plcb4 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_002931736.4	XP_002931782.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_031902882.1	XP_031758742.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_031902884.1	XP_031758744.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_031902885.1	XP_031758745.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
XM_012963848.2	XP_012819302.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012963849.2	XP_012819303.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012963850.2	XP_012819304.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_018094150.1	XP_017949639.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
XM_002931736.5	XP_002931782.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012963849.3	XP_012819303.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012963847.2	XP_012819301.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_018094149.1	XP_017949638.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012963848.3	XP_012819302.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_031902883.1	XP_031758743.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_018094149.2	XP_017949638.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa44584	XM_018094150.1 Latest version!	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa10810	XM_012963849.3 Latest version!	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa10810	XM_018094149.2 Latest version!	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa10810	XM_031902883.1 Latest version!	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa10810	XM_002931736.5 Latest version!	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa10810	XM_012963848.3 Latest version!	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa10810	XM_031902882.1 Latest version!	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa10812	XM_031902884.1 Latest version!	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59197	XM_031902885.1 Latest version!	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa10810	XM_012963847.2 Latest version!	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa10812	XM_012963850.2 Latest version!	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa10810	XM_002931736.4	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa10810	XM_012963849.2	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa10810	XM_012963848.2	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa10810	XM_018094149.1	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

plcb4 ( XM_018094150.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949639.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa44584
Clone ID Related Accession (Same CDS sequence)	XM_018094150.1
Accession Version	XM_018094150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949639.1
CDS	66..3062
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKG

Target ORF information:

RefSeq Version	XM_018094150.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094150.1

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_031902882.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758742.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa10810
Clone ID Related Accession (Same CDS sequence)	XM_002931736.4 , XM_031902882.1 , XM_012963848.2 , XM_012963849.2 , XM_002931736.5 , XM_012963849.3 , XM_012963847.2 , XM_018094149.1 , XM_012963848.3 , XM_031902883.1 , XM_018094149.2
Accession Version	XM_031902882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758742.1
CDS	262..3879
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_031902882.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902882.1

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_002931736.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931782.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X1, mRNA.

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CloneID	OXa10810
Clone ID Related Accession (Same CDS sequence)	XM_002931736.4 , XM_031902882.1 , XM_012963848.2 , XM_012963849.2 , XM_002931736.5 , XM_012963849.3 , XM_012963847.2 , XM_018094149.1 , XM_012963848.3 , XM_031902883.1 , XM_018094149.2
Accession Version	XM_002931736.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931736.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931782.1
CDS	361..3978
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_002931736.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931736.5

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_012963849.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819303.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa10810
Clone ID Related Accession (Same CDS sequence)	XM_002931736.4 , XM_031902882.1 , XM_012963848.2 , XM_012963849.2 , XM_002931736.5 , XM_012963849.3 , XM_012963847.2 , XM_018094149.1 , XM_012963848.3 , XM_031902883.1 , XM_018094149.2
Accession Version	XM_012963849.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963849.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819303.1
CDS	304..3921
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_012963849.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963849.3

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_012963848.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819302.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10810
Clone ID Related Accession (Same CDS sequence)	XM_002931736.4 , XM_031902882.1 , XM_012963848.2 , XM_012963849.2 , XM_002931736.5 , XM_012963849.3 , XM_012963847.2 , XM_018094149.1 , XM_012963848.3 , XM_031902883.1 , XM_018094149.2
Accession Version	XM_012963848.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963848.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819302.1
CDS	533..4150
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_012963848.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963848.3

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_031902883.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758743.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X5, mRNA.

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CloneID	OXa10810
Clone ID Related Accession (Same CDS sequence)	XM_002931736.4 , XM_031902882.1 , XM_012963848.2 , XM_012963849.2 , XM_002931736.5 , XM_012963849.3 , XM_012963847.2 , XM_018094149.1 , XM_012963848.3 , XM_031902883.1 , XM_018094149.2
Accession Version	XM_031902883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758743.1
CDS	474..4091
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_031902883.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902883.1

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_018094149.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949638.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X3, mRNA.

