polr1a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	polr1a
Entrez Gene ID	100158635
Full Name	polymerase (RNA) I polypeptide A
General protein information	Preferred Names polymerase (RNA) I polypeptide A Names DNA-directed RNA polymerase I subunit RPA1 polymerase (RNA) I polypeptide A, 194kDa
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031895296.1	XP_031751156.1	DNA-directed RNA polymerase I subunit RPA1
XM_012955748.2	XP_012811202.1	DNA-directed RNA polymerase I subunit RPA1

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR1A	NP_056240.2
Pan troglodytes (chimpanzee)	POLR1A	XP_001135380.2
Caenorhabditis elegans (roundworm)	Y48E1A.1	NP_001022450.1
Eremothecium gossypii	AGOS_ADR374C	NP_984470.2
Schizosaccharomyces pombe (fission yeast)	nuc1	NP_596300.1
Saccharomyces cerevisiae (baker's yeast)	RPA190	NP_014986.3
Magnaporthe oryzae (rice blast fungus)	MGG_01158	XP_003717763.1
	POLR1A	XP_002808090.1
Gallus gallus (chicken)	POLR1A	XP_420851.3
Danio rerio (zebrafish)	polr1a	XP_001922839.2
Drosophila melanogaster (fruit fly)	RpI1	NP_523743.1
Xenopus tropicalis (tropical clawed frog)	polr1a	XP_002937328.2
Bos taurus (cattle)	POLR1A	NP_001179805.1
Rattus norvegicus (Norway rat)	Polr1a	NP_113960.1
Neurospora crassa	NCU01638	XP_956006.2
Arabidopsis thaliana (thale cress)	NRPA1	NP_191325.1
Canis lupus familiaris (dog)	POLR1A	XP_532976.3
Mus musculus (house mouse)	Polr1a	NP_033114.3
Kluyveromyces lactis	KLLA0F23243g	XP_456115.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following polr1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the polr1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa49594	XM_031895296.1 Latest version!	Xenopus tropicalis polymerase (RNA) I polypeptide A (polr1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa32236	XM_012955748.2 Latest version!	Xenopus tropicalis polymerase (RNA) I polypeptide A (polr1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

polr1a ( XM_031895296.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751156.1 Xenopus tropicalis polymerase (RNA) I polypeptide A (polr1a), mRNA.

Price & Availability↑

CloneID	OXa49594
Clone ID Related Accession (Same CDS sequence)	XM_031895296.1
Accession Version	XM_031895296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGTAG GAAGTCTGCG GGCTGTACCA CTGGCTGAGG GGGGGGGGGG CAGGTCGCAC 
GGCGCTAGAG CAGGACTCCT CCACTTGTCA GGCAGTTGGT ACGTTCCACT TCCGGGTCCC 
CACGTGTGGC CGGCAGAAGT CCGGGAGGCG GAGAGGTTTG AGGGAACCGG GCAAGCGGCG 
CAGTGTGGCC TCCCCTGCCG GGCAATGCTC ATCTCTAAGG ACGTGCCATG GCGGCGCCTG 
CAAGGCGTCT CTTTCGGCTG TTACTCAGCG GAGGAGATCA GGAAGCTGAG TGTGAAAGCC 
CTGACCAACC CTCACTATAT GGACAACCTG GGCAATCCGA TGATGAACGG CCTGTATGAT 
CTGGCCCTGG GTCCCGCTGA CGGCAAAGAA GTGTGCTCCA CCTGCGCTCA GGACTTCAAT 
AACTGCCCCG GGCACCTGGG GCACATCGAG CTGCCCCTGA CTGTGTACAA CCCGCTGTTC 
TTTGATAAAC TGTACCTTCT GGTCCGAGGC TCCTGCTTCC ACTGCCACTT GCTGACCTGC 
CACCGCTCGG TTATCCACTT ACTGCTCAAC CAGCTCAAGG TCCTGGATCG GGGGGCTGTG 
CATGAAGTCC ACGAGCTGGA GAATGTCCTG CACCGGCTGC TGGAGGCCAA TGCGGCTGCC 
ACTGGTAATG AGATCCGGGA GGAGCTGGGA GACCACACCA GGAAAGTGCT TAAAAAGTAT 
AAGAAGCAGC CCGGGGGCAT TTCTGTGAAA AATACCTGTG TGACCAGAAA CCAGCTGATC 
GGGAACTTCT GGAAGACGCA AATGGTTTCG GCAAAGTGTC CCAACTGCAA GGCTCCACGA 
TCAGTTGTAA GGAAAGAGCA CAACAGCAAG CTGACCATAA TTATGTCTTC CAAATCAAGC 
GCCTCTTCTA AAGGGGGCAA CAAGCCGGCA GAGGGGGACA CAGAAGTGGC TGACAGCTCC 
CAGCTAGCCA AACGGGGATA TCTGACTCCC AACTCCACCA AAGAACTGAT CAAGGCGCTG 
TGGGAGAATG AAGGCTTTTT CCTGCAGCAT CTCTTTTCTG GAATAACAGG CAATGGGGAA 
TCGGCCTTTA CTCCTGACAC GTTTTTCCTA GACATTATTG TTGTCCCTCC CTCAAGGTAC 
CGACCAGTCA ATCGCCTTGG GGATCAGGTT TTCACCAATG GTCACACAGT TAACCTGCAG 
GCTGTCCTCA AAGACGTCTC CATGATACAG AAACTTCTGG CTGTGATGGC TCAGGATCAG 
GAGCAGATGC CGGAGGTCAC CGAATCAGGG ATGAGCTTGA AGGAAGGCGA AGTCACGGAG 
AATATCGACC GCTCTTTTCT TGCCACTTTA ATGGGCCAGT CCCTTACGGA TAAGCTGTAC 
AACTCCTGGG TCCGGCTGCA GAGCCACGTC AATATTGTCT TCGATAGCGA CATGGACAAG 
CTCATGACTG AAAAGTACCC GGGAATCCGC CAGGTCCTGG AGAAGAAAGA AGGTCTGTTT 
CGTAAGCACA TGATGGGGAA GCGAGTGGAT TATGCAGCTC GCTCTGTTAT CTGCCCCGAC 
ATGTACATCA ACACCAATGA AATTGGAATC CCTATGGTGT TTGCCACTAA GCTGACGTAC 
CCCCAGCCGG TCACTCCCTG GAATGTGCAA GAGTTGAGAC AGGCTGTAAT GAACGGACCC 
