pdgfra cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pdgfra
Entrez Gene ID	100124862
Full Name	platelet-derived growth factor receptor, alpha polypeptide
Synonyms	pdgfr-alpha,pdgfr2,pdgfralpha
General protein information	Preferred Names platelet-derived growth factor receptor, alpha polypeptide Names platelet-derived growth factor receptor alpha
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001102820.1	NP_001096290.1	platelet-derived growth factor receptor alpha precursor
XM_012963365.3	XP_012818819.2	platelet-derived growth factor receptor alpha isoform X1
XM_031900856.1	XP_031756716.1	platelet-derived growth factor receptor alpha isoform X1
XM_012963365.2	XP_012818819.1	platelet-derived growth factor receptor alpha isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pdgfra	NP_036934.1
Caenorhabditis elegans (roundworm)	C24G6.2	NP_504459.1
Caenorhabditis elegans (roundworm)	kin-9	NP_001257058.1
Xenopus tropicalis (tropical clawed frog)	LOC100124862	NP_001096290.1
	PDGFRA	XP_002808459.1
Canis lupus familiaris (dog)	PDGFRA	XP_005628252.1
Mus musculus (house mouse)	Pdgfra	NP_001076785.1
Pan troglodytes (chimpanzee)	PDGFRA	XP_003950404.1
Bos taurus (cattle)	PDGFRA	NP_001179274.1
Homo sapiens (human)	PDGFRA	NP_006197.1
Gallus gallus (chicken)	PDGFRA	NP_990080.1
Danio rerio (zebrafish)	pdgfra	NP_571534.1
Caenorhabditis elegans (roundworm)	F09A5.2	NP_510182.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pdgfra gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pdgfra cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa47423	XM_031900856.1 Latest version!	Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa47423	XM_012963365.3 Latest version!	Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa21105	NM_001102820.1 Latest version!	Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa30487	XM_012963365.2 Latest version!	Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pdgfra ( XM_012963365.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818819.2 Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa47423
Clone ID Related Accession (Same CDS sequence)	XM_012963365.3 , XM_031900856.1
Accession Version	XM_012963365.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	platelet-derived growth factor receptor alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963365.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCTA CCACGAGGGC ATCCCTTGTT TTGGGGTGTC TCCTGATTAT AGGTCCGTGG 
GTCATTCTTG CTCAAAATCC CCTGCCCTCA ATATTTCCTG AAAAGGATGA GTTGGTTCAG 
AGGTTGCACT CCTCCTTTTC ACTAACGTGC ACTGGTGAGA GTGAACTGAG CTGGCAGCAC 
CCCCATTCCG AATCTGAACT GGGGAATGTA GTCATTGGAA GCGAAGAAAA CAACAGCGGC 
CTCTTTGTGT CTGTTCTGGA AGTGACCAAC GCTTCCGCCT CCAATACGGG CCTCTACACC 
TGCTACTACA ATCACACCCA GACTGAGGAC AGTGAGATTG AAGGCACTGA TATCTACATC 
TATGTCCCAG ACACCAATGT CCCTTTTGTT CCCCCCGGGT TGTTTGACCA TATCATTGTG 
GTTGAGGAAG ATGAATCTGC TTTCATACCT TGTCGCACTA CCGACCCTTC CAGCCAAGTG 
ACCCTAAAAA GTATCGAAAA TTCTAAGACT GTCAGTGCCT TTTATGACAG CAAGCAAGGA 
TTTGTTGGAA ACTTCCAACC AGGATCATAT ATCTGTGAAA CCGTCTCTAA CAAAATGGTC 
TACCAGACAG ACCTTTACAT TCTACAGACA TGGAAAGCAA CGCACAACAT TTCCGTTGAG 
ATGGAAGCCC CCCAAACTGT ATTTCGAACT GGGGAAACCA TTACCATTTC CTGCATTGTG 
CTGGAGAATG AGGTGGTAGA ATTGAAGTGG TACTACCCTG GACAACAGAG AGGCGTTGGC 
AAAAGAAATG TTGAAGAAAG CAGGGTTCCA TCTCAGAGAC TGGTGTACAC CTTAACCATT 
GCAAATGCCA CTGCGGATGA CTCAGGAGAG TACCAGTGTG CCGTTCTACA TGCAACACTA 
GATAACAAGG TCATCAAGAA GACAAATATA ACAGTTCATG AAAAGGGATT CATCAATCTG 
GAGCCCATGT TTGGATCTGA GGAATATGCC AACCTCCATG AAGTGAAGAG CTTTATAGTA 
GACTTGCATG CCTATCCCTG CCCTAGGGTC TTCTGGTTGA AGGACAACCG GACACTGAGT 
GAAAATCTGA CAGAGATCAC GACTAGCACT GTTACAACTA AAGAAACCAG GTTTCAGAGT 
AAACTTAAAC TGATCCGGGC CAAAGAAGAA GACAGCGGAC TGTACACTCT TGTGGCACAA 
AATGACGCAG AAACTAAAAG CTACTCGTTT ATTTTGCAGA TAAAAGTACC CGCTTTGATA 
TTGGAACTGG TTGATGAACA TCATGGGACA TCTGGTGAAC AGACTGTTGG GTGCTTAGCC 
AAAGGGATGC CGGTCCCTGA TGTGGAATGG TTGGTCTGCA AAGACATCAA AAGATGCAAC 
AATGACACCT TGTGGTCCCT TCTGGCAATA AATGGGTCAG AAATCAGTAT GGAGACCCAT 
CAAGATTATG AGAACACAAT AGAAAGCCAG GTGACCTTTA AGAAAGTTGA GGAAACAATG 
GCAATCCGAT GTATCGCAAG AAACGACCTC GGTGTGGTGG CCAGGGAACT AAAGCTGGTT 
GCACCCACCC TACGTTCTGA ACTAACTGTG GCAGCCGCCG TCCTTGTCCT TCTGGTGATT 
GTCATCATAT CTCTCATTGT ACTGGTCATC ATATGGAAAC AGAAACCAAG GTATGAAATA 
AGATGGCGAG TGATCGAGTC CATCAGCCCA GATGGCCATG AATATATCTA TGTGGACCCA 
ATGCAGCTTC CTTATGACTC TCGGTGGGAG TTCCAAAGGG ATGGACTTGT TCTTGGTCGC 
ATCCTTGGCT CTGGGGCTTT TGGGAAAGTG GTTGAAGGCA CAGCATATGG GTTAAGTCGT 
TCTCAGCCAG TTATGAAAGT TGCTGTAAAA ATGTTGAAAC CTACTGCCCG GTCAAGCGAA 
AAGCAGGCAC TGATGTCTGA ACTGAAGATC ATGACACACC TTGGCGCACA TCTCAATATA 
GTCAATTTAT TAGGAGCCTG TACTAAGTCA GGACCAATTT ATATCATAAC TGAATACTGC 
TTCTATGGTG ACCTCGTGAA TTACCTGCAT AAGAACCGAG ATAATTTTCA GAGCCGACAC 
CCAGAGAAAT CAAAGAAGGA CTTGGACATC TTTGGGCTGA ACCCAGCTGA CGAGAGTACG 
AGAAGCTATG TTATCTTATC ATTTGAAAAC AACGGCGACT ATATGGACAT GAAACAAGCT 
GACACCATGC AGTATGTACC CATGCTGGAA ATGAAAGAAC CCTCCAAGTA TTCAGATATC 
CAGAGATCCT TGTATGACCG GCCTGCGTCG TACAAGAAGA AGCCTTTGTC AGAAGTGAAA 
AATATCTTGT CTGATGATGG ATTCGATGGA TTCGATGGTC TAACAGTACT GGACTTGCTC 
AGTTTCACAT ATCAAGTTGC AAGGGGCATG GAATTCTTAG CCTCTAAAAA TTGTGTACAT 
CGGGATTTGG CAGCTCGGAA TGTCCTATTA GCACATGGGA AAATTGTAAA AATCTGTGAC 
TTTGGCCTAG CAAGAGACAT CATGCACGAC TCCAACTATG TATCCAAAGG CAGCACTTTC 
CTTCCAGTAA AGTGGATGGC GCCCGAAAGC ATCTTCGATA ACCTGTACAC AACTCTCAGC 
GATGTGTGGT CATTTGGAAT CCTGCTGTGG GAAATATTTT CTCTTGGGGG CACACCGTAT 
CCTGGCATGA TTGTGGATTC AACCTTCTAT AATAAGATAA AAAGTGGCTA CAGAATGGCA 
AAACCTGACC ATGCCACCCA TGAAGTGTAC GACATCATGG TAAAATGTTG GAACAGTGAA 
CCAGAAAAAA GGCCATCATT TCGCCATCTG AGTGACATTG TGGAAAGTCT ACTTCCTATG 
GAATACAAAA GGTGCTACGA GACAGTTCTT CACGATTTCC TTAAAAGTGA CCACCCTGCA 
GTTACTCGCA TGAGAAGTGA CAGTGACAAT TCCTACATTG GGGTGACGTA TAAGAATGAA 
AACAAAATGA AGGACAGAGA GAGTGGGTTT GATGAGCAAA GGCTGAGCGC TGACAGCGGT 
TACATTATTC CCCTTCCTGA TATTGATCCC GTTTCAGAAG ATGAGTCCAG CAAGAGGAAC 
AGGCACAGTT CTCAAACATC AGAAGAAAGT GCAATCGAGA CCGGGTCCAG CAGTTCCACT 
TTTATAAAGA GGGAGGACGA GACCATTGAA GACATTGAGA TGATGGACGA CATTGGGATC 
GATTCCTCGG ATCTAGTGGA GGACAGTTTC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818819.2
CDS	876..4151
Translation	MMPTTRASLVLGCLLIIGPWVILAQNPLPSIFPEKDELVQRLHSSFSLTCTGESELSWQHPHSESELGNVVIGSEENNSGLFVSVLEVTNASASNTGLYTCYYNHTQTEDSEIEGTDIYIYVPDTNVPFVPPGLFDHIIVVEEDESAFIPCRTTDPSSQVTLKSIENSKTVSAFYDSKQGFVGNFQPGSYICETVSNKMVYQTDLYILQTWKATHNISVEMEAPQTVFRTGETITISCIVLENEVVELKWYYPGQQRGVGKRNVEESRVPSQRLVYTLTIANATADDSGEYQCAVLHATLDNKVIKKTNITVHEKGFINLEPMFGSEEYANLHEVKSFIVDLHAYPCPRVFWLKDNRTLSENLTEITTSTVTTKETRFQSKLKLIRAKEEDSGLYTLVAQNDAETKSYSFILQIKVPALILELVDEHHGTSGEQTVGCLAKGMPVPDVEWLVCKDIKRCNNDTLWSLLAINGSEISMETHQDYENTIESQVTFKKVEETMAIRCIARNDLGVVARELKLVAPTLRSELTVAAAVLVLLVIVIISLIVLVIIWKQKPRYEIRWRVIESISPDGHEYIYVDPMQLPYDSRWEFQRDGLVLGRILGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLGAHLNIVNLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDNFQSRHPEKSKKDLDIFGLNPADESTRSYVILSFENNGDYMDMKQADTMQYVPMLEMKEPSKYSDIQRSLYDRPASYKKKPLSEVKNILSDDGFDGFDGLTVLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAHGKIVKICDFGLARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSFGILLWEIFSLGGTPYPGMIVDSTFYNKIKSGYRMAKPDHATHEVYDIMVKCWNSEPEKRPSFRHLSDIVESLLPMEYKRCYETVLHDFLKSDHPAVTRMRSDSDNSYIGVTYKNENKMKDRESGFDEQRLSADSGYIIPLPDIDPVSEDESSKRNRHSSQTSEESAIETGSSSSTFIKREDETIEDIEMMDDIGIDSSDLVEDSFL

