nsd1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004912865.3	XP_004912922.1	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X2
XM_004912862.4	XP_004912919.2	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_031898843.1	XP_031754703.1	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912864.3	XP_004912921.1	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912858.4	XP_004912915.2	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912861.4	XP_004912918.2	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912864.4	XP_004912921.2	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_018092215.2	XP_017947704.2	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912865.4	XP_004912922.2	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X2
XM_002936951.4	XP_002936997.2	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912861.3	XP_004912918.1	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912862.3	XP_004912919.1	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912860.3	XP_004912917.1	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912860.4	XP_004912917.2	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_002936951.5	XP_002936997.3	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912858.3	XP_004912915.1	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912859.3	XP_004912916.1	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912863.3	XP_004912920.1	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_018092215.1	XP_017947704.1	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
XM_004912863.4	XP_004912920.2	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa54555	XM_004912863.4 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa54555	XM_004912864.4 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa54555	XM_004912858.4 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa54555	XM_018092215.2 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa54555	XM_002936951.5 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa54555	XM_031898843.1 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa54555	XM_004912860.4 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa54555	XM_004912861.4 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa54555	XM_004912862.4 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa54556	XM_004912865.4 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa07643	XM_004912862.3 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa07643	XM_004912863.3 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa07643	XM_004912860.3 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa07643	XM_004912858.3 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa07643	XM_004912861.3 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa07643	XM_004912859.3 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa07643	XM_002936951.4 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa07643	XM_018092215.1 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa07643	XM_004912864.3 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa07644	XM_004912865.3 Latest version!	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

nsd1 ( XM_004912862.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912919.2 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa54555
Clone ID Related Accession (Same CDS sequence)	XM_004912862.4 , XM_031898843.1 , XM_004912858.4 , XM_004912861.4 , XM_004912864.4 , XM_018092215.2 , XM_004912860.4 , XM_002936951.5 , XM_004912863.4
Accession Version	XM_004912862.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912862.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912919.2
CDS	339..7661
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQDPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPVAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIPAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912862.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912862.4

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_031898843.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754703.1 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X9, mRNA.

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CloneID	OXa54555
Clone ID Related Accession (Same CDS sequence)	XM_004912862.4 , XM_031898843.1 , XM_004912858.4 , XM_004912861.4 , XM_004912864.4 , XM_018092215.2 , XM_004912860.4 , XM_002936951.5 , XM_004912863.4
Accession Version	XM_031898843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754703.1
CDS	250..7572
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQDPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPVAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIPAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_031898843.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898843.1

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912858.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912915.2 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X2, mRNA.

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CloneID	OXa54555
Clone ID Related Accession (Same CDS sequence)	XM_004912862.4 , XM_031898843.1 , XM_004912858.4 , XM_004912861.4 , XM_004912864.4 , XM_018092215.2 , XM_004912860.4 , XM_002936951.5 , XM_004912863.4
Accession Version	XM_004912858.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912858.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912915.2
CDS	343..7665
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQDPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPVAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIPAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912858.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912858.4

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912861.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912918.2 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X3, mRNA.

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CloneID	OXa54555
Clone ID Related Accession (Same CDS sequence)	XM_004912862.4 , XM_031898843.1 , XM_004912858.4 , XM_004912861.4 , XM_004912864.4 , XM_018092215.2 , XM_004912860.4 , XM_002936951.5 , XM_004912863.4
Accession Version	XM_004912861.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912861.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912918.2
CDS	125..7447
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQDPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPVAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIPAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912861.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912861.4

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912864.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912921.2 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X6, mRNA.

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CloneID	OXa54555
Clone ID Related Accession (Same CDS sequence)	XM_004912862.4 , XM_031898843.1 , XM_004912858.4 , XM_004912861.4 , XM_004912864.4 , XM_018092215.2 , XM_004912860.4 , XM_002936951.5 , XM_004912863.4
Accession Version	XM_004912864.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912864.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912921.2
CDS	92..7414
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQDPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPVAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIPAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912864.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912864.4

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_018092215.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947704.2 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X8, mRNA.

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CloneID	OXa54555
Clone ID Related Accession (Same CDS sequence)	XM_004912862.4 , XM_031898843.1 , XM_004912858.4 , XM_004912861.4 , XM_004912864.4 , XM_018092215.2 , XM_004912860.4 , XM_002936951.5 , XM_004912863.4
Accession Version	XM_018092215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092215.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947704.2
CDS	315..7637
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQDPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPVAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIPAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_018092215.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092215.2

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912860.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912917.2 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X7, mRNA.

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CloneID	OXa54555
Clone ID Related Accession (Same CDS sequence)	XM_004912862.4 , XM_031898843.1 , XM_004912858.4 , XM_004912861.4 , XM_004912864.4 , XM_018092215.2 , XM_004912860.4 , XM_002936951.5 , XM_004912863.4
Accession Version	XM_004912860.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912860.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912917.2
CDS	95..7417
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQDPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPVAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIPAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912860.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912860.4

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_002936951.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936997.3 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X1, mRNA.

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CloneID	OXa54555
Clone ID Related Accession (Same CDS sequence)	XM_004912862.4 , XM_031898843.1 , XM_004912858.4 , XM_004912861.4 , XM_004912864.4 , XM_018092215.2 , XM_004912860.4 , XM_002936951.5 , XM_004912863.4
Accession Version	XM_002936951.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936951.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936997.3
CDS	301..7623
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQDPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPVAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIPAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_002936951.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936951.5

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912863.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912920.2 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X5, mRNA.

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CloneID	OXa54555
Clone ID Related Accession (Same CDS sequence)	XM_004912862.4 , XM_031898843.1 , XM_004912858.4 , XM_004912861.4 , XM_004912864.4 , XM_018092215.2 , XM_004912860.4 , XM_002936951.5 , XM_004912863.4
Accession Version	XM_004912863.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912863.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912920.2
CDS	90..7412
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQDPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPVAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIPAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912863.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912863.4

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAGTTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCCAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912865.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912922.2 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X10, mRNA.

