ORF » Species Summary » Xenopus tropicalis » top3a cDNA ORF clone

top3a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following top3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa67140 XM_004918070.4
Latest version!		 Xenopus tropicalis DNA topoisomerase III alpha (top3a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa16491 XM_004918070.3
Latest version!		 Xenopus tropicalis topoisomerase (DNA) III alpha (top3a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    top3a ( XM_004918070.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918127.2 Xenopus tropicalis DNA topoisomerase III alpha (top3a), mRNA.
    Price & Availability↑
    CloneID OXa67140
    Clone ID Related Accession (Same CDS sequence) XM_004918070.4
    Accession Version XM_004918070.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3066bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 3-alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918070.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGTTATTT TGAGCAGGGT TCTAGCCCTC AGCAAGTTTA CGTTTGCAGC TCGTCCTGCA 
    GTTCGTTTCT GCTCTCATCG TGCCGCAGCA GAAATGGCCT TGAGGATCCC AGTTAATAAA 
    GTTCTGTGTG TGGCGGAAAA GAACGATGCT GCCAAAGGGA TTGCAGATCT TATGTCCAAT 
    AGCAGAATGC GGAGGAGGGA AGGTTTCTCC AAGTTTAACA AGATTTATGA ATATGAATAT 
    CATTTGTTTG GAAAGGACGT GACTGTGGTG ATGACTTCTG TATCAGGACA TCTGTTAGCT 
    CTCGACTTCC AGGACCACAT GAAAAAATGG CACAGCTGCA ACCCGGTGTC TCTGTTTGAG 
    GCAGAGGTGC AGAAGTACTG CCCTGATGAC TTTGTAAACA TAAAGCGGAC CCTGGAGCGG 
    GAGGTGCGAC AGTGTCAGGC TCTCATAATA TGGACTGATT GTGACAGAGA GGGAGAAAAC 
    ATTGGCTTTG AGATCATCCA TGTCTGCAAA TCAGTCAAGC CAAACTTGCA GGTTTTCCGG 
    GCCCGGTTCT CCGAGATCAC TCCTCGTTCC ATCCGGATGG CTTGTGAGCA CCTGGTACAA 
    CCTGACCAGA ACATCAGCGA CGCGGTAGAT GTACGAATGG AGCTTGACCT GCGGATAGGT 
    GCTGCTTTCA CAAGGTTCCA AACCCTCCGC CTTCAGAAGA TGTTTCCCTC TGTGCTGTCC 
    AATCAGCTCA TCAGCTACGG CAGCTGCCAG TTTCCCACCT TGGGGTTCGT GGTGGAGAGA 
    TTTAAAGCCA TCCAGGCTTT TATTCCTGAA ACCTTCTACA AGATTAAAGT GACTCATGAA 
    CACGAAGATG GACTGGTGGA GTTTAGCTGG AAACGGAACC GTCTGTTTAA CCACACGGCT 
    TGCCTGGTTC TCTATCAGAT CTGCATGGAG GATCCTATGG CTACTGTGAT GGAGGTGGGA 
    AGTAAACCAA AGAGCAAGTG GAGACCTGTG GCTTTAGACA CTGTGGAGCT GGAAAAGCTG 
