LOC100158554 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100158554
Entrez Gene ID	100158554
Full Name	epithelial chloride channel protein
Synonyms	clca3p
General protein information	Preferred Names epithelial chloride channel protein Names epithelial chloride channel protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012962454.2	XP_012817908.1	epithelial chloride channel protein
XM_002934158.4	XP_002934204.1	epithelial chloride channel protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC784768	NP_001229512.1
Danio rerio (zebrafish)	clca1	XP_002664610.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004059	XP_560062.4
Xenopus tropicalis (tropical clawed frog)	LOC100495107	XP_002931721.2
Canis lupus familiaris (dog)	LOC479964	XP_005622181.1
Bos taurus (cattle)	CLCA3	NP_851361.1
Mus musculus (house mouse)	Clca2	NP_085104.1
Rattus norvegicus (Norway rat)	Clca2	NP_001013220.1
Xenopus tropicalis (tropical clawed frog)	clca1	NP_001184129.1
Xenopus tropicalis (tropical clawed frog)	LOC100490932	XP_002934201.1
	LOC715292	XP_001105446.1
Mus musculus (house mouse)	Clca1	NP_034029.2
Xenopus tropicalis (tropical clawed frog)	LOC100488301	XP_002934186.1
Gallus gallus (chicken)	LOC424523	XP_422360.3
Xenopus tropicalis (tropical clawed frog)	clca3p	XP_002934204.1

Gene Ontology

The following LOC100158554 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100158554 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa09078	XM_012962454.2 Latest version!	Xenopus tropicalis epithelial chloride channel protein (LOC100158554), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa09078	XM_002934158.4 Latest version!	Xenopus tropicalis epithelial chloride channel protein (LOC100158554), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100158554 ( XM_012962454.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817908.1 Xenopus tropicalis epithelial chloride channel protein (LOC100158554), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa09078
Clone ID Related Accession (Same CDS sequence)	XM_012962454.2 , XM_002934158.4