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CloneID	OXa10810
Clone ID Related Accession (Same CDS sequence)	XM_002931736.4 , XM_031902882.1 , XM_012963848.2 , XM_012963849.2 , XM_002931736.5 , XM_012963849.3 , XM_012963847.2 , XM_018094149.1 , XM_012963848.3 , XM_031902883.1 , XM_018094149.2
Accession Version	XM_018094149.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949638.1
CDS	434..4051
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_018094149.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094149.2

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_031902884.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758744.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X7, mRNA.

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CloneID	OXa10812
Clone ID Related Accession (Same CDS sequence)	XM_031902884.1 , XM_012963850.2
Accession Version	XM_031902884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGTG 
AAAGATATTG TGGCTCAGCA TACAAAAGAG TTCACAGAAA TGGTGAAAGC ACAGAACGCG 
GAAGAGCAAA CACTCCGAGA TGTCCATTTA AGCCAACAGT GTGAGCTCCT GAAAGAGTTG 
CTGCGGGGGA TTCATGCACA ACAAATGCAG CAACTCAAGC TGACCCATGA CAGAGAAAGC 
AAGGAGATGA AGGCCAACCA AGCAAAATTG TCCATGGAAA ACTGCAAAGC CATAAGCCAG 
GATAAATCAA TCAAAAACAA GGCTGAGAGA GAAAGGCGAG TGAGGGAACT GAATAGCAGC 
AACACAAAGA AGTTCCTTGA GGAACGGAAA AGGCTGGCAA TGAAACAGTC GAAAGACGTT 
GATCAGCTGT TAAAAAACCA ACAAGAACAC ATGGAGAACC TGGAGAAGCA GAATGAACAG 
CATTTGAAAG CGTGCCATGC AGTGTCTCAA TCACAAGGCA AAGGAGATAC AGCAGATGGT 
GAAGCTGGAA GCAGAGATGG ACCACCGTCC AGCAACTGTT GTGTAAAGCT GCAAAATGCA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758744.1
CDS	18..3443
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_031902884.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902884.1

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGTG 
AAAGATATTG TGGCTCAGCA TACAAAAGAG TTCACAGAAA TGGTGAAAGC ACAGAACGCG 
GAAGAGCAAA CACTCCGAGA TGTCCATTTA AGCCAACAGT GTGAGCTCCT GAAAGAGTTG 
CTGCGGGGGA TTCATGCACA ACAAATGCAG CAACTCAAGC TGACCCATGA CAGAGAAAGC 
AAGGAGATGA AGGCCAACCA AGCAAAATTG TCCATGGAAA ACTGCAAAGC CATAAGCCAG 
GATAAATCAA TCAAAAACAA GGCTGAGAGA GAAAGGCGAG TGAGGGAACT GAATAGCAGC 
AACACAAAGA AGTTCCTTGA GGAACGGAAA AGGCTGGCAA TGAAACAGTC GAAAGACGTT 
GATCAGCTGT TAAAAAACCA ACAAGAACAC ATGGAGAACC TGGAGAAGCA GAATGAACAG 
CATTTGAAAG CGTGCCATGC AGTGTCTCAA TCACAAGGCA AAGGAGATAC AGCAGATGGT 
GAAGCTGGAA GCAGAGATGG ACCACCGTCC AGCAACTGTT GTGTAAAGCT GCAAAATGCA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_031902885.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758745.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X8, mRNA.

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CloneID	OXa59197
Clone ID Related Accession (Same CDS sequence)	XM_031902885.1
Accession Version	XM_031902885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGGCAAAGGA GATACAGCAG ATGGTGAAGC TGGAAGCAGA GATGGACCAC 
CGTCCAGCAA CTGTTGTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758745.1
CDS	18..3578
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQAKEIQQMVKLEAEMDHRPATVV

Target ORF information:

RefSeq Version	XM_031902885.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902885.1

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGGCAAAGGA GATACAGCAG ATGGTGAAGC TGGAAGCAGA GATGGACCAC 
CGTCCAGCAA CTGTTGTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_002931736.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931782.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10810
Clone ID Related Accession (Same CDS sequence)	XM_002931736.4 , XM_031902882.1 , XM_012963848.2 , XM_012963849.2 , XM_002931736.5 , XM_012963849.3 , XM_012963847.2 , XM_018094149.1 , XM_012963848.3 , XM_031902883.1 , XM_018094149.2
Accession Version	XM_002931736.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931736.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931782.1
CDS	406..4023
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_002931736.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931736.4

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_012963848.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819302.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X3, mRNA.

