cgnl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cgnl1
Entrez Gene ID	100145705
Full Name	cingulin-like 1
General protein information	Preferred Names cingulin-like 1 Names cingulin-like protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018092419.2	XP_017947908.2	cingulin-like protein 1 isoform X2
XM_018092418.1	XP_017947907.1	cingulin-like protein 1 isoform X1
XM_018092419.1	XP_017947908.1	cingulin-like protein 1 isoform X2
XM_018092417.2	XP_017947906.2	cingulin-like protein 1 isoform X1
XM_018092418.2	XP_017947907.2	cingulin-like protein 1 isoform X1
XM_018092417.1	XP_017947906.1	cingulin-like protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CGNL1	NP_116255.2
Danio rerio (zebrafish)	cgnl1	XP_003199047.1
Gallus gallus (chicken)	CGNL1	XP_413790.4
	CGNL1	XP_001092704.1
Mus musculus (house mouse)	Cgnl1	NP_080875.3
Pan troglodytes (chimpanzee)	CGNL1	XP_510437.2
Bos taurus (cattle)	CGNL1	XP_005211807.1
Xenopus tropicalis (tropical clawed frog)	cgnl1	NP_001120551.1
Canis lupus familiaris (dog)	CGNL1	XP_005638512.1
Rattus norvegicus (Norway rat)	Cgnl1	NP_001101634.1

Gene Ontology

The following cgnl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cgnl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa43356	XM_018092417.1 Latest version!	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa43356	XM_018092418.1 Latest version!	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa43357	XM_018092419.1 Latest version!	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa55067	XM_018092417.2 Latest version!	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa55067	XM_018092418.2 Latest version!	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa55068	XM_018092419.2 Latest version!	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cgnl1 ( XM_018092418.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947907.1 Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa43356
Clone ID Related Accession (Same CDS sequence)	XM_018092418.1 , XM_018092417.1
Accession Version	XM_018092418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cingulin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAGGA GCAAAGTTTA TGGATCCTTG CAGAATCAGA AGGAAAAAAA CACTAATGCA 
GATGAACCAG ACAGCATATC CTACCTTTCA TCCACTTTGC CAAGCAGAGG TAAAAAGTAT 
GATGATGTAA AAATGATTAA ATCGACTCTA CAGTTAAAGG ACTGCATTTC TAGCCCACCA 
CGGGAGGGGA TAAGGAGAGC TGCAGCTACG ATGCAGGGCT CATCTCAAGT TGCTCAGGCG 
ACGCCTGACC TTTTGAAGGG ACAACAACAA GTTGCACTAC AAACAAATGA GGAAACTGCA 
AAACAAATAC TATTTAACTA TCTAAAAGAA GGGAGCCCTG ATAATGATGA TGCAACCAAA 
AGAAAGGTCA ACCTGGTGTT TGAAAAAATC CAGACTTTAA AATCAAGAGC TGCTAACACA 
GTACAGGATC CATCTGCAGA TATCAAAGTT TTACTGGAAC AGAAAAAAGA ACTGGAAAAG 
AGAGTTGCTG CTTTGCAAGA AAGACTTGAT GAAGAAATTA AGAATCACAA ACGTGACAAA 
GAAGACTCAA ATACCAAAGC AAACATGAAA GACTTCCATG ACCAGCTGGA GCAAAGCATG 
GATGAAAATC TTGTTCTTCG GAAGAGATTG GGTGAAAGTG AAAAGGAATT AAGAAAGCAT 
CTTGATGAAT TGTTTGAACT GAAGATGGAA CAAGAGCAGT ATCAGACGGA GATCAGAGAC 
CTGCAGGAAC AGTTGTCAGA AATGCACGAT GAATTGGATA ATGCCAAGAG CTCTGTTGAT 
GATGGGGAAA GGGAAGCATT TATAGAGGAG CTGATGCAAA TGAAACAAAG TTTTCAAGAG 
GTCTTGTTGG CTAAAGAAGA GCAGGAAGAC TTATTAAGGA AACGGGAGCG TGAACTTACT 
GCACTGAAAG GAGCACTGAA GGAAGAGGTA GCAACTCATG ACCAGGAACT TGATAAGCTC 
AAGGAGCAGT ATGATAAGGA GCTGAAAAGC ATCAGAGACA ATCTGACTGC AGCAGTAAAG 
AATTCCAGTG ATATAGCTAA TCAGAAGGAT GAGGCAAATC AACTGAAAGC TGTCTTTGAA 
AACAGAGTGA AAGGCCTTTT GCAAGAAAAC GAGGAGCTAA AAAGTAAAAT AGAGAACCTT 
GAGGCCAAAA TTGCTGCACT TCGCCAGGAA ATTAAAGACT TAAATGAAGA GAATGAGAGA 
ATTAAAGACA AACTTTCAAA GTCTGAGAAG GAAAAGCAGC AGTTGGCAAT TGCCTTGGAA 
AATTCTAAGG ACCAAGAAAA GGAAGCTCTG CAGTTTGCAA GAAAGCTCGA AAATCAACTT 
GAAGACACTA AGAGAAGTCT CAGCCGAGTG TCTCATGAGC ACCAGCAATT AACAGAGAGA 
TTAAAGGAAG AAGCTAACCA GAAAGAACAG CTAAGAAATC TGAAAAATGA AATGGAAAAT 
GAGCGTTGGC AGTTGGACAA GACAATTGAA AAATTACAAA AAGAGATTTC AGAAAATGTT 
GAGTTTTCAC GGATGTCCAC AGTGGAACTT CAGAAGCAGT TTGATGAATA TAAAGACAAG 
TATCGCAGGG AAAGTGCTGA AATGCAGCGG CAGATAAAAG ACAAAAATCT GGAAGTAGAA 
AGGGCTTGCC AGACTATCAA AAAACTGCAA GATGAGGTCC GCCATTTGGA GGAGAATTTG 
CAAGATCACC AGAGAGCCCA GGATGAGGCT CTAACTAAGA ATCATCTTCT TCAGCAGACC 
ATTAAAGACC TTCAGTATGA ACTGGATGCT AAAACTCATG TGAAGGACGA TCGGATTAGG 
CAGATCAAAT CAATGGAGGA CAAGTTATCG CAGCTAGAAA TGGAACTGGA TGAAGAGAAG 
AACAATTCAG ACCTTCTGCT AGAGAGAGTA ACCAGATGCA GAGAGCAGAT TGAGCAAACG 
AGAAGTGAAC TTTTTCAAGA AAGAGCAGCA AAACAAGATC TAGAGTGCGA TAAAATATCA 
CTGGAAAGAC AGAACAAAGA CCTGAAGAGC AGAATAGTTC ATCTTGAAAA TTCACACAGA 
TCAAATAAAG AAGGGCTTGT CGCACAGATG GAAACGAGAA TAGCAGAGCT GGAAGAACGC 
CTGGAAAATG AAGAAAGGGA ACGGGCAAGT CTCCAGCTCA ACAACCGCAG GCTGGAGCGG 
AAAGTTAAGG AGTTAATGAT GCAAGTAGAT GACGAGCACC TTACTCTAAC GGATCAGAAA 
GATCAGTTAA GTTTAAGACT GAAATCAATG AAGAGGCAAG TTGAAGAAGC TGAAGAGGAA 
ATAGACAGAC TGGAGAGCGC TAAAAAGAAA CTGCAGAGGG ATCTAGAAGA GCAAACTGAT 
ATAAACGACC AACTTCAAAC TCAACTTACT GCTGCTAAAA AGGAACTTAG CAGACGTAAG 
AGTTCTCCTA GCCGAATGAA TGATCTTGAG GATGATGATG ACGACCTGAG CACTGACGGG 
GAGAGTCTTT ATGATGGTCC ATCAGTATAT ACGTTTTCTA GGGATGATGG TATAAGGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947907.1
CDS	1338..3860
Translation	MPRSKVYGSLQNQKEKNTNADEPDSISYLSSTLPSRGKKYDDVKMIKSTLQLKDCISSPPREGIRRAAATMQGSSQVAQATPDLLKGQQQVALQTNEETAKQILFNYLKEGSPDNDDATKRKVNLVFEKIQTLKSRAANTVQDPSADIKVLLEQKKELEKRVAALQERLDEEIKNHKRDKEDSNTKANMKDFHDQLEQSMDENLVLRKRLGESEKELRKHLDELFELKMEQEQYQTEIRDLQEQLSEMHDELDNAKSSVDDGEREAFIEELMQMKQSFQEVLLAKEEQEDLLRKRERELTALKGALKEEVATHDQELDKLKEQYDKELKSIRDNLTAAVKNSSDIANQKDEANQLKAVFENRVKGLLQENEELKSKIENLEAKIAALRQEIKDLNEENERIKDKLSKSEKEKQQLAIALENSKDQEKEALQFARKLENQLEDTKRSLSRVSHEHQQLTERLKEEANQKEQLRNLKNEMENERWQLDKTIEKLQKEISENVEFSRMSTVELQKQFDEYKDKYRRESAEMQRQIKDKNLEVERACQTIKKLQDEVRHLEENLQDHQRAQDEALTKNHLLQQTIKDLQYELDAKTHVKDDRIRQIKSMEDKLSQLEMELDEEKNNSDLLLERVTRCREQIEQTRSELFQERAAKQDLECDKISLERQNKDLKSRIVHLENSHRSNKEGLVAQMETRIAELEERLENEERERASLQLNNRRLERKVKELMMQVDDEHLTLTDQKDQLSLRLKSMKRQVEEAEEEIDRLESAKKKLQRDLEEQTDINDQLQTQLTAAKKELSRRKSSPSRMNDLEDDDDDLSTDGESLYDGPSVYTFSRDDGIRA

Target ORF information:

RefSeq Version	XM_018092418.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092418.1

ORF Insert Sequence:

ATGCCCAGGA GCAAAGTTTA TGGATCCTTG CAGAATCAGA AGGAAAAAAA CACTAATGCA 
GATGAACCAG ACAGCATATC CTACCTTTCA TCCACTTTGC CAAGCAGAGG TAAAAAGTAT 
GATGATGTAA AAATGATTAA ATCGACTCTA CAGTTAAAGG ACTGCATTTC TAGCCCACCA 
CGGGAGGGGA TAAGGAGAGC TGCAGCTACG ATGCAGGGCT CATCTCAAGT TGCTCAGGCG 
ACGCCTGACC TTTTGAAGGG ACAACAACAA GTTGCACTAC AAACAAATGA GGAAACTGCA 
AAACAAATAC TATTTAACTA TCTAAAAGAA GGGAGCCCTG ATAATGATGA TGCAACCAAA 
AGAAAGGTCA ACCTGGTGTT TGAAAAAATC CAGACTTTAA AATCAAGAGC TGCTAACACA 
GTACAGGATC CATCTGCAGA TATCAAAGTT TTACTGGAAC AGAAAAAAGA ACTGGAAAAG 
AGAGTTGCTG CTTTGCAAGA AAGACTTGAT GAAGAAATTA AGAATCACAA ACGTGACAAA 
GAAGACTCAA ATACCAAAGC AAACATGAAA GACTTCCATG ACCAGCTGGA GCAAAGCATG 
GATGAAAATC TTGTTCTTCG GAAGAGATTG GGTGAAAGTG AAAAGGAATT AAGAAAGCAT 
CTTGATGAAT TGTTTGAACT GAAGATGGAA CAAGAGCAGT ATCAGACGGA GATCAGAGAC 
CTGCAGGAAC AGTTGTCAGA AATGCACGAT GAATTGGATA ATGCCAAGAG CTCTGTTGAT 
GATGGGGAAA GGGAAGCATT TATAGAGGAG CTGATGCAAA TGAAACAAAG TTTTCAAGAG 
GTCTTGTTGG CTAAAGAAGA GCAGGAAGAC TTATTAAGGA AACGGGAGCG TGAACTTACT 
GCACTGAAAG GAGCACTGAA GGAAGAGGTA GCAACTCATG ACCAGGAACT TGATAAGCTC 
AAGGAGCAGT ATGATAAGGA GCTGAAAAGC ATCAGAGACA ATCTGACTGC AGCAGTAAAG 
AATTCCAGTG ATATAGCTAA TCAGAAGGAT GAGGCAAATC AACTGAAAGC TGTCTTTGAA 
AACAGAGTGA AAGGCCTTTT GCAAGAAAAC GAGGAGCTAA AAAGTAAAAT AGAGAACCTT 
GAGGCCAAAA TTGCTGCACT TCGCCAGGAA ATTAAAGACT TAAATGAAGA GAATGAGAGA 
ATTAAAGACA AACTTTCAAA GTCTGAGAAG GAAAAGCAGC AGTTGGCAAT TGCCTTGGAA 
AATTCTAAGG ACCAAGAAAA GGAAGCTCTG CAGTTTGCAA GAAAGCTCGA AAATCAACTT 
GAAGACACTA AGAGAAGTCT CAGCCGAGTG TCTCATGAGC ACCAGCAATT AACAGAGAGA 
TTAAAGGAAG AAGCTAACCA GAAAGAACAG CTAAGAAATC TGAAAAATGA AATGGAAAAT 
GAGCGTTGGC AGTTGGACAA GACAATTGAA AAATTACAAA AAGAGATTTC AGAAAATGTT 
GAGTTTTCAC GGATGTCCAC AGTGGAACTT CAGAAGCAGT TTGATGAATA TAAAGACAAG 
TATCGCAGGG AAAGTGCTGA AATGCAGCGG CAGATAAAAG ACAAAAATCT GGAAGTAGAA 
AGGGCTTGCC AGACTATCAA AAAACTGCAA GATGAGGTCC GCCATTTGGA GGAGAATTTG 
CAAGATCACC AGAGAGCCCA GGATGAGGCT CTAACTAAGA ATCATCTTCT TCAGCAGACC 
ATTAAAGACC TTCAGTATGA ACTGGATGCT AAAACTCATG TGAAGGACGA TCGGATTAGG 
CAGATCAAAT CAATGGAGGA CAAGTTATCG CAGCTAGAAA TGGAACTGGA TGAAGAGAAG 
AACAATTCAG ACCTTCTGCT AGAGAGAGTA ACCAGATGCA GAGAGCAGAT TGAGCAAACG 
AGAAGTGAAC TTTTTCAAGA AAGAGCAGCA AAACAAGATC TAGAGTGCGA TAAAATATCA 
CTGGAAAGAC AGAACAAAGA CCTGAAGAGC AGAATAGTTC ATCTTGAAAA TTCACACAGA 
TCAAATAAAG AAGGGCTTGT CGCACAGATG GAAACGAGAA TAGCAGAGCT GGAAGAACGC 
CTGGAAAATG AAGAAAGGGA ACGGGCAAGT CTCCAGCTCA ACAACCGCAG GCTGGAGCGG 
AAAGTTAAGG AGTTAATGAT GCAAGTAGAT GACGAGCACC TTACTCTAAC GGATCAGAAA 
GATCAGTTAA GTTTAAGACT GAAATCAATG AAGAGGCAAG TTGAAGAAGC TGAAGAGGAA 
ATAGACAGAC TGGAGAGCGC TAAAAAGAAA CTGCAGAGGG ATCTAGAAGA GCAAACTGAT 
ATAAACGACC AACTTCAAAC TCAACTTACT GCTGCTAAAA AGGAACTTAG CAGACGTAAG 
AGTTCTCCTA GCCGAATGAA TGATCTTGAG GATGATGATG ACGACCTGAG CACTGACGGG 
GAGAGTCTTT ATGATGGTCC ATCAGTATAT ACGTTTTCTA GGGATGATGG TATAAGGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cgnl1 ( XM_018092417.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947906.1 Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa43356
Clone ID Related Accession (Same CDS sequence)	XM_018092418.1 , XM_018092417.1
Accession Version	XM_018092417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cingulin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAGGA GCAAAGTTTA TGGATCCTTG CAGAATCAGA AGGAAAAAAA CACTAATGCA 
GATGAACCAG ACAGCATATC CTACCTTTCA TCCACTTTGC CAAGCAGAGG TAAAAAGTAT 
GATGATGTAA AAATGATTAA ATCGACTCTA CAGTTAAAGG ACTGCATTTC TAGCCCACCA 
CGGGAGGGGA TAAGGAGAGC TGCAGCTACG ATGCAGGGCT CATCTCAAGT TGCTCAGGCG 
ACGCCTGACC TTTTGAAGGG ACAACAACAA GTTGCACTAC AAACAAATGA GGAAACTGCA 
AAACAAATAC TATTTAACTA TCTAAAAGAA GGGAGCCCTG ATAATGATGA TGCAACCAAA 
AGAAAGGTCA ACCTGGTGTT TGAAAAAATC CAGACTTTAA AATCAAGAGC TGCTAACACA 
GTACAGGATC CATCTGCAGA TATCAAAGTT TTACTGGAAC AGAAAAAAGA ACTGGAAAAG 
AGAGTTGCTG CTTTGCAAGA AAGACTTGAT GAAGAAATTA AGAATCACAA ACGTGACAAA 
GAAGACTCAA ATACCAAAGC AAACATGAAA GACTTCCATG ACCAGCTGGA GCAAAGCATG 
GATGAAAATC TTGTTCTTCG GAAGAGATTG GGTGAAAGTG AAAAGGAATT AAGAAAGCAT 
CTTGATGAAT TGTTTGAACT GAAGATGGAA CAAGAGCAGT ATCAGACGGA GATCAGAGAC 
CTGCAGGAAC AGTTGTCAGA AATGCACGAT GAATTGGATA ATGCCAAGAG CTCTGTTGAT 
GATGGGGAAA GGGAAGCATT TATAGAGGAG CTGATGCAAA TGAAACAAAG TTTTCAAGAG 
GTCTTGTTGG CTAAAGAAGA GCAGGAAGAC TTATTAAGGA AACGGGAGCG TGAACTTACT 
GCACTGAAAG GAGCACTGAA GGAAGAGGTA GCAACTCATG ACCAGGAACT TGATAAGCTC 
AAGGAGCAGT ATGATAAGGA GCTGAAAAGC ATCAGAGACA ATCTGACTGC AGCAGTAAAG 
AATTCCAGTG ATATAGCTAA TCAGAAGGAT GAGGCAAATC AACTGAAAGC TGTCTTTGAA 
AACAGAGTGA AAGGCCTTTT GCAAGAAAAC GAGGAGCTAA AAAGTAAAAT AGAGAACCTT 
GAGGCCAAAA TTGCTGCACT TCGCCAGGAA ATTAAAGACT TAAATGAAGA GAATGAGAGA 
ATTAAAGACA AACTTTCAAA GTCTGAGAAG GAAAAGCAGC AGTTGGCAAT TGCCTTGGAA 
AATTCTAAGG ACCAAGAAAA GGAAGCTCTG CAGTTTGCAA GAAAGCTCGA AAATCAACTT 
GAAGACACTA AGAGAAGTCT CAGCCGAGTG TCTCATGAGC ACCAGCAATT AACAGAGAGA 
TTAAAGGAAG AAGCTAACCA GAAAGAACAG CTAAGAAATC TGAAAAATGA AATGGAAAAT 
GAGCGTTGGC AGTTGGACAA GACAATTGAA AAATTACAAA AAGAGATTTC AGAAAATGTT 
GAGTTTTCAC GGATGTCCAC AGTGGAACTT CAGAAGCAGT TTGATGAATA TAAAGACAAG 
TATCGCAGGG AAAGTGCTGA AATGCAGCGG CAGATAAAAG ACAAAAATCT GGAAGTAGAA 
AGGGCTTGCC AGACTATCAA AAAACTGCAA GATGAGGTCC GCCATTTGGA GGAGAATTTG 
CAAGATCACC AGAGAGCCCA GGATGAGGCT CTAACTAAGA ATCATCTTCT TCAGCAGACC 
ATTAAAGACC TTCAGTATGA ACTGGATGCT AAAACTCATG TGAAGGACGA TCGGATTAGG 
CAGATCAAAT CAATGGAGGA CAAGTTATCG CAGCTAGAAA TGGAACTGGA TGAAGAGAAG 
AACAATTCAG ACCTTCTGCT AGAGAGAGTA ACCAGATGCA GAGAGCAGAT TGAGCAAACG 
AGAAGTGAAC TTTTTCAAGA AAGAGCAGCA AAACAAGATC TAGAGTGCGA TAAAATATCA 
CTGGAAAGAC AGAACAAAGA CCTGAAGAGC AGAATAGTTC ATCTTGAAAA TTCACACAGA 
TCAAATAAAG AAGGGCTTGT CGCACAGATG GAAACGAGAA TAGCAGAGCT GGAAGAACGC 
CTGGAAAATG AAGAAAGGGA ACGGGCAAGT CTCCAGCTCA ACAACCGCAG GCTGGAGCGG 
AAAGTTAAGG AGTTAATGAT GCAAGTAGAT GACGAGCACC TTACTCTAAC GGATCAGAAA 
GATCAGTTAA GTTTAAGACT GAAATCAATG AAGAGGCAAG TTGAAGAAGC TGAAGAGGAA 
ATAGACAGAC TGGAGAGCGC TAAAAAGAAA CTGCAGAGGG ATCTAGAAGA GCAAACTGAT 
ATAAACGACC AACTTCAAAC TCAACTTACT GCTGCTAAAA AGGAACTTAG CAGACGTAAG 
AGTTCTCCTA GCCGAATGAA TGATCTTGAG GATGATGATG ACGACCTGAG CACTGACGGG 
GAGAGTCTTT ATGATGGTCC ATCAGTATAT ACGTTTTCTA GGGATGATGG TATAAGGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947906.1
CDS	1478..4000
Translation	MPRSKVYGSLQNQKEKNTNADEPDSISYLSSTLPSRGKKYDDVKMIKSTLQLKDCISSPPREGIRRAAATMQGSSQVAQATPDLLKGQQQVALQTNEETAKQILFNYLKEGSPDNDDATKRKVNLVFEKIQTLKSRAANTVQDPSADIKVLLEQKKELEKRVAALQERLDEEIKNHKRDKEDSNTKANMKDFHDQLEQSMDENLVLRKRLGESEKELRKHLDELFELKMEQEQYQTEIRDLQEQLSEMHDELDNAKSSVDDGEREAFIEELMQMKQSFQEVLLAKEEQEDLLRKRERELTALKGALKEEVATHDQELDKLKEQYDKELKSIRDNLTAAVKNSSDIANQKDEANQLKAVFENRVKGLLQENEELKSKIENLEAKIAALRQEIKDLNEENERIKDKLSKSEKEKQQLAIALENSKDQEKEALQFARKLENQLEDTKRSLSRVSHEHQQLTERLKEEANQKEQLRNLKNEMENERWQLDKTIEKLQKEISENVEFSRMSTVELQKQFDEYKDKYRRESAEMQRQIKDKNLEVERACQTIKKLQDEVRHLEENLQDHQRAQDEALTKNHLLQQTIKDLQYELDAKTHVKDDRIRQIKSMEDKLSQLEMELDEEKNNSDLLLERVTRCREQIEQTRSELFQERAAKQDLECDKISLERQNKDLKSRIVHLENSHRSNKEGLVAQMETRIAELEERLENEERERASLQLNNRRLERKVKELMMQVDDEHLTLTDQKDQLSLRLKSMKRQVEEAEEEIDRLESAKKKLQRDLEEQTDINDQLQTQLTAAKKELSRRKSSPSRMNDLEDDDDDLSTDGESLYDGPSVYTFSRDDGIRA

Target ORF information:

RefSeq Version	XM_018092417.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092417.1

ORF Insert Sequence:

ATGCCCAGGA GCAAAGTTTA TGGATCCTTG CAGAATCAGA AGGAAAAAAA CACTAATGCA 
GATGAACCAG ACAGCATATC CTACCTTTCA TCCACTTTGC CAAGCAGAGG TAAAAAGTAT 
GATGATGTAA AAATGATTAA ATCGACTCTA CAGTTAAAGG ACTGCATTTC TAGCCCACCA 
CGGGAGGGGA TAAGGAGAGC TGCAGCTACG ATGCAGGGCT CATCTCAAGT TGCTCAGGCG 
ACGCCTGACC TTTTGAAGGG ACAACAACAA GTTGCACTAC AAACAAATGA GGAAACTGCA 
AAACAAATAC TATTTAACTA TCTAAAAGAA GGGAGCCCTG ATAATGATGA TGCAACCAAA 
AGAAAGGTCA ACCTGGTGTT TGAAAAAATC CAGACTTTAA AATCAAGAGC TGCTAACACA 
GTACAGGATC CATCTGCAGA TATCAAAGTT TTACTGGAAC AGAAAAAAGA ACTGGAAAAG 
AGAGTTGCTG CTTTGCAAGA AAGACTTGAT GAAGAAATTA AGAATCACAA ACGTGACAAA 
GAAGACTCAA ATACCAAAGC AAACATGAAA GACTTCCATG ACCAGCTGGA GCAAAGCATG 
GATGAAAATC TTGTTCTTCG GAAGAGATTG GGTGAAAGTG AAAAGGAATT AAGAAAGCAT 
CTTGATGAAT TGTTTGAACT GAAGATGGAA CAAGAGCAGT ATCAGACGGA GATCAGAGAC 
CTGCAGGAAC AGTTGTCAGA AATGCACGAT GAATTGGATA ATGCCAAGAG CTCTGTTGAT 
GATGGGGAAA GGGAAGCATT TATAGAGGAG CTGATGCAAA TGAAACAAAG TTTTCAAGAG 
GTCTTGTTGG CTAAAGAAGA GCAGGAAGAC TTATTAAGGA AACGGGAGCG TGAACTTACT 
GCACTGAAAG GAGCACTGAA GGAAGAGGTA GCAACTCATG ACCAGGAACT TGATAAGCTC 
AAGGAGCAGT ATGATAAGGA GCTGAAAAGC ATCAGAGACA ATCTGACTGC AGCAGTAAAG 
AATTCCAGTG ATATAGCTAA TCAGAAGGAT GAGGCAAATC AACTGAAAGC TGTCTTTGAA 
AACAGAGTGA AAGGCCTTTT GCAAGAAAAC GAGGAGCTAA AAAGTAAAAT AGAGAACCTT 
GAGGCCAAAA TTGCTGCACT TCGCCAGGAA ATTAAAGACT TAAATGAAGA GAATGAGAGA 
ATTAAAGACA AACTTTCAAA GTCTGAGAAG GAAAAGCAGC AGTTGGCAAT TGCCTTGGAA 
AATTCTAAGG ACCAAGAAAA GGAAGCTCTG CAGTTTGCAA GAAAGCTCGA AAATCAACTT 
GAAGACACTA AGAGAAGTCT CAGCCGAGTG TCTCATGAGC ACCAGCAATT AACAGAGAGA 
TTAAAGGAAG AAGCTAACCA GAAAGAACAG CTAAGAAATC TGAAAAATGA AATGGAAAAT 
GAGCGTTGGC AGTTGGACAA GACAATTGAA AAATTACAAA AAGAGATTTC AGAAAATGTT 
GAGTTTTCAC GGATGTCCAC AGTGGAACTT CAGAAGCAGT TTGATGAATA TAAAGACAAG 
TATCGCAGGG AAAGTGCTGA AATGCAGCGG CAGATAAAAG ACAAAAATCT GGAAGTAGAA 
AGGGCTTGCC AGACTATCAA AAAACTGCAA GATGAGGTCC GCCATTTGGA GGAGAATTTG 
CAAGATCACC AGAGAGCCCA GGATGAGGCT CTAACTAAGA ATCATCTTCT TCAGCAGACC 
ATTAAAGACC TTCAGTATGA ACTGGATGCT AAAACTCATG TGAAGGACGA TCGGATTAGG 
CAGATCAAAT CAATGGAGGA CAAGTTATCG CAGCTAGAAA TGGAACTGGA TGAAGAGAAG 
AACAATTCAG ACCTTCTGCT AGAGAGAGTA ACCAGATGCA GAGAGCAGAT TGAGCAAACG 
AGAAGTGAAC TTTTTCAAGA AAGAGCAGCA AAACAAGATC TAGAGTGCGA TAAAATATCA 
CTGGAAAGAC AGAACAAAGA CCTGAAGAGC AGAATAGTTC ATCTTGAAAA TTCACACAGA 
TCAAATAAAG AAGGGCTTGT CGCACAGATG GAAACGAGAA TAGCAGAGCT GGAAGAACGC 
CTGGAAAATG AAGAAAGGGA ACGGGCAAGT CTCCAGCTCA ACAACCGCAG GCTGGAGCGG 
AAAGTTAAGG AGTTAATGAT GCAAGTAGAT GACGAGCACC TTACTCTAAC GGATCAGAAA 
GATCAGTTAA GTTTAAGACT GAAATCAATG AAGAGGCAAG TTGAAGAAGC TGAAGAGGAA 
ATAGACAGAC TGGAGAGCGC TAAAAAGAAA CTGCAGAGGG ATCTAGAAGA GCAAACTGAT 
ATAAACGACC AACTTCAAAC TCAACTTACT GCTGCTAAAA AGGAACTTAG CAGACGTAAG 
AGTTCTCCTA GCCGAATGAA TGATCTTGAG GATGATGATG ACGACCTGAG CACTGACGGG 
GAGAGTCTTT ATGATGGTCC ATCAGTATAT ACGTTTTCTA GGGATGATGG TATAAGGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cgnl1 ( XM_018092419.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947908.1 Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X3, mRNA.

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CloneID	OXa43357
Clone ID Related Accession (Same CDS sequence)	XM_018092419.1
Accession Version	XM_018092419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cingulin-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAGGA GCAAAGTTTA TGGATCCTTG CAGAATCAGA AGGAAAAAAA CACTAATGCA 
GATGAACCAG ACAGCATATC CTACCTTTCA TCCACTTTGC CAAGCAGAGG TAAAAAGTAT 
GATGATGTAA AAATGATTAA ATCGACTCTA CAGTTAAAGG ACTGCATTTC TAGCCCACCA 
CGGGAGGGGA TAAGGAGAGC TGCAGCTACG ATGCAGGGCT CATCTCAAGT TGCTCAGGCG 
ACGCCTGACC TTTTGAAGGG ACAACAACAA GTTGCACTAC AAACAAATGA GGAAACTGCA 
AAACAAATAC TATTTAACTA TCTAAAAGAA GGGAGCCCTG ATAATGATGA TGCAACCAAA 
AGAAAGGTCA ACCTGGTGTT TGAAAAAATC CAGACTTTAA AATCAAGAGC TGCTAACACA 
GTACAGGATC CATCTGCAGA TATCAAAGTT TTACTGGAAC AGAAAAAAGA ACTGGAAAAG 
AGAGTTGCTG CTTTGCAAGA AAGACTTGAT GAAGAAATTA AGAATCACAA ACGTGACAAA 
GAAGACTCAA ATACCAAAGC AAACATGAAA GACTTCCATG ACCAGCTGGA GCAAAGCATG 
GATGAAAATC TTGTTCTTCG GAAGAGATTG GGTGAAAGTG AAAAGGAATT AAGAAAGCAT 
CTTGATGAAT TGTTTGAACT GAAGATGGAA CAAGAGCAGT ATCAGACGGA GATCAGAGAC 
CTGCAGGAAC AGTTGTCAGA AATGCACGAT GAATTGGATA ATGCCAAGAG CTCTGTTGAT 
GATGGGGAAA GGGAAGCATT TATAGAGGAG CTGATGCAAA TGAAACAAAG TTTTCAAGAG 
GTCTTGTTGG CTAAAGAAGA GCAGGAAGAC TTATTAAGGA AACGGGAGCG TGAACTTACT 
GCACTGAAAG GAGCACTGAA GGAAGAGGTA GCAACTCATG ACCAGGAACT TGATAAGCTC 
AAGGAGCAGT ATGATAAGGA GCTGAAAAGC ATCAGAGACA ATCTGACTGC AGCAGTAAAG 
AATTCCAGTG ATATAGCTAA TCAGAAGGAT GAGGCAAATC AACTGAAAGC TGTCTTTGAA 
AACAGAGTGA AAGGCCTTTT GCAAGAAAAC GAGGAGCTAA AAAGTAAAAT AGAGAACCTT 
GAGGCCAAAA TTGCTGCACT TCGCCAGGAA ATTAAAGACT TAAATGAAGA GAATGAGAGA 
ATTAAAGACA AACTTTCAAA GTCTGAGAAG GAAAAGCAGC AGTTGGCAAT TGCCTTGGAA 
AATTCTAAGG ACCAAGAAAA GGAAGCTCTG CAGTTTGCAA GAAAGCTCGA AAATCAACTT 
GAAGACACTA AGAGAAGTCT CAGCCGAGTG TCTCATGAGC ACCAGCAATT AACAGAGAGA 
TTAAAGGAAG AAGCTAACCA GAAAGAACAG CTAAGAAATC TGAAAAATGA AATGGAAAAT 
GAGCGTTGGC AGTTGGACAA GACAATTGAA AAATTACAAA AAGAGATTTC AGAAAATGTT 
GAGTTTTCAC GGATGTCCAC AGTGGAACTT CAGAAGCAGT TTGATGAATA TAAAGACAAG 
TATCGCAGGG AAAGTGCTGA AATGCAGCGG CAGATAAAAG ACAAAAATCT GGAAGTAGAA 
AGGGCTTGCC AGACTATCAA AAAACTGCAA GATGAGGTCC GCCATTTGGA GGAGAATTTG 
CAAGATCACC AGAGAGCCCA GGATGAGGCT CTAACTAAGA ATCATCTTCT TCAGCAGACC 
ATTAAAGACC TTCAGTATGA ACTGGATGCT AAAACTCATG TGAAGGACGA TCGGATTAGG 
CAGATCAAAT CAATGGAGGA CAAGTTATCG CAGCTAGAAA TGGAACTGGA TGAAGAGAAG 
AACAATTCAG ACCTTCTGCT AGAGAGAGTA ACCAGATGCA GAGAGCAGAT TGAGCAAACG 
AGAAGTGAAC TTTTTCAAGA AAGAGCAGCA AAACAAGATC TAGAGTGCGA TAAAATATCA 
CTGGAAAGAC AGAACAAAGA CCTGAAGAGC AGAATAGTTC ATCTTGAAAA TTCACACAGA 
TCAAATAAAG AAGGGCTTGT CGCACAGATG GAAACGAGAA TAGCAGAGCT GGAAGAACGC 
CTGGAAAATG AAGAAAGGGA ACGGGCAAGT CTCCAGCTCA ACAACCGCAG GCTGGAGCGG 
AAAGTTAAGG AGTTAATGAT GCAAGTAGAT GACGAGCACC TTACTCTAAC GGATCAGAAA 
GATCAGTTAA GTTTAAGACT GAAATCAATG AAGAGGCAAG TTGAAGAAGC TGAAGAGGAA 
ATAGACAGAC TGGAGAGCGC TAAAAAGAAA CTGCAGAGGG ATCTAGAAGA GCAAACTGAT 
ATAAACGACC AACTTCAAAC TCAACTTACT GCTGCTAAAA AGGAACTTAG ACGTAAGAGT 
TCTCCTAGCC GAATGAATGA TCTTGAGGAT GATGATGACG ACCTGAGCAC TGACGGGGAG 
AGTCTTTATG ATGGTCCATC AGTATATACG TTTTCTAGGG ATGATGGTAT AAGGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947908.1
CDS	1483..4002
Translation	MPRSKVYGSLQNQKEKNTNADEPDSISYLSSTLPSRGKKYDDVKMIKSTLQLKDCISSPPREGIRRAAATMQGSSQVAQATPDLLKGQQQVALQTNEETAKQILFNYLKEGSPDNDDATKRKVNLVFEKIQTLKSRAANTVQDPSADIKVLLEQKKELEKRVAALQERLDEEIKNHKRDKEDSNTKANMKDFHDQLEQSMDENLVLRKRLGESEKELRKHLDELFELKMEQEQYQTEIRDLQEQLSEMHDELDNAKSSVDDGEREAFIEELMQMKQSFQEVLLAKEEQEDLLRKRERELTALKGALKEEVATHDQELDKLKEQYDKELKSIRDNLTAAVKNSSDIANQKDEANQLKAVFENRVKGLLQENEELKSKIENLEAKIAALRQEIKDLNEENERIKDKLSKSEKEKQQLAIALENSKDQEKEALQFARKLENQLEDTKRSLSRVSHEHQQLTERLKEEANQKEQLRNLKNEMENERWQLDKTIEKLQKEISENVEFSRMSTVELQKQFDEYKDKYRRESAEMQRQIKDKNLEVERACQTIKKLQDEVRHLEENLQDHQRAQDEALTKNHLLQQTIKDLQYELDAKTHVKDDRIRQIKSMEDKLSQLEMELDEEKNNSDLLLERVTRCREQIEQTRSELFQERAAKQDLECDKISLERQNKDLKSRIVHLENSHRSNKEGLVAQMETRIAELEERLENEERERASLQLNNRRLERKVKELMMQVDDEHLTLTDQKDQLSLRLKSMKRQVEEAEEEIDRLESAKKKLQRDLEEQTDINDQLQTQLTAAKKELRRKSSPSRMNDLEDDDDDLSTDGESLYDGPSVYTFSRDDGIRA

Target ORF information:

RefSeq Version	XM_018092419.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092419.1

ORF Insert Sequence:

ATGCCCAGGA GCAAAGTTTA TGGATCCTTG CAGAATCAGA AGGAAAAAAA CACTAATGCA 
GATGAACCAG ACAGCATATC CTACCTTTCA TCCACTTTGC CAAGCAGAGG TAAAAAGTAT 
GATGATGTAA AAATGATTAA ATCGACTCTA CAGTTAAAGG ACTGCATTTC TAGCCCACCA 
CGGGAGGGGA TAAGGAGAGC TGCAGCTACG ATGCAGGGCT CATCTCAAGT TGCTCAGGCG 
ACGCCTGACC TTTTGAAGGG ACAACAACAA GTTGCACTAC AAACAAATGA GGAAACTGCA 
AAACAAATAC TATTTAACTA TCTAAAAGAA GGGAGCCCTG ATAATGATGA TGCAACCAAA 
AGAAAGGTCA ACCTGGTGTT TGAAAAAATC CAGACTTTAA AATCAAGAGC TGCTAACACA 
GTACAGGATC CATCTGCAGA TATCAAAGTT TTACTGGAAC AGAAAAAAGA ACTGGAAAAG 
AGAGTTGCTG CTTTGCAAGA AAGACTTGAT GAAGAAATTA AGAATCACAA ACGTGACAAA 
GAAGACTCAA ATACCAAAGC AAACATGAAA GACTTCCATG ACCAGCTGGA GCAAAGCATG 
GATGAAAATC TTGTTCTTCG GAAGAGATTG GGTGAAAGTG AAAAGGAATT AAGAAAGCAT 
CTTGATGAAT TGTTTGAACT GAAGATGGAA CAAGAGCAGT ATCAGACGGA GATCAGAGAC 
CTGCAGGAAC AGTTGTCAGA AATGCACGAT GAATTGGATA ATGCCAAGAG CTCTGTTGAT 
GATGGGGAAA GGGAAGCATT TATAGAGGAG CTGATGCAAA TGAAACAAAG TTTTCAAGAG 
GTCTTGTTGG CTAAAGAAGA GCAGGAAGAC TTATTAAGGA AACGGGAGCG TGAACTTACT 
GCACTGAAAG GAGCACTGAA GGAAGAGGTA GCAACTCATG ACCAGGAACT TGATAAGCTC 
AAGGAGCAGT ATGATAAGGA GCTGAAAAGC ATCAGAGACA ATCTGACTGC AGCAGTAAAG 
AATTCCAGTG ATATAGCTAA TCAGAAGGAT GAGGCAAATC AACTGAAAGC TGTCTTTGAA 
AACAGAGTGA AAGGCCTTTT GCAAGAAAAC GAGGAGCTAA AAAGTAAAAT AGAGAACCTT 
GAGGCCAAAA TTGCTGCACT TCGCCAGGAA ATTAAAGACT TAAATGAAGA GAATGAGAGA 
ATTAAAGACA AACTTTCAAA GTCTGAGAAG GAAAAGCAGC AGTTGGCAAT TGCCTTGGAA 
AATTCTAAGG ACCAAGAAAA GGAAGCTCTG CAGTTTGCAA GAAAGCTCGA AAATCAACTT 
GAAGACACTA AGAGAAGTCT CAGCCGAGTG TCTCATGAGC ACCAGCAATT AACAGAGAGA 
TTAAAGGAAG AAGCTAACCA GAAAGAACAG CTAAGAAATC TGAAAAATGA AATGGAAAAT 
GAGCGTTGGC AGTTGGACAA GACAATTGAA AAATTACAAA AAGAGATTTC AGAAAATGTT 
GAGTTTTCAC GGATGTCCAC AGTGGAACTT CAGAAGCAGT TTGATGAATA TAAAGACAAG 
TATCGCAGGG AAAGTGCTGA AATGCAGCGG CAGATAAAAG ACAAAAATCT GGAAGTAGAA 
AGGGCTTGCC AGACTATCAA AAAACTGCAA GATGAGGTCC GCCATTTGGA GGAGAATTTG 
CAAGATCACC AGAGAGCCCA GGATGAGGCT CTAACTAAGA ATCATCTTCT TCAGCAGACC 
ATTAAAGACC TTCAGTATGA ACTGGATGCT AAAACTCATG TGAAGGACGA TCGGATTAGG 
CAGATCAAAT CAATGGAGGA CAAGTTATCG CAGCTAGAAA TGGAACTGGA TGAAGAGAAG 
AACAATTCAG ACCTTCTGCT AGAGAGAGTA ACCAGATGCA GAGAGCAGAT TGAGCAAACG 
AGAAGTGAAC TTTTTCAAGA AAGAGCAGCA AAACAAGATC TAGAGTGCGA TAAAATATCA 
CTGGAAAGAC AGAACAAAGA CCTGAAGAGC AGAATAGTTC ATCTTGAAAA TTCACACAGA 
TCAAATAAAG AAGGGCTTGT CGCACAGATG GAAACGAGAA TAGCAGAGCT GGAAGAACGC 
CTGGAAAATG AAGAAAGGGA ACGGGCAAGT CTCCAGCTCA ACAACCGCAG GCTGGAGCGG 
AAAGTTAAGG AGTTAATGAT GCAAGTAGAT GACGAGCACC TTACTCTAAC GGATCAGAAA 
GATCAGTTAA GTTTAAGACT GAAATCAATG AAGAGGCAAG TTGAAGAAGC TGAAGAGGAA 
ATAGACAGAC TGGAGAGCGC TAAAAAGAAA CTGCAGAGGG ATCTAGAAGA GCAAACTGAT 
ATAAACGACC AACTTCAAAC TCAACTTACT GCTGCTAAAA AGGAACTTAG ACGTAAGAGT 
TCTCCTAGCC GAATGAATGA TCTTGAGGAT GATGATGACG ACCTGAGCAC TGACGGGGAG 
AGTCTTTATG ATGGTCCATC AGTATATACG TTTTCTAGGG ATGATGGTAT AAGGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cgnl1 ( XM_018092417.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947906.2 Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa55067
Clone ID Related Accession (Same CDS sequence)	XM_018092417.2 , XM_018092418.2
Accession Version	XM_018092417.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cingulin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092417.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCAC GCTTTGATGG TTTAAGATAT ATTCAACAGG ACTGTGGTGT TCTCCACCAA 
AGAAGTGATG AACGGCCTCA AAAGCTTAGA AATTCACACA ATATTAAAGA AGGCACTTAT 
GGTGTAAGTA TTAGGGTCCA GGGAATAGAT GGTCACCCCT ACATTGTTTT AAATAATAAA 
GAAGGACATT TGTCAGAAAA TGACTACTCA TCAGACCGTT GTTCCCAGTC CTTAAAAAGG 
CCTGCAGTTA TATCCAGTGA AAACTCATTT CCTGAAAATC CATTTGAAGC ATCTGTTCGC 
AATACAAATA AAACACAGTA TATAAATTCT GGAAATGGAT TCAAAGGTAG AACTGTAAAA 
CAATCAAACT TGCTAAATTT CCAGAAGCAT CCAGAACTTT TAAAACCATA TGATCCAGAT 
GAACACAGAC GAAATGAAGA CAATTTGCCC AATAATCAAT GCAAAATACC TGCTGTAGAG 
GAACCAAAGA AAAATCATAA ACTTTCTGGT TCCTGTAATA AGGGGATTTC AGCTACATAT 
TCGAGTATCA AATATGTGGA TACACCAAAA TACGAAGACA AGGAAGCATC ACTTAATCAA 
ACAACTAAGC AAGACATCCA AAGCAAAATA TCAGTGGATA ACAAAAGCAT TTTAAGCAGC 
CCAGGTGATA CTTCTACCTC AGAGTCTTTC TCATCAAGTA ACGGTTCACT GTCTAGTAAA 
CACTCAACTG CTTATGTGGA GGCCAAAAAA TCAAGGCCAG ATGTTCTTCA GTTTAGGAGA 
CAGGATTCTG CAGGTGCAGT TGAAGGATCG CGCTCACGTA GATCATCTGG ATCCTCAACT 
GCAACTTCTC AGTCTTTTCA GAAATTTTTG CTCGATGAAC AAGAAGATGC TTTGTATGCA 
GATAATGTGA ACCGTCATGA AAACAGAAGG TATATTCCTT TCGTACCAGG AACAGGTCGT 
GACATTGACA CTGACTGTAT TTTGGGAGTA GATGAGTTGA TTGAAAAGTT TGATAGGAAT 
ATTTCCATGC AGAGGAGAGG TAGGTCTGCC AAACGAAACA GAATTCATCC AGAAGACCGC 
AAAAGGGCAA GAAGTGTTGA CAGTGCATTT CCATTTGGCC TTCAAAGTAA TTCAGAATAT 
TTAAATGAGT TCAGTAAAAG TTTGGGAAAG TCAAATGAAC ATTTACTGAA ACCCTCCAAA 
ATCTGCCCGC AAAAATCACT CACTCAGTAC TACAAAATGT CCTTTAAGAG ACAGAACACA 
ACAGGGAGCC TTGTTTCTCA GCCCTCTCTG CTTGATAATG CCCAGAGCAA AGTTTATGGA 
TCCTTGCAGA ATCAGAAGGA AAAAAACACT AATGCAGATG AACCAGACAG CATATCCTAC 
CTTTCATCCA CTTTGCCAAG CAGAGGTAAA AAGTATGATG ATGTAAAAAT GATTAAATCG 
ACTCTACAGT TAAAGGACTG CATTTCTAGC CCACCACGGG AGGGGATAAG GAGAGCTGCA 
GCTACGATGC AGGGCTCATC TCAAGTTGCT CAGGCGACGC CTGACCTTTT GAAGGGACAA 
CAACAAGTTG CACTACAAAC AAATGAGGAA ACTGCAAAAC AAATACTATT TAACTATCTA 
AAAGAAGGGA GCCCTGATAA TGATGATGCA ACCAAAAGAA AGGTCAACCT GGTGTTTGAA 
AAAATCCAGA CTTTAAAATC AAGAGCTGCT AACACAGTAC AGGATCCATC TGCAGATATC 
AAAGTTTTAC TGGAACAGAA AAAAGAACTG GAAAAGAGAG TTGCTGCTTT GCAAGAAAGA 
CTTGATGAAG AAATTAAGAA TCACAAACGT GACAAAGAAG ACTCAAATAC CAAAGCAAAC 
ATGAAAGACT TCCATGACCA GCTGGAGCAA AGCATGGATG AAAATCTTGT TCTTCGGAAG 
AGATTGGGTG AAAGTGAAAA GGAATTAAGA AAGCATCTTG ATGAATTGTT TGAACTGAAG 
ATGGAACAAG AGCAGTATCA GACGGAGATC AGAGACCTGC AGGAACAGTT GTCAGAAATG 
CACGATGAAT TGGATAATGC CAAGAGCTCT GTTGATGATG GGGAAAGGGA AGCATTTATA 
GAGGAGCTGA TGCAAATGAA ACAAAGTTTT CAAGAGGTCT TGTTGGCTAA AGAAGAGCAG 
GAAGACTTAT TAAGGAAACG GGAGCGTGAA CTTACTGCAC TGAAAGGAGC ACTGAAGGAA 
GAGGTAGCAA CTCATGACCA GGAACTTGAT AAGCTCAAGG AGCAGTATGA TAAGGAGCTG 
AAAAGCATCA GAGACAATCT GACTGCAGCA GTAAAGAATT CCAGTGATAT AGCTAATCAG 
AAGGATGAGG CAAATCAACT GAAAGCTGTC TTTGAAAACA GAGTGAAAGG CCTTTTGCAA 
GAAAACGAGG AGCTAAAAAG TAAAATAGAG AACCTTGAGG CCAAAATTGC TGCACTTCGC 
CAGGAAATTA AAGACTTAAA TGAAGAGAAT GAGAGAATTA AAGACAAACT TTCAAAGTCT 
GAGAAGGAAA AGCAGCAGTT GGCAATTGCC TTGGAAAATT CTAAGGACCA AGAAAAGGAA 
GCTCTGCAGT TTGCAAGAAA GCTCGAAAAT CAACTTGAAG ACACTAAGAG AAGTCTCAGC 
CGAGTGTCTC ATGAGCACCA GCAATTAACA GAGAGATTAA AGGAAGAAGC TAACCAGAAA 
GAACAGCTAA GGAATCTGAA AAATGAAATG GAAAATGAGC GTTGGCAGTT GGACAAGACA 
ATTGAAAAAT TACAAAAAGA GATTTCAGAA AATGTTGAGT TTTCACGGAT GTCCACAGTG 
GAACTTCAGA AGCAGTTTGA TGAATATAAA GACAAGTATC GCAGGGAAAG TGCTGAAATG 
CAGCGGCAGA TAAAAGACAA AAATCTGGAA GTAGAAAGGG CTTGCCAGAC TATCAAAAAA 
CTGCAAGATG AGGTCCGCCA TTTGGAGGAG AATTTGCAAG ATCACCAGAG AGCCCAGGAT 
GAGGCTCTAA CTAAGAATCA TCTTCTTCAG CAGACCATTA AAGACCTTCA GTATGAACTG 
GATGCTAAAA CTCATGTGAA GGACGATCGG ATTAGGCAGA TCAAATCAAT GGAGGACAAG 
TTATCGCAGC TAGAAATGGA ACTGGATGAA GAGAAGAACA ATTCAGACCT TCTGCTAGAG 
AGAGTAACCA GATGCAGAGA GCAGATTGAG CAAACGAGAA GTGAACTATT TCAAGAAAGA 
GCAGCAAAAC AAGATCTAGA GTGCGATAAA ATATCACTGG AAAGACAGAA CAAAGACCTG 
AAGAGCAGAA TAGTTCATCT TGAAAATTCA CACAGATCAA ATAAAGAAGG GCTTGTCGCA 
CAGATGGAAA CGAGAATAGC AGAGCTGGAA GAACGCCTGG AAAATGAAGA AAGGGAACGG 
GCAAGTCTCC AGCTCAACAA CCGCAGGCTG GAGCGGAAAG TTAAGGAGTT AATGATGCAA 
GTAGATGACG AGCACCTTAC TCTAACGGAT CAGAAAGATC AGTTAAGTTT AAGACTGAAA 
TCAATGAAGA GGCAAGTTGA AGAAGCTGAA GAGGAAATAG ACAGACTGGA GAGCGCTAAA 
AAGAAACTGC AGAGGGATCT AGAAGAGCAA ACTGATATAA ACGACCAACT TCAAACTCAA 
CTTACTGCTG CTAAAAAGGA ACTTAGCAGA CGTAAGAGTT CTCCTAGCCG AATGAATGAT 
CTTGAGGATG ATGATGACGA CCTGAGCACT GACGGGGAGA GTCTTTATGA TGGTCCATCA 
GTATATACGT TTTCTAGGGA TGATGGTATA AGGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947906.2
CDS	214..4032
Translation	MDSRFDGLRYIQQDCGVLHQRSDERPQKLRNSHNIKEGTYGVSIRVQGIDGHPYIVLNNKEGHLSENDYSSDRCSQSLKRPAVISSENSFPENPFEASVRNTNKTQYINSGNGFKGRTVKQSNLLNFQKHPELLKPYDPDEHRRNEDNLPNNQCKIPAVEEPKKNHKLSGSCNKGISATYSSIKYVDTPKYEDKEASLNQTTKQDIQSKISVDNKSILSSPGDTSTSESFSSSNGSLSSKHSTAYVEAKKSRPDVLQFRRQDSAGAVEGSRSRRSSGSSTATSQSFQKFLLDEQEDALYADNVNRHENRRYIPFVPGTGRDIDTDCILGVDELIEKFDRNISMQRRGRSAKRNRIHPEDRKRARSVDSAFPFGLQSNSEYLNEFSKSLGKSNEHLLKPSKICPQKSLTQYYKMSFKRQNTTGSLVSQPSLLDNAQSKVYGSLQNQKEKNTNADEPDSISYLSSTLPSRGKKYDDVKMIKSTLQLKDCISSPPREGIRRAAATMQGSSQVAQATPDLLKGQQQVALQTNEETAKQILFNYLKEGSPDNDDATKRKVNLVFEKIQTLKSRAANTVQDPSADIKVLLEQKKELEKRVAALQERLDEEIKNHKRDKEDSNTKANMKDFHDQLEQSMDENLVLRKRLGESEKELRKHLDELFELKMEQEQYQTEIRDLQEQLSEMHDELDNAKSSVDDGEREAFIEELMQMKQSFQEVLLAKEEQEDLLRKRERELTALKGALKEEVATHDQELDKLKEQYDKELKSIRDNLTAAVKNSSDIANQKDEANQLKAVFENRVKGLLQENEELKSKIENLEAKIAALRQEIKDLNEENERIKDKLSKSEKEKQQLAIALENSKDQEKEALQFARKLENQLEDTKRSLSRVSHEHQQLTERLKEEANQKEQLRNLKNEMENERWQLDKTIEKLQKEISENVEFSRMSTVELQKQFDEYKDKYRRESAEMQRQIKDKNLEVERACQTIKKLQDEVRHLEENLQDHQRAQDEALTKNHLLQQTIKDLQYELDAKTHVKDDRIRQIKSMEDKLSQLEMELDEEKNNSDLLLERVTRCREQIEQTRSELFQERAAKQDLECDKISLERQNKDLKSRIVHLENSHRSNKEGLVAQMETRIAELEERLENEERERASLQLNNRRLERKVKELMMQVDDEHLTLTDQKDQLSLRLKSMKRQVEEAEEEIDRLESAKKKLQRDLEEQTDINDQLQTQLTAAKKELSRRKSSPSRMNDLEDDDDDLSTDGESLYDGPSVYTFSRDDGIRA