Accession Version	XM_012962454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	epithelial chloride channel protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTCC TGCACATTCT AGCACTGGTT TCTGCTTTCC AGTCTGTACA TTTAGCAAGA 
GACTCATTGG TGCAGCTGAA AAACAATGGA TATGAAGATA TCATTATAGC AGTTAATCCC 
CAAGTGCCTG AAGATGGGAA GATTATTGAA AATATCAAGA AAATGCTGAC CGATGCATCT 
TCTTACTTGT TCCAAGCAAC TAAAAAAAGA CTTTATATAA GGAGTGCAAA AATTTTGATC 
CCCAATACTT GGGCAACCAA TAGTTCATAT GGTAGGCCGA AACTAGAGTC ATATGACAAG 
GCAGATGTGA TTGTTGCACC TCCTTTTGTG CAAGGGGATG ATCCATATAC CTTACAGTTT 
GGAGGTTGTG GAGAAAAGGG AAAATACATT CATTTCACTC CTAACTTCCT TGTAAATGAT 
GAGAAAATGC TACCAATATA TGGACCAAGA GGGAGAGTTT TTGTGCATGA GTGGGCTCAC 
TTTCGCTGGG GCGTTTTTGA CGAATACAAC TATAATCGCC CTTACTATTT TTCTGAAAAC 
CGGAAAGTTG AAGCAACAAG GTGCCCTTTG AAGTTGAAAG GCCTAAATCT AATAGATGTA 
TGCCAAAGGG GAGTTTGCAA TCTGGAACCT TGTGAATATG ACAAGAACAC AGGGCTCTAT 
GAAGAGGACT GCAAATTCTA TCCTGACAGA GATATCTTGG TGGAGGAATC TGTTATGTAT 
GCCCAAATGT TTGAACCTGT ACATGCCTTC TGTGATAGCA GTAGCCATAA TTCAGAGGCT 
CCCAACCAAC AAAACCGCCT GTGCAGCCAA CAGAGCACAT GGGATGTCAT CAGTAAGTCT 
TCCGACATAA AATCCAGTCC TCCTTTAAAG GACTCAAACA TTCCAGCTCC TGTGATTTCT 
CTATTGCAAT ACAAGGACAG AGTGGTCTCA TTGGTTCTGG ATATATCTGG AAGCATGACA 
AATGCTAACC GCATTACCCG GCTGTATCAA GCATCAGAAG TGTACATCAT GCAAATAGTG 
GAACAGGGTG CTTATGTTGG TATCGTTACT TTTAGCAATG TGGCCGAGAT CAAGTCACAA 
CTGGTTAAAA TAACGGATAC TTTTCAGCGG GAAAGTCTGA AGTTAAAGCT ACCAACTGTA 
GCGACTGGTG GTACAAACAT CTGTGCGGGA GTTCAACAAG GTTTGCAGGT AAATAGAAAT 
CTTGATCAGA GCACACATGG CACTGAAATT GTGCTGTTGA CAGATGGAGA AGATTCTGGA 
ATTAGCAGTT GTTTTCCTGA CATTACTAAG AGCGGAGCCA TTATCCACAC AATTGCACTT 
GGAAACAATG CAGACCCCGG GCTCGAAAAG CTTGCAGATC TCACAGGTGG CCTAAAACTG 
TATGCATCAG ATAAAGTAGA TGCAAATGGC CTGATTGATT CTTTCAGTGG GATTGTGTCC 
AACACTGGGA ATGTAACACA GCAATCCCTT CAGATTGAAA GTTCTGCTGC ACAAGTCACG 
CCAAGCAGTT GTTTAGATGG CACTGTAGTT ATTGACAAGA CTGTGGGGAA TGACACTTTC 
TTCTTGGTCA CTTGGGACTC TACAATTCCC AGCATATCAC TGATGGATCC GAATGGAAAA 
GTTTATACGG AGGCTGATTT CAAAAGTGAT CAAGTTTCAA AATCTGCTCG TCTCCTGATT 
CCCGGCACAG CACAGAATGG GAAATGGCAG TACAGACTGT GTAGCACTAT ACTTATTGAT 
CAAGTAATCG GCATAACAGT CAATACCCGT GCAGCGAATG AAGATGTGCC ACCCATAGTT 
GCAGAGGCTT ACATGAGTGA TGATACAGGC ATGTACCCTC AGCCAATGTT GGTGTATGCC 
ATTGTTACCC AGGGTCTTGC ACCTGTTTTG GGAGCAACAG TTACTGCTAT TATTGAACCA 