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CloneID	OXa10810
Clone ID Related Accession (Same CDS sequence)	XM_002931736.4 , XM_031902882.1 , XM_012963848.2 , XM_012963849.2 , XM_002931736.5 , XM_012963849.3 , XM_012963847.2 , XM_018094149.1 , XM_012963848.3 , XM_031902883.1 , XM_018094149.2
Accession Version	XM_012963848.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819302.1
CDS	578..4195
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_012963848.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963848.2

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_012963849.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819303.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa10810
Clone ID Related Accession (Same CDS sequence)	XM_002931736.4 , XM_031902882.1 , XM_012963848.2 , XM_012963849.2 , XM_002931736.5 , XM_012963849.3 , XM_012963847.2 , XM_018094149.1 , XM_012963848.3 , XM_031902883.1 , XM_018094149.2
Accession Version	XM_012963849.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819303.1
CDS	349..3966
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_012963849.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963849.2

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_012963847.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819301.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10810
Clone ID Related Accession (Same CDS sequence)	XM_002931736.4 , XM_031902882.1 , XM_012963848.2 , XM_012963849.2 , XM_002931736.5 , XM_012963849.3 , XM_012963847.2 , XM_018094149.1 , XM_012963848.3 , XM_031902883.1 , XM_018094149.2
Accession Version	XM_012963847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963847.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819301.1
CDS	49..3666
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_012963847.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963847.2

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_018094149.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949638.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X4, mRNA.

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CloneID	OXa10810
Clone ID Related Accession (Same CDS sequence)	XM_002931736.4 , XM_031902882.1 , XM_012963848.2 , XM_012963849.2 , XM_002931736.5 , XM_012963849.3 , XM_012963847.2 , XM_018094149.1 , XM_012963848.3 , XM_031902883.1 , XM_018094149.2
Accession Version	XM_018094149.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949638.1
CDS	1047..4664
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKEHSVVQKLQCTQVDKIVAQFDKEKVSHERTLEKSIKKKGASNCHEMKKETENKIQVLTSDHKSKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_018094149.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094149.1

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGAG 
CACAGCGTTG TGCAGAAGTT ACAGTGCACA CAAGTTGACA AAATAGTGGC ACAGTTTGAC 
AAAGAAAAAG TATCTCATGA GAGGACCTTA GAGAAATCAA TCAAGAAGAA AGGAGCAAGT 
AACTGTCATG AAATGAAGAA AGAAACAGAA AATAAAATTC AGGTTTTAAC TTCAGATCAT 
AAATCCAAGG TGAAAGATAT TGTGGCTCAG CATACAAAAG AGTTCACAGA AATGGTGAAA 
GCACAGAACG CGGAAGAGCA AACACTCCGA GATGTCCATT TAAGCCAACA GTGTGAGCTC 
CTGAAAGAGT TGCTGCGGGG GATTCATGCA CAACAAATGC AGCAACTCAA GCTGACCCAT 
GACAGAGAAA GCAAGGAGAT GAAGGCCAAC CAAGCAAAAT TGTCCATGGA AAACTGCAAA 
GCCATAAGCC AGGATAAATC AATCAAAAAC AAGGCTGAGA GAGAAAGGCG AGTGAGGGAA 
CTGAATAGCA GCAACACAAA GAAGTTCCTT GAGGAACGGA AAAGGCTGGC AATGAAACAG 
TCGAAAGACG TTGATCAGCT GTTAAAAAAC CAACAAGAAC ACATGGAGAA CCTGGAGAAG 
CAGAATGAAC AGCATTTGAA AGCGTGCCAT GCAGTGTCTC AATCACAAGG CAAAGGAGAT 
ACAGCAGATG GTGAAGCTGG AAGCAGAGAT GGACCACCGT CCAGCAACTG TTGTGTAAAG 
CTGCAAAATG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb4 ( XM_012963850.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819304.1 Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X6, mRNA.

