iars2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	iars2
Entrez Gene ID	100145647
Full Name	isoleucyl-tRNA synthetase 2, mitochondrial
General protein information	Preferred Names isoleucyl-tRNA synthetase 2, mitochondrial Names isoleucine--tRNA ligase, mitochondrial
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001127043.1	NP_001120515.1	isoleucine--tRNA ligase, mitochondrial

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5414	NP_648837.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003156	XP_312846.5
Eremothecium gossypii	AGOS_AGL247C	NP_986420.1
Neurospora crassa	NCU00920	XP_964746.2
Arabidopsis thaliana (thale cress)	OVA2	NP_199714.2
Canis lupus familiaris (dog)	IARS2	XP_536121.3
Gallus gallus (chicken)	IARS2	NP_001006397.1
Saccharomyces cerevisiae (baker's yeast)	ISM1	NP_015285.1
Schizosaccharomyces pombe (fission yeast)	SPCC18B5.08c	NP_587938.1
Homo sapiens (human)	IARS2	NP_060530.3
Pan troglodytes (chimpanzee)	IARS2	XP_514209.2
	IARS2	XP_001099877.1
Danio rerio (zebrafish)	iars2	NP_001157270.1
Caenorhabditis elegans (roundworm)	iars-2	NP_001251317.1
Kluyveromyces lactis	KLLA0D11858g	XP_453590.1
Xenopus tropicalis (tropical clawed frog)	iars2	NP_001120515.1
Bos taurus (cattle)	IARS2	NP_001193724.1
Mus musculus (house mouse)	Iars2	NP_941055.1
Rattus norvegicus (Norway rat)	Iars2	XP_006250494.1
Magnaporthe oryzae (rice blast fungus)	MGG_01165	XP_003714047.1
Oryza sativa (rice)	Os02g0778200	NP_001048293.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following iars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the iars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa01919	NM_001127043.1 Latest version!	Xenopus tropicalis isoleucyl-tRNA synthetase 2, mitochondrial (iars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

iars2 ( NM_001127043.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001120515.1 Xenopus tropicalis isoleucyl-tRNA synthetase 2, mitochondrial (iars2), mRNA.