Target ORF information:

RefSeq Version	XM_012963365.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963365.3

ORF Insert Sequence:

ATGATGCCTA CCACGAGGGC ATCCCTTGTT TTGGGGTGTC TCCTGATTAT AGGTCCGTGG 
GTCATTCTTG CTCAAAATCC CCTGCCCTCA ATATTTCCTG AAAAGGATGA GTTGGTTCAG 
AGGTTGCACT CCTCCTTTTC ACTAACGTGC ACTGGTGAGA GTGAACTGAG CTGGCAGCAC 
CCCCATTCCG AATCTGAACT GGGGAATGTA GTCATTGGAA GCGAAGAAAA CAACAGCGGC 
CTCTTTGTGT CTGTTCTGGA AGTGACCAAC GCTTCCGCCT CCAATACGGG CCTCTACACC 
TGCTACTACA ATCACACCCA GACTGAGGAC AGTGAGATTG AAGGCACTGA TATCTACATC 
TATGTCCCAG ACACCAATGT CCCTTTTGTT CCCCCCGGGT TGTTTGACCA TATCATTGTG 
GTTGAGGAAG ATGAATCTGC TTTCATACCT TGTCGCACTA CCGACCCTTC CAGCCAAGTG 
ACCCTAAAAA GTATCGAAAA TTCTAAGACT GTCAGTGCCT TTTATGACAG CAAGCAAGGA 
TTTGTTGGAA ACTTCCAACC AGGATCATAT ATCTGTGAAA CCGTCTCTAA CAAAATGGTC 
TACCAGACAG ACCTTTACAT TCTACAGACA TGGAAAGCAA CGCACAACAT TTCCGTTGAG 
ATGGAAGCCC CCCAAACTGT ATTTCGAACT GGGGAAACCA TTACCATTTC CTGCATTGTG 
CTGGAGAATG AGGTGGTAGA ATTGAAGTGG TACTACCCTG GACAACAGAG AGGCGTTGGC 
AAAAGAAATG TTGAAGAAAG CAGGGTTCCA TCTCAGAGAC TGGTGTACAC CTTAACCATT 
GCAAATGCCA CTGCGGATGA CTCAGGAGAG TACCAGTGTG CCGTTCTACA TGCAACACTA 
GATAACAAGG TCATCAAGAA GACAAATATA ACAGTTCATG AAAAGGGATT CATCAATCTG 
GAGCCCATGT TTGGATCTGA GGAATATGCC AACCTCCATG AAGTGAAGAG CTTTATAGTA 
GACTTGCATG CCTATCCCTG CCCTAGGGTC TTCTGGTTGA AGGACAACCG GACACTGAGT 
GAAAATCTGA CAGAGATCAC GACTAGCACT GTTACAACTA AAGAAACCAG GTTTCAGAGT 
AAACTTAAAC TGATCCGGGC CAAAGAAGAA GACAGCGGAC TGTACACTCT TGTGGCACAA 
AATGACGCAG AAACTAAAAG CTACTCGTTT ATTTTGCAGA TAAAAGTACC CGCTTTGATA 
TTGGAACTGG TTGATGAACA TCATGGGACA TCTGGTGAAC AGACTGTTGG GTGCTTAGCC 
AAAGGGATGC CGGTCCCTGA TGTGGAATGG TTGGTCTGCA AAGACATCAA AAGATGCAAC 
AATGACACCT TGTGGTCCCT TCTGGCAATA AATGGGTCAG AAATCAGTAT GGAGACCCAT 
CAAGATTATG AGAACACAAT AGAAAGCCAG GTGACCTTTA AGAAAGTTGA GGAAACAATG 
GCAATCCGAT GTATCGCAAG AAACGACCTC GGTGTGGTGG CCAGGGAACT AAAGCTGGTT 
GCACCCACCC TACGTTCTGA ACTAACTGTG GCAGCCGCCG TCCTTGTCCT TCTGGTGATT 
GTCATCATAT CTCTCATTGT ACTGGTCATC ATATGGAAAC AGAAACCAAG GTATGAAATA 
AGATGGCGAG TGATCGAGTC CATCAGCCCA GATGGCCATG AATATATCTA TGTGGACCCA 
ATGCAGCTTC CTTATGACTC TCGGTGGGAG TTCCAAAGGG ATGGACTTGT TCTTGGTCGC 
ATCCTTGGCT CTGGGGCTTT TGGGAAAGTG GTTGAAGGCA CAGCATATGG GTTAAGTCGT 
TCTCAGCCAG TTATGAAAGT TGCTGTAAAA ATGTTGAAAC CTACTGCCCG GTCAAGCGAA 
AAGCAGGCAC TGATGTCTGA ACTGAAGATC ATGACACACC TTGGCGCACA TCTCAATATA 
GTCAATTTAT TAGGAGCCTG TACTAAGTCA GGACCAATTT ATATCATAAC TGAATACTGC 
TTCTATGGTG ACCTCGTGAA TTACCTGCAT AAGAACCGAG ATAATTTTCA GAGCCGACAC 
CCAGAGAAAT CAAAGAAGGA CTTGGACATC TTTGGGCTGA ACCCAGCTGA CGAGAGTACG 
AGAAGCTATG TTATCTTATC ATTTGAAAAC AACGGCGACT ATATGGACAT GAAACAAGCT 
GACACCATGC AGTATGTACC CATGCTGGAA ATGAAAGAAC CCTCCAAGTA TTCAGATATC 
CAGAGATCCT TGTATGACCG GCCTGCGTCG TACAAGAAGA AGCCTTTGTC AGAAGTGAAA 
AATATCTTGT CTGATGATGG ATTCGATGGA TTCGATGGTC TAACAGTACT GGACTTGCTC 
AGTTTCACAT ATCAAGTTGC AAGGGGCATG GAATTCTTAG CCTCTAAAAA TTGTGTACAT 
CGGGATTTGG CAGCTCGGAA TGTCCTATTA GCACATGGGA AAATTGTAAA AATCTGTGAC 
TTTGGCCTAG CAAGAGACAT CATGCACGAC TCCAACTATG TATCCAAAGG CAGCACTTTC 
CTTCCAGTAA AGTGGATGGC GCCCGAAAGC ATCTTCGATA ACCTGTACAC AACTCTCAGC 
GATGTGTGGT CATTTGGAAT CCTGCTGTGG GAAATATTTT CTCTTGGGGG CACACCGTAT 
CCTGGCATGA TTGTGGATTC AACCTTCTAT AATAAGATAA AAAGTGGCTA CAGAATGGCA 
AAACCTGACC ATGCCACCCA TGAAGTGTAC GACATCATGG TAAAATGTTG GAACAGTGAA 
CCAGAAAAAA GGCCATCATT TCGCCATCTG AGTGACATTG TGGAAAGTCT ACTTCCTATG 
GAATACAAAA GGTGCTACGA GACAGTTCTT CACGATTTCC TTAAAAGTGA CCACCCTGCA 
GTTACTCGCA TGAGAAGTGA CAGTGACAAT TCCTACATTG GGGTGACGTA TAAGAATGAA 
AACAAAATGA AGGACAGAGA GAGTGGGTTT GATGAGCAAA GGCTGAGCGC TGACAGCGGT 
TACATTATTC CCCTTCCTGA TATTGATCCC GTTTCAGAAG ATGAGTCCAG CAAGAGGAAC 
AGGCACAGTT CTCAAACATC AGAAGAAAGT GCAATCGAGA CCGGGTCCAG CAGTTCCACT 
TTTATAAAGA GGGAGGACGA GACCATTGAA GACATTGAGA TGATGGACGA CATTGGGATC 
GATTCCTCGG ATCTAGTGGA GGACAGTTTC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pdgfra ( XM_031900856.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756716.1 Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa47423
Clone ID Related Accession (Same CDS sequence)	XM_012963365.3 , XM_031900856.1
Accession Version	XM_031900856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	platelet-derived growth factor receptor alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCTA CCACGAGGGC ATCCCTTGTT TTGGGGTGTC TCCTGATTAT AGGTCCGTGG 
GTCATTCTTG CTCAAAATCC CCTGCCCTCA ATATTTCCTG AAAAGGATGA GTTGGTTCAG 
AGGTTGCACT CCTCCTTTTC ACTAACGTGC ACTGGTGAGA GTGAACTGAG CTGGCAGCAC 
CCCCATTCCG AATCTGAACT GGGGAATGTA GTCATTGGAA GCGAAGAAAA CAACAGCGGC 
CTCTTTGTGT CTGTTCTGGA AGTGACCAAC GCTTCCGCCT CCAATACGGG CCTCTACACC 