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CloneID	OXa54556
Clone ID Related Accession (Same CDS sequence)	XM_004912865.4
Accession Version	XM_004912865.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GCTATCACAC CTGCTTTGTT TGTAAGGAAT CTGACCAAGG AGTGAAGCGC 
TGCATGCTGC CCCTCTGTGG CAAATATTAT CATGAAGAAT GTGCCCTTAA ATACCCTCCT 
GCAACTCAAC AAAACAGAGG CTTCCGCTGT TCTCTTCATA TCTGTTCAAC CTGTTATGCC 
ACCAATCCAA GCAACCCCTC TGCATCTAAA GGCCGCTTGA TGAGATGTGT ACGCTGCCCA 
GTTGCATACC ATGCAAATGA TTTCTGCCTG CCAGCAGGAG CAGTTACATT GGCTTCCAAC 
AGTATTATTT GCCCCAACCA CTTCACCCCA AGGCGTGGAT GCAAGAACCA TGAGCATGTA 
AATGTCAGCT GGTGTTTTGT ATGCTCAGAA GGTGGAAGCC TCCTTTGCTG TGAATCCTGT 
CCTGCTGCTT TTCACAGAGA ATGCTTAAAC ATTGATATGC CAGAGGGCAG CTGGTTCTGC 
AATGATTGCA AAGCTGGCAA AAAGCCACAT TACAAAGAAG TGGTGTGGGT GAAGGTGGGG 
AGATACAGGT GGTGGCCAGC AGAGGTTTGT CATCCAAAAA CCATTCCACC CAACATCCAG 
AAGATGAAGC ATGACATTGG CGAGTTCCCA GTTGTCTTCT TTGGTTCCAA AGACTATTTG 
TGGACACACC AGGCCCGAGT GTTCCCATAT ATGGAAGGAG ATGCTCTCAA CAAAGACAAA 
ATGAGCAAGG GAATGGATGC TGTTTACAAA AAGGGGCTGA TGGAAGCTGC AGAAAGGTTT 
GAAGAATTAA AGGCCCAGAA AGAAATGAGA CAGTTACAGG AAGATAAAAA GAATGACAAG 
AAACCTCCAC CATATAAACA CATAAAAGTC AACAGGCCGG TTGGAAAAGT TCAGATTTTA 
ACTGCAGATC TTTCCGAAAT CCCTCGTTGC AACTGCAAGG CCACTGATGA AAATCCATGT 
GGGCAGGATT CCGAGTGCAT CAACCGTATG CTGCTGTACG AGTGCCATCC CTCAGTGTGC 
CCAGCAGGGG AGCGCTGTCA GAATCAAGCC TTCTCCAAAC GCCAGTACCC AGAAGTTGAA 
ATCTTTCGAA CTCTAAGTCG TGGATGGGGG CTGCGGTGTA GGACTGACAT TAAAAAGGGA 
GAATTTGTGA ATGAATATGT GGGTGAAATG ATTGATGAAG AGGAATGCCG TGCTCGCATC 
CGTTACGCCC AAGAGCAAGA CATCACAAAC TTCTACATGC TCACACTAGA TAAGGACCGT 
GTGATTGATG CAGGGCCCAA AGGTAACTTT GCACGTTTTA TGAATCACTG CTGCCAGCCT 
AACTGCGAGA CTCAGAAATG GACTGTGAAT GGTGATACAC GAGTTGGGCT CTTTGCTCTG 
TGTGATATCA AAGCAGGGGT AGAGTTAACA TTTAACTACA ACTTGGAGTG TCTTGGGAAT 
GGAAAGACTG TTTGCAAATG CGGAGCACCA AACTGCAGTG GTTTCCTCGG AGTGCGCCCC 
AAGAATCAGC CAGTCTCATC TGAGGACAAG GGTAAAAAGC GGAAGCAGTA TGTGAAACGT 
AAAAAAAGTG AGGTAGTCAA AGAACATGAA GATGAGTGTT TCAGCTGTGG AGATGGAGGA 
CAGCTTGTAT CTTGCAAGAA ACCAGGTTGC CCTAAAGTAT ACCATGCAGA ATGCTTGAAA 
TTAACCCGTA GACCAGCTGG TAAGTGGGAG TGTCCTTGGC ATCAATGTGA CATCTGTCAC 
AAGGAGGCTG CTTCTCTATG TGAGATGTGT CCAAGCTCCT TCTGCAAGCA GCACCGTGAG 
GGAATGCTCT TCATCTCTAA GCTTGACGGA CGGTTATCCT GCACAGAGCA TGACCCATGT 
GGGCCTCATC CCTTAGAGCC AGGAGAAATT AGGGAGTATG ACTCTTCTAA TGAACAGAAC 
GATTCTGCTG GCCCCTCACT TCCAAAGAAG AAAACACTGG ACAGTGCAAA GGGCAAAACA 
GCATCTTCCA ATTCACACAA GAAAAATGTT TCAGTGACTG AGGTGTTGCA TACACCAACA 
ACCGCTGGTT CTACATCTAG TGGCAATGTT AGCAAGGAAA ATGTGGGGTC TGTTGAGCAA 
AAATCATTGT CTGCTGGAAA AATACTCCTT GCCTCTGCTG GGCAAAAGTG TACTACTGGC 
AGTAAGTTGT TACTTGCCTC AGCAATTCAG AAATCATTGC CTGCTGGGAA GGTAGTCTTG 
ACCTCCTTGG GGAAAAAATC ATTGCCTGCT GGTAAGGTAC TGTTGGCTTC CATTGGAAAG 
AAGCAACTGT CTGCTGGGAA AGTTTTGGTG ACCTCTCTTG GGAAGAATTC TCCATCTGTT 
GGTAGGATGA TTATATCCAC AGCAGGAAAG CATTCTTTAC CAGCAGGAAA AGTTTTGTTA 
GCATCAGCTG GAAAGTATAC AACAGCAGGT AAAGTTCTGT TGACTTCTGC CACCAACCAG 
CATTCAACTG CTGAAAAAAA CAAGGCTGAT AAACAGAATG ATGTTAAAGA AACCCAAAAT 
TCATTGGACC CTGACACTAA ACATCGCATA ACTAAAAAGC CGCTTAATAA ACCATCTAAA 
GATAAGTTAA ATACTACTTT AGAGCAACCA TTGACTCAAA ACCACACAAC TGGGATTCAG 
AAACCTGTAA GCCCATGCCA AGTAGAAAGA CCTTCTAACC CATCCCTCAC AGGGAGGCCT 
GAAAAGCCTA AAGATAAGCA TCAACCAGAC AGTGAAGTTC ATCCTAAGAT CCTTTTAAAG 
AGGTCAACCA TGCCAACCTC CCGAAGGTCC CTGAGCGCAC ATAAAGAGAG GTTTCAAATT 
TCAACCAAAA AAGAAGGGTC TCTTGAAAGC AGCCAGGTAG AGAACCATGC CACTCCAAGA 
AAAGATTCTT GTTCTCCAAC TGTAAAGGAG AAGCCCAGTG CACCAAGTGA GAGATCTTTA 
TCTACAGTAG AGTCCCCTTC TATCCCAGCA GCTCCAGAGA ACTCTTCAGG CAGAGCAATA 
TCATGTACAC TTGCCTCCTC AGAAAAAACA GTTAGCACTT CTGAAATGTC ACTCAGCTCT 
GTTGTACAAG AGAAGACTAG TTCCACTGAA CCAAACTCTT CTTCAATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912922.2
CDS	301..7611
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQDPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPVAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIPAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912865.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912865.4

