mastl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mastl
Entrez Gene ID	100145700
Full Name	microtubule associated serine/threonine kinase like
Synonyms	GW,GWL,MAST-L,greatwall,thc2
General protein information	Preferred Names microtubule associated serine/threonine kinase like Names serine/threonine-protein kinase greatwall Microtubule-associated serine/threonine-protein kinase-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012964312.2	XP_012819766.1	serine/threonine-protein kinase greatwall isoform X1
XM_012964312.3	XP_012819766.1	serine/threonine-protein kinase greatwall isoform X1
XM_031903230.1	XP_031759090.1	serine/threonine-protein kinase greatwall isoform X2
NM_001127074.1	NP_001120546.1	serine/threonine-protein kinase greatwall

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mastl	NP_080255.3
Rattus norvegicus (Norway rat)	Mastl	NP_001100839.1
Gallus gallus (chicken)	MASTL	XP_418589.2
Homo sapiens (human)	MASTL	NP_001165774.1
Pan troglodytes (chimpanzee)	MASTL	XP_001160397.3
	MASTL	XP_001104617.1
Canis lupus familiaris (dog)	MASTL	XP_848866.1
Bos taurus (cattle)	MASTL	NP_001107237.1
Danio rerio (zebrafish)	mastl	NP_001003609.2
Xenopus tropicalis (tropical clawed frog)	mastl	NP_001120546.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mastl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mastl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa25833	NM_001127074.1 Latest version!	Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa31994	XM_012964312.3 Latest version!	Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa59521	XM_031903230.1 Latest version!	Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa31994	XM_012964312.2	Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mastl ( NM_001127074.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001120546.1 Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), mRNA.

Price & Availability↑

CloneID	OXa25833
Clone ID Related Accession (Same CDS sequence)	NM_001127074.1
Accession Version	NM_001127074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase greatwall
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161476.1. ##Evidence-Data-START## Transcript exon combination :: BC161476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGTGG TCGTGGCAGA GACTAGTCAG AATGGAGACA TTTCCCTGTT GTCTGAGAAG 
AAGTTCACGG TACCGCAGCC TCCTTCCATC GAGGAGTTCA GCATTGTGAA ACCTATCAGC 
CGCGGCGCAT TCGGGAAGGT GTATCTGGCT CGCAGGAAAA ACAACAACAA GCTCTTCGCT 
GTCAAGGTTG TCAAAAAGGC TGATATGATA AACAAGAACA TGGTTCAACA GGTCCAGGCG 
GAGAGGGACG CACTTGCTCT GAGCAAAAGT CCTTTTATTG TCCATTTATA CTATTCACTT 
CAGTCTGCCA ACAACATATA CCTGATTATG GAGTATCTCA TTGGTGGGGA TGTGAAGTCT 
CTTCTCCACA TTTATGGATA CTTTGATGAA GAGATGGCAG TTAAATATAT TTCTGAAGTT 
GCACTGGCTC TTGATTACCT GCACAGACAT GGAATAATTC ACAGAGACCT TAAGCCAGAT 
AACATGTTGA TCTCCAATGA GGGGCACATT AAACTGACAG ACTTTGGCCT TTCTAAAGTG 
ACCCTTAAGC GAGAACTTAG TATGATGGAT ATCTTAACTA CACCATCTAT GGCCAAGCCT 
AAGCGCGACT ATTCACGCAC CCCAGGACAA GTGCTTTCTC TTATAAGCTC TTTGGGTTTT 
AACACACCAG TTGGTGGAAG AACACAAGGA TCTATCGCTC AGCAGACTGA AGGAATGCGT 
GGAAATGCAT CAACTCCCCT TCTGATGAAA AAGAAAGAAA ATTCAGTGAA AGGCAATAAG 
CTGATGATAT CCTGCCCCGA AGCTGGTTTG TCCAGTCCTA GCATGCCGGT GAAATGTTTG 
ACACCCAACC TTCTCAAGTG CAGAACCCCC TTCACTACAT CCAGCACAAG CAGCCAGTCG 
CGTATCTGTC TGTCCAGCTT GGAGTCCGAG TGCGGAATGA GTCCTAGATG GGAAAACTGC 
AGCCAGGATG CAGAGGCACC TCCATACTTG AACTCCAGCC GGGTAAAGGA CTGCTCGTCA 
GAACAGGCAA GGAGCAAGAA ACCTATGGGC AGCTCAGCCT CCCAGAATCT GAAGCATTTG 
GAATTTGCCT TCTCCCCAAT TGTTGACCGT AGAACTGGGA AGAAAGCAGG CTTTCAGGAT 
GAGACTGGGG AACTGAGTGA CACTCCGCTT GCCACTCTTG GTGCCAAAGG TGTCATTAGG 
AAATGCCTCT ATGACAATAA TGCACAGGAG AAACATAAAG ATTTAGGCAA GGATGATCAG 
GGAGAACTGG AAAAACTGAC CATCTCTCCT GATTCTCCCC CTTGGTTAGC TAATGGCTCT 
GTAGCACCCA TACAATTTAA TGATGATGAG ATCATTGAAA AGATGGGGAT TAAACGGAAT 
TACGATTTGG TGGAAAAGAG CCCTGAACAG GAAGTTCTTC AGGATAAAAA GACTAATACA 
GACTATAAAC GTGGCTGTAC AATAACAGGT TATCCAGTTT CTCAGAGCAC AGGCCTAACC 
ATGGAAATTA ATTCGCTCTT CCTTTCTGAA CTCAGAAGCA GCACCAATAA TTACGCTAGT 
GACAGAAAGT CTGAGGATGA CTACATATCT GCACCTCGTA CCCATGAAAA CTTAGGATCT 
GGGAACACAA TAGCCAAGAA TTTGCTTTGT GAGCTTGATG ATAATTGTGA ACGGGATGGT 
GAGGCTAATT CGAACTCTGG GTGCGAGGAG GGGGAGAATC AGAAAGAGAG TCTGAATCAG 
GATTCGGAAT CTTCTTCCGC TGACATGTCC GTAACTGAAA ACCAGATAGA AAGGGAGCTT 
TGCCAAGTGG ATAAGAGCAT AAAAGAACTT TCCTTTGAGG AGTCGCCAAG TGAAAGCAAT 
GAGGAGACCA CACCTGAGAA CAAAGGCATG GCATTCATGG CTGAAAATGA TGCGCTGAAA 
CGCGAACCAA ATAGAAGCGT TTTGCCTGAA ACCTTACATA ATGTATTGGC TTCTCCTGCT 
CCAACTAGTG CAATGGCGCA CCCTAGGAGA AAGCCCATGG TAGCTTTCAG AAGTTACAAC 
AGCCCCATCA ATGGCTCCAA CCTGTCAGAA CCTTCCAGAA TTAGTATGAA CTCTGCAGAC 
AAAATCCACT TTTCTTTGGG CTGCACTGGC TCCTTCCCCA TGGCTGTTAC TCCAGCTCAA 
AAGAAAGTCC AGGGTCTTAC AGAGACCCCT TATAGGACCC CGAAGACTGT AAGGAGAGGG 
GGATTACAGG CTGAAAATGA GAGGATCCTT GGAACCCCAG ATTACTTGGC ACCTGAGCTG 
CTTCTTGGAA AGTCTCATGG TCCTGCAGTC GACTGGTGGG CTCTCGGGGT TTGCCTTTTT 
GAGTTCCTAA CTGGTATTCC CCCGTTTAAC GATGAAACTC CATCCCAAGT CTTCCAGAAT 
ATATTAAACA GAGACATTCC TTGGCCTGAA GAAGAAGAGA CCCTTTCAGT CAATGCTCAG 
AGTGCAATAG AAATTCTCCT GGCCATCGAC CAAACCAAGA GAGCTGGGCT GAAAGATTTA 
AAGGCCCACC ATCTCTTCCA CGCCATTGAA TGGGACGACT TGCAGAACCT GCCTATGCCC 
TTCATTCCAC AGCCCGACGA TGAGACAGAC ACAACCTACT TTGAAGCCAG AAACAACGCT 
CAGCACCTTA AGGTCTCTGG GTTCAGTCTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120546.1
CDS	85..2757
Translation	MGVVVAETSQNGDISLLSEKKFTVPQPPSIEEFSIVKPISRGAFGKVYLARRKNNNKLFAVKVVKKADMINKNMVQQVQAERDALALSKSPFIVHLYYSLQSANNIYLIMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLKRELSMMDILTTPSMAKPKRDYSRTPGQVLSLISSLGFNTPVGGRTQGSIAQQTEGMRGNASTPLLMKKKENSVKGNKLMISCPEAGLSSPSMPVKCLTPNLLKCRTPFTTSSTSSQSRICLSSLESECGMSPRWENCSQDAEAPPYLNSSRVKDCSSEQARSKKPMGSSASQNLKHLEFAFSPIVDRRTGKKAGFQDETGELSDTPLATLGAKGVIRKCLYDNNAQEKHKDLGKDDQGELEKLTISPDSPPWLANGSVAPIQFNDDEIIEKMGIKRNYDLVEKSPEQEVLQDKKTNTDYKRGCTITGYPVSQSTGLTMEINSLFLSELRSSTNNYASDRKSEDDYISAPRTHENLGSGNTIAKNLLCELDDNCERDGEANSNSGCEEGENQKESLNQDSESSSADMSVTENQIERELCQVDKSIKELSFEESPSESNEETTPENKGMAFMAENDALKREPNRSVLPETLHNVLASPAPTSAMAHPRRKPMVAFRSYNSPINGSNLSEPSRISMNSADKIHFSLGCTGSFPMAVTPAQKKVQGLTETPYRTPKTVRRGGLQAENERILGTPDYLAPELLLGKSHGPAVDWWALGVCLFEFLTGIPPFNDETPSQVFQNILNRDIPWPEEEETLSVNAQSAIEILLAIDQTKRAGLKDLKAHHLFHAIEWDDLQNLPMPFIPQPDDETDTTYFEARNNAQHLKVSGFSL

