTAGTTT ACACACTCCG GGTTTAGTCC CAACACCTCC ACCTCCTGAA 
GCTTCTCCAC CTGCTGGAAA AGGCCGCAAA TCCCCCCGGC AGAAATCGGC CAAAGCTTCA 
AAGACTCCAC AGCCGCCACC GCAACCAGTT GAGGAGCCTC CTAAGCCTCC AGAGATAAAG 
GCAGAACCAG TAGAGCCCCC AGCCCAGCTT CCTTTAGAAC CAGCACCAGA AATTTCTACC 
TTCCAGAGTC TGAATGTCTC CTGTCCCAGT GGGCTCCTTG TGACATTCCT GCAGGAGCAG 
CCAACAGATC CGAAGGCTTC AGCTCCGGGG CCCAGGGAGA CACTGCTTGT GCGCCAGACT 
TACCCAGTGA GAGTGAGGAA CGCTCAACTC TACAAATCCA AAAATATTCT GGAGGCCTCC 
AGGGTCATCA CAGCCCAGGG CTCAGTCATT AAATACATGC TGGACGGCTC AACCAAGGTT 
CTGTTTCCTG ATGGGACAAT CAGCAGAAGT CCAGACTCTG GTCCCATTGT CCAGCCCCAG 
ACACCCCCGC CATCATCATC TGGAGAGGCT TCAGCACATG CCGAGACGGA GCCACCACCT 
CAGTCACTTC CCACGACCCC CCAGGAATCT AAAGAGGCAG CGGCAGAAGT TAGGAAAGGC 
AAAAGTGGCC AAAAAGTGCC AGTGGGCAAG ATGGAGATTC CGGAACCCTC TTTTCCCGAA 
ACTTCCCCTT TAACTTCAAG CGCCACTAAT GTCAAGCCAG CGGGCACCTG GATTACAACC 
ACCTCCTCTG GGCAACAGAT TGGTACCAGG GGCTCTGAGA GGCTGGAGCT GAACCCCCTT 
ATGTCCTACA TGGCTACCGA CCCTGTAAAT GGAACGGTGA TGATCACTCG AGAAGATCAG 
GTTGTTACTG TGCTCAAGCC CGATGGGACC AAGATCGCAG AACACGCAGA CGGTACCAGG 
ATCACAACAT TCTACCAGGA GACTGAGATG CCGGTACTGC GGGACCACAT GGAAACAGGG 
GAAGTCCCGC CCACTGTCAT AAAGAGGGTG GAGTTTGTAC GAGTTGAGAG CCCAGACTTC 
GCCACCGTTA TACTGAACCA GGAAGAGAAT ATCTGCTGCG CCGTGTTTGG GGATGGGACT 
GAGGTTCTGG CAACGCCTCA GGGGACCTAT CAGGTGCACC CCTCCATGTC TGGCACCCTG 
ACCATCAACC AGGATGGCGA CGCAGTCTAC TCACCTCACA CAAGCGCCTC CATTTTGGCC 
TCCCCCAGGC AGAAGGACCT GCCCCCAGGA AGTTACATCA TGAGTCACAC GGCGCCAGTG 
ATCTGTGAGG TTCTAGATCC AGAGGGGAAC CTCTTCCAGG TGCTGGTAGA TGGGAGCACA 
TCGGTGCATA TTGCCAGTGG GGAGAGCGGC GAGGAAGAGG AGGATGGTGA GAAAGGCCTT 
GCAGAGGCTG ATACCAGGGA ACCCAGCCCT GAGGTGTATG ATCTGCACGT GCCCAGGTTC 
TTCATTGTAA ATGCCGACGG CTCCGGGGGG GAGATTCTCC GCAATCGGGA AGTGGAAGAT 
TTCTTGGCTT CTTGTTACTC CGACCCTGCG ACTGCGGTGA TCCGGGAACC GGCGCCGGAA 
CTGCCAGGAG TCCAGTTCAT CACCGTCCTG AAGCCTCTCC CCGAAACGTC TCCGTGGGTC 
GTACAGAAAG AAACAAGTGA CCTGGTTCCC CCCAACCTCA TCTCCCGACC GTGGCACAAC 
TTTCCCCCTA TAGAGAGGGT ACGTGCGGGG CCGCCGTTGG GCATTGGAGT TTGGAAAGGG 
CTCAGTATTG GGGCTAAAGG GGAAGCCAGA TCTCACCCCC CAGCACTGAA ATGCCCCAAG 
GTTCTCCAGA TCCGCCAGCT CTCTTGCTAT GAGCCAATCA GTAAAGAGCA GCGCGAGAGG 