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGA TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG TGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAGGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCC AGCTGTAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACAGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GCTATCACAC CTGCTTTGTT TGTAAGGAAT CTGACCAAGG AGTGAAGCGC 
TGCATGCTGC CCCTCTGTGG CAAATATTAT CATGAAGAAT GTGCCCTTAA ATACCCTCCT 
GCAACTCAAC AAAACAGAGG CTTCCGCTGT TCTCTTCATA TCTGTTCAAC CTGTTATGCC 
ACCAATCCAA GCAACCCCTC TGCATCTAAA GGCCGCTTGA TGAGATGTGT ACGCTGCCCA 
GTTGCATACC ATGCAAATGA TTTCTGCCTG CCAGCAGGAG CAGTTACATT GGCTTCCAAC 
AGTATTATTT GCCCCAACCA CTTCACCCCA AGGCGTGGAT GCAAGAACCA TGAGCATGTA 
AATGTCAGCT GGTGTTTTGT ATGCTCAGAA GGTGGAAGCC TCCTTTGCTG TGAATCCTGT 
CCTGCTGCTT TTCACAGAGA ATGCTTAAAC ATTGATATGC CAGAGGGCAG CTGGTTCTGC 
AATGATTGCA AAGCTGGCAA AAAGCCACAT TACAAAGAAG TGGTGTGGGT GAAGGTGGGG 
AGATACAGGT GGTGGCCAGC AGAGGTTTGT CATCCAAAAA CCATTCCACC CAACATCCAG 
AAGATGAAGC ATGACATTGG CGAGTTCCCA GTTGTCTTCT TTGGTTCCAA AGACTATTTG 
TGGACACACC AGGCCCGAGT GTTCCCATAT ATGGAAGGAG ATGCTCTCAA CAAAGACAAA 
ATGAGCAAGG GAATGGATGC TGTTTACAAA AAGGGGCTGA TGGAAGCTGC AGAAAGGTTT 
GAAGAATTAA AGGCCCAGAA AGAAATGAGA CAGTTACAGG AAGATAAAAA GAATGACAAG 
AAACCTCCAC CATATAAACA CATAAAAGTC AACAGGCCGG TTGGAAAAGT TCAGATTTTA 
ACTGCAGATC TTTCCGAAAT CCCTCGTTGC AACTGCAAGG CCACTGATGA AAATCCATGT 
GGGCAGGATT CCGAGTGCAT CAACCGTATG CTGCTGTACG AGTGCCATCC CTCAGTGTGC 
CCAGCAGGGG AGCGCTGTCA GAATCAAGCC TTCTCCAAAC GCCAGTACCC AGAAGTTGAA 
ATCTTTCGAA CTCTAAGTCG TGGATGGGGG CTGCGGTGTA GGACTGACAT TAAAAAGGGA 
GAATTTGTGA ATGAATATGT GGGTGAAATG ATTGATGAAG AGGAATGCCG TGCTCGCATC 
CGTTACGCCC AAGAGCAAGA CATCACAAAC TTCTACATGC TCACACTAGA TAAGGACCGT 
GTGATTGATG CAGGGCCCAA AGGTAACTTT GCACGTTTTA TGAATCACTG CTGCCAGCCT 
AACTGCGAGA CTCAGAAATG GACTGTGAAT GGTGATACAC GAGTTGGGCT CTTTGCTCTG 
TGTGATATCA AAGCAGGGGT AGAGTTAACA TTTAACTACA ACTTGGAGTG TCTTGGGAAT 
GGAAAGACTG TTTGCAAATG CGGAGCACCA AACTGCAGTG GTTTCCTCGG AGTGCGCCCC 
AAGAATCAGC CAGTCTCATC TGAGGACAAG GGTAAAAAGC GGAAGCAGTA TGTGAAACGT 
AAAAAAAGTG AGGTAGTCAA AGAACATGAA GATGAGTGTT TCAGCTGTGG AGATGGAGGA 
CAGCTTGTAT CTTGCAAGAA ACCAGGTTGC CCTAAAGTAT ACCATGCAGA ATGCTTGAAA 
TTAACCCGTA GACCAGCTGG TAAGTGGGAG TGTCCTTGGC ATCAATGTGA CATCTGTCAC 
AAGGAGGCTG CTTCTCTATG TGAGATGTGT CCAAGCTCCT TCTGCAAGCA GCACCGTGAG 
GGAATGCTCT TCATCTCTAA GCTTGACGGA CGGTTATCCT GCACAGAGCA TGACCCATGT 
GGGCCTCATC CCTTAGAGCC AGGAGAAATT AGGGAGTATG ACTCTTCTAA TGAACAGAAC 
GATTCTGCTG GCCCCTCACT TCCAAAGAAG AAAACACTGG ACAGTGCAAA GGGCAAAACA 
GCATCTTCCA ATTCACACAA GAAAAATGTT TCAGTGACTG AGGTGTTGCA TACACCAACA 
ACCGCTGGTT CTACATCTAG TGGCAATGTT AGCAAGGAAA ATGTGGGGTC TGTTGAGCAA 
AAATCATTGT CTGCTGGAAA AATACTCCTT GCCTCTGCTG GGCAAAAGTG TACTACTGGC 
AGTAAGTTGT TACTTGCCTC AGCAATTCAG AAATCATTGC CTGCTGGGAA GGTAGTCTTG 
ACCTCCTTGG GGAAAAAATC ATTGCCTGCT GGTAAGGTAC TGTTGGCTTC CATTGGAAAG 
AAGCAACTGT CTGCTGGGAA AGTTTTGGTG ACCTCTCTTG GGAAGAATTC TCCATCTGTT 
GGTAGGATGA TTATATCCAC AGCAGGAAAG CATTCTTTAC CAGCAGGAAA AGTTTTGTTA 
GCATCAGCTG GAAAGTATAC AACAGCAGGT AAAGTTCTGT TGACTTCTGC CACCAACCAG 
CATTCAACTG CTGAAAAAAA CAAGGCTGAT AAACAGAATG ATGTTAAAGA AACCCAAAAT 
TCATTGGACC CTGACACTAA ACATCGCATA ACTAAAAAGC CGCTTAATAA ACCATCTAAA 
GATAAGTTAA ATACTACTTT AGAGCAACCA TTGACTCAAA ACCACACAAC TGGGATTCAG 
AAACCTGTAA GCCCATGCCA AGTAGAAAGA CCTTCTAACC CATCCCTCAC AGGGAGGCCT 
GAAAAGCCTA AAGATAAGCA TCAACCAGAC AGTGAAGTTC ATCCTAAGAT CCTTTTAAAG 
AGGTCAACCA TGCCAACCTC CCGAAGGTCC CTGAGCGCAC ATAAAGAGAG GTTTCAAATT 
TCAACCAAAA AAGAAGGGTC TCTTGAAAGC AGCCAGGTAG AGAACCATGC CACTCCAAGA 
AAAGATTCTT GTTCTCCAAC TGTAAAGGAG AAGCCCAGTG CACCAAGTGA GAGATCTTTA 
TCTACAGTAG AGTCCCCTTC TATCCCAGCA GCTCCAGAGA ACTCTTCAGG CAGAGCAATA 
TCATGTACAC TTGCCTCCTC AGAAAAAACA GTTAGCACTT CTGAAATGTC ACTCAGCTCT 
GTTGTACAAG AGAAGACTAG TTCCACTGAA CCAAACTCTT CTTCAATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912864.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912921.1 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X8, mRNA.

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CloneID	OXa07643
Clone ID Related Accession (Same CDS sequence)	XM_004912864.3 , XM_002936951.4 , XM_004912861.3 , XM_004912862.3 , XM_004912860.3 , XM_004912858.3 , XM_004912859.3 , XM_004912863.3 , XM_018092215.1
Accession Version	XM_004912864.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912864.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912921.1
CDS	89..7411
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQGPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPIAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIRAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912864.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912864.3

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_002936951.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936997.2 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X1, mRNA.

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CloneID	OXa07643
Clone ID Related Accession (Same CDS sequence)	XM_004912864.3 , XM_002936951.4 , XM_004912861.3 , XM_004912862.3 , XM_004912860.3 , XM_004912858.3 , XM_004912859.3 , XM_004912863.3 , XM_018092215.1
Accession Version	XM_002936951.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936951.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936997.2
CDS	314..7636
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQGPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPIAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIRAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_002936951.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936951.4

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912861.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912918.1 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X5, mRNA.

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CloneID	OXa07643
Clone ID Related Accession (Same CDS sequence)	XM_004912864.3 , XM_002936951.4 , XM_004912861.3 , XM_004912862.3 , XM_004912860.3 , XM_004912858.3 , XM_004912859.3 , XM_004912863.3 , XM_018092215.1
Accession Version	XM_004912861.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912861.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912918.1
CDS	123..7445
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQGPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPIAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIRAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912861.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912861.3

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912862.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912919.1 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X7, mRNA.

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CloneID	OXa07643
Clone ID Related Accession (Same CDS sequence)	XM_004912864.3 , XM_002936951.4 , XM_004912861.3 , XM_004912862.3 , XM_004912860.3 , XM_004912858.3 , XM_004912859.3 , XM_004912863.3 , XM_018092215.1
Accession Version	XM_004912862.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912862.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912919.1
CDS	603..7925
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQGPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPIAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIRAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912862.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912862.3

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912860.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912917.1 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X9, mRNA.

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CloneID	OXa07643
Clone ID Related Accession (Same CDS sequence)	XM_004912864.3 , XM_002936951.4 , XM_004912861.3 , XM_004912862.3 , XM_004912860.3 , XM_004912858.3 , XM_004912859.3 , XM_004912863.3 , XM_018092215.1
Accession Version	XM_004912860.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912860.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912917.1
CDS	89..7411
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQGPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPIAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIRAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912860.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912860.3

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912858.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912915.1 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X2, mRNA.

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CloneID	OXa07643
Clone ID Related Accession (Same CDS sequence)	XM_004912864.3 , XM_002936951.4 , XM_004912861.3 , XM_004912862.3 , XM_004912860.3 , XM_004912858.3 , XM_004912859.3 , XM_004912863.3 , XM_018092215.1
Accession Version	XM_004912858.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912858.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912915.1
CDS	350..7672
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQGPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPIAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIRAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912858.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912858.3

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912859.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912916.1 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X3, mRNA.