CAGAATGGAA ACATTCAGAC TTTGCAGCTT TTGGATAATG GAGCAGGTGC GGATATTATC 
AAGAATGACG GTATCTATTC CAAATATTTT ATTCAGTTCA CTGGAAATGG TAGATACAAC 
TTGAAAGTGC GTGTAGAAAA CAAAGAGAAA AGCGCCAACC TAGCAGCGCC AGAAAGTCGT 
GCCCTTTACC TGCCTGGCTT CATTGTGAAT GGTACTGTGT TCCGTAACCC ACCAAGGCCA 
AAAGATAAGG TTATTGAAGA CCAAAGTTTG GGAGACTTTA CCAGGACAGT TTCTGGTGGT 
GCCTTCACTG TAACAAATGT GCCATCAGGG CCTCTACCAG ACACATTTAA ACCTGATAGA 
ATTACTGATT TGGAAGCAAC AATTAAAGGA AAAGAAGTCG AAGTAACTTG GACAGCCACT 
GGTGATGACC TGGACCAGGG AAATGCTTCG ATATATGATT TAAGAATGAG CCTCAGTTCT 
AAGGATCTTT CAGAAAACTT TGAGAATGCC ACCACCATAA ATGTCACCCA CCTCACTCCA 
CAGCAGGCTG GCAGCAGGGA AACCTTTATA TTTACACCAG AAAATGTGAC CATACAAAAC 
GGCACCATCA TTTTTTTTGC ACTGATTGCC TTTGATGATG TTGGACTGAA ATCCGACCCC 
TCTAACTTAG CAAAGGCAAC TTTCTTTGTA CCACAGACAA ACCCAGGCAA TGGCTCAAAT 
CACAGCAATT TTAATAAACT GACCATCATA TTGGTGGGAC TGATGGTCAT GGTAACCCTG 
GTGATTAATT TGGTGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817908.1
CDS	293..3013
Translation	MRLLHILALVSAFQSVHLARDSLVQLKNNGYEDIIIAVNPQVPEDGKIIENIKKMLTDASSYLFQATKKRLYIRSAKILIPNTWATNSSYGRPKLESYDKADVIVAPPFVQGDDPYTLQFGGCGEKGKYIHFTPNFLVNDEKMLPIYGPRGRVFVHEWAHFRWGVFDEYNYNRPYYFSENRKVEATRCPLKLKGLNLIDVCQRGVCNLEPCEYDKNTGLYEEDCKFYPDRDILVEESVMYAQMFEPVHAFCDSSSHNSEAPNQQNRLCSQQSTWDVISKSSDIKSSPPLKDSNIPAPVISLLQYKDRVVSLVLDISGSMTNANRITRLYQASEVYIMQIVEQGAYVGIVTFSNVAEIKSQLVKITDTFQRESLKLKLPTVATGGTNICAGVQQGLQVNRNLDQSTHGTEIVLLTDGEDSGISSCFPDITKSGAIIHTIALGNNADPGLEKLADLTGGLKLYASDKVDANGLIDSFSGIVSNTGNVTQQSLQIESSAAQVTPSSCLDGTVVIDKTVGNDTFFLVTWDSTIPSISLMDPNGKVYTEADFKSDQVSKSARLLIPGTAQNGKWQYRLCSTILIDQVIGITVNTRAANEDVPPIVAEAYMSDDTGMYPQPMLVYAIVTQGLAPVLGATVTAIIEPQNGNIQTLQLLDNGAGADIIKNDGIYSKYFIQFTGNGRYNLKVRVENKEKSANLAAPESRALYLPGFIVNGTVFRNPPRPKDKVIEDQSLGDFTRTVSGGAFTVTNVPSGPLPDTFKPDRITDLEATIKGKEVEVTWTATGDDLDQGNASIYDLRMSLSSKDLSENFENATTINVTHLTPQQAGSRETFIFTPENVTIQNGTIIFFALIAFDDVGLKSDPSNLAKATFFVPQTNPGNGSNHSNFNKLTIILVGLMVMVTLVINLVV

Target ORF information:

RefSeq Version	XM_012962454.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis epithelial chloride channel protein (LOC100158554), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962454.2

ORF Insert Sequence:

ATGAGGCTCC TGCACATTCT AGCACTGGTT TCTGCTTTCC AGTCTGTACA TTTAGCAAGA 
GACTCATTGG TGCAGCTGAA AAACAATGGA TATGAAGATA TCATTATAGC AGTTAATCCC 
CAAGTGCCTG AAGATGGGAA GATTATTGAA AATATCAAGA AAATGCTGAC CGATGCATCT 
TCTTACTTGT TCCAAGCAAC TAAAAAAAGA CTTTATATAA GGAGTGCAAA AATTTTGATC 
CCCAATACTT GGGCAACCAA TAGTTCATAT GGTAGGCCGA AACTAGAGTC ATATGACAAG 
GCAGATGTGA TTGTTGCACC TCCTTTTGTG CAAGGGGATG ATCCATATAC CTTACAGTTT 
GGAGGTTGTG GAGAAAAGGG AAAATACATT CATTTCACTC CTAACTTCCT TGTAAATGAT 
GAGAAAATGC TACCAATATA TGGACCAAGA GGGAGAGTTT TTGTGCATGA GTGGGCTCAC 
TTTCGCTGGG GCGTTTTTGA CGAATACAAC TATAATCGCC CTTACTATTT TTCTGAAAAC 
CGGAAAGTTG AAGCAACAAG GTGCCCTTTG AAGTTGAAAG GCCTAAATCT AATAGATGTA 
TGCCAAAGGG GAGTTTGCAA TCTGGAACCT TGTGAATATG ACAAGAACAC AGGGCTCTAT 
GAAGAGGACT GCAAATTCTA TCCTGACAGA GATATCTTGG TGGAGGAATC TGTTATGTAT 
GCCCAAATGT TTGAACCTGT ACATGCCTTC TGTGATAGCA GTAGCCATAA TTCAGAGGCT 
CCCAACCAAC AAAACCGCCT GTGCAGCCAA CAGAGCACAT GGGATGTCAT CAGTAAGTCT 
TCCGACATAA AATCCAGTCC TCCTTTAAAG GACTCAAACA TTCCAGCTCC TGTGATTTCT 
CTATTGCAAT ACAAGGACAG AGTGGTCTCA TTGGTTCTGG ATATATCTGG AAGCATGACA 
AATGCTAACC GCATTACCCG GCTGTATCAA GCATCAGAAG TGTACATCAT GCAAATAGTG 
GAACAGGGTG CTTATGTTGG TATCGTTACT TTTAGCAATG TGGCCGAGAT CAAGTCACAA 
CTGGTTAAAA TAACGGATAC TTTTCAGCGG GAAAGTCTGA AGTTAAAGCT ACCAACTGTA 
GCGACTGGTG GTACAAACAT CTGTGCGGGA GTTCAACAAG GTTTGCAGGT AAATAGAAAT 
CTTGATCAGA GCACACATGG CACTGAAATT GTGCTGTTGA CAGATGGAGA AGATTCTGGA 
ATTAGCAGTT GTTTTCCTGA CATTACTAAG AGCGGAGCCA TTATCCACAC AATTGCACTT 
GGAAACAATG CAGACCCCGG GCTCGAAAAG CTTGCAGATC TCACAGGTGG CCTAAAACTG 
TATGCATCAG ATAAAGTAGA TGCAAATGGC CTGATTGATT CTTTCAGTGG GATTGTGTCC 
AACACTGGGA ATGTAACACA GCAATCCCTT CAGATTGAAA GTTCTGCTGC ACAAGTCACG 
CCAAGCAGTT GTTTAGATGG CACTGTAGTT ATTGACAAGA CTGTGGGGAA TGACACTTTC 
TTCTTGGTCA CTTGGGACTC TACAATTCCC AGCATATCAC TGATGGATCC GAATGGAAAA 
GTTTATACGG AGGCTGATTT CAAAAGTGAT CAAGTTTCAA AATCTGCTCG TCTCCTGATT 
CCCGGCACAG CACAGAATGG GAAATGGCAG TACAGACTGT GTAGCACTAT ACTTATTGAT 
CAAGTAATCG GCATAACAGT CAATACCCGT GCAGCGAATG AAGATGTGCC ACCCATAGTT 
GCAGAGGCTT ACATGAGTGA TGATACAGGC ATGTACCCTC AGCCAATGTT GGTGTATGCC 
ATTGTTACCC AGGGTCTTGC ACCTGTTTTG GGAGCAACAG TTACTGCTAT TATTGAACCA 
CAGAATGGAA ACATTCAGAC TTTGCAGCTT TTGGATAATG GAGCAGGTGC GGATATTATC 
