kif5a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	kif5a
Entrez Gene ID	100124766
Full Name	kinesin family member 5A
Synonyms	d12s1889,my050,nkhc,spg10
General protein information	Preferred Names kinesin family member 5A Names kinesin heavy chain
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012956287.2	XP_012811741.1	kinesin heavy chain isoform X2
XM_012956286.2	XP_012811740.1	kinesin heavy chain isoform X1
NM_001102745.1	NP_001096215.1	kinesin heavy chain
XM_031895597.1	XP_031751457.1	kinesin heavy chain isoform X1
XM_031895598.1	XP_031751458.1	kinesin heavy chain isoform X2
XM_012956286.3	XP_012811740.1	kinesin heavy chain isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIF5A	NP_004975.2
Bos taurus (cattle)	KIF5A	NP_001192623.1
Danio rerio (zebrafish)	kif5aa	NP_001186705.1
Canis lupus familiaris (dog)	KIF5A	XP_531648.2
Mus musculus (house mouse)	Kif5a	NP_032473.2
Xenopus tropicalis (tropical clawed frog)	kif5a	NP_001096215.1
Pan troglodytes (chimpanzee)	KIF5A	XP_509167.3
	KIF5A	XP_002798698.1
Rattus norvegicus (Norway rat)	Kif5a	NP_997688.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following kif5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa30426	XM_012956287.2 Latest version!	Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa30425	XM_012956286.3 Latest version!	Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa51076	XM_031895597.1 Latest version!	Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa51077	XM_031895598.1 Latest version!	Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa24642	NM_001102745.1 Latest version!	Xenopus tropicalis kinesin family member 5A (kif5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa30425	XM_012956286.2	Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kif5a ( XM_012956287.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811741.1 Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa30426
Clone ID Related Accession (Same CDS sequence)	XM_012956287.2
Accession Version	XM_012956287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin heavy chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACT GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAGCTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT AGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCGATGGAAG CGTATGAAGT CAAGAAGGTC 
CTGGAGATGC AGATGGAGAG CCACCGAGAG GCCCATCACA AACAACTGGC TCGTCTCAGG 
GATGAAATTA ACGAGAAACA GAAGATCATT GATGAGCTCA AAGATCTGAA TCAGAAGCTG 
CAGCTGGAGC TGGAAAAGCT TAGAGCTGAT TACGAGAAGC TGAAGAGCGA GGAACATGAG 
AAGAGCCACA AGCTGCAGGA GCTGACATTT CTGTACGAGA AACACGAACA ATCCAAACAG 
GATCTTAAAG GGTTGGAGGA GACAGTCGCC CGTGAACTCC AGACCCTCCA CAACCTTCGG 
AAGCTTTTTG TTCAAGACGT CACATCTCGA GTCAAGAAAA GTGCTGAAAT GGAACCCGAG 
GACAGTGGGG GGATACACTC ACAGAAACAG AAGATTTCCT TTCTTGAGAA CAACCTGGAA 
CAGCTTACAA AGGTTCACAA ACAGCTGGAT GACTGGGTAT CAAAGGTACT GAATGATACT 
AACGCAGAGA TGTCTGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811741.1
CDS	420..3020
Translation	MADNSINTECSIKVLCRFRPLNQSEVQRGDKFLPIFQGGDTVIVGGKPYAFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTASGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYAMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENIETEQKLSGKLYLVDLAGSEKVSKTGAEGSVLDEAKNINKSLSALGNVISALAEGSKGYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKNKTLKETIQKLEAELSRWRNGENVPETEQLSDGEQLTQDTANETPVNDNNSSIVIRISEEERKKYEEEIRKMYKQLDDKDDEINQQSQLVEKLKQQMLDQDELLISTRGDNDKVQMELSRLQSENDSAKEEVKEVLQALEELAVNYDQKSQEVEDKTLQNKILADELSQKVANLMSLENDLHRLQEFSSHQRKRIAEVLNGLMKDLSEFSVIVGNGEFKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMHSVELKKRQLEESYDALSEELAKMQVQENVTEVAIKDKEQDAMEAYEVKKVLEMQMESHREAHHKQLARLRDEINEKQKIIDELKDLNQKLQLELEKLRADYEKLKSEEHEKSHKLQELTFLYEKHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTSRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLDDWVSKVLNDTNAEMSA

Target ORF information:

RefSeq Version	XM_012956287.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956287.2

ORF Insert Sequence:

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACT GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAGCTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT AGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCGATGGAAG CGTATGAAGT CAAGAAGGTC 
CTGGAGATGC AGATGGAGAG CCACCGAGAG GCCCATCACA AACAACTGGC TCGTCTCAGG 
GATGAAATTA ACGAGAAACA GAAGATCATT GATGAGCTCA AAGATCTGAA TCAGAAGCTG 
CAGCTGGAGC TGGAAAAGCT TAGAGCTGAT TACGAGAAGC TGAAGAGCGA GGAACATGAG 
AAGAGCCACA AGCTGCAGGA GCTGACATTT CTGTACGAGA AACACGAACA ATCCAAACAG 
GATCTTAAAG GGTTGGAGGA GACAGTCGCC CGTGAACTCC AGACCCTCCA CAACCTTCGG 
AAGCTTTTTG TTCAAGACGT CACATCTCGA GTCAAGAAAA GTGCTGAAAT GGAACCCGAG 
GACAGTGGGG GGATACACTC ACAGAAACAG AAGATTTCCT TTCTTGAGAA CAACCTGGAA 
CAGCTTACAA AGGTTCACAA ACAGCTGGAT GACTGGGTAT CAAAGGTACT GAATGATACT 
AACGCAGAGA TGTCTGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif5a ( XM_012956286.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811740.1 Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa30425
Clone ID Related Accession (Same CDS sequence)	XM_012956286.2 , XM_012956286.3
Accession Version	XM_012956286.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin heavy chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956286.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACT GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAGCTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT AGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCGATGGAAG CGTATGAAGT CAAGGTCCTG 
GAGATGCAGA TGGAGAGCCA CCGAGAGGCC CATCACAAAC AACTGGCTCG TCTCAGGGAT 
GAAATTAACG AGAAACAGAA GATCATTGAT GAGCTCAAAG ATCTGAATCA GAAGCTGCAG 
CTGGAGCTGG AAAAGCTTAG AGCTGATTAC GAGAAGCTGA AGAGCGAGGA ACATGAGAAG 
AGCCACAAGC TGCAGGAGCT GACATTTCTG TACGAGAAAC ACGAACAATC CAAACAGGAT 
CTTAAAGGGT TGGAGGAGAC AGTCGCCCGT GAACTCCAGA CCCTCCACAA CCTTCGGAAG 
CTTTTTGTTC AAGACGTCAC ATCTCGAGTC AAGAAAAGTG CTGAAATGGA ACCCGAGGAC 
AGTGGGGGGA TACACTCACA GAAACAGAAG ATTTCCTTTC TTGAGAACAA CCTGGAACAG 
CTTACAAAGG TTCACAAACA GTTGGTTCGT GACAATGCAG ATCTTCGTTG TGAGCTTCCT 
AAACTGGAAA AACGTCTTCG GGCTACAGCT GAGAGAGTTA AGGCCCTGGA GGGTGCACTG 
AAGGAGGCCA AGGAAGGAGC TATGAAAGAC AAGAGGCGGT ATCAACAGGA AGTAGATCGT 
ATTAAGGAGG CCGTACGCTA TAAGAACACA GTTAAACGTG CACACACAGC ACAGATTGCA 
AAGCCCATCC GGCCTGGGCA CTACCCTGCA TCCTCACCCA CCAACCCATA TGGCATCCGG 
AATTCTGACT CAATCAGTTA TACCAACAAC CTCTTTCAGA ACTACCAAAA TATATACATG 
CGGGCAACCA GCCCAAGCAG TGAAACATTC TTTGCCAATC CATGTTCCAG CACACGGGGC 
AGTTCTTCCA GTGGCAGCTT GCTTTCCTAT CAGAAGATGA ATGTGGATAA TGGAAATGCT 
ACAGATATCA ATGATAACAG AAGCGATTTG CCATGTGGCT GTGAAGCTGA GGATCAGTCC 
AAGTTGTTCC CTCTTCATCA GGAGACAGCA GCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811740.1
CDS	405..3503
Translation	MADNSINTECSIKVLCRFRPLNQSEVQRGDKFLPIFQGGDTVIVGGKPYAFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTASGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYAMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENIETEQKLSGKLYLVDLAGSEKVSKTGAEGSVLDEAKNINKSLSALGNVISALAEGSKGYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKNKTLKETIQKLEAELSRWRNGENVPETEQLSDGEQLTQDTANETPVNDNNSSIVIRISEEERKKYEEEIRKMYKQLDDKDDEINQQSQLVEKLKQQMLDQDELLISTRGDNDKVQMELSRLQSENDSAKEEVKEVLQALEELAVNYDQKSQEVEDKTLQNKILADELSQKVANLMSLENDLHRLQEFSSHQRKRIAEVLNGLMKDLSEFSVIVGNGEFKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMHSVELKKRQLEESYDALSEELAKMQVQENVTEVAIKDKEQDAMEAYEVKVLEMQMESHREAHHKQLARLRDEINEKQKIIDELKDLNQKLQLELEKLRADYEKLKSEEHEKSHKLQELTFLYEKHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTSRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKNTVKRAHTAQIAKPIRPGHYPASSPTNPYGIRNSDSISYTNNLFQNYQNIYMRATSPSSETFFANPCSSTRGSSSSGSLLSYQKMNVDNGNATDINDNRSDLPCGCEAEDQSKLFPLHQETAAS

Target ORF information:

RefSeq Version	XM_012956286.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956286.3

ORF Insert Sequence:

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACT GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAGCTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT AGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCGATGGAAG CGTATGAAGT CAAGGTCCTG 
GAGATGCAGA TGGAGAGCCA CCGAGAGGCC CATCACAAAC AACTGGCTCG TCTCAGGGAT 
GAAATTAACG AGAAACAGAA GATCATTGAT GAGCTCAAAG ATCTGAATCA GAAGCTGCAG 
CTGGAGCTGG AAAAGCTTAG AGCTGATTAC GAGAAGCTGA AGAGCGAGGA ACATGAGAAG 
AGCCACAAGC TGCAGGAGCT GACATTTCTG TACGAGAAAC ACGAACAATC CAAACAGGAT 
CTTAAAGGGT TGGAGGAGAC AGTCGCCCGT GAACTCCAGA CCCTCCACAA CCTTCGGAAG 
CTTTTTGTTC AAGACGTCAC ATCTCGAGTC AAGAAAAGTG CTGAAATGGA ACCCGAGGAC 
AGTGGGGGGA TACACTCACA GAAACAGAAG ATTTCCTTTC TTGAGAACAA CCTGGAACAG 
CTTACAAAGG TTCACAAACA GTTGGTTCGT GACAATGCAG ATCTTCGTTG TGAGCTTCCT 
AAACTGGAAA AACGTCTTCG GGCTACAGCT GAGAGAGTTA AGGCCCTGGA GGGTGCACTG 
AAGGAGGCCA AGGAAGGAGC TATGAAAGAC AAGAGGCGGT ATCAACAGGA AGTAGATCGT 
ATTAAGGAGG CCGTACGCTA TAAGAACACA GTTAAACGTG CACACACAGC ACAGATTGCA 
AAGCCCATCC GGCCTGGGCA CTACCCTGCA TCCTCACCCA CCAACCCATA TGGCATCCGG 
AATTCTGACT CAATCAGTTA TACCAACAAC CTCTTTCAGA ACTACCAAAA TATATACATG 
CGGGCAACCA GCCCAAGCAG TGAAACATTC TTTGCCAATC CATGTTCCAG CACACGGGGC 
AGTTCTTCCA GTGGCAGCTT GCTTTCCTAT CAGAAGATGA ATGTGGATAA TGGAAATGCT 
ACAGATATCA ATGATAACAG AAGCGATTTG CCATGTGGCT GTGAAGCTGA GGATCAGTCC 
AAGTTGTTCC CTCTTCATCA GGAGACAGCA GCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif5a ( XM_031895597.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751457.1 Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa51076
Clone ID Related Accession (Same CDS sequence)	XM_031895597.1
Accession Version	XM_031895597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin heavy chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACT GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAGCTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT AGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCGATGGAAG CGTATGAAGT CAAGAAGGTC 
CTGGAGATGC AGATGGAGAG CCACCGAGAG GCCCATCACA AACAACTGGC TCGTCTCAGG 
GATGAAATTA ACGAGAAACA GAAGATCATT GATGAGCTCA AAGATCTGAA TCAGAAGCTG 
CAGCTGGAGC TGGAAAAGCT TAGAGCTGAT TACGAGAAGC TGAAGAGCGA GGAACATGAG 
AAGAGCCACA AGCTGCAGGA GCTGACATTT CTGTACGAGA AACACGAACA ATCCAAACAG 
GATCTTAAAG GGTTGGAGGA GACAGTCGCC CGTGAACTCC AGACCCTCCA CAACCTTCGG 
AAGCTTTTTG TTCAAGACGT CACATCTCGA GTCAAGAAAA GTGCTGAAAT GGAACCCGAG 
GACAGTGGGG GGATACACTC ACAGAAACAG AAGATTTCCT TTCTTGAGAA CAACCTGGAA 
CAGCTTACAA AGGTTCACAA ACAGCTGGAT GACTGGGTAT CAAAGTTGGT TCGTGACAAT 
GCAGATCTTC GTTGTGAGCT TCCTAAACTG GAAAAACGTC TTCGGGCTAC AGCTGAGAGA 
GTTAAGGCCC TGGAGGGTGC ACTGAAGGAG GCCAAGGAAG GAGCTATGAA AGACAAGAGG 
CGGTATCAAC AGGAAGTAGA TCGTATTAAG GAGGCCGTAC GCTATAAGAA CACAGTTAAA 
CGTGCACACA CAGCACAGAT TGCAAAGCCC ATCCGGCCTG GGCACTACCC TGCATCCTCA 
CCCACCAACC CATATGGCAT CCGGAATTCT GACTCAATCA GTTATACCAA CAACCTCTTT 
CAGAACTACC AAAATATATA CATGCGGGCA ACCAGCCCAA GCAGTGAAAC ATTCTTTGCC 
AATCCATGTT CCAGCACACG GGGCAGTTCT TCCAGTGGCA GCTTGCTTTC CTATCAGAAG 
ATGAATGTGG ATAATGGAAA TGCTACAGAT ATCAATGATA ACAGAAGCGA TTTGCCATGT 
GGCTGTGAAG CTGAGGATCA GTCCAAGTTG TTCCCTCTTC ATCAGGAGAC AGCAGCCAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751457.1
CDS	405..3527
Translation	MADNSINTECSIKVLCRFRPLNQSEVQRGDKFLPIFQGGDTVIVGGKPYAFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTASGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYAMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENIETEQKLSGKLYLVDLAGSEKVSKTGAEGSVLDEAKNINKSLSALGNVISALAEGSKGYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKNKTLKETIQKLEAELSRWRNGENVPETEQLSDGEQLTQDTANETPVNDNNSSIVIRISEEERKKYEEEIRKMYKQLDDKDDEINQQSQLVEKLKQQMLDQDELLISTRGDNDKVQMELSRLQSENDSAKEEVKEVLQALEELAVNYDQKSQEVEDKTLQNKILADELSQKVANLMSLENDLHRLQEFSSHQRKRIAEVLNGLMKDLSEFSVIVGNGEFKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMHSVELKKRQLEESYDALSEELAKMQVQENVTEVAIKDKEQDAMEAYEVKKVLEMQMESHREAHHKQLARLRDEINEKQKIIDELKDLNQKLQLELEKLRADYEKLKSEEHEKSHKLQELTFLYEKHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTSRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLDDWVSKLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKNTVKRAHTAQIAKPIRPGHYPASSPTNPYGIRNSDSISYTNNLFQNYQNIYMRATSPSSETFFANPCSSTRGSSSSGSLLSYQKMNVDNGNATDINDNRSDLPCGCEAEDQSKLFPLHQETAAS

Target ORF information:

RefSeq Version	XM_031895597.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895597.1

ORF Insert Sequence:

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACT GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAGCTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT AGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCGATGGAAG CGTATGAAGT CAAGAAGGTC 
CTGGAGATGC AGATGGAGAG CCACCGAGAG GCCCATCACA AACAACTGGC TCGTCTCAGG 
GATGAAATTA ACGAGAAACA GAAGATCATT GATGAGCTCA AAGATCTGAA TCAGAAGCTG 
CAGCTGGAGC TGGAAAAGCT TAGAGCTGAT TACGAGAAGC TGAAGAGCGA GGAACATGAG 
AAGAGCCACA AGCTGCAGGA GCTGACATTT CTGTACGAGA AACACGAACA ATCCAAACAG 
GATCTTAAAG GGTTGGAGGA GACAGTCGCC CGTGAACTCC AGACCCTCCA CAACCTTCGG 
AAGCTTTTTG TTCAAGACGT CACATCTCGA GTCAAGAAAA GTGCTGAAAT GGAACCCGAG 
GACAGTGGGG GGATACACTC ACAGAAACAG AAGATTTCCT TTCTTGAGAA CAACCTGGAA 
CAGCTTACAA AGGTTCACAA ACAGCTGGAT GACTGGGTAT CAAAGTTGGT TCGTGACAAT 
GCAGATCTTC GTTGTGAGCT TCCTAAACTG GAAAAACGTC TTCGGGCTAC AGCTGAGAGA 
GTTAAGGCCC TGGAGGGTGC ACTGAAGGAG GCCAAGGAAG GAGCTATGAA AGACAAGAGG 
CGGTATCAAC AGGAAGTAGA TCGTATTAAG GAGGCCGTAC GCTATAAGAA CACAGTTAAA 
CGTGCACACA CAGCACAGAT TGCAAAGCCC ATCCGGCCTG GGCACTACCC TGCATCCTCA 
CCCACCAACC CATATGGCAT CCGGAATTCT GACTCAATCA GTTATACCAA CAACCTCTTT 
CAGAACTACC AAAATATATA CATGCGGGCA ACCAGCCCAA GCAGTGAAAC ATTCTTTGCC 
AATCCATGTT CCAGCACACG GGGCAGTTCT TCCAGTGGCA GCTTGCTTTC CTATCAGAAG 
ATGAATGTGG ATAATGGAAA TGCTACAGAT ATCAATGATA ACAGAAGCGA TTTGCCATGT 
GGCTGTGAAG CTGAGGATCA GTCCAAGTTG TTCCCTCTTC ATCAGGAGAC AGCAGCCAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif5a ( XM_031895598.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751458.1 Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa51077
Clone ID Related Accession (Same CDS sequence)	XM_031895598.1
Accession Version	XM_031895598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin heavy chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACT GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAGCTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT AGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCGATGGAAG CGTATGAAGT CAAGGTCCTG 
GAGATGCAGA TGGAGAGCCA CCGAGAGGCC CATCACAAAC AACTGGCTCG TCTCAGGGAT 
GAAATTAACG AGAAACAGAA GATCATTGAT GAGCTCAAAG ATCTGAATCA GAAGCTGCAG 
CTGGAGCTGG AAAAGCTTAG AGCTGATTAC GAGAAGCTGA AGAGCGAGGA ACATGAGAAG 
AGCCACAAGC TGCAGGAGCT GACATTTCTG TACGAGAAAC ACGAACAATC CAAACAGGAT 
CTTAAAGGGT TGGAGGAGAC AGTCGCCCGT GAACTCCAGA CCCTCCACAA CCTTCGGAAG 
CTTTTTGTTC AAGACGTCAC ATCTCGAGTC AAGAAAAGTG CTGAAATGGA ACCCGAGGAC 
AGTGGGGGGA TACACTCACA GAAACAGAAG ATTTCCTTTC TTGAGAACAA CCTGGAACAG 
CTTACAAAGG TTCACAAACA GCTGGATGAC TGGGTATCAA AGTTGGTTCG TGACAATGCA 
GATCTTCGTT GTGAGCTTCC TAAACTGGAA AAACGTCTTC GGGCTACAGC TGAGAGAGTT 
AAGGCCCTGG AGGGTGCACT GAAGGAGGCC AAGGAAGGAG CTATGAAAGA CAAGAGGCGG 
TATCAACAGG AAGTAGATCG TATTAAGGAG GCCGTACGCT ATAAGAACAC AGTTAAACGT 
GCACACACAG CACAGATTGC AAAGCCCATC CGGCCTGGGC ACTACCCTGC ATCCTCACCC 
ACCAACCCAT ATGGCATCCG GAATTCTGAC TCAATCAGTT ATACCAACAA CCTCTTTCAG 
AACTACCAAA ATATATACAT GCGGGCAACC AGCCCAAGCA GTGAAACATT CTTTGCCAAT 
CCATGTTCCA GCACACGGGG CAGTTCTTCC AGTGGCAGCT TGCTTTCCTA TCAGAAGATG 
AATGTGGATA ATGGAAATGC TACAGATATC AATGATAACA GAAGCGATTT GCCATGTGGC 
TGTGAAGCTG AGGATCAGTC CAAGTTGTTC CCTCTTCATC AGGAGACAGC AGCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751458.1
CDS	405..3524
Translation	MADNSINTECSIKVLCRFRPLNQSEVQRGDKFLPIFQGGDTVIVGGKPYAFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTASGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYAMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENIETEQKLSGKLYLVDLAGSEKVSKTGAEGSVLDEAKNINKSLSALGNVISALAEGSKGYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKNKTLKETIQKLEAELSRWRNGENVPETEQLSDGEQLTQDTANETPVNDNNSSIVIRISEEERKKYEEEIRKMYKQLDDKDDEINQQSQLVEKLKQQMLDQDELLISTRGDNDKVQMELSRLQSENDSAKEEVKEVLQALEELAVNYDQKSQEVEDKTLQNKILADELSQKVANLMSLENDLHRLQEFSSHQRKRIAEVLNGLMKDLSEFSVIVGNGEFKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMHSVELKKRQLEESYDALSEELAKMQVQENVTEVAIKDKEQDAMEAYEVKVLEMQMESHREAHHKQLARLRDEINEKQKIIDELKDLNQKLQLELEKLRADYEKLKSEEHEKSHKLQELTFLYEKHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTSRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLDDWVSKLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKNTVKRAHTAQIAKPIRPGHYPASSPTNPYGIRNSDSISYTNNLFQNYQNIYMRATSPSSETFFANPCSSTRGSSSSGSLLSYQKMNVDNGNATDINDNRSDLPCGCEAEDQSKLFPLHQETAAS