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CloneID	OXa07643
Clone ID Related Accession (Same CDS sequence)	XM_004912864.3 , XM_002936951.4 , XM_004912861.3 , XM_004912862.3 , XM_004912860.3 , XM_004912858.3 , XM_004912859.3 , XM_004912863.3 , XM_018092215.1
Accession Version	XM_004912859.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912859.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912916.1
CDS	101..7423
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQGPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPIAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIRAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912859.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912859.3

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912863.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912920.1 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X4, mRNA.

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CloneID	OXa07643
Clone ID Related Accession (Same CDS sequence)	XM_004912864.3 , XM_002936951.4 , XM_004912861.3 , XM_004912862.3 , XM_004912860.3 , XM_004912858.3 , XM_004912859.3 , XM_004912863.3 , XM_018092215.1
Accession Version	XM_004912863.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912863.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912920.1
CDS	114..7436
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQGPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPIAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIRAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912863.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912863.3

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_018092215.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947704.1 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X6, mRNA.

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CloneID	OXa07643
Clone ID Related Accession (Same CDS sequence)	XM_004912864.3 , XM_002936951.4 , XM_004912861.3 , XM_004912862.3 , XM_004912860.3 , XM_004912858.3 , XM_004912859.3 , XM_004912863.3 , XM_018092215.1
Accession Version	XM_018092215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947704.1
CDS	341..7663
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQGPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGTLQGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPIAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIRAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_018092215.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092215.1

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GTACGTTACA AGGCTATCAC ACCTGCTTTG TTTGTAAGGA ATCTGACCAA 
GGAGTGAAGC GCTGCATGCT GCCCCTCTGT GGCAAATATT ATCATGAAGA ATGTGCCCTT 
AAATACCCTC CTGCAACTCA ACAAAACAGA GGCTTCCGCT GTTCTCTTCA TATCTGTTCA 
ACCTGTTATG CCACCAATCC AAGCAACCCC TCTGCATCTA AAGGCCGCTT GATGAGATGT 
GTACGCTGCC CAATTGCATA CCATGCAAAT GATTTCTGCC TGCCAGCAGG AGCAGTTACA 
TTGGCTTCCA ACAGTATTAT TTGCCCCAAC CACTTCACCC CAAGGCGTGG ATGCAAGAAC 
CATGAGCATG TAAATGTCAG CTGGTGTTTT GTATGCTCAG AAGGTGGAAG CCTCCTTTGC 
TGTGAATCCT GTCCTGCTGC TTTTCACAGA GAATGCTTAA ACATTGATAT GCCAGAGGGC 
AGCTGGTTCT GCAATGATTG CAAAGCTGGC AAAAAGCCAC ATTACAAAGA AGTGGTGTGG 
GTGAAGGTGG GGAGATACAG GTGGTGGCCA GCAGAGGTTT GTCATCCAAA AACCATTCCA 
CCCAACATCC AGAAGATGAA GCATGACATT GGCGAGTTCC CAGTTGTCTT CTTTGGTTCC 
AAAGACTATT TGTGGACACA CCAGGCCCGA GTGTTCCCAT ATATGGAAGG AGATGCTCTC 
AACAAAGACA AAATGAGCAA GGGAATGGAT GCTGTTTACA AAAAGGGGCT GATGGAAGCT 
GCAGAAAGGT TTGAAGAATT AAAGGCCCAG AAAGAAATGA GACAGTTACA GGAAGATAAA 
AAGAATGACA AGAAACCTCC ACCATATAAA CACATAAAAG TCAACAGGCC GGTTGGAAAA 
GTTCAGATTT TAACTGCAGA TCTTTCCGAA ATCCCTCGTT GCAACTGCAA GGCCACTGAT 
GAAAATCCAT GTGGGCAGGA TTCCGAGTGC ATCAACCGTA TGCTGCTGTA CGAGTGCCAT 
CCCTCAGTGT GCCCAGCAGG GGAGCGCTGT CAGAATCAAG CCTTCTCCAA ACGCCAGTAC 
CCAGAAGTTG AAATCTTTCG AACTCTAAGT CGTGGATGGG GGCTGCGGTG TAGGACTGAC 
ATTAAAAAGG GAGAATTTGT GAATGAATAT GTGGGTGAAA TGATTGATGA AGAGGAATGC 
CGTGCTCGCA TCCGTTACGC CCAAGAGCAA GACATCACAA ACTTCTACAT GCTCACACTA 
GATAAGGACC GTGTGATTGA TGCAGGGCCC AAAGGTAACT TTGCACGTTT TATGAATCAC 
TGCTGCCAGC CTAACTGCGA GACTCAGAAA TGGACTGTGA ATGGTGATAC ACGAGTTGGG 
CTCTTTGCTC TGTGTGATAT CAAAGCAGGG GTAGAGTTAA CATTTAACTA CAACTTGGAG 
TGTCTTGGGA ATGGAAAGAC TGTTTGCAAA TGCGGAGCAC CAAACTGCAG TGGTTTCCTC 
GGAGTGCGCC CCAAGAATCA GCCAGTCTCA TCTGAGGACA AGGGTAAAAA GCGGAAGCAG 
TATGTGAAAC GTAAAAAAAG TGAGGTAGTC AAAGAACATG AAGATGAGTG TTTCAGCTGT 
GGAGATGGAG GACAGCTTGT ATCTTGCAAG AAACCAGGTT GCCCTAAAGT ATACCATGCA 
GAATGCTTGA AATTAACCCG TAGACCAGCT GGTAAGTGGG AGTGTCCTTG GCATCAATGT 
GACATCTGTC ACAAGGAGGC TGCTTCTCTA TGTGAGATGT GTCCAAGCTC CTTCTGCAAG 
CAGCACCGTG AGGGAATGCT CTTCATCTCT AAGCTTGACG GACGGTTATC CTGCACAGAG 
CATGACCCAT GTGGGCCTCA TCCCTTAGAG CCAGGAGAAA TTAGGGAGTA TGACTCTTCT 
AATGAACAGA ACGATTCTGC TGGCCCCTCA CTTCCAAAGA AGAAAACACT GGACAGTGCA 
AAGGGCAAAA CAGCATCTTC CAATTCACAC AAGAAAAATG TTTCAGTGAC TGAGGTGTTG 
CATACACCAA CAACCGCTGG TTCTACATCT AGTGGCAATG TTAGCAAGGA AAATGTGGGG 
TCTGTTGAGC AAAAATCATT GTCTGCTGGA AAAATACTCC TTGCCTCTGC TGGGCAAAAG 
TGTACTACTG GCAGTAAGTT GTTACTTGCC TCAGCAATTC AGAAATCATT GCCTGCTGGG 
AAGGTAGTCT TGACCTCCTT GGGGAAAAAA TCATTGCCTG CTGGTAAGGT ACTGTTGGCT 
TCCATTGGAA AGAAGCAACT GTCTGCTGGG AAAGTTTTGG TGACCTCTCT TGGGAAGAAT 
TCTCCATCTG TTGGTAGGAT GATTATATCC ACAGCAGGAA AGCATTCTTT ACCAGCAGGA 
AAAGTTTTGT TAGCATCAGC TGGAAAGTAT ACAACAGCAG GTAAAGTTCT GTTGACTTCT 
GCCACCAACC AGCATTCAAC TGCTGAAAAA AACAAGGCTG ATAAACAGAA TGATGTTAAA 
GAAACCCAAA ATTCATTGGA CCCTGACACT AAACATCGCA TAACTAAAAA GCCGCTTAAT 
AAACCATCTA AAGATAAGTT AAATACTACT TTAGAGCAAC CATTGACTCA AAACCACACA 
ACTGGGATTC AGAAACCTGT AAGCCCATGC CAAGTAGAAA GACCTTCTAA CCCATCCCTC 
ACAGGGAGGC CTGAAAAGCC TAAAGATAAG CATCAACCAG ACAGTGAAGT TCATCCTAAG 
ATCCTTTTAA AGAGGTCAAC CATGCCAACC TCCCGAAGGT CCCTGAGCGC ACATAAAGAG 
AGGTTTCAAA TTTCAACCAA AAAAGAAGGG TCTCTTGAAA GCAGCCAGGT AGAGAACCAT 
GCCACTCCAA GAAAAGATTC TTGTTCTCCA ACTGTAAAGG AGAAGCCCAG TGCACCAAGT 
GAGAGATCTT TATCTACAGT AGAGTCCCCT TCTATCCGAG CAGCTCCAGA GAACTCTTCA 
GGCAGAGCAA TATCATGTAC ACTTGCCTCC TCAGAAAAAA CAGTTAGCAC TTCTGAAATG 
TCACTCAGCT CTGTTGTACA AGAGAAGACT AGTTCCACTG AACCAAACTC TTCTTCAATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsd1 ( XM_004912865.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912922.1 Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X10, mRNA.

