egf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following egf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the egf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa22466 NM_001113007.1
Latest version!
Xenopus tropicalis epidermal growth factor (egf), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa30967 XM_012970125.2
Latest version!
Xenopus tropicalis epidermal growth factor (egf), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa22466
    Clone ID Related Accession (Same CDS sequence) NM_001113007.1
    Accession Version NM_001113007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3156bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pro-epidermal growth factor precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC154102.1. ##Evidence-Data-START## Transcript exon combination :: BC154102.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028291, SAMD00028292 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTTTTTT TATTGACTGC TCTCTGGCCA TTAGTTTTTG ATGTGCTTTT ACTTCATACC 
    CAAGGAAACA AACAATGGCA TTGTACAGGG AATCCACTTC CTTTGGATAA TGAACGCTAT
    GCATGTAACA CTCCTGTACC ATTCCTGATT TTTAGCCACG GCAATGACAT TTTTACCATT
    AACAGTGATG GGACCAATCT CAGGAAAGTA GTGCCCTCTG CAGGATCCAC ACTACAAATA
    GACTTTATCC AACAAGAGAA CAGACTTTAC TGGCTTGATC AAAACAGAGG TTTTCTTCAA
    AGAATGTACC TGAATGGCTC GAAGCATGAG AGGCTACGTG CTGTAGGAAA AGGGTCTATT
    GGATTTGCAA TTGACTGGAA ACACAATGTC ATTTTCTGGG CTAACCAACA GAAAGGAACA
    ATTGAACTGA CAAGCAGAAA TGAAAAAAGA TCTCGAGTAC TTCTGAGAGG TTTATTTAAT
    CCCAAGTTCG TTGCTATCGA TCCTTTGGAA GGGCTTCTCT TTTGGTCTTC TAACAAATCA
    TGGACTATTA TTCAAAAAGC AACTCTGGAT GGAAGTAATG TAACATCTAT TATTGAACTG
    AGTGGGGCAC TAAAAGGCCT AACACTGGAC ACAGATGACA AGAAGATTTT TTGGATCTTG
    TCTAAAATTG ACAGAGAGGA TTCTGTTATT GGCTCTTGTT CATATAATGG TGACTCTGTA
    ATGATTGTCA AGAATTTGAG ACTTTCAACG AAATATCATG TTTTGGGAGT TTCTCTTCTC
    TATAATCATA TCTATTATTC AGAGAGGAAA TCTGGTTCCA TAAAAGTAAT TAATAAATTC
    ACAGGGAAAG ATATTGTTTC AGTCAACTTG AAGCCATCAA TCATGGAGAT TAGAAATATA
    AAAGTAGTAT ATCCAGTGAG GTCCTATAGT ATTCCAAATT CCTCTTCCAA CCAAGGAGCA
    AAGTCGTGTT CCATTTCCAA TAAACACTGT ACAAAAACAT GTGAAATAGA TGCAGAGAAC
    CAGAAGTGCC ATTGTATGAA TGGTTTTGTC CTCAGTTCAA ATGGGCGATA TTGTGAAGAT
    ATAAATGAGT GTGCCCTATG GAATCATGGG TGTACTCTTG GATGTGAAAA TATCCCAGGT
    TCCTACTTTT GTACCTGCCC AGAAGGCTAT GTTTTACTTC CAGACAATAA GACATGCCAT
