dennd5a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dennd5a
Entrez Gene ID	100158564
Full Name	DENN/MADD domain containing 5A
General protein information	Preferred Names DENN/MADD domain containing 5A Names DENN domain-containing protein 5A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012960650.2	XP_012816104.2	DENN domain-containing protein 5A isoform X1
XM_018090115.1	XP_017945604.1	DENN domain-containing protein 5A isoform X3
NM_001127996.1	NP_001121468.1	DENN domain-containing protein 5A
XM_018090113.1	XP_017945602.1	DENN domain-containing protein 5A isoform X1
XM_018090114.1	XP_017945603.1	DENN domain-containing protein 5A isoform X2
XM_018090117.1	XP_017945606.1	DENN domain-containing protein 5A isoform X4
XM_031899879.1	XP_031755739.1	DENN domain-containing protein 5A isoform X1
XM_031899880.1	XP_031755740.1	DENN domain-containing protein 5A isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DENND5A	XP_508914.2
Bos taurus (cattle)	DENND5A	XP_002693152.1
Danio rerio (zebrafish)	dennd5a	XP_001340162.4
Rattus norvegicus (Norway rat)	Dennd5a	NP_001101016.2
Caenorhabditis elegans (roundworm)	C52E12.4	NP_495437.2
Xenopus tropicalis (tropical clawed frog)	dennd5a	NP_001121468.1
Homo sapiens (human)	DENND5A	NP_056028.2
	DENND5A	XP_002799687.1
Gallus gallus (chicken)	DENND5A	XP_004941436.1
Canis lupus familiaris (dog)	DENND5A	XP_534048.3
Mus musculus (house mouse)	Dennd5a	NP_067469.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dennd5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dennd5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa41184	XM_012960650.2 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa41184	XM_018090113.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa41185	XM_018090114.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa41186	XM_018090115.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa41187	XM_018090117.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa55809	XM_031899879.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa55810	XM_031899880.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa03116	NM_001127996.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dennd5a ( XM_012960650.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816104.2 Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa41184
Clone ID Related Accession (Same CDS sequence)	XM_012960650.2 , XM_018090113.1
Accession Version	XM_012960650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTATGCA TGCCTAAAGG ATTAGCATTT AAGACTCAGG CCGACCCCAA GGAGCCCCAG 
TACCACGCGT TCATCATCAC GAGAGAAGAC GGCTCTAGAA CATTTGGGTT CTCCCTCACC 
TTCTTTGAAG AAGTCACCAG CAAGCAGATA TGCAGCGCCA TGCAGACCCT GTACCACATG 
CATAACGCCG AGTATGACAT CATCCACACG TCGGCGAGCA ACGGCAGAGA CTGCCCCGGA 
GAGTGCACCG ACAGCTCCAT CACCAAGCTG CAGCGCTTCA ACTCTTATGA CATAACACGA 
GACACCCTGT ATGTGTCCAA GTGCATCTGT CTCATCGCCC CCATGTCTTT CATGACCGCC 
TGTAAGAAAG TCCTGGAGCA GCTCCACCAG GCTGTGACTT CCCCACAGCC GCCGCCATTA 
CCCTTAGAAA GTTACATTTA TAACATCCTC TATGAGGTGC CTCTGCCCCC GGCTGGGAGG 
TCCCTGAAGT TTAATGGGGT GTATGGACCA ATCGTGTGTC AGAGGCCGAG CACCAATGAG 
CTGCCCTTGT TTGACTTTCC TGCCCAGGAA GTGTTTGAGT TGCTTGGGGT GGAAAACGTG 
GTGCAGCTGT TTACGTGCGC CCTGCTGGAA TTCCAGATAT TGCTCTACTC CCAACATTAC 
CAGAGGTTAA TGACGGTGGC AGAGACCATC ACAGGCCTGA TGTTCCCATT TCAGTGGCAG 
CATGTCTACG TCCCCATCTT GCCAGCCTCC CTCCTCCATT TCCTGGACGC GCCGGTTCCG 
TACCTAATGG GATTGCACTC CAATGGGCTG GATGACAGAT CTAAGCTGGA GCTGCCACAA 
GAGGCCAACC TGTGCTTCGT GGACATAGAC AACCACTTTA TAGAGCTGCC CGAGGACCTG 
CCGCAGTTCC CCAACAAGCT GGAATTCATA CAGGAGGTAT CGGAGATCCT TTTGGCCTTC 
GGCATCCCCC CCGAGGGGAA CCTGCACTGC AGCGAGAGCA TCTCCAAGCT GAGGAACCTC 
AAGGCAGCCG ACCTGGTGTC CGACAAGAGG AACGGAAACC TGGCCGGCTC CCCATTAAAC 
TCCTATGAAC TTTTAAAAGA GAACGAGACT ATTGCCCGGC TTCAGGCGCT GGTGAAGAGG 
ACGGGGGTCA GTCTGGAAAA ACAGGTGGAA GTGCGGGAGG AGTCGAGCAG CAAAAAGGAC 
GGCAAAGCTC ACTGTGACGA GGAGGAGCTC CGAATGCACC AACTCAACAT CCAGGTCCGG 
GAAGTCTTTG CCAACCGCTT CACTCAGATG TTTGCCGATT ATGAGGTGTT TGTGATCCAG 
CCCAGCCAGG ACAAGGAATC CTGGTTCACT AACAGGGAGC AAATGCAGAA TTTTGATAAA 
GCCTCGTTCC TATCAGACCA GCCGGAGCCC TACCTCCCCT TCCTCTCCCG GTTCCTCGAG 
ACACAGATGT TTGCTTCCTT CATCGACAAT AAAATCATGT GCCACGATGA CGATGACAAG 
GATCCAGTTT TACGGGTATT TGATTCTCGG GTGGATAAAA TTCGCCAGCT AAACGTCCGG 
ACCCCCACTC TCCGCACATC CATGTACCAG AAGTGCACCG CCATCGAGGA GGCCGTAAAA 
GCCATAGAGC AAAGACTGTC CAAAATAGAC CACACGGCGA TCCACCCCCA TCTGCTGGAT 
ATGAAGATAG GGCAGGGAAA GTACGAGCCT GGATTCTTCC CCAAACTGCA GTCGGATGTT 
CTTTCCACGG GACCTGCCAC AAATAAATGG ACCAAGAGGA ACGCGCCAGC ACAGTGGAGG 
AGGAAGGACA GACAGAAGCA GCACTCAGAG CACCTTAGGC TGGACAATGA CCAGAGAGAG 
AAATACATTC AAGAAGCAAG AAATATGAGC AGCGGGATCC GGCAACCCAA ACTGTCCAAC 
CTGTCACCCT CTGTAATTGC GCAGACCAAC TGGAAGTTTG TGGAGGGGCT GCTGAAGGAA 
TGTCGGAACA AGACCAAGAG AATGCTGGTG GAGAAGATGG GAAGGGAAGC GGTAGAGCTG 
GGCCACGGAG AAGTGAGCAT TACAGGGGTA GAAGAGAATG CTCTGATCGC CAGTCTGTGT 
GACCTGCTGG AGAGAATTTG GAGCCACGGT CTGCAAGTGA AGCAGGGGAA GTCAGCCTTG 
TGGTCACACT TACTGCATTA CCAGGACAGC AGGAGGAGAG CGACCTGGGG CAGTCTTGGC 
GGAACAGATT TGGTACTGGA CTGTGAGAGA AGGAAGTCTG ACCCCTTTCT GGTCATGTAC 
CCCCTAAAGA TCTCACTTGT TCAGGATATG AGGCATATTC AGAACATTGG AGAAATCAAG 
ACAGATGTAG GGAAGGCGCG AGCCTGGGTC AGGCTGTCTA TGGAAAAGAA ACTTCTGTCC 
CGGCACCTGA AGCAGCTGCT GTTGGATCAT GATCTCAGCA AGAAGCTGTA CAAGCGCTAC 
GCCTTCCTGC GCTGCGACGA TGAGAAGGAA CAGTTCCTCT ATCATTTGCT CTCCTTCAAT 
GCTGTGGATT ATTTCTGCTT TACTAACGTG TTTACAACTA TCATGATCCC CTACCACATC 
CTGATTGTCC CTAGCAAGAA GCTCGGCGGC TCCATGTTCA CAGCCAACCC CTGGATCTGT 
ATATCTGGGG AGCTGGGAGA GACTGGGGTA CTGCAGGTTC CCAGGAATAT TCTAGAGATG 
ACGTATGAGT GCCAGAACCT CGGGAAGCTG ACGACTGTAC AGATTGGACA CGACAATTCT 
GGCCTGTATG CCAAGTGGCT GGTGGAATAT GTTATGGTCC GAAATGAAAT AACTGGACAC 
ACCTACAAGT TCCCTTGTGG GCGCTGGTTG GGAAAAGGAA TGGATGATGG CAGTCTGGAA 
CGTGTGCTGG TTGGAGACCT GCTTACATCC AGTACAGACA CTGAAGAGAA ACCATGCCGG 
ACCCCTCCCC TGCAGCACTC ACCCAGCATG ATCAGGAGGC TTGTGGCCAT ATCCCCCAAT 
AGCCGACCAA AATTAAATAC GGGTCAGATC CAGGAGGCTA TTGGAGAAGC AGTGAATGGA 
ATTGTGAAAC ATTTTCATAA ACCAGAGAAA GAGAGAGGCA GTTTAACGTT ATTACTGTGC 
GGAGAGAACG GACTGGTGTC TGCCCTTGAA CAAGTTTTCC AGCATGGAAT CCGATCCCCT 
CGGCTTTTCA AAAGCATCTT CATCTGGGAC TTCTTAGAAA AAGCACAAGC TTATTACGAA 
TCTCTGGAAG TGAATGAATT GGTGCCAGAA GAGGACTGGC AGAAACGGGC GCGGAATTTC 
TCCCGCTTTA TTAACGCCAT TAACAACAAC CCTAGAAACA TTGGGAAAGA CGGCAAGTTT 
CAGATGTTTG TTTGCTTAGG AGCCCGGGAC CACCATTTGC ACCACTGGAT TGCACTGCTG 
GCCGACTGCC CCATTATAGC ACAAATGTAC GAGGAGACGG CTCTAATCAA GGACCACACG 
TTGGTGAACT CTCTAATCCG AGTGTTACAG ACACTACAAG AGTTTAACAT CACCTTAGAA 
AGCTCCCTCA TTAAAGGAAT CAATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816104.2
CDS	755..4324
Translation	MLCMPKGLAFKTQADPKEPQYHAFIITREDGSRTFGFSLTFFEEVTSKQICSAMQTLYHMHNAEYDIIHTSASNGRDCPGECTDSSITKLQRFNSYDITRDTLYVSKCICLIAPMSFMTACKKVLEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPAGRSLKFNGVYGPIVCQRPSTNELPLFDFPAQEVFELLGVENVVQLFTCALLEFQILLYSQHYQRLMTVAETITGLMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEVSEILLAFGIPPEGNLHCSESISKLRNLKAADLVSDKRNGNLAGSPLNSYELLKENETIARLQALVKRTGVSLEKQVEVREESSSKKDGKAHCDEEELRMHQLNIQVREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDNKIMCHDDDDKDPVLRVFDSRVDKIRQLNVRTPTLRTSMYQKCTAIEEAVKAIEQRLSKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQSDVLSTGPATNKWTKRNAPAQWRRKDRQKQHSEHLRLDNDQREKYIQEARNMSSGIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENALIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQDSRRRATWGSLGGTDLVLDCERRKSDPFLVMYPLKISLVQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLLDHDLSKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETGVLQVPRNILEMTYECQNLGKLTTVQIGHDNSGLYAKWLVEYVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGDLLTSSTDTEEKPCRTPPLQHSPSMIRRLVAISPNSRPKLNTGQIQEAIGEAVNGIVKHFHKPEKERGSLTLLLCGENGLVSALEQVFQHGIRSPRLFKSIFIWDFLEKAQAYYESLEVNELVPEEDWQKRARNFSRFINAINNNPRNIGKDGKFQMFVCLGARDHHLHHWIALLADCPIIAQMYEETALIKDHTLVNSLIRVLQTLQEFNITLESSLIKGINI

Target ORF information:

RefSeq Version	XM_012960650.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960650.2

ORF Insert Sequence:

ATGCTATGCA TGCCTAAAGG ATTAGCATTT AAGACTCAGG CCGACCCCAA GGAGCCCCAG 
TACCACGCGT TCATCATCAC GAGAGAAGAC GGCTCTAGAA CATTTGGGTT CTCCCTCACC 
TTCTTTGAAG AAGTCACCAG CAAGCAGATA TGCAGCGCCA TGCAGACCCT GTACCACATG 
CATAACGCCG AGTATGACAT CATCCACACG TCGGCGAGCA ACGGCAGAGA CTGCCCCGGA 
GAGTGCACCG ACAGCTCCAT CACCAAGCTG CAGCGCTTCA ACTCTTATGA CATAACACGA 
GACACCCTGT ATGTGTCCAA GTGCATCTGT CTCATCGCCC CCATGTCTTT CATGACCGCC 
TGTAAGAAAG TCCTGGAGCA GCTCCACCAG GCTGTGACTT CCCCACAGCC GCCGCCATTA 
CCCTTAGAAA GTTACATTTA TAACATCCTC TATGAGGTGC CTCTGCCCCC GGCTGGGAGG 
TCCCTGAAGT TTAATGGGGT GTATGGACCA ATCGTGTGTC AGAGGCCGAG CACCAATGAG 
CTGCCCTTGT TTGACTTTCC TGCCCAGGAA GTGTTTGAGT TGCTTGGGGT GGAAAACGTG 
GTGCAGCTGT TTACGTGCGC CCTGCTGGAA TTCCAGATAT TGCTCTACTC CCAACATTAC 
CAGAGGTTAA TGACGGTGGC AGAGACCATC ACAGGCCTGA TGTTCCCATT TCAGTGGCAG 
CATGTCTACG TCCCCATCTT GCCAGCCTCC CTCCTCCATT TCCTGGACGC GCCGGTTCCG 
TACCTAATGG GATTGCACTC CAATGGGCTG GATGACAGAT CTAAGCTGGA GCTGCCACAA 
GAGGCCAACC TGTGCTTCGT GGACATAGAC AACCACTTTA TAGAGCTGCC CGAGGACCTG 
CCGCAGTTCC CCAACAAGCT GGAATTCATA CAGGAGGTAT CGGAGATCCT TTTGGCCTTC 
GGCATCCCCC CCGAGGGGAA CCTGCACTGC AGCGAGAGCA TCTCCAAGCT GAGGAACCTC 
AAGGCAGCCG ACCTGGTGTC CGACAAGAGG AACGGAAACC TGGCCGGCTC CCCATTAAAC 
TCCTATGAAC TTTTAAAAGA GAACGAGACT ATTGCCCGGC TTCAGGCGCT GGTGAAGAGG 
ACGGGGGTCA GTCTGGAAAA ACAGGTGGAA GTGCGGGAGG AGTCGAGCAG CAAAAAGGAC 
GGCAAAGCTC ACTGTGACGA GGAGGAGCTC CGAATGCACC AACTCAACAT CCAGGTCCGG 
GAAGTCTTTG CCAACCGCTT CACTCAGATG TTTGCCGATT ATGAGGTGTT TGTGATCCAG 
CCCAGCCAGG ACAAGGAATC CTGGTTCACT AACAGGGAGC AAATGCAGAA TTTTGATAAA 
GCCTCGTTCC TATCAGACCA GCCGGAGCCC TACCTCCCCT TCCTCTCCCG GTTCCTCGAG 
ACACAGATGT TTGCTTCCTT CATCGACAAT AAAATCATGT GCCACGATGA CGATGACAAG 
GATCCAGTTT TACGGGTATT TGATTCTCGG GTGGATAAAA TTCGCCAGCT AAACGTCCGG 
ACCCCCACTC TCCGCACATC CATGTACCAG AAGTGCACCG CCATCGAGGA GGCCGTAAAA 
GCCATAGAGC AAAGACTGTC CAAAATAGAC CACACGGCGA TCCACCCCCA TCTGCTGGAT 
ATGAAGATAG GGCAGGGAAA GTACGAGCCT GGATTCTTCC CCAAACTGCA GTCGGATGTT 
CTTTCCACGG GACCTGCCAC AAATAAATGG ACCAAGAGGA ACGCGCCAGC ACAGTGGAGG 
AGGAAGGACA GACAGAAGCA GCACTCAGAG CACCTTAGGC TGGACAATGA CCAGAGAGAG 
AAATACATTC AAGAAGCAAG AAATATGAGC AGCGGGATCC GGCAACCCAA ACTGTCCAAC 
CTGTCACCCT CTGTAATTGC GCAGACCAAC TGGAAGTTTG TGGAGGGGCT GCTGAAGGAA 
TGTCGGAACA AGACCAAGAG AATGCTGGTG GAGAAGATGG GAAGGGAAGC GGTAGAGCTG 
GGCCACGGAG AAGTGAGCAT TACAGGGGTA GAAGAGAATG CTCTGATCGC CAGTCTGTGT 
GACCTGCTGG AGAGAATTTG GAGCCACGGT CTGCAAGTGA AGCAGGGGAA GTCAGCCTTG 
TGGTCACACT TACTGCATTA CCAGGACAGC AGGAGGAGAG CGACCTGGGG CAGTCTTGGC 
GGAACAGATT TGGTACTGGA CTGTGAGAGA AGGAAGTCTG ACCCCTTTCT GGTCATGTAC 
CCCCTAAAGA TCTCACTTGT TCAGGATATG AGGCATATTC AGAACATTGG AGAAATCAAG 
ACAGATGTAG GGAAGGCGCG AGCCTGGGTC AGGCTGTCTA TGGAAAAGAA ACTTCTGTCC 
CGGCACCTGA AGCAGCTGCT GTTGGATCAT GATCTCAGCA AGAAGCTGTA CAAGCGCTAC 
GCCTTCCTGC GCTGCGACGA TGAGAAGGAA CAGTTCCTCT ATCATTTGCT CTCCTTCAAT 
GCTGTGGATT ATTTCTGCTT TACTAACGTG TTTACAACTA TCATGATCCC CTACCACATC 
CTGATTGTCC CTAGCAAGAA GCTCGGCGGC TCCATGTTCA CAGCCAACCC CTGGATCTGT 
ATATCTGGGG AGCTGGGAGA GACTGGGGTA CTGCAGGTTC CCAGGAATAT TCTAGAGATG 
ACGTATGAGT GCCAGAACCT CGGGAAGCTG ACGACTGTAC AGATTGGACA CGACAATTCT 
GGCCTGTATG CCAAGTGGCT GGTGGAATAT GTTATGGTCC GAAATGAAAT AACTGGACAC 
ACCTACAAGT TCCCTTGTGG GCGCTGGTTG GGAAAAGGAA TGGATGATGG CAGTCTGGAA 
CGTGTGCTGG TTGGAGACCT GCTTACATCC AGTACAGACA CTGAAGAGAA ACCATGCCGG 
ACCCCTCCCC TGCAGCACTC ACCCAGCATG ATCAGGAGGC TTGTGGCCAT ATCCCCCAAT 
AGCCGACCAA AATTAAATAC GGGTCAGATC CAGGAGGCTA TTGGAGAAGC AGTGAATGGA 
ATTGTGAAAC ATTTTCATAA ACCAGAGAAA GAGAGAGGCA GTTTAACGTT ATTACTGTGC 
GGAGAGAACG GACTGGTGTC TGCCCTTGAA CAAGTTTTCC AGCATGGAAT CCGATCCCCT 
CGGCTTTTCA AAAGCATCTT CATCTGGGAC TTCTTAGAAA AAGCACAAGC TTATTACGAA 
TCTCTGGAAG TGAATGAATT GGTGCCAGAA GAGGACTGGC AGAAACGGGC GCGGAATTTC 
TCCCGCTTTA TTAACGCCAT TAACAACAAC CCTAGAAACA TTGGGAAAGA CGGCAAGTTT 
CAGATGTTTG TTTGCTTAGG AGCCCGGGAC CACCATTTGC ACCACTGGAT TGCACTGCTG 
GCCGACTGCC CCATTATAGC ACAAATGTAC GAGGAGACGG CTCTAATCAA GGACCACACG 
TTGGTGAACT CTCTAATCCG AGTGTTACAG ACACTACAAG AGTTTAACAT CACCTTAGAA 
AGCTCCCTCA TTAAAGGAAT CAATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_018090113.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945602.1 Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa41184
Clone ID Related Accession (Same CDS sequence)	XM_012960650.2 , XM_018090113.1
Accession Version	XM_018090113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTATGCA TGCCTAAAGG ATTAGCATTT AAGACTCAGG CCGACCCCAA GGAGCCCCAG 
TACCACGCGT TCATCATCAC GAGAGAAGAC GGCTCTAGAA CATTTGGGTT CTCCCTCACC 
TTCTTTGAAG AAGTCACCAG CAAGCAGATA TGCAGCGCCA TGCAGACCCT GTACCACATG 
CATAACGCCG AGTATGACAT CATCCACACG TCGGCGAGCA ACGGCAGAGA CTGCCCCGGA 
GAGTGCACCG ACAGCTCCAT CACCAAGCTG CAGCGCTTCA ACTCTTATGA CATAACACGA 
GACACCCTGT ATGTGTCCAA GTGCATCTGT CTCATCGCCC CCATGTCTTT CATGACCGCC 
TGTAAGAAAG TCCTGGAGCA GCTCCACCAG GCTGTGACTT CCCCACAGCC GCCGCCATTA 
CCCTTAGAAA GTTACATTTA TAACATCCTC TATGAGGTGC CTCTGCCCCC GGCTGGGAGG 
TCCCTGAAGT TTAATGGGGT GTATGGACCA ATCGTGTGTC AGAGGCCGAG CACCAATGAG 
CTGCCCTTGT TTGACTTTCC TGCCCAGGAA GTGTTTGAGT TGCTTGGGGT GGAAAACGTG 
GTGCAGCTGT TTACGTGCGC CCTGCTGGAA TTCCAGATAT TGCTCTACTC CCAACATTAC 
CAGAGGTTAA TGACGGTGGC AGAGACCATC ACAGGCCTGA TGTTCCCATT TCAGTGGCAG 
CATGTCTACG TCCCCATCTT GCCAGCCTCC CTCCTCCATT TCCTGGACGC GCCGGTTCCG 
TACCTAATGG GATTGCACTC CAATGGGCTG GATGACAGAT CTAAGCTGGA GCTGCCACAA 
GAGGCCAACC TGTGCTTCGT GGACATAGAC AACCACTTTA TAGAGCTGCC CGAGGACCTG 
CCGCAGTTCC CCAACAAGCT GGAATTCATA CAGGAGGTAT CGGAGATCCT TTTGGCCTTC 
GGCATCCCCC CCGAGGGGAA CCTGCACTGC AGCGAGAGCA TCTCCAAGCT GAGGAACCTC 
AAGGCAGCCG ACCTGGTGTC CGACAAGAGG AACGGAAACC TGGCCGGCTC CCCATTAAAC 
TCCTATGAAC TTTTAAAAGA GAACGAGACT ATTGCCCGGC TTCAGGCGCT GGTGAAGAGG 
ACGGGGGTCA GTCTGGAAAA ACAGGTGGAA GTGCGGGAGG AGTCGAGCAG CAAAAAGGAC 
GGCAAAGCTC ACTGTGACGA GGAGGAGCTC CGAATGCACC AACTCAACAT CCAGGTCCGG 
GAAGTCTTTG CCAACCGCTT CACTCAGATG TTTGCCGATT ATGAGGTGTT TGTGATCCAG 
CCCAGCCAGG ACAAGGAATC CTGGTTCACT AACAGGGAGC AAATGCAGAA TTTTGATAAA 
GCCTCGTTCC TATCAGACCA GCCGGAGCCC TACCTCCCCT TCCTCTCCCG GTTCCTCGAG 
ACACAGATGT TTGCTTCCTT CATCGACAAT AAAATCATGT GCCACGATGA CGATGACAAG 
GATCCAGTTT TACGGGTATT TGATTCTCGG GTGGATAAAA TTCGCCAGCT AAACGTCCGG 
ACCCCCACTC TCCGCACATC CATGTACCAG AAGTGCACCG CCATCGAGGA GGCCGTAAAA 
GCCATAGAGC AAAGACTGTC CAAAATAGAC CACACGGCGA TCCACCCCCA TCTGCTGGAT 
ATGAAGATAG GGCAGGGAAA GTACGAGCCT GGATTCTTCC CCAAACTGCA GTCGGATGTT 
CTTTCCACGG GACCTGCCAC AAATAAATGG ACCAAGAGGA ACGCGCCAGC ACAGTGGAGG 
AGGAAGGACA GACAGAAGCA GCACTCAGAG CACCTTAGGC TGGACAATGA CCAGAGAGAG 
AAATACATTC AAGAAGCAAG AAATATGAGC AGCGGGATCC GGCAACCCAA ACTGTCCAAC 
CTGTCACCCT CTGTAATTGC GCAGACCAAC TGGAAGTTTG TGGAGGGGCT GCTGAAGGAA 
TGTCGGAACA AGACCAAGAG AATGCTGGTG GAGAAGATGG GAAGGGAAGC GGTAGAGCTG 
GGCCACGGAG AAGTGAGCAT TACAGGGGTA GAAGAGAATG CTCTGATCGC CAGTCTGTGT 
GACCTGCTGG AGAGAATTTG GAGCCACGGT CTGCAAGTGA AGCAGGGGAA GTCAGCCTTG 
TGGTCACACT TACTGCATTA CCAGGACAGC AGGAGGAGAG CGACCTGGGG CAGTCTTGGC 
GGAACAGATT TGGTACTGGA CTGTGAGAGA AGGAAGTCTG ACCCCTTTCT GGTCATGTAC 
CCCCTAAAGA TCTCACTTGT TCAGGATATG AGGCATATTC AGAACATTGG AGAAATCAAG 
ACAGATGTAG GGAAGGCGCG AGCCTGGGTC AGGCTGTCTA TGGAAAAGAA ACTTCTGTCC 
CGGCACCTGA AGCAGCTGCT GTTGGATCAT GATCTCAGCA AGAAGCTGTA CAAGCGCTAC 
GCCTTCCTGC GCTGCGACGA TGAGAAGGAA CAGTTCCTCT ATCATTTGCT CTCCTTCAAT 
GCTGTGGATT ATTTCTGCTT TACTAACGTG TTTACAACTA TCATGATCCC CTACCACATC 
CTGATTGTCC CTAGCAAGAA GCTCGGCGGC TCCATGTTCA CAGCCAACCC CTGGATCTGT 
ATATCTGGGG AGCTGGGAGA GACTGGGGTA CTGCAGGTTC CCAGGAATAT TCTAGAGATG 
ACGTATGAGT GCCAGAACCT CGGGAAGCTG ACGACTGTAC AGATTGGACA CGACAATTCT 
GGCCTGTATG CCAAGTGGCT GGTGGAATAT GTTATGGTCC GAAATGAAAT AACTGGACAC 
ACCTACAAGT TCCCTTGTGG GCGCTGGTTG GGAAAAGGAA TGGATGATGG CAGTCTGGAA 
CGTGTGCTGG TTGGAGACCT GCTTACATCC AGTACAGACA CTGAAGAGAA ACCATGCCGG 
ACCCCTCCCC TGCAGCACTC ACCCAGCATG ATCAGGAGGC TTGTGGCCAT ATCCCCCAAT 
AGCCGACCAA AATTAAATAC GGGTCAGATC CAGGAGGCTA TTGGAGAAGC AGTGAATGGA 
ATTGTGAAAC ATTTTCATAA ACCAGAGAAA GAGAGAGGCA GTTTAACGTT ATTACTGTGC 
GGAGAGAACG GACTGGTGTC TGCCCTTGAA CAAGTTTTCC AGCATGGAAT CCGATCCCCT 
CGGCTTTTCA AAAGCATCTT CATCTGGGAC TTCTTAGAAA AAGCACAAGC TTATTACGAA 
TCTCTGGAAG TGAATGAATT GGTGCCAGAA GAGGACTGGC AGAAACGGGC GCGGAATTTC 
TCCCGCTTTA TTAACGCCAT TAACAACAAC CCTAGAAACA TTGGGAAAGA CGGCAAGTTT 
CAGATGTTTG TTTGCTTAGG AGCCCGGGAC CACCATTTGC ACCACTGGAT TGCACTGCTG 
GCCGACTGCC CCATTATAGC ACAAATGTAC GAGGAGACGG CTCTAATCAA GGACCACACG 
TTGGTGAACT CTCTAATCCG AGTGTTACAG ACACTACAAG AGTTTAACAT CACCTTAGAA 
AGCTCCCTCA TTAAAGGAAT CAATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945602.1
CDS	257..3826
Translation	MLCMPKGLAFKTQADPKEPQYHAFIITREDGSRTFGFSLTFFEEVTSKQICSAMQTLYHMHNAEYDIIHTSASNGRDCPGECTDSSITKLQRFNSYDITRDTLYVSKCICLIAPMSFMTACKKVLEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPAGRSLKFNGVYGPIVCQRPSTNELPLFDFPAQEVFELLGVENVVQLFTCALLEFQILLYSQHYQRLMTVAETITGLMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEVSEILLAFGIPPEGNLHCSESISKLRNLKAADLVSDKRNGNLAGSPLNSYELLKENETIARLQALVKRTGVSLEKQVEVREESSSKKDGKAHCDEEELRMHQLNIQVREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDNKIMCHDDDDKDPVLRVFDSRVDKIRQLNVRTPTLRTSMYQKCTAIEEAVKAIEQRLSKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQSDVLSTGPATNKWTKRNAPAQWRRKDRQKQHSEHLRLDNDQREKYIQEARNMSSGIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENALIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQDSRRRATWGSLGGTDLVLDCERRKSDPFLVMYPLKISLVQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLLDHDLSKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETGVLQVPRNILEMTYECQNLGKLTTVQIGHDNSGLYAKWLVEYVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGDLLTSSTDTEEKPCRTPPLQHSPSMIRRLVAISPNSRPKLNTGQIQEAIGEAVNGIVKHFHKPEKERGSLTLLLCGENGLVSALEQVFQHGIRSPRLFKSIFIWDFLEKAQAYYESLEVNELVPEEDWQKRARNFSRFINAINNNPRNIGKDGKFQMFVCLGARDHHLHHWIALLADCPIIAQMYEETALIKDHTLVNSLIRVLQTLQEFNITLESSLIKGINI

Target ORF information:

RefSeq Version	XM_018090113.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090113.1

ORF Insert Sequence:

ATGCTATGCA TGCCTAAAGG ATTAGCATTT AAGACTCAGG CCGACCCCAA GGAGCCCCAG 
TACCACGCGT TCATCATCAC GAGAGAAGAC GGCTCTAGAA CATTTGGGTT CTCCCTCACC 
TTCTTTGAAG AAGTCACCAG CAAGCAGATA TGCAGCGCCA TGCAGACCCT GTACCACATG 
CATAACGCCG AGTATGACAT CATCCACACG TCGGCGAGCA ACGGCAGAGA CTGCCCCGGA 
GAGTGCACCG ACAGCTCCAT CACCAAGCTG CAGCGCTTCA ACTCTTATGA CATAACACGA 
GACACCCTGT ATGTGTCCAA GTGCATCTGT CTCATCGCCC CCATGTCTTT CATGACCGCC 
TGTAAGAAAG TCCTGGAGCA GCTCCACCAG GCTGTGACTT CCCCACAGCC GCCGCCATTA 
CCCTTAGAAA GTTACATTTA TAACATCCTC TATGAGGTGC CTCTGCCCCC GGCTGGGAGG 
TCCCTGAAGT TTAATGGGGT GTATGGACCA ATCGTGTGTC AGAGGCCGAG CACCAATGAG 
CTGCCCTTGT TTGACTTTCC TGCCCAGGAA GTGTTTGAGT TGCTTGGGGT GGAAAACGTG 
GTGCAGCTGT TTACGTGCGC CCTGCTGGAA TTCCAGATAT TGCTCTACTC CCAACATTAC 
CAGAGGTTAA TGACGGTGGC AGAGACCATC ACAGGCCTGA TGTTCCCATT TCAGTGGCAG 
CATGTCTACG TCCCCATCTT GCCAGCCTCC CTCCTCCATT TCCTGGACGC GCCGGTTCCG 
TACCTAATGG GATTGCACTC CAATGGGCTG GATGACAGAT CTAAGCTGGA GCTGCCACAA 
GAGGCCAACC TGTGCTTCGT GGACATAGAC AACCACTTTA TAGAGCTGCC CGAGGACCTG 
CCGCAGTTCC CCAACAAGCT GGAATTCATA CAGGAGGTAT CGGAGATCCT TTTGGCCTTC 
GGCATCCCCC CCGAGGGGAA CCTGCACTGC AGCGAGAGCA TCTCCAAGCT GAGGAACCTC 
AAGGCAGCCG ACCTGGTGTC CGACAAGAGG AACGGAAACC TGGCCGGCTC CCCATTAAAC 
TCCTATGAAC TTTTAAAAGA GAACGAGACT ATTGCCCGGC TTCAGGCGCT GGTGAAGAGG 
ACGGGGGTCA GTCTGGAAAA ACAGGTGGAA GTGCGGGAGG AGTCGAGCAG CAAAAAGGAC 
GGCAAAGCTC ACTGTGACGA GGAGGAGCTC CGAATGCACC AACTCAACAT CCAGGTCCGG 
GAAGTCTTTG CCAACCGCTT CACTCAGATG TTTGCCGATT ATGAGGTGTT TGTGATCCAG 
CCCAGCCAGG ACAAGGAATC CTGGTTCACT AACAGGGAGC AAATGCAGAA TTTTGATAAA 
GCCTCGTTCC TATCAGACCA GCCGGAGCCC TACCTCCCCT TCCTCTCCCG GTTCCTCGAG 
ACACAGATGT TTGCTTCCTT CATCGACAAT AAAATCATGT GCCACGATGA CGATGACAAG 
GATCCAGTTT TACGGGTATT TGATTCTCGG GTGGATAAAA TTCGCCAGCT AAACGTCCGG 
ACCCCCACTC TCCGCACATC CATGTACCAG AAGTGCACCG CCATCGAGGA GGCCGTAAAA 
GCCATAGAGC AAAGACTGTC CAAAATAGAC CACACGGCGA TCCACCCCCA TCTGCTGGAT 
ATGAAGATAG GGCAGGGAAA GTACGAGCCT GGATTCTTCC CCAAACTGCA GTCGGATGTT 
CTTTCCACGG GACCTGCCAC AAATAAATGG ACCAAGAGGA ACGCGCCAGC ACAGTGGAGG 
AGGAAGGACA GACAGAAGCA GCACTCAGAG CACCTTAGGC TGGACAATGA CCAGAGAGAG 
AAATACATTC AAGAAGCAAG AAATATGAGC AGCGGGATCC GGCAACCCAA ACTGTCCAAC 
CTGTCACCCT CTGTAATTGC GCAGACCAAC TGGAAGTTTG TGGAGGGGCT GCTGAAGGAA 
TGTCGGAACA AGACCAAGAG AATGCTGGTG GAGAAGATGG GAAGGGAAGC GGTAGAGCTG 
GGCCACGGAG AAGTGAGCAT TACAGGGGTA GAAGAGAATG CTCTGATCGC CAGTCTGTGT 
GACCTGCTGG AGAGAATTTG GAGCCACGGT CTGCAAGTGA AGCAGGGGAA GTCAGCCTTG 
TGGTCACACT TACTGCATTA CCAGGACAGC AGGAGGAGAG CGACCTGGGG CAGTCTTGGC 
GGAACAGATT TGGTACTGGA CTGTGAGAGA AGGAAGTCTG ACCCCTTTCT GGTCATGTAC 
CCCCTAAAGA TCTCACTTGT TCAGGATATG AGGCATATTC AGAACATTGG AGAAATCAAG 
ACAGATGTAG GGAAGGCGCG AGCCTGGGTC AGGCTGTCTA TGGAAAAGAA ACTTCTGTCC 
CGGCACCTGA AGCAGCTGCT GTTGGATCAT GATCTCAGCA AGAAGCTGTA CAAGCGCTAC 
GCCTTCCTGC GCTGCGACGA TGAGAAGGAA CAGTTCCTCT ATCATTTGCT CTCCTTCAAT 
GCTGTGGATT ATTTCTGCTT TACTAACGTG TTTACAACTA TCATGATCCC CTACCACATC 
CTGATTGTCC CTAGCAAGAA GCTCGGCGGC TCCATGTTCA CAGCCAACCC CTGGATCTGT 
ATATCTGGGG AGCTGGGAGA GACTGGGGTA CTGCAGGTTC CCAGGAATAT TCTAGAGATG 
ACGTATGAGT GCCAGAACCT CGGGAAGCTG ACGACTGTAC AGATTGGACA CGACAATTCT 
GGCCTGTATG CCAAGTGGCT GGTGGAATAT GTTATGGTCC GAAATGAAAT AACTGGACAC 
ACCTACAAGT TCCCTTGTGG GCGCTGGTTG GGAAAAGGAA TGGATGATGG CAGTCTGGAA 
CGTGTGCTGG TTGGAGACCT GCTTACATCC AGTACAGACA CTGAAGAGAA ACCATGCCGG 
ACCCCTCCCC TGCAGCACTC ACCCAGCATG ATCAGGAGGC TTGTGGCCAT ATCCCCCAAT 
AGCCGACCAA AATTAAATAC GGGTCAGATC CAGGAGGCTA TTGGAGAAGC AGTGAATGGA 
ATTGTGAAAC ATTTTCATAA ACCAGAGAAA GAGAGAGGCA GTTTAACGTT ATTACTGTGC 
GGAGAGAACG GACTGGTGTC TGCCCTTGAA CAAGTTTTCC AGCATGGAAT CCGATCCCCT 
CGGCTTTTCA AAAGCATCTT CATCTGGGAC TTCTTAGAAA AAGCACAAGC TTATTACGAA 
TCTCTGGAAG TGAATGAATT GGTGCCAGAA GAGGACTGGC AGAAACGGGC GCGGAATTTC 
TCCCGCTTTA TTAACGCCAT TAACAACAAC CCTAGAAACA TTGGGAAAGA CGGCAAGTTT 
CAGATGTTTG TTTGCTTAGG AGCCCGGGAC CACCATTTGC ACCACTGGAT TGCACTGCTG 
GCCGACTGCC CCATTATAGC ACAAATGTAC GAGGAGACGG CTCTAATCAA GGACCACACG 
TTGGTGAACT CTCTAATCCG AGTGTTACAG ACACTACAAG AGTTTAACAT CACCTTAGAA 
AGCTCCCTCA TTAAAGGAAT CAATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_018090114.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945603.1 Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X3, mRNA.