AACGTCCATC CAGGTGCCTC CATGGTGATC AGTGAGGATG GCTCTCGCAC CATATTGAAC 
TCTTCCAATG TATCCCAGAG GGAAGCTGTA GCAAAGCAAC TACTCACCCC ATCCACTGGC 
GCACAGACTC TGGCTGCTAA AATTGTGTGT CGCCATGTGA AAAATGGGGA TGTGCTTCTC 
CTAAACCGGC AGCCCACCCT TCACAGGCCC TCCATCCAGG CCCACAAAGC CCGTATTCTC 
CCAGGGGAGA AGGTGCTCCG GCTGCACTAC GCCAACTGTA AGGCGTACAA TGCTGACTTC 
GATGGGGATG AAATGAACGC GCATTTCCCC CAAAGCGAGC TGGGCCGGGC TGAAGCCTAC 
AGCTTAGCAT GTACAGATGA GCAGTACTTG GTGCCTAAGG ATGGTACACC CCTTGCCGGC 
CTAATTCAAG ATCATATGGT GTCCGGCGCA AACATGACCA TCCGCGGCTG CTTTTTCACC 
CGGGAACAGT ATATGGAATT GGTTTATCAA GGGCTGACAG ATAAGCGAGG GCGCGTAAAA 
GTCCTCCCAC CAGCTATCCT GAAACCCTAC CCACTTTGGA CAGGAAAGCA GGTTGTATCT 
ACGTTACTAA TCAACGTAAT CCCTGAAAAT CTGATTCCCC TCAACCTGAC CGGAAAAGCC 
AAAATCAGCG GGAAAGCTTG GGTGAAGGAG CAGCCTCGGC CTATTCCGGG ATTCTCGCCG 
GACTCCATGT GCGAGTCTCA GGTAATCATT AGAGACGGGG AATTGCTTTG TGGCGTCCTG 
GACAAAGCCC ACTATGGGAG CTCTTCCTTC GGCTTGGTGC ACTGCTGCTA TGAGCTGTAC 
GGAGGCGAGA CCAGCGGCAA ACTGCTGACT TGCTTGGCGA GGCTCTTCAC TGCCTACCTC 
CAGCTCTACA GAGGGTTCAC TCTGGGAGTT GAAGACATTC TTGTGCTACC CTATGCAGAT 
GCCCAGAGAC TGAATATTGT ACAGCAATCA TTGCATTGTG GTGGGAAGGC TATCAGAGCG 
GCTTTCAACT TGTCGGAAGC GGCGTCAGAC GAAGAAGCGC ACGGGAAGTG GCAGGATGCC 
CATCTCAGCA AAGACCAGAG AGATTTCAAC ATGGTGGATC TGAAATTCAA GGAGGAAGTG 
AACCAGTTCA ACAACGAAAT CAATAAGGTG TGCATGCCTT TCGGCCTCCA TCGACCCTTC 
CCAGAGAACA ACCTGCAGCT GATGGTTCAG TCAGGAGCCA AAGGATCCAC TGTAAATACC 
ATGCAGATTT CCTGTTTATT GGGCCAGATT GAGCTGGAGG GGCGGAGGCC CCCACTGATG 
CCTTCTGGGA GGTCATTACC TTGTTTCCAG CCATATGATT TTAGCCCACG ATCTGGAGGA 
TTTGTTACTG GCCGATTTCT TACTGGCATC AAACCTCCTG AGTTCTTCTT TCATTGCATG 
GCAGGGAGGG AAGGATTGGT GGACACGGCT GTGAAAACTA GTCGCTCGGG ATATCTGCAG 
AGGTGCATTA TTAAACATCT GGAGGGTCTT GTGGTTCAGT ATGACCTGAC GGTGCGAGAC 
AGCGATGGCA GTGTCATTCA GTTCCTGTAC GGAGAGGACG GCCTCGATGT GCCCAAGACA 
CAGTTTCTCC AGCCCAAGCA GTTTCCATTC ATAGCAGAGA ACTACGAGGT TATCAAGCAG 
TCCAAGCACT TGGATGAAGT TCTATCCAGG GTGAACACAG AGCAAGCAGA GCAGCACAAT 
CATGCCATCT ATAGGTGGAA AGCAAGGCAC CCCAACCTCT CCCAGCGAAT CAGCCCCTTC 
CTGCTCTACT CTCAGAAGCA AATGGCAGCT GCAAAAGCAT CGTGTCCTAA TGAGCTGGAT 
GAGAATGGCA GGGATCTCGC ATCCAGAAAG GTATTGGAGA GCTGGTATGC GGCGAATGAG 
AAGACACGTA GCAAATACGT GAGAAGGTCG TCTACTCTCC CTGATCCCTG CCTGGCAGTG 
TGGCGCCCAG ATCTCTACTT GGGATCCGTA TCTGAGAACT TCCAGAGCAA AATGGCGCAG 
TATGGCTGTC TGTGGGAGGA GCAGAGGGAC TCCAGCCAGG GGATGTCCTC GCTGACATTT 
GACAGCTTAA AAACCTTGCT GCACCTAAAG TGGCAGAGAG CGCTGTGTGA CCCCGGGGAT 
GCGGTGGGGC TTCTGGCAGC TCAGAGCATC GGAGAGCCCT CCACACAGAT GACGCTGAAC 
ACTTTCCACT TTGCTGGCAG AGGAGAAATG AACGTCACTC TGGGAATTCC TAGGCTTCGG 
GAGATCCTGA TGGTTGCAAG CGCCAACATA AAGACGCCCA TGATGAGCGT GCCGGTTCTG 
AACACTAAGA AAGCTGTGAA GAAGGTGAAG CAACTGAAAA AGAAGCTAAC GCGAGTGTGC 
CTGGCAGAGG TGATACACAA ACTGGACGTG GAGGAGACGT TCCGTATCGA GGAGAAGTCA 
CAGAAGTACC AAGTGTACAA GATCCGCTTC CATTTCCTAC CCCACGAGTA CTATCGGCAG 
GAGAAGCTCC TGCGGCCCGA GGGGGTCCTG CGCTTCATGG AAACAAGGTT CTTCAAAATC 
ATGTTTGATG CCATAAAAAG GAAAAGCTCC AAGCTTGCTT CTTTCCGCGA AGTGGGCACC 
CGAAGGGCCA CTCAGAAGGA CCATGGGGAT GGGGAAGACC AAGGAAACCA GGAAAGCCAG 
CCCGTGGAGA ATGAAGAAGA GCAAGTCAAT GCGGATGATG ATGATGATGA TGCAGAGGAA 
GGTGATGCTG ATGCCACTGA TAAAAAAAGA AGAAAAAAAC AAGAGGAGGA GGTGGACTAT 
GAGAGTGAGG AAGAGGAGGC TGAAGAAGAC AATGAGGAGG AGGTGGGGTC TCCCGAGGCA 
GGAGAGGAGA CATCTGCTGA TGTGAAAGAG AACACTGGGG ATATTGTGGA TGAAATAGAG 
GAGAACTCCC ACTCCCCCGT ACCTGCCAGG AGACCTCAAA GTGGACTTGC CAAGGAGGTT 
GTGGCCGAAA ACAGAATAAA CGATGTACTT AGCCTCAATT CCGCTATAGA AGATTACTGC 
TATGATACAG AGAACAGCTT GTGGTGTGAG ATCACAATCA AACTGCAGCT GATGAAGATC 
CACTTCGATA TCTGTTCCCT CGTGTCCTCC CTCGCCCACA GTGCTATAGT CCATGAGACC 
AGCGGCATCA CCCGGTGCCT CTTGAATGAT ACGGCCAACA AGCAAGGGGA CAAAGAGCTG 
ATTCTCAACA CAGAGGGGAT CAATCTGTAT GAACTTTTCA AGTATTCCAA TATCCTGGAG 
CTGAGGAGGC TTTATTCCAA TGATATACAC ACCATGGCAA AAACCTTTGG CATTGAGGCT 
GCTTTACGGG TGGTAGAGAG GGAGATTAAA GACGTGTTTG CGGTTTATGG TATCGAAGTG 
GATCCCCGGC ACCTCTCGCT GGTTGCGGAT TACATGTGCT TCGAGGGTAC CTACAAGCCT 
CTCAATCGGA TCGGGATGCA GTCTAGCTCC TCGCCCTTCC AGCAGATGAC ATTTGAAACT 
AGCTATAAAT TTCTAAAGGA TGCCACCATG AATGGCGCTC GGGATGACCT GCAGTCTCCG 
TCGGCGTGCT TAGTGGTTGG AAAGGTTGTA AAAGGAGGCA CTGGTTTATT CGACCTTAAG 
CAGCCCATGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751156.1
