tbck cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbck
Entrez Gene ID	100124764
Full Name	TBC1 domain containing kinase
Synonyms	hspc302,tbckl
General protein information	Preferred Names TBC1 domain containing kinase Names TBC domain-containing protein kinase-like protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001102743.1	NP_001096213.1	TBC domain-containing protein kinase-like protein
XM_012962097.2	XP_012817551.1	TBC domain-containing protein kinase-like protein isoform X1
XM_012962120.2	XP_012817574.1	TBC domain-containing protein kinase-like protein isoform X2
XM_012962156.2	XP_012817610.1	TBC domain-containing protein kinase-like protein isoform X2

Pathways from BioSystems

Homologies

	TBCK	XP_002804222.1
Mus musculus (house mouse)	Tbck	NP_001156927.1
Danio rerio (zebrafish)	tbck	NP_001074106.2
Homo sapiens (human)	TBCK	NP_001156908.1
Pan troglodytes (chimpanzee)	TBCK	XP_001170662.2
Canis lupus familiaris (dog)	TBCK	XP_545015.2
Gallus gallus (chicken)	TBCK	NP_001012595.1
Bos taurus (cattle)	TBCK	XP_002688132.1
Caenorhabditis elegans (roundworm)	tbck-1	NP_494866.2
Drosophila melanogaster (fruit fly)	CG4041	NP_572197.4
Xenopus tropicalis (tropical clawed frog)	tbck	NP_001096213.1
Rattus norvegicus (Norway rat)	Tbck	NP_001127985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000552	XP_310531.5

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tbck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa24588	NM_001102743.1 Latest version!	Xenopus tropicalis TBC1 domain containing kinase (tbck), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa30421	XM_012962097.2 Latest version!	Xenopus tropicalis TBC1 domain containing kinase (tbck), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa30422	XM_012962156.2 Latest version!	Xenopus tropicalis TBC1 domain containing kinase (tbck), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa30422	XM_012962120.2 Latest version!	Xenopus tropicalis TBC1 domain containing kinase (tbck), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbck ( NM_001102743.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001096213.1 Xenopus tropicalis TBC1 domain containing kinase (tbck), mRNA.

Price & Availability↑

CloneID	OXa24588
Clone ID Related Accession (Same CDS sequence)	NM_001102743.1
Accession Version	NM_001102743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC domain-containing protein kinase-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC136015.1. ##Evidence-Data-START## Transcript exon combination :: BC136015.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028292 [ECO:0000350] ##Evidence-Data-END##

ATGCCACCTT TAAAAGATGC AGAGCTTGGA GCCTTCACAT TCTTTGCATC TGCTCTCCCA 
CATGACGTTT GTGGAAGCAA TGGCCTTCCC CTCACACCGA ATTCAATAAA AATCTTGGGG 
CGATTCCAAA TCTTAAAAAC CATTGTGCAT CCCAGACTTT GCCAGTATGT TGACATATCT 
CGTGGTAAAC ATGAAAGGCT GGTGGTGGTA GCAGAACACT ATCCAAAAAG TTTTGAAGAC 
TTGCTACGGG AGGGAACTCC TGTGAGCTCT TCAAAGATCT TTCGTCTAGC TTATGAAGTT 
TTACAAGGCC TGGAGTATAT GAATCGCCAC CGAATGGTCC ATAGGGCACT TGCTCCTCAC 
AATATTCTTC TAGATGGAGA GGGACATGCC AAACTGGCCA AGTTTGGGCT TTATCATATC 
ACAGATCATG GGGCTGATGT TGATTTCCCA ATAGGATACC CTTCTTACCT GGCACCAGAA 
GTGATTGCTC AGGGGATTGT AAAGCCGACA GACCCCACAC AGTGCGAGAG GCCTCTGCCT 
TCAGGTCCAA AATCCGATGT CTGGTCTCTG GGTCTTATAT TGTTTGAACT GTGCATGGGT 
AGAAAATTAT TTCAGAGTCT GGATATCTCT GAGAAATTAA AACTAATACT TACATTAGGT 
TGTGTTGACG ACATTATAAC CGTACTTAGT GAAGAACATG GGTGTGAAGA TATTTTAAAG 
AGTGTCCCTG AAAATACCTT AGCCTTGTTG CGAAAGTGCC TCACGTTTCA TCCTACAAAA 
AGGCCTACCT CTGCGGAATT GCTTGCTGAC AGCGTGTTCA GTGAAGTGTC CCCTTTGTAC 
CTCCCTTTTC GCAAACCTGT TGGTCTCTTT TCTTCTGCTT TGCGATGTGC TAATCTAATA 
CTGCCTGAAG ATATTAGTCT CTTATGCAAA GATGAGGACA GTGACTTCCT TGCAGAAAGA 
TCTATTGAAG AAGTATATCA CTTGTGGTGC TTGGCAGGCG GAGACCTAGA GAAAGAGCTT 
ATAAGCAAGG GCATAATTCG ATCAAAGCCA CCTATCTGCA CTTTACCCAA CCTTCTCCTT 
GAAGATGGTG AAAGCTTTGG TCAAGGAAGG GACAGGAGCT CCCTGCTGGA TGACACCACT 
GTGACTCTTT CCTTGTGCCA ACTCAGAAAT AGATTAAAAG ATGTCCCTGG TGAAGCATAT 
TATCCATTAC TAGGTGAAGA CCAATCCAAC ATACCACATT CTGGCAGCAG CAATGAGTTG 
TCCACCTCAG CCACTCTTCC TCTGATTATT CGTGAGAGAG ACACAGAGTA TCAGCTAAAC 
AGAATTGTTT TATTTGACCG GTTGCTAAAT GCATATCCAT ACAAGAAAAA TATGATTTGG 
AAAGAGGCCA GAGTGGATAT TCCTCCTCTT TTGAGAGGAT TAACTTGGGC TGCTCTTCTT 
GGTGTAGAGG GGGCCATTCA AGAAAAATAT GATATGATAG ATAAGGACAC TCCAATACCT 
ACAGACCGTC AGATTGAAGT AGATATCCCA CGTTGCCATC AGTATGATGA GCTCCTTTCT 
TCTCCTGAAG GCCATGCAAA ATTTCGAAGG GTACTGAAAG CTTGGGTGGT TTCACATCCT 
AACCTGGTAT ATTGGCAAGG GTTGGATTCA CTGTGTGCAC CCTTCCTCTA CCTGAATTTT 
AACAATGAAG CGCTGGCATA TGCCTGTATG TCTGCCTTCA TACCCAAGTA CCTGTATAAT 
TTCTTTTTGA AGGACAATTC CCATGTAATA CAAGAGTACC TCAAAGTGTT CTCCCAAATG 
ATTGCCTTTC ATGACCCGGA ACTAAGCAAC CATCTCAATG AAATTGGCTT TATTCCAGAT 
CTCTACGCAA TTCCTTGGTT TCTTACAATG TTCACGCATG TTTTTCCTTT GCACAAGATA 
TTCCATCTCT GGGACACATT GCTGCTGGGG AATGCTTCAT TCCCATTTTG CATTGGAGTG 
GCCATTTTGC AACAGCTACG AGTCAGGCTT CTTGCGAATG GTTTTAATGA ATGCATTCTT 
CTGTTTTCCG ATTTGCCAGA AATTGACATT GAGCGCTGTG TCCGTGAGTC TGTTAATTTA 
TTTTGCTGGA CACCAAAGAG TGCAACATAC AGACAGCATG CACAGGTGCC AAAGCAGATC 
TCTGAAAGTA ATGGGGTGAG CAGGACATCA GCCAGCTTCA GTGTGGACTT CCAGGAATCC 
CCCAAGACTG ACTTGGGCCG AGAACCAATT AACATGAAAG AGCTGAAGTT AGAAGTTTCC 
CCACGAATCT CAGCAGAAGA TCTGATTGAC CTGTGTGAGC TGACCATGCC CAGTATGTCA 
CGGATACCAT CTAAAAAAAC CAGATCCATC AAGCCCAAGC TTTTGGTAGT GGATATTCGA 
CACAGTGAAG ACTTCAATCG TGGCCATATA CCTGGAAGCA TCAACATGCC GTCTGGCTCT 
GCATTCACAA CTGAAAGGGA TTTGGCTCCA TGTCCTGCAA GTACCACCCT GCAAAACTTC 
AAGGGTAAAG TGATTGTGAC TGTGGGGCAT GCAGCACAGG ATGCATCTGA GATAATAATA 
TTTACAGCAC GTGTTGTCAT GGACTTGGCT GCCTGTGGGA AAAAATTCAA TAACCATGGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096213.1
CDS	95..2737
Translation	MPPLKDAELGAFTFFASALPHDVCGSNGLPLTPNSIKILGRFQILKTIVHPRLCQYVDISRGKHERLVVVAEHYPKSFEDLLREGTPVSSSKIFRLAYEVLQGLEYMNRHRMVHRALAPHNILLDGEGHAKLAKFGLYHITDHGADVDFPIGYPSYLAPEVIAQGIVKPTDPTQCERPLPSGPKSDVWSLGLILFELCMGRKLFQSLDISEKLKLILTLGCVDDIITVLSEEHGCEDILKSVPENTLALLRKCLTFHPTKRPTSAELLADSVFSEVSPLYLPFRKPVGLFSSALRCANLILPEDISLLCKDEDSDFLAERSIEEVYHLWCLAGGDLEKELISKGIIRSKPPICTLPNLLLEDGESFGQGRDRSSLLDDTTVTLSLCQLRNRLKDVPGEAYYPLLGEDQSNIPHSGSSNELSTSATLPLIIRERDTEYQLNRIVLFDRLLNAYPYKKNMIWKEARVDIPPLLRGLTWAALLGVEGAIQEKYDMIDKDTPIPTDRQIEVDIPRCHQYDELLSSPEGHAKFRRVLKAWVVSHPNLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLKVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNASFPFCIGVAILQQLRVRLLANGFNECILLFSDLPEIDIERCVRESVNLFCWTPKSATYRQHAQVPKQISESNGVSRTSASFSVDFQESPKTDLGREPINMKELKLEVSPRISAEDLIDLCELTMPSMSRIPSKKTRSIKPKLLVVDIRHSEDFNRGHIPGSINMPSGSAFTTERDLAPCPASTTLQNFKGKVIVTVGHAAQDASEIIIFTARVVMDLAACGKKFNNHG