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CloneID	OXa41185
Clone ID Related Accession (Same CDS sequence)	XM_018090114.1
Accession Version	XM_018090114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTATGCA TGCCTAAAGG ATTAGCATTT AAGACTCAGG CCGACCCCAA GGAGCCCCAG 
TACCACGCGT TCATCATCAC GAGAGAAGAC GGCTCTAGAA CATTTGGGTT CTCCCTCACC 
TTCTTTGAAG AAGTCACCAG CAAGCAGATA TGCAGCGCCA TGCAGACCCT GTACCACATG 
CATAACGCCG AGTATGACAT CATCCACACG TCGGCGAGCA ACGGCAGAGA CTGCCCCGGA 
GAGTGCACCG ACAGCTCCAT CACCAAGCTG CAGCGCTTCA ACTCTTATGA CATAACACGA 
GACACCCTGT ATGTGTCCAA GTGCATCTGT CTCATCGCCC CCATGTCTTT CATGACCGCC 
TGTAAGAAAG TCCTGGAGCA GCTCCACCAG GCTGTGACTT CCCCACAGCC GCCGCCATTA 
CCCTTAGAAA GTTACATTTA TAACATCCTC TATGAGGTGC CTCTGCCCCC GGCTGGGAGG 
TCCCTGAAGT TTAATGGGGT GTATGGACCA ATCGTGTGTC AGAGGCCGAG CACCAATGAG 
CTGCCCTTGT TTGACTTTCC TGCCCAGGAA GTGTTTGAGT TGCTTGGGGT GGAAAACGTG 
GTGCAGCTGT TTACGTGCGC CCTGCTGGAA TTCCAGATAT TGCTCTACTC CCAACATTAC 
CAGAGGTTAA TGACGGTGGC AGAGACCATC ACAGGCCTGA TGTTCCCATT TCAGTGGCAG 
CATGTCTACG TCCCCATCTT GCCAGCCTCC CTCCTCCATT TCCTGGACGC GCCGGTTCCG 
TACCTAATGG GATTGCACTC CAATGGGCTG GATGACAGAT CTAAGCTGGA GCTGCCACAA 
GAGGCCAACC TGTGCTTCGT GGACATAGAC AACCACTTTA TAGAGCTGCC CGAGGACCTG 
CCGCAGTTCC CCAACAAGCT GGAATTCATA CAGGAGGTAT CGGAGATCCT TTTGGCCTTC 
GGCATCCCCC CCGAGGGGAA CCTGCACTGC AGCGAGAGCA TCTCCAAGCT GAGGAACCTC 
AAGGCAGCCG ACCTGGTGTC CGACAAGAGG AACGGAAACC TGGCCGGCTC CCCATTAAAC 
TCCTATGAAC TTTTAAAAGA GAACGAGACT ATTGCCCGGC TTCAGGCGCT GGTGAAGAGG 
ACGGGGGTCA GTCTGGAAAA ACAGGTGGAA GTGCGGGAGG AGTCGAGCAG CAAAAAGGAC 
GGCAAAGCTC ACTGTGACGA GGAGGAGCTC CGAATGCACC AACTCAACAT CCAGGTCCGG 
GAAGTCTTTG CCAACCGCTT CACTCAGATG TTTGCCGATT ATGAGGTGTT TGTGATCCAG 
CCCAGCCAGG ACAAGGAATC CTGGTTCACT AACAGGGAGC AAATGCAGAA TTTTGATAAA 
GCCTCGTTCC TATCAGACCA GCCGGAGCCC TACCTCCCCT TCCTCTCCCG GTTCCTCGAG 
ACACAGATGT TTGCTTCCTT CATCGACAAT AAAATCATGT GCCACGATGA CGATGACAAG 
GATCCAGTTT TACGGGTATT TGATTCTCGG GTGGATAAAA TTCGCCAGCT AAACGTCCGG 
ACCCCCACTC TCCGCACATC CATGTACCAG AAGTGCACCG CCATCGAGGA GGCCGTAAAA 
GCCATAGAGC AAAGACTGTC CAAAATAGAC CACACGGCGA TCCACCCCCA TCTGCTGGAT 
ATGAAGATAG GGCAGGGAAA GTACGAGCCT GGATTCTTCC CCAAACTGCA GTCGGATGTT 
CTTTCCACGG GACCTGCCAC AAATAAATGG ACCAAGAGGA ACGCGCCAGC ACAGTGGAGG 
AGGAAGGACA GACAGAAGCA GCACTCAGAG CACCTTAGGC TGGACAATGA CCAGAGAGAG 
AAATACATTC AAGAAGCAAG AAATATGAGC AGCGGGATCC GGCAACCCAA ACTGTCCAAC 
CTGTCACCCT CTGTAATTGC GCAGACCAAC TGGAAGTTTG TGGAGGGGCT GCTGAAGGAA 
TGTCGGAACA AGACCAAGAG AATGCTGGTG GAGAAGATGG GAAGGGAAGC GGTAGAGCTG 
GGCCACGGAG AAGTGAGCAT TACAGGGGTA GAAGAGAATG CTCTGATCGC CAGTCTGTGT 
GACCTGCTGG AGAGAATTTG GAGCCACGGT CTGCAAGTGA AGCAGGGGAA GTCAGCCTTG 
TGGTCACACT TACTGCATTA CCAGGACAGC AGGAGGAGAG CGACCTGGGG CAGTCTTGGC 
GGAACAGATT TGGTACTGGA CTGTGAGAGA AGGAAGTCTG ACCCCTTTCT GGTCATGTAC 
CCCCTAAAGA TCTCACTTGT TCAGGATATG AGGAAGCTGT ACAAGCGCTA CGCCTTCCTG 
CGCTGCGACG ATGAGAAGGA ACAGTTCCTC TATCATTTGC TCTCCTTCAA TGCTGTGGAT 
TATTTCTGCT TTACTAACGT GTTTACAACT ATCATGATCC CCTACCACAT CCTGATTGTC 
CCTAGCAAGA AGCTCGGCGG CTCCATGTTC ACAGCCAACC CCTGGATCTG TATATCTGGG 
GAGCTGGGAG AGACTGGGGT ACTGCAGGTT CCCAGGAATA TTCTAGAGAT GACGTATGAG 
TGCCAGAACC TCGGGAAGCT GACGACTGTA CAGATTGGAC ACGACAATTC TGGCCTGTAT 
GCCAAGTGGC TGGTGGAATA TGTTATGGTC CGAAATGAAA TAACTGGACA CACCTACAAG 
TTCCCTTGTG GGCGCTGGTT GGGAAAAGGA ATGGATGATG GCAGTCTGGA ACGTGTGCTG 
GTTGGAGACC TGCTTACATC CAGTACAGAC ACTGAAGAGA AACCATGCCG GACCCCTCCC 
CTGCAGCACT CACCCAGCAT GATCAGGAGG CTTGTGGCCA TATCCCCCAA TAGCCGACCA 
AAATTAAATA CGGGTCAGAT CCAGGAGGCT ATTGGAGAAG CAGTGAATGG AATTGTGAAA 
CATTTTCATA AACCAGAGAA AGAGAGAGGC AGTTTAACGT TATTACTGTG CGGAGAGAAC 
GGACTGGTGT CTGCCCTTGA ACAAGTTTTC CAGCATGGAA TCCGATCCCC TCGGCTTTTC 
AAAAGCATCT TCATCTGGGA CTTCTTAGAA AAAGCACAAG CTTATTACGA ATCTCTGGAA 
GTGAATGAAT TGGTGCCAGA AGAGGACTGG CAGAAACGGG CGCGGAATTT CTCCCGCTTT 
ATTAACGCCA TTAACAACAA CCCTAGAAAC ATTGGGAAAG ACGGCAAGTT TCAGATGTTT 
GTTTGCTTAG GAGCCCGGGA CCACCATTTG CACCACTGGA TTGCACTGCT GGCCGACTGC 
CCCATTATAG CACAAATGTA CGAGGAGACG GCTCTAATCA AGGACCACAC GTTGGTGAAC 
TCTCTAATCC GAGTGTTACA GACACTACAA GAGTTTAACA TCACCTTAGA AAGCTCCCTC 
ATTAAAGGAA TCAATATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945603.1
CDS	411..3851
Translation	MLCMPKGLAFKTQADPKEPQYHAFIITREDGSRTFGFSLTFFEEVTSKQICSAMQTLYHMHNAEYDIIHTSASNGRDCPGECTDSSITKLQRFNSYDITRDTLYVSKCICLIAPMSFMTACKKVLEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPAGRSLKFNGVYGPIVCQRPSTNELPLFDFPAQEVFELLGVENVVQLFTCALLEFQILLYSQHYQRLMTVAETITGLMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEVSEILLAFGIPPEGNLHCSESISKLRNLKAADLVSDKRNGNLAGSPLNSYELLKENETIARLQALVKRTGVSLEKQVEVREESSSKKDGKAHCDEEELRMHQLNIQVREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDNKIMCHDDDDKDPVLRVFDSRVDKIRQLNVRTPTLRTSMYQKCTAIEEAVKAIEQRLSKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQSDVLSTGPATNKWTKRNAPAQWRRKDRQKQHSEHLRLDNDQREKYIQEARNMSSGIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENALIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQDSRRRATWGSLGGTDLVLDCERRKSDPFLVMYPLKISLVQDMRKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETGVLQVPRNILEMTYECQNLGKLTTVQIGHDNSGLYAKWLVEYVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGDLLTSSTDTEEKPCRTPPLQHSPSMIRRLVAISPNSRPKLNTGQIQEAIGEAVNGIVKHFHKPEKERGSLTLLLCGENGLVSALEQVFQHGIRSPRLFKSIFIWDFLEKAQAYYESLEVNELVPEEDWQKRARNFSRFINAINNNPRNIGKDGKFQMFVCLGARDHHLHHWIALLADCPIIAQMYEETALIKDHTLVNSLIRVLQTLQEFNITLESSLIKGINI

Target ORF information:

RefSeq Version	XM_018090114.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090114.1

ORF Insert Sequence:

ATGCTATGCA TGCCTAAAGG ATTAGCATTT AAGACTCAGG CCGACCCCAA GGAGCCCCAG 
TACCACGCGT TCATCATCAC GAGAGAAGAC GGCTCTAGAA CATTTGGGTT CTCCCTCACC 
TTCTTTGAAG AAGTCACCAG CAAGCAGATA TGCAGCGCCA TGCAGACCCT GTACCACATG 
CATAACGCCG AGTATGACAT CATCCACACG TCGGCGAGCA ACGGCAGAGA CTGCCCCGGA 
GAGTGCACCG ACAGCTCCAT CACCAAGCTG CAGCGCTTCA ACTCTTATGA CATAACACGA 
GACACCCTGT ATGTGTCCAA GTGCATCTGT CTCATCGCCC CCATGTCTTT CATGACCGCC 
TGTAAGAAAG TCCTGGAGCA GCTCCACCAG GCTGTGACTT CCCCACAGCC GCCGCCATTA 
CCCTTAGAAA GTTACATTTA TAACATCCTC TATGAGGTGC CTCTGCCCCC GGCTGGGAGG 
TCCCTGAAGT TTAATGGGGT GTATGGACCA ATCGTGTGTC AGAGGCCGAG CACCAATGAG 
CTGCCCTTGT TTGACTTTCC TGCCCAGGAA GTGTTTGAGT TGCTTGGGGT GGAAAACGTG 
GTGCAGCTGT TTACGTGCGC CCTGCTGGAA TTCCAGATAT TGCTCTACTC CCAACATTAC 
CAGAGGTTAA TGACGGTGGC AGAGACCATC ACAGGCCTGA TGTTCCCATT TCAGTGGCAG 
CATGTCTACG TCCCCATCTT GCCAGCCTCC CTCCTCCATT TCCTGGACGC GCCGGTTCCG 
TACCTAATGG GATTGCACTC CAATGGGCTG GATGACAGAT CTAAGCTGGA GCTGCCACAA 
GAGGCCAACC TGTGCTTCGT GGACATAGAC AACCACTTTA TAGAGCTGCC CGAGGACCTG 
CCGCAGTTCC CCAACAAGCT GGAATTCATA CAGGAGGTAT CGGAGATCCT TTTGGCCTTC 
GGCATCCCCC CCGAGGGGAA CCTGCACTGC AGCGAGAGCA TCTCCAAGCT GAGGAACCTC 
AAGGCAGCCG ACCTGGTGTC CGACAAGAGG AACGGAAACC TGGCCGGCTC CCCATTAAAC 
TCCTATGAAC TTTTAAAAGA GAACGAGACT ATTGCCCGGC TTCAGGCGCT GGTGAAGAGG 
ACGGGGGTCA GTCTGGAAAA ACAGGTGGAA GTGCGGGAGG AGTCGAGCAG CAAAAAGGAC 
GGCAAAGCTC ACTGTGACGA GGAGGAGCTC CGAATGCACC AACTCAACAT CCAGGTCCGG 
GAAGTCTTTG CCAACCGCTT CACTCAGATG TTTGCCGATT ATGAGGTGTT TGTGATCCAG 
CCCAGCCAGG ACAAGGAATC CTGGTTCACT AACAGGGAGC AAATGCAGAA TTTTGATAAA 
GCCTCGTTCC TATCAGACCA GCCGGAGCCC TACCTCCCCT TCCTCTCCCG GTTCCTCGAG 
ACACAGATGT TTGCTTCCTT CATCGACAAT AAAATCATGT GCCACGATGA CGATGACAAG 
GATCCAGTTT TACGGGTATT TGATTCTCGG GTGGATAAAA TTCGCCAGCT AAACGTCCGG 
ACCCCCACTC TCCGCACATC CATGTACCAG AAGTGCACCG CCATCGAGGA GGCCGTAAAA 
GCCATAGAGC AAAGACTGTC CAAAATAGAC CACACGGCGA TCCACCCCCA TCTGCTGGAT 
ATGAAGATAG GGCAGGGAAA GTACGAGCCT GGATTCTTCC CCAAACTGCA GTCGGATGTT 
CTTTCCACGG GACCTGCCAC AAATAAATGG ACCAAGAGGA ACGCGCCAGC ACAGTGGAGG 
AGGAAGGACA GACAGAAGCA GCACTCAGAG CACCTTAGGC TGGACAATGA CCAGAGAGAG 
AAATACATTC AAGAAGCAAG AAATATGAGC AGCGGGATCC GGCAACCCAA ACTGTCCAAC 
CTGTCACCCT CTGTAATTGC GCAGACCAAC TGGAAGTTTG TGGAGGGGCT GCTGAAGGAA 
TGTCGGAACA AGACCAAGAG AATGCTGGTG GAGAAGATGG GAAGGGAAGC GGTAGAGCTG 
GGCCACGGAG AAGTGAGCAT TACAGGGGTA GAAGAGAATG CTCTGATCGC CAGTCTGTGT 
GACCTGCTGG AGAGAATTTG GAGCCACGGT CTGCAAGTGA AGCAGGGGAA GTCAGCCTTG 
TGGTCACACT TACTGCATTA CCAGGACAGC AGGAGGAGAG CGACCTGGGG CAGTCTTGGC 
GGAACAGATT TGGTACTGGA CTGTGAGAGA AGGAAGTCTG ACCCCTTTCT GGTCATGTAC 
CCCCTAAAGA TCTCACTTGT TCAGGATATG AGGAAGCTGT ACAAGCGCTA CGCCTTCCTG 
CGCTGCGACG ATGAGAAGGA ACAGTTCCTC TATCATTTGC TCTCCTTCAA TGCTGTGGAT 
TATTTCTGCT TTACTAACGT GTTTACAACT ATCATGATCC CCTACCACAT CCTGATTGTC 
CCTAGCAAGA AGCTCGGCGG CTCCATGTTC ACAGCCAACC CCTGGATCTG TATATCTGGG 
GAGCTGGGAG AGACTGGGGT ACTGCAGGTT CCCAGGAATA TTCTAGAGAT GACGTATGAG 
TGCCAGAACC TCGGGAAGCT GACGACTGTA CAGATTGGAC ACGACAATTC TGGCCTGTAT 
GCCAAGTGGC TGGTGGAATA TGTTATGGTC CGAAATGAAA TAACTGGACA CACCTACAAG 
TTCCCTTGTG GGCGCTGGTT GGGAAAAGGA ATGGATGATG GCAGTCTGGA ACGTGTGCTG 
GTTGGAGACC TGCTTACATC CAGTACAGAC ACTGAAGAGA AACCATGCCG GACCCCTCCC 
CTGCAGCACT CACCCAGCAT GATCAGGAGG CTTGTGGCCA TATCCCCCAA TAGCCGACCA 
AAATTAAATA CGGGTCAGAT CCAGGAGGCT ATTGGAGAAG CAGTGAATGG AATTGTGAAA 
CATTTTCATA AACCAGAGAA AGAGAGAGGC AGTTTAACGT TATTACTGTG CGGAGAGAAC 
GGACTGGTGT CTGCCCTTGA ACAAGTTTTC CAGCATGGAA TCCGATCCCC TCGGCTTTTC 
AAAAGCATCT TCATCTGGGA CTTCTTAGAA AAAGCACAAG CTTATTACGA ATCTCTGGAA 
GTGAATGAAT TGGTGCCAGA AGAGGACTGG CAGAAACGGG CGCGGAATTT CTCCCGCTTT 
ATTAACGCCA TTAACAACAA CCCTAGAAAC ATTGGGAAAG ACGGCAAGTT TCAGATGTTT 
GTTTGCTTAG GAGCCCGGGA CCACCATTTG CACCACTGGA TTGCACTGCT GGCCGACTGC 
CCCATTATAG CACAAATGTA CGAGGAGACG GCTCTAATCA AGGACCACAC GTTGGTGAAC 
TCTCTAATCC GAGTGTTACA GACACTACAA GAGTTTAACA TCACCTTAGA AAGCTCCCTC 
ATTAAAGGAA TCAATATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_018090115.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945604.1 Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa41186
Clone ID Related Accession (Same CDS sequence)	XM_018090115.1
Accession Version	XM_018090115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTATGCA TGCCTAAAGG ATTAGCATTT AAGACTCAGG CCGACCCCAA GGAGCCCCAG 
TACCACGCGT TCATCATCAC GAGAGAAGAC GGCTCTAGAA CATTTGGGTT CTCCCTCACC 
TTCTTTGAAG AAGTCACCAG CAAGCAGATA TGCAGCGCCA TGCAGACCCT GTACCACATG 
CATAACGCCG AGTATGACAT CATCCACACG TCGGCGAGCA ACGGCAGAGA CTGCCCCGGA 
GAGTGCACCG ACAGCTCCAT CACCAAGCTG CAGCGCTTCA ACTCTTATGA CATAACACGA 
GACACCCTGT ATGTGTCCAA GTGCATCTGT CTCATCGCCC CCATGTCTTT CATGACCGCC 
TGTAAGAAAG TCCTGGAGCA GCTCCACCAG GCTGTGACTT CCCCACAGCC GCCGCCATTA 
CCCTTAGAAA GTTACATTTA TAACATCCTC TATGAGGTGC CTCTGCCCCC GGCTGGGAGG 
TCCCTGAAGT TTAATGGGGT GTATGGACCA ATCGTGTGTC AGAGGCCGAG CACCAATGAG 
CTGCCCTTGT TTGACTTTCC TGCCCAGGAA GTGTTTGAGT TGCTTGGGGT GGAAAACGTG 
GTGCAGCTGT TTACGTGCGC CCTGCTGGAA TTCCAGATAT TGCTCTACTC CCAACATTAC 
CAGAGGTTAA TGACGGTGGC AGAGACCATC ACAGGCCTGA TGTTCCCATT TCAGTGGCAG 
CATGTCTACG TCCCCATCTT GCCAGCCTCC CTCCTCCATT TCCTGGACGC GCCGGTTCCG 
TACCTAATGG GATTGCACTC CAATGGGCTG GATGACAGAT CTAAGCTGGA GCTGCCACAA 
GAGGCCAACC TGTGCTTCGT GGACATAGAC AACCACTTTA TAGAGCTGCC CGAGGACCTG 
CCGCAGTTCC CCAACAAGCT GGAATTCATA CAGGAGGTAT CGGAGATCCT TTTGGCCTTC 
GGCATCCCCC CCGAGGGGAA CCTGCACTGC AGCGAGAGCA TCTCCAAGCT GAGGAACCTC 
AAGGCAGCCG ACCTGGTGTC CGACAAGAGG AACGGAAACC TGGCCGGCTC CCCATTAAAC 
TCCTATGAAC TTTTAAAAGA GAACGAGACT ATTGCCCGGC TTCAGGCGCT GGTGAAGAGG 
ACGGGGGTCA GTCTGGAAAA ACAGGTGGAA GTGCGGGAGG AGTCGAGCAG CAAAAAGGAC 
GGCAAAGCTC ACTGTGACGA GGAGGAGCTC CGAATGCACC AACTCAACAT CCAGGTCCGG 
GAAGTCTTTG CCAACCGCTT CACTCAGATG TTTGCCGATT ATGAGGTGTT TGTGATCCAG 
CCCAGCCAGG ACAAGGAATC CTGGTTCACT AACAGGGAGC AAATGCAGAA TTTTGATAAA 
GCCTCGTTCC TATCAGACCA GCCGGAGCCC TACCTCCCCT TCCTCTCCCG GTTCCTCGAG 
ACACAGATGT TTGCTTCCTT CATCGACAAT AAAATCATGT GCCACGATGA CGATGACAAG 
GATCCAGTTT TACGGGTATT TGATTCTCGG GTGGATAAAA TTCGCCAGCT AAACGTCCGG 
ACCCCCACTC TCCGCACATC CATGTACCAG AAGTGCACCG CCATCGAGGA GGCCGTAAAA 
GCCATAGAGC AAAGACTGTC CAAAATAGAC CACACGGCGA TCCACCCCCA TCTGCTGGAT 
ATGAAGATAG GGCAGGGAAA GTACGAGCCT GGATTCTTCC CCAAACTGCA GTCGGATGTT 
CTTTCCACGG GACCTGCCAC AAATAAATGG ACCAAGAGGA ACGCGCCAGC ACAGTGGAGG 
AGGAAGGACA GACAGAAGCA GCACTCAGAG CACCTTAGGC TGGACAATGA CCAGAGAGAG 
AAATACATTC AAGAAGCAAG AAATATGAGC AGCGGGATCC GGCAACCCAA ACTGTCCAAC 
CTGTCACCCT CTGTAATTGC GCAGACCAAC TGGAAGTTTG TGGAGGGGCT GCTGAAGGAA 
TGTCGGAACA AGACCAAGAG AATGCTGGTG GAGAAGATGG GAAGGGAAGC GGTAGAGCTG 
GGCCACGGAG AAGTGAGCAT TACAGGGGTA GAAGAGAATG CTCTGATCGC CAGTCTGTGT 
GACCTGCTGG AGAGAATTTG GAGCCACGGT CTGCAAGTGA AGCAGGGGAA GTCAGCCTTG 
TGGTCACACT TACTGCATTA CCAGGACAGC AGGAGGAGAG CGACCTGGGG CAGTCTTGGC 
GGAACAGATT TGGTACTGGA CTGTGAGAGA AGGAAGTCTG ACCCCTTTCT GGTCATGTAC 
CCCCTAAAGA TCTCACTTGT TCAGGATATG AGGCATATTC AGAACATTGG AGAAATCAAG 
ACAGATGTAG GGAAGGCGCG AGCCTGGGTC AGGCTGTCTA TGGAAAAGAA ACTTCTGTCC 
CGGCACCTGA AGCAGCTGCT GTTGGATCAT GATCTCAGCA AGAAGCTGTA CAAGCGCTAC 
GCCTTCCTGC GCTGCGACGA TGAGAAGGAA CAGTTCCTCT ATCATTTGCT CTCCTTCAAT 
GCTGTGGATT ATTTCTGCTT TACTAACGTG TTTACAACTA TCATGATCCC CTACCACATC 
CTGATTGTCC CTAGCAAGAA GCTCGGCGGC TCCATGTTCA CAGCCAACCC CTGGATCTGT 
ATATCTGGGG AGCTGGGAGA GACTGGGGTA CTGCAGGTTC CCAGGAATAT TCTAGAGATG 
ACGTATGAGT GCCAGAACCT CGGGAAGCTG ACGACTGTAC AGATTGGACA CGACAATTCT 
GGCCTGTATG CCAAGTGGCT GGTGGAATAT GTTATGGTCC GAAATGAAAT AACTGGACAC 
ACCTACAAGT TCCCTTGTGG GCGCTGGTTG GGAAAAGGAA TGGATGATGG CAGTCTGGAA 
CGTGTGCTGG TTGGAGACCT GCTTACATCC AGTACAGACA CTGAAGAGAA ACCATGCCGG 
ACCCCTCCCC TGCAGCACTC ACCCAGCATG ATCAGGAGGC TTGTGGCCAT ATCCCCCAAT 
AGCCGACCAA AATTAAATAC GGGTCAGATC CAGGAGGCTA TTGGAGAAGC AGTGAATGGA 
ATTGTGAAAC ATTTTCATAA ACCAGAGAAA GAGAGAGGCA GTTTAACGTT ATTACTGTGC 
GGAGAGAACG GACTGGTGTC TGCCCTTGAA CAAGTTTTCC AGCATGGAAT CCGATCCCCT 
CGGCTTTTCA AAAGCATCTT CATCTGGGAC TTCTTAGGGA CCACCATTTG CACCACTGGA 
TTGCACTGCT GGCCGACTGC CCCATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945604.1
CDS	411..3680
Translation	MLCMPKGLAFKTQADPKEPQYHAFIITREDGSRTFGFSLTFFEEVTSKQICSAMQTLYHMHNAEYDIIHTSASNGRDCPGECTDSSITKLQRFNSYDITRDTLYVSKCICLIAPMSFMTACKKVLEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPAGRSLKFNGVYGPIVCQRPSTNELPLFDFPAQEVFELLGVENVVQLFTCALLEFQILLYSQHYQRLMTVAETITGLMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEVSEILLAFGIPPEGNLHCSESISKLRNLKAADLVSDKRNGNLAGSPLNSYELLKENETIARLQALVKRTGVSLEKQVEVREESSSKKDGKAHCDEEELRMHQLNIQVREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDNKIMCHDDDDKDPVLRVFDSRVDKIRQLNVRTPTLRTSMYQKCTAIEEAVKAIEQRLSKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQSDVLSTGPATNKWTKRNAPAQWRRKDRQKQHSEHLRLDNDQREKYIQEARNMSSGIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENALIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQDSRRRATWGSLGGTDLVLDCERRKSDPFLVMYPLKISLVQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLLDHDLSKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETGVLQVPRNILEMTYECQNLGKLTTVQIGHDNSGLYAKWLVEYVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGDLLTSSTDTEEKPCRTPPLQHSPSMIRRLVAISPNSRPKLNTGQIQEAIGEAVNGIVKHFHKPEKERGSLTLLLCGENGLVSALEQVFQHGIRSPRLFKSIFIWDFLGTTICTTGLHCWPTAPL

Target ORF information:

RefSeq Version	XM_018090115.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090115.1

ORF Insert Sequence:

ATGCTATGCA TGCCTAAAGG ATTAGCATTT AAGACTCAGG CCGACCCCAA GGAGCCCCAG 
TACCACGCGT TCATCATCAC GAGAGAAGAC GGCTCTAGAA CATTTGGGTT CTCCCTCACC 
TTCTTTGAAG AAGTCACCAG CAAGCAGATA TGCAGCGCCA TGCAGACCCT GTACCACATG 
CATAACGCCG AGTATGACAT CATCCACACG TCGGCGAGCA ACGGCAGAGA CTGCCCCGGA 
GAGTGCACCG ACAGCTCCAT CACCAAGCTG CAGCGCTTCA ACTCTTATGA CATAACACGA 
GACACCCTGT ATGTGTCCAA GTGCATCTGT CTCATCGCCC CCATGTCTTT CATGACCGCC 
TGTAAGAAAG TCCTGGAGCA GCTCCACCAG GCTGTGACTT CCCCACAGCC GCCGCCATTA 
CCCTTAGAAA GTTACATTTA TAACATCCTC TATGAGGTGC CTCTGCCCCC GGCTGGGAGG 
TCCCTGAAGT TTAATGGGGT GTATGGACCA ATCGTGTGTC AGAGGCCGAG CACCAATGAG 
CTGCCCTTGT TTGACTTTCC TGCCCAGGAA GTGTTTGAGT TGCTTGGGGT GGAAAACGTG 
GTGCAGCTGT TTACGTGCGC CCTGCTGGAA TTCCAGATAT TGCTCTACTC CCAACATTAC 
CAGAGGTTAA TGACGGTGGC AGAGACCATC ACAGGCCTGA TGTTCCCATT TCAGTGGCAG 
CATGTCTACG TCCCCATCTT GCCAGCCTCC CTCCTCCATT TCCTGGACGC GCCGGTTCCG 
TACCTAATGG GATTGCACTC CAATGGGCTG GATGACAGAT CTAAGCTGGA GCTGCCACAA 
GAGGCCAACC TGTGCTTCGT GGACATAGAC AACCACTTTA TAGAGCTGCC CGAGGACCTG 
CCGCAGTTCC CCAACAAGCT GGAATTCATA CAGGAGGTAT CGGAGATCCT TTTGGCCTTC 
GGCATCCCCC CCGAGGGGAA CCTGCACTGC AGCGAGAGCA TCTCCAAGCT GAGGAACCTC 
AAGGCAGCCG ACCTGGTGTC CGACAAGAGG AACGGAAACC TGGCCGGCTC CCCATTAAAC 
TCCTATGAAC TTTTAAAAGA GAACGAGACT ATTGCCCGGC TTCAGGCGCT GGTGAAGAGG 
ACGGGGGTCA GTCTGGAAAA ACAGGTGGAA GTGCGGGAGG AGTCGAGCAG CAAAAAGGAC 
GGCAAAGCTC ACTGTGACGA GGAGGAGCTC CGAATGCACC AACTCAACAT CCAGGTCCGG 
GAAGTCTTTG CCAACCGCTT CACTCAGATG TTTGCCGATT ATGAGGTGTT TGTGATCCAG 
CCCAGCCAGG ACAAGGAATC CTGGTTCACT AACAGGGAGC AAATGCAGAA TTTTGATAAA 
GCCTCGTTCC TATCAGACCA GCCGGAGCCC TACCTCCCCT TCCTCTCCCG GTTCCTCGAG 
ACACAGATGT TTGCTTCCTT CATCGACAAT AAAATCATGT GCCACGATGA CGATGACAAG 
GATCCAGTTT TACGGGTATT TGATTCTCGG GTGGATAAAA TTCGCCAGCT AAACGTCCGG 
ACCCCCACTC TCCGCACATC CATGTACCAG AAGTGCACCG CCATCGAGGA GGCCGTAAAA 
GCCATAGAGC AAAGACTGTC CAAAATAGAC CACACGGCGA TCCACCCCCA TCTGCTGGAT 
ATGAAGATAG GGCAGGGAAA GTACGAGCCT GGATTCTTCC CCAAACTGCA GTCGGATGTT 
CTTTCCACGG GACCTGCCAC AAATAAATGG ACCAAGAGGA ACGCGCCAGC ACAGTGGAGG 
AGGAAGGACA GACAGAAGCA GCACTCAGAG CACCTTAGGC TGGACAATGA CCAGAGAGAG 
AAATACATTC AAGAAGCAAG AAATATGAGC AGCGGGATCC GGCAACCCAA ACTGTCCAAC 
CTGTCACCCT CTGTAATTGC GCAGACCAAC TGGAAGTTTG TGGAGGGGCT GCTGAAGGAA 
TGTCGGAACA AGACCAAGAG AATGCTGGTG GAGAAGATGG GAAGGGAAGC GGTAGAGCTG 
GGCCACGGAG AAGTGAGCAT TACAGGGGTA GAAGAGAATG CTCTGATCGC CAGTCTGTGT 
GACCTGCTGG AGAGAATTTG GAGCCACGGT CTGCAAGTGA AGCAGGGGAA GTCAGCCTTG 
TGGTCACACT TACTGCATTA CCAGGACAGC AGGAGGAGAG CGACCTGGGG CAGTCTTGGC 
GGAACAGATT TGGTACTGGA CTGTGAGAGA AGGAAGTCTG ACCCCTTTCT GGTCATGTAC 
CCCCTAAAGA TCTCACTTGT TCAGGATATG AGGCATATTC AGAACATTGG AGAAATCAAG 
ACAGATGTAG GGAAGGCGCG AGCCTGGGTC AGGCTGTCTA TGGAAAAGAA ACTTCTGTCC 
CGGCACCTGA AGCAGCTGCT GTTGGATCAT GATCTCAGCA AGAAGCTGTA CAAGCGCTAC 
GCCTTCCTGC GCTGCGACGA TGAGAAGGAA CAGTTCCTCT ATCATTTGCT CTCCTTCAAT 
GCTGTGGATT ATTTCTGCTT TACTAACGTG TTTACAACTA TCATGATCCC CTACCACATC 
CTGATTGTCC CTAGCAAGAA GCTCGGCGGC TCCATGTTCA CAGCCAACCC CTGGATCTGT 
ATATCTGGGG AGCTGGGAGA GACTGGGGTA CTGCAGGTTC CCAGGAATAT TCTAGAGATG 
ACGTATGAGT GCCAGAACCT CGGGAAGCTG ACGACTGTAC AGATTGGACA CGACAATTCT 
GGCCTGTATG CCAAGTGGCT GGTGGAATAT GTTATGGTCC GAAATGAAAT AACTGGACAC 
ACCTACAAGT TCCCTTGTGG GCGCTGGTTG GGAAAAGGAA TGGATGATGG CAGTCTGGAA 
CGTGTGCTGG TTGGAGACCT GCTTACATCC AGTACAGACA CTGAAGAGAA ACCATGCCGG 
ACCCCTCCCC TGCAGCACTC ACCCAGCATG ATCAGGAGGC TTGTGGCCAT ATCCCCCAAT 
AGCCGACCAA AATTAAATAC GGGTCAGATC CAGGAGGCTA TTGGAGAAGC AGTGAATGGA 
ATTGTGAAAC ATTTTCATAA ACCAGAGAAA GAGAGAGGCA GTTTAACGTT ATTACTGTGC 
GGAGAGAACG GACTGGTGTC TGCCCTTGAA CAAGTTTTCC AGCATGGAAT CCGATCCCCT 
CGGCTTTTCA AAAGCATCTT CATCTGGGAC TTCTTAGGGA CCACCATTTG CACCACTGGA 
TTGCACTGCT GGCCGACTGC CCCATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_018090117.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945606.1 Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa41187
Clone ID Related Accession (Same CDS sequence)	XM_018090117.1
Accession Version	XM_018090117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGCG GGAGAGGAGG GCGGTTTGAC AGCAGCACCA TGAGCGGGGG CGGGGGAGGC 
AGCAGCGCGG CCCCGGGCCG TTTCGCTGAT TATTTCGTGA TGTCTGGACC GGATACAAAC 
ACGGGACTGG AACCAAAGTC TCTGACAGCA TTGTGTGAGT ACATACAGGC TTCTAAAGCC 
AGAGACGGGG CTGGACAGTT TATTTCCATA ACTGCAAATG GAGCAGAAAA TTTTGAGCAG 
ACCCCTCTGC GCCGCACCTT TAAATCCAAG GTCTTGGCGC GGTACCCAGA GAACGTGGAG 
TGGAACGCCT TCGACCAGGA TGCCGTGGGC ATGCTATGCA TGCCTAAAGG ATTAGCATTT 
AAGACTCAGG CCGACCCCAA GGAGCCCCAG TACCACGCGT TCATCATCAC GAGAGAAGAC 
GGCTCTAGAA CATTTGGGTT CTCCCTCACC TTCTTTGAAG AAGTCACCAG CAAGCAGATA 
TGCAGCGCCA TGCAGACCCT GTACCACATG CATAACGCCG AGTATGACAT CATCCACACG 
TCGGCGAGCA ACGGCAGAGA CTGCCCCGGA GAGTGCACCG ACAGCTCCAT CACCAAGCTG 
CAGCGCTTCA ACTCTTATGA CATAACACGA GACACCCTGT ATGTGTCCAA GTGCATCTGT 
CTCATCGCCC CCATGTCTTT CATGACCGCC TGTAAGAAAG TCCTGGAGCA GCTCCACCAG 
GCTGTGACTT CCCCACAGCC GCCGCCATTA CCCTTAGAAA GTTACATTTA TAACATCCTC 
TATGAGGTGC CTCTGCCCCC GGCTGGGAGG TCCCTGAAGT TTAATGGGGT GTATGGACCA 
ATCGTGTGTC AGAGGCCGAG CACCAATGAG CTGCCCTTGT TTGACTTTCC TGCCCAGGAA 
GTGTTTGAGT TGCTTGGGGT GGAAAACGTG GTGCAGCTGT TTACGTGCGC CCTGCTGGAA 
TTCCAGATAT TGCTCTACTC CCAACATTAC CAGAGGTTAA TGACGGTGGC AGAGACCATC 
ACAGGCCTGA TGTTCCCATT TCAGTGGCAG CATGTCTACG TCCCCATCTT GCCAGCCTCC 
CTCCTCCATT TCCTGGACGC GCCGGTTCCG TACCTAATGG GATTGCACTC CAATGGGCTG 
GATGACAGAT CTAAGCTGGA GCTGCCACAA GAGGCCAACC TGTGCTTCGT GGACATAGAC 
AACCACTTTA TAGAGCTGCC CGAGGACCTG CCGCAGTTCC CCAACAAGCT GGAATTCATA 
CAGGAGGTAT CGGAGATCCT TTTGGCCTTC GGCATCCCCC CCGAGGGGAA CCTGCACTGC 
AGCGAGAGCA TCTCCAAGCT GAGGAACCTC AAGGCAGCCG ACCTGGTGTC CGACAAGAGG 
AACGGAAACC TGGCCGGCTC CCCATTAAAC TCCTATGAAC TTTTAAAAGA GAACGAGACT 
ATTGCCCGGC TTCAGGCGCT GGTGAAGAGG ACGGGGGTCA GTCTGGAAAA ACAGGTGGAA 
GTGCGGGAGG AGTCGAGCAG CAAAAAGGAC GGCAAAGCTC ACTGTGACGA GGAGGAGCTC 
CGAATGCACC AACTCAACAT CCAGGTCCGG GAAGTCTTTG CCAACCGCTT CACTCAGATG 
TTTGCCGATT ATGAGGTGTT TGTGATCCAG CCCAGCCAGG ACAAGGAATC CTGGTTCACT 
AACAGGGAGC AAATGCAGAA TTTTGATAAA GCCTCGTTCC TATCAGACCA GCCGGAGCCC 
TACCTCCCCT TCCTCTCCCG GTTCCTCGAG ACACAGATGT TTGCTTCCTT CATCGACAAT 
AAAATCATGT GCCACGATGA CGATGACAAG GATCCAGTTT TACGGGTATT TGATTCTCGG 
GTGGATAAAA TTCGCCAGCT AAACGTCCGG ACCCCCACTC TCCGCACATC CATGTACCAG 
AAGTGCACCG CCATCGAGGA GGCCGTAAAA GCCATAGAGC AAAGACTGTC CAAAATAGAC 
CACACGGCGA TCCACCCCCA TCTGCTGGAT ATGAAGATAG GGCAGGGAAA GTACGAGCCT 
GGATTCTTCC CCAAACTGCA GTCGGATGTT CTTTCCACGG GACCTGCCAC AAATAAATGG 
ACCAAGAGGA ACGCGCCAGC ACAGTGGAGG AGGAAGGACA GACAGAAGCA GCACTCAGAG 
CACCTTAGGC TGGACAATGA CCAGAGAGAG AAATACATTC AAGAAGCAAG AAATATGAGC 
AGCGGGATCC GGCAACCCAA ACTGTCCAAC CTGTCACCCT CTGTAATTGC GCAGACCAAC 
TGGAAGTTTG TGGAGGGGCT GCTGAAGGAA TGTCGGAACA AGACCAAGAG AATGCTGGTG 
GAGAAGATGG GAAGGGAAGC GGTAGAGCTG GGCCACGGAG AAGTGAGCAT TACAGGGGTA 
GAAGAGAATG CTCTGATCGC CAGTCTGTGT GACCTGCTGG AGAGAATTTG GAGCCACGGT 
CTGCAAGTGA AGCAGGGGAA GTCAGCCTTG TGGTCACACT TACTGCATTA CCAGGACAGC 
AGGAGGAGAG CGACCTGGGG CAGTCTTGGC GGAACAGATT TGGTACTGGA CTGTGAGAGA 
AGGAAGTCTG ACCCCTTTCT GGTCATGTAC CCCCTAAAGA TCTCACTTGT TCAGGATATG 
AGGCATATTC AGAACATTGG AGAAATCAAG ACAGATGTAG GGAAGGCGCG AGCCTGGGTC 
AGGCTGTCTA TGGAAAAGAA ACTTCTGTCC CGGCACCTGA AGCAGCTGCT GTTGGATCAT 
GATCTCAGCA AGAAGCTGTA CAAGCGCTAC GCCTTCCTGC GCTGCGACGA TGAGAAGGAA 
CAGTTCCTCT ATCATTTGCT CTCCTTCAAT GCTGTGGATT ATTTCTGCTT TACTAACGTG 
TTTACAACTA TCATGATCCC CTACCACATC CTGATTGTCC CTAGCAAGAA GCTCGGCGGC 
TCCATGTTCA CAGCCAACCC CTGGATCTGT ATATCTGGGG AGCTGGGAGA GACTGGGGTA 
CTGCAGGTTC CCAGGAATAT TCTAGAGATG ACGTATGAGT GCCAGAACCT CGGGAAGCTG 
ACGACTGTAC AGATTGGACA CGACAATTCT GGCCTGTATG CCAAGTGGCT GGTGGAATAT 
GTTATGGTCC GAAATGAAAT AACTGGACAC ACCTACAAGT TCCCTTGTGG GCGCTGGTTG 
GGAAAAGGAA TGGATGATGG CAGTCTGGAA CGTGTGCTGG TTGGAGACCT GCTTACATCC 
AGTACAGACA CTGAAGAGAA ACCATGCCGG ACCCCTCCCC TGCAGCACTC ACCCAGCATG 
ATCAGGAGGC TTGTGGCCAT ATCCCCCAAT AGCCGACCAA AATTAAATAC GGGTCAGATC 
CAGGAGGCTA TTGGAGAAGC AGTGAATGGA ATTGTGAAAC ATTTTCATAA ACCAGAGAAA 
GAGAGAGGCA GTTTAACGTT ATTACTGTGC GGAGAGAACG GACTGGTGTC TGCCCTTGAA 
CAAGTTTTCC AGCATGGAAT CCGATCCCCT CGGCTTTTCA AAAGCATCTT CATCTGGGAC 
TTCTTAGAAA AAGCACAAGC TTATTACGAA TCTCTGGAAG TGAATGAATT GGTGCCAGAA 
GAGGACTGGC AGAAACGGGC GCGGAATTTC TCCCGCTTTA TTAACGCCAT TAACAACAAC 
CCTAGAAACA TTGGGAAAGA CGGCAAGTTT CAGATGTTTG TTTGCTTAGG AGCCCGGGAC 
CACCATTTGC ACCACTGGAT TGCACTGCTG GCCGACTGCC CCATTATAGC ACAAATGTAC 
GAGGAGACGG CTCTAATCAA GGACCACACG TTGGTGAACT CTCTAATCCG AGTGTTACAG 
ACACTACAAG AGTTTAACAT CACCTTAGAA AGCTCCCTCA TTAAAGGAAT CAATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945606.1
CDS	123..4022
Translation	MTGGRGGRFDSSTMSGGGGGSSAAPGRFADYFVMSGPDTNTGLEPKSLTALCEYIQASKARDGAGQFISITANGAENFEQTPLRRTFKSKVLARYPENVEWNAFDQDAVGMLCMPKGLAFKTQADPKEPQYHAFIITREDGSRTFGFSLTFFEEVTSKQICSAMQTLYHMHNAEYDIIHTSASNGRDCPGECTDSSITKLQRFNSYDITRDTLYVSKCICLIAPMSFMTACKKVLEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPAGRSLKFNGVYGPIVCQRPSTNELPLFDFPAQEVFELLGVENVVQLFTCALLEFQILLYSQHYQRLMTVAETITGLMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEVSEILLAFGIPPEGNLHCSESISKLRNLKAADLVSDKRNGNLAGSPLNSYELLKENETIARLQALVKRTGVSLEKQVEVREESSSKKDGKAHCDEEELRMHQLNIQVREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDNKIMCHDDDDKDPVLRVFDSRVDKIRQLNVRTPTLRTSMYQKCTAIEEAVKAIEQRLSKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQSDVLSTGPATNKWTKRNAPAQWRRKDRQKQHSEHLRLDNDQREKYIQEARNMSSGIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENALIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQDSRRRATWGSLGGTDLVLDCERRKSDPFLVMYPLKISLVQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLLDHDLSKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETGVLQVPRNILEMTYECQNLGKLTTVQIGHDNSGLYAKWLVEYVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGDLLTSSTDTEEKPCRTPPLQHSPSMIRRLVAISPNSRPKLNTGQIQEAIGEAVNGIVKHFHKPEKERGSLTLLLCGENGLVSALEQVFQHGIRSPRLFKSIFIWDFLEKAQAYYESLEVNELVPEEDWQKRARNFSRFINAINNNPRNIGKDGKFQMFVCLGARDHHLHHWIALLADCPIIAQMYEETALIKDHTLVNSLIRVLQTLQEFNITLESSLIKGINI

