gRNAs
The following sirt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sirt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OXa23464 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001142909.1 | |
| Accession Version | NM_001142909.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-21 | |
| Organism | Xenopus tropicalis(tropical clawed frog) | |
| Product | NAD-dependent protein deacetylase sirtuin-1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161420.1. ##Evidence-Data-START## Transcript exon combination :: BC161420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCGGACT CAGATCGTGT AGGTTTTCCC ATTGCCGGCG CTGTGTTAGC TGTCTCAAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001136381.1 |
| CDS | 27..2006 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001142909.1 |
1 | ATGGCGGACT CAGATCGTGT AGGTTTTCCC ATTGCCGGCG CTGTGTTAGC TGTCTCAAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OXa31974 | |
| Clone ID Related Accession (Same CDS sequence) | XM_012966096.2 | |
| Accession Version | XM_012966096.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-09-07 | |
| Organism | Xenopus tropicalis(tropical clawed frog) | |
| Product | NAD-dependent protein deacetylase sirtuin-1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGCGACT CCTTCTCTGG CAGTGGGAAG ATGGCGGACT CAGATCGTGT AGGTTTTCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_012821550.1 |
| CDS | 403..2526 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_012966096.2 |
1 | ATGGGCGACT CCTTCTCTGG CAGTGGGAAG ATGGCGGACT CAGATCGTGT AGGTTTTCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OXa61241 | |
| Clone ID Related Accession (Same CDS sequence) | XM_012966096.3 | |
| Accession Version | XM_012966096.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-17 | |
| Organism | Xenopus tropicalis(tropical clawed frog) | |
| Product | NAD-dependent protein deacetylase sirtuin-1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGACACTTT ATTGCCGATT TCGCTTTCCA ACACCCTCTC CGTTCGTTGT GGCGGTGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_012821550.2 |
| CDS | 7..2304 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_012966096.3 |
1 | ATGACACTTT ATTGCCGATT TCGCTTTCCA ACACCCTCTC CGTTCGTTGT GGCGGTGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative. |