Target ORF information:

RefSeq Version	XM_018092417.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092417.2

ORF Insert Sequence:

ATGGATTCAC GCTTTGATGG TTTAAGATAT ATTCAACAGG ACTGTGGTGT TCTCCACCAA 
AGAAGTGATG AACGGCCTCA AAAGCTTAGA AATTCACACA ATATTAAAGA AGGCACTTAT 
GGTGTAAGTA TTAGGGTCCA GGGAATAGAT GGTCACCCCT ACATTGTTTT AAATAATAAA 
GAAGGACATT TGTCAGAAAA TGACTACTCA TCAGACCGTT GTTCCCAGTC CTTAAAAAGG 
CCTGCAGTTA TATCCAGTGA AAACTCATTT CCTGAAAATC CATTTGAAGC ATCTGTTCGC 
AATACAAATA AAACACAGTA TATAAATTCT GGAAATGGAT TCAAAGGTAG AACTGTAAAA 
CAATCAAACT TGCTAAATTT CCAGAAGCAT CCAGAACTTT TAAAACCATA TGATCCAGAT 
GAACACAGAC GAAATGAAGA CAATTTGCCC AATAATCAAT GCAAAATACC TGCTGTAGAG 
GAACCAAAGA AAAATCATAA ACTTTCTGGT TCCTGTAATA AGGGGATTTC AGCTACATAT 
TCGAGTATCA AATATGTGGA TACACCAAAA TACGAAGACA AGGAAGCATC ACTTAATCAA 
ACAACTAAGC AAGACATCCA AAGCAAAATA TCAGTGGATA ACAAAAGCAT TTTAAGCAGC 
CCAGGTGATA CTTCTACCTC AGAGTCTTTC TCATCAAGTA ACGGTTCACT GTCTAGTAAA 
CACTCAACTG CTTATGTGGA GGCCAAAAAA TCAAGGCCAG ATGTTCTTCA GTTTAGGAGA 
CAGGATTCTG CAGGTGCAGT TGAAGGATCG CGCTCACGTA GATCATCTGG ATCCTCAACT 
GCAACTTCTC AGTCTTTTCA GAAATTTTTG CTCGATGAAC AAGAAGATGC TTTGTATGCA 
GATAATGTGA ACCGTCATGA AAACAGAAGG TATATTCCTT TCGTACCAGG AACAGGTCGT 
GACATTGACA CTGACTGTAT TTTGGGAGTA GATGAGTTGA TTGAAAAGTT TGATAGGAAT 
ATTTCCATGC AGAGGAGAGG TAGGTCTGCC AAACGAAACA GAATTCATCC AGAAGACCGC 
AAAAGGGCAA GAAGTGTTGA CAGTGCATTT CCATTTGGCC TTCAAAGTAA TTCAGAATAT 
TTAAATGAGT TCAGTAAAAG TTTGGGAAAG TCAAATGAAC ATTTACTGAA ACCCTCCAAA 
ATCTGCCCGC AAAAATCACT CACTCAGTAC TACAAAATGT CCTTTAAGAG ACAGAACACA 
ACAGGGAGCC TTGTTTCTCA GCCCTCTCTG CTTGATAATG CCCAGAGCAA AGTTTATGGA 
TCCTTGCAGA ATCAGAAGGA AAAAAACACT AATGCAGATG AACCAGACAG CATATCCTAC 
CTTTCATCCA CTTTGCCAAG CAGAGGTAAA AAGTATGATG ATGTAAAAAT GATTAAATCG 
ACTCTACAGT TAAAGGACTG CATTTCTAGC CCACCACGGG AGGGGATAAG GAGAGCTGCA 
GCTACGATGC AGGGCTCATC TCAAGTTGCT CAGGCGACGC CTGACCTTTT GAAGGGACAA 
CAACAAGTTG CACTACAAAC AAATGAGGAA ACTGCAAAAC AAATACTATT TAACTATCTA 
AAAGAAGGGA GCCCTGATAA TGATGATGCA ACCAAAAGAA AGGTCAACCT GGTGTTTGAA 
AAAATCCAGA CTTTAAAATC AAGAGCTGCT AACACAGTAC AGGATCCATC TGCAGATATC 
AAAGTTTTAC TGGAACAGAA AAAAGAACTG GAAAAGAGAG TTGCTGCTTT GCAAGAAAGA 
CTTGATGAAG AAATTAAGAA TCACAAACGT GACAAAGAAG ACTCAAATAC CAAAGCAAAC 
ATGAAAGACT TCCATGACCA GCTGGAGCAA AGCATGGATG AAAATCTTGT TCTTCGGAAG 
AGATTGGGTG AAAGTGAAAA GGAATTAAGA AAGCATCTTG ATGAATTGTT TGAACTGAAG 
ATGGAACAAG AGCAGTATCA GACGGAGATC AGAGACCTGC AGGAACAGTT GTCAGAAATG 
CACGATGAAT TGGATAATGC CAAGAGCTCT GTTGATGATG GGGAAAGGGA AGCATTTATA 
GAGGAGCTGA TGCAAATGAA ACAAAGTTTT CAAGAGGTCT TGTTGGCTAA AGAAGAGCAG 
GAAGACTTAT TAAGGAAACG GGAGCGTGAA CTTACTGCAC TGAAAGGAGC ACTGAAGGAA 
GAGGTAGCAA CTCATGACCA GGAACTTGAT AAGCTCAAGG AGCAGTATGA TAAGGAGCTG 
AAAAGCATCA GAGACAATCT GACTGCAGCA GTAAAGAATT CCAGTGATAT AGCTAATCAG 
AAGGATGAGG CAAATCAACT GAAAGCTGTC TTTGAAAACA GAGTGAAAGG CCTTTTGCAA 
GAAAACGAGG AGCTAAAAAG TAAAATAGAG AACCTTGAGG CCAAAATTGC TGCACTTCGC 
CAGGAAATTA AAGACTTAAA TGAAGAGAAT GAGAGAATTA AAGACAAACT TTCAAAGTCT 
GAGAAGGAAA AGCAGCAGTT GGCAATTGCC TTGGAAAATT CTAAGGACCA AGAAAAGGAA 
GCTCTGCAGT TTGCAAGAAA GCTCGAAAAT CAACTTGAAG ACACTAAGAG AAGTCTCAGC 
CGAGTGTCTC ATGAGCACCA GCAATTAACA GAGAGATTAA AGGAAGAAGC TAACCAGAAA 
GAACAGCTAA GGAATCTGAA AAATGAAATG GAAAATGAGC GTTGGCAGTT GGACAAGACA 
ATTGAAAAAT TACAAAAAGA GATTTCAGAA AATGTTGAGT TTTCACGGAT GTCCACAGTG 
GAACTTCAGA AGCAGTTTGA TGAATATAAA GACAAGTATC GCAGGGAAAG TGCTGAAATG 
CAGCGGCAGA TAAAAGACAA AAATCTGGAA GTAGAAAGGG CTTGCCAGAC TATCAAAAAA 
CTGCAAGATG AGGTCCGCCA TTTGGAGGAG AATTTGCAAG ATCACCAGAG AGCCCAGGAT 
GAGGCTCTAA CTAAGAATCA TCTTCTTCAG CAGACCATTA AAGACCTTCA GTATGAACTG 
GATGCTAAAA CTCATGTGAA GGACGATCGG ATTAGGCAGA TCAAATCAAT GGAGGACAAG 
TTATCGCAGC TAGAAATGGA ACTGGATGAA GAGAAGAACA ATTCAGACCT TCTGCTAGAG 
AGAGTAACCA GATGCAGAGA GCAGATTGAG CAAACGAGAA GTGAACTATT TCAAGAAAGA 
GCAGCAAAAC AAGATCTAGA GTGCGATAAA ATATCACTGG AAAGACAGAA CAAAGACCTG 
AAGAGCAGAA TAGTTCATCT TGAAAATTCA CACAGATCAA ATAAAGAAGG GCTTGTCGCA 
CAGATGGAAA CGAGAATAGC AGAGCTGGAA GAACGCCTGG AAAATGAAGA AAGGGAACGG 
GCAAGTCTCC AGCTCAACAA CCGCAGGCTG GAGCGGAAAG TTAAGGAGTT AATGATGCAA 
GTAGATGACG AGCACCTTAC TCTAACGGAT CAGAAAGATC AGTTAAGTTT AAGACTGAAA 
TCAATGAAGA GGCAAGTTGA AGAAGCTGAA GAGGAAATAG ACAGACTGGA GAGCGCTAAA 
AAGAAACTGC AGAGGGATCT AGAAGAGCAA ACTGATATAA ACGACCAACT TCAAACTCAA 
CTTACTGCTG CTAAAAAGGA ACTTAGCAGA CGTAAGAGTT CTCCTAGCCG AATGAATGAT 
CTTGAGGATG ATGATGACGA CCTGAGCACT GACGGGGAGA GTCTTTATGA TGGTCCATCA 
GTATATACGT TTTCTAGGGA TGATGGTATA AGGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cgnl1 ( XM_018092418.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947907.2 Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa55067
Clone ID Related Accession (Same CDS sequence)	XM_018092417.2 , XM_018092418.2
Accession Version	XM_018092418.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cingulin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCAC GCTTTGATGG TTTAAGATAT ATTCAACAGG ACTGTGGTGT TCTCCACCAA 
AGAAGTGATG AACGGCCTCA AAAGCTTAGA AATTCACACA ATATTAAAGA AGGCACTTAT 
GGTGTAAGTA TTAGGGTCCA GGGAATAGAT GGTCACCCCT ACATTGTTTT AAATAATAAA 
GAAGGACATT TGTCAGAAAA TGACTACTCA TCAGACCGTT GTTCCCAGTC CTTAAAAAGG 
CCTGCAGTTA TATCCAGTGA AAACTCATTT CCTGAAAATC CATTTGAAGC ATCTGTTCGC 
AATACAAATA AAACACAGTA TATAAATTCT GGAAATGGAT TCAAAGGTAG AACTGTAAAA 
