ift140 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ift140
Entrez Gene ID	100144437
Full Name	intraflagellar transport 140
Synonyms	c305c8.4,c380f5.1,gs114,wdtc2
General protein information	Preferred Names intraflagellar transport 140 Names intraflagellar transport protein 140 homolog intraflagellar transport 140 homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012970030.2	XP_012825484.1	intraflagellar transport protein 140 homolog isoform X1
XM_012970031.2	XP_012825485.1	intraflagellar transport protein 140 homolog isoform X1
XM_012970029.2	XP_012825483.1	intraflagellar transport protein 140 homolog isoform X1
XM_031892490.1	XP_031748350.1	intraflagellar transport protein 140 homolog isoform X1
XM_031892491.1	XP_031748351.1	intraflagellar transport protein 140 homolog isoform X1
XM_018097033.1	XP_017952522.1	intraflagellar transport protein 140 homolog isoform X1
XM_031892492.1	XP_031748352.1	intraflagellar transport protein 140 homolog isoform X1
NM_001123025.1	NP_001116497.1	intraflagellar transport protein 140 homolog

Pathways from BioSystems

Homologies

	IFT140	XP_001089057.1
Bos taurus (cattle)	IFT140	XP_002697959.3
Gallus gallus (chicken)	IFT140	NP_001012810.1
Danio rerio (zebrafish)	ift140	XP_695732.4
Mus musculus (house mouse)	Ift140	NP_598887.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000686	XP_311215.4
Pan troglodytes (chimpanzee)	IFT140	XP_003314958.1
Caenorhabditis elegans (roundworm)	che-11	NP_506047.5
Xenopus tropicalis (tropical clawed frog)	ift140	NP_001116497.1
Homo sapiens (human)	IFT140	NP_055529.2
Rattus norvegicus (Norway rat)	Ift140	XP_006246116.1
Drosophila melanogaster (fruit fly)	rempA	NP_995608.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ift140 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ift140 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa65845	XM_031892492.1 Latest version!	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa65845	XM_031892490.1 Latest version!	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa65845	XM_031892491.1 Latest version!	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa21872	NM_001123025.1 Latest version!	Xenopus tropicalis intraflagellar transport 140 (ift140), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa31338	XM_012970029.2 Latest version!	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa31338	XM_012970031.2 Latest version!	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa31338	XM_012970030.2 Latest version!	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa31338	XM_018097033.1 Latest version!	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ift140 ( XM_031892490.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748350.1 Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa65845
Clone ID Related Accession (Same CDS sequence)	XM_031892490.1 , XM_031892491.1 , XM_031892492.1
Accession Version	XM_031892490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAA TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTACCAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA CATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGC GAGGTGAGGA GGAGAAAGCC 
TCTGAAACAG AGGTGCAGGC TCCGCAGATT CCCCAGATGG TGGCAAAGAG ACCCCTGAGA 
GACTTTATTG GGCTGGAAGA ATGCGATAAG ACCACAAGAG AAGCCATGCT CAACTTCAGC 
TTTTACCTCA CAGTGGGAGA TATGGATGAA GCATTTAAGT CTATCAAGCT TATTAAAAGT 
GAAGCAGTCT GGGAAAACAT GGCTCAAATG TGCGTTAAAA CCAAGAGGCT GGATGTCGCC 
AAGGTTTGCC TAGGAAATAT GGGCCATGCC AGGGGTGCCA AGGCCTTACG AGAGGCAGAA 
CAGGAGCCAG AGGTGGAAGC GAGAGTGGCC ATGCTGGCAA TACAGCTGGG AATGTTGGAA 
GAAGCCGAAC AACTGTACAA AAGTTGCCAG CGATACGACC TCCTGAATAA GCTTTACCAA 
GCTTGGAACC AGTGGCAGAA GGCTATTGAA ACTGCAGAAA ACCATGACAG AATCCACCTG 
CGCTCCACCT ACTACAACTA CGCCAAGCAC CTAGAGGCCA TTGGGGAACA CAATGAGTCA 
CTCCTCTTGT ATGAGAAATC TGATACTCAC CGGTTTGAGG TTCCCAGAAT GCTCCTGGAT 
GACCCCCAGG CTTTGGAAGA GTACATCAAT AAAAATAAGG ATAAGGGACT GTGGAAGTGG 
TGGGCACAAT ATTTGGAAAG CCAATCAAAC CTAGACGCTG CGCTCAAATA CTATGAGATG 
GCTCAGGACT ACCTGTCACT GGTCCGGGTG CACTGCTTCC TGGGGAATAT CCAAAGGGCA 
GCAGAAATAG CGAATGAGAC AGGTAATGGG GCTGCATCCT ACCACCTTGC GCGTCATTAT 
GAGAGCCAGG AAGAGGTGAA GCAAGCGGTG CACTTCTATA CACGTGCCCA GGCATATAAC 
AATGGCATCA GGCTCTGCAA GGAGAACAAC ATGGATGATC AGCTTATGAA CCTTGCTCTG 
CTCAGCTCCC CTGAGGACAT GATGGAGGCA GCTCGGTACT ACGAAGAGAA GGGAGAGCAA 
ATGGATAGGG CAGTCATGCT CTACCACAAG GCAGGACACT TCTCCAAGGC CTTGGAATTG 
GCTTTCACGA CAGAGCAATT TGGGGCTTTG CAGCTGATTG CTGAGGATCT GGATGAAAAG 
TCTGACCCAG CCCTCCTTGC CCGTTGCTCT GACTTTTTCA TGCAGCACAG CCAGTTTGAA 
AAGGCAGTGG AACTCCTGTT GGCAGCCAAG AAGTATTATG AGGCACTACA GCTCTGTATG 
GAACAAAACC TTGTCATTAC AGAAGAAATG GCTGAAAAAA TGACAGTGTC CAAAAATTCC 
AAGGATTTTA GCGACGAGGC CCGGCGTGAA TTGCTGCAAC GCATTGCAGA CTGCTGCATG 
AGGCAAGGGA ACTATCATCT TGCAACCAAG AAATACACTC AGGCAGGGGA TAAGTTAAAG 
GCTATGAAGT CTTTGCTTAA GTCTGGCGAC ACAGAGAAGA TTGTTTTCTT TGCTGGGGTG 
TCACGTCAGA AAGAAATTTA TATTATGGCA GCCAATTACC TGCAGTCCCT GGACTGGCGT 
AAAGATCCTG AGATTATGAA GAACATCATC AGTTTCTACA CTAAAGGCAG AGCCCTGGAA 
CTGTTAGCTG GGTTTTATGA TGCCTGTGCT CAGGTGGAGA TTGATGAATA TCAGAGCTAC 
GAAAAGGCGT TAGGAGCATT TACTGAGGCC TACAAGTGCT TGTCCAAAGC CAAAATGCGA 
AGCCCAGAGG AGCAGGAGAG GAGGCTGTCT GACATGCAAG CCAAGATGAC TCTGGTGAAG 
AGGTTTATCC AGGCTCGCAG GGCATACTCA GTGGACAGTC AAGAAGCAGT AAAGCAATGT 
GAGCTGTTGC TAGAGGAGCC GGATCTAGAC AGCGCAATAC GCATCGGGGA TGTGTATGGG 
CTACTGGTGG AGCACTATAC TCAGCAGGGG GATTTTCAGA AGGCTTACCA GTGCCTTGAG 
GAAATGCGCT CCAAGATGCC ATCTGTCAAC CTGACTTACT ACGTGCCCCA AGGTACTATA 
GAAGCTGTTC ACCGTGCCCT CTCTATTCCA TTTAACCGCC ATTCGCTGTC GGAGCACATG 
AGGCACAATA GCGTGGAGGA TAGCGAGGAG GTGGAGGAAG TACCGGAGAT GGACTATGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748350.1
CDS	366..4751
Translation	MAVYFDHRIQAPFPSASPSHISWHKHHALLAVGYISSGDGGSVDIYNELGDHVQDAQIKRTSHPTVLSWHPTKRTLVTGWTTGELVVFNEQDKEEYTLQTTHTAEITVLEWSSGTSRLVSADMSGTVAVWRLDQRGRIQVSPLLTNMYHKPLTHCLFKPLAPDEDLAQLAKAAVSGDEKALDMFNWNKSGKWTPMKSVSQQEFHFFLSAKDGSVYFVNEQLKNSQIVTLDSPVKRLFFMEKKSILVIVTENLLLSQFFISQEGNAEEISKVKLSGKTGQAADIHWVDNSLLITATGENVIRLWDLEREDNYVLSLDDQLGYDFAETLNCISYCSAKAILAAGTNKGRVAMWSRVTSHGQKAIRTDGKGTWKLQTPTELEENIMQIQWGSSKNFLAINNFSSVVVLSEQVMSAHFSQQVAAIQVAPTQLNLTYFSTETTHNLQTDMYIKGVFVTKDTIAVWNGKMVTVYELSGPTLRNTGSFSCESPVLVMHEDNIYTVETNRVQIWTLQGTVKQLLPFSEAEGNPCGLDICGHFLAVATDTAHFKIFDLSRREAKAHINCKNLSQMVPGVGSIVSVKCNVNGSKVSILANRSDRNLDSRMYFYDAELDTVTFYDFWSGQAEQGDSISNNDPDEHDAENRVLLDRIPINHFWDQSEPRLVVCEAMLAVPKSANTSPKHQSDQQTEIHQGKEDILVISLFSTQEHGLLIQESFPRPAALQSLLGIEVPHYYFTKKRGEEEKASETEVQAPQIPQMVAKRPLRDFIGLEECDKTTREAMLNFSFYLTVGDMDEAFKSIKLIKSEAVWENMAQMCVKTKRLDVAKVCLGNMGHARGAKALREAEQEPEVEARVAMLAIQLGMLEEAEQLYKSCQRYDLLNKLYQAWNQWQKAIETAENHDRIHLRSTYYNYAKHLEAIGEHNESLLLYEKSDTHRFEVPRMLLDDPQALEEYINKNKDKGLWKWWAQYLESQSNLDAALKYYEMAQDYLSLVRVHCFLGNIQRAAEIANETGNGAASYHLARHYESQEEVKQAVHFYTRAQAYNNGIRLCKENNMDDQLMNLALLSSPEDMMEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTEQFGALQLIAEDLDEKSDPALLARCSDFFMQHSQFEKAVELLLAAKKYYEALQLCMEQNLVITEEMAEKMTVSKNSKDFSDEARRELLQRIADCCMRQGNYHLATKKYTQAGDKLKAMKSLLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKDPEIMKNIISFYTKGRALELLAGFYDACAQVEIDEYQSYEKALGAFTEAYKCLSKAKMRSPEEQERRLSDMQAKMTLVKRFIQARRAYSVDSQEAVKQCELLLEEPDLDSAIRIGDVYGLLVEHYTQQGDFQKAYQCLEEMRSKMPSVNLTYYVPQGTIEAVHRALSIPFNRHSLSEHMRHNSVEDSEEVEEVPEMDYGD

Target ORF information:

RefSeq Version	XM_031892490.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892490.1

ORF Insert Sequence:

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAA TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTACCAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA CATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGC GAGGTGAGGA GGAGAAAGCC 
TCTGAAACAG AGGTGCAGGC TCCGCAGATT CCCCAGATGG TGGCAAAGAG ACCCCTGAGA 
GACTTTATTG GGCTGGAAGA ATGCGATAAG ACCACAAGAG AAGCCATGCT CAACTTCAGC 
TTTTACCTCA CAGTGGGAGA TATGGATGAA GCATTTAAGT CTATCAAGCT TATTAAAAGT 
GAAGCAGTCT GGGAAAACAT GGCTCAAATG TGCGTTAAAA CCAAGAGGCT GGATGTCGCC 
AAGGTTTGCC TAGGAAATAT GGGCCATGCC AGGGGTGCCA AGGCCTTACG AGAGGCAGAA 
CAGGAGCCAG AGGTGGAAGC GAGAGTGGCC ATGCTGGCAA TACAGCTGGG AATGTTGGAA 
GAAGCCGAAC AACTGTACAA AAGTTGCCAG CGATACGACC TCCTGAATAA GCTTTACCAA 
GCTTGGAACC AGTGGCAGAA GGCTATTGAA ACTGCAGAAA ACCATGACAG AATCCACCTG 
CGCTCCACCT ACTACAACTA CGCCAAGCAC CTAGAGGCCA TTGGGGAACA CAATGAGTCA 
CTCCTCTTGT ATGAGAAATC TGATACTCAC CGGTTTGAGG TTCCCAGAAT GCTCCTGGAT 
GACCCCCAGG CTTTGGAAGA GTACATCAAT AAAAATAAGG ATAAGGGACT GTGGAAGTGG 
TGGGCACAAT ATTTGGAAAG CCAATCAAAC CTAGACGCTG CGCTCAAATA CTATGAGATG 
GCTCAGGACT ACCTGTCACT GGTCCGGGTG CACTGCTTCC TGGGGAATAT CCAAAGGGCA 
GCAGAAATAG CGAATGAGAC AGGTAATGGG GCTGCATCCT ACCACCTTGC GCGTCATTAT 
GAGAGCCAGG AAGAGGTGAA GCAAGCGGTG CACTTCTATA CACGTGCCCA GGCATATAAC 
AATGGCATCA GGCTCTGCAA GGAGAACAAC ATGGATGATC AGCTTATGAA CCTTGCTCTG 
CTCAGCTCCC CTGAGGACAT GATGGAGGCA GCTCGGTACT ACGAAGAGAA GGGAGAGCAA 
ATGGATAGGG CAGTCATGCT CTACCACAAG GCAGGACACT TCTCCAAGGC CTTGGAATTG 
GCTTTCACGA CAGAGCAATT TGGGGCTTTG CAGCTGATTG CTGAGGATCT GGATGAAAAG 
TCTGACCCAG CCCTCCTTGC CCGTTGCTCT GACTTTTTCA TGCAGCACAG CCAGTTTGAA 
AAGGCAGTGG AACTCCTGTT GGCAGCCAAG AAGTATTATG AGGCACTACA GCTCTGTATG 
GAACAAAACC TTGTCATTAC AGAAGAAATG GCTGAAAAAA TGACAGTGTC CAAAAATTCC 
AAGGATTTTA GCGACGAGGC CCGGCGTGAA TTGCTGCAAC GCATTGCAGA CTGCTGCATG 
AGGCAAGGGA ACTATCATCT TGCAACCAAG AAATACACTC AGGCAGGGGA TAAGTTAAAG 
GCTATGAAGT CTTTGCTTAA GTCTGGCGAC ACAGAGAAGA TTGTTTTCTT TGCTGGGGTG 
TCACGTCAGA AAGAAATTTA TATTATGGCA GCCAATTACC TGCAGTCCCT GGACTGGCGT 
AAAGATCCTG AGATTATGAA GAACATCATC AGTTTCTACA CTAAAGGCAG AGCCCTGGAA 
CTGTTAGCTG GGTTTTATGA TGCCTGTGCT CAGGTGGAGA TTGATGAATA TCAGAGCTAC 
GAAAAGGCGT TAGGAGCATT TACTGAGGCC TACAAGTGCT TGTCCAAAGC CAAAATGCGA 
AGCCCAGAGG AGCAGGAGAG GAGGCTGTCT GACATGCAAG CCAAGATGAC TCTGGTGAAG 
AGGTTTATCC AGGCTCGCAG GGCATACTCA GTGGACAGTC AAGAAGCAGT AAAGCAATGT 
GAGCTGTTGC TAGAGGAGCC GGATCTAGAC AGCGCAATAC GCATCGGGGA TGTGTATGGG 
CTACTGGTGG AGCACTATAC TCAGCAGGGG GATTTTCAGA AGGCTTACCA GTGCCTTGAG 
GAAATGCGCT CCAAGATGCC ATCTGTCAAC CTGACTTACT ACGTGCCCCA AGGTACTATA 
GAAGCTGTTC ACCGTGCCCT CTCTATTCCA TTTAACCGCC ATTCGCTGTC GGAGCACATG 
AGGCACAATA GCGTGGAGGA TAGCGAGGAG GTGGAGGAAG TACCGGAGAT GGACTATGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ift140 ( XM_031892491.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748351.1 Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa65845
Clone ID Related Accession (Same CDS sequence)	XM_031892490.1 , XM_031892491.1 , XM_031892492.1
Accession Version	XM_031892491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAA TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTACCAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA CATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGC GAGGTGAGGA GGAGAAAGCC 
TCTGAAACAG AGGTGCAGGC TCCGCAGATT CCCCAGATGG TGGCAAAGAG ACCCCTGAGA 
GACTTTATTG GGCTGGAAGA ATGCGATAAG ACCACAAGAG AAGCCATGCT CAACTTCAGC 
TTTTACCTCA CAGTGGGAGA TATGGATGAA GCATTTAAGT CTATCAAGCT TATTAAAAGT 
GAAGCAGTCT GGGAAAACAT GGCTCAAATG TGCGTTAAAA CCAAGAGGCT GGATGTCGCC 
AAGGTTTGCC TAGGAAATAT GGGCCATGCC AGGGGTGCCA AGGCCTTACG AGAGGCAGAA 
CAGGAGCCAG AGGTGGAAGC GAGAGTGGCC ATGCTGGCAA TACAGCTGGG AATGTTGGAA 
GAAGCCGAAC AACTGTACAA AAGTTGCCAG CGATACGACC TCCTGAATAA GCTTTACCAA 
GCTTGGAACC AGTGGCAGAA GGCTATTGAA ACTGCAGAAA ACCATGACAG AATCCACCTG 
CGCTCCACCT ACTACAACTA CGCCAAGCAC CTAGAGGCCA TTGGGGAACA CAATGAGTCA 
CTCCTCTTGT ATGAGAAATC TGATACTCAC CGGTTTGAGG TTCCCAGAAT GCTCCTGGAT 
GACCCCCAGG CTTTGGAAGA GTACATCAAT AAAAATAAGG ATAAGGGACT GTGGAAGTGG 
TGGGCACAAT ATTTGGAAAG CCAATCAAAC CTAGACGCTG CGCTCAAATA CTATGAGATG 
GCTCAGGACT ACCTGTCACT GGTCCGGGTG CACTGCTTCC TGGGGAATAT CCAAAGGGCA 
GCAGAAATAG CGAATGAGAC AGGTAATGGG GCTGCATCCT ACCACCTTGC GCGTCATTAT 
GAGAGCCAGG AAGAGGTGAA GCAAGCGGTG CACTTCTATA CACGTGCCCA GGCATATAAC 
AATGGCATCA GGCTCTGCAA GGAGAACAAC ATGGATGATC AGCTTATGAA CCTTGCTCTG 
CTCAGCTCCC CTGAGGACAT GATGGAGGCA GCTCGGTACT ACGAAGAGAA GGGAGAGCAA 
ATGGATAGGG CAGTCATGCT CTACCACAAG GCAGGACACT TCTCCAAGGC CTTGGAATTG 
GCTTTCACGA CAGAGCAATT TGGGGCTTTG CAGCTGATTG CTGAGGATCT GGATGAAAAG 
TCTGACCCAG CCCTCCTTGC CCGTTGCTCT GACTTTTTCA TGCAGCACAG CCAGTTTGAA 
AAGGCAGTGG AACTCCTGTT GGCAGCCAAG AAGTATTATG AGGCACTACA GCTCTGTATG 
GAACAAAACC TTGTCATTAC AGAAGAAATG GCTGAAAAAA TGACAGTGTC CAAAAATTCC 
AAGGATTTTA GCGACGAGGC CCGGCGTGAA TTGCTGCAAC GCATTGCAGA CTGCTGCATG 
AGGCAAGGGA ACTATCATCT TGCAACCAAG AAATACACTC AGGCAGGGGA TAAGTTAAAG 
GCTATGAAGT CTTTGCTTAA GTCTGGCGAC ACAGAGAAGA TTGTTTTCTT TGCTGGGGTG 
TCACGTCAGA AAGAAATTTA TATTATGGCA GCCAATTACC TGCAGTCCCT GGACTGGCGT 
AAAGATCCTG AGATTATGAA GAACATCATC AGTTTCTACA CTAAAGGCAG AGCCCTGGAA 
CTGTTAGCTG GGTTTTATGA TGCCTGTGCT CAGGTGGAGA TTGATGAATA TCAGAGCTAC 
GAAAAGGCGT TAGGAGCATT TACTGAGGCC TACAAGTGCT TGTCCAAAGC CAAAATGCGA 
AGCCCAGAGG AGCAGGAGAG GAGGCTGTCT GACATGCAAG CCAAGATGAC TCTGGTGAAG 
AGGTTTATCC AGGCTCGCAG GGCATACTCA GTGGACAGTC AAGAAGCAGT AAAGCAATGT 
GAGCTGTTGC TAGAGGAGCC GGATCTAGAC AGCGCAATAC GCATCGGGGA TGTGTATGGG 
CTACTGGTGG AGCACTATAC TCAGCAGGGG GATTTTCAGA AGGCTTACCA GTGCCTTGAG 
GAAATGCGCT CCAAGATGCC ATCTGTCAAC CTGACTTACT ACGTGCCCCA AGGTACTATA 
GAAGCTGTTC ACCGTGCCCT CTCTATTCCA TTTAACCGCC ATTCGCTGTC GGAGCACATG 
AGGCACAATA GCGTGGAGGA TAGCGAGGAG GTGGAGGAAG TACCGGAGAT GGACTATGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748351.1
CDS	489..4874
Translation	MAVYFDHRIQAPFPSASPSHISWHKHHALLAVGYISSGDGGSVDIYNELGDHVQDAQIKRTSHPTVLSWHPTKRTLVTGWTTGELVVFNEQDKEEYTLQTTHTAEITVLEWSSGTSRLVSADMSGTVAVWRLDQRGRIQVSPLLTNMYHKPLTHCLFKPLAPDEDLAQLAKAAVSGDEKALDMFNWNKSGKWTPMKSVSQQEFHFFLSAKDGSVYFVNEQLKNSQIVTLDSPVKRLFFMEKKSILVIVTENLLLSQFFISQEGNAEEISKVKLSGKTGQAADIHWVDNSLLITATGENVIRLWDLEREDNYVLSLDDQLGYDFAETLNCISYCSAKAILAAGTNKGRVAMWSRVTSHGQKAIRTDGKGTWKLQTPTELEENIMQIQWGSSKNFLAINNFSSVVVLSEQVMSAHFSQQVAAIQVAPTQLNLTYFSTETTHNLQTDMYIKGVFVTKDTIAVWNGKMVTVYELSGPTLRNTGSFSCESPVLVMHEDNIYTVETNRVQIWTLQGTVKQLLPFSEAEGNPCGLDICGHFLAVATDTAHFKIFDLSRREAKAHINCKNLSQMVPGVGSIVSVKCNVNGSKVSILANRSDRNLDSRMYFYDAELDTVTFYDFWSGQAEQGDSISNNDPDEHDAENRVLLDRIPINHFWDQSEPRLVVCEAMLAVPKSANTSPKHQSDQQTEIHQGKEDILVISLFSTQEHGLLIQESFPRPAALQSLLGIEVPHYYFTKKRGEEEKASETEVQAPQIPQMVAKRPLRDFIGLEECDKTTREAMLNFSFYLTVGDMDEAFKSIKLIKSEAVWENMAQMCVKTKRLDVAKVCLGNMGHARGAKALREAEQEPEVEARVAMLAIQLGMLEEAEQLYKSCQRYDLLNKLYQAWNQWQKAIETAENHDRIHLRSTYYNYAKHLEAIGEHNESLLLYEKSDTHRFEVPRMLLDDPQALEEYINKNKDKGLWKWWAQYLESQSNLDAALKYYEMAQDYLSLVRVHCFLGNIQRAAEIANETGNGAASYHLARHYESQEEVKQAVHFYTRAQAYNNGIRLCKENNMDDQLMNLALLSSPEDMMEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTEQFGALQLIAEDLDEKSDPALLARCSDFFMQHSQFEKAVELLLAAKKYYEALQLCMEQNLVITEEMAEKMTVSKNSKDFSDEARRELLQRIADCCMRQGNYHLATKKYTQAGDKLKAMKSLLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKDPEIMKNIISFYTKGRALELLAGFYDACAQVEIDEYQSYEKALGAFTEAYKCLSKAKMRSPEEQERRLSDMQAKMTLVKRFIQARRAYSVDSQEAVKQCELLLEEPDLDSAIRIGDVYGLLVEHYTQQGDFQKAYQCLEEMRSKMPSVNLTYYVPQGTIEAVHRALSIPFNRHSLSEHMRHNSVEDSEEVEEVPEMDYGD

Target ORF information:

RefSeq Version	XM_031892491.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892491.1

ORF Insert Sequence:

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAA TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTACCAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA CATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGC GAGGTGAGGA GGAGAAAGCC 
TCTGAAACAG AGGTGCAGGC TCCGCAGATT CCCCAGATGG TGGCAAAGAG ACCCCTGAGA 
GACTTTATTG GGCTGGAAGA ATGCGATAAG ACCACAAGAG AAGCCATGCT CAACTTCAGC 
TTTTACCTCA CAGTGGGAGA TATGGATGAA GCATTTAAGT CTATCAAGCT TATTAAAAGT 
GAAGCAGTCT GGGAAAACAT GGCTCAAATG TGCGTTAAAA CCAAGAGGCT GGATGTCGCC 
AAGGTTTGCC TAGGAAATAT GGGCCATGCC AGGGGTGCCA AGGCCTTACG AGAGGCAGAA 
CAGGAGCCAG AGGTGGAAGC GAGAGTGGCC ATGCTGGCAA TACAGCTGGG AATGTTGGAA 
GAAGCCGAAC AACTGTACAA AAGTTGCCAG CGATACGACC TCCTGAATAA GCTTTACCAA 
GCTTGGAACC AGTGGCAGAA GGCTATTGAA ACTGCAGAAA ACCATGACAG AATCCACCTG 
CGCTCCACCT ACTACAACTA CGCCAAGCAC CTAGAGGCCA TTGGGGAACA CAATGAGTCA 
CTCCTCTTGT ATGAGAAATC TGATACTCAC CGGTTTGAGG TTCCCAGAAT GCTCCTGGAT 
GACCCCCAGG CTTTGGAAGA GTACATCAAT AAAAATAAGG ATAAGGGACT GTGGAAGTGG 
TGGGCACAAT ATTTGGAAAG CCAATCAAAC CTAGACGCTG CGCTCAAATA CTATGAGATG 
GCTCAGGACT ACCTGTCACT GGTCCGGGTG CACTGCTTCC TGGGGAATAT CCAAAGGGCA 
GCAGAAATAG CGAATGAGAC AGGTAATGGG GCTGCATCCT ACCACCTTGC GCGTCATTAT 
GAGAGCCAGG AAGAGGTGAA GCAAGCGGTG CACTTCTATA CACGTGCCCA GGCATATAAC 
AATGGCATCA GGCTCTGCAA GGAGAACAAC ATGGATGATC AGCTTATGAA CCTTGCTCTG 
CTCAGCTCCC CTGAGGACAT GATGGAGGCA GCTCGGTACT ACGAAGAGAA GGGAGAGCAA 
ATGGATAGGG CAGTCATGCT CTACCACAAG GCAGGACACT TCTCCAAGGC CTTGGAATTG 
GCTTTCACGA CAGAGCAATT TGGGGCTTTG CAGCTGATTG CTGAGGATCT GGATGAAAAG 
TCTGACCCAG CCCTCCTTGC CCGTTGCTCT GACTTTTTCA TGCAGCACAG CCAGTTTGAA 
AAGGCAGTGG AACTCCTGTT GGCAGCCAAG AAGTATTATG AGGCACTACA GCTCTGTATG 
GAACAAAACC TTGTCATTAC AGAAGAAATG GCTGAAAAAA TGACAGTGTC CAAAAATTCC 
AAGGATTTTA GCGACGAGGC CCGGCGTGAA TTGCTGCAAC GCATTGCAGA CTGCTGCATG 
AGGCAAGGGA ACTATCATCT TGCAACCAAG AAATACACTC AGGCAGGGGA TAAGTTAAAG 
GCTATGAAGT CTTTGCTTAA GTCTGGCGAC ACAGAGAAGA TTGTTTTCTT TGCTGGGGTG 
TCACGTCAGA AAGAAATTTA TATTATGGCA GCCAATTACC TGCAGTCCCT GGACTGGCGT 
AAAGATCCTG AGATTATGAA GAACATCATC AGTTTCTACA CTAAAGGCAG AGCCCTGGAA 
CTGTTAGCTG GGTTTTATGA TGCCTGTGCT CAGGTGGAGA TTGATGAATA TCAGAGCTAC 
GAAAAGGCGT TAGGAGCATT TACTGAGGCC TACAAGTGCT TGTCCAAAGC CAAAATGCGA 
AGCCCAGAGG AGCAGGAGAG GAGGCTGTCT GACATGCAAG CCAAGATGAC TCTGGTGAAG 
AGGTTTATCC AGGCTCGCAG GGCATACTCA GTGGACAGTC AAGAAGCAGT AAAGCAATGT 
GAGCTGTTGC TAGAGGAGCC GGATCTAGAC AGCGCAATAC GCATCGGGGA TGTGTATGGG 
CTACTGGTGG AGCACTATAC TCAGCAGGGG GATTTTCAGA AGGCTTACCA GTGCCTTGAG 
GAAATGCGCT CCAAGATGCC ATCTGTCAAC CTGACTTACT ACGTGCCCCA AGGTACTATA 
GAAGCTGTTC ACCGTGCCCT CTCTATTCCA TTTAACCGCC ATTCGCTGTC GGAGCACATG 
AGGCACAATA GCGTGGAGGA TAGCGAGGAG GTGGAGGAAG TACCGGAGAT GGACTATGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ift140 ( XM_031892492.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748352.1 Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X3, mRNA.