Target ORF information:

RefSeq Version	XM_031895598.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895598.1

ORF Insert Sequence:

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACT GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAGCTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT AGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCGATGGAAG CGTATGAAGT CAAGGTCCTG 
GAGATGCAGA TGGAGAGCCA CCGAGAGGCC CATCACAAAC AACTGGCTCG TCTCAGGGAT 
GAAATTAACG AGAAACAGAA GATCATTGAT GAGCTCAAAG ATCTGAATCA GAAGCTGCAG 
CTGGAGCTGG AAAAGCTTAG AGCTGATTAC GAGAAGCTGA AGAGCGAGGA ACATGAGAAG 
AGCCACAAGC TGCAGGAGCT GACATTTCTG TACGAGAAAC ACGAACAATC CAAACAGGAT 
CTTAAAGGGT TGGAGGAGAC AGTCGCCCGT GAACTCCAGA CCCTCCACAA CCTTCGGAAG 
CTTTTTGTTC AAGACGTCAC ATCTCGAGTC AAGAAAAGTG CTGAAATGGA ACCCGAGGAC 
AGTGGGGGGA TACACTCACA GAAACAGAAG ATTTCCTTTC TTGAGAACAA CCTGGAACAG 
CTTACAAAGG TTCACAAACA GCTGGATGAC TGGGTATCAA AGTTGGTTCG TGACAATGCA 
GATCTTCGTT GTGAGCTTCC TAAACTGGAA AAACGTCTTC GGGCTACAGC TGAGAGAGTT 
AAGGCCCTGG AGGGTGCACT GAAGGAGGCC AAGGAAGGAG CTATGAAAGA CAAGAGGCGG 
TATCAACAGG AAGTAGATCG TATTAAGGAG GCCGTACGCT ATAAGAACAC AGTTAAACGT 
GCACACACAG CACAGATTGC AAAGCCCATC CGGCCTGGGC ACTACCCTGC ATCCTCACCC 
ACCAACCCAT ATGGCATCCG GAATTCTGAC TCAATCAGTT ATACCAACAA CCTCTTTCAG 
AACTACCAAA ATATATACAT GCGGGCAACC AGCCCAAGCA GTGAAACATT CTTTGCCAAT 
CCATGTTCCA GCACACGGGG CAGTTCTTCC AGTGGCAGCT TGCTTTCCTA TCAGAAGATG 
AATGTGGATA ATGGAAATGC TACAGATATC AATGATAACA GAAGCGATTT GCCATGTGGC 
TGTGAAGCTG AGGATCAGTC CAAGTTGTTC CCTCTTCATC AGGAGACAGC AGCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif5a ( NM_001102745.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001096215.1 Xenopus tropicalis kinesin family member 5A (kif5a), mRNA.

