fmn1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	fmn1
Entrez Gene ID	100170173
Full Name	formin 1
Synonyms	fmn,formin
General protein information	Preferred Names formin 1 Names formin-1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012968078.2	XP_012823532.1	formin-1 isoform X1
XM_031890237.1	XP_031746097.1	formin-1 isoform X1
XM_031890238.1	XP_031746098.1	formin-1 isoform X2
XM_012968078.3	XP_012823532.2	formin-1 isoform X3
XM_012968079.3	XP_012823533.2	formin-1 isoform X4
NM_001129929.2	NP_001123401.2	formin-1
XM_012968079.2	XP_012823533.1	formin-1 isoform X2

Pathways from BioSystems

Homologies

	FMN1	XP_001084432.2
Homo sapiens (human)	FMN1	NP_001264242.1
Pan troglodytes (chimpanzee)	FMN1	XP_003314633.1
Xenopus tropicalis (tropical clawed frog)	fmn1	NP_001123401.2
Mus musculus (house mouse)	Fmn1	NP_001272387.1
Rattus norvegicus (Norway rat)	Fmn1	XP_006234779.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following fmn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fmn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa63521	XM_031890237.1 Latest version!	Xenopus tropicalis formin 1 (fmn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa63522	XM_031890238.1 Latest version!	Xenopus tropicalis formin 1 (fmn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa63523	XM_012968078.3 Latest version!	Xenopus tropicalis formin 1 (fmn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63524	XM_012968079.3 Latest version!	Xenopus tropicalis formin 1 (fmn1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa25292	NM_001129929.2 Latest version!	Xenopus tropicalis formin 1 (fmn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OXa32276	XM_012968078.2 Latest version!	Xenopus tropicalis formin 1 (fmn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OXa32277	XM_012968079.2 Latest version!	Xenopus tropicalis formin 1 (fmn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fmn1 ( XM_031890237.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746097.1 Xenopus tropicalis formin 1 (fmn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63521
Clone ID Related Accession (Same CDS sequence)	XM_031890237.1
Accession Version	XM_031890237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	formin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCA CACACTCCAT CCTCCAATTG CACAAACCCA TTATGGAGCT CTGCTACGTC 
AGCTTCTTCC TTCCCAGAGG TAATGTCAGG GGATTCACAT ACAAACGCTG TGTAACTCGA 
GACAAAGCCA GAACATGTTT TGATAACTGT TATCGAATCA GCGAGGAGAG AGAGACTGCA 
GCACATAAAG ATCCACCTTA TGAAAGATTT ACAGAGGACC TGAAGGAAGC CACAACCCAA 
AACATACTGA CAGAGCTCTA CAAACTCACA GCTGCTAAAG ACAGACTTTT AGTTCAGCTC 
CTAGAAGCCT CCCATAAGCT AGGATCTATA ATGGGAAACC AGGATGGCAA ATTTCAGGAT 
CTTCCGGACG GATATAAATT TCCAGAAGAC GAGGCCCATG GCCATGTGGA TTTCCATGAG 
TCATTTTCAG CTTCAGTGGA GAAGAAGGTG CCTTCCACAA AGGTAAAGAA GAATAGAAGG 
CTTAGTCGGA GGAAAGAAAG TATAGAAGAT TTTGTGAACA AGAAGATAAA GTGGAAAAGT 
AGCACAGAAG CTTCTGCAAC ACAGGTTAGA GACAAAACGT CCTCAGCAAA GGTTTCCCTG 
ACGGGCTCTA CGGATTCATT GTCACGCCCA AAACTGCAGT CTGTGGAGAA CAAGGATAGT 
TACTTACCAG GGCCCATGGT GAAAAATGCA CAGGGTAAGC ACAGTGAAAG CTCAGGGATG 
CTGGGTTCTG AGTCTGCAAT GGGAAGCTTA TTGTCTGTGT ATGATAATGA TGTTTTTGCT 
GATTTTTCTC TGCTGCCTCA CAGTGACAGT CTTTTAAAAG AACTTCAGAG TGCAATTGAC 
ATTATGCCAG AGCAACCAAG CACCCAAGTA CTGGAAGGTT ATATATTTGA AAAGCATTGC 
ACTGAGCATG AGAGCAATGA GCAGGCATCC AGTGGGAGAA GTATAAAGAC AGTTACTGCT 
AAATTGCAGG ACATTAGCAC TTGTGTACAG AAAGTGGTTC ATACTTATAA CTATCAAGAA 
GACGGAGCTG AAGAAAGGAG GTGTGAAAAT ATTACTGAGC TCATTGATGA ACAGAAGGAG 
TTTGTATCAC AGATTGATGT ACCAATGATG TATGAACCAT TACAGTGTCA TATAGAAAAG 
CAGCTATATG GGGGTGTTTA TAATGACAGA CACGGTAGAC TAGAGACATG CAACAATAAT 
ATAGAGCCGC TGCCTTCCTG TGAATATGTC AACAAAAACC TTCTCAGGGT TGTCAAAAGT 
GAAAGCATGG ACGAAACAGA GTATTGGCTG GGACAGATGG ATAGGAGAGG AGAGAAGTGC 
AGTTATGGCC TTAAGCTGCC AGAGAAACTA AGTAAGTCAC AAGAATCATT AAACCATGTA 
ACTGTGCCAC ATAGTGCTCA TATTAAGTTA CAAGACAAAC CTACTGCAGT GAAAACACCA 
ACCTTTGATA CGGCCAGCAT CATGCATATA CCATGTACTA TGTGCAAAGG GATTTCCCCT 
GTTTCTTCTC CAGTATCTAC GCACCTGTCA AGCCCACAGC CCCATCATAG GATTCTCCCC 
TTGCAAACTC AACATGTAGA GGAAGATCTA AAGTTGTGTG GTGTTGAACA TTGGTCTGCC 
CTACCTGATA TTTTCAGAGA ACCATCATCA CTCCAAGAGC TGGGGTATGA TTTTAAAGGA 
TCTACCACGT TCAACATGGA CCCTGATAAC TATCTTTTTG ATGATTTCTT GCAAAATGTG 
AAAGGAAAAA AGACCACATT ACAGCCTAAT CTGCCTCCTC CAGGAGGTGG TGAAGCCTTG 
AAAGATGTTT CCAGAGGTCC AGATACTTCT TCTGGTCTCC AGGCCGGTGT CAGTGAGGAG 
CTGTCAAGTG TTTGTGATAA TAAAACAACT GTCGGGAGGC CCGACCACAG TATTTTGCAT 
CTCAGTCTGG AAATACATAC CGACCTCAGT CCTTCTGATG GAGAAGATCG AACCCCAGGA 
AGACTCCAAG CAGTGTGGCC ACCCCCAAAG CCCAAAGATG AAGAAGAAAA AGTGGGTCTG 
AAATACACAG AAGCAGAGTA TCAGGCTGCT ATCCTGCATT TGAAGAGGGA ACACAAAGAT 
GAGCTAGAGA CACTGAAGTC CCAATCTGAA GTGGCGATCT TCAATGTGCG AGGGGAACAA 
GCAGTTCAGA CAGCAAAATT AGAGGAGGAA ATACAGAAAC TGCAAGCGGA CCTACAGAAT 
AAACTCAGTC ACCAGAATGG GGTGAAAAGG GATGTTTGTG TTTCCACCGA AGATGAGAAT 
TCTCCAAAAT CTTTTCGAAA TGTATGCATC CAAACTGACC GTGAGACTTT TTTGAAACCC 
AATGAGGAGG ACAAATCTCA AAAAAATGAC CCTGTTTTAC CAAAGAAATT AAATATTGCA 
TCAATCAGTC AAAACCTCGC TAGTCCCATA AGTCCCAGCG ATAATTCACA GCACAATTTC 
CCTTTAGCTT CCTTGTCAGT TACTACACCA CCTCCCCACC CCCTCTCTAC ATCTTCACAA 
ACAGCCTCTT CTCCAACACC CTTAGATTGC ACTTCTATTC CTCCACCTCC ATTACTTCCA 
GGTTTCCCTT CTGTTCCACC ACCTCCACCT CTTCCTGGTT CTAGCTCTGT TCCACCTCCT 
CCACCTCTTC CAGGTATCAG TTCTGCCCCA CCACCTCCCC CTCTTCCAGG TTTTAGCTCT 
GTTCCACCTC CTCCTCCTCC TCTTCCAGAT TTAAGTTCTG TTCCACCCCC TCCCCCTTTT 
CCAGGTGGGG GTCCTCCTCC ACCACCTCCT CCTTTTCCTG GGTATGGAAG CTCAGCAGTT 
CCTCCACCAC TACCTCTACC ACTCCCCGGG CTTTTTTTTG GGCTGTCAAA ACCTCGAAAA 
GCCCCTGTAG AGCCTGGCTG CCCAATGAAG CCGCTGTATT GGACAAGGAT TCAACTAAAA 
AATGGCAGCT CCAGCAATAC GTTATGGGAG AACCTAAAGG AGCCCAACAT TGCAGACACT 
AAAGAATTTG AGGACCTATT TGCAAAAGCC ACTGTTCAAC AGAAGAAGAA ACCACTGTCA 
GACTCTTATG AGAAGAGAGC AAAAGCTAAG CAGGTTATAA AGCTACTGGA TGGGAAGAGG 
TCTCAGGCTG TGGGCATTTT AATATCCAGT TTACATCTGG ACATGAAGGA TATTCAACAA 
GCAATTCTTA ATGTAGATAA TTCAGTTGTG GACATAGAAA CTCTTGAAGC CTTGTATGAA 
AACAGGGCTC AGAAGGAGGA GATGGAAATC ATCAAAAAAC ACTATCAGAC ATCTAAAGCT 
GAGGATGTAA AACTGCTGGA CAAACCTGAA CAGTTTCTTT ACGAGCTGTC TCAGATTCCC 
AACTTTGTTG AGCGATCTCA GTGTATTATA TTCCAGTCTG TCTTTTTAGA GGGAATAAGC 
TCAGTGAGGC GGAAGGTGGA TATCATCAGT CGGGCCTGCG ATTGCCTCTT GGAGAGGGTT 
TCTGTGAGAG ATATAATAGG TCTGATTCTT GCATTCGGAA ACTACATGAA TGGAGGCAAC 
CGTACTCGGG GTCAGGCAGA CGGATTTGGG CTGGAAATCC TTCCCAAACT AAAGGATGTT 
AAAAGCAGGG ATAATAAAGC CAGCCTGGTG GATTATGTTG TACGTTATTA TCTACGATAT 
TTTGACCAGG ATGCTGGAAC AGACAAAAGT GTTTTTCCAC TGCCAGAATC CCAGGACCTT 
TTACTGGCTT CGCAGGTGAA ATTTGAAGAC CTGGACAAAG ACTTACGGAA ACTTAAAAAG 
GACATTGAAG TGTGTGAGAA ACAGTTGAAA GCAGTGGTAA AAGACTCGCC TAAAGAGAAT 
ATCCAGCCAT TTAAGGACAA GATGTCATCT TTTTTAAACA AAGCCAAAGA AGAATATAAG 
ACAGAAGAAA GTAAACTGAC CAGATCACAA AACAGGTTTG AAGAAACAAT TGGATATTTT 
GGATGTAAGC CAAAGGGCAA TGATAAAGAG ATCACCCCCA ACTCCTTCTT TGTCTTGTGG 
TACGAATTCT GCGGTGACTT CAAGAGTGTG TGGAAAAGAG AGAGCAAAGT CCTCTCCACG 
GAAAGGCGCC TTTGTTTTCT TTTCGGATCA AATGGACATC AAGGGAAGGT GATTCCCCCC 
CAGGGCTCCA GAGGGAACCC GCTAGCGGCC GTCTGTCCTC ACGAAAAGAT AAACACGCCA 
GATCTGATAA GTTACACTTT TCTTTACCAC TCCCAATTAC TTGCTAGTAA AATGAAAGGA 
GACCTCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746097.1
CDS	326..4597
Translation	MEGTHSILQLHKPIMELCYVSFFLPRGNVRGFTYKRCVTRDKARTCFDNCYRISEERETAAHKDPPYERFTEDLKEATTQNILTELYKLTAAKDRLLVQLLEASHKLGSIMGNQDGKFQDLPDGYKFPEDEAHGHVDFHESFSASVEKKVPSTKVKKNRRLSRRKESIEDFVNKKIKWKSSTEASATQVRDKTSSAKVSLTGSTDSLSRPKLQSVENKDSYLPGPMVKNAQGKHSESSGMLGSESAMGSLLSVYDNDVFADFSLLPHSDSLLKELQSAIDIMPEQPSTQVLEGYIFEKHCTEHESNEQASSGRSIKTVTAKLQDISTCVQKVVHTYNYQEDGAEERRCENITELIDEQKEFVSQIDVPMMYEPLQCHIEKQLYGGVYNDRHGRLETCNNNIEPLPSCEYVNKNLLRVVKSESMDETEYWLGQMDRRGEKCSYGLKLPEKLSKSQESLNHVTVPHSAHIKLQDKPTAVKTPTFDTASIMHIPCTMCKGISPVSSPVSTHLSSPQPHHRILPLQTQHVEEDLKLCGVEHWSALPDIFREPSSLQELGYDFKGSTTFNMDPDNYLFDDFLQNVKGKKTTLQPNLPPPGGGEALKDVSRGPDTSSGLQAGVSEELSSVCDNKTTVGRPDHSILHLSLEIHTDLSPSDGEDRTPGRLQAVWPPPKPKDEEEKVGLKYTEAEYQAAILHLKREHKDELETLKSQSEVAIFNVRGEQAVQTAKLEEEIQKLQADLQNKLSHQNGVKRDVCVSTEDENSPKSFRNVCIQTDRETFLKPNEEDKSQKNDPVLPKKLNIASISQNLASPISPSDNSQHNFPLASLSVTTPPPHPLSTSSQTASSPTPLDCTSIPPPPLLPGFPSVPPPPPLPGSSSVPPPPPLPGISSAPPPPPLPGFSSVPPPPPPLPDLSSVPPPPPFPGGGPPPPPPPFPGYGSSAVPPPLPLPLPGLFFGLSKPRKAPVEPGCPMKPLYWTRIQLKNGSSSNTLWENLKEPNIADTKEFEDLFAKATVQQKKKPLSDSYEKRAKAKQVIKLLDGKRSQAVGILISSLHLDMKDIQQAILNVDNSVVDIETLEALYENRAQKEEMEIIKKHYQTSKAEDVKLLDKPEQFLYELSQIPNFVERSQCIIFQSVFLEGISSVRRKVDIISRACDCLLERVSVRDIIGLILAFGNYMNGGNRTRGQADGFGLEILPKLKDVKSRDNKASLVDYVVRYYLRYFDQDAGTDKSVFPLPESQDLLLASQVKFEDLDKDLRKLKKDIEVCEKQLKAVVKDSPKENIQPFKDKMSSFLNKAKEEYKTEESKLTRSQNRFEETIGYFGCKPKGNDKEITPNSFFVLWYEFCGDFKSVWKRESKVLSTERRLCFLFGSNGHQGKVIPPQGSRGNPLAAVCPHEKINTPDLISYTFLYHSQLLASKMKGDLS

Target ORF information:

RefSeq Version	XM_031890237.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis formin 1 (fmn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890237.1

ORF Insert Sequence:

ATGGAAGGCA CACACTCCAT CCTCCAATTG CACAAACCCA TTATGGAGCT CTGCTACGTC 
AGCTTCTTCC TTCCCAGAGG TAATGTCAGG GGATTCACAT ACAAACGCTG TGTAACTCGA 
GACAAAGCCA GAACATGTTT TGATAACTGT TATCGAATCA GCGAGGAGAG AGAGACTGCA 
GCACATAAAG ATCCACCTTA TGAAAGATTT ACAGAGGACC TGAAGGAAGC CACAACCCAA 
AACATACTGA CAGAGCTCTA CAAACTCACA GCTGCTAAAG ACAGACTTTT AGTTCAGCTC 
CTAGAAGCCT CCCATAAGCT AGGATCTATA ATGGGAAACC AGGATGGCAA ATTTCAGGAT 
CTTCCGGACG GATATAAATT TCCAGAAGAC GAGGCCCATG GCCATGTGGA TTTCCATGAG 
TCATTTTCAG CTTCAGTGGA GAAGAAGGTG CCTTCCACAA AGGTAAAGAA GAATAGAAGG 
CTTAGTCGGA GGAAAGAAAG TATAGAAGAT TTTGTGAACA AGAAGATAAA GTGGAAAAGT 
AGCACAGAAG CTTCTGCAAC ACAGGTTAGA GACAAAACGT CCTCAGCAAA GGTTTCCCTG 
ACGGGCTCTA CGGATTCATT GTCACGCCCA AAACTGCAGT CTGTGGAGAA CAAGGATAGT 
TACTTACCAG GGCCCATGGT GAAAAATGCA CAGGGTAAGC ACAGTGAAAG CTCAGGGATG 
CTGGGTTCTG AGTCTGCAAT GGGAAGCTTA TTGTCTGTGT ATGATAATGA TGTTTTTGCT 
GATTTTTCTC TGCTGCCTCA CAGTGACAGT CTTTTAAAAG AACTTCAGAG TGCAATTGAC 
ATTATGCCAG AGCAACCAAG CACCCAAGTA CTGGAAGGTT ATATATTTGA AAAGCATTGC 
ACTGAGCATG AGAGCAATGA GCAGGCATCC AGTGGGAGAA GTATAAAGAC AGTTACTGCT 
AAATTGCAGG ACATTAGCAC TTGTGTACAG AAAGTGGTTC ATACTTATAA CTATCAAGAA 
GACGGAGCTG AAGAAAGGAG GTGTGAAAAT ATTACTGAGC TCATTGATGA ACAGAAGGAG 
TTTGTATCAC AGATTGATGT ACCAATGATG TATGAACCAT TACAGTGTCA TATAGAAAAG 
CAGCTATATG GGGGTGTTTA TAATGACAGA CACGGTAGAC TAGAGACATG CAACAATAAT 
ATAGAGCCGC TGCCTTCCTG TGAATATGTC AACAAAAACC TTCTCAGGGT TGTCAAAAGT 
GAAAGCATGG ACGAAACAGA GTATTGGCTG GGACAGATGG ATAGGAGAGG AGAGAAGTGC 
AGTTATGGCC TTAAGCTGCC AGAGAAACTA AGTAAGTCAC AAGAATCATT AAACCATGTA 
ACTGTGCCAC ATAGTGCTCA TATTAAGTTA CAAGACAAAC CTACTGCAGT GAAAACACCA 
ACCTTTGATA CGGCCAGCAT CATGCATATA CCATGTACTA TGTGCAAAGG GATTTCCCCT 
GTTTCTTCTC CAGTATCTAC GCACCTGTCA AGCCCACAGC CCCATCATAG GATTCTCCCC 
TTGCAAACTC AACATGTAGA GGAAGATCTA AAGTTGTGTG GTGTTGAACA TTGGTCTGCC 
CTACCTGATA TTTTCAGAGA ACCATCATCA CTCCAAGAGC TGGGGTATGA TTTTAAAGGA 
TCTACCACGT TCAACATGGA CCCTGATAAC TATCTTTTTG ATGATTTCTT GCAAAATGTG 
AAAGGAAAAA AGACCACATT ACAGCCTAAT CTGCCTCCTC CAGGAGGTGG TGAAGCCTTG 
AAAGATGTTT CCAGAGGTCC AGATACTTCT TCTGGTCTCC AGGCCGGTGT CAGTGAGGAG 
CTGTCAAGTG TTTGTGATAA TAAAACAACT GTCGGGAGGC CCGACCACAG TATTTTGCAT 
CTCAGTCTGG AAATACATAC CGACCTCAGT CCTTCTGATG GAGAAGATCG AACCCCAGGA 
AGACTCCAAG CAGTGTGGCC ACCCCCAAAG CCCAAAGATG AAGAAGAAAA AGTGGGTCTG 
AAATACACAG AAGCAGAGTA TCAGGCTGCT ATCCTGCATT TGAAGAGGGA ACACAAAGAT 
GAGCTAGAGA CACTGAAGTC CCAATCTGAA GTGGCGATCT TCAATGTGCG AGGGGAACAA 
GCAGTTCAGA CAGCAAAATT AGAGGAGGAA ATACAGAAAC TGCAAGCGGA CCTACAGAAT 
AAACTCAGTC ACCAGAATGG GGTGAAAAGG GATGTTTGTG TTTCCACCGA AGATGAGAAT 
TCTCCAAAAT CTTTTCGAAA TGTATGCATC CAAACTGACC GTGAGACTTT TTTGAAACCC 
AATGAGGAGG ACAAATCTCA AAAAAATGAC CCTGTTTTAC CAAAGAAATT AAATATTGCA 
TCAATCAGTC AAAACCTCGC TAGTCCCATA AGTCCCAGCG ATAATTCACA GCACAATTTC 
CCTTTAGCTT CCTTGTCAGT TACTACACCA CCTCCCCACC CCCTCTCTAC ATCTTCACAA 
ACAGCCTCTT CTCCAACACC CTTAGATTGC ACTTCTATTC CTCCACCTCC ATTACTTCCA 
GGTTTCCCTT CTGTTCCACC ACCTCCACCT CTTCCTGGTT CTAGCTCTGT TCCACCTCCT 
CCACCTCTTC CAGGTATCAG TTCTGCCCCA CCACCTCCCC CTCTTCCAGG TTTTAGCTCT 
GTTCCACCTC CTCCTCCTCC TCTTCCAGAT TTAAGTTCTG TTCCACCCCC TCCCCCTTTT 
CCAGGTGGGG GTCCTCCTCC ACCACCTCCT CCTTTTCCTG GGTATGGAAG CTCAGCAGTT 
CCTCCACCAC TACCTCTACC ACTCCCCGGG CTTTTTTTTG GGCTGTCAAA ACCTCGAAAA 
GCCCCTGTAG AGCCTGGCTG CCCAATGAAG CCGCTGTATT GGACAAGGAT TCAACTAAAA 
AATGGCAGCT CCAGCAATAC GTTATGGGAG AACCTAAAGG AGCCCAACAT TGCAGACACT 
AAAGAATTTG AGGACCTATT TGCAAAAGCC ACTGTTCAAC AGAAGAAGAA ACCACTGTCA 
GACTCTTATG AGAAGAGAGC AAAAGCTAAG CAGGTTATAA AGCTACTGGA TGGGAAGAGG 
TCTCAGGCTG TGGGCATTTT AATATCCAGT TTACATCTGG ACATGAAGGA TATTCAACAA 
GCAATTCTTA ATGTAGATAA TTCAGTTGTG GACATAGAAA CTCTTGAAGC CTTGTATGAA 
AACAGGGCTC AGAAGGAGGA GATGGAAATC ATCAAAAAAC ACTATCAGAC ATCTAAAGCT 
GAGGATGTAA AACTGCTGGA CAAACCTGAA CAGTTTCTTT ACGAGCTGTC TCAGATTCCC 
AACTTTGTTG AGCGATCTCA GTGTATTATA TTCCAGTCTG TCTTTTTAGA GGGAATAAGC 
TCAGTGAGGC GGAAGGTGGA TATCATCAGT CGGGCCTGCG ATTGCCTCTT GGAGAGGGTT 
TCTGTGAGAG ATATAATAGG TCTGATTCTT GCATTCGGAA ACTACATGAA TGGAGGCAAC 
CGTACTCGGG GTCAGGCAGA CGGATTTGGG CTGGAAATCC TTCCCAAACT AAAGGATGTT 
AAAAGCAGGG ATAATAAAGC CAGCCTGGTG GATTATGTTG TACGTTATTA TCTACGATAT 
TTTGACCAGG ATGCTGGAAC AGACAAAAGT GTTTTTCCAC TGCCAGAATC CCAGGACCTT 
TTACTGGCTT CGCAGGTGAA ATTTGAAGAC CTGGACAAAG ACTTACGGAA ACTTAAAAAG 
GACATTGAAG TGTGTGAGAA ACAGTTGAAA GCAGTGGTAA AAGACTCGCC TAAAGAGAAT 
ATCCAGCCAT TTAAGGACAA GATGTCATCT TTTTTAAACA AAGCCAAAGA AGAATATAAG 
ACAGAAGAAA GTAAACTGAC CAGATCACAA AACAGGTTTG AAGAAACAAT TGGATATTTT 
GGATGTAAGC CAAAGGGCAA TGATAAAGAG ATCACCCCCA ACTCCTTCTT TGTCTTGTGG 
TACGAATTCT GCGGTGACTT CAAGAGTGTG TGGAAAAGAG AGAGCAAAGT CCTCTCCACG 
GAAAGGCGCC TTTGTTTTCT TTTCGGATCA AATGGACATC AAGGGAAGGT GATTCCCCCC 
CAGGGCTCCA GAGGGAACCC GCTAGCGGCC GTCTGTCCTC ACGAAAAGAT AAACACGCCA 
GATCTGATAA GTTACACTTT TCTTTACCAC TCCCAATTAC TTGCTAGTAA AATGAAAGGA 
GACCTCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fmn1 ( XM_031890238.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746098.1 Xenopus tropicalis formin 1 (fmn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa63522
Clone ID Related Accession (Same CDS sequence)	XM_031890238.1
Accession Version	XM_031890238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	formin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCA CACACTCCAT CCTCCAATTG CACAAACCCA TTATGGAGCT CTGCTACGTC 
AGCTTCTTCC TTCCCAGAGG TAATGTCAGG GGATTCACAT ACAAACGCTG TGTAACTCGA 
GACAAAGCCA GAACATGTTT TGATAACTGT TATCGAATCA GCGAGGAGAG AGAGACTGCA 
GCACATAAAG ATCCACCTTA TGAAAGATTT ACAGAGGACC TGAAGGAAGC CACAACCCAA 
AACATACTGA CAGAGCTCTA CAAACTCACA GCTGCTAAAG ACAGACTTTT AGTTCAGCTC 
CTAGAAGCCT CCCATAAGCT AGGATCTATA ATGGGAAACC AGGATGGCAA ATTTCAGGAT 
CTTCCGGACG GATATAAATT TCCAGAAGAC GAGGCCCATG GCCATGTGGA TTTCCATGAG 
TCATTTTCAG CTTCAGTGGA GAAGAAGGTG CCTTCCACAA AGGTAAAGAA GAATAGAAGG 
CTTAGTCGGA GGAAAGAAAG TATAGAAGAT TTTGTGAACA AGAAGATAAA GTGGAAAAGT 
AGCACAGAAG CTTCTGCAAC ACAGGTTAGA GACAAAACGT CCTCAGCAAA GGTTTCCCTG 
ACGGGCTCTA CGGATTCATT GTCACGCCCA AAACTGCAGT CTGTGGAGAA CAAGGATAGT 
TACTTACCAG GGCCCATGGT GAAAAATGCA CAGGGTAAGC ACAGTGAAAG CTCAGGGATG 
CTGGGTTCTG AGTCTGCAAT GGGAAGCTTA TTGTCTGTGT ATGATAATGA TGTTTTTGCT 
GATTTTTCTC TGCTGCCTCA CAGTGACAGT CTTTTAAAAG AACTTCAGAG TGCAATTGAC 
ATTATGCCAG AGCAACCAAG CACCCAAGTA CTGGAAGGTT ATATATTTGA AAAGCATTGC 
ACTGAGCATG AGAGCAATGA GCAGGCATCC AGTGGGAGAA GTATAAAGAC AGTTACTGCT 
AAATTGCAGG ACATTAGCAC TTGTGTACAG AAAGTGGTTC ATACTTATAA CTATCAAGAA 
GACGGAGCTG AAGAAAGGAG GTGTGAAAAT ATTACTGAGC TCATTGATGA ACAGAAGGAG 
TTTGTATCAC AGATTGATGT ACCAATGATG TATGAACCAT TACAGTGTCA TATAGAAAAG 
CAGCTATATG GGGGTGTTTA TAATGACAGA CACGGTAGAC TAGAGACATG CAACAATAAT 
ATAGAGCCGC TGCCTTCCTG TGAATATGTC AACAAAAACC TTCTCAGGGT TGTCAAAAGT 
GAAAGCATGG ACGAAACAGA GTATTGGCTG GGACAGATGG ATAGGAGAGG AGAGAAGTGC 
AGTTATGGCC TTAAGCTGCC AGAGAAACTA AGTAAGTCAC AAGAATCATT AAACCATGTA 
ACTGTGCCAC ATAGTGCTCA TATTAAGTTA CAAGACAAAC CTACTGCAGT GAAAACACCA 
ACCTTTGATA CGGCCAGCAT CATGCATATA CCATGTACTA TGTGCAAAGG GATTTCCCCT 
GTTTCTTCTC CAGTATCTAC GCACCTGTCA AGCCCACAGC CCCATCATAG GATTCTCCCC 
TTGCAAACTC AACATGTAGA GGAAGATCTA AAGTTGTGTG GTGTTGAACA TTGGTCTGCC 
CTACCTGATA TTTTCAGAGA ACCATCATCA CTCCAAGAGC TGGGGTATGA TTTTAAAGGA 
TCTACCACGT TCAACATGGA CCCTGATAAC TATCTTTTTG ATGATTTCTT GCAAAATGTG 
AAAGGAAAAA AGACCACATT ACAGCCTAAT CTGCCTCCTC CAGGAGGTGG TGAAGCCTTG 
AAAGATGTTT CCAGAGGTCC AGATACTTCT TCTGGTCTCC AGGCCGGTGT CAGTGAGGAG 
CTGTCAAGTG TTTGTGATAA TAAAACAACT GTCGGGAGGC CCGACCACAG TATTTTGCAT 
CTCAGTCTGG AAATACATAC CGACCTCAGT CCTTCTGATG GAGAAGATCG AACCCCAGGA 
AGACTCCAAG CAGTGTGGCC ACCCCCAAAG CCCAAAGATG AAGAAGAAAA AGTGGGTCTG 
AAATACACAG AAGCAGAGTA TCAGGCTGCT ATCCTGCATT TGAAGAGGGA ACACAAAGAT 
GAGCTAGAGA CACTGAAGTC CCAATCTGAA GTGGCGATCT TCAATGTGCG AGGGGAACAA 
GCAGTTCAGA CAGCAAAATT AGAGGAGGAA ATACAGAAAC TGCAAGCGGA CCTACAGAAT 
AAACTCAGTC ACCAGAATGG GGTGAAAAGG GATGTTTGTG TTTCCACCGA AGATGAGAAT 
TCTCCAAAAT CTTTTCGAAA TGTATGCATC CAAACTGACC GTGAGACTTT TTTGAAACCC 
AATGAGGAGG ACAAATCTCA AAAAAATGAC CCTGTTTTAC CAAAGAAATT AAATATTGCA 
TCAATCAGTC AAAACCTCGC TAGTCCCATA AGTCCCAGCG ATAATTCACA GCACAATTTC 
CCTTTAGCTT CCTTGTCAGT TACTACACCA CCTCCCCACC CCCTCTCTAC ATCTTCACAA 
ACAGCCTCTT CTCCAACACC CTTAGATTGC ACTTCTATTC CTCCACCTCC ATTACTTCCA 
GGTTTCCCTT CTGTTCCACC ACCTCCACCT CTTCCTGGTT CTAGCTCTGT TCCACCTCCT 
CCACCTCTTC CAGGTATCAG TTCTGCCCCA CCACCTCCCC CTCTTCCAGG TTTTAGCTCT 
GTTCCACCTC CTCCTCCTCC TCTTCCAGAT TTAAGTTCTG TTCCACCCCC TCCCCCTTTT 
CCAGGTGGGG GTCCTCCTCC ACCACCTCCT CCTTTTCCTG GGTATGGAAG CTCAGCAGTT 
CCTCCACCAC TACCTCTACC ACTCCCCGGG CTTTTTTTTG GGCTGTCAAA ACCTCGAAAA 
GCCCCTGTAG AGCCTGGCTG CCCAATGAAG CCGCTGTATT GGACAAGGAT TCAACTAAAA 
AATGGCAGCT CCAGCAATAC GTTATGGGAG AACCTAAAGG AGCCCAACAT TGCAGACACT 
AAAGAATTTG AGGACCTATT TGCAAAAGCC ACTGTTCAAC AGAAGAAGAA ACCACTGTCA 
GACTCTTATG AGAAGAGAGC AAAAGCTAAG CAGGTTATAA AGCTACTGGA TGGGAAGAGG 
TCTCAGGCTG TGGGCATTTT AATATCCAGT TTACATCTGG ACATGAAGGA TATTCAACAA 
GCAATTCTTA ATGTAGATAA TTCAGTTGTG GACATAGAAA CTCTTGAAGC CTTGTATGAA 
AACAGGGCTC AGAAGGAGGA GATGGAAATC ATCAAAAAAC ACTATCAGAC ATCTAAAGCT 
GAGGATGTAA AACTGCTGGA CAAACCTGAA CAGTTTCTTT ACGAGCTGTC TCAGATTCCC 
AACTTTGTTG AGCGATCTCA GTGTATTATA TTCCAGTCTG TCTTTTTAGA GGGAATAAGC 
TCAGTGAGGC GGAAGGTGGA TATCATCAGT CGGGCCTGCG ATTGCCTCTT GGAGAGGGTT 
TCTGTGAGAG ATATAATAGG TCTGATTCTT GCATTCGGAA ACTACATGAA TGGAGGCAAC 
CGTACTCGGG GTCAGGCAGA CGGATTTGGG CTGGAAATCC TTCCCAAACT AAAGGATGTT 
AAAAGCAGGG ATAATAAAGC CAGCCTGGTG GATTATGTTG TACGTTATTA TCTACGATAT 
TTTGACCAGG ATGCTGGAAC AGACAAAAGT GTTTTTCCAC TGCCAGAATC CCAGGACCTT 
TTACTGGCTT CGCAGGTGAA ATTTGAAGAC CTGGACAAAG ACTTACGGAA ACTTAAAAAG 
GACATTGAAG TGTGTGAGAA ACAGTTGAAA GCAGTGGTAA AAGACTCGCC TAAAGAGAAT 
ATCCAGCCAT TTAAGGACAA GATGTCATCT TTTTTAAACA AAGCCAAAGA AGAATATAAG 
ACAGAAGAAA GTAAACTGAC CAGATCACAA AACAGGTTTG AAGAAACAAT TGGATATTTT 
GGATGTAAGC CAAAGGGCAA TGATAAAGAG ATCACCCCCA ACTCCTTCTT TGTCTTGTGG 
TACGAATTCT GCGGTGACTT CAAGAGTGTG TGGAAAAGAG AGAGCAAAGT CCTCTCCACG 
GAAAGGGAAG GTGATTCCCC CCCAGGGCTC CAGAGGGAAC CCGCTAGCGG CCGTCTGTCC 
TCACGAAAAG ATAAACACGC CAGATCTGAT AAGTTACACT TTTCTTTACC ACTCCCAATT 
ACTTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746098.1
CDS	326..4534
Translation	MEGTHSILQLHKPIMELCYVSFFLPRGNVRGFTYKRCVTRDKARTCFDNCYRISEERETAAHKDPPYERFTEDLKEATTQNILTELYKLTAAKDRLLVQLLEASHKLGSIMGNQDGKFQDLPDGYKFPEDEAHGHVDFHESFSASVEKKVPSTKVKKNRRLSRRKESIEDFVNKKIKWKSSTEASATQVRDKTSSAKVSLTGSTDSLSRPKLQSVENKDSYLPGPMVKNAQGKHSESSGMLGSESAMGSLLSVYDNDVFADFSLLPHSDSLLKELQSAIDIMPEQPSTQVLEGYIFEKHCTEHESNEQASSGRSIKTVTAKLQDISTCVQKVVHTYNYQEDGAEERRCENITELIDEQKEFVSQIDVPMMYEPLQCHIEKQLYGGVYNDRHGRLETCNNNIEPLPSCEYVNKNLLRVVKSESMDETEYWLGQMDRRGEKCSYGLKLPEKLSKSQESLNHVTVPHSAHIKLQDKPTAVKTPTFDTASIMHIPCTMCKGISPVSSPVSTHLSSPQPHHRILPLQTQHVEEDLKLCGVEHWSALPDIFREPSSLQELGYDFKGSTTFNMDPDNYLFDDFLQNVKGKKTTLQPNLPPPGGGEALKDVSRGPDTSSGLQAGVSEELSSVCDNKTTVGRPDHSILHLSLEIHTDLSPSDGEDRTPGRLQAVWPPPKPKDEEEKVGLKYTEAEYQAAILHLKREHKDELETLKSQSEVAIFNVRGEQAVQTAKLEEEIQKLQADLQNKLSHQNGVKRDVCVSTEDENSPKSFRNVCIQTDRETFLKPNEEDKSQKNDPVLPKKLNIASISQNLASPISPSDNSQHNFPLASLSVTTPPPHPLSTSSQTASSPTPLDCTSIPPPPLLPGFPSVPPPPPLPGSSSVPPPPPLPGISSAPPPPPLPGFSSVPPPPPPLPDLSSVPPPPPFPGGGPPPPPPPFPGYGSSAVPPPLPLPLPGLFFGLSKPRKAPVEPGCPMKPLYWTRIQLKNGSSSNTLWENLKEPNIADTKEFEDLFAKATVQQKKKPLSDSYEKRAKAKQVIKLLDGKRSQAVGILISSLHLDMKDIQQAILNVDNSVVDIETLEALYENRAQKEEMEIIKKHYQTSKAEDVKLLDKPEQFLYELSQIPNFVERSQCIIFQSVFLEGISSVRRKVDIISRACDCLLERVSVRDIIGLILAFGNYMNGGNRTRGQADGFGLEILPKLKDVKSRDNKASLVDYVVRYYLRYFDQDAGTDKSVFPLPESQDLLLASQVKFEDLDKDLRKLKKDIEVCEKQLKAVVKDSPKENIQPFKDKMSSFLNKAKEEYKTEESKLTRSQNRFEETIGYFGCKPKGNDKEITPNSFFVLWYEFCGDFKSVWKRESKVLSTEREGDSPPGLQREPASGRLSSRKDKHARSDKLHFSLPLPITC

Target ORF information:

RefSeq Version	XM_031890238.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis formin 1 (fmn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890238.1

ORF Insert Sequence:

ATGGAAGGCA CACACTCCAT CCTCCAATTG CACAAACCCA TTATGGAGCT CTGCTACGTC 
AGCTTCTTCC TTCCCAGAGG TAATGTCAGG GGATTCACAT ACAAACGCTG TGTAACTCGA 
GACAAAGCCA GAACATGTTT TGATAACTGT TATCGAATCA GCGAGGAGAG AGAGACTGCA 
GCACATAAAG ATCCACCTTA TGAAAGATTT ACAGAGGACC TGAAGGAAGC CACAACCCAA 
AACATACTGA CAGAGCTCTA CAAACTCACA GCTGCTAAAG ACAGACTTTT AGTTCAGCTC 
CTAGAAGCCT CCCATAAGCT AGGATCTATA ATGGGAAACC AGGATGGCAA ATTTCAGGAT 
CTTCCGGACG GATATAAATT TCCAGAAGAC GAGGCCCATG GCCATGTGGA TTTCCATGAG 
TCATTTTCAG CTTCAGTGGA GAAGAAGGTG CCTTCCACAA AGGTAAAGAA GAATAGAAGG 
CTTAGTCGGA GGAAAGAAAG TATAGAAGAT TTTGTGAACA AGAAGATAAA GTGGAAAAGT 
AGCACAGAAG CTTCTGCAAC ACAGGTTAGA GACAAAACGT CCTCAGCAAA GGTTTCCCTG 
ACGGGCTCTA CGGATTCATT GTCACGCCCA AAACTGCAGT CTGTGGAGAA CAAGGATAGT 
TACTTACCAG GGCCCATGGT GAAAAATGCA CAGGGTAAGC ACAGTGAAAG CTCAGGGATG 
CTGGGTTCTG AGTCTGCAAT GGGAAGCTTA TTGTCTGTGT ATGATAATGA TGTTTTTGCT 
GATTTTTCTC TGCTGCCTCA CAGTGACAGT CTTTTAAAAG AACTTCAGAG TGCAATTGAC 
ATTATGCCAG AGCAACCAAG CACCCAAGTA CTGGAAGGTT ATATATTTGA AAAGCATTGC 
ACTGAGCATG AGAGCAATGA GCAGGCATCC AGTGGGAGAA GTATAAAGAC AGTTACTGCT 
AAATTGCAGG ACATTAGCAC TTGTGTACAG AAAGTGGTTC ATACTTATAA CTATCAAGAA 
GACGGAGCTG AAGAAAGGAG GTGTGAAAAT ATTACTGAGC TCATTGATGA ACAGAAGGAG 
TTTGTATCAC AGATTGATGT ACCAATGATG TATGAACCAT TACAGTGTCA TATAGAAAAG 
CAGCTATATG GGGGTGTTTA TAATGACAGA CACGGTAGAC TAGAGACATG CAACAATAAT 
ATAGAGCCGC TGCCTTCCTG TGAATATGTC AACAAAAACC TTCTCAGGGT TGTCAAAAGT 
GAAAGCATGG ACGAAACAGA GTATTGGCTG GGACAGATGG ATAGGAGAGG AGAGAAGTGC 
AGTTATGGCC TTAAGCTGCC AGAGAAACTA AGTAAGTCAC AAGAATCATT AAACCATGTA 
ACTGTGCCAC ATAGTGCTCA TATTAAGTTA CAAGACAAAC CTACTGCAGT GAAAACACCA 
ACCTTTGATA CGGCCAGCAT CATGCATATA CCATGTACTA TGTGCAAAGG GATTTCCCCT 
GTTTCTTCTC CAGTATCTAC GCACCTGTCA AGCCCACAGC CCCATCATAG GATTCTCCCC 
TTGCAAACTC AACATGTAGA GGAAGATCTA AAGTTGTGTG GTGTTGAACA TTGGTCTGCC 
CTACCTGATA TTTTCAGAGA ACCATCATCA CTCCAAGAGC TGGGGTATGA TTTTAAAGGA 
TCTACCACGT TCAACATGGA CCCTGATAAC TATCTTTTTG ATGATTTCTT GCAAAATGTG 
AAAGGAAAAA AGACCACATT ACAGCCTAAT CTGCCTCCTC CAGGAGGTGG TGAAGCCTTG 
AAAGATGTTT CCAGAGGTCC AGATACTTCT TCTGGTCTCC AGGCCGGTGT CAGTGAGGAG 
CTGTCAAGTG TTTGTGATAA TAAAACAACT GTCGGGAGGC CCGACCACAG TATTTTGCAT 
CTCAGTCTGG AAATACATAC CGACCTCAGT CCTTCTGATG GAGAAGATCG AACCCCAGGA 
AGACTCCAAG CAGTGTGGCC ACCCCCAAAG CCCAAAGATG AAGAAGAAAA AGTGGGTCTG 
AAATACACAG AAGCAGAGTA TCAGGCTGCT ATCCTGCATT TGAAGAGGGA ACACAAAGAT 
GAGCTAGAGA CACTGAAGTC CCAATCTGAA GTGGCGATCT TCAATGTGCG AGGGGAACAA 
GCAGTTCAGA CAGCAAAATT AGAGGAGGAA ATACAGAAAC TGCAAGCGGA CCTACAGAAT 
AAACTCAGTC ACCAGAATGG GGTGAAAAGG GATGTTTGTG TTTCCACCGA AGATGAGAAT 
TCTCCAAAAT CTTTTCGAAA TGTATGCATC CAAACTGACC GTGAGACTTT TTTGAAACCC 
AATGAGGAGG ACAAATCTCA AAAAAATGAC CCTGTTTTAC CAAAGAAATT AAATATTGCA 
TCAATCAGTC AAAACCTCGC TAGTCCCATA AGTCCCAGCG ATAATTCACA GCACAATTTC 
CCTTTAGCTT CCTTGTCAGT TACTACACCA CCTCCCCACC CCCTCTCTAC ATCTTCACAA 
ACAGCCTCTT CTCCAACACC CTTAGATTGC ACTTCTATTC CTCCACCTCC ATTACTTCCA 
GGTTTCCCTT CTGTTCCACC ACCTCCACCT CTTCCTGGTT CTAGCTCTGT TCCACCTCCT 
CCACCTCTTC CAGGTATCAG TTCTGCCCCA CCACCTCCCC CTCTTCCAGG TTTTAGCTCT 
GTTCCACCTC CTCCTCCTCC TCTTCCAGAT TTAAGTTCTG TTCCACCCCC TCCCCCTTTT 
CCAGGTGGGG GTCCTCCTCC ACCACCTCCT CCTTTTCCTG GGTATGGAAG CTCAGCAGTT 
CCTCCACCAC TACCTCTACC ACTCCCCGGG CTTTTTTTTG GGCTGTCAAA ACCTCGAAAA 
GCCCCTGTAG AGCCTGGCTG CCCAATGAAG CCGCTGTATT GGACAAGGAT TCAACTAAAA 
AATGGCAGCT CCAGCAATAC GTTATGGGAG AACCTAAAGG AGCCCAACAT TGCAGACACT 
AAAGAATTTG AGGACCTATT TGCAAAAGCC ACTGTTCAAC AGAAGAAGAA ACCACTGTCA 
GACTCTTATG AGAAGAGAGC AAAAGCTAAG CAGGTTATAA AGCTACTGGA TGGGAAGAGG 
TCTCAGGCTG TGGGCATTTT AATATCCAGT TTACATCTGG ACATGAAGGA TATTCAACAA 
GCAATTCTTA ATGTAGATAA TTCAGTTGTG GACATAGAAA CTCTTGAAGC CTTGTATGAA 
AACAGGGCTC AGAAGGAGGA GATGGAAATC ATCAAAAAAC ACTATCAGAC ATCTAAAGCT 
GAGGATGTAA AACTGCTGGA CAAACCTGAA CAGTTTCTTT ACGAGCTGTC TCAGATTCCC 
AACTTTGTTG AGCGATCTCA GTGTATTATA TTCCAGTCTG TCTTTTTAGA GGGAATAAGC 
TCAGTGAGGC GGAAGGTGGA TATCATCAGT CGGGCCTGCG ATTGCCTCTT GGAGAGGGTT 
TCTGTGAGAG ATATAATAGG TCTGATTCTT GCATTCGGAA ACTACATGAA TGGAGGCAAC 
CGTACTCGGG GTCAGGCAGA CGGATTTGGG CTGGAAATCC TTCCCAAACT AAAGGATGTT 
AAAAGCAGGG ATAATAAAGC CAGCCTGGTG GATTATGTTG TACGTTATTA TCTACGATAT 
TTTGACCAGG ATGCTGGAAC AGACAAAAGT GTTTTTCCAC TGCCAGAATC CCAGGACCTT 
TTACTGGCTT CGCAGGTGAA ATTTGAAGAC CTGGACAAAG ACTTACGGAA ACTTAAAAAG 
GACATTGAAG TGTGTGAGAA ACAGTTGAAA GCAGTGGTAA AAGACTCGCC TAAAGAGAAT 
ATCCAGCCAT TTAAGGACAA GATGTCATCT TTTTTAAACA AAGCCAAAGA AGAATATAAG 
ACAGAAGAAA GTAAACTGAC CAGATCACAA AACAGGTTTG AAGAAACAAT TGGATATTTT 
GGATGTAAGC CAAAGGGCAA TGATAAAGAG ATCACCCCCA ACTCCTTCTT TGTCTTGTGG 
TACGAATTCT GCGGTGACTT CAAGAGTGTG TGGAAAAGAG AGAGCAAAGT CCTCTCCACG 
GAAAGGGAAG GTGATTCCCC CCCAGGGCTC CAGAGGGAAC CCGCTAGCGG CCGTCTGTCC 
TCACGAAAAG ATAAACACGC CAGATCTGAT AAGTTACACT TTTCTTTACC ACTCCCAATT 
ACTTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fmn1 ( XM_012968078.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823532.2 Xenopus tropicalis formin 1 (fmn1), transcript variant X3, mRNA.

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CloneID	OXa63523
Clone ID Related Accession (Same CDS sequence)	XM_012968078.3
Accession Version	XM_012968078.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	formin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968078.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGA AAGAGACTGC TACAAATATG CCAGAGGCGG CAGCTTCTGC TTCAGTAGGG 
AGACCTGATT CATCAGGTCT GTCTATACTG AGTAAATTTT CCATAAAGTC ATTTTTTGGA 
ATCACAACAA GGTTAGACAG CCAGAAAAGC AATGAAGGGA ATGCTGTTTT AAAATCTTTT 
AAATTACTTG GAAATAAAGC AGAGTTACAG ACAGACCCTG CGAGCAGTGC ACAGAATTCA 
AGCTGTGAGA GGTCAGTACC ACATTCTGAC ATAACAGGGT GGGAGGCAAA GGACCCTGAA 
GAGCCAAAGG CAACCACTGA TAAACATGTA CAAATTGGAC AGGCGGAAGG CTCAGATCTG 
GATTTGCAAC AGCACAGTTT AAAGGATCTA AGTTGTGAGA ATGGGGGGGA TTCACAAGTC 
ACAGTAGGAC AGCCTGCAGT AGGTGACATT GAAAAGGACA AAGAAAACGC TGAGAAGGAT 
AACCTGGGAG GTGCAGATGT ACTGTTTTTA GATGAGCAAA AGCATGAAAA AAAGGGAGTG 
TGCACAGAAA GTCTGACCGT TTTAGGCACT TGTAAGGATG AAAAAGCTGA TACACATTCC 
AGTGCAGAGC ACGCAGCCTG TGATGTTCCC AGCACAGGAG TTAATGACAA GGATGATTTG 
CTTGTACGTG GTATTCTTGT ACAGACAACA AGTGATACAG ACTCTGACGG TGAGGATACT 
GATCAAGTCA CAGACCAAAA GAGTGCTCCC ATGTCCAAGA ATTTCTCGGC ATCTCATGTG 
TCAGTAAACA ATAGTCCCAA ACAGGAGCCA GAAGCAACAG GTCCAGGAAA GACAGGGTAC 
ATGGAGGGAG AAGACAAAGA ACAAGTGAAT ATAAACAGCT CAGGCTCTTT GGGGGCCCAT 
ATGGATGCAC AACAAGGGAT TACACAGGAG GCAGAAATTA ACAGTCCTGA AGACAATTCC 
GTTCCAGAAT CTGTAACAGC TGCATCCCAT TCTGAGCCAC AGAATGAGAA GACCTATGTG 
CTTCCAGCAA TTTTTAGTGG TCTGCGTGTA CACAAGAAAG GAGCACTGGT GGATGATAGG 
GAGATGGTTA CTGTAAAACC AGGTGACAGT GATTTGGCAT TACTTAAATT GTCCCAGACA 
GCTCAAAAAT CCAAAGTCTC CATTGAAACA ATAAGAAAAC GAGAGCAGAA GAAGCCAACA 
GAAACCAGAC CAAGTTCAAA GTTCATGGAA CAACTTTCCC AGCTACTGAG CTTTGATAAC 
CCCAAGCAAG ATGAAACACA GGGGGGATCT ACAGAAGAAC AAAGTATTTC AAATGGCACT 
GAGCAAGACC TCCAGGCTGT TAATGAGGAC AAATTGGAAA CTAGCTCACA AGAGACTGCA 
ATGGATGCTT TCAAATCCTT TTTCATGATT GCTCCCAAAA AGACAATAAC ACAGGATTCC 
CATGACCTGG AGGCTGTCAA GCGCAAGCAA AAGAGTGAGA AGGATACACT GAAGGCTATC 
TTTGAGAAGC CTAGACCCAC AGACCTTGAC CTGGCATCTG ACGAAAAGAA AAATACCGAC 
CTCAGTCCTT CTGATGGAGA AGATCGAACC CCAGGAAGAC TCCAAGCAGT GTGGCCACCC 
CCAAAGCCCA AAGATGAAGA AGAAAAAGTG GGTCTGAAAT ACACAGAAGC AGAGTATCAG 
GCTGCTATCC TGCATTTGAA GAGGGAACAC AAAGATGAGC TAGAGACACT GAAGTCCCAA 
TCTGAAGTGG CGATCTTCAA TGTGCGAGGG GAACAAGCAG TTCAGACAGC AAAATTAGAG 
GAGGAAATAC AGAAACTGCA AGCGGACCTA CAGAATAAAC TCAGTCACCA GAATGGGGTG 
AAAAGGGATG TTTGTGTTTC CACCGAAGAT GAGAATTCTC CAAAATCTTT TCGAAATGTA 
TGCATCCAAA CTGACCGTGA GACTTTTTTG AAACCCAATG AGGAGGACAA ATCTCAAAAA 
AATGACCCTG TTTTACCAAA GAAATTAAAT ATTGCATCAA TCAGTCAAAA CCTCGCTAGT 
CCCATAAGTC CCAGCGATAA TTCACAGCAC AATTTCCCTT TAGCTTCCTT GTCAGTTACT 
ACACCACCTC CCCACCCCCT CTCTACATCT TCACAAACAG CCTCTTCTCC AACACCCTTA 
GATTGCACTT CTATTCCTCC ACCTCCATTA CTTCCAGGTT TCCCTTCTGT TCCACCACCT 
CCACCTCTTC CTGGTTCTAG CTCTGTTCCA CCTCCTCCAC CTCTTCCAGG TATCAGTTCT 
GCCCCACCAC CTCCCCCTCT TCCAGGTTTT AGCTCTGTTC CACCTCCTCC TCCTCCTCTT 
CCAGATTTAA GTTCTGTTCC ACCCCCTCCC CCTTTTCCAG GTGGGGGTCC TCCTCCACCA 
CCTCCTCCTT TTCCTGGGTA TGGAAGCTCA GCAGTTCCTC CACCACTACC TCTACCACTC 
CCCGGGCTTT TTTTTGGGCT GTCAAAACCT CGAAAAGCCC CTGTAGAGCC TGGCTGCCCA 
ATGAAGCCGC TGTATTGGAC AAGGATTCAA CTAAAAAATG GCAGCTCCAG CAATACGTTA 
TGGGAGAACC TAAAGGAGCC CAACATTGCA GACACTAAAG AATTTGAGGA CCTATTTGCA 
AAAGCCACTG TTCAACAGAA GAAGAAACCA CTGTCAGACT CTTATGAGAA GAGAGCAAAA 
GCTAAGCAGG TTATAAAGCT ACTGGATGGG AAGAGGTCTC AGGCTGTGGG CATTTTAATA 
TCCAGTTTAC ATCTGGACAT GAAGGATATT CAACAAGCAA TTCTTAATGT AGATAATTCA 
GTTGTGGACA TAGAAACTCT TGAAGCCTTG TATGAAAACA GGGCTCAGAA GGAGGAGATG 
GAAATCATCA AAAAACACTA TCAGACATCT AAAGCTGAGG ATGTAAAACT GCTGGACAAA 
CCTGAACAGT TTCTTTACGA GCTGTCTCAG ATTCCCAACT TTGTTGAGCG ATCTCAGTGT 
ATTATATTCC AGTCTGTCTT TTTAGAGGGA ATAAGCTCAG TGAGGCGGAA GGTGGATATC 
ATCAGTCGGG CCTGCGATTG CCTCTTGGAG AGGGTTTCTG TGAGAGATAT AATAGGTCTG 
ATTCTTGCAT TCGGAAACTA CATGAATGGA GGCAACCGTA CTCGGGGTCA GGCAGACGGA 
TTTGGGCTGG AAATCCTTCC CAAACTAAAG GATGTTAAAA GCAGGGATAA TAAAGCCAGC 
CTGGTGGATT ATGTTGTACG TTATTATCTA CGATATTTTG ACCAGGATGC TGGAACAGAC 
AAAAGTGTTT TTCCACTGCC AGAATCCCAG GACCTTTTAC TGGCTTCGCA GGTGAAATTT 
GAAGACCTGG ACAAAGACTT ACGGAAACTT AAAAAGGACA TTGAAGTGTG TGAGAAACAG 
TTGAAAGCAG TGGTAAAAGA CTCGCCTAAA GAGAATATCC AGCCATTTAA GGACAAGATG 
TCATCTTTTT TAAACAAAGC CAAAGAAGAA TATAAGACAG AAGAAAGTAA ACTGACCAGA 
TCACAAAACA GGTTTGAAGA AACAATTGGA TATTTTGGAT GTAAGCCAAA GGGCAATGAT 
AAAGAGATCA CCCCCAACTC CTTCTTTGTC TTGTGGTACG AATTCTGCGG TGACTTCAAG 
AGTGTGTGGA AAAGAGAGAG CAAAGTCCTC TCCACGGAAA GGCGCCTTTG TTTTCTTTTC 
GGATCAAATG GACATCAAGG GAAGGTGATT CCCCCCCAGG GCTCCAGAGG GAACCCGCTA 
GCGGCCGTCT GTCCTCACGA AAAGATAAAC ACGCCAGATC TGATAAGTTA CACTTTTCTT 
TACCACTCCC AATTACTTGC TAGTAAAATG AAAGGAGACC TCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823532.2
CDS	688..4575
Translation	MELKETATNMPEAAASASVGRPDSSGLSILSKFSIKSFFGITTRLDSQKSNEGNAVLKSFKLLGNKAELQTDPASSAQNSSCERSVPHSDITGWEAKDPEEPKATTDKHVQIGQAEGSDLDLQQHSLKDLSCENGGDSQVTVGQPAVGDIEKDKENAEKDNLGGADVLFLDEQKHEKKGVCTESLTVLGTCKDEKADTHSSAEHAACDVPSTGVNDKDDLLVRGILVQTTSDTDSDGEDTDQVTDQKSAPMSKNFSASHVSVNNSPKQEPEATGPGKTGYMEGEDKEQVNINSSGSLGAHMDAQQGITQEAEINSPEDNSVPESVTAASHSEPQNEKTYVLPAIFSGLRVHKKGALVDDREMVTVKPGDSDLALLKLSQTAQKSKVSIETIRKREQKKPTETRPSSKFMEQLSQLLSFDNPKQDETQGGSTEEQSISNGTEQDLQAVNEDKLETSSQETAMDAFKSFFMIAPKKTITQDSHDLEAVKRKQKSEKDTLKAIFEKPRPTDLDLASDEKKNTDLSPSDGEDRTPGRLQAVWPPPKPKDEEEKVGLKYTEAEYQAAILHLKREHKDELETLKSQSEVAIFNVRGEQAVQTAKLEEEIQKLQADLQNKLSHQNGVKRDVCVSTEDENSPKSFRNVCIQTDRETFLKPNEEDKSQKNDPVLPKKLNIASISQNLASPISPSDNSQHNFPLASLSVTTPPPHPLSTSSQTASSPTPLDCTSIPPPPLLPGFPSVPPPPPLPGSSSVPPPPPLPGISSAPPPPPLPGFSSVPPPPPPLPDLSSVPPPPPFPGGGPPPPPPPFPGYGSSAVPPPLPLPLPGLFFGLSKPRKAPVEPGCPMKPLYWTRIQLKNGSSSNTLWENLKEPNIADTKEFEDLFAKATVQQKKKPLSDSYEKRAKAKQVIKLLDGKRSQAVGILISSLHLDMKDIQQAILNVDNSVVDIETLEALYENRAQKEEMEIIKKHYQTSKAEDVKLLDKPEQFLYELSQIPNFVERSQCIIFQSVFLEGISSVRRKVDIISRACDCLLERVSVRDIIGLILAFGNYMNGGNRTRGQADGFGLEILPKLKDVKSRDNKASLVDYVVRYYLRYFDQDAGTDKSVFPLPESQDLLLASQVKFEDLDKDLRKLKKDIEVCEKQLKAVVKDSPKENIQPFKDKMSSFLNKAKEEYKTEESKLTRSQNRFEETIGYFGCKPKGNDKEITPNSFFVLWYEFCGDFKSVWKRESKVLSTERRLCFLFGSNGHQGKVIPPQGSRGNPLAAVCPHEKINTPDLISYTFLYHSQLLASKMKGDLS