TGCTACTACA ATCACACCCA GACTGAGGAC AGTGAGATTG AAGGCACTGA TATCTACATC 
TATGTCCCAG ACACCAATGT CCCTTTTGTT CCCCCCGGGT TGTTTGACCA TATCATTGTG 
GTTGAGGAAG ATGAATCTGC TTTCATACCT TGTCGCACTA CCGACCCTTC CAGCCAAGTG 
ACCCTAAAAA GTATCGAAAA TTCTAAGACT GTCAGTGCCT TTTATGACAG CAAGCAAGGA 
TTTGTTGGAA ACTTCCAACC AGGATCATAT ATCTGTGAAA CCGTCTCTAA CAAAATGGTC 
TACCAGACAG ACCTTTACAT TCTACAGACA TGGAAAGCAA CGCACAACAT TTCCGTTGAG 
ATGGAAGCCC CCCAAACTGT ATTTCGAACT GGGGAAACCA TTACCATTTC CTGCATTGTG 
CTGGAGAATG AGGTGGTAGA ATTGAAGTGG TACTACCCTG GACAACAGAG AGGCGTTGGC 
AAAAGAAATG TTGAAGAAAG CAGGGTTCCA TCTCAGAGAC TGGTGTACAC CTTAACCATT 
GCAAATGCCA CTGCGGATGA CTCAGGAGAG TACCAGTGTG CCGTTCTACA TGCAACACTA 
GATAACAAGG TCATCAAGAA GACAAATATA ACAGTTCATG AAAAGGGATT CATCAATCTG 
GAGCCCATGT TTGGATCTGA GGAATATGCC AACCTCCATG AAGTGAAGAG CTTTATAGTA 
GACTTGCATG CCTATCCCTG CCCTAGGGTC TTCTGGTTGA AGGACAACCG GACACTGAGT 
GAAAATCTGA CAGAGATCAC GACTAGCACT GTTACAACTA AAGAAACCAG GTTTCAGAGT 
AAACTTAAAC TGATCCGGGC CAAAGAAGAA GACAGCGGAC TGTACACTCT TGTGGCACAA 
AATGACGCAG AAACTAAAAG CTACTCGTTT ATTTTGCAGA TAAAAGTACC CGCTTTGATA 
TTGGAACTGG TTGATGAACA TCATGGGACA TCTGGTGAAC AGACTGTTGG GTGCTTAGCC 
AAAGGGATGC CGGTCCCTGA TGTGGAATGG TTGGTCTGCA AAGACATCAA AAGATGCAAC 
AATGACACCT TGTGGTCCCT TCTGGCAATA AATGGGTCAG AAATCAGTAT GGAGACCCAT 
CAAGATTATG AGAACACAAT AGAAAGCCAG GTGACCTTTA AGAAAGTTGA GGAAACAATG 
GCAATCCGAT GTATCGCAAG AAACGACCTC GGTGTGGTGG CCAGGGAACT AAAGCTGGTT 
GCACCCACCC TACGTTCTGA ACTAACTGTG GCAGCCGCCG TCCTTGTCCT TCTGGTGATT 
GTCATCATAT CTCTCATTGT ACTGGTCATC ATATGGAAAC AGAAACCAAG GTATGAAATA 
AGATGGCGAG TGATCGAGTC CATCAGCCCA GATGGCCATG AATATATCTA TGTGGACCCA 
ATGCAGCTTC CTTATGACTC TCGGTGGGAG TTCCAAAGGG ATGGACTTGT TCTTGGTCGC 
ATCCTTGGCT CTGGGGCTTT TGGGAAAGTG GTTGAAGGCA CAGCATATGG GTTAAGTCGT 
TCTCAGCCAG TTATGAAAGT TGCTGTAAAA ATGTTGAAAC CTACTGCCCG GTCAAGCGAA 
AAGCAGGCAC TGATGTCTGA ACTGAAGATC ATGACACACC TTGGCGCACA TCTCAATATA 
GTCAATTTAT TAGGAGCCTG TACTAAGTCA GGACCAATTT ATATCATAAC TGAATACTGC 
TTCTATGGTG ACCTCGTGAA TTACCTGCAT AAGAACCGAG ATAATTTTCA GAGCCGACAC 
CCAGAGAAAT CAAAGAAGGA CTTGGACATC TTTGGGCTGA ACCCAGCTGA CGAGAGTACG 
AGAAGCTATG TTATCTTATC ATTTGAAAAC AACGGCGACT ATATGGACAT GAAACAAGCT 
GACACCATGC AGTATGTACC CATGCTGGAA ATGAAAGAAC CCTCCAAGTA TTCAGATATC 
CAGAGATCCT TGTATGACCG GCCTGCGTCG TACAAGAAGA AGCCTTTGTC AGAAGTGAAA 
AATATCTTGT CTGATGATGG ATTCGATGGA TTCGATGGTC TAACAGTACT GGACTTGCTC 
AGTTTCACAT ATCAAGTTGC AAGGGGCATG GAATTCTTAG CCTCTAAAAA TTGTGTACAT 
CGGGATTTGG CAGCTCGGAA TGTCCTATTA GCACATGGGA AAATTGTAAA AATCTGTGAC 
TTTGGCCTAG CAAGAGACAT CATGCACGAC TCCAACTATG TATCCAAAGG CAGCACTTTC 
CTTCCAGTAA AGTGGATGGC GCCCGAAAGC ATCTTCGATA ACCTGTACAC AACTCTCAGC 
GATGTGTGGT CATTTGGAAT CCTGCTGTGG GAAATATTTT CTCTTGGGGG CACACCGTAT 
CCTGGCATGA TTGTGGATTC AACCTTCTAT AATAAGATAA AAAGTGGCTA CAGAATGGCA 
AAACCTGACC ATGCCACCCA TGAAGTGTAC GACATCATGG TAAAATGTTG GAACAGTGAA 
CCAGAAAAAA GGCCATCATT TCGCCATCTG AGTGACATTG TGGAAAGTCT ACTTCCTATG 
GAATACAAAA GGTGCTACGA GACAGTTCTT CACGATTTCC TTAAAAGTGA CCACCCTGCA 
GTTACTCGCA TGAGAAGTGA CAGTGACAAT TCCTACATTG GGGTGACGTA TAAGAATGAA 
AACAAAATGA AGGACAGAGA GAGTGGGTTT GATGAGCAAA GGCTGAGCGC TGACAGCGGT 
TACATTATTC CCCTTCCTGA TATTGATCCC GTTTCAGAAG ATGAGTCCAG CAAGAGGAAC 
AGGCACAGTT CTCAAACATC AGAAGAAAGT GCAATCGAGA CCGGGTCCAG CAGTTCCACT 
TTTATAAAGA GGGAGGACGA GACCATTGAA GACATTGAGA TGATGGACGA CATTGGGATC 
GATTCCTCGG ATCTAGTGGA GGACAGTTTC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756716.1
CDS	77..3352
Translation	MMPTTRASLVLGCLLIIGPWVILAQNPLPSIFPEKDELVQRLHSSFSLTCTGESELSWQHPHSESELGNVVIGSEENNSGLFVSVLEVTNASASNTGLYTCYYNHTQTEDSEIEGTDIYIYVPDTNVPFVPPGLFDHIIVVEEDESAFIPCRTTDPSSQVTLKSIENSKTVSAFYDSKQGFVGNFQPGSYICETVSNKMVYQTDLYILQTWKATHNISVEMEAPQTVFRTGETITISCIVLENEVVELKWYYPGQQRGVGKRNVEESRVPSQRLVYTLTIANATADDSGEYQCAVLHATLDNKVIKKTNITVHEKGFINLEPMFGSEEYANLHEVKSFIVDLHAYPCPRVFWLKDNRTLSENLTEITTSTVTTKETRFQSKLKLIRAKEEDSGLYTLVAQNDAETKSYSFILQIKVPALILELVDEHHGTSGEQTVGCLAKGMPVPDVEWLVCKDIKRCNNDTLWSLLAINGSEISMETHQDYENTIESQVTFKKVEETMAIRCIARNDLGVVARELKLVAPTLRSELTVAAAVLVLLVIVIISLIVLVIIWKQKPRYEIRWRVIESISPDGHEYIYVDPMQLPYDSRWEFQRDGLVLGRILGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLGAHLNIVNLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDNFQSRHPEKSKKDLDIFGLNPADESTRSYVILSFENNGDYMDMKQADTMQYVPMLEMKEPSKYSDIQRSLYDRPASYKKKPLSEVKNILSDDGFDGFDGLTVLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAHGKIVKICDFGLARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSFGILLWEIFSLGGTPYPGMIVDSTFYNKIKSGYRMAKPDHATHEVYDIMVKCWNSEPEKRPSFRHLSDIVESLLPMEYKRCYETVLHDFLKSDHPAVTRMRSDSDNSYIGVTYKNENKMKDRESGFDEQRLSADSGYIIPLPDIDPVSEDESSKRNRHSSQTSEESAIETGSSSSTFIKREDETIEDIEMMDDIGIDSSDLVEDSFL

