polr3b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	polr3b
Entrez Gene ID	100158643
Full Name	polymerase (RNA) III (DNA directed) polypeptide B
General protein information	Preferred Names polymerase (RNA) III (DNA directed) polypeptide B Names DNA-directed RNA polymerase III subunit RPC2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128049.1	NP_001121521.1	DNA-directed RNA polymerase III subunit RPC2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RpIII128	NP_523706.1
Kluyveromyces lactis	KLLA0F01078g	XP_455128.1
Schizosaccharomyces pombe (fission yeast)	rpc2	NP_593690.1
Mus musculus (house mouse)	Polr3b	NP_081699.1
Rattus norvegicus (Norway rat)	Polr3b	NP_001178807.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008151	XP_317312.4
Neurospora crassa	NCU01772	XP_957195.1
Oryza sativa (rice)	Os03g0403100	NP_001050323.1
Homo sapiens (human)	POLR3B	NP_060552.4
Gallus gallus (chicken)	POLR3B	XP_416307.3
Danio rerio (zebrafish)	polr3b	XP_690811.2
	POLR3B	XP_001099515.1
Magnaporthe oryzae (rice blast fungus)	MGG_06984	XP_003709735.1
Xenopus tropicalis (tropical clawed frog)	polr3b	NP_001121521.1
Pan troglodytes (chimpanzee)	POLR3B	XP_001161567.1
Canis lupus familiaris (dog)	POLR3B	XP_531760.2
Bos taurus (cattle)	POLR3B	NP_001179718.1
Caenorhabditis elegans (roundworm)	F09F7.3	NP_498192.2
Saccharomyces cerevisiae (baker's yeast)	RET1	NP_014850.1
Eremothecium gossypii	AGOS_ADL275C	NP_983821.2
Arabidopsis thaliana (thale cress)	NRPC2	NP_199327.4

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following polr3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the polr3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa20751	NM_001128049.1 Latest version!	Xenopus tropicalis polymerase (RNA) III (DNA directed) polypeptide B (polr3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

polr3b ( NM_001128049.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001121521.1 Xenopus tropicalis polymerase (RNA) III (DNA directed) polypeptide B (polr3b), mRNA.

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CloneID	OXa20751
Clone ID Related Accession (Same CDS sequence)	NM_001128049.1