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CloneID	OXa65845
Clone ID Related Accession (Same CDS sequence)	XM_031892490.1 , XM_031892491.1 , XM_031892492.1
Accession Version	XM_031892492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAA TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTACCAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA CATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGC GAGGTGAGGA GGAGAAAGCC 
TCTGAAACAG AGGTGCAGGC TCCGCAGATT CCCCAGATGG TGGCAAAGAG ACCCCTGAGA 
GACTTTATTG GGCTGGAAGA ATGCGATAAG ACCACAAGAG AAGCCATGCT CAACTTCAGC 
TTTTACCTCA CAGTGGGAGA TATGGATGAA GCATTTAAGT CTATCAAGCT TATTAAAAGT 
GAAGCAGTCT GGGAAAACAT GGCTCAAATG TGCGTTAAAA CCAAGAGGCT GGATGTCGCC 
AAGGTTTGCC TAGGAAATAT GGGCCATGCC AGGGGTGCCA AGGCCTTACG AGAGGCAGAA 
CAGGAGCCAG AGGTGGAAGC GAGAGTGGCC ATGCTGGCAA TACAGCTGGG AATGTTGGAA 
GAAGCCGAAC AACTGTACAA AAGTTGCCAG CGATACGACC TCCTGAATAA GCTTTACCAA 
GCTTGGAACC AGTGGCAGAA GGCTATTGAA ACTGCAGAAA ACCATGACAG AATCCACCTG 
CGCTCCACCT ACTACAACTA CGCCAAGCAC CTAGAGGCCA TTGGGGAACA CAATGAGTCA 
CTCCTCTTGT ATGAGAAATC TGATACTCAC CGGTTTGAGG TTCCCAGAAT GCTCCTGGAT 
GACCCCCAGG CTTTGGAAGA GTACATCAAT AAAAATAAGG ATAAGGGACT GTGGAAGTGG 
TGGGCACAAT ATTTGGAAAG CCAATCAAAC CTAGACGCTG CGCTCAAATA CTATGAGATG 
GCTCAGGACT ACCTGTCACT GGTCCGGGTG CACTGCTTCC TGGGGAATAT CCAAAGGGCA 
GCAGAAATAG CGAATGAGAC AGGTAATGGG GCTGCATCCT ACCACCTTGC GCGTCATTAT 
GAGAGCCAGG AAGAGGTGAA GCAAGCGGTG CACTTCTATA CACGTGCCCA GGCATATAAC 
AATGGCATCA GGCTCTGCAA GGAGAACAAC ATGGATGATC AGCTTATGAA CCTTGCTCTG 
CTCAGCTCCC CTGAGGACAT GATGGAGGCA GCTCGGTACT ACGAAGAGAA GGGAGAGCAA 
ATGGATAGGG CAGTCATGCT CTACCACAAG GCAGGACACT TCTCCAAGGC CTTGGAATTG 
GCTTTCACGA CAGAGCAATT TGGGGCTTTG CAGCTGATTG CTGAGGATCT GGATGAAAAG 
TCTGACCCAG CCCTCCTTGC CCGTTGCTCT GACTTTTTCA TGCAGCACAG CCAGTTTGAA 
AAGGCAGTGG AACTCCTGTT GGCAGCCAAG AAGTATTATG AGGCACTACA GCTCTGTATG 
GAACAAAACC TTGTCATTAC AGAAGAAATG GCTGAAAAAA TGACAGTGTC CAAAAATTCC 
AAGGATTTTA GCGACGAGGC CCGGCGTGAA TTGCTGCAAC GCATTGCAGA CTGCTGCATG 
AGGCAAGGGA ACTATCATCT TGCAACCAAG AAATACACTC AGGCAGGGGA TAAGTTAAAG 
GCTATGAAGT CTTTGCTTAA GTCTGGCGAC ACAGAGAAGA TTGTTTTCTT TGCTGGGGTG 
TCACGTCAGA AAGAAATTTA TATTATGGCA GCCAATTACC TGCAGTCCCT GGACTGGCGT 
AAAGATCCTG AGATTATGAA GAACATCATC AGTTTCTACA CTAAAGGCAG AGCCCTGGAA 
CTGTTAGCTG GGTTTTATGA TGCCTGTGCT CAGGTGGAGA TTGATGAATA TCAGAGCTAC 
GAAAAGGCGT TAGGAGCATT TACTGAGGCC TACAAGTGCT TGTCCAAAGC CAAAATGCGA 
AGCCCAGAGG AGCAGGAGAG GAGGCTGTCT GACATGCAAG CCAAGATGAC TCTGGTGAAG 
AGGTTTATCC AGGCTCGCAG GGCATACTCA GTGGACAGTC AAGAAGCAGT AAAGCAATGT 
GAGCTGTTGC TAGAGGAGCC GGATCTAGAC AGCGCAATAC GCATCGGGGA TGTGTATGGG 
CTACTGGTGG AGCACTATAC TCAGCAGGGG GATTTTCAGA AGGCTTACCA GTGCCTTGAG 
GAAATGCGCT CCAAGATGCC ATCTGTCAAC CTGACTTACT ACGTGCCCCA AGGTACTATA 
GAAGCTGTTC ACCGTGCCCT CTCTATTCCA TTTAACCGCC ATTCGCTGTC GGAGCACATG 
AGGCACAATA GCGTGGAGGA TAGCGAGGAG GTGGAGGAAG TACCGGAGAT GGACTATGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748352.1
CDS	272..4657
Translation	MAVYFDHRIQAPFPSASPSHISWHKHHALLAVGYISSGDGGSVDIYNELGDHVQDAQIKRTSHPTVLSWHPTKRTLVTGWTTGELVVFNEQDKEEYTLQTTHTAEITVLEWSSGTSRLVSADMSGTVAVWRLDQRGRIQVSPLLTNMYHKPLTHCLFKPLAPDEDLAQLAKAAVSGDEKALDMFNWNKSGKWTPMKSVSQQEFHFFLSAKDGSVYFVNEQLKNSQIVTLDSPVKRLFFMEKKSILVIVTENLLLSQFFISQEGNAEEISKVKLSGKTGQAADIHWVDNSLLITATGENVIRLWDLEREDNYVLSLDDQLGYDFAETLNCISYCSAKAILAAGTNKGRVAMWSRVTSHGQKAIRTDGKGTWKLQTPTELEENIMQIQWGSSKNFLAINNFSSVVVLSEQVMSAHFSQQVAAIQVAPTQLNLTYFSTETTHNLQTDMYIKGVFVTKDTIAVWNGKMVTVYELSGPTLRNTGSFSCESPVLVMHEDNIYTVETNRVQIWTLQGTVKQLLPFSEAEGNPCGLDICGHFLAVATDTAHFKIFDLSRREAKAHINCKNLSQMVPGVGSIVSVKCNVNGSKVSILANRSDRNLDSRMYFYDAELDTVTFYDFWSGQAEQGDSISNNDPDEHDAENRVLLDRIPINHFWDQSEPRLVVCEAMLAVPKSANTSPKHQSDQQTEIHQGKEDILVISLFSTQEHGLLIQESFPRPAALQSLLGIEVPHYYFTKKRGEEEKASETEVQAPQIPQMVAKRPLRDFIGLEECDKTTREAMLNFSFYLTVGDMDEAFKSIKLIKSEAVWENMAQMCVKTKRLDVAKVCLGNMGHARGAKALREAEQEPEVEARVAMLAIQLGMLEEAEQLYKSCQRYDLLNKLYQAWNQWQKAIETAENHDRIHLRSTYYNYAKHLEAIGEHNESLLLYEKSDTHRFEVPRMLLDDPQALEEYINKNKDKGLWKWWAQYLESQSNLDAALKYYEMAQDYLSLVRVHCFLGNIQRAAEIANETGNGAASYHLARHYESQEEVKQAVHFYTRAQAYNNGIRLCKENNMDDQLMNLALLSSPEDMMEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTEQFGALQLIAEDLDEKSDPALLARCSDFFMQHSQFEKAVELLLAAKKYYEALQLCMEQNLVITEEMAEKMTVSKNSKDFSDEARRELLQRIADCCMRQGNYHLATKKYTQAGDKLKAMKSLLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKDPEIMKNIISFYTKGRALELLAGFYDACAQVEIDEYQSYEKALGAFTEAYKCLSKAKMRSPEEQERRLSDMQAKMTLVKRFIQARRAYSVDSQEAVKQCELLLEEPDLDSAIRIGDVYGLLVEHYTQQGDFQKAYQCLEEMRSKMPSVNLTYYVPQGTIEAVHRALSIPFNRHSLSEHMRHNSVEDSEEVEEVPEMDYGD

Target ORF information:

RefSeq Version	XM_031892492.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892492.1

ORF Insert Sequence:

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAA TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTACCAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA CATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGC GAGGTGAGGA GGAGAAAGCC 
TCTGAAACAG AGGTGCAGGC TCCGCAGATT CCCCAGATGG TGGCAAAGAG ACCCCTGAGA 
GACTTTATTG GGCTGGAAGA ATGCGATAAG ACCACAAGAG AAGCCATGCT CAACTTCAGC 
TTTTACCTCA CAGTGGGAGA TATGGATGAA GCATTTAAGT CTATCAAGCT TATTAAAAGT 
GAAGCAGTCT GGGAAAACAT GGCTCAAATG TGCGTTAAAA CCAAGAGGCT GGATGTCGCC 
AAGGTTTGCC TAGGAAATAT GGGCCATGCC AGGGGTGCCA AGGCCTTACG AGAGGCAGAA 
CAGGAGCCAG AGGTGGAAGC GAGAGTGGCC ATGCTGGCAA TACAGCTGGG AATGTTGGAA 
GAAGCCGAAC AACTGTACAA AAGTTGCCAG CGATACGACC TCCTGAATAA GCTTTACCAA 
GCTTGGAACC AGTGGCAGAA GGCTATTGAA ACTGCAGAAA ACCATGACAG AATCCACCTG 
CGCTCCACCT ACTACAACTA CGCCAAGCAC CTAGAGGCCA TTGGGGAACA CAATGAGTCA 
CTCCTCTTGT ATGAGAAATC TGATACTCAC CGGTTTGAGG TTCCCAGAAT GCTCCTGGAT 
GACCCCCAGG CTTTGGAAGA GTACATCAAT AAAAATAAGG ATAAGGGACT GTGGAAGTGG 
TGGGCACAAT ATTTGGAAAG CCAATCAAAC CTAGACGCTG CGCTCAAATA CTATGAGATG 
GCTCAGGACT ACCTGTCACT GGTCCGGGTG CACTGCTTCC TGGGGAATAT CCAAAGGGCA 
GCAGAAATAG CGAATGAGAC AGGTAATGGG GCTGCATCCT ACCACCTTGC GCGTCATTAT 
GAGAGCCAGG AAGAGGTGAA GCAAGCGGTG CACTTCTATA CACGTGCCCA GGCATATAAC 
AATGGCATCA GGCTCTGCAA GGAGAACAAC ATGGATGATC AGCTTATGAA CCTTGCTCTG 
CTCAGCTCCC CTGAGGACAT GATGGAGGCA GCTCGGTACT ACGAAGAGAA GGGAGAGCAA 
ATGGATAGGG CAGTCATGCT CTACCACAAG GCAGGACACT TCTCCAAGGC CTTGGAATTG 
GCTTTCACGA CAGAGCAATT TGGGGCTTTG CAGCTGATTG CTGAGGATCT GGATGAAAAG 
TCTGACCCAG CCCTCCTTGC CCGTTGCTCT GACTTTTTCA TGCAGCACAG CCAGTTTGAA 
AAGGCAGTGG AACTCCTGTT GGCAGCCAAG AAGTATTATG AGGCACTACA GCTCTGTATG 
GAACAAAACC TTGTCATTAC AGAAGAAATG GCTGAAAAAA TGACAGTGTC CAAAAATTCC 
AAGGATTTTA GCGACGAGGC CCGGCGTGAA TTGCTGCAAC GCATTGCAGA CTGCTGCATG 
AGGCAAGGGA ACTATCATCT TGCAACCAAG AAATACACTC AGGCAGGGGA TAAGTTAAAG 
GCTATGAAGT CTTTGCTTAA GTCTGGCGAC ACAGAGAAGA TTGTTTTCTT TGCTGGGGTG 
TCACGTCAGA AAGAAATTTA TATTATGGCA GCCAATTACC TGCAGTCCCT GGACTGGCGT 
AAAGATCCTG AGATTATGAA GAACATCATC AGTTTCTACA CTAAAGGCAG AGCCCTGGAA 
CTGTTAGCTG GGTTTTATGA TGCCTGTGCT CAGGTGGAGA TTGATGAATA TCAGAGCTAC 
GAAAAGGCGT TAGGAGCATT TACTGAGGCC TACAAGTGCT TGTCCAAAGC CAAAATGCGA 
AGCCCAGAGG AGCAGGAGAG GAGGCTGTCT GACATGCAAG CCAAGATGAC TCTGGTGAAG 
AGGTTTATCC AGGCTCGCAG GGCATACTCA GTGGACAGTC AAGAAGCAGT AAAGCAATGT 
GAGCTGTTGC TAGAGGAGCC GGATCTAGAC AGCGCAATAC GCATCGGGGA TGTGTATGGG 
CTACTGGTGG AGCACTATAC TCAGCAGGGG GATTTTCAGA AGGCTTACCA GTGCCTTGAG 
GAAATGCGCT CCAAGATGCC ATCTGTCAAC CTGACTTACT ACGTGCCCCA AGGTACTATA 
GAAGCTGTTC ACCGTGCCCT CTCTATTCCA TTTAACCGCC ATTCGCTGTC GGAGCACATG 
AGGCACAATA GCGTGGAGGA TAGCGAGGAG GTGGAGGAAG TACCGGAGAT GGACTATGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ift140 ( NM_001123025.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001116497.1 Xenopus tropicalis intraflagellar transport 140 (ift140), mRNA.