Target ORF information:

RefSeq Version	XM_031900856.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900856.1

ORF Insert Sequence:

ATGATGCCTA CCACGAGGGC ATCCCTTGTT TTGGGGTGTC TCCTGATTAT AGGTCCGTGG 
GTCATTCTTG CTCAAAATCC CCTGCCCTCA ATATTTCCTG AAAAGGATGA GTTGGTTCAG 
AGGTTGCACT CCTCCTTTTC ACTAACGTGC ACTGGTGAGA GTGAACTGAG CTGGCAGCAC 
CCCCATTCCG AATCTGAACT GGGGAATGTA GTCATTGGAA GCGAAGAAAA CAACAGCGGC 
CTCTTTGTGT CTGTTCTGGA AGTGACCAAC GCTTCCGCCT CCAATACGGG CCTCTACACC 
TGCTACTACA ATCACACCCA GACTGAGGAC AGTGAGATTG AAGGCACTGA TATCTACATC 
TATGTCCCAG ACACCAATGT CCCTTTTGTT CCCCCCGGGT TGTTTGACCA TATCATTGTG 
GTTGAGGAAG ATGAATCTGC TTTCATACCT TGTCGCACTA CCGACCCTTC CAGCCAAGTG 
ACCCTAAAAA GTATCGAAAA TTCTAAGACT GTCAGTGCCT TTTATGACAG CAAGCAAGGA 
TTTGTTGGAA ACTTCCAACC AGGATCATAT ATCTGTGAAA CCGTCTCTAA CAAAATGGTC 
TACCAGACAG ACCTTTACAT TCTACAGACA TGGAAAGCAA CGCACAACAT TTCCGTTGAG 
ATGGAAGCCC CCCAAACTGT ATTTCGAACT GGGGAAACCA TTACCATTTC CTGCATTGTG 
CTGGAGAATG AGGTGGTAGA ATTGAAGTGG TACTACCCTG GACAACAGAG AGGCGTTGGC 
AAAAGAAATG TTGAAGAAAG CAGGGTTCCA TCTCAGAGAC TGGTGTACAC CTTAACCATT 
GCAAATGCCA CTGCGGATGA CTCAGGAGAG TACCAGTGTG CCGTTCTACA TGCAACACTA 
GATAACAAGG TCATCAAGAA GACAAATATA ACAGTTCATG AAAAGGGATT CATCAATCTG 
GAGCCCATGT TTGGATCTGA GGAATATGCC AACCTCCATG AAGTGAAGAG CTTTATAGTA 
GACTTGCATG CCTATCCCTG CCCTAGGGTC TTCTGGTTGA AGGACAACCG GACACTGAGT 
GAAAATCTGA CAGAGATCAC GACTAGCACT GTTACAACTA AAGAAACCAG GTTTCAGAGT 
AAACTTAAAC TGATCCGGGC CAAAGAAGAA GACAGCGGAC TGTACACTCT TGTGGCACAA 
AATGACGCAG AAACTAAAAG CTACTCGTTT ATTTTGCAGA TAAAAGTACC CGCTTTGATA 
TTGGAACTGG TTGATGAACA TCATGGGACA TCTGGTGAAC AGACTGTTGG GTGCTTAGCC 
AAAGGGATGC CGGTCCCTGA TGTGGAATGG TTGGTCTGCA AAGACATCAA AAGATGCAAC 
AATGACACCT TGTGGTCCCT TCTGGCAATA AATGGGTCAG AAATCAGTAT GGAGACCCAT 
CAAGATTATG AGAACACAAT AGAAAGCCAG GTGACCTTTA AGAAAGTTGA GGAAACAATG 
GCAATCCGAT GTATCGCAAG AAACGACCTC GGTGTGGTGG CCAGGGAACT AAAGCTGGTT 
GCACCCACCC TACGTTCTGA ACTAACTGTG GCAGCCGCCG TCCTTGTCCT TCTGGTGATT 
GTCATCATAT CTCTCATTGT ACTGGTCATC ATATGGAAAC AGAAACCAAG GTATGAAATA 
AGATGGCGAG TGATCGAGTC CATCAGCCCA GATGGCCATG AATATATCTA TGTGGACCCA 
ATGCAGCTTC CTTATGACTC TCGGTGGGAG TTCCAAAGGG ATGGACTTGT TCTTGGTCGC 
ATCCTTGGCT CTGGGGCTTT TGGGAAAGTG GTTGAAGGCA CAGCATATGG GTTAAGTCGT 
TCTCAGCCAG TTATGAAAGT TGCTGTAAAA ATGTTGAAAC CTACTGCCCG GTCAAGCGAA 
AAGCAGGCAC TGATGTCTGA ACTGAAGATC ATGACACACC TTGGCGCACA TCTCAATATA 
GTCAATTTAT TAGGAGCCTG TACTAAGTCA GGACCAATTT ATATCATAAC TGAATACTGC 
TTCTATGGTG ACCTCGTGAA TTACCTGCAT AAGAACCGAG ATAATTTTCA GAGCCGACAC 
CCAGAGAAAT CAAAGAAGGA CTTGGACATC TTTGGGCTGA ACCCAGCTGA CGAGAGTACG 
AGAAGCTATG TTATCTTATC ATTTGAAAAC AACGGCGACT ATATGGACAT GAAACAAGCT 
GACACCATGC AGTATGTACC CATGCTGGAA ATGAAAGAAC CCTCCAAGTA TTCAGATATC 
CAGAGATCCT TGTATGACCG GCCTGCGTCG TACAAGAAGA AGCCTTTGTC AGAAGTGAAA 
AATATCTTGT CTGATGATGG ATTCGATGGA TTCGATGGTC TAACAGTACT GGACTTGCTC 
AGTTTCACAT ATCAAGTTGC AAGGGGCATG GAATTCTTAG CCTCTAAAAA TTGTGTACAT 
CGGGATTTGG CAGCTCGGAA TGTCCTATTA GCACATGGGA AAATTGTAAA AATCTGTGAC 
TTTGGCCTAG CAAGAGACAT CATGCACGAC TCCAACTATG TATCCAAAGG CAGCACTTTC 
CTTCCAGTAA AGTGGATGGC GCCCGAAAGC ATCTTCGATA ACCTGTACAC AACTCTCAGC 
GATGTGTGGT CATTTGGAAT CCTGCTGTGG GAAATATTTT CTCTTGGGGG CACACCGTAT 
CCTGGCATGA TTGTGGATTC AACCTTCTAT AATAAGATAA AAAGTGGCTA CAGAATGGCA 
AAACCTGACC ATGCCACCCA TGAAGTGTAC GACATCATGG TAAAATGTTG GAACAGTGAA 
CCAGAAAAAA GGCCATCATT TCGCCATCTG AGTGACATTG TGGAAAGTCT ACTTCCTATG 
GAATACAAAA GGTGCTACGA GACAGTTCTT CACGATTTCC TTAAAAGTGA CCACCCTGCA 
GTTACTCGCA TGAGAAGTGA CAGTGACAAT TCCTACATTG GGGTGACGTA TAAGAATGAA 
AACAAAATGA AGGACAGAGA GAGTGGGTTT GATGAGCAAA GGCTGAGCGC TGACAGCGGT 
TACATTATTC CCCTTCCTGA TATTGATCCC GTTTCAGAAG ATGAGTCCAG CAAGAGGAAC 
AGGCACAGTT CTCAAACATC AGAAGAAAGT GCAATCGAGA CCGGGTCCAG CAGTTCCACT 
TTTATAAAGA GGGAGGACGA GACCATTGAA GACATTGAGA TGATGGACGA CATTGGGATC 
GATTCCTCGG ATCTAGTGGA GGACAGTTTC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pdgfra ( NM_001102820.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001096290.1 Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), mRNA.