Target ORF information:

RefSeq Version	NM_001102743.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain containing kinase (tbck), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102743.1

ORF Insert Sequence:

ATGCCACCTT TAAAAGATGC AGAGCTTGGA GCCTTCACAT TCTTTGCATC TGCTCTCCCA 
CATGACGTTT GTGGAAGCAA TGGCCTTCCC CTCACACCGA ATTCAATAAA AATCTTGGGG 
CGATTCCAAA TCTTAAAAAC CATTGTGCAT CCCAGACTTT GCCAGTATGT TGACATATCT 
CGTGGTAAAC ATGAAAGGCT GGTGGTGGTA GCAGAACACT ATCCAAAAAG TTTTGAAGAC 
TTGCTACGGG AGGGAACTCC TGTGAGCTCT TCAAAGATCT TTCGTCTAGC TTATGAAGTT 
TTACAAGGCC TGGAGTATAT GAATCGCCAC CGAATGGTCC ATAGGGCACT TGCTCCTCAC 
AATATTCTTC TAGATGGAGA GGGACATGCC AAACTGGCCA AGTTTGGGCT TTATCATATC 
ACAGATCATG GGGCTGATGT TGATTTCCCA ATAGGATACC CTTCTTACCT GGCACCAGAA 
GTGATTGCTC AGGGGATTGT AAAGCCGACA GACCCCACAC AGTGCGAGAG GCCTCTGCCT 
TCAGGTCCAA AATCCGATGT CTGGTCTCTG GGTCTTATAT TGTTTGAACT GTGCATGGGT 
AGAAAATTAT TTCAGAGTCT GGATATCTCT GAGAAATTAA AACTAATACT TACATTAGGT 
TGTGTTGACG ACATTATAAC CGTACTTAGT GAAGAACATG GGTGTGAAGA TATTTTAAAG 
AGTGTCCCTG AAAATACCTT AGCCTTGTTG CGAAAGTGCC TCACGTTTCA TCCTACAAAA 
AGGCCTACCT CTGCGGAATT GCTTGCTGAC AGCGTGTTCA GTGAAGTGTC CCCTTTGTAC 
CTCCCTTTTC GCAAACCTGT TGGTCTCTTT TCTTCTGCTT TGCGATGTGC TAATCTAATA 
CTGCCTGAAG ATATTAGTCT CTTATGCAAA GATGAGGACA GTGACTTCCT TGCAGAAAGA 
TCTATTGAAG AAGTATATCA CTTGTGGTGC TTGGCAGGCG GAGACCTAGA GAAAGAGCTT 
ATAAGCAAGG GCATAATTCG ATCAAAGCCA CCTATCTGCA CTTTACCCAA CCTTCTCCTT 
GAAGATGGTG AAAGCTTTGG TCAAGGAAGG GACAGGAGCT CCCTGCTGGA TGACACCACT 
GTGACTCTTT CCTTGTGCCA ACTCAGAAAT AGATTAAAAG ATGTCCCTGG TGAAGCATAT 
TATCCATTAC TAGGTGAAGA CCAATCCAAC ATACCACATT CTGGCAGCAG CAATGAGTTG 
TCCACCTCAG CCACTCTTCC TCTGATTATT CGTGAGAGAG ACACAGAGTA TCAGCTAAAC 
AGAATTGTTT TATTTGACCG GTTGCTAAAT GCATATCCAT ACAAGAAAAA TATGATTTGG 
AAAGAGGCCA GAGTGGATAT TCCTCCTCTT TTGAGAGGAT TAACTTGGGC TGCTCTTCTT 
GGTGTAGAGG GGGCCATTCA AGAAAAATAT GATATGATAG ATAAGGACAC TCCAATACCT 
ACAGACCGTC AGATTGAAGT AGATATCCCA CGTTGCCATC AGTATGATGA GCTCCTTTCT 
TCTCCTGAAG GCCATGCAAA ATTTCGAAGG GTACTGAAAG CTTGGGTGGT TTCACATCCT 
AACCTGGTAT ATTGGCAAGG GTTGGATTCA CTGTGTGCAC CCTTCCTCTA CCTGAATTTT 
AACAATGAAG CGCTGGCATA TGCCTGTATG TCTGCCTTCA TACCCAAGTA CCTGTATAAT 
TTCTTTTTGA AGGACAATTC CCATGTAATA CAAGAGTACC TCAAAGTGTT CTCCCAAATG 
ATTGCCTTTC ATGACCCGGA ACTAAGCAAC CATCTCAATG AAATTGGCTT TATTCCAGAT 
CTCTACGCAA TTCCTTGGTT TCTTACAATG TTCACGCATG TTTTTCCTTT GCACAAGATA 
TTCCATCTCT GGGACACATT GCTGCTGGGG AATGCTTCAT TCCCATTTTG CATTGGAGTG 
GCCATTTTGC AACAGCTACG AGTCAGGCTT CTTGCGAATG GTTTTAATGA ATGCATTCTT 
CTGTTTTCCG ATTTGCCAGA AATTGACATT GAGCGCTGTG TCCGTGAGTC TGTTAATTTA 
TTTTGCTGGA CACCAAAGAG TGCAACATAC AGACAGCATG CACAGGTGCC AAAGCAGATC 
TCTGAAAGTA ATGGGGTGAG CAGGACATCA GCCAGCTTCA GTGTGGACTT CCAGGAATCC 
CCCAAGACTG ACTTGGGCCG AGAACCAATT AACATGAAAG AGCTGAAGTT AGAAGTTTCC 
CCACGAATCT CAGCAGAAGA TCTGATTGAC CTGTGTGAGC TGACCATGCC CAGTATGTCA 
CGGATACCAT CTAAAAAAAC CAGATCCATC AAGCCCAAGC TTTTGGTAGT GGATATTCGA 
CACAGTGAAG ACTTCAATCG TGGCCATATA CCTGGAAGCA TCAACATGCC GTCTGGCTCT 
GCATTCACAA CTGAAAGGGA TTTGGCTCCA TGTCCTGCAA GTACCACCCT GCAAAACTTC 
AAGGGTAAAG TGATTGTGAC TGTGGGGCAT GCAGCACAGG ATGCATCTGA GATAATAATA 
TTTACAGCAC GTGTTGTCAT GGACTTGGCT GCCTGTGGGA AAAAATTCAA TAACCATGGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbck ( XM_012962097.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817551.1 Xenopus tropicalis TBC1 domain containing kinase (tbck), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa30421
Clone ID Related Accession (Same CDS sequence)	XM_012962097.2
Accession Version	XM_012962097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC domain-containing protein kinase-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATGGA CAATGCCACC TTTAAAAGAT GCAGAGCTTG GAGCCTTCAC ATTCTTTGCA 
TCTGCTCTCC CACATGACGT TTGTGGAAGC AATGGCCTTC CCCTCACACC GAATTCAATA 
AAAATCTTGG GGCGATTCCA AATCTTAAAA ACCATTGTGC ATCCCAGACT TTGCCAGTAT 