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CloneID	OXa07644
Clone ID Related Accession (Same CDS sequence)	XM_004912865.3
Accession Version	XM_004912865.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GCTATCACAC CTGCTTTGTT TGTAAGGAAT CTGACCAAGG AGTGAAGCGC 
TGCATGCTGC CCCTCTGTGG CAAATATTAT CATGAAGAAT GTGCCCTTAA ATACCCTCCT 
GCAACTCAAC AAAACAGAGG CTTCCGCTGT TCTCTTCATA TCTGTTCAAC CTGTTATGCC 
ACCAATCCAA GCAACCCCTC TGCATCTAAA GGCCGCTTGA TGAGATGTGT ACGCTGCCCA 
ATTGCATACC ATGCAAATGA TTTCTGCCTG CCAGCAGGAG CAGTTACATT GGCTTCCAAC 
AGTATTATTT GCCCCAACCA CTTCACCCCA AGGCGTGGAT GCAAGAACCA TGAGCATGTA 
AATGTCAGCT GGTGTTTTGT ATGCTCAGAA GGTGGAAGCC TCCTTTGCTG TGAATCCTGT 
CCTGCTGCTT TTCACAGAGA ATGCTTAAAC ATTGATATGC CAGAGGGCAG CTGGTTCTGC 
AATGATTGCA AAGCTGGCAA AAAGCCACAT TACAAAGAAG TGGTGTGGGT GAAGGTGGGG 
AGATACAGGT GGTGGCCAGC AGAGGTTTGT CATCCAAAAA CCATTCCACC CAACATCCAG 
AAGATGAAGC ATGACATTGG CGAGTTCCCA GTTGTCTTCT TTGGTTCCAA AGACTATTTG 
TGGACACACC AGGCCCGAGT GTTCCCATAT ATGGAAGGAG ATGCTCTCAA CAAAGACAAA 
ATGAGCAAGG GAATGGATGC TGTTTACAAA AAGGGGCTGA TGGAAGCTGC AGAAAGGTTT 
GAAGAATTAA AGGCCCAGAA AGAAATGAGA CAGTTACAGG AAGATAAAAA GAATGACAAG 
AAACCTCCAC CATATAAACA CATAAAAGTC AACAGGCCGG TTGGAAAAGT TCAGATTTTA 
ACTGCAGATC TTTCCGAAAT CCCTCGTTGC AACTGCAAGG CCACTGATGA AAATCCATGT 
GGGCAGGATT CCGAGTGCAT CAACCGTATG CTGCTGTACG AGTGCCATCC CTCAGTGTGC 
CCAGCAGGGG AGCGCTGTCA GAATCAAGCC TTCTCCAAAC GCCAGTACCC AGAAGTTGAA 
ATCTTTCGAA CTCTAAGTCG TGGATGGGGG CTGCGGTGTA GGACTGACAT TAAAAAGGGA 
GAATTTGTGA ATGAATATGT GGGTGAAATG ATTGATGAAG AGGAATGCCG TGCTCGCATC 
CGTTACGCCC AAGAGCAAGA CATCACAAAC TTCTACATGC TCACACTAGA TAAGGACCGT 
GTGATTGATG CAGGGCCCAA AGGTAACTTT GCACGTTTTA TGAATCACTG CTGCCAGCCT 
AACTGCGAGA CTCAGAAATG GACTGTGAAT GGTGATACAC GAGTTGGGCT CTTTGCTCTG 
TGTGATATCA AAGCAGGGGT AGAGTTAACA TTTAACTACA ACTTGGAGTG TCTTGGGAAT 
GGAAAGACTG TTTGCAAATG CGGAGCACCA AACTGCAGTG GTTTCCTCGG AGTGCGCCCC 
AAGAATCAGC CAGTCTCATC TGAGGACAAG GGTAAAAAGC GGAAGCAGTA TGTGAAACGT 
AAAAAAAGTG AGGTAGTCAA AGAACATGAA GATGAGTGTT TCAGCTGTGG AGATGGAGGA 
CAGCTTGTAT CTTGCAAGAA ACCAGGTTGC CCTAAAGTAT ACCATGCAGA ATGCTTGAAA 
TTAACCCGTA GACCAGCTGG TAAGTGGGAG TGTCCTTGGC ATCAATGTGA CATCTGTCAC 
AAGGAGGCTG CTTCTCTATG TGAGATGTGT CCAAGCTCCT TCTGCAAGCA GCACCGTGAG 
GGAATGCTCT TCATCTCTAA GCTTGACGGA CGGTTATCCT GCACAGAGCA TGACCCATGT 
GGGCCTCATC CCTTAGAGCC AGGAGAAATT AGGGAGTATG ACTCTTCTAA TGAACAGAAC 
GATTCTGCTG GCCCCTCACT TCCAAAGAAG AAAACACTGG ACAGTGCAAA GGGCAAAACA 
GCATCTTCCA ATTCACACAA GAAAAATGTT TCAGTGACTG AGGTGTTGCA TACACCAACA 
ACCGCTGGTT CTACATCTAG TGGCAATGTT AGCAAGGAAA ATGTGGGGTC TGTTGAGCAA 
AAATCATTGT CTGCTGGAAA AATACTCCTT GCCTCTGCTG GGCAAAAGTG TACTACTGGC 
AGTAAGTTGT TACTTGCCTC AGCAATTCAG AAATCATTGC CTGCTGGGAA GGTAGTCTTG 
ACCTCCTTGG GGAAAAAATC ATTGCCTGCT GGTAAGGTAC TGTTGGCTTC CATTGGAAAG 
AAGCAACTGT CTGCTGGGAA AGTTTTGGTG ACCTCTCTTG GGAAGAATTC TCCATCTGTT 
GGTAGGATGA TTATATCCAC AGCAGGAAAG CATTCTTTAC CAGCAGGAAA AGTTTTGTTA 
GCATCAGCTG GAAAGTATAC AACAGCAGGT AAAGTTCTGT TGACTTCTGC CACCAACCAG 
CATTCAACTG CTGAAAAAAA CAAGGCTGAT AAACAGAATG ATGTTAAAGA AACCCAAAAT 
TCATTGGACC CTGACACTAA ACATCGCATA ACTAAAAAGC CGCTTAATAA ACCATCTAAA 