Target ORF information:

RefSeq Version	NM_001127074.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127074.1

ORF Insert Sequence:

ATGGGGGTGG TCGTGGCAGA GACTAGTCAG AATGGAGACA TTTCCCTGTT GTCTGAGAAG 
AAGTTCACGG TACCGCAGCC TCCTTCCATC GAGGAGTTCA GCATTGTGAA ACCTATCAGC 
CGCGGCGCAT TCGGGAAGGT GTATCTGGCT CGCAGGAAAA ACAACAACAA GCTCTTCGCT 
GTCAAGGTTG TCAAAAAGGC TGATATGATA AACAAGAACA TGGTTCAACA GGTCCAGGCG 
GAGAGGGACG CACTTGCTCT GAGCAAAAGT CCTTTTATTG TCCATTTATA CTATTCACTT 
CAGTCTGCCA ACAACATATA CCTGATTATG GAGTATCTCA TTGGTGGGGA TGTGAAGTCT 
CTTCTCCACA TTTATGGATA CTTTGATGAA GAGATGGCAG TTAAATATAT TTCTGAAGTT 
GCACTGGCTC TTGATTACCT GCACAGACAT GGAATAATTC ACAGAGACCT TAAGCCAGAT 
AACATGTTGA TCTCCAATGA GGGGCACATT AAACTGACAG ACTTTGGCCT TTCTAAAGTG 
ACCCTTAAGC GAGAACTTAG TATGATGGAT ATCTTAACTA CACCATCTAT GGCCAAGCCT 
AAGCGCGACT ATTCACGCAC CCCAGGACAA GTGCTTTCTC TTATAAGCTC TTTGGGTTTT 
AACACACCAG TTGGTGGAAG AACACAAGGA TCTATCGCTC AGCAGACTGA AGGAATGCGT 
GGAAATGCAT CAACTCCCCT TCTGATGAAA AAGAAAGAAA ATTCAGTGAA AGGCAATAAG 
CTGATGATAT CCTGCCCCGA AGCTGGTTTG TCCAGTCCTA GCATGCCGGT GAAATGTTTG 
ACACCCAACC TTCTCAAGTG CAGAACCCCC TTCACTACAT CCAGCACAAG CAGCCAGTCG 
CGTATCTGTC TGTCCAGCTT GGAGTCCGAG TGCGGAATGA GTCCTAGATG GGAAAACTGC 
AGCCAGGATG CAGAGGCACC TCCATACTTG AACTCCAGCC GGGTAAAGGA CTGCTCGTCA 
GAACAGGCAA GGAGCAAGAA ACCTATGGGC AGCTCAGCCT CCCAGAATCT GAAGCATTTG 
GAATTTGCCT TCTCCCCAAT TGTTGACCGT AGAACTGGGA AGAAAGCAGG CTTTCAGGAT 
GAGACTGGGG AACTGAGTGA CACTCCGCTT GCCACTCTTG GTGCCAAAGG TGTCATTAGG 
AAATGCCTCT ATGACAATAA TGCACAGGAG AAACATAAAG ATTTAGGCAA GGATGATCAG 
GGAGAACTGG AAAAACTGAC CATCTCTCCT GATTCTCCCC CTTGGTTAGC TAATGGCTCT 
GTAGCACCCA TACAATTTAA TGATGATGAG ATCATTGAAA AGATGGGGAT TAAACGGAAT 
TACGATTTGG TGGAAAAGAG CCCTGAACAG GAAGTTCTTC AGGATAAAAA GACTAATACA 
GACTATAAAC GTGGCTGTAC AATAACAGGT TATCCAGTTT CTCAGAGCAC AGGCCTAACC 
ATGGAAATTA ATTCGCTCTT CCTTTCTGAA CTCAGAAGCA GCACCAATAA TTACGCTAGT 
GACAGAAAGT CTGAGGATGA CTACATATCT GCACCTCGTA CCCATGAAAA CTTAGGATCT 
GGGAACACAA TAGCCAAGAA TTTGCTTTGT GAGCTTGATG ATAATTGTGA ACGGGATGGT 
GAGGCTAATT CGAACTCTGG GTGCGAGGAG GGGGAGAATC AGAAAGAGAG TCTGAATCAG 
GATTCGGAAT CTTCTTCCGC TGACATGTCC GTAACTGAAA ACCAGATAGA AAGGGAGCTT 
TGCCAAGTGG ATAAGAGCAT AAAAGAACTT TCCTTTGAGG AGTCGCCAAG TGAAAGCAAT 
GAGGAGACCA CACCTGAGAA CAAAGGCATG GCATTCATGG CTGAAAATGA TGCGCTGAAA 
CGCGAACCAA ATAGAAGCGT TTTGCCTGAA ACCTTACATA ATGTATTGGC TTCTCCTGCT 
CCAACTAGTG CAATGGCGCA CCCTAGGAGA AAGCCCATGG TAGCTTTCAG AAGTTACAAC 
AGCCCCATCA ATGGCTCCAA CCTGTCAGAA CCTTCCAGAA TTAGTATGAA CTCTGCAGAC 
AAAATCCACT TTTCTTTGGG CTGCACTGGC TCCTTCCCCA TGGCTGTTAC TCCAGCTCAA 
AAGAAAGTCC AGGGTCTTAC AGAGACCCCT TATAGGACCC CGAAGACTGT AAGGAGAGGG 
GGATTACAGG CTGAAAATGA GAGGATCCTT GGAACCCCAG ATTACTTGGC ACCTGAGCTG 
CTTCTTGGAA AGTCTCATGG TCCTGCAGTC GACTGGTGGG CTCTCGGGGT TTGCCTTTTT 
GAGTTCCTAA CTGGTATTCC CCCGTTTAAC GATGAAACTC CATCCCAAGT CTTCCAGAAT 
ATATTAAACA GAGACATTCC TTGGCCTGAA GAAGAAGAGA CCCTTTCAGT CAATGCTCAG 
AGTGCAATAG AAATTCTCCT GGCCATCGAC CAAACCAAGA GAGCTGGGCT GAAAGATTTA 
AAGGCCCACC ATCTCTTCCA CGCCATTGAA TGGGACGACT TGCAGAACCT GCCTATGCCC 
TTCATTCCAC AGCCCGACGA TGAGACAGAC ACAACCTACT TTGAAGCCAG AAACAACGCT 
CAGCACCTTA AGGTCTCTGG GTTCAGTCTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mastl ( XM_012964312.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819766.1 Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31994
Clone ID Related Accession (Same CDS sequence)	XM_012964312.2 , XM_012964312.3
Accession Version	XM_012964312.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase greatwall isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAAG GGAAGAAACC AGACCATCAT GTTCTTTGTA TATTGGTTGT CAAAAAGGCT 
