eprs1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	eprs1
Entrez Gene ID	100145057
Full Name	glutamyl-prolyl-tRNA synthetase
General protein information	Preferred Names glutamyl-prolyl-tRNA synthetase Names bifunctional glutamate/proline--tRNA ligase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002936017.3	XP_002936063.2	bifunctional glutamate/proline--tRNA ligase isoform X1
XM_031903114.1	XP_031758974.1	bifunctional glutamate/proline--tRNA ligase isoform X2
XM_002936018.3	XP_002936064.2	bifunctional glutamate/proline--tRNA ligase isoform X4
XM_031903113.1	XP_031758973.1	bifunctional glutamate/proline--tRNA ligase isoform X1
XM_012963956.1	XP_012819410.1	bifunctional glutamate/proline--tRNA ligase isoform X3
XM_012963955.1	XP_012819409.1	bifunctional glutamate/proline--tRNA ligase isoform X2
XM_031903115.1	XP_031758975.1	bifunctional glutamate/proline--tRNA ligase isoform X3

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	GUS1	NP_011269.2
Arabidopsis thaliana (thale cress)	AT5G26710	NP_850874.1
Xenopus tropicalis (tropical clawed frog)	eprs	XP_002936064.2
Pan troglodytes (chimpanzee)	EPRS	XP_001172425.1
	LOC706899	XP_002808297.1
Bos taurus (cattle)	EPRS	NP_001230249.1
Danio rerio (zebrafish)	eprs	NP_001191177.1
Canis lupus familiaris (dog)	EPRS	XP_005640882.1
Magnaporthe oryzae (rice blast fungus)	MGG_05956	XP_003711794.1
Neurospora crassa	NCU08894	XP_958832.1
Kluyveromyces lactis	KLLA0A00682g	XP_451028.1
Eremothecium gossypii	AGOS_AFR264W	NP_985811.2
Schizosaccharomyces pombe (fission yeast)	SPAC17A5.15c	NP_593483.1
Oryza sativa (rice)	Os10g0369000	NP_001064451.1
Homo sapiens (human)	EPRS	NP_004437.2
Mus musculus (house mouse)	Eprs	NP_084011.1
Rattus norvegicus (Norway rat)	Eprs	NP_001019409.1
Oryza sativa (rice)	Os01g0271200	NP_001042706.1

Gene Ontology

The following eprs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eprs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa59435	XM_031903113.1 Latest version!	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase 1 (eprs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa59436	XM_031903114.1 Latest version!	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase 1 (eprs1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa59437	XM_031903115.1 Latest version!	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase 1 (eprs1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa10927	XM_002936017.3 Latest version!	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa10928	XM_002936018.3 Latest version!	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa31526	XM_012963955.1 Latest version!	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa31527	XM_012963956.1 Latest version!	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eprs1 ( XM_031903113.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758973.1 Xenopus tropicalis glutamyl-prolyl-tRNA synthetase 1 (eprs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa59435
Clone ID Related Accession (Same CDS sequence)	XM_031903113.1
Accession Version	XM_031903113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bifunctional glutamate/proline--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACTA GCCCTTCCAC ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATCAGGTAG ATGGAGCTGT GAAAACCCTT 
CTAGCTTTAA AAGCAGAATA TAAACAAGTA ACAGGTCAGG AGTACAAGCC AGGGAGCCCT 
CCAGTAGTGC CAAGCAGCTC TTCTCCACCT CCCGCTAGTC AGAGCTTCTC CAATACCTCA 
GATCTGATAT TGTACAACAG AGTGGTTGAG CAAGGAGAGG TGGTCCGACA GCTCAAAAGC 
AAGAAAGCCC CCAAGGATGA GGTAGATGCA GCCGTGAAAC TTCTTTTGAA ACTTAAAGCT 
GAATACAAGC AATTGACTGG CCAGGAATAT AAGCCTCAGA CTCCACCTTT ATCTCAGCCA 
TCAAGTGAAC CCTCAAAAGT TTTGTCTGGT TCAGAGCCAG CGGCCCTCAG TTCTACTGAC 
TGTGCAAGTC TGTATGAGAA AGTAGCTGAG CAAGGCGATC TGGTCCGCAA GCTTAAATCT 
GAGAAAGCGC CAAAGGATAA AGTGGATGAG GCTGTGAAAC TGCTACTGGC TCTGAAGGCT 
GAATATAAGG AGAAGACAGG GCAGGAGTAT AAACCAGGAC AGACCCCGGC TGCTGCTCCC 
ACAAAAAAGG CCCTGGAAAA ACCCACAAGT TCTCAGGCAA ATGGCTCACA CAGCGCCGAG 
GCAGAAGAGC TGTATAACAG AGTGGCCAGA CAGGGGGAGG AAGTACGCAA AGTGAAGTCT 
GAGAAAGCCG CAAAGGACAA AGTGGATGCT GCCGTAAAGG AGCTTCTAGA TTTAAAGGCT 
CAATACAAAG CTTTGACGGG CACAGAGTAC AAACCAGTGG CTGCCCCAAG CTCAGAGGAA 
AAAAAGCACA AGGAGAAGGA GAACAAGTCT GAGAAGCAAA GCAAGCAATC AAAGGCTGGA 
GAAGGGCCCA AGAAGAAGGA TACTTCCAAA GATCAAGGGG ACAATTCTGC TGCTGGAGAA 
AGTCAAGGAC CTAAAAAACA GACCAGGTTG GGATTGGAGG CTAAAAAGGA AGAGAACTTA 
CCAGACTGGT ATTCCCAGGT GATCACCAAA TCAGAGATGA TCGAATACTA TGACGTCAGT 
GGCTGTTACG TGCTGCGTCC CTGGGCCTTT GCCATCTGGG AGGCCATTAA AGAGTTCTTT 
GATAAAGAGA TAAAGAAGCT CGGTGTGGAA AACTGTTACT TCCCCATGTT CGTGTCTCAG 
GCTGCGCTGG AGAAGGAAAA AACCCACATT GCAGATTTCG CTCCAGAAGT TGCCTGGGTT 
ACAAGGTCAG GAAAGACAGA GCTAGCAGAA CCAATTGCTG TACGGCCCAC AAGTGAAACT 
GTGATGTATC CCGCTTATGC CAAATGGGTG CAGTCCCACC GAGATCTCCC CATTAAGCTG 
AATCAGTGGT GTAATGTAGT GAGGTGGGAA TTTAAACACC CTCAGCCTTT CCTCCGAACA 
CGTGAGTTCC TTTGGCAAGA AGGACATACA GCGTTCGCTA CATACGAGGA AGCTGCAGAA 
GAGGTCTTGC AGATACTGGA TCTTTATGCC CGTGTCTATG AGGATCTCTT GGCTATTCCT 
GTTGTAAAAG GAAGAAAGAC TGAAAAAGAA AAGTTTGCAG GTGGGGACTA CACAACCACA 
GTGGAAGCAT TTATATCGGC CAGCAGCCGT GCCATACAGG GTGCAACATC ACATCACCTT 
GGCCAGAACT TTTCCAAAAT GTTTGAAATT GTCTTTGAAG ACCCTAAAAC TCCTGGGTTA 
AAACAGTATG CGTACCAGAA CTCATGGGGT CTTTCAACTC GCACAATAGG AGCAATGACC 
ATGGTTCATG CAGACAACAT TGGGATGGTG CTCCCACCCA GAGTAGCCTG TGTGCAGGTA 
ATAATAATCC CTTGTGGCAT CACAAACACC CTTTCCGAGG AAGACAAAGA TGCCCTCCTA 
AAGAAATGTG ACCAGTATCT GAAGCGCCTT CTCAACGTTG ATGTACGGGC ACGCGCAGAT 
GTGAGGGACA ACTACTCCCC GGGATGGAAG TTCAATCACT GGGAGCTCAA GGGTGTTCCA 
ATCAGGCTTG AGGTTGGGCC ACGAGACATG AAAAATAAGC AGTTTGTAGC AGTCAGAAGA 
GACAATGGAG AGAAACAAAC CATCTCAGAG GACCAGGCCG AGACGCGGTT AAAGGAAATC 
TTGGAGGAAA TTCACACAAA TCTATATAAC AGGGCGCTCA GTGATCTGAC AAGTAACATG 
GTGGTAGCCA ACACGATGGA AGACTTCCAG AAGAATTTGG AGAAGGGAAG GATTGTACAG 
ATCCCATTCT GCGGGGAAAT TGAATGTGAA GATTGGATAA AGAAGGTAAC AGCAAGGGAT 
CAAGATCTGG AGCCAGGAGC ACCTTCCATG GGAGCCAAAA GCCTCTGTAT CCCCTTCAAA 
CCTCTCAGTG AGCTGCAGCC CAGCGCCAAG TGTGTCTGCG AGAAGAACGC CGCCAAGTTC 
TACACACTAT TTGGCCGCAG TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758973.1
CDS	42..5168
Translation	MSTSPSTQEVRLLPGQCWSVCEPEAGCTLSHSARSFVAAAGISSSIAGGDSMALTLTVSSSSPSLAVLWTVEHLKGQIQIHVQEGKDTHLRVTDTISFTDVHSIVRYLARLAPDKGLSGSHLTEQTEVSHWMEFSQTKLMQKGEFSTGLQELNHCLSLRTYLVGNALTLADICVFSALEASSEWQALVQKGQTPVNVRRWYSFLEPQLGFWSRTNNKVPSEKKQDVGKFVELPGAEMGKVIVRFPPEASGYLHIGHAKAALLNQHYQVTFKGKLIMRFDDTNPEKEKEDFEKVILEDVSMLQIKPDQFTYTSDHFDTIMKYAEKLIQEGKAYVDDTPAEQMKSEREQRIESKHRSNTVAKNMEMWEDMKKGTEYGQTCCLRAKIDMNSNNGCMRDPTLFRCKNQPHPRTGSKYNIYPTYDFACPIVDSVEGVTHALRTTEYHDRDEQFYWIIDALGIRKPYIWEYSRLNLNNTVLSKRRLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYSALLKSQAVAVNIPDAKEGMVEVAKHPKNAEVGQKPVWYGPKVLIEGADAETLSEGETVTFINWGNLIITKIHRDQSGKIQSLDAKLNLENKDYKKTTKITWLAETPKAPFIPTSCVNYDHLITKPVLGKEEDFKQYVNRSSKQEEVMLGDPCLKDLKKGDLIQLQRRGFYICDEPYEPISPHSCKEAPCVLIYIPDGHTKEMPTSGSKEKSKTEATKKETQPKSKGKAANVPVSVAPLASPEEDPVALYNKVSAQGDLVRDLKSKKAAKAEVDEAVKLLLSLKAQYKEVTGQEYKPGSAPAQKPAPSPAASSSGDAKAIYEKVSAQGETVRKLKTEKAPKDQVDGAVKTLLALKAEYKQVTGQEYKPGSPPVVPSSSSPPPASQSFSNTSDLILYNRVVEQGEVVRQLKSKKAPKDEVDAAVKLLLKLKAEYKQLTGQEYKPQTPPLSQPSSEPSKVLSGSEPAALSSTDCASLYEKVAEQGDLVRKLKSEKAPKDKVDEAVKLLLALKAEYKEKTGQEYKPGQTPAAAPTKKALEKPTSSQANGSHSAEAEELYNRVARQGEEVRKVKSEKAAKDKVDAAVKELLDLKAQYKALTGTEYKPVAAPSSEEKKHKEKENKSEKQSKQSKAGEGPKKKDTSKDQGDNSAAGESQGPKKQTRLGLEAKKEENLPDWYSQVITKSEMIEYYDVSGCYVLRPWAFAIWEAIKEFFDKEIKKLGVENCYFPMFVSQAALEKEKTHIADFAPEVAWVTRSGKTELAEPIAVRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHTAFATYEEAAEEVLQILDLYARVYEDLLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASSRAIQGATSHHLGQNFSKMFEIVFEDPKTPGLKQYAYQNSWGLSTRTIGAMTMVHADNIGMVLPPRVACVQVIIIPCGITNTLSEEDKDALLKKCDQYLKRLLNVDVRARADVRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNKQFVAVRRDNGEKQTISEDQAETRLKEILEEIHTNLYNRALSDLTSNMVVANTMEDFQKNLEKGRIVQIPFCGEIECEDWIKKVTARDQDLEPGAPSMGAKSLCIPFKPLSELQPSAKCVCEKNAAKFYTLFGRSY

Target ORF information:

RefSeq Version	XM_031903113.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase 1 (eprs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903113.1

ORF Insert Sequence:

ATGTCTACTA GCCCTTCCAC ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATCAGGTAG ATGGAGCTGT GAAAACCCTT 
CTAGCTTTAA AAGCAGAATA TAAACAAGTA ACAGGTCAGG AGTACAAGCC AGGGAGCCCT 
CCAGTAGTGC CAAGCAGCTC TTCTCCACCT CCCGCTAGTC AGAGCTTCTC CAATACCTCA 
GATCTGATAT TGTACAACAG AGTGGTTGAG CAAGGAGAGG TGGTCCGACA GCTCAAAAGC 
AAGAAAGCCC CCAAGGATGA GGTAGATGCA GCCGTGAAAC TTCTTTTGAA ACTTAAAGCT 
GAATACAAGC AATTGACTGG CCAGGAATAT AAGCCTCAGA CTCCACCTTT ATCTCAGCCA 
TCAAGTGAAC CCTCAAAAGT TTTGTCTGGT TCAGAGCCAG CGGCCCTCAG TTCTACTGAC 
TGTGCAAGTC TGTATGAGAA AGTAGCTGAG CAAGGCGATC TGGTCCGCAA GCTTAAATCT 
GAGAAAGCGC CAAAGGATAA AGTGGATGAG GCTGTGAAAC TGCTACTGGC TCTGAAGGCT 
GAATATAAGG AGAAGACAGG GCAGGAGTAT AAACCAGGAC AGACCCCGGC TGCTGCTCCC 
ACAAAAAAGG CCCTGGAAAA ACCCACAAGT TCTCAGGCAA ATGGCTCACA CAGCGCCGAG 
GCAGAAGAGC TGTATAACAG AGTGGCCAGA CAGGGGGAGG AAGTACGCAA AGTGAAGTCT 
GAGAAAGCCG CAAAGGACAA AGTGGATGCT GCCGTAAAGG AGCTTCTAGA TTTAAAGGCT 
CAATACAAAG CTTTGACGGG CACAGAGTAC AAACCAGTGG CTGCCCCAAG CTCAGAGGAA 
AAAAAGCACA AGGAGAAGGA GAACAAGTCT GAGAAGCAAA GCAAGCAATC AAAGGCTGGA 
GAAGGGCCCA AGAAGAAGGA TACTTCCAAA GATCAAGGGG ACAATTCTGC TGCTGGAGAA 
AGTCAAGGAC CTAAAAAACA GACCAGGTTG GGATTGGAGG CTAAAAAGGA AGAGAACTTA 
CCAGACTGGT ATTCCCAGGT GATCACCAAA TCAGAGATGA TCGAATACTA TGACGTCAGT 
GGCTGTTACG TGCTGCGTCC CTGGGCCTTT GCCATCTGGG AGGCCATTAA AGAGTTCTTT 
GATAAAGAGA TAAAGAAGCT CGGTGTGGAA AACTGTTACT TCCCCATGTT CGTGTCTCAG 
GCTGCGCTGG AGAAGGAAAA AACCCACATT GCAGATTTCG CTCCAGAAGT TGCCTGGGTT 
ACAAGGTCAG GAAAGACAGA GCTAGCAGAA CCAATTGCTG TACGGCCCAC AAGTGAAACT 
GTGATGTATC CCGCTTATGC CAAATGGGTG CAGTCCCACC GAGATCTCCC CATTAAGCTG 
AATCAGTGGT GTAATGTAGT GAGGTGGGAA TTTAAACACC CTCAGCCTTT CCTCCGAACA 
CGTGAGTTCC TTTGGCAAGA AGGACATACA GCGTTCGCTA CATACGAGGA AGCTGCAGAA 
GAGGTCTTGC AGATACTGGA TCTTTATGCC CGTGTCTATG AGGATCTCTT GGCTATTCCT 
GTTGTAAAAG GAAGAAAGAC TGAAAAAGAA AAGTTTGCAG GTGGGGACTA CACAACCACA 
GTGGAAGCAT TTATATCGGC CAGCAGCCGT GCCATACAGG GTGCAACATC ACATCACCTT 
GGCCAGAACT TTTCCAAAAT GTTTGAAATT GTCTTTGAAG ACCCTAAAAC TCCTGGGTTA 
AAACAGTATG CGTACCAGAA CTCATGGGGT CTTTCAACTC GCACAATAGG AGCAATGACC 
ATGGTTCATG CAGACAACAT TGGGATGGTG CTCCCACCCA GAGTAGCCTG TGTGCAGGTA 
ATAATAATCC CTTGTGGCAT CACAAACACC CTTTCCGAGG AAGACAAAGA TGCCCTCCTA 
AAGAAATGTG ACCAGTATCT GAAGCGCCTT CTCAACGTTG ATGTACGGGC ACGCGCAGAT 
GTGAGGGACA ACTACTCCCC GGGATGGAAG TTCAATCACT GGGAGCTCAA GGGTGTTCCA 
ATCAGGCTTG AGGTTGGGCC ACGAGACATG AAAAATAAGC AGTTTGTAGC AGTCAGAAGA 
GACAATGGAG AGAAACAAAC CATCTCAGAG GACCAGGCCG AGACGCGGTT AAAGGAAATC 
TTGGAGGAAA TTCACACAAA TCTATATAAC AGGGCGCTCA GTGATCTGAC AAGTAACATG 
GTGGTAGCCA ACACGATGGA AGACTTCCAG AAGAATTTGG AGAAGGGAAG GATTGTACAG 
ATCCCATTCT GCGGGGAAAT TGAATGTGAA GATTGGATAA AGAAGGTAAC AGCAAGGGAT 
CAAGATCTGG AGCCAGGAGC ACCTTCCATG GGAGCCAAAA GCCTCTGTAT CCCCTTCAAA 
CCTCTCAGTG AGCTGCAGCC CAGCGCCAAG TGTGTCTGCG AGAAGAACGC CGCCAAGTTC 
TACACACTAT TTGGCCGCAG TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eprs1 ( XM_031903114.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758974.1 Xenopus tropicalis glutamyl-prolyl-tRNA synthetase 1 (eprs1), transcript variant X2, mRNA.

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CloneID	OXa59436
Clone ID Related Accession (Same CDS sequence)	XM_031903114.1
Accession Version	XM_031903114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bifunctional glutamate/proline--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACTA GCCCTTCCAC ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATCAGGTAG ATGGAGCTGT GAAAACCCTT 
CTAGCTTTAA AAGCAGAATA TAAACAAGTA ACAGGTCAGG AGTACAAGCC AGGGAGCCCT 
CCAGTAGTGC CAAGCAGCTC TTCTCCACCT CCCGCTAGTC AGAGCTTCTC CAATACCTCA 
GATCTGATAT TGTACAACAG AGTGGTTGAG CAAGGAGAGG TGGTCCGACA GCTCAAAAGC 
AAGAAAGCCC CCAAGGATAA AGTGGATGAG GCTGTGAAAC TGCTACTGGC TCTGAAGGCT 
GAATATAAGG AGAAGACAGG GCAGGAGTAT AAACCAGGAC AGACCCCGGC TGCTGCTCCC 
ACAAAAAAGG CCCTGGAAAA ACCCACAAGT TCTCAGGCAA ATGGCTCACA CAGCGCCGAG 
GCAGAAGAGC TGTATAACAG AGTGGCCAGA CAGGGGGAGG AAGTACGCAA AGTGAAGTCT 
GAGAAAGCCG CAAAGGACAA AGTGGATGCT GCCGTAAAGG AGCTTCTAGA TTTAAAGGCT 
CAATACAAAG CTTTGACGGG CACAGAGTAC AAACCAGTGG CTGCCCCAAG CTCAGAGGAA 
AAAAAGCACA AGGAGAAGGA GAACAAGTCT GAGAAGCAAA GCAAGCAATC AAAGGCTGGA 
GAAGGGCCCA AGAAGAAGGA TACTTCCAAA GATCAAGGGG ACAATTCTGC TGCTGGAGAA 
AGTCAAGGAC CTAAAAAACA GACCAGGTTG GGATTGGAGG CTAAAAAGGA AGAGAACTTA 
CCAGACTGGT ATTCCCAGGT GATCACCAAA TCAGAGATGA TCGAATACTA TGACGTCAGT 
GGCTGTTACG TGCTGCGTCC CTGGGCCTTT GCCATCTGGG AGGCCATTAA AGAGTTCTTT 
GATAAAGAGA TAAAGAAGCT CGGTGTGGAA AACTGTTACT TCCCCATGTT CGTGTCTCAG 
GCTGCGCTGG AGAAGGAAAA AACCCACATT GCAGATTTCG CTCCAGAAGT TGCCTGGGTT 
ACAAGGTCAG GAAAGACAGA GCTAGCAGAA CCAATTGCTG TACGGCCCAC AAGTGAAACT 
GTGATGTATC CCGCTTATGC CAAATGGGTG CAGTCCCACC GAGATCTCCC CATTAAGCTG 
AATCAGTGGT GTAATGTAGT GAGGTGGGAA TTTAAACACC CTCAGCCTTT CCTCCGAACA 
CGTGAGTTCC TTTGGCAAGA AGGACATACA GCGTTCGCTA CATACGAGGA AGCTGCAGAA 
GAGGTCTTGC AGATACTGGA TCTTTATGCC CGTGTCTATG AGGATCTCTT GGCTATTCCT 
GTTGTAAAAG GAAGAAAGAC TGAAAAAGAA AAGTTTGCAG GTGGGGACTA CACAACCACA 
GTGGAAGCAT TTATATCGGC CAGCAGCCGT GCCATACAGG GTGCAACATC ACATCACCTT 
GGCCAGAACT TTTCCAAAAT GTTTGAAATT GTCTTTGAAG ACCCTAAAAC TCCTGGGTTA 
AAACAGTATG CGTACCAGAA CTCATGGGGT CTTTCAACTC GCACAATAGG AGCAATGACC 
ATGGTTCATG CAGACAACAT TGGGATGGTG CTCCCACCCA GAGTAGCCTG TGTGCAGGTA 
ATAATAATCC CTTGTGGCAT CACAAACACC CTTTCCGAGG AAGACAAAGA TGCCCTCCTA 
AAGAAATGTG ACCAGTATCT GAAGCGCCTT CTCAACGTTG ATGTACGGGC ACGCGCAGAT 
GTGAGGGACA ACTACTCCCC GGGATGGAAG TTCAATCACT GGGAGCTCAA GGGTGTTCCA 
ATCAGGCTTG AGGTTGGGCC ACGAGACATG AAAAATAAGC AGTTTGTAGC AGTCAGAAGA 
GACAATGGAG AGAAACAAAC CATCTCAGAG GACCAGGCCG AGACGCGGTT AAAGGAAATC 
TTGGAGGAAA TTCACACAAA TCTATATAAC AGGGCGCTCA GTGATCTGAC AAGTAACATG 
GTGGTAGCCA ACACGATGGA AGACTTCCAG AAGAATTTGG AGAAGGGAAG GATTGTACAG 
ATCCCATTCT GCGGGGAAAT TGAATGTGAA GATTGGATAA AGAAGGTAAC AGCAAGGGAT 
CAAGATCTGG AGCCAGGAGC ACCTTCCATG GGAGCCAAAA GCCTCTGTAT CCCCTTCAAA 
CCTCTCAGTG AGCTGCAGCC CAGCGCCAAG TGTGTCTGCG AGAAGAACGC CGCCAAGTTC 
TACACACTAT TTGGCCGCAG TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758974.1
CDS	42..4928
Translation	MSTSPSTQEVRLLPGQCWSVCEPEAGCTLSHSARSFVAAAGISSSIAGGDSMALTLTVSSSSPSLAVLWTVEHLKGQIQIHVQEGKDTHLRVTDTISFTDVHSIVRYLARLAPDKGLSGSHLTEQTEVSHWMEFSQTKLMQKGEFSTGLQELNHCLSLRTYLVGNALTLADICVFSALEASSEWQALVQKGQTPVNVRRWYSFLEPQLGFWSRTNNKVPSEKKQDVGKFVELPGAEMGKVIVRFPPEASGYLHIGHAKAALLNQHYQVTFKGKLIMRFDDTNPEKEKEDFEKVILEDVSMLQIKPDQFTYTSDHFDTIMKYAEKLIQEGKAYVDDTPAEQMKSEREQRIESKHRSNTVAKNMEMWEDMKKGTEYGQTCCLRAKIDMNSNNGCMRDPTLFRCKNQPHPRTGSKYNIYPTYDFACPIVDSVEGVTHALRTTEYHDRDEQFYWIIDALGIRKPYIWEYSRLNLNNTVLSKRRLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYSALLKSQAVAVNIPDAKEGMVEVAKHPKNAEVGQKPVWYGPKVLIEGADAETLSEGETVTFINWGNLIITKIHRDQSGKIQSLDAKLNLENKDYKKTTKITWLAETPKAPFIPTSCVNYDHLITKPVLGKEEDFKQYVNRSSKQEEVMLGDPCLKDLKKGDLIQLQRRGFYICDEPYEPISPHSCKEAPCVLIYIPDGHTKEMPTSGSKEKSKTEATKKETQPKSKGKAANVPVSVAPLASPEEDPVALYNKVSAQGDLVRDLKSKKAAKAEVDEAVKLLLSLKAQYKEVTGQEYKPGSAPAQKPAPSPAASSSGDAKAIYEKVSAQGETVRKLKTEKAPKDQVDGAVKTLLALKAEYKQVTGQEYKPGSPPVVPSSSSPPPASQSFSNTSDLILYNRVVEQGEVVRQLKSKKAPKDKVDEAVKLLLALKAEYKEKTGQEYKPGQTPAAAPTKKALEKPTSSQANGSHSAEAEELYNRVARQGEEVRKVKSEKAAKDKVDAAVKELLDLKAQYKALTGTEYKPVAAPSSEEKKHKEKENKSEKQSKQSKAGEGPKKKDTSKDQGDNSAAGESQGPKKQTRLGLEAKKEENLPDWYSQVITKSEMIEYYDVSGCYVLRPWAFAIWEAIKEFFDKEIKKLGVENCYFPMFVSQAALEKEKTHIADFAPEVAWVTRSGKTELAEPIAVRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHTAFATYEEAAEEVLQILDLYARVYEDLLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASSRAIQGATSHHLGQNFSKMFEIVFEDPKTPGLKQYAYQNSWGLSTRTIGAMTMVHADNIGMVLPPRVACVQVIIIPCGITNTLSEEDKDALLKKCDQYLKRLLNVDVRARADVRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNKQFVAVRRDNGEKQTISEDQAETRLKEILEEIHTNLYNRALSDLTSNMVVANTMEDFQKNLEKGRIVQIPFCGEIECEDWIKKVTARDQDLEPGAPSMGAKSLCIPFKPLSELQPSAKCVCEKNAAKFYTLFGRSY

Target ORF information:

RefSeq Version	XM_031903114.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase 1 (eprs1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903114.1

ORF Insert Sequence:

ATGTCTACTA GCCCTTCCAC ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATCAGGTAG ATGGAGCTGT GAAAACCCTT 
CTAGCTTTAA AAGCAGAATA TAAACAAGTA ACAGGTCAGG AGTACAAGCC AGGGAGCCCT 
CCAGTAGTGC CAAGCAGCTC TTCTCCACCT CCCGCTAGTC AGAGCTTCTC CAATACCTCA 
GATCTGATAT TGTACAACAG AGTGGTTGAG CAAGGAGAGG TGGTCCGACA GCTCAAAAGC 
AAGAAAGCCC CCAAGGATAA AGTGGATGAG GCTGTGAAAC TGCTACTGGC TCTGAAGGCT 
GAATATAAGG AGAAGACAGG GCAGGAGTAT AAACCAGGAC AGACCCCGGC TGCTGCTCCC 
ACAAAAAAGG CCCTGGAAAA ACCCACAAGT TCTCAGGCAA ATGGCTCACA CAGCGCCGAG 
GCAGAAGAGC TGTATAACAG AGTGGCCAGA CAGGGGGAGG AAGTACGCAA AGTGAAGTCT 
GAGAAAGCCG CAAAGGACAA AGTGGATGCT GCCGTAAAGG AGCTTCTAGA TTTAAAGGCT 
CAATACAAAG CTTTGACGGG CACAGAGTAC AAACCAGTGG CTGCCCCAAG CTCAGAGGAA 
AAAAAGCACA AGGAGAAGGA GAACAAGTCT GAGAAGCAAA GCAAGCAATC AAAGGCTGGA 
GAAGGGCCCA AGAAGAAGGA TACTTCCAAA GATCAAGGGG ACAATTCTGC TGCTGGAGAA 
AGTCAAGGAC CTAAAAAACA GACCAGGTTG GGATTGGAGG CTAAAAAGGA AGAGAACTTA 
CCAGACTGGT ATTCCCAGGT GATCACCAAA TCAGAGATGA TCGAATACTA TGACGTCAGT 
GGCTGTTACG TGCTGCGTCC CTGGGCCTTT GCCATCTGGG AGGCCATTAA AGAGTTCTTT 
GATAAAGAGA TAAAGAAGCT CGGTGTGGAA AACTGTTACT TCCCCATGTT CGTGTCTCAG 
GCTGCGCTGG AGAAGGAAAA AACCCACATT GCAGATTTCG CTCCAGAAGT TGCCTGGGTT 
ACAAGGTCAG GAAAGACAGA GCTAGCAGAA CCAATTGCTG TACGGCCCAC AAGTGAAACT 
GTGATGTATC CCGCTTATGC CAAATGGGTG CAGTCCCACC GAGATCTCCC CATTAAGCTG 
AATCAGTGGT GTAATGTAGT GAGGTGGGAA TTTAAACACC CTCAGCCTTT CCTCCGAACA 
CGTGAGTTCC TTTGGCAAGA AGGACATACA GCGTTCGCTA CATACGAGGA AGCTGCAGAA 
GAGGTCTTGC AGATACTGGA TCTTTATGCC CGTGTCTATG AGGATCTCTT GGCTATTCCT 
GTTGTAAAAG GAAGAAAGAC TGAAAAAGAA AAGTTTGCAG GTGGGGACTA CACAACCACA 
GTGGAAGCAT TTATATCGGC CAGCAGCCGT GCCATACAGG GTGCAACATC ACATCACCTT 
GGCCAGAACT TTTCCAAAAT GTTTGAAATT GTCTTTGAAG ACCCTAAAAC TCCTGGGTTA 
AAACAGTATG CGTACCAGAA CTCATGGGGT CTTTCAACTC GCACAATAGG AGCAATGACC 
ATGGTTCATG CAGACAACAT TGGGATGGTG CTCCCACCCA GAGTAGCCTG TGTGCAGGTA 
ATAATAATCC CTTGTGGCAT CACAAACACC CTTTCCGAGG AAGACAAAGA TGCCCTCCTA 
AAGAAATGTG ACCAGTATCT GAAGCGCCTT CTCAACGTTG ATGTACGGGC ACGCGCAGAT 
GTGAGGGACA ACTACTCCCC GGGATGGAAG TTCAATCACT GGGAGCTCAA GGGTGTTCCA 
ATCAGGCTTG AGGTTGGGCC ACGAGACATG AAAAATAAGC AGTTTGTAGC AGTCAGAAGA 
GACAATGGAG AGAAACAAAC CATCTCAGAG GACCAGGCCG AGACGCGGTT AAAGGAAATC 
TTGGAGGAAA TTCACACAAA TCTATATAAC AGGGCGCTCA GTGATCTGAC AAGTAACATG 
GTGGTAGCCA ACACGATGGA AGACTTCCAG AAGAATTTGG AGAAGGGAAG GATTGTACAG 
ATCCCATTCT GCGGGGAAAT TGAATGTGAA GATTGGATAA AGAAGGTAAC AGCAAGGGAT 
CAAGATCTGG AGCCAGGAGC ACCTTCCATG GGAGCCAAAA GCCTCTGTAT CCCCTTCAAA 
CCTCTCAGTG AGCTGCAGCC CAGCGCCAAG TGTGTCTGCG AGAAGAACGC CGCCAAGTTC 
TACACACTAT TTGGCCGCAG TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eprs1 ( XM_031903115.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758975.1 Xenopus tropicalis glutamyl-prolyl-tRNA synthetase 1 (eprs1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa59437
Clone ID Related Accession (Same CDS sequence)	XM_031903115.1
Accession Version	XM_031903115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bifunctional glutamate/proline--tRNA ligase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACTA GCCCTTCCAC ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATAAAGTGG ATGAGGCTGT GAAACTGCTA 
CTGGCTCTGA AGGCTGAATA TAAGGAGAAG ACAGGGCAGG AGTATAAACC AGGACAGACC 
CCGGCTGCTG CTCCCACAAA AAAGGCCCTG GAAAAACCCA CAAGTTCTCA GGCAAATGGC 
TCACACAGCG CCGAGGCAGA AGAGCTGTAT AACAGAGTGG CCAGACAGGG GGAGGAAGTA 
CGCAAAGTGA AGTCTGAGAA AGCCGCAAAG GACAAAGTGG ATGCTGCCGT AAAGGAGCTT 
CTAGATTTAA AGGCTCAATA CAAAGCTTTG ACGGGCACAG AGTACAAACC AGTGGCTGCC 
CCAAGCTCAG AGGAAAAAAA GCACAAGGAG AAGGAGAACA AGTCTGAGAA GCAAAGCAAG 
CAATCAAAGG CTGGAGAAGG GCCCAAGAAG AAGGATACTT CCAAAGATCA AGGGGACAAT 
TCTGCTGCTG GAGAAAGTCA AGGACCTAAA AAACAGACCA GGTTGGGATT GGAGGCTAAA 
AAGGAAGAGA ACTTACCAGA CTGGTATTCC CAGGTGATCA CCAAATCAGA GATGATCGAA 
TACTATGACG TCAGTGGCTG TTACGTGCTG CGTCCCTGGG CCTTTGCCAT CTGGGAGGCC 
ATTAAAGAGT TCTTTGATAA AGAGATAAAG AAGCTCGGTG TGGAAAACTG TTACTTCCCC 
ATGTTCGTGT CTCAGGCTGC GCTGGAGAAG GAAAAAACCC ACATTGCAGA TTTCGCTCCA 
GAAGTTGCCT GGGTTACAAG GTCAGGAAAG ACAGAGCTAG CAGAACCAAT TGCTGTACGG 
CCCACAAGTG AAACTGTGAT GTATCCCGCT TATGCCAAAT GGGTGCAGTC CCACCGAGAT 
CTCCCCATTA AGCTGAATCA GTGGTGTAAT GTAGTGAGGT GGGAATTTAA ACACCCTCAG 
CCTTTCCTCC GAACACGTGA GTTCCTTTGG CAAGAAGGAC ATACAGCGTT CGCTACATAC 
GAGGAAGCTG CAGAAGAGGT CTTGCAGATA CTGGATCTTT ATGCCCGTGT CTATGAGGAT 
CTCTTGGCTA TTCCTGTTGT AAAAGGAAGA AAGACTGAAA AAGAAAAGTT TGCAGGTGGG 
GACTACACAA CCACAGTGGA AGCATTTATA TCGGCCAGCA GCCGTGCCAT ACAGGGTGCA 
ACATCACATC ACCTTGGCCA GAACTTTTCC AAAATGTTTG AAATTGTCTT TGAAGACCCT 
AAAACTCCTG GGTTAAAACA GTATGCGTAC CAGAACTCAT GGGGTCTTTC AACTCGCACA 
ATAGGAGCAA TGACCATGGT TCATGCAGAC AACATTGGGA TGGTGCTCCC ACCCAGAGTA 
GCCTGTGTGC AGGTAATAAT AATCCCTTGT GGCATCACAA ACACCCTTTC CGAGGAAGAC 
AAAGATGCCC TCCTAAAGAA ATGTGACCAG TATCTGAAGC GCCTTCTCAA CGTTGATGTA 
CGGGCACGCG CAGATGTGAG GGACAACTAC TCCCCGGGAT GGAAGTTCAA TCACTGGGAG 
CTCAAGGGTG TTCCAATCAG GCTTGAGGTT GGGCCACGAG ACATGAAAAA TAAGCAGTTT 
GTAGCAGTCA GAAGAGACAA TGGAGAGAAA CAAACCATCT CAGAGGACCA GGCCGAGACG 
CGGTTAAAGG AAATCTTGGA GGAAATTCAC ACAAATCTAT ATAACAGGGC GCTCAGTGAT 
CTGACAAGTA ACATGGTGGT AGCCAACACG ATGGAAGACT TCCAGAAGAA TTTGGAGAAG 
GGAAGGATTG TACAGATCCC ATTCTGCGGG GAAATTGAAT GTGAAGATTG GATAAAGAAG 
GTAACAGCAA GGGATCAAGA TCTGGAGCCA GGAGCACCTT CCATGGGAGC CAAAAGCCTC 
TGTATCCCCT TCAAACCTCT CAGTGAGCTG CAGCCCAGCG CCAAGTGTGT CTGCGAGAAG 
AACGCCGCCA AGTTCTACAC ACTATTTGGC CGCAGTTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758975.1
CDS	42..4703
Translation	MSTSPSTQEVRLLPGQCWSVCEPEAGCTLSHSARSFVAAAGISSSIAGGDSMALTLTVSSSSPSLAVLWTVEHLKGQIQIHVQEGKDTHLRVTDTISFTDVHSIVRYLARLAPDKGLSGSHLTEQTEVSHWMEFSQTKLMQKGEFSTGLQELNHCLSLRTYLVGNALTLADICVFSALEASSEWQALVQKGQTPVNVRRWYSFLEPQLGFWSRTNNKVPSEKKQDVGKFVELPGAEMGKVIVRFPPEASGYLHIGHAKAALLNQHYQVTFKGKLIMRFDDTNPEKEKEDFEKVILEDVSMLQIKPDQFTYTSDHFDTIMKYAEKLIQEGKAYVDDTPAEQMKSEREQRIESKHRSNTVAKNMEMWEDMKKGTEYGQTCCLRAKIDMNSNNGCMRDPTLFRCKNQPHPRTGSKYNIYPTYDFACPIVDSVEGVTHALRTTEYHDRDEQFYWIIDALGIRKPYIWEYSRLNLNNTVLSKRRLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYSALLKSQAVAVNIPDAKEGMVEVAKHPKNAEVGQKPVWYGPKVLIEGADAETLSEGETVTFINWGNLIITKIHRDQSGKIQSLDAKLNLENKDYKKTTKITWLAETPKAPFIPTSCVNYDHLITKPVLGKEEDFKQYVNRSSKQEEVMLGDPCLKDLKKGDLIQLQRRGFYICDEPYEPISPHSCKEAPCVLIYIPDGHTKEMPTSGSKEKSKTEATKKETQPKSKGKAANVPVSVAPLASPEEDPVALYNKVSAQGDLVRDLKSKKAAKAEVDEAVKLLLSLKAQYKEVTGQEYKPGSAPAQKPAPSPAASSSGDAKAIYEKVSAQGETVRKLKTEKAPKDKVDEAVKLLLALKAEYKEKTGQEYKPGQTPAAAPTKKALEKPTSSQANGSHSAEAEELYNRVARQGEEVRKVKSEKAAKDKVDAAVKELLDLKAQYKALTGTEYKPVAAPSSEEKKHKEKENKSEKQSKQSKAGEGPKKKDTSKDQGDNSAAGESQGPKKQTRLGLEAKKEENLPDWYSQVITKSEMIEYYDVSGCYVLRPWAFAIWEAIKEFFDKEIKKLGVENCYFPMFVSQAALEKEKTHIADFAPEVAWVTRSGKTELAEPIAVRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHTAFATYEEAAEEVLQILDLYARVYEDLLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASSRAIQGATSHHLGQNFSKMFEIVFEDPKTPGLKQYAYQNSWGLSTRTIGAMTMVHADNIGMVLPPRVACVQVIIIPCGITNTLSEEDKDALLKKCDQYLKRLLNVDVRARADVRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNKQFVAVRRDNGEKQTISEDQAETRLKEILEEIHTNLYNRALSDLTSNMVVANTMEDFQKNLEKGRIVQIPFCGEIECEDWIKKVTARDQDLEPGAPSMGAKSLCIPFKPLSELQPSAKCVCEKNAAKFYTLFGRSY

Target ORF information:

RefSeq Version	XM_031903115.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase 1 (eprs1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903115.1

ORF Insert Sequence:

ATGTCTACTA GCCCTTCCAC ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATAAAGTGG ATGAGGCTGT GAAACTGCTA 
CTGGCTCTGA AGGCTGAATA TAAGGAGAAG ACAGGGCAGG AGTATAAACC AGGACAGACC 
CCGGCTGCTG CTCCCACAAA AAAGGCCCTG GAAAAACCCA CAAGTTCTCA GGCAAATGGC 
TCACACAGCG CCGAGGCAGA AGAGCTGTAT AACAGAGTGG CCAGACAGGG GGAGGAAGTA 
CGCAAAGTGA AGTCTGAGAA AGCCGCAAAG GACAAAGTGG ATGCTGCCGT AAAGGAGCTT 
CTAGATTTAA AGGCTCAATA CAAAGCTTTG ACGGGCACAG AGTACAAACC AGTGGCTGCC 
CCAAGCTCAG AGGAAAAAAA GCACAAGGAG AAGGAGAACA AGTCTGAGAA GCAAAGCAAG 
CAATCAAAGG CTGGAGAAGG GCCCAAGAAG AAGGATACTT CCAAAGATCA AGGGGACAAT 
TCTGCTGCTG GAGAAAGTCA AGGACCTAAA AAACAGACCA GGTTGGGATT GGAGGCTAAA 
AAGGAAGAGA ACTTACCAGA CTGGTATTCC CAGGTGATCA CCAAATCAGA GATGATCGAA 
TACTATGACG TCAGTGGCTG TTACGTGCTG CGTCCCTGGG CCTTTGCCAT CTGGGAGGCC 
ATTAAAGAGT TCTTTGATAA AGAGATAAAG AAGCTCGGTG TGGAAAACTG TTACTTCCCC 
ATGTTCGTGT CTCAGGCTGC GCTGGAGAAG GAAAAAACCC ACATTGCAGA TTTCGCTCCA 
GAAGTTGCCT GGGTTACAAG GTCAGGAAAG ACAGAGCTAG CAGAACCAAT TGCTGTACGG 
CCCACAAGTG AAACTGTGAT GTATCCCGCT TATGCCAAAT GGGTGCAGTC CCACCGAGAT 
CTCCCCATTA AGCTGAATCA GTGGTGTAAT GTAGTGAGGT GGGAATTTAA ACACCCTCAG 
CCTTTCCTCC GAACACGTGA GTTCCTTTGG CAAGAAGGAC ATACAGCGTT CGCTACATAC 
GAGGAAGCTG CAGAAGAGGT CTTGCAGATA CTGGATCTTT ATGCCCGTGT CTATGAGGAT 
CTCTTGGCTA TTCCTGTTGT AAAAGGAAGA AAGACTGAAA AAGAAAAGTT TGCAGGTGGG 
GACTACACAA CCACAGTGGA AGCATTTATA TCGGCCAGCA GCCGTGCCAT ACAGGGTGCA 
ACATCACATC ACCTTGGCCA GAACTTTTCC AAAATGTTTG AAATTGTCTT TGAAGACCCT 
AAAACTCCTG GGTTAAAACA GTATGCGTAC CAGAACTCAT GGGGTCTTTC AACTCGCACA 
ATAGGAGCAA TGACCATGGT TCATGCAGAC AACATTGGGA TGGTGCTCCC ACCCAGAGTA 
GCCTGTGTGC AGGTAATAAT AATCCCTTGT GGCATCACAA ACACCCTTTC CGAGGAAGAC 
AAAGATGCCC TCCTAAAGAA ATGTGACCAG TATCTGAAGC GCCTTCTCAA CGTTGATGTA 
CGGGCACGCG CAGATGTGAG GGACAACTAC TCCCCGGGAT GGAAGTTCAA TCACTGGGAG 
CTCAAGGGTG TTCCAATCAG GCTTGAGGTT GGGCCACGAG ACATGAAAAA TAAGCAGTTT 
GTAGCAGTCA GAAGAGACAA TGGAGAGAAA CAAACCATCT CAGAGGACCA GGCCGAGACG 
CGGTTAAAGG AAATCTTGGA GGAAATTCAC ACAAATCTAT ATAACAGGGC GCTCAGTGAT 
CTGACAAGTA ACATGGTGGT AGCCAACACG ATGGAAGACT TCCAGAAGAA TTTGGAGAAG 
GGAAGGATTG TACAGATCCC ATTCTGCGGG GAAATTGAAT GTGAAGATTG GATAAAGAAG 
GTAACAGCAA GGGATCAAGA TCTGGAGCCA GGAGCACCTT CCATGGGAGC CAAAAGCCTC 
TGTATCCCCT TCAAACCTCT CAGTGAGCTG CAGCCCAGCG CCAAGTGTGT CTGCGAGAAG 
AACGCCGCCA AGTTCTACAC ACTATTTGGC CGCAGTTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eprs1 ( XM_002936017.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936063.2 Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X1, mRNA.

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CloneID	OXa10927
Clone ID Related Accession (Same CDS sequence)	XM_002936017.3
Accession Version	XM_002936017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bifunctional glutamate/proline--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002936017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACTA GCCCTTCCAG ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATCAGGTAG ATGGAGCTGT GAAAACCCTT 
CTAGCTTTAA AAGCAGAATA TAAACAAGTA ACAGGTCAGG AGTACAAGCC AGGGAGCCCT 
CCAGTAGTGC CAAGCAGCTC TTCTCCACCT CCCGCTAGTC AGAGCTTCTC CAATACCTCA 
GATCTGATAT TGTACAACAG AGTGGTTGAG CAAGGAGAGG TGGTCCGACA GCTCAAAAGC 
AAGAAAGCCC CCAAGGATGA GGTAGATGCA GCCGTGAAAC TTCTTTTGAA ACTTAAAGCT 
GAATACAAGC AATTGACTGG CCAGGAATAT AAGCCTCAGA CTCCACCTTT ATCTCAGCCA 
TCAAGTGAAC CCTCAAAAGT TTTGTCTGGT TCAGAGCCAG CGGCCCTCAG TTCTACTGAC 
TGTGCAAGTC TGTATGAGAA AGTAGCTGAG CAAGGCGATC TGGTCCGCAA GCTTAAATCT 
GAGAAAGCGC CAAAGGATAA AGTGGATGAG GCTGTGAAAC TGCTACTGGC TCTGAAGGCT 
GAATATAAGG AGAAGACAGG GCAGGAGTAT AAACCAGGAC AGACCCCGGC TGCTGCTCCC 
ACAAAAAAGG CCCTGGAAAA ACCCACAAGT TCTCAGGCAA ATGGCTCACA CAGCGCCGAG 
GCAGAAGAGC TGTATAACAG AGTGGCCAGA CAGGGGGAGG AAGTACGCAA AGTGAAGTCT 
GAGAAAGCCG CAAAGGACAA AGTGGATGCT GCCGTAAAGG AGCTTCTAGA TTTAAAGGCT 
CAATACAAAG CTTTGACGGG CACAGAGTAC AAACCAGTGG CTGCCCCAAG CTCAGAGGAA 
AAAAAGCACA AGGAGAAGGA GAACAAGTCT GAGAAGCAAA GCAAGCAATC AAAGGCTGGA 
GAAGGGCCCA AGAAGAAGGA TACTTCCAAA GATCAAGGGG ACAATTCTGC TGCTGGAGAA 
AGTCAAGGAC CTAAAAAACA GACCAGGTTG GGATTGGAGG CTAAAAAGGA AGAGAACTTA 
CCAGACTGGT ATTCCCAGGT GATCACCAAA TCAGAGATGA TCGAATACTA TGACGTCAGT 
GGCTGTTACG TGCTGCGTCC CTGGGCCTTT GCCATCTGGG AGGCCATTAA AGAGTTCTTT 
GATAAAGAGA TAAAGAAGCT CGGTGTGGAA AACTGTTACT TCCCCATGTT CGTGTCTCAG 
GCTGCGCTGG AGAAGGAAAA AACCCACATT GCAGATTTCG CTCCAGAAGT TGCCTGGGTT 
ACAAGGTCAG GAAAGACAGA GCTAGCAGAA CCAATTGCTG TACGGCCCAC AAGTGAAACT 
GTGATGTATC CCGCTTATGC CAAATGGGTG CAGTCCCACC GAGATCTCCC CATTAAGCTG 
AATCAGTGGT GTAATGTAGT GAGGTGGGAA TTTAAACACC CTCAGCCTTT CCTCCGAACA 
CGTGAGTTCC TTTGGCAAGA AGGACATACA GCGTTCGCTA CATACGAGGA AGCTGCAGAA 
GAGGTCTTGC AGATACTGGA TCTTTATGCC CGTGTCTATG AGGATCTCTT GGCTATTCCT 
GTTGTAAAAG GAAGAAAGAC TGAAAAAGAA AAGTTTGCAG GTGGGGACTA CACAACCACA 
GTGGAAGCAT TTATATCGGC CAGCAGCCGT GCCATACAGG GTGCAACATC ACATCACCTT 
GGCCAGAACT TTTCCAAAAT GTTTGAAATT GTCTTTGAAG ACCCTAAAAC TCCTGGGTTA 
AAACAGTATG CGTACCAGAA CTCATGGGGT CTTTCAACTC GCACAATAGG AGCAATGACC 
ATGGTTCATG CAGACAACAT TGGGATGGTG CTCCCACCCA GAGTAGCCTG TGTGCAGGTA 
ATAATAATCC CTTGTGGCAT CACAAACACC CTTTCCGAGG AAGACAAAGA TGCCCTCCTA 
AAGAAATGTG ACCAGTATCT GAAGCGCCTT CTCAACGTTG ATGTACGGGC ACGCGCAGAT 
GTGAGGGACA ACTACTCCCC GGGATGGAAG TTCAATCACT GGGAGCTCAA GGGTGTTCCA 
ATCAGGCTTG AGGTTGGGCC ACGAGACATG AAAAATAAGC AGTTTGTAGC AGTCAGAAGA 
GACAATGGAG AGAAACAAAC CATCTCAGAG GACCAGGCCG AGACGCGGTT AAAGGAAATC 
TTGGAGGAAA TTCACACAAA TCTATATAAC AGGGCGCTCA GTGATCTGAC AAGTAACATG 
GTGGTAGCCA ACACGATGGA AGACTTCCAG AAGAATTTGG AGAAGGGAAG GATTGTACAG 
ATCCCATTCT GCGGGGAAAT TGAATGTGAA GATTGGATAA AGAAGGTAAC AGCAAGGGAT 
CAAGATCTGG AGCCAGGAGC ACCTTCCATG GGAGCCAAAA GCCTCTGTAT CCCCTTCAAA 
CCTCTCAGTG AGCTGCAGCC CAGCGCCAAG TGTGTCTGCG AGAAGAACGC CGCCAAGTTC 
TACACACTAT TTGGCCGCAG TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936063.2
CDS	42..5168
Translation	MSTSPSRQEVRLLPGQCWSVCEPEAGCTLSHSARSFVAAAGISSSIAGGDSMALTLTVSSSSPSLAVLWTVEHLKGQIQIHVQEGKDTHLRVTDTISFTDVHSIVRYLARLAPDKGLSGSHLTEQTEVSHWMEFSQTKLMQKGEFSTGLQELNHCLSLRTYLVGNALTLADICVFSALEASSEWQALVQKGQTPVNVRRWYSFLEPQLGFWSRTNNKVPSEKKQDVGKFVELPGAEMGKVIVRFPPEASGYLHIGHAKAALLNQHYQVTFKGKLIMRFDDTNPEKEKEDFEKVILEDVSMLQIKPDQFTYTSDHFDTIMKYAEKLIQEGKAYVDDTPAEQMKSEREQRIESKHRSNTVAKNMEMWEDMKKGTEYGQTCCLRAKIDMNSNNGCMRDPTLFRCKNQPHPRTGSKYNIYPTYDFACPIVDSVEGVTHALRTTEYHDRDEQFYWIIDALGIRKPYIWEYSRLNLNNTVLSKRRLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYSALLKSQAVAVNIPDAKEGMVEVAKHPKNAEVGQKPVWYGPKVLIEGADAETLSEGETVTFINWGNLIITKIHRDQSGKIQSLDAKLNLENKDYKKTTKITWLAETPKAPFIPTSCVNYDHLITKPVLGKEEDFKQYVNRSSKQEEVMLGDPCLKDLKKGDLIQLQRRGFYICDEPYEPISPHSCKEAPCVLIYIPDGHTKEMPTSGSKEKSKTEATKKETQPKSKGKAANVPVSVAPLASPEEDPVALYNKVSAQGDLVRDLKSKKAAKAEVDEAVKLLLSLKAQYKEVTGQEYKPGSAPAQKPAPSPAASSSGDAKAIYEKVSAQGETVRKLKTEKAPKDQVDGAVKTLLALKAEYKQVTGQEYKPGSPPVVPSSSSPPPASQSFSNTSDLILYNRVVEQGEVVRQLKSKKAPKDEVDAAVKLLLKLKAEYKQLTGQEYKPQTPPLSQPSSEPSKVLSGSEPAALSSTDCASLYEKVAEQGDLVRKLKSEKAPKDKVDEAVKLLLALKAEYKEKTGQEYKPGQTPAAAPTKKALEKPTSSQANGSHSAEAEELYNRVARQGEEVRKVKSEKAAKDKVDAAVKELLDLKAQYKALTGTEYKPVAAPSSEEKKHKEKENKSEKQSKQSKAGEGPKKKDTSKDQGDNSAAGESQGPKKQTRLGLEAKKEENLPDWYSQVITKSEMIEYYDVSGCYVLRPWAFAIWEAIKEFFDKEIKKLGVENCYFPMFVSQAALEKEKTHIADFAPEVAWVTRSGKTELAEPIAVRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHTAFATYEEAAEEVLQILDLYARVYEDLLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASSRAIQGATSHHLGQNFSKMFEIVFEDPKTPGLKQYAYQNSWGLSTRTIGAMTMVHADNIGMVLPPRVACVQVIIIPCGITNTLSEEDKDALLKKCDQYLKRLLNVDVRARADVRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNKQFVAVRRDNGEKQTISEDQAETRLKEILEEIHTNLYNRALSDLTSNMVVANTMEDFQKNLEKGRIVQIPFCGEIECEDWIKKVTARDQDLEPGAPSMGAKSLCIPFKPLSELQPSAKCVCEKNAAKFYTLFGRSY