GATAAGTTAA ATACTACTTT AGAGCAACCA TTGACTCAAA ACCACACAAC TGGGATTCAG 
AAACCTGTAA GCCCATGCCA AGTAGAAAGA CCTTCTAACC CATCCCTCAC AGGGAGGCCT 
GAAAAGCCTA AAGATAAGCA TCAACCAGAC AGTGAAGTTC ATCCTAAGAT CCTTTTAAAG 
AGGTCAACCA TGCCAACCTC CCGAAGGTCC CTGAGCGCAC ATAAAGAGAG GTTTCAAATT 
TCAACCAAAA AAGAAGGGTC TCTTGAAAGC AGCCAGGTAG AGAACCATGC CACTCCAAGA 
AAAGATTCTT GTTCTCCAAC TGTAAAGGAG AAGCCCAGTG CACCAAGTGA GAGATCTTTA 
TCTACAGTAG AGTCCCCTTC TATCCGAGCA GCTCCAGAGA ACTCTTCAGG CAGAGCAATA 
TCATGTACAC TTGCCTCCTC AGAAAAAACA GTTAGCACTT CTGAAATGTC ACTCAGCTCT 
GTTGTACAAG AGAAGACTAG TTCCACTGAA CCAAACTCTT CTTCAATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912922.1
CDS	313..7623
Translation	MDEACELRSTTDYLNSVDPNAKSKPCVGDRGILELPNGCSVPLSSPTQSGKLGYGQGPSSHYIPLRRLQDLASMISIEGMNGSQNMEANGSQPNPQPYFFTSNEPRGHASHQDSTRQFSQDLTMKPKNVKNGMHPYDSFCHVDMEEPNYAIEQLNYNHTGPGFDVPGYGQQPPVEQNILPVGENQSKINPTPQFGSELEDLVPLAWSSNGEDPMENENLNTKTSFLSMIRDAEIPNGAPINTTPENAIHGDLDHSTILHLDGRLSNQGPLTAEHTEDESLSVEEELDDLDSHNELSPDRSGLQFLPRRKKPALLRYDEGDVVWAKFNRRPWWPCRICANSEPEGEPQHKAFSKRLPRNYYVETLGELTEKIWVPAKALVPYRGAHQFEGLPEFRRTQRQEDKSFKNKVPLRLLSLWQSSIEHAQDCLSRGAERYESIEEVEGNKQIANYGAKESSGPIKNSFNGFVKSSLCSIDLSCTKTDSKKQHRRLKPVKHRKVELRLSDVSTDDSRTTEEPGSPEFFSSQTCESKFGMAPSQQESCISKQISKEVYNISQEKNVTLSAKKWNKNDELVEQSLGLERTKAQTDNGQEKSEKPLMGHKIEAKQKDFRLKQSVSSVQKERSAGTTKKRKRLRIHSELSRSSKRLVASSLDSSTNVESSNEFSLSKDWVTEDVPDGNGHSVDKNKYSPSLTNVSLSDSCENLHNSLLESNAPSPSQHSIKDFPKALTPESKNCLVHDSATQNSQSKNILDSKLGHKPAKASLGKTGRPEAMNLLSNMHEKSCDSMKNETKLVNSVISGLKELSSRSLSEDGAESTVSKPSTPLLFASAPGQNRIPLEPDYKFSNLLMMLKDMHDTKTKERKLMTGQNTVSSCSDPCEDSIKGSPDIVPDVGGISGNHTHSNEVGMVKEPNNESKLGATGQNKIVCNRKSSSKKNSNFQKKPPMEPKKVSKYKKSMISAQNVNASSECTELTTNSYNTSVQLHGECPSKSITHPYNCSEVGAGYPEETGPGSEQSSECSQWNESQKYVAPKKRWQKIKHNGPESSTNLLNKASTKSSLCKKEQDVLEVPKQLQDHEVTFGEPELSSIAAQPEAPKRGSNFGKKYKKRKRFTRRSGDHVKTSTPKINKQKKLGVSLQLEVNKQKNLKLILPAVDFLSEINSKATSSSQFSKLAEAYSTETKNPDNEEKSDSSEKEDHGLASPEQSLSDGFSEPSSITAQSQVNPVRENRRKSKLIPRVCEFDDLDEQLGLLEEDSKPPAKIAKTTSFKQNVLKQSFRYRAASRRLPENRRRRKRIRLAGKFKKKRNLISRQSSYPGEEPHENGVASHSDTAEEGLEHELTGASLKRNQGERSGGGAAMKENVCQVCEKPGELLLCEAQCCGAFHLQCLGMEAMPQGKFVCTECSSGYHTCFVCKESDQGVKRCMLPLCGKYYHEECALKYPPATQQNRGFRCSLHICSTCYATNPSNPSASKGRLMRCVRCPIAYHANDFCLPAGAVTLASNSIICPNHFTPRRGCKNHEHVNVSWCFVCSEGGSLLCCESCPAAFHRECLNIDMPEGSWFCNDCKAGKKPHYKEVVWVKVGRYRWWPAEVCHPKTIPPNIQKMKHDIGEFPVVFFGSKDYLWTHQARVFPYMEGDALNKDKMSKGMDAVYKKGLMEAAERFEELKAQKEMRQLQEDKKNDKKPPPYKHIKVNRPVGKVQILTADLSEIPRCNCKATDENPCGQDSECINRMLLYECHPSVCPAGERCQNQAFSKRQYPEVEIFRTLSRGWGLRCRTDIKKGEFVNEYVGEMIDEEECRARIRYAQEQDITNFYMLTLDKDRVIDAGPKGNFARFMNHCCQPNCETQKWTVNGDTRVGLFALCDIKAGVELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPVSSEDKGKKRKQYVKRKKSEVVKEHEDECFSCGDGGQLVSCKKPGCPKVYHAECLKLTRRPAGKWECPWHQCDICHKEAASLCEMCPSSFCKQHREGMLFISKLDGRLSCTEHDPCGPHPLEPGEIREYDSSNEQNDSAGPSLPKKKTLDSAKGKTASSNSHKKNVSVTEVLHTPTTAGSTSSGNVSKENVGSVEQKSLSAGKILLASAGQKCTTGSKLLLASAIQKSLPAGKVVLTSLGKKSLPAGKVLLASIGKKQLSAGKVLVTSLGKNSPSVGRMIISTAGKHSLPAGKVLLASAGKYTTAGKVLLTSATNQHSTAEKNKADKQNDVKETQNSLDPDTKHRITKKPLNKPSKDKLNTTLEQPLTQNHTTGIQKPVSPCQVERPSNPSLTGRPEKPKDKHQPDSEVHPKILLKRSTMPTSRRSLSAHKERFQISTKKEGSLESSQVENHATPRKDSCSPTVKEKPSAPSERSLSTVESPSIRAAPENSSGRAISCTLASSEKTVSTSEMSLSSVVQEKTSSTEPNSSSI