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CloneID	OXa01919
Clone ID Related Accession (Same CDS sequence)	NM_001127043.1
Accession Version	NM_001127043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	isoleucine--tRNA ligase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161415.1. ##Evidence-Data-START## Transcript exon combination :: BC161415.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCTGCGCC TTCTCCGGTG CCGGCTGCCA GTTCGGCGCT ACCGCTGGGG AGTAAGAGCC 
GCCTCTGAGA AGGCCAGCAG CAATGAGAGC CCCGGGAATA GGAAGTACCG GGGCACCGTG 
CTGCTCCCTC ACACTGACTT CCCTATCCGC CGGGGCCCGG GGGAGACAGC CAGCACCGAA 
CTACGGCTGC AGGAGGAAAG TGGATTTCTG CAACTCTATG CTTGGCAAAG GGAAAGGAAA 
ACCAAGAAGG AATTCAGTCT TCATGATGGA CCACCCTATG CCAATGGGGA CCCCCATGTG 
GGCCATGCTC TTAATAAGAT CCTCAAAGAT ATAACAAATC GTTTCCACAT GATGAGAGGC 
TACAAAGTGA GCTACGTGCC AGGATGGGAC TGCCATGGGC TGCCGATTGA ACTAAAAGCT 
CTGGCGGAAC TTGGAGAGGA AGCCGAAAAT ATGTCACCCA CTGAGATCAG GCGCAAAGCC 
AAGATGTTTG CAGAGAAAGC CATTGAAAAG CAGAAATCCT CCTTCATTCG CTGGGGTATC 
ATGGCAGACT GGGACAAGTG CTATTACACA TTCGATCCAA AGTATGAAGC AAAACAATTA 
GACATTTTCT ACAAGATGTA TGACAAGGGC CACATATACC AGGATTACAA GCCTGTCTTC 
TGGTCTCCAT CAACAAGAAC AGCCCTCGCA GAGGCAGAGT TGGAATACAA CCAGGAGCAC 
ATAAGTCGCT CAATATTTGC CAAATTCCCA ATGCACAAAG CTCCTCCAAA GCTGGCCTCC 
ATTATAGATG GATCCTCTGC AGTCAGCACT CTGGTTTGGA CGACCCAGCC TTGGACTATT 
CCTGCTAACC AGGCCATCTG CTATATGCCA GATACAGAGT ATGCCATAGC AAAGTGTGCT 
AATACTGGAG AACAGTACAT ATTAGCCACA GATAGAATTG AATCTACCGC TTTGTCCTTG 
GGTACCCAGC TTCAAGTCAT TTCAACCTTT AAAGGGCTAG ATCTGGAGGG CGGCATCTGT 
CTCCATCCCA CCATTGCTGA TCGCCTTTCT CCTCTCTTGC CTGCCAACCA CGTGACTATG 
GCAAAGGGCA CAGGCCTGGT ACACACTGCA CCGGCCCATG GCATTGAAGA CTACAGTGTT 
GCATCACATC ACAAGCTCCC AGTGGACTGT TTGGTGGATG AATCGGGGCA TTTTACTACT 
GGAGCTGGGG CTGAACTGGA AAATAAAGCT GTACTTACTG ATGGAAATGA GACAGTCATA 
CAGATGCTAC ATGCCAGCAA GTCTCTGCTG AAGGAGGAGA GTTTTGTACA CAGTTACCCC 
TATGACTGGA GAACAAAGAA ACCCGTGATA ATCCGTGCCA GTAAACAGTG GTTTGTCAAT 
ACAGCAAACA TTAAGGACAA AGCCCAGGAT GTGTTAAAGA AAGTGAAGTT TGTTCCCGCT 
TCAGCAGCTG CCAGAATGCT GGAAATGCTG GACCGTCGCA CTTATTGGTG CATATCTCGG 
CAGAGAGGCT GGGGTGTCCC CATACCTGTG TTTTACCATA AGGAGACAGG AAAGCCCCTC 
ATTAACCGGC ACAGCGTGGG GCATATTATG AGCCTGGTAG AGCAGCACGG GACGGATGTG 
TGGTGGACCC TTCCGCTAGA AGAATTGCTC CCCAAAGCCG TCCTTCAAAA GGTGGAAGGC 
AGCTCAGATG CGTGTGATTA TGAGCAAGGT CGCGATGTGT TGGACATCTG GTTTGACAGT 
GGAACGTCGT GGGCCCATGT TTTGGAAGAT GGAGAGCAGA GAGCGGATGT TTATATGGAA 
