kif1a gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_018094451.1	XP_017949940.1	kinesin-like protein KIF1A isoform X1
XM_031902552.1	XP_031758412.1	kinesin-like protein KIF1A isoform X2
XM_031902554.1	XP_031758414.1	kinesin-like protein KIF1A isoform X4
XM_012971269.3	XP_012826723.1	kinesin-like protein KIF1A isoform X12
XM_012971268.2	XP_012826722.1	kinesin-like protein KIF1A isoform X5
XM_012971269.2	XP_012826723.1	kinesin-like protein KIF1A isoform X6
XM_012971268.3	XP_012826722.1	kinesin-like protein KIF1A isoform X11
XM_018094455.2	XP_017949944.1	kinesin-like protein KIF1A isoform X13
XM_018094454.1	XP_017949943.1	kinesin-like protein KIF1A isoform X4
XM_018094456.1	XP_017949945.1	kinesin-like protein KIF1A isoform X8
XM_031902555.1	XP_031758415.1	kinesin-like protein KIF1A isoform X5
XM_031902559.1	XP_031758419.1	kinesin-like protein KIF1A isoform X9
XM_018094452.1	XP_017949941.1	kinesin-like protein KIF1A isoform X2
XM_018094455.1	XP_017949944.1	kinesin-like protein KIF1A isoform X7
XM_031902551.1	XP_031758411.1	kinesin-like protein KIF1A isoform X1
XM_031902557.1	XP_031758417.1	kinesin-like protein KIF1A isoform X7
XM_031902558.1	XP_031758418.1	kinesin-like protein KIF1A isoform X8
XM_018094453.1	XP_017949942.1	kinesin-like protein KIF1A isoform X3
XM_031902553.1	XP_031758413.1	kinesin-like protein KIF1A isoform X3
XM_031902556.1	XP_031758416.1	kinesin-like protein KIF1A isoform X6
XM_031902560.1	XP_031758420.1	kinesin-like protein KIF1A isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa30861	XM_012971268.3 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa30862	XM_012971269.3 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa44798	XM_018094451.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa44799	XM_018094452.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa44800	XM_018094453.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa44801	XM_018094454.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa44802	XM_018094455.2 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa44803	XM_018094456.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa58871	XM_031902551.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58872	XM_031902552.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58873	XM_031902553.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58874	XM_031902554.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58875	XM_031902555.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58876	XM_031902556.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58877	XM_031902557.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58878	XM_031902558.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58879	XM_031902559.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58880	XM_031902560.1 Latest version!	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OXa30862	XM_012971269.2	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa30861	XM_012971268.2	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa44802	XM_018094455.1	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

kif1a ( XM_012971268.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826722.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OXa30861
Clone ID Related Accession (Same CDS sequence)	XM_012971268.2 , XM_012971268.3
Accession Version	XM_012971268.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971268.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
TTCCAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGCAGT TTCTTGATTC TGATACTCCT 
AGGACATTCT ATCACTTTGA GGTGGCCTGG GACAGCTCTA TGCACAACTC TTTGTTGCTG 
AATCGTGTCA CTCCATACAG AGAGAAGATC TACATGACAT TGTCTGCCTA TGTTGAGATG 
GAAAATTGTA CACAGCCTGC TGTGATCACC AAAGACTTCT GCATGGTTTT CTATTCTCGA 
GATGCCAAAC TCCCAGCTTG TCGCTCCATT CGGAACCTGT TTGGAACTGG CAGCTTACGA 
GCCTCTGAAA GTAATCGTGT GACTGGAGTT TATGAGATGA GTCTGTGTCG TGTTGCAGAA 
ACCAGTAGTC CAGGGATGCA GCGGAGACGG AGACGTGTGC TAGACACCTC AGTAGCATAT 
GTACGTGGGG AGGAAAACCT GGCAGGGTGG AGGCCACGGA GTGACAGCCT TATCCTTGAC 
CACCAGTGGG AACTGGAGAA ACTTAGCTTG CTTCAGGAGG TGGAGAAAAC TCGCCATTAC 
CTGCTGCTGA GAGAGAAACT GGAAGCCACA CTCAGGGGAG CCACAGAATC TCTCACCCCT 
TCCCCAATTG AGGAGATGCT GCCCCTTAGC CCAACATGTC AGCCGCTTCA GCTCACAGAG 
GTTGGATCAC CCCGTGCAGA AGAGAACCCC CTAGAAACTG CCAGCGAAAG GCAGAAAGAG 
CTGGCTGCCA AGTGTCTTCG CCTGCTCACC CACACCTTTA ACAGAGAGTA CAGTTACAGT 
CATGTATGTG TGAGTGCCAG CGAGAGTAAG CTTTCTGAAA TGTCAGTTAC ACTTTTACGG 
GACCCCTCAC TGTCTGGCTT AGGGGTCAAC TCCCTTACAC CATCCAGCAC CTGCCCTTCC 
CTAGTAGAAG GACGTTACTG CAGTCCAGAG GCTAGCCCAC AACCTATCTC CCGCGTATTC 
AGCCCTGAGA GAGAGCTGCT GAGCCCAGCC GGAGACCTAC TGAAATCTCC CAGTGGTGAA 
CTGAAGACTC CCACTCTAAA CCTGTTTGTA CCAGAAATAC AAGAAGTCAG AGTTAGTCCA 
ATTGTCTCTA AGAAGGGATA CCTACACTTC CTGGAGCCAC ATACAGGAGG CTGGGTGAAG 
AGATATGTAG TAGTCAGGCG ACCCTATGTC TACATTTACA ATAGCGATCG GGACCCAGTG 
GAGAGAGCCA TCCTTAACCT CTCCCAGGCC CATGTTGAAT ATAGTGAGGA CCAACAAGCT 
ATGCTGAAGA CCCCCAACAC TTTTGCAGTA TGCACACAGC ACCGCGGCAT CCTGCTGCAG 
GCCAGCAATG ACAAAGACAT GCACGACTGG CTCTACGCCT TTAACCCTCT CCTGGCGGGC 
TCCATCCGGT CCAAACTGTC CAGGCGCAGA TCAGCCCAAA TGAGAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826722.1
CDS	216..5306
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDTVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRQFLDSDTPRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_012971268.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971268.3

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
TTCCAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGCAGT TTCTTGATTC TGATACTCCT 
AGGACATTCT ATCACTTTGA GGTGGCCTGG GACAGCTCTA TGCACAACTC TTTGTTGCTG 
AATCGTGTCA CTCCATACAG AGAGAAGATC TACATGACAT TGTCTGCCTA TGTTGAGATG 
GAAAATTGTA CACAGCCTGC TGTGATCACC AAAGACTTCT GCATGGTTTT CTATTCTCGA 
GATGCCAAAC TCCCAGCTTG TCGCTCCATT CGGAACCTGT TTGGAACTGG CAGCTTACGA 
GCCTCTGAAA GTAATCGTGT GACTGGAGTT TATGAGATGA GTCTGTGTCG TGTTGCAGAA 
ACCAGTAGTC CAGGGATGCA GCGGAGACGG AGACGTGTGC TAGACACCTC AGTAGCATAT 
GTACGTGGGG AGGAAAACCT GGCAGGGTGG AGGCCACGGA GTGACAGCCT TATCCTTGAC 
CACCAGTGGG AACTGGAGAA ACTTAGCTTG CTTCAGGAGG TGGAGAAAAC TCGCCATTAC 
CTGCTGCTGA GAGAGAAACT GGAAGCCACA CTCAGGGGAG CCACAGAATC TCTCACCCCT 
TCCCCAATTG AGGAGATGCT GCCCCTTAGC CCAACATGTC AGCCGCTTCA GCTCACAGAG 
GTTGGATCAC CCCGTGCAGA AGAGAACCCC CTAGAAACTG CCAGCGAAAG GCAGAAAGAG 
CTGGCTGCCA AGTGTCTTCG CCTGCTCACC CACACCTTTA ACAGAGAGTA CAGTTACAGT 
CATGTATGTG TGAGTGCCAG CGAGAGTAAG CTTTCTGAAA TGTCAGTTAC ACTTTTACGG 
GACCCCTCAC TGTCTGGCTT AGGGGTCAAC TCCCTTACAC CATCCAGCAC CTGCCCTTCC 
CTAGTAGAAG GACGTTACTG CAGTCCAGAG GCTAGCCCAC AACCTATCTC CCGCGTATTC 
AGCCCTGAGA GAGAGCTGCT GAGCCCAGCC GGAGACCTAC TGAAATCTCC CAGTGGTGAA 
CTGAAGACTC CCACTCTAAA CCTGTTTGTA CCAGAAATAC AAGAAGTCAG AGTTAGTCCA 
ATTGTCTCTA AGAAGGGATA CCTACACTTC CTGGAGCCAC ATACAGGAGG CTGGGTGAAG 
AGATATGTAG TAGTCAGGCG ACCCTATGTC TACATTTACA ATAGCGATCG GGACCCAGTG 
GAGAGAGCCA TCCTTAACCT CTCCCAGGCC CATGTTGAAT ATAGTGAGGA CCAACAAGCT 
ATGCTGAAGA CCCCCAACAC TTTTGCAGTA TGCACACAGC ACCGCGGCAT CCTGCTGCAG 
GCCAGCAATG ACAAAGACAT GCACGACTGG CTCTACGCCT TTAACCCTCT CCTGGCGGGC 
TCCATCCGGT CCAAACTGTC CAGGCGCAGA TCAGCCCAAA TGAGAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_012971269.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826723.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X12, mRNA.

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CloneID	OXa30862
Clone ID Related Accession (Same CDS sequence)	XM_012971269.3 , XM_012971269.2
Accession Version	XM_012971269.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971269.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
TTCCAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGACAT TCTATCACTT TGAGGTGGCC 
TGGGACAGCT CTATGCACAA CTCTTTGTTG CTGAATCGTG TCACTCCATA CAGAGAGAAG 
ATCTACATGA CATTGTCTGC CTATGTTGAG ATGGAAAATT GTACACAGCC TGCTGTGATC 
ACCAAAGACT TCTGCATGGT TTTCTATTCT CGAGATGCCA AACTCCCAGC TTGTCGCTCC 
ATTCGGAACC TGTTTGGAAC TGGCAGCTTA CGAGCCTCTG AAAGTAATCG TGTGACTGGA 
GTTTATGAGA TGAGTCTGTG TCGTGTTGCA GAAACCAGTA GTCCAGGGAT GCAGCGGAGA 
CGGAGACGTG TGCTAGACAC CTCAGTAGCA TATGTACGTG GGGAGGAAAA CCTGGCAGGG 
TGGAGGCCAC GGAGTGACAG CCTTATCCTT GACCACCAGT GGGAACTGGA GAAACTTAGC 
TTGCTTCAGG AGGTGGAGAA AACTCGCCAT TACCTGCTGC TGAGAGAGAA ACTGGAAGCC 
ACACTCAGGG GAGCCACAGA ATCTCTCACC CCTTCCCCAA TTGAGGAGAT GCTGCCCCTT 
AGCCCAACAT GTCAGCCGCT TCAGCTCACA GAGGTTGGAT CACCCCGTGC AGAAGAGAAC 
CCCCTAGAAA CTGCCAGCGA AAGGCAGAAA GAGCTGGCTG CCAAGTGTCT TCGCCTGCTC 
ACCCACACCT TTAACAGAGA GTACAGTTAC AGTCATGTAT GTGTGAGTGC CAGCGAGAGT 
AAGCTTTCTG AAATGTCAGT TACACTTTTA CGGGACCCCT CACTGTCTGG CTTAGGGGTC 
AACTCCCTTA CACCATCCAG CACCTGCCCT TCCCTAGTAG AAGGACGTTA CTGCAGTCCA 
GAGGCTAGCC CACAACCTAT CTCCCGCGTA TTCAGCCCTG AGAGAGAGCT GCTGAGCCCA 
GCCGGAGACC TACTGAAATC TCCCAGTGGT GAACTGAAGA CTCCCACTCT AAACCTGTTT 
GTACCAGAAA TACAAGAAGT CAGAGTTAGT CCAATTGTCT CTAAGAAGGG ATACCTACAC 
TTCCTGGAGC CACATACAGG AGGCTGGGTG AAGAGATATG TAGTAGTCAG GCGACCCTAT 
GTCTACATTT ACAATAGCGA TCGGGACCCA GTGGAGAGAG CCATCCTTAA CCTCTCCCAG 
GCCCATGTTG AATATAGTGA GGACCAACAA GCTATGCTGA AGACCCCCAA CACTTTTGCA 
GTATGCACAC AGCACCGCGG CATCCTGCTG CAGGCCAGCA ATGACAAAGA CATGCACGAC 
TGGCTCTACG CCTTTAACCC TCTCCTGGCG GGCTCCATCC GGTCCAAACT GTCCAGGCGC 
AGATCAGCCC AAATGAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826723.1
CDS	216..5279
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDTVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_012971269.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971269.3

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
TTCCAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGACAT TCTATCACTT TGAGGTGGCC 
TGGGACAGCT CTATGCACAA CTCTTTGTTG CTGAATCGTG TCACTCCATA CAGAGAGAAG 
ATCTACATGA CATTGTCTGC CTATGTTGAG ATGGAAAATT GTACACAGCC TGCTGTGATC 
ACCAAAGACT TCTGCATGGT TTTCTATTCT CGAGATGCCA AACTCCCAGC TTGTCGCTCC 
ATTCGGAACC TGTTTGGAAC TGGCAGCTTA CGAGCCTCTG AAAGTAATCG TGTGACTGGA 
GTTTATGAGA TGAGTCTGTG TCGTGTTGCA GAAACCAGTA GTCCAGGGAT GCAGCGGAGA 
CGGAGACGTG TGCTAGACAC CTCAGTAGCA TATGTACGTG GGGAGGAAAA CCTGGCAGGG 
TGGAGGCCAC GGAGTGACAG CCTTATCCTT GACCACCAGT GGGAACTGGA GAAACTTAGC 
TTGCTTCAGG AGGTGGAGAA AACTCGCCAT TACCTGCTGC TGAGAGAGAA ACTGGAAGCC 
ACACTCAGGG GAGCCACAGA ATCTCTCACC CCTTCCCCAA TTGAGGAGAT GCTGCCCCTT 
AGCCCAACAT GTCAGCCGCT TCAGCTCACA GAGGTTGGAT CACCCCGTGC AGAAGAGAAC 
CCCCTAGAAA CTGCCAGCGA AAGGCAGAAA GAGCTGGCTG CCAAGTGTCT TCGCCTGCTC 
ACCCACACCT TTAACAGAGA GTACAGTTAC AGTCATGTAT GTGTGAGTGC CAGCGAGAGT 
AAGCTTTCTG AAATGTCAGT TACACTTTTA CGGGACCCCT CACTGTCTGG CTTAGGGGTC 
AACTCCCTTA CACCATCCAG CACCTGCCCT TCCCTAGTAG AAGGACGTTA CTGCAGTCCA 
GAGGCTAGCC CACAACCTAT CTCCCGCGTA TTCAGCCCTG AGAGAGAGCT GCTGAGCCCA 
GCCGGAGACC TACTGAAATC TCCCAGTGGT GAACTGAAGA CTCCCACTCT AAACCTGTTT 
GTACCAGAAA TACAAGAAGT CAGAGTTAGT CCAATTGTCT CTAAGAAGGG ATACCTACAC 
TTCCTGGAGC CACATACAGG AGGCTGGGTG AAGAGATATG TAGTAGTCAG GCGACCCTAT 
GTCTACATTT ACAATAGCGA TCGGGACCCA GTGGAGAGAG CCATCCTTAA CCTCTCCCAG 
GCCCATGTTG AATATAGTGA GGACCAACAA GCTATGCTGA AGACCCCCAA CACTTTTGCA 
GTATGCACAC AGCACCGCGG CATCCTGCTG CAGGCCAGCA ATGACAAAGA CATGCACGAC 
TGGCTCTACG CCTTTAACCC TCTCCTGGCG GGCTCCATCC GGTCCAAACT GTCCAGGCGC 
AGATCAGCCC AAATGAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_018094451.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949940.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa44798
Clone ID Related Accession (Same CDS sequence)	XM_018094451.1
Accession Version	XM_018094451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GGGCTTTTGT GTACCTGAGT 
AATTTGCTGT ACTCTGTCCC TTTGGTTCAT CGAGTCGCCA TTGTCAGTGA GAAAGGAGAA 
GTGAAAGGAT TTCTTCGTGT TGCTGTTCAG GCCATATCAG CTGACGAGGA AGCTCCTGAT 
TATGGTTCTG GAGTCCGGCA GTCTGGCACA GCTAAAATAT CCTTTGATGA TCAACATTTT 
CAAAAGTTCC AGGCTGAAAC CTGCCCAGGA ATGACACGCT CAGGCACTTC ACAAGAAGAG 
CTTCGAATTG TTGAGGGAGG ACAAGGACAA AACCCGGACT CGTGTCCTTC TGCTGACGAA 
GTGAACAACA ATACCTGTGC AGCTTCTCTG CAGTCTTCCT TGTTGGATAG TCCTACCAAG 
CCACCCTTTG ACCTCTCAGA CACTATGGGC CACCCTCTGA AACTGGGCAG CATCTTCACA 
TTCAGAGTGA CTGTCTTGCA GGCATCGAGT ATACCTCCAG AATATGCTGA CATATTCTGC 
CAATTCAATT TTATTCATCG CCATGATGAG GCCTTTTCAA CTGAACCATT AAAAAACACA 
GGCCGAGGGC CACCACTGGG CTTCTACCAT GTGCAAAATA TTGCTGTGGA GGTCACTCAG 
TCATTTGTGG AATATGTGAA GAGTCAGCCA ATTGTCTTTG AGGTGTTTGG ACATTATCAG 
CAGCATCCTT TCCCTCCTCT GTGCAAAGAT GTTCTAAGCC CTCTGCGCTC TTCCAGACGC 
CTGCTTCCAA GGGTCATGCC TCTGTCTAAA CCAGTTCCTG CCACTAAGTT GAGCAGACAG 
CGAGTTCCCA GTGTTGGTCC CTGTCACTGC AAGTATGACC TGATGGCTTA CTTCCAGATC 
TGTGAACTTG AAGCCAATGG AGATTACATA CCTGTGTCAG TAGATCATGG AGTGGGTTTG 
CCTTGTCATG GGACATTTCT TCTCCACCAG GGTATCCAGA GACGCATTTC AGTGACACTG 
GTGCATGAAG CTGGCGCTGA GCTGAAGTGG CTGGAAGTGC GAGAGCTGGT TGTAGGTCGC 
ATCCGGAATA CCCCAGAGGC AGATGAGTCT CTAATTGACC CAAACATCTT GTCACTGAAT 
ATTCTATCTG CGGGATATCG GCCCCCTGAA GGAGGTGATC GGCAGTTTCT TGATTCTGAT 
ACTCCTAGGA CATTCTATCA CTTTGAGGTG GCCTGGGACA GCTCTATGCA CAACTCTTTG 
TTGCTGAATC GTGTCACTCC ATACAGAGAG AAGATCTACA TGACATTGTC TGCCTATGTT 
GAGATGGAAA ATTGTACACA GCCTGCTGTG ATCACCAAAG ACTTCTGCAT GGTTTTCTAT 
TCTCGAGATG CCAAACTCCC AGCTTGTCGC TCCATTCGGA ACCTGTTTGG AACTGGCAGC 
TTACGAGCCT CTGAAAGTAA TCGTGTGACT GGAGTTTATG AGATGAGTCT GTGTCGTGTT 
GCAGAAACCA GTAGTCCAGG GATGCAGCGG AGACGGAGAC GTGTGCTAGA CACCTCAGTA 
GCATATGTAC GTGGGGAGGA AAACCTGGCA GGGTGGAGGC CACGGAGTGA CAGCCTTATC 
CTTGACCACC AGTGGGAACT GGAGAAACTT AGCTTGCTTC AGGAGGTGGA GAAAACTCGC 
CATTACCTGC TGCTGAGAGA GAAACTGGAA GCCACACTCA GGGGAGCCAC AGAATCTCTC 
ACCCCTTCCC CAATTGAGGA GATGCTGCCC CTTAGCCCAA CATGTCAGCC GCTTCAGCTC 
ACAGAGGTTG GATCACCCCG TGCAGAAGAG AACCCCCTAG AAACTGCCAG CGAAAGGCAG 
AAAGAGCTGG CTGCCAAGTG TCTTCGCCTG CTCACCCACA CCTTTAACAG AGAGTACAGT 
TACAGTCATG TATGTGTGAG TGCCAGCGAG AGTAAGCTTT CTGAAATGTC AGTTACACTT 
TTACGGGACC CCTCACTGTC TGGCTTAGGG GTCAACTCCC TTACACCATC CAGCACCTGC 
CCTTCCCTAG TAGAAGGACG TTACTGCAGT CCAGAGGCTA GCCCACAACC TATCTCCCGC 
GTATTCAGCC CTGAGAGAGA GCTGCTGAGC CCAGCCGGAG ACCTACTGAA ATCTCCCAGT 
GGTGAACTGA AGACTCCCAC TCTAAACCTG TTTGTACCAG AAATACAAGA AGTCAGAGTT 
AGTCCAATTG TCTCTAAGAA GGGATACCTA CACTTCCTGG AGCCACATAC AGGAGGCTGG 
GTGAAGAGAT ATGTAGTAGT CAGGCGACCC TATGTCTACA TTTACAATAG CGATCGGGAC 
CCAGTGGAGA GAGCCATCCT TAACCTCTCC CAGGCCCATG TTGAATATAG TGAGGACCAA 
CAAGCTATGC TGAAGACCCC CAACACTTTT GCAGTATGCA CACAGCACCG CGGCATCCTG 
CTGCAGGCCA GCAATGACAA AGACATGCAC GACTGGCTCT ACGCCTTTAA CCCTCTCCTG 
GCGGGCTCCA TCCGGTCCAA ACTGTCCAGG CGCAGATCAG CCCAAATGAG AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949940.1
CDS	222..5378
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRQFLDSDTPRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_018094451.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094451.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GGGCTTTTGT GTACCTGAGT 
AATTTGCTGT ACTCTGTCCC TTTGGTTCAT CGAGTCGCCA TTGTCAGTGA GAAAGGAGAA 
GTGAAAGGAT TTCTTCGTGT TGCTGTTCAG GCCATATCAG CTGACGAGGA AGCTCCTGAT 
TATGGTTCTG GAGTCCGGCA GTCTGGCACA GCTAAAATAT CCTTTGATGA TCAACATTTT 
CAAAAGTTCC AGGCTGAAAC CTGCCCAGGA ATGACACGCT CAGGCACTTC ACAAGAAGAG 
CTTCGAATTG TTGAGGGAGG ACAAGGACAA AACCCGGACT CGTGTCCTTC TGCTGACGAA 
GTGAACAACA ATACCTGTGC AGCTTCTCTG CAGTCTTCCT TGTTGGATAG TCCTACCAAG 
CCACCCTTTG ACCTCTCAGA CACTATGGGC CACCCTCTGA AACTGGGCAG CATCTTCACA 
TTCAGAGTGA CTGTCTTGCA GGCATCGAGT ATACCTCCAG AATATGCTGA CATATTCTGC 
CAATTCAATT TTATTCATCG CCATGATGAG GCCTTTTCAA CTGAACCATT AAAAAACACA 
GGCCGAGGGC CACCACTGGG CTTCTACCAT GTGCAAAATA TTGCTGTGGA GGTCACTCAG 
TCATTTGTGG AATATGTGAA GAGTCAGCCA ATTGTCTTTG AGGTGTTTGG ACATTATCAG 
CAGCATCCTT TCCCTCCTCT GTGCAAAGAT GTTCTAAGCC CTCTGCGCTC TTCCAGACGC 
CTGCTTCCAA GGGTCATGCC TCTGTCTAAA CCAGTTCCTG CCACTAAGTT GAGCAGACAG 
CGAGTTCCCA GTGTTGGTCC CTGTCACTGC AAGTATGACC TGATGGCTTA CTTCCAGATC 
TGTGAACTTG AAGCCAATGG AGATTACATA CCTGTGTCAG TAGATCATGG AGTGGGTTTG 
CCTTGTCATG GGACATTTCT TCTCCACCAG GGTATCCAGA GACGCATTTC AGTGACACTG 
GTGCATGAAG CTGGCGCTGA GCTGAAGTGG CTGGAAGTGC GAGAGCTGGT TGTAGGTCGC 
ATCCGGAATA CCCCAGAGGC AGATGAGTCT CTAATTGACC CAAACATCTT GTCACTGAAT 
ATTCTATCTG CGGGATATCG GCCCCCTGAA GGAGGTGATC GGCAGTTTCT TGATTCTGAT 
ACTCCTAGGA CATTCTATCA CTTTGAGGTG GCCTGGGACA GCTCTATGCA CAACTCTTTG 
TTGCTGAATC GTGTCACTCC ATACAGAGAG AAGATCTACA TGACATTGTC TGCCTATGTT 
GAGATGGAAA ATTGTACACA GCCTGCTGTG ATCACCAAAG ACTTCTGCAT GGTTTTCTAT 
TCTCGAGATG CCAAACTCCC AGCTTGTCGC TCCATTCGGA ACCTGTTTGG AACTGGCAGC 
TTACGAGCCT CTGAAAGTAA TCGTGTGACT GGAGTTTATG AGATGAGTCT GTGTCGTGTT 
GCAGAAACCA GTAGTCCAGG GATGCAGCGG AGACGGAGAC GTGTGCTAGA CACCTCAGTA 
GCATATGTAC GTGGGGAGGA AAACCTGGCA GGGTGGAGGC CACGGAGTGA CAGCCTTATC 
CTTGACCACC AGTGGGAACT GGAGAAACTT AGCTTGCTTC AGGAGGTGGA GAAAACTCGC 
CATTACCTGC TGCTGAGAGA GAAACTGGAA GCCACACTCA GGGGAGCCAC AGAATCTCTC 
ACCCCTTCCC CAATTGAGGA GATGCTGCCC CTTAGCCCAA CATGTCAGCC GCTTCAGCTC 
ACAGAGGTTG GATCACCCCG TGCAGAAGAG AACCCCCTAG AAACTGCCAG CGAAAGGCAG 
AAAGAGCTGG CTGCCAAGTG TCTTCGCCTG CTCACCCACA CCTTTAACAG AGAGTACAGT 
TACAGTCATG TATGTGTGAG TGCCAGCGAG AGTAAGCTTT CTGAAATGTC AGTTACACTT 
TTACGGGACC CCTCACTGTC TGGCTTAGGG GTCAACTCCC TTACACCATC CAGCACCTGC 
CCTTCCCTAG TAGAAGGACG TTACTGCAGT CCAGAGGCTA GCCCACAACC TATCTCCCGC 
GTATTCAGCC CTGAGAGAGA GCTGCTGAGC CCAGCCGGAG ACCTACTGAA ATCTCCCAGT 
GGTGAACTGA AGACTCCCAC TCTAAACCTG TTTGTACCAG AAATACAAGA AGTCAGAGTT 
AGTCCAATTG TCTCTAAGAA GGGATACCTA CACTTCCTGG AGCCACATAC AGGAGGCTGG 
GTGAAGAGAT ATGTAGTAGT CAGGCGACCC TATGTCTACA TTTACAATAG CGATCGGGAC 
CCAGTGGAGA GAGCCATCCT TAACCTCTCC CAGGCCCATG TTGAATATAG TGAGGACCAA 
CAAGCTATGC TGAAGACCCC CAACACTTTT GCAGTATGCA CACAGCACCG CGGCATCCTG 
CTGCAGGCCA GCAATGACAA AGACATGCAC GACTGGCTCT ACGCCTTTAA CCCTCTCCTG 
GCGGGCTCCA TCCGGTCCAA ACTGTCCAGG CGCAGATCAG CCCAAATGAG AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_018094452.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949941.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X2, mRNA.