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CloneID	OXa21872
Clone ID Related Accession (Same CDS sequence)	NM_001123025.1
Accession Version	NM_001123025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intraflagellar transport protein 140 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC155381.1. ##Evidence-Data-START## Transcript exon combination :: BC155381.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAG TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTACCAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGCTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGAAGGGAAG CCAAAGCCCA CATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG TGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TAAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGC GAGGTGAGGA GGAGAAAGCC 
TCTGAAACAG AGGTGCAGGC TCCGCAGATT CCCCAGATGG TGGCAAAGAG ACCCCTGAGA 
GACTTTATTG GGCTGGAAGA ATGCGATAAG ACCACAAGAG AAGCCATGCT CAACTTCAGC 
TTTTACCTCA CAGTGGGAGA TATGGATGAA GCATTTAAGT CTATCAAGCT TATTAAAAGT 
GAAGCAGTCT GGGAAAACAT GGCTCGAATG TGCGTAAAGA CCAAGAGGCT GGATGTCGCC 
AAGGTTTGCC TAGGAAATAT GGGCCATGCC AGGGGTGCCA AGGCCTTACG AGAGGCAGAA 
CAGGAGCCAG AGGTGGAAGC GAGAGTGGCC ATGCTGGCAA TACAGCTGGG AATGTTGGAA 
GAAGCCGAGC AACTGTACAA AAGTTGCCTG CGATACGACC TCCTGAATAA GCTTTACCAA 
GCTTGGAACC AGTGGCAGAA GGCTATTGAA ACTGCAGAAA ACCATGACAG AATCCACCTG 
CGCTCCACCT ACTACAACTA CGCCAAGCAC CTAGAGGCCA TTGGGGAACA CAATGAGTCA 
CTCCTCTTGT ATGAGAAATC TGATACTCAC CGGTTTGAGG TTCCCAGAAT GCTCCTGGAT 
GACCCCCAGG CTTTGGAAGA GTACATCAAT AAAAATAAGG ATAAGGGACT GTGGAAATGG 
TGGGCACAAT ATTTGGAAAG CCAATCAAAC CTAGACGCTG CGCTCAAATA CTATGAGATG 
GCTCAGGACT ACCTGTCACT GGTCCGGGTG CACTGCTTCC TGGGGAATAT CCAAAGGGCA 
GCAGAAATAG CGAATGAGAC AGGTAATGGG GCTGCATCCT ACCACCTTGC GCGTCATTAT 
GAGAGCCAGG AAGAGGTGAA GCAAGCGGTG CACTTCTATA CACGTGCCCA GGCATATAAC 
AATGGCATCA GGCTCTGCAA GGAGAACAAC ATGGATGATC AGCTTATGAA CCTTGCTCTG 
CTCAGCTCCC CTGAGGACAT GATGGAGGCA GCTCGGTACT ACGAAGAGAA GGGAGAGCAA 
ATGGATAGGG CAGTCATGCT CTACCACAAG GCAGGACACT TCTCCAAGGC CTTGGAATTG 
GCTTTCACGA CAGAGCAATT TGGGGCTTTG CAGCTGATTG CTGAGGATCT GGATGAAAAG 
TCTGACCCAG CCCTCCTTGC CCGTTGCTCT GACTTTTTCA TGCAGCACAG CCAGTTTGAA 
AAGGCAGTGG AACTCCTGTT GGCAGCCAAG AAGTATTATG AGGCACTACA GCTCTGTATG 
GAACAAAACC TTGTCATTAC AGAAGAAATG GCTGAAAAAA TGACAGTGTC CAAAAATTCC 
AAGGATTTTA GCGACGAGGC CCGGCGTGAA TTGCTGCAAC GCATTGCAGA CTGCTGCATG 
AGGCAAGGGA ACTATCATCT TGCAACCAAG AAATACACTC AGGCAGGGGA TAAGTTAAAG 
GCTATGAAGT CTTTGCTTAA GTCTGGCGAC ACAGAGAAGA TTGTTTTCTT TGCTGGGGTG 
TCACGTCAGA AAGAAATTTA TATTATGGCA GCCAATTACC TGCAGTCCCT GGACTGGCGT 
AAAGATCCTG AGATTATGAA GAACATCATC AGTTTCTACA CTAAAGGCAG AGCCCTGGAA 
CTGTTAGCTG GGTTTTATGA TGCCTGTGCT CAGGTGGAGA TTGATGAATA TCAGAGCTAC 
GAAAAGGCGT TAGGAGCATT TACTGAGGCC TACAAGTGCT TGTCCAAAGC CAAAATGCGA 
AGCCCAGAGG AGCAGGAGAG GAGGCTGTCT GACATGCAAG CCAAGATGAC TCTGGTGAAG 
AGGTTTATCC AGGCTCGCAG GGCATACTCA GTGGACAGTC AAGAAGCAGT AAAGCAATGT 
GAGCTGTTGC TAGAGGAGCC GGATCTAGAC AGCGCAATAC GCATCGGGGA TGTGTATGGG 
CTACTGGTGG AGCACTATAC TCAGCAGGGG GATTTTCAGA AGGCTTACCA GTGCCTTGAG 
GAAATGCGCT CCAAGATGCC ATCTGTCAAC CTGACTTACT ACGTGCCCCA AGGTACTATA 
GAAGCTGTTC ACCGTGCCCT CTCTATTCCA TTTAACCGCC ATTCGCTGTC GGAGCACATG 
AGGCACAATA GCGTGGAGGA TAGCGAGGAG GTGGAGGAAG TACCGGAGAT GGACTATGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116497.1
CDS	172..4557
Translation	MAVYFDHRIQAPFPSASPSHISWHKHHALLAVGYISSGDGGSVDIYSELGDHVQDAQIKRTSHPTVLSWHPTKRTLVTGWTTGELVVFNEQDKEEYTLQTTHTAEITVLEWSSGTSRLVSADMSGTVAVWRLDQRGRIQVSPLLTNMYHKPLTHCLFKPLAPDEDLAQLAKAAVSGDEKALDMFNWNKSGKWTPMKSVSQQEFHFFLSAKDGSVYFVNEQLKNSQIVTLDSPVKRLFFMEKKSILVIVTENLLLSQFFISQEGNAEEISKVKLSGKTGQAADIHWVDNSLLITATGENVIRLWDLEREDNYVLSLDDQLGYDFAETLNCISYCSAKAILAAGTNKGRVAMWSRVTSHGQKAIRTDGKGTWKLQTPTELEENIMQIQWGSSKNFLAINNFSSVVVLSEQVMSAHFSQQVAAIQVAPTQLNLTYFSTETTHNLQTDMYIKGVFVTKDTIAVWNGKMVTVYELSGPTLRNTGSFSCESPVLVMHEDNIYTVETNRVQIWTLQGTVKQLLPFSEAEGNPCGLDICGHFLAVATDTAHFKIFDLSRREAKAHINCKNLSQMVPGVGSIVSVKCNVNGSKVSILANRSDRNLDSRMYFYDAELDTVTFYDFWSGQAEQGDSISNNDPDEHDAENRVLLDRIPINHFWDQSEPRLVVCEAMLAVPKSANTSPKHKSDQQTEIHQGKEDILVISLFSTQEHGLLIQESFPRPAALQSLLGIEVPHYYFTKKRGEEEKASETEVQAPQIPQMVAKRPLRDFIGLEECDKTTREAMLNFSFYLTVGDMDEAFKSIKLIKSEAVWENMARMCVKTKRLDVAKVCLGNMGHARGAKALREAEQEPEVEARVAMLAIQLGMLEEAEQLYKSCLRYDLLNKLYQAWNQWQKAIETAENHDRIHLRSTYYNYAKHLEAIGEHNESLLLYEKSDTHRFEVPRMLLDDPQALEEYINKNKDKGLWKWWAQYLESQSNLDAALKYYEMAQDYLSLVRVHCFLGNIQRAAEIANETGNGAASYHLARHYESQEEVKQAVHFYTRAQAYNNGIRLCKENNMDDQLMNLALLSSPEDMMEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTEQFGALQLIAEDLDEKSDPALLARCSDFFMQHSQFEKAVELLLAAKKYYEALQLCMEQNLVITEEMAEKMTVSKNSKDFSDEARRELLQRIADCCMRQGNYHLATKKYTQAGDKLKAMKSLLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKDPEIMKNIISFYTKGRALELLAGFYDACAQVEIDEYQSYEKALGAFTEAYKCLSKAKMRSPEEQERRLSDMQAKMTLVKRFIQARRAYSVDSQEAVKQCELLLEEPDLDSAIRIGDVYGLLVEHYTQQGDFQKAYQCLEEMRSKMPSVNLTYYVPQGTIEAVHRALSIPFNRHSLSEHMRHNSVEDSEEVEEVPEMDYGD

Target ORF information:

RefSeq Version	NM_001123025.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intraflagellar transport 140 (ift140), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123025.1

ORF Insert Sequence:

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAG TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTACCAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGCTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGAAGGGAAG CCAAAGCCCA CATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG TGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TAAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGC GAGGTGAGGA GGAGAAAGCC 
TCTGAAACAG AGGTGCAGGC TCCGCAGATT CCCCAGATGG TGGCAAAGAG ACCCCTGAGA 
GACTTTATTG GGCTGGAAGA ATGCGATAAG ACCACAAGAG AAGCCATGCT CAACTTCAGC 
TTTTACCTCA CAGTGGGAGA TATGGATGAA GCATTTAAGT CTATCAAGCT TATTAAAAGT 
GAAGCAGTCT GGGAAAACAT GGCTCGAATG TGCGTAAAGA CCAAGAGGCT GGATGTCGCC 
AAGGTTTGCC TAGGAAATAT GGGCCATGCC AGGGGTGCCA AGGCCTTACG AGAGGCAGAA 
CAGGAGCCAG AGGTGGAAGC GAGAGTGGCC ATGCTGGCAA TACAGCTGGG AATGTTGGAA 
GAAGCCGAGC AACTGTACAA AAGTTGCCTG CGATACGACC TCCTGAATAA GCTTTACCAA 
GCTTGGAACC AGTGGCAGAA GGCTATTGAA ACTGCAGAAA ACCATGACAG AATCCACCTG 
CGCTCCACCT ACTACAACTA CGCCAAGCAC CTAGAGGCCA TTGGGGAACA CAATGAGTCA 
CTCCTCTTGT ATGAGAAATC TGATACTCAC CGGTTTGAGG TTCCCAGAAT GCTCCTGGAT 
GACCCCCAGG CTTTGGAAGA GTACATCAAT AAAAATAAGG ATAAGGGACT GTGGAAATGG 
TGGGCACAAT ATTTGGAAAG CCAATCAAAC CTAGACGCTG CGCTCAAATA CTATGAGATG 
GCTCAGGACT ACCTGTCACT GGTCCGGGTG CACTGCTTCC TGGGGAATAT CCAAAGGGCA 
GCAGAAATAG CGAATGAGAC AGGTAATGGG GCTGCATCCT ACCACCTTGC GCGTCATTAT 
GAGAGCCAGG AAGAGGTGAA GCAAGCGGTG CACTTCTATA CACGTGCCCA GGCATATAAC 
AATGGCATCA GGCTCTGCAA GGAGAACAAC ATGGATGATC AGCTTATGAA CCTTGCTCTG 
CTCAGCTCCC CTGAGGACAT GATGGAGGCA GCTCGGTACT ACGAAGAGAA GGGAGAGCAA 
ATGGATAGGG CAGTCATGCT CTACCACAAG GCAGGACACT TCTCCAAGGC CTTGGAATTG 
GCTTTCACGA CAGAGCAATT TGGGGCTTTG CAGCTGATTG CTGAGGATCT GGATGAAAAG 
TCTGACCCAG CCCTCCTTGC CCGTTGCTCT GACTTTTTCA TGCAGCACAG CCAGTTTGAA 
AAGGCAGTGG AACTCCTGTT GGCAGCCAAG AAGTATTATG AGGCACTACA GCTCTGTATG 
GAACAAAACC TTGTCATTAC AGAAGAAATG GCTGAAAAAA TGACAGTGTC CAAAAATTCC 
AAGGATTTTA GCGACGAGGC CCGGCGTGAA TTGCTGCAAC GCATTGCAGA CTGCTGCATG 
AGGCAAGGGA ACTATCATCT TGCAACCAAG AAATACACTC AGGCAGGGGA TAAGTTAAAG 
GCTATGAAGT CTTTGCTTAA GTCTGGCGAC ACAGAGAAGA TTGTTTTCTT TGCTGGGGTG 
TCACGTCAGA AAGAAATTTA TATTATGGCA GCCAATTACC TGCAGTCCCT GGACTGGCGT 
AAAGATCCTG AGATTATGAA GAACATCATC AGTTTCTACA CTAAAGGCAG AGCCCTGGAA 
CTGTTAGCTG GGTTTTATGA TGCCTGTGCT CAGGTGGAGA TTGATGAATA TCAGAGCTAC 
GAAAAGGCGT TAGGAGCATT TACTGAGGCC TACAAGTGCT TGTCCAAAGC CAAAATGCGA 
AGCCCAGAGG AGCAGGAGAG GAGGCTGTCT GACATGCAAG CCAAGATGAC TCTGGTGAAG 
AGGTTTATCC AGGCTCGCAG GGCATACTCA GTGGACAGTC AAGAAGCAGT AAAGCAATGT 
GAGCTGTTGC TAGAGGAGCC GGATCTAGAC AGCGCAATAC GCATCGGGGA TGTGTATGGG 
CTACTGGTGG AGCACTATAC TCAGCAGGGG GATTTTCAGA AGGCTTACCA GTGCCTTGAG 
GAAATGCGCT CCAAGATGCC ATCTGTCAAC CTGACTTACT ACGTGCCCCA AGGTACTATA 
GAAGCTGTTC ACCGTGCCCT CTCTATTCCA TTTAACCGCC ATTCGCTGTC GGAGCACATG 
AGGCACAATA GCGTGGAGGA TAGCGAGGAG GTGGAGGAAG TACCGGAGAT GGACTATGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ift140 ( XM_012970030.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825484.1 Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa31338
Clone ID Related Accession (Same CDS sequence)	XM_012970030.2 , XM_012970031.2 , XM_012970029.2 , XM_018097033.1
Accession Version	XM_012970030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAG TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTAACAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA TATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGT TGTTTTTCCG GAGGGGTTGT 
TTCTATCAGC CAGTTCGAGG TGAGGAGGAG AAAGCCTCTG AAACAGAGGT GCAGGCTCCG 
CAGATTCCCC AGATGGTGGC AAAGAGACCC CTGAGAGACT TTATTGGGCT GGAAGAATGC 
GATAAGACCA CAAGAGAAGC CATGCTCAAC TTCAGCTTTT ACCTCACAGT GGGAGATATG 
GATGAAGCAT TTAAGTCTAT CAAGCTTATT AAAAGTGAAG CAGTCTGGGA AAACATGGCT 
CGAATGTGCG TAAAGACCAA GAGGCTGGAT GTCGCCAAGG TTTGCCTAGG AAATATGGGC 
CATGCCAGGG GTGCCAAGGC CTTACGAGAG GCAGAACAGG AGCCAGAGGT GGAAGCGAGA 
GTGGCCATGC TGGCAATACA GCTGGGAATG TTGGAAGAAG CCGAGCAACT GTACAAAAGT 
TGCCTGCGAT ACGACCTCCT GAATAAGCTT TACCAAGCTT GGAACCAGTG GCAGAAGGCT 
ATTGAAACTG CAGAAAACCA TGACAGAATC CACCTGCGCT CCACCTACTA CAACTACGCC 
AAGCACCTAG AGGCCATTGG GGAACACAAT GAGTCACTCC TCTTGTATGA GAAATCTGAT 
ACTCACCGGT TTGAGGTTCC CAGAATGCTC CTGGATGACC CCCAGGCTTT GGAAGAGTAC 
ATCAATAAAA ATAAGGATAA GGGACTGTGG AAGTGGTGGG CACAATATTT GGAAAGCCAA 
TCAAACCTAG ACGCTGCGCT CAAATACTAT GAGATGGCTC AGGACTACCT GTCACTGGTC 
CGGGTGCACT GCTTCCTGGG GAATATCCAA AGGGCAGCAG AAATAGCGAA TGAGACAGGT 
AATGGGGCTG CATCCTACCA CCTTGCGCGT CATTATGAGA GCCAGGAAGA GGTGAAGCAA 
GCGGTGCACT TCTATACACG TGCCCAGGCA TATAACAATG GCATCAGGCT CTGCAAGGAG 
AACAACATGG ATGATCAGCT TATGAACCTT GCTCTGCTCA GCTCCCCTGA GGACATGATG 
GAGGCAGCTC GGTACTACGA AGAGAAGGGA GAGCAAATGG ATAGGGCAGT CATGCTCTAC 
CACAAGGCAG GACACTTCTC CAAGGCCTTG GAATTGGCTT TCACGACAGA GCAATTTGGG 
GCTTTGCAGC TGATTGCTGA GGATCTGGAT GAAAAGTCTG ACCCAGCCCT CCTTGCCCGT 
TGCTCTGACT TTTTCATGCA GCACAGCCAG TTTGAAAAGG CAGTGGAACT CCTGTTGGCA 
GCCAAGAAGT ATTATGAGGC ACTACAGCTC TGTATGGAAC AAAACCTTGT CATTACAGAA 
GAAATGGCTG AAAAAATGAC AGTGTCCAAA AATTCCAAGG ATTTTAGCGA CGAGGCCCGG 
CGTGAATTGC TGCAACGCAT TGCAGACTGC TGCATGAGGC AAGGGAACTA TCATCTTGCA 
ACCAAGAAAT ACACTCAGGC AGGGGATAAG TTAAAGGCTA TGAAGTCTTT GCTTAAGTCT 
GGCGACACAG AGAAGATTGT TTTCTTTGCT GGGGTGTCAC GTCAGAAAGA AATTTATATT 
ATGGCAGCCA ATTACCTGCA GTCCCTGGAC TGGCGTAAAG ATCCTGAGAT TATGAAGAAC 
ATCATCAGTT TCTACACTAA AGGCAGAGCC CTGGAACTGT TAGCTGGGTT TTATGATGCC 
TGTGCTCAGG TGGAGATTGA TGAATATCAG AGCTACGAAA AGGCGTTAGG AGCATTTACT 
GAGGCCTACA AGTGCTTGTC CAAAGCCAAA ATGCGAAGCC CAGAGGAGCA GGAGAGGAGG 
CTGTCTGACA TGCAAGCCAA GATGACTCTG GTGAAGAGGT TTATCCAGGC TCGCAGGGCA 
TACTCAGTGG ACAGTCAAGA AGCAGTAAAG CAATGTGAGC TGTTGCTAGA GGAGCCGGAT 
CTAGACAGCG CAATACGCAT CGGGGATGTG TATGGGCTAC TGGTGGAGCA CTATACTCAG 
CAGGGGGATT TTCAGAAGGC TTACCAGTGC CTTGAGGAAA TGCGCTCCAA GATGCCATCT 
GTCAACCTGA CTTACTACGT GCCCCAAGGT ACTATAGAAG CTGTTCACCG TGCCCTCTCT 
ATTCCATTTA ACCGCCATTC GCTGTCGGAG CACATGAGGC ACAATAGCGT GGAGGATAGC 
GAGGAGGTGG AGGAAGTACC GGAGATGGAC TATGGAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825484.1
CDS	384..4805
Translation	MAVYFDHRIQAPFPSASPSHISWHKHHALLAVGYISSGDGGSVDIYSELGDHVQDAQIKRTSHPTVLSWHPTKRTLVTGWTTGELVVFNEQDKEEYTLQTTHTAEITVLEWSSGNSRLVSADMSGTVAVWRLDQRGRIQVSPLLTNMYHKPLTHCLFKPLAPDEDLAQLAKAAVSGDEKALDMFNWNKSGKWTPMKSVSQQEFHFFLSAKDGSVYFVNEQLKNSQIVTLDSPVKRLFFMEKKSILVIVTENLLLSQFFISQEGNAEEISKVKLSGKTGQAADIHWVDNSLLITATGENVIRLWDLEREDNYVLSLDDQLGYDFAETLNCISYCSAKAILAAGTNKGRVAMWSRVTSHGQKAIRTDGKGTWKLQTPTELEENIMQIQWGSSKNFLAINNFSSVVVLSEQVMSAHFSQQVAAIQVAPTQLNLTYFSTETTHNLQTDMYIKGVFVTKDTIAVWNGKMVTVYELSGPTLRNTGSFSCESPVLVMHEDNIYTVETNRVQIWTLQGTVKQLLPFSEAEGNPCGLDICGHFLAVATDTAHFKIFDLSRREAKAHINCKNLSQMVPGVGSIVSVKCNVNGSKVSILANRSDRNLDSRMYFYDAELDTVTFYDFWSGQAEQGDSISNNDPDEHDAENRVLLDRIPINHFWDQSEPRLVVCEAMLAVPKSANTSPKHQSDQQTEIHQGKEDILVISLFSTQEHGLLIQESFPRPAALQSLLGIEVPHYYFTKKLFFRRGCFYQPVRGEEEKASETEVQAPQIPQMVAKRPLRDFIGLEECDKTTREAMLNFSFYLTVGDMDEAFKSIKLIKSEAVWENMARMCVKTKRLDVAKVCLGNMGHARGAKALREAEQEPEVEARVAMLAIQLGMLEEAEQLYKSCLRYDLLNKLYQAWNQWQKAIETAENHDRIHLRSTYYNYAKHLEAIGEHNESLLLYEKSDTHRFEVPRMLLDDPQALEEYINKNKDKGLWKWWAQYLESQSNLDAALKYYEMAQDYLSLVRVHCFLGNIQRAAEIANETGNGAASYHLARHYESQEEVKQAVHFYTRAQAYNNGIRLCKENNMDDQLMNLALLSSPEDMMEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTEQFGALQLIAEDLDEKSDPALLARCSDFFMQHSQFEKAVELLLAAKKYYEALQLCMEQNLVITEEMAEKMTVSKNSKDFSDEARRELLQRIADCCMRQGNYHLATKKYTQAGDKLKAMKSLLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKDPEIMKNIISFYTKGRALELLAGFYDACAQVEIDEYQSYEKALGAFTEAYKCLSKAKMRSPEEQERRLSDMQAKMTLVKRFIQARRAYSVDSQEAVKQCELLLEEPDLDSAIRIGDVYGLLVEHYTQQGDFQKAYQCLEEMRSKMPSVNLTYYVPQGTIEAVHRALSIPFNRHSLSEHMRHNSVEDSEEVEEVPEMDYGD

Target ORF information:

RefSeq Version	XM_012970030.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970030.2

ORF Insert Sequence:

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAG TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTAACAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA TATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGT TGTTTTTCCG GAGGGGTTGT 
TTCTATCAGC CAGTTCGAGG TGAGGAGGAG AAAGCCTCTG AAACAGAGGT GCAGGCTCCG 
CAGATTCCCC AGATGGTGGC AAAGAGACCC CTGAGAGACT TTATTGGGCT GGAAGAATGC 
GATAAGACCA CAAGAGAAGC CATGCTCAAC TTCAGCTTTT ACCTCACAGT GGGAGATATG 
GATGAAGCAT TTAAGTCTAT CAAGCTTATT AAAAGTGAAG CAGTCTGGGA AAACATGGCT 
CGAATGTGCG TAAAGACCAA GAGGCTGGAT GTCGCCAAGG TTTGCCTAGG AAATATGGGC 
CATGCCAGGG GTGCCAAGGC CTTACGAGAG GCAGAACAGG AGCCAGAGGT GGAAGCGAGA 
GTGGCCATGC TGGCAATACA GCTGGGAATG TTGGAAGAAG CCGAGCAACT GTACAAAAGT 
TGCCTGCGAT ACGACCTCCT GAATAAGCTT TACCAAGCTT GGAACCAGTG GCAGAAGGCT 
ATTGAAACTG CAGAAAACCA TGACAGAATC CACCTGCGCT CCACCTACTA CAACTACGCC 
AAGCACCTAG AGGCCATTGG GGAACACAAT GAGTCACTCC TCTTGTATGA GAAATCTGAT 
ACTCACCGGT TTGAGGTTCC CAGAATGCTC CTGGATGACC CCCAGGCTTT GGAAGAGTAC 
ATCAATAAAA ATAAGGATAA GGGACTGTGG AAGTGGTGGG CACAATATTT GGAAAGCCAA 
TCAAACCTAG ACGCTGCGCT CAAATACTAT GAGATGGCTC AGGACTACCT GTCACTGGTC 
CGGGTGCACT GCTTCCTGGG GAATATCCAA AGGGCAGCAG AAATAGCGAA TGAGACAGGT 
AATGGGGCTG CATCCTACCA CCTTGCGCGT CATTATGAGA GCCAGGAAGA GGTGAAGCAA 
GCGGTGCACT TCTATACACG TGCCCAGGCA TATAACAATG GCATCAGGCT CTGCAAGGAG 
AACAACATGG ATGATCAGCT TATGAACCTT GCTCTGCTCA GCTCCCCTGA GGACATGATG 
GAGGCAGCTC GGTACTACGA AGAGAAGGGA GAGCAAATGG ATAGGGCAGT CATGCTCTAC 
CACAAGGCAG GACACTTCTC CAAGGCCTTG GAATTGGCTT TCACGACAGA GCAATTTGGG 
GCTTTGCAGC TGATTGCTGA GGATCTGGAT GAAAAGTCTG ACCCAGCCCT CCTTGCCCGT 
TGCTCTGACT TTTTCATGCA GCACAGCCAG TTTGAAAAGG CAGTGGAACT CCTGTTGGCA 
GCCAAGAAGT ATTATGAGGC ACTACAGCTC TGTATGGAAC AAAACCTTGT CATTACAGAA 
GAAATGGCTG AAAAAATGAC AGTGTCCAAA AATTCCAAGG ATTTTAGCGA CGAGGCCCGG 
CGTGAATTGC TGCAACGCAT TGCAGACTGC TGCATGAGGC AAGGGAACTA TCATCTTGCA 
ACCAAGAAAT ACACTCAGGC AGGGGATAAG TTAAAGGCTA TGAAGTCTTT GCTTAAGTCT 
GGCGACACAG AGAAGATTGT TTTCTTTGCT GGGGTGTCAC GTCAGAAAGA AATTTATATT 
ATGGCAGCCA ATTACCTGCA GTCCCTGGAC TGGCGTAAAG ATCCTGAGAT TATGAAGAAC 
ATCATCAGTT TCTACACTAA AGGCAGAGCC CTGGAACTGT TAGCTGGGTT TTATGATGCC 
TGTGCTCAGG TGGAGATTGA TGAATATCAG AGCTACGAAA AGGCGTTAGG AGCATTTACT 
GAGGCCTACA AGTGCTTGTC CAAAGCCAAA ATGCGAAGCC CAGAGGAGCA GGAGAGGAGG 
CTGTCTGACA TGCAAGCCAA GATGACTCTG GTGAAGAGGT TTATCCAGGC TCGCAGGGCA 
TACTCAGTGG ACAGTCAAGA AGCAGTAAAG CAATGTGAGC TGTTGCTAGA GGAGCCGGAT 
CTAGACAGCG CAATACGCAT CGGGGATGTG TATGGGCTAC TGGTGGAGCA CTATACTCAG 
CAGGGGGATT TTCAGAAGGC TTACCAGTGC CTTGAGGAAA TGCGCTCCAA GATGCCATCT 
GTCAACCTGA CTTACTACGT GCCCCAAGGT ACTATAGAAG CTGTTCACCG TGCCCTCTCT 
ATTCCATTTA ACCGCCATTC GCTGTCGGAG CACATGAGGC ACAATAGCGT GGAGGATAGC 
GAGGAGGTGG AGGAAGTACC GGAGATGGAC TATGGAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ift140 ( XM_012970031.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825485.1 Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa31338
Clone ID Related Accession (Same CDS sequence)	XM_012970030.2 , XM_012970031.2 , XM_012970029.2 , XM_018097033.1
Accession Version	XM_012970031.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAG TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTAACAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA TATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGT TGTTTTTCCG GAGGGGTTGT 
TTCTATCAGC CAGTTCGAGG TGAGGAGGAG AAAGCCTCTG AAACAGAGGT GCAGGCTCCG 
CAGATTCCCC AGATGGTGGC AAAGAGACCC CTGAGAGACT TTATTGGGCT GGAAGAATGC 
GATAAGACCA CAAGAGAAGC CATGCTCAAC TTCAGCTTTT ACCTCACAGT GGGAGATATG 
GATGAAGCAT TTAAGTCTAT CAAGCTTATT AAAAGTGAAG CAGTCTGGGA AAACATGGCT 
CGAATGTGCG TAAAGACCAA GAGGCTGGAT GTCGCCAAGG TTTGCCTAGG AAATATGGGC 
CATGCCAGGG GTGCCAAGGC CTTACGAGAG GCAGAACAGG AGCCAGAGGT GGAAGCGAGA 
GTGGCCATGC TGGCAATACA GCTGGGAATG TTGGAAGAAG CCGAGCAACT GTACAAAAGT 
TGCCTGCGAT ACGACCTCCT GAATAAGCTT TACCAAGCTT GGAACCAGTG GCAGAAGGCT 
ATTGAAACTG CAGAAAACCA TGACAGAATC CACCTGCGCT CCACCTACTA CAACTACGCC 
AAGCACCTAG AGGCCATTGG GGAACACAAT GAGTCACTCC TCTTGTATGA GAAATCTGAT 
ACTCACCGGT TTGAGGTTCC CAGAATGCTC CTGGATGACC CCCAGGCTTT GGAAGAGTAC 
ATCAATAAAA ATAAGGATAA GGGACTGTGG AAGTGGTGGG CACAATATTT GGAAAGCCAA 
TCAAACCTAG ACGCTGCGCT CAAATACTAT GAGATGGCTC AGGACTACCT GTCACTGGTC 
CGGGTGCACT GCTTCCTGGG GAATATCCAA AGGGCAGCAG AAATAGCGAA TGAGACAGGT 
AATGGGGCTG CATCCTACCA CCTTGCGCGT CATTATGAGA GCCAGGAAGA GGTGAAGCAA 
GCGGTGCACT TCTATACACG TGCCCAGGCA TATAACAATG GCATCAGGCT CTGCAAGGAG 
AACAACATGG ATGATCAGCT TATGAACCTT GCTCTGCTCA GCTCCCCTGA GGACATGATG 
GAGGCAGCTC GGTACTACGA AGAGAAGGGA GAGCAAATGG ATAGGGCAGT CATGCTCTAC 
CACAAGGCAG GACACTTCTC CAAGGCCTTG GAATTGGCTT TCACGACAGA GCAATTTGGG 
GCTTTGCAGC TGATTGCTGA GGATCTGGAT GAAAAGTCTG ACCCAGCCCT CCTTGCCCGT 
TGCTCTGACT TTTTCATGCA GCACAGCCAG TTTGAAAAGG CAGTGGAACT CCTGTTGGCA 
GCCAAGAAGT ATTATGAGGC ACTACAGCTC TGTATGGAAC AAAACCTTGT CATTACAGAA 
GAAATGGCTG AAAAAATGAC AGTGTCCAAA AATTCCAAGG ATTTTAGCGA CGAGGCCCGG 
CGTGAATTGC TGCAACGCAT TGCAGACTGC TGCATGAGGC AAGGGAACTA TCATCTTGCA 
ACCAAGAAAT ACACTCAGGC AGGGGATAAG TTAAAGGCTA TGAAGTCTTT GCTTAAGTCT 
GGCGACACAG AGAAGATTGT TTTCTTTGCT GGGGTGTCAC GTCAGAAAGA AATTTATATT 
ATGGCAGCCA ATTACCTGCA GTCCCTGGAC TGGCGTAAAG ATCCTGAGAT TATGAAGAAC 
ATCATCAGTT TCTACACTAA AGGCAGAGCC CTGGAACTGT TAGCTGGGTT TTATGATGCC 
TGTGCTCAGG TGGAGATTGA TGAATATCAG AGCTACGAAA AGGCGTTAGG AGCATTTACT 
GAGGCCTACA AGTGCTTGTC CAAAGCCAAA ATGCGAAGCC CAGAGGAGCA GGAGAGGAGG 
CTGTCTGACA TGCAAGCCAA GATGACTCTG GTGAAGAGGT TTATCCAGGC TCGCAGGGCA 
TACTCAGTGG ACAGTCAAGA AGCAGTAAAG CAATGTGAGC TGTTGCTAGA GGAGCCGGAT 
CTAGACAGCG CAATACGCAT CGGGGATGTG TATGGGCTAC TGGTGGAGCA CTATACTCAG 
CAGGGGGATT TTCAGAAGGC TTACCAGTGC CTTGAGGAAA TGCGCTCCAA GATGCCATCT 
GTCAACCTGA CTTACTACGT GCCCCAAGGT ACTATAGAAG CTGTTCACCG TGCCCTCTCT 
ATTCCATTTA ACCGCCATTC GCTGTCGGAG CACATGAGGC ACAATAGCGT GGAGGATAGC 
GAGGAGGTGG AGGAAGTACC GGAGATGGAC TATGGAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825485.1
CDS	284..4705
Translation	MAVYFDHRIQAPFPSASPSHISWHKHHALLAVGYISSGDGGSVDIYSELGDHVQDAQIKRTSHPTVLSWHPTKRTLVTGWTTGELVVFNEQDKEEYTLQTTHTAEITVLEWSSGNSRLVSADMSGTVAVWRLDQRGRIQVSPLLTNMYHKPLTHCLFKPLAPDEDLAQLAKAAVSGDEKALDMFNWNKSGKWTPMKSVSQQEFHFFLSAKDGSVYFVNEQLKNSQIVTLDSPVKRLFFMEKKSILVIVTENLLLSQFFISQEGNAEEISKVKLSGKTGQAADIHWVDNSLLITATGENVIRLWDLEREDNYVLSLDDQLGYDFAETLNCISYCSAKAILAAGTNKGRVAMWSRVTSHGQKAIRTDGKGTWKLQTPTELEENIMQIQWGSSKNFLAINNFSSVVVLSEQVMSAHFSQQVAAIQVAPTQLNLTYFSTETTHNLQTDMYIKGVFVTKDTIAVWNGKMVTVYELSGPTLRNTGSFSCESPVLVMHEDNIYTVETNRVQIWTLQGTVKQLLPFSEAEGNPCGLDICGHFLAVATDTAHFKIFDLSRREAKAHINCKNLSQMVPGVGSIVSVKCNVNGSKVSILANRSDRNLDSRMYFYDAELDTVTFYDFWSGQAEQGDSISNNDPDEHDAENRVLLDRIPINHFWDQSEPRLVVCEAMLAVPKSANTSPKHQSDQQTEIHQGKEDILVISLFSTQEHGLLIQESFPRPAALQSLLGIEVPHYYFTKKLFFRRGCFYQPVRGEEEKASETEVQAPQIPQMVAKRPLRDFIGLEECDKTTREAMLNFSFYLTVGDMDEAFKSIKLIKSEAVWENMARMCVKTKRLDVAKVCLGNMGHARGAKALREAEQEPEVEARVAMLAIQLGMLEEAEQLYKSCLRYDLLNKLYQAWNQWQKAIETAENHDRIHLRSTYYNYAKHLEAIGEHNESLLLYEKSDTHRFEVPRMLLDDPQALEEYINKNKDKGLWKWWAQYLESQSNLDAALKYYEMAQDYLSLVRVHCFLGNIQRAAEIANETGNGAASYHLARHYESQEEVKQAVHFYTRAQAYNNGIRLCKENNMDDQLMNLALLSSPEDMMEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTEQFGALQLIAEDLDEKSDPALLARCSDFFMQHSQFEKAVELLLAAKKYYEALQLCMEQNLVITEEMAEKMTVSKNSKDFSDEARRELLQRIADCCMRQGNYHLATKKYTQAGDKLKAMKSLLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKDPEIMKNIISFYTKGRALELLAGFYDACAQVEIDEYQSYEKALGAFTEAYKCLSKAKMRSPEEQERRLSDMQAKMTLVKRFIQARRAYSVDSQEAVKQCELLLEEPDLDSAIRIGDVYGLLVEHYTQQGDFQKAYQCLEEMRSKMPSVNLTYYVPQGTIEAVHRALSIPFNRHSLSEHMRHNSVEDSEEVEEVPEMDYGD