    GATAAAACTC CTTGCCTGAC AGAGCACATA GAATGTAGCC ATGGCTGTGT ACAAACAACC
    AAAGGGCCTT TGTGTACCTG TCCAGAAGGA TCGGTGCTCT CTGAAGATGG AAAGTCATGC
    ACAGGGTGCA CCTCCCCAGA TAATGGTGGA TGCAGCCATA TTTGCATAAT GTCAGGACCT
    GGGACTTGGG AATGTGACTG TTTTCCAGGG TACAACCTAC AGTTGGACAG AAAACAATGT
    TTGGCCTCAG GTCCTCGCCC ATATTTAATG TTTGCAAATG TTCATGACAT CAGACGAATT
    AACTTTGATG GAACCAATTA TGAAAGTGTA CTCGACTCAC AGATCGGTAG AGTGTTTGCC
    CTGGACTATG ACCCAGTTGA AGATAGGATG TACTTTGCAC ACACAGCTCT GAAGTGTATA
    GAAAGTGCAA ACATGGATGG CAGTGACCGG AAGAAAATGA TTTCTGGAGA TCTGAGCACA
    CTGGAAGGTC TTACTGTTGA TGCAATTAAT AGGAAACTAT ACTGGACTGA CCGAGGAAAA
    TCATGCATTG AAAGAAGTGA TTTAAATGGA AAATACAGGA AAATCCTCAT TCAAGAAAAT
    AACATTCAGC CGAGAGGAAT TTGTGTTCAT CCACTGGCTA AGAAATTATT TTGGACTCAT
    GTTGGAGCCA ACCCTCGTAT AGAGAGTTCG GATTTAGAAG GATCAGAAAG AGAGGTTATA
    ATCAGTAAGG ACCTGATCTG GCCCAGTGGA ATCACAGTCG ACCATCTCAC TGATGTTATT
    TATTGGTGCG ATGCCAAGAA ATCTGTTATC GAATCAGCTA ATCTGGATGG CTCAAATCGG
    CAGTCATTTT CCCAGAATGA TGTAGGCCAT CCTTTTGCCA TATCGGTCTT TGAGGATCAC
    TTGTGGGTCT CTGACTGGGT CCAGCCATCA CTTATAAGAA TTGAGAAAAG AAACACCCAG
    AACTGGGTAC GTCTTCGAGG CAACATGCAG AGACCCTCAT CTGTTGTAAT TGTACATCCT
    TTGGCAAAGC CAGTCATTGC AGATGACATC CCCCTGCCTA GAAAAGAGAA AGACAGCAAC
    AATCAAACAG CTCATTCTGA ATCTTTTTTT CCTGGTCCAC TTTCCACCAG CAATGCTGAA
    ATGGGGACGA GAGAAGCACC AAGTAGCAGA AATGGGTTGC TGGCAGAAAT TATGGTATCA
    GATGACAGTG GCTGCATTGA TATTCACTGT GATATCAATG CACATTGTGT CTCATCAGAG
    GATGGCCCAA AATGTCAGTG TTTGGAAGGC TTCACTGGGA ATGGGAGGTT ATGTCATGAT
    ATTGATGAAT GTTCTCTTCA CATTGCTGCG TGCAATCCCC ACCAGACAGA ATGCATTGAT
    ACAGAGGGGG GTTATATCTG CAAGTGCAAG TATGGCTACA TTGGAAATGG ACTTCAATGT
    ACAGATATTG ATGAATGCAG TCGGGGAACT CATACATGTG GGGAAAATGC AGTGTGCACC
    AATACAGAAG GGAATTACAC CTGCACATGC ACTAATGGCT TTCCAGGAAG CGCAGCCGAC
    TGTACTGGCA AGACAATAAT CAGAGAATGT CCCCTGGCTT ATGATGGATA CTGTCTCAAT
    GGAGGAGTGT GCATCCATTT CCCAGAACTG AAAGATTACG GGTGCAGATG TGTCGCAGGA
    TACGTTGGTG AGAGATGTCA ATTTGATGAT CTGAAGTCTT GGGAAAAACA TGTTACCCAA
    ATGAAGATTC ACAACGTTAC CATTGCTGTT TCCTTGCTCT TACTCCTACT GATCCTGGGC
    CTTGGCTCCT TTGCTATTTA TTATTACAGA CATCAAATGA CCCACAGAAA AAACCATTTT
    AAACAAGAAA CAGCACCGGG CACTACAGAA AAACCTATTT GCAGCACAAG TGGTCTATCA
    AGAAGCAGCG AGCCTGGGTG TTTAGGGAAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106478.1
    CDS353..3508