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CloneID	OXa44799
Clone ID Related Accession (Same CDS sequence)	XM_018094452.1
Accession Version	XM_018094452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GGGCTTTTGT GTACCTGAGT 
AATTTGCTGT ACTCTGTCCC TTTGGTTCAT CGAGTCGCCA TTGTCAGTGA GAAAGGAGAA 
GTGAAAGGAT TTCTTCGTGT TGCTGTTCAG GCCATATCAG CTGACGAGGA AGCTCCTGAT 
TATGGTTCTG GAGTCCGGCA GTCTGGCACA GCTAAAATAT CCTTTGATGA TCAACATTTT 
CAAAAGTTCC AGGCTGAAAC CTGCCCAGGA ATGACACGCT CAGGCACTTC ACAAGAAGAG 
CTTCGAATTG TTGAGGGAGG ACAAGGACAA AACCCGGACT CGTGTCCTTC TGCTGACGAA 
GTGAACAACA ATACCTGTGC AGCTTCTCTG CAGTCTTCCT TGTTGGATAG TCCTACCAAG 
CCACCCTTTG ACCTCTCAGA CACTATGGGC CACCCTCTGA AACTGGGCAG CATCTTCACA 
TTCAGAGTGA CTGTCTTGCA GGCATCGAGT ATACCTCCAG AATATGCTGA CATATTCTGC 
CAATTCAATT TTATTCATCG CCATGATGAG GCCTTTTCAA CTGAACCATT AAAAAACACA 
GGCCGAGGGC CACCACTGGG CTTCTACCAT GTGCAAAATA TTGCTGTGGA GGTCACTCAG 
TCATTTGTGG AATATGTGAA GAGTCAGCCA ATTGTCTTTG AGGTGTTTGG ACATTATCAG 
CAGCATCCTT TCCCTCCTCT GTGCAAAGAT GTTCTAAGCC CTCTGCGCTC TTCCAGACGC 
CTGCTTCCAA GGGTCATGCC TCTGTCTAAA CCAGTTCCTG CCACTAAGTT GAGCAGACAG 
CGAGTTCCCA GTGTTGGTCC CTGTCACTGC AAGTATGACC TGATGGCTTA CTTCCAGATC 
TGTGAACTTG AAGCCAATGG AGATTACATA CCTGTGTCAG TAGATCATGG AGTGGGTTTG 
CCTTGTCATG GGACATTTCT TCTCCACCAG GGTATCCAGA GACGCATTTC AGTGACACTG 
GTGCATGAAG CTGGCGCTGA GCTGAAGTGG CTGGAAGTGC GAGAGCTGGT TGTAGGTCGC 
ATCCGGAATA CCCCAGAGGC AGATGAGTCT CTAATTGACC CAAACATCTT GTCACTGAAT 
ATTCTATCTG CGGGATATCG GCCCCCTGAA GGAGGTGATC GCTGCCAATC CGGGAGGGGC 
ACCAGGACAT TCTATCACTT TGAGGTGGCC TGGGACAGCT CTATGCACAA CTCTTTGTTG 
CTGAATCGTG TCACTCCATA CAGAGAGAAG ATCTACATGA CATTGTCTGC CTATGTTGAG 
ATGGAAAATT GTACACAGCC TGCTGTGATC ACCAAAGACT TCTGCATGGT TTTCTATTCT 
CGAGATGCCA AACTCCCAGC TTGTCGCTCC ATTCGGAACC TGTTTGGAAC TGGCAGCTTA 
CGAGCCTCTG AAAGTAATCG TGTGACTGGA GTTTATGAGA TGAGTCTGTG TCGTGTTGCA 
GAAACCAGTA GTCCAGGGAT GCAGCGGAGA CGGAGACGTG TGCTAGACAC CTCAGTAGCA 
TATGTACGTG GGGAGGAAAA CCTGGCAGGG TGGAGGCCAC GGAGTGACAG CCTTATCCTT 
GACCACCAGT GGGAACTGGA GAAACTTAGC TTGCTTCAGG AGGTGGAGAA AACTCGCCAT 
TACCTGCTGC TGAGAGAGAA ACTGGAAGCC ACACTCAGGG GAGCCACAGA ATCTCTCACC 
CCTTCCCCAA TTGAGGAGAT GCTGCCCCTT AGCCCAACAT GTCAGCCGCT TCAGCTCACA 
GAGGTTGGAT CACCCCGTGC AGAAGAGAAC CCCCTAGAAA CTGCCAGCGA AAGGCAGAAA 
GAGCTGGCTG CCAAGTGTCT TCGCCTGCTC ACCCACACCT TTAACAGAGA GTACAGTTAC 
AGTCATGTAT GTGTGAGTGC CAGCGAGAGT AAGCTTTCTG AAATGTCAGT TACACTTTTA 
CGGGACCCCT CACTGTCTGG CTTAGGGGTC AACTCCCTTA CACCATCCAG CACCTGCCCT 
TCCCTAGTAG AAGGACGTTA CTGCAGTCCA GAGGCTAGCC CACAACCTAT CTCCCGCGTA 
TTCAGCCCTG AGAGAGAGCT GCTGAGCCCA GCCGGAGACC TACTGAAATC TCCCAGTGGT 
GAACTGAAGA CTCCCACTCT AAACCTGTTT GTACCAGAAA TACAAGAAGT CAGAGTTAGT 
CCAATTGTCT CTAAGAAGGG ATACCTACAC TTCCTGGAGC CACATACAGG AGGCTGGGTG 
AAGAGATATG TAGTAGTCAG GCGACCCTAT GTCTACATTT ACAATAGCGA TCGGGACCCA 
GTGGAGAGAG CCATCCTTAA CCTCTCCCAG GCCCATGTTG AATATAGTGA GGACCAACAA 
GCTATGCTGA AGACCCCCAA CACTTTTGCA GTATGCACAC AGCACCGCGG CATCCTGCTG 
CAGGCCAGCA ATGACAAAGA CATGCACGAC TGGCTCTACG CCTTTAACCC TCTCCTGGCG 
GGCTCCATCC GGTCCAAACT GTCCAGGCGC AGATCAGCCC AAATGAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949941.1
CDS	222..5375
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRCQSGRGTRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_018094452.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094452.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GGGCTTTTGT GTACCTGAGT 
AATTTGCTGT ACTCTGTCCC TTTGGTTCAT CGAGTCGCCA TTGTCAGTGA GAAAGGAGAA 
GTGAAAGGAT TTCTTCGTGT TGCTGTTCAG GCCATATCAG CTGACGAGGA AGCTCCTGAT 
TATGGTTCTG GAGTCCGGCA GTCTGGCACA GCTAAAATAT CCTTTGATGA TCAACATTTT 
CAAAAGTTCC AGGCTGAAAC CTGCCCAGGA ATGACACGCT CAGGCACTTC ACAAGAAGAG 
CTTCGAATTG TTGAGGGAGG ACAAGGACAA AACCCGGACT CGTGTCCTTC TGCTGACGAA 
GTGAACAACA ATACCTGTGC AGCTTCTCTG CAGTCTTCCT TGTTGGATAG TCCTACCAAG 
CCACCCTTTG ACCTCTCAGA CACTATGGGC CACCCTCTGA AACTGGGCAG CATCTTCACA 
TTCAGAGTGA CTGTCTTGCA GGCATCGAGT ATACCTCCAG AATATGCTGA CATATTCTGC 
CAATTCAATT TTATTCATCG CCATGATGAG GCCTTTTCAA CTGAACCATT AAAAAACACA 
GGCCGAGGGC CACCACTGGG CTTCTACCAT GTGCAAAATA TTGCTGTGGA GGTCACTCAG 
TCATTTGTGG AATATGTGAA GAGTCAGCCA ATTGTCTTTG AGGTGTTTGG ACATTATCAG 
CAGCATCCTT TCCCTCCTCT GTGCAAAGAT GTTCTAAGCC CTCTGCGCTC TTCCAGACGC 
CTGCTTCCAA GGGTCATGCC TCTGTCTAAA CCAGTTCCTG CCACTAAGTT GAGCAGACAG 
CGAGTTCCCA GTGTTGGTCC CTGTCACTGC AAGTATGACC TGATGGCTTA CTTCCAGATC 
TGTGAACTTG AAGCCAATGG AGATTACATA CCTGTGTCAG TAGATCATGG AGTGGGTTTG 
CCTTGTCATG GGACATTTCT TCTCCACCAG GGTATCCAGA GACGCATTTC AGTGACACTG 
GTGCATGAAG CTGGCGCTGA GCTGAAGTGG CTGGAAGTGC GAGAGCTGGT TGTAGGTCGC 
ATCCGGAATA CCCCAGAGGC AGATGAGTCT CTAATTGACC CAAACATCTT GTCACTGAAT 
ATTCTATCTG CGGGATATCG GCCCCCTGAA GGAGGTGATC GCTGCCAATC CGGGAGGGGC 
ACCAGGACAT TCTATCACTT TGAGGTGGCC TGGGACAGCT CTATGCACAA CTCTTTGTTG 
CTGAATCGTG TCACTCCATA CAGAGAGAAG ATCTACATGA CATTGTCTGC CTATGTTGAG 
ATGGAAAATT GTACACAGCC TGCTGTGATC ACCAAAGACT TCTGCATGGT TTTCTATTCT 
CGAGATGCCA AACTCCCAGC TTGTCGCTCC ATTCGGAACC TGTTTGGAAC TGGCAGCTTA 
CGAGCCTCTG AAAGTAATCG TGTGACTGGA GTTTATGAGA TGAGTCTGTG TCGTGTTGCA 
GAAACCAGTA GTCCAGGGAT GCAGCGGAGA CGGAGACGTG TGCTAGACAC CTCAGTAGCA 
TATGTACGTG GGGAGGAAAA CCTGGCAGGG TGGAGGCCAC GGAGTGACAG CCTTATCCTT 
GACCACCAGT GGGAACTGGA GAAACTTAGC TTGCTTCAGG AGGTGGAGAA AACTCGCCAT 
TACCTGCTGC TGAGAGAGAA ACTGGAAGCC ACACTCAGGG GAGCCACAGA ATCTCTCACC 
CCTTCCCCAA TTGAGGAGAT GCTGCCCCTT AGCCCAACAT GTCAGCCGCT TCAGCTCACA 
GAGGTTGGAT CACCCCGTGC AGAAGAGAAC CCCCTAGAAA CTGCCAGCGA AAGGCAGAAA 
GAGCTGGCTG CCAAGTGTCT TCGCCTGCTC ACCCACACCT TTAACAGAGA GTACAGTTAC 
AGTCATGTAT GTGTGAGTGC CAGCGAGAGT AAGCTTTCTG AAATGTCAGT TACACTTTTA 
CGGGACCCCT CACTGTCTGG CTTAGGGGTC AACTCCCTTA CACCATCCAG CACCTGCCCT 
TCCCTAGTAG AAGGACGTTA CTGCAGTCCA GAGGCTAGCC CACAACCTAT CTCCCGCGTA 
TTCAGCCCTG AGAGAGAGCT GCTGAGCCCA GCCGGAGACC TACTGAAATC TCCCAGTGGT 
GAACTGAAGA CTCCCACTCT AAACCTGTTT GTACCAGAAA TACAAGAAGT CAGAGTTAGT 
CCAATTGTCT CTAAGAAGGG ATACCTACAC TTCCTGGAGC CACATACAGG AGGCTGGGTG 
AAGAGATATG TAGTAGTCAG GCGACCCTAT GTCTACATTT ACAATAGCGA TCGGGACCCA 
GTGGAGAGAG CCATCCTTAA CCTCTCCCAG GCCCATGTTG AATATAGTGA GGACCAACAA 
GCTATGCTGA AGACCCCCAA CACTTTTGCA GTATGCACAC AGCACCGCGG CATCCTGCTG 
CAGGCCAGCA ATGACAAAGA CATGCACGAC TGGCTCTACG CCTTTAACCC TCTCCTGGCG 
GGCTCCATCC GGTCCAAACT GTCCAGGCGC AGATCAGCCC AAATGAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_018094453.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949942.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X3, mRNA.

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CloneID	OXa44800
Clone ID Related Accession (Same CDS sequence)	XM_018094453.1
Accession Version	XM_018094453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GGGCTTTTGT GTACCTGAGT 
AATTTGCTGT ACTCTGTCCC TTTGGTTCAT CGAGTCGCCA TTGTCAGTGA GAAAGGAGAA 
GTGAAAGGAT TTCTTCGTGT TGCTGTTCAG GCCATATCAG CTGACGAGGA AGCTCCTGAT 
TATGGTTCTG GAGTCCGGCA GTCTGGCACA GCTAAAATAT CCTTTGATGA TCAACATTTT 
CAAAAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGCAGT TTCTTGATTC TGATACTCCT 
AGGACATTCT ATCACTTTGA GGTGGCCTGG GACAGCTCTA TGCACAACTC TTTGTTGCTG 
AATCGTGTCA CTCCATACAG AGAGAAGATC TACATGACAT TGTCTGCCTA TGTTGAGATG 
GAAAATTGTA CACAGCCTGC TGTGATCACC AAAGACTTCT GCATGGTTTT CTATTCTCGA 
GATGCCAAAC TCCCAGCTTG TCGCTCCATT CGGAACCTGT TTGGAACTGG CAGCTTACGA 
GCCTCTGAAA GTAATCGTGT GACTGGAGTT TATGAGATGA GTCTGTGTCG TGTTGCAGAA 
ACCAGTAGTC CAGGGATGCA GCGGAGACGG AGACGTGTGC TAGACACCTC AGTAGCATAT 
GTACGTGGGG AGGAAAACCT GGCAGGGTGG AGGCCACGGA GTGACAGCCT TATCCTTGAC 
CACCAGTGGG AACTGGAGAA ACTTAGCTTG CTTCAGGAGG TGGAGAAAAC TCGCCATTAC 
CTGCTGCTGA GAGAGAAACT GGAAGCCACA CTCAGGGGAG CCACAGAATC TCTCACCCCT 
TCCCCAATTG AGGAGATGCT GCCCCTTAGC CCAACATGTC AGCCGCTTCA GCTCACAGAG 
GTTGGATCAC CCCGTGCAGA AGAGAACCCC CTAGAAACTG CCAGCGAAAG GCAGAAAGAG 
CTGGCTGCCA AGTGTCTTCG CCTGCTCACC CACACCTTTA ACAGAGAGTA CAGTTACAGT 
CATGTATGTG TGAGTGCCAG CGAGAGTAAG CTTTCTGAAA TGTCAGTTAC ACTTTTACGG 
GACCCCTCAC TGTCTGGCTT AGGGGTCAAC TCCCTTACAC CATCCAGCAC CTGCCCTTCC 
CTAGTAGAAG GACGTTACTG CAGTCCAGAG GCTAGCCCAC AACCTATCTC CCGCGTATTC 
AGCCCTGAGA GAGAGCTGCT GAGCCCAGCC GGAGACCTAC TGAAATCTCC CAGTGGTGAA 
CTGAAGACTC CCACTCTAAA CCTGTTTGTA CCAGAAATAC AAGAAGTCAG AGTTAGTCCA 
ATTGTCTCTA AGAAGGGATA CCTACACTTC CTGGAGCCAC ATACAGGAGG CTGGGTGAAG 
AGATATGTAG TAGTCAGGCG ACCCTATGTC TACATTTACA ATAGCGATCG GGACCCAGTG 
GAGAGAGCCA TCCTTAACCT CTCCCAGGCC CATGTTGAAT ATAGTGAGGA CCAACAAGCT 
ATGCTGAAGA CCCCCAACAC TTTTGCAGTA TGCACACAGC ACCGCGGCAT CCTGCTGCAG 
GCCAGCAATG ACAAAGACAT GCACGACTGG CTCTACGCCT TTAACCCTCT CCTGGCGGGC 
TCCATCCGGT CCAAACTGTC CAGGCGCAGA TCAGCCCAAA TGAGAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949942.1
CDS	222..5372
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRQFLDSDTPRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_018094453.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094453.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GGGCTTTTGT GTACCTGAGT 
AATTTGCTGT ACTCTGTCCC TTTGGTTCAT CGAGTCGCCA TTGTCAGTGA GAAAGGAGAA 
GTGAAAGGAT TTCTTCGTGT TGCTGTTCAG GCCATATCAG CTGACGAGGA AGCTCCTGAT 
TATGGTTCTG GAGTCCGGCA GTCTGGCACA GCTAAAATAT CCTTTGATGA TCAACATTTT 
CAAAAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGCAGT TTCTTGATTC TGATACTCCT 
AGGACATTCT ATCACTTTGA GGTGGCCTGG GACAGCTCTA TGCACAACTC TTTGTTGCTG 
AATCGTGTCA CTCCATACAG AGAGAAGATC TACATGACAT TGTCTGCCTA TGTTGAGATG 
GAAAATTGTA CACAGCCTGC TGTGATCACC AAAGACTTCT GCATGGTTTT CTATTCTCGA 
GATGCCAAAC TCCCAGCTTG TCGCTCCATT CGGAACCTGT TTGGAACTGG CAGCTTACGA 
GCCTCTGAAA GTAATCGTGT GACTGGAGTT TATGAGATGA GTCTGTGTCG TGTTGCAGAA 
ACCAGTAGTC CAGGGATGCA GCGGAGACGG AGACGTGTGC TAGACACCTC AGTAGCATAT 
GTACGTGGGG AGGAAAACCT GGCAGGGTGG AGGCCACGGA GTGACAGCCT TATCCTTGAC 
CACCAGTGGG AACTGGAGAA ACTTAGCTTG CTTCAGGAGG TGGAGAAAAC TCGCCATTAC 
CTGCTGCTGA GAGAGAAACT GGAAGCCACA CTCAGGGGAG CCACAGAATC TCTCACCCCT 
TCCCCAATTG AGGAGATGCT GCCCCTTAGC CCAACATGTC AGCCGCTTCA GCTCACAGAG 
GTTGGATCAC CCCGTGCAGA AGAGAACCCC CTAGAAACTG CCAGCGAAAG GCAGAAAGAG 
CTGGCTGCCA AGTGTCTTCG CCTGCTCACC CACACCTTTA ACAGAGAGTA CAGTTACAGT 
CATGTATGTG TGAGTGCCAG CGAGAGTAAG CTTTCTGAAA TGTCAGTTAC ACTTTTACGG 
GACCCCTCAC TGTCTGGCTT AGGGGTCAAC TCCCTTACAC CATCCAGCAC CTGCCCTTCC 
CTAGTAGAAG GACGTTACTG CAGTCCAGAG GCTAGCCCAC AACCTATCTC CCGCGTATTC 
AGCCCTGAGA GAGAGCTGCT GAGCCCAGCC GGAGACCTAC TGAAATCTCC CAGTGGTGAA 
CTGAAGACTC CCACTCTAAA CCTGTTTGTA CCAGAAATAC AAGAAGTCAG AGTTAGTCCA 
ATTGTCTCTA AGAAGGGATA CCTACACTTC CTGGAGCCAC ATACAGGAGG CTGGGTGAAG 
AGATATGTAG TAGTCAGGCG ACCCTATGTC TACATTTACA ATAGCGATCG GGACCCAGTG 
GAGAGAGCCA TCCTTAACCT CTCCCAGGCC CATGTTGAAT ATAGTGAGGA CCAACAAGCT 
ATGCTGAAGA CCCCCAACAC TTTTGCAGTA TGCACACAGC ACCGCGGCAT CCTGCTGCAG 
GCCAGCAATG ACAAAGACAT GCACGACTGG CTCTACGCCT TTAACCCTCT CCTGGCGGGC 
TCCATCCGGT CCAAACTGTC CAGGCGCAGA TCAGCCCAAA TGAGAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_018094454.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949943.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X4, mRNA.

