brpf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	brpf1
Entrez Gene ID	100127652
Full Name	bromodomain and PHD finger containing 1
General protein information	Preferred Names bromodomain and PHD finger containing 1 Names peregrin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018092898.1	XP_017948387.1	peregrin isoform X2
NM_001112997.1	NP_001106468.1	peregrin
XM_012960522.2	XP_012815976.1	peregrin isoform X1
XM_012960522.3	XP_012815976.1	peregrin isoform X1
XM_012960523.3	XP_012815977.1	peregrin isoform X2
XM_012960523.2	XP_012815977.1	peregrin isoform X3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BRPF1	NP_001026635.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007617	XP_564621.3
Canis lupus familiaris (dog)	BRPF1	XP_850615.1
Mus musculus (house mouse)	Brpf1	NP_001269055.1
Danio rerio (zebrafish)	brpf1	NP_957310.1
Drosophila melanogaster (fruit fly)	Br140	NP_610266.1
Xenopus tropicalis (tropical clawed frog)	brpf1	NP_001106468.1
Pan troglodytes (chimpanzee)	BRPF1	XP_003309650.1
Bos taurus (cattle)	BRPF1	NP_001193018.1
Rattus norvegicus (Norway rat)	Brpf1	NP_001178501.1
Homo sapiens (human)	BRPF1	NP_001003694.1
	BRPF1	XP_001094039.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following brpf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brpf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa22269	NM_001112997.1 Latest version!	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa30956	XM_012960522.3 Latest version!	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa30957	XM_012960523.3 Latest version!	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa43710	XM_018092898.1 Latest version!	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OXa30956	XM_012960522.2	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa30957	XM_012960523.2	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brpf1 ( NM_001112997.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001106468.1 Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), mRNA.

Price & Availability↑

CloneID	OXa22269
Clone ID Related Accession (Same CDS sequence)	NM_001112997.1
Accession Version	NM_001112997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	peregrin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC154088.1. ##Evidence-Data-START## Transcript exon combination :: BC154088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028309, SAMN00862001 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGTTG ACTTTGATGT GAAGACATTT TGCCACAACT TGAGGGCCAC CAAGCCTCCC 
TATGAATGTC CGGTGGAGAC CTGCCGAAAG ATCTACAAGA GCTACAGTGG GATCGAGTAC 
CACCTGTATC ACTACGATCA TGACAACCCG CCTCTGCAGC AGCAGACTCC TATCCGCAAA 
TCAAAAAAGA AAGGCCGGCA CCCCCGATCC GCCAATAAGC AGTCACCGAC GAACTCGGAA 
GTGTCTCAGT CTCCGACCCG GGAGGTGATG ACTTACGCCC AGGCCCAGAG ACTGGTGGAG 
GTGGAATTGC ACGGGCGCGT GCATCGTATA AGTATATTTG ACAACTTGGA TGTTGTTTCT 
GAGGATGATG ACCCGCCCGA AGAAACCCCA GAAAATGGAA GTAATAAGGA AAATAATGAC 
ACCCCCGCGA CCACTCCTAA GTCGGGAAAG CACAAAAACA AGGATAAGAG AAAAGACTCC 
AACCATCATC ATCACCACAG TGCCACCGCA GGCGCCGCCA CCAAACTCCC AGAGGTTGTG 
TACCGAGAGC TCGAACTGGA CTCGCCAGAT GCTCCTCCGC GGCCTTCTTC CTATTACAGA 
CTCAGTAAGA TCACAAACCG CTTGACCATC CAGAGGAAGA GTCAGTTTAT GCAAAGGTTA 
CACAGCTACT GGACCTTAAA GAGACAGTCT CGGAATGGTG TCCCGCTTCT ACGTCGCCTG 
CAGACGCACC TACAGTCTCA AAGGAACTGC GACCAGGAGA TGAAGTGGCG GATACCAATT 
TCTGATGACA CGCCAGGTCA CCTTGGTACC ATCGCTATTT CTAACGAGGA GCCCGGAGAC 
CAAGCTAACA AGAATGACCC TACGGATAAG AACTGGGTGC TAAAGGAACA ATTAAAGTCA 
TGGCAGAGAC TTCGGCACGA CTTGGAGAGA GCTCGCCTTC TGGTAGAATT AATACGAAAG 
CGCGAGAAGC TCAAGAGAGA GACGATTAAA GTCCAGCAAA TGGCACTGGA AATGCAGCTG 
ACTCCATTTC TTATCCTACT GAGAAAAACC CTGGAGCTGC TGCAGGAGAA AGATACAAGT 
AATATATTCA CTGAGCCAGT CCCTCTGTCT GAGGTAACAG AAATCTACGA AGTGCCAGAC 
TACCTAGAAC ATGTTAAGAA GCCAATGGAC TTTCAGACCA TGAAGCGTAA CTTAGAGGCT 
TTCCGTTACC AGAATTTCGA CCAGTTTGAG GAAGACTTCA ACTTAATTGT GAACAATTGC 
ATAAAGTACA ACGCTAAAGA CACTATTTTT TACCGAGCCG CATCCCGCCT ACGAGAACAG 
GGAGCAGCCC TGCTGCGGCA GGCCCGCAAG CAGGCGGAAA AGATTGGCAT TGATTTCGAG 
ACGGGTATGC ATATACACCC AGTCATCACA GGAAATGAGA TTGGGCGCCA TGGCAGAGAG 
AATGACTTTG AGCGTGCTCA TCTGTCTGAC AGCCGCAAGC AACTCCCTTT AGAGGAGCAG 
CTGAAAGTCC AGTTGGCGAG ACTGGACGAG GTGAATGCAA GTAAGCAAAG CATAGGACGA 
GCGCGCCGGG CCAAGATGAT CCGCAAGGAG ATCACGTCGA TCCGTAGGAA GATGGTTTAC 
CAACAAGAAT CCAGAAGGGA CGGTGGCTCT GAGCGGCACA GCTCAGCCGG GAGAGGAGCC 
CTGCCTTTGC ACGGCCCTTC AGAGAAGGAT GGACAGACGG ACAGTGCTGC AGAAGAGAGC 
AGCAGCCAAG AGACCGGGAA AGGACTTGGC CCAAACACTT CCTCCACCAA TGCTCATGAA 
GTTGGTAGAA GGACTTCTGT CCTTTTCTCT AAGAAGAACC CCAAAACTGC AGGACCACCT 
AAGAGACCAG GAAGGCCACC TAAGAATAGA GACAATCAGC TCTCATCGGG ACAGATCAGC 
AGCCCCATTG GTCCCCCACA GCTGTCTGTC ACAGGGGGAT CTCAAAGGCG AAAGAGAGTC 
CGAAGCCCTC GGCCAACTTC AAGCTCTGAT AGCGACAGTG ACAGTGAAAA GTCAGCAGAG 
GAACCCTCTT TAGATTTGCC TACAAATGGC TTCAGCAGCA GCACCCAGCA AGTCAAAAAG 
AGTTTTTTGG TTTACCGGAC CGACTGCAAT CTTCCACGAA GCAGCTCAGA CTCTGAGTCC 
AGCACCACCA GCAGCAGCAG TGCTGCTTCC GACAGGACCA GCACAACTCC TTCCAAGCAG 
GGTCGGGGGA AGCCATCTTT CTCTAGGGAT AATTTTGCTG ACTACAGCAG TGAGGATACA 
TCTGGAACCG AGAATGAGTC TTACTCCGTT GGTGGGAGAG TCGGGCACGG CTTGGTACGC 
AAAGGACTGG AAAGCCGAGG GGCAGGGTGG CTGTCTGAAG ATGAAGATTC CCCTCTAGAA 
GCTTTAGACT TGGTGTGGGC CAAGTGTCGG GGCTATCCCT CCTATCCAGC TCTGATCATT 
GACCCAAAAA TGCCACGAGA AGGAGTGTTT CACCATGGCG TTCCGATTCC TGTGCCACCC 
GTGGATGTTC TCAAATTAGG AGAGCAGATG GCCCAAGAGG CACAAGAGCA TCTCTACCTC 
GTCCTGTTTT TTGACAACAA GAGAACCTGG CAGTGGCTTC CTCGGACAAA GTTAGTCCCT 
CTCGGCATGA ACCGGGAATT GGATAAGGAG AAGATGCTGG AAGGAAGAAA ATCAAACATC 
CGTAAATCTG TGCAGATCGC CTATGACCGT GCCATGCAGC ACCGTAACAA AGTACAGGGC 
GATCCAAGCA GTGAATCCAG TGAGAGCGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106468.1
CDS	176..2968
Translation	MGVDFDVKTFCHNLRATKPPYECPVETCRKIYKSYSGIEYHLYHYDHDNPPLQQQTPIRKSKKKGRHPRSANKQSPTNSEVSQSPTREVMTYAQAQRLVEVELHGRVHRISIFDNLDVVSEDDDPPEETPENGSNKENNDTPATTPKSGKHKNKDKRKDSNHHHHHSATAGAATKLPEVVYRELELDSPDAPPRPSSYYRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQRNCDQEMKWRIPISDDTPGHLGTIAISNEEPGDQANKNDPTDKNWVLKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKVQQMALEMQLTPFLILLRKTLELLQEKDTSNIFTEPVPLSEVTEIYEVPDYLEHVKKPMDFQTMKRNLEAFRYQNFDQFEEDFNLIVNNCIKYNAKDTIFYRAASRLREQGAALLRQARKQAEKIGIDFETGMHIHPVITGNEIGRHGRENDFERAHLSDSRKQLPLEEQLKVQLARLDEVNASKQSIGRARRAKMIRKEITSIRRKMVYQQESRRDGGSERHSSAGRGALPLHGPSEKDGQTDSAAEESSSQETGKGLGPNTSSTNAHEVGRRTSVLFSKKNPKTAGPPKRPGRPPKNRDNQLSSGQISSPIGPPQLSVTGGSQRRKRVRSPRPTSSSDSDSDSEKSAEEPSLDLPTNGFSSSTQQVKKSFLVYRTDCNLPRSSSDSESSTTSSSSAASDRTSTTPSKQGRGKPSFSRDNFADYSSEDTSGTENESYSVGGRVGHGLVRKGLESRGAGWLSEDEDSPLEALDLVWAKCRGYPSYPALIIDPKMPREGVFHHGVPIPVPPVDVLKLGEQMAQEAQEHLYLVLFFDNKRTWQWLPRTKLVPLGMNRELDKEKMLEGRKSNIRKSVQIAYDRAMQHRNKVQGDPSSESSESD

Target ORF information:

RefSeq Version	NM_001112997.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112997.1

ORF Insert Sequence:

ATGGGTGTTG ACTTTGATGT GAAGACATTT TGCCACAACT TGAGGGCCAC CAAGCCTCCC 
TATGAATGTC CGGTGGAGAC CTGCCGAAAG ATCTACAAGA GCTACAGTGG GATCGAGTAC 
CACCTGTATC ACTACGATCA TGACAACCCG CCTCTGCAGC AGCAGACTCC TATCCGCAAA 
TCAAAAAAGA AAGGCCGGCA CCCCCGATCC GCCAATAAGC AGTCACCGAC GAACTCGGAA 
GTGTCTCAGT CTCCGACCCG GGAGGTGATG ACTTACGCCC AGGCCCAGAG ACTGGTGGAG 
GTGGAATTGC ACGGGCGCGT GCATCGTATA AGTATATTTG ACAACTTGGA TGTTGTTTCT 
GAGGATGATG ACCCGCCCGA AGAAACCCCA GAAAATGGAA GTAATAAGGA AAATAATGAC 
ACCCCCGCGA CCACTCCTAA GTCGGGAAAG CACAAAAACA AGGATAAGAG AAAAGACTCC 
AACCATCATC ATCACCACAG TGCCACCGCA GGCGCCGCCA CCAAACTCCC AGAGGTTGTG 
TACCGAGAGC TCGAACTGGA CTCGCCAGAT GCTCCTCCGC GGCCTTCTTC CTATTACAGA 
CTCAGTAAGA TCACAAACCG CTTGACCATC CAGAGGAAGA GTCAGTTTAT GCAAAGGTTA 
CACAGCTACT GGACCTTAAA GAGACAGTCT CGGAATGGTG TCCCGCTTCT ACGTCGCCTG 
CAGACGCACC TACAGTCTCA AAGGAACTGC GACCAGGAGA TGAAGTGGCG GATACCAATT 
TCTGATGACA CGCCAGGTCA CCTTGGTACC ATCGCTATTT CTAACGAGGA GCCCGGAGAC 
CAAGCTAACA AGAATGACCC TACGGATAAG AACTGGGTGC TAAAGGAACA ATTAAAGTCA 
TGGCAGAGAC TTCGGCACGA CTTGGAGAGA GCTCGCCTTC TGGTAGAATT AATACGAAAG 
CGCGAGAAGC TCAAGAGAGA GACGATTAAA GTCCAGCAAA TGGCACTGGA AATGCAGCTG 
ACTCCATTTC TTATCCTACT GAGAAAAACC CTGGAGCTGC TGCAGGAGAA AGATACAAGT 
AATATATTCA CTGAGCCAGT CCCTCTGTCT GAGGTAACAG AAATCTACGA AGTGCCAGAC 
TACCTAGAAC ATGTTAAGAA GCCAATGGAC TTTCAGACCA TGAAGCGTAA CTTAGAGGCT 
TTCCGTTACC AGAATTTCGA CCAGTTTGAG GAAGACTTCA ACTTAATTGT GAACAATTGC 
ATAAAGTACA ACGCTAAAGA CACTATTTTT TACCGAGCCG CATCCCGCCT ACGAGAACAG 
GGAGCAGCCC TGCTGCGGCA GGCCCGCAAG CAGGCGGAAA AGATTGGCAT TGATTTCGAG 
ACGGGTATGC ATATACACCC AGTCATCACA GGAAATGAGA TTGGGCGCCA TGGCAGAGAG 
AATGACTTTG AGCGTGCTCA TCTGTCTGAC AGCCGCAAGC AACTCCCTTT AGAGGAGCAG 
CTGAAAGTCC AGTTGGCGAG ACTGGACGAG GTGAATGCAA GTAAGCAAAG CATAGGACGA 
GCGCGCCGGG CCAAGATGAT CCGCAAGGAG ATCACGTCGA TCCGTAGGAA GATGGTTTAC 
CAACAAGAAT CCAGAAGGGA CGGTGGCTCT GAGCGGCACA GCTCAGCCGG GAGAGGAGCC 
CTGCCTTTGC ACGGCCCTTC AGAGAAGGAT GGACAGACGG ACAGTGCTGC AGAAGAGAGC 
AGCAGCCAAG AGACCGGGAA AGGACTTGGC CCAAACACTT CCTCCACCAA TGCTCATGAA 
GTTGGTAGAA GGACTTCTGT CCTTTTCTCT AAGAAGAACC CCAAAACTGC AGGACCACCT 
AAGAGACCAG GAAGGCCACC TAAGAATAGA GACAATCAGC TCTCATCGGG ACAGATCAGC 
AGCCCCATTG GTCCCCCACA GCTGTCTGTC ACAGGGGGAT CTCAAAGGCG AAAGAGAGTC 
CGAAGCCCTC GGCCAACTTC AAGCTCTGAT AGCGACAGTG ACAGTGAAAA GTCAGCAGAG 
GAACCCTCTT TAGATTTGCC TACAAATGGC TTCAGCAGCA GCACCCAGCA AGTCAAAAAG 
AGTTTTTTGG TTTACCGGAC CGACTGCAAT CTTCCACGAA GCAGCTCAGA CTCTGAGTCC 
AGCACCACCA GCAGCAGCAG TGCTGCTTCC GACAGGACCA GCACAACTCC TTCCAAGCAG 
GGTCGGGGGA AGCCATCTTT CTCTAGGGAT AATTTTGCTG ACTACAGCAG TGAGGATACA 
TCTGGAACCG AGAATGAGTC TTACTCCGTT GGTGGGAGAG TCGGGCACGG CTTGGTACGC 
AAAGGACTGG AAAGCCGAGG GGCAGGGTGG CTGTCTGAAG ATGAAGATTC CCCTCTAGAA 
GCTTTAGACT TGGTGTGGGC CAAGTGTCGG GGCTATCCCT CCTATCCAGC TCTGATCATT 
GACCCAAAAA TGCCACGAGA AGGAGTGTTT CACCATGGCG TTCCGATTCC TGTGCCACCC 
GTGGATGTTC TCAAATTAGG AGAGCAGATG GCCCAAGAGG CACAAGAGCA TCTCTACCTC 
GTCCTGTTTT TTGACAACAA GAGAACCTGG CAGTGGCTTC CTCGGACAAA GTTAGTCCCT 
CTCGGCATGA ACCGGGAATT GGATAAGGAG AAGATGCTGG AAGGAAGAAA ATCAAACATC 
CGTAAATCTG TGCAGATCGC CTATGACCGT GCCATGCAGC ACCGTAACAA AGTACAGGGC 
GATCCAAGCA GTGAATCCAG TGAGAGCGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brpf1 ( XM_012960522.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815976.1 Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa30956
Clone ID Related Accession (Same CDS sequence)	XM_012960522.2 , XM_012960522.3
Accession Version	XM_012960522.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	peregrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960522.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG TTGACTTTGA TGTGAAGACA TTTTGCCACA ACTTGAGGGC CACCAAGCCT 
CCCTATGAAT GTCCGGTGGA GACCTGCCGA AAGATCTACA AGAGCTACAG TGGGATCGAG 
TACCACCTGT ATCACTACGA TCATGACAAC CCGCCTCTGC AGCAGCAGAC TCCTATCCGC 
AAATCAAAAA AGAAAGGCCG GCACCCCCGA TCCGCCAATA AGCAGTCACC GACGAACTCG 
GAAGTGTCTC AGTCTCCGAC CCGGGAGGTG ATGACTTACG CCCAGGCCCA GAGACTGGTG 
GAGGTGGAAT TGCACGGGCG CGTGCATCGT ATAAGTATAT TTGACAACTT GGATGTTGTT 
TCTGAGGATG ATGACCCGCC CGAAGAAACC CCAGAAAATG GAAGTAATAA GGAAAATAAT 
GACACCCCCG CGACCACTCC TAAGTCGGGA AAGCACAAAA ACAAGGATAA GAGAAAAGAC 
TCCAACCATC ATCATCACCA CAGTGCCACC GCAGGCGCCG CCACCAAACT CCCAGAGGTT 
GTGTACCGAG AGCTCGAACT GGACTCGCCA GATGCTCCTC CGCGGCCTTC TTCCTATTAC 
AGGTACATTG AGAAATCGGC AGAGGAACTA GACGAGGAGG TGGAGTATGA TATGGATGAG 
GAAGATTACA TCTGGCTTGA TATAATGAAT GATCGAAGGA AGAACGATGG AGTGAACCAT 
ATCCCACAAG AGATCTTTGA GTATCTAATG GACAGGTTGG AAAAGGAATC TTACTTTGAA 
AGCCATAATA AAGGGGACCC AAATTCTTTA ATTGACGAGG ATGCTGTATG CTGCATTTGC 
AACGATGGCG AGTGTCAGAA CAGCAATGTT ATATTGTTCT GCGATATGTG TAACCTGGCT 
GTGCACCAGG AGTGCTATGG GGTCCCTTAC ATACCAGAAG GACAATGGCT TTGTAGGAGG 
TGCCTCCAAT CACCTTCTAG GGCAGTGGAT TGTGCACTGT GCCCAAATAA GGGTGGGGCA 
TTCAAACAGA CAGATGATGG GCGCTGGGCA CACGTAGTTT GTGCCCTTTG GATTCCCGAG 
GTTTGTTTTG CAAACACAGT CTTCCTGGAA CCCATAGACA GCATTGAACA TATCCCACCT 
GCGCGTTGGA AACTGACATG CTATATCTGC AAACAGCGAG GCTCTGGAGC TTGCATCCAG 
TGCCACAAAG CAAACTGTTA TACAGCCTTC CATGTGACAT GCGCTCAGCA GGCTGGCCTG 
TACATGAAAA TGGAACCTGT GCGAGAGACT GGTGCTAACG GCACTTCTTT TAGTGTCCGC 
AAGACTGCGT ACTGTGATAT TCACACGCCT CCTGGGTCTG TTTGCAAGCT CCCAGCACTT 
TCTCATAGTG AAGGAGAGGA GGAGGATGAT GAGGAAGAAG AAGATGGCAA AGGATGGAGC 
TCTGAAAAAG TAAAAAAAGC AAAGGCCAAA TCAAGGATCA AAATGAAGAA AGCTCGGAAG 
ATCCTTGCTG AAAAGAGAGC TGCTGCCCCT GTTGTTTCTG TTCCTTGTAT CCCACCACAC 
AGACTCAGTA AGATCACAAA CCGCTTGACC ATCCAGAGGA AGAGTCAGTT TATGCAAAGG 
TTACACAGCT ACTGGACCTT AAAGAGACAG TCTCGGAATG GTGTCCCGCT TCTACGTCGC 
CTGCAGACGC ACCTACAGTC TCAAAGGAAC TGCGACCAGA AGGCAAGCTC CAGTGAGTCT 
GAGACGACCT ATTCAGCCTG CCTGTCTGCC TTTGGAACTG TACAGGAGAT GAAGTGGCGG 
ATACCAATTT CTGATGACAC GCCAGGTCAC CTTGGTACCA TCGCTATTTC TAACGAGGAG 
CCCGGAGACC AAGCTAACAA GAATGACCCT ACGGATAAGA ACTGGGTGCT AAAGGAACAA 
TTAAAGTCAT GGCAGAGACT TCGGCACGAC TTGGAGAGAG CTCGCCTTCT GGTAGAATTA 
ATACGAAAGC GCGAGAAGCT CAAGAGAGAG ACGATTAAAG TCCAGCAAAT GGCACTGGAA 
ATGCAGCTGA CTCCATTTCT TATCCTACTG AGAAAAACCC TGGAGCTGCT GCAGGAGAAA 
GATACAAGTA ATATATTCAC TGAGCCAGTC CCTCTGTCTG AGGTAACAGA AATCTACGAA 
GTGCCAGACT ACCTAGAACA TGTTAAGAAG CCAATGGACT TTCAGACCAT GAAGCGTAAC 
TTAGAGGCTT TCCGTTACCA GAATTTCGAC CAGTTTGAGG AAGACTTCAA CTTAATTGTG 
AACAATTGCA TAAAGTACAA CGCTAAAGAC ACTATTTTTT ACCGAGCCGC ATCCCGCCTA 
CGAGAACAGG GAGCAGCCCT GCTGCGGCAG GCCCGCAAGC AGGCGGAAAA GATTGGCATT 
GATTTCGAGA CGGGTATGCA TATACACCCA GTCATCACAG GAAATGAGAT TGGGCGCCAT 
GGCAGAGAGA ATGACTTTGA GCGTGCTCAT CTGTCTGACA GCCGCAAGCA ACTCCCTTTA 
GAGGAGCAGC TGAAAGTCCA GTTGGCGAGA CTGGATGAGG TGAATGCAAG TAAGCAAAGC 
ATAGGACGAG CGCGCCGGGC CAAGATGATC CGCAAGGAGA TCACGTCGAT CCGTAGGAAG 
ATGGTTTACC AACAAGAATC CAGAAGGGAC GGTGGCTCTG AGCGGCACAG CTCAGCCGGG 
AGAGGAGCCC TGCCTTTGCA CGGCCCTTCA GAGAAGGATG GACAGACGGA CAGTGCTGCA 
GAAGAGAGCA GCAGCCAAGA GACCGGGAAA GGACTTGGCC CAAACACTTC CTCCACCAAT 
GCTCATGAAG TTGGTAGAAG GACTTCTGTC CTTTTCTCTA AGAAGAACCC CAAAACTGCA 
GGACCACCTA AGAGACCAGG AAGGCCACCT AAGAATAGAG ACAATCAGCT CTCATCGGGA 
CAGATCAGCA GCCCCATTGG TCCCCCACAG CTGTCTGTCA CAGGGGGATC TCAAAGGCGA 
AAGAGAGTCC GAAGCCCTCG GCCAACTTCA AGCTCTGATA GCGACAGTGA CAGTGAAAAG 
TCAGCAGAGG AACCCTCTTT AGATTTGCCT ACAAATGGCT TCAGCAGCAG CACCCAGCAA 
GTCAAAAAGA GTTTTTTGGT TTACCGGACC GACTGCAATC TTCCACGAAG CAGCTCAGAC 
TCTGAGTCCA GCACCACCAG CAGCAGCAGT GCTGCTTCCG ACAGGACCAG CACAACTCCT 
TCCAAGCAGG GTCGGGGGAA GCCATCTTTC TCTAGGGATA ATTTTGCTGA CTACAGCAGT 
GAGGATACAT CTGGAACCGA GAATGAGTCT TACTCCGTTG GTGGGAGAGT CGGGCACGGC 
TTGGTACGCA AAGGACTGGA AAGCCGAGGG GCAGGGTGGC TGTCTGAAGA TGAAGATTCC 
CCTCTAGAAG CTTTAGACTT GGTGTGGGCC AAGTGTCGGG GCTATCCCTC CTATCCAGCT 
CTGATCATTG ACCCAAAAAT GCCACGAGAA GGAGTGTTTC ACCATGGCGT TCCGATTCCT 
GTGCCACCCG TGGATGTTCT CAAATTAGGA GAGCAGATGG CCCAAGAGGC ACAAGAGCAT 
CTCTACCTCG TCCTGTTTTT TGACAACAAG AGAACCTGGC AGTGGCTTCC TCGGACAAAG 
TTAGTCCCTC TCGGCATGAA CCGGGAATTG GATAAGGAGA AGATGCTGGA AGGAAGAAAA 
TCAAACATCC GCAAATCTGT GCAGATCGCC TATGACCGTG CCATGCAGCA CCGTAACAAA 
GTACAGGGCG ATCCAAGCAG TGAATCCAGT GAGAGCGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815976.1
CDS	251..4072
Translation	MMGVDFDVKTFCHNLRATKPPYECPVETCRKIYKSYSGIEYHLYHYDHDNPPLQQQTPIRKSKKKGRHPRSANKQSPTNSEVSQSPTREVMTYAQAQRLVEVELHGRVHRISIFDNLDVVSEDDDPPEETPENGSNKENNDTPATTPKSGKHKNKDKRKDSNHHHHHSATAGAATKLPEVVYRELELDSPDAPPRPSSYYRYIEKSAEELDEEVEYDMDEEDYIWLDIMNDRRKNDGVNHIPQEIFEYLMDRLEKESYFESHNKGDPNSLIDEDAVCCICNDGECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCPNKGGAFKQTDDGRWAHVVCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLYMKMEPVRETGANGTSFSVRKTAYCDIHTPPGSVCKLPALSHSEGEEEDDEEEEDGKGWSSEKVKKAKAKSRIKMKKARKILAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQRNCDQKASSSESETTYSACLSAFGTVQEMKWRIPISDDTPGHLGTIAISNEEPGDQANKNDPTDKNWVLKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKVQQMALEMQLTPFLILLRKTLELLQEKDTSNIFTEPVPLSEVTEIYEVPDYLEHVKKPMDFQTMKRNLEAFRYQNFDQFEEDFNLIVNNCIKYNAKDTIFYRAASRLREQGAALLRQARKQAEKIGIDFETGMHIHPVITGNEIGRHGRENDFERAHLSDSRKQLPLEEQLKVQLARLDEVNASKQSIGRARRAKMIRKEITSIRRKMVYQQESRRDGGSERHSSAGRGALPLHGPSEKDGQTDSAAEESSSQETGKGLGPNTSSTNAHEVGRRTSVLFSKKNPKTAGPPKRPGRPPKNRDNQLSSGQISSPIGPPQLSVTGGSQRRKRVRSPRPTSSSDSDSDSEKSAEEPSLDLPTNGFSSSTQQVKKSFLVYRTDCNLPRSSSDSESSTTSSSSAASDRTSTTPSKQGRGKPSFSRDNFADYSSEDTSGTENESYSVGGRVGHGLVRKGLESRGAGWLSEDEDSPLEALDLVWAKCRGYPSYPALIIDPKMPREGVFHHGVPIPVPPVDVLKLGEQMAQEAQEHLYLVLFFDNKRTWQWLPRTKLVPLGMNRELDKEKMLEGRKSNIRKSVQIAYDRAMQHRNKVQGDPSSESSESD

Target ORF information:

RefSeq Version	XM_012960522.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960522.3

ORF Insert Sequence:

ATGATGGGTG TTGACTTTGA TGTGAAGACA TTTTGCCACA ACTTGAGGGC CACCAAGCCT 
CCCTATGAAT GTCCGGTGGA GACCTGCCGA AAGATCTACA AGAGCTACAG TGGGATCGAG 
TACCACCTGT ATCACTACGA TCATGACAAC CCGCCTCTGC AGCAGCAGAC TCCTATCCGC 
AAATCAAAAA AGAAAGGCCG GCACCCCCGA TCCGCCAATA AGCAGTCACC GACGAACTCG 
GAAGTGTCTC AGTCTCCGAC CCGGGAGGTG ATGACTTACG CCCAGGCCCA GAGACTGGTG 
GAGGTGGAAT TGCACGGGCG CGTGCATCGT ATAAGTATAT TTGACAACTT GGATGTTGTT 
TCTGAGGATG ATGACCCGCC CGAAGAAACC CCAGAAAATG GAAGTAATAA GGAAAATAAT 
GACACCCCCG CGACCACTCC TAAGTCGGGA AAGCACAAAA ACAAGGATAA GAGAAAAGAC 
TCCAACCATC ATCATCACCA CAGTGCCACC GCAGGCGCCG CCACCAAACT CCCAGAGGTT 
GTGTACCGAG AGCTCGAACT GGACTCGCCA GATGCTCCTC CGCGGCCTTC TTCCTATTAC 
AGGTACATTG AGAAATCGGC AGAGGAACTA GACGAGGAGG TGGAGTATGA TATGGATGAG 
GAAGATTACA TCTGGCTTGA TATAATGAAT GATCGAAGGA AGAACGATGG AGTGAACCAT 
ATCCCACAAG AGATCTTTGA GTATCTAATG GACAGGTTGG AAAAGGAATC TTACTTTGAA 
AGCCATAATA AAGGGGACCC AAATTCTTTA ATTGACGAGG ATGCTGTATG CTGCATTTGC 
AACGATGGCG AGTGTCAGAA CAGCAATGTT ATATTGTTCT GCGATATGTG TAACCTGGCT 
GTGCACCAGG AGTGCTATGG GGTCCCTTAC ATACCAGAAG GACAATGGCT TTGTAGGAGG 
TGCCTCCAAT CACCTTCTAG GGCAGTGGAT TGTGCACTGT GCCCAAATAA GGGTGGGGCA 
TTCAAACAGA CAGATGATGG GCGCTGGGCA CACGTAGTTT GTGCCCTTTG GATTCCCGAG 
GTTTGTTTTG CAAACACAGT CTTCCTGGAA CCCATAGACA GCATTGAACA TATCCCACCT 
GCGCGTTGGA AACTGACATG CTATATCTGC AAACAGCGAG GCTCTGGAGC TTGCATCCAG 
TGCCACAAAG CAAACTGTTA TACAGCCTTC CATGTGACAT GCGCTCAGCA GGCTGGCCTG 
TACATGAAAA TGGAACCTGT GCGAGAGACT GGTGCTAACG GCACTTCTTT TAGTGTCCGC 
AAGACTGCGT ACTGTGATAT TCACACGCCT CCTGGGTCTG TTTGCAAGCT CCCAGCACTT 
TCTCATAGTG AAGGAGAGGA GGAGGATGAT GAGGAAGAAG AAGATGGCAA AGGATGGAGC 
TCTGAAAAAG TAAAAAAAGC AAAGGCCAAA TCAAGGATCA AAATGAAGAA AGCTCGGAAG 
ATCCTTGCTG AAAAGAGAGC TGCTGCCCCT GTTGTTTCTG TTCCTTGTAT CCCACCACAC 
AGACTCAGTA AGATCACAAA CCGCTTGACC ATCCAGAGGA AGAGTCAGTT TATGCAAAGG 
TTACACAGCT ACTGGACCTT AAAGAGACAG TCTCGGAATG GTGTCCCGCT TCTACGTCGC 
CTGCAGACGC ACCTACAGTC TCAAAGGAAC TGCGACCAGA AGGCAAGCTC CAGTGAGTCT 
GAGACGACCT ATTCAGCCTG CCTGTCTGCC TTTGGAACTG TACAGGAGAT GAAGTGGCGG 
ATACCAATTT CTGATGACAC GCCAGGTCAC CTTGGTACCA TCGCTATTTC TAACGAGGAG 
CCCGGAGACC AAGCTAACAA GAATGACCCT ACGGATAAGA ACTGGGTGCT AAAGGAACAA 
TTAAAGTCAT GGCAGAGACT TCGGCACGAC TTGGAGAGAG CTCGCCTTCT GGTAGAATTA 
ATACGAAAGC GCGAGAAGCT CAAGAGAGAG ACGATTAAAG TCCAGCAAAT GGCACTGGAA 
ATGCAGCTGA CTCCATTTCT TATCCTACTG AGAAAAACCC TGGAGCTGCT GCAGGAGAAA 
GATACAAGTA ATATATTCAC TGAGCCAGTC CCTCTGTCTG AGGTAACAGA AATCTACGAA 
GTGCCAGACT ACCTAGAACA TGTTAAGAAG CCAATGGACT TTCAGACCAT GAAGCGTAAC 
TTAGAGGCTT TCCGTTACCA GAATTTCGAC CAGTTTGAGG AAGACTTCAA CTTAATTGTG 
AACAATTGCA TAAAGTACAA CGCTAAAGAC ACTATTTTTT ACCGAGCCGC ATCCCGCCTA 
CGAGAACAGG GAGCAGCCCT GCTGCGGCAG GCCCGCAAGC AGGCGGAAAA GATTGGCATT 
GATTTCGAGA CGGGTATGCA TATACACCCA GTCATCACAG GAAATGAGAT TGGGCGCCAT 
GGCAGAGAGA ATGACTTTGA GCGTGCTCAT CTGTCTGACA GCCGCAAGCA ACTCCCTTTA 
GAGGAGCAGC TGAAAGTCCA GTTGGCGAGA CTGGATGAGG TGAATGCAAG TAAGCAAAGC 
ATAGGACGAG CGCGCCGGGC CAAGATGATC CGCAAGGAGA TCACGTCGAT CCGTAGGAAG 
ATGGTTTACC AACAAGAATC CAGAAGGGAC GGTGGCTCTG AGCGGCACAG CTCAGCCGGG 
AGAGGAGCCC TGCCTTTGCA CGGCCCTTCA GAGAAGGATG GACAGACGGA CAGTGCTGCA 
GAAGAGAGCA GCAGCCAAGA GACCGGGAAA GGACTTGGCC CAAACACTTC CTCCACCAAT 
GCTCATGAAG TTGGTAGAAG GACTTCTGTC CTTTTCTCTA AGAAGAACCC CAAAACTGCA 
GGACCACCTA AGAGACCAGG AAGGCCACCT AAGAATAGAG ACAATCAGCT CTCATCGGGA 
CAGATCAGCA GCCCCATTGG TCCCCCACAG CTGTCTGTCA CAGGGGGATC TCAAAGGCGA 
AAGAGAGTCC GAAGCCCTCG GCCAACTTCA AGCTCTGATA GCGACAGTGA CAGTGAAAAG 
TCAGCAGAGG AACCCTCTTT AGATTTGCCT ACAAATGGCT TCAGCAGCAG CACCCAGCAA 
GTCAAAAAGA GTTTTTTGGT TTACCGGACC GACTGCAATC TTCCACGAAG CAGCTCAGAC 
TCTGAGTCCA GCACCACCAG CAGCAGCAGT GCTGCTTCCG ACAGGACCAG CACAACTCCT 
TCCAAGCAGG GTCGGGGGAA GCCATCTTTC TCTAGGGATA ATTTTGCTGA CTACAGCAGT 
GAGGATACAT CTGGAACCGA GAATGAGTCT TACTCCGTTG GTGGGAGAGT CGGGCACGGC 
TTGGTACGCA AAGGACTGGA AAGCCGAGGG GCAGGGTGGC TGTCTGAAGA TGAAGATTCC 
CCTCTAGAAG CTTTAGACTT GGTGTGGGCC AAGTGTCGGG GCTATCCCTC CTATCCAGCT 
CTGATCATTG ACCCAAAAAT GCCACGAGAA GGAGTGTTTC ACCATGGCGT TCCGATTCCT 
GTGCCACCCG TGGATGTTCT CAAATTAGGA GAGCAGATGG CCCAAGAGGC ACAAGAGCAT 
CTCTACCTCG TCCTGTTTTT TGACAACAAG AGAACCTGGC AGTGGCTTCC TCGGACAAAG 
TTAGTCCCTC TCGGCATGAA CCGGGAATTG GATAAGGAGA AGATGCTGGA AGGAAGAAAA 
TCAAACATCC GCAAATCTGT GCAGATCGCC TATGACCGTG CCATGCAGCA CCGTAACAAA 
GTACAGGGCG ATCCAAGCAG TGAATCCAGT GAGAGCGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brpf1 ( XM_012960523.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815977.1 Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X2, mRNA.

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CloneID	OXa30957
Clone ID Related Accession (Same CDS sequence)	XM_012960523.3 , XM_012960523.2
Accession Version	XM_012960523.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	peregrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG TTGACTTTGA TGTGAAGACA TTTTGCCACA ACTTGAGGGC CACCAAGCCT 
CCCTATGAAT GTCCGGTGGA GACCTGCCGA AAGATCTACA AGAGCTACAG TGGGATCGAG 
TACCACCTGT ATCACTACGA TCATGACAAC CCGCCTCTGC AGCAGCAGAC TCCTATCCGC 
AAATCAAAAA AGAAAGGCCG GCACCCCCGA TCCGCCAATA AGCAGTCACC GACGAACTCG 
GAAGTGTCTC AGTCTCCGAC CCGGGAGGTG ATGACTTACG CCCAGGCCCA GAGACTGGTG 
GAGGTGGAAT TGCACGGGCG CGTGCATCGT ATAAGTATAT TTGACAACTT GGATGTTGTT 
TCTGAGGATG ATGACCCGCC CGAAGAAACC CCAGAAAATG GAAGTAATAA GGAAAATAAT 
GACACCCCCG CGACCACTCC TAAGTCGGGA AAGCACAAAA ACAAGGATAA GAGAAAAGAC 
TCCAACCATC ATCATCACCA CAGTGCCACC GCAGGCGCCG CCACCAAACT CCCAGAGGTT 
GTGTACCGAG AGCTCGAACT GGACTCGCCA GATGCTCCTC CGCGGCCTTC TTCCTATTAC 
AGGTACATTG AGAAATCGGC AGAGGAACTA GACGAGGAGG TGGAGTATGA TATGGATGAG 
GAAGATTACA TCTGGCTTGA TATAATGAAT GATCGAAGGA AGAACGATGG AGTGAACCAT 
ATCCCACAAG AGATCTTTGA GTATCTAATG GACAGGTTGG AAAAGGAATC TTACTTTGAA 
AGCCATAATA AAGGGGACCC AAATTCTTTA ATTGACGAGG ATGCTGTATG CTGCATTTGC 
AACGATGGCG AGTGTCAGAA CAGCAATGTT ATATTGTTCT GCGATATGTG TAACCTGGCT 
GTGCACCAGG AGTGCTATGG GGTCCCTTAC ATACCAGAAG GACAATGGCT TTGTAGGAGG 
TGCCTCCAAT CACCTTCTAG GGCAGTGGAT TGTGCACTGT GCCCAAATAA GGGTGGGGCA 
TTCAAACAGA CAGATGATGG GCGCTGGGCA CACGTAGTTT GTGCCCTTTG GATTCCCGAG 
GTTTGTTTTG CAAACACAGT CTTCCTGGAA CCCATAGACA GCATTGAACA TATCCCACCT 
GCGCGTTGGA AACTGACATG CTATATCTGC AAACAGCGAG GCTCTGGAGC TTGCATCCAG 
TGCCACAAAG CAAACTGTTA TACAGCCTTC CATGTGACAT GCGCTCAGCA GGCTGGCCTG 
TACATGAAAA TGGAACCTGT GCGAGAGACT GGTGCTAACG GCACTTCTTT TAGTGTCCGC 
AAGACTGCGT ACTGTGATAT TCACACGCCT CCTGGGTCTG TTTGCAAGCT CCCAGCACTT 
TCTCATAGTG AAGGAGAGGA GGAGGATGAT GAGGAAGAAG AAGATGGCAA AGGATGGAGC 
TCTGAAAAAG TAAAAAAAGC AAAGGCCAAA TCAAGGATCA AAATGAAGAA AGCTCGGAAG 
ATCCTTGCTG AAAAGAGAGC TGCTGCCCCT GTTGTTTCTG TTCCTTGTAT CCCACCACAC 
AGACTCAGTA AGATCACAAA CCGCTTGACC ATCCAGAGGA AGAGTCAGTT TATGCAAAGG 
TTACACAGCT ACTGGACCTT AAAGAGACAG TCTCGGAATG GTGTCCCGCT TCTACGTCGC 
CTGCAGACGC ACCTACAGTC TCAAAGGAAC TGCGACCAGG AGATGAAGTG GCGGATACCA 
ATTTCTGATG ACACGCCAGG TCACCTTGGT ACCATCGCTA TTTCTAACGA GGAGCCCGGA 
GACCAAGCTA ACAAGAATGA CCCTACGGAT AAGAACTGGG TGCTAAAGGA ACAATTAAAG 
TCATGGCAGA GACTTCGGCA CGACTTGGAG AGAGCTCGCC TTCTGGTAGA ATTAATACGA 
AAGCGCGAGA AGCTCAAGAG AGAGACGATT AAAGTCCAGC AAATGGCACT GGAAATGCAG 
CTGACTCCAT TTCTTATCCT ACTGAGAAAA ACCCTGGAGC TGCTGCAGGA GAAAGATACA 
AGTAATATAT TCACTGAGCC AGTCCCTCTG TCTGAGGTAA CAGAAATCTA CGAAGTGCCA 
GACTACCTAG AACATGTTAA GAAGCCAATG GACTTTCAGA CCATGAAGCG TAACTTAGAG 
GCTTTCCGTT ACCAGAATTT CGACCAGTTT GAGGAAGACT TCAACTTAAT TGTGAACAAT 
TGCATAAAGT ACAACGCTAA AGACACTATT TTTTACCGAG CCGCATCCCG CCTACGAGAA 
CAGGGAGCAG CCCTGCTGCG GCAGGCCCGC AAGCAGGCGG AAAAGATTGG CATTGATTTC 
GAGACGGGTA TGCATATACA CCCAGTCATC ACAGGAAATG AGATTGGGCG CCATGGCAGA 
GAGAATGACT TTGAGCGTGC TCATCTGTCT GACAGCCGCA AGCAACTCCC TTTAGAGGAG 
CAGCTGAAAG TCCAGTTGGC GAGACTGGAT GAGGTGAATG CAAGTAAGCA AAGCATAGGA 
CGAGCGCGCC GGGCCAAGAT GATCCGCAAG GAGATCACGT CGATCCGTAG GAAGATGGTT 
TACCAACAAG AATCCAGAAG GGACGGTGGC TCTGAGCGGC ACAGCTCAGC CGGGAGAGGA 
GCCCTGCCTT TGCACGGCCC TTCAGAGAAG GATGGACAGA CGGACAGTGC TGCAGAAGAG 
AGCAGCAGCC AAGAGACCGG GAAAGGACTT GGCCCAAACA CTTCCTCCAC CAATGCTCAT 
GAAGTTGGTA GAAGGACTTC TGTCCTTTTC TCTAAGAAGA ACCCCAAAAC TGCAGGACCA 
CCTAAGAGAC CAGGAAGGCC ACCTAAGAAT AGAGACAATC AGCTCTCATC GGGACAGATC 
AGCAGCCCCA TTGGTCCCCC ACAGCTGTCT GTCACAGGGG GATCTCAAAG GCGAAAGAGA 
GTCCGAAGCC CTCGGCCAAC TTCAAGCTCT GATAGCGACA GTGACAGTGA AAAGTCAGCA 
GAGGAACCCT CTTTAGATTT GCCTACAAAT GGCTTCAGCA GCAGCACCCA GCAAGTCAAA 
AAGAGTTTTT TGGTTTACCG GACCGACTGC AATCTTCCAC GAAGCAGCTC AGACTCTGAG 
TCCAGCACCA CCAGCAGCAG CAGTGCTGCT TCCGACAGGA CCAGCACAAC TCCTTCCAAG 
CAGGGTCGGG GGAAGCCATC TTTCTCTAGG GATAATTTTG CTGACTACAG CAGTGAGGAT 
ACATCTGGAA CCGAGAATGA GTCTTACTCC GTTGGTGGGA GAGTCGGGCA CGGCTTGGTA 
CGCAAAGGAC TGGAAAGCCG AGGGGCAGGG TGGCTGTCTG AAGATGAAGA TTCCCCTCTA 
GAAGCTTTAG ACTTGGTGTG GGCCAAGTGT CGGGGCTATC CCTCCTATCC AGCTCTGATC 
ATTGACCCAA AAATGCCACG AGAAGGAGTG TTTCACCATG GCGTTCCGAT TCCTGTGCCA 
CCCGTGGATG TTCTCAAATT AGGAGAGCAG ATGGCCCAAG AGGCACAAGA GCATCTCTAC 
CTCGTCCTGT TTTTTGACAA CAAGAGAACC TGGCAGTGGC TTCCTCGGAC AAAGTTAGTC 
CCTCTCGGCA TGAACCGGGA ATTGGATAAG GAGAAGATGC TGGAAGGAAG AAAATCAAAC 
ATCCGCAAAT CTGTGCAGAT CGCCTATGAC CGTGCCATGC AGCACCGTAA CAAAGTACAG 
GGCGATCCAA GCAGTGAATC CAGTGAGAGC GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815977.1
CDS	250..4005
Translation	MMGVDFDVKTFCHNLRATKPPYECPVETCRKIYKSYSGIEYHLYHYDHDNPPLQQQTPIRKSKKKGRHPRSANKQSPTNSEVSQSPTREVMTYAQAQRLVEVELHGRVHRISIFDNLDVVSEDDDPPEETPENGSNKENNDTPATTPKSGKHKNKDKRKDSNHHHHHSATAGAATKLPEVVYRELELDSPDAPPRPSSYYRYIEKSAEELDEEVEYDMDEEDYIWLDIMNDRRKNDGVNHIPQEIFEYLMDRLEKESYFESHNKGDPNSLIDEDAVCCICNDGECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCPNKGGAFKQTDDGRWAHVVCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLYMKMEPVRETGANGTSFSVRKTAYCDIHTPPGSVCKLPALSHSEGEEEDDEEEEDGKGWSSEKVKKAKAKSRIKMKKARKILAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQRNCDQEMKWRIPISDDTPGHLGTIAISNEEPGDQANKNDPTDKNWVLKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKVQQMALEMQLTPFLILLRKTLELLQEKDTSNIFTEPVPLSEVTEIYEVPDYLEHVKKPMDFQTMKRNLEAFRYQNFDQFEEDFNLIVNNCIKYNAKDTIFYRAASRLREQGAALLRQARKQAEKIGIDFETGMHIHPVITGNEIGRHGRENDFERAHLSDSRKQLPLEEQLKVQLARLDEVNASKQSIGRARRAKMIRKEITSIRRKMVYQQESRRDGGSERHSSAGRGALPLHGPSEKDGQTDSAAEESSSQETGKGLGPNTSSTNAHEVGRRTSVLFSKKNPKTAGPPKRPGRPPKNRDNQLSSGQISSPIGPPQLSVTGGSQRRKRVRSPRPTSSSDSDSDSEKSAEEPSLDLPTNGFSSSTQQVKKSFLVYRTDCNLPRSSSDSESSTTSSSSAASDRTSTTPSKQGRGKPSFSRDNFADYSSEDTSGTENESYSVGGRVGHGLVRKGLESRGAGWLSEDEDSPLEALDLVWAKCRGYPSYPALIIDPKMPREGVFHHGVPIPVPPVDVLKLGEQMAQEAQEHLYLVLFFDNKRTWQWLPRTKLVPLGMNRELDKEKMLEGRKSNIRKSVQIAYDRAMQHRNKVQGDPSSESSESD