Target ORF information:

RefSeq Version	XM_002936017.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936017.3

ORF Insert Sequence:

ATGTCTACTA GCCCTTCCAG ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATCAGGTAG ATGGAGCTGT GAAAACCCTT 
CTAGCTTTAA AAGCAGAATA TAAACAAGTA ACAGGTCAGG AGTACAAGCC AGGGAGCCCT 
CCAGTAGTGC CAAGCAGCTC TTCTCCACCT CCCGCTAGTC AGAGCTTCTC CAATACCTCA 
GATCTGATAT TGTACAACAG AGTGGTTGAG CAAGGAGAGG TGGTCCGACA GCTCAAAAGC 
AAGAAAGCCC CCAAGGATGA GGTAGATGCA GCCGTGAAAC TTCTTTTGAA ACTTAAAGCT 
GAATACAAGC AATTGACTGG CCAGGAATAT AAGCCTCAGA CTCCACCTTT ATCTCAGCCA 
TCAAGTGAAC CCTCAAAAGT TTTGTCTGGT TCAGAGCCAG CGGCCCTCAG TTCTACTGAC 
TGTGCAAGTC TGTATGAGAA AGTAGCTGAG CAAGGCGATC TGGTCCGCAA GCTTAAATCT 
GAGAAAGCGC CAAAGGATAA AGTGGATGAG GCTGTGAAAC TGCTACTGGC TCTGAAGGCT 
GAATATAAGG AGAAGACAGG GCAGGAGTAT AAACCAGGAC AGACCCCGGC TGCTGCTCCC 
ACAAAAAAGG CCCTGGAAAA ACCCACAAGT TCTCAGGCAA ATGGCTCACA CAGCGCCGAG 
GCAGAAGAGC TGTATAACAG AGTGGCCAGA CAGGGGGAGG AAGTACGCAA AGTGAAGTCT 
GAGAAAGCCG CAAAGGACAA AGTGGATGCT GCCGTAAAGG AGCTTCTAGA TTTAAAGGCT 
CAATACAAAG CTTTGACGGG CACAGAGTAC AAACCAGTGG CTGCCCCAAG CTCAGAGGAA 
AAAAAGCACA AGGAGAAGGA GAACAAGTCT GAGAAGCAAA GCAAGCAATC AAAGGCTGGA 
GAAGGGCCCA AGAAGAAGGA TACTTCCAAA GATCAAGGGG ACAATTCTGC TGCTGGAGAA 
AGTCAAGGAC CTAAAAAACA GACCAGGTTG GGATTGGAGG CTAAAAAGGA AGAGAACTTA 
CCAGACTGGT ATTCCCAGGT GATCACCAAA TCAGAGATGA TCGAATACTA TGACGTCAGT 
GGCTGTTACG TGCTGCGTCC CTGGGCCTTT GCCATCTGGG AGGCCATTAA AGAGTTCTTT 
GATAAAGAGA TAAAGAAGCT CGGTGTGGAA AACTGTTACT TCCCCATGTT CGTGTCTCAG 
GCTGCGCTGG AGAAGGAAAA AACCCACATT GCAGATTTCG CTCCAGAAGT TGCCTGGGTT 
ACAAGGTCAG GAAAGACAGA GCTAGCAGAA CCAATTGCTG TACGGCCCAC AAGTGAAACT 
GTGATGTATC CCGCTTATGC CAAATGGGTG CAGTCCCACC GAGATCTCCC CATTAAGCTG 
AATCAGTGGT GTAATGTAGT GAGGTGGGAA TTTAAACACC CTCAGCCTTT CCTCCGAACA 
CGTGAGTTCC TTTGGCAAGA AGGACATACA GCGTTCGCTA CATACGAGGA AGCTGCAGAA 
GAGGTCTTGC AGATACTGGA TCTTTATGCC CGTGTCTATG AGGATCTCTT GGCTATTCCT 
GTTGTAAAAG GAAGAAAGAC TGAAAAAGAA AAGTTTGCAG GTGGGGACTA CACAACCACA 
GTGGAAGCAT TTATATCGGC CAGCAGCCGT GCCATACAGG GTGCAACATC ACATCACCTT 
GGCCAGAACT TTTCCAAAAT GTTTGAAATT GTCTTTGAAG ACCCTAAAAC TCCTGGGTTA 
AAACAGTATG CGTACCAGAA CTCATGGGGT CTTTCAACTC GCACAATAGG AGCAATGACC 
ATGGTTCATG CAGACAACAT TGGGATGGTG CTCCCACCCA GAGTAGCCTG TGTGCAGGTA 
ATAATAATCC CTTGTGGCAT CACAAACACC CTTTCCGAGG AAGACAAAGA TGCCCTCCTA 
AAGAAATGTG ACCAGTATCT GAAGCGCCTT CTCAACGTTG ATGTACGGGC ACGCGCAGAT 
GTGAGGGACA ACTACTCCCC GGGATGGAAG TTCAATCACT GGGAGCTCAA GGGTGTTCCA 
ATCAGGCTTG AGGTTGGGCC ACGAGACATG AAAAATAAGC AGTTTGTAGC AGTCAGAAGA 
GACAATGGAG AGAAACAAAC CATCTCAGAG GACCAGGCCG AGACGCGGTT AAAGGAAATC 
TTGGAGGAAA TTCACACAAA TCTATATAAC AGGGCGCTCA GTGATCTGAC AAGTAACATG 
GTGGTAGCCA ACACGATGGA AGACTTCCAG AAGAATTTGG AGAAGGGAAG GATTGTACAG 
ATCCCATTCT GCGGGGAAAT TGAATGTGAA GATTGGATAA AGAAGGTAAC AGCAAGGGAT 
CAAGATCTGG AGCCAGGAGC ACCTTCCATG GGAGCCAAAA GCCTCTGTAT CCCCTTCAAA 
CCTCTCAGTG AGCTGCAGCC CAGCGCCAAG TGTGTCTGCG AGAAGAACGC CGCCAAGTTC 
TACACACTAT TTGGCCGCAG TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eprs1 ( XM_002936018.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936064.2 Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X4, mRNA.

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CloneID	OXa10928
Clone ID Related Accession (Same CDS sequence)	XM_002936018.3
Accession Version	XM_002936018.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bifunctional glutamate/proline--tRNA ligase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002936018.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACTA GCCCTTCCAG ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATAAAGTGG ATGAGGCTGT GAAACTGCTA 
CTGGCTCTGA AGGCTGAATA TAAGGAGAAG ACAGGGCAGG AGTATAAACC AGGACAGACC 
CCGGCTGCTG CTCCCACAAA AAAGGCCCTG GAAAAACCCA CAAGTTCTCA GGCAAATGGC 
TCACACAGCG CCGAGGCAGA AGAGCTGTAT AACAGAGTGG CCAGACAGGG GGAGGAAGTA 
CGCAAAGTGA AGTCTGAGAA AGCCGCAAAG GACAAAGTGG ATGCTGCCGT AAAGGAGCTT 
CTAGATTTAA AGGCTCAATA CAAAGCTTTG ACGGGCACAG AGTACAAACC AGTGGCTGCC 
CCAAGCTCAG AGGAAAAAAA GCACAAGGAG AAGGAGAACA AGTCTGAGAA GCAAAGCAAG 
CAATCAAAGG CTGGAGAAGG GCCCAAGAAG AAGGATACTT CCAAAGATCA AGGGGACAAT 
TCTGCTGCTG GAGAAAGTCA AGGACCTAAA AAACAGACCA GGTTGGGATT GGAGGCTAAA 
AAGGAAGAGA ACTTACCAGA CTGGTATTCC CAGGTGATCA CCAAATCAGA GATGATCGAA 
TACTATGACG TCAGTGGCTG TTACGTGCTG CGTCCCTGGG CCTTTGCCAT CTGGGAGGCC 
ATTAAAGAGT TCTTTGATAA AGAGATAAAG AAGCTCGGTG TGGAAAACTG TTACTTCCCC 
ATGTTCGTGT CTCAGGCTGC GCTGGAGAAG GAAAAAACCC ACATTGCAGA TTTCGCTCCA 
GAAGTTGCCT GGGTTACAAG GTCAGGAAAG ACAGAGCTAG CAGAACCAAT TGCTGTACGG 
CCCACAAGTG AAACTGTGAT GTATCCCGCT TATGCCAAAT GGGTGCAGTC CCACCGAGAT 
CTCCCCATTA AGCTGAATCA GTGGTGTAAT GTAGTGAGGT GGGAATTTAA ACACCCTCAG 
CCTTTCCTCC GAACACGTGA GTTCCTTTGG CAAGAAGGAC ATACAGCGTT CGCTACATAC 
GAGGAAGCTG CAGAAGAGGT CTTGCAGATA CTGGATCTTT ATGCCCGTGT CTATGAGGAT 
CTCTTGGCTA TTCCTGTTGT AAAAGGAAGA AAGACTGAAA AAGAAAAGTT TGCAGGTGGG 
GACTACACAA CCACAGTGGA AGCATTTATA TCGGCCAGCA GCCGTGCCAT ACAGGGTGCA 
ACATCACATC ACCTTGGCCA GAACTTTTCC AAAATGTTTG AAATTGTCTT TGAAGACCCT 
AAAACTCCTG GGTTAAAACA GTATGCGTAC CAGAACTCAT GGGGTCTTTC AACTCGCACA 
ATAGGAGCAA TGACCATGGT TCATGCAGAC AACATTGGGA TGGTGCTCCC ACCCAGAGTA 
GCCTGTGTGC AGGTAATAAT AATCCCTTGT GGCATCACAA ACACCCTTTC CGAGGAAGAC 
AAAGATGCCC TCCTAAAGAA ATGTGACCAG TATCTGAAGC GCCTTCTCAA CGTTGATGTA 
CGGGCACGCG CAGATGTGAG GGACAACTAC TCCCCGGGAT GGAAGTTCAA TCACTGGGAG 
CTCAAGGGTG TTCCAATCAG GCTTGAGGTT GGGCCACGAG ACATGAAAAA TAAGCAGTTT 
GTAGCAGTCA GAAGAGACAA TGGAGAGAAA CAAACCATCT CAGAGGACCA GGCCGAGACG 
CGGTTAAAGG AAATCTTGGA GGAAATTCAC ACAAATCTAT ATAACAGGGC GCTCAGTGAT 
CTGACAAGTA ACATGGTGGT AGCCAACACG ATGGAAGACT TCCAGAAGAA TTTGGAGAAG 
GGAAGGATTG TACAGATCCC ATTCTGCGGG GAAATTGAAT GTGAAGATTG GATAAAGAAG 
GTAACAGCAA GGGATCAAGA TCTGGAGCCA GGAGCACCTT CCATGGGAGC CAAAAGCCTC 
TGTATCCCCT TCAAACCTCT CAGTGAGCTG CAGCCCAGCG CCAAGTGTGT CTGCGAGAAG 
AACGCCGCCA AGTTCTACAC ACTATTTGGC CGCAGTTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936064.2
CDS	42..4703
Translation	MSTSPSRQEVRLLPGQCWSVCEPEAGCTLSHSARSFVAAAGISSSIAGGDSMALTLTVSSSSPSLAVLWTVEHLKGQIQIHVQEGKDTHLRVTDTISFTDVHSIVRYLARLAPDKGLSGSHLTEQTEVSHWMEFSQTKLMQKGEFSTGLQELNHCLSLRTYLVGNALTLADICVFSALEASSEWQALVQKGQTPVNVRRWYSFLEPQLGFWSRTNNKVPSEKKQDVGKFVELPGAEMGKVIVRFPPEASGYLHIGHAKAALLNQHYQVTFKGKLIMRFDDTNPEKEKEDFEKVILEDVSMLQIKPDQFTYTSDHFDTIMKYAEKLIQEGKAYVDDTPAEQMKSEREQRIESKHRSNTVAKNMEMWEDMKKGTEYGQTCCLRAKIDMNSNNGCMRDPTLFRCKNQPHPRTGSKYNIYPTYDFACPIVDSVEGVTHALRTTEYHDRDEQFYWIIDALGIRKPYIWEYSRLNLNNTVLSKRRLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYSALLKSQAVAVNIPDAKEGMVEVAKHPKNAEVGQKPVWYGPKVLIEGADAETLSEGETVTFINWGNLIITKIHRDQSGKIQSLDAKLNLENKDYKKTTKITWLAETPKAPFIPTSCVNYDHLITKPVLGKEEDFKQYVNRSSKQEEVMLGDPCLKDLKKGDLIQLQRRGFYICDEPYEPISPHSCKEAPCVLIYIPDGHTKEMPTSGSKEKSKTEATKKETQPKSKGKAANVPVSVAPLASPEEDPVALYNKVSAQGDLVRDLKSKKAAKAEVDEAVKLLLSLKAQYKEVTGQEYKPGSAPAQKPAPSPAASSSGDAKAIYEKVSAQGETVRKLKTEKAPKDKVDEAVKLLLALKAEYKEKTGQEYKPGQTPAAAPTKKALEKPTSSQANGSHSAEAEELYNRVARQGEEVRKVKSEKAAKDKVDAAVKELLDLKAQYKALTGTEYKPVAAPSSEEKKHKEKENKSEKQSKQSKAGEGPKKKDTSKDQGDNSAAGESQGPKKQTRLGLEAKKEENLPDWYSQVITKSEMIEYYDVSGCYVLRPWAFAIWEAIKEFFDKEIKKLGVENCYFPMFVSQAALEKEKTHIADFAPEVAWVTRSGKTELAEPIAVRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHTAFATYEEAAEEVLQILDLYARVYEDLLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASSRAIQGATSHHLGQNFSKMFEIVFEDPKTPGLKQYAYQNSWGLSTRTIGAMTMVHADNIGMVLPPRVACVQVIIIPCGITNTLSEEDKDALLKKCDQYLKRLLNVDVRARADVRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNKQFVAVRRDNGEKQTISEDQAETRLKEILEEIHTNLYNRALSDLTSNMVVANTMEDFQKNLEKGRIVQIPFCGEIECEDWIKKVTARDQDLEPGAPSMGAKSLCIPFKPLSELQPSAKCVCEKNAAKFYTLFGRSY