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CloneID	OXa21105
Clone ID Related Accession (Same CDS sequence)	NM_001102820.1
Accession Version	NM_001102820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	platelet-derived growth factor receptor alpha precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC135576.1. ##Evidence-Data-START## Transcript exon combination :: BC135576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGATGCCTA CCACGAGGGC ATCCCTTGTT TTGGGGTGTC TCCTGATTAT AGGTCCGTGG 
GTCATTCTTG CTCAAAATCC CCTGCCCTCA ATATTTCCTG AAAAGGATGA GTTGGTTCAG 
AGGTTGCACT CCTCCTTTTC ACTAACGTGC ACTGGTGAGA GCGAACTGAG CTGGCAGCAC 
CCCCATTCCG AATCTGAACT GGGGAATGTA GTCATTGGAA GTGAAGAAAA CAACAGCGGC 
CTCTTTGTGT CTGTTCTGGA AGTGACCAAC GCTTCCGCCT CCAATACGGG CCTCTACACC 
TGCTACTACA ATCACACCCA GACTGAGGAC AGTGAGATTG AAGGCACTGA TATCTACATC 
TATGTCCCAG ACACCAATGT CCCTTTTGTT CCCCCCGGGT TGTTTGACCA TATCATTGTG 
GTTGAGGAAG ATGAATCTGC TTTCATACCT TGTCGCACTA CCGACCCTTC CAGCCAAGTG 
ACCCTAAAAA GTATCGAAAA TTCTAAGACT GTCAGTGCCT TTTATGACAG CAAGCAAGGA 
TTTGTTGGAA ACTTCCAACC AGGATCATAC ATCTGTGAAA CCGTCTCTAA CAAAATGGTC 
TACCAGACAG ACCTTTACAT TCTACAGACA TGGAAAGCAA CGCACAACAT TTCCGTTGAG 
ATGGAAGCCC CCCAAACTGT ATTTCGAACT GGGGAAACCA TTACCATTTC CTGCATTGTG 
CTGGAGAATG AGGTGGTAGA ATTGAAGTGG TACTACCCTG GACAACAGAG AGGCGTTGGC 
AAAAGAAATG TTGAAGAAAG CAGGGTTCCA TCTCAGAGAC TGGTGTACAC CTTAACCATT 
GCAAATGCCA CTGCGGATGA CTCAGGAGAG TACCAGTGTG CCGTTCTACA TGCAACACTA 
GATAACAAGG TCATCAAGAA GACAAATATA ACAGTTCATG AAAAGGGATT CATCAATCTG 
GAGCCCATGT TTGGATCTGA GGAATATGCC AACCTCCATG AAGTGAAGAG CTTTATAGTA 
GACTTGCATG CCTATCCCTG CCCTAGGGTC TTCTGGTTGA AGGACAACCG GACACTGAGT 
GAAAATCTGA CAGAGATCAC GACTAGCACT GTTACAACTA AAGAAACCAG GTTTCAGAGT 
AAACTTAAAC TGATCCGGGC CAAAGAAGAA GACAGCGGAC TGTACACTCT TGTGGCACAA 
AATGACGCAG AAACTAAAAG CTACTCGTTT ATTTTGCAGA TAAAAGTACC CGCTTTGATA 
TTGGAACTGG TTGATGAACA TCATGGGACA TCTGGTGAAC AGACTGTTGG GTGCTTAGCC 
AAAGGGATGC CGGTCCCTGA TGTGGAATGG TTGGTCTGCA AAGACATCAA AAGATGCAAC 
AATGACACCT TGTGGTCCCT TCTGGCAATA AATGGGTCAG AAATCAGTAT GGAGACCCAT 
CAAGATTATG AGAACACAAT AGAAAGCCAG GTGACCTTTA AGAAAGTTGA GGAGACAATG 
GCAATCCGAT GTATCGCAAG AAACGACCTC GGTGTGGTGG CCAGGGAACT AAAGCTGGTT 
GCACCCACCC TACGTTCTGA ACTAACTGTG GCAGCCGCCG TCCTTGTCCT TCTGGTGATT 
GTCATCATAT CTCTCATTGT ACTGGTCATC ATATGGAAAC AGAAACCAAG GTATGAAATA 
AGATGGCGAG TGATCGAGTC CATCAGCCCA GATGGCCATG AATATATCTA TGTGGACCCA 
ATGCAGCTTC CTTATGACTC TCGGTGGGAG TTCCAAAGGG ATGGACTTGT TCTTGGTCGC 
ATCCTTGGCT CTGGGGCTTT TGGGAAAGTG GTTGAAGGCA CAGCATATGG GTTAAGTCGT 
TCTCAGCCAG TTATGAAAGT TGCTGTAAAA ATGTTGAAAC CTACTGCCCG GTCAAGCGAA 
AAGCAGGCAC TGATGTCTGA ACTGAAGATC ATGACACACC TTGGCGCACA TCTCAATATA 
GTCAATTTAT TAGGAGCCTG TACTAAGTCA GGACCAATTT ATATCATAAC TGAATACTGC 
TTCTATGGTG ACCTCGTGAA TTACCTGCAT AAGAACCGAG ATAATTTTCA GAGCCGACAC 
CCAGAGAAAT CAAAGAAGGA TTTGGACATC TTTGGGCTGA ACCCAGCTGA CGAGAGTACG 
AGAAGCTATG TTATCTTATC ATTTGAAAAC AACGGCGACT ATATGGACAT GAAACAAGCT 
GACACCATGC AGTATGTACC CATGCTGGAA ATGAAAGAAC CCTCCAAGTA TTCAGATATC 
CAGAGATCCT TGTATGACCG GCCTGCGTCG TACAAGAAGA AGCCTTTGTC AGAAGTGAAA 
AATATCTTGT CTGATGATGG ATTCGATGGA TTCGATGGTC TAACAGTACT GGACTTGCTC 
AGTTTCACAT ATCAAGTTGC AAGGGGCATG GAATTCTTAG CCTCTAAAAA TTGTGTACAT 
CGGGATTTGG CAGCTCGGAA TGTCCTATTA GCACATGGGA AAATTGTAAA AATCTGTGAC 
TTTGGCCTAG CAAGAGACAT CATGCACGAC TCCAACTATG TATCCAAAGG CAGCACTTTC 
CTTCCAGTAA AGTGGATGGC GCCCGAAAGC ATCTTCGATA ACCTGTACAC AACTCTCAGC 
GATGTGTGGT CATTTGGAAT CCTGCTGTGG GAAATATTTT CTCTTGGGGG CACACCGTAT 
CCTGGCATGA TTGTGGATTC AACCTTCTAT AATAAGATAA AAAGTGGCTA CAGAATGGCA 
AAACCTGACC ATGCCACCCA TGAAGTGTAC GACATCATGG TAAAATGTTG GAACAGTGAA 
CCAGAAAAAA GGCCATCATT TCGCCATCTG AGTGACATTG TGGAAAGTCT ACTTCCTATG 
GAATACAAAA GGTGCTACGA GACAGTTCTT CATGATTTCC TTAAAAGTGA CCACCCTGCA 
GTTACTCGCA TGAGAAGTGA CAGTGACAAT TCCTACATTG GGGTGACGTA TAAGAATGAA 
AACAAAATGA AGGACAGAGA GAGTGGGTTT GATGAGCAAA GGCTGAGCGC TGACAGCGGT 
TACATTATTC CCCTTCCTGA TATTGATCCC GTTTCAGAAG ATGAGTCCAG CAAGAGGAAC 
AGGCACAGTT CTCAAACATC AGAAGAAAGT GCAATCGAGA CCGGGTCCAG CAGTTCCACT 
TTTATAAAGA GGGAGGACGA GACCATTGAA GACATTGAGA TGATGGACGA CATTGGGATC 
GATTCCTCGG ATCTAGTGGA GGACAGTTTC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096290.1
CDS	185..3460
Translation	MMPTTRASLVLGCLLIIGPWVILAQNPLPSIFPEKDELVQRLHSSFSLTCTGESELSWQHPHSESELGNVVIGSEENNSGLFVSVLEVTNASASNTGLYTCYYNHTQTEDSEIEGTDIYIYVPDTNVPFVPPGLFDHIIVVEEDESAFIPCRTTDPSSQVTLKSIENSKTVSAFYDSKQGFVGNFQPGSYICETVSNKMVYQTDLYILQTWKATHNISVEMEAPQTVFRTGETITISCIVLENEVVELKWYYPGQQRGVGKRNVEESRVPSQRLVYTLTIANATADDSGEYQCAVLHATLDNKVIKKTNITVHEKGFINLEPMFGSEEYANLHEVKSFIVDLHAYPCPRVFWLKDNRTLSENLTEITTSTVTTKETRFQSKLKLIRAKEEDSGLYTLVAQNDAETKSYSFILQIKVPALILELVDEHHGTSGEQTVGCLAKGMPVPDVEWLVCKDIKRCNNDTLWSLLAINGSEISMETHQDYENTIESQVTFKKVEETMAIRCIARNDLGVVARELKLVAPTLRSELTVAAAVLVLLVIVIISLIVLVIIWKQKPRYEIRWRVIESISPDGHEYIYVDPMQLPYDSRWEFQRDGLVLGRILGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLGAHLNIVNLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDNFQSRHPEKSKKDLDIFGLNPADESTRSYVILSFENNGDYMDMKQADTMQYVPMLEMKEPSKYSDIQRSLYDRPASYKKKPLSEVKNILSDDGFDGFDGLTVLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAHGKIVKICDFGLARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSFGILLWEIFSLGGTPYPGMIVDSTFYNKIKSGYRMAKPDHATHEVYDIMVKCWNSEPEKRPSFRHLSDIVESLLPMEYKRCYETVLHDFLKSDHPAVTRMRSDSDNSYIGVTYKNENKMKDRESGFDEQRLSADSGYIIPLPDIDPVSEDESSKRNRHSSQTSEESAIETGSSSSTFIKREDETIEDIEMMDDIGIDSSDLVEDSFL