GTTGACATAT CTCGTGGTAA ACATGAAAGG CTGGTGGTGG TAGCAGAACA CTATCCAAAA 
AGTTTTGAAG ACTTGCTACG GGAGGGAACT CCTGTGAGCT CTTCAAAGAT CTTTCGTCTA 
GCTTATGAAG TTTTACAAGG CCTGGAGTAT ATGAATCGCC ACCGAATGGT CCATAGGGCA 
CTTGCTCCTC ACAATATTCT TCTAGATGGA GAGGGACATG CCAAACTGGC CAAGTTTGGG 
CTTTATCATA TCACAGATCA TGGGGCTGAT GTTGATTTCC CAATAGGATA CCCTTCTTAC 
CTGGCACCAG AAGTGATTGC TCAGGGGATT GTAAAGCCGA CAGACCCCAC ACAGTGCGAG 
AGGCCTCTGC CTTCAGGTCC AAAATCCGAT GTCTGGTCTC TGGGTCTTAT ATTGTTTGAA 
CTGTGCATGG GTAGAAAATT ATTTCAGAGT CTGGATATCT CTGAGAAATT AAAACTAATA 
CTTACATTAG GTTGTGTTGA CGACATTATA ACCGTACTTA GTGAAGAACA TGGGTGTGAA 
GATATTTTAA AGAGTGTCCC TGAAAATACC TTAGCCTTGT TGCGAAAGTG CCTCACGTTT 
CATCCTACAA AAAGGCCTAC CTCTGCGGAA TTGCTTGCTG ACAGCGTGTT CAGTGAAGTG 
TCCCCTTTGT ACCTCCCTTT TCGCAAACCT GTTGGTCTCT TTTCTTCTGC TTTGCGATGT 
GCTAATCTAA TACTGCCTGA AGATATTAGT CTCTTATGCA AAGATGAGGA CAGTGACTTC 
CTTGCAGAAA GATCTATTGA AGAAGTATAT CACTTGTGGT GCTTGGCAGG CGGAGACCTA 
GAGAAAGAGC TTATAAGCAA GGGCATAATT CGATCAAAGC CACCTATCTG CACTTTACCC 
AACCTTCTCC TTGAAGATGG TGAAAGCTTT GGTCAAGGAA GGGACAGGAG CTCCCTGCTG 
GATGACACCA CTGTGACTCT TTCCTTGTGC CAACTCAGAA ATAGATTAAA AGATGTCCCT 
GGTGAAGCAT ATTATCCATT ACTAGGTGAA GACCAATCCA ACATACCACA TTCTGGCAGC 
AGCAATGAGT TGTCCACCTC AGCCACTCTT CCTCTGATTA TTCGTGAGAG AGACACAGAG 
TATCAGCTAA ACAGAATTGT TTTATTTGAC CGGTTGCTAA ATGCATATCC ATACAAGAAA 
AATATGATTT GGAAAGAGGC CAGAGTGGAT ATTCCTCCTC TTTTGAGAGG ATTAACTTGG 
GCTGCTCTTC TTGGTGTAGA GGGGGCCATT CAAGAAAAAT ATGATATGAT AGATAAGGAC 
ACTCCAATAC CTACAGACCG TCAGATTGAA GTAGATATCC CACGTTGCCA TCAGTATGAT 
GAGCTCCTTT CTTCTCCTGA AGGCCATGCA AAATTTCGAA GGGTACTGAA AGCTTGGGTG 
GTTTCACATC CTAACCTGGT ATATTGGCAA GGGTTGGATT CACTGTGTGC ACCCTTCCTC 
TACCTGAATT TTAACAATGA AGCGCTGGCA TATGCCTGTA TGTCTGCCTT CATACCCAAG 
TACCTGTATA ATTTCTTTTT GAAGGACAAT TCCCATGTAA TACAAGAGTA CCTCAAAGTG 
TTCTCCCAAA TGATTGCCTT TCATGACCCG GAACTAAGCA ACCATCTCAA TGAAATTGGC 
TTTATTCCAG ATCTCTACGC AATTCCTTGG TTTCTTACAA TGTTCACGCA TGTTTTTCCT 
TTGCACAAGA TATTCCATCT CTGGGACACA TTGCTGCTGG GGAATGCTTC CTTCCCATTT 
TGCATTGGAG TGGCCATTTT GCAACAGCTA CGAGTCAGGC TTCTTGCGAA TGGTTTTAAT 
GAATGCATTC TTCTGTTTTC CGATTTGCCA GAAATTGACA TTGAGCGCTG TGTCCGTGAG 
TCTGTTAATT TATTTTGCTG GACACCAAAG AGTGCAACAT ACAGACAGCA TGCACAGGTG 
CCAAAGCAGA TCTCTGAAAG TAATGGGGTG AGCAGGACAT CAGCCAGCTT CAGTGTGGAC 
TTCCAGGAAT CCCCCAAGAC TGACTTGGGC CGAGAACCAA TTAACATGAA AGAGCTGAAG 
TTAGAAGTTT CCCCACGAAT CTCAGCAGAA GATCTGATTG ACCTGTGTGA GCTGACCATG 
CCCAGTATGT CACGGATACC ATCTAAAAAA ACCAGATCCA TCAAGCCCAA GCTTTTGGTA 
GTGGATATTC GACACAGTGA AGATTTCAAT CGTGGCCATA TACCTGGAAG CATCAACATG 
CCGTCTGGCT CTGCATTCAC AACTGAAAGG GATTTGGCTC CATGTCCTGC AAGTACCACC 
CTGCAAAACT TCAAGGGTAA AGTGATTGTG ACTGTGGGGC ATGCAGCACA GGATGCATCT 
GAGTTTGCAG TTAACTTGGT AAAGCTGGGA TACCCAAGAG TTTGTATTCT GGATGGCGGC 
ATTAACAAGA TGAAGCTTAC TGGCTTGCTG ACTGTTCCCT CCCCTCAGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817551.1
CDS	153..2846
Translation	MVWTMPPLKDAELGAFTFFASALPHDVCGSNGLPLTPNSIKILGRFQILKTIVHPRLCQYVDISRGKHERLVVVAEHYPKSFEDLLREGTPVSSSKIFRLAYEVLQGLEYMNRHRMVHRALAPHNILLDGEGHAKLAKFGLYHITDHGADVDFPIGYPSYLAPEVIAQGIVKPTDPTQCERPLPSGPKSDVWSLGLILFELCMGRKLFQSLDISEKLKLILTLGCVDDIITVLSEEHGCEDILKSVPENTLALLRKCLTFHPTKRPTSAELLADSVFSEVSPLYLPFRKPVGLFSSALRCANLILPEDISLLCKDEDSDFLAERSIEEVYHLWCLAGGDLEKELISKGIIRSKPPICTLPNLLLEDGESFGQGRDRSSLLDDTTVTLSLCQLRNRLKDVPGEAYYPLLGEDQSNIPHSGSSNELSTSATLPLIIRERDTEYQLNRIVLFDRLLNAYPYKKNMIWKEARVDIPPLLRGLTWAALLGVEGAIQEKYDMIDKDTPIPTDRQIEVDIPRCHQYDELLSSPEGHAKFRRVLKAWVVSHPNLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLKVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNASFPFCIGVAILQQLRVRLLANGFNECILLFSDLPEIDIERCVRESVNLFCWTPKSATYRQHAQVPKQISESNGVSRTSASFSVDFQESPKTDLGREPINMKELKLEVSPRISAEDLIDLCELTMPSMSRIPSKKTRSIKPKLLVVDIRHSEDFNRGHIPGSINMPSGSAFTTERDLAPCPASTTLQNFKGKVIVTVGHAAQDASEFAVNLVKLGYPRVCILDGGINKMKLTGLLTVPSPQI