Target ORF information:

RefSeq Version	XM_012968078.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis formin 1 (fmn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968078.3

ORF Insert Sequence:

ATGGAGCTGA AAGAGACTGC TACAAATATG CCAGAGGCGG CAGCTTCTGC TTCAGTAGGG 
AGACCTGATT CATCAGGTCT GTCTATACTG AGTAAATTTT CCATAAAGTC ATTTTTTGGA 
ATCACAACAA GGTTAGACAG CCAGAAAAGC AATGAAGGGA ATGCTGTTTT AAAATCTTTT 
AAATTACTTG GAAATAAAGC AGAGTTACAG ACAGACCCTG CGAGCAGTGC ACAGAATTCA 
AGCTGTGAGA GGTCAGTACC ACATTCTGAC ATAACAGGGT GGGAGGCAAA GGACCCTGAA 
GAGCCAAAGG CAACCACTGA TAAACATGTA CAAATTGGAC AGGCGGAAGG CTCAGATCTG 
GATTTGCAAC AGCACAGTTT AAAGGATCTA AGTTGTGAGA ATGGGGGGGA TTCACAAGTC 
ACAGTAGGAC AGCCTGCAGT AGGTGACATT GAAAAGGACA AAGAAAACGC TGAGAAGGAT 
AACCTGGGAG GTGCAGATGT ACTGTTTTTA GATGAGCAAA AGCATGAAAA AAAGGGAGTG 
TGCACAGAAA GTCTGACCGT TTTAGGCACT TGTAAGGATG AAAAAGCTGA TACACATTCC 
AGTGCAGAGC ACGCAGCCTG TGATGTTCCC AGCACAGGAG TTAATGACAA GGATGATTTG 
CTTGTACGTG GTATTCTTGT ACAGACAACA AGTGATACAG ACTCTGACGG TGAGGATACT 
GATCAAGTCA CAGACCAAAA GAGTGCTCCC ATGTCCAAGA ATTTCTCGGC ATCTCATGTG 
TCAGTAAACA ATAGTCCCAA ACAGGAGCCA GAAGCAACAG GTCCAGGAAA GACAGGGTAC 
ATGGAGGGAG AAGACAAAGA ACAAGTGAAT ATAAACAGCT CAGGCTCTTT GGGGGCCCAT 
ATGGATGCAC AACAAGGGAT TACACAGGAG GCAGAAATTA ACAGTCCTGA AGACAATTCC 
GTTCCAGAAT CTGTAACAGC TGCATCCCAT TCTGAGCCAC AGAATGAGAA GACCTATGTG 
CTTCCAGCAA TTTTTAGTGG TCTGCGTGTA CACAAGAAAG GAGCACTGGT GGATGATAGG 
GAGATGGTTA CTGTAAAACC AGGTGACAGT GATTTGGCAT TACTTAAATT GTCCCAGACA 
GCTCAAAAAT CCAAAGTCTC CATTGAAACA ATAAGAAAAC GAGAGCAGAA GAAGCCAACA 
GAAACCAGAC CAAGTTCAAA GTTCATGGAA CAACTTTCCC AGCTACTGAG CTTTGATAAC 
CCCAAGCAAG ATGAAACACA GGGGGGATCT ACAGAAGAAC AAAGTATTTC AAATGGCACT 
GAGCAAGACC TCCAGGCTGT TAATGAGGAC AAATTGGAAA CTAGCTCACA AGAGACTGCA 
ATGGATGCTT TCAAATCCTT TTTCATGATT GCTCCCAAAA AGACAATAAC ACAGGATTCC 
CATGACCTGG AGGCTGTCAA GCGCAAGCAA AAGAGTGAGA AGGATACACT GAAGGCTATC 
TTTGAGAAGC CTAGACCCAC AGACCTTGAC CTGGCATCTG ACGAAAAGAA AAATACCGAC 
CTCAGTCCTT CTGATGGAGA AGATCGAACC CCAGGAAGAC TCCAAGCAGT GTGGCCACCC 
CCAAAGCCCA AAGATGAAGA AGAAAAAGTG GGTCTGAAAT ACACAGAAGC AGAGTATCAG 
GCTGCTATCC TGCATTTGAA GAGGGAACAC AAAGATGAGC TAGAGACACT GAAGTCCCAA 
TCTGAAGTGG CGATCTTCAA TGTGCGAGGG GAACAAGCAG TTCAGACAGC AAAATTAGAG 
GAGGAAATAC AGAAACTGCA AGCGGACCTA CAGAATAAAC TCAGTCACCA GAATGGGGTG 
AAAAGGGATG TTTGTGTTTC CACCGAAGAT GAGAATTCTC CAAAATCTTT TCGAAATGTA 
TGCATCCAAA CTGACCGTGA GACTTTTTTG AAACCCAATG AGGAGGACAA ATCTCAAAAA 
AATGACCCTG TTTTACCAAA GAAATTAAAT ATTGCATCAA TCAGTCAAAA CCTCGCTAGT 
CCCATAAGTC CCAGCGATAA TTCACAGCAC AATTTCCCTT TAGCTTCCTT GTCAGTTACT 
ACACCACCTC CCCACCCCCT CTCTACATCT TCACAAACAG CCTCTTCTCC AACACCCTTA 
GATTGCACTT CTATTCCTCC ACCTCCATTA CTTCCAGGTT TCCCTTCTGT TCCACCACCT 
CCACCTCTTC CTGGTTCTAG CTCTGTTCCA CCTCCTCCAC CTCTTCCAGG TATCAGTTCT 
GCCCCACCAC CTCCCCCTCT TCCAGGTTTT AGCTCTGTTC CACCTCCTCC TCCTCCTCTT 
CCAGATTTAA GTTCTGTTCC ACCCCCTCCC CCTTTTCCAG GTGGGGGTCC TCCTCCACCA 
CCTCCTCCTT TTCCTGGGTA TGGAAGCTCA GCAGTTCCTC CACCACTACC TCTACCACTC 
CCCGGGCTTT TTTTTGGGCT GTCAAAACCT CGAAAAGCCC CTGTAGAGCC TGGCTGCCCA 
ATGAAGCCGC TGTATTGGAC AAGGATTCAA CTAAAAAATG GCAGCTCCAG CAATACGTTA 
TGGGAGAACC TAAAGGAGCC CAACATTGCA GACACTAAAG AATTTGAGGA CCTATTTGCA 
AAAGCCACTG TTCAACAGAA GAAGAAACCA CTGTCAGACT CTTATGAGAA GAGAGCAAAA 
GCTAAGCAGG TTATAAAGCT ACTGGATGGG AAGAGGTCTC AGGCTGTGGG CATTTTAATA 
TCCAGTTTAC ATCTGGACAT GAAGGATATT CAACAAGCAA TTCTTAATGT AGATAATTCA 
GTTGTGGACA TAGAAACTCT TGAAGCCTTG TATGAAAACA GGGCTCAGAA GGAGGAGATG 
GAAATCATCA AAAAACACTA TCAGACATCT AAAGCTGAGG ATGTAAAACT GCTGGACAAA 
CCTGAACAGT TTCTTTACGA GCTGTCTCAG ATTCCCAACT TTGTTGAGCG ATCTCAGTGT 
ATTATATTCC AGTCTGTCTT TTTAGAGGGA ATAAGCTCAG TGAGGCGGAA GGTGGATATC 
ATCAGTCGGG CCTGCGATTG CCTCTTGGAG AGGGTTTCTG TGAGAGATAT AATAGGTCTG 
ATTCTTGCAT TCGGAAACTA CATGAATGGA GGCAACCGTA CTCGGGGTCA GGCAGACGGA 
TTTGGGCTGG AAATCCTTCC CAAACTAAAG GATGTTAAAA GCAGGGATAA TAAAGCCAGC 
CTGGTGGATT ATGTTGTACG TTATTATCTA CGATATTTTG ACCAGGATGC TGGAACAGAC 
AAAAGTGTTT TTCCACTGCC AGAATCCCAG GACCTTTTAC TGGCTTCGCA GGTGAAATTT 
GAAGACCTGG ACAAAGACTT ACGGAAACTT AAAAAGGACA TTGAAGTGTG TGAGAAACAG 
TTGAAAGCAG TGGTAAAAGA CTCGCCTAAA GAGAATATCC AGCCATTTAA GGACAAGATG 
TCATCTTTTT TAAACAAAGC CAAAGAAGAA TATAAGACAG AAGAAAGTAA ACTGACCAGA 
TCACAAAACA GGTTTGAAGA AACAATTGGA TATTTTGGAT GTAAGCCAAA GGGCAATGAT 
AAAGAGATCA CCCCCAACTC CTTCTTTGTC TTGTGGTACG AATTCTGCGG TGACTTCAAG 
AGTGTGTGGA AAAGAGAGAG CAAAGTCCTC TCCACGGAAA GGCGCCTTTG TTTTCTTTTC 
GGATCAAATG GACATCAAGG GAAGGTGATT CCCCCCCAGG GCTCCAGAGG GAACCCGCTA 
GCGGCCGTCT GTCCTCACGA AAAGATAAAC ACGCCAGATC TGATAAGTTA CACTTTTCTT 
TACCACTCCC AATTACTTGC TAGTAAAATG AAAGGAGACC TCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fmn1 ( XM_012968079.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823533.2 Xenopus tropicalis formin 1 (fmn1), transcript variant X4, mRNA.

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CloneID	OXa63524
Clone ID Related Accession (Same CDS sequence)	XM_012968079.3
Accession Version	XM_012968079.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	formin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968079.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGA AAGAGACTGC TACAAATATG CCAGAGGCGG CAGCTTCTGC TTCAGTAGGG 
AGACCTGATT CATCAGGTCT GTCTATACTG AGTAAATTTT CCATAAAGTC ATTTTTTGGA 
ATCACAACAA GGTTAGACAG CCAGAAAAGC AATGAAGGGA ATGCTGTTTT AAAATCTTTT 
AAATTACTTG GAAATAAAGC AGAGTTACAG ACAGACCCTG CGAGCAGTGC ACAGAATTCA 
AGCTGTGAGA GGTCAGTACC ACATTCTGAC ATAACAGGGT GGGAGGCAAA GGACCCTGAA 
GAGCCAAAGG CAACCACTGA TAAACATGTA CAAATTGGAC AGGCGGAAGG CTCAGATCTG 
GATTTGCAAC AGCACAGTTT AAAGGATCTA AGTTGTGAGA ATGGGGGGGA TTCACAAGTC 
ACAGTAGGAC AGCCTGCAGT AGGTGACATT GAAAAGGACA AAGAAAACGC TGAGAAGGAT 
AACCTGGGAG GTGCAGATGT ACTGTTTTTA GATGAGCAAA AGCATGAAAA AAAGGGAGTG 
TGCACAGAAA GTCTGACCGT TTTAGGCACT TGTAAGGATG AAAAAGCTGA TACACATTCC 
AGTGCAGAGC ACGCAGCCTG TGATGTTCCC AGCACAGGAG TTAATGACAA GGATGATTTG 
CTTGTACGTG GTATTCTTGT ACAGACAACA AGTGATACAG ACTCTGACGG TGAGGATACT 
GATCAAGTCA CAGACCAAAA GAGTGCTCCC ATGTCCAAGA ATTTCTCGGC ATCTCATGTG 
TCAGTAAACA ATAGTCCCAA ACAGGAGCCA GAAGCAACAG GTCCAGGAAA GACAGGGTAC 
ATGGAGGGAG AAGACAAAGA ACAAGTGAAT ATAAACAGCT CAGGCTCTTT GGGGGCCCAT 
ATGGATGCAC AACAAGGGAT TACACAGGAG GCAGAAATTA ACAGTCCTGA AGACAATTCC 
GTTCCAGAAT CTGTAACAGC TGCATCCCAT TCTGAGCCAC AGAATGAGAA GACCTATGTG 
CTTCCAGCAA TTTTTAGTGG TCTGCGTGTA CACAAGAAAG GAGCACTGGT GGATGATAGG 
GAGATGGTTA CTGTAAAACC AGGTGACAGT GATTTGGCAT TACTTAAATT GTCCCAGACA 
GCTCAAAAAT CCAAAGTCTC CATTGAAACA ATAAGAAAAC GAGAGCAGAA GAAGCCAACA 
GAAACCAGAC CAAGTTCAAA GTTCATGGAA CAACTTTCCC AGCTACTGAG CTTTGATAAC 
CCCAAGCAAG ATGAAACACA GGGGGGATCT ACAGAAGAAC AAAGTATTTC AAATGGCACT 
GAGCAAGACC TCCAGGCTGT TAATGAGGAC AAATTGGAAA CTAGCTCACA AGAGACTGCA 
ATGGATGCTT TCAAATCCTT TTTCATGATT GCTCCCAAAA AGACAATAAC ACAGGATTCC 
CATGACCTGG AGGCTGTCAA GCGCAAGCAA AAGAGTGAGA AGGATACACT GAAGGCTATC 
TTTGAGAAGC CTAGACCCAC AGACCTTGAC CTGGCATCTG ACGAAAAGAA AAATACCGAC 
CTCAGTCCTT CTGATGGAGA AGATCGAACC CCAGGAAGAC TCCAAGCAGT GTGGCCACCC 
CCAAAGCCCA AAGATGAAGA AGAAAAAGTG GGTCTGAAAT ACACAGAAGC AGAGTATCAG 
GCTGCTATCC TGCATTTGAA GAGGGAACAC AAAGATGAGC TAGAGACACT GAAGTCCCAA 
TCTGAAGTGG CGATCTTCAA TGTGCGAGGG GAACAAGCAG TTCAGACAGC AAAATTAGAG 
GAGGAAATAC AGAAACTGCA AGCGGACCTA CAGAATAAAC TCAGTCACCA GAATGGGGTG 
AAAAGGGATG TTTGTGTTTC CACCGAAGAT GAGAATTCTC CAAAATCTTT TCGAAATGTA 
TGCATCCAAA CTGACCGTGA GACTTTTTTG AAACCCAATG AGGAGGACAA ATCTCAAAAA 
AATGACCCTG TTTTACCAAA GAAATTAAAT ATTGCATCAA TCAGTCAAAA CCTCGCTAGT 
CCCATAAGTC CCAGCGATAA TTCACAGCAC AATTTCCCTT TAGCTTCCTT GTCAGTTACT 
ACACCACCTC CCCACCCCCT CTCTACATCT TCACAAACAG CCTCTTCTCC AACACCCTTA 
GATTGCACTT CTATTCCTCC ACCTCCATTA CTTCCAGGTT TCCCTTCTGT TCCACCACCT 
CCACCTCTTC CTGGTTCTAG CTCTGTTCCA CCTCCTCCAC CTCTTCCAGG TATCAGTTCT 
GCCCCACCAC CTCCCCCTCT TCCAGGTTTT AGCTCTGTTC CACCTCCTCC TCCTCCTCTT 
CCAGATTTAA GTTCTGTTCC ACCCCCTCCC CCTTTTCCAG GTGGGGGTCC TCCTCCACCA 
CCTCCTCCTT TTCCTGGGTA TGGAAGCTCA GCAGTTCCTC CACCACTACC TCTACCACTC 
CCCGGGCTTT TTTTTGGGCT GTCAAAACCT CGAAAAGCCC CTGTAGAGCC TGGCTGCCCA 
ATGAAGCCGC TGTATTGGAC AAGGATTCAA CTAAAAAATG GCAGCTCCAG CAATACGTTA 
TGGGAGAACC TAAAGGAGCC CAACATTGCA GACACTAAAG AATTTGAGGA CCTATTTGCA 
AAAGCCACTG TTCAACAGAA GAAGAAACCA CTGTCAGACT CTTATGAGAA GAGAGCAAAA 
GCTAAGCAGG TTATAAAGCT ACTGGATGGG AAGAGGTCTC AGGCTGTGGG CATTTTAATA 
TCCAGTTTAC ATCTGGACAT GAAGGATATT CAACAAGCAA TTCTTAATGT AGATAATTCA 
GTTGTGGACA TAGAAACTCT TGAAGCCTTG TATGAAAACA GGGCTCAGAA GGAGGAGATG 
GAAATCATCA AAAAACACTA TCAGACATCT AAAGCTGAGG ATGTAAAACT GCTGGACAAA 
CCTGAACAGT TTCTTTACGA GCTGTCTCAG ATTCCCAACT TTGTTGAGCG ATCTCAGTGT 
ATTATATTCC AGTCTGTCTT TTTAGAGGGA ATAAGCTCAG TGAGGCGGAA GGTGGATATC 
ATCAGTCGGG CCTGCGATTG CCTCTTGGAG AGGGTTTCTG TGAGAGATAT AATAGGTCTG 
ATTCTTGCAT TCGGAAACTA CATGAATGGA GGCAACCGTA CTCGGGGTCA GGCAGACGGA 
TTTGGGCTGG AAATCCTTCC CAAACTAAAG GATGTTAAAA GCAGGGATAA TAAAGCCAGC 
CTGGTGGATT ATGTTGTACG TTATTATCTA CGATATTTTG ACCAGGATGC TGGAACAGAC 
AAAAGTGTTT TTCCACTGCC AGAATCCCAG GACCTTTTAC TGGCTTCGCA GGTGAAATTT 
GAAGACCTGG ACAAAGACTT ACGGAAACTT AAAAAGGACA TTGAAGTGTG TGAGAAACAG 
TTGAAAGCAG TGGTAAAAGA CTCGCCTAAA GAGAATATCC AGCCATTTAA GGACAAGATG 
TCATCTTTTT TAAACAAAGC CAAAGAAGAA TATAAGACAG AAGAAAGTAA ACTGACCAGA 
TCACAAAACA GGTTTGAAGA AACAATTGGA TATTTTGGAT GTAAGCCAAA GGGCAATGAT 
AAAGAGATCA CCCCCAACTC CTTCTTTGTC TTGTGGTACG AATTCTGCGG TGACTTCAAG 
AGTGTGTGGA AAAGAGAGAG CAAAGTCCTC TCCACGGAAA GGCTTCGCCA GGCTCAGGAG 
TCCGTAAATA AACTGACAGC AGAAAAGAAA GTTGAAACCA GGAAGATTAA CCCTGCAGCA 
AGTCTGAAAG AAAAGCTTCG TCAGAAAGAA GCAAGTGTCA GCACCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823533.2
CDS	681..4511
Translation	MELKETATNMPEAAASASVGRPDSSGLSILSKFSIKSFFGITTRLDSQKSNEGNAVLKSFKLLGNKAELQTDPASSAQNSSCERSVPHSDITGWEAKDPEEPKATTDKHVQIGQAEGSDLDLQQHSLKDLSCENGGDSQVTVGQPAVGDIEKDKENAEKDNLGGADVLFLDEQKHEKKGVCTESLTVLGTCKDEKADTHSSAEHAACDVPSTGVNDKDDLLVRGILVQTTSDTDSDGEDTDQVTDQKSAPMSKNFSASHVSVNNSPKQEPEATGPGKTGYMEGEDKEQVNINSSGSLGAHMDAQQGITQEAEINSPEDNSVPESVTAASHSEPQNEKTYVLPAIFSGLRVHKKGALVDDREMVTVKPGDSDLALLKLSQTAQKSKVSIETIRKREQKKPTETRPSSKFMEQLSQLLSFDNPKQDETQGGSTEEQSISNGTEQDLQAVNEDKLETSSQETAMDAFKSFFMIAPKKTITQDSHDLEAVKRKQKSEKDTLKAIFEKPRPTDLDLASDEKKNTDLSPSDGEDRTPGRLQAVWPPPKPKDEEEKVGLKYTEAEYQAAILHLKREHKDELETLKSQSEVAIFNVRGEQAVQTAKLEEEIQKLQADLQNKLSHQNGVKRDVCVSTEDENSPKSFRNVCIQTDRETFLKPNEEDKSQKNDPVLPKKLNIASISQNLASPISPSDNSQHNFPLASLSVTTPPPHPLSTSSQTASSPTPLDCTSIPPPPLLPGFPSVPPPPPLPGSSSVPPPPPLPGISSAPPPPPLPGFSSVPPPPPPLPDLSSVPPPPPFPGGGPPPPPPPFPGYGSSAVPPPLPLPLPGLFFGLSKPRKAPVEPGCPMKPLYWTRIQLKNGSSSNTLWENLKEPNIADTKEFEDLFAKATVQQKKKPLSDSYEKRAKAKQVIKLLDGKRSQAVGILISSLHLDMKDIQQAILNVDNSVVDIETLEALYENRAQKEEMEIIKKHYQTSKAEDVKLLDKPEQFLYELSQIPNFVERSQCIIFQSVFLEGISSVRRKVDIISRACDCLLERVSVRDIIGLILAFGNYMNGGNRTRGQADGFGLEILPKLKDVKSRDNKASLVDYVVRYYLRYFDQDAGTDKSVFPLPESQDLLLASQVKFEDLDKDLRKLKKDIEVCEKQLKAVVKDSPKENIQPFKDKMSSFLNKAKEEYKTEESKLTRSQNRFEETIGYFGCKPKGNDKEITPNSFFVLWYEFCGDFKSVWKRESKVLSTERLRQAQESVNKLTAEKKVETRKINPAASLKEKLRQKEASVSTN