    GCATCCCGCA AGCTGAAAAT TGGAGCCAAG GAAACCATGA AGGTGGCAGA GAAGCTTTAT 
    ACTCAAGGAT TCATCAGTTA TCCCCGCACA GAAACCAACA TTTTCCCCAA GGACCTTAAC 
    CTGACATCTC TGGTTGAGCA GCAGACGCAC GACCATCAGT GGGGAGGCTT CGCGCAGCGA 
    ATCTTGGAGA GGGGTGGGCC GACCCCACGG AACGGCAACA AGACAGATCA GGCTCACCCT 
    CCCATCCATC CCACTAAATA CACCAACAGT CTGCAGGGAA ATGAACAGCG GATCTACGAG 
    TTTATTGTGC GCCACTTCTT GGCATGTTGC TCCCAAGATG CACAGGGCCA GGAGACCACG 
    GTGGAGATCG ACATTGCTGC CGAGCGCTTT GTCGCTCATG GACTGATGAT CATCGCTAGG 
    AACTATCTAG ATGTCTATCC CTATGATAAA TGGAATACAA AGATCATCCC CGTCTATGAG 
    ATGGGCACCC GCTTTCAGCC CAGCACGGTG GAGATGGTGG ACGGGGAGAC CAGCCCCCCG 
    CAGCTTTTAA CAGAAGCCGA TCTCATTTCC CTGATGGAAA AGCATGGGAT TGGTACTGAT 
    GCCACCCACG CTGAGCACAT TGAGACCATC AAGTCGCGCA TGTATGTGGG TCTCACACCA 
    GAGCAGAGGT TTCTTCCTGG GGAGCTTGGA ATGGGGCTTG TGGAAGGGTA TGATTCTATG 
    GGCTTTGAAA TGTCAAAACC GGACTTGCGC GCTGAGCTGG AGTCGGACCT GAAGCTGATC 
    TGTGAAGGGA AGAAGGATAA ATTTTCAGTG CTGAGACTGC AGGTACAGAA GTACAAGCAG 
    GTCTTTGTGG AGGCAGCAGC CAAAGCCACC AAGTTGGACG AGGCCTTGTC TCAGTATTTT 
    GGCCAAGCAG CACAGCCGGT GCCACAGGAG GAAATCTATG CCGACACCCC ACTTCCTGTA 
    CGGAAATGCC CTCAGTGCAA CAAGGACATG GTGCTGAAGA CCAAGAGGGA TGGAGGGTTC 
    TTCCTTTCCT GCGTGGGGTA TCCCGGCTGT AAGTCGTCCG TCTGGTTCCC CAGCTTTGTC 
    TTGGAGGTCT CAAGGGATGA AAGTATCTGC GGCACGTGCA AGCCACACCC AGTGTACAAG 
    CTGAAGATGA AGTTTAAGCG CGGCAGTGTC CCGCCCCTCA TGCCGCTGGA GTTTGTCGGC 
    TGCATCGGCG GTTGCGACGA AACACTTCGG GAAATCCTGG ACCTGAAATA TCTGCATGGC 
    GGTAACAGGG CCCCCCAGGC AGCCCCACGA GAGAGCAACC ACTTGCAGGT GAACCAGACC 
    AGTAATCGTA TGGGGAACAG CGGGGATGGC CGAGGGCGCC AACCGCAGGC TAAAACTCTG 
    AAACCTCCTG TGGCCAAACC TTCTGCCTCC AGAGGAGTTG CCCCCACCCC CAGCAGCAGT 
    GAGAGCAATG AAGTGGTGTG TAATTGTGGT GTAGCGGCCG TGCAGCTGAC TGTACGTAAG 
    GAAGGACCCA ACCAGGGCCG GCCCTTCTAC AAGTGCAACG GCGGAGCCTG CAACTTTTTC 
    TTATGGGCGG ATCAGGCCGA TAGTGGCAGT CCGAATGCTC AGGGTGGGCA CACTACAGGG 
    CGTCCTTCCA ATGCCTTCAG CAACCCGAGG GCATCCGATA GCTTTAGGCC TGCAGGAGGG 
    GGCAGCGAGC CAGGATCTGA GGGGCAGGCA GTGTGTATGT GCAACCAACC CGCCGTCACC 
    CGTACTGTCC AGAAGGATGG CGCCAACAAA GGGCGGCAGT TCCATACCTG CTCCAAACCC 
    AGAGAGCAGC AGTGCGGCTT CTTCCAGTGG GCCGATGAGA ACGTTCCTCC AGCCTCCTCC 