Target ORF information:

RefSeq Version	XM_012962097.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain containing kinase (tbck), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962097.2

ORF Insert Sequence:

ATGGTATGGA CAATGCCACC TTTAAAAGAT GCAGAGCTTG GAGCCTTCAC ATTCTTTGCA 
TCTGCTCTCC CACATGACGT TTGTGGAAGC AATGGCCTTC CCCTCACACC GAATTCAATA 
AAAATCTTGG GGCGATTCCA AATCTTAAAA ACCATTGTGC ATCCCAGACT TTGCCAGTAT 
GTTGACATAT CTCGTGGTAA ACATGAAAGG CTGGTGGTGG TAGCAGAACA CTATCCAAAA 
AGTTTTGAAG ACTTGCTACG GGAGGGAACT CCTGTGAGCT CTTCAAAGAT CTTTCGTCTA 
GCTTATGAAG TTTTACAAGG CCTGGAGTAT ATGAATCGCC ACCGAATGGT CCATAGGGCA 
CTTGCTCCTC ACAATATTCT TCTAGATGGA GAGGGACATG CCAAACTGGC CAAGTTTGGG 
CTTTATCATA TCACAGATCA TGGGGCTGAT GTTGATTTCC CAATAGGATA CCCTTCTTAC 
CTGGCACCAG AAGTGATTGC TCAGGGGATT GTAAAGCCGA CAGACCCCAC ACAGTGCGAG 
AGGCCTCTGC CTTCAGGTCC AAAATCCGAT GTCTGGTCTC TGGGTCTTAT ATTGTTTGAA 
CTGTGCATGG GTAGAAAATT ATTTCAGAGT CTGGATATCT CTGAGAAATT AAAACTAATA 
CTTACATTAG GTTGTGTTGA CGACATTATA ACCGTACTTA GTGAAGAACA TGGGTGTGAA 
GATATTTTAA AGAGTGTCCC TGAAAATACC TTAGCCTTGT TGCGAAAGTG CCTCACGTTT 
CATCCTACAA AAAGGCCTAC CTCTGCGGAA TTGCTTGCTG ACAGCGTGTT CAGTGAAGTG 
TCCCCTTTGT ACCTCCCTTT TCGCAAACCT GTTGGTCTCT TTTCTTCTGC TTTGCGATGT 
GCTAATCTAA TACTGCCTGA AGATATTAGT CTCTTATGCA AAGATGAGGA CAGTGACTTC 
CTTGCAGAAA GATCTATTGA AGAAGTATAT CACTTGTGGT GCTTGGCAGG CGGAGACCTA 
GAGAAAGAGC TTATAAGCAA GGGCATAATT CGATCAAAGC CACCTATCTG CACTTTACCC 
AACCTTCTCC TTGAAGATGG TGAAAGCTTT GGTCAAGGAA GGGACAGGAG CTCCCTGCTG 
GATGACACCA CTGTGACTCT TTCCTTGTGC CAACTCAGAA ATAGATTAAA AGATGTCCCT 
GGTGAAGCAT ATTATCCATT ACTAGGTGAA GACCAATCCA ACATACCACA TTCTGGCAGC 
AGCAATGAGT TGTCCACCTC AGCCACTCTT CCTCTGATTA TTCGTGAGAG AGACACAGAG 
TATCAGCTAA ACAGAATTGT TTTATTTGAC CGGTTGCTAA ATGCATATCC ATACAAGAAA 
AATATGATTT GGAAAGAGGC CAGAGTGGAT ATTCCTCCTC TTTTGAGAGG ATTAACTTGG 
GCTGCTCTTC TTGGTGTAGA GGGGGCCATT CAAGAAAAAT ATGATATGAT AGATAAGGAC 
ACTCCAATAC CTACAGACCG TCAGATTGAA GTAGATATCC CACGTTGCCA TCAGTATGAT 
GAGCTCCTTT CTTCTCCTGA AGGCCATGCA AAATTTCGAA GGGTACTGAA AGCTTGGGTG 
GTTTCACATC CTAACCTGGT ATATTGGCAA GGGTTGGATT CACTGTGTGC ACCCTTCCTC 
TACCTGAATT TTAACAATGA AGCGCTGGCA TATGCCTGTA TGTCTGCCTT CATACCCAAG 
TACCTGTATA ATTTCTTTTT GAAGGACAAT TCCCATGTAA TACAAGAGTA CCTCAAAGTG 
TTCTCCCAAA TGATTGCCTT TCATGACCCG GAACTAAGCA ACCATCTCAA TGAAATTGGC 
TTTATTCCAG ATCTCTACGC AATTCCTTGG TTTCTTACAA TGTTCACGCA TGTTTTTCCT 
TTGCACAAGA TATTCCATCT CTGGGACACA TTGCTGCTGG GGAATGCTTC CTTCCCATTT 
TGCATTGGAG TGGCCATTTT GCAACAGCTA CGAGTCAGGC TTCTTGCGAA TGGTTTTAAT 
GAATGCATTC TTCTGTTTTC CGATTTGCCA GAAATTGACA TTGAGCGCTG TGTCCGTGAG 
TCTGTTAATT TATTTTGCTG GACACCAAAG AGTGCAACAT ACAGACAGCA TGCACAGGTG 
CCAAAGCAGA TCTCTGAAAG TAATGGGGTG AGCAGGACAT CAGCCAGCTT CAGTGTGGAC 
TTCCAGGAAT CCCCCAAGAC TGACTTGGGC CGAGAACCAA TTAACATGAA AGAGCTGAAG 
TTAGAAGTTT CCCCACGAAT CTCAGCAGAA GATCTGATTG ACCTGTGTGA GCTGACCATG 
CCCAGTATGT CACGGATACC ATCTAAAAAA ACCAGATCCA TCAAGCCCAA GCTTTTGGTA 
GTGGATATTC GACACAGTGA AGATTTCAAT CGTGGCCATA TACCTGGAAG CATCAACATG 
CCGTCTGGCT CTGCATTCAC AACTGAAAGG GATTTGGCTC CATGTCCTGC AAGTACCACC 
CTGCAAAACT TCAAGGGTAA AGTGATTGTG ACTGTGGGGC ATGCAGCACA GGATGCATCT 
GAGTTTGCAG TTAACTTGGT AAAGCTGGGA TACCCAAGAG TTTGTATTCT GGATGGCGGC 
ATTAACAAGA TGAAGCTTAC TGGCTTGCTG ACTGTTCCCT CCCCTCAGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbck ( XM_012962120.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817574.1 Xenopus tropicalis TBC1 domain containing kinase (tbck), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa30422
Clone ID Related Accession (Same CDS sequence)	XM_012962120.2 , XM_012962156.2