Accession Version	NM_001128049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA-directed RNA polymerase III subunit RPC2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166377.1. ##Evidence-Data-START## Transcript exon combination :: BC166377.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGTGT TGGATGTGGA GTTTGGTCAT CTGAGTCAGG AGGAGCTCAT TGCTCCCGTT 
GATACAGTGC AGGAAAAATG GAAACTGCTT CCAGCCTTCC TAAAGGTGAA GGGCCTTGTA 
AAGCAGCACA TTGACTCCTT CAACTATTTC ATCAATGATG AAATTAAAAA AATAATGAAA 
GCAAATGAAA AAGTGACAAG CGATGCTGAC CCAATGTGGT ACCTAAAGTA TCTTAATATT 
TACGTGGGTC TTCCTGATGT TGAAGAAAGT TTTAATGTGA CTCGGCCTGT ATCTCCTCAT 
GAGTGCCGAC TCAGAGATAT GACCTACTCG GCACCAATTA CAGTTGACAT AGAGTACACT 
AGAGGCAGCC AAAGAATCAT TCGCAATGCG TTGCCTATTG GCAGAATGCC CATCATGCTG 
CGCAGCTCTA ATTGTGTCCT TACTGGAAAG ACCCCAGCTG AGTTTGCAAA GCTAAATGAA 
TGTCCTCTAG ATCCCGGTGG TTACTTCATT GTTAAAGGAG TAGAAAAGGT AATTCTTATT 
CAGGAGCAGC TCTCCAAAAA CAGGATCATT GTGGAAGCAG ATAGGAAAGG AACTGTTGGG 
GCCTCTGTTA CCAGCTCTAC TCATGAGAAG AAAAGCCGAA CTAACTTGGT TGTGAAGCAG 
GGAAGGTTTT ACCTGAAACA CAATACCTTA ACAGAAGACA TCCCAATAGC CATTATTTTT 
AAGGCAATGG GCGTCGAGAG TGACCAGGAG ATAGTGCAGA TGATTGGCAC AGAGGAACAC 
GTGATCGCTG CCTTCGCACC TAGCTTGGAG GAGTGTCAGA AGGCACAGAT CTTCACACAG 
ATGCAGGCTT TAAAGTTCAT TGGCAATAAA GTGCGCAGGC AGCGCATGTG GGGAAGCAGC 
GGCCCTAAAA AATCAAAAAT TGATGAAGCC CGTGAGCTGT TGGCATCCAC CATCCTGACT 
CATGTCCCTG TAAAAGAATT CCATTTCCGT ACAAAGTGCA TTTACATCGC TGTAATGGTC 
CGGAGGGTGA TTCTGGCCCA AGGGGCAAAC AAAGTAGACG ATAGAGATTA CTACGGAAAT 
AAAAGATTGG AACTGGCAGG ACAGCTGCTT TCGTTGCTTT TTGAAGATTT ATTCAAGAAG 
TTTAACTCTG AGATGAAAAA GATAGCAGAC CAAGTCATCC CCAAGCAGAG GGCAGCACAG 
TTTGATGTGG TTAAACACAT GCGCCAAGAC CAGATAACCA ACGGCATGGT AAATGCCATC 
TCATCAGGGA ACTGGTCGCT AAAAAGATTC AAAATGGACC GCCAAGGTGT GACACAAGTC 
CTGTCGCGGT TATCGTACAT AGCCGCCTTG GGCATGATGA CCAGGATTTC TTCTCAGTTT 
GAAAAAACGA GAAAAGTGAG TGGCCCGCGT TCTTTGCAGC CATCCCAGTG GGGTATGCTG 
TGCCCTTCTG ACACTCCTGA AGGAGAGGCT TGTGGCTTGG TGAAGAATTT GGCACTGATG 
ACCCACATCA CTACTGATAT GGAAGATGGC CCTATTATCA AACTGGCAGC TAATCTTGGG 
GTAGAGGATG TGAACTTGCT TTGTGGAGAG GAACTGTCCT ATGCCAATGT CTTTCTGGTC 
TTCCTTAATG GTAACATCCT TGGTGTTATC CGAGACCATC AGAAGCTGGT GAACACCTTT 
AGGTTAATGC GAAGAGCCGG CTACATCAAC GAATTTGTTT CCATTTCCAC CAACATTTCC 
GACCGCTGCG TGTACATCTC CTCTGATGGG GGCAGACTAT GCAGGCCTTA TATTATTGTG 
AAAAACCAGA GGTCTGCAGT GACAAATAAG CACATCCAGG AGCTTTCTCA GGGATACAGG 
AGCTTTGAGG ATTTCCTTCA TGAAAGTCTT GTTGAGTATT TGGATGTCAA TGAAGAAAAT 
GATTGTAACG TAGCTCTGTA TGAACACACT ATAACCAAGC ATACAACACA CTTGGAAATT 