CDS	60..5414
Translation	MTVGSLRAVPLAEGGGGRSHGARAGLLHLSGSWYVPLPGPHVWPAEVREAERFEGTGQAAQCGLPCRAMLISKDVPWRRLQGVSFGCYSAEEIRKLSVKALTNPHYMDNLGNPMMNGLYDLALGPADGKEVCSTCAQDFNNCPGHLGHIELPLTVYNPLFFDKLYLLVRGSCFHCHLLTCHRSVIHLLLNQLKVLDRGAVHEVHELENVLHRLLEANAAATGNEIREELGDHTRKVLKKYKKQPGGISVKNTCVTRNQLIGNFWKTQMVSAKCPNCKAPRSVVRKEHNSKLTIIMSSKSSASSKGGNKPAEGDTEVADSSQLAKRGYLTPNSTKELIKALWENEGFFLQHLFSGITGNGESAFTPDTFFLDIIVVPPSRYRPVNRLGDQVFTNGHTVNLQAVLKDVSMIQKLLAVMAQDQEQMPEVTESGMSLKEGEVTENIDRSFLATLMGQSLTDKLYNSWVRLQSHVNIVFDSDMDKLMTEKYPGIRQVLEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVMNGPNVHPGASMVISEDGSRTILNSSNVSQREAVAKQLLTPSTGAQTLAAKIVCRHVKNGDVLLLNRQPTLHRPSIQAHKARILPGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYSLACTDEQYLVPKDGTPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYQGLTDKRGRVKVLPPAILKPYPLWTGKQVVSTLLINVIPENLIPLNLTGKAKISGKAWVKEQPRPIPGFSPDSMCESQVIIRDGELLCGVLDKAHYGSSSFGLVHCCYELYGGETSGKLLTCLARLFTAYLQLYRGFTLGVEDILVLPYADAQRLNIVQQSLHCGGKAIRAAFNLSEAASDEEAHGKWQDAHLSKDQRDFNMVDLKFKEEVNQFNNEINKVCMPFGLHRPFPENNLQLMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMPSGRSLPCFQPYDFSPRSGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVIQFLYGEDGLDVPKTQFLQPKQFPFIAENYEVIKQSKHLDEVLSRVNTEQAEQHNHAIYRWKARHPNLSQRISPFLLYSQKQMAAAKASCPNELDENGRDLASRKVLESWYAANEKTRSKYVRRSSTLPDPCLAVWRPDLYLGSVSENFQSKMAQYGCLWEEQRDSSQGMSSLTFDSLKTLLHLKWQRALCDPGDAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKAVKKVKQLKKKLTRVCLAEVIHKLDVEETFRIEEKSQKYQVYKIRFHFLPHEYYRQEKLLRPEGVLRFMETRFFKIMFDAIKRKSSKLASFREVGTRRATQKDHGDGEDQGNQESQPVENEEEQVNADDDDDDAEEGDADATDKKRRKKQEEEVDYESEEEEAEEDNEEEVGSPEAGEETSADVKENTGDIVDEIEENSHSPVPARRPQSGLAKEVVAENRINDVLSLNSAIEDYCYDTENSLWCEITIKLQLMKIHFDICSLVSSLAHSAIVHETSGITRCLLNDTANKQGDKELILNTEGINLYELFKYSNILELRRLYSNDIHTMAKTFGIEAALRVVEREIKDVFAVYGIEVDPRHLSLVADYMCFEGTYKPLNRIGMQSSSSPFQQMTFETSYKFLKDATMNGARDDLQSPSACLVVGKVVKGGTGLFDLKQPMK

Target ORF information:

RefSeq Version	XM_031895296.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (RNA) I polypeptide A (polr1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895296.1

ORF Insert Sequence:

ATGACAGTAG GAAGTCTGCG GGCTGTACCA CTGGCTGAGG GGGGGGGGGG CAGGTCGCAC 
GGCGCTAGAG CAGGACTCCT CCACTTGTCA GGCAGTTGGT ACGTTCCACT TCCGGGTCCC 
CACGTGTGGC CGGCAGAAGT CCGGGAGGCG GAGAGGTTTG AGGGAACCGG GCAAGCGGCG 
CAGTGTGGCC TCCCCTGCCG GGCAATGCTC ATCTCTAAGG ACGTGCCATG GCGGCGCCTG 
CAAGGCGTCT CTTTCGGCTG TTACTCAGCG GAGGAGATCA GGAAGCTGAG TGTGAAAGCC 
CTGACCAACC CTCACTATAT GGACAACCTG GGCAATCCGA TGATGAACGG CCTGTATGAT 
CTGGCCCTGG GTCCCGCTGA CGGCAAAGAA GTGTGCTCCA CCTGCGCTCA GGACTTCAAT 
AACTGCCCCG GGCACCTGGG GCACATCGAG CTGCCCCTGA CTGTGTACAA CCCGCTGTTC 
TTTGATAAAC TGTACCTTCT GGTCCGAGGC TCCTGCTTCC ACTGCCACTT GCTGACCTGC 
CACCGCTCGG TTATCCACTT ACTGCTCAAC CAGCTCAAGG TCCTGGATCG GGGGGCTGTG 
CATGAAGTCC ACGAGCTGGA GAATGTCCTG CACCGGCTGC TGGAGGCCAA TGCGGCTGCC 
ACTGGTAATG AGATCCGGGA GGAGCTGGGA GACCACACCA GGAAAGTGCT TAAAAAGTAT 
AAGAAGCAGC CCGGGGGCAT TTCTGTGAAA AATACCTGTG TGACCAGAAA CCAGCTGATC 
GGGAACTTCT GGAAGACGCA AATGGTTTCG GCAAAGTGTC CCAACTGCAA GGCTCCACGA 
TCAGTTGTAA GGAAAGAGCA CAACAGCAAG CTGACCATAA TTATGTCTTC CAAATCAAGC 
GCCTCTTCTA AAGGGGGCAA CAAGCCGGCA GAGGGGGACA CAGAAGTGGC TGACAGCTCC 
CAGCTAGCCA AACGGGGATA TCTGACTCCC AACTCCACCA AAGAACTGAT CAAGGCGCTG 
TGGGAGAATG AAGGCTTTTT CCTGCAGCAT CTCTTTTCTG GAATAACAGG CAATGGGGAA 
TCGGCCTTTA CTCCTGACAC GTTTTTCCTA GACATTATTG TTGTCCCTCC CTCAAGGTAC 
CGACCAGTCA ATCGCCTTGG GGATCAGGTT TTCACCAATG GTCACACAGT TAACCTGCAG 
GCTGTCCTCA AAGACGTCTC CATGATACAG AAACTTCTGG CTGTGATGGC TCAGGATCAG 
GAGCAGATGC CGGAGGTCAC CGAATCAGGG ATGAGCTTGA AGGAAGGCGA AGTCACGGAG 
AATATCGACC GCTCTTTTCT TGCCACTTTA ATGGGCCAGT CCCTTACGGA TAAGCTGTAC 
AACTCCTGGG TCCGGCTGCA GAGCCACGTC AATATTGTCT TCGATAGCGA CATGGACAAG 
CTCATGACTG AAAAGTACCC GGGAATCCGC CAGGTCCTGG AGAAGAAAGA AGGTCTGTTT 
CGTAAGCACA TGATGGGGAA GCGAGTGGAT TATGCAGCTC GCTCTGTTAT CTGCCCCGAC 
ATGTACATCA ACACCAATGA AATTGGAATC CCTATGGTGT TTGCCACTAA GCTGACGTAC 
CCCCAGCCGG TCACTCCCTG GAATGTGCAA GAGTTGAGAC AGGCTGTAAT GAACGGACCC 
AACGTCCATC CAGGTGCCTC CATGGTGATC AGTGAGGATG GCTCTCGCAC CATATTGAAC 
TCTTCCAATG TATCCCAGAG GGAAGCTGTA GCAAAGCAAC TACTCACCCC ATCCACTGGC 