Accession Version	XM_012962120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC domain-containing protein kinase-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCGCC ACCGAATGGT CCATAGGGCA CTTGCTCCTC ACAATATTCT TCTAGATGGA 
GAGGGACATG CCAAACTGGC CAAGTTTGGG CTTTATCATA TCACAGATCA TGGGGCTGAT 
GTTGATTTCC CAATAGGATA CCCTTCTTAC CTGGCACCAG AAGTGATTGC TCAGGGGATT 
GTAAAGCCGA CAGACCCCAC ACAGTGCGAG AGGCCTCTGC CTTCAGGTCC AAAATCCGAT 
GTCTGGTCTC TGGGTCTTAT ATTGTTTGAA CTGTGCATGG GTAGAAAATT ATTTCAGAGT 
CTGGATATCT CTGAGAAATT AAAACTAATA CTTACATTAG GTTGTGTTGA CGACATTATA 
ACCGTACTTA GTGAAGAACA TGGGTGTGAA GATATTTTAA AGAGTGTCCC TGAAAATACC 
TTAGCCTTGT TGCGAAAGTG CCTCACGTTT CATCCTACAA AAAGGCCTAC CTCTGCGGAA 
TTGCTTGCTG ACAGCGTGTT CAGTGAAGTG TCCCCTTTGT ACCTCCCTTT TCGCAAACCT 
GTTGGTCTCT TTTCTTCTGC TTTGCGATGT GCTAATCTAA TACTGCCTGA AGATATTAGT 
CTCTTATGCA AAGATGAGGA CAGTGACTTC CTTGCAGAAA GATCTATTGA AGAAGTATAT 
CACTTGTGGT GCTTGGCAGG CGGAGACCTA GAGAAAGAGC TTATAAGCAA GGGCATAATT 
CGATCAAAGC CACCTATCTG CACTTTACCC AACCTTCTCC TTGAAGATGG TGAAAGCTTT 
GGTCAAGGAA GGGACAGGAG CTCCCTGCTG GATGACACCA CTGTGACTCT TTCCTTGTGC 
CAACTCAGAA ATAGATTAAA AGATGTCCCT GGTGAAGCAT ATTATCCATT ACTAGGTGAA 
GACCAATCCA ACATACCACA TTCTGGCAGC AGCAATGAGT TGTCCACCTC AGCCACTCTT 
CCTCTGATTA TTCGTGAGAG AGACACAGAG TATCAGCTAA ACAGAATTGT TTTATTTGAC 
CGGTTGCTAA ATGCATATCC ATACAAGAAA AATATGATTT GGAAAGAGGC CAGAGTGGAT 
ATTCCTCCTC TTTTGAGAGG ATTAACTTGG GCTGCTCTTC TTGGTGTAGA GGGGGCCATT 
CAAGAAAAAT ATGATATGAT AGATAAGGAC ACTCCAATAC CTACAGACCG TCAGATTGAA 
GTAGATATCC CACGTTGCCA TCAGTATGAT GAGCTCCTTT CTTCTCCTGA AGGCCATGCA 
AAATTTCGAA GGGTACTGAA AGCTTGGGTG GTTTCACATC CTAACCTGGT ATATTGGCAA 
GGGTTGGATT CACTGTGTGC ACCCTTCCTC TACCTGAATT TTAACAATGA AGCGCTGGCA 
TATGCCTGTA TGTCTGCCTT CATACCCAAG TACCTGTATA ATTTCTTTTT GAAGGACAAT 
TCCCATGTAA TACAAGAGTA CCTCAAAGTG TTCTCCCAAA TGATTGCCTT TCATGACCCG 
GAACTAAGCA ACCATCTCAA TGAAATTGGC TTTATTCCAG ATCTCTACGC AATTCCTTGG 
TTTCTTACAA TGTTCACGCA TGTTTTTCCT TTGCACAAGA TATTCCATCT CTGGGACACA 
TTGCTGCTGG GGAATGCTTC CTTCCCATTT TGCATTGGAG TGGCCATTTT GCAACAGCTA 
CGAGTCAGGC TTCTTGCGAA TGGTTTTAAT GAATGCATTC TTCTGTTTTC CGATTTGCCA 
GAAATTGACA TTGAGCGCTG TGTCCGTGAG TCTGTTAATT TATTTTGCTG GACACCAAAG 
AGTGCAACAT ACAGACAGCA TGCACAGGTG CCAAAGCAGA TCTCTGAAAG TAATGGGGTG 
AGCAGGACAT CAGCCAGCTT CAGTGTGGAC TTCCAGGAAT CCCCCAAGAC TGACTTGGGC 
CGAGAACCAA TTAACATGAA AGAGCTGAAG TTAGAAGTTT CCCCACGAAT CTCAGCAGAA 
GATCTGATTG ACCTGTGTGA GCTGACCATG CCCAGTATGT CACGGATACC ATCTAAAAAA 
ACCAGATCCA TCAAGCCCAA GCTTTTGGTA GTGGATATTC GACACAGTGA AGATTTCAAT 
CGTGGCCATA TACCTGGAAG CATCAACATG CCGTCTGGCT CTGCATTCAC AACTGAAAGG 
GATTTGGCTC CATGTCCTGC AAGTACCACC CTGCAAAACT TCAAGGGTAA AGTGATTGTG 
ACTGTGGGGC ATGCAGCACA GGATGCATCT GAGTTTGCAG TTAACTTGGT AAAGCTGGGA 
TACCCAAGAG TTTGTATTCT GGATGGCGGC ATTAACAAGA TGAAGCTTAC TGGCTTGCTG 
ACTGTTCCCT CCCCTCAGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817574.1
CDS	270..2633
Translation	MNRHRMVHRALAPHNILLDGEGHAKLAKFGLYHITDHGADVDFPIGYPSYLAPEVIAQGIVKPTDPTQCERPLPSGPKSDVWSLGLILFELCMGRKLFQSLDISEKLKLILTLGCVDDIITVLSEEHGCEDILKSVPENTLALLRKCLTFHPTKRPTSAELLADSVFSEVSPLYLPFRKPVGLFSSALRCANLILPEDISLLCKDEDSDFLAERSIEEVYHLWCLAGGDLEKELISKGIIRSKPPICTLPNLLLEDGESFGQGRDRSSLLDDTTVTLSLCQLRNRLKDVPGEAYYPLLGEDQSNIPHSGSSNELSTSATLPLIIRERDTEYQLNRIVLFDRLLNAYPYKKNMIWKEARVDIPPLLRGLTWAALLGVEGAIQEKYDMIDKDTPIPTDRQIEVDIPRCHQYDELLSSPEGHAKFRRVLKAWVVSHPNLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLKVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNASFPFCIGVAILQQLRVRLLANGFNECILLFSDLPEIDIERCVRESVNLFCWTPKSATYRQHAQVPKQISESNGVSRTSASFSVDFQESPKTDLGREPINMKELKLEVSPRISAEDLIDLCELTMPSMSRIPSKKTRSIKPKLLVVDIRHSEDFNRGHIPGSINMPSGSAFTTERDLAPCPASTTLQNFKGKVIVTVGHAAQDASEFAVNLVKLGYPRVCILDGGINKMKLTGLLTVPSPQI