GGGAAGGACC AGCTGGGAGG TTGGTTTCAG TCATCGCTGC TCACCAGTGT GGCCGCCAGG 
AATAAGGCAC CGTATAGGAA TATCATCGTC CATGGCTTCA CCCTCAGTGA AAAAGGGGAG 
AAAATGTCTA AATCAGTAGG GAACGTAGTG GACCCCGATG TTGTTATTAA CGGAGGACAA 
GACAAGGTTA ATGAACCTGC TTATGGCGCC GATGTGCTGC GCTGGTGGGT GGCAGAGTCA 
AATGTCTTCA CTGAAGTGCT GATTGGTCCA GCTGTCCTTA ATTCTGCGCG GGATGATATC 
AACAAGTTGC GGAATACACT GCGCTTCATG CTGGGAAATC TATCGGATTT TACTCCAGAG 
AGGGACTCGG TCCCCAGCAC TGAGATGTAT GTGATAGACC AGTACATGCT TCATATCCTC 
CAAGATTACG GCAGCAAGGT TACAGACGCT TACAAGGGCT ATGAATTTGG GAAGGTGGTT 
CGTTTGCTGG AGGCCCTGGT CACACGGGAT TTATCAAGCT TTTACTTCAG CATCATAAAG 
GACAGGCTTT ATTGTGATGA AGAGAAGGAT CCCAAACGTC GTTCCTGCCA GACTGTATTA 
GCGGAAGCGC TGGATGTTGT GGTGCGCTCA TTCGCTCCCA TTCTACCCCA TTTGGCAGAG 
GAAGTGTTCC AGTACCTGCC CTATAGAAAA GAATCGGACA GCGCGTTCCG TACGGGATGG 
ATCAAACCCA ACACAGTCTG GAAGAAGCTG GGCCTGGTGG AAGCCATAGA GGCTGCCTGC 
TCCATCAGAG ATGCTTTCCT TGGATCCATT CCAGGCAAGA ATGCGGCTGA GTATGAAGTC 
ACCATCGTCA TTGATCCAGG CCTGCTGTTT GAGTTAATGG AGACCCTACA GGAAGAACCG 
AGCTCTTGTA CGTCTCAGCT GAATGAGCTC CTCATGTGCT CCCAGACCTC TCTCCTGTCC 
GACCTACCCC TGGATCTGCC CCCAGAAGCT TCTGTTTTGA AGGGCTCATT CCTTATCAAC 
TTGGAAGATG GCGACATCTG TCAGGAGGAG GCGTATAAAG TGGTGGTTCA GCCGAGCAGG 
AAGGAGCGCT GCCCGCGCTG CAGGAAGTAC ACGGCTGCCT CGTCCCTAGC GCCCTGTGCC 
AGGTGTCTGG AAGCCATCGC GGGCAAATGG ACTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120515.1
CDS	14..2992
Translation	MLRLLRCRLPVRRYRWGVRAASEKASSNESPGNRKYRGTVLLPHTDFPIRRGPGETASTELRLQEESGFLQLYAWQRERKTKKEFSLHDGPPYANGDPHVGHALNKILKDITNRFHMMRGYKVSYVPGWDCHGLPIELKALAELGEEAENMSPTEIRRKAKMFAEKAIEKQKSSFIRWGIMADWDKCYYTFDPKYEAKQLDIFYKMYDKGHIYQDYKPVFWSPSTRTALAEAELEYNQEHISRSIFAKFPMHKAPPKLASIIDGSSAVSTLVWTTQPWTIPANQAICYMPDTEYAIAKCANTGEQYILATDRIESTALSLGTQLQVISTFKGLDLEGGICLHPTIADRLSPLLPANHVTMAKGTGLVHTAPAHGIEDYSVASHHKLPVDCLVDESGHFTTGAGAELENKAVLTDGNETVIQMLHASKSLLKEESFVHSYPYDWRTKKPVIIRASKQWFVNTANIKDKAQDVLKKVKFVPASAAARMLEMLDRRTYWCISRQRGWGVPIPVFYHKETGKPLINRHSVGHIMSLVEQHGTDVWWTLPLEELLPKAVLQKVEGSSDACDYEQGRDVLDIWFDSGTSWAHVLEDGEQRADVYMEGKDQLGGWFQSSLLTSVAARNKAPYRNIIVHGFTLSEKGEKMSKSVGNVVDPDVVINGGQDKVNEPAYGADVLRWWVAESNVFTEVLIGPAVLNSARDDINKLRNTLRFMLGNLSDFTPERDSVPSTEMYVIDQYMLHILQDYGSKVTDAYKGYEFGKVVRLLEALVTRDLSSFYFSIIKDRLYCDEEKDPKRRSCQTVLAEALDVVVRSFAPILPHLAEEVFQYLPYRKESDSAFRTGWIKPNTVWKKLGLVEAIEAACSIRDAFLGSIPGKNAAEYEVTIVIDPGLLFELMETLQEEPSSCTSQLNELLMCSQTSLLSDLPLDLPPEASVLKGSFLINLEDGDICQEEAYKVVVQPSRKERCPRCRKYTAASSLAPCARCLEAIAGKWTQ