    Translation

    Target ORF information:

    RefSeq Version NM_001113007.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epidermal growth factor (egf), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001113007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTTTTTT TATTGACTGC TCTCTGGCCA TTAGTTTTTG ATGTGCTTTT ACTTCATACC 
    CAAGGAAACA AACAATGGCA TTGTACAGGG AATCCACTTC CTTTGGATAA TGAACGCTAT
    GCATGTAACA CTCCTGTACC ATTCCTGATT TTTAGCCACG GCAATGACAT TTTTACCATT
    AACAGTGATG GGACCAATCT CAGGAAAGTA GTGCCCTCTG CAGGATCCAC ACTACAAATA
    GACTTTATCC AACAAGAGAA CAGACTTTAC TGGCTTGATC AAAACAGAGG TTTTCTTCAA
    AGAATGTACC TGAATGGCTC GAAGCATGAG AGGCTACGTG CTGTAGGAAA AGGGTCTATT
    GGATTTGCAA TTGACTGGAA ACACAATGTC ATTTTCTGGG CTAACCAACA GAAAGGAACA
    ATTGAACTGA CAAGCAGAAA TGAAAAAAGA TCTCGAGTAC TTCTGAGAGG TTTATTTAAT
    CCCAAGTTCG TTGCTATCGA TCCTTTGGAA GGGCTTCTCT TTTGGTCTTC TAACAAATCA
    TGGACTATTA TTCAAAAAGC AACTCTGGAT GGAAGTAATG TAACATCTAT TATTGAACTG
    AGTGGGGCAC TAAAAGGCCT AACACTGGAC ACAGATGACA AGAAGATTTT TTGGATCTTG
    TCTAAAATTG ACAGAGAGGA TTCTGTTATT GGCTCTTGTT CATATAATGG TGACTCTGTA
    ATGATTGTCA AGAATTTGAG ACTTTCAACG AAATATCATG TTTTGGGAGT TTCTCTTCTC
    TATAATCATA TCTATTATTC AGAGAGGAAA TCTGGTTCCA TAAAAGTAAT TAATAAATTC
    ACAGGGAAAG ATATTGTTTC AGTCAACTTG AAGCCATCAA TCATGGAGAT TAGAAATATA
    AAAGTAGTAT ATCCAGTGAG GTCCTATAGT ATTCCAAATT CCTCTTCCAA CCAAGGAGCA
    AAGTCGTGTT CCATTTCCAA TAAACACTGT ACAAAAACAT GTGAAATAGA TGCAGAGAAC
    CAGAAGTGCC ATTGTATGAA TGGTTTTGTC CTCAGTTCAA ATGGGCGATA TTGTGAAGAT
    ATAAATGAGT GTGCCCTATG GAATCATGGG TGTACTCTTG GATGTGAAAA TATCCCAGGT
    TCCTACTTTT GTACCTGCCC AGAAGGCTAT GTTTTACTTC CAGACAATAA GACATGCCAT
    GATAAAACTC CTTGCCTGAC AGAGCACATA GAATGTAGCC ATGGCTGTGT ACAAACAACC
    AAAGGGCCTT TGTGTACCTG TCCAGAAGGA TCGGTGCTCT CTGAAGATGG AAAGTCATGC
    ACAGGGTGCA CCTCCCCAGA TAATGGTGGA TGCAGCCATA TTTGCATAAT GTCAGGACCT
    GGGACTTGGG AATGTGACTG TTTTCCAGGG TACAACCTAC AGTTGGACAG AAAACAATGT
    TTGGCCTCAG GTCCTCGCCC ATATTTAATG TTTGCAAATG TTCATGACAT CAGACGAATT
    AACTTTGATG GAACCAATTA TGAAAGTGTA CTCGACTCAC AGATCGGTAG AGTGTTTGCC
    CTGGACTATG ACCCAGTTGA AGATAGGATG TACTTTGCAC ACACAGCTCT GAAGTGTATA
    GAAAGTGCAA ACATGGATGG CAGTGACCGG AAGAAAATGA TTTCTGGAGA TCTGAGCACA
    CTGGAAGGTC TTACTGTTGA TGCAATTAAT AGGAAACTAT ACTGGACTGA CCGAGGAAAA
    TCATGCATTG AAAGAAGTGA TTTAAATGGA AAATACAGGA AAATCCTCAT TCAAGAAAAT