Target ORF information:

RefSeq Version	NM_001102820.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102820.1

ORF Insert Sequence:

ATGATGCCTA CCACGAGGGC ATCCCTTGTT TTGGGGTGTC TCCTGATTAT AGGTCCGTGG 
GTCATTCTTG CTCAAAATCC CCTGCCCTCA ATATTTCCTG AAAAGGATGA GTTGGTTCAG 
AGGTTGCACT CCTCCTTTTC ACTAACGTGC ACTGGTGAGA GCGAACTGAG CTGGCAGCAC 
CCCCATTCCG AATCTGAACT GGGGAATGTA GTCATTGGAA GTGAAGAAAA CAACAGCGGC 
CTCTTTGTGT CTGTTCTGGA AGTGACCAAC GCTTCCGCCT CCAATACGGG CCTCTACACC 
TGCTACTACA ATCACACCCA GACTGAGGAC AGTGAGATTG AAGGCACTGA TATCTACATC 
TATGTCCCAG ACACCAATGT CCCTTTTGTT CCCCCCGGGT TGTTTGACCA TATCATTGTG 
GTTGAGGAAG ATGAATCTGC TTTCATACCT TGTCGCACTA CCGACCCTTC CAGCCAAGTG 
ACCCTAAAAA GTATCGAAAA TTCTAAGACT GTCAGTGCCT TTTATGACAG CAAGCAAGGA 
TTTGTTGGAA ACTTCCAACC AGGATCATAC ATCTGTGAAA CCGTCTCTAA CAAAATGGTC 
TACCAGACAG ACCTTTACAT TCTACAGACA TGGAAAGCAA CGCACAACAT TTCCGTTGAG 
ATGGAAGCCC CCCAAACTGT ATTTCGAACT GGGGAAACCA TTACCATTTC CTGCATTGTG 
CTGGAGAATG AGGTGGTAGA ATTGAAGTGG TACTACCCTG GACAACAGAG AGGCGTTGGC 
AAAAGAAATG TTGAAGAAAG CAGGGTTCCA TCTCAGAGAC TGGTGTACAC CTTAACCATT 
GCAAATGCCA CTGCGGATGA CTCAGGAGAG TACCAGTGTG CCGTTCTACA TGCAACACTA 
GATAACAAGG TCATCAAGAA GACAAATATA ACAGTTCATG AAAAGGGATT CATCAATCTG 
GAGCCCATGT TTGGATCTGA GGAATATGCC AACCTCCATG AAGTGAAGAG CTTTATAGTA 
GACTTGCATG CCTATCCCTG CCCTAGGGTC TTCTGGTTGA AGGACAACCG GACACTGAGT 
GAAAATCTGA CAGAGATCAC GACTAGCACT GTTACAACTA AAGAAACCAG GTTTCAGAGT 
AAACTTAAAC TGATCCGGGC CAAAGAAGAA GACAGCGGAC TGTACACTCT TGTGGCACAA 
AATGACGCAG AAACTAAAAG CTACTCGTTT ATTTTGCAGA TAAAAGTACC CGCTTTGATA 
TTGGAACTGG TTGATGAACA TCATGGGACA TCTGGTGAAC AGACTGTTGG GTGCTTAGCC 
AAAGGGATGC CGGTCCCTGA TGTGGAATGG TTGGTCTGCA AAGACATCAA AAGATGCAAC 
AATGACACCT TGTGGTCCCT TCTGGCAATA AATGGGTCAG AAATCAGTAT GGAGACCCAT 
CAAGATTATG AGAACACAAT AGAAAGCCAG GTGACCTTTA AGAAAGTTGA GGAGACAATG 
GCAATCCGAT GTATCGCAAG AAACGACCTC GGTGTGGTGG CCAGGGAACT AAAGCTGGTT 
GCACCCACCC TACGTTCTGA ACTAACTGTG GCAGCCGCCG TCCTTGTCCT TCTGGTGATT 
GTCATCATAT CTCTCATTGT ACTGGTCATC ATATGGAAAC AGAAACCAAG GTATGAAATA 
AGATGGCGAG TGATCGAGTC CATCAGCCCA GATGGCCATG AATATATCTA TGTGGACCCA 
ATGCAGCTTC CTTATGACTC TCGGTGGGAG TTCCAAAGGG ATGGACTTGT TCTTGGTCGC 
ATCCTTGGCT CTGGGGCTTT TGGGAAAGTG GTTGAAGGCA CAGCATATGG GTTAAGTCGT 
TCTCAGCCAG TTATGAAAGT TGCTGTAAAA ATGTTGAAAC CTACTGCCCG GTCAAGCGAA 
AAGCAGGCAC TGATGTCTGA ACTGAAGATC ATGACACACC TTGGCGCACA TCTCAATATA 
GTCAATTTAT TAGGAGCCTG TACTAAGTCA GGACCAATTT ATATCATAAC TGAATACTGC 
TTCTATGGTG ACCTCGTGAA TTACCTGCAT AAGAACCGAG ATAATTTTCA GAGCCGACAC 
CCAGAGAAAT CAAAGAAGGA TTTGGACATC TTTGGGCTGA ACCCAGCTGA CGAGAGTACG 
AGAAGCTATG TTATCTTATC ATTTGAAAAC AACGGCGACT ATATGGACAT GAAACAAGCT 
GACACCATGC AGTATGTACC CATGCTGGAA ATGAAAGAAC CCTCCAAGTA TTCAGATATC 
CAGAGATCCT TGTATGACCG GCCTGCGTCG TACAAGAAGA AGCCTTTGTC AGAAGTGAAA 
AATATCTTGT CTGATGATGG ATTCGATGGA TTCGATGGTC TAACAGTACT GGACTTGCTC 
AGTTTCACAT ATCAAGTTGC AAGGGGCATG GAATTCTTAG CCTCTAAAAA TTGTGTACAT 
CGGGATTTGG CAGCTCGGAA TGTCCTATTA GCACATGGGA AAATTGTAAA AATCTGTGAC 
TTTGGCCTAG CAAGAGACAT CATGCACGAC TCCAACTATG TATCCAAAGG CAGCACTTTC 
CTTCCAGTAA AGTGGATGGC GCCCGAAAGC ATCTTCGATA ACCTGTACAC AACTCTCAGC 
GATGTGTGGT CATTTGGAAT CCTGCTGTGG GAAATATTTT CTCTTGGGGG CACACCGTAT 
CCTGGCATGA TTGTGGATTC AACCTTCTAT AATAAGATAA AAAGTGGCTA CAGAATGGCA 
AAACCTGACC ATGCCACCCA TGAAGTGTAC GACATCATGG TAAAATGTTG GAACAGTGAA 
CCAGAAAAAA GGCCATCATT TCGCCATCTG AGTGACATTG TGGAAAGTCT ACTTCCTATG 
GAATACAAAA GGTGCTACGA GACAGTTCTT CATGATTTCC TTAAAAGTGA CCACCCTGCA 
GTTACTCGCA TGAGAAGTGA CAGTGACAAT TCCTACATTG GGGTGACGTA TAAGAATGAA 
AACAAAATGA AGGACAGAGA GAGTGGGTTT GATGAGCAAA GGCTGAGCGC TGACAGCGGT 
TACATTATTC CCCTTCCTGA TATTGATCCC GTTTCAGAAG ATGAGTCCAG CAAGAGGAAC 
AGGCACAGTT CTCAAACATC AGAAGAAAGT GCAATCGAGA CCGGGTCCAG CAGTTCCACT 
TTTATAAAGA GGGAGGACGA GACCATTGAA GACATTGAGA TGATGGACGA CATTGGGATC 
GATTCCTCGG ATCTAGTGGA GGACAGTTTC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pdgfra ( XM_012963365.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818819.1 Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), transcript variant X1, mRNA.

