rims1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following rims1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rims1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa57821 XM_031901442.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OXa30352 XM_031901438.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa30353 XM_031901439.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa30354 XM_031901440.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa44293 XM_018093751.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa44294 XM_018093752.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa44295 XM_018093753.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa44296 XM_018093754.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa44297 XM_018093755.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa57819 XM_031901437.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa57820 XM_031901441.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa30345 XM_031901426.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa30346 XM_031901429.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa30347 XM_031901430.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa30348 XM_031901431.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa30349 XM_031901432.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa30350 XM_031901434.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa44292 XM_018093750.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa57814 XM_031901427.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa57815 XM_031901428.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa57816 XM_031901433.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa57817 XM_031901435.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa57818 XM_031901436.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa57822 XM_031901443.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa23853 NM_001102710.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa57821
    Clone ID Related Accession (Same CDS sequence) XM_031901442.1
    Accession Version XM_031901442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAGAAATA TGTATAAAGG GCAAAATGTA CCTATATGTA TATGCTTATT TGATTCTGGC 
    AACAAGAAAT TAAAAAGTAC TATTCAGAGA AGCACAGAAA CAGGCATGGC AGCAGAAATG
    AGAAGTCGGA TGGTTCGGCA ACCGAGCCGG GAGTCCACAG ATGGAAGTAT TAACAGTTAC
    AGCTCAGAGG GCAATTTAAT TTTTCCAGGG GTGAGACTGG GGGTTGATAG CCAATTCAGC
    GACTTTCTTG ATGGACTAGG ACCAGCACAA CTTGTAGGCA GACAGACATT AGCAACTCCA
    GCAATGGGTG ATATTCAGAT TGGAATGGTA GATAAAAAGG GGCAATTAGA AGTGGAAGTT
    ATACGAGCAA GAGGTCTAAT ACAAAAGCCA GGATCAAAAT CAACTCCAGC TCCGTATGTA
    AAGGTATATC TTTTAGAAAA TGGAGCGTGT GTCGCCAAGA AAAAGACAAG AATTGCACGG
    AAAACCCTTG ATCCTCTTTA TCAACAGACT TTGGTTTTTG AAGAAAGTCC GAATGGAAAG
    GTCCTTCAGG TAATTGTCTG GGGAGATTAT GGTCGAATGG ATCACAAATG CTTCATGGGT
    GTTGCACAGA TTTTATTGGA AGAACTCGAT CTATCAAGTG TGGTGATTGG ATGGTATAAA
    TTGTTTCCGC CATCATCGTT GGTGGATCCA ACGCTGACAC CCCTTACTCG CAGGGCTTCC
    CAATCATCTC TTGAAAGTTC TACAGGTCCT CCTTCCATAC GATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757302.1
    CDS38..805
    Translation

    Target ORF information:

    RefSeq Version XM_031901442.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAGAAATA TGTATAAAGG GCAAAATGTA CCTATATGTA TATGCTTATT TGATTCTGGC 
    AACAAGAAAT TAAAAAGTAC TATTCAGAGA AGCACAGAAA CAGGCATGGC AGCAGAAATG
    AGAAGTCGGA TGGTTCGGCA ACCGAGCCGG GAGTCCACAG ATGGAAGTAT TAACAGTTAC
    AGCTCAGAGG GCAATTTAAT TTTTCCAGGG GTGAGACTGG GGGTTGATAG CCAATTCAGC
    GACTTTCTTG ATGGACTAGG ACCAGCACAA CTTGTAGGCA GACAGACATT AGCAACTCCA
    GCAATGGGTG ATATTCAGAT TGGAATGGTA GATAAAAAGG GGCAATTAGA AGTGGAAGTT
    ATACGAGCAA GAGGTCTAAT ACAAAAGCCA GGATCAAAAT CAACTCCAGC TCCGTATGTA
    AAGGTATATC TTTTAGAAAA TGGAGCGTGT GTCGCCAAGA AAAAGACAAG AATTGCACGG
    AAAACCCTTG ATCCTCTTTA TCAACAGACT TTGGTTTTTG AAGAAAGTCC GAATGGAAAG
    GTCCTTCAGG TAATTGTCTG GGGAGATTAT GGTCGAATGG ATCACAAATG CTTCATGGGT
    GTTGCACAGA TTTTATTGGA AGAACTCGAT CTATCAAGTG TGGTGATTGG ATGGTATAAA
    TTGTTTCCGC CATCATCGTT GGTGGATCCA ACGCTGACAC CCCTTACTCG CAGGGCTTCC
    CAATCATCTC TTGAAAGTTC TACAGGTCCT CCTTCCATAC GATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30352
    Clone ID Related Accession (Same CDS sequence) XM_031901438.1
    Accession Version XM_031901438.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGAAATCACC AACTCGGAAG CCAGTCATCA
    GCTGATTCAT CTTTTAGTAG TCGACGAGGA AGGCAGCTTC CACAAGTCCC AGTAAGAAGC
    AACAGTACAG AACAAGAACC AGAGAGTTTT AGTTCATCAT CGAAAGCAAA TTTATCGATG
    GAAGAACGTA CAAGACAGAT GAAGATAAAA GTTCAACGGT ATAAGCAAAT AGCTGGTTCT
    AGTTCAAGTC AAGAACAGGA ACGAGAGCAA TATACTAAGT ATAACACACA CAGAGATCAG
    TACAGAAGTT GTGACAACGT CTCAGCCAAA TCGTCTGATA GTGATGTCAG TGATGTGTCA
    GCCATTTCCC GTACCAGTAG TGCCTCACGT CTCAGTAGCA CAAGCTTTAT GTCAGAGCAA
    TCTGAACGCC CCCGCAGGAG ACTATGTTCC TTTACACCCA GAATGCAAAG CAGGCGGATG
    GGTACTTCAG GGATAGCCAT AACTAAGAGC ACAAGTGTGA GTGGAGAAAT TTACAAACTG
    GAACACAATG ATGGAAGTCA GTCTGACACA GCCGTTGGGA TGGTTGGGAC CGGTGGAAAG
    AAAAGGAGAT CCAGTCTAAG TGCCAAAGTA GTTGCCATAG TATCACGAAG AAGCAGAAGC
    ACATCCCAGC TTAGCCAAAC AGATTCTGGC AACAAGAAAT TAAAAAGTAC TATTCAGAGA
    AGCACAGAAA CAGGCATGGC AGCAGAAATG AGAAGTCGGA TGGTTCGGCA ACCGAGCCGG
    GAGTCCACAG ATGGAAGTAT TAACAGTTAC AGCTCAGAGG GCAATTTAAT TTTTCCAGGG
    GTGAGACTGG GGGTTGATAG CCAATTCAGC GACTTTCTTG ATGGACTAGG ACCAGCACAA
    CTTGTAGGCA GACAGACATT AGCAACTCCA GCAATGGGTG ATATTCAGAT TGGAATGGTA
    GATAAAAAGG GGCAATTAGA AGTGGAAGTT ATACGAGCAA GAGGTCTAAT ACAAAAGCCA
    GGATCAAAAT CAACTCCAGC TCCGTATGTA AAGGTATATC TTTTAGAAAA TGGAGCGTGT
    GTCGCCAAGA AAAAGACAAG AATTGCACGG AAAACCCTTG ATCCTCTTTA TCAACAGACT
    TTGGTTTTTG AAGAAAGTCC GAATGGAAAG GTCCTTCAGG TAATTGTCTG GGGAGATTAT
    GGTCGAATGG ATCACAAATG CTTCATGGGT GTTGCACAGA TTTTATTGGA AGAACTCGAT
    CTATCAAGTG TGGTGATTGG ATGGTATAAA TTGTTTCCGC CATCATCGTT GGTGGATCCA
    ACGCTGACAC CCCTTACTCG CAGGGCTTCC CAATCATCTC TTGAAAGTTC TACAGGTCCT
    CCTTCCATAC GATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757298.1
    CDS362..4819
    Translation

    Target ORF information:

    RefSeq Version XM_031901438.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901438.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGAAATCACC AACTCGGAAG CCAGTCATCA
    GCTGATTCAT CTTTTAGTAG TCGACGAGGA AGGCAGCTTC CACAAGTCCC AGTAAGAAGC
    AACAGTACAG AACAAGAACC AGAGAGTTTT AGTTCATCAT CGAAAGCAAA TTTATCGATG
    GAAGAACGTA CAAGACAGAT GAAGATAAAA GTTCAACGGT ATAAGCAAAT AGCTGGTTCT
    AGTTCAAGTC AAGAACAGGA ACGAGAGCAA TATACTAAGT ATAACACACA CAGAGATCAG
    TACAGAAGTT GTGACAACGT CTCAGCCAAA TCGTCTGATA GTGATGTCAG TGATGTGTCA
    GCCATTTCCC GTACCAGTAG TGCCTCACGT CTCAGTAGCA CAAGCTTTAT GTCAGAGCAA
    TCTGAACGCC CCCGCAGGAG ACTATGTTCC TTTACACCCA GAATGCAAAG CAGGCGGATG
    GGTACTTCAG GGATAGCCAT AACTAAGAGC ACAAGTGTGA GTGGAGAAAT TTACAAACTG
    GAACACAATG ATGGAAGTCA GTCTGACACA GCCGTTGGGA TGGTTGGGAC CGGTGGAAAG
    AAAAGGAGAT CCAGTCTAAG TGCCAAAGTA GTTGCCATAG TATCACGAAG AAGCAGAAGC
    ACATCCCAGC TTAGCCAAAC AGATTCTGGC AACAAGAAAT TAAAAAGTAC TATTCAGAGA
    AGCACAGAAA CAGGCATGGC AGCAGAAATG AGAAGTCGGA TGGTTCGGCA ACCGAGCCGG
    GAGTCCACAG ATGGAAGTAT TAACAGTTAC AGCTCAGAGG GCAATTTAAT TTTTCCAGGG
    GTGAGACTGG GGGTTGATAG CCAATTCAGC GACTTTCTTG ATGGACTAGG ACCAGCACAA
    CTTGTAGGCA GACAGACATT AGCAACTCCA GCAATGGGTG ATATTCAGAT TGGAATGGTA
    GATAAAAAGG GGCAATTAGA AGTGGAAGTT ATACGAGCAA GAGGTCTAAT ACAAAAGCCA
    GGATCAAAAT CAACTCCAGC TCCGTATGTA AAGGTATATC TTTTAGAAAA TGGAGCGTGT
    GTCGCCAAGA AAAAGACAAG AATTGCACGG AAAACCCTTG ATCCTCTTTA TCAACAGACT
    TTGGTTTTTG AAGAAAGTCC GAATGGAAAG GTCCTTCAGG TAATTGTCTG GGGAGATTAT
    GGTCGAATGG ATCACAAATG CTTCATGGGT GTTGCACAGA TTTTATTGGA AGAACTCGAT
    CTATCAAGTG TGGTGATTGG ATGGTATAAA TTGTTTCCGC CATCATCGTT GGTGGATCCA
    ACGCTGACAC CCCTTACTCG CAGGGCTTCC CAATCATCTC TTGAAAGTTC TACAGGTCCT
    CCTTCCATAC GATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30353
    Clone ID Related Accession (Same CDS sequence) XM_031901439.1
    Accession Version XM_031901439.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGAAATACTG TAAGGCACAG TAAAACATTA
    CCACCCAAGA TGCCTTTACT AGAGTATGGT TCTCACTGGA GACTCTACAG TGAACTGCAG
    CCCTCCCTTG ACAGGGCTAG AAGTGCTAGT ACCAACTGCT TGAGACCAGA TACTAGTTTG
    CATTCACCAG AACGAGAAAG GCAGCCCAGA AAACTGGAAA GATCTAGCAA CCAGAAACAA
    AATAGGAAAA GCACTGTTTC TGAAATAGAA AGAAATCACC AACTCGGAAG CCAGTCATCA
    GCTGATTCAT CTTTTAGTAG TCGACGAGGA AGGCAGCTTC CACAAGTCCC AGTAAGAAGC
    AACAGTACAG AACAAGATTC TGGCAACAAG AAATTAAAAA GTACTATTCA GAGAAGCACA
    GAAACAGGCA TGGCAGCAGA AATGAGAAGT CGGATGGTTC GGCAACCGAG CCGGGAGTCC
    ACAGATGGAA GTATTAACAG TTACAGCTCA GAGGGCAATT TAATTTTTCC AGGGGTGAGA
    CTGGGGGTTG ATAGCCAATT CAGCGACTTT CTTGATGGAC TAGGACCAGC ACAACTTGTA
    GGCAGACAGA CATTAGCAAC TCCAGCAATG GGTGATATTC AGATTGGAAT GGTAGATAAA
    AAGGGGCAAT TAGAAGTGGA AGTTATACGA GCAAGAGGTC TAATACAAAA GCCAGGATCA
    AAATCAACTC CAGCTCCGTA TGTAAAGGTA TATCTTTTAG AAAATGGAGC GTGTGTCGCC
    AAGAAAAAGA CAAGAATTGC ACGGAAAACC CTTGATCCTC TTTATCAACA GACTTTGGTT
    TTTGAAGAAA GTCCGAATGG AAAGGTCCTT CAGGTAATTG TCTGGGGAGA TTATGGTCGA
    ATGGATCACA AATGCTTCAT GGGTGTTGCA CAGATTTTAT TGGAAGAACT CGATCTATCA
    AGTGTGGTGA TTGGATGGTA TAAATTGTTT CCGCCATCAT CGTTGGTGGA TCCAACGCTG
    ACACCCCTTA CTCGCAGGGC TTCCCAATCA TCTCTTGAAA GTTCTACAGG TCCTCCTTCC
    ATACGATCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757299.1
    CDS364..4515
    Translation

    Target ORF information:

    RefSeq Version XM_031901439.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901439.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGAAATACTG TAAGGCACAG TAAAACATTA
    CCACCCAAGA TGCCTTTACT AGAGTATGGT TCTCACTGGA GACTCTACAG TGAACTGCAG
    CCCTCCCTTG ACAGGGCTAG AAGTGCTAGT ACCAACTGCT TGAGACCAGA TACTAGTTTG
    CATTCACCAG AACGAGAAAG GCAGCCCAGA AAACTGGAAA GATCTAGCAA CCAGAAACAA
    AATAGGAAAA GCACTGTTTC TGAAATAGAA AGAAATCACC AACTCGGAAG CCAGTCATCA
    GCTGATTCAT CTTTTAGTAG TCGACGAGGA AGGCAGCTTC CACAAGTCCC AGTAAGAAGC
    AACAGTACAG AACAAGATTC TGGCAACAAG AAATTAAAAA GTACTATTCA GAGAAGCACA
    GAAACAGGCA TGGCAGCAGA AATGAGAAGT CGGATGGTTC GGCAACCGAG CCGGGAGTCC
    ACAGATGGAA GTATTAACAG TTACAGCTCA GAGGGCAATT TAATTTTTCC AGGGGTGAGA
    CTGGGGGTTG ATAGCCAATT CAGCGACTTT CTTGATGGAC TAGGACCAGC ACAACTTGTA
    GGCAGACAGA CATTAGCAAC TCCAGCAATG GGTGATATTC AGATTGGAAT GGTAGATAAA
    AAGGGGCAAT TAGAAGTGGA AGTTATACGA GCAAGAGGTC TAATACAAAA GCCAGGATCA
    AAATCAACTC CAGCTCCGTA TGTAAAGGTA TATCTTTTAG AAAATGGAGC GTGTGTCGCC
    AAGAAAAAGA CAAGAATTGC ACGGAAAACC CTTGATCCTC TTTATCAACA GACTTTGGTT
    TTTGAAGAAA GTCCGAATGG AAAGGTCCTT CAGGTAATTG TCTGGGGAGA TTATGGTCGA
    ATGGATCACA AATGCTTCAT GGGTGTTGCA CAGATTTTAT TGGAAGAACT CGATCTATCA
    AGTGTGGTGA TTGGATGGTA TAAATTGTTT CCGCCATCAT CGTTGGTGGA TCCAACGCTG
    ACACCCCTTA CTCGCAGGGC TTCCCAATCA TCTCTTGAAA GTTCTACAGG TCCTCCTTCC
    ATACGATCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30354
    Clone ID Related Accession (Same CDS sequence) XM_031901440.1
    Accession Version XM_031901440.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGTGAACTGC AGCCCTCCCT TGACAGGGCT
    AGAAGTGCTA GTACCAACTG CTTGAGACCA GATACTAGTT TGCATTCACC AGAACGAGAA
    AGGCAGCCCA GAAAACTGGA AAGATCTAGC AACCAGAAAC AAAATAGGAA AAGCACTGTT
    TCTGAAATAG AAAGAAATCA CCAACTCGGA AGCCAGTCAT CAGCTGATTC ATCTTTTAGT
    AGTCGACGAG GAAGGCAGCT TCCACAAGTC CCAGTAAGAA GCAACAGTAC AGAACAAGAT
    TCTGGCAACA AGAAATTAAA AAGTACTATT CAGAGAAGCA CAGAAACAGG CATGGCAGCA
    GAAATGAGAA GTCGGATGGT TCGGCAACCG AGCCGGGAGT CCACAGATGG AAGTATTAAC
    AGTTACAGCT CAGAGGGCAA TTTAATTTTT CCAGGGGTGA GACTGGGGGT TGATAGCCAA
    TTCAGCGACT TTCTTGATGG ACTAGGACCA GCACAACTTG TAGGCAGACA GACATTAGCA
    ACTCCAGCAA TGGGTGATAT TCAGATTGGA ATGGTAGATA AAAAGGGGCA ATTAGAAGTG
    GAAGTTATAC GAGCAAGAGG TCTAATACAA AAGCCAGGAT CAAAATCAAC TCCAGCTCCG
    TATGTAAAGG TATATCTTTT AGAAAATGGA GCGTGTGTCG CCAAGAAAAA GACAAGAATT
    GCACGGAAAA CCCTTGATCC TCTTTATCAA CAGACTTTGG TTTTTGAAGA AAGTCCGAAT
    GGAAAGGTCC TTCAGGTAAT TGTCTGGGGA GATTATGGTC GAATGGATCA CAAATGCTTC
    ATGGGTGTTG CACAGATTTT ATTGGAAGAA CTCGATCTAT CAAGTGTGGT GATTGGATGG
    TATAAATTGT TTCCGCCATC ATCGTTGGTG GATCCAACGC TGACACCCCT TACTCGCAGG
    GCTTCCCAAT CATCTCTTGA AAGTTCTACA GGTCCTCCTT CCATACGATC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757300.1
    CDS364..4437
    Translation