CAATCAAACT TGCTAAATTT CCAGAAGCAT CCAGAACTTT TAAAACCATA TGATCCAGAT 
GAACACAGAC GAAATGAAGA CAATTTGCCC AATAATCAAT GCAAAATACC TGCTGTAGAG 
GAACCAAAGA AAAATCATAA ACTTTCTGGT TCCTGTAATA AGGGGATTTC AGCTACATAT 
TCGAGTATCA AATATGTGGA TACACCAAAA TACGAAGACA AGGAAGCATC ACTTAATCAA 
ACAACTAAGC AAGACATCCA AAGCAAAATA TCAGTGGATA ACAAAAGCAT TTTAAGCAGC 
CCAGGTGATA CTTCTACCTC AGAGTCTTTC TCATCAAGTA ACGGTTCACT GTCTAGTAAA 
CACTCAACTG CTTATGTGGA GGCCAAAAAA TCAAGGCCAG ATGTTCTTCA GTTTAGGAGA 
CAGGATTCTG CAGGTGCAGT TGAAGGATCG CGCTCACGTA GATCATCTGG ATCCTCAACT 
GCAACTTCTC AGTCTTTTCA GAAATTTTTG CTCGATGAAC AAGAAGATGC TTTGTATGCA 
GATAATGTGA ACCGTCATGA AAACAGAAGG TATATTCCTT TCGTACCAGG AACAGGTCGT 
GACATTGACA CTGACTGTAT TTTGGGAGTA GATGAGTTGA TTGAAAAGTT TGATAGGAAT 
ATTTCCATGC AGAGGAGAGG TAGGTCTGCC AAACGAAACA GAATTCATCC AGAAGACCGC 
AAAAGGGCAA GAAGTGTTGA CAGTGCATTT CCATTTGGCC TTCAAAGTAA TTCAGAATAT 
TTAAATGAGT TCAGTAAAAG TTTGGGAAAG TCAAATGAAC ATTTACTGAA ACCCTCCAAA 
ATCTGCCCGC AAAAATCACT CACTCAGTAC TACAAAATGT CCTTTAAGAG ACAGAACACA 
ACAGGGAGCC TTGTTTCTCA GCCCTCTCTG CTTGATAATG CCCAGAGCAA AGTTTATGGA 
TCCTTGCAGA ATCAGAAGGA AAAAAACACT AATGCAGATG AACCAGACAG CATATCCTAC 
CTTTCATCCA CTTTGCCAAG CAGAGGTAAA AAGTATGATG ATGTAAAAAT GATTAAATCG 
ACTCTACAGT TAAAGGACTG CATTTCTAGC CCACCACGGG AGGGGATAAG GAGAGCTGCA 
GCTACGATGC AGGGCTCATC TCAAGTTGCT CAGGCGACGC CTGACCTTTT GAAGGGACAA 
CAACAAGTTG CACTACAAAC AAATGAGGAA ACTGCAAAAC AAATACTATT TAACTATCTA 
AAAGAAGGGA GCCCTGATAA TGATGATGCA ACCAAAAGAA AGGTCAACCT GGTGTTTGAA 
AAAATCCAGA CTTTAAAATC AAGAGCTGCT AACACAGTAC AGGATCCATC TGCAGATATC 
AAAGTTTTAC TGGAACAGAA AAAAGAACTG GAAAAGAGAG TTGCTGCTTT GCAAGAAAGA 
CTTGATGAAG AAATTAAGAA TCACAAACGT GACAAAGAAG ACTCAAATAC CAAAGCAAAC 
ATGAAAGACT TCCATGACCA GCTGGAGCAA AGCATGGATG AAAATCTTGT TCTTCGGAAG 
AGATTGGGTG AAAGTGAAAA GGAATTAAGA AAGCATCTTG ATGAATTGTT TGAACTGAAG 
ATGGAACAAG AGCAGTATCA GACGGAGATC AGAGACCTGC AGGAACAGTT GTCAGAAATG 
CACGATGAAT TGGATAATGC CAAGAGCTCT GTTGATGATG GGGAAAGGGA AGCATTTATA 
GAGGAGCTGA TGCAAATGAA ACAAAGTTTT CAAGAGGTCT TGTTGGCTAA AGAAGAGCAG 
GAAGACTTAT TAAGGAAACG GGAGCGTGAA CTTACTGCAC TGAAAGGAGC ACTGAAGGAA 
GAGGTAGCAA CTCATGACCA GGAACTTGAT AAGCTCAAGG AGCAGTATGA TAAGGAGCTG 
AAAAGCATCA GAGACAATCT GACTGCAGCA GTAAAGAATT CCAGTGATAT AGCTAATCAG 
AAGGATGAGG CAAATCAACT GAAAGCTGTC TTTGAAAACA GAGTGAAAGG CCTTTTGCAA 
GAAAACGAGG AGCTAAAAAG TAAAATAGAG AACCTTGAGG CCAAAATTGC TGCACTTCGC 
CAGGAAATTA AAGACTTAAA TGAAGAGAAT GAGAGAATTA AAGACAAACT TTCAAAGTCT 
GAGAAGGAAA AGCAGCAGTT GGCAATTGCC TTGGAAAATT CTAAGGACCA AGAAAAGGAA 
GCTCTGCAGT TTGCAAGAAA GCTCGAAAAT CAACTTGAAG ACACTAAGAG AAGTCTCAGC 
CGAGTGTCTC ATGAGCACCA GCAATTAACA GAGAGATTAA AGGAAGAAGC TAACCAGAAA 
GAACAGCTAA GGAATCTGAA AAATGAAATG GAAAATGAGC GTTGGCAGTT GGACAAGACA 
ATTGAAAAAT TACAAAAAGA GATTTCAGAA AATGTTGAGT TTTCACGGAT GTCCACAGTG 
GAACTTCAGA AGCAGTTTGA TGAATATAAA GACAAGTATC GCAGGGAAAG TGCTGAAATG 
CAGCGGCAGA TAAAAGACAA AAATCTGGAA GTAGAAAGGG CTTGCCAGAC TATCAAAAAA 
CTGCAAGATG AGGTCCGCCA TTTGGAGGAG AATTTGCAAG ATCACCAGAG AGCCCAGGAT 
GAGGCTCTAA CTAAGAATCA TCTTCTTCAG CAGACCATTA AAGACCTTCA GTATGAACTG 
GATGCTAAAA CTCATGTGAA GGACGATCGG ATTAGGCAGA TCAAATCAAT GGAGGACAAG 
TTATCGCAGC TAGAAATGGA ACTGGATGAA GAGAAGAACA ATTCAGACCT TCTGCTAGAG 
AGAGTAACCA GATGCAGAGA GCAGATTGAG CAAACGAGAA GTGAACTATT TCAAGAAAGA 
GCAGCAAAAC AAGATCTAGA GTGCGATAAA ATATCACTGG AAAGACAGAA CAAAGACCTG 
AAGAGCAGAA TAGTTCATCT TGAAAATTCA CACAGATCAA ATAAAGAAGG GCTTGTCGCA 
CAGATGGAAA CGAGAATAGC AGAGCTGGAA GAACGCCTGG AAAATGAAGA AAGGGAACGG 
GCAAGTCTCC AGCTCAACAA CCGCAGGCTG GAGCGGAAAG TTAAGGAGTT AATGATGCAA 
GTAGATGACG AGCACCTTAC TCTAACGGAT CAGAAAGATC AGTTAAGTTT AAGACTGAAA 
TCAATGAAGA GGCAAGTTGA AGAAGCTGAA GAGGAAATAG ACAGACTGGA GAGCGCTAAA 
AAGAAACTGC AGAGGGATCT AGAAGAGCAA ACTGATATAA ACGACCAACT TCAAACTCAA 
CTTACTGCTG CTAAAAAGGA ACTTAGCAGA CGTAAGAGTT CTCCTAGCCG AATGAATGAT 
CTTGAGGATG ATGATGACGA CCTGAGCACT GACGGGGAGA GTCTTTATGA TGGTCCATCA 
GTATATACGT TTTCTAGGGA TGATGGTATA AGGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947907.2
CDS	44..3862
Translation	MDSRFDGLRYIQQDCGVLHQRSDERPQKLRNSHNIKEGTYGVSIRVQGIDGHPYIVLNNKEGHLSENDYSSDRCSQSLKRPAVISSENSFPENPFEASVRNTNKTQYINSGNGFKGRTVKQSNLLNFQKHPELLKPYDPDEHRRNEDNLPNNQCKIPAVEEPKKNHKLSGSCNKGISATYSSIKYVDTPKYEDKEASLNQTTKQDIQSKISVDNKSILSSPGDTSTSESFSSSNGSLSSKHSTAYVEAKKSRPDVLQFRRQDSAGAVEGSRSRRSSGSSTATSQSFQKFLLDEQEDALYADNVNRHENRRYIPFVPGTGRDIDTDCILGVDELIEKFDRNISMQRRGRSAKRNRIHPEDRKRARSVDSAFPFGLQSNSEYLNEFSKSLGKSNEHLLKPSKICPQKSLTQYYKMSFKRQNTTGSLVSQPSLLDNAQSKVYGSLQNQKEKNTNADEPDSISYLSSTLPSRGKKYDDVKMIKSTLQLKDCISSPPREGIRRAAATMQGSSQVAQATPDLLKGQQQVALQTNEETAKQILFNYLKEGSPDNDDATKRKVNLVFEKIQTLKSRAANTVQDPSADIKVLLEQKKELEKRVAALQERLDEEIKNHKRDKEDSNTKANMKDFHDQLEQSMDENLVLRKRLGESEKELRKHLDELFELKMEQEQYQTEIRDLQEQLSEMHDELDNAKSSVDDGEREAFIEELMQMKQSFQEVLLAKEEQEDLLRKRERELTALKGALKEEVATHDQELDKLKEQYDKELKSIRDNLTAAVKNSSDIANQKDEANQLKAVFENRVKGLLQENEELKSKIENLEAKIAALRQEIKDLNEENERIKDKLSKSEKEKQQLAIALENSKDQEKEALQFARKLENQLEDTKRSLSRVSHEHQQLTERLKEEANQKEQLRNLKNEMENERWQLDKTIEKLQKEISENVEFSRMSTVELQKQFDEYKDKYRRESAEMQRQIKDKNLEVERACQTIKKLQDEVRHLEENLQDHQRAQDEALTKNHLLQQTIKDLQYELDAKTHVKDDRIRQIKSMEDKLSQLEMELDEEKNNSDLLLERVTRCREQIEQTRSELFQERAAKQDLECDKISLERQNKDLKSRIVHLENSHRSNKEGLVAQMETRIAELEERLENEERERASLQLNNRRLERKVKELMMQVDDEHLTLTDQKDQLSLRLKSMKRQVEEAEEEIDRLESAKKKLQRDLEEQTDINDQLQTQLTAAKKELSRRKSSPSRMNDLEDDDDDLSTDGESLYDGPSVYTFSRDDGIRA