Target ORF information:

RefSeq Version	XM_012970031.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970031.2

ORF Insert Sequence:

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAG TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTAACAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA TATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGT TGTTTTTCCG GAGGGGTTGT 
TTCTATCAGC CAGTTCGAGG TGAGGAGGAG AAAGCCTCTG AAACAGAGGT GCAGGCTCCG 
CAGATTCCCC AGATGGTGGC AAAGAGACCC CTGAGAGACT TTATTGGGCT GGAAGAATGC 
GATAAGACCA CAAGAGAAGC CATGCTCAAC TTCAGCTTTT ACCTCACAGT GGGAGATATG 
GATGAAGCAT TTAAGTCTAT CAAGCTTATT AAAAGTGAAG CAGTCTGGGA AAACATGGCT 
CGAATGTGCG TAAAGACCAA GAGGCTGGAT GTCGCCAAGG TTTGCCTAGG AAATATGGGC 
CATGCCAGGG GTGCCAAGGC CTTACGAGAG GCAGAACAGG AGCCAGAGGT GGAAGCGAGA 
GTGGCCATGC TGGCAATACA GCTGGGAATG TTGGAAGAAG CCGAGCAACT GTACAAAAGT 
TGCCTGCGAT ACGACCTCCT GAATAAGCTT TACCAAGCTT GGAACCAGTG GCAGAAGGCT 
ATTGAAACTG CAGAAAACCA TGACAGAATC CACCTGCGCT CCACCTACTA CAACTACGCC 
AAGCACCTAG AGGCCATTGG GGAACACAAT GAGTCACTCC TCTTGTATGA GAAATCTGAT 
ACTCACCGGT TTGAGGTTCC CAGAATGCTC CTGGATGACC CCCAGGCTTT GGAAGAGTAC 
ATCAATAAAA ATAAGGATAA GGGACTGTGG AAGTGGTGGG CACAATATTT GGAAAGCCAA 
TCAAACCTAG ACGCTGCGCT CAAATACTAT GAGATGGCTC AGGACTACCT GTCACTGGTC 
CGGGTGCACT GCTTCCTGGG GAATATCCAA AGGGCAGCAG AAATAGCGAA TGAGACAGGT 
AATGGGGCTG CATCCTACCA CCTTGCGCGT CATTATGAGA GCCAGGAAGA GGTGAAGCAA 
GCGGTGCACT TCTATACACG TGCCCAGGCA TATAACAATG GCATCAGGCT CTGCAAGGAG 
AACAACATGG ATGATCAGCT TATGAACCTT GCTCTGCTCA GCTCCCCTGA GGACATGATG 
GAGGCAGCTC GGTACTACGA AGAGAAGGGA GAGCAAATGG ATAGGGCAGT CATGCTCTAC 
CACAAGGCAG GACACTTCTC CAAGGCCTTG GAATTGGCTT TCACGACAGA GCAATTTGGG 
GCTTTGCAGC TGATTGCTGA GGATCTGGAT GAAAAGTCTG ACCCAGCCCT CCTTGCCCGT 
TGCTCTGACT TTTTCATGCA GCACAGCCAG TTTGAAAAGG CAGTGGAACT CCTGTTGGCA 
GCCAAGAAGT ATTATGAGGC ACTACAGCTC TGTATGGAAC AAAACCTTGT CATTACAGAA 
GAAATGGCTG AAAAAATGAC AGTGTCCAAA AATTCCAAGG ATTTTAGCGA CGAGGCCCGG 
CGTGAATTGC TGCAACGCAT TGCAGACTGC TGCATGAGGC AAGGGAACTA TCATCTTGCA 
ACCAAGAAAT ACACTCAGGC AGGGGATAAG TTAAAGGCTA TGAAGTCTTT GCTTAAGTCT 
GGCGACACAG AGAAGATTGT TTTCTTTGCT GGGGTGTCAC GTCAGAAAGA AATTTATATT 
ATGGCAGCCA ATTACCTGCA GTCCCTGGAC TGGCGTAAAG ATCCTGAGAT TATGAAGAAC 
ATCATCAGTT TCTACACTAA AGGCAGAGCC CTGGAACTGT TAGCTGGGTT TTATGATGCC 
TGTGCTCAGG TGGAGATTGA TGAATATCAG AGCTACGAAA AGGCGTTAGG AGCATTTACT 
GAGGCCTACA AGTGCTTGTC CAAAGCCAAA ATGCGAAGCC CAGAGGAGCA GGAGAGGAGG 
CTGTCTGACA TGCAAGCCAA GATGACTCTG GTGAAGAGGT TTATCCAGGC TCGCAGGGCA 
TACTCAGTGG ACAGTCAAGA AGCAGTAAAG CAATGTGAGC TGTTGCTAGA GGAGCCGGAT 
CTAGACAGCG CAATACGCAT CGGGGATGTG TATGGGCTAC TGGTGGAGCA CTATACTCAG 
CAGGGGGATT TTCAGAAGGC TTACCAGTGC CTTGAGGAAA TGCGCTCCAA GATGCCATCT 
GTCAACCTGA CTTACTACGT GCCCCAAGGT ACTATAGAAG CTGTTCACCG TGCCCTCTCT 
ATTCCATTTA ACCGCCATTC GCTGTCGGAG CACATGAGGC ACAATAGCGT GGAGGATAGC 
GAGGAGGTGG AGGAAGTACC GGAGATGGAC TATGGAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ift140 ( XM_012970029.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825483.1 Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X1, mRNA.