Target ORF information:

RefSeq Version	XM_012968079.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis formin 1 (fmn1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968079.3

ORF Insert Sequence:

ATGGAGCTGA AAGAGACTGC TACAAATATG CCAGAGGCGG CAGCTTCTGC TTCAGTAGGG 
AGACCTGATT CATCAGGTCT GTCTATACTG AGTAAATTTT CCATAAAGTC ATTTTTTGGA 
ATCACAACAA GGTTAGACAG CCAGAAAAGC AATGAAGGGA ATGCTGTTTT AAAATCTTTT 
AAATTACTTG GAAATAAAGC AGAGTTACAG ACAGACCCTG CGAGCAGTGC ACAGAATTCA 
AGCTGTGAGA GGTCAGTACC ACATTCTGAC ATAACAGGGT GGGAGGCAAA GGACCCTGAA 
GAGCCAAAGG CAACCACTGA TAAACATGTA CAAATTGGAC AGGCGGAAGG CTCAGATCTG 
GATTTGCAAC AGCACAGTTT AAAGGATCTA AGTTGTGAGA ATGGGGGGGA TTCACAAGTC 
ACAGTAGGAC AGCCTGCAGT AGGTGACATT GAAAAGGACA AAGAAAACGC TGAGAAGGAT 
AACCTGGGAG GTGCAGATGT ACTGTTTTTA GATGAGCAAA AGCATGAAAA AAAGGGAGTG 
TGCACAGAAA GTCTGACCGT TTTAGGCACT TGTAAGGATG AAAAAGCTGA TACACATTCC 
AGTGCAGAGC ACGCAGCCTG TGATGTTCCC AGCACAGGAG TTAATGACAA GGATGATTTG 
CTTGTACGTG GTATTCTTGT ACAGACAACA AGTGATACAG ACTCTGACGG TGAGGATACT 
GATCAAGTCA CAGACCAAAA GAGTGCTCCC ATGTCCAAGA ATTTCTCGGC ATCTCATGTG 
TCAGTAAACA ATAGTCCCAA ACAGGAGCCA GAAGCAACAG GTCCAGGAAA GACAGGGTAC 
ATGGAGGGAG AAGACAAAGA ACAAGTGAAT ATAAACAGCT CAGGCTCTTT GGGGGCCCAT 
ATGGATGCAC AACAAGGGAT TACACAGGAG GCAGAAATTA ACAGTCCTGA AGACAATTCC 
GTTCCAGAAT CTGTAACAGC TGCATCCCAT TCTGAGCCAC AGAATGAGAA GACCTATGTG 
CTTCCAGCAA TTTTTAGTGG TCTGCGTGTA CACAAGAAAG GAGCACTGGT GGATGATAGG 
GAGATGGTTA CTGTAAAACC AGGTGACAGT GATTTGGCAT TACTTAAATT GTCCCAGACA 
GCTCAAAAAT CCAAAGTCTC CATTGAAACA ATAAGAAAAC GAGAGCAGAA GAAGCCAACA 
GAAACCAGAC CAAGTTCAAA GTTCATGGAA CAACTTTCCC AGCTACTGAG CTTTGATAAC 
CCCAAGCAAG ATGAAACACA GGGGGGATCT ACAGAAGAAC AAAGTATTTC AAATGGCACT 
GAGCAAGACC TCCAGGCTGT TAATGAGGAC AAATTGGAAA CTAGCTCACA AGAGACTGCA 
ATGGATGCTT TCAAATCCTT TTTCATGATT GCTCCCAAAA AGACAATAAC ACAGGATTCC 
CATGACCTGG AGGCTGTCAA GCGCAAGCAA AAGAGTGAGA AGGATACACT GAAGGCTATC 
TTTGAGAAGC CTAGACCCAC AGACCTTGAC CTGGCATCTG ACGAAAAGAA AAATACCGAC 
CTCAGTCCTT CTGATGGAGA AGATCGAACC CCAGGAAGAC TCCAAGCAGT GTGGCCACCC 
CCAAAGCCCA AAGATGAAGA AGAAAAAGTG GGTCTGAAAT ACACAGAAGC AGAGTATCAG 
GCTGCTATCC TGCATTTGAA GAGGGAACAC AAAGATGAGC TAGAGACACT GAAGTCCCAA 
TCTGAAGTGG CGATCTTCAA TGTGCGAGGG GAACAAGCAG TTCAGACAGC AAAATTAGAG 
GAGGAAATAC AGAAACTGCA AGCGGACCTA CAGAATAAAC TCAGTCACCA GAATGGGGTG 
AAAAGGGATG TTTGTGTTTC CACCGAAGAT GAGAATTCTC CAAAATCTTT TCGAAATGTA 
TGCATCCAAA CTGACCGTGA GACTTTTTTG AAACCCAATG AGGAGGACAA ATCTCAAAAA 
AATGACCCTG TTTTACCAAA GAAATTAAAT ATTGCATCAA TCAGTCAAAA CCTCGCTAGT 
CCCATAAGTC CCAGCGATAA TTCACAGCAC AATTTCCCTT TAGCTTCCTT GTCAGTTACT 
ACACCACCTC CCCACCCCCT CTCTACATCT TCACAAACAG CCTCTTCTCC AACACCCTTA 
GATTGCACTT CTATTCCTCC ACCTCCATTA CTTCCAGGTT TCCCTTCTGT TCCACCACCT 
CCACCTCTTC CTGGTTCTAG CTCTGTTCCA CCTCCTCCAC CTCTTCCAGG TATCAGTTCT 
GCCCCACCAC CTCCCCCTCT TCCAGGTTTT AGCTCTGTTC CACCTCCTCC TCCTCCTCTT 
CCAGATTTAA GTTCTGTTCC ACCCCCTCCC CCTTTTCCAG GTGGGGGTCC TCCTCCACCA 
CCTCCTCCTT TTCCTGGGTA TGGAAGCTCA GCAGTTCCTC CACCACTACC TCTACCACTC 
CCCGGGCTTT TTTTTGGGCT GTCAAAACCT CGAAAAGCCC CTGTAGAGCC TGGCTGCCCA 
ATGAAGCCGC TGTATTGGAC AAGGATTCAA CTAAAAAATG GCAGCTCCAG CAATACGTTA 
TGGGAGAACC TAAAGGAGCC CAACATTGCA GACACTAAAG AATTTGAGGA CCTATTTGCA 
AAAGCCACTG TTCAACAGAA GAAGAAACCA CTGTCAGACT CTTATGAGAA GAGAGCAAAA 
GCTAAGCAGG TTATAAAGCT ACTGGATGGG AAGAGGTCTC AGGCTGTGGG CATTTTAATA 
TCCAGTTTAC ATCTGGACAT GAAGGATATT CAACAAGCAA TTCTTAATGT AGATAATTCA 
GTTGTGGACA TAGAAACTCT TGAAGCCTTG TATGAAAACA GGGCTCAGAA GGAGGAGATG 
GAAATCATCA AAAAACACTA TCAGACATCT AAAGCTGAGG ATGTAAAACT GCTGGACAAA 
CCTGAACAGT TTCTTTACGA GCTGTCTCAG ATTCCCAACT TTGTTGAGCG ATCTCAGTGT 
ATTATATTCC AGTCTGTCTT TTTAGAGGGA ATAAGCTCAG TGAGGCGGAA GGTGGATATC 
ATCAGTCGGG CCTGCGATTG CCTCTTGGAG AGGGTTTCTG TGAGAGATAT AATAGGTCTG 
ATTCTTGCAT TCGGAAACTA CATGAATGGA GGCAACCGTA CTCGGGGTCA GGCAGACGGA 
TTTGGGCTGG AAATCCTTCC CAAACTAAAG GATGTTAAAA GCAGGGATAA TAAAGCCAGC 
CTGGTGGATT ATGTTGTACG TTATTATCTA CGATATTTTG ACCAGGATGC TGGAACAGAC 
AAAAGTGTTT TTCCACTGCC AGAATCCCAG GACCTTTTAC TGGCTTCGCA GGTGAAATTT 
GAAGACCTGG ACAAAGACTT ACGGAAACTT AAAAAGGACA TTGAAGTGTG TGAGAAACAG 
TTGAAAGCAG TGGTAAAAGA CTCGCCTAAA GAGAATATCC AGCCATTTAA GGACAAGATG 
TCATCTTTTT TAAACAAAGC CAAAGAAGAA TATAAGACAG AAGAAAGTAA ACTGACCAGA 
TCACAAAACA GGTTTGAAGA AACAATTGGA TATTTTGGAT GTAAGCCAAA GGGCAATGAT 
AAAGAGATCA CCCCCAACTC CTTCTTTGTC TTGTGGTACG AATTCTGCGG TGACTTCAAG 
AGTGTGTGGA AAAGAGAGAG CAAAGTCCTC TCCACGGAAA GGCTTCGCCA GGCTCAGGAG 
TCCGTAAATA AACTGACAGC AGAAAAGAAA GTTGAAACCA GGAAGATTAA CCCTGCAGCA 
AGTCTGAAAG AAAAGCTTCG TCAGAAAGAA GCAAGTGTCA GCACCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fmn1 ( NM_001129929.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123401.2 Xenopus tropicalis formin 1 (fmn1), mRNA.

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CloneID	OXa25292
Clone ID Related Accession (Same CDS sequence)	NM_001129929.2
Accession Version	NM_001129929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	formin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CX802494.1 and BC167459.1. On Oct 5, 2011 this sequence version replaced NM_001129929.1. ##Evidence-Data-START## Transcript exon combination :: BC167459.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028308, SAMD00028309 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGGCA CACACTCCAT CCTCCAATTG CACAAACCCA TTATGGAGCT CTGCTACGTC 
AGCTTCTTCC TTCCCAGAGG TAATGTCAGG GGATTCACAT ACAAACGCTG TGTAACTCGA 
GACAAAGCCA GAACATGTTT TGATAACTGT TATCGAATCA GCGAGGAGAG AGAGACTGCA 
GCACATAAAG ATCCACCTTA TGAAAGATTT ACAGAGGACC TGAAGGAAGC CACAACCCAA 
AACATACTGA CAGAGCTCTA CAAACTCACA GCTGCTAAAG ACAGACTTTT AGTTCAGCTC 
CTAGAAGCCT CCCATAAGCT AGGATCTATA ATGGGAAACC AGGATGGCAA ATTTCAGGAT 
CTTCCGGACG GATATAAATT TCCAGAAGAC GAGGCCCATG GCCATGTGGA TTTCCATGAG 
TCATTTTCAG CTTCAGTGGA GAAGAAGGTG CCTTCCACAA AGGTAAAGAA GAATAGAAGG 
CTTAGTCGGA GGAAAGAAAG TATAGAAGAT TTTGTGAACA AGAAGATAAA GTGGAAAAGT 
AGCACAGAAG CTTCTGCAAC ACAGGTTAGA GACAAAACGT CCTCAGCAAA GGTTTCCCTG 
ACGGGCTCTA CGGATTCATT GTCACGCCCA AAACTGCAGT CTGTGGAGAA CAAGGATAGT 
TACTTACCAG GGCCCATGGT GAAAAATGCA CAGGGTAAGC ACAGTGAAAG CTCAGGGATG 
CTGGGTTCTG AGTCTGCAAT GGGAAGCTTA TTGTCTGTGT ATGATAATGA TGTTTTTGCT 
GATTTTTCTC TGCTGCCTCA CAGTGACAGT CTTTTAAAAG AACTTCAGAG TGCAATTGAC 
ATTATGCCAG AGCAACCAAG CACCCAAGTA CTGGAAGGTT ATATATTTGA AAAGCATTGC 
ACTGAGCATG AGAGCAATGA GCAGGCATCC AGTGGGAGAA GTATAAAGAC AGTTACTGCT 
AAATTGCAGG ACATTAGCAC TTGTGTACAG AAAGTGGTTC ATACTTATAA CTATCAAGAA 
GACGGAGCTG AAGAAAGGAG GTGTGAAAAT ATTACTGAGC TCATTGATGA ACAGAAGGAG 
TTTGTATCAC AGATTGATGT ACCAATGATG TATGAACCAT TACAGTGTCA TATAGAAAAG 
CAGCTATATG GGGGTGTTTA TAATGACAGA CACGGTAGAC TAGAGACATG CAACAATAAT 
ATAGAGCCGC TGCCTTCCTG TGAATATGTC AACAAAAACC TTCTCAGGGT TGTCAAAAGT 
GAAAGCATGG ACGAAACAGA GTATTGGCTG GGACAGATGG ATAGGAGAGG AGAGAAGTGC 
AGTTATGGCC TTAAGCTGCC AGAGAAACTA AGTAAGTCAC AAGAATCATT AAACCATGTA 
ACTGTGCCAC ATAGTGCTCA TATTAAGTTA CAAGACAAAC CTACTGCAGT GAAAACACCA 
ACCTTTGATA CGGCCAGCAT CATGCATATA CCATGTACTA TGTGCAAAGG GATTTCCCCT 
GTTTCTTCTC CAGTATCTAC GCACCTGTCA AGCCCACAGC CCCATCATAG GATTCTCCCC 
TTGCAAACTC AACATGTAGA GGAAGATCTA AAGTTGTGTG GTGTTGAACA TTGGTCTGCC 
CTACCTGATA TTTTCAGAGA ACCATCATCA CTCCAAGAGC TGGGGTATGA TTTTAAAGGA 
TCTACCACGT TCAACATGGA CCCTGATAAC TATCTTTTTG ATGATTTCTT GCAAAATGTG 
AAAGGAAAAA AGACCACATT ACAGCCTAAT CTGCCTCCTC CAGGAGGTGG TGAAGCCTTG 
AAAGATGTTT CCAGAGGTCC AGATACTTCT TCTGGTCTCC AGGCCGGTGT CAGTGAGGAG 
CTGTCAAGTG TTTGTGATAA TAAAACAACT GTCGGGAGGC CCGACCACAG TATTTTGCAT 
CTCAGTCTGG AAATACATAC CGACCTCAGT CCTTCTGATG GAGAAGATCG AACCCCAGGA 
AGACTCCAAG CAGTGTGGCC ACCCCCAAAG CCCAAAGATG AAGAAGAAAA AGTGGGTCTG 
AAATACACAG AAGCAGAGTA TCAGGCTGCT ATCCTGCATT TGAAGAGGGA ACACAAAGAT 
GAGCTAGAGA CACTGAAGTC CCAATCTGAA GTGGCGATCT TCAATGTGCG AGGGGAACAA 
GCAGTTCAGA CAGCAAAATT AGAGGAGGAA ATACAGAAAC TGCAAGCGGA CCTACAGAAT 
AAACTCGGTC ACCAGAATGG GGAGAAAAGG GATGTTTGTG TTTCCACCGA AGATGAGAAT 
TCTCCAAAAT CTTTTCGAAA TGTATGCATC CAAACTGACC GTGAGACTTT TTTGAAACCC 
AATGAGGAGG ACAAATCTCA AAAAAATGAC CCTGTTTTAC CAAAGAAATT AAATATTGCA 
TCAATCAGTC AAAACCTCGC TAGTCCCATA AGTCCCAGCG ATAATTCACA GCACAATTTC 
CCTTTAGCTT CCTTGTCAGT TACTACACCA CCTCCCCACC CCCTCTCTAC ATCTTCACAA 
ACAGCCTCTT CTCCAACACC CTTAGATTGC ACTTCTATTC CTCCACCTCC ATTACTTCCA 
GGTTTCCCTT CTGTTCCACC ACCTCCACCT CTTCCTGGTT CTAGCTCTGT TCCACCTCCT 
CCACCTCTTC CAGGTATCAG TTCTGCCCCA CCACCTCCCC CTCTTCCAGG TTTTAGCTCT 
GTTCCACCTC CTCCTCCTCC TCTTCCAGAT TTAAGTTCTG TTCCACCCCC TCCCCCTTTT 
CCAGGTGGGG GTCCTCCTCC ACCACCTCCT CCTTTTCCTG GGTATGGAAG CTCAGCAGTT 
CCTCCACCAC TACCTCTACC ACTCCCCGGG CTTTTTTTTG GGCTGTCAAA ACCTCGAAAA 
GCCCCTGTAG AGCCTGGCTG CCCAATGAAG CCGCTGTATT GGACAAGGAT TCAACTAAAA 
AATGGCAGCT CCAGCAATAC GTTATGGGAG AACCTAAAGG AGCCCAACAT TGCAGACACT 
AAAGAATTTG AGGACCTATT TGCAAAAGCC ACTGTTCAAC AGAAGAAGAA ACCACTGTCA 
GACTCTTATG AGAAGAGAGC AAAAGCTAAG CAGGTTATAA AGCTACTGGA TGGGAAGAGG 
TCTCAGGCTG TGGGCATTTT AATATCCAGT TTACATCTGG ACATGAAGGA TATTCAACAA 
GCAATTCTTA ATGTAGATAA TTCAGTTGTG GACATAGAAA CTCTTGAAGC CTTGTATGAA 
AACAGGGCTC AGAAGGAGGA GATGGAAATC ATCAAAAAAC ACTATCAGAC ATCTAAAGCT 
GAGGATGTAA AACTGCTGGA CAAACCTGAA CAGTTTCTTT ACGAGCTGTC TCAGATTCCC 
AACTTTGTTG AGCGATCTCA GTGTATTATA TTCCAGTCTG TCTTTTTAGA GGGAATAAGC 
TCAGTGAGGC GGAAGGTGGA TATCATCAGT CGGGCCTGCG ATTGCCTCTT GGAGAGGGTT 
TCTGTGAGAG ATATAATAGG TCTGATTCTT GCATTCGGAA ACTACATGAA TGGAGGCAAC 
CGTACTCGGG GTCAGGCAGA CGGATTTGGG CTGGAAATCC TTCCCAAACT AAAGGATGTT 
AAAAGCAGGG ATAATAAAGC CAGCCTGGTG GATTATGTTG TACGTTATTA TCTACGATAT 
TTTGACCAGG ATGCTGGAAC AGACAAAAGT GTTTTTCCAC TGCCAGAATC CCAGGACCTT 
TTACTGGCTT CGCAGGTGAA ATTTGAAGAC CTGGACAAAG ACTTACGGAA ACTTAAAAAG 
GACATTGAAG TGTGTGAGAA ACAGTTGAAA GCAGTGGTAA AAGACTCGCC TAAAGAGAAT 
ATCCAGCCAT TTAAGGACAA GATGTCATCT TTTTTAAACA AAGCCAAAGA AGAATATAAG 
ACAGAAGAAA GTAAACTGAC CAGATCACAA AACAGGTTTG AAGAAACAAT TGGATATTTT 
GGATGTAAGC CAAAGGGCAA TGATAAAGAG ATCACCCCCA ACTCCTTCTT TGTCTTGTGG 
TACGAATTCT GCGGTGACTT CAAGAGTGTG TGGAAAAGAG AGAGCAAAGT CCTCTCCACG 
GAAAGGCTTC GCCAGGCTCA GGAGTCCGTA AATAAACTGA CAGCAGAAAA GAAAGTTGAA 
ACCAGGAAGA TTAACCCTGC AGCAAGTCTG AAAGAAAAGC TTCGTCAGAA AGAAGCAAGT 
GTCAGCACCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123401.2
CDS	325..4539
Translation	MEGTHSILQLHKPIMELCYVSFFLPRGNVRGFTYKRCVTRDKARTCFDNCYRISEERETAAHKDPPYERFTEDLKEATTQNILTELYKLTAAKDRLLVQLLEASHKLGSIMGNQDGKFQDLPDGYKFPEDEAHGHVDFHESFSASVEKKVPSTKVKKNRRLSRRKESIEDFVNKKIKWKSSTEASATQVRDKTSSAKVSLTGSTDSLSRPKLQSVENKDSYLPGPMVKNAQGKHSESSGMLGSESAMGSLLSVYDNDVFADFSLLPHSDSLLKELQSAIDIMPEQPSTQVLEGYIFEKHCTEHESNEQASSGRSIKTVTAKLQDISTCVQKVVHTYNYQEDGAEERRCENITELIDEQKEFVSQIDVPMMYEPLQCHIEKQLYGGVYNDRHGRLETCNNNIEPLPSCEYVNKNLLRVVKSESMDETEYWLGQMDRRGEKCSYGLKLPEKLSKSQESLNHVTVPHSAHIKLQDKPTAVKTPTFDTASIMHIPCTMCKGISPVSSPVSTHLSSPQPHHRILPLQTQHVEEDLKLCGVEHWSALPDIFREPSSLQELGYDFKGSTTFNMDPDNYLFDDFLQNVKGKKTTLQPNLPPPGGGEALKDVSRGPDTSSGLQAGVSEELSSVCDNKTTVGRPDHSILHLSLEIHTDLSPSDGEDRTPGRLQAVWPPPKPKDEEEKVGLKYTEAEYQAAILHLKREHKDELETLKSQSEVAIFNVRGEQAVQTAKLEEEIQKLQADLQNKLGHQNGEKRDVCVSTEDENSPKSFRNVCIQTDRETFLKPNEEDKSQKNDPVLPKKLNIASISQNLASPISPSDNSQHNFPLASLSVTTPPPHPLSTSSQTASSPTPLDCTSIPPPPLLPGFPSVPPPPPLPGSSSVPPPPPLPGISSAPPPPPLPGFSSVPPPPPPLPDLSSVPPPPPFPGGGPPPPPPPFPGYGSSAVPPPLPLPLPGLFFGLSKPRKAPVEPGCPMKPLYWTRIQLKNGSSSNTLWENLKEPNIADTKEFEDLFAKATVQQKKKPLSDSYEKRAKAKQVIKLLDGKRSQAVGILISSLHLDMKDIQQAILNVDNSVVDIETLEALYENRAQKEEMEIIKKHYQTSKAEDVKLLDKPEQFLYELSQIPNFVERSQCIIFQSVFLEGISSVRRKVDIISRACDCLLERVSVRDIIGLILAFGNYMNGGNRTRGQADGFGLEILPKLKDVKSRDNKASLVDYVVRYYLRYFDQDAGTDKSVFPLPESQDLLLASQVKFEDLDKDLRKLKKDIEVCEKQLKAVVKDSPKENIQPFKDKMSSFLNKAKEEYKTEESKLTRSQNRFEETIGYFGCKPKGNDKEITPNSFFVLWYEFCGDFKSVWKRESKVLSTERLRQAQESVNKLTAEKKVETRKINPAASLKEKLRQKEASVSTN