AAGAATGACG GTATCTATTC CAAATATTTT ATTCAGTTCA CTGGAAATGG TAGATACAAC 
TTGAAAGTGC GTGTAGAAAA CAAAGAGAAA AGCGCCAACC TAGCAGCGCC AGAAAGTCGT 
GCCCTTTACC TGCCTGGCTT CATTGTGAAT GGTACTGTGT TCCGTAACCC ACCAAGGCCA 
AAAGATAAGG TTATTGAAGA CCAAAGTTTG GGAGACTTTA CCAGGACAGT TTCTGGTGGT 
GCCTTCACTG TAACAAATGT GCCATCAGGG CCTCTACCAG ACACATTTAA ACCTGATAGA 
ATTACTGATT TGGAAGCAAC AATTAAAGGA AAAGAAGTCG AAGTAACTTG GACAGCCACT 
GGTGATGACC TGGACCAGGG AAATGCTTCG ATATATGATT TAAGAATGAG CCTCAGTTCT 
AAGGATCTTT CAGAAAACTT TGAGAATGCC ACCACCATAA ATGTCACCCA CCTCACTCCA 
CAGCAGGCTG GCAGCAGGGA AACCTTTATA TTTACACCAG AAAATGTGAC CATACAAAAC 
GGCACCATCA TTTTTTTTGC ACTGATTGCC TTTGATGATG TTGGACTGAA ATCCGACCCC 
TCTAACTTAG CAAAGGCAAC TTTCTTTGTA CCACAGACAA ACCCAGGCAA TGGCTCAAAT 
CACAGCAATT TTAATAAACT GACCATCATA TTGGTGGGAC TGATGGTCAT GGTAACCCTG 
GTGATTAATT TGGTGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100158554 ( XM_002934158.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934204.1 Xenopus tropicalis epithelial chloride channel protein (LOC100158554), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa09078
Clone ID Related Accession (Same CDS sequence)	XM_012962454.2 , XM_002934158.4
Accession Version	XM_002934158.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	epithelial chloride channel protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934158.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTCC TGCACATTCT AGCACTGGTT TCTGCTTTCC AGTCTGTACA TTTAGCAAGA 
GACTCATTGG TGCAGCTGAA AAACAATGGA TATGAAGATA TCATTATAGC AGTTAATCCC 
CAAGTGCCTG AAGATGGGAA GATTATTGAA AATATCAAGA AAATGCTGAC CGATGCATCT 
TCTTACTTGT TCCAAGCAAC TAAAAAAAGA CTTTATATAA GGAGTGCAAA AATTTTGATC 
CCCAATACTT GGGCAACCAA TAGTTCATAT GGTAGGCCGA AACTAGAGTC ATATGACAAG 
GCAGATGTGA TTGTTGCACC TCCTTTTGTG CAAGGGGATG ATCCATATAC CTTACAGTTT 
GGAGGTTGTG GAGAAAAGGG AAAATACATT CATTTCACTC CTAACTTCCT TGTAAATGAT 
GAGAAAATGC TACCAATATA TGGACCAAGA GGGAGAGTTT TTGTGCATGA GTGGGCTCAC 
TTTCGCTGGG GCGTTTTTGA CGAATACAAC TATAATCGCC CTTACTATTT TTCTGAAAAC 
CGGAAAGTTG AAGCAACAAG GTGCCCTTTG AAGTTGAAAG GCCTAAATCT AATAGATGTA 
TGCCAAAGGG GAGTTTGCAA TCTGGAACCT TGTGAATATG ACAAGAACAC AGGGCTCTAT 
GAAGAGGACT GCAAATTCTA TCCTGACAGA GATATCTTGG TGGAGGAATC TGTTATGTAT 
