ORF » Species Summary » Xenopus tropicalis » pc.2 cDNA ORF clone

pc.2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following pc.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pc.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa30697 XM_012962238.3
Latest version!		 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa30697 XM_012962236.3
Latest version!		 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa30697 XM_012962235.3
Latest version!		 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa30697 XM_012962237.3
Latest version!		 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa30697 XM_012962239.1
Latest version!		 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OXa30697 XM_012962236.2 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa30697 XM_012962235.2 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa30697 XM_012962237.2 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa30697 XM_012962238.2 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    pc.2 ( XM_012962237.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817691.1 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962237.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962237.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
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    121
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    301
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    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817691.1
    CDS151..3684
    Translation

    Target ORF information:

    RefSeq Version XM_012962237.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012962237.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pc.2 ( XM_012962236.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817690.1 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962236.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962236.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817690.1
    CDS98..3631
    Translation

    Target ORF information:

    RefSeq Version XM_012962236.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012962236.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pc.2 ( XM_012962235.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817689.1 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962235.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962235.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817689.1
    CDS109..3642
    Translation

    Target ORF information:

    RefSeq Version XM_012962235.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012962235.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pc.2 ( XM_012962238.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817692.1 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962238.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962238.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817692.1
    CDS20..3553
    Translation

    Target ORF information:

    RefSeq Version XM_012962238.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012962238.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pc.2 ( XM_012962236.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817690.1 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962236.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817690.1
    CDS123..3656
    Translation

    Target ORF information:

    RefSeq Version XM_012962236.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012962236.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pc.2 ( XM_012962235.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817689.1 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962235.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962235.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817689.1
    CDS106..3639
    Translation

    Target ORF information:

    RefSeq Version XM_012962235.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012962235.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pc.2 ( XM_012962239.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817693.1 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962239.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817693.1
    CDS20..3553
    Translation

    Target ORF information:

    RefSeq Version XM_012962239.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012962239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pc.2 ( XM_012962237.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817691.1 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962237.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817691.1
    CDS151..3684
    Translation

    Target ORF information:

    RefSeq Version XM_012962237.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012962237.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pc.2 ( XM_012962238.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817692.1 Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OXa30697
    Clone ID Related Accession (Same CDS sequence) XM_012962236.2 , XM_012962235.2 , XM_012962237.3 , XM_012962239.1 , XM_012962236.3 , XM_012962235.3 , XM_012962238.3 , XM_012962237.2 , XM_012962238.2
    Accession Version XM_012962238.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pyruvate carboxylase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817692.1
    CDS54..3587
    Translation

    Target ORF information:

    RefSeq Version XM_012962238.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pyruvate carboxylase, gene 2 (pc.2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012962238.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGCAGC TACGTTTTGT CAAGGGAGGC CTGACCTTCC TTGGTGTGAA GCGCATCTCT 
    GTTCTCAATG CTGCCTTTCA GAGCACCCAA CGTGTGGATT ACAAGCCTAT CAAAAAAGTT 
    TTAGTGGCCA ACAGAGGTGA GATTGCCACA CGTGTCTTTA GAGCATGCAC AGAACTTGGG 
    ATAAGGACTG TAGCTGTCTA CTCCGAGCAG GACACAGGCC AAATGCATAG ACAAAAAGCA 
    GATGAGGCTT ATATCATTGG AAAAGGATTG CCGCCAGTCC AAGCCTACTT AAACATACCA 
    GATATCATCA AGGTTGCTAA GGAGAATGAA GTTGATGCAG TTCATCCTGG TTATGGTTTC 
    CTTTCTGAGC GCTCTGATTT TGCTCAGGCC TGCACAGATG CAGGTGTAAG ATTCATTGGA 
    CCCTCCCCTG AGGTGGTACG CAAGATGGGT GACAAGGTAG AGGCCAGAGC CATTGCAATT 
    AGCGCAGGAG TACCGGTTGT TCCAGGCACA GACTCTCCAA TCAGCTGCCT GACAGAGGCC 
    AAGGAATTCT CCAACAAATA TGGCTTTCCT ATAATATTCA AGGCTGCATA TGGAGGTGGT 
    GGACGTGGTA TGAGAGTAGT GCGGAGCTAT GAGGAGCTGG AGGAAAATTA CACAAGAGCC 
    TACTCTGAAG CCCTTTCTGC CTTTGGAAAT GGAGCATTGT TTGTGGAAAA ATTCATTGAA 
    AAACCTCGTC ATATTGAAGT TCAGATACTT GGTGATAAAT TTGGAAATGT GATACATTTG 
    TATGAAAGAG ACTGTTCCAT CCAGAGAAGG CACCAGAAGG TGGTTGAAAT AGCCCCAGCT 
    GCACAGCTGG ATCCTCAGCT CAGAGACAGA CTTACGCATG ACTCAGTCAA ACTGGCAAAA 
    CAGGTGGGAT ATGAGAATGC CGGCACTGTG GAGTTCTTGG TGGATAAACA TGGCAAGCAT 
    TATTTCATAG AAGTCAACTC CAGGCTTCAA GTTGAACATA CAGTAACAGA AGAAATTACA 
    GACGTTGACC TTGTCCATGC CCAGATCCAT ATTGCAGAGG GAAAAAGCCT TCCTGAGCTT 
    GGCCTAAAGC AAGAGAACAT CCGTATCAAT GGTTGTGCCA TTCAGTGCAG AGTCACAACA 
    GAAGATCCTT CCCGTGGGTT TCAGCCTGAT ACTGGTCGGA TTGAGGTTTT CCGTAGTGGA 
    GAGGGAATGG GAATCCGACT GGACAGCGCT TCAGCTTTTC AAGGAGCTGT CATTTCCCCA 
    CATTATGACT CTCTTCTGGT CAAGGTCATT GCACATGGGA AAGACCAACA AGTTGCAGCC 
    ACAAAAATGA GCAGAGCCTT GGCAGAGTTC CGCATTAGGG GTGTTAAGAC CAACATTCCC 
    TTCTTGCAAA ATGTCTTGAA CAATAACCAG TTTCTCCATG GTACAGTAGA CACACAGTTT 
    ATTGATGAAA ATCCAGACCT CTTCAATTTG AAGCCTGTGC AAAACAGAGC TCAAAAACTG 
    CTACATTATC TAGGTCATGT TATGGTAAAT GGGCCAACAA CACCAATACC AGTTAAAGCA 
    AAACCATCCT CTGTAGACCC TGTTGTACCT CCAATCCCAA TGAATGATCC ACCTCCTGGC 
    TTTCGTGATA TTCTCCTGCG AGAAGGTCCG GAAGGTTTTG CTAAAGCGAT ACGACAGCAT 
    AAAGGCTTAT TACTTATGGA TACAACCTTC AGAGATGCCC ACCAGTCTCT GCTTGCAACC 
    CGTGTACGCA CACATGACCT TAAGAATATT GCTCCCTTTG TGTCGCATAA CTTCAACAAC 
    CTTTTCAGCT TAGAGAATTG GGGAGGTGCC ACATTTGATG TTGCAATGCG CTTCCTTTAT 
    GAATGCCCTT GGAGACGCCT ACAAGAATTA AGGGAACTCA TTCCGAACAT CCCCTTCCAA 
    ATGCTACTTA GAGGAGCAAA TGCCGTAGGT TATACTAACT ACCCAGATAA TGCAGTCTTC 
    AAATTTTGTG AGGTGGCAAA GGAAAATGGA ATGGATATCT TTAGGGTCTT TGACTCTCTG 
    AACTACCTGC CTAACATGAT CTTGGGCATG GAGGCAGCCG GTCGGGCAGG TGGAGTGGTG 
    GAAGCTGCTA TCTCTTACAC GGGTGATGTG GCTGATCCAA CCCGCACTAA ATATACTCTG 
    GACTACTATG TGAACCTGGC TGAGGAGCTG GTTAAAGCTG GAACCCACAT ATTGTGTATC 
    AAGGATATGG CTGGACTTCT GAAACCCAAA TCTTCAGACA TGCTGATCCG TGCACTGCGA 
    GACAGGTTTC CCAATATTCC CATCCATGTG CACACGCATG ACACTGCTGG AGCTGGCGTG 
    GCCTCCATGT TGGCATGTGC ACACGCAGGA GCTGACATTG TAGACGTGGC AGTTGATTCT 
    ATGTCTGGGA TGACCTCACA GCCGAGCATG GGGGCACTTG TGGCATGCGC TAAGGGCACA 
    GACCAGGACA CAGGTATTCA GCTTGAAAAA GTGTTCGATT ACAGTGAATA CTGGGAAGTA 
    GCCCGCGGTC TTTATTCGGC TTTTGACTGC ACTGCAACCA TGAAATCTGG CAATGCTGAT 
    GTGTATGAAA ATGAAATCCC TGGTGGCCAG TATACCAACC TTCACTTCCA GGCCCACAGC 
    ATGGGACTAG GCAACAAATT CAAGGAAGTG AAGAAGGCAT ATGCTGAAGC CAATAAAATC 
    CTTGGGGATG TCATTAAGGT TACACCTTCC TCTAAGATTG TCGGAGACCT GGCGCAGTTT 
    ATGGTGCATA ATGGCCTAAA CCGTGAACAA GTGGAAACAA TGGCTGATGA ACTCTCCTTC 
    CCCCTCTCTG TGGTGGAATA TATGCAAGGC TATGTTGGAA TCCCTTATGG AGGATTTCCG 
    GAGCCTTTCC GTTCTAAGGT TCTTAAAGAT CTGCCACGCA TTGAGGGCAG GCCTGGGGCA 
    TCACTCCCAT CAATGGATTT TACAAAACTG GAGGAGAAAC TGAAAAGCAA ATATGATGAC 
    ATTACCCCAG AAGATATTAT GTCCGCTGCA ATGTACCCAA AAGTGTTCGA GGAGTACAAG 
    GATTTCGGCT CTCAGTTTGG GCCAGTAGAA TGTCTCAACA CCCGTCTCTT TTTGGAAGGA 
    CCTAAGATTG CAGAAGAATT TGAGGTGGAA TTAGAAAGAG GAAAGACCTT GCATATCAAA 
    GCTCTTGCCC TGGGAGATCT TAATAAAGCT GGTCAGAGGG AAGTCTTCTT TGAACTCAAT 
    GGTCAACTCA GATCTGTCCT TGTCAAAGAC ACTCAGGCCA TGAAGGAGAT GCACTTCCAC 
    CCCAAAGCCC TGAAAGATGT GAAAGGTCAG ATAGGTGCCC CAATGCCAGG CAAGGTCATT 
    GACATCAAGG TGAAGGAGGG GACCAAAGTT GAAAAGGGAC AGCCGCTCTG CGTGCTCAGC 
    GCCATGAAGA TGGAGACACT GGTCAACTCT CCGGTTACTG GAACAGTCAA GAAAGTCTAC 
    GTAAAAACCG ATATGCATCT GGAAGGCGAG GACCTTTTAC TGGAAATTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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