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CloneID	OXa10812
Clone ID Related Accession (Same CDS sequence)	XM_031902884.1 , XM_012963850.2
Accession Version	XM_012963850.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGTG 
AAAGATATTG TGGCTCAGCA TACAAAAGAG TTCACAGAAA TGGTGAAAGC ACAGAACGCG 
GAAGAGCAAA CACTCCGAGA TGTCCATTTA AGCCAACAGT GTGAGCTCCT GAAAGAGTTG 
CTGCGGGGGA TTCATGCACA ACAAATGCAG CAACTCAAGC TGACCCATGA CAGAGAAAGC 
AAGGAGATGA AGGCCAACCA AGCAAAATTG TCCATGGAAA ACTGCAAAGC CATAAGCCAG 
GATAAATCAA TCAAAAACAA GGCTGAGAGA GAAAGGCGAG TGAGGGAACT GAATAGCAGC 
AACACAAAGA AGTTCCTTGA GGAACGGAAA AGGCTGGCAA TGAAACAGTC GAAAGACGTT 
GATCAGCTGT TAAAAAACCA ACAAGAACAC ATGGAGAACC TGGAGAAGCA GAATGAACAG 
CATTTGAAAG CGTGCCATGC AGTGTCTCAA TCACAAGGCA AAGGAGATAC AGCAGATGGT 
GAAGCTGGAA GCAGAGATGG ACCACCGTCC AGCAACTGTT GTGTAAAGCT GCAAAATGCA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819304.1
CDS	49..3474
Translation	MAKVYEFNWQKAVPSFMQDGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRYGAVPKDPKILSALEAVGKAEQELDGKILCVCCGTDLVNISFTYMVAESAELAKQWVEGLKSITHNFRANNVSPMTCLKKHWMKLTFLTNISGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPSAFTFDKFYELTQKICPRTDTEELFKKINGDKSDYLTVDQLVSFLNEHQRDPRLNEILFPFFDAKRAMQIIETYEPDEELKKAGQISSHGFCRYLMSDENAPVFLDRLELYQEMEHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKDTAFVTSEYPVILSFENHCSKYQQYKMSKYCEEIFGDLLLSKALESHPLDTGRPLPSPNDLKRKILIKNKRLKAEVEKKQLEALKSMMEAGETATPVSILEDDIEEEIENADQEEEAHPEFKFGSELSSDDLSQKEAANIAKKMAEDAEQENNNKKGAITVEDEQAWMASYKYVGATTNIHPFLSSMINYAQPVKFQGFKVAEERNIHFNMSSFNESVGLGYLKTHALEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNDEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPADNTKNDKKGKTSNAKTNVTPQSSSEVRPSVTAAPGAGTENKKAIDIPQVKVEELKQMKAFLKQQKKQQKEIAALKKKHLKVKDIVAQHTKEFTEMVKAQNAEEQTLRDVHLSQQCELLKELLRGIHAQQMQQLKLTHDRESKEMKANQAKLSMENCKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKDVDQLLKNQQEHMENLEKQNEQHLKACHAVSQSQGKGDTADGEAGSRDGPPSSNCCVKLQNAN

Target ORF information:

RefSeq Version	XM_012963850.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C beta 4 (plcb4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963850.2

ORF Insert Sequence:

ATGGCCAAAG TGTATGAATT TAATTGGCAG AAGGCTGTTC CTTCCTTCAT GCAAGATGGG 
GCTGTGTTTG ACAGATATGA AGAAGAGTCT TTTGTGTTTG AACCCAACTG CCTCTTTAAA 
GTAGATGAAT TTGGTTTCTT TCTGACATGG AAAAGTGAAG GAAAGGAGGG TCAAGTTCTT 
GAATGTTCAC TCATCAATAG CATACGATAT GGAGCAGTAC CAAAGGACCC GAAAATCCTG 
TCTGCCCTGG AAGCCGTTGG AAAGGCTGAG CAGGAACTGG ATGGCAAGAT CCTGTGTGTT 
TGCTGCGGTA CAGATCTGGT GAACATCAGC TTTACTTACA TGGTAGCAGA GAGCGCCGAG 
CTAGCTAAGC AATGGGTAGA AGGGCTAAAA TCTATCACGC ATAATTTTAG AGCCAACAAC 
GTCAGCCCAA TGACATGTCT CAAAAAACAC TGGATGAAAT TGACATTTTT AACAAACATC 
AGTGGAAAGA TCCCAGTAAG GAGTATTACA AGAACATTTG CATCAGGAAA AACAGAAAAA 
GTCATCTTTC AAGCACTTAA AGAGTTAGGC CTTCCCAGTG GAAAGAACGA TGAAATTGAA 
CCATCGGCCT TCACCTTTGA TAAGTTTTAT GAGCTCACGC AAAAGATCTG CCCCCGCACC 
GACACAGAGG AACTATTTAA AAAAATCAAT GGAGACAAAT CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGATCCTC GTCTCAACGA GATTCTCTTC 
CCATTCTTTG ATGCCAAAAG GGCCATGCAG ATAATTGAAA CCTATGAACC AGATGAAGAG 
CTGAAGAAAG CAGGTCAGAT ATCAAGTCAT GGCTTTTGCA GGTATCTGAT GTCAGATGAA 
AATGCCCCTG TCTTCCTGGA CCGCTTAGAA CTCTACCAAG AAATGGAACA TCCCTTGGCG 
CATTACTTTA TCAGTTCCTC CCACAACACG TACCTCACTG GGAGACAGTT TGGTGGAAAA 
TCATCAGTGG AGATGTACAG ACAGGTCCTG CTGGCCGGAT GCAGGTGCGT TGAACTGGAT 
TGCTGGGATG GAAAAGGGGA AGATCAGGAG CCAATAATAA CGCATGGGAA AGCAATGTGT 
ACCGACATCC TTTTTAAGGA TGTAATCCAG GCCATCAAAG ACACCGCATT TGTCACATCA 
GAATATCCAG TGATCCTTTC CTTTGAGAAC CACTGCAGTA AATATCAGCA GTACAAGATG 
TCAAAATACT GCGAAGAAAT ATTCGGCGAC CTCTTACTGA GCAAGGCCCT CGAGAGTCAC 
CCGCTGGACA CTGGCAGACC TTTACCTTCT CCTAATGACC TCAAGCGAAA AATACTGATT 
AAAAACAAGC GGCTGAAGGC CGAAGTGGAA AAAAAACAGC TAGAAGCACT GAAAAGTATG 
ATGGAGGCTG GAGAAACAGC AACCCCAGTC AGTATCCTGG AAGATGACAT TGAAGAGGAA 
ATTGAAAACG CTGACCAAGA AGAGGAAGCA CATCCAGAAT TTAAATTCGG GAGTGAACTG 
TCTTCAGACG ACCTCAGCCA AAAGGAAGCT GCAAATATTG CCAAAAAGAT GGCTGAAGAT 
GCTGAACAAG AAAATAACAA CAAAAAGGGT GCTATAACAG TGGAGGACGA GCAAGCTTGG 
ATGGCATCCT ATAAATACGT AGGTGCCACT ACCAACATTC ATCCATTTCT GTCATCGATG 
ATCAACTACG CTCAGCCTGT AAAGTTCCAG GGCTTCAAAG TGGCTGAAGA AAGGAACATC 
CATTTCAACA TGTCTTCTTT TAACGAATCG GTTGGACTGG GATATCTAAA GACACATGCT 
CTCGAGTTTG TAAATTATAA CAAGCGTCAG ATGAGTCGGA TCTACCCTAA GGGGGGCCGA 
GTAGATTCCA GTAATTACAT GCCACAGATT TTCTGGAACG CCGGCTGCCA GATGGTTTCT 
CTGAACTATC AGACTCCAGA TTTAGCCATG CAGCTGAATC AGGGGAAGTT TGAGTACAAT 
GGATCGTGCG GGTACCTTTT GAAGCCTGAT TTCATGCGCC GTCCAGATAG AACCTTTGAC 
CCTTTCTCAG AAACACCCGT GGACGGGGTG ATTGCGGCAA CTTGTTCAGT TCAGGTCATA 