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CloneID	OXa30487
Clone ID Related Accession (Same CDS sequence)	XM_012963365.2
Accession Version	XM_012963365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	platelet-derived growth factor receptor alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCTA CCACGAGGGC ATCCCTTGTT TTGGGGTGTC TCCTGATTAT AGGTCCGTGG 
GTCATTCTTG CTCAAAATCC CCTGCCCTCA ATATTTCCTG AAAAGGATGA GTTGGTTCAG 
AGGTTGCACT CCTCCTTTTC ACTAACGTGC ACTGGTGAGA GTGAACTGAG CTGGCAGCAC 
CCCCATTCCG AATCTGAACT GGGGAATGTA GTCATTGGAA GCGAAGAAAA CAACAGCGGC 
CTCTTTGTGT CTGTTCTGGA AGTGACCAAC GCTTCCGCCT CCAATACGGG CCTCTACACC 
TGCTACTACA ATCACACCCA GACTGAGGAC AGTGAGATTG AAGGCACTGA TATCTACATC 
TATGTCCCAG ACACCAATGT CCCTTTTGTT CCCCCCGGGT TGTTTGACCA TATCATTGTG 
GTTGAGGAAG ATGAATCTGC TTTCATACCT TGTCGCACTA CCGACCCTTC CAGCCAAGTG 
ACCCTAAAAA GTATCGAAAA TTCTAAGACT GTCAGTGCCT TTTATGACAG CAAGCAAGGA 
TTTGTTGGAA ACTTCCAACC AGGATCATAC ATCTGTGAAA CCGTCTCTAA CAAAATGGTC 
TACCAGACAG ACCTTTACAT TCTACAGACA TGGAAAGCAA CGCACAACAT TTCCGTTGAG 
ATGGAAGCCC CCCAAACTGT ATTTCGAACT GGGGAAACCA TTACCATTTC CTGCATTGTG 
CTGGAGAATG AGGTGGTAGA ATTGAAGTGG TACTACCCTG GACAACAGAG AGGGGTTGGC 
AAAAGAAATG TTGAAGAAAG CAGGGTTCCA TCTCAGAGAC TGGTGTACAC CTTAACCATT 
GCAAATGCCA CTGCGGATGA CTCAGGAGAG TACCAGTGTG CCGTTCTACA TGCAACACTA 
GATAACAAGG TCATCAAGAA GACAAATATA ACAGTTCATG AAAAGGGATT CATCAATCTG 
GAGCCCATGT TTGGATCTGA GGAATATGCC AACCTCCATG AAGTGAAGAG CTTTATAGTA 
GACTTGCATG CCTATCCCTG CCCTAGGGTC TTCTGGTTGA AGGACAACCG GACACTGAGT 
GAAAATCTGA CAGAGATCAC GACTAGCACT GTTACAACTA AAGAAACCAG GTTTCAGAGT 
AAACTTAAAC TGATCCGGGC CAAAGAAGAA GACAGCGGAC TGTACACTCT TGTGGCACAA 
AATGACGCAG AAAGTAAAAG CTACTCGTTT ATTTTGCAGA TAAAAGTACC CGCTTTGATA 
TTGGAACTGG TTGATGAACA TCATGGGACA TCTGGTGAAC AGACTGTTGG GTGCTTAGCC 
AAAGGGATGC CGGTCCCTGA TGTGGAATGG TTGGTCTGCA AAGACATCAA AAGATGCAAC 
AATGACACCT TGTGGTCCCT TCTGGCAATA AATGGGTCAG AAATCAGTAT GGAGACCCAT 
CAAGATTATG AGAACACAAT AGAAAGCCAG GTGACCTTCA AGAAAGTTGA GGAGACAATG 
GCAATCCGAT GTATCGCAAG AAACGACCTC GGTGTGGTGG CCAGGGAACT AAAGCTGGTT 
GCACCCACCC TACGTTCTGA ACTAACTGTG GCAGCCGCCG TCCTGGTCCT TCTGGTGATT 
GTCATCATAT CTCTCATTGT ACTGGTCATC ATATGGAAAC AGAAACCAAG GTATGAAATA 
AGATGGCGAG TGATCGAGTC CATCAGCCCA GATGGCCATG AATATATCTA TGTGGACCCA 
ATGCAGCTTC CTTATGACTC TCGGTGGGAG TTCCAAAGGG ATGGACTTGT TCTTGGTCGC 
ATCCTTGGCT CTGGGGCTTT TGGGAAAGTG GTTGAAGGCA CAGCATATGG GTTAAGTCGT 
TCTCAGCCAG TTATGAAAGT TGCTGTAAAA ATGTTGAAAC CTACTGCCCG GTCAAGCGAA 
AAGCAGGCAC TGATGTCTGA ACTGAAGATC ATGACACACC TTGGCGCACA TCTCAATATA 
GTCAATTTAT TAGGAGCCTG TACTAAGTCA GGACCAATTT ATATCATAAC TGAATACTGC 
TTCTATGGTG ACCTCGTGAA TTACCTGCAT AAGAACCGAG ATAATTTTCA GAGCCGACAC 
CCAGAGAAAT CAAAGAAGGA TTTGGACATC TTTGGGCTGA ACCCAGCTGA CGAGAGTACG 
AGAAGCTATG TTATCTTATC ATTTGAAAAC AACGGCGACT ATATGGACAT GAAACAGGCT 
GACACCATGC AGTATGTACC CATGCTGGAA ATGAAAGAAC CCTCCAAGTA TTCAGATATC 
CAGAGATCCT TGTATGACCG GCCTGCGTCG TACAAGAAGA AGCCTTTGTC AGAAGTGAAA 
AATATCTTGT CTGATGATGG ATTCGATGGA TTTGATGGTC TAACAGTACT GGACTTGCTC 
AGTTTCACAT ATCAAGTTGC AAGGGGCATG GAATTCTTAG CCTCTAAAAA TTGTGTACAT 
CGGGATTTGG CAGCTCGGAA TGTCCTATTA GCACATGGGA AAATTGTAAA AATCTGTGAC 
TTTGGCCTAG CAAGAGACAT CATGCACGAC TCCAACTATG TATCCAAAGG CAGCACTTTC 
CTTCCAGTAA AGTGGATGGC GCCCGAAAGC ATCTTCGATA ACCTGTACAC AACTCTCAGC 
GATGTGTGGT CATTTGGAAT CCTGCTGTGG GAAATATTTT CTCTTGGGGG CACACCGTAT 
CCTGGCATGA TTGTAGATTC AACCTTCTAT AATAAGATAA AAAGTGGCTA CAGAATGGCA 
AAACCTGACC ATGCCACCCA TGAAGTGTAC GACATCATGG TAAAATGTTG GAACAGTGAA 
CCAGAAAAAA GGCCATCATT TCGCCATCTG AGTGACATTG TGGAAAGTCT ACTTCCTATG 
GAATACAAAA GGTGCTACGA GACAGTTCTT CACGATTTCC TTAAAAGTGA CCACCCTGCA 
GTTACTCGCA TGAGAAGTGA CAGTGACAAT TCCTACATTG GGGTGACGTA TAAGAATGAA 
AACAAAATGA AGGACAGAGA GAGTGGGTTT GATGAGCAAA GGCTGAGCGC TGACAGCGGT 
TACATTATTC CCCTTCCTGA TATTGATCCC GTTTCAGAAG ATGAGTCCAG CAAGAGGAAC 
AGGCACAGTT CTCAAACATC AGAAGAAAGT GCAATCGAGA CCGGGTCCAG CAGTTCCACT 
TTTATAAAGA GGGAGGACGA GACCATTGAA GACATTGAGA TGATGGACGA CATTGGGATC 
GATTCCTCGG ATCTAGTGGA GGACAGTTTC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818819.1
CDS	911..4186
Translation	MMPTTRASLVLGCLLIIGPWVILAQNPLPSIFPEKDELVQRLHSSFSLTCTGESELSWQHPHSESELGNVVIGSEENNSGLFVSVLEVTNASASNTGLYTCYYNHTQTEDSEIEGTDIYIYVPDTNVPFVPPGLFDHIIVVEEDESAFIPCRTTDPSSQVTLKSIENSKTVSAFYDSKQGFVGNFQPGSYICETVSNKMVYQTDLYILQTWKATHNISVEMEAPQTVFRTGETITISCIVLENEVVELKWYYPGQQRGVGKRNVEESRVPSQRLVYTLTIANATADDSGEYQCAVLHATLDNKVIKKTNITVHEKGFINLEPMFGSEEYANLHEVKSFIVDLHAYPCPRVFWLKDNRTLSENLTEITTSTVTTKETRFQSKLKLIRAKEEDSGLYTLVAQNDAESKSYSFILQIKVPALILELVDEHHGTSGEQTVGCLAKGMPVPDVEWLVCKDIKRCNNDTLWSLLAINGSEISMETHQDYENTIESQVTFKKVEETMAIRCIARNDLGVVARELKLVAPTLRSELTVAAAVLVLLVIVIISLIVLVIIWKQKPRYEIRWRVIESISPDGHEYIYVDPMQLPYDSRWEFQRDGLVLGRILGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLGAHLNIVNLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDNFQSRHPEKSKKDLDIFGLNPADESTRSYVILSFENNGDYMDMKQADTMQYVPMLEMKEPSKYSDIQRSLYDRPASYKKKPLSEVKNILSDDGFDGFDGLTVLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAHGKIVKICDFGLARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSFGILLWEIFSLGGTPYPGMIVDSTFYNKIKSGYRMAKPDHATHEVYDIMVKCWNSEPEKRPSFRHLSDIVESLLPMEYKRCYETVLHDFLKSDHPAVTRMRSDSDNSYIGVTYKNENKMKDRESGFDEQRLSADSGYIIPLPDIDPVSEDESSKRNRHSSQTSEESAIETGSSSSTFIKREDETIEDIEMMDDIGIDSSDLVEDSFL