Target ORF information:

RefSeq Version	XM_012962120.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain containing kinase (tbck), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962120.2

ORF Insert Sequence:

ATGAATCGCC ACCGAATGGT CCATAGGGCA CTTGCTCCTC ACAATATTCT TCTAGATGGA 
GAGGGACATG CCAAACTGGC CAAGTTTGGG CTTTATCATA TCACAGATCA TGGGGCTGAT 
GTTGATTTCC CAATAGGATA CCCTTCTTAC CTGGCACCAG AAGTGATTGC TCAGGGGATT 
GTAAAGCCGA CAGACCCCAC ACAGTGCGAG AGGCCTCTGC CTTCAGGTCC AAAATCCGAT 
GTCTGGTCTC TGGGTCTTAT ATTGTTTGAA CTGTGCATGG GTAGAAAATT ATTTCAGAGT 
CTGGATATCT CTGAGAAATT AAAACTAATA CTTACATTAG GTTGTGTTGA CGACATTATA 
ACCGTACTTA GTGAAGAACA TGGGTGTGAA GATATTTTAA AGAGTGTCCC TGAAAATACC 
TTAGCCTTGT TGCGAAAGTG CCTCACGTTT CATCCTACAA AAAGGCCTAC CTCTGCGGAA 
TTGCTTGCTG ACAGCGTGTT CAGTGAAGTG TCCCCTTTGT ACCTCCCTTT TCGCAAACCT 
GTTGGTCTCT TTTCTTCTGC TTTGCGATGT GCTAATCTAA TACTGCCTGA AGATATTAGT 
CTCTTATGCA AAGATGAGGA CAGTGACTTC CTTGCAGAAA GATCTATTGA AGAAGTATAT 
CACTTGTGGT GCTTGGCAGG CGGAGACCTA GAGAAAGAGC TTATAAGCAA GGGCATAATT 
CGATCAAAGC CACCTATCTG CACTTTACCC AACCTTCTCC TTGAAGATGG TGAAAGCTTT 
GGTCAAGGAA GGGACAGGAG CTCCCTGCTG GATGACACCA CTGTGACTCT TTCCTTGTGC 
CAACTCAGAA ATAGATTAAA AGATGTCCCT GGTGAAGCAT ATTATCCATT ACTAGGTGAA 
GACCAATCCA ACATACCACA TTCTGGCAGC AGCAATGAGT TGTCCACCTC AGCCACTCTT 
CCTCTGATTA TTCGTGAGAG AGACACAGAG TATCAGCTAA ACAGAATTGT TTTATTTGAC 
CGGTTGCTAA ATGCATATCC ATACAAGAAA AATATGATTT GGAAAGAGGC CAGAGTGGAT 
ATTCCTCCTC TTTTGAGAGG ATTAACTTGG GCTGCTCTTC TTGGTGTAGA GGGGGCCATT 
CAAGAAAAAT ATGATATGAT AGATAAGGAC ACTCCAATAC CTACAGACCG TCAGATTGAA 
GTAGATATCC CACGTTGCCA TCAGTATGAT GAGCTCCTTT CTTCTCCTGA AGGCCATGCA 
AAATTTCGAA GGGTACTGAA AGCTTGGGTG GTTTCACATC CTAACCTGGT ATATTGGCAA 
GGGTTGGATT CACTGTGTGC ACCCTTCCTC TACCTGAATT TTAACAATGA AGCGCTGGCA 
TATGCCTGTA TGTCTGCCTT CATACCCAAG TACCTGTATA ATTTCTTTTT GAAGGACAAT 
TCCCATGTAA TACAAGAGTA CCTCAAAGTG TTCTCCCAAA TGATTGCCTT TCATGACCCG 
GAACTAAGCA ACCATCTCAA TGAAATTGGC TTTATTCCAG ATCTCTACGC AATTCCTTGG 
TTTCTTACAA TGTTCACGCA TGTTTTTCCT TTGCACAAGA TATTCCATCT CTGGGACACA 
TTGCTGCTGG GGAATGCTTC CTTCCCATTT TGCATTGGAG TGGCCATTTT GCAACAGCTA 
CGAGTCAGGC TTCTTGCGAA TGGTTTTAAT GAATGCATTC TTCTGTTTTC CGATTTGCCA 
GAAATTGACA TTGAGCGCTG TGTCCGTGAG TCTGTTAATT TATTTTGCTG GACACCAAAG 
AGTGCAACAT ACAGACAGCA TGCACAGGTG CCAAAGCAGA TCTCTGAAAG TAATGGGGTG 
AGCAGGACAT CAGCCAGCTT CAGTGTGGAC TTCCAGGAAT CCCCCAAGAC TGACTTGGGC 
CGAGAACCAA TTAACATGAA AGAGCTGAAG TTAGAAGTTT CCCCACGAAT CTCAGCAGAA 
GATCTGATTG ACCTGTGTGA GCTGACCATG CCCAGTATGT CACGGATACC ATCTAAAAAA 
ACCAGATCCA TCAAGCCCAA GCTTTTGGTA GTGGATATTC GACACAGTGA AGATTTCAAT 
CGTGGCCATA TACCTGGAAG CATCAACATG CCGTCTGGCT CTGCATTCAC AACTGAAAGG 
GATTTGGCTC CATGTCCTGC AAGTACCACC CTGCAAAACT TCAAGGGTAA AGTGATTGTG 
ACTGTGGGGC ATGCAGCACA GGATGCATCT GAGTTTGCAG TTAACTTGGT AAAGCTGGGA 
TACCCAAGAG TTTGTATTCT GGATGGCGGC ATTAACAAGA TGAAGCTTAC TGGCTTGCTG 
ACTGTTCCCT CCCCTCAGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbck ( XM_012962156.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817610.1 Xenopus tropicalis TBC1 domain containing kinase (tbck), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa30422
Clone ID Related Accession (Same CDS sequence)	XM_012962120.2 , XM_012962156.2
Accession Version	XM_012962156.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC domain-containing protein kinase-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCGCC ACCGAATGGT CCATAGGGCA CTTGCTCCTC ACAATATTCT TCTAGATGGA 