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CloneID	OXa24642
Clone ID Related Accession (Same CDS sequence)	NM_001102745.1
Accession Version	NM_001102745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin heavy chain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC136117.1. ##Evidence-Data-START## Transcript exon combination :: BC136117.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028295, SAMD00028297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACC GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAACTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT GGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCAATGGAAG CGTATGAAGT CAAGAAGGTC 
CTGGAGATGC AGATGGAGAG CCACCGAGAG GCCCATCACA AACAACTGGC TCGTCTCAGG 
GATGAAATTA ACGAGAAACA GAAGATCATT GATGAGCTCA AAGATCTGAA TCAGAAGCTG 
CAGCTGGAGC TGGAAAAGCT TAGAGCTGAT TACGAGAAGC TGAAGAGCGA GGAACATGAG 
AAGAGCCACA AGCTGCAGGA GCTGACATTT CTGTACGAGA AACACGAACA ATCCAAACAG 
GATCTTAAAG GGTTGGAGGA GACAGTCGCC CGTGAACTCC AGACCCTCCA CAACCTTCGG 
AAGCTTTTTG TTCAAGACGT CACATCTCGA GTCAAGAAAA GTGCTGAAAT GGAACCCGAG 
GACAGTGGGG GGATACACTC ACAGAAACAG AAGATTTCCT TTCTTGAGAA CAACCTGGAA 
CAGCTTACAA AGGTTCACAA ACAGTTGGTT CGTGACAATG CAGATCTTCG TTGTGAGCTT 
CCTAAACTGG AAAAACGTCT TCGGGCTACA GCTGAGAGAG TTAAGGCCCT GGAGGGTGCA 
CTGAAGGAGG CCAAGGAAGG AGCTATGAAA GACAAGAGGC GGTATCAACA GGAAGTAGAT 
CGTATTAAGG AGGCCGTACG CTATAAGAAC ACAGTTAAAC GTGCACACAC AGCACAGATT 
GCAAAGCCCA TCCGGCCTGG GCACTACCCT GCATCCTCAC CCACCAACCC ATATGGCATC 
CGGAATTCTG ACTCAATCAG TTATACCAAC AACCTCTTTC AGAACTACCA AAATATATAC 
ATGCGGGCAA CCAGCCCAAG CAGTGAAACA TTCTTTGCCA ATCCATGTTC CAGCACACGG 
GGCAGTTCTT CCAGTGGCAG CTTGCTTTCC TATCAGAAGA TGAATGTGGA TAATGGAAAT 
GCTACAGATA TCAATGATAA CAGAAGCGAT TTGCCATGTG GCTGTGAAGC TGAGGATCAG 
TCCAAGTTGT TCCCTCTTCA TCAGGAGACA GCAGCCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096215.1
CDS	164..3265
Translation	MADNSINTECSIKVLCRFRPLNQSEVQRGDKFLPIFQGGDTVIVGGKPYAFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTASGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYAMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENIETEQKLSGKLYLVDLAGSEKVSKTGAEGSVLDEAKNINKSLSALGNVISALAEGSKGYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKNKTLKETIQKLEAELSRWRNGENVPETEQLSDGEQLTQDTANETPVNDNNSSIVIRISEEERKKYEEEIRKMYKQLDDKDDEINQQSQLVEKLKQQMLDQDELLISTRGDNDKVQMELSRLQSENDSAKEEVKEVLQALEELAVNYDQKSQEVEDKTLQNKILADELSQKVANLMSLENDLHRLQEFSSHQRKRIAEVLNGLMKDLSEFSVIVGNGEFKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMHSVELKKRQLEESYDALSEELAKMQVQENVTEVAIKDKEQDAMEAYEVKKVLEMQMESHREAHHKQLARLRDEINEKQKIIDELKDLNQKLQLELEKLRADYEKLKSEEHEKSHKLQELTFLYEKHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTSRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKNTVKRAHTAQIAKPIRPGHYPASSPTNPYGIRNSDSISYTNNLFQNYQNIYMRATSPSSETFFANPCSSTRGSSSSGSLLSYQKMNVDNGNATDINDNRSDLPCGCEAEDQSKLFPLHQETAAS

Target ORF information:

RefSeq Version	NM_001102745.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 5A (kif5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102745.1

ORF Insert Sequence:

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACC GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAACTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT GGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCAATGGAAG CGTATGAAGT CAAGAAGGTC 
CTGGAGATGC AGATGGAGAG CCACCGAGAG GCCCATCACA AACAACTGGC TCGTCTCAGG 
GATGAAATTA ACGAGAAACA GAAGATCATT GATGAGCTCA AAGATCTGAA TCAGAAGCTG 
CAGCTGGAGC TGGAAAAGCT TAGAGCTGAT TACGAGAAGC TGAAGAGCGA GGAACATGAG 
AAGAGCCACA AGCTGCAGGA GCTGACATTT CTGTACGAGA AACACGAACA ATCCAAACAG 
GATCTTAAAG GGTTGGAGGA GACAGTCGCC CGTGAACTCC AGACCCTCCA CAACCTTCGG 
AAGCTTTTTG TTCAAGACGT CACATCTCGA GTCAAGAAAA GTGCTGAAAT GGAACCCGAG 
GACAGTGGGG GGATACACTC ACAGAAACAG AAGATTTCCT TTCTTGAGAA CAACCTGGAA 
CAGCTTACAA AGGTTCACAA ACAGTTGGTT CGTGACAATG CAGATCTTCG TTGTGAGCTT 
CCTAAACTGG AAAAACGTCT TCGGGCTACA GCTGAGAGAG TTAAGGCCCT GGAGGGTGCA 
CTGAAGGAGG CCAAGGAAGG AGCTATGAAA GACAAGAGGC GGTATCAACA GGAAGTAGAT 
CGTATTAAGG AGGCCGTACG CTATAAGAAC ACAGTTAAAC GTGCACACAC AGCACAGATT 
GCAAAGCCCA TCCGGCCTGG GCACTACCCT GCATCCTCAC CCACCAACCC ATATGGCATC 
CGGAATTCTG ACTCAATCAG TTATACCAAC AACCTCTTTC AGAACTACCA AAATATATAC 
ATGCGGGCAA CCAGCCCAAG CAGTGAAACA TTCTTTGCCA ATCCATGTTC CAGCACACGG 
GGCAGTTCTT CCAGTGGCAG CTTGCTTTCC TATCAGAAGA TGAATGTGGA TAATGGAAAT 
GCTACAGATA TCAATGATAA CAGAAGCGAT TTGCCATGTG GCTGTGAAGC TGAGGATCAG 
TCCAAGTTGT TCCCTCTTCA TCAGGAGACA GCAGCCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif5a ( XM_012956286.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811740.1 Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X1, mRNA.