Target ORF information:

RefSeq Version	XM_018090117.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090117.1

ORF Insert Sequence:

ATGACAGGCG GGAGAGGAGG GCGGTTTGAC AGCAGCACCA TGAGCGGGGG CGGGGGAGGC 
AGCAGCGCGG CCCCGGGCCG TTTCGCTGAT TATTTCGTGA TGTCTGGACC GGATACAAAC 
ACGGGACTGG AACCAAAGTC TCTGACAGCA TTGTGTGAGT ACATACAGGC TTCTAAAGCC 
AGAGACGGGG CTGGACAGTT TATTTCCATA ACTGCAAATG GAGCAGAAAA TTTTGAGCAG 
ACCCCTCTGC GCCGCACCTT TAAATCCAAG GTCTTGGCGC GGTACCCAGA GAACGTGGAG 
TGGAACGCCT TCGACCAGGA TGCCGTGGGC ATGCTATGCA TGCCTAAAGG ATTAGCATTT 
AAGACTCAGG CCGACCCCAA GGAGCCCCAG TACCACGCGT TCATCATCAC GAGAGAAGAC 
GGCTCTAGAA CATTTGGGTT CTCCCTCACC TTCTTTGAAG AAGTCACCAG CAAGCAGATA 
TGCAGCGCCA TGCAGACCCT GTACCACATG CATAACGCCG AGTATGACAT CATCCACACG 
TCGGCGAGCA ACGGCAGAGA CTGCCCCGGA GAGTGCACCG ACAGCTCCAT CACCAAGCTG 
CAGCGCTTCA ACTCTTATGA CATAACACGA GACACCCTGT ATGTGTCCAA GTGCATCTGT 
CTCATCGCCC CCATGTCTTT CATGACCGCC TGTAAGAAAG TCCTGGAGCA GCTCCACCAG 
GCTGTGACTT CCCCACAGCC GCCGCCATTA CCCTTAGAAA GTTACATTTA TAACATCCTC 
TATGAGGTGC CTCTGCCCCC GGCTGGGAGG TCCCTGAAGT TTAATGGGGT GTATGGACCA 
ATCGTGTGTC AGAGGCCGAG CACCAATGAG CTGCCCTTGT TTGACTTTCC TGCCCAGGAA 
GTGTTTGAGT TGCTTGGGGT GGAAAACGTG GTGCAGCTGT TTACGTGCGC CCTGCTGGAA 
TTCCAGATAT TGCTCTACTC CCAACATTAC CAGAGGTTAA TGACGGTGGC AGAGACCATC 
ACAGGCCTGA TGTTCCCATT TCAGTGGCAG CATGTCTACG TCCCCATCTT GCCAGCCTCC 
CTCCTCCATT TCCTGGACGC GCCGGTTCCG TACCTAATGG GATTGCACTC CAATGGGCTG 
GATGACAGAT CTAAGCTGGA GCTGCCACAA GAGGCCAACC TGTGCTTCGT GGACATAGAC 
AACCACTTTA TAGAGCTGCC CGAGGACCTG CCGCAGTTCC CCAACAAGCT GGAATTCATA 
CAGGAGGTAT CGGAGATCCT TTTGGCCTTC GGCATCCCCC CCGAGGGGAA CCTGCACTGC 
AGCGAGAGCA TCTCCAAGCT GAGGAACCTC AAGGCAGCCG ACCTGGTGTC CGACAAGAGG 
AACGGAAACC TGGCCGGCTC CCCATTAAAC TCCTATGAAC TTTTAAAAGA GAACGAGACT 
ATTGCCCGGC TTCAGGCGCT GGTGAAGAGG ACGGGGGTCA GTCTGGAAAA ACAGGTGGAA 
GTGCGGGAGG AGTCGAGCAG CAAAAAGGAC GGCAAAGCTC ACTGTGACGA GGAGGAGCTC 
CGAATGCACC AACTCAACAT CCAGGTCCGG GAAGTCTTTG CCAACCGCTT CACTCAGATG 
TTTGCCGATT ATGAGGTGTT TGTGATCCAG CCCAGCCAGG ACAAGGAATC CTGGTTCACT 
AACAGGGAGC AAATGCAGAA TTTTGATAAA GCCTCGTTCC TATCAGACCA GCCGGAGCCC 
TACCTCCCCT TCCTCTCCCG GTTCCTCGAG ACACAGATGT TTGCTTCCTT CATCGACAAT 
AAAATCATGT GCCACGATGA CGATGACAAG GATCCAGTTT TACGGGTATT TGATTCTCGG 
GTGGATAAAA TTCGCCAGCT AAACGTCCGG ACCCCCACTC TCCGCACATC CATGTACCAG 
AAGTGCACCG CCATCGAGGA GGCCGTAAAA GCCATAGAGC AAAGACTGTC CAAAATAGAC 
CACACGGCGA TCCACCCCCA TCTGCTGGAT ATGAAGATAG GGCAGGGAAA GTACGAGCCT 
GGATTCTTCC CCAAACTGCA GTCGGATGTT CTTTCCACGG GACCTGCCAC AAATAAATGG 
ACCAAGAGGA ACGCGCCAGC ACAGTGGAGG AGGAAGGACA GACAGAAGCA GCACTCAGAG 
CACCTTAGGC TGGACAATGA CCAGAGAGAG AAATACATTC AAGAAGCAAG AAATATGAGC 
AGCGGGATCC GGCAACCCAA ACTGTCCAAC CTGTCACCCT CTGTAATTGC GCAGACCAAC 
TGGAAGTTTG TGGAGGGGCT GCTGAAGGAA TGTCGGAACA AGACCAAGAG AATGCTGGTG 
GAGAAGATGG GAAGGGAAGC GGTAGAGCTG GGCCACGGAG AAGTGAGCAT TACAGGGGTA 
GAAGAGAATG CTCTGATCGC CAGTCTGTGT GACCTGCTGG AGAGAATTTG GAGCCACGGT 
CTGCAAGTGA AGCAGGGGAA GTCAGCCTTG TGGTCACACT TACTGCATTA CCAGGACAGC 
AGGAGGAGAG CGACCTGGGG CAGTCTTGGC GGAACAGATT TGGTACTGGA CTGTGAGAGA 
AGGAAGTCTG ACCCCTTTCT GGTCATGTAC CCCCTAAAGA TCTCACTTGT TCAGGATATG 
AGGCATATTC AGAACATTGG AGAAATCAAG ACAGATGTAG GGAAGGCGCG AGCCTGGGTC 
AGGCTGTCTA TGGAAAAGAA ACTTCTGTCC CGGCACCTGA AGCAGCTGCT GTTGGATCAT 
GATCTCAGCA AGAAGCTGTA CAAGCGCTAC GCCTTCCTGC GCTGCGACGA TGAGAAGGAA 
CAGTTCCTCT ATCATTTGCT CTCCTTCAAT GCTGTGGATT ATTTCTGCTT TACTAACGTG 
TTTACAACTA TCATGATCCC CTACCACATC CTGATTGTCC CTAGCAAGAA GCTCGGCGGC 
TCCATGTTCA CAGCCAACCC CTGGATCTGT ATATCTGGGG AGCTGGGAGA GACTGGGGTA 
CTGCAGGTTC CCAGGAATAT TCTAGAGATG ACGTATGAGT GCCAGAACCT CGGGAAGCTG 
ACGACTGTAC AGATTGGACA CGACAATTCT GGCCTGTATG CCAAGTGGCT GGTGGAATAT 
GTTATGGTCC GAAATGAAAT AACTGGACAC ACCTACAAGT TCCCTTGTGG GCGCTGGTTG 
GGAAAAGGAA TGGATGATGG CAGTCTGGAA CGTGTGCTGG TTGGAGACCT GCTTACATCC 
AGTACAGACA CTGAAGAGAA ACCATGCCGG ACCCCTCCCC TGCAGCACTC ACCCAGCATG 
ATCAGGAGGC TTGTGGCCAT ATCCCCCAAT AGCCGACCAA AATTAAATAC GGGTCAGATC 
CAGGAGGCTA TTGGAGAAGC AGTGAATGGA ATTGTGAAAC ATTTTCATAA ACCAGAGAAA 
GAGAGAGGCA GTTTAACGTT ATTACTGTGC GGAGAGAACG GACTGGTGTC TGCCCTTGAA 
CAAGTTTTCC AGCATGGAAT CCGATCCCCT CGGCTTTTCA AAAGCATCTT CATCTGGGAC 
TTCTTAGAAA AAGCACAAGC TTATTACGAA TCTCTGGAAG TGAATGAATT GGTGCCAGAA 
GAGGACTGGC AGAAACGGGC GCGGAATTTC TCCCGCTTTA TTAACGCCAT TAACAACAAC 
CCTAGAAACA TTGGGAAAGA CGGCAAGTTT CAGATGTTTG TTTGCTTAGG AGCCCGGGAC 
CACCATTTGC ACCACTGGAT TGCACTGCTG GCCGACTGCC CCATTATAGC ACAAATGTAC 
GAGGAGACGG CTCTAATCAA GGACCACACG TTGGTGAACT CTCTAATCCG AGTGTTACAG 
ACACTACAAG AGTTTAACAT CACCTTAGAA AGCTCCCTCA TTAAAGGAAT CAATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_031899879.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755739.1 Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.