GCACAGACTC TGGCTGCTAA AATTGTGTGT CGCCATGTGA AAAATGGGGA TGTGCTTCTC 
CTAAACCGGC AGCCCACCCT TCACAGGCCC TCCATCCAGG CCCACAAAGC CCGTATTCTC 
CCAGGGGAGA AGGTGCTCCG GCTGCACTAC GCCAACTGTA AGGCGTACAA TGCTGACTTC 
GATGGGGATG AAATGAACGC GCATTTCCCC CAAAGCGAGC TGGGCCGGGC TGAAGCCTAC 
AGCTTAGCAT GTACAGATGA GCAGTACTTG GTGCCTAAGG ATGGTACACC CCTTGCCGGC 
CTAATTCAAG ATCATATGGT GTCCGGCGCA AACATGACCA TCCGCGGCTG CTTTTTCACC 
CGGGAACAGT ATATGGAATT GGTTTATCAA GGGCTGACAG ATAAGCGAGG GCGCGTAAAA 
GTCCTCCCAC CAGCTATCCT GAAACCCTAC CCACTTTGGA CAGGAAAGCA GGTTGTATCT 
ACGTTACTAA TCAACGTAAT CCCTGAAAAT CTGATTCCCC TCAACCTGAC CGGAAAAGCC 
AAAATCAGCG GGAAAGCTTG GGTGAAGGAG CAGCCTCGGC CTATTCCGGG ATTCTCGCCG 
GACTCCATGT GCGAGTCTCA GGTAATCATT AGAGACGGGG AATTGCTTTG TGGCGTCCTG 
GACAAAGCCC ACTATGGGAG CTCTTCCTTC GGCTTGGTGC ACTGCTGCTA TGAGCTGTAC 
GGAGGCGAGA CCAGCGGCAA ACTGCTGACT TGCTTGGCGA GGCTCTTCAC TGCCTACCTC 
CAGCTCTACA GAGGGTTCAC TCTGGGAGTT GAAGACATTC TTGTGCTACC CTATGCAGAT 
GCCCAGAGAC TGAATATTGT ACAGCAATCA TTGCATTGTG GTGGGAAGGC TATCAGAGCG 
GCTTTCAACT TGTCGGAAGC GGCGTCAGAC GAAGAAGCGC ACGGGAAGTG GCAGGATGCC 
CATCTCAGCA AAGACCAGAG AGATTTCAAC ATGGTGGATC TGAAATTCAA GGAGGAAGTG 
AACCAGTTCA ACAACGAAAT CAATAAGGTG TGCATGCCTT TCGGCCTCCA TCGACCCTTC 
CCAGAGAACA ACCTGCAGCT GATGGTTCAG TCAGGAGCCA AAGGATCCAC TGTAAATACC 
ATGCAGATTT CCTGTTTATT GGGCCAGATT GAGCTGGAGG GGCGGAGGCC CCCACTGATG 
CCTTCTGGGA GGTCATTACC TTGTTTCCAG CCATATGATT TTAGCCCACG ATCTGGAGGA 
TTTGTTACTG GCCGATTTCT TACTGGCATC AAACCTCCTG AGTTCTTCTT TCATTGCATG 
GCAGGGAGGG AAGGATTGGT GGACACGGCT GTGAAAACTA GTCGCTCGGG ATATCTGCAG 
AGGTGCATTA TTAAACATCT GGAGGGTCTT GTGGTTCAGT ATGACCTGAC GGTGCGAGAC 
AGCGATGGCA GTGTCATTCA GTTCCTGTAC GGAGAGGACG GCCTCGATGT GCCCAAGACA 
CAGTTTCTCC AGCCCAAGCA GTTTCCATTC ATAGCAGAGA ACTACGAGGT TATCAAGCAG 
TCCAAGCACT TGGATGAAGT TCTATCCAGG GTGAACACAG AGCAAGCAGA GCAGCACAAT 
CATGCCATCT ATAGGTGGAA AGCAAGGCAC CCCAACCTCT CCCAGCGAAT CAGCCCCTTC 
CTGCTCTACT CTCAGAAGCA AATGGCAGCT GCAAAAGCAT CGTGTCCTAA TGAGCTGGAT 
GAGAATGGCA GGGATCTCGC ATCCAGAAAG GTATTGGAGA GCTGGTATGC GGCGAATGAG 
AAGACACGTA GCAAATACGT GAGAAGGTCG TCTACTCTCC CTGATCCCTG CCTGGCAGTG 
TGGCGCCCAG ATCTCTACTT GGGATCCGTA TCTGAGAACT TCCAGAGCAA AATGGCGCAG 
TATGGCTGTC TGTGGGAGGA GCAGAGGGAC TCCAGCCAGG GGATGTCCTC GCTGACATTT 
GACAGCTTAA AAACCTTGCT GCACCTAAAG TGGCAGAGAG CGCTGTGTGA CCCCGGGGAT 
GCGGTGGGGC TTCTGGCAGC TCAGAGCATC GGAGAGCCCT CCACACAGAT GACGCTGAAC 
ACTTTCCACT TTGCTGGCAG AGGAGAAATG AACGTCACTC TGGGAATTCC TAGGCTTCGG 
GAGATCCTGA TGGTTGCAAG CGCCAACATA AAGACGCCCA TGATGAGCGT GCCGGTTCTG 
AACACTAAGA AAGCTGTGAA GAAGGTGAAG CAACTGAAAA AGAAGCTAAC GCGAGTGTGC 
CTGGCAGAGG TGATACACAA ACTGGACGTG GAGGAGACGT TCCGTATCGA GGAGAAGTCA 
CAGAAGTACC AAGTGTACAA GATCCGCTTC CATTTCCTAC CCCACGAGTA CTATCGGCAG 
GAGAAGCTCC TGCGGCCCGA GGGGGTCCTG CGCTTCATGG AAACAAGGTT CTTCAAAATC 
ATGTTTGATG CCATAAAAAG GAAAAGCTCC AAGCTTGCTT CTTTCCGCGA AGTGGGCACC 
CGAAGGGCCA CTCAGAAGGA CCATGGGGAT GGGGAAGACC AAGGAAACCA GGAAAGCCAG 
CCCGTGGAGA ATGAAGAAGA GCAAGTCAAT GCGGATGATG ATGATGATGA TGCAGAGGAA 
GGTGATGCTG ATGCCACTGA TAAAAAAAGA AGAAAAAAAC AAGAGGAGGA GGTGGACTAT 
GAGAGTGAGG AAGAGGAGGC TGAAGAAGAC AATGAGGAGG AGGTGGGGTC TCCCGAGGCA 
GGAGAGGAGA CATCTGCTGA TGTGAAAGAG AACACTGGGG ATATTGTGGA TGAAATAGAG 
GAGAACTCCC ACTCCCCCGT ACCTGCCAGG AGACCTCAAA GTGGACTTGC CAAGGAGGTT 
GTGGCCGAAA ACAGAATAAA CGATGTACTT AGCCTCAATT CCGCTATAGA AGATTACTGC 
TATGATACAG AGAACAGCTT GTGGTGTGAG ATCACAATCA AACTGCAGCT GATGAAGATC 
CACTTCGATA TCTGTTCCCT CGTGTCCTCC CTCGCCCACA GTGCTATAGT CCATGAGACC 
AGCGGCATCA CCCGGTGCCT CTTGAATGAT ACGGCCAACA AGCAAGGGGA CAAAGAGCTG 
ATTCTCAACA CAGAGGGGAT CAATCTGTAT GAACTTTTCA AGTATTCCAA TATCCTGGAG 
CTGAGGAGGC TTTATTCCAA TGATATACAC ACCATGGCAA AAACCTTTGG CATTGAGGCT 
GCTTTACGGG TGGTAGAGAG GGAGATTAAA GACGTGTTTG CGGTTTATGG TATCGAAGTG 
GATCCCCGGC ACCTCTCGCT GGTTGCGGAT TACATGTGCT TCGAGGGTAC CTACAAGCCT 
CTCAATCGGA TCGGGATGCA GTCTAGCTCC TCGCCCTTCC AGCAGATGAC ATTTGAAACT 
AGCTATAAAT TTCTAAAGGA TGCCACCATG AATGGCGCTC GGGATGACCT GCAGTCTCCG 
TCGGCGTGCT TAGTGGTTGG AAAGGTTGTA AAAGGAGGCA CTGGTTTATT CGACCTTAAG 
CAGCCCATGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

polr1a ( XM_012955748.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811202.1 Xenopus tropicalis polymerase (RNA) I polypeptide A (polr1a), mRNA.

