pc.2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following pc.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pc.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa30697 XM_012962238.3
Latest version!
Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa30697 XM_012962236.3
Latest version!
Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa30697 XM_012962235.3
Latest version!
Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa30697 XM_012962237.3
Latest version!
Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa30697 XM_012962239.1
Latest version!
Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OXa30697 XM_012962236.2 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa30697 XM_012962235.2 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa30697 XM_012962237.2 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa30697 XM_012962238.2 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962237.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962237.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817691.1
    CDS151..3684
    Translation

    Target ORF information:

    RefSeq Version XM_012962237.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962237.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962236.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962236.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817690.1
    CDS98..3631
    Translation

    Target ORF information:

    RefSeq Version XM_012962236.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962236.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962235.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962235.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817689.1
    CDS109..3642
    Translation

    Target ORF information:

    RefSeq Version XM_012962235.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962235.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962238.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962238.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817692.1
    CDS20..3553
    Translation

    Target ORF information:

    RefSeq Version XM_012962238.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962238.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962236.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817690.1
    CDS123..3656
    Translation

    Target ORF information:

    RefSeq Version XM_012962236.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962236.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962235.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962235.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817689.1
    CDS106..3639
    Translation

    Target ORF information:

    RefSeq Version XM_012962235.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962235.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962239.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817693.1
    CDS20..3553
    Translation

    Target ORF information:

    RefSeq Version XM_012962239.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962237.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817691.1
    CDS151..3684
    Translation

    Target ORF information:

    RefSeq Version XM_012962237.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962237.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962238.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817692.1
    CDS54..3587
    Translation

    Target ORF information:

    RefSeq Version XM_012962238.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962238.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.