Target ORF information:

RefSeq Version	NM_001129929.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis formin 1 (fmn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129929.2

ORF Insert Sequence:

ATGGAAGGCA CACACTCCAT CCTCCAATTG CACAAACCCA TTATGGAGCT CTGCTACGTC 
AGCTTCTTCC TTCCCAGAGG TAATGTCAGG GGATTCACAT ACAAACGCTG TGTAACTCGA 
GACAAAGCCA GAACATGTTT TGATAACTGT TATCGAATCA GCGAGGAGAG AGAGACTGCA 
GCACATAAAG ATCCACCTTA TGAAAGATTT ACAGAGGACC TGAAGGAAGC CACAACCCAA 
AACATACTGA CAGAGCTCTA CAAACTCACA GCTGCTAAAG ACAGACTTTT AGTTCAGCTC 
CTAGAAGCCT CCCATAAGCT AGGATCTATA ATGGGAAACC AGGATGGCAA ATTTCAGGAT 
CTTCCGGACG GATATAAATT TCCAGAAGAC GAGGCCCATG GCCATGTGGA TTTCCATGAG 
TCATTTTCAG CTTCAGTGGA GAAGAAGGTG CCTTCCACAA AGGTAAAGAA GAATAGAAGG 
CTTAGTCGGA GGAAAGAAAG TATAGAAGAT TTTGTGAACA AGAAGATAAA GTGGAAAAGT 
AGCACAGAAG CTTCTGCAAC ACAGGTTAGA GACAAAACGT CCTCAGCAAA GGTTTCCCTG 
ACGGGCTCTA CGGATTCATT GTCACGCCCA AAACTGCAGT CTGTGGAGAA CAAGGATAGT 
TACTTACCAG GGCCCATGGT GAAAAATGCA CAGGGTAAGC ACAGTGAAAG CTCAGGGATG 
CTGGGTTCTG AGTCTGCAAT GGGAAGCTTA TTGTCTGTGT ATGATAATGA TGTTTTTGCT 
GATTTTTCTC TGCTGCCTCA CAGTGACAGT CTTTTAAAAG AACTTCAGAG TGCAATTGAC 
ATTATGCCAG AGCAACCAAG CACCCAAGTA CTGGAAGGTT ATATATTTGA AAAGCATTGC 
ACTGAGCATG AGAGCAATGA GCAGGCATCC AGTGGGAGAA GTATAAAGAC AGTTACTGCT 
AAATTGCAGG ACATTAGCAC TTGTGTACAG AAAGTGGTTC ATACTTATAA CTATCAAGAA 
GACGGAGCTG AAGAAAGGAG GTGTGAAAAT ATTACTGAGC TCATTGATGA ACAGAAGGAG 
TTTGTATCAC AGATTGATGT ACCAATGATG TATGAACCAT TACAGTGTCA TATAGAAAAG 
CAGCTATATG GGGGTGTTTA TAATGACAGA CACGGTAGAC TAGAGACATG CAACAATAAT 
ATAGAGCCGC TGCCTTCCTG TGAATATGTC AACAAAAACC TTCTCAGGGT TGTCAAAAGT 
GAAAGCATGG ACGAAACAGA GTATTGGCTG GGACAGATGG ATAGGAGAGG AGAGAAGTGC 
AGTTATGGCC TTAAGCTGCC AGAGAAACTA AGTAAGTCAC AAGAATCATT AAACCATGTA 
ACTGTGCCAC ATAGTGCTCA TATTAAGTTA CAAGACAAAC CTACTGCAGT GAAAACACCA 
ACCTTTGATA CGGCCAGCAT CATGCATATA CCATGTACTA TGTGCAAAGG GATTTCCCCT 
GTTTCTTCTC CAGTATCTAC GCACCTGTCA AGCCCACAGC CCCATCATAG GATTCTCCCC 
TTGCAAACTC AACATGTAGA GGAAGATCTA AAGTTGTGTG GTGTTGAACA TTGGTCTGCC 
CTACCTGATA TTTTCAGAGA ACCATCATCA CTCCAAGAGC TGGGGTATGA TTTTAAAGGA 
TCTACCACGT TCAACATGGA CCCTGATAAC TATCTTTTTG ATGATTTCTT GCAAAATGTG 
AAAGGAAAAA AGACCACATT ACAGCCTAAT CTGCCTCCTC CAGGAGGTGG TGAAGCCTTG 
AAAGATGTTT CCAGAGGTCC AGATACTTCT TCTGGTCTCC AGGCCGGTGT CAGTGAGGAG 
CTGTCAAGTG TTTGTGATAA TAAAACAACT GTCGGGAGGC CCGACCACAG TATTTTGCAT 
CTCAGTCTGG AAATACATAC CGACCTCAGT CCTTCTGATG GAGAAGATCG AACCCCAGGA 
AGACTCCAAG CAGTGTGGCC ACCCCCAAAG CCCAAAGATG AAGAAGAAAA AGTGGGTCTG 
AAATACACAG AAGCAGAGTA TCAGGCTGCT ATCCTGCATT TGAAGAGGGA ACACAAAGAT 
GAGCTAGAGA CACTGAAGTC CCAATCTGAA GTGGCGATCT TCAATGTGCG AGGGGAACAA 
GCAGTTCAGA CAGCAAAATT AGAGGAGGAA ATACAGAAAC TGCAAGCGGA CCTACAGAAT 
AAACTCGGTC ACCAGAATGG GGAGAAAAGG GATGTTTGTG TTTCCACCGA AGATGAGAAT 
TCTCCAAAAT CTTTTCGAAA TGTATGCATC CAAACTGACC GTGAGACTTT TTTGAAACCC 
AATGAGGAGG ACAAATCTCA AAAAAATGAC CCTGTTTTAC CAAAGAAATT AAATATTGCA 
TCAATCAGTC AAAACCTCGC TAGTCCCATA AGTCCCAGCG ATAATTCACA GCACAATTTC 
CCTTTAGCTT CCTTGTCAGT TACTACACCA CCTCCCCACC CCCTCTCTAC ATCTTCACAA 
ACAGCCTCTT CTCCAACACC CTTAGATTGC ACTTCTATTC CTCCACCTCC ATTACTTCCA 
GGTTTCCCTT CTGTTCCACC ACCTCCACCT CTTCCTGGTT CTAGCTCTGT TCCACCTCCT 
CCACCTCTTC CAGGTATCAG TTCTGCCCCA CCACCTCCCC CTCTTCCAGG TTTTAGCTCT 
GTTCCACCTC CTCCTCCTCC TCTTCCAGAT TTAAGTTCTG TTCCACCCCC TCCCCCTTTT 
CCAGGTGGGG GTCCTCCTCC ACCACCTCCT CCTTTTCCTG GGTATGGAAG CTCAGCAGTT 
CCTCCACCAC TACCTCTACC ACTCCCCGGG CTTTTTTTTG GGCTGTCAAA ACCTCGAAAA 
GCCCCTGTAG AGCCTGGCTG CCCAATGAAG CCGCTGTATT GGACAAGGAT TCAACTAAAA 
AATGGCAGCT CCAGCAATAC GTTATGGGAG AACCTAAAGG AGCCCAACAT TGCAGACACT 
AAAGAATTTG AGGACCTATT TGCAAAAGCC ACTGTTCAAC AGAAGAAGAA ACCACTGTCA 
GACTCTTATG AGAAGAGAGC AAAAGCTAAG CAGGTTATAA AGCTACTGGA TGGGAAGAGG 
TCTCAGGCTG TGGGCATTTT AATATCCAGT TTACATCTGG ACATGAAGGA TATTCAACAA 
GCAATTCTTA ATGTAGATAA TTCAGTTGTG GACATAGAAA CTCTTGAAGC CTTGTATGAA 
AACAGGGCTC AGAAGGAGGA GATGGAAATC ATCAAAAAAC ACTATCAGAC ATCTAAAGCT 
GAGGATGTAA AACTGCTGGA CAAACCTGAA CAGTTTCTTT ACGAGCTGTC TCAGATTCCC 
AACTTTGTTG AGCGATCTCA GTGTATTATA TTCCAGTCTG TCTTTTTAGA GGGAATAAGC 
TCAGTGAGGC GGAAGGTGGA TATCATCAGT CGGGCCTGCG ATTGCCTCTT GGAGAGGGTT 
TCTGTGAGAG ATATAATAGG TCTGATTCTT GCATTCGGAA ACTACATGAA TGGAGGCAAC 
CGTACTCGGG GTCAGGCAGA CGGATTTGGG CTGGAAATCC TTCCCAAACT AAAGGATGTT 
AAAAGCAGGG ATAATAAAGC CAGCCTGGTG GATTATGTTG TACGTTATTA TCTACGATAT 
TTTGACCAGG ATGCTGGAAC AGACAAAAGT GTTTTTCCAC TGCCAGAATC CCAGGACCTT 
TTACTGGCTT CGCAGGTGAA ATTTGAAGAC CTGGACAAAG ACTTACGGAA ACTTAAAAAG 
GACATTGAAG TGTGTGAGAA ACAGTTGAAA GCAGTGGTAA AAGACTCGCC TAAAGAGAAT 
ATCCAGCCAT TTAAGGACAA GATGTCATCT TTTTTAAACA AAGCCAAAGA AGAATATAAG 
ACAGAAGAAA GTAAACTGAC CAGATCACAA AACAGGTTTG AAGAAACAAT TGGATATTTT 
GGATGTAAGC CAAAGGGCAA TGATAAAGAG ATCACCCCCA ACTCCTTCTT TGTCTTGTGG 
TACGAATTCT GCGGTGACTT CAAGAGTGTG TGGAAAAGAG AGAGCAAAGT CCTCTCCACG 
GAAAGGCTTC GCCAGGCTCA GGAGTCCGTA AATAAACTGA CAGCAGAAAA GAAAGTTGAA 
ACCAGGAAGA TTAACCCTGC AGCAAGTCTG AAAGAAAAGC TTCGTCAGAA AGAAGCAAGT 
GTCAGCACCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fmn1 ( XM_012968078.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823532.1 Xenopus tropicalis formin 1 (fmn1), transcript variant X1, mRNA.