CTGCAGTTAT CTCTCAAGGG ATACATTGAT CATGTGCGCA AGAAGGAGGC GGAGCTAATG 
GAGGCAGCCA ATAAGGAACC CAGGACAGAG AATTCCACAG ACCTGCTAAA GCTGGTCCTG 
TCGTTTCCAG AATCCAGAGA ATCATCACAA ATATCAAGTT TGGACCAAGT TCAAGATGAC 
ATTGTTACCC TGTATGAGAG CGCAGTTGCC CCCAGCCCAG AGCCTCCTGC CCCCCCAGCC 
CAGCCGCACA GACATGGGGG AGACTGGCAA GAGATAAGGA GAGAAATCCA GGAGCAGAAG 
GAGACCCTGG AGGCAATGAG GGGCCGGGAC ATCCCCCCCT ACTTCCAGTC CGAGATGGGC 
AGGGCCTTCC TGGATACCCA GGGATACATT GATCATGTGC GCAAGAAGGA GGCGGAGCTA 
ATGGAGGCAG CCAATAAGGA ACCCAGGACA GAGAATTCCA CAGACCTGCT AAAGCTGGTC 
CTGTCGTTTC CAGAATCCAG AGAATCATCA CAAATATCAA GTTTGGACCA AGTTCAAGAT 
GACATTGTTA CCCTGTATGA GAGCGCAGTT GCCCCCAGCC CAGAGCCTCC TGCCCCCCCA 
GCCCAGCCGC ACAGACATGG GGGAGACTGG CAAGAGATAA GGAGAGAAAT CCAGGAGCAG 
AAGGAGACCC TGGAGGCAAT GAGGGGCCGG GACATCCCCC CCTACTTCCA GTCCGAGATG 
GGCAGGGCCT TCCTGGATAC CCAGGTGCCT GACATGGACC TGCTCTCCAG CCGGCTGCCC 
CCGTTCCCCC AGTCCCAGGA GAGAGAGGAG CAGCAGGAGT CCGGGTCCAG CGGTTCTGAT 
CCTGCAGCAG ACTTGAATCA TGGGGAGGGG CCCTCCAGCT CTGAGGAGTC CGATGAACCC 
ACCCGAAGCT CCAGCGCCCC CCGGAGAGAC ACAGAAGCAG CCAAGGAGCC CCCCCCATAC 
GTTCCAGAAA ATTCTGAGCC GAACTTTAAT TGGGCAGAGA TAATCCCGCC CAGGAACTAT 
TACCCAATGG CACGAAGTCT GAATTTGGAT GTAACTGGGA GGCCAAGGAA AGGGAAAGTA 
AAACTGCCGA ATTCCATCCT CAGTTCCAAA CCTGCCTCTG TGCCCAACAT TAAGCATGCA 
GTGGCAGAAG ATGCAGCGCG ACACAGAGGG AACATGGGTT CCGCCCCTTT GGGGGTTTGT 
ACCAGACACG TGCCGCGAGG TTTCCATCTC TCCCCGCCCG CTGTGCAGTT TGGGGTCCTG 
CGGGAAGGCT ACACCTACAC AGCGACAGTC ACAATAAAGA ATGTGGGGGT GGATCTCTGC 
AGGTTCCGAG TGAAGCAGCC GCCGCCCAGC ACTGGTTTGA GGGTGACGTA CACCCCGGGA 
CCGGTGGCGC CAGGGATGGA GAGCGACTTG GAGGTGGAGC TATTTGCTAT GGCGGTGGGA 
CTGGGGGGGC CGGAGGGGGC GGCAGAATGG TCTCACTGCA TAGAGATCCA CAGCGAGACT 
GAGATTCTCT TCCTCCCAGT CACTGGCACA GTTCTCACTG ACTCAGTGTA TGAGGCTGGA 
AGACTCCCCT GCGGGGGGAG GGCCCCCGGC ATCAGATTAG TCAGCACCGC CCCAAACTCC 
CGACTCCAGA TCCTGCGGCC CAGACGGACA CCCGACACAG TGTCATCCCT GGGATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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spag17 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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