    AACATTCAGC CGAGAGGAAT TTGTGTTCAT CCACTGGCTA AGAAATTATT TTGGACTCAT
    GTTGGAGCCA ACCCTCGTAT AGAGAGTTCG GATTTAGAAG GATCAGAAAG AGAGGTTATA
    ATCAGTAAGG ACCTGATCTG GCCCAGTGGA ATCACAGTCG ACCATCTCAC TGATGTTATT
    TATTGGTGCG ATGCCAAGAA ATCTGTTATC GAATCAGCTA ATCTGGATGG CTCAAATCGG
    CAGTCATTTT CCCAGAATGA TGTAGGCCAT CCTTTTGCCA TATCGGTCTT TGAGGATCAC
    TTGTGGGTCT CTGACTGGGT CCAGCCATCA CTTATAAGAA TTGAGAAAAG AAACACCCAG
    AACTGGGTAC GTCTTCGAGG CAACATGCAG AGACCCTCAT CTGTTGTAAT TGTACATCCT
    TTGGCAAAGC CAGTCATTGC AGATGACATC CCCCTGCCTA GAAAAGAGAA AGACAGCAAC
    AATCAAACAG CTCATTCTGA ATCTTTTTTT CCTGGTCCAC TTTCCACCAG CAATGCTGAA
    ATGGGGACGA GAGAAGCACC AAGTAGCAGA AATGGGTTGC TGGCAGAAAT TATGGTATCA
    GATGACAGTG GCTGCATTGA TATTCACTGT GATATCAATG CACATTGTGT CTCATCAGAG
    GATGGCCCAA AATGTCAGTG TTTGGAAGGC TTCACTGGGA ATGGGAGGTT ATGTCATGAT
    ATTGATGAAT GTTCTCTTCA CATTGCTGCG TGCAATCCCC ACCAGACAGA ATGCATTGAT
    ACAGAGGGGG GTTATATCTG CAAGTGCAAG TATGGCTACA TTGGAAATGG ACTTCAATGT
    ACAGATATTG ATGAATGCAG TCGGGGAACT CATACATGTG GGGAAAATGC AGTGTGCACC
    AATACAGAAG GGAATTACAC CTGCACATGC ACTAATGGCT TTCCAGGAAG CGCAGCCGAC
    TGTACTGGCA AGACAATAAT CAGAGAATGT CCCCTGGCTT ATGATGGATA CTGTCTCAAT
    GGAGGAGTGT GCATCCATTT CCCAGAACTG AAAGATTACG GGTGCAGATG TGTCGCAGGA
    TACGTTGGTG AGAGATGTCA ATTTGATGAT CTGAAGTCTT GGGAAAAACA TGTTACCCAA
    ATGAAGATTC ACAACGTTAC CATTGCTGTT TCCTTGCTCT TACTCCTACT GATCCTGGGC
    CTTGGCTCCT TTGCTATTTA TTATTACAGA CATCAAATGA CCCACAGAAA AAACCATTTT
    AAACAAGAAA CAGCACCGGG CACTACAGAA AAACCTATTT GCAGCACAAG TGGTCTATCA
    AGAAGCAGCG AGCCTGGGTG TTTAGGGAAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30967
    Clone ID Related Accession (Same CDS sequence) XM_012970125.2
    Accession Version XM_012970125.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3033bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pro-epidermal growth factor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970125.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTTTTTT TATTGACTGC TCTCTGGCCA TTAGTTTTTG ATGTGCTTTT ACTTCATACC 
    CAAGGAAACA AACAATGGCA TTGTACAGGG AATCCACTTC CTTTGGATAA TGAACGCTAT
    GCATGTAACA CTCCTGTACC ATTCCTGATT TTTAGCCACG GCAATGACAT TTTTACCATT
    AACAGTGATG GGACCAATCT CAGGAAAGTA GTGCCCTCTG CAGGATCCAC ACTACAAATA
    GACTTTATCC AACAAGAGAA CAGACTTTAC TGGCTTGATC AAAACAGAGG TTTTCTTCAA