GAGCCTTTCA CGTTACTTGG CGTTTGTGCT GGTCTGATTC CCTACCCGCA CCATAATCAG 
TCCCCAAGAA ACACATACCA GTGTGCTATG GGGAAACAAG CTATGGGGAC CATTGGGTAT 
AACCAGAGGA ACAGGATTGA TACCCTGATG TACCTACTGG CCTATCCACA AAGGCCAATG 
GTTAAAACGA AAACCATTGA GCTTATTGAA TTTGAGAAAC TCCCAGCGGG GCAGAATGCG 
ACGGTCGCTG TTATGAGCTA TAGCGGCTAT GATATTGAGG ATGCCCTTGT ATTAAATAAA 
GCCTCCCTGG ATAGAGGGTT TGGTCGCTGC TTAGTGTACA AGAATGCCAA ATGTACCCTA 
AAACGCTACA GCAACCAGAC GTTTGACAAG GTGATGGGGC CAATGTTGGA TGCAAATACC 
CGAAAGCAAA TCTGGAGGCA TGAAATCTTA GATGCTGATG GGATCTGCTC ACCAGGGGAA 
AAAGTGGAGA ACAAACAAGT TCTTGTCAAC AAGTACATGC CGACTGTCAC ACAGACCCCT 
CTAGAGGGGA GCAGCGTGCC ACAACAGCCG CAGTACAAGG ATGTGCCTAT AACTTACAAA 
GGGCCCACAG ACTCATATAT TGAAAAAGTC ATGATTTCTT CAAATGCAGA AGATGCATTT 
CTCATTAAAA TTCTACTCCG ACAAACCAGG CGGCCAGAAA TTGGGGATAA GTTCAGCAGC 
CGGCATGGAC AAAAAGGTGT TTGCGGTTTG ATTGTCCCTC AAGAGGATAT GCCATTTTGT 
GAAACTGGCA TCTGCCCAGA CATTATCATG AATCCTCACG GCTACCCATC CCGTATGACG 
GTTGGAAAGC TGATCGAACT CTTGGCAGGG AAAGCCGGGG TCCTTGATGG CAGATTCCAC 
TATGGAACAG CCTTTGGAGG CAGTAAAGTG AAGGACGTGT GCGAAGATCT CATTCGATAT 
GGCTACAACT ACTTGGGGAA GGACTATGTA ACATCTGGTA TCACAGGGGA ACCTCTAGAA 
GCCTATATTT ATTTTGGTCC GGTGTATTAT CAGAAGCTAA AGCACATGGT GTTGGATAAG 
ATGCATGCCC GAGCAAGAGG TCCCAGGGCA GTGCTAACAA GGCAGCCCAC GGAAGGTCGA 
TCCCGAGATG GCGGCCTGCG TCTCGGAGAA ATGGAACGTG ACTGCCTGAT TGGCTATGGG 
GCCAGCATGC TGCTATTGGA GAGGCTGATG ATTTCTAGTG ACGCCTTTGA AGTGGACGTC 
TGTGGGAAGT GCGGCCTGCT CGGATACTCC GGCTGGTGCC ATTTCTGCAA GTCGTCTTGT 
CATGTCTCCT CCTTGAGGAT CCCCTACGCC TGCAAGTTGC TCTTCCAAGA GCTCCAGTCA 
ATGAATATTA TCCCCAGACT CAAACTGGCC AAGTATAATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121521.1
CDS	17..3421
Translation	MEVLDVEFGHLSQEELIAPVDTVQEKWKLLPAFLKVKGLVKQHIDSFNYFINDEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGTVGASVTSSTHEKKSRTNLVVKQGRFYLKHNTLTEDIPIAIIFKAMGVESDQEIVQMIGTEEHVIAAFAPSLEECQKAQIFTQMQALKFIGNKVRRQRMWGSSGPKKSKIDEARELLASTILTHVPVKEFHFRTKCIYIAVMVRRVILAQGANKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISSGNWSLKRFKMDRQGVTQVLSRLSYIAALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIIKLAANLGVEDVNLLCGEELSYANVFLVFLNGNILGVIRDHQKLVNTFRLMRRAGYINEFVSISTNISDRCVYISSDGGRLCRPYIIVKNQRSAVTNKHIQELSQGYRSFEDFLHESLVEYLDVNEENDCNVALYEHTITKHTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQRPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYSNQTFDKVMGPMLDANTRKQIWRHEILDADGICSPGEKVENKQVLVNKYMPTVTQTPLEGSSVPQQPQYKDVPITYKGPTDSYIEKVMISSNAEDAFLIKILLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCETGICPDIIMNPHGYPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLIRYGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGKCGLLGYSGWCHFCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLAKYNE

Target ORF information:

RefSeq Version	NM_001128049.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (RNA) III (DNA directed) polypeptide B (polr3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128049.1

ORF Insert Sequence:

ATGGAGGTGT TGGATGTGGA GTTTGGTCAT CTGAGTCAGG AGGAGCTCAT TGCTCCCGTT 
GATACAGTGC AGGAAAAATG GAAACTGCTT CCAGCCTTCC TAAAGGTGAA GGGCCTTGTA 
AAGCAGCACA TTGACTCCTT CAACTATTTC ATCAATGATG AAATTAAAAA AATAATGAAA 
GCAAATGAAA AAGTGACAAG CGATGCTGAC CCAATGTGGT ACCTAAAGTA TCTTAATATT 
TACGTGGGTC TTCCTGATGT TGAAGAAAGT TTTAATGTGA CTCGGCCTGT ATCTCCTCAT 
GAGTGCCGAC TCAGAGATAT GACCTACTCG GCACCAATTA CAGTTGACAT AGAGTACACT 
AGAGGCAGCC AAAGAATCAT TCGCAATGCG TTGCCTATTG GCAGAATGCC CATCATGCTG 
CGCAGCTCTA ATTGTGTCCT TACTGGAAAG ACCCCAGCTG AGTTTGCAAA GCTAAATGAA 
TGTCCTCTAG ATCCCGGTGG TTACTTCATT GTTAAAGGAG TAGAAAAGGT AATTCTTATT 
CAGGAGCAGC TCTCCAAAAA CAGGATCATT GTGGAAGCAG ATAGGAAAGG AACTGTTGGG 
GCCTCTGTTA CCAGCTCTAC TCATGAGAAG AAAAGCCGAA CTAACTTGGT TGTGAAGCAG 
GGAAGGTTTT ACCTGAAACA CAATACCTTA ACAGAAGACA TCCCAATAGC CATTATTTTT 
AAGGCAATGG GCGTCGAGAG TGACCAGGAG ATAGTGCAGA TGATTGGCAC AGAGGAACAC 
GTGATCGCTG CCTTCGCACC TAGCTTGGAG GAGTGTCAGA AGGCACAGAT CTTCACACAG 
ATGCAGGCTT TAAAGTTCAT TGGCAATAAA GTGCGCAGGC AGCGCATGTG GGGAAGCAGC 
GGCCCTAAAA AATCAAAAAT TGATGAAGCC CGTGAGCTGT TGGCATCCAC CATCCTGACT 
CATGTCCCTG TAAAAGAATT CCATTTCCGT ACAAAGTGCA TTTACATCGC TGTAATGGTC 
CGGAGGGTGA TTCTGGCCCA AGGGGCAAAC AAAGTAGACG ATAGAGATTA CTACGGAAAT 
AAAAGATTGG AACTGGCAGG ACAGCTGCTT TCGTTGCTTT TTGAAGATTT ATTCAAGAAG 
TTTAACTCTG AGATGAAAAA GATAGCAGAC CAAGTCATCC CCAAGCAGAG GGCAGCACAG 
TTTGATGTGG TTAAACACAT GCGCCAAGAC CAGATAACCA ACGGCATGGT AAATGCCATC 
TCATCAGGGA ACTGGTCGCT AAAAAGATTC AAAATGGACC GCCAAGGTGT GACACAAGTC 
CTGTCGCGGT TATCGTACAT AGCCGCCTTG GGCATGATGA CCAGGATTTC TTCTCAGTTT 
GAAAAAACGA GAAAAGTGAG TGGCCCGCGT TCTTTGCAGC CATCCCAGTG GGGTATGCTG 
TGCCCTTCTG ACACTCCTGA AGGAGAGGCT TGTGGCTTGG TGAAGAATTT GGCACTGATG 