    Target ORF information:

    RefSeq Version XM_031901440.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901440.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGTGAACTGC AGCCCTCCCT TGACAGGGCT
    AGAAGTGCTA GTACCAACTG CTTGAGACCA GATACTAGTT TGCATTCACC AGAACGAGAA
    AGGCAGCCCA GAAAACTGGA AAGATCTAGC AACCAGAAAC AAAATAGGAA AAGCACTGTT
    TCTGAAATAG AAAGAAATCA CCAACTCGGA AGCCAGTCAT CAGCTGATTC ATCTTTTAGT
    AGTCGACGAG GAAGGCAGCT TCCACAAGTC CCAGTAAGAA GCAACAGTAC AGAACAAGAT
    TCTGGCAACA AGAAATTAAA AAGTACTATT CAGAGAAGCA CAGAAACAGG CATGGCAGCA
    GAAATGAGAA GTCGGATGGT TCGGCAACCG AGCCGGGAGT CCACAGATGG AAGTATTAAC
    AGTTACAGCT CAGAGGGCAA TTTAATTTTT CCAGGGGTGA GACTGGGGGT TGATAGCCAA
    TTCAGCGACT TTCTTGATGG ACTAGGACCA GCACAACTTG TAGGCAGACA GACATTAGCA
    ACTCCAGCAA TGGGTGATAT TCAGATTGGA ATGGTAGATA AAAAGGGGCA ATTAGAAGTG
    GAAGTTATAC GAGCAAGAGG TCTAATACAA AAGCCAGGAT CAAAATCAAC TCCAGCTCCG
    TATGTAAAGG TATATCTTTT AGAAAATGGA GCGTGTGTCG CCAAGAAAAA GACAAGAATT
    GCACGGAAAA CCCTTGATCC TCTTTATCAA CAGACTTTGG TTTTTGAAGA AAGTCCGAAT
    GGAAAGGTCC TTCAGGTAAT TGTCTGGGGA GATTATGGTC GAATGGATCA CAAATGCTTC
    ATGGGTGTTG CACAGATTTT ATTGGAAGAA CTCGATCTAT CAAGTGTGGT GATTGGATGG
    TATAAATTGT TTCCGCCATC ATCGTTGGTG GATCCAACGC TGACACCCCT TACTCGCAGG
    GCTTCCCAAT CATCTCTTGA AAGTTCTACA GGTCCTCCTT CCATACGATC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44293
    Clone ID Related Accession (Same CDS sequence) XM_018093751.1
    Accession Version XM_018093751.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGAAATACT GTAAGGCACA GTAAAACATT ACCACCCAAG
    ATGCCTTTAC TAGAGTATGG TTCTCACTGG AGACTCTACA GTGAACTGCA GCCCTCCCTT
    GACAGGGCTA GAAGTGCTAG TACCAACTGC TTGAGACCAG ATACTAGTTT GCATTCACCA
    GAACGAGAAA GAAATCACCA ACTCGGAAGC CAGTCATCAG CTGATTCATC TTTTAGTAGT
    CGACGAGGAA GGCAGCTTCC ACAAGTCCCA GTAAGAAGCA ACAGTACAGA ACAAGAACCA
    GAGAGTTTTA GTTCATCATC GAAAGCAAAT TTATCGATGG AAGAACGTAC AAGACAGATG
    AAGATAAAAG TTCAACGGTA TAAGCAAATA GCTGGTTCTA GTTCAAGTCA AGAACAGGAA
    CGAGAGCAAT ATACTAAGTA TAACACACAC AGAGATCAGT ACAGAAGTTG TGACAACGTC
    TCAGCCAAAT CGTCTGATAG TGATGTCAGT GATGTGTCAG CCATTTCCCG TACCAGTAGT
    GCCTCACGTC TCAGTAGCAC AAGCTTTATG TCAGAGCAAT CTGAACGCCC CCGCAGGAGA
    CTATGTTCCT TTACACCCAG AATGCAAAGC AGGCGGATGG GTACTTCAGG GATAGCCATA
    ACTAAGAGCA CAAGTGTGAG TGGAGAAATT TACAAACTGG AACACAATGA TGGAAGTCAG
    TCTGACACAG CCGTTGGGAT GGTTGGGACC GGTGGAAAGA AAAGGAGATC CAGTCTAAGT
    GCCAAAGTAG TTGCCATAGT ATCACGAAGA AGCAGAAGCA CATCCCAGCT TAGCCAAACA
    GATTCTGGCA ACAAGAAATT AAAAAGTACT ATTCAGAGAA GCACAGAAAC AGGCATGGCA
    GCAGAAATGA GAAGTCGGAT GGTTCGGCAA CCGAGCCGGG AGTCCACAGA TGGAAGTATT
    AACAGTTACA GCTCAGAGGG CAATTTAATT TTTCCAGGGG TGAGACTGGG GGTTGATAGC
    CAATTCAGCG ACTTTCTTGA TGGACTAGGA CCAGCACAAC TTGTAGGCAG ACAGACATTA
    GCAACTCCAG CAATGGGTGA TATTCAGATT GGAATGGTAG ATAAAAAGGG GCAATTAGAA
    GTGGAAGTTA TACGAGCAAG AGGTCTAATA CAAAAGCCAG GATCAAAATC AACTCCAGCT
    CCGTATGTAA AGGTATATCT TTTAGAAAAT GGAGCGTGTG TCGCCAAGAA AAAGACAAGA
    ATTGCACGGA AAACCCTTGA TCCTCTTTAT CAACAGACTT TGGTTTTTGA AGAAAGTCCG
    AATGGAAAGG TCCTTCAGGT AATTGTCTGG GGAGATTATG GTCGAATGGA TCACAAATGC
    TTCATGGGTG TTGCACAGAT TTTATTGGAA GAACTCGATC TATCAAGTGT GGTGATTGGA
    TGGTATAAAT TGTTTCCGCC ATCATCGTTG GTGGATCCAA CGCTGACACC CCTTACTCGC
    AGGGCTTCCC AATCATCTCT TGAAAGTTCT ACAGGTCCTC CTTCCATACG ATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949240.1
    CDS371..4867
    Translation

    Target ORF information:

    RefSeq Version XM_018093751.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093751.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGAAATACT GTAAGGCACA GTAAAACATT ACCACCCAAG
    ATGCCTTTAC TAGAGTATGG TTCTCACTGG AGACTCTACA GTGAACTGCA GCCCTCCCTT
    GACAGGGCTA GAAGTGCTAG TACCAACTGC TTGAGACCAG ATACTAGTTT GCATTCACCA
    GAACGAGAAA GAAATCACCA ACTCGGAAGC CAGTCATCAG CTGATTCATC TTTTAGTAGT
    CGACGAGGAA GGCAGCTTCC ACAAGTCCCA GTAAGAAGCA ACAGTACAGA ACAAGAACCA
    GAGAGTTTTA GTTCATCATC GAAAGCAAAT TTATCGATGG AAGAACGTAC AAGACAGATG
    AAGATAAAAG TTCAACGGTA TAAGCAAATA GCTGGTTCTA GTTCAAGTCA AGAACAGGAA
    CGAGAGCAAT ATACTAAGTA TAACACACAC AGAGATCAGT ACAGAAGTTG TGACAACGTC
    TCAGCCAAAT CGTCTGATAG TGATGTCAGT GATGTGTCAG CCATTTCCCG TACCAGTAGT
    GCCTCACGTC TCAGTAGCAC AAGCTTTATG TCAGAGCAAT CTGAACGCCC CCGCAGGAGA
    CTATGTTCCT TTACACCCAG AATGCAAAGC AGGCGGATGG GTACTTCAGG GATAGCCATA
    ACTAAGAGCA CAAGTGTGAG TGGAGAAATT TACAAACTGG AACACAATGA TGGAAGTCAG
    TCTGACACAG CCGTTGGGAT GGTTGGGACC GGTGGAAAGA AAAGGAGATC CAGTCTAAGT
    GCCAAAGTAG TTGCCATAGT ATCACGAAGA AGCAGAAGCA CATCCCAGCT TAGCCAAACA
    GATTCTGGCA ACAAGAAATT AAAAAGTACT ATTCAGAGAA GCACAGAAAC AGGCATGGCA
    GCAGAAATGA GAAGTCGGAT GGTTCGGCAA CCGAGCCGGG AGTCCACAGA TGGAAGTATT
    AACAGTTACA GCTCAGAGGG CAATTTAATT TTTCCAGGGG TGAGACTGGG GGTTGATAGC
    CAATTCAGCG ACTTTCTTGA TGGACTAGGA CCAGCACAAC TTGTAGGCAG ACAGACATTA
    GCAACTCCAG CAATGGGTGA TATTCAGATT GGAATGGTAG ATAAAAAGGG GCAATTAGAA
    GTGGAAGTTA TACGAGCAAG AGGTCTAATA CAAAAGCCAG GATCAAAATC AACTCCAGCT
    CCGTATGTAA AGGTATATCT TTTAGAAAAT GGAGCGTGTG TCGCCAAGAA AAAGACAAGA
    ATTGCACGGA AAACCCTTGA TCCTCTTTAT CAACAGACTT TGGTTTTTGA AGAAAGTCCG
    AATGGAAAGG TCCTTCAGGT AATTGTCTGG GGAGATTATG GTCGAATGGA TCACAAATGC
    TTCATGGGTG TTGCACAGAT TTTATTGGAA GAACTCGATC TATCAAGTGT GGTGATTGGA
    TGGTATAAAT TGTTTCCGCC ATCATCGTTG GTGGATCCAA CGCTGACACC CCTTACTCGC
    AGGGCTTCCC AATCATCTCT TGAAAGTTCT ACAGGTCCTC CTTCCATACG ATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44294
    Clone ID Related Accession (Same CDS sequence) XM_018093752.1
    Accession Version XM_018093752.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGTGAACTG CAGCCCTCCC TTGACAGGGC TAGAAGTGCT
    AGTACCAACT GCTTGAGACC AGATACTAGT TTGCATTCAC CAGAACGAGA AAGGCAGCCC
    AGAAAACTGG AAAGATCTAG CAACCAGAAA CAAAATAGGA AAAGCACTGT TTCTGAAATA
    GAAAGAAATC ACCAACTCGG AAGCCAGTCA TCAGCTGATT CATCTTTTAG TAGTCGACGA
    GGAAGGCAGC TTCCACAAGT CCCAGTAAGA AGCAACAGTA CAGAACAAGA ACCAGAGAGT
    TTTAGTTCAT CATCGAAAGC AAATTTATCG ATGGAAGAAC GTACAAGACA GATGAAGATA
    AAAGTTCAAC GGTATAAGCA AATAGCTGGT TCTAGTTCAA GTCAAGAACA GGAACGAGAG
    CAATATACTA AGTATAACAC ACACAGAGAT CAGTACAGAA GTTGTGACAA CGTCTCAGCC
    AAATCGTCTG ATAGTGATGT CAGTGATGTG TCAGCCATTT CCCGTACCAG TAGTGCCTCA
    CGTCTCAGTA GCACAAGCTT TATGTCAGAG CAATCTGAAC GCCCCCGCAG GAGACTATGT
    TCCTTTACAC CCAGAATGCA AAGCAGGCGG ATGGGTACTT CAGGGATAGC CATAACTAAG
    AGCACAAGTG TGAGTGGAGA AATTTACAAA CTGGAACACA ATGATGGAAG TCAGTCTGAC
    ACAGCCGTTG GGATGGTTGG GACCGGTGGA AAGAAAAGGA GATCCAGTCT AAGTGCCAAA
    GTAGTTGCCA TAGTATCACG AAGAAGCAGA AGCACATCCC AGCTTAGCCA AACAGATTCT
    GGCAACAAGA AATTAAAAAG TACTATTCAG AGAAGCACAG AAACAGGCAT GGCAGCAGAA
    ATGAGAAGTC GGATGGTTCG GCAACCGAGC CGGGAGTCCA CAGATGGAAG TATTAACAGT
    TACAGCTCAG AGGGCAATTT AATTTTTCCA GGGGTGAGAC TGGGGGTTGA TAGCCAATTC
    AGCGACTTTC TTGATGGACT AGGACCAGCA CAACTTGTAG GCAGACAGAC ATTAGCAACT
    CCAGCAATGG GTGATATTCA GATTGGAATG GTAGATAAAA AGGGGCAATT AGAAGTGGAA
    GTTATACGAG CAAGAGGTCT AATACAAAAG CCAGGATCAA AATCAACTCC AGCTCCGTAT
    GTAAAGGTAT ATCTTTTAGA AAATGGAGCG TGTGTCGCCA AGAAAAAGAC AAGAATTGCA
    CGGAAAACCC TTGATCCTCT TTATCAACAG ACTTTGGTTT TTGAAGAAAG TCCGAATGGA
    AAGGTCCTTC AGGTAATTGT CTGGGGAGAT TATGGTCGAA TGGATCACAA ATGCTTCATG
    GGTGTTGCAC AGATTTTATT GGAAGAACTC GATCTATCAA GTGTGGTGAT TGGATGGTAT
    AAATTGTTTC CGCCATCATC GTTGGTGGAT CCAACGCTGA CACCCCTTAC TCGCAGGGCT
    TCCCAATCAT CTCTTGAAAG TTCTACAGGT CCTCCTTCCA TACGATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949241.1
    CDS371..4861
    Translation

    Target ORF information:

    RefSeq Version XM_018093752.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGTGAACTG CAGCCCTCCC TTGACAGGGC TAGAAGTGCT
    AGTACCAACT GCTTGAGACC AGATACTAGT TTGCATTCAC CAGAACGAGA AAGGCAGCCC
    AGAAAACTGG AAAGATCTAG CAACCAGAAA CAAAATAGGA AAAGCACTGT TTCTGAAATA
    GAAAGAAATC ACCAACTCGG AAGCCAGTCA TCAGCTGATT CATCTTTTAG TAGTCGACGA
    GGAAGGCAGC TTCCACAAGT CCCAGTAAGA AGCAACAGTA CAGAACAAGA ACCAGAGAGT
    TTTAGTTCAT CATCGAAAGC AAATTTATCG ATGGAAGAAC GTACAAGACA GATGAAGATA
    AAAGTTCAAC GGTATAAGCA AATAGCTGGT TCTAGTTCAA GTCAAGAACA GGAACGAGAG
    CAATATACTA AGTATAACAC ACACAGAGAT CAGTACAGAA GTTGTGACAA CGTCTCAGCC
    AAATCGTCTG ATAGTGATGT CAGTGATGTG TCAGCCATTT CCCGTACCAG TAGTGCCTCA
    CGTCTCAGTA GCACAAGCTT TATGTCAGAG CAATCTGAAC GCCCCCGCAG GAGACTATGT
    TCCTTTACAC CCAGAATGCA AAGCAGGCGG ATGGGTACTT CAGGGATAGC CATAACTAAG
    AGCACAAGTG TGAGTGGAGA AATTTACAAA CTGGAACACA ATGATGGAAG TCAGTCTGAC
    ACAGCCGTTG GGATGGTTGG GACCGGTGGA AAGAAAAGGA GATCCAGTCT AAGTGCCAAA
    GTAGTTGCCA TAGTATCACG AAGAAGCAGA AGCACATCCC AGCTTAGCCA AACAGATTCT
    GGCAACAAGA AATTAAAAAG TACTATTCAG AGAAGCACAG AAACAGGCAT GGCAGCAGAA
    ATGAGAAGTC GGATGGTTCG GCAACCGAGC CGGGAGTCCA CAGATGGAAG TATTAACAGT
    TACAGCTCAG AGGGCAATTT AATTTTTCCA GGGGTGAGAC TGGGGGTTGA TAGCCAATTC
    AGCGACTTTC TTGATGGACT AGGACCAGCA CAACTTGTAG GCAGACAGAC ATTAGCAACT
    CCAGCAATGG GTGATATTCA GATTGGAATG GTAGATAAAA AGGGGCAATT AGAAGTGGAA
    GTTATACGAG CAAGAGGTCT AATACAAAAG CCAGGATCAA AATCAACTCC AGCTCCGTAT
    GTAAAGGTAT ATCTTTTAGA AAATGGAGCG TGTGTCGCCA AGAAAAAGAC AAGAATTGCA
    CGGAAAACCC TTGATCCTCT TTATCAACAG ACTTTGGTTT TTGAAGAAAG TCCGAATGGA
    AAGGTCCTTC AGGTAATTGT CTGGGGAGAT TATGGTCGAA TGGATCACAA ATGCTTCATG
    GGTGTTGCAC AGATTTTATT GGAAGAACTC GATCTATCAA GTGTGGTGAT TGGATGGTAT
    AAATTGTTTC CGCCATCATC GTTGGTGGAT CCAACGCTGA CACCCCTTAC TCGCAGGGCT
    TCCCAATCAT CTCTTGAAAG TTCTACAGGT CCTCCTTCCA TACGATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44295
    Clone ID Related Accession (Same CDS sequence) XM_018093753.1
    Accession Version XM_018093753.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGTGAACTG CAGCCCTCCC TTGACAGGGC TAGAAGTGCT
    AGTACCAACT GCTTGAGACC AGATACTAGT TTGCATTCAC CAGAACGAGA AAGAAATCAC
    CAACTCGGAA GCCAGTCATC AGCTGATTCA TCTTTTAGTA GTCGACGAGG AAGGCAGCTT
    CCACAAGTCC CAGTAAGAAG CAACAGTACA GAACAAGAAC CAGAGAGTTT TAGTTCATCA
    TCGAAAGCAA ATTTATCGAT GGAAGAACGT ACAAGACAGA TGAAGATAAA AGTTCAACGG
    TATAAGCAAA TAGCTGGTTC TAGTTCAAGT CAAGAACAGG AACGAGAGCA ATATACTAAG
    TATAACACAC ACAGAGATCA GTACAGAAGT TGTGACAACG TCTCAGCCAA ATCGTCTGAT
    AGTGATGTCA GTGATGTGTC AGCCATTTCC CGTACCAGTA GTGCCTCACG TCTCAGTAGC
    ACAAGCTTTA TGTCAGAGCA ATCTGAACGC CCCCGCAGGA GACTATGTTC CTTTACACCC
    AGAATGCAAA GCAGGCGGAT GGGTACTTCA GGGATAGCCA TAACTAAGAG CACAAGTGTG
    AGTGGAGAAA TTTACAAACT GGAACACAAT GATGGAAGTC AGTCTGACAC AGCCGTTGGG
    ATGGTTGGGA CCGGTGGAAA GAAAAGGAGA TCCAGTCTAA GTGCCAAAGT AGTTGCCATA
    GTATCACGAA GAAGCAGAAG CACATCCCAG CTTAGCCAAA CAGATTCTGG CAACAAGAAA
    TTAAAAAGTA CTATTCAGAG AAGCACAGAA ACAGGCATGG CAGCAGAAAT GAGAAGTCGG
    ATGGTTCGGC AACCGAGCCG GGAGTCCACA GATGGAAGTA TTAACAGTTA CAGCTCAGAG
    GGCAATTTAA TTTTTCCAGG GGTGAGACTG GGGGTTGATA GCCAATTCAG CGACTTTCTT
    GATGGACTAG GACCAGCACA ACTTGTAGGC AGACAGACAT TAGCAACTCC AGCAATGGGT
    GATATTCAGA TTGGAATGGT AGATAAAAAG GGGCAATTAG AAGTGGAAGT TATACGAGCA
    AGAGGTCTAA TACAAAAGCC AGGATCAAAA TCAACTCCAG CTCCGTATGT AAAGGTATAT
    CTTTTAGAAA ATGGAGCGTG TGTCGCCAAG AAAAAGACAA GAATTGCACG GAAAACCCTT
    GATCCTCTTT ATCAACAGAC TTTGGTTTTT GAAGAAAGTC CGAATGGAAA GGTCCTTCAG
    GTAATTGTCT GGGGAGATTA TGGTCGAATG GATCACAAAT GCTTCATGGG TGTTGCACAG
    ATTTTATTGG AAGAACTCGA TCTATCAAGT GTGGTGATTG GATGGTATAA ATTGTTTCCG
    CCATCATCGT TGGTGGATCC AACGCTGACA CCCCTTACTC GCAGGGCTTC CCAATCATCT
    CTTGAAAGTT CTACAGGTCC TCCTTCCATA CGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949242.1
    CDS371..4789
    Translation

    Target ORF information:

    RefSeq Version XM_018093753.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGTGAACTG CAGCCCTCCC TTGACAGGGC TAGAAGTGCT
    AGTACCAACT GCTTGAGACC AGATACTAGT TTGCATTCAC CAGAACGAGA AAGAAATCAC
    CAACTCGGAA GCCAGTCATC AGCTGATTCA TCTTTTAGTA GTCGACGAGG AAGGCAGCTT
    CCACAAGTCC CAGTAAGAAG CAACAGTACA GAACAAGAAC CAGAGAGTTT TAGTTCATCA
    TCGAAAGCAA ATTTATCGAT GGAAGAACGT ACAAGACAGA TGAAGATAAA AGTTCAACGG
    TATAAGCAAA TAGCTGGTTC TAGTTCAAGT CAAGAACAGG AACGAGAGCA ATATACTAAG
    TATAACACAC ACAGAGATCA GTACAGAAGT TGTGACAACG TCTCAGCCAA ATCGTCTGAT
    AGTGATGTCA GTGATGTGTC AGCCATTTCC CGTACCAGTA GTGCCTCACG TCTCAGTAGC
    ACAAGCTTTA TGTCAGAGCA ATCTGAACGC CCCCGCAGGA GACTATGTTC CTTTACACCC
    AGAATGCAAA GCAGGCGGAT GGGTACTTCA GGGATAGCCA TAACTAAGAG CACAAGTGTG
    AGTGGAGAAA TTTACAAACT GGAACACAAT GATGGAAGTC AGTCTGACAC AGCCGTTGGG
    ATGGTTGGGA CCGGTGGAAA GAAAAGGAGA TCCAGTCTAA GTGCCAAAGT AGTTGCCATA
    GTATCACGAA GAAGCAGAAG CACATCCCAG CTTAGCCAAA CAGATTCTGG CAACAAGAAA
    TTAAAAAGTA CTATTCAGAG AAGCACAGAA ACAGGCATGG CAGCAGAAAT GAGAAGTCGG
    ATGGTTCGGC AACCGAGCCG GGAGTCCACA GATGGAAGTA TTAACAGTTA CAGCTCAGAG
    GGCAATTTAA TTTTTCCAGG GGTGAGACTG GGGGTTGATA GCCAATTCAG CGACTTTCTT
    GATGGACTAG GACCAGCACA ACTTGTAGGC AGACAGACAT TAGCAACTCC AGCAATGGGT
    GATATTCAGA TTGGAATGGT AGATAAAAAG GGGCAATTAG AAGTGGAAGT TATACGAGCA
    AGAGGTCTAA TACAAAAGCC AGGATCAAAA TCAACTCCAG CTCCGTATGT AAAGGTATAT
    CTTTTAGAAA ATGGAGCGTG TGTCGCCAAG AAAAAGACAA GAATTGCACG GAAAACCCTT
    GATCCTCTTT ATCAACAGAC TTTGGTTTTT GAAGAAAGTC CGAATGGAAA GGTCCTTCAG
    GTAATTGTCT GGGGAGATTA TGGTCGAATG GATCACAAAT GCTTCATGGG TGTTGCACAG
    ATTTTATTGG AAGAACTCGA TCTATCAAGT GTGGTGATTG GATGGTATAA ATTGTTTCCG
    CCATCATCGT TGGTGGATCC AACGCTGACA CCCCTTACTC GCAGGGCTTC CCAATCATCT
    CTTGAAAGTT CTACAGGTCC TCCTTCCATA CGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44296
    Clone ID Related Accession (Same CDS sequence) XM_018093754.1
    Accession Version XM_018093754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGGCAGCCC AGAAAACTGG AAAGATCTAG CAACCAGAAA
    CAAAATAGGA AAAGCACTGT TTCTGAAATA GAAAGAAATC ACCAACTCGG AAGCCAGTCA
    TCAGCTGATT CATCTTTTAG TAGTCGACGA GGAAGGCAGC TTCCACAAGT CCCAGTAAGA
    AGCAACAGTA CAGAACAAGA ACCAGAGAGT TTTAGTTCAT CATCGAAAGC AAATTTATCG
    ATGGAAGAAC GTACAAGACA GATGAAGATA AAAGTTCAAC GGTATAAGCA AATAGCTGGT
    TCTAGTTCAA GTCAAGAACA GGAACGAGAG CAATATACTA AGTATAACAC ACACAGAGAT
    CAGTACAGAA GTTGTGACAA CGTCTCAGCC AAATCGTCTG ATAGTGATGT CAGTGATGTG
    TCAGCCATTT CCCGTACCAG TAGTGCCTCA CGTCTCAGTA GCACAAGCTT TATGTCAGAG
    CAATCTGAAC GCCCCCGCAG GAGACTATGT TCCTTTACAC CCAGAATGCA AAGCAGGCGG
    ATGGGTACTT CAGGGATAGC CATAACTAAG AGCACAAGTG TGAGTGGAGA AATTTACAAA
    CTGGAACACA ATGATGGAAG TCAGTCTGAC ACAGCCGTTG GGATGGTTGG GACCGGTGGA
    AAGAAAAGGA GATCCAGTCT AAGTGCCAAA GTAGTTGCCA TAGTATCACG AAGAAGCAGA
    AGCACATCCC AGCTTAGCCA AACAGATTCT GGCAACAAGA AATTAAAAAG TACTATTCAG
    AGAAGCACAG AAACAGGCAT GGCAGCAGAA ATGAGAAGTC GGATGGTTCG GCAACCGAGC
    CGGGAGTCCA CAGATGGAAG TATTAACAGT TACAGCTCAG AGGGCAATTT AATTTTTCCA
    GGGGTGAGAC TGGGGGTTGA TAGCCAATTC AGCGACTTTC TTGATGGACT AGGACCAGCA
    CAACTTGTAG GCAGACAGAC ATTAGCAACT CCAGCAATGG GTGATATTCA GATTGGAATG
    GTAGATAAAA AGGGGCAATT AGAAGTGGAA GTTATACGAG CAAGAGGTCT AATACAAAAG
    CCAGGATCAA AATCAACTCC AGCTCCGTAT GTAAAGGTAT ATCTTTTAGA AAATGGAGCG
    TGTGTCGCCA AGAAAAAGAC AAGAATTGCA CGGAAAACCC TTGATCCTCT TTATCAACAG
    ACTTTGGTTT TTGAAGAAAG TCCGAATGGA AAGGTCCTTC AGGTAATTGT CTGGGGAGAT
    TATGGTCGAA TGGATCACAA ATGCTTCATG GGTGTTGCAC AGATTTTATT GGAAGAACTC
    GATCTATCAA GTGTGGTGAT TGGATGGTAT AAATTGTTTC CGCCATCATC GTTGGTGGAT
    CCAACGCTGA CACCCCTTAC TCGCAGGGCT TCCCAATCAT CTCTTGAAAG TTCTACAGGT
    CCTCCTTCCA TACGATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949243.1
    CDS371..4771
    Translation

    Target ORF information:

    RefSeq Version XM_018093754.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGGCAGCCC AGAAAACTGG AAAGATCTAG CAACCAGAAA
    CAAAATAGGA AAAGCACTGT TTCTGAAATA GAAAGAAATC ACCAACTCGG AAGCCAGTCA
    TCAGCTGATT CATCTTTTAG TAGTCGACGA GGAAGGCAGC TTCCACAAGT CCCAGTAAGA
    AGCAACAGTA CAGAACAAGA ACCAGAGAGT TTTAGTTCAT CATCGAAAGC AAATTTATCG
    ATGGAAGAAC GTACAAGACA GATGAAGATA AAAGTTCAAC GGTATAAGCA AATAGCTGGT
    TCTAGTTCAA GTCAAGAACA GGAACGAGAG CAATATACTA AGTATAACAC ACACAGAGAT
    CAGTACAGAA GTTGTGACAA CGTCTCAGCC AAATCGTCTG ATAGTGATGT CAGTGATGTG
    TCAGCCATTT CCCGTACCAG TAGTGCCTCA CGTCTCAGTA GCACAAGCTT TATGTCAGAG
    CAATCTGAAC GCCCCCGCAG GAGACTATGT TCCTTTACAC CCAGAATGCA AAGCAGGCGG
    ATGGGTACTT CAGGGATAGC CATAACTAAG AGCACAAGTG TGAGTGGAGA AATTTACAAA
    CTGGAACACA ATGATGGAAG TCAGTCTGAC ACAGCCGTTG GGATGGTTGG GACCGGTGGA
    AAGAAAAGGA GATCCAGTCT AAGTGCCAAA GTAGTTGCCA TAGTATCACG AAGAAGCAGA
    AGCACATCCC AGCTTAGCCA AACAGATTCT GGCAACAAGA AATTAAAAAG TACTATTCAG
    AGAAGCACAG AAACAGGCAT GGCAGCAGAA ATGAGAAGTC GGATGGTTCG GCAACCGAGC
    CGGGAGTCCA CAGATGGAAG TATTAACAGT TACAGCTCAG AGGGCAATTT AATTTTTCCA
    GGGGTGAGAC TGGGGGTTGA TAGCCAATTC AGCGACTTTC TTGATGGACT AGGACCAGCA
    CAACTTGTAG GCAGACAGAC ATTAGCAACT CCAGCAATGG GTGATATTCA GATTGGAATG
    GTAGATAAAA AGGGGCAATT AGAAGTGGAA GTTATACGAG CAAGAGGTCT AATACAAAAG
    CCAGGATCAA AATCAACTCC AGCTCCGTAT GTAAAGGTAT ATCTTTTAGA AAATGGAGCG
    TGTGTCGCCA AGAAAAAGAC AAGAATTGCA CGGAAAACCC TTGATCCTCT TTATCAACAG
    ACTTTGGTTT TTGAAGAAAG TCCGAATGGA AAGGTCCTTC AGGTAATTGT CTGGGGAGAT
    TATGGTCGAA TGGATCACAA ATGCTTCATG GGTGTTGCAC AGATTTTATT GGAAGAACTC
    GATCTATCAA GTGTGGTGAT TGGATGGTAT AAATTGTTTC CGCCATCATC GTTGGTGGAT
    CCAACGCTGA CACCCCTTAC TCGCAGGGCT TCCCAATCAT CTCTTGAAAG TTCTACAGGT
    CCTCCTTCCA TACGATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44297
    Clone ID Related Accession (Same CDS sequence) XM_018093755.1
    Accession Version XM_018093755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4329bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGAAATCAC CAACTCGGAA GCCAGTCATC AGCTGATTCA
    TCTTTTAGTA GTCGACGAGG AAGGCAGCTT CCACAAGTCC CAGTAAGAAG CAACAGTACA
    GAACAAGAAC CAGAGAGTTT TAGTTCATCA TCGAAAGCAA ATTTATCGAT GGAAGAACGT
    ACAAGACAGA TGAAGATAAA AGTTCAACGG TATAAGCAAA TAGCTGGTTC TAGTTCAAGT
    CAAGAACAGG AACGAGAGCA ATATACTAAG TATAACACAC ACAGAGATCA GTACAGAAGT
    TGTGACAACG TCTCAGCCAA ATCGTCTGAT AGTGATGTCA GTGATGTGTC AGCCATTTCC
    CGTACCAGTA GTGCCTCACG TCTCAGTAGC ACAAGCTTTA TGTCAGAGCA ATCTGAACGC
    CCCCGCAGGA GACTATGTTC CTTTACACCC AGAATGCAAA GCAGGCGGAT GGGTACTTCA
    GGGATAGCCA TAACTAAGAG CACAAGTGTG AGTGGAGAAA TTTACAAACT GGAACACAAT
    GATGGAAGTC AGTCTGACAC AGCCGTTGGG ATGGTTGGGA CCGGTGGAAA GAAAAGGAGA
    TCCAGTCTAA GTGCCAAAGT AGTTGCCATA GTATCACGAA GAAGCAGAAG CACATCCCAG
    CTTAGCCAAA CAGATTCTGG CAACAAGAAA TTAAAAAGTA CTATTCAGAG AAGCACAGAA
    ACAGGCATGG CAGCAGAAAT GAGAAGTCGG ATGGTTCGGC AACCGAGCCG GGAGTCCACA
    GATGGAAGTA TTAACAGTTA CAGCTCAGAG GGCAATTTAA TTTTTCCAGG GGTGAGACTG
    GGGGTTGATA GCCAATTCAG CGACTTTCTT GATGGACTAG GACCAGCACA ACTTGTAGGC
    AGACAGACAT TAGCAACTCC AGCAATGGGT GATATTCAGA TTGGAATGGT AGATAAAAAG
    GGGCAATTAG AAGTGGAAGT TATACGAGCA AGAGGTCTAA TACAAAAGCC AGGATCAAAA
    TCAACTCCAG CTCCGTATGT AAAGGTATAT CTTTTAGAAA ATGGAGCGTG TGTCGCCAAG
    AAAAAGACAA GAATTGCACG GAAAACCCTT GATCCTCTTT ATCAACAGAC TTTGGTTTTT
    GAAGAAAGTC CGAATGGAAA GGTCCTTCAG GTAATTGTCT GGGGAGATTA TGGTCGAATG
    GATCACAAAT GCTTCATGGG TGTTGCACAG ATTTTATTGG AAGAACTCGA TCTATCAAGT
    GTGGTGATTG GATGGTATAA ATTGTTTCCG CCATCATCGT TGGTGGATCC AACGCTGACA
    CCCCTTACTC GCAGGGCTTC CCAATCATCT CTTGAAAGTT CTACAGGTCC TCCTTCCATA
    CGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949244.1
    CDS371..4699
    Translation