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CloneID	OXa44801
Clone ID Related Accession (Same CDS sequence)	XM_018094454.1
Accession Version	XM_018094454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GGGCTTTTGT GTACCTGAGT 
AATTTGCTGT ACTCTGTCCC TTTGGTTCAT CGAGTCGCCA TTGTCAGTGA GAAAGGAGAA 
GTGAAAGGAT TTCTTCGTGT TGCTGTTCAG GCCATATCAG CTGACGAGGA AGCTCCTGAT 
TATGGTTCTG GAGTCCGGCA GTCTGGCACA GCTAAAATAT CCTTTGATGA TCAACATTTT 
CAAAAGTTCC AGGCTGAAAC CTGCCCAGGA ATGACACGCT CAGGCACTTC ACAAGAAGAG 
CTTCGAATTG TTGAGGGAGG ACAAGGACAA AACCCGGACT CGTGTCCTTC TGCTGACGAA 
GTGAACAACA ATACCTGTGC AGCTTCTCTG CAGTCTTCCT TGTTGGATAG TCCTACCAAG 
CCACCCTTTG ACCTCTCAGA CACTATGGGC CACCCTCTGA AACTGGGCAG CATCTTCACA 
TTCAGAGTGA CTGTCTTGCA GGCATCGAGT ATACCTCCAG AATATGCTGA CATATTCTGC 
CAATTCAATT TTATTCATCG CCATGATGAG GCCTTTTCAA CTGAACCATT AAAAAACACA 
GGCCGAGGGC CACCACTGGG CTTCTACCAT GTGCAAAATA TTGCTGTGGA GGTCACTCAG 
TCATTTGTGG AATATGTGAA GAGTCAGCCA ATTGTCTTTG AGGTGTTTGG ACATTATCAG 
CAGCATCCTT TCCCTCCTCT GTGCAAAGAT GTTCTAAGCC CTCTGCGCTC TTCCAGACGC 
CTGCTTCCAA GGGTCATGCC TCTGTCTAAA CCAGTTCCTG CCACTAAGTT GAGCAGACAG 
CGAGTTCCCA GTGTTGGTCC CTGTCACTGC AAGTATGACC TGATGGCTTA CTTCCAGATC 
TGTGAACTTG AAGCCAATGG AGATTACATA CCTGTGTCAG TAGATCATGG AGTGGGTTTG 
CCTTGTCATG GGACATTTCT TCTCCACCAG GGTATCCAGA GACGCATTTC AGTGACACTG 
GTGCATGAAG CTGGCGCTGA GCTGAAGTGG CTGGAAGTGC GAGAGCTGGT TGTAGGTCGC 
ATCCGGAATA CCCCAGAGGC AGATGAGTCT CTAATTGACC CAAACATCTT GTCACTGAAT 
ATTCTATCTG CGGGATATCG GCCCCCTGAA GGAGGTGATC GGACATTCTA TCACTTTGAG 
GTGGCCTGGG ACAGCTCTAT GCACAACTCT TTGTTGCTGA ATCGTGTCAC TCCATACAGA 
GAGAAGATCT ACATGACATT GTCTGCCTAT GTTGAGATGG AAAATTGTAC ACAGCCTGCT 
GTGATCACCA AAGACTTCTG CATGGTTTTC TATTCTCGAG ATGCCAAACT CCCAGCTTGT 
CGCTCCATTC GGAACCTGTT TGGAACTGGC AGCTTACGAG CCTCTGAAAG TAATCGTGTG 
ACTGGAGTTT ATGAGATGAG TCTGTGTCGT GTTGCAGAAA CCAGTAGTCC AGGGATGCAG 
CGGAGACGGA GACGTGTGCT AGACACCTCA GTAGCATATG TACGTGGGGA GGAAAACCTG 
GCAGGGTGGA GGCCACGGAG TGACAGCCTT ATCCTTGACC ACCAGTGGGA ACTGGAGAAA 
CTTAGCTTGC TTCAGGAGGT GGAGAAAACT CGCCATTACC TGCTGCTGAG AGAGAAACTG 
GAAGCCACAC TCAGGGGAGC CACAGAATCT CTCACCCCTT CCCCAATTGA GGAGATGCTG 
CCCCTTAGCC CAACATGTCA GCCGCTTCAG CTCACAGAGG TTGGATCACC CCGTGCAGAA 
GAGAACCCCC TAGAAACTGC CAGCGAAAGG CAGAAAGAGC TGGCTGCCAA GTGTCTTCGC 
CTGCTCACCC ACACCTTTAA CAGAGAGTAC AGTTACAGTC ATGTATGTGT GAGTGCCAGC 
GAGAGTAAGC TTTCTGAAAT GTCAGTTACA CTTTTACGGG ACCCCTCACT GTCTGGCTTA 
GGGGTCAACT CCCTTACACC ATCCAGCACC TGCCCTTCCC TAGTAGAAGG ACGTTACTGC 
AGTCCAGAGG CTAGCCCACA ACCTATCTCC CGCGTATTCA GCCCTGAGAG AGAGCTGCTG 
AGCCCAGCCG GAGACCTACT GAAATCTCCC AGTGGTGAAC TGAAGACTCC CACTCTAAAC 
CTGTTTGTAC CAGAAATACA AGAAGTCAGA GTTAGTCCAA TTGTCTCTAA GAAGGGATAC 
CTACACTTCC TGGAGCCACA TACAGGAGGC TGGGTGAAGA GATATGTAGT AGTCAGGCGA 
CCCTATGTCT ACATTTACAA TAGCGATCGG GACCCAGTGG AGAGAGCCAT CCTTAACCTC 
TCCCAGGCCC ATGTTGAATA TAGTGAGGAC CAACAAGCTA TGCTGAAGAC CCCCAACACT 
TTTGCAGTAT GCACACAGCA CCGCGGCATC CTGCTGCAGG CCAGCAATGA CAAAGACATG 
CACGACTGGC TCTACGCCTT TAACCCTCTC CTGGCGGGCT CCATCCGGTC CAAACTGTCC 
AGGCGCAGAT CAGCCCAAAT GAGAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949943.1
CDS	222..5351
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_018094454.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094454.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GGGCTTTTGT GTACCTGAGT 
AATTTGCTGT ACTCTGTCCC TTTGGTTCAT CGAGTCGCCA TTGTCAGTGA GAAAGGAGAA 
GTGAAAGGAT TTCTTCGTGT TGCTGTTCAG GCCATATCAG CTGACGAGGA AGCTCCTGAT 
TATGGTTCTG GAGTCCGGCA GTCTGGCACA GCTAAAATAT CCTTTGATGA TCAACATTTT 
CAAAAGTTCC AGGCTGAAAC CTGCCCAGGA ATGACACGCT CAGGCACTTC ACAAGAAGAG 
CTTCGAATTG TTGAGGGAGG ACAAGGACAA AACCCGGACT CGTGTCCTTC TGCTGACGAA 
GTGAACAACA ATACCTGTGC AGCTTCTCTG CAGTCTTCCT TGTTGGATAG TCCTACCAAG 
CCACCCTTTG ACCTCTCAGA CACTATGGGC CACCCTCTGA AACTGGGCAG CATCTTCACA 
TTCAGAGTGA CTGTCTTGCA GGCATCGAGT ATACCTCCAG AATATGCTGA CATATTCTGC 
CAATTCAATT TTATTCATCG CCATGATGAG GCCTTTTCAA CTGAACCATT AAAAAACACA 
GGCCGAGGGC CACCACTGGG CTTCTACCAT GTGCAAAATA TTGCTGTGGA GGTCACTCAG 
TCATTTGTGG AATATGTGAA GAGTCAGCCA ATTGTCTTTG AGGTGTTTGG ACATTATCAG 
CAGCATCCTT TCCCTCCTCT GTGCAAAGAT GTTCTAAGCC CTCTGCGCTC TTCCAGACGC 
CTGCTTCCAA GGGTCATGCC TCTGTCTAAA CCAGTTCCTG CCACTAAGTT GAGCAGACAG 
CGAGTTCCCA GTGTTGGTCC CTGTCACTGC AAGTATGACC TGATGGCTTA CTTCCAGATC 
TGTGAACTTG AAGCCAATGG AGATTACATA CCTGTGTCAG TAGATCATGG AGTGGGTTTG 
CCTTGTCATG GGACATTTCT TCTCCACCAG GGTATCCAGA GACGCATTTC AGTGACACTG 
GTGCATGAAG CTGGCGCTGA GCTGAAGTGG CTGGAAGTGC GAGAGCTGGT TGTAGGTCGC 
ATCCGGAATA CCCCAGAGGC AGATGAGTCT CTAATTGACC CAAACATCTT GTCACTGAAT 
ATTCTATCTG CGGGATATCG GCCCCCTGAA GGAGGTGATC GGACATTCTA TCACTTTGAG 
GTGGCCTGGG ACAGCTCTAT GCACAACTCT TTGTTGCTGA ATCGTGTCAC TCCATACAGA 
GAGAAGATCT ACATGACATT GTCTGCCTAT GTTGAGATGG AAAATTGTAC ACAGCCTGCT 
GTGATCACCA AAGACTTCTG CATGGTTTTC TATTCTCGAG ATGCCAAACT CCCAGCTTGT 
CGCTCCATTC GGAACCTGTT TGGAACTGGC AGCTTACGAG CCTCTGAAAG TAATCGTGTG 
ACTGGAGTTT ATGAGATGAG TCTGTGTCGT GTTGCAGAAA CCAGTAGTCC AGGGATGCAG 
CGGAGACGGA GACGTGTGCT AGACACCTCA GTAGCATATG TACGTGGGGA GGAAAACCTG 
GCAGGGTGGA GGCCACGGAG TGACAGCCTT ATCCTTGACC ACCAGTGGGA ACTGGAGAAA 
CTTAGCTTGC TTCAGGAGGT GGAGAAAACT CGCCATTACC TGCTGCTGAG AGAGAAACTG 
GAAGCCACAC TCAGGGGAGC CACAGAATCT CTCACCCCTT CCCCAATTGA GGAGATGCTG 
CCCCTTAGCC CAACATGTCA GCCGCTTCAG CTCACAGAGG TTGGATCACC CCGTGCAGAA 
GAGAACCCCC TAGAAACTGC CAGCGAAAGG CAGAAAGAGC TGGCTGCCAA GTGTCTTCGC 
CTGCTCACCC ACACCTTTAA CAGAGAGTAC AGTTACAGTC ATGTATGTGT GAGTGCCAGC 
GAGAGTAAGC TTTCTGAAAT GTCAGTTACA CTTTTACGGG ACCCCTCACT GTCTGGCTTA 
GGGGTCAACT CCCTTACACC ATCCAGCACC TGCCCTTCCC TAGTAGAAGG ACGTTACTGC 
AGTCCAGAGG CTAGCCCACA ACCTATCTCC CGCGTATTCA GCCCTGAGAG AGAGCTGCTG 
AGCCCAGCCG GAGACCTACT GAAATCTCCC AGTGGTGAAC TGAAGACTCC CACTCTAAAC 
CTGTTTGTAC CAGAAATACA AGAAGTCAGA GTTAGTCCAA TTGTCTCTAA GAAGGGATAC 
CTACACTTCC TGGAGCCACA TACAGGAGGC TGGGTGAAGA GATATGTAGT AGTCAGGCGA 
CCCTATGTCT ACATTTACAA TAGCGATCGG GACCCAGTGG AGAGAGCCAT CCTTAACCTC 
TCCCAGGCCC ATGTTGAATA TAGTGAGGAC CAACAAGCTA TGCTGAAGAC CCCCAACACT 
TTTGCAGTAT GCACACAGCA CCGCGGCATC CTGCTGCAGG CCAGCAATGA CAAAGACATG 
CACGACTGGC TCTACGCCTT TAACCCTCTC CTGGCGGGCT CCATCCGGTC CAAACTGTCC 
AGGCGCAGAT CAGCCCAAAT GAGAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_018094455.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949944.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X13, mRNA.

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CloneID	OXa44802
Clone ID Related Accession (Same CDS sequence)	XM_018094455.2 , XM_018094455.1
Accession Version	XM_018094455.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094455.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGCTCTC CCCTATTCAC CACATGCATG 
AGCGAGAGCA TGGCCGAAAT TACTCCCTCC CCCACTCTAT CGAGCCCCGG CTCCGAAGGC 
CCGGAGCCGG CCTCCCAGCT TGAAGAGGAG GATATTTTTC AGGATGATGA GGAAGAGGAT 
GATGGACCTC AGGAGCATCC CTGCTGTGCT GTCAGATCAG ACCCGTTTCA CGACCGATCC 
CCCCTGTTCA GTGTAGTGGG AAGGGCTTTT GTGTACCTGA GTAATTTGCT GTACTCTGTC 
CCTTTGGTTC ATCGAGTCGC CATTGTCAGT GAGAAAGGAG AAGTGAAAGG ATTTCTTCGT 
GTTGCTGTTC AGGCCATATC AGCTGACGAG GAAGCTCCTG ATTATGGTTC TGGAGTCCGG 
CAGTCTGGCA CAGCTAAAAT ATCCTTTGAT GATCAACATT TTCAAAAGTT CCAGGCTGAA 
ACCTGCCCAG GAATGACACG CTCAGGCACT TCACAAGAAG AGCTTCGAAT TGTTGAGGGA 
GGACAAGGAC AAAACCCGGA CTCGTGTCCT TCTGCTGACG AAGTGAACAA CAATACCTGT 
GCAGCTTCTC TGCAGTCTTC CTTGTTGGAT AGTCCTACCA AGCCACCCTT TGACCTCTCA 
GACACTATGG GCCACCCTCT GAAACTGGGC AGCATCTTCA CATTCAGAGT GACTGTCTTG 
CAGGCATCGA GTATACCTCC AGAATATGCT GACATATTCT GCCAATTCAA TTTTATTCAT 
CGCCATGATG AGGCCTTTTC AACTGAACCA TTAAAAAACA CAGGCCGAGG GCCACCACTG 
GGCTTCTACC ATGTGCAAAA TATTGCTGTG GAGGTCACTC AGTCATTTGT GGAATATGTG 
AAGAGTCAGC CAATTGTCTT TGAGGTGTTT GGACATTATC AGCAGCATCC TTTCCCTCCT 
CTGTGCAAAG ATGTTCTAAG CCCTCTGCGC TCTTCCAGAC GCCTGCTTCC AAGGGTCATG 
CCTCTGTCTA AACCAGTTCC TGCCACTAAG TTGAGCAGAC AGCGAGTTCC CAGTGTTGGT 
CCCTGTCACT GCAAGTATGA CCTGATGGCT TACTTCCAGA TCTGTGAACT TGAAGCCAAT 
GGAGATTACA TACCTGTGTC AGTAGATCAT GGAGTGGGTT TGCCTTGTCA TGGGACATTT 
CTTCTCCACC AGGGTATCCA GAGACGCATT TCAGTGACAC TGGTGCATGA AGCTGGCGCT 
GAGCTGAAGT GGCTGGAAGT GCGAGAGCTG GTTGTAGGTC GCATCCGGAA TACCCCAGAG 
GCAGATGAGT CTCTAATTGA CCCAAACATC TTGTCACTGA ATATTCTATC TGCGGGATAT 
CGGCCCCCTG AAGGAGGTGA TCGGACATTC TATCACTTTG AGGTGGCCTG GGACAGCTCT 
ATGCACAACT CTTTGTTGCT GAATCGTGTC ACTCCATACA GAGAGAAGAT CTACATGACA 
TTGTCTGCCT ATGTTGAGAT GGAAAATTGT ACACAGCCTG CTGTGATCAC CAAAGACTTC 
TGCATGGTTT TCTATTCTCG AGATGCCAAA CTCCCAGCTT GTCGCTCCAT TCGGAACCTG 
TTTGGAACTG GCAGCTTACG AGCCTCTGAA AGTAATCGTG TGACTGGAGT TTATGAGATG 
AGTCTGTGTC GTGTTGCAGA AACCAGTAGT CCAGGGATGC AGCGGAGACG GAGACGTGTG 
CTAGACACCT CAGTAGCATA TGTACGTGGG GAGGAAAACC TGGCAGGGTG GAGGCCACGG 
AGTGACAGCC TTATCCTTGA CCACCAGTGG GAACTGGAGA AACTTAGCTT GCTTCAGGAG 
GTGGAGAAAA CTCGCCATTA CCTGCTGCTG AGAGAGAAAC TGGAAGCCAC ACTCAGGGGA 
GCCACAGAAT CTCTCACCCC TTCCCCAATT GAGGAGATGC TGCCCCTTAG CCCAACATGT 
CAGCCGCTTC AGCTCACAGA GGTTGGATCA CCCCGTGCAG AAGAGAACCC CCTAGAAACT 
GCCAGCGAAA GGCAGAAAGA GCTGGCTGCC AAGTGTCTTC GCCTGCTCAC CCACACCTTT 
AACAGAGAGT ACAGTTACAG TCATGTATGT GTGAGTGCCA GCGAGAGTAA GCTTTCTGAA 
ATGTCAGTTA CACTTTTACG GGACCCCTCA CTGTCTGGCT TAGGGGTCAA CTCCCTTACA 
CCATCCAGCA CCTGCCCTTC CCTAGTAGAA GGACGTTACT GCAGTCCAGA GGCTAGCCCA 
CAACCTATCT CCCGCGTATT CAGCCCTGAG AGAGAGCTGC TGAGCCCAGC CGGAGACCTA 
CTGAAATCTC CCAGTGGTGA ACTGAAGACT CCCACTCTAA ACCTGTTTGT ACCAGAAATA 
CAAGAAGTCA GAGTTAGTCC AATTGTCTCT AAGAAGGGAT ACCTACACTT CCTGGAGCCA 
CATACAGGAG GCTGGGTGAA GAGATATGTA GTAGTCAGGC GACCCTATGT CTACATTTAC 
AATAGCGATC GGGACCCAGT GGAGAGAGCC ATCCTTAACC TCTCCCAGGC CCATGTTGAA 
TATAGTGAGG ACCAACAAGC TATGCTGAAG ACCCCCAACA CTTTTGCAGT ATGCACACAG 
CACCGCGGCA TCCTGCTGCA GGCCAGCAAT GACAAAGACA TGCACGACTG GCTCTACGCC 
TTTAACCCTC TCCTGGCGGG CTCCATCCGG TCCAAACTGT CCAGGCGCAG ATCAGCCCAA 
ATGAGAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949944.1
CDS	217..5508
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDTVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGSSPLFTTCMSESMAEITPSPTLSSPGSEGPEPASQLEEEDIFQDDEEEDDGPQEHPCCAVRSDPFHDRSPLFSVVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_018094455.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094455.2

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGCTCTC CCCTATTCAC CACATGCATG 
AGCGAGAGCA TGGCCGAAAT TACTCCCTCC CCCACTCTAT CGAGCCCCGG CTCCGAAGGC 
CCGGAGCCGG CCTCCCAGCT TGAAGAGGAG GATATTTTTC AGGATGATGA GGAAGAGGAT 
GATGGACCTC AGGAGCATCC CTGCTGTGCT GTCAGATCAG ACCCGTTTCA CGACCGATCC 
CCCCTGTTCA GTGTAGTGGG AAGGGCTTTT GTGTACCTGA GTAATTTGCT GTACTCTGTC 
CCTTTGGTTC ATCGAGTCGC CATTGTCAGT GAGAAAGGAG AAGTGAAAGG ATTTCTTCGT 
GTTGCTGTTC AGGCCATATC AGCTGACGAG GAAGCTCCTG ATTATGGTTC TGGAGTCCGG 
CAGTCTGGCA CAGCTAAAAT ATCCTTTGAT GATCAACATT TTCAAAAGTT CCAGGCTGAA 
ACCTGCCCAG GAATGACACG CTCAGGCACT TCACAAGAAG AGCTTCGAAT TGTTGAGGGA 
GGACAAGGAC AAAACCCGGA CTCGTGTCCT TCTGCTGACG AAGTGAACAA CAATACCTGT 
GCAGCTTCTC TGCAGTCTTC CTTGTTGGAT AGTCCTACCA AGCCACCCTT TGACCTCTCA 
GACACTATGG GCCACCCTCT GAAACTGGGC AGCATCTTCA CATTCAGAGT GACTGTCTTG 
CAGGCATCGA GTATACCTCC AGAATATGCT GACATATTCT GCCAATTCAA TTTTATTCAT 
CGCCATGATG AGGCCTTTTC AACTGAACCA TTAAAAAACA CAGGCCGAGG GCCACCACTG 
GGCTTCTACC ATGTGCAAAA TATTGCTGTG GAGGTCACTC AGTCATTTGT GGAATATGTG 
AAGAGTCAGC CAATTGTCTT TGAGGTGTTT GGACATTATC AGCAGCATCC TTTCCCTCCT 
CTGTGCAAAG ATGTTCTAAG CCCTCTGCGC TCTTCCAGAC GCCTGCTTCC AAGGGTCATG 
CCTCTGTCTA AACCAGTTCC TGCCACTAAG TTGAGCAGAC AGCGAGTTCC CAGTGTTGGT 
CCCTGTCACT GCAAGTATGA CCTGATGGCT TACTTCCAGA TCTGTGAACT TGAAGCCAAT 
GGAGATTACA TACCTGTGTC AGTAGATCAT GGAGTGGGTT TGCCTTGTCA TGGGACATTT 
CTTCTCCACC AGGGTATCCA GAGACGCATT TCAGTGACAC TGGTGCATGA AGCTGGCGCT 
GAGCTGAAGT GGCTGGAAGT GCGAGAGCTG GTTGTAGGTC GCATCCGGAA TACCCCAGAG 
GCAGATGAGT CTCTAATTGA CCCAAACATC TTGTCACTGA ATATTCTATC TGCGGGATAT 
CGGCCCCCTG AAGGAGGTGA TCGGACATTC TATCACTTTG AGGTGGCCTG GGACAGCTCT 
ATGCACAACT CTTTGTTGCT GAATCGTGTC ACTCCATACA GAGAGAAGAT CTACATGACA 
TTGTCTGCCT ATGTTGAGAT GGAAAATTGT ACACAGCCTG CTGTGATCAC CAAAGACTTC 
TGCATGGTTT TCTATTCTCG AGATGCCAAA CTCCCAGCTT GTCGCTCCAT TCGGAACCTG 
TTTGGAACTG GCAGCTTACG AGCCTCTGAA AGTAATCGTG TGACTGGAGT TTATGAGATG 
AGTCTGTGTC GTGTTGCAGA AACCAGTAGT CCAGGGATGC AGCGGAGACG GAGACGTGTG 
CTAGACACCT CAGTAGCATA TGTACGTGGG GAGGAAAACC TGGCAGGGTG GAGGCCACGG 
AGTGACAGCC TTATCCTTGA CCACCAGTGG GAACTGGAGA AACTTAGCTT GCTTCAGGAG 
GTGGAGAAAA CTCGCCATTA CCTGCTGCTG AGAGAGAAAC TGGAAGCCAC ACTCAGGGGA 
GCCACAGAAT CTCTCACCCC TTCCCCAATT GAGGAGATGC TGCCCCTTAG CCCAACATGT 
CAGCCGCTTC AGCTCACAGA GGTTGGATCA CCCCGTGCAG AAGAGAACCC CCTAGAAACT 
GCCAGCGAAA GGCAGAAAGA GCTGGCTGCC AAGTGTCTTC GCCTGCTCAC CCACACCTTT 
AACAGAGAGT ACAGTTACAG TCATGTATGT GTGAGTGCCA GCGAGAGTAA GCTTTCTGAA 
ATGTCAGTTA CACTTTTACG GGACCCCTCA CTGTCTGGCT TAGGGGTCAA CTCCCTTACA 
CCATCCAGCA CCTGCCCTTC CCTAGTAGAA GGACGTTACT GCAGTCCAGA GGCTAGCCCA 
CAACCTATCT CCCGCGTATT CAGCCCTGAG AGAGAGCTGC TGAGCCCAGC CGGAGACCTA 
CTGAAATCTC CCAGTGGTGA ACTGAAGACT CCCACTCTAA ACCTGTTTGT ACCAGAAATA 
CAAGAAGTCA GAGTTAGTCC AATTGTCTCT AAGAAGGGAT ACCTACACTT CCTGGAGCCA 
CATACAGGAG GCTGGGTGAA GAGATATGTA GTAGTCAGGC GACCCTATGT CTACATTTAC 
AATAGCGATC GGGACCCAGT GGAGAGAGCC ATCCTTAACC TCTCCCAGGC CCATGTTGAA 
TATAGTGAGG ACCAACAAGC TATGCTGAAG ACCCCCAACA CTTTTGCAGT ATGCACACAG 
CACCGCGGCA TCCTGCTGCA GGCCAGCAAT GACAAAGACA TGCACGACTG GCTCTACGCC 
TTTAACCCTC TCCTGGCGGG CTCCATCCGG TCCAAACTGT CCAGGCGCAG ATCAGCCCAA 
ATGAGAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_018094455.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949944.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X7, mRNA.