    AGTGAATTCT CCGGTTCCTT CGGGGGAAGT TCATTCAGCG GAGGATCAGG GGCCCCACGA 
    GGCTTTGGGA AGAAAAACAA ACGTTCTGAG AGCAGTTTCT CGGGCCCAGT AGCAAAGAAG 
    CAGAGATCTT GTGGGATCTG CCACCAACCG GGGCACAACA GAACCAACTG CCCTCAGAAC 
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004918127.2
    CDS287..3352
    Translation

    Target ORF information:

    RefSeq Version XM_004918070.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase III alpha (top3a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004918070.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGTTATTT TGAGCAGGGT TCTAGCCCTC AGCAAGTTTA CGTTTGCAGC TCGTCCTGCA 
    GTTCGTTTCT GCTCTCATCG TGCCGCAGCA GAAATGGCCT TGAGGATCCC AGTTAATAAA 
    GTTCTGTGTG TGGCGGAAAA GAACGATGCT GCCAAAGGGA TTGCAGATCT TATGTCCAAT 
    AGCAGAATGC GGAGGAGGGA AGGTTTCTCC AAGTTTAACA AGATTTATGA ATATGAATAT 
    CATTTGTTTG GAAAGGACGT GACTGTGGTG ATGACTTCTG TATCAGGACA TCTGTTAGCT 
    CTCGACTTCC AGGACCACAT GAAAAAATGG CACAGCTGCA ACCCGGTGTC TCTGTTTGAG 
    GCAGAGGTGC AGAAGTACTG CCCTGATGAC TTTGTAAACA TAAAGCGGAC CCTGGAGCGG 
    GAGGTGCGAC AGTGTCAGGC TCTCATAATA TGGACTGATT GTGACAGAGA GGGAGAAAAC 
    ATTGGCTTTG AGATCATCCA TGTCTGCAAA TCAGTCAAGC CAAACTTGCA GGTTTTCCGG 
    GCCCGGTTCT CCGAGATCAC TCCTCGTTCC ATCCGGATGG CTTGTGAGCA CCTGGTACAA 
    CCTGACCAGA ACATCAGCGA CGCGGTAGAT GTACGAATGG AGCTTGACCT GCGGATAGGT 
    GCTGCTTTCA CAAGGTTCCA AACCCTCCGC CTTCAGAAGA TGTTTCCCTC TGTGCTGTCC 
    AATCAGCTCA TCAGCTACGG CAGCTGCCAG TTTCCCACCT TGGGGTTCGT GGTGGAGAGA 
    TTTAAAGCCA TCCAGGCTTT TATTCCTGAA ACCTTCTACA AGATTAAAGT GACTCATGAA 
    CACGAAGATG GACTGGTGGA GTTTAGCTGG AAACGGAACC GTCTGTTTAA CCACACGGCT 
    TGCCTGGTTC TCTATCAGAT CTGCATGGAG GATCCTATGG CTACTGTGAT GGAGGTGGGA 
    AGTAAACCAA AGAGCAAGTG GAGACCTGTG GCTTTAGACA CTGTGGAGCT GGAAAAGCTG 
    GCATCCCGCA AGCTGAAAAT TGGAGCCAAG GAAACCATGA AGGTGGCAGA GAAGCTTTAT 
    ACTCAAGGAT TCATCAGTTA TCCCCGCACA GAAACCAACA TTTTCCCCAA GGACCTTAAC 
    CTGACATCTC TGGTTGAGCA GCAGACGCAC GACCATCAGT GGGGAGGCTT CGCGCAGCGA 
    ATCTTGGAGA GGGGTGGGCC GACCCCACGG AACGGCAACA AGACAGATCA GGCTCACCCT 
    CCCATCCATC CCACTAAATA CACCAACAGT CTGCAGGGAA ATGAACAGCG GATCTACGAG 
    TTTATTGTGC GCCACTTCTT GGCATGTTGC TCCCAAGATG CACAGGGCCA GGAGACCACG 