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CloneID	OXa32276
Clone ID Related Accession (Same CDS sequence)	XM_012968078.2
Accession Version	XM_012968078.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	formin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968078.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGA AAGAGACTGC TACAAATATG CCAGAGGCGG CAGCTTCTGC TTCAGTAGGG 
AGACCTGATT CATCAGGTCT GTCTATACTG AGTAAATTTT CCATAAAGTC ATTTTTTGGA 
ATCACAACAA GGTTAGACAG CCAGAAAAGC AATGAAGGGA ATGCTGTTTT AAAATCTTTT 
AAATTACTTG GAAATAAATC AGAGTTACAG ACAGACCCTG CGAGCAGTGC ACAGAATTCA 
AGCTGTGAGA GGTCAGTACC ACATTCTGAC ATAACAGGGT GGGAGGCAAA GGACCCTGAA 
GAGCCAAAGG CAACCACTGA TAAACATGTA CAAATTGGAC AGGCGGAAGG CTCAGATCTG 
GATTTGCAAC AGCACAGTTT AAAGGATCTA AGTTGTGAGA ATGAGGGGGA TTCACAAGTC 
ACAGTAGGAC AGCCTGCAGT AGGTGACATT GAAAAGGACA AAGAAAACGC TGAGAAGGAT 
AACCTGGGAG GTGCAGATGT ACTGTTTTTA GATGAGCAAA AGCATGAAAA AAAGGGAGTG 
TGCACAGAAA GTCTGACCGT TTTAGGCACT TGTAAGGATG AAAAAGCTGA TACACATTCC 
AGTGCAGAGC ACGCAGCCTG TGATGTTCCC AGCACAGGAG TTAATGACAA GGATGATTTG 
CTTGTACGTG GTATTCTTGT ACAGACAACA AGTGATACAG ACTCTGACGG TGAGGATACT 
GATCAAGTCA CAGACCAAAA GAGTGCTCCC ATGTCCAAGA ATTTATCGGC ATCTCATGTG 
TCAGTAAACA ATAGTCCCAA ACAGGAGCCA GAAGCAACAG GTCCAGGAAA GACAGGGTAC 
ATGGAGGGAG AAGACAAAGA ACAAGTGAAT ATAAACAGCT CAGGCTCTTT GGGGGCCCAT 
ATGGATGCAC AACAAGGGAT TACACAGGAG GCAGAAATTA ACAGTCCTGA AGACAATTCC 
GTTCCAGAAT CTGTAACAGC TGCATCCCAT TCTGAGCCAC AGAATGAGAA GACCTATGTG 
CTTCCAGCAA TTTTTAGTGG TCTGCGTGTA CACAAGAAAG GAGCACCGGT GGATGATAGG 
GAGATGGTTA CTGTAAAACC AGGTGACAGT GATTTGGCAT TACTTAAATT GTCCCAGACA 
GCTCAAAAAT CCAAAGTCTC CATTGAAACA ATAAGAAAGC GAGAGCAGAA GAAGCCAACA 
GAAACCAGAC CAAGTTCAAA GTTCATGGAA CAACTTTCCC AGCTACTGAG CTTTGATAAC 
CCCAAGCAAG ATGAAACACA GGGGGGATCT ACAGAAGAAC AAAGTATTTC AAATGGCACT 
GAGCAGGACC TCCAGGCTGT TAATGAGGAC AAATTGGAAA CTAGCTCACA AGAGACTGCA 
ATGGATGCTT TCAAATCCTT TTTCATGATT GCTCCCAAAA AGACAATAAC ACAGGATTCC 
CATGACCTGG AGGCTGTCAA GCGCAAGCAA AAGAGTGAGA AGGATACACT GAAGGCTATC 
TTTGAGAAGC CTAGACCCAC AGACCTTGAC CTGGCATCTG ACGAAAAGAA AAATACCGAC 
CTCAGTCCTT CTGATGGAGA AGATCGAACC CCAGGAAGAC TCCAAGCAGT GTGGCCACCC 
CCAAAGCCCA AAGATGAAGA AGAAAAAGTG GGTCTGAAAT ACACAGAAGC AGAGTATCAG 
GCTGCTATCC TGCATTTGAA GAGGGAACAC AAAGATGAGC TAGAGACACT GAAGTCCCAA 
TCTGAAGTGG CGATCTTCAA TGTGCGAGGG GAACAAGCAG TTCAGACAGC AAAATTAGAG 
GAGGAAATAC AGAAACTGCA AGCGGACCTA CAGAATAAAC TCAGTCACCA GAATGGGGAG 
AAAAGGGATG TTTGTGTTTC CACCGAAGAT GAGAATTCTC CAAAATCTTT TCGAAATGTA 
TGCATCCAAA CTGACCGTGA GACTTTTTTG AAACCCAATG AGGAGGACAA ATCTCAAAAA 
AATGACACTG TTTTACCAAA GAAATTAAAT ATTGCATCAA TCAGTCAAAA CCTCGCTAGT 
CCCATAAGTC CCAGCGATAA TTCACAGCAC AATTTCCCTT TAGCTTCCTT GTCAGTTACT 
ACACCACCTC CCCACCCCCT CTCTACATCT TCACAAACAG CCTCTTCTCC AACACCCTTA 
GATTGCACTT CTATTCCTCC ACCTCCATTA CTTCCAGGTT TCCCTTCTGT TCCACCACCT 
CCACCTCTTC CTGGTTCTAG CTCTGTTCCA CCTCCTCCAC CTCTTCCAGG TATCAGTTCT 
GCCCCACCAC CTCCCCCTCT TCCAGGTTTT AGCTCTGTTC CACCTCCTCC TCCTCTTCCA 
GATTTAAGTT CTGTTCCACC CCCTCCCCCT TTTCCAGGTG GGGGTCCTCC TCCACCACCT 
CCTCCTTTTC CTGGGTATGG AAGCTCAGCA GTTCCTCCAC CACTACCTCT ACCACTCCCC 
GGGCTTTTTT TTGGGCTGTC AAAACCTCGA AAAGCCCCTG TAGAGCCTGG CTGCCCAATG 
AAGCCGCTGT ATTGGACAAG GATTCAACTA AAAAATGGCA GCTCCAGCAA TACGTTATGG 
GAGAACCTAA AGGAGCCCAA CATTGCAGAC ACTAAAGAAT TTGAGGACTT ATTTGCAAAA 
GCCACTGTTC AACAGAAGAA GAAACCACTG TCAGACTCTT ATGAGAAGAG AGCAAAAGCT 
AAGCAGGTTA TAAAGCTACT GGATGGGAAG AGGTCTCAGG CTGTGGGCAT TTTAATATCC 
AGTTTACATC TGGACATGAA GGATATTCAA CAAGCAATTC TTAATGTAGA TAATTCAGTT 
GTGGACATAG AAACTCTTGA AGCCTTGTAT GAAAACAGGG CTCAGAAGGA GGAGATGGAA 
ATCATCAAAA AACACTATCA GACATCTAAA GCTGAGGATG TAAAACTGCT GGACAAACCT 
GAACAGTTTC TTTACGAGCT GTCTCAGATT CCCAACTTTG TTGAGCGATC TCAGTGTATT 
ATATTCCAGT CTGTCTTTTT AGAGGGAATA AGCTCAGTGA GGCGGAAGGT GGATATCATC 
AGTCGGGCCT GCGATTGCCT CTTGGAGAGG GTTTCTGTGA GAGATATAAT AGGTCTGATT 
CTTGCATTCG GAAACTACAT GAATGGAGGC AACCGTACTC GGGGTCAGGC AGACGGATTT 
GGGCTGGAAA TCCTTCCCAA ACTAAAGGAT GTTAAAAGCA GGGATAATAA AGCCAGCCTG 
GTGGATTATG TTGTACGTTA TTATCTACGA TATTTTGACC AGGATGCTGG AACAGACAAA 
AGTGTTTTTC CACTGCCAGA ATCCCAGGAC CTTTTACTGG CTTCGCAGGT GAAATTTGAA 
GACCTGGACA AAGACTTACG GAAACTTAAA AAGGACATTG AAGTGTGTGA GAAACAGTTG 
AAAGCAGTGG TAAAAGACTC GCCTAAAGAG AATATCCAGC CATTTAAGGA CAAGATGTCA 
TCTTTTTTAA ACAAAGCCAA AGAAGAATAT AAGACAGAAG AAAGTAAACT GACCAGATCA 
CAAAACAGGT TTGAAGAAAC AATTGGATAT TTTGGATGTA AGCCAAAGGG CAATGATAAA 
GAGATCACCC CCAACTCCTT CTTTGTCTTG TGGTACGAAT TCTGCGGTGA CTTCAAGAGT 
GTGTGGAAAA GAGAGAGCAA AGTCCTCTCC ACGGAAAGGC GCCTTTGTTT TCTTTTCGGA 
TCAAATGGAC ATCAAGGGAA GGTGATTCCC CCCCAGGGCT CCAGAGGGAA CCCGCTAGCG 
GCCGTCTGTC CTCACGAAAA GATAAACACG CCAGATCTGA TAAGTTACAC TTTTCTTTAC 
CACTCCCAAT TACTTGCTAG TAAAATGAAA GGAGACCTCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823532.1
CDS	748..4632
Translation	MELKETATNMPEAAASASVGRPDSSGLSILSKFSIKSFFGITTRLDSQKSNEGNAVLKSFKLLGNKSELQTDPASSAQNSSCERSVPHSDITGWEAKDPEEPKATTDKHVQIGQAEGSDLDLQQHSLKDLSCENEGDSQVTVGQPAVGDIEKDKENAEKDNLGGADVLFLDEQKHEKKGVCTESLTVLGTCKDEKADTHSSAEHAACDVPSTGVNDKDDLLVRGILVQTTSDTDSDGEDTDQVTDQKSAPMSKNLSASHVSVNNSPKQEPEATGPGKTGYMEGEDKEQVNINSSGSLGAHMDAQQGITQEAEINSPEDNSVPESVTAASHSEPQNEKTYVLPAIFSGLRVHKKGAPVDDREMVTVKPGDSDLALLKLSQTAQKSKVSIETIRKREQKKPTETRPSSKFMEQLSQLLSFDNPKQDETQGGSTEEQSISNGTEQDLQAVNEDKLETSSQETAMDAFKSFFMIAPKKTITQDSHDLEAVKRKQKSEKDTLKAIFEKPRPTDLDLASDEKKNTDLSPSDGEDRTPGRLQAVWPPPKPKDEEEKVGLKYTEAEYQAAILHLKREHKDELETLKSQSEVAIFNVRGEQAVQTAKLEEEIQKLQADLQNKLSHQNGEKRDVCVSTEDENSPKSFRNVCIQTDRETFLKPNEEDKSQKNDTVLPKKLNIASISQNLASPISPSDNSQHNFPLASLSVTTPPPHPLSTSSQTASSPTPLDCTSIPPPPLLPGFPSVPPPPPLPGSSSVPPPPPLPGISSAPPPPPLPGFSSVPPPPPLPDLSSVPPPPPFPGGGPPPPPPPFPGYGSSAVPPPLPLPLPGLFFGLSKPRKAPVEPGCPMKPLYWTRIQLKNGSSSNTLWENLKEPNIADTKEFEDLFAKATVQQKKKPLSDSYEKRAKAKQVIKLLDGKRSQAVGILISSLHLDMKDIQQAILNVDNSVVDIETLEALYENRAQKEEMEIIKKHYQTSKAEDVKLLDKPEQFLYELSQIPNFVERSQCIIFQSVFLEGISSVRRKVDIISRACDCLLERVSVRDIIGLILAFGNYMNGGNRTRGQADGFGLEILPKLKDVKSRDNKASLVDYVVRYYLRYFDQDAGTDKSVFPLPESQDLLLASQVKFEDLDKDLRKLKKDIEVCEKQLKAVVKDSPKENIQPFKDKMSSFLNKAKEEYKTEESKLTRSQNRFEETIGYFGCKPKGNDKEITPNSFFVLWYEFCGDFKSVWKRESKVLSTERRLCFLFGSNGHQGKVIPPQGSRGNPLAAVCPHEKINTPDLISYTFLYHSQLLASKMKGDLS

Target ORF information:

RefSeq Version	XM_012968078.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis formin 1 (fmn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968078.2

ORF Insert Sequence:

ATGGAGCTGA AAGAGACTGC TACAAATATG CCAGAGGCGG CAGCTTCTGC TTCAGTAGGG 
AGACCTGATT CATCAGGTCT GTCTATACTG AGTAAATTTT CCATAAAGTC ATTTTTTGGA 
ATCACAACAA GGTTAGACAG CCAGAAAAGC AATGAAGGGA ATGCTGTTTT AAAATCTTTT 
AAATTACTTG GAAATAAATC AGAGTTACAG ACAGACCCTG CGAGCAGTGC ACAGAATTCA 
AGCTGTGAGA GGTCAGTACC ACATTCTGAC ATAACAGGGT GGGAGGCAAA GGACCCTGAA 
GAGCCAAAGG CAACCACTGA TAAACATGTA CAAATTGGAC AGGCGGAAGG CTCAGATCTG 
GATTTGCAAC AGCACAGTTT AAAGGATCTA AGTTGTGAGA ATGAGGGGGA TTCACAAGTC 
ACAGTAGGAC AGCCTGCAGT AGGTGACATT GAAAAGGACA AAGAAAACGC TGAGAAGGAT 
AACCTGGGAG GTGCAGATGT ACTGTTTTTA GATGAGCAAA AGCATGAAAA AAAGGGAGTG 
TGCACAGAAA GTCTGACCGT TTTAGGCACT TGTAAGGATG AAAAAGCTGA TACACATTCC 
AGTGCAGAGC ACGCAGCCTG TGATGTTCCC AGCACAGGAG TTAATGACAA GGATGATTTG 
CTTGTACGTG GTATTCTTGT ACAGACAACA AGTGATACAG ACTCTGACGG TGAGGATACT 
GATCAAGTCA CAGACCAAAA GAGTGCTCCC ATGTCCAAGA ATTTATCGGC ATCTCATGTG 
TCAGTAAACA ATAGTCCCAA ACAGGAGCCA GAAGCAACAG GTCCAGGAAA GACAGGGTAC 
ATGGAGGGAG AAGACAAAGA ACAAGTGAAT ATAAACAGCT CAGGCTCTTT GGGGGCCCAT 
ATGGATGCAC AACAAGGGAT TACACAGGAG GCAGAAATTA ACAGTCCTGA AGACAATTCC 
GTTCCAGAAT CTGTAACAGC TGCATCCCAT TCTGAGCCAC AGAATGAGAA GACCTATGTG 
CTTCCAGCAA TTTTTAGTGG TCTGCGTGTA CACAAGAAAG GAGCACCGGT GGATGATAGG 
GAGATGGTTA CTGTAAAACC AGGTGACAGT GATTTGGCAT TACTTAAATT GTCCCAGACA 
GCTCAAAAAT CCAAAGTCTC CATTGAAACA ATAAGAAAGC GAGAGCAGAA GAAGCCAACA 
GAAACCAGAC CAAGTTCAAA GTTCATGGAA CAACTTTCCC AGCTACTGAG CTTTGATAAC 
CCCAAGCAAG ATGAAACACA GGGGGGATCT ACAGAAGAAC AAAGTATTTC AAATGGCACT 
GAGCAGGACC TCCAGGCTGT TAATGAGGAC AAATTGGAAA CTAGCTCACA AGAGACTGCA 
ATGGATGCTT TCAAATCCTT TTTCATGATT GCTCCCAAAA AGACAATAAC ACAGGATTCC 
CATGACCTGG AGGCTGTCAA GCGCAAGCAA AAGAGTGAGA AGGATACACT GAAGGCTATC 
TTTGAGAAGC CTAGACCCAC AGACCTTGAC CTGGCATCTG ACGAAAAGAA AAATACCGAC 
CTCAGTCCTT CTGATGGAGA AGATCGAACC CCAGGAAGAC TCCAAGCAGT GTGGCCACCC 
CCAAAGCCCA AAGATGAAGA AGAAAAAGTG GGTCTGAAAT ACACAGAAGC AGAGTATCAG 
GCTGCTATCC TGCATTTGAA GAGGGAACAC AAAGATGAGC TAGAGACACT GAAGTCCCAA 
TCTGAAGTGG CGATCTTCAA TGTGCGAGGG GAACAAGCAG TTCAGACAGC AAAATTAGAG 
GAGGAAATAC AGAAACTGCA AGCGGACCTA CAGAATAAAC TCAGTCACCA GAATGGGGAG 
AAAAGGGATG TTTGTGTTTC CACCGAAGAT GAGAATTCTC CAAAATCTTT TCGAAATGTA 
TGCATCCAAA CTGACCGTGA GACTTTTTTG AAACCCAATG AGGAGGACAA ATCTCAAAAA 
AATGACACTG TTTTACCAAA GAAATTAAAT ATTGCATCAA TCAGTCAAAA CCTCGCTAGT 
CCCATAAGTC CCAGCGATAA TTCACAGCAC AATTTCCCTT TAGCTTCCTT GTCAGTTACT 
ACACCACCTC CCCACCCCCT CTCTACATCT TCACAAACAG CCTCTTCTCC AACACCCTTA 
GATTGCACTT CTATTCCTCC ACCTCCATTA CTTCCAGGTT TCCCTTCTGT TCCACCACCT 
CCACCTCTTC CTGGTTCTAG CTCTGTTCCA CCTCCTCCAC CTCTTCCAGG TATCAGTTCT 
GCCCCACCAC CTCCCCCTCT TCCAGGTTTT AGCTCTGTTC CACCTCCTCC TCCTCTTCCA 
GATTTAAGTT CTGTTCCACC CCCTCCCCCT TTTCCAGGTG GGGGTCCTCC TCCACCACCT 
CCTCCTTTTC CTGGGTATGG AAGCTCAGCA GTTCCTCCAC CACTACCTCT ACCACTCCCC 
GGGCTTTTTT TTGGGCTGTC AAAACCTCGA AAAGCCCCTG TAGAGCCTGG CTGCCCAATG 
AAGCCGCTGT ATTGGACAAG GATTCAACTA AAAAATGGCA GCTCCAGCAA TACGTTATGG 
GAGAACCTAA AGGAGCCCAA CATTGCAGAC ACTAAAGAAT TTGAGGACTT ATTTGCAAAA 
GCCACTGTTC AACAGAAGAA GAAACCACTG TCAGACTCTT ATGAGAAGAG AGCAAAAGCT 
AAGCAGGTTA TAAAGCTACT GGATGGGAAG AGGTCTCAGG CTGTGGGCAT TTTAATATCC 
AGTTTACATC TGGACATGAA GGATATTCAA CAAGCAATTC TTAATGTAGA TAATTCAGTT 
GTGGACATAG AAACTCTTGA AGCCTTGTAT GAAAACAGGG CTCAGAAGGA GGAGATGGAA 
ATCATCAAAA AACACTATCA GACATCTAAA GCTGAGGATG TAAAACTGCT GGACAAACCT 
GAACAGTTTC TTTACGAGCT GTCTCAGATT CCCAACTTTG TTGAGCGATC TCAGTGTATT 
ATATTCCAGT CTGTCTTTTT AGAGGGAATA AGCTCAGTGA GGCGGAAGGT GGATATCATC 
AGTCGGGCCT GCGATTGCCT CTTGGAGAGG GTTTCTGTGA GAGATATAAT AGGTCTGATT 
CTTGCATTCG GAAACTACAT GAATGGAGGC AACCGTACTC GGGGTCAGGC AGACGGATTT 
GGGCTGGAAA TCCTTCCCAA ACTAAAGGAT GTTAAAAGCA GGGATAATAA AGCCAGCCTG 
GTGGATTATG TTGTACGTTA TTATCTACGA TATTTTGACC AGGATGCTGG AACAGACAAA 
AGTGTTTTTC CACTGCCAGA ATCCCAGGAC CTTTTACTGG CTTCGCAGGT GAAATTTGAA 
GACCTGGACA AAGACTTACG GAAACTTAAA AAGGACATTG AAGTGTGTGA GAAACAGTTG 
AAAGCAGTGG TAAAAGACTC GCCTAAAGAG AATATCCAGC CATTTAAGGA CAAGATGTCA 
TCTTTTTTAA ACAAAGCCAA AGAAGAATAT AAGACAGAAG AAAGTAAACT GACCAGATCA 
CAAAACAGGT TTGAAGAAAC AATTGGATAT TTTGGATGTA AGCCAAAGGG CAATGATAAA 
GAGATCACCC CCAACTCCTT CTTTGTCTTG TGGTACGAAT TCTGCGGTGA CTTCAAGAGT 
GTGTGGAAAA GAGAGAGCAA AGTCCTCTCC ACGGAAAGGC GCCTTTGTTT TCTTTTCGGA 
TCAAATGGAC ATCAAGGGAA GGTGATTCCC CCCCAGGGCT CCAGAGGGAA CCCGCTAGCG 
GCCGTCTGTC CTCACGAAAA GATAAACACG CCAGATCTGA TAAGTTACAC TTTTCTTTAC 
CACTCCCAAT TACTTGCTAG TAAAATGAAA GGAGACCTCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fmn1 ( XM_012968079.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823533.1 Xenopus tropicalis formin 1 (fmn1), transcript variant X2, mRNA.