GATATGATAA ACAAGAACAT GGTTCAACAG GTCCAGGCGG AGAGGGACGC ACTTGCTCTG 
AGCAAAAGTC CTTTTATTGT CCATTTATAC TATTCACTTC AGTCTGCCAA CAACATATAC 
CTGATTATGG AGTATCTCAT TGGTGGGGAT GTGAAGTCTC TTCTCCACAT TTATGGATAC 
TTTGATGAAG AGATGGCAGT TAAATATATT TCTGAAGTTG CACTGGCTCT TGATTACCTG 
CACAGACATG GAATAATTCA CAGAGACCTT AAGCCAGATA ACATGTTGAT CTCCAATGAG 
GGGCACATTA AACTGACAGA CTTTGGCCTT TCTAAAGTGA CCCTTAAGCG AGAACTTAGT 
ATGATGGATA TCTTAACTAC ACCATCTATG GCCAAGCCTA AGCGCGACTA TTCACGCACC 
CCAGGACAAG TGCTTTCTCT TATAAGCTCT TTGGGTTTTA ACACACCAGT TGGTGGAAGA 
ACACAAGGAT CTATCGCTCA GCAGACTGAA GGAATGCGTG GAAATGCATC AACTCCCCTT 
CTGATGAAAA AGAAAGAAAA TTCAGTGAAA GGCAATAAGC TGATGATATC CTGCCCCGAA 
GCTGGTTTGT CCAGTCCTAG CATGCCGGTG AAATGTTTGA CACCCAACCT TCTCAAGTGC 
AGAACCCCCT TCACTACATC CAGCACAAGC AGCCAGTCGC GTATCTGTCT GTCCAGCTTG 
GAGTCCGAGT GCGGAATGAG TCCTAGATGG GAAAACTGCA GCCAGGATGC AGAGGCACCT 
CCATACTTGA ACTCCAGCCG GGTAAAGGAC TGCTCGTCAG AACAGGCAAG GAGCAAGAAA 
CCTATGGGCA GCTCAGCCTC CCAGAATCTG AAGCATTTGG AATTTGCCTT CTCCCCAATT 
GTTGACCGTA GAACTGGGAA GAAAGCAGGC TTTCAGGATG AGACTGGGGA ACTGAGTGAC 
ACTCCGCTTG CCACTCTTGG TGTCAAAGGT GTCATTAGGA AATGCCTCTA TGACAATAAT 
GCACAGGAGA AACATAAAGA TTTAGGCAAG GATGATCAGG GAGAACTGGA AAAACTGACC 
ATCTCTCCTG ATTCTCCCCC TTGGTTAGCT AATGGCTCTG TAGCACCCAT ACAATTTAAT 
GATGATGAGA TCATTGAAAA GATGGGGATT AAACGGAATT ACGATTTGGT GGAAAAGAGC 
CCTGAACAGG AAGTTCTTCA GGATAAAAAG ACTAATACAG ACTATAAACG TGGCTGTACA 
ATAACAGGTT ATCCAGTTTC TCAGAGCACA GGCCTAACCA TGGAAATTAA TTCGCTCTTC 
CTTTCTGAAC TCAGAAGCAG CACCAATAAT TACGCTAGTG ACAGAAAGTC TGAGGATGAC 
TACATATCTG CACCTCGTAC CCATGAAAAG TTAGGATCTG GGAACACAAT AGCCAAGAAT 
TTGCTTTGTG AGCTTGATGA TAATTGTGAA CGGGATGGTG AGGCTAATTC GAACTCTGGG 
TGCGAGGAGG GGGAGAATCA GAAAGAGAGT CTGAATCAGG ATTCGGAATC TTCTTCCGCT 
GACATGTCCG TAACTGAAAA CCAGATAGAA AGGGAGCTTT GCCAAGTGGA TAAGAGCATA 
AAAGAACTTT CCTTTGAGGA GTCGCCAAGT GAAAGCAATG AGGAGACCAC ACCTGAGAAC 
AAAGGCATGG CATTCATGGC TGAAAATGAT GCGCTGAAAC GCGAACCAAA TAGAAGCGTT 
TTGCCTGAAT CCTTACATAA TGTATTGGCT TCTCCTGCTC CAACTAGTGC AATGGCGCAC 
CCTAGGAGAA AGCCCATGGT AGCTTTCAGA AGTTACAACA GCCCCATCAA TGGCTCCAAC 
CTGTCAGAAC CTTCCAGAAT TAGTATGAAC TCTGCAGACA AAATCCACTT TTCTTTGGGC 
TGCACTGGCT CCTTCCCCAT GGCTGTTACT CCAGCTCAAA AGAAAGTCCA GGGTCTTACA 
GAGACCCCTT ATAGGACCCC GAAGACTGTA AGGAGAGGGG GATTACAGGC TGAAAATGAG 
AGGATCCTTG GAACCCCAGA TTACTTGGCA CCTGAGCTGC TTCTTGGAAA GTCTCATGGT 
CCTGCAGTCG ACTGGTGGGC TCTCGGGGTT TGCCTTTTTG AGTTCCTAAC TGGTATTCCC 
CCGTTTAACG ATGAAACTCC ATCCCAAGTC TTCCAGAATA TATTAAACAG AGACATTCCT 
TGGCCTGAAG AAGAAGAGAC CCTTTCAGTC AATGCTCAGA GTGCAATAGA AATTCTCCTG 
GCCATCGACC AAACCAAGAG AGCTGGGCTG AAAGATTTAA AGGCCCACCA TCTCTTCCAC 
GCCATTGAAT GGGACGACTT GCAGAACCTG CCTATGCCCT TCATTCCACA GCCCGACGAT 
GAGACAGACA CAACCTACTT TGAAGCCAGA AACAACGCTC AGCACCTTAA GGTCTCTGGG 
TTCAGTCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819766.1
CDS	3034..5565
Translation	MAKGKKPDHHVLCILVVKKADMINKNMVQQVQAERDALALSKSPFIVHLYYSLQSANNIYLIMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLKRELSMMDILTTPSMAKPKRDYSRTPGQVLSLISSLGFNTPVGGRTQGSIAQQTEGMRGNASTPLLMKKKENSVKGNKLMISCPEAGLSSPSMPVKCLTPNLLKCRTPFTTSSTSSQSRICLSSLESECGMSPRWENCSQDAEAPPYLNSSRVKDCSSEQARSKKPMGSSASQNLKHLEFAFSPIVDRRTGKKAGFQDETGELSDTPLATLGVKGVIRKCLYDNNAQEKHKDLGKDDQGELEKLTISPDSPPWLANGSVAPIQFNDDEIIEKMGIKRNYDLVEKSPEQEVLQDKKTNTDYKRGCTITGYPVSQSTGLTMEINSLFLSELRSSTNNYASDRKSEDDYISAPRTHEKLGSGNTIAKNLLCELDDNCERDGEANSNSGCEEGENQKESLNQDSESSSADMSVTENQIERELCQVDKSIKELSFEESPSESNEETTPENKGMAFMAENDALKREPNRSVLPESLHNVLASPAPTSAMAHPRRKPMVAFRSYNSPINGSNLSEPSRISMNSADKIHFSLGCTGSFPMAVTPAQKKVQGLTETPYRTPKTVRRGGLQAENERILGTPDYLAPELLLGKSHGPAVDWWALGVCLFEFLTGIPPFNDETPSQVFQNILNRDIPWPEEEETLSVNAQSAIEILLAIDQTKRAGLKDLKAHHLFHAIEWDDLQNLPMPFIPQPDDETDTTYFEARNNAQHLKVSGFSL

