espl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following espl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the espl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa52740 XM_004911948.4
Latest version!
Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa52740 XM_031897204.1
Latest version!
Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa52740 XM_031897205.1
Latest version!
Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa06113 XM_004911948.3
Latest version!
Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa52740
    Clone ID Related Accession (Same CDS sequence) XM_004911948.4 , XM_031897204.1 , XM_031897205.1
    Accession Version XM_004911948.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product separin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911948.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
    GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT
    CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA
    GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA
    TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG
    TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG
    TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT
    TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA
    CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG
    CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA
    CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA
    GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT
    GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC
    AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA
    TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG
    GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA
    ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT
    TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT
    GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA
    GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC
    AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG
    GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC
    AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC
    TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC
    CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG
    GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT
    CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC
    TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA
    AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG
    GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT
    GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG
    GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT
    TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT
    CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT
    CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA
    AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC
    GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT
    ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC
    AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA
    TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC
    TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT
    CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC
    CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG
    GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG
    GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG
    GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA
    TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT
    GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA
    AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA
    AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG
    AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC
    AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA
    GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG
    TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA
    AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC
    CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA
    AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG
    TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC
    ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA
    GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT
    GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG
    TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA
    CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT
    CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA
    GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC
    CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA
    CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC
    ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC
    CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA
    AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC
    TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA
    CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA
    CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC
    ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG
    ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC
    TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA
    CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA
    GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA
    GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC
    AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT
    CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA
    AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG
    CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA
    GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG
    CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG
    TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG
    GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA
    CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG
    CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG
    GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA
    GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT
    GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA
    GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT
    CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA
    AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG
    CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA
    GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG
    CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT
    CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG
    TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG
    AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA
    CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT
    GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC
    CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA
    GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA
    GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC
    TTACCAGTGG CCCTGCGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912005.2
    CDS116..6496
    Translation

    Target ORF information:

    RefSeq Version XM_004911948.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911948.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
    GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT
    CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA
    GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA
    TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG
    TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG
    TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT
    TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA
    CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG
    CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA
    CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA
    GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT
    GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC
    AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA
    TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG
    GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA
    ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT
    TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT
    GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA
    GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC
    AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG
    GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC
    AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC
    TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC
    CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG
    GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT
    CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC
    TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA
    AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG
    GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT
    GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG
    GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT
    TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT
    CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT
    CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA
    AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC
    GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT
    ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC
    AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA
    TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC
    TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT
    CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC
    CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG
    GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG
    GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG
    GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA
    TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT
    GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA
    AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA
    AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG
    AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC
    AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA
    GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG
    TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA
    AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC
    CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA
    AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG
    TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC
    ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA
    GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT
    GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG
    TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA
    CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT
    CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA
    GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC
    CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA
    CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC
    ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC
    CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA
    AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC
    TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA
    CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA
    CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC
    ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG
    ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC
    TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA
    CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA
    GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA
    GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC
    AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT
    CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA
    AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG
    CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA
    GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG
    CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG
    TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG
    GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA
    CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG
    CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG
    GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA
    GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT
    GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA
    GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT
    CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA
    AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG
    CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA
    GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG
    CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT
    CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG
    TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG
    AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA
    CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT
    GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC
    CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA
    GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA
    GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC
    TTACCAGTGG CCCTGCGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52740
    Clone ID Related Accession (Same CDS sequence) XM_004911948.4 , XM_031897204.1 , XM_031897205.1
    Accession Version XM_031897204.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product separin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
    GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT
    CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA
    GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA
    TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG
    TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG
    TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT
    TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA
    CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG
    CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA
    CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA
    GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT
    GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC
    AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA
    TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG
    GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA
    ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT
    TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT
    GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA
    GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC
    AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG
    GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC
    AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC
    TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC
    CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG
    GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT
    CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC
    TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA
    AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG
    GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT
    GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG
    GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT
    TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT
    CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT
    CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA
    AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC
    GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT
    ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC
    AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA
    TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC
    TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT
    CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC
    CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG
    GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG
    GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG
    GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA
    TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT
    GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA
    AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA
    AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG
    AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC
    AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA
    GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG
    TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA
    AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC
    CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA
    AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG
    TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC
    ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA
    GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT
    GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG
    TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA
    CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT
    CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA
    GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC
    CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA
    CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC
    ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC
    CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA
    AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC
    TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA
    CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA
    CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC
    ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG
    ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC
    TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA
    CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA
    GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA
    GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC
    AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT
    CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA
    AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG
    CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA
    GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG
    CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG
    TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG
    GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA
    CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG
    CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG
    GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA
    GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT
    GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA
    GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT
    CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA
    AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG
    CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA
    GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG
    CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT
    CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG
    TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG
    AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA
    CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT
    GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC
    CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA
    GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA
    GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC
    TTACCAGTGG CCCTGCGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753064.1
    CDS103..6483
    Translation