Target ORF information:

RefSeq Version	XM_012960523.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960523.3

ORF Insert Sequence:

ATGATGGGTG TTGACTTTGA TGTGAAGACA TTTTGCCACA ACTTGAGGGC CACCAAGCCT 
CCCTATGAAT GTCCGGTGGA GACCTGCCGA AAGATCTACA AGAGCTACAG TGGGATCGAG 
TACCACCTGT ATCACTACGA TCATGACAAC CCGCCTCTGC AGCAGCAGAC TCCTATCCGC 
AAATCAAAAA AGAAAGGCCG GCACCCCCGA TCCGCCAATA AGCAGTCACC GACGAACTCG 
GAAGTGTCTC AGTCTCCGAC CCGGGAGGTG ATGACTTACG CCCAGGCCCA GAGACTGGTG 
GAGGTGGAAT TGCACGGGCG CGTGCATCGT ATAAGTATAT TTGACAACTT GGATGTTGTT 
TCTGAGGATG ATGACCCGCC CGAAGAAACC CCAGAAAATG GAAGTAATAA GGAAAATAAT 
GACACCCCCG CGACCACTCC TAAGTCGGGA AAGCACAAAA ACAAGGATAA GAGAAAAGAC 
TCCAACCATC ATCATCACCA CAGTGCCACC GCAGGCGCCG CCACCAAACT CCCAGAGGTT 
GTGTACCGAG AGCTCGAACT GGACTCGCCA GATGCTCCTC CGCGGCCTTC TTCCTATTAC 
AGGTACATTG AGAAATCGGC AGAGGAACTA GACGAGGAGG TGGAGTATGA TATGGATGAG 
GAAGATTACA TCTGGCTTGA TATAATGAAT GATCGAAGGA AGAACGATGG AGTGAACCAT 
ATCCCACAAG AGATCTTTGA GTATCTAATG GACAGGTTGG AAAAGGAATC TTACTTTGAA 
AGCCATAATA AAGGGGACCC AAATTCTTTA ATTGACGAGG ATGCTGTATG CTGCATTTGC 
AACGATGGCG AGTGTCAGAA CAGCAATGTT ATATTGTTCT GCGATATGTG TAACCTGGCT 
GTGCACCAGG AGTGCTATGG GGTCCCTTAC ATACCAGAAG GACAATGGCT TTGTAGGAGG 
TGCCTCCAAT CACCTTCTAG GGCAGTGGAT TGTGCACTGT GCCCAAATAA GGGTGGGGCA 
TTCAAACAGA CAGATGATGG GCGCTGGGCA CACGTAGTTT GTGCCCTTTG GATTCCCGAG 
GTTTGTTTTG CAAACACAGT CTTCCTGGAA CCCATAGACA GCATTGAACA TATCCCACCT 
GCGCGTTGGA AACTGACATG CTATATCTGC AAACAGCGAG GCTCTGGAGC TTGCATCCAG 
TGCCACAAAG CAAACTGTTA TACAGCCTTC CATGTGACAT GCGCTCAGCA GGCTGGCCTG 
TACATGAAAA TGGAACCTGT GCGAGAGACT GGTGCTAACG GCACTTCTTT TAGTGTCCGC 
AAGACTGCGT ACTGTGATAT TCACACGCCT CCTGGGTCTG TTTGCAAGCT CCCAGCACTT 
TCTCATAGTG AAGGAGAGGA GGAGGATGAT GAGGAAGAAG AAGATGGCAA AGGATGGAGC 
TCTGAAAAAG TAAAAAAAGC AAAGGCCAAA TCAAGGATCA AAATGAAGAA AGCTCGGAAG 
ATCCTTGCTG AAAAGAGAGC TGCTGCCCCT GTTGTTTCTG TTCCTTGTAT CCCACCACAC 
AGACTCAGTA AGATCACAAA CCGCTTGACC ATCCAGAGGA AGAGTCAGTT TATGCAAAGG 
TTACACAGCT ACTGGACCTT AAAGAGACAG TCTCGGAATG GTGTCCCGCT TCTACGTCGC 
CTGCAGACGC ACCTACAGTC TCAAAGGAAC TGCGACCAGG AGATGAAGTG GCGGATACCA 
ATTTCTGATG ACACGCCAGG TCACCTTGGT ACCATCGCTA TTTCTAACGA GGAGCCCGGA 
GACCAAGCTA ACAAGAATGA CCCTACGGAT AAGAACTGGG TGCTAAAGGA ACAATTAAAG 
TCATGGCAGA GACTTCGGCA CGACTTGGAG AGAGCTCGCC TTCTGGTAGA ATTAATACGA 
AAGCGCGAGA AGCTCAAGAG AGAGACGATT AAAGTCCAGC AAATGGCACT GGAAATGCAG 
CTGACTCCAT TTCTTATCCT ACTGAGAAAA ACCCTGGAGC TGCTGCAGGA GAAAGATACA 
AGTAATATAT TCACTGAGCC AGTCCCTCTG TCTGAGGTAA CAGAAATCTA CGAAGTGCCA 
GACTACCTAG AACATGTTAA GAAGCCAATG GACTTTCAGA CCATGAAGCG TAACTTAGAG 
GCTTTCCGTT ACCAGAATTT CGACCAGTTT GAGGAAGACT TCAACTTAAT TGTGAACAAT 
TGCATAAAGT ACAACGCTAA AGACACTATT TTTTACCGAG CCGCATCCCG CCTACGAGAA 
CAGGGAGCAG CCCTGCTGCG GCAGGCCCGC AAGCAGGCGG AAAAGATTGG CATTGATTTC 
GAGACGGGTA TGCATATACA CCCAGTCATC ACAGGAAATG AGATTGGGCG CCATGGCAGA 
GAGAATGACT TTGAGCGTGC TCATCTGTCT GACAGCCGCA AGCAACTCCC TTTAGAGGAG 
CAGCTGAAAG TCCAGTTGGC GAGACTGGAT GAGGTGAATG CAAGTAAGCA AAGCATAGGA 
CGAGCGCGCC GGGCCAAGAT GATCCGCAAG GAGATCACGT CGATCCGTAG GAAGATGGTT 
TACCAACAAG AATCCAGAAG GGACGGTGGC TCTGAGCGGC ACAGCTCAGC CGGGAGAGGA 
GCCCTGCCTT TGCACGGCCC TTCAGAGAAG GATGGACAGA CGGACAGTGC TGCAGAAGAG 
AGCAGCAGCC AAGAGACCGG GAAAGGACTT GGCCCAAACA CTTCCTCCAC CAATGCTCAT 
GAAGTTGGTA GAAGGACTTC TGTCCTTTTC TCTAAGAAGA ACCCCAAAAC TGCAGGACCA 
CCTAAGAGAC CAGGAAGGCC ACCTAAGAAT AGAGACAATC AGCTCTCATC GGGACAGATC 
AGCAGCCCCA TTGGTCCCCC ACAGCTGTCT GTCACAGGGG GATCTCAAAG GCGAAAGAGA 
GTCCGAAGCC CTCGGCCAAC TTCAAGCTCT GATAGCGACA GTGACAGTGA AAAGTCAGCA 
GAGGAACCCT CTTTAGATTT GCCTACAAAT GGCTTCAGCA GCAGCACCCA GCAAGTCAAA 
AAGAGTTTTT TGGTTTACCG GACCGACTGC AATCTTCCAC GAAGCAGCTC AGACTCTGAG 
TCCAGCACCA CCAGCAGCAG CAGTGCTGCT TCCGACAGGA CCAGCACAAC TCCTTCCAAG 
CAGGGTCGGG GGAAGCCATC TTTCTCTAGG GATAATTTTG CTGACTACAG CAGTGAGGAT 
ACATCTGGAA CCGAGAATGA GTCTTACTCC GTTGGTGGGA GAGTCGGGCA CGGCTTGGTA 
CGCAAAGGAC TGGAAAGCCG AGGGGCAGGG TGGCTGTCTG AAGATGAAGA TTCCCCTCTA 
GAAGCTTTAG ACTTGGTGTG GGCCAAGTGT CGGGGCTATC CCTCCTATCC AGCTCTGATC 
ATTGACCCAA AAATGCCACG AGAAGGAGTG TTTCACCATG GCGTTCCGAT TCCTGTGCCA 
CCCGTGGATG TTCTCAAATT AGGAGAGCAG ATGGCCCAAG AGGCACAAGA GCATCTCTAC 
CTCGTCCTGT TTTTTGACAA CAAGAGAACC TGGCAGTGGC TTCCTCGGAC AAAGTTAGTC 
CCTCTCGGCA TGAACCGGGA ATTGGATAAG GAGAAGATGC TGGAAGGAAG AAAATCAAAC 
ATCCGCAAAT CTGTGCAGAT CGCCTATGAC CGTGCCATGC AGCACCGTAA CAAAGTACAG 
GGCGATCCAA GCAGTGAATC CAGTGAGAGC GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brpf1 ( XM_018092898.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948387.1 Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa43710
Clone ID Related Accession (Same CDS sequence)	XM_018092898.1
Accession Version	XM_018092898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	peregrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG TTGACTTTGA TGTGAAGACA TTTTGCCACA ACTTGAGGGC CACCAAGCCT 
CCCTATGAAT GTCCGGTGGA GACCTGCCGA AAGATCTACA AGAGCTACAG TGGGATCGAG 
TACCACCTGT ATCACTACGA TCATGACAAC CCGCCTCTGC AGCAGCAGAC TCCTATCCGC 
AAATCAAAAA AGAAAGGCCG GCACCCCCGA TCCGCCAATA AGCAGTCACC GACGAACTCG 
GAAGTGTCTC AGTCTCCGAC CCGGGAGGTG ATGACTTACG CCCAGGCCCA GAGACTGGTG 
GAGGTGGAAT TGCACGGGCG CGTGCATCGT ATAAGTATAT TTGACAACTT GGATGTTGTT 
TCTGAGGATG ATGACCCGCC CGAAGAAACC CCAGAAAATG GAAGTAATAA GGAAAATAAT 
GACACCCCCG CGACCACTCC TAAGTCGGGA AAGCACAAAA ACAAGGATAA GAGAAAAGAC 
TCCAACCATC ATCATCACCA CAGTGCCACC GCAGGCGCCG CCACCAAACT CCCAGAGGTT 
GTGTACCGAG AGCTCGAACT GGACTCGCCA GATGCTCCTC CGCGGCCTTC TTCCTATTAC 
AGGTACATTG AGAAATCGGC AGAGGAACTA GACGAGGAGG TGGAGTATGA TATGGATGAG 
GAAGATTACA TCTGGCTTGA TATAATGAAT GATCGAAGGA AGAACGATGG AGTGAACCAT 
ATCCCACAAG AGATCTTTGA GTATCTAATG GACAGGTTGG AAAAGGAATC TTACTTTGAA 
AGCCATAATA AAGGGGACCC AAATTCTTTA ATTGACGAGG ATGCTGTATG CTGCATTTGC 
AACGATGGCG AGTGTCAGAA CAGCAATGTT ATATTGTTCT GCGATATGTG TAACCTGGCT 
GTGCACCAGG AGTGCTATGG GGTCCCTTAC ATACCAGAAG GACAATGGCT TTGTAGGAGG 
TGCCTCCAAT CACCTTCTAG GGCAGTGGAT TGTGCACTGT GCCCAAATAA GGGTGGGGCA 
TTCAAACAGA CAGATGATGG GCGCTGGGCA CACGTAGTTT GTGCCCTTTG GATTCCCGAG 
GTTTGTTTTG CAAACACAGT CTTCCTGGAA CCCATAGACA GCATTGAACA TATCCCACCT 
GCGCGTTGGA AACTGACATG CTATATCTGC AAACAGCGAG GCTCTGGAGC TTGCATCCAG 
TGCCACAAAG CAAACTGTTA TACAGCCTTC CATGTGACAT GCGCTCAGCA GGCTGGCCTG 
TACATGAAAA TGGAACCTGT GCGAGAGACT GGTGCTAACG GCACTTCTTT TAGTGTCCGC 
AAGACTGCGT ACTGTGATAT TCACACGCCT CCTGGGTCTG TTTGCAAGCT CCCAGCACTT 
TCTCATAGTG AAGGAGAGGA GGAGGATGAT GAGGAAGAAG AAGATGGCAA AGGATGGAGC 
TCTGAAAAAG TAAAAAAAGC AAAGGCCAAA TCAAGGATCA AAATGAAGAA AGCTCGGAAG 
ATCCTTGCTG AAAAGAGAGC TGCTGCCCCT GTTGTTTCTG TTCCTTGTAT CCCACCACAC 
AGACTCAGTA AGATCACAAA CCGCTTGACC ATCCAGAGGA AGAGTCAGTT TATGCAAAGG 
TTACACAGCT ACTGGACCTT AAAGAGACAG TCTCGGAATG GTGTCCCGCT TCTACGTCGC 
CTGCAGACGC ACCTACAGTC TCAAAGGAAC TGCGACCAGA AGGCAAGCTC CAGTGAGTCT 
GAGACGACCT ATTCAGCCTG CCTGTCTGCC TTTGGAACTG TACAGGAGAT GAAGTGGCGG 
ATACCAATTT CTGATGACAC GCCAGGTCAC CTTGGTACCA TCGCTATTTC TAACGAGGAG 
CCCGGAGACC AAGCTAACAA GAATGACCCT ACGGATAAGA ACTGGGTGCT AAAGGAACAA 
TTAAAGTCAT GGCAGAGACT TCGGCACGAC TTGGAGAGAG CTCGCCTTCT GGTAGAATTA 
ATACGAAAGC GCGAGAAGCT CAAGAGAGAG ACGATTAAAG TCCAGCAAAT GGCACTGGAA 
ATGCAGCTGA CTCCATTTCT TATCCTACTG AGAAAAACCC TGGAGCTGCT GCAGGAGAAA 
GATACAAGTA ATATATTCAC TGAGCCAGTC CCTCTGTCTG AGGTGCCAGA CTACCTAGAA 
CATGTTAAGA AGCCAATGGA CTTTCAGACC ATGAAGCGTA ACTTAGAGGC TTTCCGTTAC 
CAGAATTTCG ACCAGTTTGA GGAAGACTTC AACTTAATTG TGAACAATTG CATAAAGTAC 
AACGCTAAAG ACACTATTTT TTACCGAGCC GCATCCCGCC TACGAGAACA GGGAGCAGCC 
CTGCTGCGGC AGGCCCGCAA GCAGGCGGAA AAGATTGGCA TTGATTTCGA GACGGGTATG 
CATATACACC CAGTCATCAC AGGAAATGAG ATTGGGCGCC ATGGCAGAGA GAATGACTTT 
GAGCGTGCTC ATCTGTCTGA CAGCCGCAAG CAACTCCCTT TAGAGGAGCA GCTGAAAGTC 
CAGTTGGCGA GACTGGATGA GGTGAATGCA AGTAAGCAAA GCATAGGACG AGCGCGCCGG 
GCCAAGATGA TCCGCAAGGA GATCACGTCG ATCCGTAGGA AGATGGTTTA CCAACAAGAA 
TCCAGAAGGG ACGGTGGCTC TGAGCGGCAC AGCTCAGCCG GGAGAGGAGC CCTGCCTTTG 
CACGGCCCTT CAGAGAAGGA TGGACAGACG GACAGTGCTG CAGAAGAGAG CAGCAGCCAA 
GAGACCGGGA AAGGACTTGG CCCAAACACT TCCTCCACCA ATGCTCATGA AGTTGGTAGA 
AGGACTTCTG TCCTTTTCTC TAAGAAGAAC CCCAAAACTG CAGGACCACC TAAGAGACCA 
GGAAGGCCAC CTAAGAATAG AGACAATCAG CTCTCATCGG GACAGATCAG CAGCCCCATT 
GGTCCCCCAC AGCTGTCTGT CACAGGGGGA TCTCAAAGGC GAAAGAGAGT CCGAAGCCCT 
CGGCCAACTT CAAGCTCTGA TAGCGACAGT GACAGTGAAA AGTCAGCAGA GGAACCCTCT 
TTAGATTTGC CTACAAATGG CTTCAGCAGC AGCACCCAGC AAGTCAAAAA GAGTTTTTTG 
GTTTACCGGA CCGACTGCAA TCTTCCACGA AGCAGCTCAG ACTCTGAGTC CAGCACCACC 
AGCAGCAGCA GTGCTGCTTC CGACAGGACC AGCACAACTC CTTCCAAGCA GGGTCGGGGG 
AAGCCATCTT TCTCTAGGGA TAATTTTGCT GACTACAGCA GTGAGGATAC ATCTGGAACC 
GAGAATGAGT CTTACTCCGT TGGTGGGAGA GTCGGGCACG GCTTGGTACG CAAAGGACTG 
GAAAGCCGAG GGGCAGGGTG GCTGTCTGAA GATGAAGATT CCCCTCTAGA AGCTTTAGAC 
TTGGTGTGGG CCAAGTGTCG GGGCTATCCC TCCTATCCAG CTCTGATCAT TGACCCAAAA 
ATGCCACGAG AAGGAGTGTT TCACCATGGC GTTCCGATTC CTGTGCCACC CGTGGATGTT 
CTCAAATTAG GAGAGCAGAT GGCCCAAGAG GCACAAGAGC ATCTCTACCT CGTCCTGTTT 
TTTGACAACA AGAGAACCTG GCAGTGGCTT CCTCGGACAA AGTTAGTCCC TCTCGGCATG 
AACCGGGAAT TGGATAAGGA GAAGATGCTG GAAGGAAGAA AATCAAACAT CCGCAAATCT 
GTGCAGATCG CCTATGACCG TGCCATGCAG CACCGTAACA AAGTACAGGG CGATCCAAGC 
AGTGAATCCA GTGAGAGCGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948387.1
CDS	244..4047
Translation	MMGVDFDVKTFCHNLRATKPPYECPVETCRKIYKSYSGIEYHLYHYDHDNPPLQQQTPIRKSKKKGRHPRSANKQSPTNSEVSQSPTREVMTYAQAQRLVEVELHGRVHRISIFDNLDVVSEDDDPPEETPENGSNKENNDTPATTPKSGKHKNKDKRKDSNHHHHHSATAGAATKLPEVVYRELELDSPDAPPRPSSYYRYIEKSAEELDEEVEYDMDEEDYIWLDIMNDRRKNDGVNHIPQEIFEYLMDRLEKESYFESHNKGDPNSLIDEDAVCCICNDGECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCPNKGGAFKQTDDGRWAHVVCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLYMKMEPVRETGANGTSFSVRKTAYCDIHTPPGSVCKLPALSHSEGEEEDDEEEEDGKGWSSEKVKKAKAKSRIKMKKARKILAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQRNCDQKASSSESETTYSACLSAFGTVQEMKWRIPISDDTPGHLGTIAISNEEPGDQANKNDPTDKNWVLKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKVQQMALEMQLTPFLILLRKTLELLQEKDTSNIFTEPVPLSEVPDYLEHVKKPMDFQTMKRNLEAFRYQNFDQFEEDFNLIVNNCIKYNAKDTIFYRAASRLREQGAALLRQARKQAEKIGIDFETGMHIHPVITGNEIGRHGRENDFERAHLSDSRKQLPLEEQLKVQLARLDEVNASKQSIGRARRAKMIRKEITSIRRKMVYQQESRRDGGSERHSSAGRGALPLHGPSEKDGQTDSAAEESSSQETGKGLGPNTSSTNAHEVGRRTSVLFSKKNPKTAGPPKRPGRPPKNRDNQLSSGQISSPIGPPQLSVTGGSQRRKRVRSPRPTSSSDSDSDSEKSAEEPSLDLPTNGFSSSTQQVKKSFLVYRTDCNLPRSSSDSESSTTSSSSAASDRTSTTPSKQGRGKPSFSRDNFADYSSEDTSGTENESYSVGGRVGHGLVRKGLESRGAGWLSEDEDSPLEALDLVWAKCRGYPSYPALIIDPKMPREGVFHHGVPIPVPPVDVLKLGEQMAQEAQEHLYLVLFFDNKRTWQWLPRTKLVPLGMNRELDKEKMLEGRKSNIRKSVQIAYDRAMQHRNKVQGDPSSESSESD