ACCCACATCA CTACTGATAT GGAAGATGGC CCTATTATCA AACTGGCAGC TAATCTTGGG 
GTAGAGGATG TGAACTTGCT TTGTGGAGAG GAACTGTCCT ATGCCAATGT CTTTCTGGTC 
TTCCTTAATG GTAACATCCT TGGTGTTATC CGAGACCATC AGAAGCTGGT GAACACCTTT 
AGGTTAATGC GAAGAGCCGG CTACATCAAC GAATTTGTTT CCATTTCCAC CAACATTTCC 
GACCGCTGCG TGTACATCTC CTCTGATGGG GGCAGACTAT GCAGGCCTTA TATTATTGTG 
AAAAACCAGA GGTCTGCAGT GACAAATAAG CACATCCAGG AGCTTTCTCA GGGATACAGG 
AGCTTTGAGG ATTTCCTTCA TGAAAGTCTT GTTGAGTATT TGGATGTCAA TGAAGAAAAT 
GATTGTAACG TAGCTCTGTA TGAACACACT ATAACCAAGC ATACAACACA CTTGGAAATT 
GAGCCTTTCA CGTTACTTGG CGTTTGTGCT GGTCTGATTC CCTACCCGCA CCATAATCAG 
TCCCCAAGAA ACACATACCA GTGTGCTATG GGGAAACAAG CTATGGGGAC CATTGGGTAT 
AACCAGAGGA ACAGGATTGA TACCCTGATG TACCTACTGG CCTATCCACA AAGGCCAATG 
GTTAAAACGA AAACCATTGA GCTTATTGAA TTTGAGAAAC TCCCAGCGGG GCAGAATGCG 
ACGGTCGCTG TTATGAGCTA TAGCGGCTAT GATATTGAGG ATGCCCTTGT ATTAAATAAA 
GCCTCCCTGG ATAGAGGGTT TGGTCGCTGC TTAGTGTACA AGAATGCCAA ATGTACCCTA 
AAACGCTACA GCAACCAGAC GTTTGACAAG GTGATGGGGC CAATGTTGGA TGCAAATACC 
CGAAAGCAAA TCTGGAGGCA TGAAATCTTA GATGCTGATG GGATCTGCTC ACCAGGGGAA 
AAAGTGGAGA ACAAACAAGT TCTTGTCAAC AAGTACATGC CGACTGTCAC ACAGACCCCT 
CTAGAGGGGA GCAGCGTGCC ACAACAGCCG CAGTACAAGG ATGTGCCTAT AACTTACAAA 
GGGCCCACAG ACTCATATAT TGAAAAAGTC ATGATTTCTT CAAATGCAGA AGATGCATTT 
CTCATTAAAA TTCTACTCCG ACAAACCAGG CGGCCAGAAA TTGGGGATAA GTTCAGCAGC 
CGGCATGGAC AAAAAGGTGT TTGCGGTTTG ATTGTCCCTC AAGAGGATAT GCCATTTTGT 
GAAACTGGCA TCTGCCCAGA CATTATCATG AATCCTCACG GCTACCCATC CCGTATGACG 
GTTGGAAAGC TGATCGAACT CTTGGCAGGG AAAGCCGGGG TCCTTGATGG CAGATTCCAC 
TATGGAACAG CCTTTGGAGG CAGTAAAGTG AAGGACGTGT GCGAAGATCT CATTCGATAT 
GGCTACAACT ACTTGGGGAA GGACTATGTA ACATCTGGTA TCACAGGGGA ACCTCTAGAA 
GCCTATATTT ATTTTGGTCC GGTGTATTAT CAGAAGCTAA AGCACATGGT GTTGGATAAG 
ATGCATGCCC GAGCAAGAGG TCCCAGGGCA GTGCTAACAA GGCAGCCCAC GGAAGGTCGA 
TCCCGAGATG GCGGCCTGCG TCTCGGAGAA ATGGAACGTG ACTGCCTGAT TGGCTATGGG 
GCCAGCATGC TGCTATTGGA GAGGCTGATG ATTTCTAGTG ACGCCTTTGA AGTGGACGTC 
TGTGGGAAGT GCGGCCTGCT CGGATACTCC GGCTGGTGCC ATTTCTGCAA GTCGTCTTGT 
CATGTCTCCT CCTTGAGGAT CCCCTACGCC TGCAAGTTGC TCTTCCAAGA GCTCCAGTCA 
ATGAATATTA TCCCCAGACT CAAACTGGCC AAGTATAATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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polr3b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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