GCCCAAATGT TTGAACCTGT ACATGCCTTC TGTGATAGCA GTAGCCATAA TTCAGAGGCT 
CCCAACCAAC AAAACCGCCT GTGCAGCCAA CAGAGCACAT GGGATGTCAT CAGTAAGTCT 
TCCGACATAA AATCCAGTCC TCCTTTAAAG GACTCAAACA TTCCAGCTCC TGTGATTTCT 
CTATTGCAAT ACAAGGACAG AGTGGTCTCA TTGGTTCTGG ATATATCTGG AAGCATGACA 
AATGCTAACC GCATTACCCG GCTGTATCAA GCATCAGAAG TGTACATCAT GCAAATAGTG 
GAACAGGGTG CTTATGTTGG TATCGTTACT TTTAGCAATG TGGCCGAGAT CAAGTCACAA 
CTGGTTAAAA TAACGGATAC TTTTCAGCGG GAAAGTCTGA AGTTAAAGCT ACCAACTGTA 
GCGACTGGTG GTACAAACAT CTGTGCGGGA GTTCAACAAG GTTTGCAGGT AAATAGAAAT 
CTTGATCAGA GCACACATGG CACTGAAATT GTGCTGTTGA CAGATGGAGA AGATTCTGGA 
ATTAGCAGTT GTTTTCCTGA CATTACTAAG AGCGGAGCCA TTATCCACAC AATTGCACTT 
GGAAACAATG CAGACCCCGG GCTCGAAAAG CTTGCAGATC TCACAGGTGG CCTAAAACTG 
TATGCATCAG ATAAAGTAGA TGCAAATGGC CTGATTGATT CTTTCAGTGG GATTGTGTCC 
AACACTGGGA ATGTAACACA GCAATCCCTT CAGATTGAAA GTTCTGCTGC ACAAGTCACG 
CCAAGCAGTT GTTTAGATGG CACTGTAGTT ATTGACAAGA CTGTGGGGAA TGACACTTTC 
TTCTTGGTCA CTTGGGACTC TACAATTCCC AGCATATCAC TGATGGATCC GAATGGAAAA 
GTTTATACGG AGGCTGATTT CAAAAGTGAT CAAGTTTCAA AATCTGCTCG TCTCCTGATT 
CCCGGCACAG CACAGAATGG GAAATGGCAG TACAGACTGT GTAGCACTAT ACTTATTGAT 
CAAGTAATCG GCATAACAGT CAATACCCGT GCAGCGAATG AAGATGTGCC ACCCATAGTT 
GCAGAGGCTT ACATGAGTGA TGATACAGGC ATGTACCCTC AGCCAATGTT GGTGTATGCC 
ATTGTTACCC AGGGTCTTGC ACCTGTTTTG GGAGCAACAG TTACTGCTAT TATTGAACCA 
CAGAATGGAA ACATTCAGAC TTTGCAGCTT TTGGATAATG GAGCAGGTGC GGATATTATC 
AAGAATGACG GTATCTATTC CAAATATTTT ATTCAGTTCA CTGGAAATGG TAGATACAAC 
TTGAAAGTGC GTGTAGAAAA CAAAGAGAAA AGCGCCAACC TAGCAGCGCC AGAAAGTCGT 
GCCCTTTACC TGCCTGGCTT CATTGTGAAT GGTACTGTGT TCCGTAACCC ACCAAGGCCA 
AAAGATAAGG TTATTGAAGA CCAAAGTTTG GGAGACTTTA CCAGGACAGT TTCTGGTGGT 
GCCTTCACTG TAACAAATGT GCCATCAGGG CCTCTACCAG ACACATTTAA ACCTGATAGA 
ATTACTGATT TGGAAGCAAC AATTAAAGGA AAAGAAGTCG AAGTAACTTG GACAGCCACT 
GGTGATGACC TGGACCAGGG AAATGCTTCG ATATATGATT TAAGAATGAG CCTCAGTTCT 
AAGGATCTTT CAGAAAACTT TGAGAATGCC ACCACCATAA ATGTCACCCA CCTCACTCCA 
CAGCAGGCTG GCAGCAGGGA AACCTTTATA TTTACACCAG AAAATGTGAC CATACAAAAC 
GGCACCATCA TTTTTTTTGC ACTGATTGCC TTTGATGATG TTGGACTGAA ATCCGACCCC 
TCTAACTTAG CAAAGGCAAC TTTCTTTGTA CCACAGACAA ACCCAGGCAA TGGCTCAAAT 