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CloneID	OXa31338
Clone ID Related Accession (Same CDS sequence)	XM_012970030.2 , XM_012970031.2 , XM_012970029.2 , XM_018097033.1
Accession Version	XM_012970029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAG TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTAACAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA TATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGT TGTTTTTCCG GAGGGGTTGT 
TTCTATCAGC CAGTTCGAGG TGAGGAGGAG AAAGCCTCTG AAACAGAGGT GCAGGCTCCG 
CAGATTCCCC AGATGGTGGC AAAGAGACCC CTGAGAGACT TTATTGGGCT GGAAGAATGC 
GATAAGACCA CAAGAGAAGC CATGCTCAAC TTCAGCTTTT ACCTCACAGT GGGAGATATG 
GATGAAGCAT TTAAGTCTAT CAAGCTTATT AAAAGTGAAG CAGTCTGGGA AAACATGGCT 
CGAATGTGCG TAAAGACCAA GAGGCTGGAT GTCGCCAAGG TTTGCCTAGG AAATATGGGC 
CATGCCAGGG GTGCCAAGGC CTTACGAGAG GCAGAACAGG AGCCAGAGGT GGAAGCGAGA 
GTGGCCATGC TGGCAATACA GCTGGGAATG TTGGAAGAAG CCGAGCAACT GTACAAAAGT 
TGCCTGCGAT ACGACCTCCT GAATAAGCTT TACCAAGCTT GGAACCAGTG GCAGAAGGCT 
ATTGAAACTG CAGAAAACCA TGACAGAATC CACCTGCGCT CCACCTACTA CAACTACGCC 
AAGCACCTAG AGGCCATTGG GGAACACAAT GAGTCACTCC TCTTGTATGA GAAATCTGAT 
ACTCACCGGT TTGAGGTTCC CAGAATGCTC CTGGATGACC CCCAGGCTTT GGAAGAGTAC 
ATCAATAAAA ATAAGGATAA GGGACTGTGG AAGTGGTGGG CACAATATTT GGAAAGCCAA 
TCAAACCTAG ACGCTGCGCT CAAATACTAT GAGATGGCTC AGGACTACCT GTCACTGGTC 
CGGGTGCACT GCTTCCTGGG GAATATCCAA AGGGCAGCAG AAATAGCGAA TGAGACAGGT 
AATGGGGCTG CATCCTACCA CCTTGCGCGT CATTATGAGA GCCAGGAAGA GGTGAAGCAA 
GCGGTGCACT TCTATACACG TGCCCAGGCA TATAACAATG GCATCAGGCT CTGCAAGGAG 
AACAACATGG ATGATCAGCT TATGAACCTT GCTCTGCTCA GCTCCCCTGA GGACATGATG 
GAGGCAGCTC GGTACTACGA AGAGAAGGGA GAGCAAATGG ATAGGGCAGT CATGCTCTAC 
CACAAGGCAG GACACTTCTC CAAGGCCTTG GAATTGGCTT TCACGACAGA GCAATTTGGG 
GCTTTGCAGC TGATTGCTGA GGATCTGGAT GAAAAGTCTG ACCCAGCCCT CCTTGCCCGT 
TGCTCTGACT TTTTCATGCA GCACAGCCAG TTTGAAAAGG CAGTGGAACT CCTGTTGGCA 
GCCAAGAAGT ATTATGAGGC ACTACAGCTC TGTATGGAAC AAAACCTTGT CATTACAGAA 
GAAATGGCTG AAAAAATGAC AGTGTCCAAA AATTCCAAGG ATTTTAGCGA CGAGGCCCGG 
CGTGAATTGC TGCAACGCAT TGCAGACTGC TGCATGAGGC AAGGGAACTA TCATCTTGCA 
ACCAAGAAAT ACACTCAGGC AGGGGATAAG TTAAAGGCTA TGAAGTCTTT GCTTAAGTCT 
GGCGACACAG AGAAGATTGT TTTCTTTGCT GGGGTGTCAC GTCAGAAAGA AATTTATATT 
ATGGCAGCCA ATTACCTGCA GTCCCTGGAC TGGCGTAAAG ATCCTGAGAT TATGAAGAAC 
ATCATCAGTT TCTACACTAA AGGCAGAGCC CTGGAACTGT TAGCTGGGTT TTATGATGCC 
TGTGCTCAGG TGGAGATTGA TGAATATCAG AGCTACGAAA AGGCGTTAGG AGCATTTACT 
GAGGCCTACA AGTGCTTGTC CAAAGCCAAA ATGCGAAGCC CAGAGGAGCA GGAGAGGAGG 
CTGTCTGACA TGCAAGCCAA GATGACTCTG GTGAAGAGGT TTATCCAGGC TCGCAGGGCA 
TACTCAGTGG ACAGTCAAGA AGCAGTAAAG CAATGTGAGC TGTTGCTAGA GGAGCCGGAT 
CTAGACAGCG CAATACGCAT CGGGGATGTG TATGGGCTAC TGGTGGAGCA CTATACTCAG 
CAGGGGGATT TTCAGAAGGC TTACCAGTGC CTTGAGGAAA TGCGCTCCAA GATGCCATCT 
GTCAACCTGA CTTACTACGT GCCCCAAGGT ACTATAGAAG CTGTTCACCG TGCCCTCTCT 
ATTCCATTTA ACCGCCATTC GCTGTCGGAG CACATGAGGC ACAATAGCGT GGAGGATAGC 
GAGGAGGTGG AGGAAGTACC GGAGATGGAC TATGGAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825483.1
CDS	363..4784
Translation	MAVYFDHRIQAPFPSASPSHISWHKHHALLAVGYISSGDGGSVDIYSELGDHVQDAQIKRTSHPTVLSWHPTKRTLVTGWTTGELVVFNEQDKEEYTLQTTHTAEITVLEWSSGNSRLVSADMSGTVAVWRLDQRGRIQVSPLLTNMYHKPLTHCLFKPLAPDEDLAQLAKAAVSGDEKALDMFNWNKSGKWTPMKSVSQQEFHFFLSAKDGSVYFVNEQLKNSQIVTLDSPVKRLFFMEKKSILVIVTENLLLSQFFISQEGNAEEISKVKLSGKTGQAADIHWVDNSLLITATGENVIRLWDLEREDNYVLSLDDQLGYDFAETLNCISYCSAKAILAAGTNKGRVAMWSRVTSHGQKAIRTDGKGTWKLQTPTELEENIMQIQWGSSKNFLAINNFSSVVVLSEQVMSAHFSQQVAAIQVAPTQLNLTYFSTETTHNLQTDMYIKGVFVTKDTIAVWNGKMVTVYELSGPTLRNTGSFSCESPVLVMHEDNIYTVETNRVQIWTLQGTVKQLLPFSEAEGNPCGLDICGHFLAVATDTAHFKIFDLSRREAKAHINCKNLSQMVPGVGSIVSVKCNVNGSKVSILANRSDRNLDSRMYFYDAELDTVTFYDFWSGQAEQGDSISNNDPDEHDAENRVLLDRIPINHFWDQSEPRLVVCEAMLAVPKSANTSPKHQSDQQTEIHQGKEDILVISLFSTQEHGLLIQESFPRPAALQSLLGIEVPHYYFTKKLFFRRGCFYQPVRGEEEKASETEVQAPQIPQMVAKRPLRDFIGLEECDKTTREAMLNFSFYLTVGDMDEAFKSIKLIKSEAVWENMARMCVKTKRLDVAKVCLGNMGHARGAKALREAEQEPEVEARVAMLAIQLGMLEEAEQLYKSCLRYDLLNKLYQAWNQWQKAIETAENHDRIHLRSTYYNYAKHLEAIGEHNESLLLYEKSDTHRFEVPRMLLDDPQALEEYINKNKDKGLWKWWAQYLESQSNLDAALKYYEMAQDYLSLVRVHCFLGNIQRAAEIANETGNGAASYHLARHYESQEEVKQAVHFYTRAQAYNNGIRLCKENNMDDQLMNLALLSSPEDMMEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTEQFGALQLIAEDLDEKSDPALLARCSDFFMQHSQFEKAVELLLAAKKYYEALQLCMEQNLVITEEMAEKMTVSKNSKDFSDEARRELLQRIADCCMRQGNYHLATKKYTQAGDKLKAMKSLLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKDPEIMKNIISFYTKGRALELLAGFYDACAQVEIDEYQSYEKALGAFTEAYKCLSKAKMRSPEEQERRLSDMQAKMTLVKRFIQARRAYSVDSQEAVKQCELLLEEPDLDSAIRIGDVYGLLVEHYTQQGDFQKAYQCLEEMRSKMPSVNLTYYVPQGTIEAVHRALSIPFNRHSLSEHMRHNSVEDSEEVEEVPEMDYGD

Target ORF information:

RefSeq Version	XM_012970029.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970029.2

ORF Insert Sequence:

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAG TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTAACAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA TATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGT TGTTTTTCCG GAGGGGTTGT 
TTCTATCAGC CAGTTCGAGG TGAGGAGGAG AAAGCCTCTG AAACAGAGGT GCAGGCTCCG 
CAGATTCCCC AGATGGTGGC AAAGAGACCC CTGAGAGACT TTATTGGGCT GGAAGAATGC 
GATAAGACCA CAAGAGAAGC CATGCTCAAC TTCAGCTTTT ACCTCACAGT GGGAGATATG 
GATGAAGCAT TTAAGTCTAT CAAGCTTATT AAAAGTGAAG CAGTCTGGGA AAACATGGCT 
CGAATGTGCG TAAAGACCAA GAGGCTGGAT GTCGCCAAGG TTTGCCTAGG AAATATGGGC 
CATGCCAGGG GTGCCAAGGC CTTACGAGAG GCAGAACAGG AGCCAGAGGT GGAAGCGAGA 
GTGGCCATGC TGGCAATACA GCTGGGAATG TTGGAAGAAG CCGAGCAACT GTACAAAAGT 
TGCCTGCGAT ACGACCTCCT GAATAAGCTT TACCAAGCTT GGAACCAGTG GCAGAAGGCT 
ATTGAAACTG CAGAAAACCA TGACAGAATC CACCTGCGCT CCACCTACTA CAACTACGCC 
AAGCACCTAG AGGCCATTGG GGAACACAAT GAGTCACTCC TCTTGTATGA GAAATCTGAT 
ACTCACCGGT TTGAGGTTCC CAGAATGCTC CTGGATGACC CCCAGGCTTT GGAAGAGTAC 
ATCAATAAAA ATAAGGATAA GGGACTGTGG AAGTGGTGGG CACAATATTT GGAAAGCCAA 
TCAAACCTAG ACGCTGCGCT CAAATACTAT GAGATGGCTC AGGACTACCT GTCACTGGTC 
CGGGTGCACT GCTTCCTGGG GAATATCCAA AGGGCAGCAG AAATAGCGAA TGAGACAGGT 
AATGGGGCTG CATCCTACCA CCTTGCGCGT CATTATGAGA GCCAGGAAGA GGTGAAGCAA 
GCGGTGCACT TCTATACACG TGCCCAGGCA TATAACAATG GCATCAGGCT CTGCAAGGAG 
AACAACATGG ATGATCAGCT TATGAACCTT GCTCTGCTCA GCTCCCCTGA GGACATGATG 
GAGGCAGCTC GGTACTACGA AGAGAAGGGA GAGCAAATGG ATAGGGCAGT CATGCTCTAC 
CACAAGGCAG GACACTTCTC CAAGGCCTTG GAATTGGCTT TCACGACAGA GCAATTTGGG 
GCTTTGCAGC TGATTGCTGA GGATCTGGAT GAAAAGTCTG ACCCAGCCCT CCTTGCCCGT 
TGCTCTGACT TTTTCATGCA GCACAGCCAG TTTGAAAAGG CAGTGGAACT CCTGTTGGCA 
GCCAAGAAGT ATTATGAGGC ACTACAGCTC TGTATGGAAC AAAACCTTGT CATTACAGAA 
GAAATGGCTG AAAAAATGAC AGTGTCCAAA AATTCCAAGG ATTTTAGCGA CGAGGCCCGG 
CGTGAATTGC TGCAACGCAT TGCAGACTGC TGCATGAGGC AAGGGAACTA TCATCTTGCA 
ACCAAGAAAT ACACTCAGGC AGGGGATAAG TTAAAGGCTA TGAAGTCTTT GCTTAAGTCT 
GGCGACACAG AGAAGATTGT TTTCTTTGCT GGGGTGTCAC GTCAGAAAGA AATTTATATT 
ATGGCAGCCA ATTACCTGCA GTCCCTGGAC TGGCGTAAAG ATCCTGAGAT TATGAAGAAC 
ATCATCAGTT TCTACACTAA AGGCAGAGCC CTGGAACTGT TAGCTGGGTT TTATGATGCC 
TGTGCTCAGG TGGAGATTGA TGAATATCAG AGCTACGAAA AGGCGTTAGG AGCATTTACT 
GAGGCCTACA AGTGCTTGTC CAAAGCCAAA ATGCGAAGCC CAGAGGAGCA GGAGAGGAGG 
CTGTCTGACA TGCAAGCCAA GATGACTCTG GTGAAGAGGT TTATCCAGGC TCGCAGGGCA 
TACTCAGTGG ACAGTCAAGA AGCAGTAAAG CAATGTGAGC TGTTGCTAGA GGAGCCGGAT 
CTAGACAGCG CAATACGCAT CGGGGATGTG TATGGGCTAC TGGTGGAGCA CTATACTCAG 
CAGGGGGATT TTCAGAAGGC TTACCAGTGC CTTGAGGAAA TGCGCTCCAA GATGCCATCT 
GTCAACCTGA CTTACTACGT GCCCCAAGGT ACTATAGAAG CTGTTCACCG TGCCCTCTCT 
ATTCCATTTA ACCGCCATTC GCTGTCGGAG CACATGAGGC ACAATAGCGT GGAGGATAGC 
GAGGAGGTGG AGGAAGTACC GGAGATGGAC TATGGAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ift140 ( XM_018097033.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952522.1 Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X2, mRNA.