    Target ORF information:

    RefSeq Version XM_018093755.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGAAATCAC CAACTCGGAA GCCAGTCATC AGCTGATTCA
    TCTTTTAGTA GTCGACGAGG AAGGCAGCTT CCACAAGTCC CAGTAAGAAG CAACAGTACA
    GAACAAGAAC CAGAGAGTTT TAGTTCATCA TCGAAAGCAA ATTTATCGAT GGAAGAACGT
    ACAAGACAGA TGAAGATAAA AGTTCAACGG TATAAGCAAA TAGCTGGTTC TAGTTCAAGT
    CAAGAACAGG AACGAGAGCA ATATACTAAG TATAACACAC ACAGAGATCA GTACAGAAGT
    TGTGACAACG TCTCAGCCAA ATCGTCTGAT AGTGATGTCA GTGATGTGTC AGCCATTTCC
    CGTACCAGTA GTGCCTCACG TCTCAGTAGC ACAAGCTTTA TGTCAGAGCA ATCTGAACGC
    CCCCGCAGGA GACTATGTTC CTTTACACCC AGAATGCAAA GCAGGCGGAT GGGTACTTCA
    GGGATAGCCA TAACTAAGAG CACAAGTGTG AGTGGAGAAA TTTACAAACT GGAACACAAT
    GATGGAAGTC AGTCTGACAC AGCCGTTGGG ATGGTTGGGA CCGGTGGAAA GAAAAGGAGA
    TCCAGTCTAA GTGCCAAAGT AGTTGCCATA GTATCACGAA GAAGCAGAAG CACATCCCAG
    CTTAGCCAAA CAGATTCTGG CAACAAGAAA TTAAAAAGTA CTATTCAGAG AAGCACAGAA
    ACAGGCATGG CAGCAGAAAT GAGAAGTCGG ATGGTTCGGC AACCGAGCCG GGAGTCCACA
    GATGGAAGTA TTAACAGTTA CAGCTCAGAG GGCAATTTAA TTTTTCCAGG GGTGAGACTG
    GGGGTTGATA GCCAATTCAG CGACTTTCTT GATGGACTAG GACCAGCACA ACTTGTAGGC
    AGACAGACAT TAGCAACTCC AGCAATGGGT GATATTCAGA TTGGAATGGT AGATAAAAAG
    GGGCAATTAG AAGTGGAAGT TATACGAGCA AGAGGTCTAA TACAAAAGCC AGGATCAAAA
    TCAACTCCAG CTCCGTATGT AAAGGTATAT CTTTTAGAAA ATGGAGCGTG TGTCGCCAAG
    AAAAAGACAA GAATTGCACG GAAAACCCTT GATCCTCTTT ATCAACAGAC TTTGGTTTTT
    GAAGAAAGTC CGAATGGAAA GGTCCTTCAG GTAATTGTCT GGGGAGATTA TGGTCGAATG
    GATCACAAAT GCTTCATGGG TGTTGCACAG ATTTTATTGG AAGAACTCGA TCTATCAAGT
    GTGGTGATTG GATGGTATAA ATTGTTTCCG CCATCATCGT TGGTGGATCC AACGCTGACA
    CCCCTTACTC GCAGGGCTTC CCAATCATCT CTTGAAAGTT CTACAGGTCC TCCTTCCATA
    CGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57819
    Clone ID Related Accession (Same CDS sequence) XM_031901437.1
    Accession Version XM_031901437.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGGCAGCCCA GAAAACTGGA AAGATCTAGC
    AACCAGAAAC AAAATAGGAA AAGCACTGTT TCTGAAATAG AAAGAAATCA CCAACTCGGA
    AGCCAGTCAT CAGCTGATTC ATCTTTTAGT AGTCGACGAG GAAGGCAGCT TCCACAAGTC
    CCAGTAAGAA GCAACAGTAC AGAACAAGCA AATTTATCGA TGGAAGAACG TACAAGACAG
    ATGAAGATAA AAGTTCAACG GTATAAGCAA ATAGCTGGTT CTAGTTCAAG TCAAGAACAG
    GAACGAGAGC AATATACTAA GTATAACACA CACAGAGATC AGTACAGAAG TTGTGACAAC
    GTCTCAGCCA AATCGTCTGA TAGTGATGTC AGTGATGTGT CAGCCATTTC CCGTACCAGT
    AGTGCCTCAC GTCTCAGTAG CACAAGCTTT ATGTCAGAGC AATCTGAACG CCCCCGCAGG
    AGACTATGTT CCTTTACACC CAGAATGCAA AGCAGGCGGA TGGGTACTTC AGGGATAGCC
    ATAACTAAGA GCACAAGTGT GAGTGGAGAA ATTTACAAAC TGGAACACAA TGATGGAAGT
    CAGTCTGACA CAGCCGTTGG GATGGTTGGG ACCGGTGGAA AGAAAAGGAG ATCCAGTCTA
    AGTGCCAAAG TAGTTGCCAT AGTATCACGA AGAAGCAGAA GCACATCCCA GCTTAGCCAA
    ACAGATTCTG GCAACAAGAA ATTAAAAAGT ACTATTCAGA GAAGCACAGA AACAGGCATG
    GCAGCAGAAA TGAGAAGTCG GATGGTTCGG CAACCGAGCC GGGAGTCCAC AGATGGAAGT
    ATTAACAGTT ACAGCTCAGA GGGCAATTTA ATTTTTCCAG GGGTGAGACT GGGGGTTGAT
    AGCCAATTCA GCGACTTTCT TGATGGACTA GGACCAGCAC AACTTGTAGG CAGACAGACA
    TTAGCAACTC CAGCAATGGG TGATATTCAG ATTGGAATGG TAGATAAAAA GGGGCAATTA
    GAAGTGGAAG TTATACGAGC AAGAGGTCTA ATACAAAAGC CAGGATCAAA ATCAACTCCA
    GCTCCGTATG TAAAGGTATA TCTTTTAGAA AATGGAGCGT GTGTCGCCAA GAAAAAGACA
    AGAATTGCAC GGAAAACCCT TGATCCTCTT TATCAACAGA CTTTGGTTTT TGAAGAAAGT
    CCGAATGGAA AGGTCCTTCA GGTAATTGTC TGGGGAGATT ATGGTCGAAT GGATCACAAA
    TGCTTCATGG GTGTTGCACA GATTTTATTG GAAGAACTCG ATCTATCAAG TGTGGTGATT
    GGATGGTATA AATTGTTTCC GCCATCATCG TTGGTGGATC CAACGCTGAC ACCCCTTACT
    CGCAGGGCTT CCCAATCATC TCTTGAAAGT TCTACAGGTC CTCCTTCCAT ACGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757297.1
    CDS362..4861
    Translation

    Target ORF information:

    RefSeq Version XM_031901437.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901437.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGGCAGCCCA GAAAACTGGA AAGATCTAGC
    AACCAGAAAC AAAATAGGAA AAGCACTGTT TCTGAAATAG AAAGAAATCA CCAACTCGGA
    AGCCAGTCAT CAGCTGATTC ATCTTTTAGT AGTCGACGAG GAAGGCAGCT TCCACAAGTC
    CCAGTAAGAA GCAACAGTAC AGAACAAGCA AATTTATCGA TGGAAGAACG TACAAGACAG
    ATGAAGATAA AAGTTCAACG GTATAAGCAA ATAGCTGGTT CTAGTTCAAG TCAAGAACAG
    GAACGAGAGC AATATACTAA GTATAACACA CACAGAGATC AGTACAGAAG TTGTGACAAC
    GTCTCAGCCA AATCGTCTGA TAGTGATGTC AGTGATGTGT CAGCCATTTC CCGTACCAGT
    AGTGCCTCAC GTCTCAGTAG CACAAGCTTT ATGTCAGAGC AATCTGAACG CCCCCGCAGG
    AGACTATGTT CCTTTACACC CAGAATGCAA AGCAGGCGGA TGGGTACTTC AGGGATAGCC
    ATAACTAAGA GCACAAGTGT GAGTGGAGAA ATTTACAAAC TGGAACACAA TGATGGAAGT
    CAGTCTGACA CAGCCGTTGG GATGGTTGGG ACCGGTGGAA AGAAAAGGAG ATCCAGTCTA
    AGTGCCAAAG TAGTTGCCAT AGTATCACGA AGAAGCAGAA GCACATCCCA GCTTAGCCAA
    ACAGATTCTG GCAACAAGAA ATTAAAAAGT ACTATTCAGA GAAGCACAGA AACAGGCATG
    GCAGCAGAAA TGAGAAGTCG GATGGTTCGG CAACCGAGCC GGGAGTCCAC AGATGGAAGT
    ATTAACAGTT ACAGCTCAGA GGGCAATTTA ATTTTTCCAG GGGTGAGACT GGGGGTTGAT
    AGCCAATTCA GCGACTTTCT TGATGGACTA GGACCAGCAC AACTTGTAGG CAGACAGACA
    TTAGCAACTC CAGCAATGGG TGATATTCAG ATTGGAATGG TAGATAAAAA GGGGCAATTA
    GAAGTGGAAG TTATACGAGC AAGAGGTCTA ATACAAAAGC CAGGATCAAA ATCAACTCCA
    GCTCCGTATG TAAAGGTATA TCTTTTAGAA AATGGAGCGT GTGTCGCCAA GAAAAAGACA
    AGAATTGCAC GGAAAACCCT TGATCCTCTT TATCAACAGA CTTTGGTTTT TGAAGAAAGT
    CCGAATGGAA AGGTCCTTCA GGTAATTGTC TGGGGAGATT ATGGTCGAAT GGATCACAAA
    TGCTTCATGG GTGTTGCACA GATTTTATTG GAAGAACTCG ATCTATCAAG TGTGGTGATT
    GGATGGTATA AATTGTTTCC GCCATCATCG TTGGTGGATC CAACGCTGAC ACCCCTTACT
    CGCAGGGCTT CCCAATCATC TCTTGAAAGT TCTACAGGTC CTCCTTCCAT ACGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57820
    Clone ID Related Accession (Same CDS sequence) XM_031901441.1
    Accession Version XM_031901441.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGGCAGCCCA GAAAACTGGA AAGATCTAGC
    AACCAGAAAC AAAATAGGAA AAGCACTGTT TCTGAAATAG AAAGAAATCA CCAACTCGGA
    AGCCAGTCAT CAGCTGATTC ATCTTTTAGT AGTCGACGAG GAAGGCAGCT TCCACAAGTC
    CCAGTAAGAA GCAACAGTAC AGAACAAGAT TCTGGCAACA AGAAATTAAA AAGTACTATT
    CAGAGAAGCA CAGAAACAGG CATGGCAGCA GAAATGAGAA GTCGGATGGT TCGGCAACCG
    AGCCGGGAGT CCACAGATGG AAGTATTAAC AGTTACAGCT CAGAGGGCAA TTTAATTTTT
    CCAGGGGTGA GACTGGGGGT TGATAGCCAA TTCAGCGACT TTCTTGATGG ACTAGGACCA
    GCACAACTTG TAGGCAGACA GACATTAGCA ACTCCAGCAA TGGGTGATAT TCAGATTGGA
    ATGGTAGATA AAAAGGGGCA ATTAGAAGTG GAAGTTATAC GAGCAAGAGG TCTAATACAA
    AAGCCAGGAT CAAAATCAAC TCCAGCTCCG TATGTAAAGG TATATCTTTT AGAAAATGGA
    GCGTGTGTCG CCAAGAAAAA GACAAGAATT GCACGGAAAA CCCTTGATCC TCTTTATCAA
    CAGACTTTGG TTTTTGAAGA AAGTCCGAAT GGAAAGGTCC TTCAGGTAAT TGTCTGGGGA
    GATTATGGTC GAATGGATCA CAAATGCTTC ATGGGTGTTG CACAGATTTT ATTGGAAGAA
    CTCGATCTAT CAAGTGTGGT GATTGGATGG TATAAATTGT TTCCGCCATC ATCGTTGGTG
    GATCCAACGC TGACACCCCT TACTCGCAGG GCTTCCCAAT CATCTCTTGA AAGTTCTACA
    GGTCCTCCTT CCATACGATC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757301.1
    CDS364..4347
    Translation

    Target ORF information:

    RefSeq Version XM_031901441.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901441.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGGCAGCCCA GAAAACTGGA AAGATCTAGC
    AACCAGAAAC AAAATAGGAA AAGCACTGTT TCTGAAATAG AAAGAAATCA CCAACTCGGA
    AGCCAGTCAT CAGCTGATTC ATCTTTTAGT AGTCGACGAG GAAGGCAGCT TCCACAAGTC
    CCAGTAAGAA GCAACAGTAC AGAACAAGAT TCTGGCAACA AGAAATTAAA AAGTACTATT
    CAGAGAAGCA CAGAAACAGG CATGGCAGCA GAAATGAGAA GTCGGATGGT TCGGCAACCG
    AGCCGGGAGT CCACAGATGG AAGTATTAAC AGTTACAGCT CAGAGGGCAA TTTAATTTTT
    CCAGGGGTGA GACTGGGGGT TGATAGCCAA TTCAGCGACT TTCTTGATGG ACTAGGACCA
    GCACAACTTG TAGGCAGACA GACATTAGCA ACTCCAGCAA TGGGTGATAT TCAGATTGGA
    ATGGTAGATA AAAAGGGGCA ATTAGAAGTG GAAGTTATAC GAGCAAGAGG TCTAATACAA
    AAGCCAGGAT CAAAATCAAC TCCAGCTCCG TATGTAAAGG TATATCTTTT AGAAAATGGA
    GCGTGTGTCG CCAAGAAAAA GACAAGAATT GCACGGAAAA CCCTTGATCC TCTTTATCAA
    CAGACTTTGG TTTTTGAAGA AAGTCCGAAT GGAAAGGTCC TTCAGGTAAT TGTCTGGGGA
    GATTATGGTC GAATGGATCA CAAATGCTTC ATGGGTGTTG CACAGATTTT ATTGGAAGAA
    CTCGATCTAT CAAGTGTGGT GATTGGATGG TATAAATTGT TTCCGCCATC ATCGTTGGTG
    GATCCAACGC TGACACCCCT TACTCGCAGG GCTTCCCAAT CATCTCTTGA AAGTTCTACA
    GGTCCTCCTT CCATACGATC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30345
    Clone ID Related Accession (Same CDS sequence) XM_031901426.1
    Accession Version XM_031901426.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGAAATACTG TAAGGCACAG TAAAACATTA
    CCACCCAAGA TGCCTTTACT AGAGTATGGT TCTCACTGGA GACTCTACAG TGAACTGCAG
    CCCTCCCTTG ACAGGGCTAG AAGTGCTAGT ACCAACTGCT TGAGACCAGA TACTAGTTTG
    CATTCACCAG AACGAGAAAG GCAGCCCAGA AAACTGGAAA GATCTAGCAA CCAGAAACAA
    AATAGGAAAA GCACTGTTTC TGAAATAGAA AGAAATCACC AACTCGGAAG CCAGTCATCA
    GCTGATTCAT CTTTTAGTAG TCGACGAGGA AGGCAGCTTC CACAAGTCCC AGTAAGAAGC
    AACAGTACAG AACAAGAACC AGAGAGTTTT AGTTCATCAT CGAAAGCAAA TTTATCGATG
    GAAGAACGTA CAAGACAGAT GAAGATAAAA GTTCAACGGT ATAAGCAAAT AGCTGGTTCT
    AGTTCAAGTC AAGAACAGGA ACGAGAGCAA TATACTAAGT ATAACACACA CAGAGATCAG
    TACAGAAGTT GTGACAACGT CTCAGCCAAA TCGTCTGATA GTGATGTCAG TGATGTGTCA
    GCCATTTCCC GTACCAGTAG TGCCTCACGT CTCAGTAGCA CAAGCTTTAT GTCAGAGCAA
    TCTGAACGCC CCCGCAGGAG ACTATGTTCC TTTACACCCA GAATGCAAAG CAGGCGGATG
    GGTACTTCAG GGATAGCCAT AACTAAGAGC ACAAGTGTGA GTGGAGAAAT TTACAAACTG
    GAACACAATG ATGGAAGTCA GTCTGACACA GCCGTTGGGA TGGTTGGGAC CGGTGGAAAG
    AAAAGGAGAT CCAGTCTAAG TGCCAAAGTA GTTGCCATAG TATCACGAAG AAGCAGAAGC
    ACATCCCAGC TTAGCCAAAC AGATTCTGGC AACAAGAAAT TAAAAAGTAC TATTCAGAGA
    AGCACAGAAA CAGGCATGGC AGCAGAAATG AGAAGTCGGA TGGTTCGGCA ACCGAGCCGG
    GAGTCCACAG ATGGAAGTAT TAACAGTTAC AGCTCAGAGG GCAATTTAAT TTTTCCAGGG
    GTGAGACTGG GGGTTGATAG CCAATTCAGC GACTTTCTTG ATGGACTAGG ACCAGCACAA
    CTTGTAGGCA GACAGACATT AGCAACTCCA GCAATGGGTG ATATTCAGAT TGGAATGGTA
    GATAAAAAGG GGCAATTAGA AGTGGAAGTT ATACGAGCAA GAGGTCTAAT ACAAAAGCCA
    GGATCAAAAT CAACTCCAGC TCCGTATGTA AAGGTATATC TTTTAGAAAA TGGAGCGTGT
    GTCGCCAAGA AAAAGACAAG AATTGCACGG AAAACCCTTG ATCCTCTTTA TCAACAGACT
    TTGGTTTTTG AAGAAAGTCC GAATGGAAAG GTCCTTCAGG TAATTGTCTG GGGAGATTAT
    GGTCGAATGG ATCACAAATG CTTCATGGGT GTTGCACAGA TTTTATTGGA AGAACTCGAT
    CTATCAAGTG TGGTGATTGG ATGGTATAAA TTGTTTCCGC CATCATCGTT GGTGGATCCA
    ACGCTGACAC CCCTTACTCG CAGGGCTTCC CAATCATCTC TTGAAAGTTC TACAGGTCCT
    CCTTCCATAC GATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757286.1
    CDS362..5059
    Translation