    GTGGAGATCG ACATTGCTGC CGAGCGCTTT GTCGCTCATG GACTGATGAT CATCGCTAGG 
    AACTATCTAG ATGTCTATCC CTATGATAAA TGGAATACAA AGATCATCCC CGTCTATGAG 
    ATGGGCACCC GCTTTCAGCC CAGCACGGTG GAGATGGTGG ACGGGGAGAC CAGCCCCCCG 
    CAGCTTTTAA CAGAAGCCGA TCTCATTTCC CTGATGGAAA AGCATGGGAT TGGTACTGAT 
    GCCACCCACG CTGAGCACAT TGAGACCATC AAGTCGCGCA TGTATGTGGG TCTCACACCA 
    GAGCAGAGGT TTCTTCCTGG GGAGCTTGGA ATGGGGCTTG TGGAAGGGTA TGATTCTATG 
    GGCTTTGAAA TGTCAAAACC GGACTTGCGC GCTGAGCTGG AGTCGGACCT GAAGCTGATC 
    TGTGAAGGGA AGAAGGATAA ATTTTCAGTG CTGAGACTGC AGGTACAGAA GTACAAGCAG 
    GTCTTTGTGG AGGCAGCAGC CAAAGCCACC AAGTTGGACG AGGCCTTGTC TCAGTATTTT 
    GGCCAAGCAG CACAGCCGGT GCCACAGGAG GAAATCTATG CCGACACCCC ACTTCCTGTA 
    CGGAAATGCC CTCAGTGCAA CAAGGACATG GTGCTGAAGA CCAAGAGGGA TGGAGGGTTC 
    TTCCTTTCCT GCGTGGGGTA TCCCGGCTGT AAGTCGTCCG TCTGGTTCCC CAGCTTTGTC 
    TTGGAGGTCT CAAGGGATGA AAGTATCTGC GGCACGTGCA AGCCACACCC AGTGTACAAG 
    CTGAAGATGA AGTTTAAGCG CGGCAGTGTC CCGCCCCTCA TGCCGCTGGA GTTTGTCGGC 
    TGCATCGGCG GTTGCGACGA AACACTTCGG GAAATCCTGG ACCTGAAATA TCTGCATGGC 
    GGTAACAGGG CCCCCCAGGC AGCCCCACGA GAGAGCAACC ACTTGCAGGT GAACCAGACC 
    AGTAATCGTA TGGGGAACAG CGGGGATGGC CGAGGGCGCC AACCGCAGGC TAAAACTCTG 
    AAACCTCCTG TGGCCAAACC TTCTGCCTCC AGAGGAGTTG CCCCCACCCC CAGCAGCAGT 
    GAGAGCAATG AAGTGGTGTG TAATTGTGGT GTAGCGGCCG TGCAGCTGAC TGTACGTAAG 
    GAAGGACCCA ACCAGGGCCG GCCCTTCTAC AAGTGCAACG GCGGAGCCTG CAACTTTTTC 
    TTATGGGCGG ATCAGGCCGA TAGTGGCAGT CCGAATGCTC AGGGTGGGCA CACTACAGGG 
    CGTCCTTCCA ATGCCTTCAG CAACCCGAGG GCATCCGATA GCTTTAGGCC TGCAGGAGGG 
    GGCAGCGAGC CAGGATCTGA GGGGCAGGCA GTGTGTATGT GCAACCAACC CGCCGTCACC 
    CGTACTGTCC AGAAGGATGG CGCCAACAAA GGGCGGCAGT TCCATACCTG CTCCAAACCC 
    AGAGAGCAGC AGTGCGGCTT CTTCCAGTGG GCCGATGAGA ACGTTCCTCC AGCCTCCTCC 
    AGTGAATTCT CCGGTTCCTT CGGGGGAAGT TCATTCAGCG GAGGATCAGG GGCCCCACGA 
    GGCTTTGGGA AGAAAAACAA ACGTTCTGAG AGCAGTTTCT CGGGCCCAGT AGCAAAGAAG 
    CAGAGATCTT GTGGGATCTG CCACCAACCG GGGCACAACA GAACCAACTG CCCTCAGAAC 
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    top3a ( XM_004918070.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918127.1 Xenopus tropicalis topoisomerase (DNA) III alpha (top3a), mRNA.