Target ORF information:

RefSeq Version	XM_004912865.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear receptor binding SET domain protein 1 (nsd1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912865.3

ORF Insert Sequence:

ATGGATGAAG CCTGTGAACT AAGGAGTACT ACAGACTATT TGAACTCAGT GGATCCTAAT 
GCCAAAAGCA AACCGTGCGT GGGTGACAGG GGCATTTTGG AACTACCAAA TGGGTGTAGC 
GTTCCACTGA GCTCACCCAC GCAAAGTGGC AAATTGGGTT ATGGGCAGGG TCCCTCCTCC 
CACTACATTC CTCTGCGCAG ACTCCAGGAT CTGGCCTCCA TGATAAGCAT CGAAGGCATG 
AATGGATCAC AAAACATGGA GGCAAACGGA TCTCAACCAA ATCCTCAGCC CTATTTTTTT 
ACTTCAAATG AGCCAAGAGG TCATGCCAGT CATCAGGATT CTACTCGTCA ATTTTCACAA 
GACCTCACCA TGAAACCCAA AAATGTAAAG AATGGGATGC ATCCTTATGA TTCTTTTTGT 
CATGTTGATA TGGAAGAGCC AAATTATGCT ATAGAACAGT TAAATTACAA TCACACTGGG 
CCAGGTTTTG ATGTGCCAGG CTATGGGCAA CAGCCACCGG TGGAGCAAAA TATTTTACCT 
GTTGGGGAGA ATCAATCTAA AATTAACCCC ACACCTCAGT TTGGATCAGA ATTAGAGGAT 
TTAGTTCCAC TTGCTTGGTC TAGTAATGGA GAGGACCCAA TGGAAAATGA AAATTTAAAC 
ACAAAAACAT CTTTTTTATC AATGATTCGG GATGCTGAAA TTCCAAACGG CGCACCTATT 
AATACTACTC CAGAAAATGC AATTCATGGG GATTTGGATC ACAGTACAAT TTTACATTTA 
GATGGTAGAC TAAGTAACCA AGGACCGCTA ACAGCGGAAC ACACTGAAGA TGAATCGTTG 
TCTGTAGAAG AAGAGCTGGA CGATTTGGAT TCACATAATG AATTATCTCC GGATCGATCA 
GGATTACAAT TTTTACCAAG AAGGAAAAAA CCTGCTCTTT TAAGGTATGA CGAGGGGGAT 
GTCGTCTGGG CCAAATTCAA CAGGCGCCCA TGGTGGCCAT GCAGAATCTG TGCCAATTCA 
GAGCCAGAGG GAGAACCCCA ACATAAAGCA TTTAGCAAAA GGCTACCCCG GAATTATTAT 
GTTGAGACTT TGGGAGAGTT AACTGAGAAG ATCTGGGTCC CTGCAAAAGC ACTTGTACCG 
TACAGGGGGG CACACCAATT TGAAGGATTG CCAGAATTCC GGCGGACTCA AAGGCAGGAA 
GATAAATCTT TTAAGAATAA GGTCCCCTTG AGACTTTTAT CGCTATGGCA GTCCAGCATT 
GAACATGCCC AAGACTGTCT CTCCAGAGGG GCTGAGAGGT ATGAAAGCAT AGAAGAGGTA 
GAAGGTAACA AGCAAATAGC AAATTATGGT GCAAAAGAAT CCAGTGGGCC AATTAAGAAT 
TCATTCAATG GATTTGTAAA ATCTTCCTTG TGTAGCATTG ACCTGTCATG CACTAAAACT 
GACTCAAAAA AACAACATAG GCGTTTAAAG CCTGTAAAAC ATAGAAAAGT GGAATTACGG 
CTATCTGATG TTTCAACCGA TGATTCAAGA ACAACAGAGG AACCAGGGTC TCCAGAATTT 
TTTAGCAGTC AAACTTGTGA ATCAAAATTT GGAATGGCAC CATCTCAACA GGAGAGCTGT 
ATATCTAAAC AAATATCTAA AGAGGTCTAC AACATAAGTC AGGAGAAAAA CGTAACGTTA 
TCTGCAAAGA AATGGAATAA AAATGATGAA CTTGTAGAGC AAAGTTTGGG TTTAGAGAGA 
ACAAAGGCTC AAACTGACAA TGGACAAGAA AAATCAGAAA AACCTTTAAT GGGACACAAA 
ATTGAAGCTA AACAAAAGGA CTTTAGGCTT AAACAAAGTG TAAGCTCTGT ACAGAAGGAA 
AGATCTGCTG GCACAACTAA AAAAAGGAAG CGACTACGAA TTCATTCTGA ATTAAGCCGC 
TCAAGCAAGC GGCTGGTTGC ATCTTCATTA GACTCTAGCA CAAATGTTGA ATCTAGTAAT 
GAGTTTTCTC TTTCGAAAGA CTGGGTTACT GAAGATGTGC CAGATGGGAA TGGGCACAGT 
GTGGATAAAA ACAAATACAG TCCGTCGCTT ACTAATGTTT CTTTATCTGA CTCTTGTGAG 
AACCTGCACA ATTCTTTATT GGAAAGCAAT GCCCCCAGCC CAAGTCAACA TTCAATAAAA 
GATTTTCCCA AGGCTTTGAC TCCTGAAAGC AAAAACTGTT TAGTCCATGA TTCGGCAACC 
CAAAATTCAC AGTCTAAAAA CATTCTGGAT TCGAAACTTG GCCACAAACC TGCAAAGGCT 
TCTCTTGGTA AAACTGGACG TCCTGAAGCA ATGAATCTGT TAAGTAATAT GCATGAAAAG 
TCATGCGATT CAATGAAGAA TGAGACAAAA TTAGTAAACA GTGTTATATC TGGTCTTAAA 
GAACTGTCCT CCAGGTCTCT CAGTGAGGAT GGAGCCGAAA GTACAGTATC CAAACCATCA 
ACACCTTTGC TTTTTGCTTC AGCTCCAGGG CAAAATCGAA TACCTTTGGA ACCAGATTAC 
AAATTTAGCA ACCTGCTAAT GATGCTGAAA GACATGCATG ATACTAAAAC AAAGGAAAGG 
AAGCTAATGA CTGGACAAAA TACTGTATCT AGCTGCAGCG ACCCATGTGA AGACTCTATT 
AAAGGTAGCC CTGATATTGT ACCTGATGTA GGTGGTATTT CTGGTAACCA CACACACAGT 
AATGAAGTTG GTATGGTAAA AGAACCCAAC AATGAAAGTA AACTGGGAGC AACGGGTCAA 
AATAAGATCG TCTGTAACAG AAAGTCAAGT TCTAAAAAGA ACTCCAATTT CCAGAAAAAG 
CCACCCATGG AGCCCAAAAA AGTGTCAAAG TATAAAAAGA GCATGATTTC TGCACAAAAT 
GTCAATGCAT CTTCTGAATG TACAGAACTA ACTACAAATT CATATAATAC CTCAGTGCAA 
TTACATGGTG AGTGTCCTAG TAAAAGCATA ACCCATCCTT ATAATTGCAG TGAAGTTGGT 
GCAGGTTACC CAGAGGAAAC TGGACCTGGC TCAGAACAGA GCAGTGAATG CTCTCAGTGG 
AATGAGTCAC AAAAGTATGT TGCGCCTAAA AAGAGGTGGC AGAAAATCAA ACATAATGGC 
CCAGAAAGCA GCACAAACCT TTTAAATAAG GCCTCTACTA AGTCCAGTTT GTGCAAGAAG 
GAGCAAGATG TTTTAGAAGT TCCTAAACAG CTGCAGGATC ATGAAGTGAC ATTTGGAGAA 
CCTGAGCTTT CTAGTATAGC AGCACAGCCA GAAGCTCCAA AGAGAGGGTC AAATTTTGGA 
AAGAAATATA AAAAAAGGAA GAGATTTACC AGGCGTTCAG GTGATCATGT TAAGACAAGC 
ACCCCCAAAA TTAACAAGCA GAAGAAGTTG GGTGTCTCTC TCCAGTTGGA AGTCAACAAG 
CAAAAGAACC TTAAATTAAT CCTCCCTGCT GTAGATTTTT TGAGTGAAAT AAATAGTAAA 
GCCACATCAT CTTCCCAATT TTCCAAACTG GCTGAGGCTT ATTCTACGGA GACAAAAAAT 
CCAGATAATG AAGAAAAGAG TGATTCTTCA GAAAAAGAGG ATCATGGTCT TGCTTCACCA 
GAGCAATCTT TGTCAGATGG CTTTTCTGAA CCTTCTAGTA TAACCGCTCA ATCGCAAGTC 
AATCCAGTTC GAGAAAACAG GCGTAAGAGC AAATTAATTC CGAGGGTGTG TGAATTCGAT 