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CloneID	OXa55809
Clone ID Related Accession (Same CDS sequence)	XM_031899879.1
Accession Version	XM_031899879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGCG GGAGAGGAGG GCGGTTTGAC AGCAGCACCA TGAGCGGGGG CGGGGGAGGC 
AGCAGCGCGG CCCCGGGCCG TTTCGCTGAT TATTTCGTGA TCTGTGGGCT GGATACGGGC 
ACCGGGCTGG AGCCGGACGA GTTGTCTGGA GAGAATTTTG AGCAGACCCC TCTGCGCCGC 
ACCTTTAAAT CCAAGGTCTT GGCGCGGTAC CCAGAGAACG TGGAGTGGAA CGCCTTCGAC 
CAGGATGCCG TGGGCATGCT ATGCATGCCT AAAGGATTAG CATTTAAGAC TCAGGCCGAC 
CCCAAGGAGC CCCAGTACCA CGCGTTCATC ATCACGAGAG AAGACGGCTC TAGAACATTT 
GGGTTCTCCC TCACCTTCTT TGAAGAAGTC ACCAGCAAGC AGATATGCAG CGCCATGCAG 
ACCCTGTACC ACATGCATAA CGCCGAGTAT GACATCATCC ACACGTCGGC GAGCAACGGC 
AGAGACTGCC CCGGAGAGTG CACCGACAGC TCCATCACCA AGCTGCAGCG CTTCAACTCT 
TATGACATAA CACGAGACAC CCTGTATGTG TCCAAGTGCA TCTGTCTCAT CGCCCCCATG 
TCTTTCATGA CCGCCTGTAA GAAAGTCCTG GAGCAGCTCC ACCAGGCTGT GACTTCCCCA 
CAGCCGCCGC CATTACCCTT AGAAAGTTAC ATTTATAACA TCCTCTATGA GGTGCCTCTG 
CCCCCGGCTG GGAGGTCCCT GAAGTTTAAT GGGGTGTATG GACCAATCGT GTGTCAGAGG 
CCGAGCACCA ATGAGCTGCC CTTGTTTGAC TTTCCTGCCC AGGAAGTGTT TGAGTTGCTT 
GGGGTGGAAA ACGTGGTGCA GCTGTTTACG TGCGCCCTGC TGGAATTCCA GATATTGCTC 
TACTCCCAAC ATTACCAGAG GTTAATGACG GTGGCAGAGA CCATCACAGG CCTGATGTTC 
CCATTTCAGT GGCAGCATGT CTACGTCCCC ATCTTGCCAG CCTCCCTCCT CCATTTCCTG 
GACGCGCCGG TTCCGTACCT AATGGGATTG CACTCCAATG GGCTGGATGA CAGATCTAAG 
CTGGAGCTGC CACAAGAGGC CAACCTGTGC TTCGTGGACA TAGACAACCA CTTTATAGAG 
CTGCCCGAGG ACCTGCCGCA GTTCCCCAAC AAGCTGGAAT TCATACAGGA GGTATCGGAG 
ATCCTTTTGG CCTTCGGCAT CCCCCCCGAG GGGAACCTGC ACTGCAGCGA GAGCATCTCC 
AAGCTGAGGA ACCTCAAGGC AGCCGACCTG GTGTCCGACA AGAGGAACGG AAACCTGGCC 
GGCTCCCCAT TAAACTCCTA TGAACTTTTA AAAGAGAACG AGACTATTGC CCGGCTTCAG 
GCGCTGGTGA AGAGGACGGG GGTCAGTCTG GAAAAACAGG TGGAAGTGCG GGAGGAGTCG 
AGCAGCAAAA AGGACGGCAA AGCTCACTGT GACGAGGAGG AGCTCCGAAT GCACCAACTC 
AACATCCAGG TCCGGGAAGT CTTTGCCAAC CGCTTCACTC AGATGTTTGC CGATTATGAG 
GTGTTTGTGA TCCAGCCCAG CCAGGACAAG GAATCCTGGT TCACTAACAG GGAGCAAATG 
CAGAATTTTG ATAAAGCCTC GTTCCTATCA GACCAGCCGG AGCCCTACCT CCCCTTCCTC 
TCCCGGTTCC TCGAGACACA GATGTTTGCT TCCTTCATCG ACAATAAAAT CATGTGCCAC 
GATGACGATG ACAAGGATCC AGTTTTACGG GTATTTGATT CTCGGGTGGA TAAAATTCGC 
CAGCTAAACG TCCGGACCCC CACTCTCCGC ACATCCATGT ACCAGAAGTG CACCGCCATC 
GAGGAGGCCG TAAAAGCCAT AGAGCAAAGA CTGTCCAAAA TAGACCACAC GGCGATCCAC 
CCCCATCTGC TGGATATGAA GATAGGGCAG GGAAAGTACG AGCCTGGATT CTTCCCCAAA 
CTGCAGTCGG ATGTTCTTTC CACGGGACCT GCCACAAATA AATGGACCAA GAGGAACGCG 
CCAGCACAGT GGAGGAGGAA GGACAGACAG AAGCAGCACT CAGAGCACCT TAGGCTGGAC 
AATGACCAGA GAGAGAAATA CATTCAAGAA GCAAGAAATA TGAGCAGCGG GATCCGGCAA 
CCCAAACTGT CCAACCTGTC ACCCTCTGTA ATTGCGCAGA CCAACTGGAA GTTTGTGGAG 
GGGCTGCTGA AGGAATGTCG GAACAAGACC AAGAGAATGC TGGTGGAGAA GATGGGAAGG 
GAAGCGGTAG AGCTGGGCCA CGGAGAAGTG AGCATTACAG GGGTAGAAGA GAATGCTCTG 
ATCGCCAGTC TGTGTGACCT GCTGGAGAGA ATTTGGAGCC ACGGTCTGCA AGTGAAGCAG 
GGGAAGTCAG CCTTGTGGTC ACACTTACTG CATTACCAGG ACAGCAGGAG GAGAGCGACC 
TGGGGCAGTC TTGGCGGAAC AGATTTGGTA CTGGACTGTG AGAGAAGGAA GTCTGACCCC 
TTTCTGGTCA TGTACCCCCT AAAGATCTCA CTTGTTCAGG ATATGAGGCA TATTCAGAAC 
ATTGGAGAAA TCAAGACAGA TGTAGGGAAG GCGCGAGCCT GGGTCAGGCT GTCTATGGAA 
AAGAAACTTC TGTCCCGGCA CCTGAAGCAG CTGCTGTTGG ATCATGATCT CAGCAAGAAG 
CTGTACAAGC GCTACGCCTT CCTGCGCTGC GACGATGAGA AGGAACAGTT CCTCTATCAT 
TTGCTCTCCT TCAATGCTGT GGATTATTTC TGCTTTACTA ACGTGTTTAC AACTATCATG 
ATCCCCTACC ACATCCTGAT TGTCCCTAGC AAGAAGCTCG GCGGCTCCAT GTTCACAGCC 
AACCCCTGGA TCTGTATATC TGGGGAGCTG GGAGAGACTG GGGTACTGCA GGTTCCCAGG 
AATATTCTAG AGATGACGTA TGAGTGCCAG AACCTCGGGA AGCTGACGAC TGTACAGATT 
GGACACGACA ATTCTGGCCT GTATGCCAAG TGGCTGGTGG AATATGTTAT GGTCCGAAAT 
GAAATAACTG GACACACCTA CAAGTTCCCT TGTGGGCGCT GGTTGGGAAA AGGAATGGAT 
GATGGCAGTC TGGAACGTGT GCTGGTTGGA GACCTGCTTA CATCCAGTAC AGACACTGAA 
GAGAAACCAT GCCGGACCCC TCCCCTGCAG CACTCACCCA GCATGATCAG GAGGCTTGTG 
GCCATATCCC CCAATAGCCG ACCAAAATTA AATACGGGTC AGATCCAGGA GGCTATTGGA 
GAAGCAGTGA ATGGAATTGT GAAACATTTT CATAAACCAG AGAAAGAGAG AGGCAGTTTA 
ACGTTATTAC TGTGCGGAGA GAACGGACTG GTGTCTGCCC TTGAACAAGT TTTCCAGCAT 
GGAATCCGAT CCCCTCGGCT TTTCAAAAGC ATCTTCATCT GGGACTTCTT AGAAAAAGCA 
CAAGCTTATT ACGAATCTCT GGAAGTGAAT GAATTGGTGC CAGAAGAGGA CTGGCAGAAA 
CGGGCGCGGA ATTTCTCCCG CTTTATTAAC GCCATTAACA ACAACCCTAG AAACATTGGG 
AAAGACGGCA AGTTTCAGAT GTTTGTTTGC TTAGGAGCCC GGGACCACCA TTTGCACCAC 
TGGATTGCAC TGCTGGCCGA CTGCCCCATT ATAGCACAAA TGTACGAGGA GACGGCTCTA 
ATCAAGGACC ACACGTTGGT GAACTCTCTA ATCCGAGTGT TACAGACACT ACAAGAGTTT 
AACATCACCT TAGAAAGCTC CCTCATTAAA GGAATCAATA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755739.1
CDS	116..3940
Translation	MTGGRGGRFDSSTMSGGGGGSSAAPGRFADYFVICGLDTGTGLEPDELSGENFEQTPLRRTFKSKVLARYPENVEWNAFDQDAVGMLCMPKGLAFKTQADPKEPQYHAFIITREDGSRTFGFSLTFFEEVTSKQICSAMQTLYHMHNAEYDIIHTSASNGRDCPGECTDSSITKLQRFNSYDITRDTLYVSKCICLIAPMSFMTACKKVLEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPAGRSLKFNGVYGPIVCQRPSTNELPLFDFPAQEVFELLGVENVVQLFTCALLEFQILLYSQHYQRLMTVAETITGLMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEVSEILLAFGIPPEGNLHCSESISKLRNLKAADLVSDKRNGNLAGSPLNSYELLKENETIARLQALVKRTGVSLEKQVEVREESSSKKDGKAHCDEEELRMHQLNIQVREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDNKIMCHDDDDKDPVLRVFDSRVDKIRQLNVRTPTLRTSMYQKCTAIEEAVKAIEQRLSKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQSDVLSTGPATNKWTKRNAPAQWRRKDRQKQHSEHLRLDNDQREKYIQEARNMSSGIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENALIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQDSRRRATWGSLGGTDLVLDCERRKSDPFLVMYPLKISLVQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLLDHDLSKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETGVLQVPRNILEMTYECQNLGKLTTVQIGHDNSGLYAKWLVEYVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGDLLTSSTDTEEKPCRTPPLQHSPSMIRRLVAISPNSRPKLNTGQIQEAIGEAVNGIVKHFHKPEKERGSLTLLLCGENGLVSALEQVFQHGIRSPRLFKSIFIWDFLEKAQAYYESLEVNELVPEEDWQKRARNFSRFINAINNNPRNIGKDGKFQMFVCLGARDHHLHHWIALLADCPIIAQMYEETALIKDHTLVNSLIRVLQTLQEFNITLESSLIKGINI

Target ORF information:

RefSeq Version	XM_031899879.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899879.1

ORF Insert Sequence:

ATGACAGGCG GGAGAGGAGG GCGGTTTGAC AGCAGCACCA TGAGCGGGGG CGGGGGAGGC 
AGCAGCGCGG CCCCGGGCCG TTTCGCTGAT TATTTCGTGA TCTGTGGGCT GGATACGGGC 
ACCGGGCTGG AGCCGGACGA GTTGTCTGGA GAGAATTTTG AGCAGACCCC TCTGCGCCGC 
ACCTTTAAAT CCAAGGTCTT GGCGCGGTAC CCAGAGAACG TGGAGTGGAA CGCCTTCGAC 
CAGGATGCCG TGGGCATGCT ATGCATGCCT AAAGGATTAG CATTTAAGAC TCAGGCCGAC 
CCCAAGGAGC CCCAGTACCA CGCGTTCATC ATCACGAGAG AAGACGGCTC TAGAACATTT 
GGGTTCTCCC TCACCTTCTT TGAAGAAGTC ACCAGCAAGC AGATATGCAG CGCCATGCAG 
ACCCTGTACC ACATGCATAA CGCCGAGTAT GACATCATCC ACACGTCGGC GAGCAACGGC 
AGAGACTGCC CCGGAGAGTG CACCGACAGC TCCATCACCA AGCTGCAGCG CTTCAACTCT 
TATGACATAA CACGAGACAC CCTGTATGTG TCCAAGTGCA TCTGTCTCAT CGCCCCCATG 
TCTTTCATGA CCGCCTGTAA GAAAGTCCTG GAGCAGCTCC ACCAGGCTGT GACTTCCCCA 
CAGCCGCCGC CATTACCCTT AGAAAGTTAC ATTTATAACA TCCTCTATGA GGTGCCTCTG 
CCCCCGGCTG GGAGGTCCCT GAAGTTTAAT GGGGTGTATG GACCAATCGT GTGTCAGAGG 
CCGAGCACCA ATGAGCTGCC CTTGTTTGAC TTTCCTGCCC AGGAAGTGTT TGAGTTGCTT 
GGGGTGGAAA ACGTGGTGCA GCTGTTTACG TGCGCCCTGC TGGAATTCCA GATATTGCTC 
TACTCCCAAC ATTACCAGAG GTTAATGACG GTGGCAGAGA CCATCACAGG CCTGATGTTC 
CCATTTCAGT GGCAGCATGT CTACGTCCCC ATCTTGCCAG CCTCCCTCCT CCATTTCCTG 
GACGCGCCGG TTCCGTACCT AATGGGATTG CACTCCAATG GGCTGGATGA CAGATCTAAG 
CTGGAGCTGC CACAAGAGGC CAACCTGTGC TTCGTGGACA TAGACAACCA CTTTATAGAG 
CTGCCCGAGG ACCTGCCGCA GTTCCCCAAC AAGCTGGAAT TCATACAGGA GGTATCGGAG 
ATCCTTTTGG CCTTCGGCAT CCCCCCCGAG GGGAACCTGC ACTGCAGCGA GAGCATCTCC 
AAGCTGAGGA ACCTCAAGGC AGCCGACCTG GTGTCCGACA AGAGGAACGG AAACCTGGCC 
GGCTCCCCAT TAAACTCCTA TGAACTTTTA AAAGAGAACG AGACTATTGC CCGGCTTCAG 
GCGCTGGTGA AGAGGACGGG GGTCAGTCTG GAAAAACAGG TGGAAGTGCG GGAGGAGTCG 
AGCAGCAAAA AGGACGGCAA AGCTCACTGT GACGAGGAGG AGCTCCGAAT GCACCAACTC 
AACATCCAGG TCCGGGAAGT CTTTGCCAAC CGCTTCACTC AGATGTTTGC CGATTATGAG 
GTGTTTGTGA TCCAGCCCAG CCAGGACAAG GAATCCTGGT TCACTAACAG GGAGCAAATG 
CAGAATTTTG ATAAAGCCTC GTTCCTATCA GACCAGCCGG AGCCCTACCT CCCCTTCCTC 
TCCCGGTTCC TCGAGACACA GATGTTTGCT TCCTTCATCG ACAATAAAAT CATGTGCCAC 
GATGACGATG ACAAGGATCC AGTTTTACGG GTATTTGATT CTCGGGTGGA TAAAATTCGC 
CAGCTAAACG TCCGGACCCC CACTCTCCGC ACATCCATGT ACCAGAAGTG CACCGCCATC 
GAGGAGGCCG TAAAAGCCAT AGAGCAAAGA CTGTCCAAAA TAGACCACAC GGCGATCCAC 
CCCCATCTGC TGGATATGAA GATAGGGCAG GGAAAGTACG AGCCTGGATT CTTCCCCAAA 
CTGCAGTCGG ATGTTCTTTC CACGGGACCT GCCACAAATA AATGGACCAA GAGGAACGCG 
CCAGCACAGT GGAGGAGGAA GGACAGACAG AAGCAGCACT CAGAGCACCT TAGGCTGGAC 
AATGACCAGA GAGAGAAATA CATTCAAGAA GCAAGAAATA TGAGCAGCGG GATCCGGCAA 
CCCAAACTGT CCAACCTGTC ACCCTCTGTA ATTGCGCAGA CCAACTGGAA GTTTGTGGAG 
GGGCTGCTGA AGGAATGTCG GAACAAGACC AAGAGAATGC TGGTGGAGAA GATGGGAAGG 
GAAGCGGTAG AGCTGGGCCA CGGAGAAGTG AGCATTACAG GGGTAGAAGA GAATGCTCTG 
ATCGCCAGTC TGTGTGACCT GCTGGAGAGA ATTTGGAGCC ACGGTCTGCA AGTGAAGCAG 
GGGAAGTCAG CCTTGTGGTC ACACTTACTG CATTACCAGG ACAGCAGGAG GAGAGCGACC 
TGGGGCAGTC TTGGCGGAAC AGATTTGGTA CTGGACTGTG AGAGAAGGAA GTCTGACCCC 
TTTCTGGTCA TGTACCCCCT AAAGATCTCA CTTGTTCAGG ATATGAGGCA TATTCAGAAC 
ATTGGAGAAA TCAAGACAGA TGTAGGGAAG GCGCGAGCCT GGGTCAGGCT GTCTATGGAA 
AAGAAACTTC TGTCCCGGCA CCTGAAGCAG CTGCTGTTGG ATCATGATCT CAGCAAGAAG 
CTGTACAAGC GCTACGCCTT CCTGCGCTGC GACGATGAGA AGGAACAGTT CCTCTATCAT 
TTGCTCTCCT TCAATGCTGT GGATTATTTC TGCTTTACTA ACGTGTTTAC AACTATCATG 
ATCCCCTACC ACATCCTGAT TGTCCCTAGC AAGAAGCTCG GCGGCTCCAT GTTCACAGCC 
AACCCCTGGA TCTGTATATC TGGGGAGCTG GGAGAGACTG GGGTACTGCA GGTTCCCAGG 
AATATTCTAG AGATGACGTA TGAGTGCCAG AACCTCGGGA AGCTGACGAC TGTACAGATT 
GGACACGACA ATTCTGGCCT GTATGCCAAG TGGCTGGTGG AATATGTTAT GGTCCGAAAT 
GAAATAACTG GACACACCTA CAAGTTCCCT TGTGGGCGCT GGTTGGGAAA AGGAATGGAT 
GATGGCAGTC TGGAACGTGT GCTGGTTGGA GACCTGCTTA CATCCAGTAC AGACACTGAA 
GAGAAACCAT GCCGGACCCC TCCCCTGCAG CACTCACCCA GCATGATCAG GAGGCTTGTG 
GCCATATCCC CCAATAGCCG ACCAAAATTA AATACGGGTC AGATCCAGGA GGCTATTGGA 
GAAGCAGTGA ATGGAATTGT GAAACATTTT CATAAACCAG AGAAAGAGAG AGGCAGTTTA 
ACGTTATTAC TGTGCGGAGA GAACGGACTG GTGTCTGCCC TTGAACAAGT TTTCCAGCAT 
GGAATCCGAT CCCCTCGGCT TTTCAAAAGC ATCTTCATCT GGGACTTCTT AGAAAAAGCA 
CAAGCTTATT ACGAATCTCT GGAAGTGAAT GAATTGGTGC CAGAAGAGGA CTGGCAGAAA 
CGGGCGCGGA ATTTCTCCCG CTTTATTAAC GCCATTAACA ACAACCCTAG AAACATTGGG 
AAAGACGGCA AGTTTCAGAT GTTTGTTTGC TTAGGAGCCC GGGACCACCA TTTGCACCAC 
TGGATTGCAC TGCTGGCCGA CTGCCCCATT ATAGCACAAA TGTACGAGGA GACGGCTCTA 
ATCAAGGACC ACACGTTGGT GAACTCTCTA ATCCGAGTGT TACAGACACT ACAAGAGTTT 
AACATCACCT TAGAAAGCTC CCTCATTAAA GGAATCAATA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_031899880.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755740.1 Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.