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CloneID	OXa44802
Clone ID Related Accession (Same CDS sequence)	XM_018094455.2 , XM_018094455.1
Accession Version	XM_018094455.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGCTCTC CCCTATTCAC CACATGCATG 
AGCGAGAGCA TGGCCGAAAT TACTCCCTCC CCCACTCTAT CGAGCCCCGG CTCCGAAGGC 
CCGGAGCCGG CCTCCCAGCT TGAAGAGGAG GATATTTTTC AGGATGATGA GGAAGAGGAT 
GATGGACCTC AGGAGCATCC CTGCTGTGCT GTCAGATCAG ACCCGTTTCA CGACCGATCC 
CCCCTGTTCA GTGTAGTGGG AAGGGCTTTT GTGTACCTGA GTAATTTGCT GTACTCTGTC 
CCTTTGGTTC ATCGAGTCGC CATTGTCAGT GAGAAAGGAG AAGTGAAAGG ATTTCTTCGT 
GTTGCTGTTC AGGCCATATC AGCTGACGAG GAAGCTCCTG ATTATGGTTC TGGAGTCCGG 
CAGTCTGGCA CAGCTAAAAT ATCCTTTGAT GATCAACATT TTCAAAAGTT CCAGGCTGAA 
ACCTGCCCAG GAATGACACG CTCAGGCACT TCACAAGAAG AGCTTCGAAT TGTTGAGGGA 
GGACAAGGAC AAAACCCGGA CTCGTGTCCT TCTGCTGACG AAGTGAACAA CAATACCTGT 
GCAGCTTCTC TGCAGTCTTC CTTGTTGGAT AGTCCTACCA AGCCACCCTT TGACCTCTCA 
GACACTATGG GCCACCCTCT GAAACTGGGC AGCATCTTCA CATTCAGAGT GACTGTCTTG 
CAGGCATCGA GTATACCTCC AGAATATGCT GACATATTCT GCCAATTCAA TTTTATTCAT 
CGCCATGATG AGGCCTTTTC AACTGAACCA TTAAAAAACA CAGGCCGAGG GCCACCACTG 
GGCTTCTACC ATGTGCAAAA TATTGCTGTG GAGGTCACTC AGTCATTTGT GGAATATGTG 
AAGAGTCAGC CAATTGTCTT TGAGGTGTTT GGACATTATC AGCAGCATCC TTTCCCTCCT 
CTGTGCAAAG ATGTTCTAAG CCCTCTGCGC TCTTCCAGAC GCCTGCTTCC AAGGGTCATG 
CCTCTGTCTA AACCAGTTCC TGCCACTAAG TTGAGCAGAC AGCGAGTTCC CAGTGTTGGT 
CCCTGTCACT GCAAGTATGA CCTGATGGCT TACTTCCAGA TCTGTGAACT TGAAGCCAAT 
GGAGATTACA TACCTGTGTC AGTAGATCAT GGAGTGGGTT TGCCTTGTCA TGGGACATTT 
CTTCTCCACC AGGGTATCCA GAGACGCATT TCAGTGACAC TGGTGCATGA AGCTGGCGCT 
GAGCTGAAGT GGCTGGAAGT GCGAGAGCTG GTTGTAGGTC GCATCCGGAA TACCCCAGAG 
GCAGATGAGT CTCTAATTGA CCCAAACATC TTGTCACTGA ATATTCTATC TGCGGGATAT 
CGGCCCCCTG AAGGAGGTGA TCGGACATTC TATCACTTTG AGGTGGCCTG GGACAGCTCT 
ATGCACAACT CTTTGTTGCT GAATCGTGTC ACTCCATACA GAGAGAAGAT CTACATGACA 
TTGTCTGCCT ATGTTGAGAT GGAAAATTGT ACACAGCCTG CTGTGATCAC CAAAGACTTC 
TGCATGGTTT TCTATTCTCG AGATGCCAAA CTCCCAGCTT GTCGCTCCAT TCGGAACCTG 
TTTGGAACTG GCAGCTTACG AGCCTCTGAA AGTAATCGTG TGACTGGAGT TTATGAGATG 
AGTCTGTGTC GTGTTGCAGA AACCAGTAGT CCAGGGATGC AGCGGAGACG GAGACGTGTG 
CTAGACACCT CAGTAGCATA TGTACGTGGG GAGGAAAACC TGGCAGGGTG GAGGCCACGG 
AGTGACAGCC TTATCCTTGA CCACCAGTGG GAACTGGAGA AACTTAGCTT GCTTCAGGAG 
GTGGAGAAAA CTCGCCATTA CCTGCTGCTG AGAGAGAAAC TGGAAGCCAC ACTCAGGGGA 
GCCACAGAAT CTCTCACCCC TTCCCCAATT GAGGAGATGC TGCCCCTTAG CCCAACATGT 
CAGCCGCTTC AGCTCACAGA GGTTGGATCA CCCCGTGCAG AAGAGAACCC CCTAGAAACT 
GCCAGCGAAA GGCAGAAAGA GCTGGCTGCC AAGTGTCTTC GCCTGCTCAC CCACACCTTT 
AACAGAGAGT ACAGTTACAG TCATGTATGT GTGAGTGCCA GCGAGAGTAA GCTTTCTGAA 
ATGTCAGTTA CACTTTTACG GGACCCCTCA CTGTCTGGCT TAGGGGTCAA CTCCCTTACA 
CCATCCAGCA CCTGCCCTTC CCTAGTAGAA GGACGTTACT GCAGTCCAGA GGCTAGCCCA 
CAACCTATCT CCCGCGTATT CAGCCCTGAG AGAGAGCTGC TGAGCCCAGC CGGAGACCTA 
CTGAAATCTC CCAGTGGTGA ACTGAAGACT CCCACTCTAA ACCTGTTTGT ACCAGAAATA 
CAAGAAGTCA GAGTTAGTCC AATTGTCTCT AAGAAGGGAT ACCTACACTT CCTGGAGCCA 
CATACAGGAG GCTGGGTGAA GAGATATGTA GTAGTCAGGC GACCCTATGT CTACATTTAC 
AATAGCGATC GGGACCCAGT GGAGAGAGCC ATCCTTAACC TCTCCCAGGC CCATGTTGAA 
TATAGTGAGG ACCAACAAGC TATGCTGAAG ACCCCCAACA CTTTTGCAGT ATGCACACAG 
CACCGCGGCA TCCTGCTGCA GGCCAGCAAT GACAAAGACA TGCACGACTG GCTCTACGCC 
TTTAACCCTC TCCTGGCGGG CTCCATCCGG TCCAAACTGT CCAGGCGCAG ATCAGCCCAA 
ATGAGAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949944.1
CDS	223..5514
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDTVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGSSPLFTTCMSESMAEITPSPTLSSPGSEGPEPASQLEEEDIFQDDEEEDDGPQEHPCCAVRSDPFHDRSPLFSVVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_018094455.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094455.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGCTCTC CCCTATTCAC CACATGCATG 
AGCGAGAGCA TGGCCGAAAT TACTCCCTCC CCCACTCTAT CGAGCCCCGG CTCCGAAGGC 
CCGGAGCCGG CCTCCCAGCT TGAAGAGGAG GATATTTTTC AGGATGATGA GGAAGAGGAT 
GATGGACCTC AGGAGCATCC CTGCTGTGCT GTCAGATCAG ACCCGTTTCA CGACCGATCC 
CCCCTGTTCA GTGTAGTGGG AAGGGCTTTT GTGTACCTGA GTAATTTGCT GTACTCTGTC 
CCTTTGGTTC ATCGAGTCGC CATTGTCAGT GAGAAAGGAG AAGTGAAAGG ATTTCTTCGT 
GTTGCTGTTC AGGCCATATC AGCTGACGAG GAAGCTCCTG ATTATGGTTC TGGAGTCCGG 
CAGTCTGGCA CAGCTAAAAT ATCCTTTGAT GATCAACATT TTCAAAAGTT CCAGGCTGAA 
ACCTGCCCAG GAATGACACG CTCAGGCACT TCACAAGAAG AGCTTCGAAT TGTTGAGGGA 
GGACAAGGAC AAAACCCGGA CTCGTGTCCT TCTGCTGACG AAGTGAACAA CAATACCTGT 
GCAGCTTCTC TGCAGTCTTC CTTGTTGGAT AGTCCTACCA AGCCACCCTT TGACCTCTCA 
GACACTATGG GCCACCCTCT GAAACTGGGC AGCATCTTCA CATTCAGAGT GACTGTCTTG 
CAGGCATCGA GTATACCTCC AGAATATGCT GACATATTCT GCCAATTCAA TTTTATTCAT 
CGCCATGATG AGGCCTTTTC AACTGAACCA TTAAAAAACA CAGGCCGAGG GCCACCACTG 
GGCTTCTACC ATGTGCAAAA TATTGCTGTG GAGGTCACTC AGTCATTTGT GGAATATGTG 
AAGAGTCAGC CAATTGTCTT TGAGGTGTTT GGACATTATC AGCAGCATCC TTTCCCTCCT 
CTGTGCAAAG ATGTTCTAAG CCCTCTGCGC TCTTCCAGAC GCCTGCTTCC AAGGGTCATG 
CCTCTGTCTA AACCAGTTCC TGCCACTAAG TTGAGCAGAC AGCGAGTTCC CAGTGTTGGT 
CCCTGTCACT GCAAGTATGA CCTGATGGCT TACTTCCAGA TCTGTGAACT TGAAGCCAAT 
GGAGATTACA TACCTGTGTC AGTAGATCAT GGAGTGGGTT TGCCTTGTCA TGGGACATTT 
CTTCTCCACC AGGGTATCCA GAGACGCATT TCAGTGACAC TGGTGCATGA AGCTGGCGCT 
GAGCTGAAGT GGCTGGAAGT GCGAGAGCTG GTTGTAGGTC GCATCCGGAA TACCCCAGAG 
GCAGATGAGT CTCTAATTGA CCCAAACATC TTGTCACTGA ATATTCTATC TGCGGGATAT 
CGGCCCCCTG AAGGAGGTGA TCGGACATTC TATCACTTTG AGGTGGCCTG GGACAGCTCT 
ATGCACAACT CTTTGTTGCT GAATCGTGTC ACTCCATACA GAGAGAAGAT CTACATGACA 
TTGTCTGCCT ATGTTGAGAT GGAAAATTGT ACACAGCCTG CTGTGATCAC CAAAGACTTC 
TGCATGGTTT TCTATTCTCG AGATGCCAAA CTCCCAGCTT GTCGCTCCAT TCGGAACCTG 
TTTGGAACTG GCAGCTTACG AGCCTCTGAA AGTAATCGTG TGACTGGAGT TTATGAGATG 
AGTCTGTGTC GTGTTGCAGA AACCAGTAGT CCAGGGATGC AGCGGAGACG GAGACGTGTG 
CTAGACACCT CAGTAGCATA TGTACGTGGG GAGGAAAACC TGGCAGGGTG GAGGCCACGG 
AGTGACAGCC TTATCCTTGA CCACCAGTGG GAACTGGAGA AACTTAGCTT GCTTCAGGAG 
GTGGAGAAAA CTCGCCATTA CCTGCTGCTG AGAGAGAAAC TGGAAGCCAC ACTCAGGGGA 
GCCACAGAAT CTCTCACCCC TTCCCCAATT GAGGAGATGC TGCCCCTTAG CCCAACATGT 
CAGCCGCTTC AGCTCACAGA GGTTGGATCA CCCCGTGCAG AAGAGAACCC CCTAGAAACT 
GCCAGCGAAA GGCAGAAAGA GCTGGCTGCC AAGTGTCTTC GCCTGCTCAC CCACACCTTT 
AACAGAGAGT ACAGTTACAG TCATGTATGT GTGAGTGCCA GCGAGAGTAA GCTTTCTGAA 
ATGTCAGTTA CACTTTTACG GGACCCCTCA CTGTCTGGCT TAGGGGTCAA CTCCCTTACA 
CCATCCAGCA CCTGCCCTTC CCTAGTAGAA GGACGTTACT GCAGTCCAGA GGCTAGCCCA 
CAACCTATCT CCCGCGTATT CAGCCCTGAG AGAGAGCTGC TGAGCCCAGC CGGAGACCTA 
CTGAAATCTC CCAGTGGTGA ACTGAAGACT CCCACTCTAA ACCTGTTTGT ACCAGAAATA 
CAAGAAGTCA GAGTTAGTCC AATTGTCTCT AAGAAGGGAT ACCTACACTT CCTGGAGCCA 
CATACAGGAG GCTGGGTGAA GAGATATGTA GTAGTCAGGC GACCCTATGT CTACATTTAC 
AATAGCGATC GGGACCCAGT GGAGAGAGCC ATCCTTAACC TCTCCCAGGC CCATGTTGAA 
TATAGTGAGG ACCAACAAGC TATGCTGAAG ACCCCCAACA CTTTTGCAGT ATGCACACAG 
CACCGCGGCA TCCTGCTGCA GGCCAGCAAT GACAAAGACA TGCACGACTG GCTCTACGCC 
TTTAACCCTC TCCTGGCGGG CTCCATCCGG TCCAAACTGT CCAGGCGCAG ATCAGCCCAA 
ATGAGAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_018094456.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949945.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X8, mRNA.

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CloneID	OXa44803
Clone ID Related Accession (Same CDS sequence)	XM_018094456.1
Accession Version	XM_018094456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG ACATTCTATC ACTTTGAGGT GGCCTGGGAC 
AGCTCTATGC ACAACTCTTT GTTGCTGAAT CGTGTCACTC CATACAGAGA GAAGATCTAC 
ATGACATTGT CTGCCTATGT TGAGATGGAA AATTGTACAC AGCCTGCTGT GATCACCAAA 
GACTTCTGCA TGGTTTTCTA TTCTCGAGAT GCCAAACTCC CAGCTTGTCG CTCCATTCGG 
AACCTGTTTG GAACTGGCAG CTTACGAGCC TCTGAAAGTA ATCGTGTGAC TGGAGTTTAT 
GAGATGAGTC TGTGTCGTGT TGCAGAAACC AGTAGTCCAG GGATGCAGCG GAGACGGAGA 
CGTGTGCTAG ACACCTCAGT AGCATATGTA CGTGGGGAGG AAAACCTGGC AGGGTGGAGG 
CCACGGAGTG ACAGCCTTAT CCTTGACCAC CAGTGGGAAC TGGAGAAACT TAGCTTGCTT 
CAGGAGGTGG AGAAAACTCG CCATTACCTG CTGCTGAGAG AGAAACTGGA AGCCACACTC 
AGGGGAGCCA CAGAATCTCT CACCCCTTCC CCAATTGAGG AGATGCTGCC CCTTAGCCCA 
ACATGTCAGC CGCTTCAGCT CACAGAGGTT GGATCACCCC GTGCAGAAGA GAACCCCCTA 
GAAACTGCCA GCGAAAGGCA GAAAGAGCTG GCTGCCAAGT GTCTTCGCCT GCTCACCCAC 
ACCTTTAACA GAGAGTACAG TTACAGTCAT GTATGTGTGA GTGCCAGCGA GAGTAAGCTT 
TCTGAAATGT CAGTTACACT TTTACGGGAC CCCTCACTGT CTGGCTTAGG GGTCAACTCC 
CTTACACCAT CCAGCACCTG CCCTTCCCTA GTAGAAGGAC GTTACTGCAG TCCAGAGGCT 
AGCCCACAAC CTATCTCCCG CGTATTCAGC CCTGAGAGAG AGCTGCTGAG CCCAGCCGGA 
GACCTACTGA AATCTCCCAG TGGTGAACTG AAGACTCCCA CTCTAAACCT GTTTGTACCA 
GAAATACAAG AAGTCAGAGT TAGTCCAATT GTCTCTAAGA AGGGATACCT ACACTTCCTG 
GAGCCACATA CAGGAGGCTG GGTGAAGAGA TATGTAGTAG TCAGGCGACC CTATGTCTAC 
ATTTACAATA GCGATCGGGA CCCAGTGGAG AGAGCCATCC TTAACCTCTC CCAGGCCCAT 
GTTGAATATA GTGAGGACCA ACAAGCTATG CTGAAGACCC CCAACACTTT TGCAGTATGC 
ACACAGCACC GCGGCATCCT GCTGCAGGCC AGCAATGACA AAGACATGCA CGACTGGCTC 
TACGCCTTTA ACCCTCTCCT GGCGGGCTCC ATCCGGTCCA AACTGTCCAG GCGCAGATCA 
GCCCAAATGA GAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949945.1
CDS	222..5279
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDTVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_018094456.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094456.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG ACATTCTATC ACTTTGAGGT GGCCTGGGAC 
AGCTCTATGC ACAACTCTTT GTTGCTGAAT CGTGTCACTC CATACAGAGA GAAGATCTAC 
ATGACATTGT CTGCCTATGT TGAGATGGAA AATTGTACAC AGCCTGCTGT GATCACCAAA 
GACTTCTGCA TGGTTTTCTA TTCTCGAGAT GCCAAACTCC CAGCTTGTCG CTCCATTCGG 
AACCTGTTTG GAACTGGCAG CTTACGAGCC TCTGAAAGTA ATCGTGTGAC TGGAGTTTAT 
GAGATGAGTC TGTGTCGTGT TGCAGAAACC AGTAGTCCAG GGATGCAGCG GAGACGGAGA 
CGTGTGCTAG ACACCTCAGT AGCATATGTA CGTGGGGAGG AAAACCTGGC AGGGTGGAGG 
CCACGGAGTG ACAGCCTTAT CCTTGACCAC CAGTGGGAAC TGGAGAAACT TAGCTTGCTT 
CAGGAGGTGG AGAAAACTCG CCATTACCTG CTGCTGAGAG AGAAACTGGA AGCCACACTC 
AGGGGAGCCA CAGAATCTCT CACCCCTTCC CCAATTGAGG AGATGCTGCC CCTTAGCCCA 
ACATGTCAGC CGCTTCAGCT CACAGAGGTT GGATCACCCC GTGCAGAAGA GAACCCCCTA 
GAAACTGCCA GCGAAAGGCA GAAAGAGCTG GCTGCCAAGT GTCTTCGCCT GCTCACCCAC 
ACCTTTAACA GAGAGTACAG TTACAGTCAT GTATGTGTGA GTGCCAGCGA GAGTAAGCTT 
TCTGAAATGT CAGTTACACT TTTACGGGAC CCCTCACTGT CTGGCTTAGG GGTCAACTCC 
CTTACACCAT CCAGCACCTG CCCTTCCCTA GTAGAAGGAC GTTACTGCAG TCCAGAGGCT 
AGCCCACAAC CTATCTCCCG CGTATTCAGC CCTGAGAGAG AGCTGCTGAG CCCAGCCGGA 
GACCTACTGA AATCTCCCAG TGGTGAACTG AAGACTCCCA CTCTAAACCT GTTTGTACCA 
GAAATACAAG AAGTCAGAGT TAGTCCAATT GTCTCTAAGA AGGGATACCT ACACTTCCTG 
GAGCCACATA CAGGAGGCTG GGTGAAGAGA TATGTAGTAG TCAGGCGACC CTATGTCTAC 
ATTTACAATA GCGATCGGGA CCCAGTGGAG AGAGCCATCC TTAACCTCTC CCAGGCCCAT 
GTTGAATATA GTGAGGACCA ACAAGCTATG CTGAAGACCC CCAACACTTT TGCAGTATGC 
ACACAGCACC GCGGCATCCT GCTGCAGGCC AGCAATGACA AAGACATGCA CGACTGGCTC 
TACGCCTTTA ACCCTCTCCT GGCGGGCTCC ATCCGGTCCA AACTGTCCAG GCGCAGATCA 
GCCCAAATGA GAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_031902551.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758411.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X1, mRNA.

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CloneID	OXa58871
Clone ID Related Accession (Same CDS sequence)	XM_031902551.1
Accession Version	XM_031902551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG CAGTTTCTTG ATTCTGATAC TCCTAGCTGC 
CAATCCGGGA GGGGCACCAG GACATTCTAT CACTTTGAGG TGGCCTGGGA CAGCTCTATG 
CACAACTCTT TGTTGCTGAA TCGTGTCACT CCATACAGAG AGAAGATCTA CATGACATTG 
TCTGCCTATG TTGAGATGGA AAATTGTACA CAGCCTGCTG TGATCACCAA AGACTTCTGC 
ATGGTTTTCT ATTCTCGAGA TGCCAAACTC CCAGCTTGTC GCTCCATTCG GAACCTGTTT 
GGAACTGGCA GCTTACGAGC CTCTGAAAGA TTCTCCTATA AGTTATTGCA TTACTGTACT 
CGGCTTACAT GCATGCTTAT GAATATGAGA GGCAAGGTGG GATATGCACC AAGTAATCGT 
GTGACTGGAG TTTATGAGAT GAGTCTGTGT CGTGTTGCAG AAACCAGTAG TCCAGGGATG 
CAGCGGAGAC GGAGACGTGT GCTAGACACC TCAGTAGCAT ATGTACGTGG GGAGGAAAAC 
CTGGCAGGGT GGAGGCCACG GAGTGACAGC CTTATCCTTG ACCACCAGTG GGAACTGGAG 
AAACTTAGCT TGCTTCAGGA GGTGGAGAAA ACTCGCCATT ACCTGCTGCT GAGAGAGAAA 
CTGGAAGCCA CACTCAGGGG AGCCACAGAA TCTCTCACCC CTTCCCCAAT TGAGGAGATG 
CTGCCCCTTA GCCCAACATG TCAGCCGCTT CAGCTCACAG AGGTTGGATC ACCCCGTGCA 
GAAGAGAACC CCCTAGAAAC TGCCAGCGAA AGGCAGAAAG AGCTGGCTGC CAAGTGTCTT 
CGCCTGCTCA CCCACACCTT TAACAGAGAG TACAGTTACA GTCATGTATG TGTGAGTGCC 
AGCGAGAGTA AGCTTTCTGA AATGTCAGTT ACACTTTTAC GGGACCCCTC ACTGTCTGGC 
TTAGGGGTCA ACTCCCTTAC ACCATCCAGC ACCTGCCCTT CCCTAGTAGA AGGACGTTAC 
TGCAGTCCAG AGGCTAGCCC ACAACCTATC TCCCGCGTAT TCAGCCCTGA GAGAGAGCTG 
CTGAGCCCAG CCGGAGACCT ACTGAAATCT CCCAGTGGTG AACTGAAGAC TCCCACTCTA 
AACCTGTTTG TACCAGAAAT ACAAGAAGTC AGAGTTAGTC CAATTGTCTC TAAGAAGGGA 
TACCTACACT TCCTGGAGCC ACATACAGGA GGCTGGGTGA AGAGATATGT AGTAGTCAGG 
CGACCCTATG TCTACATTTA CAATAGCGAT CGGGACCCAG TGGAGAGAGC CATCCTTAAC 
CTCTCCCAGG CCCATGTTGA ATATAGTGAG GACCAACAAG CTATGCTGAA GACCCCCAAC 
ACTTTTGCAG TATGCACACA GCACCGCGGC ATCCTGCTGC AGGCCAGCAA TGACAAAGAC 
ATGCACGACT GGCTCTACGC CTTTAACCCT CTCCTGGCGG GCTCCATCCG GTCCAAACTG 
TCCAGGCGCA GATCAGCCCA AATGAGAATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758411.1
CDS	217..5709
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGSSPLFTTCMSESMAEITPSPTLSSPGSEGPEPASQLEEEDIFQDDEEEDDGPQEHPCCAVRSDPFHDRSPLFSVVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRQFLDSDTPSCQSGRGTRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASERFSYKLLHYCTRLTCMLMNMRGKVGYAPSNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_031902551.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902551.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG CAGTTTCTTG ATTCTGATAC TCCTAGCTGC 
CAATCCGGGA GGGGCACCAG GACATTCTAT CACTTTGAGG TGGCCTGGGA CAGCTCTATG 
CACAACTCTT TGTTGCTGAA TCGTGTCACT CCATACAGAG AGAAGATCTA CATGACATTG 
TCTGCCTATG TTGAGATGGA AAATTGTACA CAGCCTGCTG TGATCACCAA AGACTTCTGC 
ATGGTTTTCT ATTCTCGAGA TGCCAAACTC CCAGCTTGTC GCTCCATTCG GAACCTGTTT 
GGAACTGGCA GCTTACGAGC CTCTGAAAGA TTCTCCTATA AGTTATTGCA TTACTGTACT 
CGGCTTACAT GCATGCTTAT GAATATGAGA GGCAAGGTGG GATATGCACC AAGTAATCGT 
GTGACTGGAG TTTATGAGAT GAGTCTGTGT CGTGTTGCAG AAACCAGTAG TCCAGGGATG 
CAGCGGAGAC GGAGACGTGT GCTAGACACC TCAGTAGCAT ATGTACGTGG GGAGGAAAAC 
CTGGCAGGGT GGAGGCCACG GAGTGACAGC CTTATCCTTG ACCACCAGTG GGAACTGGAG 
AAACTTAGCT TGCTTCAGGA GGTGGAGAAA ACTCGCCATT ACCTGCTGCT GAGAGAGAAA 
CTGGAAGCCA CACTCAGGGG AGCCACAGAA TCTCTCACCC CTTCCCCAAT TGAGGAGATG 
CTGCCCCTTA GCCCAACATG TCAGCCGCTT CAGCTCACAG AGGTTGGATC ACCCCGTGCA 
GAAGAGAACC CCCTAGAAAC TGCCAGCGAA AGGCAGAAAG AGCTGGCTGC CAAGTGTCTT 
CGCCTGCTCA CCCACACCTT TAACAGAGAG TACAGTTACA GTCATGTATG TGTGAGTGCC 
AGCGAGAGTA AGCTTTCTGA AATGTCAGTT ACACTTTTAC GGGACCCCTC ACTGTCTGGC 
TTAGGGGTCA ACTCCCTTAC ACCATCCAGC ACCTGCCCTT CCCTAGTAGA AGGACGTTAC 
TGCAGTCCAG AGGCTAGCCC ACAACCTATC TCCCGCGTAT TCAGCCCTGA GAGAGAGCTG 
CTGAGCCCAG CCGGAGACCT ACTGAAATCT CCCAGTGGTG AACTGAAGAC TCCCACTCTA 
AACCTGTTTG TACCAGAAAT ACAAGAAGTC AGAGTTAGTC CAATTGTCTC TAAGAAGGGA 
TACCTACACT TCCTGGAGCC ACATACAGGA GGCTGGGTGA AGAGATATGT AGTAGTCAGG 
CGACCCTATG TCTACATTTA CAATAGCGAT CGGGACCCAG TGGAGAGAGC CATCCTTAAC 
CTCTCCCAGG CCCATGTTGA ATATAGTGAG GACCAACAAG CTATGCTGAA GACCCCCAAC 
ACTTTTGCAG TATGCACACA GCACCGCGGC ATCCTGCTGC AGGCCAGCAA TGACAAAGAC 
ATGCACGACT GGCTCTACGC CTTTAACCCT CTCCTGGCGG GCTCCATCCG GTCCAAACTG 
TCCAGGCGCA GATCAGCCCA AATGAGAATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_031902552.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758412.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X2, mRNA.

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CloneID	OXa58872
Clone ID Related Accession (Same CDS sequence)	XM_031902552.1
Accession Version	XM_031902552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG CAGTTTCTTG ATTCTGATAC TCCTAGGACA 
TTCTATCACT TTGAGGTGGC CTGGGACAGC TCTATGCACA ACTCTTTGTT GCTGAATCGT 
GTCACTCCAT ACAGAGAGAA GATCTACATG ACATTGTCTG CCTATGTTGA GATGGAAAAT 
TGTACACAGC CTGCTGTGAT CACCAAAGAC TTCTGCATGG TTTTCTATTC TCGAGATGCC 
AAACTCCCAG CTTGTCGCTC CATTCGGAAC CTGTTTGGAA CTGGCAGCTT ACGAGCCTCT 
GAAAGATTCT CCTATAAGTT ATTGCATTAC TGTACTCGGC TTACATGCAT GCTTATGAAT 
ATGAGAGGCA AGGTGGGATA TGCACCAAGT AATCGTGTGA CTGGAGTTTA TGAGATGAGT 
CTGTGTCGTG TTGCAGAAAC CAGTAGTCCA GGGATGCAGC GGAGACGGAG ACGTGTGCTA 
GACACCTCAG TAGCATATGT ACGTGGGGAG GAAAACCTGG CAGGGTGGAG GCCACGGAGT 
GACAGCCTTA TCCTTGACCA CCAGTGGGAA CTGGAGAAAC TTAGCTTGCT TCAGGAGGTG 
GAGAAAACTC GCCATTACCT GCTGCTGAGA GAGAAACTGG AAGCCACACT CAGGGGAGCC 
ACAGAATCTC TCACCCCTTC CCCAATTGAG GAGATGCTGC CCCTTAGCCC AACATGTCAG 
CCGCTTCAGC TCACAGAGGT TGGATCACCC CGTGCAGAAG AGAACCCCCT AGAAACTGCC 
AGCGAAAGGC AGAAAGAGCT GGCTGCCAAG TGTCTTCGCC TGCTCACCCA CACCTTTAAC 
AGAGAGTACA GTTACAGTCA TGTATGTGTG AGTGCCAGCG AGAGTAAGCT TTCTGAAATG 
TCAGTTACAC TTTTACGGGA CCCCTCACTG TCTGGCTTAG GGGTCAACTC CCTTACACCA 
TCCAGCACCT GCCCTTCCCT AGTAGAAGGA CGTTACTGCA GTCCAGAGGC TAGCCCACAA 
CCTATCTCCC GCGTATTCAG CCCTGAGAGA GAGCTGCTGA GCCCAGCCGG AGACCTACTG 
AAATCTCCCA GTGGTGAACT GAAGACTCCC ACTCTAAACC TGTTTGTACC AGAAATACAA 
GAAGTCAGAG TTAGTCCAAT TGTCTCTAAG AAGGGATACC TACACTTCCT GGAGCCACAT 
ACAGGAGGCT GGGTGAAGAG ATATGTAGTA GTCAGGCGAC CCTATGTCTA CATTTACAAT 
AGCGATCGGG ACCCAGTGGA GAGAGCCATC CTTAACCTCT CCCAGGCCCA TGTTGAATAT 
AGTGAGGACC AACAAGCTAT GCTGAAGACC CCCAACACTT TTGCAGTATG CACACAGCAC 
CGCGGCATCC TGCTGCAGGC CAGCAATGAC AAAGACATGC ACGACTGGCT CTACGCCTTT 
AACCCTCTCC TGGCGGGCTC CATCCGGTCC AAACTGTCCA GGCGCAGATC AGCCCAAATG 
AGAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758412.1
CDS	217..5685
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGSSPLFTTCMSESMAEITPSPTLSSPGSEGPEPASQLEEEDIFQDDEEEDDGPQEHPCCAVRSDPFHDRSPLFSVVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRQFLDSDTPRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASERFSYKLLHYCTRLTCMLMNMRGKVGYAPSNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_031902552.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902552.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG CAGTTTCTTG ATTCTGATAC TCCTAGGACA 
TTCTATCACT TTGAGGTGGC CTGGGACAGC TCTATGCACA ACTCTTTGTT GCTGAATCGT 
GTCACTCCAT ACAGAGAGAA GATCTACATG ACATTGTCTG CCTATGTTGA GATGGAAAAT 
TGTACACAGC CTGCTGTGAT CACCAAAGAC TTCTGCATGG TTTTCTATTC TCGAGATGCC 
AAACTCCCAG CTTGTCGCTC CATTCGGAAC CTGTTTGGAA CTGGCAGCTT ACGAGCCTCT 
GAAAGATTCT CCTATAAGTT ATTGCATTAC TGTACTCGGC TTACATGCAT GCTTATGAAT 
ATGAGAGGCA AGGTGGGATA TGCACCAAGT AATCGTGTGA CTGGAGTTTA TGAGATGAGT 
CTGTGTCGTG TTGCAGAAAC CAGTAGTCCA GGGATGCAGC GGAGACGGAG ACGTGTGCTA 
GACACCTCAG TAGCATATGT ACGTGGGGAG GAAAACCTGG CAGGGTGGAG GCCACGGAGT 
GACAGCCTTA TCCTTGACCA CCAGTGGGAA CTGGAGAAAC TTAGCTTGCT TCAGGAGGTG 
GAGAAAACTC GCCATTACCT GCTGCTGAGA GAGAAACTGG AAGCCACACT CAGGGGAGCC 
ACAGAATCTC TCACCCCTTC CCCAATTGAG GAGATGCTGC CCCTTAGCCC AACATGTCAG 
CCGCTTCAGC TCACAGAGGT TGGATCACCC CGTGCAGAAG AGAACCCCCT AGAAACTGCC 
AGCGAAAGGC AGAAAGAGCT GGCTGCCAAG TGTCTTCGCC TGCTCACCCA CACCTTTAAC 
AGAGAGTACA GTTACAGTCA TGTATGTGTG AGTGCCAGCG AGAGTAAGCT TTCTGAAATG 
TCAGTTACAC TTTTACGGGA CCCCTCACTG TCTGGCTTAG GGGTCAACTC CCTTACACCA 
TCCAGCACCT GCCCTTCCCT AGTAGAAGGA CGTTACTGCA GTCCAGAGGC TAGCCCACAA 
CCTATCTCCC GCGTATTCAG CCCTGAGAGA GAGCTGCTGA GCCCAGCCGG AGACCTACTG 
AAATCTCCCA GTGGTGAACT GAAGACTCCC ACTCTAAACC TGTTTGTACC AGAAATACAA 
GAAGTCAGAG TTAGTCCAAT TGTCTCTAAG AAGGGATACC TACACTTCCT GGAGCCACAT 
ACAGGAGGCT GGGTGAAGAG ATATGTAGTA GTCAGGCGAC CCTATGTCTA CATTTACAAT 
AGCGATCGGG ACCCAGTGGA GAGAGCCATC CTTAACCTCT CCCAGGCCCA TGTTGAATAT 
AGTGAGGACC AACAAGCTAT GCTGAAGACC CCCAACACTT TTGCAGTATG CACACAGCAC 
CGCGGCATCC TGCTGCAGGC CAGCAATGAC AAAGACATGC ACGACTGGCT CTACGCCTTT 
AACCCTCTCC TGGCGGGCTC CATCCGGTCC AAACTGTCCA GGCGCAGATC AGCCCAAATG 
AGAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_031902553.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758413.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X3, mRNA.