Target ORF information:

RefSeq Version	XM_012964312.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964312.2

ORF Insert Sequence:

ATGGCTAAAG GGAAGAAACC AGACCATCAT GTTCTTTGTA TATTGGTTGT CAAAAAGGCT 
GATATGATAA ACAAGAACAT GGTTCAACAG GTCCAGGCGG AGAGGGACGC ACTTGCTCTG 
AGCAAAAGTC CTTTTATTGT CCATTTATAC TATTCACTTC AGTCTGCCAA CAACATATAC 
CTGATTATGG AGTATCTCAT TGGTGGGGAT GTGAAGTCTC TTCTCCACAT TTATGGATAC 
TTTGATGAAG AGATGGCAGT TAAATATATT TCTGAAGTTG CACTGGCTCT TGATTACCTG 
CACAGACATG GAATAATTCA CAGAGACCTT AAGCCAGATA ACATGTTGAT CTCCAATGAG 
GGGCACATTA AACTGACAGA CTTTGGCCTT TCTAAAGTGA CCCTTAAGCG AGAACTTAGT 
ATGATGGATA TCTTAACTAC ACCATCTATG GCCAAGCCTA AGCGCGACTA TTCACGCACC 
CCAGGACAAG TGCTTTCTCT TATAAGCTCT TTGGGTTTTA ACACACCAGT TGGTGGAAGA 
ACACAAGGAT CTATCGCTCA GCAGACTGAA GGAATGCGTG GAAATGCATC AACTCCCCTT 
CTGATGAAAA AGAAAGAAAA TTCAGTGAAA GGCAATAAGC TGATGATATC CTGCCCCGAA 
GCTGGTTTGT CCAGTCCTAG CATGCCGGTG AAATGTTTGA CACCCAACCT TCTCAAGTGC 
AGAACCCCCT TCACTACATC CAGCACAAGC AGCCAGTCGC GTATCTGTCT GTCCAGCTTG 
GAGTCCGAGT GCGGAATGAG TCCTAGATGG GAAAACTGCA GCCAGGATGC AGAGGCACCT 
CCATACTTGA ACTCCAGCCG GGTAAAGGAC TGCTCGTCAG AACAGGCAAG GAGCAAGAAA 
CCTATGGGCA GCTCAGCCTC CCAGAATCTG AAGCATTTGG AATTTGCCTT CTCCCCAATT 
GTTGACCGTA GAACTGGGAA GAAAGCAGGC TTTCAGGATG AGACTGGGGA ACTGAGTGAC 
ACTCCGCTTG CCACTCTTGG TGTCAAAGGT GTCATTAGGA AATGCCTCTA TGACAATAAT 
GCACAGGAGA AACATAAAGA TTTAGGCAAG GATGATCAGG GAGAACTGGA AAAACTGACC 
ATCTCTCCTG ATTCTCCCCC TTGGTTAGCT AATGGCTCTG TAGCACCCAT ACAATTTAAT 
GATGATGAGA TCATTGAAAA GATGGGGATT AAACGGAATT ACGATTTGGT GGAAAAGAGC 
CCTGAACAGG AAGTTCTTCA GGATAAAAAG ACTAATACAG ACTATAAACG TGGCTGTACA 
ATAACAGGTT ATCCAGTTTC TCAGAGCACA GGCCTAACCA TGGAAATTAA TTCGCTCTTC 
CTTTCTGAAC TCAGAAGCAG CACCAATAAT TACGCTAGTG ACAGAAAGTC TGAGGATGAC 
TACATATCTG CACCTCGTAC CCATGAAAAG TTAGGATCTG GGAACACAAT AGCCAAGAAT 
TTGCTTTGTG AGCTTGATGA TAATTGTGAA CGGGATGGTG AGGCTAATTC GAACTCTGGG 
TGCGAGGAGG GGGAGAATCA GAAAGAGAGT CTGAATCAGG ATTCGGAATC TTCTTCCGCT 
GACATGTCCG TAACTGAAAA CCAGATAGAA AGGGAGCTTT GCCAAGTGGA TAAGAGCATA 
AAAGAACTTT CCTTTGAGGA GTCGCCAAGT GAAAGCAATG AGGAGACCAC ACCTGAGAAC 
AAAGGCATGG CATTCATGGC TGAAAATGAT GCGCTGAAAC GCGAACCAAA TAGAAGCGTT 
TTGCCTGAAT CCTTACATAA TGTATTGGCT TCTCCTGCTC CAACTAGTGC AATGGCGCAC 
CCTAGGAGAA AGCCCATGGT AGCTTTCAGA AGTTACAACA GCCCCATCAA TGGCTCCAAC 
CTGTCAGAAC CTTCCAGAAT TAGTATGAAC TCTGCAGACA AAATCCACTT TTCTTTGGGC 
TGCACTGGCT CCTTCCCCAT GGCTGTTACT CCAGCTCAAA AGAAAGTCCA GGGTCTTACA 
GAGACCCCTT ATAGGACCCC GAAGACTGTA AGGAGAGGGG GATTACAGGC TGAAAATGAG 
AGGATCCTTG GAACCCCAGA TTACTTGGCA CCTGAGCTGC TTCTTGGAAA GTCTCATGGT 
CCTGCAGTCG ACTGGTGGGC TCTCGGGGTT TGCCTTTTTG AGTTCCTAAC TGGTATTCCC 
CCGTTTAACG ATGAAACTCC ATCCCAAGTC TTCCAGAATA TATTAAACAG AGACATTCCT 
TGGCCTGAAG AAGAAGAGAC CCTTTCAGTC AATGCTCAGA GTGCAATAGA AATTCTCCTG 
GCCATCGACC AAACCAAGAG AGCTGGGCTG AAAGATTTAA AGGCCCACCA TCTCTTCCAC 
GCCATTGAAT GGGACGACTT GCAGAACCTG CCTATGCCCT TCATTCCACA GCCCGACGAT 
GAGACAGACA CAACCTACTT TGAAGCCAGA AACAACGCTC AGCACCTTAA GGTCTCTGGG 
TTCAGTCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mastl ( XM_012964312.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819766.1 Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31994
Clone ID Related Accession (Same CDS sequence)	XM_012964312.2 , XM_012964312.3
Accession Version	XM_012964312.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase greatwall isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964312.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAAG GGAAGAAACC AGACCATCAT GTTCTTTGTA TATTGGTTGT CAAAAAGGCT 
GATATGATAA ACAAGAACAT GGTTCAACAG GTCCAGGCGG AGAGGGACGC ACTTGCTCTG 