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CloneID	OXa55810
Clone ID Related Accession (Same CDS sequence)	XM_031899880.1
Accession Version	XM_031899880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGCG GGAGAGGAGG GCGGTTTGAC AGCAGCACCA TGAGCGGGGG CGGGGGAGGC 
AGCAGCGCGG CCCCGGGCCG TTTCGCTGAT TATTTCGTGA TCTGTGGGCT GGATACGGGC 
ACCGGGCTGG AGCCGGACGA GTTGTCTGCA TTGTGTGAGT ACATACAGGC TTCTAAAGCC 
AGAGACGGGG CTGGACGTTT CTTACCCAGT GCGGCTGAAG GAGAGAATTT TGAGCAGACC 
CCTCTGCGCC GCACCTTTAA ATCCAAGGTC TTGGCGCGGT ACCCAGAGAA CGTGGAGTGG 
AACGCCTTCG ACCAGGATGC CGTGGGCATG CTATGCATGC CTAAAGGATT AGCATTTAAG 
ACTCAGGCCG ACCCCAAGGA GCCCCAGTAC CACGCGTTCA TCATCACGAG AGAAGACGGC 
TCTAGAACAT TTGGGTTCTC CCTCACCTTC TTTGAAGAAG TCACCAGCAA GCAGATATGC 
AGCGCCATGC AGACCCTGTA CCACATGCAT AACGCCGAGT ATGACATCAT CCACACGTCG 
GCGAGCAACG GCAGAGACTG CCCCGGAGAG TGCACCGACA GCTCCATCAC CAAGCTGCAG 
CGCTTCAACT CTTATGACAT AACACGAGAC ACCCTGTATG TGTCCAAGTG CATCTGTCTC 
ATCGCCCCCA TGTCTTTCAT GACCGCCTGT AAGAAAGTCC TGGAGCAGCT CCACCAGGCT 
GTGACTTCCC CACAGCCGCC GCCATTACCC TTAGAAAGTT ACATTTATAA CATCCTCTAT 
GAGGTGCCTC TGCCCCCGGC TGGGAGGTCC CTGAAGTTTA ATGGGGTGTA TGGACCAATC 
GTGTGTCAGA GGCCGAGCAC CAATGAGCTG CCCTTGTTTG ACTTTCCTGC CCAGGAAGTG 
TTTGAGTTGC TTGGGGTGGA AAACGTGGTG CAGCTGTTTA CGTGCGCCCT GCTGGAATTC 
CAGATATTGC TCTACTCCCA ACATTACCAG AGGTTAATGA CGGTGGCAGA GACCATCACA 
GGCCTGATGT TCCCATTTCA GTGGCAGCAT GTCTACGTCC CCATCTTGCC AGCCTCCCTC 
CTCCATTTCC TGGACGCGCC GGTTCCGTAC CTAATGGGAT TGCACTCCAA TGGGCTGGAT 
GACAGATCTA AGCTGGAGCT GCCACAAGAG GCCAACCTGT GCTTCGTGGA CATAGACAAC 
CACTTTATAG AGCTGCCCGA GGACCTGCCG CAGTTCCCCA ACAAGCTGGA ATTCATACAG 
GAGGTATCGG AGATCCTTTT GGCCTTCGGC ATCCCCCCCG AGGGGAACCT GCACTGCAGC 
GAGAGCATCT CCAAGCTGAG GAACCTCAAG GCAGCCGACC TGGTGTCCGA CAAGAGGAAC 
GGAAACCTGG CCGGCTCCCC ATTAAACTCC TATGAACTTT TAAAAGAGAA CGAGACTATT 
GCCCGGCTTC AGGCGCTGGT GAAGAGGACG GGGGTCAGTC TGGAAAAACA GGTGGAAGTG 
CGGGAGGAGT CGAGCAGCAA AAAGGACGGC AAAGCTCACT GTGACGAGGA GGAGCTCCGA 
ATGCACCAAC TCAACATCCA GGTCCGGGAA GTCTTTGCCA ACCGCTTCAC TCAGATGTTT 
GCCGATTATG AGGTGTTTGT GATCCAGCCC AGCCAGGACA AGGAATCCTG GTTCACTAAC 
AGGGAGCAAA TGCAGAATTT TGATAAAGCC TCGTTCCTAT CAGACCAGCC GGAGCCCTAC 
CTCCCCTTCC TCTCCCGGTT CCTCGAGACA CAGATGTTTG CTTCCTTCAT CGACAATAAA 
ATCATGTGCC ACGATGACGA TGACAAGGAT CCAGTTTTAC GGGTATTTGA TTCTCGGGTG 
GATAAAATTC GCCAGCTAAA CGTCCGGACC CCCACTCTCC GCACATCCAT GTACCAGAAG 
TGCACCGCCA TCGAGGAGGC CGTAAAAGCC ATAGAGCAAA GACTGTCCAA AATAGACCAC 
ACGGCGATCC ACCCCCATCT GCTGGATATG AAGATAGGGC AGGGAAAGTA CGAGCCTGGA 
TTCTTCCCCA AACTGCAGTC GGATGTTCTT TCCACGGGAC CTGCCACAAA TAAATGGACC 
AAGAGGAACG CGCCAGCACA GTGGAGGAGG AAGGACAGAC AGAAGCAGCA CTCAGAGCAC 
CTTAGGCTGG ACAATGACCA GAGAGAGAAA TACATTCAAG AAGCAAGAAA TATGAGCAGC 
GGGATCCGGC AACCCAAACT GTCCAACCTG TCACCCTCTG TAATTGCGCA GACCAACTGG 
AAGTTTGTGG AGGGGCTGCT GAAGGAATGT CGGAACAAGA CCAAGAGAAT GCTGGTGGAG 
AAGATGGGAA GGGAAGCGGT AGAGCTGGGC CACGGAGAAG TGAGCATTAC AGGGGTAGAA 
GAGAATGCTC TGATCGCCAG TCTGTGTGAC CTGCTGGAGA GAATTTGGAG CCACGGTCTG 
CAAGTGAAGC AGGGGAAGTC AGCCTTGTGG TCACACTTAC TGCATTACCA GGACAGCAGG 
AGGAGAGCGA CCTGGGGCAG TCTTGGCGGA ACAGATTTGG TACTGGACTG TGAGAGAAGG 
AAGTCTGACC CCTTTCTGGT CATGTACCCC CTAAAGATCT CACTTGTTCA GGATATGAGG 
CATATTCAGA ACATTGGAGA AATCAAGACA GATGTAGGGA AGGCGCGAGC CTGGGTCAGG 
CTGTCTATGG AAAAGAAACT TCTGTCCCGG CACCTGAAGC AGCTGCTGTT GGATCATGAT 
CTCAGCAAGA AGCTGTACAA GCGCTACGCC TTCCTGCGCT GCGACGATGA GAAGGAACAG 
TTCCTCTATC ATTTGCTCTC CTTCAATGCT GTGGATTATT TCTGCTTTAC TAACGTGTTT 
ACAACTATCA TGATCCCCTA CCACATCCTG ATTGTCCCTA GCAAGAAGCT CGGCGGCTCC 
ATGTTCACAG CCAACCCCTG GATCTGTATA TCTGGGGAGC TGGGAGAGAC TGGGGTACTG 
CAGGTTCCCA GGAATATTCT AGAGATGACG TATGAGTGCC AGAACCTCGG GAAGCTGACG 
ACTGTACAGA TTGGACACGA CAATTCTGGC CTGTATGCCA AGTGGCTGGT GGAATATGTT 
ATGGTCCGAA ATGAAATAAC TGGACACACC TACAAGTTCC CTTGTGGGCG CTGGTTGGGA 
AAAGGAATGG ATGATGGCAG TCTGGAACGT GTGCTGGTTG GAGACCTGCT TACATCCAGT 
ACAGACACTG AAGAGAAACC ATGCCGGACC CCTCCCCTGC AGCACTCACC CAGCATGATC 
AGGAGGCTTG TGGCCATATC CCCCAATAGC CGACCAAAAT TAAATACGGG TCAGATCCAG 
GAGGCTATTG GAGAAGCAGT GAATGGAATT GTGAAACATT TTCATAAACC AGAGAAAGAG 
AGAGGCAGTT TAACGTTATT ACTGTGCGGA GAGAACGGAC TGGTGTCTGC CCTTGAACAA 
GTTTTCCAGC ATGGAATCCG ATCCCCTCGG CTTTTCAAAA GCATCTTCAT CTGGGACTTC 
TTAGGGACCA CCATTTGCAC CACTGGATTG CACTGCTGGC CGACTGCCCC ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755740.1
CDS	129..3725
Translation	MTGGRGGRFDSSTMSGGGGGSSAAPGRFADYFVICGLDTGTGLEPDELSALCEYIQASKARDGAGRFLPSAAEGENFEQTPLRRTFKSKVLARYPENVEWNAFDQDAVGMLCMPKGLAFKTQADPKEPQYHAFIITREDGSRTFGFSLTFFEEVTSKQICSAMQTLYHMHNAEYDIIHTSASNGRDCPGECTDSSITKLQRFNSYDITRDTLYVSKCICLIAPMSFMTACKKVLEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPAGRSLKFNGVYGPIVCQRPSTNELPLFDFPAQEVFELLGVENVVQLFTCALLEFQILLYSQHYQRLMTVAETITGLMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEVSEILLAFGIPPEGNLHCSESISKLRNLKAADLVSDKRNGNLAGSPLNSYELLKENETIARLQALVKRTGVSLEKQVEVREESSSKKDGKAHCDEEELRMHQLNIQVREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDNKIMCHDDDDKDPVLRVFDSRVDKIRQLNVRTPTLRTSMYQKCTAIEEAVKAIEQRLSKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQSDVLSTGPATNKWTKRNAPAQWRRKDRQKQHSEHLRLDNDQREKYIQEARNMSSGIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENALIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQDSRRRATWGSLGGTDLVLDCERRKSDPFLVMYPLKISLVQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLLDHDLSKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETGVLQVPRNILEMTYECQNLGKLTTVQIGHDNSGLYAKWLVEYVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGDLLTSSTDTEEKPCRTPPLQHSPSMIRRLVAISPNSRPKLNTGQIQEAIGEAVNGIVKHFHKPEKERGSLTLLLCGENGLVSALEQVFQHGIRSPRLFKSIFIWDFLGTTICTTGLHCWPTAPL

Target ORF information:

RefSeq Version	XM_031899880.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899880.1

ORF Insert Sequence:

ATGACAGGCG GGAGAGGAGG GCGGTTTGAC AGCAGCACCA TGAGCGGGGG CGGGGGAGGC 
AGCAGCGCGG CCCCGGGCCG TTTCGCTGAT TATTTCGTGA TCTGTGGGCT GGATACGGGC 
ACCGGGCTGG AGCCGGACGA GTTGTCTGCA TTGTGTGAGT ACATACAGGC TTCTAAAGCC 
AGAGACGGGG CTGGACGTTT CTTACCCAGT GCGGCTGAAG GAGAGAATTT TGAGCAGACC 
CCTCTGCGCC GCACCTTTAA ATCCAAGGTC TTGGCGCGGT ACCCAGAGAA CGTGGAGTGG 
AACGCCTTCG ACCAGGATGC CGTGGGCATG CTATGCATGC CTAAAGGATT AGCATTTAAG 
ACTCAGGCCG ACCCCAAGGA GCCCCAGTAC CACGCGTTCA TCATCACGAG AGAAGACGGC 
TCTAGAACAT TTGGGTTCTC CCTCACCTTC TTTGAAGAAG TCACCAGCAA GCAGATATGC 
AGCGCCATGC AGACCCTGTA CCACATGCAT AACGCCGAGT ATGACATCAT CCACACGTCG 
GCGAGCAACG GCAGAGACTG CCCCGGAGAG TGCACCGACA GCTCCATCAC CAAGCTGCAG 
CGCTTCAACT CTTATGACAT AACACGAGAC ACCCTGTATG TGTCCAAGTG CATCTGTCTC 
ATCGCCCCCA TGTCTTTCAT GACCGCCTGT AAGAAAGTCC TGGAGCAGCT CCACCAGGCT 
GTGACTTCCC CACAGCCGCC GCCATTACCC TTAGAAAGTT ACATTTATAA CATCCTCTAT 
GAGGTGCCTC TGCCCCCGGC TGGGAGGTCC CTGAAGTTTA ATGGGGTGTA TGGACCAATC 
GTGTGTCAGA GGCCGAGCAC CAATGAGCTG CCCTTGTTTG ACTTTCCTGC CCAGGAAGTG 
TTTGAGTTGC TTGGGGTGGA AAACGTGGTG CAGCTGTTTA CGTGCGCCCT GCTGGAATTC 
CAGATATTGC TCTACTCCCA ACATTACCAG AGGTTAATGA CGGTGGCAGA GACCATCACA 
GGCCTGATGT TCCCATTTCA GTGGCAGCAT GTCTACGTCC CCATCTTGCC AGCCTCCCTC 
CTCCATTTCC TGGACGCGCC GGTTCCGTAC CTAATGGGAT TGCACTCCAA TGGGCTGGAT 
GACAGATCTA AGCTGGAGCT GCCACAAGAG GCCAACCTGT GCTTCGTGGA CATAGACAAC 
CACTTTATAG AGCTGCCCGA GGACCTGCCG CAGTTCCCCA ACAAGCTGGA ATTCATACAG 
GAGGTATCGG AGATCCTTTT GGCCTTCGGC ATCCCCCCCG AGGGGAACCT GCACTGCAGC 
GAGAGCATCT CCAAGCTGAG GAACCTCAAG GCAGCCGACC TGGTGTCCGA CAAGAGGAAC 
GGAAACCTGG CCGGCTCCCC ATTAAACTCC TATGAACTTT TAAAAGAGAA CGAGACTATT 
GCCCGGCTTC AGGCGCTGGT GAAGAGGACG GGGGTCAGTC TGGAAAAACA GGTGGAAGTG 
CGGGAGGAGT CGAGCAGCAA AAAGGACGGC AAAGCTCACT GTGACGAGGA GGAGCTCCGA 
ATGCACCAAC TCAACATCCA GGTCCGGGAA GTCTTTGCCA ACCGCTTCAC TCAGATGTTT 
GCCGATTATG AGGTGTTTGT GATCCAGCCC AGCCAGGACA AGGAATCCTG GTTCACTAAC 
AGGGAGCAAA TGCAGAATTT TGATAAAGCC TCGTTCCTAT CAGACCAGCC GGAGCCCTAC 
CTCCCCTTCC TCTCCCGGTT CCTCGAGACA CAGATGTTTG CTTCCTTCAT CGACAATAAA 
ATCATGTGCC ACGATGACGA TGACAAGGAT CCAGTTTTAC GGGTATTTGA TTCTCGGGTG 
GATAAAATTC GCCAGCTAAA CGTCCGGACC CCCACTCTCC GCACATCCAT GTACCAGAAG 
TGCACCGCCA TCGAGGAGGC CGTAAAAGCC ATAGAGCAAA GACTGTCCAA AATAGACCAC 
ACGGCGATCC ACCCCCATCT GCTGGATATG AAGATAGGGC AGGGAAAGTA CGAGCCTGGA 
TTCTTCCCCA AACTGCAGTC GGATGTTCTT TCCACGGGAC CTGCCACAAA TAAATGGACC 
AAGAGGAACG CGCCAGCACA GTGGAGGAGG AAGGACAGAC AGAAGCAGCA CTCAGAGCAC 
CTTAGGCTGG ACAATGACCA GAGAGAGAAA TACATTCAAG AAGCAAGAAA TATGAGCAGC 
GGGATCCGGC AACCCAAACT GTCCAACCTG TCACCCTCTG TAATTGCGCA GACCAACTGG 
AAGTTTGTGG AGGGGCTGCT GAAGGAATGT CGGAACAAGA CCAAGAGAAT GCTGGTGGAG 
AAGATGGGAA GGGAAGCGGT AGAGCTGGGC CACGGAGAAG TGAGCATTAC AGGGGTAGAA 
GAGAATGCTC TGATCGCCAG TCTGTGTGAC CTGCTGGAGA GAATTTGGAG CCACGGTCTG 
CAAGTGAAGC AGGGGAAGTC AGCCTTGTGG TCACACTTAC TGCATTACCA GGACAGCAGG 
AGGAGAGCGA CCTGGGGCAG TCTTGGCGGA ACAGATTTGG TACTGGACTG TGAGAGAAGG 
AAGTCTGACC CCTTTCTGGT CATGTACCCC CTAAAGATCT CACTTGTTCA GGATATGAGG 
CATATTCAGA ACATTGGAGA AATCAAGACA GATGTAGGGA AGGCGCGAGC CTGGGTCAGG 
CTGTCTATGG AAAAGAAACT TCTGTCCCGG CACCTGAAGC AGCTGCTGTT GGATCATGAT 
CTCAGCAAGA AGCTGTACAA GCGCTACGCC TTCCTGCGCT GCGACGATGA GAAGGAACAG 
TTCCTCTATC ATTTGCTCTC CTTCAATGCT GTGGATTATT TCTGCTTTAC TAACGTGTTT 
ACAACTATCA TGATCCCCTA CCACATCCTG ATTGTCCCTA GCAAGAAGCT CGGCGGCTCC 
ATGTTCACAG CCAACCCCTG GATCTGTATA TCTGGGGAGC TGGGAGAGAC TGGGGTACTG 
CAGGTTCCCA GGAATATTCT AGAGATGACG TATGAGTGCC AGAACCTCGG GAAGCTGACG 
ACTGTACAGA TTGGACACGA CAATTCTGGC CTGTATGCCA AGTGGCTGGT GGAATATGTT 
ATGGTCCGAA ATGAAATAAC TGGACACACC TACAAGTTCC CTTGTGGGCG CTGGTTGGGA 
AAAGGAATGG ATGATGGCAG TCTGGAACGT GTGCTGGTTG GAGACCTGCT TACATCCAGT 
ACAGACACTG AAGAGAAACC ATGCCGGACC CCTCCCCTGC AGCACTCACC CAGCATGATC 
AGGAGGCTTG TGGCCATATC CCCCAATAGC CGACCAAAAT TAAATACGGG TCAGATCCAG 
GAGGCTATTG GAGAAGCAGT GAATGGAATT GTGAAACATT TTCATAAACC AGAGAAAGAG 
AGAGGCAGTT TAACGTTATT ACTGTGCGGA GAGAACGGAC TGGTGTCTGC CCTTGAACAA 
GTTTTCCAGC ATGGAATCCG ATCCCCTCGG CTTTTCAAAA GCATCTTCAT CTGGGACTTC 
TTAGGGACCA CCATTTGCAC CACTGGATTG CACTGCTGGC CGACTGCCCC ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( NM_001127996.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001121468.1 Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), mRNA.