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CloneID	OXa58873
Clone ID Related Accession (Same CDS sequence)	XM_031902553.1
Accession Version	XM_031902553.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGC TGCCAATCCG GGAGGGGCAC CAGGACATTC 
TATCACTTTG AGGTGGCCTG GGACAGCTCT ATGCACAACT CTTTGTTGCT GAATCGTGTC 
ACTCCATACA GAGAGAAGAT CTACATGACA TTGTCTGCCT ATGTTGAGAT GGAAAATTGT 
ACACAGCCTG CTGTGATCAC CAAAGACTTC TGCATGGTTT TCTATTCTCG AGATGCCAAA 
CTCCCAGCTT GTCGCTCCAT TCGGAACCTG TTTGGAACTG GCAGCTTACG AGCCTCTGAA 
AGATTCTCCT ATAAGTTATT GCATTACTGT ACTCGGCTTA CATGCATGCT TATGAATATG 
AGAGGCAAGG TGGGATATGC ACCAAGTAAT CGTGTGACTG GAGTTTATGA GATGAGTCTG 
TGTCGTGTTG CAGAAACCAG TAGTCCAGGG ATGCAGCGGA GACGGAGACG TGTGCTAGAC 
ACCTCAGTAG CATATGTACG TGGGGAGGAA AACCTGGCAG GGTGGAGGCC ACGGAGTGAC 
AGCCTTATCC TTGACCACCA GTGGGAACTG GAGAAACTTA GCTTGCTTCA GGAGGTGGAG 
AAAACTCGCC ATTACCTGCT GCTGAGAGAG AAACTGGAAG CCACACTCAG GGGAGCCACA 
GAATCTCTCA CCCCTTCCCC AATTGAGGAG ATGCTGCCCC TTAGCCCAAC ATGTCAGCCG 
CTTCAGCTCA CAGAGGTTGG ATCACCCCGT GCAGAAGAGA ACCCCCTAGA AACTGCCAGC 
GAAAGGCAGA AAGAGCTGGC TGCCAAGTGT CTTCGCCTGC TCACCCACAC CTTTAACAGA 
GAGTACAGTT ACAGTCATGT ATGTGTGAGT GCCAGCGAGA GTAAGCTTTC TGAAATGTCA 
GTTACACTTT TACGGGACCC CTCACTGTCT GGCTTAGGGG TCAACTCCCT TACACCATCC 
AGCACCTGCC CTTCCCTAGT AGAAGGACGT TACTGCAGTC CAGAGGCTAG CCCACAACCT 
ATCTCCCGCG TATTCAGCCC TGAGAGAGAG CTGCTGAGCC CAGCCGGAGA CCTACTGAAA 
TCTCCCAGTG GTGAACTGAA GACTCCCACT CTAAACCTGT TTGTACCAGA AATACAAGAA 
GTCAGAGTTA GTCCAATTGT CTCTAAGAAG GGATACCTAC ACTTCCTGGA GCCACATACA 
GGAGGCTGGG TGAAGAGATA TGTAGTAGTC AGGCGACCCT ATGTCTACAT TTACAATAGC 
GATCGGGACC CAGTGGAGAG AGCCATCCTT AACCTCTCCC AGGCCCATGT TGAATATAGT 
GAGGACCAAC AAGCTATGCT GAAGACCCCC AACACTTTTG CAGTATGCAC ACAGCACCGC 
GGCATCCTGC TGCAGGCCAG CAATGACAAA GACATGCACG ACTGGCTCTA CGCCTTTAAC 
CCTCTCCTGG CGGGCTCCAT CCGGTCCAAA CTGTCCAGGC GCAGATCAGC CCAAATGAGA 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758413.1
CDS	217..5682
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGSSPLFTTCMSESMAEITPSPTLSSPGSEGPEPASQLEEEDIFQDDEEEDDGPQEHPCCAVRSDPFHDRSPLFSVVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRCQSGRGTRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASERFSYKLLHYCTRLTCMLMNMRGKVGYAPSNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_031902553.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902553.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGC TGCCAATCCG GGAGGGGCAC CAGGACATTC 
TATCACTTTG AGGTGGCCTG GGACAGCTCT ATGCACAACT CTTTGTTGCT GAATCGTGTC 
ACTCCATACA GAGAGAAGAT CTACATGACA TTGTCTGCCT ATGTTGAGAT GGAAAATTGT 
ACACAGCCTG CTGTGATCAC CAAAGACTTC TGCATGGTTT TCTATTCTCG AGATGCCAAA 
CTCCCAGCTT GTCGCTCCAT TCGGAACCTG TTTGGAACTG GCAGCTTACG AGCCTCTGAA 
AGATTCTCCT ATAAGTTATT GCATTACTGT ACTCGGCTTA CATGCATGCT TATGAATATG 
AGAGGCAAGG TGGGATATGC ACCAAGTAAT CGTGTGACTG GAGTTTATGA GATGAGTCTG 
TGTCGTGTTG CAGAAACCAG TAGTCCAGGG ATGCAGCGGA GACGGAGACG TGTGCTAGAC 
ACCTCAGTAG CATATGTACG TGGGGAGGAA AACCTGGCAG GGTGGAGGCC ACGGAGTGAC 
AGCCTTATCC TTGACCACCA GTGGGAACTG GAGAAACTTA GCTTGCTTCA GGAGGTGGAG 
AAAACTCGCC ATTACCTGCT GCTGAGAGAG AAACTGGAAG CCACACTCAG GGGAGCCACA 
GAATCTCTCA CCCCTTCCCC AATTGAGGAG ATGCTGCCCC TTAGCCCAAC ATGTCAGCCG 
CTTCAGCTCA CAGAGGTTGG ATCACCCCGT GCAGAAGAGA ACCCCCTAGA AACTGCCAGC 
GAAAGGCAGA AAGAGCTGGC TGCCAAGTGT CTTCGCCTGC TCACCCACAC CTTTAACAGA 
GAGTACAGTT ACAGTCATGT ATGTGTGAGT GCCAGCGAGA GTAAGCTTTC TGAAATGTCA 
GTTACACTTT TACGGGACCC CTCACTGTCT GGCTTAGGGG TCAACTCCCT TACACCATCC 
AGCACCTGCC CTTCCCTAGT AGAAGGACGT TACTGCAGTC CAGAGGCTAG CCCACAACCT 
ATCTCCCGCG TATTCAGCCC TGAGAGAGAG CTGCTGAGCC CAGCCGGAGA CCTACTGAAA 
TCTCCCAGTG GTGAACTGAA GACTCCCACT CTAAACCTGT TTGTACCAGA AATACAAGAA 
GTCAGAGTTA GTCCAATTGT CTCTAAGAAG GGATACCTAC ACTTCCTGGA GCCACATACA 
GGAGGCTGGG TGAAGAGATA TGTAGTAGTC AGGCGACCCT ATGTCTACAT TTACAATAGC 
GATCGGGACC CAGTGGAGAG AGCCATCCTT AACCTCTCCC AGGCCCATGT TGAATATAGT 
GAGGACCAAC AAGCTATGCT GAAGACCCCC AACACTTTTG CAGTATGCAC ACAGCACCGC 
GGCATCCTGC TGCAGGCCAG CAATGACAAA GACATGCACG ACTGGCTCTA CGCCTTTAAC 
CCTCTCCTGG CGGGCTCCAT CCGGTCCAAA CTGTCCAGGC GCAGATCAGC CCAAATGAGA 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_031902554.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758414.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X4, mRNA.

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CloneID	OXa58874
Clone ID Related Accession (Same CDS sequence)	XM_031902554.1
Accession Version	XM_031902554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG ACATTCTATC ACTTTGAGGT GGCCTGGGAC 
AGCTCTATGC ACAACTCTTT GTTGCTGAAT CGTGTCACTC CATACAGAGA GAAGATCTAC 
ATGACATTGT CTGCCTATGT TGAGATGGAA AATTGTACAC AGCCTGCTGT GATCACCAAA 
GACTTCTGCA TGGTTTTCTA TTCTCGAGAT GCCAAACTCC CAGCTTGTCG CTCCATTCGG 
AACCTGTTTG GAACTGGCAG CTTACGAGCC TCTGAAAGAT TCTCCTATAA GTTATTGCAT 
TACTGTACTC GGCTTACATG CATGCTTATG AATATGAGAG GCAAGGTGGG ATATGCACCA 
AGTAATCGTG TGACTGGAGT TTATGAGATG AGTCTGTGTC GTGTTGCAGA AACCAGTAGT 
CCAGGGATGC AGCGGAGACG GAGACGTGTG CTAGACACCT CAGTAGCATA TGTACGTGGG 
GAGGAAAACC TGGCAGGGTG GAGGCCACGG AGTGACAGCC TTATCCTTGA CCACCAGTGG 
GAACTGGAGA AACTTAGCTT GCTTCAGGAG GTGGAGAAAA CTCGCCATTA CCTGCTGCTG 
AGAGAGAAAC TGGAAGCCAC ACTCAGGGGA GCCACAGAAT CTCTCACCCC TTCCCCAATT 
GAGGAGATGC TGCCCCTTAG CCCAACATGT CAGCCGCTTC AGCTCACAGA GGTTGGATCA 
CCCCGTGCAG AAGAGAACCC CCTAGAAACT GCCAGCGAAA GGCAGAAAGA GCTGGCTGCC 
AAGTGTCTTC GCCTGCTCAC CCACACCTTT AACAGAGAGT ACAGTTACAG TCATGTATGT 
GTGAGTGCCA GCGAGAGTAA GCTTTCTGAA ATGTCAGTTA CACTTTTACG GGACCCCTCA 
CTGTCTGGCT TAGGGGTCAA CTCCCTTACA CCATCCAGCA CCTGCCCTTC CCTAGTAGAA 
GGACGTTACT GCAGTCCAGA GGCTAGCCCA CAACCTATCT CCCGCGTATT CAGCCCTGAG 
AGAGAGCTGC TGAGCCCAGC CGGAGACCTA CTGAAATCTC CCAGTGGTGA ACTGAAGACT 
CCCACTCTAA ACCTGTTTGT ACCAGAAATA CAAGAAGTCA GAGTTAGTCC AATTGTCTCT 
AAGAAGGGAT ACCTACACTT CCTGGAGCCA CATACAGGAG GCTGGGTGAA GAGATATGTA 
GTAGTCAGGC GACCCTATGT CTACATTTAC AATAGCGATC GGGACCCAGT GGAGAGAGCC 
ATCCTTAACC TCTCCCAGGC CCATGTTGAA TATAGTGAGG ACCAACAAGC TATGCTGAAG 
ACCCCCAACA CTTTTGCAGT ATGCACACAG CACCGCGGCA TCCTGCTGCA GGCCAGCAAT 
GACAAAGACA TGCACGACTG GCTCTACGCC TTTAACCCTC TCCTGGCGGG CTCCATCCGG 
TCCAAACTGT CCAGGCGCAG ATCAGCCCAA ATGAGAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758414.1
CDS	217..5658
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGSSPLFTTCMSESMAEITPSPTLSSPGSEGPEPASQLEEEDIFQDDEEEDDGPQEHPCCAVRSDPFHDRSPLFSVVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASERFSYKLLHYCTRLTCMLMNMRGKVGYAPSNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_031902554.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902554.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG ACATTCTATC ACTTTGAGGT GGCCTGGGAC 
AGCTCTATGC ACAACTCTTT GTTGCTGAAT CGTGTCACTC CATACAGAGA GAAGATCTAC 
ATGACATTGT CTGCCTATGT TGAGATGGAA AATTGTACAC AGCCTGCTGT GATCACCAAA 
GACTTCTGCA TGGTTTTCTA TTCTCGAGAT GCCAAACTCC CAGCTTGTCG CTCCATTCGG 
AACCTGTTTG GAACTGGCAG CTTACGAGCC TCTGAAAGAT TCTCCTATAA GTTATTGCAT 
TACTGTACTC GGCTTACATG CATGCTTATG AATATGAGAG GCAAGGTGGG ATATGCACCA 
AGTAATCGTG TGACTGGAGT TTATGAGATG AGTCTGTGTC GTGTTGCAGA AACCAGTAGT 
CCAGGGATGC AGCGGAGACG GAGACGTGTG CTAGACACCT CAGTAGCATA TGTACGTGGG 
GAGGAAAACC TGGCAGGGTG GAGGCCACGG AGTGACAGCC TTATCCTTGA CCACCAGTGG 
GAACTGGAGA AACTTAGCTT GCTTCAGGAG GTGGAGAAAA CTCGCCATTA CCTGCTGCTG 
AGAGAGAAAC TGGAAGCCAC ACTCAGGGGA GCCACAGAAT CTCTCACCCC TTCCCCAATT 
GAGGAGATGC TGCCCCTTAG CCCAACATGT CAGCCGCTTC AGCTCACAGA GGTTGGATCA 
CCCCGTGCAG AAGAGAACCC CCTAGAAACT GCCAGCGAAA GGCAGAAAGA GCTGGCTGCC 
AAGTGTCTTC GCCTGCTCAC CCACACCTTT AACAGAGAGT ACAGTTACAG TCATGTATGT 
GTGAGTGCCA GCGAGAGTAA GCTTTCTGAA ATGTCAGTTA CACTTTTACG GGACCCCTCA 
CTGTCTGGCT TAGGGGTCAA CTCCCTTACA CCATCCAGCA CCTGCCCTTC CCTAGTAGAA 
GGACGTTACT GCAGTCCAGA GGCTAGCCCA CAACCTATCT CCCGCGTATT CAGCCCTGAG 
AGAGAGCTGC TGAGCCCAGC CGGAGACCTA CTGAAATCTC CCAGTGGTGA ACTGAAGACT 
CCCACTCTAA ACCTGTTTGT ACCAGAAATA CAAGAAGTCA GAGTTAGTCC AATTGTCTCT 
AAGAAGGGAT ACCTACACTT CCTGGAGCCA CATACAGGAG GCTGGGTGAA GAGATATGTA 
GTAGTCAGGC GACCCTATGT CTACATTTAC AATAGCGATC GGGACCCAGT GGAGAGAGCC 
ATCCTTAACC TCTCCCAGGC CCATGTTGAA TATAGTGAGG ACCAACAAGC TATGCTGAAG 
ACCCCCAACA CTTTTGCAGT ATGCACACAG CACCGCGGCA TCCTGCTGCA GGCCAGCAAT 
GACAAAGACA TGCACGACTG GCTCTACGCC TTTAACCCTC TCCTGGCGGG CTCCATCCGG 
TCCAAACTGT CCAGGCGCAG ATCAGCCCAA ATGAGAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_031902555.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758415.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X5, mRNA.

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CloneID	OXa58875
Clone ID Related Accession (Same CDS sequence)	XM_031902555.1
Accession Version	XM_031902555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGCTCTC CCCTATTCAC CACATGCATG 
AGCGAGAGCA TGGCCGAAAT TACTCCCTCC CCCACTCTAT CGAGCCCCGG CTCCGAAGGC 
CCGGAGCCGG CCTCCCAGCT TGAAGAGGAG GATATTTTTC AGGATGATGA GGAAGAGGAT 
GATGGACCTC AGGAGCATCC CTGCTGTGCT GTCAGATCAG ACCCGTTTCA CGACCGATCC 
CCCCTGTTCA GTGTAGTGGG AAGGGCTTTT GTGTACCTGA GTAATTTGCT GTACTCTGTC 
CCTTTGGTTC ATCGAGTCGC CATTGTCAGT GAGAAAGGAG AAGTGAAAGG ATTTCTTCGT 
GTTGCTGTTC AGGCCATATC AGCTGACGAG GAAGCTCCTG ATTATGGTTC TGGAGTCCGG 
CAGTCTGGCA CAGCTAAAAT ATCCTTTGAT GATCAACATT TTCAAAAGTT CCAGGCTGAA 
ACCTGCCCAG GAATGACACG CTCAGGCACT TCACAAGAAG AGCTTCGAAT TGTTGAGGGA 
GGACAAGGAC AAAACCCGGA CTCGTGTCCT TCTGCTGACG AAGTGAACAA CAATACCTGT 
GCAGCTTCTC TGCAGTCTTC CTTGTTGGAT AGTCCTACCA AGCCACCCTT TGACCTCTCA 
GACACTATGG GCCACCCTCT GAAACTGGGC AGCATCTTCA CATTCAGAGT GACTGTCTTG 
CAGGCATCGA GTATACCTCC AGAATATGCT GACATATTCT GCCAATTCAA TTTTATTCAT 
CGCCATGATG AGGCCTTTTC AACTGAACCA TTAAAAAACA CAGGCCGAGG GCCACCACTG 
GGCTTCTACC ATGTGCAAAA TATTGCTGTG GAGGTCACTC AGTCATTTGT GGAATATGTG 
AAGAGTCAGC CAATTGTCTT TGAGGTGTTT GGACATTATC AGCAGCATCC TTTCCCTCCT 
CTGTGCAAAG ATGTTCTAAG CCCTCTGCGC TCTTCCAGAC GCCTGCTTCC AAGGGTCATG 
CCTCTGTCTA AACCAGTTCC TGCCACTAAG TTGAGCAGAC AGCGAGTTCC CAGTGTTGGT 
CCCTGTCACT GCAAGTATGA CCTGATGGCT TACTTCCAGA TCTGTGAACT TGAAGCCAAT 
GGAGATTACA TACCTGTGTC AGTAGATCAT GGAGTGGGTT TGCCTTGTCA TGGGACATTT 
CTTCTCCACC AGGGTATCCA GAGACGCATT TCAGTGACAC TGGTGCATGA AGCTGGCGCT 
GAGCTGAAGT GGCTGGAAGT GCGAGAGCTG GTTGTAGGTC GCATCCGGAA TACCCCAGAG 
GCAGATGAGT CTCTAATTGA CCCAAACATC TTGTCACTGA ATATTCTATC TGCGGGATAT 
CGGCCCCCTG AAGGAGGTGA TCGGCAGTTT CTTGATTCTG ATACTCCTAG CTGCCAATCC 
GGGAGGGGCA CCAGGACATT CTATCACTTT GAGGTGGCCT GGGACAGCTC TATGCACAAC 
TCTTTGTTGC TGAATCGTGT CACTCCATAC AGAGAGAAGA TCTACATGAC ATTGTCTGCC 
TATGTTGAGA TGGAAAATTG TACACAGCCT GCTGTGATCA CCAAAGACTT CTGCATGGTT 
TTCTATTCTC GAGATGCCAA ACTCCCAGCT TGTCGCTCCA TTCGGAACCT GTTTGGAACT 
GGCAGCTTAC GAGCCTCTGA AAGATTCTCC TATAAGTTAT TGCATTACTG TACTCGGCTT 
ACATGCATGC TTATGAATAT GAGAGGCAAG GTGGGATATG CACCAAGTAA TCGTGTGACT 
GGAGTTTATG AGATGAGTCT GTGTCGTGTT GCAGAAACCA GTAGTCCAGG GATGCAGCGG 
AGACGGAGAC GTGTGCTAGA CACCTCAGTA GCATATGTAC GTGGGGAGGA AAACCTGGCA 
GGGTGGAGGC CACGGAGTGA CAGCCTTATC CTTGACCACC AGTGGGAACT GGAGAAACTT 
AGCTTGCTTC AGGAGGTGGA GAAAACTCGC CATTACCTGC TGCTGAGAGA GAAACTGGAA 
GCCACACTCA GGGGAGCCAC AGAATCTCTC ACCCCTTCCC CAATTGAGGA GATGCTGCCC 
CTTAGCCCAA CATGTCAGCC GCTTCAGCTC ACAGAGGTTG GATCACCCCG TGCAGAAGAG 
AACCCCCTAG AAACTGCCAG CGAAAGGCAG AAAGAGCTGG CTGCCAAGTG TCTTCGCCTG 
CTCACCCACA CCTTTAACAG AGAGTACAGT TACAGTCATG TATGTGTGAG TGCCAGCGAG 
AGTAAGCTTT CTGAAATGTC AGTTACACTT TTACGGGACC CCTCACTGTC TGGCTTAGGG 
GTCAACTCCC TTACACCATC CAGCACCTGC CCTTCCCTAG TAGAAGGACG TTACTGCAGT 
CCAGAGGCTA GCCCACAACC TATCTCCCGC GTATTCAGCC CTGAGAGAGA GCTGCTGAGC 
CCAGCCGGAG ACCTACTGAA ATCTCCCAGT GGTGAACTGA AGACTCCCAC TCTAAACCTG 
TTTGTACCAG AAATACAAGA AGTCAGAGTT AGTCCAATTG TCTCTAAGAA GGGATACCTA 
CACTTCCTGG AGCCACATAC AGGAGGCTGG GTGAAGAGAT ATGTAGTAGT CAGGCGACCC 
TATGTCTACA TTTACAATAG CGATCGGGAC CCAGTGGAGA GAGCCATCCT TAACCTCTCC 
CAGGCCCATG TTGAATATAG TGAGGACCAA CAAGCTATGC TGAAGACCCC CAACACTTTT 
GCAGTATGCA CACAGCACCG CGGCATCCTG CTGCAGGCCA GCAATGACAA AGACATGCAC 
GACTGGCTCT ACGCCTTTAA CCCTCTCCTG GCGGGCTCCA TCCGGTCCAA ACTGTCCAGG 
CGCAGATCAG CCCAAATGAG AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758415.1
CDS	217..5643
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDTVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGSSPLFTTCMSESMAEITPSPTLSSPGSEGPEPASQLEEEDIFQDDEEEDDGPQEHPCCAVRSDPFHDRSPLFSVVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRQFLDSDTPSCQSGRGTRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASERFSYKLLHYCTRLTCMLMNMRGKVGYAPSNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_031902555.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902555.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGCTCTC CCCTATTCAC CACATGCATG 
AGCGAGAGCA TGGCCGAAAT TACTCCCTCC CCCACTCTAT CGAGCCCCGG CTCCGAAGGC 
CCGGAGCCGG CCTCCCAGCT TGAAGAGGAG GATATTTTTC AGGATGATGA GGAAGAGGAT 
GATGGACCTC AGGAGCATCC CTGCTGTGCT GTCAGATCAG ACCCGTTTCA CGACCGATCC 
CCCCTGTTCA GTGTAGTGGG AAGGGCTTTT GTGTACCTGA GTAATTTGCT GTACTCTGTC 
CCTTTGGTTC ATCGAGTCGC CATTGTCAGT GAGAAAGGAG AAGTGAAAGG ATTTCTTCGT 
GTTGCTGTTC AGGCCATATC AGCTGACGAG GAAGCTCCTG ATTATGGTTC TGGAGTCCGG 
CAGTCTGGCA CAGCTAAAAT ATCCTTTGAT GATCAACATT TTCAAAAGTT CCAGGCTGAA 
ACCTGCCCAG GAATGACACG CTCAGGCACT TCACAAGAAG AGCTTCGAAT TGTTGAGGGA 
GGACAAGGAC AAAACCCGGA CTCGTGTCCT TCTGCTGACG AAGTGAACAA CAATACCTGT 
GCAGCTTCTC TGCAGTCTTC CTTGTTGGAT AGTCCTACCA AGCCACCCTT TGACCTCTCA 
GACACTATGG GCCACCCTCT GAAACTGGGC AGCATCTTCA CATTCAGAGT GACTGTCTTG 
CAGGCATCGA GTATACCTCC AGAATATGCT GACATATTCT GCCAATTCAA TTTTATTCAT 
CGCCATGATG AGGCCTTTTC AACTGAACCA TTAAAAAACA CAGGCCGAGG GCCACCACTG 
GGCTTCTACC ATGTGCAAAA TATTGCTGTG GAGGTCACTC AGTCATTTGT GGAATATGTG 
AAGAGTCAGC CAATTGTCTT TGAGGTGTTT GGACATTATC AGCAGCATCC TTTCCCTCCT 
CTGTGCAAAG ATGTTCTAAG CCCTCTGCGC TCTTCCAGAC GCCTGCTTCC AAGGGTCATG 
CCTCTGTCTA AACCAGTTCC TGCCACTAAG TTGAGCAGAC AGCGAGTTCC CAGTGTTGGT 
CCCTGTCACT GCAAGTATGA CCTGATGGCT TACTTCCAGA TCTGTGAACT TGAAGCCAAT 
GGAGATTACA TACCTGTGTC AGTAGATCAT GGAGTGGGTT TGCCTTGTCA TGGGACATTT 
CTTCTCCACC AGGGTATCCA GAGACGCATT TCAGTGACAC TGGTGCATGA AGCTGGCGCT 
GAGCTGAAGT GGCTGGAAGT GCGAGAGCTG GTTGTAGGTC GCATCCGGAA TACCCCAGAG 
GCAGATGAGT CTCTAATTGA CCCAAACATC TTGTCACTGA ATATTCTATC TGCGGGATAT 
CGGCCCCCTG AAGGAGGTGA TCGGCAGTTT CTTGATTCTG ATACTCCTAG CTGCCAATCC 
GGGAGGGGCA CCAGGACATT CTATCACTTT GAGGTGGCCT GGGACAGCTC TATGCACAAC 
TCTTTGTTGC TGAATCGTGT CACTCCATAC AGAGAGAAGA TCTACATGAC ATTGTCTGCC 
TATGTTGAGA TGGAAAATTG TACACAGCCT GCTGTGATCA CCAAAGACTT CTGCATGGTT 
TTCTATTCTC GAGATGCCAA ACTCCCAGCT TGTCGCTCCA TTCGGAACCT GTTTGGAACT 
GGCAGCTTAC GAGCCTCTGA AAGATTCTCC TATAAGTTAT TGCATTACTG TACTCGGCTT 
ACATGCATGC TTATGAATAT GAGAGGCAAG GTGGGATATG CACCAAGTAA TCGTGTGACT 
GGAGTTTATG AGATGAGTCT GTGTCGTGTT GCAGAAACCA GTAGTCCAGG GATGCAGCGG 
AGACGGAGAC GTGTGCTAGA CACCTCAGTA GCATATGTAC GTGGGGAGGA AAACCTGGCA 
GGGTGGAGGC CACGGAGTGA CAGCCTTATC CTTGACCACC AGTGGGAACT GGAGAAACTT 
AGCTTGCTTC AGGAGGTGGA GAAAACTCGC CATTACCTGC TGCTGAGAGA GAAACTGGAA 
GCCACACTCA GGGGAGCCAC AGAATCTCTC ACCCCTTCCC CAATTGAGGA GATGCTGCCC 
CTTAGCCCAA CATGTCAGCC GCTTCAGCTC ACAGAGGTTG GATCACCCCG TGCAGAAGAG 
AACCCCCTAG AAACTGCCAG CGAAAGGCAG AAAGAGCTGG CTGCCAAGTG TCTTCGCCTG 
CTCACCCACA CCTTTAACAG AGAGTACAGT TACAGTCATG TATGTGTGAG TGCCAGCGAG 
AGTAAGCTTT CTGAAATGTC AGTTACACTT TTACGGGACC CCTCACTGTC TGGCTTAGGG 
GTCAACTCCC TTACACCATC CAGCACCTGC CCTTCCCTAG TAGAAGGACG TTACTGCAGT 
CCAGAGGCTA GCCCACAACC TATCTCCCGC GTATTCAGCC CTGAGAGAGA GCTGCTGAGC 
CCAGCCGGAG ACCTACTGAA ATCTCCCAGT GGTGAACTGA AGACTCCCAC TCTAAACCTG 
TTTGTACCAG AAATACAAGA AGTCAGAGTT AGTCCAATTG TCTCTAAGAA GGGATACCTA 
CACTTCCTGG AGCCACATAC AGGAGGCTGG GTGAAGAGAT ATGTAGTAGT CAGGCGACCC 
TATGTCTACA TTTACAATAG CGATCGGGAC CCAGTGGAGA GAGCCATCCT TAACCTCTCC 
CAGGCCCATG TTGAATATAG TGAGGACCAA CAAGCTATGC TGAAGACCCC CAACACTTTT 
GCAGTATGCA CACAGCACCG CGGCATCCTG CTGCAGGCCA GCAATGACAA AGACATGCAC 
GACTGGCTCT ACGCCTTTAA CCCTCTCCTG GCGGGCTCCA TCCGGTCCAA ACTGTCCAGG 
CGCAGATCAG CCCAAATGAG AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_031902556.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758416.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X6, mRNA.