Target ORF information:

RefSeq Version	XM_018092418.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092418.2

ORF Insert Sequence:

ATGGATTCAC GCTTTGATGG TTTAAGATAT ATTCAACAGG ACTGTGGTGT TCTCCACCAA 
AGAAGTGATG AACGGCCTCA AAAGCTTAGA AATTCACACA ATATTAAAGA AGGCACTTAT 
GGTGTAAGTA TTAGGGTCCA GGGAATAGAT GGTCACCCCT ACATTGTTTT AAATAATAAA 
GAAGGACATT TGTCAGAAAA TGACTACTCA TCAGACCGTT GTTCCCAGTC CTTAAAAAGG 
CCTGCAGTTA TATCCAGTGA AAACTCATTT CCTGAAAATC CATTTGAAGC ATCTGTTCGC 
AATACAAATA AAACACAGTA TATAAATTCT GGAAATGGAT TCAAAGGTAG AACTGTAAAA 
CAATCAAACT TGCTAAATTT CCAGAAGCAT CCAGAACTTT TAAAACCATA TGATCCAGAT 
GAACACAGAC GAAATGAAGA CAATTTGCCC AATAATCAAT GCAAAATACC TGCTGTAGAG 
GAACCAAAGA AAAATCATAA ACTTTCTGGT TCCTGTAATA AGGGGATTTC AGCTACATAT 
TCGAGTATCA AATATGTGGA TACACCAAAA TACGAAGACA AGGAAGCATC ACTTAATCAA 
ACAACTAAGC AAGACATCCA AAGCAAAATA TCAGTGGATA ACAAAAGCAT TTTAAGCAGC 
CCAGGTGATA CTTCTACCTC AGAGTCTTTC TCATCAAGTA ACGGTTCACT GTCTAGTAAA 
CACTCAACTG CTTATGTGGA GGCCAAAAAA TCAAGGCCAG ATGTTCTTCA GTTTAGGAGA 
CAGGATTCTG CAGGTGCAGT TGAAGGATCG CGCTCACGTA GATCATCTGG ATCCTCAACT 
GCAACTTCTC AGTCTTTTCA GAAATTTTTG CTCGATGAAC AAGAAGATGC TTTGTATGCA 
GATAATGTGA ACCGTCATGA AAACAGAAGG TATATTCCTT TCGTACCAGG AACAGGTCGT 
GACATTGACA CTGACTGTAT TTTGGGAGTA GATGAGTTGA TTGAAAAGTT TGATAGGAAT 
ATTTCCATGC AGAGGAGAGG TAGGTCTGCC AAACGAAACA GAATTCATCC AGAAGACCGC 
AAAAGGGCAA GAAGTGTTGA CAGTGCATTT CCATTTGGCC TTCAAAGTAA TTCAGAATAT 
TTAAATGAGT TCAGTAAAAG TTTGGGAAAG TCAAATGAAC ATTTACTGAA ACCCTCCAAA 
ATCTGCCCGC AAAAATCACT CACTCAGTAC TACAAAATGT CCTTTAAGAG ACAGAACACA 
ACAGGGAGCC TTGTTTCTCA GCCCTCTCTG CTTGATAATG CCCAGAGCAA AGTTTATGGA 
TCCTTGCAGA ATCAGAAGGA AAAAAACACT AATGCAGATG AACCAGACAG CATATCCTAC 
CTTTCATCCA CTTTGCCAAG CAGAGGTAAA AAGTATGATG ATGTAAAAAT GATTAAATCG 
ACTCTACAGT TAAAGGACTG CATTTCTAGC CCACCACGGG AGGGGATAAG GAGAGCTGCA 
GCTACGATGC AGGGCTCATC TCAAGTTGCT CAGGCGACGC CTGACCTTTT GAAGGGACAA 
CAACAAGTTG CACTACAAAC AAATGAGGAA ACTGCAAAAC AAATACTATT TAACTATCTA 
AAAGAAGGGA GCCCTGATAA TGATGATGCA ACCAAAAGAA AGGTCAACCT GGTGTTTGAA 
AAAATCCAGA CTTTAAAATC AAGAGCTGCT AACACAGTAC AGGATCCATC TGCAGATATC 
AAAGTTTTAC TGGAACAGAA AAAAGAACTG GAAAAGAGAG TTGCTGCTTT GCAAGAAAGA 
CTTGATGAAG AAATTAAGAA TCACAAACGT GACAAAGAAG ACTCAAATAC CAAAGCAAAC 
ATGAAAGACT TCCATGACCA GCTGGAGCAA AGCATGGATG AAAATCTTGT TCTTCGGAAG 
AGATTGGGTG AAAGTGAAAA GGAATTAAGA AAGCATCTTG ATGAATTGTT TGAACTGAAG 
ATGGAACAAG AGCAGTATCA GACGGAGATC AGAGACCTGC AGGAACAGTT GTCAGAAATG 
CACGATGAAT TGGATAATGC CAAGAGCTCT GTTGATGATG GGGAAAGGGA AGCATTTATA 
GAGGAGCTGA TGCAAATGAA ACAAAGTTTT CAAGAGGTCT TGTTGGCTAA AGAAGAGCAG 
GAAGACTTAT TAAGGAAACG GGAGCGTGAA CTTACTGCAC TGAAAGGAGC ACTGAAGGAA 
GAGGTAGCAA CTCATGACCA GGAACTTGAT AAGCTCAAGG AGCAGTATGA TAAGGAGCTG 
AAAAGCATCA GAGACAATCT GACTGCAGCA GTAAAGAATT CCAGTGATAT AGCTAATCAG 
AAGGATGAGG CAAATCAACT GAAAGCTGTC TTTGAAAACA GAGTGAAAGG CCTTTTGCAA 
GAAAACGAGG AGCTAAAAAG TAAAATAGAG AACCTTGAGG CCAAAATTGC TGCACTTCGC 
CAGGAAATTA AAGACTTAAA TGAAGAGAAT GAGAGAATTA AAGACAAACT TTCAAAGTCT 
GAGAAGGAAA AGCAGCAGTT GGCAATTGCC TTGGAAAATT CTAAGGACCA AGAAAAGGAA 
GCTCTGCAGT TTGCAAGAAA GCTCGAAAAT CAACTTGAAG ACACTAAGAG AAGTCTCAGC 
CGAGTGTCTC ATGAGCACCA GCAATTAACA GAGAGATTAA AGGAAGAAGC TAACCAGAAA 
GAACAGCTAA GGAATCTGAA AAATGAAATG GAAAATGAGC GTTGGCAGTT GGACAAGACA 
ATTGAAAAAT TACAAAAAGA GATTTCAGAA AATGTTGAGT TTTCACGGAT GTCCACAGTG 
GAACTTCAGA AGCAGTTTGA TGAATATAAA GACAAGTATC GCAGGGAAAG TGCTGAAATG 
CAGCGGCAGA TAAAAGACAA AAATCTGGAA GTAGAAAGGG CTTGCCAGAC TATCAAAAAA 
CTGCAAGATG AGGTCCGCCA TTTGGAGGAG AATTTGCAAG ATCACCAGAG AGCCCAGGAT 
GAGGCTCTAA CTAAGAATCA TCTTCTTCAG CAGACCATTA AAGACCTTCA GTATGAACTG 
GATGCTAAAA CTCATGTGAA GGACGATCGG ATTAGGCAGA TCAAATCAAT GGAGGACAAG 
TTATCGCAGC TAGAAATGGA ACTGGATGAA GAGAAGAACA ATTCAGACCT TCTGCTAGAG 
AGAGTAACCA GATGCAGAGA GCAGATTGAG CAAACGAGAA GTGAACTATT TCAAGAAAGA 
GCAGCAAAAC AAGATCTAGA GTGCGATAAA ATATCACTGG AAAGACAGAA CAAAGACCTG 
AAGAGCAGAA TAGTTCATCT TGAAAATTCA CACAGATCAA ATAAAGAAGG GCTTGTCGCA 
CAGATGGAAA CGAGAATAGC AGAGCTGGAA GAACGCCTGG AAAATGAAGA AAGGGAACGG 
GCAAGTCTCC AGCTCAACAA CCGCAGGCTG GAGCGGAAAG TTAAGGAGTT AATGATGCAA 
GTAGATGACG AGCACCTTAC TCTAACGGAT CAGAAAGATC AGTTAAGTTT AAGACTGAAA 
TCAATGAAGA GGCAAGTTGA AGAAGCTGAA GAGGAAATAG ACAGACTGGA GAGCGCTAAA 
AAGAAACTGC AGAGGGATCT AGAAGAGCAA ACTGATATAA ACGACCAACT TCAAACTCAA 
CTTACTGCTG CTAAAAAGGA ACTTAGCAGA CGTAAGAGTT CTCCTAGCCG AATGAATGAT 
CTTGAGGATG ATGATGACGA CCTGAGCACT GACGGGGAGA GTCTTTATGA TGGTCCATCA 
GTATATACGT TTTCTAGGGA TGATGGTATA AGGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cgnl1 ( XM_018092419.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947908.2 Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa55068
Clone ID Related Accession (Same CDS sequence)	XM_018092419.2
Accession Version	XM_018092419.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cingulin-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092419.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCAC GCTTTGATGG TTTAAGATAT ATTCAACAGG ACTGTGGTGT TCTCCACCAA 
AGAAGTGATG AACGGCCTCA AAAGCTTAGA AATTCACACA ATATTAAAGA AGGCACTTAT 
GGTGTAAGTA TTAGGGTCCA GGGAATAGAT GGTCACCCCT ACATTGTTTT AAATAATAAA 
GAAGGACATT TGTCAGAAAA TGACTACTCA TCAGACCGTT GTTCCCAGTC CTTAAAAAGG 
CCTGCAGTTA TATCCAGTGA AAACTCATTT CCTGAAAATC CATTTGAAGC ATCTGTTCGC 
AATACAAATA AAACACAGTA TATAAATTCT GGAAATGGAT TCAAAGGTAG AACTGTAAAA 
CAATCAAACT TGCTAAATTT CCAGAAGCAT CCAGAACTTT TAAAACCATA TGATCCAGAT 
GAACACAGAC GAAATGAAGA CAATTTGCCC AATAATCAAT GCAAAATACC TGCTGTAGAG 
GAACCAAAGA AAAATCATAA ACTTTCTGGT TCCTGTAATA AGGGGATTTC AGCTACATAT 
TCGAGTATCA AATATGTGGA TACACCAAAA TACGAAGACA AGGAAGCATC ACTTAATCAA 
ACAACTAAGC AAGACATCCA AAGCAAAATA TCAGTGGATA ACAAAAGCAT TTTAAGCAGC 
CCAGGTGATA CTTCTACCTC AGAGTCTTTC TCATCAAGTA ACGGTTCACT GTCTAGTAAA 
CACTCAACTG CTTATGTGGA GGCCAAAAAA TCAAGGCCAG ATGTTCTTCA GTTTAGGAGA 
CAGGATTCTG CAGGTGCAGT TGAAGGATCG CGCTCACGTA GATCATCTGG ATCCTCAACT 
GCAACTTCTC AGTCTTTTCA GAAATTTTTG CTCGATGAAC AAGAAGATGC TTTGTATGCA 
GATAATGTGA ACCGTCATGA AAACAGAAGG TATATTCCTT TCGTACCAGG AACAGGTCGT 
GACATTGACA CTGACTGTAT TTTGGGAGTA GATGAGTTGA TTGAAAAGTT TGATAGGAAT 
ATTTCCATGC AGAGGAGAGG TAGGTCTGCC AAACGAAACA GAATTCATCC AGAAGACCGC 
AAAAGGGCAA GAAGTGTTGA CAGTGCATTT CCATTTGGCC TTCAAAGTAA TTCAGAATAT 
TTAAATGAGT TCAGTAAAAG TTTGGGAAAG TCAAATGAAC ATTTACTGAA ACCCTCCAAA 
ATCTGCCCGC AAAAATCACT CACTCAGTAC TACAAAATGT CCTTTAAGAG ACAGAACACA 
ACAGGGAGCC TTGTTTCTCA GCCCTCTCTG CTTGATAATG CCCAGAGCAA AGTTTATGGA 
TCCTTGCAGA ATCAGAAGGA AAAAAACACT AATGCAGATG AACCAGACAG CATATCCTAC 
CTTTCATCCA CTTTGCCAAG CAGAGGTAAA AAGTATGATG ATGTAAAAAT GATTAAATCG 
ACTCTACAGT TAAAGGACTG CATTTCTAGC CCACCACGGG AGGGGATAAG GAGAGCTGCA 
GCTACGATGC AGGGCTCATC TCAAGTTGCT CAGGCGACGC CTGACCTTTT GAAGGGACAA 
CAACAAGTTG CACTACAAAC AAATGAGGAA ACTGCAAAAC AAATACTATT TAACTATCTA 
AAAGAAGGGA GCCCTGATAA TGATGATGCA ACCAAAAGAA AGGTCAACCT GGTGTTTGAA 
AAAATCCAGA CTTTAAAATC AAGAGCTGCT AACACAGTAC AGGATCCATC TGCAGATATC 
AAAGTTTTAC TGGAACAGAA AAAAGAACTG GAAAAGAGAG TTGCTGCTTT GCAAGAAAGA 
CTTGATGAAG AAATTAAGAA TCACAAACGT GACAAAGAAG ACTCAAATAC CAAAGCAAAC 
ATGAAAGACT TCCATGACCA GCTGGAGCAA AGCATGGATG AAAATCTTGT TCTTCGGAAG 
AGATTGGGTG AAAGTGAAAA GGAATTAAGA AAGCATCTTG ATGAATTGTT TGAACTGAAG 
ATGGAACAAG AGCAGTATCA GACGGAGATC AGAGACCTGC AGGAACAGTT GTCAGAAATG 
CACGATGAAT TGGATAATGC CAAGAGCTCT GTTGATGATG GGGAAAGGGA AGCATTTATA 
GAGGAGCTGA TGCAAATGAA ACAAAGTTTT CAAGAGGTCT TGTTGGCTAA AGAAGAGCAG 
GAAGACTTAT TAAGGAAACG GGAGCGTGAA CTTACTGCAC TGAAAGGAGC ACTGAAGGAA 
GAGGTAGCAA CTCATGACCA GGAACTTGAT AAGCTCAAGG AGCAGTATGA TAAGGAGCTG 
AAAAGCATCA GAGACAATCT GACTGCAGCA GTAAAGAATT CCAGTGATAT AGCTAATCAG 
AAGGATGAGG CAAATCAACT GAAAGCTGTC TTTGAAAACA GAGTGAAAGG CCTTTTGCAA 
GAAAACGAGG AGCTAAAAAG TAAAATAGAG AACCTTGAGG CCAAAATTGC TGCACTTCGC 
CAGGAAATTA AAGACTTAAA TGAAGAGAAT GAGAGAATTA AAGACAAACT TTCAAAGTCT 
GAGAAGGAAA AGCAGCAGTT GGCAATTGCC TTGGAAAATT CTAAGGACCA AGAAAAGGAA 
GCTCTGCAGT TTGCAAGAAA GCTCGAAAAT CAACTTGAAG ACACTAAGAG AAGTCTCAGC 
CGAGTGTCTC ATGAGCACCA GCAATTAACA GAGAGATTAA AGGAAGAAGC TAACCAGAAA 
GAACAGCTAA GGAATCTGAA AAATGAAATG GAAAATGAGC GTTGGCAGTT GGACAAGACA 
ATTGAAAAAT TACAAAAAGA GATTTCAGAA AATGTTGAGT TTTCACGGAT GTCCACAGTG 
GAACTTCAGA AGCAGTTTGA TGAATATAAA GACAAGTATC GCAGGGAAAG TGCTGAAATG 
CAGCGGCAGA TAAAAGACAA AAATCTGGAA GTAGAAAGGG CTTGCCAGAC TATCAAAAAA 
CTGCAAGATG AGGTCCGCCA TTTGGAGGAG AATTTGCAAG ATCACCAGAG AGCCCAGGAT 
GAGGCTCTAA CTAAGAATCA TCTTCTTCAG CAGACCATTA AAGACCTTCA GTATGAACTG 
GATGCTAAAA CTCATGTGAA GGACGATCGG ATTAGGCAGA TCAAATCAAT GGAGGACAAG 
TTATCGCAGC TAGAAATGGA ACTGGATGAA GAGAAGAACA ATTCAGACCT TCTGCTAGAG 
AGAGTAACCA GATGCAGAGA GCAGATTGAG CAAACGAGAA GTGAACTATT TCAAGAAAGA 
GCAGCAAAAC AAGATCTAGA GTGCGATAAA ATATCACTGG AAAGACAGAA CAAAGACCTG 
AAGAGCAGAA TAGTTCATCT TGAAAATTCA CACAGATCAA ATAAAGAAGG GCTTGTCGCA 
CAGATGGAAA CGAGAATAGC AGAGCTGGAA GAACGCCTGG AAAATGAAGA AAGGGAACGG 
GCAAGTCTCC AGCTCAACAA CCGCAGGCTG GAGCGGAAAG TTAAGGAGTT AATGATGCAA 
GTAGATGACG AGCACCTTAC TCTAACGGAT CAGAAAGATC AGTTAAGTTT AAGACTGAAA 
TCAATGAAGA GGCAAGTTGA AGAAGCTGAA GAGGAAATAG ACAGACTGGA GAGCGCTAAA 
AAGAAACTGC AGAGGGATCT AGAAGAGCAA ACTGATATAA ACGACCAACT TCAAACTCAA 
CTTACTGCTG CTAAAAAGGA ACTTAGACGT AAGAGTTCTC CTAGCCGAAT GAATGATCTT 
GAGGATGATG ATGACGACCT GAGCACTGAC GGGGAGAGTC TTTATGATGG TCCATCAGTA 
TATACGTTTT CTAGGGATGA TGGTATAAGG GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947908.2
CDS	216..4031
Translation	MDSRFDGLRYIQQDCGVLHQRSDERPQKLRNSHNIKEGTYGVSIRVQGIDGHPYIVLNNKEGHLSENDYSSDRCSQSLKRPAVISSENSFPENPFEASVRNTNKTQYINSGNGFKGRTVKQSNLLNFQKHPELLKPYDPDEHRRNEDNLPNNQCKIPAVEEPKKNHKLSGSCNKGISATYSSIKYVDTPKYEDKEASLNQTTKQDIQSKISVDNKSILSSPGDTSTSESFSSSNGSLSSKHSTAYVEAKKSRPDVLQFRRQDSAGAVEGSRSRRSSGSSTATSQSFQKFLLDEQEDALYADNVNRHENRRYIPFVPGTGRDIDTDCILGVDELIEKFDRNISMQRRGRSAKRNRIHPEDRKRARSVDSAFPFGLQSNSEYLNEFSKSLGKSNEHLLKPSKICPQKSLTQYYKMSFKRQNTTGSLVSQPSLLDNAQSKVYGSLQNQKEKNTNADEPDSISYLSSTLPSRGKKYDDVKMIKSTLQLKDCISSPPREGIRRAAATMQGSSQVAQATPDLLKGQQQVALQTNEETAKQILFNYLKEGSPDNDDATKRKVNLVFEKIQTLKSRAANTVQDPSADIKVLLEQKKELEKRVAALQERLDEEIKNHKRDKEDSNTKANMKDFHDQLEQSMDENLVLRKRLGESEKELRKHLDELFELKMEQEQYQTEIRDLQEQLSEMHDELDNAKSSVDDGEREAFIEELMQMKQSFQEVLLAKEEQEDLLRKRERELTALKGALKEEVATHDQELDKLKEQYDKELKSIRDNLTAAVKNSSDIANQKDEANQLKAVFENRVKGLLQENEELKSKIENLEAKIAALRQEIKDLNEENERIKDKLSKSEKEKQQLAIALENSKDQEKEALQFARKLENQLEDTKRSLSRVSHEHQQLTERLKEEANQKEQLRNLKNEMENERWQLDKTIEKLQKEISENVEFSRMSTVELQKQFDEYKDKYRRESAEMQRQIKDKNLEVERACQTIKKLQDEVRHLEENLQDHQRAQDEALTKNHLLQQTIKDLQYELDAKTHVKDDRIRQIKSMEDKLSQLEMELDEEKNNSDLLLERVTRCREQIEQTRSELFQERAAKQDLECDKISLERQNKDLKSRIVHLENSHRSNKEGLVAQMETRIAELEERLENEERERASLQLNNRRLERKVKELMMQVDDEHLTLTDQKDQLSLRLKSMKRQVEEAEEEIDRLESAKKKLQRDLEEQTDINDQLQTQLTAAKKELRRKSSPSRMNDLEDDDDDLSTDGESLYDGPSVYTFSRDDGIRA