Target ORF information:

RefSeq Version	XM_018092898.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092898.1

ORF Insert Sequence:

ATGATGGGTG TTGACTTTGA TGTGAAGACA TTTTGCCACA ACTTGAGGGC CACCAAGCCT 
CCCTATGAAT GTCCGGTGGA GACCTGCCGA AAGATCTACA AGAGCTACAG TGGGATCGAG 
TACCACCTGT ATCACTACGA TCATGACAAC CCGCCTCTGC AGCAGCAGAC TCCTATCCGC 
AAATCAAAAA AGAAAGGCCG GCACCCCCGA TCCGCCAATA AGCAGTCACC GACGAACTCG 
GAAGTGTCTC AGTCTCCGAC CCGGGAGGTG ATGACTTACG CCCAGGCCCA GAGACTGGTG 
GAGGTGGAAT TGCACGGGCG CGTGCATCGT ATAAGTATAT TTGACAACTT GGATGTTGTT 
TCTGAGGATG ATGACCCGCC CGAAGAAACC CCAGAAAATG GAAGTAATAA GGAAAATAAT 
GACACCCCCG CGACCACTCC TAAGTCGGGA AAGCACAAAA ACAAGGATAA GAGAAAAGAC 
TCCAACCATC ATCATCACCA CAGTGCCACC GCAGGCGCCG CCACCAAACT CCCAGAGGTT 
GTGTACCGAG AGCTCGAACT GGACTCGCCA GATGCTCCTC CGCGGCCTTC TTCCTATTAC 
AGGTACATTG AGAAATCGGC AGAGGAACTA GACGAGGAGG TGGAGTATGA TATGGATGAG 
GAAGATTACA TCTGGCTTGA TATAATGAAT GATCGAAGGA AGAACGATGG AGTGAACCAT 
ATCCCACAAG AGATCTTTGA GTATCTAATG GACAGGTTGG AAAAGGAATC TTACTTTGAA 
AGCCATAATA AAGGGGACCC AAATTCTTTA ATTGACGAGG ATGCTGTATG CTGCATTTGC 
AACGATGGCG AGTGTCAGAA CAGCAATGTT ATATTGTTCT GCGATATGTG TAACCTGGCT 
GTGCACCAGG AGTGCTATGG GGTCCCTTAC ATACCAGAAG GACAATGGCT TTGTAGGAGG 
TGCCTCCAAT CACCTTCTAG GGCAGTGGAT TGTGCACTGT GCCCAAATAA GGGTGGGGCA 
TTCAAACAGA CAGATGATGG GCGCTGGGCA CACGTAGTTT GTGCCCTTTG GATTCCCGAG 
GTTTGTTTTG CAAACACAGT CTTCCTGGAA CCCATAGACA GCATTGAACA TATCCCACCT 
GCGCGTTGGA AACTGACATG CTATATCTGC AAACAGCGAG GCTCTGGAGC TTGCATCCAG 
TGCCACAAAG CAAACTGTTA TACAGCCTTC CATGTGACAT GCGCTCAGCA GGCTGGCCTG 
TACATGAAAA TGGAACCTGT GCGAGAGACT GGTGCTAACG GCACTTCTTT TAGTGTCCGC 
AAGACTGCGT ACTGTGATAT TCACACGCCT CCTGGGTCTG TTTGCAAGCT CCCAGCACTT 
TCTCATAGTG AAGGAGAGGA GGAGGATGAT GAGGAAGAAG AAGATGGCAA AGGATGGAGC 
TCTGAAAAAG TAAAAAAAGC AAAGGCCAAA TCAAGGATCA AAATGAAGAA AGCTCGGAAG 
ATCCTTGCTG AAAAGAGAGC TGCTGCCCCT GTTGTTTCTG TTCCTTGTAT CCCACCACAC 
AGACTCAGTA AGATCACAAA CCGCTTGACC ATCCAGAGGA AGAGTCAGTT TATGCAAAGG 
TTACACAGCT ACTGGACCTT AAAGAGACAG TCTCGGAATG GTGTCCCGCT TCTACGTCGC 
CTGCAGACGC ACCTACAGTC TCAAAGGAAC TGCGACCAGA AGGCAAGCTC CAGTGAGTCT 
GAGACGACCT ATTCAGCCTG CCTGTCTGCC TTTGGAACTG TACAGGAGAT GAAGTGGCGG 
ATACCAATTT CTGATGACAC GCCAGGTCAC CTTGGTACCA TCGCTATTTC TAACGAGGAG 
CCCGGAGACC AAGCTAACAA GAATGACCCT ACGGATAAGA ACTGGGTGCT AAAGGAACAA 
TTAAAGTCAT GGCAGAGACT TCGGCACGAC TTGGAGAGAG CTCGCCTTCT GGTAGAATTA 
ATACGAAAGC GCGAGAAGCT CAAGAGAGAG ACGATTAAAG TCCAGCAAAT GGCACTGGAA 
ATGCAGCTGA CTCCATTTCT TATCCTACTG AGAAAAACCC TGGAGCTGCT GCAGGAGAAA 
GATACAAGTA ATATATTCAC TGAGCCAGTC CCTCTGTCTG AGGTGCCAGA CTACCTAGAA 
CATGTTAAGA AGCCAATGGA CTTTCAGACC ATGAAGCGTA ACTTAGAGGC TTTCCGTTAC 
CAGAATTTCG ACCAGTTTGA GGAAGACTTC AACTTAATTG TGAACAATTG CATAAAGTAC 
AACGCTAAAG ACACTATTTT TTACCGAGCC GCATCCCGCC TACGAGAACA GGGAGCAGCC 
CTGCTGCGGC AGGCCCGCAA GCAGGCGGAA AAGATTGGCA TTGATTTCGA GACGGGTATG 
CATATACACC CAGTCATCAC AGGAAATGAG ATTGGGCGCC ATGGCAGAGA GAATGACTTT 
GAGCGTGCTC ATCTGTCTGA CAGCCGCAAG CAACTCCCTT TAGAGGAGCA GCTGAAAGTC 
CAGTTGGCGA GACTGGATGA GGTGAATGCA AGTAAGCAAA GCATAGGACG AGCGCGCCGG 
GCCAAGATGA TCCGCAAGGA GATCACGTCG ATCCGTAGGA AGATGGTTTA CCAACAAGAA 
TCCAGAAGGG ACGGTGGCTC TGAGCGGCAC AGCTCAGCCG GGAGAGGAGC CCTGCCTTTG 
CACGGCCCTT CAGAGAAGGA TGGACAGACG GACAGTGCTG CAGAAGAGAG CAGCAGCCAA 
GAGACCGGGA AAGGACTTGG CCCAAACACT TCCTCCACCA ATGCTCATGA AGTTGGTAGA 
AGGACTTCTG TCCTTTTCTC TAAGAAGAAC CCCAAAACTG CAGGACCACC TAAGAGACCA 
GGAAGGCCAC CTAAGAATAG AGACAATCAG CTCTCATCGG GACAGATCAG CAGCCCCATT 
GGTCCCCCAC AGCTGTCTGT CACAGGGGGA TCTCAAAGGC GAAAGAGAGT CCGAAGCCCT 
CGGCCAACTT CAAGCTCTGA TAGCGACAGT GACAGTGAAA AGTCAGCAGA GGAACCCTCT 
TTAGATTTGC CTACAAATGG CTTCAGCAGC AGCACCCAGC AAGTCAAAAA GAGTTTTTTG 
GTTTACCGGA CCGACTGCAA TCTTCCACGA AGCAGCTCAG ACTCTGAGTC CAGCACCACC 
AGCAGCAGCA GTGCTGCTTC CGACAGGACC AGCACAACTC CTTCCAAGCA GGGTCGGGGG 
AAGCCATCTT TCTCTAGGGA TAATTTTGCT GACTACAGCA GTGAGGATAC ATCTGGAACC 
GAGAATGAGT CTTACTCCGT TGGTGGGAGA GTCGGGCACG GCTTGGTACG CAAAGGACTG 
GAAAGCCGAG GGGCAGGGTG GCTGTCTGAA GATGAAGATT CCCCTCTAGA AGCTTTAGAC 
TTGGTGTGGG CCAAGTGTCG GGGCTATCCC TCCTATCCAG CTCTGATCAT TGACCCAAAA 
ATGCCACGAG AAGGAGTGTT TCACCATGGC GTTCCGATTC CTGTGCCACC CGTGGATGTT 
CTCAAATTAG GAGAGCAGAT GGCCCAAGAG GCACAAGAGC ATCTCTACCT CGTCCTGTTT 
TTTGACAACA AGAGAACCTG GCAGTGGCTT CCTCGGACAA AGTTAGTCCC TCTCGGCATG 
AACCGGGAAT TGGATAAGGA GAAGATGCTG GAAGGAAGAA AATCAAACAT CCGCAAATCT 
GTGCAGATCG CCTATGACCG TGCCATGCAG CACCGTAACA AAGTACAGGG CGATCCAAGC 
AGTGAATCCA GTGAGAGCGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brpf1 ( XM_012960522.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815976.1 Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa30956
Clone ID Related Accession (Same CDS sequence)	XM_012960522.2 , XM_012960522.3
Accession Version	XM_012960522.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	peregrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960522.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG TTGACTTTGA TGTGAAGACA TTTTGCCACA ACTTGAGGGC CACCAAGCCT 
CCCTATGAAT GTCCGGTGGA GACCTGCCGA AAGATCTACA AGAGCTACAG TGGGATCGAG 
TACCACCTGT ATCACTACGA TCATGACAAC CCGCCTCTGC AGCAGCAGAC TCCTATCCGC 
AAATCAAAAA AGAAAGGCCG GCACCCCCGA TCCGCCAATA AGCAGTCACC GACGAACTCG 
GAAGTGTCTC AGTCTCCGAC CCGGGAGGTG ATGACTTACG CCCAGGCCCA GAGACTGGTG 
GAGGTGGAAT TGCACGGGCG CGTGCATCGT ATAAGTATAT TTGACAACTT GGATGTTGTT 