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CloneID	OXa32236
Clone ID Related Accession (Same CDS sequence)	XM_012955748.2
Accession Version	XM_012955748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) and transcript sequence (BC166364.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 153 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTCATCT CTAAGGACGT GCCATGGCGG CGCCTGCAAG GCGTCTCTTT CGGCTGTTAC 
TCAGCGGAGG AGATCAGGAA GCTGAGTGTG AAAGCCCTGA CCAACCCTCA CTATATGGAC 
AACCTGGGCA ATCCGATGAT GAACGGCCTG TATGATCTGG CCCTGGGTCC CGCTGACGGC 
AAAGAAGTGT GCTCCACCTG CGCTCAGGAC TTCAATAACT GCCCCGGGCA CCTGGGGCAC 
ATCGAGCTGC CCCTGACTGT GTACAACCCG CTGTTCTTTG ATAAACTGTA CCTTCTGGTC 
CGAGGCTCCT GCTTCCACTG CCACTTGCTG ACCTGCCACC GCTCGGTTAT CCACTTACTG 
CTCAACCAGC TCAAGGTCCT GGATCGGGGG GCTGTGCATG AAGTCCACGA GCTGGAGAAT 
GTCCTGCACC GGCTGCTGGA GGCCAATGCG GCTGCCACTG GTAATGAGAT CCGGGAGGAG 
CTGGGAGACC ACACCAGGAA AGTGCTTAAA AAGTATAAGA AGCAGCCCGG GGGCATTTCT 
GTGAAAAATA CCTGTGTGAC CAGAAACCAG CTGATCGGGA ACTTCTGGAA GACGCAAATG 
GTTTCGGCAA AGTGTCCCAA CTGCAAGGCT CCACGATCAG TTGTAAGGAA AGAGCACAAC 
AGCAAGCTGA CCATAATTAT GTCTTCCAAA TCAAGCGCCT CTTCTAAAGG GGGCAACAAG 
CCGGCAGAGG GGGACACAGA AGTGGCTGAC AGCTCCCAGC TAGCCAAACG GGGATATCTG 
ACTCCCAACT CCACCAAAGA ACTGATCAAG GCGCTGTGGG AGAATGAAGG CTTTTTCCTG 
CAGCATCTCT TTTCTGGAAT AACAGGCAAT GGGGAATCGG CCTTTACTCC TGACACGTTT 
TTCCTAGACA TTATTGTTGT CCCTCCCTCA AGGTACCGAC CAGTCAATCG CCTTGGGGAT 
CAGGTTTTCA CCAATGGTCA CACAGTTAAC CTGCAGGCTG TCCTCAAAGA CGTCTCCATG 
ATACAGAAAC TTCTGGCTGT GATGGCTCAG GATCAGGAGC AGATGCCGGA GGTCACCGAA 
TCAGGGATGA GCTTGAAGGA AGGCGAAGTC ACGGAGAATA TCGACCGCTC TTTTCTTGCC 
ACTTTAATGG GCCAGTCCCT TACGGATAAG CTGTACAACT CCTGGGTCCG GCTGCAGAGC 
CACGTCAATA TTGTCTTCGA TAGCGACATG GACAAGCTCA TGACTGAAAA GTACCCGGGA 
ATCCGCCAGG TCCTGGAGAA GAAAGAAGGT CTGTTTCGTA AGCACATGAT GGGGAAGCGA 
GTGGATTATG CAGCTCGCTC TGTTATCTGC CCCGACATGT ACATCAACAC CAATGAAATT 
GGAATCCCTA TGGTGTTTGC CACTAAGCTG ACGTACCCCC AGCCGGTCAC TCCCTGGAAT 
GTGCAAGAGT TGAGACAGGC TGTAATGAAC GGACCCAACG TCCATCCAGG TGCCTCCATG 
GTGATCAGTG AGGATGGCTC TCGCACCATA TTGAACTCTT CCAATGTATC CCAGAGGGAA 
GCTGTAGCAA AGCAACTACT CACCCCATCC ACTGGCGCAC AGACTCTGGC TGCTAAAATT 
GTGTGTCGCC ATGTGAAAAA TGGGGATGTG CTTCTCCTAA ACCGGCAGCC CACCCTTCAC 
AGGCCCTCCA TCCAGGCCCA CAAAGCCCGT ATTCTCCCAG GGGAGAAGGT GCTCCGGCTG 
CACTACGCCA ACTGTAAGGC GTACAATGCT GACTTCGATG GGGATGAAAT GAACGCGCAT 
TTCCCCCAAA GCGAGCTGGG CCGGGCTGAA GCCTACAGCT TAGCATGTAC AGATGAGCAG 
TACTTGGTGC CTAAGGATGG TACACCCCTT GCCGGCCTAA TTCAAGATCA TATGGTGTCC 
GGCGCAAACA TGACCATCCG CGGCTGCTTT TTCACCCGGG AACAGTATAT GGAATTGGTT 
TATCAAGGGC TGACAGATAA GCGAGGGCGC GTAAAAGTCC TCCCACCAGC TATCCTGAAA 
CCCTACCCAC TTTGGACAGG AAAGCAGGTT GTATCTACGT TACTAATCAA CGTAATCCCT 
GAAAATCTGA TTCCCCTCAA CCTGACCGGA AAAGCCAAAA TCAGCGGGAA AGCTTGGGTG 
AAGGAGCAGC CTCGGCCTAT TCCGGGATTC TCGCCGGACT CCATGTGCGA GTCTCAGGTA 
ATCATTAGAG ACGGGGAATT GCTTTGTGGC GTCCTGGACA AAGCCCACTA TGGGAGCTCT 
TCCTTCGGCT TGGTGCACTG CTGCTATGAG CTGTACGGAG GCGAGACCAG CGGCAAACTG 
CTGACTTGCT TGGCGAGGCT CTTCACTGCC TACCTCCAGC TCTACAGAGG GTTCACTCTG 
GGAGTTGAAG ACATTCTTGT GCTACCCTAT GCAGATGCCC AGAGACTGAA TATTGTACAG 
CAATCATTGC ATTGTGGTGG GAAGGCTATC AGAGCGGCTT TCAACTTGTC GGAAGCGGCG 
TCAGACGAAG AAGCGCACGG GAAGTGGCAG GATGCCCATC TCAGCAAAGA CCAGAGAGAT 
TTCAACATGG TGGATCTGAA ATTCAAGGAG GAAGTGAACC AGTTCAACAA CGAAATCAAT 
AAGGTGTGCA TGCCTTTCGG CCTCCATCGA CCCTTCCCAG AGAACAACCT GCAGCTGATG 
GTTCAGTCAG GAGCCAAAGG ATCCACTGTA AATACCATGC AGATTTCCTG TTTATTGGGC 
CAGATTGAGC TGGAGGGGCG GAGGCCCCCA CTGATGCCTT CTGGGAGGTC ATTACCTTGT 