Target ORF information:

RefSeq Version	XM_002936018.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936018.3

ORF Insert Sequence:

ATGTCTACTA GCCCTTCCAG ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATAAAGTGG ATGAGGCTGT GAAACTGCTA 
CTGGCTCTGA AGGCTGAATA TAAGGAGAAG ACAGGGCAGG AGTATAAACC AGGACAGACC 
CCGGCTGCTG CTCCCACAAA AAAGGCCCTG GAAAAACCCA CAAGTTCTCA GGCAAATGGC 
TCACACAGCG CCGAGGCAGA AGAGCTGTAT AACAGAGTGG CCAGACAGGG GGAGGAAGTA 
CGCAAAGTGA AGTCTGAGAA AGCCGCAAAG GACAAAGTGG ATGCTGCCGT AAAGGAGCTT 
CTAGATTTAA AGGCTCAATA CAAAGCTTTG ACGGGCACAG AGTACAAACC AGTGGCTGCC 
CCAAGCTCAG AGGAAAAAAA GCACAAGGAG AAGGAGAACA AGTCTGAGAA GCAAAGCAAG 
CAATCAAAGG CTGGAGAAGG GCCCAAGAAG AAGGATACTT CCAAAGATCA AGGGGACAAT 
TCTGCTGCTG GAGAAAGTCA AGGACCTAAA AAACAGACCA GGTTGGGATT GGAGGCTAAA 
AAGGAAGAGA ACTTACCAGA CTGGTATTCC CAGGTGATCA CCAAATCAGA GATGATCGAA 
TACTATGACG TCAGTGGCTG TTACGTGCTG CGTCCCTGGG CCTTTGCCAT CTGGGAGGCC 
ATTAAAGAGT TCTTTGATAA AGAGATAAAG AAGCTCGGTG TGGAAAACTG TTACTTCCCC 
ATGTTCGTGT CTCAGGCTGC GCTGGAGAAG GAAAAAACCC ACATTGCAGA TTTCGCTCCA 
GAAGTTGCCT GGGTTACAAG GTCAGGAAAG ACAGAGCTAG CAGAACCAAT TGCTGTACGG 
CCCACAAGTG AAACTGTGAT GTATCCCGCT TATGCCAAAT GGGTGCAGTC CCACCGAGAT 
CTCCCCATTA AGCTGAATCA GTGGTGTAAT GTAGTGAGGT GGGAATTTAA ACACCCTCAG 
CCTTTCCTCC GAACACGTGA GTTCCTTTGG CAAGAAGGAC ATACAGCGTT CGCTACATAC 
GAGGAAGCTG CAGAAGAGGT CTTGCAGATA CTGGATCTTT ATGCCCGTGT CTATGAGGAT 
CTCTTGGCTA TTCCTGTTGT AAAAGGAAGA AAGACTGAAA AAGAAAAGTT TGCAGGTGGG 
GACTACACAA CCACAGTGGA AGCATTTATA TCGGCCAGCA GCCGTGCCAT ACAGGGTGCA 
ACATCACATC ACCTTGGCCA GAACTTTTCC AAAATGTTTG AAATTGTCTT TGAAGACCCT 
AAAACTCCTG GGTTAAAACA GTATGCGTAC CAGAACTCAT GGGGTCTTTC AACTCGCACA 
ATAGGAGCAA TGACCATGGT TCATGCAGAC AACATTGGGA TGGTGCTCCC ACCCAGAGTA 
GCCTGTGTGC AGGTAATAAT AATCCCTTGT GGCATCACAA ACACCCTTTC CGAGGAAGAC 
AAAGATGCCC TCCTAAAGAA ATGTGACCAG TATCTGAAGC GCCTTCTCAA CGTTGATGTA 
CGGGCACGCG CAGATGTGAG GGACAACTAC TCCCCGGGAT GGAAGTTCAA TCACTGGGAG 
CTCAAGGGTG TTCCAATCAG GCTTGAGGTT GGGCCACGAG ACATGAAAAA TAAGCAGTTT 
GTAGCAGTCA GAAGAGACAA TGGAGAGAAA CAAACCATCT CAGAGGACCA GGCCGAGACG 
CGGTTAAAGG AAATCTTGGA GGAAATTCAC ACAAATCTAT ATAACAGGGC GCTCAGTGAT 
CTGACAAGTA ACATGGTGGT AGCCAACACG ATGGAAGACT TCCAGAAGAA TTTGGAGAAG 
GGAAGGATTG TACAGATCCC ATTCTGCGGG GAAATTGAAT GTGAAGATTG GATAAAGAAG 
GTAACAGCAA GGGATCAAGA TCTGGAGCCA GGAGCACCTT CCATGGGAGC CAAAAGCCTC 
TGTATCCCCT TCAAACCTCT CAGTGAGCTG CAGCCCAGCG CCAAGTGTGT CTGCGAGAAG 
AACGCCGCCA AGTTCTACAC ACTATTTGGC CGCAGTTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eprs1 ( XM_012963955.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819409.1 Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X2, mRNA.

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CloneID	OXa31526
Clone ID Related Accession (Same CDS sequence)	XM_012963955.1
Accession Version	XM_012963955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bifunctional glutamate/proline--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACTA GCCCTTCCAG ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATGAGGTAG ATGCAGCCGT GAAACTTCTT 
TTGAAACTTA AAGCTGAATA CAAGCAATTG ACTGGCCAGG AATATAAGCC TCAGACTCCA 
CCTTTATCTC AGCCATCAAG TGAACCCTCA AAAGTTTTGT CTGGTTCAGA GCCAGCGGCC 
CTCAGTTCTA CTGACTGTGC AAGTCTGTAT GAGAAAGTAG CTGAGCAAGG CGATCTGGTC 
CGCAAGCTTA AATCTGAGAA AGCGCCAAAG GATAAAGTGG ATGAGGCTGT GAAACTGCTA 
CTGGCTCTGA AGGCTGAATA TAAGGAGAAG ACAGGGCAGG AGTATAAACC AGGACAGACC 
CCGGCTGCTG CTCCCACAAA AAAGGCCCTG GAAAAACCCA CAAGTTCTCA GGCAAATGGC 
TCACACAGCG CCGAGGCAGA AGAGCTGTAT AACAGAGTGG CCAGACAGGG GGAGGAAGTA 
CGCAAAGTGA AGTCTGAGAA AGCCGCAAAG GACAAAGTGG ATGCTGCCGT AAAGGAGCTT 
CTAGATTTAA AGGCTCAATA CAAAGCTTTG ACGGGCACAG AGTACAAACC AGTGGCTGCC 
CCAAGCTCAG AGGAAAAAAA GCACAAGGAG AAGGAGAACA AGTCTGAGAA GCAAAGCAAG 
CAATCAAAGG CTGGAGAAGG GCCCAAGAAG AAGGATACTT CCAAAGATCA AGGGGACAAT 
TCTGCTGCTG GAGAAAGTCA AGGACCTAAA AAACAGACCA GGTTGGGATT GGAGGCTAAA 
AAGGAAGAGA ACTTACCAGA CTGGTATTCC CAGGTGATCA CCAAATCAGA GATGATCGAA 
TACTATGACG TCAGTGGCTG TTACGTGCTG CGTCCCTGGG CCTTTGCCAT CTGGGAGGCC 
ATTAAAGAGT TCTTTGATAA AGAGATAAAG AAGCTCGGTG TGGAAAACTG TTACTTCCCC 
ATGTTCGTGT CTCAGGCTGC GCTGGAGAAG GAAAAAACCC ACATTGCAGA TTTCGCTCCA 
GAAGTTGCCT GGGTTACAAG GTCAGGAAAG ACAGAGCTAG CAGAACCAAT TGCTGTACGG 
CCCACAAGTG AAACTGTGAT GTATCCCGCT TATGCCAAAT GGGTGCAGTC CCACCGAGAT 
CTCCCCATTA AGCTGAATCA GTGGTGTAAT GTAGTGAGGT GGGAATTTAA ACACCCTCAG 
CCTTTCCTCC GAACACGTGA GTTCCTTTGG CAAGAAGGAC ATACAGCGTT CGCTACATAC 
GAGGAAGCTG CAGAAGAGGT CTTGCAGATA CTGGATCTTT ATGCCCGTGT CTATGAGGAT 
CTCTTGGCTA TTCCTGTTGT AAAAGGAAGA AAGACTGAAA AAGAAAAGTT TGCAGGTGGG 
GACTACACAA CCACAGTGGA AGCATTTATA TCGGCCAGCA GCCGTGCCAT ACAGGGTGCA 
ACATCACATC ACCTTGGCCA GAACTTTTCC AAAATGTTTG AAATTGTCTT TGAAGACCCT 
AAAACTCCTG GGTTAAAACA GTATGCGTAC CAGAACTCAT GGGGTCTTTC AACTCGCACA 
ATAGGAGCAA TGACCATGGT TCATGCAGAC AACATTGGGA TGGTGCTCCC ACCCAGAGTA 
GCCTGTGTGC AGGTAATAAT AATCCCTTGT GGCATCACAA ACACCCTTTC CGAGGAAGAC 
AAAGATGCCC TCCTAAAGAA ATGTGACCAG TATCTGAAGC GCCTTCTCAA CGTTGATGTA 
CGGGCACGCG CAGATGTGAG GGACAACTAC TCCCCGGGAT GGAAGTTCAA TCACTGGGAG 
CTCAAGGGTG TTCCAATCAG GCTTGAGGTT GGGCCACGAG ACATGAAAAA TAAGCAGTTT 
GTAGCAGTCA GAAGAGACAA TGGAGAGAAA CAAACCATCT CAGAGGACCA GGCCGAGACG 
CGGTTAAAGG AAATCTTGGA GGAAATTCAC ACAAATCTAT ATAACAGGGC GCTCAGTGAT 
CTGACAAGTA ACATGGTGGT AGCCAACACG ATGGAAGACT TCCAGAAGAA TTTGGAGAAG 
GGAAGGATTG TACAGATCCC ATTCTGCGGG GAAATTGAAT GTGAAGATTG GATAAAGAAG 
GTAACAGCAA GGGATCAAGA TCTGGAGCCA GGAGCACCTT CCATGGGAGC CAAAAGCCTC 
TGTATCCCCT TCAAACCTCT CAGTGAGCTG CAGCCCAGCG CCAAGTGTGT CTGCGAGAAG 
AACGCCGCCA AGTTCTACAC ACTATTTGGC CGCAGTTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819409.1
CDS	42..4943
Translation	MSTSPSRQEVRLLPGQCWSVCEPEAGCTLSHSARSFVAAAGISSSIAGGDSMALTLTVSSSSPSLAVLWTVEHLKGQIQIHVQEGKDTHLRVTDTISFTDVHSIVRYLARLAPDKGLSGSHLTEQTEVSHWMEFSQTKLMQKGEFSTGLQELNHCLSLRTYLVGNALTLADICVFSALEASSEWQALVQKGQTPVNVRRWYSFLEPQLGFWSRTNNKVPSEKKQDVGKFVELPGAEMGKVIVRFPPEASGYLHIGHAKAALLNQHYQVTFKGKLIMRFDDTNPEKEKEDFEKVILEDVSMLQIKPDQFTYTSDHFDTIMKYAEKLIQEGKAYVDDTPAEQMKSEREQRIESKHRSNTVAKNMEMWEDMKKGTEYGQTCCLRAKIDMNSNNGCMRDPTLFRCKNQPHPRTGSKYNIYPTYDFACPIVDSVEGVTHALRTTEYHDRDEQFYWIIDALGIRKPYIWEYSRLNLNNTVLSKRRLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYSALLKSQAVAVNIPDAKEGMVEVAKHPKNAEVGQKPVWYGPKVLIEGADAETLSEGETVTFINWGNLIITKIHRDQSGKIQSLDAKLNLENKDYKKTTKITWLAETPKAPFIPTSCVNYDHLITKPVLGKEEDFKQYVNRSSKQEEVMLGDPCLKDLKKGDLIQLQRRGFYICDEPYEPISPHSCKEAPCVLIYIPDGHTKEMPTSGSKEKSKTEATKKETQPKSKGKAANVPVSVAPLASPEEDPVALYNKVSAQGDLVRDLKSKKAAKAEVDEAVKLLLSLKAQYKEVTGQEYKPGSAPAQKPAPSPAASSSGDAKAIYEKVSAQGETVRKLKTEKAPKDEVDAAVKLLLKLKAEYKQLTGQEYKPQTPPLSQPSSEPSKVLSGSEPAALSSTDCASLYEKVAEQGDLVRKLKSEKAPKDKVDEAVKLLLALKAEYKEKTGQEYKPGQTPAAAPTKKALEKPTSSQANGSHSAEAEELYNRVARQGEEVRKVKSEKAAKDKVDAAVKELLDLKAQYKALTGTEYKPVAAPSSEEKKHKEKENKSEKQSKQSKAGEGPKKKDTSKDQGDNSAAGESQGPKKQTRLGLEAKKEENLPDWYSQVITKSEMIEYYDVSGCYVLRPWAFAIWEAIKEFFDKEIKKLGVENCYFPMFVSQAALEKEKTHIADFAPEVAWVTRSGKTELAEPIAVRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHTAFATYEEAAEEVLQILDLYARVYEDLLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASSRAIQGATSHHLGQNFSKMFEIVFEDPKTPGLKQYAYQNSWGLSTRTIGAMTMVHADNIGMVLPPRVACVQVIIIPCGITNTLSEEDKDALLKKCDQYLKRLLNVDVRARADVRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNKQFVAVRRDNGEKQTISEDQAETRLKEILEEIHTNLYNRALSDLTSNMVVANTMEDFQKNLEKGRIVQIPFCGEIECEDWIKKVTARDQDLEPGAPSMGAKSLCIPFKPLSELQPSAKCVCEKNAAKFYTLFGRSY