TCTGAGGATG ATGACCCGCC CGAAGAAACC CCAGAAAATG GAAGTAATAA GGAAAATAAT 
GACACCCCCG CGACCACTCC TAAGTCGGGA AAGCACAAAA ACAAGGATAA GAGAAAAGAC 
TCCAACCATC ATCATCACCA CAGTGCCACC GCAGGCGCCG CCACCAAACT CCCAGAGGTT 
GTGTACCGAG AGCTCGAACT GGACTCGCCA GATGCTCCTC CGCGGCCTTC TTCCTATTAC 
AGGTACATTG AGAAATCGGC AGAGGAACTA GACGAGGAGG TGGAGTATGA TATGGATGAG 
GAAGATTACA TCTGGCTTGA TATAATGAAT GATCGAAGGA AGAACGATGG AGTGAACCAT 
ATCCCACAAG AGATCTTTGA GTATCTAATG GACAGGTTGG AAAAGGAATC TTACTTTGAA 
AGCCATAATA AAGGGGACCC AAATTCTTTA ATTGACGAGG ATGCTGTATG CTGCATTTGC 
AACGATGGCG AGTGTCAGAA CAGCAATGTT ATATTGTTCT GCGATATGTG TAACCTGGCT 
GTGCACCAGG AGTGCTATGG GGTCCCTTAC ATACCAGAAG GACAATGGCT TTGTAGGAGG 
TGCCTCCAAT CACCTTCTAG GGCAGTGGAT TGTGCACTGT GCCCAAATAA GGGTGGGGCA 
TTCAAACAGA CAGATGATGG GCGCTGGGCA CACGTAGTTT GTGCCCTTTG GATTCCCGAG 
GTTTGTTTTG CAAACACAGT CTTCCTGGAA CCCATAGACA GCATTGAACA TATCCCACCT 
GCGCGTTGGA AACTGACATG CTATATCTGC AAACAGCGAG GCTCTGGAGC TTGCATCCAG 
TGCCACAAAG CAAACTGTTA TACAGCCTTC CATGTGACAT GCGCTCAGCA GGCTGGCCTG 
TACATGAAAA TGGAACCTGT GCGAGAGACT GGTGCTAACG GCACTTCTTT TAGTGTCCGC 
AAGACTGCGT ACTGTGATAT TCACACGCCT CCTGGGTCTG TTTGCAAGCT CCCAGCACTT 
TCTCATAGTG AAGGAGAGGA GGAGGATGAT GAGGAAGAAG AAGATGGCAA AGGATGGAGC 
TCTGAAAAAG TAAAAAAAGC AAAGGCCAAA TCAAGGATCA AAATGAAGAA AGCTCGGAAG 
ATCCTTGCTG AAAAGAGAGC TGCTGCCCCT GTTGTTTCTG TTCCTTGTAT CCCACCACAC 
AGACTCAGTA AGATCACAAA CCGCTTGACC ATCCAGAGGA AGAGTCAGTT TATGCAAAGG 
TTACACAGCT ACTGGACCTT AAAGAGACAG TCTCGGAATG GTGTCCCGCT TCTACGTCGC 
CTGCAGACGC ACCTACAGTC TCAAAGGAAC TGCGACCAGA AGGCAAGCTC CAGTGAGTCT 
GAGACGACCT ATTCAGCCTG CCTGTCTGCC TTTGGAACTG TACAGGAGAT GAAGTGGCGG 
ATACCAATTT CTGATGACAC GCCAGGTCAC CTTGGTACCA TCGCTATTTC TAACGAGGAG 
CCCGGAGACC AAGCTAACAA GAATGACCCT ACGGATAAGA ACTGGGTGCT AAAGGAACAA 
TTAAAGTCAT GGCAGAGACT TCGGCACGAC TTGGAGAGAG CTCGCCTTCT GGTAGAATTA 
ATACGAAAGC GCGAGAAGCT CAAGAGAGAG ACGATTAAAG TCCAGCAAAT GGCACTGGAA 
ATGCAGCTGA CTCCATTTCT TATCCTACTG AGAAAAACCC TGGAGCTGCT GCAGGAGAAA 
GATACAAGTA ATATATTCAC TGAGCCAGTC CCTCTGTCTG AGGTAACAGA AATCTACGAA 
GTGCCAGACT ACCTAGAACA TGTTAAGAAG CCAATGGACT TTCAGACCAT GAAGCGTAAC 
TTAGAGGCTT TCCGTTACCA GAATTTCGAC CAGTTTGAGG AAGACTTCAA CTTAATTGTG 
AACAATTGCA TAAAGTACAA CGCTAAAGAC ACTATTTTTT ACCGAGCCGC ATCCCGCCTA 
CGAGAACAGG GAGCAGCCCT GCTGCGGCAG GCCCGCAAGC AGGCGGAAAA GATTGGCATT 
GATTTCGAGA CGGGTATGCA TATACACCCA GTCATCACAG GAAATGAGAT TGGGCGCCAT 
GGCAGAGAGA ATGACTTTGA GCGTGCTCAT CTGTCTGACA GCCGCAAGCA ACTCCCTTTA 
GAGGAGCAGC TGAAAGTCCA GTTGGCGAGA CTGGATGAGG TGAATGCAAG TAAGCAAAGC 
ATAGGACGAG CGCGCCGGGC CAAGATGATC CGCAAGGAGA TCACGTCGAT CCGTAGGAAG 
ATGGTTTACC AACAAGAATC CAGAAGGGAC GGTGGCTCTG AGCGGCACAG CTCAGCCGGG 
AGAGGAGCCC TGCCTTTGCA CGGCCCTTCA GAGAAGGATG GACAGACGGA CAGTGCTGCA 
GAAGAGAGCA GCAGCCAAGA GACCGGGAAA GGACTTGGCC CAAACACTTC CTCCACCAAT 
GCTCATGAAG TTGGTAGAAG GACTTCTGTC CTTTTCTCTA AGAAGAACCC CAAAACTGCA 
GGACCACCTA AGAGACCAGG AAGGCCACCT AAGAATAGAG ACAATCAGCT CTCATCGGGA 
CAGATCAGCA GCCCCATTGG TCCCCCACAG CTGTCTGTCA CAGGGGGATC TCAAAGGCGA 
AAGAGAGTCC GAAGCCCTCG GCCAACTTCA AGCTCTGATA GCGACAGTGA CAGTGAAAAG 
TCAGCAGAGG AACCCTCTTT AGATTTGCCT ACAAATGGCT TCAGCAGCAG CACCCAGCAA 
GTCAAAAAGA GTTTTTTGGT TTACCGGACC GACTGCAATC TTCCACGAAG CAGCTCAGAC 
TCTGAGTCCA GCACCACCAG CAGCAGCAGT GCTGCTTCCG ACAGGACCAG CACAACTCCT 
TCCAAGCAGG GTCGGGGGAA GCCATCTTTC TCTAGGGATA ATTTTGCTGA CTACAGCAGT 
GAGGATACAT CTGGAACCGA GAATGAGTCT TACTCCGTTG GTGGGAGAGT CGGGCACGGC 
TTGGTACGCA AAGGACTGGA AAGCCGAGGG GCAGGGTGGC TGTCTGAAGA TGAAGATTCC 
CCTCTAGAAG CTTTAGACTT GGTGTGGGCC AAGTGTCGGG GCTATCCCTC CTATCCAGCT 
CTGATCATTG ACCCAAAAAT GCCACGAGAA GGAGTGTTTC ACCATGGCGT TCCGATTCCT 
GTGCCACCCG TGGATGTTCT CAAATTAGGA GAGCAGATGG CCCAAGAGGC ACAAGAGCAT 
CTCTACCTCG TCCTGTTTTT TGACAACAAG AGAACCTGGC AGTGGCTTCC TCGGACAAAG 
TTAGTCCCTC TCGGCATGAA CCGGGAATTG GATAAGGAGA AGATGCTGGA AGGAAGAAAA 
TCAAACATCC GCAAATCTGT GCAGATCGCC TATGACCGTG CCATGCAGCA CCGTAACAAA 
GTACAGGGCG ATCCAAGCAG TGAATCCAGT GAGAGCGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815976.1
CDS	244..4065
Translation	MMGVDFDVKTFCHNLRATKPPYECPVETCRKIYKSYSGIEYHLYHYDHDNPPLQQQTPIRKSKKKGRHPRSANKQSPTNSEVSQSPTREVMTYAQAQRLVEVELHGRVHRISIFDNLDVVSEDDDPPEETPENGSNKENNDTPATTPKSGKHKNKDKRKDSNHHHHHSATAGAATKLPEVVYRELELDSPDAPPRPSSYYRYIEKSAEELDEEVEYDMDEEDYIWLDIMNDRRKNDGVNHIPQEIFEYLMDRLEKESYFESHNKGDPNSLIDEDAVCCICNDGECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCPNKGGAFKQTDDGRWAHVVCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLYMKMEPVRETGANGTSFSVRKTAYCDIHTPPGSVCKLPALSHSEGEEEDDEEEEDGKGWSSEKVKKAKAKSRIKMKKARKILAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQRNCDQKASSSESETTYSACLSAFGTVQEMKWRIPISDDTPGHLGTIAISNEEPGDQANKNDPTDKNWVLKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKVQQMALEMQLTPFLILLRKTLELLQEKDTSNIFTEPVPLSEVTEIYEVPDYLEHVKKPMDFQTMKRNLEAFRYQNFDQFEEDFNLIVNNCIKYNAKDTIFYRAASRLREQGAALLRQARKQAEKIGIDFETGMHIHPVITGNEIGRHGRENDFERAHLSDSRKQLPLEEQLKVQLARLDEVNASKQSIGRARRAKMIRKEITSIRRKMVYQQESRRDGGSERHSSAGRGALPLHGPSEKDGQTDSAAEESSSQETGKGLGPNTSSTNAHEVGRRTSVLFSKKNPKTAGPPKRPGRPPKNRDNQLSSGQISSPIGPPQLSVTGGSQRRKRVRSPRPTSSSDSDSDSEKSAEEPSLDLPTNGFSSSTQQVKKSFLVYRTDCNLPRSSSDSESSTTSSSSAASDRTSTTPSKQGRGKPSFSRDNFADYSSEDTSGTENESYSVGGRVGHGLVRKGLESRGAGWLSEDEDSPLEALDLVWAKCRGYPSYPALIIDPKMPREGVFHHGVPIPVPPVDVLKLGEQMAQEAQEHLYLVLFFDNKRTWQWLPRTKLVPLGMNRELDKEKMLEGRKSNIRKSVQIAYDRAMQHRNKVQGDPSSESSESD