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CloneID	OXa58876
Clone ID Related Accession (Same CDS sequence)	XM_031902556.1
Accession Version	XM_031902556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG CAGTTTCTTG ATTCTGATAC TCCTAGCTGC 
CAATCCGGGA GGGGCACCAG GACATTCTAT CACTTTGAGG TGGCCTGGGA CAGCTCTATG 
CACAACTCTT TGTTGCTGAA TCGTGTCACT CCATACAGAG AGAAGATCTA CATGACATTG 
TCTGCCTATG TTGAGATGGA AAATTGTACA CAGCCTGCTG TGATCACCAA AGACTTCTGC 
ATGGTTTTCT ATTCTCGAGA TGCCAAACTC CCAGCTTGTC GCTCCATTCG GAACCTGTTT 
GGAACTGGCA GCTTACGAGC CTCTGAAAGT AATCGTGTGA CTGGAGTTTA TGAGATGAGT 
CTGTGTCGTG TTGCAGAAAC CAGTAGTCCA GGGATGCAGC GGAGACGGAG ACGTGTGCTA 
GACACCTCAG TAGCATATGT ACGTGGGGAG GAAAACCTGG CAGGGTGGAG GCCACGGAGT 
GACAGCCTTA TCCTTGACCA CCAGTGGGAA CTGGAGAAAC TTAGCTTGCT TCAGGAGGTG 
GAGAAAACTC GCCATTACCT GCTGCTGAGA GAGAAACTGG AAGCCACACT CAGGGGAGCC 
ACAGAATCTC TCACCCCTTC CCCAATTGAG GAGATGCTGC CCCTTAGCCC AACATGTCAG 
CCGCTTCAGC TCACAGAGGT TGGATCACCC CGTGCAGAAG AGAACCCCCT AGAAACTGCC 
AGCGAAAGGC AGAAAGAGCT GGCTGCCAAG TGTCTTCGCC TGCTCACCCA CACCTTTAAC 
AGAGAGTACA GTTACAGTCA TGTATGTGTG AGTGCCAGCG AGAGTAAGCT TTCTGAAATG 
TCAGTTACAC TTTTACGGGA CCCCTCACTG TCTGGCTTAG GGGTCAACTC CCTTACACCA 
TCCAGCACCT GCCCTTCCCT AGTAGAAGGA CGTTACTGCA GTCCAGAGGC TAGCCCACAA 
CCTATCTCCC GCGTATTCAG CCCTGAGAGA GAGCTGCTGA GCCCAGCCGG AGACCTACTG 
AAATCTCCCA GTGGTGAACT GAAGACTCCC ACTCTAAACC TGTTTGTACC AGAAATACAA 
GAAGTCAGAG TTAGTCCAAT TGTCTCTAAG AAGGGATACC TACACTTCCT GGAGCCACAT 
ACAGGAGGCT GGGTGAAGAG ATATGTAGTA GTCAGGCGAC CCTATGTCTA CATTTACAAT 
AGCGATCGGG ACCCAGTGGA GAGAGCCATC CTTAACCTCT CCCAGGCCCA TGTTGAATAT 
AGTGAGGACC AACAAGCTAT GCTGAAGACC CCCAACACTT TTGCAGTATG CACACAGCAC 
CGCGGCATCC TGCTGCAGGC CAGCAATGAC AAAGACATGC ACGACTGGCT CTACGCCTTT 
AACCCTCTCC TGGCGGGCTC CATCCGGTCC AAACTGTCCA GGCGCAGATC AGCCCAAATG 
AGAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758416.1
CDS	217..5625
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGSSPLFTTCMSESMAEITPSPTLSSPGSEGPEPASQLEEEDIFQDDEEEDDGPQEHPCCAVRSDPFHDRSPLFSVVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRQFLDSDTPSCQSGRGTRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_031902556.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902556.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG CAGTTTCTTG ATTCTGATAC TCCTAGCTGC 
CAATCCGGGA GGGGCACCAG GACATTCTAT CACTTTGAGG TGGCCTGGGA CAGCTCTATG 
CACAACTCTT TGTTGCTGAA TCGTGTCACT CCATACAGAG AGAAGATCTA CATGACATTG 
TCTGCCTATG TTGAGATGGA AAATTGTACA CAGCCTGCTG TGATCACCAA AGACTTCTGC 
ATGGTTTTCT ATTCTCGAGA TGCCAAACTC CCAGCTTGTC GCTCCATTCG GAACCTGTTT 
GGAACTGGCA GCTTACGAGC CTCTGAAAGT AATCGTGTGA CTGGAGTTTA TGAGATGAGT 
CTGTGTCGTG TTGCAGAAAC CAGTAGTCCA GGGATGCAGC GGAGACGGAG ACGTGTGCTA 
GACACCTCAG TAGCATATGT ACGTGGGGAG GAAAACCTGG CAGGGTGGAG GCCACGGAGT 
GACAGCCTTA TCCTTGACCA CCAGTGGGAA CTGGAGAAAC TTAGCTTGCT TCAGGAGGTG 
GAGAAAACTC GCCATTACCT GCTGCTGAGA GAGAAACTGG AAGCCACACT CAGGGGAGCC 
ACAGAATCTC TCACCCCTTC CCCAATTGAG GAGATGCTGC CCCTTAGCCC AACATGTCAG 
CCGCTTCAGC TCACAGAGGT TGGATCACCC CGTGCAGAAG AGAACCCCCT AGAAACTGCC 
AGCGAAAGGC AGAAAGAGCT GGCTGCCAAG TGTCTTCGCC TGCTCACCCA CACCTTTAAC 
AGAGAGTACA GTTACAGTCA TGTATGTGTG AGTGCCAGCG AGAGTAAGCT TTCTGAAATG 
TCAGTTACAC TTTTACGGGA CCCCTCACTG TCTGGCTTAG GGGTCAACTC CCTTACACCA 
TCCAGCACCT GCCCTTCCCT AGTAGAAGGA CGTTACTGCA GTCCAGAGGC TAGCCCACAA 
CCTATCTCCC GCGTATTCAG CCCTGAGAGA GAGCTGCTGA GCCCAGCCGG AGACCTACTG 
AAATCTCCCA GTGGTGAACT GAAGACTCCC ACTCTAAACC TGTTTGTACC AGAAATACAA 
GAAGTCAGAG TTAGTCCAAT TGTCTCTAAG AAGGGATACC TACACTTCCT GGAGCCACAT 
ACAGGAGGCT GGGTGAAGAG ATATGTAGTA GTCAGGCGAC CCTATGTCTA CATTTACAAT 
AGCGATCGGG ACCCAGTGGA GAGAGCCATC CTTAACCTCT CCCAGGCCCA TGTTGAATAT 
AGTGAGGACC AACAAGCTAT GCTGAAGACC CCCAACACTT TTGCAGTATG CACACAGCAC 
CGCGGCATCC TGCTGCAGGC CAGCAATGAC AAAGACATGC ACGACTGGCT CTACGCCTTT 
AACCCTCTCC TGGCGGGCTC CATCCGGTCC AAACTGTCCA GGCGCAGATC AGCCCAAATG 
AGAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_031902557.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758417.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X7, mRNA.

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CloneID	OXa58877
Clone ID Related Accession (Same CDS sequence)	XM_031902557.1
Accession Version	XM_031902557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG CAGTTTCTTG ATTCTGATAC TCCTAGGACA 
TTCTATCACT TTGAGGTGGC CTGGGACAGC TCTATGCACA ACTCTTTGTT GCTGAATCGT 
GTCACTCCAT ACAGAGAGAA GATCTACATG ACATTGTCTG CCTATGTTGA GATGGAAAAT 
TGTACACAGC CTGCTGTGAT CACCAAAGAC TTCTGCATGG TTTTCTATTC TCGAGATGCC 
AAACTCCCAG CTTGTCGCTC CATTCGGAAC CTGTTTGGAA CTGGCAGCTT ACGAGCCTCT 
GAAAGTAATC GTGTGACTGG AGTTTATGAG ATGAGTCTGT GTCGTGTTGC AGAAACCAGT 
AGTCCAGGGA TGCAGCGGAG ACGGAGACGT GTGCTAGACA CCTCAGTAGC ATATGTACGT 
GGGGAGGAAA ACCTGGCAGG GTGGAGGCCA CGGAGTGACA GCCTTATCCT TGACCACCAG 
TGGGAACTGG AGAAACTTAG CTTGCTTCAG GAGGTGGAGA AAACTCGCCA TTACCTGCTG 
CTGAGAGAGA AACTGGAAGC CACACTCAGG GGAGCCACAG AATCTCTCAC CCCTTCCCCA 
ATTGAGGAGA TGCTGCCCCT TAGCCCAACA TGTCAGCCGC TTCAGCTCAC AGAGGTTGGA 
TCACCCCGTG CAGAAGAGAA CCCCCTAGAA ACTGCCAGCG AAAGGCAGAA AGAGCTGGCT 
GCCAAGTGTC TTCGCCTGCT CACCCACACC TTTAACAGAG AGTACAGTTA CAGTCATGTA 
TGTGTGAGTG CCAGCGAGAG TAAGCTTTCT GAAATGTCAG TTACACTTTT ACGGGACCCC 
TCACTGTCTG GCTTAGGGGT CAACTCCCTT ACACCATCCA GCACCTGCCC TTCCCTAGTA 
GAAGGACGTT ACTGCAGTCC AGAGGCTAGC CCACAACCTA TCTCCCGCGT ATTCAGCCCT 
GAGAGAGAGC TGCTGAGCCC AGCCGGAGAC CTACTGAAAT CTCCCAGTGG TGAACTGAAG 
ACTCCCACTC TAAACCTGTT TGTACCAGAA ATACAAGAAG TCAGAGTTAG TCCAATTGTC 
TCTAAGAAGG GATACCTACA CTTCCTGGAG CCACATACAG GAGGCTGGGT GAAGAGATAT 
GTAGTAGTCA GGCGACCCTA TGTCTACATT TACAATAGCG ATCGGGACCC AGTGGAGAGA 
GCCATCCTTA ACCTCTCCCA GGCCCATGTT GAATATAGTG AGGACCAACA AGCTATGCTG 
AAGACCCCCA ACACTTTTGC AGTATGCACA CAGCACCGCG GCATCCTGCT GCAGGCCAGC 
AATGACAAAG ACATGCACGA CTGGCTCTAC GCCTTTAACC CTCTCCTGGC GGGCTCCATC 
CGGTCCAAAC TGTCCAGGCG CAGATCAGCC CAAATGAGAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758417.1
CDS	217..5601
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGSSPLFTTCMSESMAEITPSPTLSSPGSEGPEPASQLEEEDIFQDDEEEDDGPQEHPCCAVRSDPFHDRSPLFSVVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRQFLDSDTPRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_031902557.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902557.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG CAGTTTCTTG ATTCTGATAC TCCTAGGACA 
TTCTATCACT TTGAGGTGGC CTGGGACAGC TCTATGCACA ACTCTTTGTT GCTGAATCGT 
GTCACTCCAT ACAGAGAGAA GATCTACATG ACATTGTCTG CCTATGTTGA GATGGAAAAT 
TGTACACAGC CTGCTGTGAT CACCAAAGAC TTCTGCATGG TTTTCTATTC TCGAGATGCC 
AAACTCCCAG CTTGTCGCTC CATTCGGAAC CTGTTTGGAA CTGGCAGCTT ACGAGCCTCT 
GAAAGTAATC GTGTGACTGG AGTTTATGAG ATGAGTCTGT GTCGTGTTGC AGAAACCAGT 
AGTCCAGGGA TGCAGCGGAG ACGGAGACGT GTGCTAGACA CCTCAGTAGC ATATGTACGT 
GGGGAGGAAA ACCTGGCAGG GTGGAGGCCA CGGAGTGACA GCCTTATCCT TGACCACCAG 
TGGGAACTGG AGAAACTTAG CTTGCTTCAG GAGGTGGAGA AAACTCGCCA TTACCTGCTG 
CTGAGAGAGA AACTGGAAGC CACACTCAGG GGAGCCACAG AATCTCTCAC CCCTTCCCCA 
ATTGAGGAGA TGCTGCCCCT TAGCCCAACA TGTCAGCCGC TTCAGCTCAC AGAGGTTGGA 
TCACCCCGTG CAGAAGAGAA CCCCCTAGAA ACTGCCAGCG AAAGGCAGAA AGAGCTGGCT 
GCCAAGTGTC TTCGCCTGCT CACCCACACC TTTAACAGAG AGTACAGTTA CAGTCATGTA 
TGTGTGAGTG CCAGCGAGAG TAAGCTTTCT GAAATGTCAG TTACACTTTT ACGGGACCCC 
TCACTGTCTG GCTTAGGGGT CAACTCCCTT ACACCATCCA GCACCTGCCC TTCCCTAGTA 
GAAGGACGTT ACTGCAGTCC AGAGGCTAGC CCACAACCTA TCTCCCGCGT ATTCAGCCCT 
GAGAGAGAGC TGCTGAGCCC AGCCGGAGAC CTACTGAAAT CTCCCAGTGG TGAACTGAAG 
ACTCCCACTC TAAACCTGTT TGTACCAGAA ATACAAGAAG TCAGAGTTAG TCCAATTGTC 
TCTAAGAAGG GATACCTACA CTTCCTGGAG CCACATACAG GAGGCTGGGT GAAGAGATAT 
GTAGTAGTCA GGCGACCCTA TGTCTACATT TACAATAGCG ATCGGGACCC AGTGGAGAGA 
GCCATCCTTA ACCTCTCCCA GGCCCATGTT GAATATAGTG AGGACCAACA AGCTATGCTG 
AAGACCCCCA ACACTTTTGC AGTATGCACA CAGCACCGCG GCATCCTGCT GCAGGCCAGC 
AATGACAAAG ACATGCACGA CTGGCTCTAC GCCTTTAACC CTCTCCTGGC GGGCTCCATC 
CGGTCCAAAC TGTCCAGGCG CAGATCAGCC CAAATGAGAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_031902558.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758418.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X8, mRNA.

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CloneID	OXa58878
Clone ID Related Accession (Same CDS sequence)	XM_031902558.1
Accession Version	XM_031902558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG ACATTCTATC ACTTTGAGGT GGCCTGGGAC 
AGCTCTATGC ACAACTCTTT GTTGCTGAAT CGTGTCACTC CATACAGAGA GAAGATCTAC 
ATGACATTGT CTGCCTATGT TGAGATGGAA AATTGTACAC AGCCTGCTGT GATCACCAAA 
GACTTCTGCA TGGTTTTCTA TTCTCGAGAT GCCAAACTCC CAGCTTGTCG CTCCATTCGG 
AACCTGTTTG GAACTGGCAG CTTACGAGCC TCTGAAAGTA ATCGTGTGAC TGGAGTTTAT 
GAGATGAGTC TGTGTCGTGT TGCAGAAACC AGTAGTCCAG GGATGCAGCG GAGACGGAGA 
CGTGTGCTAG ACACCTCAGT AGCATATGTA CGTGGGGAGG AAAACCTGGC AGGGTGGAGG 
CCACGGAGTG ACAGCCTTAT CCTTGACCAC CAGTGGGAAC TGGAGAAACT TAGCTTGCTT 
CAGGAGGTGG AGAAAACTCG CCATTACCTG CTGCTGAGAG AGAAACTGGA AGCCACACTC 
AGGGGAGCCA CAGAATCTCT CACCCCTTCC CCAATTGAGG AGATGCTGCC CCTTAGCCCA 
ACATGTCAGC CGCTTCAGCT CACAGAGGTT GGATCACCCC GTGCAGAAGA GAACCCCCTA 
GAAACTGCCA GCGAAAGGCA GAAAGAGCTG GCTGCCAAGT GTCTTCGCCT GCTCACCCAC 
ACCTTTAACA GAGAGTACAG TTACAGTCAT GTATGTGTGA GTGCCAGCGA GAGTAAGCTT 
TCTGAAATGT CAGTTACACT TTTACGGGAC CCCTCACTGT CTGGCTTAGG GGTCAACTCC 
CTTACACCAT CCAGCACCTG CCCTTCCCTA GTAGAAGGAC GTTACTGCAG TCCAGAGGCT 
AGCCCACAAC CTATCTCCCG CGTATTCAGC CCTGAGAGAG AGCTGCTGAG CCCAGCCGGA 
GACCTACTGA AATCTCCCAG TGGTGAACTG AAGACTCCCA CTCTAAACCT GTTTGTACCA 
GAAATACAAG AAGTCAGAGT TAGTCCAATT GTCTCTAAGA AGGGATACCT ACACTTCCTG 
GAGCCACATA CAGGAGGCTG GGTGAAGAGA TATGTAGTAG TCAGGCGACC CTATGTCTAC 
ATTTACAATA GCGATCGGGA CCCAGTGGAG AGAGCCATCC TTAACCTCTC CCAGGCCCAT 
GTTGAATATA GTGAGGACCA ACAAGCTATG CTGAAGACCC CCAACACTTT TGCAGTATGC 
ACACAGCACC GCGGCATCCT GCTGCAGGCC AGCAATGACA AAGACATGCA CGACTGGCTC 
TACGCCTTTA ACCCTCTCCT GGCGGGCTCC ATCCGGTCCA AACTGTCCAG GCGCAGATCA 
GCCCAAATGA GAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758418.1
CDS	217..5574
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGSSPLFTTCMSESMAEITPSPTLSSPGSEGPEPASQLEEEDIFQDDEEEDDGPQEHPCCAVRSDPFHDRSPLFSVVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_031902558.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902558.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GCTCTCCCCT ATTCACCACA 
TGCATGAGCG AGAGCATGGC CGAAATTACT CCCTCCCCCA CTCTATCGAG CCCCGGCTCC 
GAAGGCCCGG AGCCGGCCTC CCAGCTTGAA GAGGAGGATA TTTTTCAGGA TGATGAGGAA 
GAGGATGATG GACCTCAGGA GCATCCCTGC TGTGCTGTCA GATCAGACCC GTTTCACGAC 
CGATCCCCCC TGTTCAGTGT AGTGGGAAGG GCTTTTGTGT ACCTGAGTAA TTTGCTGTAC 
TCTGTCCCTT TGGTTCATCG AGTCGCCATT GTCAGTGAGA AAGGAGAAGT GAAAGGATTT 
CTTCGTGTTG CTGTTCAGGC CATATCAGCT GACGAGGAAG CTCCTGATTA TGGTTCTGGA 
GTCCGGCAGT CTGGCACAGC TAAAATATCC TTTGATGATC AACATTTTCA AAAGTTCCAG 
GCTGAAACCT GCCCAGGAAT GACACGCTCA GGCACTTCAC AAGAAGAGCT TCGAATTGTT 
GAGGGAGGAC AAGGACAAAA CCCGGACTCG TGTCCTTCTG CTGACGAAGT GAACAACAAT 
ACCTGTGCAG CTTCTCTGCA GTCTTCCTTG TTGGATAGTC CTACCAAGCC ACCCTTTGAC 
CTCTCAGACA CTATGGGCCA CCCTCTGAAA CTGGGCAGCA TCTTCACATT CAGAGTGACT 
GTCTTGCAGG CATCGAGTAT ACCTCCAGAA TATGCTGACA TATTCTGCCA ATTCAATTTT 
ATTCATCGCC ATGATGAGGC CTTTTCAACT GAACCATTAA AAAACACAGG CCGAGGGCCA 
CCACTGGGCT TCTACCATGT GCAAAATATT GCTGTGGAGG TCACTCAGTC ATTTGTGGAA 
TATGTGAAGA GTCAGCCAAT TGTCTTTGAG GTGTTTGGAC ATTATCAGCA GCATCCTTTC 
CCTCCTCTGT GCAAAGATGT TCTAAGCCCT CTGCGCTCTT CCAGACGCCT GCTTCCAAGG 
GTCATGCCTC TGTCTAAACC AGTTCCTGCC ACTAAGTTGA GCAGACAGCG AGTTCCCAGT 
GTTGGTCCCT GTCACTGCAA GTATGACCTG ATGGCTTACT TCCAGATCTG TGAACTTGAA 
GCCAATGGAG ATTACATACC TGTGTCAGTA GATCATGGAG TGGGTTTGCC TTGTCATGGG 
ACATTTCTTC TCCACCAGGG TATCCAGAGA CGCATTTCAG TGACACTGGT GCATGAAGCT 
GGCGCTGAGC TGAAGTGGCT GGAAGTGCGA GAGCTGGTTG TAGGTCGCAT CCGGAATACC 
CCAGAGGCAG ATGAGTCTCT AATTGACCCA AACATCTTGT CACTGAATAT TCTATCTGCG 
GGATATCGGC CCCCTGAAGG AGGTGATCGG ACATTCTATC ACTTTGAGGT GGCCTGGGAC 
AGCTCTATGC ACAACTCTTT GTTGCTGAAT CGTGTCACTC CATACAGAGA GAAGATCTAC 
ATGACATTGT CTGCCTATGT TGAGATGGAA AATTGTACAC AGCCTGCTGT GATCACCAAA 
GACTTCTGCA TGGTTTTCTA TTCTCGAGAT GCCAAACTCC CAGCTTGTCG CTCCATTCGG 
AACCTGTTTG GAACTGGCAG CTTACGAGCC TCTGAAAGTA ATCGTGTGAC TGGAGTTTAT 
GAGATGAGTC TGTGTCGTGT TGCAGAAACC AGTAGTCCAG GGATGCAGCG GAGACGGAGA 
CGTGTGCTAG ACACCTCAGT AGCATATGTA CGTGGGGAGG AAAACCTGGC AGGGTGGAGG 
CCACGGAGTG ACAGCCTTAT CCTTGACCAC CAGTGGGAAC TGGAGAAACT TAGCTTGCTT 
CAGGAGGTGG AGAAAACTCG CCATTACCTG CTGCTGAGAG AGAAACTGGA AGCCACACTC 
AGGGGAGCCA CAGAATCTCT CACCCCTTCC CCAATTGAGG AGATGCTGCC CCTTAGCCCA 
ACATGTCAGC CGCTTCAGCT CACAGAGGTT GGATCACCCC GTGCAGAAGA GAACCCCCTA 
GAAACTGCCA GCGAAAGGCA GAAAGAGCTG GCTGCCAAGT GTCTTCGCCT GCTCACCCAC 
ACCTTTAACA GAGAGTACAG TTACAGTCAT GTATGTGTGA GTGCCAGCGA GAGTAAGCTT 
TCTGAAATGT CAGTTACACT TTTACGGGAC CCCTCACTGT CTGGCTTAGG GGTCAACTCC 
CTTACACCAT CCAGCACCTG CCCTTCCCTA GTAGAAGGAC GTTACTGCAG TCCAGAGGCT 
AGCCCACAAC CTATCTCCCG CGTATTCAGC CCTGAGAGAG AGCTGCTGAG CCCAGCCGGA 
GACCTACTGA AATCTCCCAG TGGTGAACTG AAGACTCCCA CTCTAAACCT GTTTGTACCA 
GAAATACAAG AAGTCAGAGT TAGTCCAATT GTCTCTAAGA AGGGATACCT ACACTTCCTG 
GAGCCACATA CAGGAGGCTG GGTGAAGAGA TATGTAGTAG TCAGGCGACC CTATGTCTAC 
ATTTACAATA GCGATCGGGA CCCAGTGGAG AGAGCCATCC TTAACCTCTC CCAGGCCCAT 
GTTGAATATA GTGAGGACCA ACAAGCTATG CTGAAGACCC CCAACACTTT TGCAGTATGC 
ACACAGCACC GCGGCATCCT GCTGCAGGCC AGCAATGACA AAGACATGCA CGACTGGCTC 
TACGCCTTTA ACCCTCTCCT GGCGGGCTCC ATCCGGTCCA AACTGTCCAG GCGCAGATCA 
GCCCAAATGA GAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_031902559.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758419.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X9, mRNA.