TTCCAGCCAT ATGATTTTAG CCCACGATCT GGAGGATTTG TTACTGGCCG ATTTCTTACT 
GGCATCAAAC CTCCTGAGTT CTTCTTTCAT TGCATGGCAG GGAGGGAAGG ATTGGTGGAC 
ACGGCTGTGA AAACTAGTCG CTCGGGATAT CTGCAGAGGT GCATTATTAA ACATCTGGAG 
GGTCTTGTGG TTCAGTATGA CCTGACGGTG CGAGACAGCG ATGGCAGTGT CATTCAGTTC 
CTGTACGGAG AGGACGGCCT CGATGTGCCC AAGACACAGT TTCTCCAGCC CAAGCAGTTT 
CCATTCATAG CAGAGAACTA CGAGGTTATC AAGCAGTCCA AGCACTTGGA TGAAGTTCTA 
TCCAGGGTGA ACACAGAGCA AGCAGAGCAG CACAATCATG CCATCTATAG GTGGAAAGCA 
AGGCACCCCA ACCTCTCCCA GCGAATCAGC CCCTTCCTGC TCTACTCTCA GAAGCAAATG 
GCAGCTGCAA AAGCATCGTG TCCTAATGAG CTGGATGAGA ATGGCAGGGA TCTCGCATCC 
AGACAGGTAT TGGAGAGCTG GTATGCGGCG AATGAGAAGA CACGTAGCAA ATACGTGAGA 
AGGTCGTCTA CTCTCCCTGA TCCCTGCCTG GCAGTGTGGC GCCCAGATCT CTACTTGGGA 
TCCGTATCTG AGAACTTCCA GAGCAAAATG GCGCAGTATG GCTGTCTGTG GGAGGAGCAG 
AGGGACTCCA GCCATGGGAT GTCCTCGCTG ACATTTGACA GCTTAAAAAC CTTGCTGCAC 
CTAAAGTGGC AGAGAGCGCT GTGTGACCCC GGGGATGCGG TGGGGCTTCT GGCAGCTCAG 
AGCATCGGAG AGCCCTCCAC ACAGATGACG CTGAACACTT TCCACTTTGC TGGCAGAGGA 
GAAATGAACG TCACTCTGGG AATTCCTAGG CTTCGGGAGA TCCTGATGGT TGCAAGCGCC 
AACATAAAGA CGCCCATGAT GAGCGTGCCG GTTCTGAACA CTAAGAAAGC TGTGAAGAAG 
GTGAAGCAAC TGAAAAAGAA GCTAACGCGA GTGTGCCTGG CAGAGGTGAT ACACAAACTG 
GACGTGGAGG AGACGTTCCG TATCGAGGAG AAGTCACAGA AGTACCAAGT GTACAAGATC 
CGCTTCCATT TCCTACCCCA CGAGTACTAT CGGCAGGAGA AGCTCCTGCG GCCCGAGGGG 
GTCCTGCGCT TCATGGAAAC AAGGTTCTTC AAAATCATGT TTGATGCCAT AAAAAGGAAA 
AGCTCCAAGC TTGCTTCTTT CCGCGAAGTG GGCACCCGAA GGGCCACTCA GAAGGACCAT 
GGGGATGGGG AAGACCAAGG AAACCAGGAA AGCCAGCCCG TGGAGAATGA AGAAGAGCAA 
GTCAATGCGG ATGATGATGA TGATGATGCA GAGGAAGGTG ATGCTGATGC CACTGATAAA 
AAAAGAAGAA AAAAACAAGA GGAGGAGGTG GACTATGAGA GTGAGGAAGA GGAGGCTGAA 
GAAGACAATG AGGAGGAGGT GGGGTCTCCC GAGGCAGGAG AGGAGACATC TGCTGATGTG 
AAAGAGAACA CTGGGGATAT TGTGGATGAA ATAGAGGAGA ACTCCCACTC CCCCGTACCT 
GCCAGGAGAC CTCAAAGTGG ACTTGCCAAG GAGGTTGTGG CCGAAAACAG AATAAACGAT 
GTACTTAGCC TCAATTCCGC TATAGAAGAT TACTGCTATG ATACAGAGAA CAGCTTGTGG 
TGTGAGATCA CAATCAAACT GCAGCTGATG AAGATCCACT TCGATATCTG TTCCCTCGTG 
TCCTCCCTCG CCCACAGTGC TATAGTCCAT GAGACCAGCG GCATCACCCG GTGCCTCTTG 
AATGATACGG CCAACAAGCA AGGGGACAAA GAGCTGATTC TCAACACAGA GGGGATCAAT 
CTGTATGAAC TTTTCAAGTA TTCCAATATC CTGGAGCTGA GGAGGCTTTA TTCCAATGAT 
ATACACACCA TGGCAAAAAC CTTTGGCATT GAGGCTGCTT TACGGGTGGT AGAGAGGGAG 
ATTAAAGACG TGTTTGCGGT TTATGGTATC GAAGTGGATC CCCGGCACCT CTCGCTGGTT 
GCGGATTACA TGTGCTTCGA GGGTACCTAC AAGCCTCTCA ATCGGATCGG GATGCAGTCT 
AGCTCCTCGC CCTTCCAGCA GATGACATTT GAAACTAGCT ATAAATTTCT AAAGGATGCC 
ACCATGAATG GCGCTCGGGA TGACCTGCAG TCTCCGTCGG CGTGCTTAGT GGTTGGAAAG 
GTTGTAAAAG GAGGCACTGG TTTATTCGAC CTTAAGCAGC CCATGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811202.1
CDS	87..5237
Translation	MLISKDVPWRRLQGVSFGCYSAEEIRKLSVKALTNPHYMDNLGNPMMNGLYDLALGPADGKEVCSTCAQDFNNCPGHLGHIELPLTVYNPLFFDKLYLLVRGSCFHCHLLTCHRSVIHLLLNQLKVLDRGAVHEVHELENVLHRLLEANAAATGNEIREELGDHTRKVLKKYKKQPGGISVKNTCVTRNQLIGNFWKTQMVSAKCPNCKAPRSVVRKEHNSKLTIIMSSKSSASSKGGNKPAEGDTEVADSSQLAKRGYLTPNSTKELIKALWENEGFFLQHLFSGITGNGESAFTPDTFFLDIIVVPPSRYRPVNRLGDQVFTNGHTVNLQAVLKDVSMIQKLLAVMAQDQEQMPEVTESGMSLKEGEVTENIDRSFLATLMGQSLTDKLYNSWVRLQSHVNIVFDSDMDKLMTEKYPGIRQVLEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVMNGPNVHPGASMVISEDGSRTILNSSNVSQREAVAKQLLTPSTGAQTLAAKIVCRHVKNGDVLLLNRQPTLHRPSIQAHKARILPGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYSLACTDEQYLVPKDGTPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYQGLTDKRGRVKVLPPAILKPYPLWTGKQVVSTLLINVIPENLIPLNLTGKAKISGKAWVKEQPRPIPGFSPDSMCESQVIIRDGELLCGVLDKAHYGSSSFGLVHCCYELYGGETSGKLLTCLARLFTAYLQLYRGFTLGVEDILVLPYADAQRLNIVQQSLHCGGKAIRAAFNLSEAASDEEAHGKWQDAHLSKDQRDFNMVDLKFKEEVNQFNNEINKVCMPFGLHRPFPENNLQLMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMPSGRSLPCFQPYDFSPRSGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVIQFLYGEDGLDVPKTQFLQPKQFPFIAENYEVIKQSKHLDEVLSRVNTEQAEQHNHAIYRWKARHPNLSQRISPFLLYSQKQMAAAKASCPNELDENGRDLASRQVLESWYAANEKTRSKYVRRSSTLPDPCLAVWRPDLYLGSVSENFQSKMAQYGCLWEEQRDSSHGMSSLTFDSLKTLLHLKWQRALCDPGDAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKAVKKVKQLKKKLTRVCLAEVIHKLDVEETFRIEEKSQKYQVYKIRFHFLPHEYYRQEKLLRPEGVLRFMETRFFKIMFDAIKRKSSKLASFREVGTRRATQKDHGDGEDQGNQESQPVENEEEQVNADDDDDDAEEGDADATDKKRRKKQEEEVDYESEEEEAEEDNEEEVGSPEAGEETSADVKENTGDIVDEIEENSHSPVPARRPQSGLAKEVVAENRINDVLSLNSAIEDYCYDTENSLWCEITIKLQLMKIHFDICSLVSSLAHSAIVHETSGITRCLLNDTANKQGDKELILNTEGINLYELFKYSNILELRRLYSNDIHTMAKTFGIEAALRVVEREIKDVFAVYGIEVDPRHLSLVADYMCFEGTYKPLNRIGMQSSSSPFQQMTFETSYKFLKDATMNGARDDLQSPSACLVVGKVVKGGTGLFDLKQPMK

Target ORF information:

RefSeq Version	XM_012955748.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (RNA) I polypeptide A (polr1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955748.2

ORF Insert Sequence:

ATGCTCATCT CTAAGGACGT GCCATGGCGG CGCCTGCAAG GCGTCTCTTT CGGCTGTTAC 
TCAGCGGAGG AGATCAGGAA GCTGAGTGTG AAAGCCCTGA CCAACCCTCA CTATATGGAC 
AACCTGGGCA ATCCGATGAT GAACGGCCTG TATGATCTGG CCCTGGGTCC CGCTGACGGC 
AAAGAAGTGT GCTCCACCTG CGCTCAGGAC TTCAATAACT GCCCCGGGCA CCTGGGGCAC 
ATCGAGCTGC CCCTGACTGT GTACAACCCG CTGTTCTTTG ATAAACTGTA CCTTCTGGTC 
CGAGGCTCCT GCTTCCACTG CCACTTGCTG ACCTGCCACC GCTCGGTTAT CCACTTACTG 
CTCAACCAGC TCAAGGTCCT GGATCGGGGG GCTGTGCATG AAGTCCACGA GCTGGAGAAT 
GTCCTGCACC GGCTGCTGGA GGCCAATGCG GCTGCCACTG GTAATGAGAT CCGGGAGGAG 
CTGGGAGACC ACACCAGGAA AGTGCTTAAA AAGTATAAGA AGCAGCCCGG GGGCATTTCT 
GTGAAAAATA CCTGTGTGAC CAGAAACCAG CTGATCGGGA ACTTCTGGAA GACGCAAATG 
GTTTCGGCAA AGTGTCCCAA CTGCAAGGCT CCACGATCAG TTGTAAGGAA AGAGCACAAC 
AGCAAGCTGA CCATAATTAT GTCTTCCAAA TCAAGCGCCT CTTCTAAAGG GGGCAACAAG 
CCGGCAGAGG GGGACACAGA AGTGGCTGAC AGCTCCCAGC TAGCCAAACG GGGATATCTG 
ACTCCCAACT CCACCAAAGA ACTGATCAAG GCGCTGTGGG AGAATGAAGG CTTTTTCCTG 
CAGCATCTCT TTTCTGGAAT AACAGGCAAT GGGGAATCGG CCTTTACTCC TGACACGTTT 
TTCCTAGACA TTATTGTTGT CCCTCCCTCA AGGTACCGAC CAGTCAATCG CCTTGGGGAT 
CAGGTTTTCA CCAATGGTCA CACAGTTAAC CTGCAGGCTG TCCTCAAAGA CGTCTCCATG 
ATACAGAAAC TTCTGGCTGT GATGGCTCAG GATCAGGAGC AGATGCCGGA GGTCACCGAA 
TCAGGGATGA GCTTGAAGGA AGGCGAAGTC ACGGAGAATA TCGACCGCTC TTTTCTTGCC 
ACTTTAATGG GCCAGTCCCT TACGGATAAG CTGTACAACT CCTGGGTCCG GCTGCAGAGC 
CACGTCAATA TTGTCTTCGA TAGCGACATG GACAAGCTCA TGACTGAAAA GTACCCGGGA 
ATCCGCCAGG TCCTGGAGAA GAAAGAAGGT CTGTTTCGTA AGCACATGAT GGGGAAGCGA 
GTGGATTATG CAGCTCGCTC TGTTATCTGC CCCGACATGT ACATCAACAC CAATGAAATT 
GGAATCCCTA TGGTGTTTGC CACTAAGCTG ACGTACCCCC AGCCGGTCAC TCCCTGGAAT 
GTGCAAGAGT TGAGACAGGC TGTAATGAAC GGACCCAACG TCCATCCAGG TGCCTCCATG 
GTGATCAGTG AGGATGGCTC TCGCACCATA TTGAACTCTT CCAATGTATC CCAGAGGGAA 
GCTGTAGCAA AGCAACTACT CACCCCATCC ACTGGCGCAC AGACTCTGGC TGCTAAAATT 
GTGTGTCGCC ATGTGAAAAA TGGGGATGTG CTTCTCCTAA ACCGGCAGCC CACCCTTCAC 
AGGCCCTCCA TCCAGGCCCA CAAAGCCCGT ATTCTCCCAG GGGAGAAGGT GCTCCGGCTG 
CACTACGCCA ACTGTAAGGC GTACAATGCT GACTTCGATG GGGATGAAAT GAACGCGCAT 
TTCCCCCAAA GCGAGCTGGG CCGGGCTGAA GCCTACAGCT TAGCATGTAC AGATGAGCAG 
TACTTGGTGC CTAAGGATGG TACACCCCTT GCCGGCCTAA TTCAAGATCA TATGGTGTCC 
GGCGCAAACA TGACCATCCG CGGCTGCTTT TTCACCCGGG AACAGTATAT GGAATTGGTT 
TATCAAGGGC TGACAGATAA GCGAGGGCGC GTAAAAGTCC TCCCACCAGC TATCCTGAAA 
CCCTACCCAC TTTGGACAGG AAAGCAGGTT GTATCTACGT TACTAATCAA CGTAATCCCT 
GAAAATCTGA TTCCCCTCAA CCTGACCGGA AAAGCCAAAA TCAGCGGGAA AGCTTGGGTG 