    Price & Availability↑
    CloneID OXa16491
    Clone ID Related Accession (Same CDS sequence) XM_004918070.3
    Accession Version XM_004918070.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3066bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 3-alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918070.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGTTATTT TGAGCAGGGT TCTAGCCCTC AGCAAGTTTA CGTTTGCAGC TCGTCCTGCA 
    GTTCGTTTCT GCTCTCATCG TGCCGCAGCA GAAATGGCCT TGAGGATCCC AGTTAATAAA 
    GTTCTGTGTG TGGCGGAAAA GAACGATGCT GCCAAAGGGA TTGCAGATCT TATGTCCAAT 
    AGCAGAATGC GGAGGAGGGA AGGTTTCTCC AAGTTTAACA AGATTTATGA ATATGAATAT 
    CATTTGTTTG GGAAGGACGT GACTGTGGTG ATGACTTCTG TATCAGGACA TCTGTTAGCT 
    CTCGACTTCC AGGACCACAT GAAAAAATGG CACAGCTGCA ACCCGGTGTC TCTGTTTGAG 
    GCAGAAGTGC AGAAGTACTG CCCTGATGAC TTTGTAAACA TAAAGCGGAC CCTGGAGCGG 
    GAGGTGCGAC AGTGTCAGGC TCTCATAATA TGGACTGATT GTGACAGAGA GGGAGAAAAC 
    ATTGGCTTTG AGATCATCCA TGTCTGCAAA TCAGTCAAGC CAAACTTGCA GGTTTTCCGG 
    GCCCGGTTCT CCGAGATCAC TCCTCGTTCC ATCCGGATGG CTTGTGAGCA CCTGGTACAA 
    CCTGACCAGA ACATCAGCGA CGCGGTAGAT GTACGAATGG AGCTTGACCT GCGGATAGGT 
    GCTGCTTTCA CAAGGTTCCA AACCCTCCGC CTTCAGAAGA TGTTTCCCTC TGTGCTGTCC 
    AATCAGCTCA TCAGCTACGG CAGCTGCCAG TTTCCCACCT TGGGGTTCGT GGTGGAGAGA 
    TTTAAAGCCA TCCAGGCTTT TATTCCTGAA ACCTTCTACA AGATTAAAGT GACTCATGAA 
    CACGAAGATG GACTGGTGGA GTTTAGCTGG AAGCGGAACC GTCTGTTTAA CCACACGGCT 
    TGCCTGGTTC TCTATCAGAT CTGCATGGAG GATCCTATGG CTACTGTGAT GGAGGTGGGA 
    AGCAAACCAA AGAGCAAGTG GAGACCTGTG GCTTTAGACA CTGTGGAGCT GGAAAAGCTG 
    GCATCCCGCA AGCTGAAAAT TGGAGCCAAG GAAACCATGA AGGTGGCAGA GAAGCTTTAT 
    ACTCAAGGAT TCATCAGTTA TCCCCGCACA GAAACCAACA TTTTCCCCAA GGATCTTAAC 
    CTGACATCTC TGGTTGAGCA GCAGACGCAC GACCATCAGT GGGGAGGCTT CGCGCAGCGA 
    ATCTTGGAGA GGGGTGGGCC GACCCCACGG AACGGCAACA AGACAGATCA GGCTCACCCT 
    CCCATCCATC CCACTAAATA CACTAACAGT CTGCAGGGAA ATGAACAGCG GATCTACGAG 
    TTTATTGTGC GCCACTTCTT GGCATGTTGC TCCCAAGATG CACAGGGCCA GGAGACCACG 