Target ORF information:

RefSeq Version	XM_012960522.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960522.2

ORF Insert Sequence:

ATGATGGGTG TTGACTTTGA TGTGAAGACA TTTTGCCACA ACTTGAGGGC CACCAAGCCT 
CCCTATGAAT GTCCGGTGGA GACCTGCCGA AAGATCTACA AGAGCTACAG TGGGATCGAG 
TACCACCTGT ATCACTACGA TCATGACAAC CCGCCTCTGC AGCAGCAGAC TCCTATCCGC 
AAATCAAAAA AGAAAGGCCG GCACCCCCGA TCCGCCAATA AGCAGTCACC GACGAACTCG 
GAAGTGTCTC AGTCTCCGAC CCGGGAGGTG ATGACTTACG CCCAGGCCCA GAGACTGGTG 
GAGGTGGAAT TGCACGGGCG CGTGCATCGT ATAAGTATAT TTGACAACTT GGATGTTGTT 
TCTGAGGATG ATGACCCGCC CGAAGAAACC CCAGAAAATG GAAGTAATAA GGAAAATAAT 
GACACCCCCG CGACCACTCC TAAGTCGGGA AAGCACAAAA ACAAGGATAA GAGAAAAGAC 
TCCAACCATC ATCATCACCA CAGTGCCACC GCAGGCGCCG CCACCAAACT CCCAGAGGTT 
GTGTACCGAG AGCTCGAACT GGACTCGCCA GATGCTCCTC CGCGGCCTTC TTCCTATTAC 
AGGTACATTG AGAAATCGGC AGAGGAACTA GACGAGGAGG TGGAGTATGA TATGGATGAG 
GAAGATTACA TCTGGCTTGA TATAATGAAT GATCGAAGGA AGAACGATGG AGTGAACCAT 
ATCCCACAAG AGATCTTTGA GTATCTAATG GACAGGTTGG AAAAGGAATC TTACTTTGAA 
AGCCATAATA AAGGGGACCC AAATTCTTTA ATTGACGAGG ATGCTGTATG CTGCATTTGC 
AACGATGGCG AGTGTCAGAA CAGCAATGTT ATATTGTTCT GCGATATGTG TAACCTGGCT 
GTGCACCAGG AGTGCTATGG GGTCCCTTAC ATACCAGAAG GACAATGGCT TTGTAGGAGG 
TGCCTCCAAT CACCTTCTAG GGCAGTGGAT TGTGCACTGT GCCCAAATAA GGGTGGGGCA 
TTCAAACAGA CAGATGATGG GCGCTGGGCA CACGTAGTTT GTGCCCTTTG GATTCCCGAG 
GTTTGTTTTG CAAACACAGT CTTCCTGGAA CCCATAGACA GCATTGAACA TATCCCACCT 
GCGCGTTGGA AACTGACATG CTATATCTGC AAACAGCGAG GCTCTGGAGC TTGCATCCAG 
TGCCACAAAG CAAACTGTTA TACAGCCTTC CATGTGACAT GCGCTCAGCA GGCTGGCCTG 
TACATGAAAA TGGAACCTGT GCGAGAGACT GGTGCTAACG GCACTTCTTT TAGTGTCCGC 
AAGACTGCGT ACTGTGATAT TCACACGCCT CCTGGGTCTG TTTGCAAGCT CCCAGCACTT 
TCTCATAGTG AAGGAGAGGA GGAGGATGAT GAGGAAGAAG AAGATGGCAA AGGATGGAGC 
TCTGAAAAAG TAAAAAAAGC AAAGGCCAAA TCAAGGATCA AAATGAAGAA AGCTCGGAAG 
ATCCTTGCTG AAAAGAGAGC TGCTGCCCCT GTTGTTTCTG TTCCTTGTAT CCCACCACAC 
AGACTCAGTA AGATCACAAA CCGCTTGACC ATCCAGAGGA AGAGTCAGTT TATGCAAAGG 
TTACACAGCT ACTGGACCTT AAAGAGACAG TCTCGGAATG GTGTCCCGCT TCTACGTCGC 
CTGCAGACGC ACCTACAGTC TCAAAGGAAC TGCGACCAGA AGGCAAGCTC CAGTGAGTCT 
GAGACGACCT ATTCAGCCTG CCTGTCTGCC TTTGGAACTG TACAGGAGAT GAAGTGGCGG 
ATACCAATTT CTGATGACAC GCCAGGTCAC CTTGGTACCA TCGCTATTTC TAACGAGGAG 
CCCGGAGACC AAGCTAACAA GAATGACCCT ACGGATAAGA ACTGGGTGCT AAAGGAACAA 
TTAAAGTCAT GGCAGAGACT TCGGCACGAC TTGGAGAGAG CTCGCCTTCT GGTAGAATTA 
ATACGAAAGC GCGAGAAGCT CAAGAGAGAG ACGATTAAAG TCCAGCAAAT GGCACTGGAA 
ATGCAGCTGA CTCCATTTCT TATCCTACTG AGAAAAACCC TGGAGCTGCT GCAGGAGAAA 
GATACAAGTA ATATATTCAC TGAGCCAGTC CCTCTGTCTG AGGTAACAGA AATCTACGAA 
GTGCCAGACT ACCTAGAACA TGTTAAGAAG CCAATGGACT TTCAGACCAT GAAGCGTAAC 
TTAGAGGCTT TCCGTTACCA GAATTTCGAC CAGTTTGAGG AAGACTTCAA CTTAATTGTG 
AACAATTGCA TAAAGTACAA CGCTAAAGAC ACTATTTTTT ACCGAGCCGC ATCCCGCCTA 
CGAGAACAGG GAGCAGCCCT GCTGCGGCAG GCCCGCAAGC AGGCGGAAAA GATTGGCATT 
GATTTCGAGA CGGGTATGCA TATACACCCA GTCATCACAG GAAATGAGAT TGGGCGCCAT 
GGCAGAGAGA ATGACTTTGA GCGTGCTCAT CTGTCTGACA GCCGCAAGCA ACTCCCTTTA 
GAGGAGCAGC TGAAAGTCCA GTTGGCGAGA CTGGATGAGG TGAATGCAAG TAAGCAAAGC 
ATAGGACGAG CGCGCCGGGC CAAGATGATC CGCAAGGAGA TCACGTCGAT CCGTAGGAAG 
ATGGTTTACC AACAAGAATC CAGAAGGGAC GGTGGCTCTG AGCGGCACAG CTCAGCCGGG 
AGAGGAGCCC TGCCTTTGCA CGGCCCTTCA GAGAAGGATG GACAGACGGA CAGTGCTGCA 
GAAGAGAGCA GCAGCCAAGA GACCGGGAAA GGACTTGGCC CAAACACTTC CTCCACCAAT 
GCTCATGAAG TTGGTAGAAG GACTTCTGTC CTTTTCTCTA AGAAGAACCC CAAAACTGCA 
GGACCACCTA AGAGACCAGG AAGGCCACCT AAGAATAGAG ACAATCAGCT CTCATCGGGA 
CAGATCAGCA GCCCCATTGG TCCCCCACAG CTGTCTGTCA CAGGGGGATC TCAAAGGCGA 
AAGAGAGTCC GAAGCCCTCG GCCAACTTCA AGCTCTGATA GCGACAGTGA CAGTGAAAAG 
TCAGCAGAGG AACCCTCTTT AGATTTGCCT ACAAATGGCT TCAGCAGCAG CACCCAGCAA 
GTCAAAAAGA GTTTTTTGGT TTACCGGACC GACTGCAATC TTCCACGAAG CAGCTCAGAC 
TCTGAGTCCA GCACCACCAG CAGCAGCAGT GCTGCTTCCG ACAGGACCAG CACAACTCCT 
TCCAAGCAGG GTCGGGGGAA GCCATCTTTC TCTAGGGATA ATTTTGCTGA CTACAGCAGT 
GAGGATACAT CTGGAACCGA GAATGAGTCT TACTCCGTTG GTGGGAGAGT CGGGCACGGC 
TTGGTACGCA AAGGACTGGA AAGCCGAGGG GCAGGGTGGC TGTCTGAAGA TGAAGATTCC 
CCTCTAGAAG CTTTAGACTT GGTGTGGGCC AAGTGTCGGG GCTATCCCTC CTATCCAGCT 
CTGATCATTG ACCCAAAAAT GCCACGAGAA GGAGTGTTTC ACCATGGCGT TCCGATTCCT 
GTGCCACCCG TGGATGTTCT CAAATTAGGA GAGCAGATGG CCCAAGAGGC ACAAGAGCAT 
CTCTACCTCG TCCTGTTTTT TGACAACAAG AGAACCTGGC AGTGGCTTCC TCGGACAAAG 
TTAGTCCCTC TCGGCATGAA CCGGGAATTG GATAAGGAGA AGATGCTGGA AGGAAGAAAA 
TCAAACATCC GCAAATCTGT GCAGATCGCC TATGACCGTG CCATGCAGCA CCGTAACAAA 
GTACAGGGCG ATCCAAGCAG TGAATCCAGT GAGAGCGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brpf1 ( XM_012960523.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815977.1 Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X3, mRNA.