    Target ORF information:

    RefSeq Version XM_031901426.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901426.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGAAATACTG TAAGGCACAG TAAAACATTA
    CCACCCAAGA TGCCTTTACT AGAGTATGGT TCTCACTGGA GACTCTACAG TGAACTGCAG
    CCCTCCCTTG ACAGGGCTAG AAGTGCTAGT ACCAACTGCT TGAGACCAGA TACTAGTTTG
    CATTCACCAG AACGAGAAAG GCAGCCCAGA AAACTGGAAA GATCTAGCAA CCAGAAACAA
    AATAGGAAAA GCACTGTTTC TGAAATAGAA AGAAATCACC AACTCGGAAG CCAGTCATCA
    GCTGATTCAT CTTTTAGTAG TCGACGAGGA AGGCAGCTTC CACAAGTCCC AGTAAGAAGC
    AACAGTACAG AACAAGAACC AGAGAGTTTT AGTTCATCAT CGAAAGCAAA TTTATCGATG
    GAAGAACGTA CAAGACAGAT GAAGATAAAA GTTCAACGGT ATAAGCAAAT AGCTGGTTCT
    AGTTCAAGTC AAGAACAGGA ACGAGAGCAA TATACTAAGT ATAACACACA CAGAGATCAG
    TACAGAAGTT GTGACAACGT CTCAGCCAAA TCGTCTGATA GTGATGTCAG TGATGTGTCA
    GCCATTTCCC GTACCAGTAG TGCCTCACGT CTCAGTAGCA CAAGCTTTAT GTCAGAGCAA
    TCTGAACGCC CCCGCAGGAG ACTATGTTCC TTTACACCCA GAATGCAAAG CAGGCGGATG
    GGTACTTCAG GGATAGCCAT AACTAAGAGC ACAAGTGTGA GTGGAGAAAT TTACAAACTG
    GAACACAATG ATGGAAGTCA GTCTGACACA GCCGTTGGGA TGGTTGGGAC CGGTGGAAAG
    AAAAGGAGAT CCAGTCTAAG TGCCAAAGTA GTTGCCATAG TATCACGAAG AAGCAGAAGC
    ACATCCCAGC TTAGCCAAAC AGATTCTGGC AACAAGAAAT TAAAAAGTAC TATTCAGAGA
    AGCACAGAAA CAGGCATGGC AGCAGAAATG AGAAGTCGGA TGGTTCGGCA ACCGAGCCGG
    GAGTCCACAG ATGGAAGTAT TAACAGTTAC AGCTCAGAGG GCAATTTAAT TTTTCCAGGG
    GTGAGACTGG GGGTTGATAG CCAATTCAGC GACTTTCTTG ATGGACTAGG ACCAGCACAA
    CTTGTAGGCA GACAGACATT AGCAACTCCA GCAATGGGTG ATATTCAGAT TGGAATGGTA
    GATAAAAAGG GGCAATTAGA AGTGGAAGTT ATACGAGCAA GAGGTCTAAT ACAAAAGCCA
    GGATCAAAAT CAACTCCAGC TCCGTATGTA AAGGTATATC TTTTAGAAAA TGGAGCGTGT
    GTCGCCAAGA AAAAGACAAG AATTGCACGG AAAACCCTTG ATCCTCTTTA TCAACAGACT
    TTGGTTTTTG AAGAAAGTCC GAATGGAAAG GTCCTTCAGG TAATTGTCTG GGGAGATTAT
    GGTCGAATGG ATCACAAATG CTTCATGGGT GTTGCACAGA TTTTATTGGA AGAACTCGAT
    CTATCAAGTG TGGTGATTGG ATGGTATAAA TTGTTTCCGC CATCATCGTT GGTGGATCCA
    ACGCTGACAC CCCTTACTCG CAGGGCTTCC CAATCATCTC TTGAAAGTTC TACAGGTCCT
    CCTTCCATAC GATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30346
    Clone ID Related Accession (Same CDS sequence) XM_031901429.1
    Accession Version XM_031901429.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GATCTCAGCG AGTCAGTGAT
    AGTGACATCT CAGATTTTGA TGTTGATGAT GGTATTGGAG TAGTTCCTCC AGGCTCAAGG
    TCTAATGCTA GAGAAAGTAG ATCTACTACA TTAACAGTGC CAGAACATCA AAGACCAACT
    CATCACCGCT CACGTTCAGT ATCTCCACAC CGTGTTGACG ATCAGGGCAG GACCCGTTCA
    CGTTTACCAA ATGTGCCATT ACAGAGAAGC CTAGATGAAA TCCATCAAAT GAGACGATCA
    CGTTCTCCAA CAAGACACCA TGATGCCTCC CGCAGTCCTG TCGATCGCAG GTCAAGGGAC
    TCAGACAGTC AGTATTTGTC TGAACAAGAA AGTGAACTCC TTATGCTTCC CCGGGCAAAA
    CGTGGACGAA GTGCAGAGTG CCTACATACC ATCAGAAATA CTGTAAGGCA CAGTAAAACA
    TTACCACCCA AGATGCCTTT ACTAGAGTAT GGTTCTCACT GGAGACTCTA CAGTGAACTG
    CAGCCCTCCC TTGACAGGGC TAGAAGTGCT AGTACCAACT GCTTGAGACC AGATACTAGT
    TTGCATTCAC CAGAACGAGA AAGGCAGCCC AGAAAACTGG AAAGATCTAG CAACCAGAAA
    CAAAATAGGA AAAGCACTGT TTCTGAAATA GAAAGAAATC ACCAACTCGG AAGCCAGTCA
    TCAGCTGATT CATCTTTTAG TAGTCGACGA GGAAGGCAGC TTCCACAAGT CCCAGTAAGA
    AGCAACAGTA CAGAACAAGA ACCAGAGAGT TTTAGTTCAT CATCGAAAGC AAATTTATCG
    ATGGAAGAAC GTACAAGACA GATGAAGATA AAAGTTCAAC GGTATAAGCA AATAGCTGGT
    TCTAGTTCAA GTCAAGAACA GGAACGAGAG CAATATACTA AGTATAACAC ACACAGAGAT
    CAGTACAGAA GTTGTGACAA CGTCTCAGCC AAATCGTCTG ATAGTGATGT CAGTGATGTG
    TCAGCCATTT CCCGTACCAG TAGTGCCTCA CGTCTCAGTA GCACAAGCTT TATGTCAGAG
    CAATCTGAAC GCCCCCGCAG GAGACTATGT TCCTTTACAC CCAGAATGCA AAGCAGGCGG
    ATGGGTACTT CAGGGATAGC CATAACTAAG AGCACAAGTG TGAGTGGAGA AATTTACAAA
    CTGGAACACA ATGATGGAAG TCAGTCTGAC ACAGCCGTTG GGATGGTTGG GACCGGTGGA
    AAGAAAAGGA GATCCAGTCT AAGTGCCAAA GTAGTTGCCA TAGTATCACG AAGAAGCAGA
    AGCACATCCC AGCTTAGCCA AACAGATTCT GGCAACAAGA AATTAAAAAG TACTATTCAG
    AGAAGCACAG AAACAGGCAT GGCAGCAGAA ATGAGAAGTC GGATGGTTCG GCAACCGAGC
    CGGGAGTCCA CAGATGGAAG TATTAACAGT TACAGCTCAG AGGGCAATTT AATTTTTCCA
    GGGGTGAGAC TGGGGGTTGA TAGCCAATTC AGCGACTTTC TTGATGGACT AGGACCAGCA
    CAACTTGTAG GCAGACAGAC ATTAGCAACT CCAGCAATGG GTGATATTCA GATTGGAATG
    GTAGATAAAA AGGGGCAATT AGAAGTGGAA GTTATACGAG CAAGAGGTCT AATACAAAAG
    CCAGGATCAA AATCAACTCC AGCTCCGTAT GTAAAGGTAT ATCTTTTAGA AAATGGAGCG
    TGTGTCGCCA AGAAAAAGAC AAGAATTGCA CGGAAAACCC TTGATCCTCT TTATCAACAG
    ACTTTGGTTT TTGAAGAAAG TCCGAATGGA AAGGTCCTTC AGGTAATTGT CTGGGGAGAT
    TATGGTCGAA TGGATCACAA ATGCTTCATG GGTGTTGCAC AGATTTTATT GGAAGAACTC
    GATCTATCAA GTGTGGTGAT TGGATGGTAT AAATTGTTTC CGCCATCATC GTTGGTGGAT
    CCAACGCTGA CACCCCTTAC TCGCAGGGCT TCCCAATCAT CTCTTGAAAG TTCTACAGGT
    CCTCCTTCCA TACGATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757289.1
    CDS362..5002
    Translation

    Target ORF information:

    RefSeq Version XM_031901429.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901429.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GATCTCAGCG AGTCAGTGAT
    AGTGACATCT CAGATTTTGA TGTTGATGAT GGTATTGGAG TAGTTCCTCC AGGCTCAAGG
    TCTAATGCTA GAGAAAGTAG ATCTACTACA TTAACAGTGC CAGAACATCA AAGACCAACT
    CATCACCGCT CACGTTCAGT ATCTCCACAC CGTGTTGACG ATCAGGGCAG GACCCGTTCA
    CGTTTACCAA ATGTGCCATT ACAGAGAAGC CTAGATGAAA TCCATCAAAT GAGACGATCA
    CGTTCTCCAA CAAGACACCA TGATGCCTCC CGCAGTCCTG TCGATCGCAG GTCAAGGGAC
    TCAGACAGTC AGTATTTGTC TGAACAAGAA AGTGAACTCC TTATGCTTCC CCGGGCAAAA
    CGTGGACGAA GTGCAGAGTG CCTACATACC ATCAGAAATA CTGTAAGGCA CAGTAAAACA
    TTACCACCCA AGATGCCTTT ACTAGAGTAT GGTTCTCACT GGAGACTCTA CAGTGAACTG
    CAGCCCTCCC TTGACAGGGC TAGAAGTGCT AGTACCAACT GCTTGAGACC AGATACTAGT
    TTGCATTCAC CAGAACGAGA AAGGCAGCCC AGAAAACTGG AAAGATCTAG CAACCAGAAA
    CAAAATAGGA AAAGCACTGT TTCTGAAATA GAAAGAAATC ACCAACTCGG AAGCCAGTCA
    TCAGCTGATT CATCTTTTAG TAGTCGACGA GGAAGGCAGC TTCCACAAGT CCCAGTAAGA
    AGCAACAGTA CAGAACAAGA ACCAGAGAGT TTTAGTTCAT CATCGAAAGC AAATTTATCG
    ATGGAAGAAC GTACAAGACA GATGAAGATA AAAGTTCAAC GGTATAAGCA AATAGCTGGT
    TCTAGTTCAA GTCAAGAACA GGAACGAGAG CAATATACTA AGTATAACAC ACACAGAGAT
    CAGTACAGAA GTTGTGACAA CGTCTCAGCC AAATCGTCTG ATAGTGATGT CAGTGATGTG
    TCAGCCATTT CCCGTACCAG TAGTGCCTCA CGTCTCAGTA GCACAAGCTT TATGTCAGAG
    CAATCTGAAC GCCCCCGCAG GAGACTATGT TCCTTTACAC CCAGAATGCA AAGCAGGCGG
    ATGGGTACTT CAGGGATAGC CATAACTAAG AGCACAAGTG TGAGTGGAGA AATTTACAAA
    CTGGAACACA ATGATGGAAG TCAGTCTGAC ACAGCCGTTG GGATGGTTGG GACCGGTGGA
    AAGAAAAGGA GATCCAGTCT AAGTGCCAAA GTAGTTGCCA TAGTATCACG AAGAAGCAGA
    AGCACATCCC AGCTTAGCCA AACAGATTCT GGCAACAAGA AATTAAAAAG TACTATTCAG
    AGAAGCACAG AAACAGGCAT GGCAGCAGAA ATGAGAAGTC GGATGGTTCG GCAACCGAGC
    CGGGAGTCCA CAGATGGAAG TATTAACAGT TACAGCTCAG AGGGCAATTT AATTTTTCCA
    GGGGTGAGAC TGGGGGTTGA TAGCCAATTC AGCGACTTTC TTGATGGACT AGGACCAGCA
    CAACTTGTAG GCAGACAGAC ATTAGCAACT CCAGCAATGG GTGATATTCA GATTGGAATG
    GTAGATAAAA AGGGGCAATT AGAAGTGGAA GTTATACGAG CAAGAGGTCT AATACAAAAG
    CCAGGATCAA AATCAACTCC AGCTCCGTAT GTAAAGGTAT ATCTTTTAGA AAATGGAGCG
    TGTGTCGCCA AGAAAAAGAC AAGAATTGCA CGGAAAACCC TTGATCCTCT TTATCAACAG
    ACTTTGGTTT TTGAAGAAAG TCCGAATGGA AAGGTCCTTC AGGTAATTGT CTGGGGAGAT
    TATGGTCGAA TGGATCACAA ATGCTTCATG GGTGTTGCAC AGATTTTATT GGAAGAACTC
    GATCTATCAA GTGTGGTGAT TGGATGGTAT AAATTGTTTC CGCCATCATC GTTGGTGGAT
    CCAACGCTGA CACCCCTTAC TCGCAGGGCT TCCCAATCAT CTCTTGAAAG TTCTACAGGT
    CCTCCTTCCA TACGATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30347
    Clone ID Related Accession (Same CDS sequence) XM_031901430.1
    Accession Version XM_031901430.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGCT CAAGGTCTAA TGCTAGAGAA
    AGTAGATCTA CTACATTAAC AGTGCCAGAA CATCAAAGAC CAACTCATCA CCGCTCACGT
    TCAGTATCTC CACACCGTGT TGACGATCAG GGCAGGACCC GTTCACGTTT ACCAAATGTG
    CCATTACAGA GAAGCCTAGA TGAAATCCAT CAAATGAGAC GATCACGTTC TCCAACAAGA
    CACCATGATG CCTCCCGCAG TCCTGTCGAT CGCAGGTCAA GGGACTCAGA CAGTCAGTAT
    TTGTCTGAAC AAGAAAGTGA ACTCCTTATG CTTCCCCGGG CAAAACGTGG ACGAAGTGCA
    GAGTGCCTAC ATACCATCAG AAATACTGTA AGGCACAGTA AAACATTACC ACCCAAGATG
    CCTTTACTAG AGTATGGTTC TCACTGGAGA CTCTACAGTG AACTGCAGCC CTCCCTTGAC
    AGGGCTAGAA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA
    CGAGAAAGGC AGCCCAGAAA ACTGGAAAGA TCTAGCAACC AGAAACAAAA TAGGAAAAGC
    ACTGTTTCTG AAATAGAAAG AAATCACCAA CTCGGAAGCC AGTCATCAGC TGATTCATCT
    TTTAGTAGTC GACGAGGAAG GCAGCTTCCA CAAGTCCCAG TAAGAAGCAA CAGTACAGAA
    CAAGAACCAG AGAGTTTTAG TTCATCATCG AAAGCAAATT TATCGATGGA AGAACGTACA
    AGACAGATGA AGATAAAAGT TCAACGGTAT AAGCAAATAG CTGGTTCTAG TTCAAGTCAA
    GAACAGGAAC GAGAGCAATA TACTAAGTAT AACACACACA GAGATCAGTA CAGAAGTTGT
    GACAACGTCT CAGCCAAATC GTCTGATAGT GATGTCAGTG ATGTGTCAGC CATTTCCCGT
    ACCAGTAGTG CCTCACGTCT CAGTAGCACA AGCTTTATGT CAGAGCAATC TGAACGCCCC
    CGCAGGAGAC TATGTTCCTT TACACCCAGA ATGCAAAGCA GGCGGATGGG TACTTCAGGG
    ATAGCCATAA CTAAGAGCAC AAGTGTGAGT GGAGAAATTT ACAAACTGGA ACACAATGAT
    GGAAGTCAGT CTGACACAGC CGTTGGGATG GTTGGGACCG GTGGAAAGAA AAGGAGATCC
    AGTCTAAGTG CCAAAGTAGT TGCCATAGTA TCACGAAGAA GCAGAAGCAC ATCCCAGCTT
    AGCCAAACAG ATTCTGGCAA CAAGAAATTA AAAAGTACTA TTCAGAGAAG CACAGAAACA
    GGCATGGCAG CAGAAATGAG AAGTCGGATG GTTCGGCAAC CGAGCCGGGA GTCCACAGAT
    GGAAGTATTA ACAGTTACAG CTCAGAGGGC AATTTAATTT TTCCAGGGGT GAGACTGGGG
    GTTGATAGCC AATTCAGCGA CTTTCTTGAT GGACTAGGAC CAGCACAACT TGTAGGCAGA
    CAGACATTAG CAACTCCAGC AATGGGTGAT ATTCAGATTG GAATGGTAGA TAAAAAGGGG
    CAATTAGAAG TGGAAGTTAT ACGAGCAAGA GGTCTAATAC AAAAGCCAGG ATCAAAATCA
    ACTCCAGCTC CGTATGTAAA GGTATATCTT TTAGAAAATG GAGCGTGTGT CGCCAAGAAA
    AAGACAAGAA TTGCACGGAA AACCCTTGAT CCTCTTTATC AACAGACTTT GGTTTTTGAA
    GAAAGTCCGA ATGGAAAGGT CCTTCAGGTA ATTGTCTGGG GAGATTATGG TCGAATGGAT
    CACAAATGCT TCATGGGTGT TGCACAGATT TTATTGGAAG AACTCGATCT ATCAAGTGTG
    GTGATTGGAT GGTATAAATT GTTTCCGCCA TCATCGTTGG TGGATCCAAC GCTGACACCC
    CTTACTCGCA GGGCTTCCCA ATCATCTCTT GAAAGTTCTA CAGGTCCTCC TTCCATACGA
    TCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757290.1
    CDS362..4987
    Translation