AGCAAAAGTC CTTTTATTGT CCATTTATAC TATTCACTTC AGTCTGCCAA CAACATATAC 
CTGATTATGG AGTATCTCAT TGGTGGGGAT GTGAAGTCTC TTCTCCACAT TTATGGATAC 
TTTGATGAAG AGATGGCAGT TAAATATATT TCTGAAGTTG CACTGGCTCT TGATTACCTG 
CACAGACATG GAATAATTCA CAGAGACCTT AAGCCAGATA ACATGTTGAT CTCCAATGAG 
GGGCACATTA AACTGACAGA CTTTGGCCTT TCTAAAGTGA CCCTTAAGCG AGAACTTAGT 
ATGATGGATA TCTTAACTAC ACCATCTATG GCCAAGCCTA AGCGCGACTA TTCACGCACC 
CCAGGACAAG TGCTTTCTCT TATAAGCTCT TTGGGTTTTA ACACACCAGT TGGTGGAAGA 
ACACAAGGAT CTATCGCTCA GCAGACTGAA GGAATGCGTG GAAATGCATC AACTCCCCTT 
CTGATGAAAA AGAAAGAAAA TTCAGTGAAA GGCAATAAGC TGATGATATC CTGCCCCGAA 
GCTGGTTTGT CCAGTCCTAG CATGCCGGTG AAATGTTTGA CACCCAACCT TCTCAAGTGC 
AGAACCCCCT TCACTACATC CAGCACAAGC AGCCAGTCGC GTATCTGTCT GTCCAGCTTG 
GAGTCCGAGT GCGGAATGAG TCCTAGATGG GAAAACTGCA GCCAGGATGC AGAGGCACCT 
CCATACTTGA ACTCCAGCCG GGTAAAGGAC TGCTCGTCAG AACAGGCAAG GAGCAAGAAA 
CCTATGGGCA GCTCAGCCTC CCAGAATCTG AAGCATTTGG AATTTGCCTT CTCCCCAATT 
GTTGACCGTA GAACTGGGAA GAAAGCAGGC TTTCAGGATG AGACTGGGGA ACTGAGTGAC 
ACTCCGCTTG CCACTCTTGG TGTCAAAGGT GTCATTAGGA AATGCCTCTA TGACAATAAT 
GCACAGGAGA AACATAAAGA TTTAGGCAAG GATGATCAGG GAGAACTGGA AAAACTGACC 
ATCTCTCCTG ATTCTCCCCC TTGGTTAGCT AATGGCTCTG TAGCACCCAT ACAATTTAAT 
GATGATGAGA TCATTGAAAA GATGGGGATT AAACGGAATT ACGATTTGGT GGAAAAGAGC 
CCTGAACAGG AAGTTCTTCA GGATAAAAAG ACTAATACAG ACTATAAACG TGGCTGTACA 
ATAACAGGTT ATCCAGTTTC TCAGAGCACA GGCCTAACCA TGGAAATTAA TTCGCTCTTC 
CTTTCTGAAC TCAGAAGCAG CACCAATAAT TACGCTAGTG ACAGAAAGTC TGAGGATGAC 
TACATATCTG CACCTCGTAC CCATGAAAAG TTAGGATCTG GGAACACAAT AGCCAAGAAT 
TTGCTTTGTG AGCTTGATGA TAATTGTGAA CGGGATGGTG AGGCTAATTC GAACTCTGGG 
TGCGAGGAGG GGGAGAATCA GAAAGAGAGT CTGAATCAGG ATTCGGAATC TTCTTCCGCT 
GACATGTCCG TAACTGAAAA CCAGATAGAA AGGGAGCTTT GCCAAGTGGA TAAGAGCATA 
AAAGAACTTT CCTTTGAGGA GTCGCCAAGT GAAAGCAATG AGGAGACCAC ACCTGAGAAC 
AAAGGCATGG CATTCATGGC TGAAAATGAT GCGCTGAAAC GCGAACCAAA TAGAAGCGTT 
TTGCCTGAAT CCTTACATAA TGTATTGGCT TCTCCTGCTC CAACTAGTGC AATGGCGCAC 
CCTAGGAGAA AGCCCATGGT AGCTTTCAGA AGTTACAACA GCCCCATCAA TGGCTCCAAC 
CTGTCAGAAC CTTCCAGAAT TAGTATGAAC TCTGCAGACA AAATCCACTT TTCTTTGGGC 
TGCACTGGCT CCTTCCCCAT GGCTGTTACT CCAGCTCAAA AGAAAGTCCA GGGTCTTACA 
GAGACCCCTT ATAGGACCCC GAAGACTGTA AGGAGAGGGG GATTACAGGC TGAAAATGAG 
AGGATCCTTG GAACCCCAGA TTACTTGGCA CCTGAGCTGC TTCTTGGAAA GTCTCATGGT 
CCTGCAGTCG ACTGGTGGGC TCTCGGGGTT TGCCTTTTTG AGTTCCTAAC TGGTATTCCC 
CCGTTTAACG ATGAAACTCC ATCCCAAGTC TTCCAGAATA TATTAAACAG AGACATTCCT 
TGGCCTGAAG AAGAAGAGAC CCTTTCAGTC AATGCTCAGA GTGCAATAGA AATTCTCCTG 
GCCATCGACC AAACCAAGAG AGCTGGGCTG AAAGATTTAA AGGCCCACCA TCTCTTCCAC 
GCCATTGAAT GGGACGACTT GCAGAACCTG CCTATGCCCT TCATTCCACA GCCCGACGAT 
GAGACAGACA CAACCTACTT TGAAGCCAGA AACAACGCTC AGCACCTTAA GGTCTCTGGG 
TTCAGTCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819766.1
CDS	2942..5473
Translation	MAKGKKPDHHVLCILVVKKADMINKNMVQQVQAERDALALSKSPFIVHLYYSLQSANNIYLIMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLKRELSMMDILTTPSMAKPKRDYSRTPGQVLSLISSLGFNTPVGGRTQGSIAQQTEGMRGNASTPLLMKKKENSVKGNKLMISCPEAGLSSPSMPVKCLTPNLLKCRTPFTTSSTSSQSRICLSSLESECGMSPRWENCSQDAEAPPYLNSSRVKDCSSEQARSKKPMGSSASQNLKHLEFAFSPIVDRRTGKKAGFQDETGELSDTPLATLGVKGVIRKCLYDNNAQEKHKDLGKDDQGELEKLTISPDSPPWLANGSVAPIQFNDDEIIEKMGIKRNYDLVEKSPEQEVLQDKKTNTDYKRGCTITGYPVSQSTGLTMEINSLFLSELRSSTNNYASDRKSEDDYISAPRTHEKLGSGNTIAKNLLCELDDNCERDGEANSNSGCEEGENQKESLNQDSESSSADMSVTENQIERELCQVDKSIKELSFEESPSESNEETTPENKGMAFMAENDALKREPNRSVLPESLHNVLASPAPTSAMAHPRRKPMVAFRSYNSPINGSNLSEPSRISMNSADKIHFSLGCTGSFPMAVTPAQKKVQGLTETPYRTPKTVRRGGLQAENERILGTPDYLAPELLLGKSHGPAVDWWALGVCLFEFLTGIPPFNDETPSQVFQNILNRDIPWPEEEETLSVNAQSAIEILLAIDQTKRAGLKDLKAHHLFHAIEWDDLQNLPMPFIPQPDDETDTTYFEARNNAQHLKVSGFSL