    GTGGAGATCG ACATTGCTGC CGAGCGCTTT GTCGCTCATG GACTGATGAT CATCGCTAGG 
    AACTATCTAG ATGTCTATCC CTATGATAAA TGGAATACAA AGATCATCCC CGTCTATGAG 
    ATGGGCGCCC GCTTTCAGCC CAGCACGGTG GAGATGGTGG ACGGGGAGAC CAGCCCCCCG 
    CAGCTTTTAA CAGAAGCCGA TCTCATTTCC CTGATGGAAA AGCATGGGAT TGGTACTGAT 
    GCCACCCACG CTGAGCACAT TGAGACCATC AAGTCGCGCA TGTATGTGGG TCTCACACCA 
    GAGCAGAGGT TTCTTCCTGG GGAGCTTGGA ATGGGGCTTG TGGAAGGGTA TGATTCTATG 
    GGCTTTGAAA TGTCAAAACC GGACTTGCGC GCTGAGCTGG AGTCGGACCT GAAGCTGATC 
    TGTGAAGGGA AGAAGGATAA ATTTTCAGTG CTGAGACTGC AGGTACAGAA GTACAAGCAG 
    GTCTTTGTGG AGGCAGCAGC CAAAGCCACC AAGTTGGACG AGGCCTTGTC TCAGTATTTT 
    GGCCAAGCAG CACAGCCGGT GCCACAGGAG GAAATCTATG CCGACACCCC ACTTCCTGTA 
    CGGAAATGCC CTCAGTGCAA CAAGGACATG GTGCTGAAGA CCAAGAGGGA TGGAGGGTTC 
    TTCCTTTCCT GCGTGGGGTA TCCCGGCTGT AAGTCGTCCG TCTGGTTCCC CAGCTTTGTC 
    TTGGAGGTCT CAAGGGATGA AAGTATCTGC GGCACGTGCA AGCCACACCC AGTGTACAAG 
    CTGAAGATGA AGTTTAAGCG CGGCAGTGTT CCGCCCCTCA TGCCGCTGGA GTTTGTCGGC 
    TGCATCGGTG GTTGCGACGA AACACTTCGG GAAATCCTGG ACCTGAAATA TCTGCATGGC 
    GGTAACAGGG CCCCCCAGGC AGCCCCACGA GAGAGCAACC ACTTGCAGGT GAACCAGACC 
    AGTAATCGTA TGGGGAACAG CGGGGATGGC CGAGGGCGCC AACCGCAGGC TAAAACTCTG 
    AAACCTCCTG TGGCCAAACC TTCTGCCTCC AGAGGAGTTG CCCCCACCCC CAGCAGCAGT 
    GAGAGCAATG AAGTGGTGTG TAACTGTGGT GTAGCGGCCG TGCAGCTGAC TGTACGTAAG 
    GAAGGACCCA ACCAGGGCCG GCCCTTCTAC AAGTGCAACG GCGGAGCCTG CAACTTTTTC 
    CTATGGGCGG ATCAGGCTGA TAGCGGCAGT CCGAATGCTC AGGGTGGGCA CTCTACGGGG 
    CGCCCTTCCA ATGCCTTCAG CAACCCGAGG GCATCCGATA GCTTTAGGCC TGCAGGAGGG 
    GGCAGCGAGC CAGGATCTGA GGGGCAGGCG GTGTGTATGT GCAACCAACC CGCCGTCACC 
    CGTACTGTCC AGAAAGATGG CGCCAACAAA GGGCGGCAGT TCCATACCTG CTCCAAACCC 
    AGAGAGCAGC AGTGCGGCTT CTTCCAGTGG GCCGATGAGA ACGTTCCTCC AGCCTCCTCC 
    AGTGAATTCT CCGGTTCCTT TGGGGGAAGT TCATTCAGCG GAGGATCAGG GGCCCCACGA 
    GGCTTTGGGA AGAAAAACAA ACGTTCTGAG AGCAGTTTCT CGGGCCCAGT AGCAAAGAAG 
    CAGAGATCTT GTGGGATCTG CCACCAACCG GGGCACAACA GAACCAACTG CCCTCAGAAC 
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004918127.1