Target ORF information:

RefSeq Version	XM_012963955.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963955.1

ORF Insert Sequence:

ATGTCTACTA GCCCTTCCAG ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATGAGGTAG ATGCAGCCGT GAAACTTCTT 
TTGAAACTTA AAGCTGAATA CAAGCAATTG ACTGGCCAGG AATATAAGCC TCAGACTCCA 
CCTTTATCTC AGCCATCAAG TGAACCCTCA AAAGTTTTGT CTGGTTCAGA GCCAGCGGCC 
CTCAGTTCTA CTGACTGTGC AAGTCTGTAT GAGAAAGTAG CTGAGCAAGG CGATCTGGTC 
CGCAAGCTTA AATCTGAGAA AGCGCCAAAG GATAAAGTGG ATGAGGCTGT GAAACTGCTA 
CTGGCTCTGA AGGCTGAATA TAAGGAGAAG ACAGGGCAGG AGTATAAACC AGGACAGACC 
CCGGCTGCTG CTCCCACAAA AAAGGCCCTG GAAAAACCCA CAAGTTCTCA GGCAAATGGC 
TCACACAGCG CCGAGGCAGA AGAGCTGTAT AACAGAGTGG CCAGACAGGG GGAGGAAGTA 
CGCAAAGTGA AGTCTGAGAA AGCCGCAAAG GACAAAGTGG ATGCTGCCGT AAAGGAGCTT 
CTAGATTTAA AGGCTCAATA CAAAGCTTTG ACGGGCACAG AGTACAAACC AGTGGCTGCC 
CCAAGCTCAG AGGAAAAAAA GCACAAGGAG AAGGAGAACA AGTCTGAGAA GCAAAGCAAG 
CAATCAAAGG CTGGAGAAGG GCCCAAGAAG AAGGATACTT CCAAAGATCA AGGGGACAAT 
TCTGCTGCTG GAGAAAGTCA AGGACCTAAA AAACAGACCA GGTTGGGATT GGAGGCTAAA 
AAGGAAGAGA ACTTACCAGA CTGGTATTCC CAGGTGATCA CCAAATCAGA GATGATCGAA 
TACTATGACG TCAGTGGCTG TTACGTGCTG CGTCCCTGGG CCTTTGCCAT CTGGGAGGCC 
ATTAAAGAGT TCTTTGATAA AGAGATAAAG AAGCTCGGTG TGGAAAACTG TTACTTCCCC 
ATGTTCGTGT CTCAGGCTGC GCTGGAGAAG GAAAAAACCC ACATTGCAGA TTTCGCTCCA 
GAAGTTGCCT GGGTTACAAG GTCAGGAAAG ACAGAGCTAG CAGAACCAAT TGCTGTACGG 
CCCACAAGTG AAACTGTGAT GTATCCCGCT TATGCCAAAT GGGTGCAGTC CCACCGAGAT 
CTCCCCATTA AGCTGAATCA GTGGTGTAAT GTAGTGAGGT GGGAATTTAA ACACCCTCAG 
CCTTTCCTCC GAACACGTGA GTTCCTTTGG CAAGAAGGAC ATACAGCGTT CGCTACATAC 
GAGGAAGCTG CAGAAGAGGT CTTGCAGATA CTGGATCTTT ATGCCCGTGT CTATGAGGAT 
CTCTTGGCTA TTCCTGTTGT AAAAGGAAGA AAGACTGAAA AAGAAAAGTT TGCAGGTGGG 
GACTACACAA CCACAGTGGA AGCATTTATA TCGGCCAGCA GCCGTGCCAT ACAGGGTGCA 
ACATCACATC ACCTTGGCCA GAACTTTTCC AAAATGTTTG AAATTGTCTT TGAAGACCCT 
AAAACTCCTG GGTTAAAACA GTATGCGTAC CAGAACTCAT GGGGTCTTTC AACTCGCACA 
ATAGGAGCAA TGACCATGGT TCATGCAGAC AACATTGGGA TGGTGCTCCC ACCCAGAGTA 
GCCTGTGTGC AGGTAATAAT AATCCCTTGT GGCATCACAA ACACCCTTTC CGAGGAAGAC 
AAAGATGCCC TCCTAAAGAA ATGTGACCAG TATCTGAAGC GCCTTCTCAA CGTTGATGTA 
CGGGCACGCG CAGATGTGAG GGACAACTAC TCCCCGGGAT GGAAGTTCAA TCACTGGGAG 
CTCAAGGGTG TTCCAATCAG GCTTGAGGTT GGGCCACGAG ACATGAAAAA TAAGCAGTTT 
GTAGCAGTCA GAAGAGACAA TGGAGAGAAA CAAACCATCT CAGAGGACCA GGCCGAGACG 
CGGTTAAAGG AAATCTTGGA GGAAATTCAC ACAAATCTAT ATAACAGGGC GCTCAGTGAT 
CTGACAAGTA ACATGGTGGT AGCCAACACG ATGGAAGACT TCCAGAAGAA TTTGGAGAAG 
GGAAGGATTG TACAGATCCC ATTCTGCGGG GAAATTGAAT GTGAAGATTG GATAAAGAAG 
GTAACAGCAA GGGATCAAGA TCTGGAGCCA GGAGCACCTT CCATGGGAGC CAAAAGCCTC 
TGTATCCCCT TCAAACCTCT CAGTGAGCTG CAGCCCAGCG CCAAGTGTGT CTGCGAGAAG 
AACGCCGCCA AGTTCTACAC ACTATTTGGC CGCAGTTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eprs1 ( XM_012963956.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819410.1 Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X3, mRNA.

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CloneID	OXa31527
Clone ID Related Accession (Same CDS sequence)	XM_012963956.1
Accession Version	XM_012963956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bifunctional glutamate/proline--tRNA ligase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACTA GCCCTTCCAG ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATCAGGTAG ATGGAGCTGT GAAAACCCTT 
CTAGCTTTAA AAGCAGAATA TAAACAAGTA ACAGGTCAGG AGTACAAGCC AGGGAGCCCT 
CCAGTAGTGC CAAGCAGCTC TTCTCCACCT CCCGCTAGTC AGAGCTTCTC CAATACCTCA 
GATCTGATAT TGTACAACAG AGTGGTTGAG CAAGGAGAGG TGGTCCGACA GCTCAAAAGC 
AAGAAAGCCC CCAAGGATAA AGTGGATGAG GCTGTGAAAC TGCTACTGGC TCTGAAGGCT 
GAATATAAGG AGAAGACAGG GCAGGAGTAT AAACCAGGAC AGACCCCGGC TGCTGCTCCC 
ACAAAAAAGG CCCTGGAAAA ACCCACAAGT TCTCAGGCAA ATGGCTCACA CAGCGCCGAG 
GCAGAAGAGC TGTATAACAG AGTGGCCAGA CAGGGGGAGG AAGTACGCAA AGTGAAGTCT 
GAGAAAGCCG CAAAGGACAA AGTGGATGCT GCCGTAAAGG AGCTTCTAGA TTTAAAGGCT 
CAATACAAAG CTTTGACGGG CACAGAGTAC AAACCAGTGG CTGCCCCAAG CTCAGAGGAA 
AAAAAGCACA AGGAGAAGGA GAACAAGTCT GAGAAGCAAA GCAAGCAATC AAAGGCTGGA 
GAAGGGCCCA AGAAGAAGGA TACTTCCAAA GATCAAGGGG ACAATTCTGC TGCTGGAGAA 
AGTCAAGGAC CTAAAAAACA GACCAGGTTG GGATTGGAGG CTAAAAAGGA AGAGAACTTA 
CCAGACTGGT ATTCCCAGGT GATCACCAAA TCAGAGATGA TCGAATACTA TGACGTCAGT 
GGCTGTTACG TGCTGCGTCC CTGGGCCTTT GCCATCTGGG AGGCCATTAA AGAGTTCTTT 
GATAAAGAGA TAAAGAAGCT CGGTGTGGAA AACTGTTACT TCCCCATGTT CGTGTCTCAG 
GCTGCGCTGG AGAAGGAAAA AACCCACATT GCAGATTTCG CTCCAGAAGT TGCCTGGGTT 
ACAAGGTCAG GAAAGACAGA GCTAGCAGAA CCAATTGCTG TACGGCCCAC AAGTGAAACT 
GTGATGTATC CCGCTTATGC CAAATGGGTG CAGTCCCACC GAGATCTCCC CATTAAGCTG 
AATCAGTGGT GTAATGTAGT GAGGTGGGAA TTTAAACACC CTCAGCCTTT CCTCCGAACA 
CGTGAGTTCC TTTGGCAAGA AGGACATACA GCGTTCGCTA CATACGAGGA AGCTGCAGAA 
GAGGTCTTGC AGATACTGGA TCTTTATGCC CGTGTCTATG AGGATCTCTT GGCTATTCCT 
GTTGTAAAAG GAAGAAAGAC TGAAAAAGAA AAGTTTGCAG GTGGGGACTA CACAACCACA 
GTGGAAGCAT TTATATCGGC CAGCAGCCGT GCCATACAGG GTGCAACATC ACATCACCTT 
GGCCAGAACT TTTCCAAAAT GTTTGAAATT GTCTTTGAAG ACCCTAAAAC TCCTGGGTTA 
AAACAGTATG CGTACCAGAA CTCATGGGGT CTTTCAACTC GCACAATAGG AGCAATGACC 
ATGGTTCATG CAGACAACAT TGGGATGGTG CTCCCACCCA GAGTAGCCTG TGTGCAGGTA 
ATAATAATCC CTTGTGGCAT CACAAACACC CTTTCCGAGG AAGACAAAGA TGCCCTCCTA 
AAGAAATGTG ACCAGTATCT GAAGCGCCTT CTCAACGTTG ATGTACGGGC ACGCGCAGAT 
GTGAGGGACA ACTACTCCCC GGGATGGAAG TTCAATCACT GGGAGCTCAA GGGTGTTCCA 
ATCAGGCTTG AGGTTGGGCC ACGAGACATG AAAAATAAGC AGTTTGTAGC AGTCAGAAGA 
GACAATGGAG AGAAACAAAC CATCTCAGAG GACCAGGCCG AGACGCGGTT AAAGGAAATC 
TTGGAGGAAA TTCACACAAA TCTATATAAC AGGGCGCTCA GTGATCTGAC AAGTAACATG 
GTGGTAGCCA ACACGATGGA AGACTTCCAG AAGAATTTGG AGAAGGGAAG GATTGTACAG 
ATCCCATTCT GCGGGGAAAT TGAATGTGAA GATTGGATAA AGAAGGTAAC AGCAAGGGAT 
CAAGATCTGG AGCCAGGAGC ACCTTCCATG GGAGCCAAAA GCCTCTGTAT CCCCTTCAAA 
CCTCTCAGTG AGCTGCAGCC CAGCGCCAAG TGTGTCTGCG AGAAGAACGC CGCCAAGTTC 
TACACACTAT TTGGCCGCAG TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819410.1
CDS	42..4928
Translation	MSTSPSRQEVRLLPGQCWSVCEPEAGCTLSHSARSFVAAAGISSSIAGGDSMALTLTVSSSSPSLAVLWTVEHLKGQIQIHVQEGKDTHLRVTDTISFTDVHSIVRYLARLAPDKGLSGSHLTEQTEVSHWMEFSQTKLMQKGEFSTGLQELNHCLSLRTYLVGNALTLADICVFSALEASSEWQALVQKGQTPVNVRRWYSFLEPQLGFWSRTNNKVPSEKKQDVGKFVELPGAEMGKVIVRFPPEASGYLHIGHAKAALLNQHYQVTFKGKLIMRFDDTNPEKEKEDFEKVILEDVSMLQIKPDQFTYTSDHFDTIMKYAEKLIQEGKAYVDDTPAEQMKSEREQRIESKHRSNTVAKNMEMWEDMKKGTEYGQTCCLRAKIDMNSNNGCMRDPTLFRCKNQPHPRTGSKYNIYPTYDFACPIVDSVEGVTHALRTTEYHDRDEQFYWIIDALGIRKPYIWEYSRLNLNNTVLSKRRLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYSALLKSQAVAVNIPDAKEGMVEVAKHPKNAEVGQKPVWYGPKVLIEGADAETLSEGETVTFINWGNLIITKIHRDQSGKIQSLDAKLNLENKDYKKTTKITWLAETPKAPFIPTSCVNYDHLITKPVLGKEEDFKQYVNRSSKQEEVMLGDPCLKDLKKGDLIQLQRRGFYICDEPYEPISPHSCKEAPCVLIYIPDGHTKEMPTSGSKEKSKTEATKKETQPKSKGKAANVPVSVAPLASPEEDPVALYNKVSAQGDLVRDLKSKKAAKAEVDEAVKLLLSLKAQYKEVTGQEYKPGSAPAQKPAPSPAASSSGDAKAIYEKVSAQGETVRKLKTEKAPKDQVDGAVKTLLALKAEYKQVTGQEYKPGSPPVVPSSSSPPPASQSFSNTSDLILYNRVVEQGEVVRQLKSKKAPKDKVDEAVKLLLALKAEYKEKTGQEYKPGQTPAAAPTKKALEKPTSSQANGSHSAEAEELYNRVARQGEEVRKVKSEKAAKDKVDAAVKELLDLKAQYKALTGTEYKPVAAPSSEEKKHKEKENKSEKQSKQSKAGEGPKKKDTSKDQGDNSAAGESQGPKKQTRLGLEAKKEENLPDWYSQVITKSEMIEYYDVSGCYVLRPWAFAIWEAIKEFFDKEIKKLGVENCYFPMFVSQAALEKEKTHIADFAPEVAWVTRSGKTELAEPIAVRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHTAFATYEEAAEEVLQILDLYARVYEDLLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASSRAIQGATSHHLGQNFSKMFEIVFEDPKTPGLKQYAYQNSWGLSTRTIGAMTMVHADNIGMVLPPRVACVQVIIIPCGITNTLSEEDKDALLKKCDQYLKRLLNVDVRARADVRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNKQFVAVRRDNGEKQTISEDQAETRLKEILEEIHTNLYNRALSDLTSNMVVANTMEDFQKNLEKGRIVQIPFCGEIECEDWIKKVTARDQDLEPGAPSMGAKSLCIPFKPLSELQPSAKCVCEKNAAKFYTLFGRSY