Target ORF information:

RefSeq Version	XM_012963365.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis platelet-derived growth factor receptor, alpha polypeptide (pdgfra), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963365.2

ORF Insert Sequence:

ATGATGCCTA CCACGAGGGC ATCCCTTGTT TTGGGGTGTC TCCTGATTAT AGGTCCGTGG 
GTCATTCTTG CTCAAAATCC CCTGCCCTCA ATATTTCCTG AAAAGGATGA GTTGGTTCAG 
AGGTTGCACT CCTCCTTTTC ACTAACGTGC ACTGGTGAGA GTGAACTGAG CTGGCAGCAC 
CCCCATTCCG AATCTGAACT GGGGAATGTA GTCATTGGAA GCGAAGAAAA CAACAGCGGC 
CTCTTTGTGT CTGTTCTGGA AGTGACCAAC GCTTCCGCCT CCAATACGGG CCTCTACACC 
TGCTACTACA ATCACACCCA GACTGAGGAC AGTGAGATTG AAGGCACTGA TATCTACATC 
TATGTCCCAG ACACCAATGT CCCTTTTGTT CCCCCCGGGT TGTTTGACCA TATCATTGTG 
GTTGAGGAAG ATGAATCTGC TTTCATACCT TGTCGCACTA CCGACCCTTC CAGCCAAGTG 
ACCCTAAAAA GTATCGAAAA TTCTAAGACT GTCAGTGCCT TTTATGACAG CAAGCAAGGA 
TTTGTTGGAA ACTTCCAACC AGGATCATAC ATCTGTGAAA CCGTCTCTAA CAAAATGGTC 
TACCAGACAG ACCTTTACAT TCTACAGACA TGGAAAGCAA CGCACAACAT TTCCGTTGAG 
ATGGAAGCCC CCCAAACTGT ATTTCGAACT GGGGAAACCA TTACCATTTC CTGCATTGTG 
CTGGAGAATG AGGTGGTAGA ATTGAAGTGG TACTACCCTG GACAACAGAG AGGGGTTGGC 
AAAAGAAATG TTGAAGAAAG CAGGGTTCCA TCTCAGAGAC TGGTGTACAC CTTAACCATT 
GCAAATGCCA CTGCGGATGA CTCAGGAGAG TACCAGTGTG CCGTTCTACA TGCAACACTA 
GATAACAAGG TCATCAAGAA GACAAATATA ACAGTTCATG AAAAGGGATT CATCAATCTG 
GAGCCCATGT TTGGATCTGA GGAATATGCC AACCTCCATG AAGTGAAGAG CTTTATAGTA 
GACTTGCATG CCTATCCCTG CCCTAGGGTC TTCTGGTTGA AGGACAACCG GACACTGAGT 
GAAAATCTGA CAGAGATCAC GACTAGCACT GTTACAACTA AAGAAACCAG GTTTCAGAGT 
AAACTTAAAC TGATCCGGGC CAAAGAAGAA GACAGCGGAC TGTACACTCT TGTGGCACAA 
AATGACGCAG AAAGTAAAAG CTACTCGTTT ATTTTGCAGA TAAAAGTACC CGCTTTGATA 
TTGGAACTGG TTGATGAACA TCATGGGACA TCTGGTGAAC AGACTGTTGG GTGCTTAGCC 
AAAGGGATGC CGGTCCCTGA TGTGGAATGG TTGGTCTGCA AAGACATCAA AAGATGCAAC 
AATGACACCT TGTGGTCCCT TCTGGCAATA AATGGGTCAG AAATCAGTAT GGAGACCCAT 
CAAGATTATG AGAACACAAT AGAAAGCCAG GTGACCTTCA AGAAAGTTGA GGAGACAATG 
GCAATCCGAT GTATCGCAAG AAACGACCTC GGTGTGGTGG CCAGGGAACT AAAGCTGGTT 
GCACCCACCC TACGTTCTGA ACTAACTGTG GCAGCCGCCG TCCTGGTCCT TCTGGTGATT 
GTCATCATAT CTCTCATTGT ACTGGTCATC ATATGGAAAC AGAAACCAAG GTATGAAATA 
AGATGGCGAG TGATCGAGTC CATCAGCCCA GATGGCCATG AATATATCTA TGTGGACCCA 
ATGCAGCTTC CTTATGACTC TCGGTGGGAG TTCCAAAGGG ATGGACTTGT TCTTGGTCGC 
ATCCTTGGCT CTGGGGCTTT TGGGAAAGTG GTTGAAGGCA CAGCATATGG GTTAAGTCGT 
TCTCAGCCAG TTATGAAAGT TGCTGTAAAA ATGTTGAAAC CTACTGCCCG GTCAAGCGAA 
AAGCAGGCAC TGATGTCTGA ACTGAAGATC ATGACACACC TTGGCGCACA TCTCAATATA 
GTCAATTTAT TAGGAGCCTG TACTAAGTCA GGACCAATTT ATATCATAAC TGAATACTGC 
TTCTATGGTG ACCTCGTGAA TTACCTGCAT AAGAACCGAG ATAATTTTCA GAGCCGACAC 
CCAGAGAAAT CAAAGAAGGA TTTGGACATC TTTGGGCTGA ACCCAGCTGA CGAGAGTACG 
AGAAGCTATG TTATCTTATC ATTTGAAAAC AACGGCGACT ATATGGACAT GAAACAGGCT 
GACACCATGC AGTATGTACC CATGCTGGAA ATGAAAGAAC CCTCCAAGTA TTCAGATATC 
CAGAGATCCT TGTATGACCG GCCTGCGTCG TACAAGAAGA AGCCTTTGTC AGAAGTGAAA 
AATATCTTGT CTGATGATGG ATTCGATGGA TTTGATGGTC TAACAGTACT GGACTTGCTC 
AGTTTCACAT ATCAAGTTGC AAGGGGCATG GAATTCTTAG CCTCTAAAAA TTGTGTACAT 
CGGGATTTGG CAGCTCGGAA TGTCCTATTA GCACATGGGA AAATTGTAAA AATCTGTGAC 
TTTGGCCTAG CAAGAGACAT CATGCACGAC TCCAACTATG TATCCAAAGG CAGCACTTTC 
CTTCCAGTAA AGTGGATGGC GCCCGAAAGC ATCTTCGATA ACCTGTACAC AACTCTCAGC 
GATGTGTGGT CATTTGGAAT CCTGCTGTGG GAAATATTTT CTCTTGGGGG CACACCGTAT 
CCTGGCATGA TTGTAGATTC AACCTTCTAT AATAAGATAA AAAGTGGCTA CAGAATGGCA 
AAACCTGACC ATGCCACCCA TGAAGTGTAC GACATCATGG TAAAATGTTG GAACAGTGAA 
CCAGAAAAAA GGCCATCATT TCGCCATCTG AGTGACATTG TGGAAAGTCT ACTTCCTATG 
GAATACAAAA GGTGCTACGA GACAGTTCTT CACGATTTCC TTAAAAGTGA CCACCCTGCA 
GTTACTCGCA TGAGAAGTGA CAGTGACAAT TCCTACATTG GGGTGACGTA TAAGAATGAA 
AACAAAATGA AGGACAGAGA GAGTGGGTTT GATGAGCAAA GGCTGAGCGC TGACAGCGGT 
TACATTATTC CCCTTCCTGA TATTGATCCC GTTTCAGAAG ATGAGTCCAG CAAGAGGAAC 
AGGCACAGTT CTCAAACATC AGAAGAAAGT GCAATCGAGA CCGGGTCCAG CAGTTCCACT 
TTTATAAAGA GGGAGGACGA GACCATTGAA GACATTGAGA TGATGGACGA CATTGGGATC 
GATTCCTCGG ATCTAGTGGA GGACAGTTTC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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pdgfra cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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