Target ORF information:

RefSeq Version	XM_012964312.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964312.3

ORF Insert Sequence:

ATGGCTAAAG GGAAGAAACC AGACCATCAT GTTCTTTGTA TATTGGTTGT CAAAAAGGCT 
GATATGATAA ACAAGAACAT GGTTCAACAG GTCCAGGCGG AGAGGGACGC ACTTGCTCTG 
AGCAAAAGTC CTTTTATTGT CCATTTATAC TATTCACTTC AGTCTGCCAA CAACATATAC 
CTGATTATGG AGTATCTCAT TGGTGGGGAT GTGAAGTCTC TTCTCCACAT TTATGGATAC 
TTTGATGAAG AGATGGCAGT TAAATATATT TCTGAAGTTG CACTGGCTCT TGATTACCTG 
CACAGACATG GAATAATTCA CAGAGACCTT AAGCCAGATA ACATGTTGAT CTCCAATGAG 
GGGCACATTA AACTGACAGA CTTTGGCCTT TCTAAAGTGA CCCTTAAGCG AGAACTTAGT 
ATGATGGATA TCTTAACTAC ACCATCTATG GCCAAGCCTA AGCGCGACTA TTCACGCACC 
CCAGGACAAG TGCTTTCTCT TATAAGCTCT TTGGGTTTTA ACACACCAGT TGGTGGAAGA 
ACACAAGGAT CTATCGCTCA GCAGACTGAA GGAATGCGTG GAAATGCATC AACTCCCCTT 
CTGATGAAAA AGAAAGAAAA TTCAGTGAAA GGCAATAAGC TGATGATATC CTGCCCCGAA 
GCTGGTTTGT CCAGTCCTAG CATGCCGGTG AAATGTTTGA CACCCAACCT TCTCAAGTGC 
AGAACCCCCT TCACTACATC CAGCACAAGC AGCCAGTCGC GTATCTGTCT GTCCAGCTTG 
GAGTCCGAGT GCGGAATGAG TCCTAGATGG GAAAACTGCA GCCAGGATGC AGAGGCACCT 
CCATACTTGA ACTCCAGCCG GGTAAAGGAC TGCTCGTCAG AACAGGCAAG GAGCAAGAAA 
CCTATGGGCA GCTCAGCCTC CCAGAATCTG AAGCATTTGG AATTTGCCTT CTCCCCAATT 
GTTGACCGTA GAACTGGGAA GAAAGCAGGC TTTCAGGATG AGACTGGGGA ACTGAGTGAC 
ACTCCGCTTG CCACTCTTGG TGTCAAAGGT GTCATTAGGA AATGCCTCTA TGACAATAAT 
GCACAGGAGA AACATAAAGA TTTAGGCAAG GATGATCAGG GAGAACTGGA AAAACTGACC 
ATCTCTCCTG ATTCTCCCCC TTGGTTAGCT AATGGCTCTG TAGCACCCAT ACAATTTAAT 
GATGATGAGA TCATTGAAAA GATGGGGATT AAACGGAATT ACGATTTGGT GGAAAAGAGC 
CCTGAACAGG AAGTTCTTCA GGATAAAAAG ACTAATACAG ACTATAAACG TGGCTGTACA 
ATAACAGGTT ATCCAGTTTC TCAGAGCACA GGCCTAACCA TGGAAATTAA TTCGCTCTTC 
CTTTCTGAAC TCAGAAGCAG CACCAATAAT TACGCTAGTG ACAGAAAGTC TGAGGATGAC 
TACATATCTG CACCTCGTAC CCATGAAAAG TTAGGATCTG GGAACACAAT AGCCAAGAAT 
TTGCTTTGTG AGCTTGATGA TAATTGTGAA CGGGATGGTG AGGCTAATTC GAACTCTGGG 
TGCGAGGAGG GGGAGAATCA GAAAGAGAGT CTGAATCAGG ATTCGGAATC TTCTTCCGCT 
GACATGTCCG TAACTGAAAA CCAGATAGAA AGGGAGCTTT GCCAAGTGGA TAAGAGCATA 
AAAGAACTTT CCTTTGAGGA GTCGCCAAGT GAAAGCAATG AGGAGACCAC ACCTGAGAAC 
AAAGGCATGG CATTCATGGC TGAAAATGAT GCGCTGAAAC GCGAACCAAA TAGAAGCGTT 
TTGCCTGAAT CCTTACATAA TGTATTGGCT TCTCCTGCTC CAACTAGTGC AATGGCGCAC 
CCTAGGAGAA AGCCCATGGT AGCTTTCAGA AGTTACAACA GCCCCATCAA TGGCTCCAAC 
CTGTCAGAAC CTTCCAGAAT TAGTATGAAC TCTGCAGACA AAATCCACTT TTCTTTGGGC 
TGCACTGGCT CCTTCCCCAT GGCTGTTACT CCAGCTCAAA AGAAAGTCCA GGGTCTTACA 
GAGACCCCTT ATAGGACCCC GAAGACTGTA AGGAGAGGGG GATTACAGGC TGAAAATGAG 
AGGATCCTTG GAACCCCAGA TTACTTGGCA CCTGAGCTGC TTCTTGGAAA GTCTCATGGT 
CCTGCAGTCG ACTGGTGGGC TCTCGGGGTT TGCCTTTTTG AGTTCCTAAC TGGTATTCCC 
CCGTTTAACG ATGAAACTCC ATCCCAAGTC TTCCAGAATA TATTAAACAG AGACATTCCT 
TGGCCTGAAG AAGAAGAGAC CCTTTCAGTC AATGCTCAGA GTGCAATAGA AATTCTCCTG 
GCCATCGACC AAACCAAGAG AGCTGGGCTG AAAGATTTAA AGGCCCACCA TCTCTTCCAC 
GCCATTGAAT GGGACGACTT GCAGAACCTG CCTATGCCCT TCATTCCACA GCCCGACGAT 
GAGACAGACA CAACCTACTT TGAAGCCAGA AACAACGCTC AGCACCTTAA GGTCTCTGGG 
TTCAGTCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mastl ( XM_031903230.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759090.1 Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa59521
Clone ID Related Accession (Same CDS sequence)	XM_031903230.1
Accession Version	XM_031903230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase greatwall isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAACA AGAACATGGT TCAACAGGTC CAGGCGGAGA GGGACGCACT TGCTCTGAGC 
AAAAGTCCTT TTATTGTCCA TTTATACTAT TCACTTCAGT CTGCCAACAA CATATACCTG 