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CloneID	OXa03116
Clone ID Related Accession (Same CDS sequence)	NM_001127996.1
Accession Version	NM_001127996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166235.1. ##Evidence-Data-START## Transcript exon combination :: BC166235.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGAGCGGGG GCGGGGGAGG CAGCAGCACG GCCCCGGGCC GTTTCGCCGA TTATTTCGTG 
ATCTGTGGGC TGGATACGGG CACCGGGCTG GAGCCGGACG AGTTGTCTGC ATTGTGTGAG 
TACATACAGG CTTCTAAAGC CAGAGACGGG GCTGGACGTT TCTTACCCAG TGCGGCTGAA 
GGAGAGAATT TTGAGCAGAC CCCTCTGCGC CGCACCTTTA AATCCAAGGT CTTGGCGCGG 
TACCCAGAGA ACGTGGAGTG GAACGCCTTC GACCAGGATG CCGTGGGCAT GCTATGCATG 
CCTAAAGGAT TAGCATTTAA GACTCAGGCC GACCCCAAGG AGCCCCAGTA CCACGCGTTC 
ATCATCACGA GAGAAGACGG CTCTAGAACA TTTGGGTTCT CCCTCACCTT CTTTGAAGAA 
GTCACCAGCA AGCAGATATG CAGCGCCATG CAGACCCTGT ACCACATGCA TAACGCCGAG 
TATGACATCA TCCACACGTC GGCGAGCAAC GGCAGAGACT GCCCCGGAGA GTGCACCGAC 
AGCTCCATCA CCAAGCTGCA GCGCTTCAAC TCTTATGACA TAACACGAGA CACCCTGTAT 
GTGTCCAAGT GCATCTGTCT CATCGCCCCC ATGTCTTTCA TGACCGCCTG TAAGAAAGTC 
CTGGAGCAGC TCCACCAGGC TGTGACTTCC CCACAGCCGC CGCCATTACC CTTAGAAAGT 
TACATTTATA ACATCCTCTA TGAGGTGCCT CTGCCCCCGG CTGGGAGGTC CCTGAAGTTT 
AATGGGGTGT ATGGACCAAT CGTGTGTCAG AGGCCGAGCA CCAATGAGCT GCCCTTGTTT 
GACTTTCCTG CCCAGGAAGT GTTTGAGTTG CTTGGGGTGG AAAACGTGGT GCAGCTGTTT 
ACGTGCGCCC TGCTGGAATT CCAGATATTG CTCTACTCCC AACATTACCA GAGGTTAATG 
ACGGTGGCAG AGACCATCAC AGGCCTGATG TTCCCATTTC AGTGGCAGCA TGTCTACGTC 
CCCATCTTGC CAGCCTCCCT CCTCCATTTC CTGGACGCGC CGGTTCCGTA CCTAATGGGA 
TTGCACTCCA ATGGGCTGGA TGACAGATCT AAGCTGGAGC TGCCACAAGA GGCCAACCTG 
TGCTTCGTGG ACATAGACAA CCACTTTATA GAGCTGCCCG AGGACCTGCC GCAGTTCCCC 
AACAAGCTGG AATTCATACA GGAGGTATCG GAGATCCTTT TGGCCTTCGG CATCCCCCCC 
GAGGGGAACC TGCACTGCAG CGAGAGCATC TCCAAGCTGA GGAACCTCAA GGCAGCCGAC 
CTGGTGTCCG ACAAGAGGAA CGGAAACCTG GCCGGCTCCC CATTAAACTC CTATGAACTT 
TTAAAAGAGA ACGAGACTAT TGCCCGGCTT CAGGCGCTGG TGAAGAGGAC GGGGGTCAGT 
CTGGAAAAAC AGGTGGAAGT GCGGGAGGAG TCGAGCAGCA AAAAGGACGG CAAAGCTCAC 
TGTGACGAGG AGGAGCTCCG AATGCACCAA CTCAACATCC AGGTCCGGGA AGTCTTTGCC 
AACCGCTTCA CTCAGATGTT TGCCGATTAT GAGGTGTTTG TGATCCAGCC CAGCCAGGAC 
AAGGAATCCT GGTTCACTAA CAGGGAGCAA ATGCAGAATT TTGATAAAGC CTCGTTCCTA 
TCAGACCAGC CGGAACCCTA CCTCCCCTTC CTCTCCCGGT TCCTGGAGAC ACAGATGTTT 
GCTTCCTTCA TCGACAATAA AATCATGTGC CACGACGACG ATGACAAGGA TCCAGTTTTA 
CGGGTATTCG ATTCTCGGGT GGATAAAATT CGCCAGCTAA ACGTCCGGAC CCCCACTCTC 
CGCACATCCA TGTACCAGAA GTGCACCGCC ATCGAGGAGG CCGTAAAAGC CATAGAGCAA 
AGACTGTCCA AAATAGACCA CACGGCGATC CACCCCCATC TGCTGGATAT GAAGATAGGG 
CAGGGAAAGT ACGAGCCTGG ATTCTTCCCC AAACTGCAGT CGGATGTTCT TTCCACGGGA 
CCTGCCACAA ATAAATGGAC CAAGAGGAAC GCGCCAGCAC AGTGGAGGAG GAAGGACAGA 
CAGAAGCAGC ACTCAGAGCA CCTTAGGCTG GACAATGACC AGAGAGAGAA ATACATTCAA 
GAAGCAAGAA ATATGAGCAG CGGGATCCGG CAACCCAAAC TGTCCAACCT GTCACCCTCT 
GTAATTGCGC AGACCAACTG GAAGTTTGTG GAGGGGCTGC TGAAGGAATG TCGGAACAAG 
ACCAAGAGAA TGCTGGTGGA GAAGATGGGA AGGGAAGCGG TAGAGCTGGG CCACGGAGAA 
GTGAGCATTA CAGGGGTAGA AGAGAATGCT CTGATCGCCA GTCTGTGTGA CCTGCTGGAG 
AGAATTTGGA GCCACGGTCT GCAAGTGAAG CAGGGGAAGT CAGCCTTGTG GTCACACTTA 
CTGCATTACC AGGACAGCAG GAGGAGAGCG ACCTGGGGCA GTCTTGGCGG AACAGATTTG 
GTACTGGACT GTGAGAGAAG GAAGTCTGAC CCCTTTCTGG TCATGTACCC CCTAAAGATC 
TCACTTGTTC AGGATATGAG GCATATTCAG AACATTGGAG AAATCAAGAC AGATGTAGGG 
AAGGCGCGAG CCTGGGTCAG GCTGTCTATG GAAAAGAAAC TTCTGTCCCG GCACCTGAAG 
CAGCTGCTGT TGGATCATGA TCTCAGCAAG AAGCTGTACA AGCGCTACGC CTTCCTGCGC 
TGCGACGATG AGAAGGAACA GTTCCTCTAT CATTTGCTCT CCTTCAATGC TGTGGATTAT 
TTCTGCTTTA CTAACGTGTT TACAACTATC ATGATCCCCT ACCACATCCT GATTGTCCCT 
AGCAAGAAGC TCGGCGGCTC CATGTTCACA GCCAACCCCT GGATCTGTAT ATCTGGGGAG 
CTGGGAGAGA CTGGGGTACT GCAGGTTCCC AGGAATATTC TAGAGATGAC GTATGAGTGC 
CAGAACCTCG GGAAGCTGAC GACTGTACAG ATTGGACACG ACAATTCTGG CCTGTATGCC 
AAGTGGCTGG TGGAATATGT TATGGTCCGA AATGAAATAA CTGGACACAC CTACAAGTTC 
CCTTGTGGGC GCTGGTTGGG AAAAGGAATG GATGATGGCA GTCTGGAACG TGTGCTGGTT 
GGAGACCTGC TTACATCCAG TACAGACACT GAAGAGAAAC CATGCCGGAC CCCTCCCCTG 
CAGCACTCAC CCAGCATGAT CAGGAGGCTT GTGGCCATAT CCCCCAATAG CCGACCAAAA 
TTAAATACGG GTCAGATCCA GGAGGCTATT GGAGAAGCAG TGAATGGAAT TGTGAAACAT 
TTTCATAAAC CAGAGAAAGA GAGAGGCAGT TTAACGTTAT TACTGTGCGG AGAGAACGGA 
CTGGTGTCTG CCCTTGAACA AGTTTTCCAG CATGGAATCC GATCCCCTCG GCTTTTCAAA 
AGCATCTTCA TCTGGGACTT CTTAGAAAAA GCACAAGCTT ATTACGAATC TCTGGAAGTG 
AATGAATTGG TGCCAGAAGA GGACTGGCAG AAACGGGCGC GGAATTTCTC CCGCTTTATT 
AACGCCATTA ACAACAACCC TAGAAACATT GGGAAAGACG GCAAGTTTCA GATGTTTGTT 
TGCTTAGGAG CCCGGGACCA CCATTTGCAC CACTGGATTG CACTGCTGGC CGACTGCCCC 
ATTATAGCAC AAATGTACGA GGAGACGGCT CTAATCAAGG ACCACACGTT GGTGAACTCT 
CTAATCCGAG TGTTACAGAC ACTACAAGAG TTTAACATCA CCTTAGAAAG CTCCCTCATT 
AAAGGAATCA ATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121468.1
CDS	112..3969
Translation	MSGGGGGSSTAPGRFADYFVICGLDTGTGLEPDELSALCEYIQASKARDGAGRFLPSAAEGENFEQTPLRRTFKSKVLARYPENVEWNAFDQDAVGMLCMPKGLAFKTQADPKEPQYHAFIITREDGSRTFGFSLTFFEEVTSKQICSAMQTLYHMHNAEYDIIHTSASNGRDCPGECTDSSITKLQRFNSYDITRDTLYVSKCICLIAPMSFMTACKKVLEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPAGRSLKFNGVYGPIVCQRPSTNELPLFDFPAQEVFELLGVENVVQLFTCALLEFQILLYSQHYQRLMTVAETITGLMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEVSEILLAFGIPPEGNLHCSESISKLRNLKAADLVSDKRNGNLAGSPLNSYELLKENETIARLQALVKRTGVSLEKQVEVREESSSKKDGKAHCDEEELRMHQLNIQVREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDNKIMCHDDDDKDPVLRVFDSRVDKIRQLNVRTPTLRTSMYQKCTAIEEAVKAIEQRLSKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQSDVLSTGPATNKWTKRNAPAQWRRKDRQKQHSEHLRLDNDQREKYIQEARNMSSGIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENALIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQDSRRRATWGSLGGTDLVLDCERRKSDPFLVMYPLKISLVQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLLDHDLSKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETGVLQVPRNILEMTYECQNLGKLTTVQIGHDNSGLYAKWLVEYVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGDLLTSSTDTEEKPCRTPPLQHSPSMIRRLVAISPNSRPKLNTGQIQEAIGEAVNGIVKHFHKPEKERGSLTLLLCGENGLVSALEQVFQHGIRSPRLFKSIFIWDFLEKAQAYYESLEVNELVPEEDWQKRARNFSRFINAINNNPRNIGKDGKFQMFVCLGARDHHLHHWIALLADCPIIAQMYEETALIKDHTLVNSLIRVLQTLQEFNITLESSLIKGINI

Target ORF information:

RefSeq Version	NM_001127996.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5A (dennd5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127996.1

ORF Insert Sequence:

ATGAGCGGGG GCGGGGGAGG CAGCAGCACG GCCCCGGGCC GTTTCGCCGA TTATTTCGTG 
ATCTGTGGGC TGGATACGGG CACCGGGCTG GAGCCGGACG AGTTGTCTGC ATTGTGTGAG 
TACATACAGG CTTCTAAAGC CAGAGACGGG GCTGGACGTT TCTTACCCAG TGCGGCTGAA 
GGAGAGAATT TTGAGCAGAC CCCTCTGCGC CGCACCTTTA AATCCAAGGT CTTGGCGCGG 
TACCCAGAGA ACGTGGAGTG GAACGCCTTC GACCAGGATG CCGTGGGCAT GCTATGCATG 
CCTAAAGGAT TAGCATTTAA GACTCAGGCC GACCCCAAGG AGCCCCAGTA CCACGCGTTC 
ATCATCACGA GAGAAGACGG CTCTAGAACA TTTGGGTTCT CCCTCACCTT CTTTGAAGAA 
GTCACCAGCA AGCAGATATG CAGCGCCATG CAGACCCTGT ACCACATGCA TAACGCCGAG 
TATGACATCA TCCACACGTC GGCGAGCAAC GGCAGAGACT GCCCCGGAGA GTGCACCGAC 
AGCTCCATCA CCAAGCTGCA GCGCTTCAAC TCTTATGACA TAACACGAGA CACCCTGTAT 
GTGTCCAAGT GCATCTGTCT CATCGCCCCC ATGTCTTTCA TGACCGCCTG TAAGAAAGTC 
CTGGAGCAGC TCCACCAGGC TGTGACTTCC CCACAGCCGC CGCCATTACC CTTAGAAAGT 
TACATTTATA ACATCCTCTA TGAGGTGCCT CTGCCCCCGG CTGGGAGGTC CCTGAAGTTT 
AATGGGGTGT ATGGACCAAT CGTGTGTCAG AGGCCGAGCA CCAATGAGCT GCCCTTGTTT 
GACTTTCCTG CCCAGGAAGT GTTTGAGTTG CTTGGGGTGG AAAACGTGGT GCAGCTGTTT 
ACGTGCGCCC TGCTGGAATT CCAGATATTG CTCTACTCCC AACATTACCA GAGGTTAATG 
ACGGTGGCAG AGACCATCAC AGGCCTGATG TTCCCATTTC AGTGGCAGCA TGTCTACGTC 
CCCATCTTGC CAGCCTCCCT CCTCCATTTC CTGGACGCGC CGGTTCCGTA CCTAATGGGA 
TTGCACTCCA ATGGGCTGGA TGACAGATCT AAGCTGGAGC TGCCACAAGA GGCCAACCTG 
TGCTTCGTGG ACATAGACAA CCACTTTATA GAGCTGCCCG AGGACCTGCC GCAGTTCCCC 
AACAAGCTGG AATTCATACA GGAGGTATCG GAGATCCTTT TGGCCTTCGG CATCCCCCCC 
GAGGGGAACC TGCACTGCAG CGAGAGCATC TCCAAGCTGA GGAACCTCAA GGCAGCCGAC 
CTGGTGTCCG ACAAGAGGAA CGGAAACCTG GCCGGCTCCC CATTAAACTC CTATGAACTT 
TTAAAAGAGA ACGAGACTAT TGCCCGGCTT CAGGCGCTGG TGAAGAGGAC GGGGGTCAGT 
CTGGAAAAAC AGGTGGAAGT GCGGGAGGAG TCGAGCAGCA AAAAGGACGG CAAAGCTCAC 
TGTGACGAGG AGGAGCTCCG AATGCACCAA CTCAACATCC AGGTCCGGGA AGTCTTTGCC 
AACCGCTTCA CTCAGATGTT TGCCGATTAT GAGGTGTTTG TGATCCAGCC CAGCCAGGAC 
AAGGAATCCT GGTTCACTAA CAGGGAGCAA ATGCAGAATT TTGATAAAGC CTCGTTCCTA 
TCAGACCAGC CGGAACCCTA CCTCCCCTTC CTCTCCCGGT TCCTGGAGAC ACAGATGTTT 
GCTTCCTTCA TCGACAATAA AATCATGTGC CACGACGACG ATGACAAGGA TCCAGTTTTA 
CGGGTATTCG ATTCTCGGGT GGATAAAATT CGCCAGCTAA ACGTCCGGAC CCCCACTCTC 
CGCACATCCA TGTACCAGAA GTGCACCGCC ATCGAGGAGG CCGTAAAAGC CATAGAGCAA 
AGACTGTCCA AAATAGACCA CACGGCGATC CACCCCCATC TGCTGGATAT GAAGATAGGG 
CAGGGAAAGT ACGAGCCTGG ATTCTTCCCC AAACTGCAGT CGGATGTTCT TTCCACGGGA 
CCTGCCACAA ATAAATGGAC CAAGAGGAAC GCGCCAGCAC AGTGGAGGAG GAAGGACAGA 
CAGAAGCAGC ACTCAGAGCA CCTTAGGCTG GACAATGACC AGAGAGAGAA ATACATTCAA 
GAAGCAAGAA ATATGAGCAG CGGGATCCGG CAACCCAAAC TGTCCAACCT GTCACCCTCT 
GTAATTGCGC AGACCAACTG GAAGTTTGTG GAGGGGCTGC TGAAGGAATG TCGGAACAAG 
ACCAAGAGAA TGCTGGTGGA GAAGATGGGA AGGGAAGCGG TAGAGCTGGG CCACGGAGAA 
GTGAGCATTA CAGGGGTAGA AGAGAATGCT CTGATCGCCA GTCTGTGTGA CCTGCTGGAG 
AGAATTTGGA GCCACGGTCT GCAAGTGAAG CAGGGGAAGT CAGCCTTGTG GTCACACTTA 
CTGCATTACC AGGACAGCAG GAGGAGAGCG ACCTGGGGCA GTCTTGGCGG AACAGATTTG 
GTACTGGACT GTGAGAGAAG GAAGTCTGAC CCCTTTCTGG TCATGTACCC CCTAAAGATC 
TCACTTGTTC AGGATATGAG GCATATTCAG AACATTGGAG AAATCAAGAC AGATGTAGGG 
AAGGCGCGAG CCTGGGTCAG GCTGTCTATG GAAAAGAAAC TTCTGTCCCG GCACCTGAAG 
CAGCTGCTGT TGGATCATGA TCTCAGCAAG AAGCTGTACA AGCGCTACGC CTTCCTGCGC 
TGCGACGATG AGAAGGAACA GTTCCTCTAT CATTTGCTCT CCTTCAATGC TGTGGATTAT 
TTCTGCTTTA CTAACGTGTT TACAACTATC ATGATCCCCT ACCACATCCT GATTGTCCCT 
AGCAAGAAGC TCGGCGGCTC CATGTTCACA GCCAACCCCT GGATCTGTAT ATCTGGGGAG 
CTGGGAGAGA CTGGGGTACT GCAGGTTCCC AGGAATATTC TAGAGATGAC GTATGAGTGC 
CAGAACCTCG GGAAGCTGAC GACTGTACAG ATTGGACACG ACAATTCTGG CCTGTATGCC 
AAGTGGCTGG TGGAATATGT TATGGTCCGA AATGAAATAA CTGGACACAC CTACAAGTTC 
CCTTGTGGGC GCTGGTTGGG AAAAGGAATG GATGATGGCA GTCTGGAACG TGTGCTGGTT 
GGAGACCTGC TTACATCCAG TACAGACACT GAAGAGAAAC CATGCCGGAC CCCTCCCCTG 
CAGCACTCAC CCAGCATGAT CAGGAGGCTT GTGGCCATAT CCCCCAATAG CCGACCAAAA 
TTAAATACGG GTCAGATCCA GGAGGCTATT GGAGAAGCAG TGAATGGAAT TGTGAAACAT 
TTTCATAAAC CAGAGAAAGA GAGAGGCAGT TTAACGTTAT TACTGTGCGG AGAGAACGGA 
CTGGTGTCTG CCCTTGAACA AGTTTTCCAG CATGGAATCC GATCCCCTCG GCTTTTCAAA 
AGCATCTTCA TCTGGGACTT CTTAGAAAAA GCACAAGCTT ATTACGAATC TCTGGAAGTG 
AATGAATTGG TGCCAGAAGA GGACTGGCAG AAACGGGCGC GGAATTTCTC CCGCTTTATT 
AACGCCATTA ACAACAACCC TAGAAACATT GGGAAAGACG GCAAGTTTCA GATGTTTGTT 
TGCTTAGGAG CCCGGGACCA CCATTTGCAC CACTGGATTG CACTGCTGGC CGACTGCCCC 
ATTATAGCAC AAATGTACGA GGAGACGGCT CTAATCAAGG ACCACACGTT GGTGAACTCT 
CTAATCCGAG TGTTACAGAC ACTACAAGAG TTTAACATCA CCTTAGAAAG CTCCCTCATT 
AAAGGAATCA ATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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dennd5a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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