Target ORF information:

RefSeq Version	NM_001127043.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis isoleucyl-tRNA synthetase 2, mitochondrial (iars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127043.1

ORF Insert Sequence:

ATGCTGCGCC TTCTCCGGTG CCGGCTGCCA GTTCGGCGCT ACCGCTGGGG AGTAAGAGCC 
GCCTCTGAGA AGGCCAGCAG CAATGAGAGC CCCGGGAATA GGAAGTACCG GGGCACCGTG 
CTGCTCCCTC ACACTGACTT CCCTATCCGC CGGGGCCCGG GGGAGACAGC CAGCACCGAA 
CTACGGCTGC AGGAGGAAAG TGGATTTCTG CAACTCTATG CTTGGCAAAG GGAAAGGAAA 
ACCAAGAAGG AATTCAGTCT TCATGATGGA CCACCCTATG CCAATGGGGA CCCCCATGTG 
GGCCATGCTC TTAATAAGAT CCTCAAAGAT ATAACAAATC GTTTCCACAT GATGAGAGGC 
TACAAAGTGA GCTACGTGCC AGGATGGGAC TGCCATGGGC TGCCGATTGA ACTAAAAGCT 
CTGGCGGAAC TTGGAGAGGA AGCCGAAAAT ATGTCACCCA CTGAGATCAG GCGCAAAGCC 
AAGATGTTTG CAGAGAAAGC CATTGAAAAG CAGAAATCCT CCTTCATTCG CTGGGGTATC 
ATGGCAGACT GGGACAAGTG CTATTACACA TTCGATCCAA AGTATGAAGC AAAACAATTA 
GACATTTTCT ACAAGATGTA TGACAAGGGC CACATATACC AGGATTACAA GCCTGTCTTC 
TGGTCTCCAT CAACAAGAAC AGCCCTCGCA GAGGCAGAGT TGGAATACAA CCAGGAGCAC 
ATAAGTCGCT CAATATTTGC CAAATTCCCA ATGCACAAAG CTCCTCCAAA GCTGGCCTCC 
ATTATAGATG GATCCTCTGC AGTCAGCACT CTGGTTTGGA CGACCCAGCC TTGGACTATT 
CCTGCTAACC AGGCCATCTG CTATATGCCA GATACAGAGT ATGCCATAGC AAAGTGTGCT 
AATACTGGAG AACAGTACAT ATTAGCCACA GATAGAATTG AATCTACCGC TTTGTCCTTG 
GGTACCCAGC TTCAAGTCAT TTCAACCTTT AAAGGGCTAG ATCTGGAGGG CGGCATCTGT 
CTCCATCCCA CCATTGCTGA TCGCCTTTCT CCTCTCTTGC CTGCCAACCA CGTGACTATG 
GCAAAGGGCA CAGGCCTGGT ACACACTGCA CCGGCCCATG GCATTGAAGA CTACAGTGTT 
GCATCACATC ACAAGCTCCC AGTGGACTGT TTGGTGGATG AATCGGGGCA TTTTACTACT 
GGAGCTGGGG CTGAACTGGA AAATAAAGCT GTACTTACTG ATGGAAATGA GACAGTCATA 
CAGATGCTAC ATGCCAGCAA GTCTCTGCTG AAGGAGGAGA GTTTTGTACA CAGTTACCCC 
TATGACTGGA GAACAAAGAA ACCCGTGATA ATCCGTGCCA GTAAACAGTG GTTTGTCAAT 
ACAGCAAACA TTAAGGACAA AGCCCAGGAT GTGTTAAAGA AAGTGAAGTT TGTTCCCGCT 
TCAGCAGCTG CCAGAATGCT GGAAATGCTG GACCGTCGCA CTTATTGGTG CATATCTCGG 
CAGAGAGGCT GGGGTGTCCC CATACCTGTG TTTTACCATA AGGAGACAGG AAAGCCCCTC 
ATTAACCGGC ACAGCGTGGG GCATATTATG AGCCTGGTAG AGCAGCACGG GACGGATGTG 
TGGTGGACCC TTCCGCTAGA AGAATTGCTC CCCAAAGCCG TCCTTCAAAA GGTGGAAGGC 
AGCTCAGATG CGTGTGATTA TGAGCAAGGT CGCGATGTGT TGGACATCTG GTTTGACAGT 
GGAACGTCGT GGGCCCATGT TTTGGAAGAT GGAGAGCAGA GAGCGGATGT TTATATGGAA 
GGGAAGGACC AGCTGGGAGG TTGGTTTCAG TCATCGCTGC TCACCAGTGT GGCCGCCAGG 
AATAAGGCAC CGTATAGGAA TATCATCGTC CATGGCTTCA CCCTCAGTGA AAAAGGGGAG 
AAAATGTCTA AATCAGTAGG GAACGTAGTG GACCCCGATG TTGTTATTAA CGGAGGACAA 
GACAAGGTTA ATGAACCTGC TTATGGCGCC GATGTGCTGC GCTGGTGGGT GGCAGAGTCA 
AATGTCTTCA CTGAAGTGCT GATTGGTCCA GCTGTCCTTA ATTCTGCGCG GGATGATATC 
AACAAGTTGC GGAATACACT GCGCTTCATG CTGGGAAATC TATCGGATTT TACTCCAGAG 
AGGGACTCGG TCCCCAGCAC TGAGATGTAT GTGATAGACC AGTACATGCT TCATATCCTC 
CAAGATTACG GCAGCAAGGT TACAGACGCT TACAAGGGCT ATGAATTTGG GAAGGTGGTT 
CGTTTGCTGG AGGCCCTGGT CACACGGGAT TTATCAAGCT TTTACTTCAG CATCATAAAG 
GACAGGCTTT ATTGTGATGA AGAGAAGGAT CCCAAACGTC GTTCCTGCCA GACTGTATTA 
GCGGAAGCGC TGGATGTTGT GGTGCGCTCA TTCGCTCCCA TTCTACCCCA TTTGGCAGAG 
GAAGTGTTCC AGTACCTGCC CTATAGAAAA GAATCGGACA GCGCGTTCCG TACGGGATGG 
ATCAAACCCA ACACAGTCTG GAAGAAGCTG GGCCTGGTGG AAGCCATAGA GGCTGCCTGC 
TCCATCAGAG ATGCTTTCCT TGGATCCATT CCAGGCAAGA ATGCGGCTGA GTATGAAGTC 
ACCATCGTCA TTGATCCAGG CCTGCTGTTT GAGTTAATGG AGACCCTACA GGAAGAACCG 
AGCTCTTGTA CGTCTCAGCT GAATGAGCTC CTCATGTGCT CCCAGACCTC TCTCCTGTCC 
GACCTACCCC TGGATCTGCC CCCAGAAGCT TCTGTTTTGA AGGGCTCATT CCTTATCAAC 
TTGGAAGATG GCGACATCTG TCAGGAGGAG GCGTATAAAG TGGTGGTTCA GCCGAGCAGG 
AAGGAGCGCT GCCCGCGCTG CAGGAAGTAC ACGGCTGCCT CGTCCCTAGC GCCCTGTGCC 
AGGTGTCTGG AAGCCATCGC GGGCAAATGG ACTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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iars2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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