ATTATGGAGT ATCTCATTGG TGGGGATGTG AAGTCTCTTC TCCACATTTA TGGATACTTT 
GATGAAGAGA TGGCAGTTAA ATATATTTCT GAAGTTGCAC TGGCTCTTGA TTACCTGCAC 
AGACATGGAA TAATTCACAG AGACCTTAAG CCAGATAACA TGTTGATCTC CAATGAGGGG 
CACATTAAAC TGACAGACTT TGGCCTTTCT AAAGTGACCC TTAAGCGAGA ACTTAGTATG 
ATGGATATCT TAACTACACC ATCTATGGCC AAGCCTAAGC GCGACTATTC ACGCACCCCA 
GGACAAGTGC TTTCTCTTAT AAGCTCTTTG GGTTTTAACA CACCAGTTGG TGGAAGAACA 
CAAGGATCTA TCGCTCAGCA GACTGAAGGA ATGCGTGGAA ATGCATCAAC TCCCCTTCTG 
ATGAAAAAGA AAGAAAATTC AGTGAAAGGC AATAAGCTGA TGATATCCTG CCCCGAAGCT 
GGTTTGTCCA GTCCTAGCAT GCCGGTGAAA TGTTTGACAC CCAACCTTCT CAAGTGCAGA 
ACCCCCTTCA CTACATCCAG CACAAGCAGC CAGTCGCGTA TCTGTCTGTC CAGCTTGGAG 
TCCGAGTGCG GAATGAGTCC TAGATGGGAA AACTGCAGCC AGGATGCAGA GGCACCTCCA 
TACTTGAACT CCAGCCGGGT AAAGGACTGC TCGTCAGAAC AGGCAAGGAG CAAGAAACCT 
ATGGGCAGCT CAGCCTCCCA GAATCTGAAG CATTTGGAAT TTGCCTTCTC CCCAATTGTT 
GACCGTAGAA CTGGGAAGAA AGCAGGCTTT CAGGATGAGA CTGGGGAACT GAGTGACACT 
CCGCTTGCCA CTCTTGGTGT CAAAGGTGTC ATTAGGAAAT GCCTCTATGA CAATAATGCA 
CAGGAGAAAC ATAAAGATTT AGGCAAGGAT GATCAGGGAG AACTGGAAAA ACTGACCATC 
TCTCCTGATT CTCCCCCTTG GTTAGCTAAT GGCTCTGTAG CACCCATACA ATTTAATGAT 
GATGAGATCA TTGAAAAGAT GGGGATTAAA CGGAATTACG ATTTGGTGGA AAAGAGCCCT 
GAACAGGAAG TTCTTCAGGA TAAAAAGACT AATACAGACT ATAAACGTGG CTGTACAATA 
ACAGGTTATC CAGTTTCTCA GAGCACAGGC CTAACCATGG AAATTAATTC GCTCTTCCTT 
TCTGAACTCA GAAGCAGCAC CAATAATTAC GCTAGTGACA GAAAGTCTGA GGATGACTAC 
ATATCTGCAC CTCGTACCCA TGAAAAGTTA GGATCTGGGA ACACAATAGC CAAGAATTTG 
CTTTGTGAGC TTGATGATAA TTGTGAACGG GATGGTGAGG CTAATTCGAA CTCTGGGTGC 
GAGGAGGGGG AGAATCAGAA AGAGAGTCTG AATCAGGATT CGGAATCTTC TTCCGCTGAC 
ATGTCCGTAA CTGAAAACCA GATAGAAAGG GAGCTTTGCC AAGTGGATAA GAGCATAAAA 
GAACTTTCCT TTGAGGAGTC GCCAAGTGAA AGCAATGAGG AGACCACACC TGAGAACAAA 
GGCATGGCAT TCATGGCTGA AAATGATGCG CTGAAACGCG AACCAAATAG AAGCGTTTTG 
CCTGAATCCT TACATAATGT ATTGGCTTCT CCTGCTCCAA CTAGTGCAAT GGCGCACCCT 
AGGAGAAAGC CCATGGTAGC TTTCAGAAGT TACAACAGCC CCATCAATGG CTCCAACCTG 
TCAGAACCTT CCAGAATTAG TATGAACTCT GCAGACAAAA TCCACTTTTC TTTGGGCTGC 
ACTGGCTCCT TCCCCATGGC TGTTACTCCA GCTCAAAAGA AAGTCCAGGG TCTTACAGAG 
ACCCCTTATA GGACCCCGAA GACTGTAAGG AGAGGGGGAT TACAGGCTGA AAATGAGAGG 
ATCCTTGGAA CCCCAGATTA CTTGGCACCT GAGCTGCTTC TTGGAAAGTC TCATGGTCCT 
GCAGTCGACT GGTGGGCTCT CGGGGTTTGC CTTTTTGAGT TCCTAACTGG TATTCCCCCG 
TTTAACGATG AAACTCCATC CCAAGTCTTC CAGAATATAT TAAACAGAGA CATTCCTTGG 
CCTGAAGAAG AAGAGACCCT TTCAGTCAAT GCTCAGAGTG CAATAGAAAT TCTCCTGGCC 
ATCGACCAAA CCAAGAGAGC TGGGCTGAAA GATTTAAAGG CCCACCATCT CTTCCACGCC 
ATTGAATGGG ACGACTTGCA GAACCTGCCT ATGCCCTTCA TTCCACAGCC CGACGATGAG 
ACAGACACAA CCTACTTTGA AGCCAGAAAC AACGCTCAGC ACCTTAAGGT CTCTGGGTTC 
AGTCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759090.1
CDS	40..2508
Translation	MINKNMVQQVQAERDALALSKSPFIVHLYYSLQSANNIYLIMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLKRELSMMDILTTPSMAKPKRDYSRTPGQVLSLISSLGFNTPVGGRTQGSIAQQTEGMRGNASTPLLMKKKENSVKGNKLMISCPEAGLSSPSMPVKCLTPNLLKCRTPFTTSSTSSQSRICLSSLESECGMSPRWENCSQDAEAPPYLNSSRVKDCSSEQARSKKPMGSSASQNLKHLEFAFSPIVDRRTGKKAGFQDETGELSDTPLATLGVKGVIRKCLYDNNAQEKHKDLGKDDQGELEKLTISPDSPPWLANGSVAPIQFNDDEIIEKMGIKRNYDLVEKSPEQEVLQDKKTNTDYKRGCTITGYPVSQSTGLTMEINSLFLSELRSSTNNYASDRKSEDDYISAPRTHEKLGSGNTIAKNLLCELDDNCERDGEANSNSGCEEGENQKESLNQDSESSSADMSVTENQIERELCQVDKSIKELSFEESPSESNEETTPENKGMAFMAENDALKREPNRSVLPESLHNVLASPAPTSAMAHPRRKPMVAFRSYNSPINGSNLSEPSRISMNSADKIHFSLGCTGSFPMAVTPAQKKVQGLTETPYRTPKTVRRGGLQAENERILGTPDYLAPELLLGKSHGPAVDWWALGVCLFEFLTGIPPFNDETPSQVFQNILNRDIPWPEEEETLSVNAQSAIEILLAIDQTKRAGLKDLKAHHLFHAIEWDDLQNLPMPFIPQPDDETDTTYFEARNNAQHLKVSGFSL