TCTGGGCAGT TCTTGTCAGA CAAGAAGATC GGGACGTACG TGGAAGTTGA TATGTATGGA 
TTACCCACGG ACACTATACG CAAGGAGTTT CGTACGCGCA TGGTCATGAA CAATGGTCTG 
AATCCCGTTT ACAATGATGA GCCCTTCGTA TTCCGAAAGG TTATCTTGCC AGACCTTGCT 
GTGCTAAGGA TAGCTGTATA TGATGACAAC AATAAACTAA TTGGGCAAAG GATACTACCT 
CTGGATGGTC TGCAGGCAGG GTACCGACAC ATTTCTCTCC GGAATGAGGG CAACAAACCA 
CTTTCCCTTC CAACAATCTT CTGTAACATT GTTTTAAAGA CCTATGTTCC GGATGGATTT 
GGAGATATTG TTGATGCATT GTCAGATCCA AAGAAATTTC TTTCTATTAC GGAGAAAAGA 
GCAGACCAGA TGAGAGCAAT GGGTATTGAA ACTAGTGACA TAGCCGATGT ACCAGCTGAC 
AACACCAAGA ATGATAAAAA AGGGAAAACC AGCAACGCCA AAACAAATGT GACCCCTCAG 
AGCAGCTCTG AAGTCAGGCC ATCCGTTACT GCTGCCCCCG GGGCAGGAAC CGAAAATAAA 
AAAGCTATCG ATATTCCTCA GGTGAAGGTA GAAGAATTAA AACAAATGAA GGCATTCCTC 
AAGCAGCAAA AGAAACAGCA GAAAGAGATT GCTGCACTAA AAAAGAAGCA CTTAAAGGTG 
AAAGATATTG TGGCTCAGCA TACAAAAGAG TTCACAGAAA TGGTGAAAGC ACAGAACGCG 
GAAGAGCAAA CACTCCGAGA TGTCCATTTA AGCCAACAGT GTGAGCTCCT GAAAGAGTTG 
CTGCGGGGGA TTCATGCACA ACAAATGCAG CAACTCAAGC TGACCCATGA CAGAGAAAGC 
AAGGAGATGA AGGCCAACCA AGCAAAATTG TCCATGGAAA ACTGCAAAGC CATAAGCCAG 
GATAAATCAA TCAAAAACAA GGCTGAGAGA GAAAGGCGAG TGAGGGAACT GAATAGCAGC 
AACACAAAGA AGTTCCTTGA GGAACGGAAA AGGCTGGCAA TGAAACAGTC GAAAGACGTT 
GATCAGCTGT TAAAAAACCA ACAAGAACAC ATGGAGAACC TGGAGAAGCA GAATGAACAG 
CATTTGAAAG CGTGCCATGC AGTGTCTCAA TCACAAGGCA AAGGAGATAC AGCAGATGGT 
GAAGCTGGAA GCAGAGATGG ACCACCGTCC AGCAACTGTT GTGTAAAGCT GCAAAATGCA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	plcb4
Entrez Gene ID	100125098
Full Name	phospholipase C beta 4
General protein information	Preferred Names phospholipase C beta 4 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	PLCB4	XP_002798260.1
Canis lupus familiaris (dog)	PLCB4	XP_859719.2
Bos taurus (cattle)	PLCB4	NP_001159982.1
Mus musculus (house mouse)	Plcb4	NP_038857.1
Rattus norvegicus (Norway rat)	Plcb4	NP_077329.1
Gallus gallus (chicken)	PLCB4	NP_001186364.1
Danio rerio (zebrafish)	LOC100537538	XP_003200348.2
Homo sapiens (human)	PLCB4	NP_877949.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001936	XP_003435972.1
Drosophila melanogaster (fruit fly)	norpA	NP_001014720.1
Xenopus tropicalis (tropical clawed frog)	plcb4	XP_004914780.1
Pan troglodytes (chimpanzee)	PLCB4	XP_001168348.2
Caenorhabditis elegans (roundworm)	egl-8	NP_001021346.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

plcb4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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