    AGAATGTACC TGAATGGCTC GAAGCATGAG AGGCTACGTG CTGTAGGAAA AGGGTCTATT
    GGATTTGCAA TTGACTGGAA ACACAATGTC ATTTTCTGGG CTAACCAACA GAAAGGAACA
    ATTGAACTGA CAAGCAGAAA TGAAAAAAGA TCTCGAGTAC TTCTGAGAGG TTTATTTAAT
    CCCAAGTTCG TTGCTATCGA TCCTTTGGAA GGGCTTCTCT TTTGGTCTTC TAACAAATCA
    TGGACTATTA TTCAAAAAGC AACTCTGGAT GGAAGTAATG TAACATCTAT TATTGAACTG
    AGTGGGGCAC TAAAAGGCCT AACACTGGAC ACAGATGACA AGAAGATTTT TTGGATCTTG
    TCTAAAATTG ACAGAGAGGA TTCTGTTATT GGCTCTTGTT CATATAATGG TGACTCTGTA
    ATGATTGTCA AGAATTTGAG ACTTTCAACG AAATATCATG TTTTGGGAGT TTCTCTTCTC
    TATAATCATA TCTATTATTC AGAGAGGAAA TCTGGTTCCA TAAAAGTAAT TAATAAATTC
    ACAGGGAAAG ATATTGTTTC AGTCAACTTG AAGCCATCAA TCATGGAGAT TAGAAATATA
    AAAGTAGTAT ATCCAGTGAG GTCCTATAGT ATTCCAAATT CCTCTTCCAA CCAAGATATA
    AATGAGTGTG CCCTATGGAA TCATGGGTGT ACTCTTGGAT GTGAAAATAT CCCAGGTTCC
    TACTTTTGTA CCTGCCCAGA AGGCTATGTT TTACTTCCAG ACAATAAGAC ATGCCATGAT
    AAAACTCCTT GCCTGACAGA GCACATAGAA TGTAGCCATG GCTGTGTACA AACAACCAAA
    GGGCCTTTGT GTACCTGTCC AGAAGGATCG GTGCTCTCTG AAGATGGAAA GTCATGCACA
    GGGTGCACCT CCCCAGATAA TGGTGGATGC AGCCATATTT GCATAATGTC AGGACCTGGG
    ACTTGGGAAT GTGACTGTTT TCCAGGGTAC AACCTACAGT TGGACAGAAA ACAATGTTTG
    GCCTCAGGTC CTCGCCCATA TTTAATGTTT GCAAATGTTC ATGACATCAG ACGAATTAAC
    TTTGATGGAA CCAATTATGA AAGTGTACTC GACTCACAGA TCGGTAGAGT GTTTGCCCTG
    GACTATGACC CAGTTGAAGA TAGGATGTAC TTTGCACACA CAGCTCTGAA GTGTATAGAA
    AGTGCAAACA TGGATGGCAG TGACCGGAAG AAAATGATTT CTGGAGATCT GAGCACACTG
    GAAGGTCTTA CTGTTGATGC AATTAATAGG AAACTATACT GGACTGACCG AGGAAAATCA
    TGCATTGAAA GAAGTGATTT AAATGGAAAA TACAGGAAAA TCCTCATTCA AGAAAATAAC
    ATTCAGCCGA GAGGAATTTG TGTTCATCCA CTGGCTAAGA AATTATTTTG GACTCATGTT
    GGAGCCAACC CTCGTATAGA GAGTTCGGAT TTAGAAGGAT CAGAAAGAGA GGTTATAATC
    AGTAAGGACC TGATCTGGCC CAGTGGAATC ACAGTCGACC ATCTCACTGA TGTTATTTAT
    TGGTGCGATG CCAAGAAATC TGTTATCGAA TCAGCTAATC TGGATGGCTC AAATCGGCAG
    TCATTTTCCC AGAATGATGT AGGCCATCCT TTTGCCATAT CAGTCTTTGA GGATCACTTG
    TGGGTCTCTG ACTGGGTCCA GCCATCACTT ATAAGAATTG AGAAAAGAAA CACCCAGAAC
    TGGGTACGTC TTCGAGGCAA CATGCAGAGA CCCTCATCTG TTGTAATTGT ACATCCTTTG
    GCAAAGCCAG TCATTGCAGA TGACATCCCC CTGCCTAGAA AAGAGAAAGA CAGCAACAAT
    CAAACAGCTC ATTCTGAATC TTTTTTTCCT GGTCCACTTT CCACCAGCAA CGCTGAAATG
    GGGACGAGAG AAGCACCAAG TAGCAGAAAT GGGTTGCTGG CAGAAATTAT GGTATCAGAT
    GACAGTGGCT GCATTGATAT TCACTGTGAT ATCAATGCAC ATTGTGTCTC ATCAGAGGAT