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CloneID	OXa31338
Clone ID Related Accession (Same CDS sequence)	XM_012970030.2 , XM_012970031.2 , XM_012970029.2 , XM_018097033.1
Accession Version	XM_018097033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAG TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTAACAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA TATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGT TGTTTTTCCG GAGGGGTTGT 
TTCTATCAGC CAGTTCGAGG TGAGGAGGAG AAAGCCTCTG AAACAGAGGT GCAGGCTCCG 
CAGATTCCCC AGATGGTGGC AAAGAGACCC CTGAGAGACT TTATTGGGCT GGAAGAATGC 
GATAAGACCA CAAGAGAAGC CATGCTCAAC TTCAGCTTTT ACCTCACAGT GGGAGATATG 
GATGAAGCAT TTAAGTCTAT CAAGCTTATT AAAAGTGAAG CAGTCTGGGA AAACATGGCT 
CGAATGTGCG TAAAGACCAA GAGGCTGGAT GTCGCCAAGG TTTGCCTAGG AAATATGGGC 
CATGCCAGGG GTGCCAAGGC CTTACGAGAG GCAGAACAGG AGCCAGAGGT GGAAGCGAGA 
GTGGCCATGC TGGCAATACA GCTGGGAATG TTGGAAGAAG CCGAGCAACT GTACAAAAGT 
TGCCTGCGAT ACGACCTCCT GAATAAGCTT TACCAAGCTT GGAACCAGTG GCAGAAGGCT 
ATTGAAACTG CAGAAAACCA TGACAGAATC CACCTGCGCT CCACCTACTA CAACTACGCC 
AAGCACCTAG AGGCCATTGG GGAACACAAT GAGTCACTCC TCTTGTATGA GAAATCTGAT 
ACTCACCGGT TTGAGGTTCC CAGAATGCTC CTGGATGACC CCCAGGCTTT GGAAGAGTAC 
ATCAATAAAA ATAAGGATAA GGGACTGTGG AAGTGGTGGG CACAATATTT GGAAAGCCAA 
TCAAACCTAG ACGCTGCGCT CAAATACTAT GAGATGGCTC AGGACTACCT GTCACTGGTC 
CGGGTGCACT GCTTCCTGGG GAATATCCAA AGGGCAGCAG AAATAGCGAA TGAGACAGGT 
AATGGGGCTG CATCCTACCA CCTTGCGCGT CATTATGAGA GCCAGGAAGA GGTGAAGCAA 
GCGGTGCACT TCTATACACG TGCCCAGGCA TATAACAATG GCATCAGGCT CTGCAAGGAG 
AACAACATGG ATGATCAGCT TATGAACCTT GCTCTGCTCA GCTCCCCTGA GGACATGATG 
GAGGCAGCTC GGTACTACGA AGAGAAGGGA GAGCAAATGG ATAGGGCAGT CATGCTCTAC 
CACAAGGCAG GACACTTCTC CAAGGCCTTG GAATTGGCTT TCACGACAGA GCAATTTGGG 
GCTTTGCAGC TGATTGCTGA GGATCTGGAT GAAAAGTCTG ACCCAGCCCT CCTTGCCCGT 
TGCTCTGACT TTTTCATGCA GCACAGCCAG TTTGAAAAGG CAGTGGAACT CCTGTTGGCA 
GCCAAGAAGT ATTATGAGGC ACTACAGCTC TGTATGGAAC AAAACCTTGT CATTACAGAA 
GAAATGGCTG AAAAAATGAC AGTGTCCAAA AATTCCAAGG ATTTTAGCGA CGAGGCCCGG 
CGTGAATTGC TGCAACGCAT TGCAGACTGC TGCATGAGGC AAGGGAACTA TCATCTTGCA 
ACCAAGAAAT ACACTCAGGC AGGGGATAAG TTAAAGGCTA TGAAGTCTTT GCTTAAGTCT 
GGCGACACAG AGAAGATTGT TTTCTTTGCT GGGGTGTCAC GTCAGAAAGA AATTTATATT 
ATGGCAGCCA ATTACCTGCA GTCCCTGGAC TGGCGTAAAG ATCCTGAGAT TATGAAGAAC 
ATCATCAGTT TCTACACTAA AGGCAGAGCC CTGGAACTGT TAGCTGGGTT TTATGATGCC 
TGTGCTCAGG TGGAGATTGA TGAATATCAG AGCTACGAAA AGGCGTTAGG AGCATTTACT 
GAGGCCTACA AGTGCTTGTC CAAAGCCAAA ATGCGAAGCC CAGAGGAGCA GGAGAGGAGG 
CTGTCTGACA TGCAAGCCAA GATGACTCTG GTGAAGAGGT TTATCCAGGC TCGCAGGGCA 
TACTCAGTGG ACAGTCAAGA AGCAGTAAAG CAATGTGAGC TGTTGCTAGA GGAGCCGGAT 
CTAGACAGCG CAATACGCAT CGGGGATGTG TATGGGCTAC TGGTGGAGCA CTATACTCAG 
CAGGGGGATT TTCAGAAGGC TTACCAGTGC CTTGAGGAAA TGCGCTCCAA GATGCCATCT 
GTCAACCTGA CTTACTACGT GCCCCAAGGT ACTATAGAAG CTGTTCACCG TGCCCTCTCT 
ATTCCATTTA ACCGCCATTC GCTGTCGGAG CACATGAGGC ACAATAGCGT GGAGGATAGC 
GAGGAGGTGG AGGAAGTACC GGAGATGGAC TATGGAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952522.1
CDS	378..4799
Translation	MAVYFDHRIQAPFPSASPSHISWHKHHALLAVGYISSGDGGSVDIYSELGDHVQDAQIKRTSHPTVLSWHPTKRTLVTGWTTGELVVFNEQDKEEYTLQTTHTAEITVLEWSSGNSRLVSADMSGTVAVWRLDQRGRIQVSPLLTNMYHKPLTHCLFKPLAPDEDLAQLAKAAVSGDEKALDMFNWNKSGKWTPMKSVSQQEFHFFLSAKDGSVYFVNEQLKNSQIVTLDSPVKRLFFMEKKSILVIVTENLLLSQFFISQEGNAEEISKVKLSGKTGQAADIHWVDNSLLITATGENVIRLWDLEREDNYVLSLDDQLGYDFAETLNCISYCSAKAILAAGTNKGRVAMWSRVTSHGQKAIRTDGKGTWKLQTPTELEENIMQIQWGSSKNFLAINNFSSVVVLSEQVMSAHFSQQVAAIQVAPTQLNLTYFSTETTHNLQTDMYIKGVFVTKDTIAVWNGKMVTVYELSGPTLRNTGSFSCESPVLVMHEDNIYTVETNRVQIWTLQGTVKQLLPFSEAEGNPCGLDICGHFLAVATDTAHFKIFDLSRREAKAHINCKNLSQMVPGVGSIVSVKCNVNGSKVSILANRSDRNLDSRMYFYDAELDTVTFYDFWSGQAEQGDSISNNDPDEHDAENRVLLDRIPINHFWDQSEPRLVVCEAMLAVPKSANTSPKHQSDQQTEIHQGKEDILVISLFSTQEHGLLIQESFPRPAALQSLLGIEVPHYYFTKKLFFRRGCFYQPVRGEEEKASETEVQAPQIPQMVAKRPLRDFIGLEECDKTTREAMLNFSFYLTVGDMDEAFKSIKLIKSEAVWENMARMCVKTKRLDVAKVCLGNMGHARGAKALREAEQEPEVEARVAMLAIQLGMLEEAEQLYKSCLRYDLLNKLYQAWNQWQKAIETAENHDRIHLRSTYYNYAKHLEAIGEHNESLLLYEKSDTHRFEVPRMLLDDPQALEEYINKNKDKGLWKWWAQYLESQSNLDAALKYYEMAQDYLSLVRVHCFLGNIQRAAEIANETGNGAASYHLARHYESQEEVKQAVHFYTRAQAYNNGIRLCKENNMDDQLMNLALLSSPEDMMEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTEQFGALQLIAEDLDEKSDPALLARCSDFFMQHSQFEKAVELLLAAKKYYEALQLCMEQNLVITEEMAEKMTVSKNSKDFSDEARRELLQRIADCCMRQGNYHLATKKYTQAGDKLKAMKSLLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKDPEIMKNIISFYTKGRALELLAGFYDACAQVEIDEYQSYEKALGAFTEAYKCLSKAKMRSPEEQERRLSDMQAKMTLVKRFIQARRAYSVDSQEAVKQCELLLEEPDLDSAIRIGDVYGLLVEHYTQQGDFQKAYQCLEEMRSKMPSVNLTYYVPQGTIEAVHRALSIPFNRHSLSEHMRHNSVEDSEEVEEVPEMDYGD

Target ORF information:

RefSeq Version	XM_018097033.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis intraflagellar transport 140 (ift140), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097033.1

ORF Insert Sequence:

ATGGCTGTGT ATTTTGATCA CCGCATACAA GCTCCATTTC CTTCAGCGTC TCCATCACAC 
ATTTCCTGGC ACAAACACCA CGCTCTGCTT GCTGTAGGGT ACATCAGTTC AGGCGACGGA 
GGATCGGTGG ATATCTACAG TGAACTGGGT GATCATGTAC AAGATGCTCA AATAAAAAGA 
ACTTCCCATC CCACTGTGCT CAGCTGGCAT CCAACAAAAC GCACATTGGT GACAGGATGG 
ACCACTGGTG AACTTGTAGT CTTTAATGAA CAAGACAAGG AGGAGTACAC ATTGCAAACA 
ACGCATACTG CTGAGATCAC AGTGTTGGAA TGGAGCAGCG GTAACAGTCG ACTTGTCTCT 
GCAGATATGA GTGGGACAGT AGCAGTATGG AGGCTGGACC AGCGAGGACG GATTCAGGTG 
TCACCCTTGC TTACAAACAT GTATCACAAA CCTCTCACTC ACTGCCTCTT CAAGCCTTTA 
GCTCCTGATG AGGATCTTGC GCAGCTGGCG AAGGCTGCTG TCAGTGGAGA TGAGAAAGCA 
CTGGATATGT TCAATTGGAA CAAATCAGGG AAATGGACTC CCATGAAGTC TGTGTCTCAG 
CAGGAGTTTC ATTTCTTTCT CAGTGCAAAG GATGGCTCTG TGTACTTTGT AAATGAGCAG 
CTAAAGAACA GCCAGATTGT GACTCTGGAC AGCCCGGTGA AAAGGCTTTT CTTTATGGAA 
AAGAAATCCA TACTGGTTAT AGTCACAGAA AACCTGCTGC TGTCTCAGTT TTTTATCAGC 
CAAGAAGGAA ATGCTGAGGA GATCTCAAAG GTGAAGCTGA GTGGGAAGAC TGGTCAAGCC 
GCTGATATAC ATTGGGTTGA CAACAGCCTG CTGATCACAG CGACTGGGGA AAATGTTATT 
AGGTTGTGGG ATTTGGAGCG AGAAGACAAT TATGTGCTCT CACTAGATGA CCAGTTGGGG 
TACGACTTTG CAGAAACCCT GAACTGCATT TCTTACTGCA GTGCTAAAGC AATCTTGGCA 
GCTGGAACTA ACAAGGGCAG AGTGGCTATG TGGAGCAGAG TAACATCCCA TGGTCAAAAG 
GCCATAAGGA CTGATGGGAA GGGCACTTGG AAGCTTCAGA CTCCAACAGA ACTAGAAGAA 
AATATTATGC AGATACAGTG GGGCTCCTCA AAGAACTTCT TGGCTATCAA CAACTTCAGT 
TCAGTTGTGG TCCTCAGCGA GCAAGTCATG TCTGCACACT TCAGTCAACA AGTTGCAGCC 
ATCCAAGTTG CGCCCACCCA GCTCAATCTG ACCTATTTTA GCACTGAAAC GACTCACAAC 
CTGCAGACTG ACATGTACAT TAAAGGTGTT TTTGTTACCA AGGATACCAT TGCAGTTTGG 
AATGGTAAAA TGGTCACAGT GTATGAGCTA TCAGGACCGA CTCTGAGAAA CACAGGTTCC 
TTCTCATGTG AGTCTCCAGT GTTAGTTATG CATGAAGATA ATATATATAC AGTAGAAACT 
AACAGAGTCC AGATTTGGAC ACTGCAGGGC ACTGTGAAGC AGTTGCTGCC TTTTTCAGAA 
GCAGAGGGGA ATCCATGTGG TTTGGATATT TGCGGACACT TTCTAGCAGT TGCAACTGAT 
ACTGCTCATT TCAAGATCTT TGACCTTTCA CGGAGGGAAG CCAAAGCCCA TATCAACTGC 
AAAAACCTTT CCCAGATGGT TCCAGGAGTC GGGAGCATTG TGTCTGTGAA GTGTAATGTC 
AATGGCAGCA AAGTCAGCAT CCTTGCTAAT AGGTCGGACA GAAACCTGGA TTCCAGGATG 
TACTTCTATG ATGCTGAATT GGACACCGTG ACCTTTTATG ATTTTTGGAG CGGTCAAGCA 
GAACAAGGGG ACTCTATATC CAACAACGAC CCAGACGAGC ATGATGCAGA AAACAGAGTG 
TTACTTGACC GCATTCCAAT CAACCATTTC TGGGACCAGA GCGAACCTCG GCTTGTGGTG 
TGCGAGGCTA TGCTGGCAGT GCCAAAGTCT GCTAACACAT CACCAAAGCA TCAGTCTGAT 
CAGCAAACAG AGATTCATCA AGGAAAGGAG GACATCTTGG TGATCTCTTT ATTCAGCACC 
CAGGAGCATG GGCTTCTCAT TCAAGAGAGT TTCCCACGTC CTGCTGCCCT CCAGTCACTT 
CTGGGTATTG AGGTACCTCA CTACTATTTT ACAAAGAAGT TGTTTTTCCG GAGGGGTTGT 
TTCTATCAGC CAGTTCGAGG TGAGGAGGAG AAAGCCTCTG AAACAGAGGT GCAGGCTCCG 
CAGATTCCCC AGATGGTGGC AAAGAGACCC CTGAGAGACT TTATTGGGCT GGAAGAATGC 
GATAAGACCA CAAGAGAAGC CATGCTCAAC TTCAGCTTTT ACCTCACAGT GGGAGATATG 
GATGAAGCAT TTAAGTCTAT CAAGCTTATT AAAAGTGAAG CAGTCTGGGA AAACATGGCT 
CGAATGTGCG TAAAGACCAA GAGGCTGGAT GTCGCCAAGG TTTGCCTAGG AAATATGGGC 
CATGCCAGGG GTGCCAAGGC CTTACGAGAG GCAGAACAGG AGCCAGAGGT GGAAGCGAGA 
GTGGCCATGC TGGCAATACA GCTGGGAATG TTGGAAGAAG CCGAGCAACT GTACAAAAGT 
TGCCTGCGAT ACGACCTCCT GAATAAGCTT TACCAAGCTT GGAACCAGTG GCAGAAGGCT 
ATTGAAACTG CAGAAAACCA TGACAGAATC CACCTGCGCT CCACCTACTA CAACTACGCC 
AAGCACCTAG AGGCCATTGG GGAACACAAT GAGTCACTCC TCTTGTATGA GAAATCTGAT 
ACTCACCGGT TTGAGGTTCC CAGAATGCTC CTGGATGACC CCCAGGCTTT GGAAGAGTAC 
ATCAATAAAA ATAAGGATAA GGGACTGTGG AAGTGGTGGG CACAATATTT GGAAAGCCAA 
TCAAACCTAG ACGCTGCGCT CAAATACTAT GAGATGGCTC AGGACTACCT GTCACTGGTC 
CGGGTGCACT GCTTCCTGGG GAATATCCAA AGGGCAGCAG AAATAGCGAA TGAGACAGGT 
AATGGGGCTG CATCCTACCA CCTTGCGCGT CATTATGAGA GCCAGGAAGA GGTGAAGCAA 
GCGGTGCACT TCTATACACG TGCCCAGGCA TATAACAATG GCATCAGGCT CTGCAAGGAG 
AACAACATGG ATGATCAGCT TATGAACCTT GCTCTGCTCA GCTCCCCTGA GGACATGATG 
GAGGCAGCTC GGTACTACGA AGAGAAGGGA GAGCAAATGG ATAGGGCAGT CATGCTCTAC 
CACAAGGCAG GACACTTCTC CAAGGCCTTG GAATTGGCTT TCACGACAGA GCAATTTGGG 
GCTTTGCAGC TGATTGCTGA GGATCTGGAT GAAAAGTCTG ACCCAGCCCT CCTTGCCCGT 
TGCTCTGACT TTTTCATGCA GCACAGCCAG TTTGAAAAGG CAGTGGAACT CCTGTTGGCA 
GCCAAGAAGT ATTATGAGGC ACTACAGCTC TGTATGGAAC AAAACCTTGT CATTACAGAA 
GAAATGGCTG AAAAAATGAC AGTGTCCAAA AATTCCAAGG ATTTTAGCGA CGAGGCCCGG 
CGTGAATTGC TGCAACGCAT TGCAGACTGC TGCATGAGGC AAGGGAACTA TCATCTTGCA 
ACCAAGAAAT ACACTCAGGC AGGGGATAAG TTAAAGGCTA TGAAGTCTTT GCTTAAGTCT 
GGCGACACAG AGAAGATTGT TTTCTTTGCT GGGGTGTCAC GTCAGAAAGA AATTTATATT 
ATGGCAGCCA ATTACCTGCA GTCCCTGGAC TGGCGTAAAG ATCCTGAGAT TATGAAGAAC 
ATCATCAGTT TCTACACTAA AGGCAGAGCC CTGGAACTGT TAGCTGGGTT TTATGATGCC 
TGTGCTCAGG TGGAGATTGA TGAATATCAG AGCTACGAAA AGGCGTTAGG AGCATTTACT 
GAGGCCTACA AGTGCTTGTC CAAAGCCAAA ATGCGAAGCC CAGAGGAGCA GGAGAGGAGG 
CTGTCTGACA TGCAAGCCAA GATGACTCTG GTGAAGAGGT TTATCCAGGC TCGCAGGGCA 
TACTCAGTGG ACAGTCAAGA AGCAGTAAAG CAATGTGAGC TGTTGCTAGA GGAGCCGGAT 
CTAGACAGCG CAATACGCAT CGGGGATGTG TATGGGCTAC TGGTGGAGCA CTATACTCAG 
CAGGGGGATT TTCAGAAGGC TTACCAGTGC CTTGAGGAAA TGCGCTCCAA GATGCCATCT 
GTCAACCTGA CTTACTACGT GCCCCAAGGT ACTATAGAAG CTGTTCACCG TGCCCTCTCT 
ATTCCATTTA ACCGCCATTC GCTGTCGGAG CACATGAGGC ACAATAGCGT GGAGGATAGC 
GAGGAGGTGG AGGAAGTACC GGAGATGGAC TATGGAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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ift140 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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