Target ORF information:

RefSeq Version	XM_018092419.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cingulin-like 1 (cgnl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092419.2

ORF Insert Sequence:

ATGGATTCAC GCTTTGATGG TTTAAGATAT ATTCAACAGG ACTGTGGTGT TCTCCACCAA 
AGAAGTGATG AACGGCCTCA AAAGCTTAGA AATTCACACA ATATTAAAGA AGGCACTTAT 
GGTGTAAGTA TTAGGGTCCA GGGAATAGAT GGTCACCCCT ACATTGTTTT AAATAATAAA 
GAAGGACATT TGTCAGAAAA TGACTACTCA TCAGACCGTT GTTCCCAGTC CTTAAAAAGG 
CCTGCAGTTA TATCCAGTGA AAACTCATTT CCTGAAAATC CATTTGAAGC ATCTGTTCGC 
AATACAAATA AAACACAGTA TATAAATTCT GGAAATGGAT TCAAAGGTAG AACTGTAAAA 
CAATCAAACT TGCTAAATTT CCAGAAGCAT CCAGAACTTT TAAAACCATA TGATCCAGAT 
GAACACAGAC GAAATGAAGA CAATTTGCCC AATAATCAAT GCAAAATACC TGCTGTAGAG 
GAACCAAAGA AAAATCATAA ACTTTCTGGT TCCTGTAATA AGGGGATTTC AGCTACATAT 
TCGAGTATCA AATATGTGGA TACACCAAAA TACGAAGACA AGGAAGCATC ACTTAATCAA 
ACAACTAAGC AAGACATCCA AAGCAAAATA TCAGTGGATA ACAAAAGCAT TTTAAGCAGC 
CCAGGTGATA CTTCTACCTC AGAGTCTTTC TCATCAAGTA ACGGTTCACT GTCTAGTAAA 
CACTCAACTG CTTATGTGGA GGCCAAAAAA TCAAGGCCAG ATGTTCTTCA GTTTAGGAGA 
CAGGATTCTG CAGGTGCAGT TGAAGGATCG CGCTCACGTA GATCATCTGG ATCCTCAACT 
GCAACTTCTC AGTCTTTTCA GAAATTTTTG CTCGATGAAC AAGAAGATGC TTTGTATGCA 
GATAATGTGA ACCGTCATGA AAACAGAAGG TATATTCCTT TCGTACCAGG AACAGGTCGT 
GACATTGACA CTGACTGTAT TTTGGGAGTA GATGAGTTGA TTGAAAAGTT TGATAGGAAT 
ATTTCCATGC AGAGGAGAGG TAGGTCTGCC AAACGAAACA GAATTCATCC AGAAGACCGC 
AAAAGGGCAA GAAGTGTTGA CAGTGCATTT CCATTTGGCC TTCAAAGTAA TTCAGAATAT 
TTAAATGAGT TCAGTAAAAG TTTGGGAAAG TCAAATGAAC ATTTACTGAA ACCCTCCAAA 
ATCTGCCCGC AAAAATCACT CACTCAGTAC TACAAAATGT CCTTTAAGAG ACAGAACACA 
ACAGGGAGCC TTGTTTCTCA GCCCTCTCTG CTTGATAATG CCCAGAGCAA AGTTTATGGA 
TCCTTGCAGA ATCAGAAGGA AAAAAACACT AATGCAGATG AACCAGACAG CATATCCTAC 
CTTTCATCCA CTTTGCCAAG CAGAGGTAAA AAGTATGATG ATGTAAAAAT GATTAAATCG 
ACTCTACAGT TAAAGGACTG CATTTCTAGC CCACCACGGG AGGGGATAAG GAGAGCTGCA 
GCTACGATGC AGGGCTCATC TCAAGTTGCT CAGGCGACGC CTGACCTTTT GAAGGGACAA 
CAACAAGTTG CACTACAAAC AAATGAGGAA ACTGCAAAAC AAATACTATT TAACTATCTA 
AAAGAAGGGA GCCCTGATAA TGATGATGCA ACCAAAAGAA AGGTCAACCT GGTGTTTGAA 
AAAATCCAGA CTTTAAAATC AAGAGCTGCT AACACAGTAC AGGATCCATC TGCAGATATC 
AAAGTTTTAC TGGAACAGAA AAAAGAACTG GAAAAGAGAG TTGCTGCTTT GCAAGAAAGA 
CTTGATGAAG AAATTAAGAA TCACAAACGT GACAAAGAAG ACTCAAATAC CAAAGCAAAC 
ATGAAAGACT TCCATGACCA GCTGGAGCAA AGCATGGATG AAAATCTTGT TCTTCGGAAG 
AGATTGGGTG AAAGTGAAAA GGAATTAAGA AAGCATCTTG ATGAATTGTT TGAACTGAAG 
ATGGAACAAG AGCAGTATCA GACGGAGATC AGAGACCTGC AGGAACAGTT GTCAGAAATG 
CACGATGAAT TGGATAATGC CAAGAGCTCT GTTGATGATG GGGAAAGGGA AGCATTTATA 
GAGGAGCTGA TGCAAATGAA ACAAAGTTTT CAAGAGGTCT TGTTGGCTAA AGAAGAGCAG 
GAAGACTTAT TAAGGAAACG GGAGCGTGAA CTTACTGCAC TGAAAGGAGC ACTGAAGGAA 
GAGGTAGCAA CTCATGACCA GGAACTTGAT AAGCTCAAGG AGCAGTATGA TAAGGAGCTG 
AAAAGCATCA GAGACAATCT GACTGCAGCA GTAAAGAATT CCAGTGATAT AGCTAATCAG 
AAGGATGAGG CAAATCAACT GAAAGCTGTC TTTGAAAACA GAGTGAAAGG CCTTTTGCAA 
GAAAACGAGG AGCTAAAAAG TAAAATAGAG AACCTTGAGG CCAAAATTGC TGCACTTCGC 
CAGGAAATTA AAGACTTAAA TGAAGAGAAT GAGAGAATTA AAGACAAACT TTCAAAGTCT 
GAGAAGGAAA AGCAGCAGTT GGCAATTGCC TTGGAAAATT CTAAGGACCA AGAAAAGGAA 
GCTCTGCAGT TTGCAAGAAA GCTCGAAAAT CAACTTGAAG ACACTAAGAG AAGTCTCAGC 
CGAGTGTCTC ATGAGCACCA GCAATTAACA GAGAGATTAA AGGAAGAAGC TAACCAGAAA 
GAACAGCTAA GGAATCTGAA AAATGAAATG GAAAATGAGC GTTGGCAGTT GGACAAGACA 
ATTGAAAAAT TACAAAAAGA GATTTCAGAA AATGTTGAGT TTTCACGGAT GTCCACAGTG 
GAACTTCAGA AGCAGTTTGA TGAATATAAA GACAAGTATC GCAGGGAAAG TGCTGAAATG 
CAGCGGCAGA TAAAAGACAA AAATCTGGAA GTAGAAAGGG CTTGCCAGAC TATCAAAAAA 
CTGCAAGATG AGGTCCGCCA TTTGGAGGAG AATTTGCAAG ATCACCAGAG AGCCCAGGAT 
GAGGCTCTAA CTAAGAATCA TCTTCTTCAG CAGACCATTA AAGACCTTCA GTATGAACTG 
GATGCTAAAA CTCATGTGAA GGACGATCGG ATTAGGCAGA TCAAATCAAT GGAGGACAAG 
TTATCGCAGC TAGAAATGGA ACTGGATGAA GAGAAGAACA ATTCAGACCT TCTGCTAGAG 
AGAGTAACCA GATGCAGAGA GCAGATTGAG CAAACGAGAA GTGAACTATT TCAAGAAAGA 
GCAGCAAAAC AAGATCTAGA GTGCGATAAA ATATCACTGG AAAGACAGAA CAAAGACCTG 
AAGAGCAGAA TAGTTCATCT TGAAAATTCA CACAGATCAA ATAAAGAAGG GCTTGTCGCA 
CAGATGGAAA CGAGAATAGC AGAGCTGGAA GAACGCCTGG AAAATGAAGA AAGGGAACGG 
GCAAGTCTCC AGCTCAACAA CCGCAGGCTG GAGCGGAAAG TTAAGGAGTT AATGATGCAA 
GTAGATGACG AGCACCTTAC TCTAACGGAT CAGAAAGATC AGTTAAGTTT AAGACTGAAA 
TCAATGAAGA GGCAAGTTGA AGAAGCTGAA GAGGAAATAG ACAGACTGGA GAGCGCTAAA 
AAGAAACTGC AGAGGGATCT AGAAGAGCAA ACTGATATAA ACGACCAACT TCAAACTCAA 
CTTACTGCTG CTAAAAAGGA ACTTAGACGT AAGAGTTCTC CTAGCCGAAT GAATGATCTT 
GAGGATGATG ATGACGACCT GAGCACTGAC GGGGAGAGTC TTTATGATGG TCCATCAGTA 
TATACGTTTT CTAGGGATGA TGGTATAAGG GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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cgnl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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