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CloneID	OXa30425
Clone ID Related Accession (Same CDS sequence)	XM_012956286.2 , XM_012956286.3
Accession Version	XM_012956286.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin heavy chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACT GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAGCTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT AGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCGATGGAAG CGTATGAAGT CAAGGTCCTG 
GAGATGCAGA TGGAGAGCCA CCGAGAGGCC CATCACAAAC AACTGGCTCG TCTCAGGGAT 
GAAATTAACG AGAAACAGAA GATCATTGAT GAGCTCAAAG ATCTGAATCA GAAGCTGCAG 
CTGGAGCTGG AAAAGCTTAG AGCTGATTAC GAGAAGCTGA AGAGCGAGGA ACATGAGAAG 
AGCCACAAGC TGCAGGAGCT GACATTTCTG TACGAGAAAC ACGAACAATC CAAACAGGAT 
CTTAAAGGGT TGGAGGAGAC AGTCGCCCGT GAACTCCAGA CCCTCCACAA CCTTCGGAAG 
CTTTTTGTTC AAGACGTCAC ATCTCGAGTC AAGAAAAGTG CTGAAATGGA ACCCGAGGAC 
AGTGGGGGGA TACACTCACA GAAACAGAAG ATTTCCTTTC TTGAGAACAA CCTGGAACAG 
CTTACAAAGG TTCACAAACA GTTGGTTCGT GACAATGCAG ATCTTCGTTG TGAGCTTCCT 
AAACTGGAAA AACGTCTTCG GGCTACAGCT GAGAGAGTTA AGGCCCTGGA GGGTGCACTG 
AAGGAGGCCA AGGAAGGAGC TATGAAAGAC AAGAGGCGGT ATCAACAGGA AGTAGATCGT 
ATTAAGGAGG CCGTACGCTA TAAGAACACA GTTAAACGTG CACACACAGC ACAGATTGCA 
AAGCCCATCC GGCCTGGGCA CTACCCTGCA TCCTCACCCA CCAACCCATA TGGCATCCGG 
AATTCTGACT CAATCAGTTA TACCAACAAC CTCTTTCAGA ACTACCAAAA TATATACATG 
CGGGCAACCA GCCCAAGCAG TGAAACATTC TTTGCCAATC CATGTTCCAG CACACGGGGC 
AGTTCTTCCA GTGGCAGCTT GCTTTCCTAT CAGAAGATGA ATGTGGATAA TGGAAATGCT 
ACAGATATCA ATGATAACAG AAGCGATTTG CCATGTGGCT GTGAAGCTGA GGATCAGTCC 
AAGTTGTTCC CTCTTCATCA GGAGACAGCA GCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811740.1
CDS	414..3512
Translation	MADNSINTECSIKVLCRFRPLNQSEVQRGDKFLPIFQGGDTVIVGGKPYAFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTASGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYAMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENIETEQKLSGKLYLVDLAGSEKVSKTGAEGSVLDEAKNINKSLSALGNVISALAEGSKGYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKNKTLKETIQKLEAELSRWRNGENVPETEQLSDGEQLTQDTANETPVNDNNSSIVIRISEEERKKYEEEIRKMYKQLDDKDDEINQQSQLVEKLKQQMLDQDELLISTRGDNDKVQMELSRLQSENDSAKEEVKEVLQALEELAVNYDQKSQEVEDKTLQNKILADELSQKVANLMSLENDLHRLQEFSSHQRKRIAEVLNGLMKDLSEFSVIVGNGEFKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMHSVELKKRQLEESYDALSEELAKMQVQENVTEVAIKDKEQDAMEAYEVKVLEMQMESHREAHHKQLARLRDEINEKQKIIDELKDLNQKLQLELEKLRADYEKLKSEEHEKSHKLQELTFLYEKHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTSRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKNTVKRAHTAQIAKPIRPGHYPASSPTNPYGIRNSDSISYTNNLFQNYQNIYMRATSPSSETFFANPCSSTRGSSSSGSLLSYQKMNVDNGNATDINDNRSDLPCGCEAEDQSKLFPLHQETAAS

Target ORF information:

RefSeq Version	XM_012956286.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 5A (kif5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956286.2

ORF Insert Sequence:

ATGGCTGATA ACAGCATTAA CACTGAGTGC AGCATCAAGG TCCTGTGCAG GTTTCGGCCC 
CTGAACCAGT CGGAGGTGCA GAGAGGAGAC AAATTCCTGC CCATATTCCA AGGCGGGGAC 
ACTGTCATCG TTGGGGGTAA GCCTTATGCA TTTGATCGGG TGTTTCCTCC GAACACAACT 
CAGGAGCAAG TCTATCATGC CTGCGCTATG CAGATTGTTA AAGATGTGCT GGCAGGGTAT 
AATGGGACAA TCTTTGCTTA TGGGCAGACT GCATCTGGGA AGACCCACAC CATGGAGGGA 
AAACTTCATG ACCCACAATT GATGGGAATA ATTCCAAGGA TTGCCAGGGA TATCTTTAAC 
CATATTTATG CCATGGATGA GAACCTGGAA TTTCATATAA AGGTTTCCTA TTTTGAGATT 
TACTTGGATA AAATAAGAGA CCTGCTTGAT GTATCTAAAA CCAATCTCTC AGTTCATGAA 
GATAAAAACC GCGTTCCATT TGTCAAGGGT TGCACCGAGC GCTTTGTGTC CAGCCCAGAG 
GAGATCCTTG ATGTCATTGA TGAAGGGAAG TCGAACCGCC ATGTTGCAGT AACAAACATG 
AATGAGCACA GTTCCCGTAG CCACAGCATC TTCCTTATCA ATATTAAGCA AGAGAACATT 
GAGACGGAGC AGAAGCTCAG TGGGAAGCTG TACTTGGTTG ACCTGGCTGG AAGTGAAAAG 
GTCAGTAAGA CTGGAGCTGA AGGTTCAGTT TTGGATGAAG CCAAGAATAT CAACAAATCT 
CTGTCTGCTC TGGGAAATGT GATTTCTGCC CTGGCTGAAG GATCGAAAGG TTATGTGCCC 
TATAGAGACA GCAAGATGAC TCGAATTCTT CAGGACTCAT TGGGTGGAAA CTGCAGGACA 
ACAATGTTCA TCTGCTGCTC TCCATCCAGC TACAATGATG CAGAGACTAA GTCAACGCTG 
ATGTTTGGAC AGCGGGCTAA GACGATTAAG AACACTGCAT CTGTCAACCT GGAGCTGACT 
GCTGAGCAGT GGAAGAAGAA GTATGAGAAG GAAAAGGAGA AGAACAAGAC TCTGAAGGAG 
ACTATCCAGA AGCTGGAGGC TGAGCTCAGT CGCTGGAGGA ATGGGGAAAA TGTACCTGAA 
ACCGAGCAGC TGAGTGACGG GGAGCAGCTC ACCCAAGATA CTGCCAATGA AACGCCAGTT 
AATGATAATA ATTCATCCAT TGTCATCCGG ATCTCAGAGG AGGAAAGGAA GAAATATGAA 
GAGGAAATTC GGAAAATGTA CAAGCAGCTG GATGACAAGG ATGATGAAAT CAATCAGCAG 
AGCCAGCTTG TGGAAAAGCT GAAGCAACAG ATGTTGGACC AAGACGAGCT CCTGATTTCT 
ACCCGCGGTG ACAATGACAA GGTGCAGATG GAACTGAGCC GCCTGCAGTC AGAGAATGAC 
AGTGCCAAGG AAGAAGTCAA GGAAGTCCTC CAGGCACTGG AGGAACTTGC TGTAAACTAT 
GATCAGAAGT CTCAGGAGGT GGAAGATAAG ACCCTGCAGA ACAAGATTTT GGCAGATGAG 
CTGTCACAGA AAGTAGCCAA TCTGATGTCC CTGGAAAATG ACCTGCATCG CCTCCAGGAG 
TTTAGTTCTC ATCAACGCAA ACGAATCGCT GAAGTGCTGA ACGGCCTCAT GAAGGACCTT 
AGCGAGTTCA GCGTCATTGT AGGCAATGGT GAATTTAAGC TGCCTGTGGA GATCAGTGGT 
GCAATAGAAG AAGAGTTTAC AGTAGCCCGC TTGTACATCA GCAAGATCAA ATCGGAGGTG 
AAATCGGTGG TTAAACGCTG CCGCCAGTTA GAAAACCTTC AGGTGGAATG TCATCGCAAG 
ATGGAAGTTA CAGGAAGAGA ACTTTCCTCT TGTCAGCTGC TCATATCTCA GCATGAAGCA 
AAGATCAGGT CCCTGACAGA GTACATGCAC AGTGTTGAGT TGAAGAAGCG GCAGCTGGAG 
GAGTCCTATG ATGCACTGAG TGAGGAGTTG GCTAAGATGC AGGTGCAAGA AAATGTAACT 
GAAGTGGCAA TAAAGGATAA GGAGCAGGAT GCGATGGAAG CGTATGAAGT CAAGGTCCTG 
GAGATGCAGA TGGAGAGCCA CCGAGAGGCC CATCACAAAC AACTGGCTCG TCTCAGGGAT 
GAAATTAACG AGAAACAGAA GATCATTGAT GAGCTCAAAG ATCTGAATCA GAAGCTGCAG 
CTGGAGCTGG AAAAGCTTAG AGCTGATTAC GAGAAGCTGA AGAGCGAGGA ACATGAGAAG 
AGCCACAAGC TGCAGGAGCT GACATTTCTG TACGAGAAAC ACGAACAATC CAAACAGGAT 
CTTAAAGGGT TGGAGGAGAC AGTCGCCCGT GAACTCCAGA CCCTCCACAA CCTTCGGAAG 
CTTTTTGTTC AAGACGTCAC ATCTCGAGTC AAGAAAAGTG CTGAAATGGA ACCCGAGGAC 
AGTGGGGGGA TACACTCACA GAAACAGAAG ATTTCCTTTC TTGAGAACAA CCTGGAACAG 
CTTACAAAGG TTCACAAACA GTTGGTTCGT GACAATGCAG ATCTTCGTTG TGAGCTTCCT 
AAACTGGAAA AACGTCTTCG GGCTACAGCT GAGAGAGTTA AGGCCCTGGA GGGTGCACTG 
AAGGAGGCCA AGGAAGGAGC TATGAAAGAC AAGAGGCGGT ATCAACAGGA AGTAGATCGT 
ATTAAGGAGG CCGTACGCTA TAAGAACACA GTTAAACGTG CACACACAGC ACAGATTGCA 
AAGCCCATCC GGCCTGGGCA CTACCCTGCA TCCTCACCCA CCAACCCATA TGGCATCCGG 
AATTCTGACT CAATCAGTTA TACCAACAAC CTCTTTCAGA ACTACCAAAA TATATACATG 
CGGGCAACCA GCCCAAGCAG TGAAACATTC TTTGCCAATC CATGTTCCAG CACACGGGGC 
AGTTCTTCCA GTGGCAGCTT GCTTTCCTAT CAGAAGATGA ATGTGGATAA TGGAAATGCT 
ACAGATATCA ATGATAACAG AAGCGATTTG CCATGTGGCT GTGAAGCTGA GGATCAGTCC 
AAGTTGTTCC CTCTTCATCA GGAGACAGCA GCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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kif5a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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