    CDS193..3258
    Translation

    Target ORF information:

    RefSeq Version XM_004918070.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) III alpha (top3a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004918070.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGTTATTT TGAGCAGGGT TCTAGCCCTC AGCAAGTTTA CGTTTGCAGC TCGTCCTGCA 
    GTTCGTTTCT GCTCTCATCG TGCCGCAGCA GAAATGGCCT TGAGGATCCC AGTTAATAAA 
    GTTCTGTGTG TGGCGGAAAA GAACGATGCT GCCAAAGGGA TTGCAGATCT TATGTCCAAT 
    AGCAGAATGC GGAGGAGGGA AGGTTTCTCC AAGTTTAACA AGATTTATGA ATATGAATAT 
    CATTTGTTTG GGAAGGACGT GACTGTGGTG ATGACTTCTG TATCAGGACA TCTGTTAGCT 
    CTCGACTTCC AGGACCACAT GAAAAAATGG CACAGCTGCA ACCCGGTGTC TCTGTTTGAG 
    GCAGAAGTGC AGAAGTACTG CCCTGATGAC TTTGTAAACA TAAAGCGGAC CCTGGAGCGG 
    GAGGTGCGAC AGTGTCAGGC TCTCATAATA TGGACTGATT GTGACAGAGA GGGAGAAAAC 
    ATTGGCTTTG AGATCATCCA TGTCTGCAAA TCAGTCAAGC CAAACTTGCA GGTTTTCCGG 
    GCCCGGTTCT CCGAGATCAC TCCTCGTTCC ATCCGGATGG CTTGTGAGCA CCTGGTACAA 
    CCTGACCAGA ACATCAGCGA CGCGGTAGAT GTACGAATGG AGCTTGACCT GCGGATAGGT 
    GCTGCTTTCA CAAGGTTCCA AACCCTCCGC CTTCAGAAGA TGTTTCCCTC TGTGCTGTCC 
    AATCAGCTCA TCAGCTACGG CAGCTGCCAG TTTCCCACCT TGGGGTTCGT GGTGGAGAGA 
    TTTAAAGCCA TCCAGGCTTT TATTCCTGAA ACCTTCTACA AGATTAAAGT GACTCATGAA 
    CACGAAGATG GACTGGTGGA GTTTAGCTGG AAGCGGAACC GTCTGTTTAA CCACACGGCT 
    TGCCTGGTTC TCTATCAGAT CTGCATGGAG GATCCTATGG CTACTGTGAT GGAGGTGGGA 
    AGCAAACCAA AGAGCAAGTG GAGACCTGTG GCTTTAGACA CTGTGGAGCT GGAAAAGCTG 
    GCATCCCGCA AGCTGAAAAT TGGAGCCAAG GAAACCATGA AGGTGGCAGA GAAGCTTTAT 
    ACTCAAGGAT TCATCAGTTA TCCCCGCACA GAAACCAACA TTTTCCCCAA GGATCTTAAC 
    CTGACATCTC TGGTTGAGCA GCAGACGCAC GACCATCAGT GGGGAGGCTT CGCGCAGCGA 
    ATCTTGGAGA GGGGTGGGCC GACCCCACGG AACGGCAACA AGACAGATCA GGCTCACCCT 
    CCCATCCATC CCACTAAATA CACTAACAGT CTGCAGGGAA ATGAACAGCG GATCTACGAG 
    TTTATTGTGC GCCACTTCTT GGCATGTTGC TCCCAAGATG CACAGGGCCA GGAGACCACG 
    GTGGAGATCG ACATTGCTGC CGAGCGCTTT GTCGCTCATG GACTGATGAT CATCGCTAGG 