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CloneID	OXa30957
Clone ID Related Accession (Same CDS sequence)	XM_012960523.3 , XM_012960523.2
Accession Version	XM_012960523.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	peregrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG TTGACTTTGA TGTGAAGACA TTTTGCCACA ACTTGAGGGC CACCAAGCCT 
CCCTATGAAT GTCCGGTGGA GACCTGCCGA AAGATCTACA AGAGCTACAG TGGGATCGAG 
TACCACCTGT ATCACTACGA TCATGACAAC CCGCCTCTGC AGCAGCAGAC TCCTATCCGC 
AAATCAAAAA AGAAAGGCCG GCACCCCCGA TCCGCCAATA AGCAGTCACC GACGAACTCG 
GAAGTGTCTC AGTCTCCGAC CCGGGAGGTG ATGACTTACG CCCAGGCCCA GAGACTGGTG 
GAGGTGGAAT TGCACGGGCG CGTGCATCGT ATAAGTATAT TTGACAACTT GGATGTTGTT 
TCTGAGGATG ATGACCCGCC CGAAGAAACC CCAGAAAATG GAAGTAATAA GGAAAATAAT 
GACACCCCCG CGACCACTCC TAAGTCGGGA AAGCACAAAA ACAAGGATAA GAGAAAAGAC 
TCCAACCATC ATCATCACCA CAGTGCCACC GCAGGCGCCG CCACCAAACT CCCAGAGGTT 
GTGTACCGAG AGCTCGAACT GGACTCGCCA GATGCTCCTC CGCGGCCTTC TTCCTATTAC 
AGGTACATTG AGAAATCGGC AGAGGAACTA GACGAGGAGG TGGAGTATGA TATGGATGAG 
GAAGATTACA TCTGGCTTGA TATAATGAAT GATCGAAGGA AGAACGATGG AGTGAACCAT 
ATCCCACAAG AGATCTTTGA GTATCTAATG GACAGGTTGG AAAAGGAATC TTACTTTGAA 
AGCCATAATA AAGGGGACCC AAATTCTTTA ATTGACGAGG ATGCTGTATG CTGCATTTGC 
AACGATGGCG AGTGTCAGAA CAGCAATGTT ATATTGTTCT GCGATATGTG TAACCTGGCT 
GTGCACCAGG AGTGCTATGG GGTCCCTTAC ATACCAGAAG GACAATGGCT TTGTAGGAGG 
TGCCTCCAAT CACCTTCTAG GGCAGTGGAT TGTGCACTGT GCCCAAATAA GGGTGGGGCA 
TTCAAACAGA CAGATGATGG GCGCTGGGCA CACGTAGTTT GTGCCCTTTG GATTCCCGAG 
GTTTGTTTTG CAAACACAGT CTTCCTGGAA CCCATAGACA GCATTGAACA TATCCCACCT 
GCGCGTTGGA AACTGACATG CTATATCTGC AAACAGCGAG GCTCTGGAGC TTGCATCCAG 
TGCCACAAAG CAAACTGTTA TACAGCCTTC CATGTGACAT GCGCTCAGCA GGCTGGCCTG 
TACATGAAAA TGGAACCTGT GCGAGAGACT GGTGCTAACG GCACTTCTTT TAGTGTCCGC 
AAGACTGCGT ACTGTGATAT TCACACGCCT CCTGGGTCTG TTTGCAAGCT CCCAGCACTT 
TCTCATAGTG AAGGAGAGGA GGAGGATGAT GAGGAAGAAG AAGATGGCAA AGGATGGAGC 
TCTGAAAAAG TAAAAAAAGC AAAGGCCAAA TCAAGGATCA AAATGAAGAA AGCTCGGAAG 
ATCCTTGCTG AAAAGAGAGC TGCTGCCCCT GTTGTTTCTG TTCCTTGTAT CCCACCACAC 
AGACTCAGTA AGATCACAAA CCGCTTGACC ATCCAGAGGA AGAGTCAGTT TATGCAAAGG 
TTACACAGCT ACTGGACCTT AAAGAGACAG TCTCGGAATG GTGTCCCGCT TCTACGTCGC 
CTGCAGACGC ACCTACAGTC TCAAAGGAAC TGCGACCAGG AGATGAAGTG GCGGATACCA 
ATTTCTGATG ACACGCCAGG TCACCTTGGT ACCATCGCTA TTTCTAACGA GGAGCCCGGA 
GACCAAGCTA ACAAGAATGA CCCTACGGAT AAGAACTGGG TGCTAAAGGA ACAATTAAAG 
TCATGGCAGA GACTTCGGCA CGACTTGGAG AGAGCTCGCC TTCTGGTAGA ATTAATACGA 
AAGCGCGAGA AGCTCAAGAG AGAGACGATT AAAGTCCAGC AAATGGCACT GGAAATGCAG 
CTGACTCCAT TTCTTATCCT ACTGAGAAAA ACCCTGGAGC TGCTGCAGGA GAAAGATACA 
AGTAATATAT TCACTGAGCC AGTCCCTCTG TCTGAGGTAA CAGAAATCTA CGAAGTGCCA 
GACTACCTAG AACATGTTAA GAAGCCAATG GACTTTCAGA CCATGAAGCG TAACTTAGAG 
GCTTTCCGTT ACCAGAATTT CGACCAGTTT GAGGAAGACT TCAACTTAAT TGTGAACAAT 
TGCATAAAGT ACAACGCTAA AGACACTATT TTTTACCGAG CCGCATCCCG CCTACGAGAA 
CAGGGAGCAG CCCTGCTGCG GCAGGCCCGC AAGCAGGCGG AAAAGATTGG CATTGATTTC 
GAGACGGGTA TGCATATACA CCCAGTCATC ACAGGAAATG AGATTGGGCG CCATGGCAGA 
GAGAATGACT TTGAGCGTGC TCATCTGTCT GACAGCCGCA AGCAACTCCC TTTAGAGGAG 
CAGCTGAAAG TCCAGTTGGC GAGACTGGAT GAGGTGAATG CAAGTAAGCA AAGCATAGGA 
CGAGCGCGCC GGGCCAAGAT GATCCGCAAG GAGATCACGT CGATCCGTAG GAAGATGGTT 
TACCAACAAG AATCCAGAAG GGACGGTGGC TCTGAGCGGC ACAGCTCAGC CGGGAGAGGA 
GCCCTGCCTT TGCACGGCCC TTCAGAGAAG GATGGACAGA CGGACAGTGC TGCAGAAGAG 
AGCAGCAGCC AAGAGACCGG GAAAGGACTT GGCCCAAACA CTTCCTCCAC CAATGCTCAT 
GAAGTTGGTA GAAGGACTTC TGTCCTTTTC TCTAAGAAGA ACCCCAAAAC TGCAGGACCA 
CCTAAGAGAC CAGGAAGGCC ACCTAAGAAT AGAGACAATC AGCTCTCATC GGGACAGATC 
AGCAGCCCCA TTGGTCCCCC ACAGCTGTCT GTCACAGGGG GATCTCAAAG GCGAAAGAGA 
GTCCGAAGCC CTCGGCCAAC TTCAAGCTCT GATAGCGACA GTGACAGTGA AAAGTCAGCA 
GAGGAACCCT CTTTAGATTT GCCTACAAAT GGCTTCAGCA GCAGCACCCA GCAAGTCAAA 
AAGAGTTTTT TGGTTTACCG GACCGACTGC AATCTTCCAC GAAGCAGCTC AGACTCTGAG 
TCCAGCACCA CCAGCAGCAG CAGTGCTGCT TCCGACAGGA CCAGCACAAC TCCTTCCAAG 
CAGGGTCGGG GGAAGCCATC TTTCTCTAGG GATAATTTTG CTGACTACAG CAGTGAGGAT 
ACATCTGGAA CCGAGAATGA GTCTTACTCC GTTGGTGGGA GAGTCGGGCA CGGCTTGGTA 
CGCAAAGGAC TGGAAAGCCG AGGGGCAGGG TGGCTGTCTG AAGATGAAGA TTCCCCTCTA 
GAAGCTTTAG ACTTGGTGTG GGCCAAGTGT CGGGGCTATC CCTCCTATCC AGCTCTGATC 
ATTGACCCAA AAATGCCACG AGAAGGAGTG TTTCACCATG GCGTTCCGAT TCCTGTGCCA 
CCCGTGGATG TTCTCAAATT AGGAGAGCAG ATGGCCCAAG AGGCACAAGA GCATCTCTAC 
CTCGTCCTGT TTTTTGACAA CAAGAGAACC TGGCAGTGGC TTCCTCGGAC AAAGTTAGTC 
CCTCTCGGCA TGAACCGGGA ATTGGATAAG GAGAAGATGC TGGAAGGAAG AAAATCAAAC 
ATCCGCAAAT CTGTGCAGAT CGCCTATGAC CGTGCCATGC AGCACCGTAA CAAAGTACAG 
GGCGATCCAA GCAGTGAATC CAGTGAGAGC GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815977.1
CDS	243..3998
Translation	MMGVDFDVKTFCHNLRATKPPYECPVETCRKIYKSYSGIEYHLYHYDHDNPPLQQQTPIRKSKKKGRHPRSANKQSPTNSEVSQSPTREVMTYAQAQRLVEVELHGRVHRISIFDNLDVVSEDDDPPEETPENGSNKENNDTPATTPKSGKHKNKDKRKDSNHHHHHSATAGAATKLPEVVYRELELDSPDAPPRPSSYYRYIEKSAEELDEEVEYDMDEEDYIWLDIMNDRRKNDGVNHIPQEIFEYLMDRLEKESYFESHNKGDPNSLIDEDAVCCICNDGECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCPNKGGAFKQTDDGRWAHVVCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLYMKMEPVRETGANGTSFSVRKTAYCDIHTPPGSVCKLPALSHSEGEEEDDEEEEDGKGWSSEKVKKAKAKSRIKMKKARKILAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQRNCDQEMKWRIPISDDTPGHLGTIAISNEEPGDQANKNDPTDKNWVLKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKVQQMALEMQLTPFLILLRKTLELLQEKDTSNIFTEPVPLSEVTEIYEVPDYLEHVKKPMDFQTMKRNLEAFRYQNFDQFEEDFNLIVNNCIKYNAKDTIFYRAASRLREQGAALLRQARKQAEKIGIDFETGMHIHPVITGNEIGRHGRENDFERAHLSDSRKQLPLEEQLKVQLARLDEVNASKQSIGRARRAKMIRKEITSIRRKMVYQQESRRDGGSERHSSAGRGALPLHGPSEKDGQTDSAAEESSSQETGKGLGPNTSSTNAHEVGRRTSVLFSKKNPKTAGPPKRPGRPPKNRDNQLSSGQISSPIGPPQLSVTGGSQRRKRVRSPRPTSSSDSDSDSEKSAEEPSLDLPTNGFSSSTQQVKKSFLVYRTDCNLPRSSSDSESSTTSSSSAASDRTSTTPSKQGRGKPSFSRDNFADYSSEDTSGTENESYSVGGRVGHGLVRKGLESRGAGWLSEDEDSPLEALDLVWAKCRGYPSYPALIIDPKMPREGVFHHGVPIPVPPVDVLKLGEQMAQEAQEHLYLVLFFDNKRTWQWLPRTKLVPLGMNRELDKEKMLEGRKSNIRKSVQIAYDRAMQHRNKVQGDPSSESSESD

Target ORF information:

RefSeq Version	XM_012960523.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and PHD finger containing 1 (brpf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960523.2

ORF Insert Sequence:

ATGATGGGTG TTGACTTTGA TGTGAAGACA TTTTGCCACA ACTTGAGGGC CACCAAGCCT 
CCCTATGAAT GTCCGGTGGA GACCTGCCGA AAGATCTACA AGAGCTACAG TGGGATCGAG 
TACCACCTGT ATCACTACGA TCATGACAAC CCGCCTCTGC AGCAGCAGAC TCCTATCCGC 
AAATCAAAAA AGAAAGGCCG GCACCCCCGA TCCGCCAATA AGCAGTCACC GACGAACTCG 
GAAGTGTCTC AGTCTCCGAC CCGGGAGGTG ATGACTTACG CCCAGGCCCA GAGACTGGTG 
GAGGTGGAAT TGCACGGGCG CGTGCATCGT ATAAGTATAT TTGACAACTT GGATGTTGTT 
TCTGAGGATG ATGACCCGCC CGAAGAAACC CCAGAAAATG GAAGTAATAA GGAAAATAAT 
GACACCCCCG CGACCACTCC TAAGTCGGGA AAGCACAAAA ACAAGGATAA GAGAAAAGAC 
TCCAACCATC ATCATCACCA CAGTGCCACC GCAGGCGCCG CCACCAAACT CCCAGAGGTT 
GTGTACCGAG AGCTCGAACT GGACTCGCCA GATGCTCCTC CGCGGCCTTC TTCCTATTAC 
AGGTACATTG AGAAATCGGC AGAGGAACTA GACGAGGAGG TGGAGTATGA TATGGATGAG 
GAAGATTACA TCTGGCTTGA TATAATGAAT GATCGAAGGA AGAACGATGG AGTGAACCAT 
ATCCCACAAG AGATCTTTGA GTATCTAATG GACAGGTTGG AAAAGGAATC TTACTTTGAA 
AGCCATAATA AAGGGGACCC AAATTCTTTA ATTGACGAGG ATGCTGTATG CTGCATTTGC 
AACGATGGCG AGTGTCAGAA CAGCAATGTT ATATTGTTCT GCGATATGTG TAACCTGGCT 
GTGCACCAGG AGTGCTATGG GGTCCCTTAC ATACCAGAAG GACAATGGCT TTGTAGGAGG 
TGCCTCCAAT CACCTTCTAG GGCAGTGGAT TGTGCACTGT GCCCAAATAA GGGTGGGGCA 
TTCAAACAGA CAGATGATGG GCGCTGGGCA CACGTAGTTT GTGCCCTTTG GATTCCCGAG 
GTTTGTTTTG CAAACACAGT CTTCCTGGAA CCCATAGACA GCATTGAACA TATCCCACCT 
GCGCGTTGGA AACTGACATG CTATATCTGC AAACAGCGAG GCTCTGGAGC TTGCATCCAG 
TGCCACAAAG CAAACTGTTA TACAGCCTTC CATGTGACAT GCGCTCAGCA GGCTGGCCTG 
TACATGAAAA TGGAACCTGT GCGAGAGACT GGTGCTAACG GCACTTCTTT TAGTGTCCGC 
AAGACTGCGT ACTGTGATAT TCACACGCCT CCTGGGTCTG TTTGCAAGCT CCCAGCACTT 
TCTCATAGTG AAGGAGAGGA GGAGGATGAT GAGGAAGAAG AAGATGGCAA AGGATGGAGC 
TCTGAAAAAG TAAAAAAAGC AAAGGCCAAA TCAAGGATCA AAATGAAGAA AGCTCGGAAG 
ATCCTTGCTG AAAAGAGAGC TGCTGCCCCT GTTGTTTCTG TTCCTTGTAT CCCACCACAC 
AGACTCAGTA AGATCACAAA CCGCTTGACC ATCCAGAGGA AGAGTCAGTT TATGCAAAGG 
TTACACAGCT ACTGGACCTT AAAGAGACAG TCTCGGAATG GTGTCCCGCT TCTACGTCGC 
CTGCAGACGC ACCTACAGTC TCAAAGGAAC TGCGACCAGG AGATGAAGTG GCGGATACCA 
ATTTCTGATG ACACGCCAGG TCACCTTGGT ACCATCGCTA TTTCTAACGA GGAGCCCGGA 
GACCAAGCTA ACAAGAATGA CCCTACGGAT AAGAACTGGG TGCTAAAGGA ACAATTAAAG 
TCATGGCAGA GACTTCGGCA CGACTTGGAG AGAGCTCGCC TTCTGGTAGA ATTAATACGA 
AAGCGCGAGA AGCTCAAGAG AGAGACGATT AAAGTCCAGC AAATGGCACT GGAAATGCAG 
CTGACTCCAT TTCTTATCCT ACTGAGAAAA ACCCTGGAGC TGCTGCAGGA GAAAGATACA 
AGTAATATAT TCACTGAGCC AGTCCCTCTG TCTGAGGTAA CAGAAATCTA CGAAGTGCCA 
GACTACCTAG AACATGTTAA GAAGCCAATG GACTTTCAGA CCATGAAGCG TAACTTAGAG 
GCTTTCCGTT ACCAGAATTT CGACCAGTTT GAGGAAGACT TCAACTTAAT TGTGAACAAT 
TGCATAAAGT ACAACGCTAA AGACACTATT TTTTACCGAG CCGCATCCCG CCTACGAGAA 
CAGGGAGCAG CCCTGCTGCG GCAGGCCCGC AAGCAGGCGG AAAAGATTGG CATTGATTTC 
GAGACGGGTA TGCATATACA CCCAGTCATC ACAGGAAATG AGATTGGGCG CCATGGCAGA 
GAGAATGACT TTGAGCGTGC TCATCTGTCT GACAGCCGCA AGCAACTCCC TTTAGAGGAG 
CAGCTGAAAG TCCAGTTGGC GAGACTGGAT GAGGTGAATG CAAGTAAGCA AAGCATAGGA 
CGAGCGCGCC GGGCCAAGAT GATCCGCAAG GAGATCACGT CGATCCGTAG GAAGATGGTT 
TACCAACAAG AATCCAGAAG GGACGGTGGC TCTGAGCGGC ACAGCTCAGC CGGGAGAGGA 
GCCCTGCCTT TGCACGGCCC TTCAGAGAAG GATGGACAGA CGGACAGTGC TGCAGAAGAG 
AGCAGCAGCC AAGAGACCGG GAAAGGACTT GGCCCAAACA CTTCCTCCAC CAATGCTCAT 
GAAGTTGGTA GAAGGACTTC TGTCCTTTTC TCTAAGAAGA ACCCCAAAAC TGCAGGACCA 
CCTAAGAGAC CAGGAAGGCC ACCTAAGAAT AGAGACAATC AGCTCTCATC GGGACAGATC 
AGCAGCCCCA TTGGTCCCCC ACAGCTGTCT GTCACAGGGG GATCTCAAAG GCGAAAGAGA 
GTCCGAAGCC CTCGGCCAAC TTCAAGCTCT GATAGCGACA GTGACAGTGA AAAGTCAGCA 
GAGGAACCCT CTTTAGATTT GCCTACAAAT GGCTTCAGCA GCAGCACCCA GCAAGTCAAA 
AAGAGTTTTT TGGTTTACCG GACCGACTGC AATCTTCCAC GAAGCAGCTC AGACTCTGAG 
TCCAGCACCA CCAGCAGCAG CAGTGCTGCT TCCGACAGGA CCAGCACAAC TCCTTCCAAG 
CAGGGTCGGG GGAAGCCATC TTTCTCTAGG GATAATTTTG CTGACTACAG CAGTGAGGAT 
ACATCTGGAA CCGAGAATGA GTCTTACTCC GTTGGTGGGA GAGTCGGGCA CGGCTTGGTA 
CGCAAAGGAC TGGAAAGCCG AGGGGCAGGG TGGCTGTCTG AAGATGAAGA TTCCCCTCTA 
GAAGCTTTAG ACTTGGTGTG GGCCAAGTGT CGGGGCTATC CCTCCTATCC AGCTCTGATC 
ATTGACCCAA AAATGCCACG AGAAGGAGTG TTTCACCATG GCGTTCCGAT TCCTGTGCCA 
CCCGTGGATG TTCTCAAATT AGGAGAGCAG ATGGCCCAAG AGGCACAAGA GCATCTCTAC 
CTCGTCCTGT TTTTTGACAA CAAGAGAACC TGGCAGTGGC TTCCTCGGAC AAAGTTAGTC 
CCTCTCGGCA TGAACCGGGA ATTGGATAAG GAGAAGATGC TGGAAGGAAG AAAATCAAAC 
ATCCGCAAAT CTGTGCAGAT CGCCTATGAC CGTGCCATGC AGCACCGTAA CAAAGTACAG 
GGCGATCCAA GCAGTGAATC CAGTGAGAGC GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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brpf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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