Target ORF information:

RefSeq Version	XM_031903230.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated serine/threonine kinase like (mastl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903230.1

ORF Insert Sequence:

ATGATAAACA AGAACATGGT TCAACAGGTC CAGGCGGAGA GGGACGCACT TGCTCTGAGC 
AAAAGTCCTT TTATTGTCCA TTTATACTAT TCACTTCAGT CTGCCAACAA CATATACCTG 
ATTATGGAGT ATCTCATTGG TGGGGATGTG AAGTCTCTTC TCCACATTTA TGGATACTTT 
GATGAAGAGA TGGCAGTTAA ATATATTTCT GAAGTTGCAC TGGCTCTTGA TTACCTGCAC 
AGACATGGAA TAATTCACAG AGACCTTAAG CCAGATAACA TGTTGATCTC CAATGAGGGG 
CACATTAAAC TGACAGACTT TGGCCTTTCT AAAGTGACCC TTAAGCGAGA ACTTAGTATG 
ATGGATATCT TAACTACACC ATCTATGGCC AAGCCTAAGC GCGACTATTC ACGCACCCCA 
GGACAAGTGC TTTCTCTTAT AAGCTCTTTG GGTTTTAACA CACCAGTTGG TGGAAGAACA 
CAAGGATCTA TCGCTCAGCA GACTGAAGGA ATGCGTGGAA ATGCATCAAC TCCCCTTCTG 
ATGAAAAAGA AAGAAAATTC AGTGAAAGGC AATAAGCTGA TGATATCCTG CCCCGAAGCT 
GGTTTGTCCA GTCCTAGCAT GCCGGTGAAA TGTTTGACAC CCAACCTTCT CAAGTGCAGA 
ACCCCCTTCA CTACATCCAG CACAAGCAGC CAGTCGCGTA TCTGTCTGTC CAGCTTGGAG 
TCCGAGTGCG GAATGAGTCC TAGATGGGAA AACTGCAGCC AGGATGCAGA GGCACCTCCA 
TACTTGAACT CCAGCCGGGT AAAGGACTGC TCGTCAGAAC AGGCAAGGAG CAAGAAACCT 
ATGGGCAGCT CAGCCTCCCA GAATCTGAAG CATTTGGAAT TTGCCTTCTC CCCAATTGTT 
GACCGTAGAA CTGGGAAGAA AGCAGGCTTT CAGGATGAGA CTGGGGAACT GAGTGACACT 
CCGCTTGCCA CTCTTGGTGT CAAAGGTGTC ATTAGGAAAT GCCTCTATGA CAATAATGCA 
CAGGAGAAAC ATAAAGATTT AGGCAAGGAT GATCAGGGAG AACTGGAAAA ACTGACCATC 
TCTCCTGATT CTCCCCCTTG GTTAGCTAAT GGCTCTGTAG CACCCATACA ATTTAATGAT 
GATGAGATCA TTGAAAAGAT GGGGATTAAA CGGAATTACG ATTTGGTGGA AAAGAGCCCT 
GAACAGGAAG TTCTTCAGGA TAAAAAGACT AATACAGACT ATAAACGTGG CTGTACAATA 
ACAGGTTATC CAGTTTCTCA GAGCACAGGC CTAACCATGG AAATTAATTC GCTCTTCCTT 
TCTGAACTCA GAAGCAGCAC CAATAATTAC GCTAGTGACA GAAAGTCTGA GGATGACTAC 
ATATCTGCAC CTCGTACCCA TGAAAAGTTA GGATCTGGGA ACACAATAGC CAAGAATTTG 
CTTTGTGAGC TTGATGATAA TTGTGAACGG GATGGTGAGG CTAATTCGAA CTCTGGGTGC 
GAGGAGGGGG AGAATCAGAA AGAGAGTCTG AATCAGGATT CGGAATCTTC TTCCGCTGAC 
ATGTCCGTAA CTGAAAACCA GATAGAAAGG GAGCTTTGCC AAGTGGATAA GAGCATAAAA 
GAACTTTCCT TTGAGGAGTC GCCAAGTGAA AGCAATGAGG AGACCACACC TGAGAACAAA 
GGCATGGCAT TCATGGCTGA AAATGATGCG CTGAAACGCG AACCAAATAG AAGCGTTTTG 
CCTGAATCCT TACATAATGT ATTGGCTTCT CCTGCTCCAA CTAGTGCAAT GGCGCACCCT 
AGGAGAAAGC CCATGGTAGC TTTCAGAAGT TACAACAGCC CCATCAATGG CTCCAACCTG 
TCAGAACCTT CCAGAATTAG TATGAACTCT GCAGACAAAA TCCACTTTTC TTTGGGCTGC 
ACTGGCTCCT TCCCCATGGC TGTTACTCCA GCTCAAAAGA AAGTCCAGGG TCTTACAGAG 
ACCCCTTATA GGACCCCGAA GACTGTAAGG AGAGGGGGAT TACAGGCTGA AAATGAGAGG 
ATCCTTGGAA CCCCAGATTA CTTGGCACCT GAGCTGCTTC TTGGAAAGTC TCATGGTCCT 
GCAGTCGACT GGTGGGCTCT CGGGGTTTGC CTTTTTGAGT TCCTAACTGG TATTCCCCCG 
TTTAACGATG AAACTCCATC CCAAGTCTTC CAGAATATAT TAAACAGAGA CATTCCTTGG 
CCTGAAGAAG AAGAGACCCT TTCAGTCAAT GCTCAGAGTG CAATAGAAAT TCTCCTGGCC 
ATCGACCAAA CCAAGAGAGC TGGGCTGAAA GATTTAAAGG CCCACCATCT CTTCCACGCC 
ATTGAATGGG ACGACTTGCA GAACCTGCCT ATGCCCTTCA TTCCACAGCC CGACGATGAG 
ACAGACACAA CCTACTTTGA AGCCAGAAAC AACGCTCAGC ACCTTAAGGT CTCTGGGTTC 
AGTCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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mastl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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