CACAGCAATT TTAATAAACT GACCATCATA TTGGTGGGAC TGATGGTCAT GGTAACCCTG 
GTGATTAATT TGGTGGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934204.1
CDS	285..3005
Translation	MRLLHILALVSAFQSVHLARDSLVQLKNNGYEDIIIAVNPQVPEDGKIIENIKKMLTDASSYLFQATKKRLYIRSAKILIPNTWATNSSYGRPKLESYDKADVIVAPPFVQGDDPYTLQFGGCGEKGKYIHFTPNFLVNDEKMLPIYGPRGRVFVHEWAHFRWGVFDEYNYNRPYYFSENRKVEATRCPLKLKGLNLIDVCQRGVCNLEPCEYDKNTGLYEEDCKFYPDRDILVEESVMYAQMFEPVHAFCDSSSHNSEAPNQQNRLCSQQSTWDVISKSSDIKSSPPLKDSNIPAPVISLLQYKDRVVSLVLDISGSMTNANRITRLYQASEVYIMQIVEQGAYVGIVTFSNVAEIKSQLVKITDTFQRESLKLKLPTVATGGTNICAGVQQGLQVNRNLDQSTHGTEIVLLTDGEDSGISSCFPDITKSGAIIHTIALGNNADPGLEKLADLTGGLKLYASDKVDANGLIDSFSGIVSNTGNVTQQSLQIESSAAQVTPSSCLDGTVVIDKTVGNDTFFLVTWDSTIPSISLMDPNGKVYTEADFKSDQVSKSARLLIPGTAQNGKWQYRLCSTILIDQVIGITVNTRAANEDVPPIVAEAYMSDDTGMYPQPMLVYAIVTQGLAPVLGATVTAIIEPQNGNIQTLQLLDNGAGADIIKNDGIYSKYFIQFTGNGRYNLKVRVENKEKSANLAAPESRALYLPGFIVNGTVFRNPPRPKDKVIEDQSLGDFTRTVSGGAFTVTNVPSGPLPDTFKPDRITDLEATIKGKEVEVTWTATGDDLDQGNASIYDLRMSLSSKDLSENFENATTINVTHLTPQQAGSRETFIFTPENVTIQNGTIIFFALIAFDDVGLKSDPSNLAKATFFVPQTNPGNGSNHSNFNKLTIILVGLMVMVTLVINLVV

Target ORF information:

RefSeq Version	XM_002934158.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis epithelial chloride channel protein (LOC100158554), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934158.4

ORF Insert Sequence:

ATGAGGCTCC TGCACATTCT AGCACTGGTT TCTGCTTTCC AGTCTGTACA TTTAGCAAGA 
GACTCATTGG TGCAGCTGAA AAACAATGGA TATGAAGATA TCATTATAGC AGTTAATCCC 
CAAGTGCCTG AAGATGGGAA GATTATTGAA AATATCAAGA AAATGCTGAC CGATGCATCT 
TCTTACTTGT TCCAAGCAAC TAAAAAAAGA CTTTATATAA GGAGTGCAAA AATTTTGATC 
CCCAATACTT GGGCAACCAA TAGTTCATAT GGTAGGCCGA AACTAGAGTC ATATGACAAG 
GCAGATGTGA TTGTTGCACC TCCTTTTGTG CAAGGGGATG ATCCATATAC CTTACAGTTT 
GGAGGTTGTG GAGAAAAGGG AAAATACATT CATTTCACTC CTAACTTCCT TGTAAATGAT 
GAGAAAATGC TACCAATATA TGGACCAAGA GGGAGAGTTT TTGTGCATGA GTGGGCTCAC 
TTTCGCTGGG GCGTTTTTGA CGAATACAAC TATAATCGCC CTTACTATTT TTCTGAAAAC 
CGGAAAGTTG AAGCAACAAG GTGCCCTTTG AAGTTGAAAG GCCTAAATCT AATAGATGTA 
TGCCAAAGGG GAGTTTGCAA TCTGGAACCT TGTGAATATG ACAAGAACAC AGGGCTCTAT 
GAAGAGGACT GCAAATTCTA TCCTGACAGA GATATCTTGG TGGAGGAATC TGTTATGTAT 
GCCCAAATGT TTGAACCTGT ACATGCCTTC TGTGATAGCA GTAGCCATAA TTCAGAGGCT 