GACCTTGATG AACAGCTTGG GCTTTTGGAG GAAGATTCAA AACCCCCTGC TAAGATTGCC 
AAGACTACTT CATTCAAGCA AAACGTACTA AAGCAGTCCT TCAGGTACAG AGCAGCATCC 
AGAAGGCTGC CAGAGAATCG GAGAAGGCGT AAAAGGATTC GGCTTGCGGG AAAGTTCAAG 
AAGAAGAGGA ACCTGATTTC CCGACAGTCT TCCTACCCTG GGGAAGAGCC TCATGAGAAT 
GGCGTTGCCA GCCATAGTGA CACTGCTGAG GAAGGACTAG AACATGAGCT GACAGGAGCC 
TCCTTAAAGA GGAATCAAGG AGAACGTAGT GGTGGGGGAG CTGCAATGAA AGAAAATGTA 
TGCCAGGTGT GTGAGAAACC TGGAGAATTG CTGCTGTGTG AGGCCCAGTG CTGTGGAGCA 
TTCCATCTGC AGTGCCTTGG CATGGAGGCC ATGCCTCAGG GAAAGTTTGT CTGCACAGAG 
TGCTCCTCGG GCTATCACAC CTGCTTTGTT TGTAAGGAAT CTGACCAAGG AGTGAAGCGC 
TGCATGCTGC CCCTCTGTGG CAAATATTAT CATGAAGAAT GTGCCCTTAA ATACCCTCCT 
GCAACTCAAC AAAACAGAGG CTTCCGCTGT TCTCTTCATA TCTGTTCAAC CTGTTATGCC 
ACCAATCCAA GCAACCCCTC TGCATCTAAA GGCCGCTTGA TGAGATGTGT ACGCTGCCCA 
ATTGCATACC ATGCAAATGA TTTCTGCCTG CCAGCAGGAG CAGTTACATT GGCTTCCAAC 
AGTATTATTT GCCCCAACCA CTTCACCCCA AGGCGTGGAT GCAAGAACCA TGAGCATGTA 
AATGTCAGCT GGTGTTTTGT ATGCTCAGAA GGTGGAAGCC TCCTTTGCTG TGAATCCTGT 
CCTGCTGCTT TTCACAGAGA ATGCTTAAAC ATTGATATGC CAGAGGGCAG CTGGTTCTGC 
AATGATTGCA AAGCTGGCAA AAAGCCACAT TACAAAGAAG TGGTGTGGGT GAAGGTGGGG 
AGATACAGGT GGTGGCCAGC AGAGGTTTGT CATCCAAAAA CCATTCCACC CAACATCCAG 
AAGATGAAGC ATGACATTGG CGAGTTCCCA GTTGTCTTCT TTGGTTCCAA AGACTATTTG 
TGGACACACC AGGCCCGAGT GTTCCCATAT ATGGAAGGAG ATGCTCTCAA CAAAGACAAA 
ATGAGCAAGG GAATGGATGC TGTTTACAAA AAGGGGCTGA TGGAAGCTGC AGAAAGGTTT 
GAAGAATTAA AGGCCCAGAA AGAAATGAGA CAGTTACAGG AAGATAAAAA GAATGACAAG 
AAACCTCCAC CATATAAACA CATAAAAGTC AACAGGCCGG TTGGAAAAGT TCAGATTTTA 
ACTGCAGATC TTTCCGAAAT CCCTCGTTGC AACTGCAAGG CCACTGATGA AAATCCATGT 
GGGCAGGATT CCGAGTGCAT CAACCGTATG CTGCTGTACG AGTGCCATCC CTCAGTGTGC 
CCAGCAGGGG AGCGCTGTCA GAATCAAGCC TTCTCCAAAC GCCAGTACCC AGAAGTTGAA 
ATCTTTCGAA CTCTAAGTCG TGGATGGGGG CTGCGGTGTA GGACTGACAT TAAAAAGGGA 
GAATTTGTGA ATGAATATGT GGGTGAAATG ATTGATGAAG AGGAATGCCG TGCTCGCATC 
CGTTACGCCC AAGAGCAAGA CATCACAAAC TTCTACATGC TCACACTAGA TAAGGACCGT 
GTGATTGATG CAGGGCCCAA AGGTAACTTT GCACGTTTTA TGAATCACTG CTGCCAGCCT 
AACTGCGAGA CTCAGAAATG GACTGTGAAT GGTGATACAC GAGTTGGGCT CTTTGCTCTG 
TGTGATATCA AAGCAGGGGT AGAGTTAACA TTTAACTACA ACTTGGAGTG TCTTGGGAAT 
GGAAAGACTG TTTGCAAATG CGGAGCACCA AACTGCAGTG GTTTCCTCGG AGTGCGCCCC 
AAGAATCAGC CAGTCTCATC TGAGGACAAG GGTAAAAAGC GGAAGCAGTA TGTGAAACGT 
AAAAAAAGTG AGGTAGTCAA AGAACATGAA GATGAGTGTT TCAGCTGTGG AGATGGAGGA 
CAGCTTGTAT CTTGCAAGAA ACCAGGTTGC CCTAAAGTAT ACCATGCAGA ATGCTTGAAA 
TTAACCCGTA GACCAGCTGG TAAGTGGGAG TGTCCTTGGC ATCAATGTGA CATCTGTCAC 
AAGGAGGCTG CTTCTCTATG TGAGATGTGT CCAAGCTCCT TCTGCAAGCA GCACCGTGAG 
GGAATGCTCT TCATCTCTAA GCTTGACGGA CGGTTATCCT GCACAGAGCA TGACCCATGT 
GGGCCTCATC CCTTAGAGCC AGGAGAAATT AGGGAGTATG ACTCTTCTAA TGAACAGAAC 
GATTCTGCTG GCCCCTCACT TCCAAAGAAG AAAACACTGG ACAGTGCAAA GGGCAAAACA 
GCATCTTCCA ATTCACACAA GAAAAATGTT TCAGTGACTG AGGTGTTGCA TACACCAACA 
ACCGCTGGTT CTACATCTAG TGGCAATGTT AGCAAGGAAA ATGTGGGGTC TGTTGAGCAA 
AAATCATTGT CTGCTGGAAA AATACTCCTT GCCTCTGCTG GGCAAAAGTG TACTACTGGC 
AGTAAGTTGT TACTTGCCTC AGCAATTCAG AAATCATTGC CTGCTGGGAA GGTAGTCTTG 
ACCTCCTTGG GGAAAAAATC ATTGCCTGCT GGTAAGGTAC TGTTGGCTTC CATTGGAAAG 
AAGCAACTGT CTGCTGGGAA AGTTTTGGTG ACCTCTCTTG GGAAGAATTC TCCATCTGTT 
GGTAGGATGA TTATATCCAC AGCAGGAAAG CATTCTTTAC CAGCAGGAAA AGTTTTGTTA 
GCATCAGCTG GAAAGTATAC AACAGCAGGT AAAGTTCTGT TGACTTCTGC CACCAACCAG 
CATTCAACTG CTGAAAAAAA CAAGGCTGAT AAACAGAATG ATGTTAAAGA AACCCAAAAT 
TCATTGGACC CTGACACTAA ACATCGCATA ACTAAAAAGC CGCTTAATAA ACCATCTAAA 
GATAAGTTAA ATACTACTTT AGAGCAACCA TTGACTCAAA ACCACACAAC TGGGATTCAG 
AAACCTGTAA GCCCATGCCA AGTAGAAAGA CCTTCTAACC CATCCCTCAC AGGGAGGCCT 
GAAAAGCCTA AAGATAAGCA TCAACCAGAC AGTGAAGTTC ATCCTAAGAT CCTTTTAAAG 
AGGTCAACCA TGCCAACCTC CCGAAGGTCC CTGAGCGCAC ATAAAGAGAG GTTTCAAATT 
TCAACCAAAA AAGAAGGGTC TCTTGAAAGC AGCCAGGTAG AGAACCATGC CACTCCAAGA 
AAAGATTCTT GTTCTCCAAC TGTAAAGGAG AAGCCCAGTG CACCAAGTGA GAGATCTTTA 
TCTACAGTAG AGTCCCCTTC TATCCGAGCA GCTCCAGAGA ACTCTTCAGG CAGAGCAATA 
TCATGTACAC TTGCCTCCTC AGAAAAAACA GTTAGCACTT CTGAAATGTC ACTCAGCTCT 
GTTGTACAAG AGAAGACTAG TTCCACTGAA CCAAACTCTT CTTCAATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	nsd1
Entrez Gene ID	100145675
Full Name	nuclear receptor binding SET domain protein 1
Synonyms	ara267,kmt3b,sotos,sto
General protein information	Preferred Names nuclear receptor binding SET domain protein 1 Names histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

nsd1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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