    GGCCCAAAAT GTCAGTGTTT GGAAGGCTTC ACTGGGAATG GGAGGTTATG TCATGATATT
    GATGAATGTT CTCTTCACAT TGCTGCGTGC AATCCCCACC AGACAGAATG CATTGATACA
    GAGGGGGGTT ATATCTGCAA GTGCAAGTAT GGCTACATTG GAAATGGACT TCAATGTACA
    GATATTGATG AATGCAGTCG GGGAACTCAT ACATGTGGGG AAAATGCAGT GTGCACCAAT
    ACAGAAGGGA ATTACACCTG CACATGCACT AATGGCTTTC CAGGAAGCGC AGCCGACTGT
    ACTGGCAAGA CAATAATCAG AGAATGTCCC CTGGCTTATG ATGGATACTG TCTCAATGGA
    GGAGTGTGCA TCCATTTCCC AGAACTGAAA GATTACGGGT GCAGATGTGT CGCAGGATAC
    GTTGGCGAGA GATGTCAATT TGATGATCTG AAGTCTTGGG AAAAACATGT TACCCAAATG
    AAGATTCACA ACGTTACCAT TGCTGTTTCC TTGCTCTTAC TCCTACTGAT CCTGGGCCTT
    GGCTCCTTTG CTACTTATTA TTACAGACAT CAAATGACCC ACAGAAAAAA CCATTTTAAA
    CAAGAAACAG CACCGGGCAC TACAGAAAAA CCTATTTGCA GCACAAGTGG TCTATCAAGA
    AGCAGCGAGC CTGGGTGTTT AGGGAAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825579.1
    CDS397..3429
    Translation

    Target ORF information:

    RefSeq Version XM_012970125.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epidermal growth factor (egf), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970125.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTTTTTT TATTGACTGC TCTCTGGCCA TTAGTTTTTG ATGTGCTTTT ACTTCATACC 
    CAAGGAAACA AACAATGGCA TTGTACAGGG AATCCACTTC CTTTGGATAA TGAACGCTAT
    GCATGTAACA CTCCTGTACC ATTCCTGATT TTTAGCCACG GCAATGACAT TTTTACCATT
    AACAGTGATG GGACCAATCT CAGGAAAGTA GTGCCCTCTG CAGGATCCAC ACTACAAATA
    GACTTTATCC AACAAGAGAA CAGACTTTAC TGGCTTGATC AAAACAGAGG TTTTCTTCAA
    AGAATGTACC TGAATGGCTC GAAGCATGAG AGGCTACGTG CTGTAGGAAA AGGGTCTATT
    GGATTTGCAA TTGACTGGAA ACACAATGTC ATTTTCTGGG CTAACCAACA GAAAGGAACA
    ATTGAACTGA CAAGCAGAAA TGAAAAAAGA TCTCGAGTAC TTCTGAGAGG TTTATTTAAT
    CCCAAGTTCG TTGCTATCGA TCCTTTGGAA GGGCTTCTCT TTTGGTCTTC TAACAAATCA
    TGGACTATTA TTCAAAAAGC AACTCTGGAT GGAAGTAATG TAACATCTAT TATTGAACTG
    AGTGGGGCAC TAAAAGGCCT AACACTGGAC ACAGATGACA AGAAGATTTT TTGGATCTTG
    TCTAAAATTG ACAGAGAGGA TTCTGTTATT GGCTCTTGTT CATATAATGG TGACTCTGTA
    ATGATTGTCA AGAATTTGAG ACTTTCAACG AAATATCATG TTTTGGGAGT TTCTCTTCTC
    TATAATCATA TCTATTATTC AGAGAGGAAA TCTGGTTCCA TAAAAGTAAT TAATAAATTC
    ACAGGGAAAG ATATTGTTTC AGTCAACTTG AAGCCATCAA TCATGGAGAT TAGAAATATA
    AAAGTAGTAT ATCCAGTGAG GTCCTATAGT ATTCCAAATT CCTCTTCCAA CCAAGATATA
    AATGAGTGTG CCCTATGGAA TCATGGGTGT ACTCTTGGAT GTGAAAATAT CCCAGGTTCC
    TACTTTTGTA CCTGCCCAGA AGGCTATGTT TTACTTCCAG ACAATAAGAC ATGCCATGAT