GAGGGACATG CCAAACTGGC CAAGTTTGGG CTTTATCATA TCACAGATCA TGGGGCTGAT 
GTTGATTTCC CAATAGGATA CCCTTCTTAC CTGGCACCAG AAGTGATTGC TCAGGGGATT 
GTAAAGCCGA CAGACCCCAC ACAGTGCGAG AGGCCTCTGC CTTCAGGTCC AAAATCCGAT 
GTCTGGTCTC TGGGTCTTAT ATTGTTTGAA CTGTGCATGG GTAGAAAATT ATTTCAGAGT 
CTGGATATCT CTGAGAAATT AAAACTAATA CTTACATTAG GTTGTGTTGA CGACATTATA 
ACCGTACTTA GTGAAGAACA TGGGTGTGAA GATATTTTAA AGAGTGTCCC TGAAAATACC 
TTAGCCTTGT TGCGAAAGTG CCTCACGTTT CATCCTACAA AAAGGCCTAC CTCTGCGGAA 
TTGCTTGCTG ACAGCGTGTT CAGTGAAGTG TCCCCTTTGT ACCTCCCTTT TCGCAAACCT 
GTTGGTCTCT TTTCTTCTGC TTTGCGATGT GCTAATCTAA TACTGCCTGA AGATATTAGT 
CTCTTATGCA AAGATGAGGA CAGTGACTTC CTTGCAGAAA GATCTATTGA AGAAGTATAT 
CACTTGTGGT GCTTGGCAGG CGGAGACCTA GAGAAAGAGC TTATAAGCAA GGGCATAATT 
CGATCAAAGC CACCTATCTG CACTTTACCC AACCTTCTCC TTGAAGATGG TGAAAGCTTT 
GGTCAAGGAA GGGACAGGAG CTCCCTGCTG GATGACACCA CTGTGACTCT TTCCTTGTGC 
CAACTCAGAA ATAGATTAAA AGATGTCCCT GGTGAAGCAT ATTATCCATT ACTAGGTGAA 
GACCAATCCA ACATACCACA TTCTGGCAGC AGCAATGAGT TGTCCACCTC AGCCACTCTT 
CCTCTGATTA TTCGTGAGAG AGACACAGAG TATCAGCTAA ACAGAATTGT TTTATTTGAC 
CGGTTGCTAA ATGCATATCC ATACAAGAAA AATATGATTT GGAAAGAGGC CAGAGTGGAT 
ATTCCTCCTC TTTTGAGAGG ATTAACTTGG GCTGCTCTTC TTGGTGTAGA GGGGGCCATT 
CAAGAAAAAT ATGATATGAT AGATAAGGAC ACTCCAATAC CTACAGACCG TCAGATTGAA 
GTAGATATCC CACGTTGCCA TCAGTATGAT GAGCTCCTTT CTTCTCCTGA AGGCCATGCA 
AAATTTCGAA GGGTACTGAA AGCTTGGGTG GTTTCACATC CTAACCTGGT ATATTGGCAA 
GGGTTGGATT CACTGTGTGC ACCCTTCCTC TACCTGAATT TTAACAATGA AGCGCTGGCA 
TATGCCTGTA TGTCTGCCTT CATACCCAAG TACCTGTATA ATTTCTTTTT GAAGGACAAT 
TCCCATGTAA TACAAGAGTA CCTCAAAGTG TTCTCCCAAA TGATTGCCTT TCATGACCCG 
GAACTAAGCA ACCATCTCAA TGAAATTGGC TTTATTCCAG ATCTCTACGC AATTCCTTGG 
TTTCTTACAA TGTTCACGCA TGTTTTTCCT TTGCACAAGA TATTCCATCT CTGGGACACA 
TTGCTGCTGG GGAATGCTTC CTTCCCATTT TGCATTGGAG TGGCCATTTT GCAACAGCTA 
CGAGTCAGGC TTCTTGCGAA TGGTTTTAAT GAATGCATTC TTCTGTTTTC CGATTTGCCA 
GAAATTGACA TTGAGCGCTG TGTCCGTGAG TCTGTTAATT TATTTTGCTG GACACCAAAG 
AGTGCAACAT ACAGACAGCA TGCACAGGTG CCAAAGCAGA TCTCTGAAAG TAATGGGGTG 
AGCAGGACAT CAGCCAGCTT CAGTGTGGAC TTCCAGGAAT CCCCCAAGAC TGACTTGGGC 
CGAGAACCAA TTAACATGAA AGAGCTGAAG TTAGAAGTTT CCCCACGAAT CTCAGCAGAA 
GATCTGATTG ACCTGTGTGA GCTGACCATG CCCAGTATGT CACGGATACC ATCTAAAAAA 
ACCAGATCCA TCAAGCCCAA GCTTTTGGTA GTGGATATTC GACACAGTGA AGATTTCAAT 
CGTGGCCATA TACCTGGAAG CATCAACATG CCGTCTGGCT CTGCATTCAC AACTGAAAGG 
GATTTGGCTC CATGTCCTGC AAGTACCACC CTGCAAAACT TCAAGGGTAA AGTGATTGTG 
ACTGTGGGGC ATGCAGCACA GGATGCATCT GAGTTTGCAG TTAACTTGGT AAAGCTGGGA 
TACCCAAGAG TTTGTATTCT GGATGGCGGC ATTAACAAGA TGAAGCTTAC TGGCTTGCTG 
ACTGTTCCCT CCCCTCAGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817610.1
CDS	95..2458
Translation	MNRHRMVHRALAPHNILLDGEGHAKLAKFGLYHITDHGADVDFPIGYPSYLAPEVIAQGIVKPTDPTQCERPLPSGPKSDVWSLGLILFELCMGRKLFQSLDISEKLKLILTLGCVDDIITVLSEEHGCEDILKSVPENTLALLRKCLTFHPTKRPTSAELLADSVFSEVSPLYLPFRKPVGLFSSALRCANLILPEDISLLCKDEDSDFLAERSIEEVYHLWCLAGGDLEKELISKGIIRSKPPICTLPNLLLEDGESFGQGRDRSSLLDDTTVTLSLCQLRNRLKDVPGEAYYPLLGEDQSNIPHSGSSNELSTSATLPLIIRERDTEYQLNRIVLFDRLLNAYPYKKNMIWKEARVDIPPLLRGLTWAALLGVEGAIQEKYDMIDKDTPIPTDRQIEVDIPRCHQYDELLSSPEGHAKFRRVLKAWVVSHPNLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLKVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNASFPFCIGVAILQQLRVRLLANGFNECILLFSDLPEIDIERCVRESVNLFCWTPKSATYRQHAQVPKQISESNGVSRTSASFSVDFQESPKTDLGREPINMKELKLEVSPRISAEDLIDLCELTMPSMSRIPSKKTRSIKPKLLVVDIRHSEDFNRGHIPGSINMPSGSAFTTERDLAPCPASTTLQNFKGKVIVTVGHAAQDASEFAVNLVKLGYPRVCILDGGINKMKLTGLLTVPSPQI