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CloneID	OXa32277
Clone ID Related Accession (Same CDS sequence)	XM_012968079.2
Accession Version	XM_012968079.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	formin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGA AAGAGACTGC TACAAATATG CCAGAGGCGG CAGCTTCTGC TTCAGTAGGG 
AGACCTGATT CATCAGGTCT GTCTATACTG AGTAAATTTT CCATAAAGTC ATTTTTTGGA 
ATCACAACAA GGTTAGACAG CCAGAAAAGC AATGAAGGGA ATGCTGTTTT AAAATCTTTT 
AAATTACTTG GAAATAAATC AGAGTTACAG ACAGACCCTG CGAGCAGTGC ACAGAATTCA 
AGCTGTGAGA GGTCAGTACC ACATTCTGAC ATAACAGGGT GGGAGGCAAA GGACCCTGAA 
GAGCCAAAGG CAACCACTGA TAAACATGTA CAAATTGGAC AGGCGGAAGG CTCAGATCTG 
GATTTGCAAC AGCACAGTTT AAAGGATCTA AGTTGTGAGA ATGAGGGGGA TTCACAAGTC 
ACAGTAGGAC AGCCTGCAGT AGGTGACATT GAAAAGGACA AAGAAAACGC TGAGAAGGAT 
AACCTGGGAG GTGCAGATGT ACTGTTTTTA GATGAGCAAA AGCATGAAAA AAAGGGAGTG 
TGCACAGAAA GTCTGACCGT TTTAGGCACT TGTAAGGATG AAAAAGCTGA TACACATTCC 
AGTGCAGAGC ACGCAGCCTG TGATGTTCCC AGCACAGGAG TTAATGACAA GGATGATTTG 
CTTGTACGTG GTATTCTTGT ACAGACAACA AGTGATACAG ACTCTGACGG TGAGGATACT 
GATCAAGTCA CAGACCAAAA GAGTGCTCCC ATGTCCAAGA ATTTATCGGC ATCTCATGTG 
TCAGTAAACA ATAGTCCCAA ACAGGAGCCA GAAGCAACAG GTCCAGGAAA GACAGGGTAC 
ATGGAGGGAG AAGACAAAGA ACAAGTGAAT ATAAACAGCT CAGGCTCTTT GGGGGCCCAT 
ATGGATGCAC AACAAGGGAT TACACAGGAG GCAGAAATTA ACAGTCCTGA AGACAATTCC 
GTTCCAGAAT CTGTAACAGC TGCATCCCAT TCTGAGCCAC AGAATGAGAA GACCTATGTG 
CTTCCAGCAA TTTTTAGTGG TCTGCGTGTA CACAAGAAAG GAGCACCGGT GGATGATAGG 
GAGATGGTTA CTGTAAAACC AGGTGACAGT GATTTGGCAT TACTTAAATT GTCCCAGACA 
GCTCAAAAAT CCAAAGTCTC CATTGAAACA ATAAGAAAGC GAGAGCAGAA GAAGCCAACA 
GAAACCAGAC CAAGTTCAAA GTTCATGGAA CAACTTTCCC AGCTACTGAG CTTTGATAAC 
CCCAAGCAAG ATGAAACACA GGGGGGATCT ACAGAAGAAC AAAGTATTTC AAATGGCACT 
GAGCAGGACC TCCAGGCTGT TAATGAGGAC AAATTGGAAA CTAGCTCACA AGAGACTGCA 
ATGGATGCTT TCAAATCCTT TTTCATGATT GCTCCCAAAA AGACAATAAC ACAGGATTCC 
CATGACCTGG AGGCTGTCAA GCGCAAGCAA AAGAGTGAGA AGGATACACT GAAGGCTATC 
TTTGAGAAGC CTAGACCCAC AGACCTTGAC CTGGCATCTG ACGAAAAGAA AAATACCGAC 
CTCAGTCCTT CTGATGGAGA AGATCGAACC CCAGGAAGAC TCCAAGCAGT GTGGCCACCC 
CCAAAGCCCA AAGATGAAGA AGAAAAAGTG GGTCTGAAAT ACACAGAAGC AGAGTATCAG 
GCTGCTATCC TGCATTTGAA GAGGGAACAC AAAGATGAGC TAGAGACACT GAAGTCCCAA 
TCTGAAGTGG CGATCTTCAA TGTGCGAGGG GAACAAGCAG TTCAGACAGC AAAATTAGAG 
GAGGAAATAC AGAAACTGCA AGCGGACCTA CAGAATAAAC TCAGTCACCA GAATGGGGAG 
AAAAGGGATG TTTGTGTTTC CACCGAAGAT GAGAATTCTC CAAAATCTTT TCGAAATGTA 
TGCATCCAAA CTGACCGTGA GACTTTTTTG AAACCCAATG AGGAGGACAA ATCTCAAAAA 
AATGACACTG TTTTACCAAA GAAATTAAAT ATTGCATCAA TCAGTCAAAA CCTCGCTAGT 
CCCATAAGTC CCAGCGATAA TTCACAGCAC AATTTCCCTT TAGCTTCCTT GTCAGTTACT 
ACACCACCTC CCCACCCCCT CTCTACATCT TCACAAACAG CCTCTTCTCC AACACCCTTA 
GATTGCACTT CTATTCCTCC ACCTCCATTA CTTCCAGGTT TCCCTTCTGT TCCACCACCT 
CCACCTCTTC CTGGTTCTAG CTCTGTTCCA CCTCCTCCAC CTCTTCCAGG TATCAGTTCT 
GCCCCACCAC CTCCCCCTCT TCCAGGTTTT AGCTCTGTTC CACCTCCTCC TCCTCTTCCA 
GATTTAAGTT CTGTTCCACC CCCTCCCCCT TTTCCAGGTG GGGGTCCTCC TCCACCACCT 
CCTCCTTTTC CTGGGTATGG AAGCTCAGCA GTTCCTCCAC CACTACCTCT ACCACTCCCC 
GGGCTTTTTT TTGGGCTGTC AAAACCTCGA AAAGCCCCTG TAGAGCCTGG CTGCCCAATG 
AAGCCGCTGT ATTGGACAAG GATTCAACTA AAAAATGGCA GCTCCAGCAA TACGTTATGG 
GAGAACCTAA AGGAGCCCAA CATTGCAGAC ACTAAAGAAT TTGAGGACTT ATTTGCAAAA 
GCCACTGTTC AACAGAAGAA GAAACCACTG TCAGACTCTT ATGAGAAGAG AGCAAAAGCT 
AAGCAGGTTA TAAAGCTACT GGATGGGAAG AGGTCTCAGG CTGTGGGCAT TTTAATATCC 
AGTTTACATC TGGACATGAA GGATATTCAA CAAGCAATTC TTAATGTAGA TAATTCAGTT 
GTGGACATAG AAACTCTTGA AGCCTTGTAT GAAAACAGGG CTCAGAAGGA GGAGATGGAA 
ATCATCAAAA AACACTATCA GACATCTAAA GCTGAGGATG TAAAACTGCT GGACAAACCT 
GAACAGTTTC TTTACGAGCT GTCTCAGATT CCCAACTTTG TTGAGCGATC TCAGTGTATT 
ATATTCCAGT CTGTCTTTTT AGAGGGAATA AGCTCAGTGA GGCGGAAGGT GGATATCATC 
AGTCGGGCCT GCGATTGCCT CTTGGAGAGG GTTTCTGTGA GAGATATAAT AGGTCTGATT 
CTTGCATTCG GAAACTACAT GAATGGAGGC AACCGTACTC GGGGTCAGGC AGACGGATTT 
GGGCTGGAAA TCCTTCCCAA ACTAAAGGAT GTTAAAAGCA GGGATAATAA AGCCAGCCTG 
GTGGATTATG TTGTACGTTA TTATCTACGA TATTTTGACC AGGATGCTGG AACAGACAAA 
AGTGTTTTTC CACTGCCAGA ATCCCAGGAC CTTTTACTGG CTTCGCAGGT GAAATTTGAA 
GACCTGGACA AAGACTTACG GAAACTTAAA AAGGACATTG AAGTGTGTGA GAAACAGTTG 
AAAGCAGTGG TAAAAGACTC GCCTAAAGAG AATATCCAGC CATTTAAGGA CAAGATGTCA 
TCTTTTTTAA ACAAAGCCAA AGAAGAATAT AAGACAGAAG AAAGTAAACT GACCAGATCA 
CAAAACAGGT TTGAAGAAAC AATTGGATAT TTTGGATGTA AGCCAAAGGG CAATGATAAA 
GAGATCACCC CCAACTCCTT CTTTGTCTTG TGGTACGAAT TCTGCGGTGA CTTCAAGAGT 
GTGTGGAAAA GAGAGAGCAA AGTCCTCTCC ACGGAAAGGC TTCGCCAGGC TCAGGAGTCC 
GTAAATAAAC TGACAGCAGA AAAGAAAGTT GAAACCAGGA AGATTAACCC TGCAGCAAGT 
CTGAAAGAAA AGCTTCGTCA GAAAGAAGCA AGTGTCAGCA CCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823533.1
CDS	745..4572
Translation	MELKETATNMPEAAASASVGRPDSSGLSILSKFSIKSFFGITTRLDSQKSNEGNAVLKSFKLLGNKSELQTDPASSAQNSSCERSVPHSDITGWEAKDPEEPKATTDKHVQIGQAEGSDLDLQQHSLKDLSCENEGDSQVTVGQPAVGDIEKDKENAEKDNLGGADVLFLDEQKHEKKGVCTESLTVLGTCKDEKADTHSSAEHAACDVPSTGVNDKDDLLVRGILVQTTSDTDSDGEDTDQVTDQKSAPMSKNLSASHVSVNNSPKQEPEATGPGKTGYMEGEDKEQVNINSSGSLGAHMDAQQGITQEAEINSPEDNSVPESVTAASHSEPQNEKTYVLPAIFSGLRVHKKGAPVDDREMVTVKPGDSDLALLKLSQTAQKSKVSIETIRKREQKKPTETRPSSKFMEQLSQLLSFDNPKQDETQGGSTEEQSISNGTEQDLQAVNEDKLETSSQETAMDAFKSFFMIAPKKTITQDSHDLEAVKRKQKSEKDTLKAIFEKPRPTDLDLASDEKKNTDLSPSDGEDRTPGRLQAVWPPPKPKDEEEKVGLKYTEAEYQAAILHLKREHKDELETLKSQSEVAIFNVRGEQAVQTAKLEEEIQKLQADLQNKLSHQNGEKRDVCVSTEDENSPKSFRNVCIQTDRETFLKPNEEDKSQKNDTVLPKKLNIASISQNLASPISPSDNSQHNFPLASLSVTTPPPHPLSTSSQTASSPTPLDCTSIPPPPLLPGFPSVPPPPPLPGSSSVPPPPPLPGISSAPPPPPLPGFSSVPPPPPLPDLSSVPPPPPFPGGGPPPPPPPFPGYGSSAVPPPLPLPLPGLFFGLSKPRKAPVEPGCPMKPLYWTRIQLKNGSSSNTLWENLKEPNIADTKEFEDLFAKATVQQKKKPLSDSYEKRAKAKQVIKLLDGKRSQAVGILISSLHLDMKDIQQAILNVDNSVVDIETLEALYENRAQKEEMEIIKKHYQTSKAEDVKLLDKPEQFLYELSQIPNFVERSQCIIFQSVFLEGISSVRRKVDIISRACDCLLERVSVRDIIGLILAFGNYMNGGNRTRGQADGFGLEILPKLKDVKSRDNKASLVDYVVRYYLRYFDQDAGTDKSVFPLPESQDLLLASQVKFEDLDKDLRKLKKDIEVCEKQLKAVVKDSPKENIQPFKDKMSSFLNKAKEEYKTEESKLTRSQNRFEETIGYFGCKPKGNDKEITPNSFFVLWYEFCGDFKSVWKRESKVLSTERLRQAQESVNKLTAEKKVETRKINPAASLKEKLRQKEASVSTN

Target ORF information:

RefSeq Version	XM_012968079.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis formin 1 (fmn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968079.2

ORF Insert Sequence:

ATGGAGCTGA AAGAGACTGC TACAAATATG CCAGAGGCGG CAGCTTCTGC TTCAGTAGGG 
AGACCTGATT CATCAGGTCT GTCTATACTG AGTAAATTTT CCATAAAGTC ATTTTTTGGA 
ATCACAACAA GGTTAGACAG CCAGAAAAGC AATGAAGGGA ATGCTGTTTT AAAATCTTTT 
AAATTACTTG GAAATAAATC AGAGTTACAG ACAGACCCTG CGAGCAGTGC ACAGAATTCA 
AGCTGTGAGA GGTCAGTACC ACATTCTGAC ATAACAGGGT GGGAGGCAAA GGACCCTGAA 
GAGCCAAAGG CAACCACTGA TAAACATGTA CAAATTGGAC AGGCGGAAGG CTCAGATCTG 
GATTTGCAAC AGCACAGTTT AAAGGATCTA AGTTGTGAGA ATGAGGGGGA TTCACAAGTC 
ACAGTAGGAC AGCCTGCAGT AGGTGACATT GAAAAGGACA AAGAAAACGC TGAGAAGGAT 
AACCTGGGAG GTGCAGATGT ACTGTTTTTA GATGAGCAAA AGCATGAAAA AAAGGGAGTG 
TGCACAGAAA GTCTGACCGT TTTAGGCACT TGTAAGGATG AAAAAGCTGA TACACATTCC 
AGTGCAGAGC ACGCAGCCTG TGATGTTCCC AGCACAGGAG TTAATGACAA GGATGATTTG 
CTTGTACGTG GTATTCTTGT ACAGACAACA AGTGATACAG ACTCTGACGG TGAGGATACT 
GATCAAGTCA CAGACCAAAA GAGTGCTCCC ATGTCCAAGA ATTTATCGGC ATCTCATGTG 
TCAGTAAACA ATAGTCCCAA ACAGGAGCCA GAAGCAACAG GTCCAGGAAA GACAGGGTAC 
ATGGAGGGAG AAGACAAAGA ACAAGTGAAT ATAAACAGCT CAGGCTCTTT GGGGGCCCAT 
ATGGATGCAC AACAAGGGAT TACACAGGAG GCAGAAATTA ACAGTCCTGA AGACAATTCC 
GTTCCAGAAT CTGTAACAGC TGCATCCCAT TCTGAGCCAC AGAATGAGAA GACCTATGTG 
CTTCCAGCAA TTTTTAGTGG TCTGCGTGTA CACAAGAAAG GAGCACCGGT GGATGATAGG 
GAGATGGTTA CTGTAAAACC AGGTGACAGT GATTTGGCAT TACTTAAATT GTCCCAGACA 
GCTCAAAAAT CCAAAGTCTC CATTGAAACA ATAAGAAAGC GAGAGCAGAA GAAGCCAACA 
GAAACCAGAC CAAGTTCAAA GTTCATGGAA CAACTTTCCC AGCTACTGAG CTTTGATAAC 
CCCAAGCAAG ATGAAACACA GGGGGGATCT ACAGAAGAAC AAAGTATTTC AAATGGCACT 
GAGCAGGACC TCCAGGCTGT TAATGAGGAC AAATTGGAAA CTAGCTCACA AGAGACTGCA 
ATGGATGCTT TCAAATCCTT TTTCATGATT GCTCCCAAAA AGACAATAAC ACAGGATTCC 
CATGACCTGG AGGCTGTCAA GCGCAAGCAA AAGAGTGAGA AGGATACACT GAAGGCTATC 
TTTGAGAAGC CTAGACCCAC AGACCTTGAC CTGGCATCTG ACGAAAAGAA AAATACCGAC 
CTCAGTCCTT CTGATGGAGA AGATCGAACC CCAGGAAGAC TCCAAGCAGT GTGGCCACCC 
CCAAAGCCCA AAGATGAAGA AGAAAAAGTG GGTCTGAAAT ACACAGAAGC AGAGTATCAG 
GCTGCTATCC TGCATTTGAA GAGGGAACAC AAAGATGAGC TAGAGACACT GAAGTCCCAA 
TCTGAAGTGG CGATCTTCAA TGTGCGAGGG GAACAAGCAG TTCAGACAGC AAAATTAGAG 
GAGGAAATAC AGAAACTGCA AGCGGACCTA CAGAATAAAC TCAGTCACCA GAATGGGGAG 
AAAAGGGATG TTTGTGTTTC CACCGAAGAT GAGAATTCTC CAAAATCTTT TCGAAATGTA 
TGCATCCAAA CTGACCGTGA GACTTTTTTG AAACCCAATG AGGAGGACAA ATCTCAAAAA 
AATGACACTG TTTTACCAAA GAAATTAAAT ATTGCATCAA TCAGTCAAAA CCTCGCTAGT 
CCCATAAGTC CCAGCGATAA TTCACAGCAC AATTTCCCTT TAGCTTCCTT GTCAGTTACT 
ACACCACCTC CCCACCCCCT CTCTACATCT TCACAAACAG CCTCTTCTCC AACACCCTTA 
GATTGCACTT CTATTCCTCC ACCTCCATTA CTTCCAGGTT TCCCTTCTGT TCCACCACCT 
CCACCTCTTC CTGGTTCTAG CTCTGTTCCA CCTCCTCCAC CTCTTCCAGG TATCAGTTCT 
GCCCCACCAC CTCCCCCTCT TCCAGGTTTT AGCTCTGTTC CACCTCCTCC TCCTCTTCCA 
GATTTAAGTT CTGTTCCACC CCCTCCCCCT TTTCCAGGTG GGGGTCCTCC TCCACCACCT 
CCTCCTTTTC CTGGGTATGG AAGCTCAGCA GTTCCTCCAC CACTACCTCT ACCACTCCCC 
GGGCTTTTTT TTGGGCTGTC AAAACCTCGA AAAGCCCCTG TAGAGCCTGG CTGCCCAATG 
AAGCCGCTGT ATTGGACAAG GATTCAACTA AAAAATGGCA GCTCCAGCAA TACGTTATGG 
GAGAACCTAA AGGAGCCCAA CATTGCAGAC ACTAAAGAAT TTGAGGACTT ATTTGCAAAA 
GCCACTGTTC AACAGAAGAA GAAACCACTG TCAGACTCTT ATGAGAAGAG AGCAAAAGCT 
AAGCAGGTTA TAAAGCTACT GGATGGGAAG AGGTCTCAGG CTGTGGGCAT TTTAATATCC 
AGTTTACATC TGGACATGAA GGATATTCAA CAAGCAATTC TTAATGTAGA TAATTCAGTT 
GTGGACATAG AAACTCTTGA AGCCTTGTAT GAAAACAGGG CTCAGAAGGA GGAGATGGAA 
ATCATCAAAA AACACTATCA GACATCTAAA GCTGAGGATG TAAAACTGCT GGACAAACCT 
GAACAGTTTC TTTACGAGCT GTCTCAGATT CCCAACTTTG TTGAGCGATC TCAGTGTATT 
ATATTCCAGT CTGTCTTTTT AGAGGGAATA AGCTCAGTGA GGCGGAAGGT GGATATCATC 
AGTCGGGCCT GCGATTGCCT CTTGGAGAGG GTTTCTGTGA GAGATATAAT AGGTCTGATT 
CTTGCATTCG GAAACTACAT GAATGGAGGC AACCGTACTC GGGGTCAGGC AGACGGATTT 
GGGCTGGAAA TCCTTCCCAA ACTAAAGGAT GTTAAAAGCA GGGATAATAA AGCCAGCCTG 
GTGGATTATG TTGTACGTTA TTATCTACGA TATTTTGACC AGGATGCTGG AACAGACAAA 
AGTGTTTTTC CACTGCCAGA ATCCCAGGAC CTTTTACTGG CTTCGCAGGT GAAATTTGAA 
GACCTGGACA AAGACTTACG GAAACTTAAA AAGGACATTG AAGTGTGTGA GAAACAGTTG 
AAAGCAGTGG TAAAAGACTC GCCTAAAGAG AATATCCAGC CATTTAAGGA CAAGATGTCA 
TCTTTTTTAA ACAAAGCCAA AGAAGAATAT AAGACAGAAG AAAGTAAACT GACCAGATCA 
CAAAACAGGT TTGAAGAAAC AATTGGATAT TTTGGATGTA AGCCAAAGGG CAATGATAAA 
GAGATCACCC CCAACTCCTT CTTTGTCTTG TGGTACGAAT TCTGCGGTGA CTTCAAGAGT 
GTGTGGAAAA GAGAGAGCAA AGTCCTCTCC ACGGAAAGGC TTCGCCAGGC TCAGGAGTCC 
GTAAATAAAC TGACAGCAGA AAAGAAAGTT GAAACCAGGA AGATTAACCC TGCAGCAAGT 
CTGAAAGAAA AGCTTCGTCA GAAAGAAGCA AGTGTCAGCA CCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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fmn1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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