Target ORF information:

RefSeq Version	XM_012963956.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamyl-prolyl-tRNA synthetase (eprs), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963956.1

ORF Insert Sequence:

ATGTCTACTA GCCCTTCCAG ACAGGAAGTG AGGTTATTGC CAGGGCAGTG CTGGAGCGTG 
TGTGAGCCGG AAGCGGGCTG TACTCTGTCT CATTCAGCCA GGAGCTTCGT GGCAGCCGCT 
GGGATAAGCA GTAGCATCGC TGGCGGAGAC AGCATGGCGC TTACCTTGAC CGTGTCGAGC 
TCCAGCCCCT CTCTGGCTGT CCTCTGGACC GTGGAACACC TAAAGGGCCA AATACAGATC 
CATGTGCAGG AAGGGAAGGA CACCCACCTC CGGGTAACAG ACACTATCTC CTTCACTGAT 
GTGCACTCTA TCGTACGCTA TCTGGCCAGA CTTGCACCGG ATAAAGGACT GAGTGGGTCA 
CATCTCACAG AGCAGACAGA GGTGAGCCAT TGGATGGAGT TTAGTCAGAC CAAACTGATG 
CAAAAGGGCG AGTTTTCCAC AGGCCTCCAG GAGCTCAATC ACTGCCTGTC TCTGCGCACG 
TACTTGGTGG GGAACGCCCT CACCCTGGCT GATATCTGTG TCTTCTCTGC CTTGGAAGCA 
AGCAGTGAAT GGCAGGCACT GGTGCAGAAG GGCCAGACCC CAGTAAATGT CAGAAGGTGG 
TACAGCTTTC TAGAACCCCA GCTTGGTTTC TGGAGCAGAA CAAACAACAA AGTGCCCAGT 
GAAAAAAAAC AAGATGTTGG AAAGTTTGTA GAGCTTCCAG GTGCAGAGAT GGGCAAGGTA 
ATCGTGAGAT TCCCTCCTGA GGCCAGCGGC TACTTGCACA TTGGCCATGC CAAAGCCGCG 
CTCCTCAACC AGCACTATCA GGTCACCTTC AAAGGAAAAC TTATCATGAG GTTTGATGAC 
ACCAACCCAG AAAAGGAGAA AGAAGACTTT GAGAAGGTTA TACTGGAAGA TGTTTCCATG 
CTGCAGATCA AGCCGGATCA GTTTACGTAC ACCTCCGATC ACTTTGACAC CATCATGAAA 
TACGCAGAGA AGCTGATCCA GGAGGGAAAG GCCTATGTGG ATGACACCCC GGCAGAACAA 
ATGAAATCAG AGCGAGAGCA GAGAATAGAG TCCAAGCACA GGAGTAACAC CGTGGCAAAG 
AACATGGAAA TGTGGGAAGA TATGAAAAAA GGCACAGAAT ACGGTCAGAC CTGCTGTCTC 
CGAGCTAAAA TCGACATGAA TTCCAACAAT GGCTGCATGA GGGACCCCAC TTTGTTCCGC 
TGCAAGAATC AACCCCACCC CCGGACAGGA AGCAAATACA ATATCTATCC CACGTATGAC 
TTTGCCTGCC CCATTGTGGA CAGTGTGGAG GGTGTCACTC ACGCTTTGCG CACAACAGAG 
TATCACGACC GGGACGAGCA GTTCTACTGG ATTATCGATG CCCTGGGCAT CCGGAAGCCA 
TACATCTGGG AATACAGTCG CCTGAACCTC AACAATACCG TCCTCTCCAA GAGAAGGCTG 
ACCTGGTTTG TCAATGAAGG CCTAGTGGAT GGCTGGGATG ACCCAAGGTT CCCTACAGTG 
CGTGGGGTTC TCAGAAGAGG AATGACAGTG GAGGGGCTGA AACAGTTCAT TGCAGCGCAG 
GGCTCTTCAC GATCAGTGGT AAACATGGAG TGGGACAAGA TCTGGGCATT TAACAAAAAG 
GTGATTGATC CCGTGGCCCC CCGATACTCC GCCTTGCTGA AGAGCCAGGC CGTCGCTGTG 
AACATTCCCG ACGCTAAAGA GGGGATGGTG GAAGTGGCCA AGCACCCAAA GAACGCAGAG 
GTGGGCCAGA AGCCTGTGTG GTACGGCCCC AAGGTCCTCA TAGAAGGTGC AGATGCAGAA 
ACCTTGTCAG AGGGGGAGAC AGTCACATTC ATAAACTGGG GAAATCTCAT CATTACAAAG 
ATTCACCGGG ACCAGAGTGG GAAAATCCAG TCCCTCGATG CCAAGCTTAA TCTGGAAAAC 
AAAGATTACA AGAAAACCAC TAAAATCACC TGGCTGGCCG AAACCCCCAA GGCACCTTTT 
ATCCCAACAT CCTGTGTTAA TTACGATCAC CTTATTACCA AGCCAGTTTT GGGCAAGGAG 
GAAGACTTCA AGCAGTATGT TAACAGAAGC AGCAAGCAAG AAGAAGTGAT GTTGGGTGAC 
CCCTGCCTGA AAGATCTTAA AAAGGGAGAC CTCATCCAGC TTCAGAGGAG AGGCTTCTAC 
ATCTGTGATG AGCCTTATGA GCCAATCAGC CCTCACAGCT GTAAGGAGGC CCCATGTGTT 
CTGATTTACA TCCCAGATGG ACACACCAAG GAGATGCCAA CATCAGGCTC AAAGGAGAAG 
TCCAAGACTG AGGCTACCAA GAAAGAGACC CAGCCCAAAT CGAAGGGTAA AGCTGCAAAT 
GTGCCAGTAA GTGTGGCTCC TCTAGCATCT CCCGAAGAGG ATCCTGTGGC TCTGTACAAC 
AAGGTTTCTG CACAGGGAGA TCTAGTAAGG GACCTGAAAT CAAAGAAAGC TGCAAAAGCG 
GAAGTCGATG AAGCAGTTAA GCTCCTTTTG TCTCTGAAAG CACAATACAA AGAAGTCACA 
GGGCAGGAAT ACAAACCGGG CAGTGCCCCG GCACAGAAAC CTGCTCCCAG CCCAGCAGCC 
AGCAGCAGTG GTGATGCAAA AGCCATTTAC GAGAAAGTGT CTGCACAAGG GGAGACGGTC 
CGGAAACTAA AGACAGAGAA GGCACCAAAG GATCAGGTAG ATGGAGCTGT GAAAACCCTT 
CTAGCTTTAA AAGCAGAATA TAAACAAGTA ACAGGTCAGG AGTACAAGCC AGGGAGCCCT 
CCAGTAGTGC CAAGCAGCTC TTCTCCACCT CCCGCTAGTC AGAGCTTCTC CAATACCTCA 
GATCTGATAT TGTACAACAG AGTGGTTGAG CAAGGAGAGG TGGTCCGACA GCTCAAAAGC 
AAGAAAGCCC CCAAGGATAA AGTGGATGAG GCTGTGAAAC TGCTACTGGC TCTGAAGGCT 
GAATATAAGG AGAAGACAGG GCAGGAGTAT AAACCAGGAC AGACCCCGGC TGCTGCTCCC 
ACAAAAAAGG CCCTGGAAAA ACCCACAAGT TCTCAGGCAA ATGGCTCACA CAGCGCCGAG 
GCAGAAGAGC TGTATAACAG AGTGGCCAGA CAGGGGGAGG AAGTACGCAA AGTGAAGTCT 
GAGAAAGCCG CAAAGGACAA AGTGGATGCT GCCGTAAAGG AGCTTCTAGA TTTAAAGGCT 
CAATACAAAG CTTTGACGGG CACAGAGTAC AAACCAGTGG CTGCCCCAAG CTCAGAGGAA 
AAAAAGCACA AGGAGAAGGA GAACAAGTCT GAGAAGCAAA GCAAGCAATC AAAGGCTGGA 
GAAGGGCCCA AGAAGAAGGA TACTTCCAAA GATCAAGGGG ACAATTCTGC TGCTGGAGAA 
AGTCAAGGAC CTAAAAAACA GACCAGGTTG GGATTGGAGG CTAAAAAGGA AGAGAACTTA 
CCAGACTGGT ATTCCCAGGT GATCACCAAA TCAGAGATGA TCGAATACTA TGACGTCAGT 
GGCTGTTACG TGCTGCGTCC CTGGGCCTTT GCCATCTGGG AGGCCATTAA AGAGTTCTTT 
GATAAAGAGA TAAAGAAGCT CGGTGTGGAA AACTGTTACT TCCCCATGTT CGTGTCTCAG 
GCTGCGCTGG AGAAGGAAAA AACCCACATT GCAGATTTCG CTCCAGAAGT TGCCTGGGTT 
ACAAGGTCAG GAAAGACAGA GCTAGCAGAA CCAATTGCTG TACGGCCCAC AAGTGAAACT 
GTGATGTATC CCGCTTATGC CAAATGGGTG CAGTCCCACC GAGATCTCCC CATTAAGCTG 
AATCAGTGGT GTAATGTAGT GAGGTGGGAA TTTAAACACC CTCAGCCTTT CCTCCGAACA 
CGTGAGTTCC TTTGGCAAGA AGGACATACA GCGTTCGCTA CATACGAGGA AGCTGCAGAA 
GAGGTCTTGC AGATACTGGA TCTTTATGCC CGTGTCTATG AGGATCTCTT GGCTATTCCT 
GTTGTAAAAG GAAGAAAGAC TGAAAAAGAA AAGTTTGCAG GTGGGGACTA CACAACCACA 
GTGGAAGCAT TTATATCGGC CAGCAGCCGT GCCATACAGG GTGCAACATC ACATCACCTT 
GGCCAGAACT TTTCCAAAAT GTTTGAAATT GTCTTTGAAG ACCCTAAAAC TCCTGGGTTA 
AAACAGTATG CGTACCAGAA CTCATGGGGT CTTTCAACTC GCACAATAGG AGCAATGACC 
ATGGTTCATG CAGACAACAT TGGGATGGTG CTCCCACCCA GAGTAGCCTG TGTGCAGGTA 
ATAATAATCC CTTGTGGCAT CACAAACACC CTTTCCGAGG AAGACAAAGA TGCCCTCCTA 
AAGAAATGTG ACCAGTATCT GAAGCGCCTT CTCAACGTTG ATGTACGGGC ACGCGCAGAT 
GTGAGGGACA ACTACTCCCC GGGATGGAAG TTCAATCACT GGGAGCTCAA GGGTGTTCCA 
ATCAGGCTTG AGGTTGGGCC ACGAGACATG AAAAATAAGC AGTTTGTAGC AGTCAGAAGA 
GACAATGGAG AGAAACAAAC CATCTCAGAG GACCAGGCCG AGACGCGGTT AAAGGAAATC 
TTGGAGGAAA TTCACACAAA TCTATATAAC AGGGCGCTCA GTGATCTGAC AAGTAACATG 
GTGGTAGCCA ACACGATGGA AGACTTCCAG AAGAATTTGG AGAAGGGAAG GATTGTACAG 
ATCCCATTCT GCGGGGAAAT TGAATGTGAA GATTGGATAA AGAAGGTAAC AGCAAGGGAT 
CAAGATCTGG AGCCAGGAGC ACCTTCCATG GGAGCCAAAA GCCTCTGTAT CCCCTTCAAA 
CCTCTCAGTG AGCTGCAGCC CAGCGCCAAG TGTGTCTGCG AGAAGAACGC CGCCAAGTTC 
TACACACTAT TTGGCCGCAG TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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eprs1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

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