    AAAACTCCTT GCCTGACAGA GCACATAGAA TGTAGCCATG GCTGTGTACA AACAACCAAA
    GGGCCTTTGT GTACCTGTCC AGAAGGATCG GTGCTCTCTG AAGATGGAAA GTCATGCACA
    GGGTGCACCT CCCCAGATAA TGGTGGATGC AGCCATATTT GCATAATGTC AGGACCTGGG
    ACTTGGGAAT GTGACTGTTT TCCAGGGTAC AACCTACAGT TGGACAGAAA ACAATGTTTG
    GCCTCAGGTC CTCGCCCATA TTTAATGTTT GCAAATGTTC ATGACATCAG ACGAATTAAC
    TTTGATGGAA CCAATTATGA AAGTGTACTC GACTCACAGA TCGGTAGAGT GTTTGCCCTG
    GACTATGACC CAGTTGAAGA TAGGATGTAC TTTGCACACA CAGCTCTGAA GTGTATAGAA
    AGTGCAAACA TGGATGGCAG TGACCGGAAG AAAATGATTT CTGGAGATCT GAGCACACTG
    GAAGGTCTTA CTGTTGATGC AATTAATAGG AAACTATACT GGACTGACCG AGGAAAATCA
    TGCATTGAAA GAAGTGATTT AAATGGAAAA TACAGGAAAA TCCTCATTCA AGAAAATAAC
    ATTCAGCCGA GAGGAATTTG TGTTCATCCA CTGGCTAAGA AATTATTTTG GACTCATGTT
    GGAGCCAACC CTCGTATAGA GAGTTCGGAT TTAGAAGGAT CAGAAAGAGA GGTTATAATC
    AGTAAGGACC TGATCTGGCC CAGTGGAATC ACAGTCGACC ATCTCACTGA TGTTATTTAT
    TGGTGCGATG CCAAGAAATC TGTTATCGAA TCAGCTAATC TGGATGGCTC AAATCGGCAG
    TCATTTTCCC AGAATGATGT AGGCCATCCT TTTGCCATAT CAGTCTTTGA GGATCACTTG
    TGGGTCTCTG ACTGGGTCCA GCCATCACTT ATAAGAATTG AGAAAAGAAA CACCCAGAAC
    TGGGTACGTC TTCGAGGCAA CATGCAGAGA CCCTCATCTG TTGTAATTGT ACATCCTTTG
    GCAAAGCCAG TCATTGCAGA TGACATCCCC CTGCCTAGAA AAGAGAAAGA CAGCAACAAT
    CAAACAGCTC ATTCTGAATC TTTTTTTCCT GGTCCACTTT CCACCAGCAA CGCTGAAATG
    GGGACGAGAG AAGCACCAAG TAGCAGAAAT GGGTTGCTGG CAGAAATTAT GGTATCAGAT
    GACAGTGGCT GCATTGATAT TCACTGTGAT ATCAATGCAC ATTGTGTCTC ATCAGAGGAT
    GGCCCAAAAT GTCAGTGTTT GGAAGGCTTC ACTGGGAATG GGAGGTTATG TCATGATATT
    GATGAATGTT CTCTTCACAT TGCTGCGTGC AATCCCCACC AGACAGAATG CATTGATACA
    GAGGGGGGTT ATATCTGCAA GTGCAAGTAT GGCTACATTG GAAATGGACT TCAATGTACA
    GATATTGATG AATGCAGTCG GGGAACTCAT ACATGTGGGG AAAATGCAGT GTGCACCAAT
    ACAGAAGGGA ATTACACCTG CACATGCACT AATGGCTTTC CAGGAAGCGC AGCCGACTGT
    ACTGGCAAGA CAATAATCAG AGAATGTCCC CTGGCTTATG ATGGATACTG TCTCAATGGA
    GGAGTGTGCA TCCATTTCCC AGAACTGAAA GATTACGGGT GCAGATGTGT CGCAGGATAC
    GTTGGCGAGA GATGTCAATT TGATGATCTG AAGTCTTGGG AAAAACATGT TACCCAAATG
    AAGATTCACA ACGTTACCAT TGCTGTTTCC TTGCTCTTAC TCCTACTGAT CCTGGGCCTT
    GGCTCCTTTG CTACTTATTA TTACAGACAT CAAATGACCC ACAGAAAAAA CCATTTTAAA
    CAAGAAACAG CACCGGGCAC TACAGAAAAA CCTATTTGCA GCACAAGTGG TCTATCAAGA
    AGCAGCGAGC CTGGGTGTTT AGGGAAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.