AAGGAGCAGC CTCGGCCTAT TCCGGGATTC TCGCCGGACT CCATGTGCGA GTCTCAGGTA 
ATCATTAGAG ACGGGGAATT GCTTTGTGGC GTCCTGGACA AAGCCCACTA TGGGAGCTCT 
TCCTTCGGCT TGGTGCACTG CTGCTATGAG CTGTACGGAG GCGAGACCAG CGGCAAACTG 
CTGACTTGCT TGGCGAGGCT CTTCACTGCC TACCTCCAGC TCTACAGAGG GTTCACTCTG 
GGAGTTGAAG ACATTCTTGT GCTACCCTAT GCAGATGCCC AGAGACTGAA TATTGTACAG 
CAATCATTGC ATTGTGGTGG GAAGGCTATC AGAGCGGCTT TCAACTTGTC GGAAGCGGCG 
TCAGACGAAG AAGCGCACGG GAAGTGGCAG GATGCCCATC TCAGCAAAGA CCAGAGAGAT 
TTCAACATGG TGGATCTGAA ATTCAAGGAG GAAGTGAACC AGTTCAACAA CGAAATCAAT 
AAGGTGTGCA TGCCTTTCGG CCTCCATCGA CCCTTCCCAG AGAACAACCT GCAGCTGATG 
GTTCAGTCAG GAGCCAAAGG ATCCACTGTA AATACCATGC AGATTTCCTG TTTATTGGGC 
CAGATTGAGC TGGAGGGGCG GAGGCCCCCA CTGATGCCTT CTGGGAGGTC ATTACCTTGT 
TTCCAGCCAT ATGATTTTAG CCCACGATCT GGAGGATTTG TTACTGGCCG ATTTCTTACT 
GGCATCAAAC CTCCTGAGTT CTTCTTTCAT TGCATGGCAG GGAGGGAAGG ATTGGTGGAC 
ACGGCTGTGA AAACTAGTCG CTCGGGATAT CTGCAGAGGT GCATTATTAA ACATCTGGAG 
GGTCTTGTGG TTCAGTATGA CCTGACGGTG CGAGACAGCG ATGGCAGTGT CATTCAGTTC 
CTGTACGGAG AGGACGGCCT CGATGTGCCC AAGACACAGT TTCTCCAGCC CAAGCAGTTT 
CCATTCATAG CAGAGAACTA CGAGGTTATC AAGCAGTCCA AGCACTTGGA TGAAGTTCTA 
TCCAGGGTGA ACACAGAGCA AGCAGAGCAG CACAATCATG CCATCTATAG GTGGAAAGCA 
AGGCACCCCA ACCTCTCCCA GCGAATCAGC CCCTTCCTGC TCTACTCTCA GAAGCAAATG 
GCAGCTGCAA AAGCATCGTG TCCTAATGAG CTGGATGAGA ATGGCAGGGA TCTCGCATCC 
AGACAGGTAT TGGAGAGCTG GTATGCGGCG AATGAGAAGA CACGTAGCAA ATACGTGAGA 
AGGTCGTCTA CTCTCCCTGA TCCCTGCCTG GCAGTGTGGC GCCCAGATCT CTACTTGGGA 
TCCGTATCTG AGAACTTCCA GAGCAAAATG GCGCAGTATG GCTGTCTGTG GGAGGAGCAG 
AGGGACTCCA GCCATGGGAT GTCCTCGCTG ACATTTGACA GCTTAAAAAC CTTGCTGCAC 
CTAAAGTGGC AGAGAGCGCT GTGTGACCCC GGGGATGCGG TGGGGCTTCT GGCAGCTCAG 
AGCATCGGAG AGCCCTCCAC ACAGATGACG CTGAACACTT TCCACTTTGC TGGCAGAGGA 
GAAATGAACG TCACTCTGGG AATTCCTAGG CTTCGGGAGA TCCTGATGGT TGCAAGCGCC 
AACATAAAGA CGCCCATGAT GAGCGTGCCG GTTCTGAACA CTAAGAAAGC TGTGAAGAAG 
GTGAAGCAAC TGAAAAAGAA GCTAACGCGA GTGTGCCTGG CAGAGGTGAT ACACAAACTG 
GACGTGGAGG AGACGTTCCG TATCGAGGAG AAGTCACAGA AGTACCAAGT GTACAAGATC 
CGCTTCCATT TCCTACCCCA CGAGTACTAT CGGCAGGAGA AGCTCCTGCG GCCCGAGGGG 
GTCCTGCGCT TCATGGAAAC AAGGTTCTTC AAAATCATGT TTGATGCCAT AAAAAGGAAA 
AGCTCCAAGC TTGCTTCTTT CCGCGAAGTG GGCACCCGAA GGGCCACTCA GAAGGACCAT 
GGGGATGGGG AAGACCAAGG AAACCAGGAA AGCCAGCCCG TGGAGAATGA AGAAGAGCAA 
GTCAATGCGG ATGATGATGA TGATGATGCA GAGGAAGGTG ATGCTGATGC CACTGATAAA 
AAAAGAAGAA AAAAACAAGA GGAGGAGGTG GACTATGAGA GTGAGGAAGA GGAGGCTGAA 
GAAGACAATG AGGAGGAGGT GGGGTCTCCC GAGGCAGGAG AGGAGACATC TGCTGATGTG 
AAAGAGAACA CTGGGGATAT TGTGGATGAA ATAGAGGAGA ACTCCCACTC CCCCGTACCT 
GCCAGGAGAC CTCAAAGTGG ACTTGCCAAG GAGGTTGTGG CCGAAAACAG AATAAACGAT 
GTACTTAGCC TCAATTCCGC TATAGAAGAT TACTGCTATG ATACAGAGAA CAGCTTGTGG 
TGTGAGATCA CAATCAAACT GCAGCTGATG AAGATCCACT TCGATATCTG TTCCCTCGTG 
TCCTCCCTCG CCCACAGTGC TATAGTCCAT GAGACCAGCG GCATCACCCG GTGCCTCTTG 
AATGATACGG CCAACAAGCA AGGGGACAAA GAGCTGATTC TCAACACAGA GGGGATCAAT 
CTGTATGAAC TTTTCAAGTA TTCCAATATC CTGGAGCTGA GGAGGCTTTA TTCCAATGAT 
ATACACACCA TGGCAAAAAC CTTTGGCATT GAGGCTGCTT TACGGGTGGT AGAGAGGGAG 
ATTAAAGACG TGTTTGCGGT TTATGGTATC GAAGTGGATC CCCGGCACCT CTCGCTGGTT 
GCGGATTACA TGTGCTTCGA GGGTACCTAC AAGCCTCTCA ATCGGATCGG GATGCAGTCT 
AGCTCCTCGC CCTTCCAGCA GATGACATTT GAAACTAGCT ATAAATTTCT AAAGGATGCC 
ACCATGAATG GCGCTCGGGA TGACCTGCAG TCTCCGTCGG CGTGCTTAGT GGTTGGAAAG 
GTTGTAAAAG GAGGCACTGG TTTATTCGAC CTTAAGCAGC CCATGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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polr1a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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