    Target ORF information:

    RefSeq Version XM_031897204.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897204.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
    GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT
    CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA
    GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA
    TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG
    TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG
    TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT
    TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA
    CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG
    CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA
    CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA
    GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT
    GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC
    AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA
    TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG
    GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA
    ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT
    TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT
    GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA
    GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC
    AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG
    GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC
    AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC
    TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC
    CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG
    GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT
    CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC
    TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA
    AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG
    GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT
    GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG
    GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT
    TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT
    CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT
    CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA
    AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC
    GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT
    ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC
    AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA
    TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC
    TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT
    CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC
    CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG
    GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG
    GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG
    GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA
    TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT
    GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA
    AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA
    AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG
    AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC
    AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA
    GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG
    TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA
    AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC
    CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA
    AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG
    TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC
    ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA
    GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT
    GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG
    TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA
    CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT
    CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA
    GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC
    CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA
    CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC
    ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC
    CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA
    AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC
    TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA
    CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA
    CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC
    ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG
    ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC
    TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA
    CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA
    GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA
    GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC
    AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT
    CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA
    AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG
    CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA
    GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG
    CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG
    TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG
    GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA
    CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG
    CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG
    GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA
    GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT
    GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA
    GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT
    CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA
    AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG
    CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA
    GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG
    CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT
    CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG
    TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG
    AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA
    CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT
    GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC
    CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA
    GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA
    GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC
    TTACCAGTGG CCCTGCGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52740
    Clone ID Related Accession (Same CDS sequence) XM_004911948.4 , XM_031897204.1 , XM_031897205.1
    Accession Version XM_031897205.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product separin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
    GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT
    CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA
    GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA
    TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG
    TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG
    TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT
    TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA
    CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG
    CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA
    CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA
    GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT
    GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC
    AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA
    TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG
    GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA
    ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT
    TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT
    GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA
    GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC
    AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG
    GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC
    AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC
    TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC
    CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG
    GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT
    CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC
    TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA
    AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG
    GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT
    GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG
    GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT
    TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT
    CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT
    CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA
    AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC
    GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT
    ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC
    AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA
    TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC
    TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT
    CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC
    CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG
    GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG
    GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG
    GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA
    TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT
    GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA
    AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA
    AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG
    AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC
    AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA
    GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG
    TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA
    AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC
    CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA
    AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG
    TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC
    ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA
    GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT
    GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG
    TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA
    CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT
    CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA
    GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC
    CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA
    CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC
    ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC
    CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA
    AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC
    TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA
    CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA
    CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC
    ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG
    ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC
    TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA
    CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA
    GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA
    GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC
    AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT
    CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA
    AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG
    CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA
    GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG
    CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG
    TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG
    GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA
    CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG
    CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG
    GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA
    GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT
    GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA
    GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT
    CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA
    AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG
    CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA
    GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG
    CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT
    CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG
    TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG
    AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA
    CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT
    GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC
    CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA
    GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA
    GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC
    TTACCAGTGG CCCTGCGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753065.1
    CDS31..6411
    Translation

    Target ORF information:

    RefSeq Version XM_031897205.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897205.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
    GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT
    CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA
    GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA
    TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG
    TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG
    TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT
    TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA
    CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG
    CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA
    CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA
    GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT
    GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC
    AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA
    TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG
    GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA
    ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT
    TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT
    GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA
    GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC
    AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG
    GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC
    AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC
    TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC
    CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG
    GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT
    CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC
    TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA
    AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG
    GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT
    GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG
    GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT
    TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT
    CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT
    CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA
    AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC
    GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT
    ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC
    AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA
    TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC
    TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT
    CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC
    CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG
    GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG
    GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG
    GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA
    TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT
    GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA
    AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA
    AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG
    AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC
    AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA
    GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG
    TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA
    AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC
    CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA
    AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG
    TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC
    ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA
    GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT
    GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG
    TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA
    CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT
    CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA
    GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC
    CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA
    CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC
    ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC
    CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA
    AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC
    TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA
    CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA
    CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC
    ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG
    ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC
    TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA
    CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA
    GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA
    GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC
    AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT
    CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA
    AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG
    CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA
    GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG
    CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG
    TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG
    GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA
    CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG
    CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG
    GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA
    GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT
    GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA
    GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT
    CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA
    AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG
    CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA
    GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG
    CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT
    CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG
    TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG
    AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA
    CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT
    GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC
    CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA
    GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA
    GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC
    TTACCAGTGG CCCTGCGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06113
    Clone ID Related Accession (Same CDS sequence) XM_004911948.3
    Accession Version XM_004911948.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product separin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911948.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCATTT 
    GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT
    CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA
    GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA
    TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG
    TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG
    TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT
    TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA
    CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG
    CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA
    CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA
    GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT
    GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC
    AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA
    TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG
    GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA
    ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT
    TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT
    GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA
    GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC
    AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG
    GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC
    AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC
    TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC
    CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG
    GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT
    CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC
    TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA
    AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG
    GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT
    GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG
    GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT
    TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT
    CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT
    CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA
    AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC
    GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT
    ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC
    AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA
    TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC
    TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GGTTGGGGCT
    CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC
    CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG
    GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG
    GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG
    GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA
    TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT
    GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA
    AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA
    AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG
    AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC
    AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA
    GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG
    TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA
    AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC
    CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA
    AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG
    TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC
    ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA
    GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT
    GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG
    TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA
    CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT
    CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA
    GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC
    CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA
    CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC
    ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC
    CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA
    AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC
    TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA
    CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA
    CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC
    ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG
    ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC
    TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA
    CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA
    GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA
    GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC
    AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT
    CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA
    AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG
    CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA
    GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG
    CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG
    TTCCAAGAGC AACTACACCA GATCCCTGCA AATACAACAA TGTGTATATT GACCTTGGTG
    GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA
    CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG
    CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG
    GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA
    GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT
    GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA
    GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT
    CAGTCCTTCA TCCACGGATT TGGATTTTTA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA
    AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG
    CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA
    GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG
    CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT
    CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG
    TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG
    AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA
    CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT
    GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC
    CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA
    GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA
    GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC
    TTACCAGTGG CCCTGCGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912005.1
    CDS117..6497
    Translation

    Target ORF information:

    RefSeq Version XM_004911948.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911948.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCATTT 
    GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT
    CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA
    GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA
    TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG
    TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG
    TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT
    TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA
    CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG
    CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA
    CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA
    GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT
    GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC
    AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA
    TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG
    GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA
    ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT
    TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT
    GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA
    GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC
    AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG
    GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC
    AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC
    TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC
    CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG
    GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT
    CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC
    TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA
    AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG
    GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT
    GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG
    GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT
    TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT
    CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT
    CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA
    AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC
    GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT
    ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC
    AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA
    TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC
    TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GGTTGGGGCT
    CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC
    CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG
    GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG
    GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG
    GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA
    TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT
    GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA
    AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA
    AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG
    AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC
    AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA
    GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG
    TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA
    AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC
    CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA
    AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG
    TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC
    ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA
    GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT
    GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG
    TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA
    CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT
    CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA
    GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC
    CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA
    CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC
    ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC
    CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA
    AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC
    TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA
    CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA
    CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC
    ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG
    ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC
    TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA
    CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA
    GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA
    GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC
    AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT
    CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA
    AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG
    CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA
    GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG
    CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG
    TTCCAAGAGC AACTACACCA GATCCCTGCA AATACAACAA TGTGTATATT GACCTTGGTG
    GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA
    CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG
    CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG
    GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA
    GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT
    GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA
    GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT
    CAGTCCTTCA TCCACGGATT TGGATTTTTA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA
    AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG
    CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA
    GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG
    CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT
    CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG
    TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG
    AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA
    CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT
    GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC
    CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA
    GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA
    GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC
    TTACCAGTGG CCCTGCGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.