CCCAACCAAC AAAACCGCCT GTGCAGCCAA CAGAGCACAT GGGATGTCAT CAGTAAGTCT 
TCCGACATAA AATCCAGTCC TCCTTTAAAG GACTCAAACA TTCCAGCTCC TGTGATTTCT 
CTATTGCAAT ACAAGGACAG AGTGGTCTCA TTGGTTCTGG ATATATCTGG AAGCATGACA 
AATGCTAACC GCATTACCCG GCTGTATCAA GCATCAGAAG TGTACATCAT GCAAATAGTG 
GAACAGGGTG CTTATGTTGG TATCGTTACT TTTAGCAATG TGGCCGAGAT CAAGTCACAA 
CTGGTTAAAA TAACGGATAC TTTTCAGCGG GAAAGTCTGA AGTTAAAGCT ACCAACTGTA 
GCGACTGGTG GTACAAACAT CTGTGCGGGA GTTCAACAAG GTTTGCAGGT AAATAGAAAT 
CTTGATCAGA GCACACATGG CACTGAAATT GTGCTGTTGA CAGATGGAGA AGATTCTGGA 
ATTAGCAGTT GTTTTCCTGA CATTACTAAG AGCGGAGCCA TTATCCACAC AATTGCACTT 
GGAAACAATG CAGACCCCGG GCTCGAAAAG CTTGCAGATC TCACAGGTGG CCTAAAACTG 
TATGCATCAG ATAAAGTAGA TGCAAATGGC CTGATTGATT CTTTCAGTGG GATTGTGTCC 
AACACTGGGA ATGTAACACA GCAATCCCTT CAGATTGAAA GTTCTGCTGC ACAAGTCACG 
CCAAGCAGTT GTTTAGATGG CACTGTAGTT ATTGACAAGA CTGTGGGGAA TGACACTTTC 
TTCTTGGTCA CTTGGGACTC TACAATTCCC AGCATATCAC TGATGGATCC GAATGGAAAA 
GTTTATACGG AGGCTGATTT CAAAAGTGAT CAAGTTTCAA AATCTGCTCG TCTCCTGATT 
CCCGGCACAG CACAGAATGG GAAATGGCAG TACAGACTGT GTAGCACTAT ACTTATTGAT 
CAAGTAATCG GCATAACAGT CAATACCCGT GCAGCGAATG AAGATGTGCC ACCCATAGTT 
GCAGAGGCTT ACATGAGTGA TGATACAGGC ATGTACCCTC AGCCAATGTT GGTGTATGCC 
ATTGTTACCC AGGGTCTTGC ACCTGTTTTG GGAGCAACAG TTACTGCTAT TATTGAACCA 
CAGAATGGAA ACATTCAGAC TTTGCAGCTT TTGGATAATG GAGCAGGTGC GGATATTATC 
AAGAATGACG GTATCTATTC CAAATATTTT ATTCAGTTCA CTGGAAATGG TAGATACAAC 
TTGAAAGTGC GTGTAGAAAA CAAAGAGAAA AGCGCCAACC TAGCAGCGCC AGAAAGTCGT 
GCCCTTTACC TGCCTGGCTT CATTGTGAAT GGTACTGTGT TCCGTAACCC ACCAAGGCCA 
AAAGATAAGG TTATTGAAGA CCAAAGTTTG GGAGACTTTA CCAGGACAGT TTCTGGTGGT 
GCCTTCACTG TAACAAATGT GCCATCAGGG CCTCTACCAG ACACATTTAA ACCTGATAGA 
ATTACTGATT TGGAAGCAAC AATTAAAGGA AAAGAAGTCG AAGTAACTTG GACAGCCACT 
GGTGATGACC TGGACCAGGG AAATGCTTCG ATATATGATT TAAGAATGAG CCTCAGTTCT 
AAGGATCTTT CAGAAAACTT TGAGAATGCC ACCACCATAA ATGTCACCCA CCTCACTCCA 
CAGCAGGCTG GCAGCAGGGA AACCTTTATA TTTACACCAG AAAATGTGAC CATACAAAAC 
GGCACCATCA TTTTTTTTGC ACTGATTGCC TTTGATGATG TTGGACTGAA ATCCGACCCC 
TCTAACTTAG CAAAGGCAAC TTTCTTTGTA CCACAGACAA ACCCAGGCAA TGGCTCAAAT 
CACAGCAATT TTAATAAACT GACCATCATA TTGGTGGGAC TGATGGTCAT GGTAACCCTG 
GTGATTAATT TGGTGGTTTA A