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CloneID	OXa58879
Clone ID Related Accession (Same CDS sequence)	XM_031902559.1
Accession Version	XM_031902559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GGGCTTTTGT GTACCTGAGT 
AATTTGCTGT ACTCTGTCCC TTTGGTTCAT CGAGTCGCCA TTGTCAGTGA GAAAGGAGAA 
GTGAAAGGAT TTCTTCGTGT TGCTGTTCAG GCCATATCAG CTGACGAGGA AGCTCCTGAT 
TATGGTTCTG GAGTCCGGCA GTCTGGCACA GCTAAAATAT CCTTTGATGA TCAACATTTT 
CAAAAGTTCC AGGCTGAAAC CTGCCCAGGA ATGACACGCT CAGGCACTTC ACAAGAAGAG 
CTTCGAATTG TTGAGGGAGG ACAAGGACAA AACCCGGACT CGTGTCCTTC TGCTGACGAA 
GTGAACAACA ATACCTGTGC AGCTTCTCTG CAGTCTTCCT TGTTGGATAG TCCTACCAAG 
CCACCCTTTG ACCTCTCAGA CACTATGGGC CACCCTCTGA AACTGGGCAG CATCTTCACA 
TTCAGAGTGA CTGTCTTGCA GGCATCGAGT ATACCTCCAG AATATGCTGA CATATTCTGC 
CAATTCAATT TTATTCATCG CCATGATGAG GCCTTTTCAA CTGAACCATT AAAAAACACA 
GGCCGAGGGC CACCACTGGG CTTCTACCAT GTGCAAAATA TTGCTGTGGA GGTCACTCAG 
TCATTTGTGG AATATGTGAA GAGTCAGCCA ATTGTCTTTG AGGTGTTTGG ACATTATCAG 
CAGCATCCTT TCCCTCCTCT GTGCAAAGAT GTTCTAAGCC CTCTGCGCTC TTCCAGACGC 
CTGCTTCCAA GGGTCATGCC TCTGTCTAAA CCAGTTCCTG CCACTAAGTT GAGCAGACAG 
CGAGTTCCCA GTGTTGGTCC CTGTCACTGC AAGTATGACC TGATGGCTTA CTTCCAGATC 
TGTGAACTTG AAGCCAATGG AGATTACATA CCTGTGTCAG TAGATCATGG AGTGGGTTTG 
CCTTGTCATG GGACATTTCT TCTCCACCAG GGTATCCAGA GACGCATTTC AGTGACACTG 
GTGCATGAAG CTGGCGCTGA GCTGAAGTGG CTGGAAGTGC GAGAGCTGGT TGTAGGTCGC 
ATCCGGAATA CCCCAGAGGC AGATGAGTCT CTAATTGACC CAAACATCTT GTCACTGAAT 
ATTCTATCTG CGGGATATCG GCCCCCTGAA GGAGGTGATC GGCAGTTTCT TGATTCTGAT 
ACTCCTAGCT GCCAATCCGG GAGGGGCACC AGGACATTCT ATCACTTTGA GGTGGCCTGG 
GACAGCTCTA TGCACAACTC TTTGTTGCTG AATCGTGTCA CTCCATACAG AGAGAAGATC 
TACATGACAT TGTCTGCCTA TGTTGAGATG GAAAATTGTA CACAGCCTGC TGTGATCACC 
AAAGACTTCT GCATGGTTTT CTATTCTCGA GATGCCAAAC TCCCAGCTTG TCGCTCCATT 
CGGAACCTGT TTGGAACTGG CAGCTTACGA GCCTCTGAAA GATTCTCCTA TAAGTTATTG 
CATTACTGTA CTCGGCTTAC ATGCATGCTT ATGAATATGA GAGGCAAGGT GGGATATGCA 
CCAAGTAATC GTGTGACTGG AGTTTATGAG ATGAGTCTGT GTCGTGTTGC AGAAACCAGT 
AGTCCAGGGA TGCAGCGGAG ACGGAGACGT GTGCTAGACA CCTCAGTAGC ATATGTACGT 
GGGGAGGAAA ACCTGGCAGG GTGGAGGCCA CGGAGTGACA GCCTTATCCT TGACCACCAG 
TGGGAACTGG AGAAACTTAG CTTGCTTCAG GAGGTGGAGA AAACTCGCCA TTACCTGCTG 
CTGAGAGAGA AACTGGAAGC CACACTCAGG GGAGCCACAG AATCTCTCAC CCCTTCCCCA 
ATTGAGGAGA TGCTGCCCCT TAGCCCAACA TGTCAGCCGC TTCAGCTCAC AGAGGTTGGA 
TCACCCCGTG CAGAAGAGAA CCCCCTAGAA ACTGCCAGCG AAAGGCAGAA AGAGCTGGCT 
GCCAAGTGTC TTCGCCTGCT CACCCACACC TTTAACAGAG AGTACAGTTA CAGTCATGTA 
TGTGTGAGTG CCAGCGAGAG TAAGCTTTCT GAAATGTCAG TTACACTTTT ACGGGACCCC 
TCACTGTCTG GCTTAGGGGT CAACTCCCTT ACACCATCCA GCACCTGCCC TTCCCTAGTA 
GAAGGACGTT ACTGCAGTCC AGAGGCTAGC CCACAACCTA TCTCCCGCGT ATTCAGCCCT 
GAGAGAGAGC TGCTGAGCCC AGCCGGAGAC CTACTGAAAT CTCCCAGTGG TGAACTGAAG 
ACTCCCACTC TAAACCTGTT TGTACCAGAA ATACAAGAAG TCAGAGTTAG TCCAATTGTC 
TCTAAGAAGG GATACCTACA CTTCCTGGAG CCACATACAG GAGGCTGGGT GAAGAGATAT 
GTAGTAGTCA GGCGACCCTA TGTCTACATT TACAATAGCG ATCGGGACCC AGTGGAGAGA 
GCCATCCTTA ACCTCTCCCA GGCCCATGTT GAATATAGTG AGGACCAACA AGCTATGCTG 
AAGACCCCCA ACACTTTTGC AGTATGCACA CAGCACCGCG GCATCCTGCT GCAGGCCAGC 
AATGACAAAG ACATGCACGA CTGGCTCTAC GCCTTTAACC CTCTCCTGGC GGGCTCCATC 
CGGTCCAAAC TGTCCAGGCG CAGATCAGCC CAAATGAGAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758419.1
CDS	217..5481
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDNVCPVDNGNVLHKRHAADNLFTAVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRQFLDSDTPSCQSGRGTRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASERFSYKLLHYCTRLTCMLMNMRGKVGYAPSNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_031902559.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902559.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA ATGTTTGTCC TGTGGATAAC 
GGGAATGTGC TACACAAACG TCATGCTGCT GACAATCTCT TCACAGCCGT TAATGCCCTG 
ACTGGTATGA GTCCTTCGTC TTCCATGTCT GGTTTGTCCA GCCGAGCTGC ATCTGTCACC 
AGCTTACACG AGCGCCTGGC ATTCACACCT GGCAGTGAAG AAGCCATTGA GAGACTCAAG 
GAGACCGAAA AAATTATAGC AGAATTGAAT GAGACATGGG AGGAAAAACT GCGCAGGACT 
GAAGCTATTC GCATGGACAG AGAAGCTTTG CTGGCAGAGA TGGGAGTAGC TATGAGAGAA 
GATGGAGGAA CACTTGGAGT CTTTTCACCT AAAAAGACTC CTCACCTTGT AAATCTTAAT 
GAAGATCCTC TAATGTCTGA GTGTCTATTA TATTACATCA AAGATGGCAT TACCAGGGTT 
GGGCGTGAGG ATGCAACAAT GAGACAAGAC ATTGTGCTGA GTGGCCACTT TATTCGTGAT 
GAGCATTGTA TTTTCCGGAG TGATGTATTG GCTTGCGGAT CAGTGGTGGT AACTCTTGAG 
CCCTGTGATG GAGCTGACAC ATATGTAAAT GGCAAGAAGC TGACAGATCC TTGTATTCTG 
AGATCCGGAA ACAGAATTAT TATGGGCAAG AGTCACGTAT TCCGCTTCAA CCACCCTGAG 
CAAGCGCGCC AGGAAAGGGA ACGCACGCCC TGTGCCGAAA CCCCATCTGA ACCTGTGGAC 
TGGGCATTTG CTCAGAGAGA GCTCCTGGAG AAGCAAGGAA TTGATATGAA ACAAGAGATG 
GATCAGAGGC TCCAAGAACT GGAAGATCAA TACAGAAAAG AACGTGAAGA AGCAAATTAC 
CTTCTGGAAC AGCAGCGACT GGACTATGAA AGCAGACTGG AAGCATTACA GAGGCAGATG 
GATTCTAGAT ATTTTACCGA AGTGAACGAG GAAGAAGAAG AACCTGAAGA TGAAGTTCAG 
TGGACTGAAC AGGAATATGA TCTGGCACTC TGGGCTTTTC GGAAGTGGAA GTGGTACCAG 
TTTACATCAC TCCGAGATCA ACTTTGGGGA AATGCCATCT TCCTTAAAGA AGCCAATGCT 
ATCAGTGTGG AATTGAAGAA AAAGGTACAG TTCCAGTTTG TTCTGCTGAC AGACACTTTG 
TATTCTCCTC TCCCTCCGGA CCTGCTGCCG CCTGATGCCC ATACTGAACG AGAGAAACGT 
CCTTTCCCAA GAACCATTGT AGCTGTGGAG GTTCAGGATC AGAAAAATGG TGCAACTCAC 
TACTGGACCT TAGAAAAACT CAGGCAACGT CTGGACATGA TGAGGGAGAT GTATGACCGG 
GCTGCAGAGC TTCCTTCTGG TCTGACTGAG GACTGTGATA ATGTGGTGAC TGGAGGAGAT 
CCATTTTATG ACCGATTCCC TTGGTTCCGA CTGGTGGGCA GGGCTTTTGT GTACCTGAGT 
AATTTGCTGT ACTCTGTCCC TTTGGTTCAT CGAGTCGCCA TTGTCAGTGA GAAAGGAGAA 
GTGAAAGGAT TTCTTCGTGT TGCTGTTCAG GCCATATCAG CTGACGAGGA AGCTCCTGAT 
TATGGTTCTG GAGTCCGGCA GTCTGGCACA GCTAAAATAT CCTTTGATGA TCAACATTTT 
CAAAAGTTCC AGGCTGAAAC CTGCCCAGGA ATGACACGCT CAGGCACTTC ACAAGAAGAG 
CTTCGAATTG TTGAGGGAGG ACAAGGACAA AACCCGGACT CGTGTCCTTC TGCTGACGAA 
GTGAACAACA ATACCTGTGC AGCTTCTCTG CAGTCTTCCT TGTTGGATAG TCCTACCAAG 
CCACCCTTTG ACCTCTCAGA CACTATGGGC CACCCTCTGA AACTGGGCAG CATCTTCACA 
TTCAGAGTGA CTGTCTTGCA GGCATCGAGT ATACCTCCAG AATATGCTGA CATATTCTGC 
CAATTCAATT TTATTCATCG CCATGATGAG GCCTTTTCAA CTGAACCATT AAAAAACACA 
GGCCGAGGGC CACCACTGGG CTTCTACCAT GTGCAAAATA TTGCTGTGGA GGTCACTCAG 
TCATTTGTGG AATATGTGAA GAGTCAGCCA ATTGTCTTTG AGGTGTTTGG ACATTATCAG 
CAGCATCCTT TCCCTCCTCT GTGCAAAGAT GTTCTAAGCC CTCTGCGCTC TTCCAGACGC 
CTGCTTCCAA GGGTCATGCC TCTGTCTAAA CCAGTTCCTG CCACTAAGTT GAGCAGACAG 
CGAGTTCCCA GTGTTGGTCC CTGTCACTGC AAGTATGACC TGATGGCTTA CTTCCAGATC 
TGTGAACTTG AAGCCAATGG AGATTACATA CCTGTGTCAG TAGATCATGG AGTGGGTTTG 
CCTTGTCATG GGACATTTCT TCTCCACCAG GGTATCCAGA GACGCATTTC AGTGACACTG 
GTGCATGAAG CTGGCGCTGA GCTGAAGTGG CTGGAAGTGC GAGAGCTGGT TGTAGGTCGC 
ATCCGGAATA CCCCAGAGGC AGATGAGTCT CTAATTGACC CAAACATCTT GTCACTGAAT 
ATTCTATCTG CGGGATATCG GCCCCCTGAA GGAGGTGATC GGCAGTTTCT TGATTCTGAT 
ACTCCTAGCT GCCAATCCGG GAGGGGCACC AGGACATTCT ATCACTTTGA GGTGGCCTGG 
GACAGCTCTA TGCACAACTC TTTGTTGCTG AATCGTGTCA CTCCATACAG AGAGAAGATC 
TACATGACAT TGTCTGCCTA TGTTGAGATG GAAAATTGTA CACAGCCTGC TGTGATCACC 
AAAGACTTCT GCATGGTTTT CTATTCTCGA GATGCCAAAC TCCCAGCTTG TCGCTCCATT 
CGGAACCTGT TTGGAACTGG CAGCTTACGA GCCTCTGAAA GATTCTCCTA TAAGTTATTG 
CATTACTGTA CTCGGCTTAC ATGCATGCTT ATGAATATGA GAGGCAAGGT GGGATATGCA 
CCAAGTAATC GTGTGACTGG AGTTTATGAG ATGAGTCTGT GTCGTGTTGC AGAAACCAGT 
AGTCCAGGGA TGCAGCGGAG ACGGAGACGT GTGCTAGACA CCTCAGTAGC ATATGTACGT 
GGGGAGGAAA ACCTGGCAGG GTGGAGGCCA CGGAGTGACA GCCTTATCCT TGACCACCAG 
TGGGAACTGG AGAAACTTAG CTTGCTTCAG GAGGTGGAGA AAACTCGCCA TTACCTGCTG 
CTGAGAGAGA AACTGGAAGC CACACTCAGG GGAGCCACAG AATCTCTCAC CCCTTCCCCA 
ATTGAGGAGA TGCTGCCCCT TAGCCCAACA TGTCAGCCGC TTCAGCTCAC AGAGGTTGGA 
TCACCCCGTG CAGAAGAGAA CCCCCTAGAA ACTGCCAGCG AAAGGCAGAA AGAGCTGGCT 
GCCAAGTGTC TTCGCCTGCT CACCCACACC TTTAACAGAG AGTACAGTTA CAGTCATGTA 
TGTGTGAGTG CCAGCGAGAG TAAGCTTTCT GAAATGTCAG TTACACTTTT ACGGGACCCC 
TCACTGTCTG GCTTAGGGGT CAACTCCCTT ACACCATCCA GCACCTGCCC TTCCCTAGTA 
GAAGGACGTT ACTGCAGTCC AGAGGCTAGC CCACAACCTA TCTCCCGCGT ATTCAGCCCT 
GAGAGAGAGC TGCTGAGCCC AGCCGGAGAC CTACTGAAAT CTCCCAGTGG TGAACTGAAG 
ACTCCCACTC TAAACCTGTT TGTACCAGAA ATACAAGAAG TCAGAGTTAG TCCAATTGTC 
TCTAAGAAGG GATACCTACA CTTCCTGGAG CCACATACAG GAGGCTGGGT GAAGAGATAT 
GTAGTAGTCA GGCGACCCTA TGTCTACATT TACAATAGCG ATCGGGACCC AGTGGAGAGA 
GCCATCCTTA ACCTCTCCCA GGCCCATGTT GAATATAGTG AGGACCAACA AGCTATGCTG 
AAGACCCCCA ACACTTTTGC AGTATGCACA CAGCACCGCG GCATCCTGCT GCAGGCCAGC 
AATGACAAAG ACATGCACGA CTGGCTCTAC GCCTTTAACC CTCTCCTGGC GGGCTCCATC 
CGGTCCAAAC TGTCCAGGCG CAGATCAGCC CAAATGAGAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_031902560.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758420.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X10, mRNA.

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CloneID	OXa58880
Clone ID Related Accession (Same CDS sequence)	XM_031902560.1
Accession Version	XM_031902560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
TTCCAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGCAGT TTCTTGATTC TGATACTCCT 
AGCTGCCAAT CCGGGAGGGG CACCAGGACA TTCTATCACT TTGAGGTGGC CTGGGACAGC 
TCTATGCACA ACTCTTTGTT GCTGAATCGT GTCACTCCAT ACAGAGAGAA GATCTACATG 
ACATTGTCTG CCTATGTTGA GATGGAAAAT TGTACACAGC CTGCTGTGAT CACCAAAGAC 
TTCTGCATGG TTTTCTATTC TCGAGATGCC AAACTCCCAG CTTGTCGCTC CATTCGGAAC 
CTGTTTGGAA CTGGCAGCTT ACGAGCCTCT GAAAGATTCT CCTATAAGTT ATTGCATTAC 
TGTACTCGGC TTACATGCAT GCTTATGAAT ATGAGAGGCA AGGTGGGATA TGCACCAAGT 
AATCGTGTGA CTGGAGTTTA TGAGATGAGT CTGTGTCGTG TTGCAGAAAC CAGTAGTCCA 
GGGATGCAGC GGAGACGGAG ACGTGTGCTA GACACCTCAG TAGCATATGT ACGTGGGGAG 
GAAAACCTGG CAGGGTGGAG GCCACGGAGT GACAGCCTTA TCCTTGACCA CCAGTGGGAA 
CTGGAGAAAC TTAGCTTGCT TCAGGAGGTG GAGAAAACTC GCCATTACCT GCTGCTGAGA 
GAGAAACTGG AAGCCACACT CAGGGGAGCC ACAGAATCTC TCACCCCTTC CCCAATTGAG 
GAGATGCTGC CCCTTAGCCC AACATGTCAG CCGCTTCAGC TCACAGAGGT TGGATCACCC 
CGTGCAGAAG AGAACCCCCT AGAAACTGCC AGCGAAAGGC AGAAAGAGCT GGCTGCCAAG 
TGTCTTCGCC TGCTCACCCA CACCTTTAAC AGAGAGTACA GTTACAGTCA TGTATGTGTG 
AGTGCCAGCG AGAGTAAGCT TTCTGAAATG TCAGTTACAC TTTTACGGGA CCCCTCACTG 
TCTGGCTTAG GGGTCAACTC CCTTACACCA TCCAGCACCT GCCCTTCCCT AGTAGAAGGA 
CGTTACTGCA GTCCAGAGGC TAGCCCACAA CCTATCTCCC GCGTATTCAG CCCTGAGAGA 
GAGCTGCTGA GCCCAGCCGG AGACCTACTG AAATCTCCCA GTGGTGAACT GAAGACTCCC 
ACTCTAAACC TGTTTGTACC AGAAATACAA GAAGTCAGAG TTAGTCCAAT TGTCTCTAAG 
AAGGGATACC TACACTTCCT GGAGCCACAT ACAGGAGGCT GGGTGAAGAG ATATGTAGTA 
GTCAGGCGAC CCTATGTCTA CATTTACAAT AGCGATCGGG ACCCAGTGGA GAGAGCCATC 
CTTAACCTCT CCCAGGCCCA TGTTGAATAT AGTGAGGACC AACAAGCTAT GCTGAAGACC 
CCCAACACTT TTGCAGTATG CACACAGCAC CGCGGCATCC TGCTGCAGGC CAGCAATGAC 
AAAGACATGC ACGACTGGCT CTACGCCTTT AACCCTCTCC TGGCGGGCTC CATCCGGTCC 
AAACTGTCCA GGCGCAGATC AGCCCAAATG AGAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758420.1
CDS	217..5415
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDTVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRQFLDSDTPSCQSGRGTRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASERFSYKLLHYCTRLTCMLMNMRGKVGYAPSNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_031902560.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902560.1

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
TTCCAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGCAGT TTCTTGATTC TGATACTCCT 
AGCTGCCAAT CCGGGAGGGG CACCAGGACA TTCTATCACT TTGAGGTGGC CTGGGACAGC 
TCTATGCACA ACTCTTTGTT GCTGAATCGT GTCACTCCAT ACAGAGAGAA GATCTACATG 
ACATTGTCTG CCTATGTTGA GATGGAAAAT TGTACACAGC CTGCTGTGAT CACCAAAGAC 
TTCTGCATGG TTTTCTATTC TCGAGATGCC AAACTCCCAG CTTGTCGCTC CATTCGGAAC 
CTGTTTGGAA CTGGCAGCTT ACGAGCCTCT GAAAGATTCT CCTATAAGTT ATTGCATTAC 
TGTACTCGGC TTACATGCAT GCTTATGAAT ATGAGAGGCA AGGTGGGATA TGCACCAAGT 
AATCGTGTGA CTGGAGTTTA TGAGATGAGT CTGTGTCGTG TTGCAGAAAC CAGTAGTCCA 
GGGATGCAGC GGAGACGGAG ACGTGTGCTA GACACCTCAG TAGCATATGT ACGTGGGGAG 
GAAAACCTGG CAGGGTGGAG GCCACGGAGT GACAGCCTTA TCCTTGACCA CCAGTGGGAA 
CTGGAGAAAC TTAGCTTGCT TCAGGAGGTG GAGAAAACTC GCCATTACCT GCTGCTGAGA 
GAGAAACTGG AAGCCACACT CAGGGGAGCC ACAGAATCTC TCACCCCTTC CCCAATTGAG 
GAGATGCTGC CCCTTAGCCC AACATGTCAG CCGCTTCAGC TCACAGAGGT TGGATCACCC 
CGTGCAGAAG AGAACCCCCT AGAAACTGCC AGCGAAAGGC AGAAAGAGCT GGCTGCCAAG 
TGTCTTCGCC TGCTCACCCA CACCTTTAAC AGAGAGTACA GTTACAGTCA TGTATGTGTG 
AGTGCCAGCG AGAGTAAGCT TTCTGAAATG TCAGTTACAC TTTTACGGGA CCCCTCACTG 
TCTGGCTTAG GGGTCAACTC CCTTACACCA TCCAGCACCT GCCCTTCCCT AGTAGAAGGA 
CGTTACTGCA GTCCAGAGGC TAGCCCACAA CCTATCTCCC GCGTATTCAG CCCTGAGAGA 
GAGCTGCTGA GCCCAGCCGG AGACCTACTG AAATCTCCCA GTGGTGAACT GAAGACTCCC 
ACTCTAAACC TGTTTGTACC AGAAATACAA GAAGTCAGAG TTAGTCCAAT TGTCTCTAAG 
AAGGGATACC TACACTTCCT GGAGCCACAT ACAGGAGGCT GGGTGAAGAG ATATGTAGTA 
GTCAGGCGAC CCTATGTCTA CATTTACAAT AGCGATCGGG ACCCAGTGGA GAGAGCCATC 
CTTAACCTCT CCCAGGCCCA TGTTGAATAT AGTGAGGACC AACAAGCTAT GCTGAAGACC 
CCCAACACTT TTGCAGTATG CACACAGCAC CGCGGCATCC TGCTGCAGGC CAGCAATGAC 
AAAGACATGC ACGACTGGCT CTACGCCTTT AACCCTCTCC TGGCGGGCTC CATCCGGTCC 
AAACTGTCCA GGCGCAGATC AGCCCAAATG AGAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_012971268.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826722.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X5, mRNA.