    AACTATCTAG ATGTCTATCC CTATGATAAA TGGAATACAA AGATCATCCC CGTCTATGAG 
    ATGGGCGCCC GCTTTCAGCC CAGCACGGTG GAGATGGTGG ACGGGGAGAC CAGCCCCCCG 
    CAGCTTTTAA CAGAAGCCGA TCTCATTTCC CTGATGGAAA AGCATGGGAT TGGTACTGAT 
    GCCACCCACG CTGAGCACAT TGAGACCATC AAGTCGCGCA TGTATGTGGG TCTCACACCA 
    GAGCAGAGGT TTCTTCCTGG GGAGCTTGGA ATGGGGCTTG TGGAAGGGTA TGATTCTATG 
    GGCTTTGAAA TGTCAAAACC GGACTTGCGC GCTGAGCTGG AGTCGGACCT GAAGCTGATC 
    TGTGAAGGGA AGAAGGATAA ATTTTCAGTG CTGAGACTGC AGGTACAGAA GTACAAGCAG 
    GTCTTTGTGG AGGCAGCAGC CAAAGCCACC AAGTTGGACG AGGCCTTGTC TCAGTATTTT 
    GGCCAAGCAG CACAGCCGGT GCCACAGGAG GAAATCTATG CCGACACCCC ACTTCCTGTA 
    CGGAAATGCC CTCAGTGCAA CAAGGACATG GTGCTGAAGA CCAAGAGGGA TGGAGGGTTC 
    TTCCTTTCCT GCGTGGGGTA TCCCGGCTGT AAGTCGTCCG TCTGGTTCCC CAGCTTTGTC 
    TTGGAGGTCT CAAGGGATGA AAGTATCTGC GGCACGTGCA AGCCACACCC AGTGTACAAG 
    CTGAAGATGA AGTTTAAGCG CGGCAGTGTT CCGCCCCTCA TGCCGCTGGA GTTTGTCGGC 
    TGCATCGGTG GTTGCGACGA AACACTTCGG GAAATCCTGG ACCTGAAATA TCTGCATGGC 
    GGTAACAGGG CCCCCCAGGC AGCCCCACGA GAGAGCAACC ACTTGCAGGT GAACCAGACC 
    AGTAATCGTA TGGGGAACAG CGGGGATGGC CGAGGGCGCC AACCGCAGGC TAAAACTCTG 
    AAACCTCCTG TGGCCAAACC TTCTGCCTCC AGAGGAGTTG CCCCCACCCC CAGCAGCAGT 
    GAGAGCAATG AAGTGGTGTG TAACTGTGGT GTAGCGGCCG TGCAGCTGAC TGTACGTAAG 
    GAAGGACCCA ACCAGGGCCG GCCCTTCTAC AAGTGCAACG GCGGAGCCTG CAACTTTTTC 
    CTATGGGCGG ATCAGGCTGA TAGCGGCAGT CCGAATGCTC AGGGTGGGCA CTCTACGGGG 
    CGCCCTTCCA ATGCCTTCAG CAACCCGAGG GCATCCGATA GCTTTAGGCC TGCAGGAGGG 
    GGCAGCGAGC CAGGATCTGA GGGGCAGGCG GTGTGTATGT GCAACCAACC CGCCGTCACC 
    CGTACTGTCC AGAAAGATGG CGCCAACAAA GGGCGGCAGT TCCATACCTG CTCCAAACCC 
    AGAGAGCAGC AGTGCGGCTT CTTCCAGTGG GCCGATGAGA ACGTTCCTCC AGCCTCCTCC 
    AGTGAATTCT CCGGTTCCTT TGGGGGAAGT TCATTCAGCG GAGGATCAGG GGCCCCACGA 
    GGCTTTGGGA AGAAAAACAA ACGTTCTGAG AGCAGTTTCT CGGGCCCAGT AGCAAAGAAG 
    CAGAGATCTT GTGGGATCTG CCACCAACCG GGGCACAACA GAACCAACTG CCCTCAGAAC 
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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