Target ORF information:

RefSeq Version	XM_012962156.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain containing kinase (tbck), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962156.2

ORF Insert Sequence:

ATGAATCGCC ACCGAATGGT CCATAGGGCA CTTGCTCCTC ACAATATTCT TCTAGATGGA 
GAGGGACATG CCAAACTGGC CAAGTTTGGG CTTTATCATA TCACAGATCA TGGGGCTGAT 
GTTGATTTCC CAATAGGATA CCCTTCTTAC CTGGCACCAG AAGTGATTGC TCAGGGGATT 
GTAAAGCCGA CAGACCCCAC ACAGTGCGAG AGGCCTCTGC CTTCAGGTCC AAAATCCGAT 
GTCTGGTCTC TGGGTCTTAT ATTGTTTGAA CTGTGCATGG GTAGAAAATT ATTTCAGAGT 
CTGGATATCT CTGAGAAATT AAAACTAATA CTTACATTAG GTTGTGTTGA CGACATTATA 
ACCGTACTTA GTGAAGAACA TGGGTGTGAA GATATTTTAA AGAGTGTCCC TGAAAATACC 
TTAGCCTTGT TGCGAAAGTG CCTCACGTTT CATCCTACAA AAAGGCCTAC CTCTGCGGAA 
TTGCTTGCTG ACAGCGTGTT CAGTGAAGTG TCCCCTTTGT ACCTCCCTTT TCGCAAACCT 
GTTGGTCTCT TTTCTTCTGC TTTGCGATGT GCTAATCTAA TACTGCCTGA AGATATTAGT 
CTCTTATGCA AAGATGAGGA CAGTGACTTC CTTGCAGAAA GATCTATTGA AGAAGTATAT 
CACTTGTGGT GCTTGGCAGG CGGAGACCTA GAGAAAGAGC TTATAAGCAA GGGCATAATT 
CGATCAAAGC CACCTATCTG CACTTTACCC AACCTTCTCC TTGAAGATGG TGAAAGCTTT 
GGTCAAGGAA GGGACAGGAG CTCCCTGCTG GATGACACCA CTGTGACTCT TTCCTTGTGC 
CAACTCAGAA ATAGATTAAA AGATGTCCCT GGTGAAGCAT ATTATCCATT ACTAGGTGAA 
GACCAATCCA ACATACCACA TTCTGGCAGC AGCAATGAGT TGTCCACCTC AGCCACTCTT 
CCTCTGATTA TTCGTGAGAG AGACACAGAG TATCAGCTAA ACAGAATTGT TTTATTTGAC 
CGGTTGCTAA ATGCATATCC ATACAAGAAA AATATGATTT GGAAAGAGGC CAGAGTGGAT 
ATTCCTCCTC TTTTGAGAGG ATTAACTTGG GCTGCTCTTC TTGGTGTAGA GGGGGCCATT 
CAAGAAAAAT ATGATATGAT AGATAAGGAC ACTCCAATAC CTACAGACCG TCAGATTGAA 
GTAGATATCC CACGTTGCCA TCAGTATGAT GAGCTCCTTT CTTCTCCTGA AGGCCATGCA 
AAATTTCGAA GGGTACTGAA AGCTTGGGTG GTTTCACATC CTAACCTGGT ATATTGGCAA 
GGGTTGGATT CACTGTGTGC ACCCTTCCTC TACCTGAATT TTAACAATGA AGCGCTGGCA 
TATGCCTGTA TGTCTGCCTT CATACCCAAG TACCTGTATA ATTTCTTTTT GAAGGACAAT 
TCCCATGTAA TACAAGAGTA CCTCAAAGTG TTCTCCCAAA TGATTGCCTT TCATGACCCG 
GAACTAAGCA ACCATCTCAA TGAAATTGGC TTTATTCCAG ATCTCTACGC AATTCCTTGG 
TTTCTTACAA TGTTCACGCA TGTTTTTCCT TTGCACAAGA TATTCCATCT CTGGGACACA 
TTGCTGCTGG GGAATGCTTC CTTCCCATTT TGCATTGGAG TGGCCATTTT GCAACAGCTA 
CGAGTCAGGC TTCTTGCGAA TGGTTTTAAT GAATGCATTC TTCTGTTTTC CGATTTGCCA 
GAAATTGACA TTGAGCGCTG TGTCCGTGAG TCTGTTAATT TATTTTGCTG GACACCAAAG 
AGTGCAACAT ACAGACAGCA TGCACAGGTG CCAAAGCAGA TCTCTGAAAG TAATGGGGTG 
AGCAGGACAT CAGCCAGCTT CAGTGTGGAC TTCCAGGAAT CCCCCAAGAC TGACTTGGGC 
CGAGAACCAA TTAACATGAA AGAGCTGAAG TTAGAAGTTT CCCCACGAAT CTCAGCAGAA 
GATCTGATTG ACCTGTGTGA GCTGACCATG CCCAGTATGT CACGGATACC ATCTAAAAAA 
ACCAGATCCA TCAAGCCCAA GCTTTTGGTA GTGGATATTC GACACAGTGA AGATTTCAAT 
CGTGGCCATA TACCTGGAAG CATCAACATG CCGTCTGGCT CTGCATTCAC AACTGAAAGG 
GATTTGGCTC CATGTCCTGC AAGTACCACC CTGCAAAACT TCAAGGGTAA AGTGATTGTG 
ACTGTGGGGC ATGCAGCACA GGATGCATCT GAGTTTGCAG TTAACTTGGT AAAGCTGGGA 
TACCCAAGAG TTTGTATTCT GGATGGCGGC ATTAACAAGA TGAAGCTTAC TGGCTTGCTG 
ACTGTTCCCT CCCCTCAGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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tbck cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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