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CloneID	OXa30861
Clone ID Related Accession (Same CDS sequence)	XM_012971268.2 , XM_012971268.3
Accession Version	XM_012971268.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
TTCCAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGCAGT TTCTTGATTC TGATACTCCT 
AGGACATTCT ATCACTTTGA GGTGGCCTGG GACAGCTCTA TGCACAACTC TTTGTTGCTG 
AATCGTGTCA CTCCATACAG AGAGAAGATC TACATGACAT TGTCTGCCTA TGTTGAGATG 
GAAAATTGTA CACAGCCTGC TGTGATCACC AAAGACTTCT GCATGGTTTT CTATTCTCGA 
GATGCCAAAC TCCCAGCTTG TCGCTCCATT CGGAACCTGT TTGGAACTGG CAGCTTACGA 
GCCTCTGAAA GTAATCGTGT GACTGGAGTT TATGAGATGA GTCTGTGTCG TGTTGCAGAA 
ACCAGTAGTC CAGGGATGCA GCGGAGACGG AGACGTGTGC TAGACACCTC AGTAGCATAT 
GTACGTGGGG AGGAAAACCT GGCAGGGTGG AGGCCACGGA GTGACAGCCT TATCCTTGAC 
CACCAGTGGG AACTGGAGAA ACTTAGCTTG CTTCAGGAGG TGGAGAAAAC TCGCCATTAC 
CTGCTGCTGA GAGAGAAACT GGAAGCCACA CTCAGGGGAG CCACAGAATC TCTCACCCCT 
TCCCCAATTG AGGAGATGCT GCCCCTTAGC CCAACATGTC AGCCGCTTCA GCTCACAGAG 
GTTGGATCAC CCCGTGCAGA AGAGAACCCC CTAGAAACTG CCAGCGAAAG GCAGAAAGAG 
CTGGCTGCCA AGTGTCTTCG CCTGCTCACC CACACCTTTA ACAGAGAGTA CAGTTACAGT 
CATGTATGTG TGAGTGCCAG CGAGAGTAAG CTTTCTGAAA TGTCAGTTAC ACTTTTACGG 
GACCCCTCAC TGTCTGGCTT AGGGGTCAAC TCCCTTACAC CATCCAGCAC CTGCCCTTCC 
CTAGTAGAAG GACGTTACTG CAGTCCAGAG GCTAGCCCAC AACCTATCTC CCGCGTATTC 
AGCCCTGAGA GAGAGCTGCT GAGCCCAGCC GGAGACCTAC TGAAATCTCC CAGTGGTGAA 
CTGAAGACTC CCACTCTAAA CCTGTTTGTA CCAGAAATAC AAGAAGTCAG AGTTAGTCCA 
ATTGTCTCTA AGAAGGGATA CCTACACTTC CTGGAGCCAC ATACAGGAGG CTGGGTGAAG 
AGATATGTAG TAGTCAGGCG ACCCTATGTC TACATTTACA ATAGCGATCG GGACCCAGTG 
GAGAGAGCCA TCCTTAACCT CTCCCAGGCC CATGTTGAAT ATAGTGAGGA CCAACAAGCT 
ATGCTGAAGA CCCCCAACAC TTTTGCAGTA TGCACACAGC ACCGCGGCAT CCTGCTGCAG 
GCCAGCAATG ACAAAGACAT GCACGACTGG CTCTACGCCT TTAACCCTCT CCTGGCGGGC 
TCCATCCGGT CCAAACTGTC CAGGCGCAGA TCAGCCCAAA TGAGAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826722.1
CDS	222..5312
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDTVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRQFLDSDTPRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_012971268.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971268.2

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
TTCCAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGCAGT TTCTTGATTC TGATACTCCT 
AGGACATTCT ATCACTTTGA GGTGGCCTGG GACAGCTCTA TGCACAACTC TTTGTTGCTG 
AATCGTGTCA CTCCATACAG AGAGAAGATC TACATGACAT TGTCTGCCTA TGTTGAGATG 
GAAAATTGTA CACAGCCTGC TGTGATCACC AAAGACTTCT GCATGGTTTT CTATTCTCGA 
GATGCCAAAC TCCCAGCTTG TCGCTCCATT CGGAACCTGT TTGGAACTGG CAGCTTACGA 
GCCTCTGAAA GTAATCGTGT GACTGGAGTT TATGAGATGA GTCTGTGTCG TGTTGCAGAA 
ACCAGTAGTC CAGGGATGCA GCGGAGACGG AGACGTGTGC TAGACACCTC AGTAGCATAT 
GTACGTGGGG AGGAAAACCT GGCAGGGTGG AGGCCACGGA GTGACAGCCT TATCCTTGAC 
CACCAGTGGG AACTGGAGAA ACTTAGCTTG CTTCAGGAGG TGGAGAAAAC TCGCCATTAC 
CTGCTGCTGA GAGAGAAACT GGAAGCCACA CTCAGGGGAG CCACAGAATC TCTCACCCCT 
TCCCCAATTG AGGAGATGCT GCCCCTTAGC CCAACATGTC AGCCGCTTCA GCTCACAGAG 
GTTGGATCAC CCCGTGCAGA AGAGAACCCC CTAGAAACTG CCAGCGAAAG GCAGAAAGAG 
CTGGCTGCCA AGTGTCTTCG CCTGCTCACC CACACCTTTA ACAGAGAGTA CAGTTACAGT 
CATGTATGTG TGAGTGCCAG CGAGAGTAAG CTTTCTGAAA TGTCAGTTAC ACTTTTACGG 
GACCCCTCAC TGTCTGGCTT AGGGGTCAAC TCCCTTACAC CATCCAGCAC CTGCCCTTCC 
CTAGTAGAAG GACGTTACTG CAGTCCAGAG GCTAGCCCAC AACCTATCTC CCGCGTATTC 
AGCCCTGAGA GAGAGCTGCT GAGCCCAGCC GGAGACCTAC TGAAATCTCC CAGTGGTGAA 
CTGAAGACTC CCACTCTAAA CCTGTTTGTA CCAGAAATAC AAGAAGTCAG AGTTAGTCCA 
ATTGTCTCTA AGAAGGGATA CCTACACTTC CTGGAGCCAC ATACAGGAGG CTGGGTGAAG 
AGATATGTAG TAGTCAGGCG ACCCTATGTC TACATTTACA ATAGCGATCG GGACCCAGTG 
GAGAGAGCCA TCCTTAACCT CTCCCAGGCC CATGTTGAAT ATAGTGAGGA CCAACAAGCT 
ATGCTGAAGA CCCCCAACAC TTTTGCAGTA TGCACACAGC ACCGCGGCAT CCTGCTGCAG 
GCCAGCAATG ACAAAGACAT GCACGACTGG CTCTACGCCT TTAACCCTCT CCTGGCGGGC 
TCCATCCGGT CCAAACTGTC CAGGCGCAGA TCAGCCCAAA TGAGAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1a ( XM_012971269.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826723.1 Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X6, mRNA.

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CloneID	OXa30862
Clone ID Related Accession (Same CDS sequence)	XM_012971269.3 , XM_012971269.2
Accession Version	XM_012971269.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
TTCCAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGACAT TCTATCACTT TGAGGTGGCC 
TGGGACAGCT CTATGCACAA CTCTTTGTTG CTGAATCGTG TCACTCCATA CAGAGAGAAG 
ATCTACATGA CATTGTCTGC CTATGTTGAG ATGGAAAATT GTACACAGCC TGCTGTGATC 
ACCAAAGACT TCTGCATGGT TTTCTATTCT CGAGATGCCA AACTCCCAGC TTGTCGCTCC 
ATTCGGAACC TGTTTGGAAC TGGCAGCTTA CGAGCCTCTG AAAGTAATCG TGTGACTGGA 
GTTTATGAGA TGAGTCTGTG TCGTGTTGCA GAAACCAGTA GTCCAGGGAT GCAGCGGAGA 
CGGAGACGTG TGCTAGACAC CTCAGTAGCA TATGTACGTG GGGAGGAAAA CCTGGCAGGG 
TGGAGGCCAC GGAGTGACAG CCTTATCCTT GACCACCAGT GGGAACTGGA GAAACTTAGC 
TTGCTTCAGG AGGTGGAGAA AACTCGCCAT TACCTGCTGC TGAGAGAGAA ACTGGAAGCC 
ACACTCAGGG GAGCCACAGA ATCTCTCACC CCTTCCCCAA TTGAGGAGAT GCTGCCCCTT 
AGCCCAACAT GTCAGCCGCT TCAGCTCACA GAGGTTGGAT CACCCCGTGC AGAAGAGAAC 
CCCCTAGAAA CTGCCAGCGA AAGGCAGAAA GAGCTGGCTG CCAAGTGTCT TCGCCTGCTC 
ACCCACACCT TTAACAGAGA GTACAGTTAC AGTCATGTAT GTGTGAGTGC CAGCGAGAGT 
AAGCTTTCTG AAATGTCAGT TACACTTTTA CGGGACCCCT CACTGTCTGG CTTAGGGGTC 
AACTCCCTTA CACCATCCAG CACCTGCCCT TCCCTAGTAG AAGGACGTTA CTGCAGTCCA 
GAGGCTAGCC CACAACCTAT CTCCCGCGTA TTCAGCCCTG AGAGAGAGCT GCTGAGCCCA 
GCCGGAGACC TACTGAAATC TCCCAGTGGT GAACTGAAGA CTCCCACTCT AAACCTGTTT 
GTACCAGAAA TACAAGAAGT CAGAGTTAGT CCAATTGTCT CTAAGAAGGG ATACCTACAC 
TTCCTGGAGC CACATACAGG AGGCTGGGTG AAGAGATATG TAGTAGTCAG GCGACCCTAT 
GTCTACATTT ACAATAGCGA TCGGGACCCA GTGGAGAGAG CCATCCTTAA CCTCTCCCAG 
GCCCATGTTG AATATAGTGA GGACCAACAA GCTATGCTGA AGACCCCCAA CACTTTTGCA 
GTATGCACAC AGCACCGCGG CATCCTGCTG CAGGCCAGCA ATGACAAAGA CATGCACGAC 
TGGCTCTACG CCTTTAACCC TCTCCTGGCG GGCTCCATCC GGTCCAAACT GTCCAGGCGC 
AGATCAGCCC AAATGAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826723.1
CDS	222..5285
Translation	MAGASVKVAVRVRPFNSREIGKDSKCIIQMSGNTTTIVNPKQPKETPKSFSFDYSYWSHTSEEDSQYAGQRQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPTQQGIIPQLCEDLFSRISDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYQDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQRRHDADAQTTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGTNKNKKKKKNDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNRLIRELKDEVARLRDLLGAQGLSDIIDTVNALTGMSPSSSMSGLSSRAASVTSLHERLAFTPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMDREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDATMRQDIVLSGHFIRDEHCIFRSDVLACGSVVVTLEPCDGADTYVNGKKLTDPCILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPSEPVDWAFAQRELLEKQGIDMKQEMDQRLQELEDQYRKEREEANYLLEQQRLDYESRLEALQRQMDSRYFTEVNEEEEEPEDEVQWTEQEYDLALWAFRKWKWYQFTSLRDQLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPDAHTEREKRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDMMREMYDRAAELPSGLTEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYSVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFQKFQAETCPGMTRSGTSQEELRIVEGGQGQNPDSCPSADEVNNNTCAASLQSSLLDSPTKPPFDLSDTMGHPLKLGSIFTFRVTVLQASSIPPEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTQSFVEYVKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRSSRRLLPRVMPLSKPVPATKLSRQRVPSVGPCHCKYDLMAYFQICELEANGDYIPVSVDHGVGLPCHGTFLLHQGIQRRISVTLVHEAGAELKWLEVRELVVGRIRNTPEADESLIDPNILSLNILSAGYRPPEGGDRTFYHFEVAWDSSMHNSLLLNRVTPYREKIYMTLSAYVEMENCTQPAVITKDFCMVFYSRDAKLPACRSIRNLFGTGSLRASESNRVTGVYEMSLCRVAETSSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEATLRGATESLTPSPIEEMLPLSPTCQPLQLTEVGSPRAEENPLETASERQKELAAKCLRLLTHTFNREYSYSHVCVSASESKLSEMSVTLLRDPSLSGLGVNSLTPSSTCPSLVEGRYCSPEASPQPISRVFSPERELLSPAGDLLKSPSGELKTPTLNLFVPEIQEVRVSPIVSKKGYLHFLEPHTGGWVKRYVVVRRPYVYIYNSDRDPVERAILNLSQAHVEYSEDQQAMLKTPNTFAVCTQHRGILLQASNDKDMHDWLYAFNPLLAGSIRSKLSRRRSAQMRI

Target ORF information:

RefSeq Version	XM_012971269.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1A (kif1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971269.2

ORF Insert Sequence:

ATGGCGGGAG CGTCGGTGAA GGTTGCGGTC AGAGTGCGGC CGTTCAACTC AAGAGAAATT 
GGGAAGGATT CAAAATGTAT CATCCAGATG TCTGGAAATA CAACCACAAT CGTCAACCCC 
AAACAGCCGA AGGAGACTCC CAAAAGCTTC AGCTTTGACT ATTCCTACTG GTCACACACT 
TCAGAAGAGG ACAGTCAGTA TGCGGGCCAG CGTCAGGTGT ATCGTGACAT TGGAGAAGAG 
ATGCTGCTTC ACGCATTTGA AGGATACAAT GTCTGTATCT TTGCCTATGG ACAGACTGGG 
GCTGGGAAAT CCTACACTAT GATGGGCAGA CAGGAGCCAA CCCAACAAGG CATCATACCC 
CAGCTGTGTG AAGATCTTTT CTCTCGAATC AGTGACACAA CCAATGACAA CATGTCTTAC 
TCTGTAGAGG TCAGTTATAT GGAAATATAC TGTGAACGAG TTAGAGACCT GCTGAACCCC 
AAAAACAAGG GGAACCTGCG AGTGCGTGAA CACCCCCTTC TGGGACCCTA TGTAGAAGAT 
CTGTCGAAGC TAGCCGTCAC TTCTTACCAG GATATTCAGG ATCTCATGGA CTCTGGTAAC 
AAGGCCAGGA CTGTTGCTGC TACCAACATG AATGAAACTA GCAGTCGTTC CCATGCTGTG 
TTCAACATCA TCTTTACCCA GCGCAGACAT GATGCTGATG CTCAGACCAC AACAGAGAAG 
GTCAGTAAAA TAAGTCTGGT AGATCTTGCT GGAAGTGAAA GAGCTGACTC TACAGGTGCA 
AAGGGAACAA GATTAAAGGA AGGTGCAAAT ATCAATAAGT CTCTCACTAC CCTTGGAAAA 
GTGATATCTG CTCTTGCTGA AATGGACTCT GGGACTAATA AGAACAAAAA GAAGAAGAAA 
AATGATTTCA TTCCCTACCG AGATTCAGTG CTTACTTGGC TCCTGAGAGA GAATTTAGGT 
GGGAACTCTC GCACAGCCAT GGTTGCTGCA CTGAGCCCAG CAGATATTAA TTATGATGAA 
ACCCTCAGCA CCTTGAGATA TGCTGACCGT GCCAAACAGA TTAGATGCAA TGCTGTTATA 
AATGAGGATC CAAATAACCG TCTGATCCGT GAGCTGAAAG ATGAGGTGGC ACGACTAAGG 
GACCTCTTGG GAGCACAAGG ATTGTCTGAC ATTATTGACA CCGTTAATGC CCTGACTGGT 
ATGAGTCCTT CGTCTTCCAT GTCTGGTTTG TCCAGCCGAG CTGCATCTGT CACCAGCTTA 
CACGAGCGCC TGGCATTCAC ACCTGGCAGT GAAGAAGCCA TTGAGAGACT CAAGGAGACC 
GAAAAAATTA TAGCAGAATT GAATGAGACA TGGGAGGAAA AACTGCGCAG GACTGAAGCT 
ATTCGCATGG ACAGAGAAGC TTTGCTGGCA GAGATGGGAG TAGCTATGAG AGAAGATGGA 
GGAACACTTG GAGTCTTTTC ACCTAAAAAG ACTCCTCACC TTGTAAATCT TAATGAAGAT 
CCTCTAATGT CTGAGTGTCT ATTATATTAC ATCAAAGATG GCATTACCAG GGTTGGGCGT 
GAGGATGCAA CAATGAGACA AGACATTGTG CTGAGTGGCC ACTTTATTCG TGATGAGCAT 
TGTATTTTCC GGAGTGATGT ATTGGCTTGC GGATCAGTGG TGGTAACTCT TGAGCCCTGT 
GATGGAGCTG ACACATATGT AAATGGCAAG AAGCTGACAG ATCCTTGTAT TCTGAGATCC 
GGAAACAGAA TTATTATGGG CAAGAGTCAC GTATTCCGCT TCAACCACCC TGAGCAAGCG 
CGCCAGGAAA GGGAACGCAC GCCCTGTGCC GAAACCCCAT CTGAACCTGT GGACTGGGCA 
TTTGCTCAGA GAGAGCTCCT GGAGAAGCAA GGAATTGATA TGAAACAAGA GATGGATCAG 
AGGCTCCAAG AACTGGAAGA TCAATACAGA AAAGAACGTG AAGAAGCAAA TTACCTTCTG 
GAACAGCAGC GACTGGACTA TGAAAGCAGA CTGGAAGCAT TACAGAGGCA GATGGATTCT 
AGATATTTTA CCGAAGTGAA CGAGGAAGAA GAAGAACCTG AAGATGAAGT TCAGTGGACT 
GAACAGGAAT ATGATCTGGC ACTCTGGGCT TTTCGGAAGT GGAAGTGGTA CCAGTTTACA 
TCACTCCGAG ATCAACTTTG GGGAAATGCC ATCTTCCTTA AAGAAGCCAA TGCTATCAGT 
GTGGAATTGA AGAAAAAGGT ACAGTTCCAG TTTGTTCTGC TGACAGACAC TTTGTATTCT 
CCTCTCCCTC CGGACCTGCT GCCGCCTGAT GCCCATACTG AACGAGAGAA ACGTCCTTTC 
CCAAGAACCA TTGTAGCTGT GGAGGTTCAG GATCAGAAAA ATGGTGCAAC TCACTACTGG 
ACCTTAGAAA AACTCAGGCA ACGTCTGGAC ATGATGAGGG AGATGTATGA CCGGGCTGCA 
GAGCTTCCTT CTGGTCTGAC TGAGGACTGT GATAATGTGG TGACTGGAGG AGATCCATTT 
TATGACCGAT TCCCTTGGTT CCGACTGGTG GGCAGGGCTT TTGTGTACCT GAGTAATTTG 
CTGTACTCTG TCCCTTTGGT TCATCGAGTC GCCATTGTCA GTGAGAAAGG AGAAGTGAAA 
GGATTTCTTC GTGTTGCTGT TCAGGCCATA TCAGCTGACG AGGAAGCTCC TGATTATGGT 
TCTGGAGTCC GGCAGTCTGG CACAGCTAAA ATATCCTTTG ATGATCAACA TTTTCAAAAG 
TTCCAGGCTG AAACCTGCCC AGGAATGACA CGCTCAGGCA CTTCACAAGA AGAGCTTCGA 
ATTGTTGAGG GAGGACAAGG ACAAAACCCG GACTCGTGTC CTTCTGCTGA CGAAGTGAAC 
AACAATACCT GTGCAGCTTC TCTGCAGTCT TCCTTGTTGG ATAGTCCTAC CAAGCCACCC 
TTTGACCTCT CAGACACTAT GGGCCACCCT CTGAAACTGG GCAGCATCTT CACATTCAGA 
GTGACTGTCT TGCAGGCATC GAGTATACCT CCAGAATATG CTGACATATT CTGCCAATTC 
AATTTTATTC ATCGCCATGA TGAGGCCTTT TCAACTGAAC CATTAAAAAA CACAGGCCGA 
GGGCCACCAC TGGGCTTCTA CCATGTGCAA AATATTGCTG TGGAGGTCAC TCAGTCATTT 
GTGGAATATG TGAAGAGTCA GCCAATTGTC TTTGAGGTGT TTGGACATTA TCAGCAGCAT 
CCTTTCCCTC CTCTGTGCAA AGATGTTCTA AGCCCTCTGC GCTCTTCCAG ACGCCTGCTT 
CCAAGGGTCA TGCCTCTGTC TAAACCAGTT CCTGCCACTA AGTTGAGCAG ACAGCGAGTT 
CCCAGTGTTG GTCCCTGTCA CTGCAAGTAT GACCTGATGG CTTACTTCCA GATCTGTGAA 
CTTGAAGCCA ATGGAGATTA CATACCTGTG TCAGTAGATC ATGGAGTGGG TTTGCCTTGT 
CATGGGACAT TTCTTCTCCA CCAGGGTATC CAGAGACGCA TTTCAGTGAC ACTGGTGCAT 
GAAGCTGGCG CTGAGCTGAA GTGGCTGGAA GTGCGAGAGC TGGTTGTAGG TCGCATCCGG 
AATACCCCAG AGGCAGATGA GTCTCTAATT GACCCAAACA TCTTGTCACT GAATATTCTA 
TCTGCGGGAT ATCGGCCCCC TGAAGGAGGT GATCGGACAT TCTATCACTT TGAGGTGGCC 
TGGGACAGCT CTATGCACAA CTCTTTGTTG CTGAATCGTG TCACTCCATA CAGAGAGAAG 
ATCTACATGA CATTGTCTGC CTATGTTGAG ATGGAAAATT GTACACAGCC TGCTGTGATC 
ACCAAAGACT TCTGCATGGT TTTCTATTCT CGAGATGCCA AACTCCCAGC TTGTCGCTCC 
ATTCGGAACC TGTTTGGAAC TGGCAGCTTA CGAGCCTCTG AAAGTAATCG TGTGACTGGA 
GTTTATGAGA TGAGTCTGTG TCGTGTTGCA GAAACCAGTA GTCCAGGGAT GCAGCGGAGA 
CGGAGACGTG TGCTAGACAC CTCAGTAGCA TATGTACGTG GGGAGGAAAA CCTGGCAGGG 
TGGAGGCCAC GGAGTGACAG CCTTATCCTT GACCACCAGT GGGAACTGGA GAAACTTAGC 
TTGCTTCAGG AGGTGGAGAA AACTCGCCAT TACCTGCTGC TGAGAGAGAA ACTGGAAGCC 
ACACTCAGGG GAGCCACAGA ATCTCTCACC CCTTCCCCAA TTGAGGAGAT GCTGCCCCTT 
AGCCCAACAT GTCAGCCGCT TCAGCTCACA GAGGTTGGAT CACCCCGTGC AGAAGAGAAC 
CCCCTAGAAA CTGCCAGCGA AAGGCAGAAA GAGCTGGCTG CCAAGTGTCT TCGCCTGCTC 
ACCCACACCT TTAACAGAGA GTACAGTTAC AGTCATGTAT GTGTGAGTGC CAGCGAGAGT 
AAGCTTTCTG AAATGTCAGT TACACTTTTA CGGGACCCCT CACTGTCTGG CTTAGGGGTC 
AACTCCCTTA CACCATCCAG CACCTGCCCT TCCCTAGTAG AAGGACGTTA CTGCAGTCCA 
GAGGCTAGCC CACAACCTAT CTCCCGCGTA TTCAGCCCTG AGAGAGAGCT GCTGAGCCCA 
GCCGGAGACC TACTGAAATC TCCCAGTGGT GAACTGAAGA CTCCCACTCT AAACCTGTTT 
GTACCAGAAA TACAAGAAGT CAGAGTTAGT CCAATTGTCT CTAAGAAGGG ATACCTACAC 
TTCCTGGAGC CACATACAGG AGGCTGGGTG AAGAGATATG TAGTAGTCAG GCGACCCTAT 
GTCTACATTT ACAATAGCGA TCGGGACCCA GTGGAGAGAG CCATCCTTAA CCTCTCCCAG 
GCCCATGTTG AATATAGTGA GGACCAACAA GCTATGCTGA AGACCCCCAA CACTTTTGCA 
GTATGCACAC AGCACCGCGG CATCCTGCTG CAGGCCAGCA ATGACAAAGA CATGCACGAC 
TGGCTCTACG CCTTTAACCC TCTCCTGGCG GGCTCCATCC GGTCCAAACT GTCCAGGCGC 
AGATCAGCCC AAATGAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	kif1a
Entrez Gene ID	100127546
Full Name	kinesin family member 1A
General protein information	Preferred Names kinesin family member 1A Names kinesin-like protein KIF1A kinesin 1a
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	KIF1A	XP_002799156.1
Gallus gallus (chicken)	KIF1A	XP_003641781.1
Rattus norvegicus (Norway rat)	Kif1a	XP_006245623.1
Drosophila melanogaster (fruit fly)	unc-104	NP_001246373.1
Anopheles gambiae (African malaria mosquito)	KIF1A_ANOGA	XP_314493.3
Pan troglodytes (chimpanzee)	KIF1A	XP_003309612.1
Bos taurus (cattle)	KIF1A	XP_005205116.1
Mus musculus (house mouse)	Kif1a	NP_032466.2
Caenorhabditis elegans (roundworm)	unc-104	NP_001022041.1
Homo sapiens (human)	KIF1A	NP_004312.2
Canis lupus familiaris (dog)	KIF1A	NP_001161945.1
Danio rerio (zebrafish)	kif1a	XP_005166002.1
Danio rerio (zebrafish)	kif1ab	XP_005171271.1
Xenopus tropicalis (tropical clawed frog)	kif1a	XP_002933380.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

kif1a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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