    Target ORF information:

    RefSeq Version XM_031901430.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901430.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGCT CAAGGTCTAA TGCTAGAGAA
    AGTAGATCTA CTACATTAAC AGTGCCAGAA CATCAAAGAC CAACTCATCA CCGCTCACGT
    TCAGTATCTC CACACCGTGT TGACGATCAG GGCAGGACCC GTTCACGTTT ACCAAATGTG
    CCATTACAGA GAAGCCTAGA TGAAATCCAT CAAATGAGAC GATCACGTTC TCCAACAAGA
    CACCATGATG CCTCCCGCAG TCCTGTCGAT CGCAGGTCAA GGGACTCAGA CAGTCAGTAT
    TTGTCTGAAC AAGAAAGTGA ACTCCTTATG CTTCCCCGGG CAAAACGTGG ACGAAGTGCA
    GAGTGCCTAC ATACCATCAG AAATACTGTA AGGCACAGTA AAACATTACC ACCCAAGATG
    CCTTTACTAG AGTATGGTTC TCACTGGAGA CTCTACAGTG AACTGCAGCC CTCCCTTGAC
    AGGGCTAGAA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA
    CGAGAAAGGC AGCCCAGAAA ACTGGAAAGA TCTAGCAACC AGAAACAAAA TAGGAAAAGC
    ACTGTTTCTG AAATAGAAAG AAATCACCAA CTCGGAAGCC AGTCATCAGC TGATTCATCT
    TTTAGTAGTC GACGAGGAAG GCAGCTTCCA CAAGTCCCAG TAAGAAGCAA CAGTACAGAA
    CAAGAACCAG AGAGTTTTAG TTCATCATCG AAAGCAAATT TATCGATGGA AGAACGTACA
    AGACAGATGA AGATAAAAGT TCAACGGTAT AAGCAAATAG CTGGTTCTAG TTCAAGTCAA
    GAACAGGAAC GAGAGCAATA TACTAAGTAT AACACACACA GAGATCAGTA CAGAAGTTGT
    GACAACGTCT CAGCCAAATC GTCTGATAGT GATGTCAGTG ATGTGTCAGC CATTTCCCGT
    ACCAGTAGTG CCTCACGTCT CAGTAGCACA AGCTTTATGT CAGAGCAATC TGAACGCCCC
    CGCAGGAGAC TATGTTCCTT TACACCCAGA ATGCAAAGCA GGCGGATGGG TACTTCAGGG
    ATAGCCATAA CTAAGAGCAC AAGTGTGAGT GGAGAAATTT ACAAACTGGA ACACAATGAT
    GGAAGTCAGT CTGACACAGC CGTTGGGATG GTTGGGACCG GTGGAAAGAA AAGGAGATCC
    AGTCTAAGTG CCAAAGTAGT TGCCATAGTA TCACGAAGAA GCAGAAGCAC ATCCCAGCTT
    AGCCAAACAG ATTCTGGCAA CAAGAAATTA AAAAGTACTA TTCAGAGAAG CACAGAAACA
    GGCATGGCAG CAGAAATGAG AAGTCGGATG GTTCGGCAAC CGAGCCGGGA GTCCACAGAT
    GGAAGTATTA ACAGTTACAG CTCAGAGGGC AATTTAATTT TTCCAGGGGT GAGACTGGGG
    GTTGATAGCC AATTCAGCGA CTTTCTTGAT GGACTAGGAC CAGCACAACT TGTAGGCAGA
    CAGACATTAG CAACTCCAGC AATGGGTGAT ATTCAGATTG GAATGGTAGA TAAAAAGGGG
    CAATTAGAAG TGGAAGTTAT ACGAGCAAGA GGTCTAATAC AAAAGCCAGG ATCAAAATCA
    ACTCCAGCTC CGTATGTAAA GGTATATCTT TTAGAAAATG GAGCGTGTGT CGCCAAGAAA
    AAGACAAGAA TTGCACGGAA AACCCTTGAT CCTCTTTATC AACAGACTTT GGTTTTTGAA
    GAAAGTCCGA ATGGAAAGGT CCTTCAGGTA ATTGTCTGGG GAGATTATGG TCGAATGGAT
    CACAAATGCT TCATGGGTGT TGCACAGATT TTATTGGAAG AACTCGATCT ATCAAGTGTG
    GTGATTGGAT GGTATAAATT GTTTCCGCCA TCATCGTTGG TGGATCCAAC GCTGACACCC
    CTTACTCGCA GGGCTTCCCA ATCATCTCTT GAAAGTTCTA CAGGTCCTCC TTCCATACGA
    TCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30348
    Clone ID Related Accession (Same CDS sequence) XM_031901431.1
    Accession Version XM_031901431.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGAAATACTG TAAGGCACAG TAAAACATTA
    CCACCCAAGA TGCCTTTACT AGAGTATGGT TCTCACTGGA GACTCTACAG TGAACTGCAG
    CCCTCCCTTG ACAGGGCTAG AAGTGCTAGT ACCAACTGCT TGAGACCAGA TACTAGTTTG
    CATTCACCAG AACGAGAAAG AAATCACCAA CTCGGAAGCC AGTCATCAGC TGATTCATCT
    TTTAGTAGTC GACGAGGAAG GCAGCTTCCA CAAGTCCCAG TAAGAAGCAA CAGTACAGAA
    CAAGAACCAG AGAGTTTTAG TTCATCATCG AAAGCAAATT TATCGATGGA AGAACGTACA
    AGACAGATGA AGATAAAAGT TCAACGGTAT AAGCAAATAG CTGGTTCTAG TTCAAGTCAA
    GAACAGGAAC GAGAGCAATA TACTAAGTAT AACACACACA GAGATCAGTA CAGAAGTTGT
    GACAACGTCT CAGCCAAATC GTCTGATAGT GATGTCAGTG ATGTGTCAGC CATTTCCCGT
    ACCAGTAGTG CCTCACGTCT CAGTAGCACA AGCTTTATGT CAGAGCAATC TGAACGCCCC
    CGCAGGAGAC TATGTTCCTT TACACCCAGA ATGCAAAGCA GGCGGATGGG TACTTCAGGG
    ATAGCCATAA CTAAGAGCAC AAGTGTGAGT GGAGAAATTT ACAAACTGGA ACACAATGAT
    GGAAGTCAGT CTGACACAGC CGTTGGGATG GTTGGGACCG GTGGAAAGAA AAGGAGATCC
    AGTCTAAGTG CCAAAGTAGT TGCCATAGTA TCACGAAGAA GCAGAAGCAC ATCCCAGCTT
    AGCCAAACAG ATTCTGGCAA CAAGAAATTA AAAAGTACTA TTCAGAGAAG CACAGAAACA
    GGCATGGCAG CAGAAATGAG AAGTCGGATG GTTCGGCAAC CGAGCCGGGA GTCCACAGAT
    GGAAGTATTA ACAGTTACAG CTCAGAGGGC AATTTAATTT TTCCAGGGGT GAGACTGGGG
    GTTGATAGCC AATTCAGCGA CTTTCTTGAT GGACTAGGAC CAGCACAACT TGTAGGCAGA
    CAGACATTAG CAACTCCAGC AATGGGTGAT ATTCAGATTG GAATGGTAGA TAAAAAGGGG
    CAATTAGAAG TGGAAGTTAT ACGAGCAAGA GGTCTAATAC AAAAGCCAGG ATCAAAATCA
    ACTCCAGCTC CGTATGTAAA GGTATATCTT TTAGAAAATG GAGCGTGTGT CGCCAAGAAA
    AAGACAAGAA TTGCACGGAA AACCCTTGAT CCTCTTTATC AACAGACTTT GGTTTTTGAA
    GAAAGTCCGA ATGGAAAGGT CCTTCAGGTA ATTGTCTGGG GAGATTATGG TCGAATGGAT
    CACAAATGCT TCATGGGTGT TGCACAGATT TTATTGGAAG AACTCGATCT ATCAAGTGTG
    GTGATTGGAT GGTATAAATT GTTTCCGCCA TCATCGTTGG TGGATCCAAC GCTGACACCC
    CTTACTCGCA GGGCTTCCCA ATCATCTCTT GAAAGTTCTA CAGGTCCTCC TTCCATACGA
    TCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757291.1
    CDS362..4987
    Translation

    Target ORF information:

    RefSeq Version XM_031901431.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901431.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGAAATACTG TAAGGCACAG TAAAACATTA
    CCACCCAAGA TGCCTTTACT AGAGTATGGT TCTCACTGGA GACTCTACAG TGAACTGCAG
    CCCTCCCTTG ACAGGGCTAG AAGTGCTAGT ACCAACTGCT TGAGACCAGA TACTAGTTTG
    CATTCACCAG AACGAGAAAG AAATCACCAA CTCGGAAGCC AGTCATCAGC TGATTCATCT
    TTTAGTAGTC GACGAGGAAG GCAGCTTCCA CAAGTCCCAG TAAGAAGCAA CAGTACAGAA
    CAAGAACCAG AGAGTTTTAG TTCATCATCG AAAGCAAATT TATCGATGGA AGAACGTACA
    AGACAGATGA AGATAAAAGT TCAACGGTAT AAGCAAATAG CTGGTTCTAG TTCAAGTCAA
    GAACAGGAAC GAGAGCAATA TACTAAGTAT AACACACACA GAGATCAGTA CAGAAGTTGT
    GACAACGTCT CAGCCAAATC GTCTGATAGT GATGTCAGTG ATGTGTCAGC CATTTCCCGT
    ACCAGTAGTG CCTCACGTCT CAGTAGCACA AGCTTTATGT CAGAGCAATC TGAACGCCCC
    CGCAGGAGAC TATGTTCCTT TACACCCAGA ATGCAAAGCA GGCGGATGGG TACTTCAGGG
    ATAGCCATAA CTAAGAGCAC AAGTGTGAGT GGAGAAATTT ACAAACTGGA ACACAATGAT
    GGAAGTCAGT CTGACACAGC CGTTGGGATG GTTGGGACCG GTGGAAAGAA AAGGAGATCC
    AGTCTAAGTG CCAAAGTAGT TGCCATAGTA TCACGAAGAA GCAGAAGCAC ATCCCAGCTT
    AGCCAAACAG ATTCTGGCAA CAAGAAATTA AAAAGTACTA TTCAGAGAAG CACAGAAACA
    GGCATGGCAG CAGAAATGAG AAGTCGGATG GTTCGGCAAC CGAGCCGGGA GTCCACAGAT
    GGAAGTATTA ACAGTTACAG CTCAGAGGGC AATTTAATTT TTCCAGGGGT GAGACTGGGG
    GTTGATAGCC AATTCAGCGA CTTTCTTGAT GGACTAGGAC CAGCACAACT TGTAGGCAGA
    CAGACATTAG CAACTCCAGC AATGGGTGAT ATTCAGATTG GAATGGTAGA TAAAAAGGGG
    CAATTAGAAG TGGAAGTTAT ACGAGCAAGA GGTCTAATAC AAAAGCCAGG ATCAAAATCA
    ACTCCAGCTC CGTATGTAAA GGTATATCTT TTAGAAAATG GAGCGTGTGT CGCCAAGAAA
    AAGACAAGAA TTGCACGGAA AACCCTTGAT CCTCTTTATC AACAGACTTT GGTTTTTGAA
    GAAAGTCCGA ATGGAAAGGT CCTTCAGGTA ATTGTCTGGG GAGATTATGG TCGAATGGAT
    CACAAATGCT TCATGGGTGT TGCACAGATT TTATTGGAAG AACTCGATCT ATCAAGTGTG
    GTGATTGGAT GGTATAAATT GTTTCCGCCA TCATCGTTGG TGGATCCAAC GCTGACACCC
    CTTACTCGCA GGGCTTCCCA ATCATCTCTT GAAAGTTCTA CAGGTCCTCC TTCCATACGA
    TCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30349
    Clone ID Related Accession (Same CDS sequence) XM_031901432.1
    Accession Version XM_031901432.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGTGAACTGC AGCCCTCCCT TGACAGGGCT
    AGAAGTGCTA GTACCAACTG CTTGAGACCA GATACTAGTT TGCATTCACC AGAACGAGAA
    AGGCAGCCCA GAAAACTGGA AAGATCTAGC AACCAGAAAC AAAATAGGAA AAGCACTGTT
    TCTGAAATAG AAAGAAATCA CCAACTCGGA AGCCAGTCAT CAGCTGATTC ATCTTTTAGT
    AGTCGACGAG GAAGGCAGCT TCCACAAGTC CCAGTAAGAA GCAACAGTAC AGAACAAGAA
    CCAGAGAGTT TTAGTTCATC ATCGAAAGCA AATTTATCGA TGGAAGAACG TACAAGACAG
    ATGAAGATAA AAGTTCAACG GTATAAGCAA ATAGCTGGTT CTAGTTCAAG TCAAGAACAG
    GAACGAGAGC AATATACTAA GTATAACACA CACAGAGATC AGTACAGAAG TTGTGACAAC
    GTCTCAGCCA AATCGTCTGA TAGTGATGTC AGTGATGTGT CAGCCATTTC CCGTACCAGT
    AGTGCCTCAC GTCTCAGTAG CACAAGCTTT ATGTCAGAGC AATCTGAACG CCCCCGCAGG
    AGACTATGTT CCTTTACACC CAGAATGCAA AGCAGGCGGA TGGGTACTTC AGGGATAGCC
    ATAACTAAGA GCACAAGTGT GAGTGGAGAA ATTTACAAAC TGGAACACAA TGATGGAAGT
    CAGTCTGACA CAGCCGTTGG GATGGTTGGG ACCGGTGGAA AGAAAAGGAG ATCCAGTCTA
    AGTGCCAAAG TAGTTGCCAT AGTATCACGA AGAAGCAGAA GCACATCCCA GCTTAGCCAA
    ACAGATTCTG GCAACAAGAA ATTAAAAAGT ACTATTCAGA GAAGCACAGA AACAGGCATG
    GCAGCAGAAA TGAGAAGTCG GATGGTTCGG CAACCGAGCC GGGAGTCCAC AGATGGAAGT
    ATTAACAGTT ACAGCTCAGA GGGCAATTTA ATTTTTCCAG GGGTGAGACT GGGGGTTGAT
    AGCCAATTCA GCGACTTTCT TGATGGACTA GGACCAGCAC AACTTGTAGG CAGACAGACA
    TTAGCAACTC CAGCAATGGG TGATATTCAG ATTGGAATGG TAGATAAAAA GGGGCAATTA
    GAAGTGGAAG TTATACGAGC AAGAGGTCTA ATACAAAAGC CAGGATCAAA ATCAACTCCA
    GCTCCGTATG TAAAGGTATA TCTTTTAGAA AATGGAGCGT GTGTCGCCAA GAAAAAGACA
    AGAATTGCAC GGAAAACCCT TGATCCTCTT TATCAACAGA CTTTGGTTTT TGAAGAAAGT
    CCGAATGGAA AGGTCCTTCA GGTAATTGTC TGGGGAGATT ATGGTCGAAT GGATCACAAA
    TGCTTCATGG GTGTTGCACA GATTTTATTG GAAGAACTCG ATCTATCAAG TGTGGTGATT
    GGATGGTATA AATTGTTTCC GCCATCATCG TTGGTGGATC CAACGCTGAC ACCCCTTACT
    CGCAGGGCTT CCCAATCATC TCTTGAAAGT TCTACAGGTC CTCCTTCCAT ACGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757292.1
    CDS362..4981
    Translation

    Target ORF information:

    RefSeq Version XM_031901432.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901432.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGTGAACTGC AGCCCTCCCT TGACAGGGCT
    AGAAGTGCTA GTACCAACTG CTTGAGACCA GATACTAGTT TGCATTCACC AGAACGAGAA
    AGGCAGCCCA GAAAACTGGA AAGATCTAGC AACCAGAAAC AAAATAGGAA AAGCACTGTT
    TCTGAAATAG AAAGAAATCA CCAACTCGGA AGCCAGTCAT CAGCTGATTC ATCTTTTAGT
    AGTCGACGAG GAAGGCAGCT TCCACAAGTC CCAGTAAGAA GCAACAGTAC AGAACAAGAA
    CCAGAGAGTT TTAGTTCATC ATCGAAAGCA AATTTATCGA TGGAAGAACG TACAAGACAG
    ATGAAGATAA AAGTTCAACG GTATAAGCAA ATAGCTGGTT CTAGTTCAAG TCAAGAACAG
    GAACGAGAGC AATATACTAA GTATAACACA CACAGAGATC AGTACAGAAG TTGTGACAAC
    GTCTCAGCCA AATCGTCTGA TAGTGATGTC AGTGATGTGT CAGCCATTTC CCGTACCAGT
    AGTGCCTCAC GTCTCAGTAG CACAAGCTTT ATGTCAGAGC AATCTGAACG CCCCCGCAGG
    AGACTATGTT CCTTTACACC CAGAATGCAA AGCAGGCGGA TGGGTACTTC AGGGATAGCC
    ATAACTAAGA GCACAAGTGT GAGTGGAGAA ATTTACAAAC TGGAACACAA TGATGGAAGT
    CAGTCTGACA CAGCCGTTGG GATGGTTGGG ACCGGTGGAA AGAAAAGGAG ATCCAGTCTA
    AGTGCCAAAG TAGTTGCCAT AGTATCACGA AGAAGCAGAA GCACATCCCA GCTTAGCCAA
    ACAGATTCTG GCAACAAGAA ATTAAAAAGT ACTATTCAGA GAAGCACAGA AACAGGCATG
    GCAGCAGAAA TGAGAAGTCG GATGGTTCGG CAACCGAGCC GGGAGTCCAC AGATGGAAGT
    ATTAACAGTT ACAGCTCAGA GGGCAATTTA ATTTTTCCAG GGGTGAGACT GGGGGTTGAT
    AGCCAATTCA GCGACTTTCT TGATGGACTA GGACCAGCAC AACTTGTAGG CAGACAGACA
    TTAGCAACTC CAGCAATGGG TGATATTCAG ATTGGAATGG TAGATAAAAA GGGGCAATTA
    GAAGTGGAAG TTATACGAGC AAGAGGTCTA ATACAAAAGC CAGGATCAAA ATCAACTCCA
    GCTCCGTATG TAAAGGTATA TCTTTTAGAA AATGGAGCGT GTGTCGCCAA GAAAAAGACA
    AGAATTGCAC GGAAAACCCT TGATCCTCTT TATCAACAGA CTTTGGTTTT TGAAGAAAGT
    CCGAATGGAA AGGTCCTTCA GGTAATTGTC TGGGGAGATT ATGGTCGAAT GGATCACAAA
    TGCTTCATGG GTGTTGCACA GATTTTATTG GAAGAACTCG ATCTATCAAG TGTGGTGATT
    GGATGGTATA AATTGTTTCC GCCATCATCG TTGGTGGATC CAACGCTGAC ACCCCTTACT
    CGCAGGGCTT CCCAATCATC TCTTGAAAGT TCTACAGGTC CTCCTTCCAT ACGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30350
    Clone ID Related Accession (Same CDS sequence) XM_031901434.1
    Accession Version XM_031901434.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GATCTCAGCG AGTCAGTGAT
    AGTGACATCT CAGATTTTGA TGTTGATGAT GGTATTGGAG TAGTTCCTCC AGGCTCAAGG
    TCTAATGCTA GAGAAAGTAG ATCTACTACA TTAACAGTGC CAGAACATCA AAGACCAACT
    CATCACCGCT CACGTTCAGT ATCTCCACAC CGTGTTGACG ATCAGGGCAG GACCCGTTCA
    CGTTTACCAA ATGTGCCATT ACAGAGAAGC CTAGATGAAA TCCATCAAAT GAGACGATCA
    CGTTCTCCAA CAAGACACCA TGATGCCTCC CGCAGTCCTG TCGATCGCAG GTCAAGGGAC
    TCAGACAGTC AGTATTTGTC TGAACAAGAA AGTGAACTCC TTATGCTTCC CCGGGCAAAA
    CGTGGACGAA GTGCAGAGTG CCTACATACC ATCAGTGAAC TGCAGCCCTC CCTTGACAGG
    GCTAGAAGTG CTAGTACCAA CTGCTTGAGA CCAGATACTA GTTTGCATTC ACCAGAACGA
    GAAAGGCAGC CCAGAAAACT GGAAAGATCT AGCAACCAGA AACAAAATAG GAAAAGCACT
    GTTTCTGAAA TAGAAAGAAA TCACCAACTC GGAAGCCAGT CATCAGCTGA TTCATCTTTT
    AGTAGTCGAC GAGGAAGGCA GCTTCCACAA GTCCCAGTAA GAAGCAACAG TACAGAACAA
    GAACCAGAGA GTTTTAGTTC ATCATCGAAA GCAAATTTAT CGATGGAAGA ACGTACAAGA
    CAGATGAAGA TAAAAGTTCA ACGGTATAAG CAAATAGCTG GTTCTAGTTC AAGTCAAGAA
    CAGGAACGAG AGCAATATAC TAAGTATAAC ACACACAGAG ATCAGTACAG AAGTTGTGAC
    AACGTCTCAG CCAAATCGTC TGATAGTGAT GTCAGTGATG TGTCAGCCAT TTCCCGTACC
    AGTAGTGCCT CACGTCTCAG TAGCACAAGC TTTATGTCAG AGCAATCTGA ACGCCCCCGC
    AGGAGACTAT GTTCCTTTAC ACCCAGAATG CAAAGCAGGC GGATGGGTAC TTCAGGGATA
    GCCATAACTA AGAGCACAAG TGTGAGTGGA GAAATTTACA AACTGGAACA CAATGATGGA
    AGTCAGTCTG ACACAGCCGT TGGGATGGTT GGGACCGGTG GAAAGAAAAG GAGATCCAGT
    CTAAGTGCCA AAGTAGTTGC CATAGTATCA CGAAGAAGCA GAAGCACATC CCAGCTTAGC
    CAAACAGATT CTGGCAACAA GAAATTAAAA AGTACTATTC AGAGAAGCAC AGAAACAGGC
    ATGGCAGCAG AAATGAGAAG TCGGATGGTT CGGCAACCGA GCCGGGAGTC CACAGATGGA
    AGTATTAACA GTTACAGCTC AGAGGGCAAT TTAATTTTTC CAGGGGTGAG ACTGGGGGTT
    GATAGCCAAT TCAGCGACTT TCTTGATGGA CTAGGACCAG CACAACTTGT AGGCAGACAG
    ACATTAGCAA CTCCAGCAAT GGGTGATATT CAGATTGGAA TGGTAGATAA AAAGGGGCAA
    TTAGAAGTGG AAGTTATACG AGCAAGAGGT CTAATACAAA AGCCAGGATC AAAATCAACT
    CCAGCTCCGT ATGTAAAGGT ATATCTTTTA GAAAATGGAG CGTGTGTCGC CAAGAAAAAG
    ACAAGAATTG CACGGAAAAC CCTTGATCCT CTTTATCAAC AGACTTTGGT TTTTGAAGAA
    AGTCCGAATG GAAAGGTCCT TCAGGTAATT GTCTGGGGAG ATTATGGTCG AATGGATCAC
    AAATGCTTCA TGGGTGTTGC ACAGATTTTA TTGGAAGAAC TCGATCTATC AAGTGTGGTG
    ATTGGATGGT ATAAATTGTT TCCGCCATCA TCGTTGGTGG ATCCAACGCT GACACCCCTT
    ACTCGCAGGG CTTCCCAATC ATCTCTTGAA AGTTCTACAG GTCCTCCTTC CATACGATCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757294.1
    CDS361..4923
    Translation

    Target ORF information:

    RefSeq Version XM_031901434.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901434.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GATCTCAGCG AGTCAGTGAT
    AGTGACATCT CAGATTTTGA TGTTGATGAT GGTATTGGAG TAGTTCCTCC AGGCTCAAGG
    TCTAATGCTA GAGAAAGTAG ATCTACTACA TTAACAGTGC CAGAACATCA AAGACCAACT
    CATCACCGCT CACGTTCAGT ATCTCCACAC CGTGTTGACG ATCAGGGCAG GACCCGTTCA
    CGTTTACCAA ATGTGCCATT ACAGAGAAGC CTAGATGAAA TCCATCAAAT GAGACGATCA
    CGTTCTCCAA CAAGACACCA TGATGCCTCC CGCAGTCCTG TCGATCGCAG GTCAAGGGAC
    TCAGACAGTC AGTATTTGTC TGAACAAGAA AGTGAACTCC TTATGCTTCC CCGGGCAAAA
    CGTGGACGAA GTGCAGAGTG CCTACATACC ATCAGTGAAC TGCAGCCCTC CCTTGACAGG
    GCTAGAAGTG CTAGTACCAA CTGCTTGAGA CCAGATACTA GTTTGCATTC ACCAGAACGA
    GAAAGGCAGC CCAGAAAACT GGAAAGATCT AGCAACCAGA AACAAAATAG GAAAAGCACT
    GTTTCTGAAA TAGAAAGAAA TCACCAACTC GGAAGCCAGT CATCAGCTGA TTCATCTTTT
    AGTAGTCGAC GAGGAAGGCA GCTTCCACAA GTCCCAGTAA GAAGCAACAG TACAGAACAA
    GAACCAGAGA GTTTTAGTTC ATCATCGAAA GCAAATTTAT CGATGGAAGA ACGTACAAGA
    CAGATGAAGA TAAAAGTTCA ACGGTATAAG CAAATAGCTG GTTCTAGTTC AAGTCAAGAA
    CAGGAACGAG AGCAATATAC TAAGTATAAC ACACACAGAG ATCAGTACAG AAGTTGTGAC
    AACGTCTCAG CCAAATCGTC TGATAGTGAT GTCAGTGATG TGTCAGCCAT TTCCCGTACC
    AGTAGTGCCT CACGTCTCAG TAGCACAAGC TTTATGTCAG AGCAATCTGA ACGCCCCCGC
    AGGAGACTAT GTTCCTTTAC ACCCAGAATG CAAAGCAGGC GGATGGGTAC TTCAGGGATA
    GCCATAACTA AGAGCACAAG TGTGAGTGGA GAAATTTACA AACTGGAACA CAATGATGGA
    AGTCAGTCTG ACACAGCCGT TGGGATGGTT GGGACCGGTG GAAAGAAAAG GAGATCCAGT
    CTAAGTGCCA AAGTAGTTGC CATAGTATCA CGAAGAAGCA GAAGCACATC CCAGCTTAGC
    CAAACAGATT CTGGCAACAA GAAATTAAAA AGTACTATTC AGAGAAGCAC AGAAACAGGC
    ATGGCAGCAG AAATGAGAAG TCGGATGGTT CGGCAACCGA GCCGGGAGTC CACAGATGGA
    AGTATTAACA GTTACAGCTC AGAGGGCAAT TTAATTTTTC CAGGGGTGAG ACTGGGGGTT
    GATAGCCAAT TCAGCGACTT TCTTGATGGA CTAGGACCAG CACAACTTGT AGGCAGACAG
    ACATTAGCAA CTCCAGCAAT GGGTGATATT CAGATTGGAA TGGTAGATAA AAAGGGGCAA
    TTAGAAGTGG AAGTTATACG AGCAAGAGGT CTAATACAAA AGCCAGGATC AAAATCAACT
    CCAGCTCCGT ATGTAAAGGT ATATCTTTTA GAAAATGGAG CGTGTGTCGC CAAGAAAAAG
    ACAAGAATTG CACGGAAAAC CCTTGATCCT CTTTATCAAC AGACTTTGGT TTTTGAAGAA
    AGTCCGAATG GAAAGGTCCT TCAGGTAATT GTCTGGGGAG ATTATGGTCG AATGGATCAC
    AAATGCTTCA TGGGTGTTGC ACAGATTTTA TTGGAAGAAC TCGATCTATC AAGTGTGGTG
    ATTGGATGGT ATAAATTGTT TCCGCCATCA TCGTTGGTGG ATCCAACGCT GACACCCCTT
    ACTCGCAGGG CTTCCCAATC ATCTCTTGAA AGTTCTACAG GTCCTCCTTC CATACGATCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44292
    Clone ID Related Accession (Same CDS sequence) XM_018093750.1
    Accession Version XM_018093750.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4569bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGAAATACT GTAAGGCACA GTAAAACATT ACCACCCAAG
    ATGCCTTTAC TAGAGTATGG TTCTCACTGG AGACTCTACA GTGAACTGCA GCCCTCCCTT
    GACAGGGCTA GAAGTGCTAG TACCAACTGC TTGAGACCAG ATACTAGTTT GCATTCACCA
    GAACGAGAAA GGCAGCCCAG AAAACTGGAA AGATCTAGCA ACCAGAAACA AAATAGGAAA
    AGCACTGTTT CTGAAATAGA AAGAAATCAC CAACTCGGAA GCCAGTCATC AGCTGATTCA
    TCTTTTAGTA GTCGACGAGG AAGGCAGCTT CCACAAGTCC CAGTAAGAAG CAACAGTACA
    GAACAAGAAC CAGAGAGTTT TAGTTCATCA TCGAAAGCAA ATTTATCGAT GGAAGAACGT
    ACAAGACAGA TGAAGATAAA AGTTCAACGG TATAAGCAAA TAGCTGGTTC TAGTTCAAGT
    CAAGAACAGG AACGAGAGCA ATATACTAAG TATAACACAC ACAGAGATCA GTACAGAAGT
    TGTGACAACG TCTCAGCCAA ATCGTCTGAT AGTGATGTCA GTGATGTGTC AGCCATTTCC
    CGTACCAGTA GTGCCTCACG TCTCAGTAGC ACAAGCTTTA TGTCAGAGCA ATCTGAACGC
    CCCCGCAGGA GACTATGTTC CTTTACACCC AGAATGCAAA GCAGGCGGAT GGGTACTTCA
    GGGATAGCCA TAACTAAGAG CACAAGTGTG AGTGGAGAAA TTTACAAACT GGAACACAAT
    GATGGAAGTC AGTCTGACAC AGCCGTTGGG ATGGTTGGGA CCGGTGGAAA GAAAAGGAGA
    TCCAGTCTAA GTGCCAAAGT AGTTGCCATA GTATCACGAA GAAGCAGAAG CACATCCCAG
    CTTAGCCAAA CAGATTCTGG CAACAAGAAA TTAAAAAGTA CTATTCAGAG AAGCACAGAA
    ACAGGCATGG CAGCAGAAAT GAGAAGTCGG ATGGTTCGGC AACCGAGCCG GGAGTCCACA
    GATGGAAGTA TTAACAGTTA CAGCTCAGAG GGCAATTTAA TTTTTCCAGG GGTGAGACTG
    GGGGTTGATA GCCAATTCAG CGACTTTCTT GATGGACTAG GACCAGCACA ACTTGTAGGC
    AGACAGACAT TAGCAACTCC AGCAATGGGT GATATTCAGA TTGGAATGGT AGATAAAAAG
    GGGCAATTAG AAGTGGAAGT TATACGAGCA AGAGGTCTAA TACAAAAGCC AGGATCAAAA
    TCAACTCCAG CTCCGTATGT AAAGGTATAT CTTTTAGAAA ATGGAGCGTG TGTCGCCAAG
    AAAAAGACAA GAATTGCACG GAAAACCCTT GATCCTCTTT ATCAACAGAC TTTGGTTTTT
    GAAGAAAGTC CGAATGGAAA GGTCCTTCAG GTAATTGTCT GGGGAGATTA TGGTCGAATG
    GATCACAAAT GCTTCATGGG TGTTGCACAG ATTTTATTGG AAGAACTCGA TCTATCAAGT
    GTGGTGATTG GATGGTATAA ATTGTTTCCG CCATCATCGT TGGTGGATCC AACGCTGACA
    CCCCTTACTC GCAGGGCTTC CCAATCATCT CTTGAAAGTT CTACAGGTCC TCCTTCCATA
    CGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949239.1
    CDS371..4939
    Translation

    Target ORF information:

    RefSeq Version XM_018093750.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GCTCAAGGTC TAATGCTAGA
    GAAAGTAGAT CTACTACATT AACAGTGCCA GAACATCAAA GACCAACTCA TCACCGCTCA
    CGTTCAGTAT CTCCACACCG TGTTGACGAT CAGGGCAGGA CCCGTTCACG TTTACCAAAT
    GTGCCATTAC AGAGAAGCCT AGATGAAATC CATCAAATGA GACGATCACG TTCTCCAACA
    AGACACCATG ATGCCTCCCG CAGTCCTGTC GATCGCAGGT CAAGGGACTC AGACAGTCAG
    TATTTGTCTG AACAAGAAAG TGAACTCCTT ATGCTTCCCC GGGCAAAACG TGGACGAAGT
    GCAGAGTGCC TACATACCAT CAGAAATACT GTAAGGCACA GTAAAACATT ACCACCCAAG
    ATGCCTTTAC TAGAGTATGG TTCTCACTGG AGACTCTACA GTGAACTGCA GCCCTCCCTT
    GACAGGGCTA GAAGTGCTAG TACCAACTGC TTGAGACCAG ATACTAGTTT GCATTCACCA
    GAACGAGAAA GGCAGCCCAG AAAACTGGAA AGATCTAGCA ACCAGAAACA AAATAGGAAA
    AGCACTGTTT CTGAAATAGA AAGAAATCAC CAACTCGGAA GCCAGTCATC AGCTGATTCA
    TCTTTTAGTA GTCGACGAGG AAGGCAGCTT CCACAAGTCC CAGTAAGAAG CAACAGTACA
    GAACAAGAAC CAGAGAGTTT TAGTTCATCA TCGAAAGCAA ATTTATCGAT GGAAGAACGT
    ACAAGACAGA TGAAGATAAA AGTTCAACGG TATAAGCAAA TAGCTGGTTC TAGTTCAAGT
    CAAGAACAGG AACGAGAGCA ATATACTAAG TATAACACAC ACAGAGATCA GTACAGAAGT
    TGTGACAACG TCTCAGCCAA ATCGTCTGAT AGTGATGTCA GTGATGTGTC AGCCATTTCC
    CGTACCAGTA GTGCCTCACG TCTCAGTAGC ACAAGCTTTA TGTCAGAGCA ATCTGAACGC
    CCCCGCAGGA GACTATGTTC CTTTACACCC AGAATGCAAA GCAGGCGGAT GGGTACTTCA
    GGGATAGCCA TAACTAAGAG CACAAGTGTG AGTGGAGAAA TTTACAAACT GGAACACAAT
    GATGGAAGTC AGTCTGACAC AGCCGTTGGG ATGGTTGGGA CCGGTGGAAA GAAAAGGAGA
    TCCAGTCTAA GTGCCAAAGT AGTTGCCATA GTATCACGAA GAAGCAGAAG CACATCCCAG
    CTTAGCCAAA CAGATTCTGG CAACAAGAAA TTAAAAAGTA CTATTCAGAG AAGCACAGAA
    ACAGGCATGG CAGCAGAAAT GAGAAGTCGG ATGGTTCGGC AACCGAGCCG GGAGTCCACA
    GATGGAAGTA TTAACAGTTA CAGCTCAGAG GGCAATTTAA TTTTTCCAGG GGTGAGACTG
    GGGGTTGATA GCCAATTCAG CGACTTTCTT GATGGACTAG GACCAGCACA ACTTGTAGGC
    AGACAGACAT TAGCAACTCC AGCAATGGGT GATATTCAGA TTGGAATGGT AGATAAAAAG
    GGGCAATTAG AAGTGGAAGT TATACGAGCA AGAGGTCTAA TACAAAAGCC AGGATCAAAA
    TCAACTCCAG CTCCGTATGT AAAGGTATAT CTTTTAGAAA ATGGAGCGTG TGTCGCCAAG
    AAAAAGACAA GAATTGCACG GAAAACCCTT GATCCTCTTT ATCAACAGAC TTTGGTTTTT
    GAAGAAAGTC CGAATGGAAA GGTCCTTCAG GTAATTGTCT GGGGAGATTA TGGTCGAATG
    GATCACAAAT GCTTCATGGG TGTTGCACAG ATTTTATTGG AAGAACTCGA TCTATCAAGT
    GTGGTGATTG GATGGTATAA ATTGTTTCCG CCATCATCGT TGGTGGATCC AACGCTGACA
    CCCCTTACTC GCAGGGCTTC CCAATCATCT CTTGAAAGTT CTACAGGTCC TCCTTCCATA
    CGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57814
    Clone ID Related Accession (Same CDS sequence) XM_031901427.1
    Accession Version XM_031901427.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGT TATGTGGGTT TGCAATTTGT GTCGGAAGCA ACAGGAAATT
    TTGACAAAAT CTGGGGACTG GTTCTTTGGA AGTGGGGTGC AGCAGCCATC TTCTCAGGAT
    GGAACATTAA GTGACACTGC TACAGGAGCA AGTACGGATG CACCAAGGGA AAAGAAAGCT
    AGACTTCAAG AGAGATCTCG ATCTCAAACG CCTCTAAGCA CCGCTGCAGT GGCAGGTTCA
    CAAGGAACCC CTCCTTCTAG TTTACAGTCT GATAGGAGCA AAGGCTCAGA ACCATCACTA
    CAATCCTCAG TCCAAGAGCC AAAATTATCA TCAAGATCAA GAAGTGAACC TCCAAAAGAC
    AGGAAGAAGG CCTTAGCAAT TTCAGAGCAA AATGGAAAAG TCTTGAAAAG TGATCGAAAA
    AGAGTTCAGA AGTGTTTAAA CCAACAAGCT GAGGGACATA TGGATGACAG AGAACATAAA
    GAAAGGAGAG AAAGCAGAAG ATTAGGGAAA GGAAGGTCCC AGGACATTCC AGAAAAAGCA
    GAGGAAGATA CAAACATGGA AGAAGACAAA CAAAGAACAG AGGATGAGTA TCAAACACGA
    TACAGAAGTG ATCCTAATTT AGCCAGGTAT CCAGTAAAAC CACAACCAGA AGAACAGCAA
    ATGCGCATGC ATGCAAAGGT TTCAAAGGCA AGGCATGAAC GACGGCACAG TGATGCATCT
    TTACCACATA CAGAGCTAGA TGAAGCTGAA GTTCCTGAGA ATAAACTAGG CAAAAACTCA
    CAAGGATCAG CATCTCAGGA CAAAAAATCA GTTTTGGAGA ATAAGAGGTC TTATTCTGTA
    GACAGAAATG GTGAAGTATT AGTTTCCAAG CCAAAACAAT CTGCCAATCA CAGTCTCCAT
    AGTTCTCAAC ACAGTCCGGT TCCTCCAGAA CATGTAGACT CTATGAACCA TGATTCCTTT
    AAAAAACAGA GTAGATTAGA CCCAAGTTCT GCTCTTTTAA CAAGGAAAGC TACACGTGAA
    AAGATGGAGA CAATGTTGAG AAATGACTCC CTTAGTTCCG ATCAATCAGA ATCTGTTAGA
    CCTTCCCCAC CAAAGCCCCA CAGGTCGAAA AGAGGAGGGA AAAAAAGACA AATGTCAGTT
    AGTAGCTCAG ATGATGAAGG AGCATCAACC CCGGAATATA CAAGCTGTGA AGATGCAGAA
    ATAGAAAGTG AAAGTGTTAG TGAAAAAGCG GCTCTCTTTG GCAGAAGACA GTTGGAGTGC
    CCTGTCTCCT CTCAGTCGTG CTGTGGTGAT TTGGACTGTT TTTGGTTGGA TCCTGCTACA
    TGGCATAGCA GAGAAACTTC ACCCCTAGGG TCGCATCCAG TAACCTGGCA GCCATCTAAG
    GAGGGGGATC GTTTAATTGG TCGCGTAATT CTAAATAAAA GAACAAGTAT GCCAAAAGAA
    TCTGGATCAC TTCTGGGTTT GAAAGTTGTA GGGGGAAAAT TGTCAGAAAC AGGACGCCTT
    GGTGCCTTCA TCACCAAAGT AAAGAAAGGG AGCCTCGCAG ATGTTGTTGG CCATTTAAGA
    GCAGGAGATG AAGTCTTAGA ATGGAATGGA AAACCTTTGC CAGGAGCAAC AAATGAAGAT
    GTTTACAACA TTATTTTACA ATCAAAGTCA GAGGCACAAG TTGAAATCAT AGTTTCAAGG
    CCTATTGGAG ACATTCCAAG GATTCCAGAA AACTCTCATC CACCATTAGA ATCTAGTTCC
    AGTTCTTTTG AATCTCAAAA GATGGATCGG CCTTCAATAT CTGTCATTTC TCCAACTAGT
    CCTGGAGCTC TAAAAGATGC ACCTCAGGTC CTGCCAGGAC AGCTTTCTGT AAAATTGTGG
    TATGACAAAG TGGGCCATCA GCTAATAGTA AATGTCTTGC AAGCAAGAGA CCTGCCAACA
    CGGCCAGATG GAAGGCCTAG GAACCCTTAT GTAAAAATGT ACTTCCTTCC GGACAGAAGC
    GATAAAAGTA AAAGGAGGAC CAAAACAGTG AAGAAAAGCT CTGAACCAAA ATGGAACCAA
    TCATTTCTCT ATTCCCATGT GCACCGTAGA GACTTTAGAG AGCGAATGTT AGAAATAACT
    GTGTGGGATC AGCCAAGAGT ACAAGAAGAG GAAAGCGAGT TTCTTGGCGA GATCCTAATA
    GAACTAGAGA CTGCACTATT AGATGATGAA CCTCACTGGT ATAAGCTACA GACGCACGAT
    GAATCTTCAC TACCTCTGCC TCAGCCATCA CCTTTCATGC CAAGGAGACA TGTTCATGGA
    GAAAATTCTA GCAAAAAATT ACAAAGATCT CAGCGAGTCA GTGATAGTGA CATCTCAGAT
    TTTGATGTTG ATGATGGTAT TGGAGTAGTT CCTCCAGGCT CAAGGTCTAA TGCTAGAGAA
    AGTAGATCTA CTACATTAAC AGTGCCAGAA CATCAAAGAC CAACTCATCA CCGCTCACGT
    TCAGTATCTC CACACCGTGT TGACGATCAG GGCAGGACCC GTTCACGTTT ACCAAATGTG
    CCATTACAGA GAAGCCTAGA TGAAATCCAT CAAATGAGAC GATCACGTTC TCCAACAAGA
    CACCATGATG CCTCCCGCAG TCCTGTCGAT CGCAGGTCAA GGGACTCAGA CAGTCAGTAT
    TTGTCTGAAC AAGAAAGTGA ACTCCTTATG CTTCCCCGGG CAAAACGTGG ACGAAGTGCA
    GAGTGCCTAC ATACCATCAG AAATACTGTA AGGCACAGTA AAACATTACC ACCCAAGATG
    CCTTTACTAG AGTATGGTTC TCACTGGAGA CTCTACAGTG AACTGCAGCC CTCCCTTGAC
    AGGGCTAGAA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA
    CGAGAAAGGC AGCCCAGAAA ACTGGAAAGA TCTAGCAACC AGAAACAAAA TAGGAAAAGC
    ACTGTTTCTG AAATAGAAAG AAATCACCAA CTCGGAAGCC AGTCATCAGC TGATTCATCT
    TTTAGTAGTC GACGAGGAAG GCAGCTTCCA CAAGTCCCAG TAAGAAGCAA CAGTACAGAA
    CAAGAACCAG AGAGTTTTAG TTCATCATCG AAAGCAAATT TATCGATGGA AGAACGTACA
    AGACAGATGA AGATAAAAGT TCAACGGTAT AAGCAAATAG CTGGTTCTAG TTCAAGTCAA
    GAACAGGAAC GAGAGCAATA TACTAAGTAT AACACACACA GAGATCAGTA CAGAAGTTGT
    GACAACGTCT CAGCCAAATC GTCTGATAGT GATGTCAGTG ATGTGTCAGC CATTTCCCGT
    ACCAGTAGTG CCTCACGTCT CAGTAGCACA AGCTTTATGT CAGAGCAATC TGAACGCCCC
    CGCAGGAGAC TATGTTCCTT TACACCCAGA ATGCAAAGCA GGCGGATGGG TACTTCAGGG
    ATAGCCATAA CTAAGAGCAC AAGTGTGAGT GGAGAAATTT ACAAACTGGA ACACAATGAT
    GGAAGTCAGT CTGACACAGC CGTTGGGATG GTTGGGACCG GTGGAAAGAA AAGGAGATCC
    AGTCTAAGTG CCAAAGTAGT TGCCATAGTA TCACGAAGAA GCAGAAGCAC ATCCCAGCTT
    AGCCAAACAG ATTCTGGCAA CAAGAAATTA AAAAGTACTA TTCAGAGAAG CACAGAAACA
    GGCATGGCAG CAGAAATGAG AAGTCGGATG GTTCGGCAAC CGAGCCGGGA GTCCACAGAT
    GGAAGTATTA ACAGTTACAG CTCAGAGGGC AATTTAATTT TTCCAGGGGT GAGACTGGGG
    GTTGATAGCC AATTCAGCGA CTTTCTTGAT GGACTAGGAC CAGCACAACT TGTAGGCAGA
    CAGACATTAG CAACTCCAGC AATGGGTGAT ATTCAGATTG GAATGGTAGA TAAAAAGGGG
    CAATTAGAAG TGGAAGTTAT ACGAGCAAGA GGTCTAATAC AAAAGCCAGG ATCAAAATCA
    ACTCCAGCTC CGTATGTAAA GGTATATCTT TTAGAAAATG GAGCGTGTGT CGCCAAGAAA
    AAGACAAGAA TTGCACGGAA AACCCTTGAT CCTCTTTATC AACAGACTTT GGTTTTTGAA
    GAAAGTCCGA ATGGAAAGGT CCTTCAGGTA ATTGTCTGGG GAGATTATGG TCGAATGGAT
    CACAAATGCT TCATGGGTGT TGCACAGATT TTATTGGAAG AACTCGATCT ATCAAGTGTG
    GTGATTGGAT GGTATAAATT GTTTCCGCCA TCATCGTTGG TGGATCCAAC GCTGACACCC
    CTTACTCGCA GGGCTTCCCA ATCATCTCTT GAAAGTTCTA CAGGTCCTCC TTCCATACGA
    TCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757287.1
    CDS357..5042
    Translation

    Target ORF information:

    RefSeq Version XM_031901427.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901427.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGT TATGTGGGTT TGCAATTTGT GTCGGAAGCA ACAGGAAATT
    TTGACAAAAT CTGGGGACTG GTTCTTTGGA AGTGGGGTGC AGCAGCCATC TTCTCAGGAT
    GGAACATTAA GTGACACTGC TACAGGAGCA AGTACGGATG CACCAAGGGA AAAGAAAGCT
    AGACTTCAAG AGAGATCTCG ATCTCAAACG CCTCTAAGCA CCGCTGCAGT GGCAGGTTCA
    CAAGGAACCC CTCCTTCTAG TTTACAGTCT GATAGGAGCA AAGGCTCAGA ACCATCACTA
    CAATCCTCAG TCCAAGAGCC AAAATTATCA TCAAGATCAA GAAGTGAACC TCCAAAAGAC
    AGGAAGAAGG CCTTAGCAAT TTCAGAGCAA AATGGAAAAG TCTTGAAAAG TGATCGAAAA
    AGAGTTCAGA AGTGTTTAAA CCAACAAGCT GAGGGACATA TGGATGACAG AGAACATAAA
    GAAAGGAGAG AAAGCAGAAG ATTAGGGAAA GGAAGGTCCC AGGACATTCC AGAAAAAGCA
    GAGGAAGATA CAAACATGGA AGAAGACAAA CAAAGAACAG AGGATGAGTA TCAAACACGA
    TACAGAAGTG ATCCTAATTT AGCCAGGTAT CCAGTAAAAC CACAACCAGA AGAACAGCAA
    ATGCGCATGC ATGCAAAGGT TTCAAAGGCA AGGCATGAAC GACGGCACAG TGATGCATCT
    TTACCACATA CAGAGCTAGA TGAAGCTGAA GTTCCTGAGA ATAAACTAGG CAAAAACTCA
    CAAGGATCAG CATCTCAGGA CAAAAAATCA GTTTTGGAGA ATAAGAGGTC TTATTCTGTA
    GACAGAAATG GTGAAGTATT AGTTTCCAAG CCAAAACAAT CTGCCAATCA CAGTCTCCAT
    AGTTCTCAAC ACAGTCCGGT TCCTCCAGAA CATGTAGACT CTATGAACCA TGATTCCTTT
    AAAAAACAGA GTAGATTAGA CCCAAGTTCT GCTCTTTTAA CAAGGAAAGC TACACGTGAA
    AAGATGGAGA CAATGTTGAG AAATGACTCC CTTAGTTCCG ATCAATCAGA ATCTGTTAGA
    CCTTCCCCAC CAAAGCCCCA CAGGTCGAAA AGAGGAGGGA AAAAAAGACA AATGTCAGTT
    AGTAGCTCAG ATGATGAAGG AGCATCAACC CCGGAATATA CAAGCTGTGA AGATGCAGAA
    ATAGAAAGTG AAAGTGTTAG TGAAAAAGCG GCTCTCTTTG GCAGAAGACA GTTGGAGTGC
    CCTGTCTCCT CTCAGTCGTG CTGTGGTGAT TTGGACTGTT TTTGGTTGGA TCCTGCTACA
    TGGCATAGCA GAGAAACTTC ACCCCTAGGG TCGCATCCAG TAACCTGGCA GCCATCTAAG
    GAGGGGGATC GTTTAATTGG TCGCGTAATT CTAAATAAAA GAACAAGTAT GCCAAAAGAA
    TCTGGATCAC TTCTGGGTTT GAAAGTTGTA GGGGGAAAAT TGTCAGAAAC AGGACGCCTT
    GGTGCCTTCA TCACCAAAGT AAAGAAAGGG AGCCTCGCAG ATGTTGTTGG CCATTTAAGA
    GCAGGAGATG AAGTCTTAGA ATGGAATGGA AAACCTTTGC CAGGAGCAAC AAATGAAGAT
    GTTTACAACA TTATTTTACA ATCAAAGTCA GAGGCACAAG TTGAAATCAT AGTTTCAAGG
    CCTATTGGAG ACATTCCAAG GATTCCAGAA AACTCTCATC CACCATTAGA ATCTAGTTCC
    AGTTCTTTTG AATCTCAAAA GATGGATCGG CCTTCAATAT CTGTCATTTC TCCAACTAGT
    CCTGGAGCTC TAAAAGATGC ACCTCAGGTC CTGCCAGGAC AGCTTTCTGT AAAATTGTGG
    TATGACAAAG TGGGCCATCA GCTAATAGTA AATGTCTTGC AAGCAAGAGA CCTGCCAACA
    CGGCCAGATG GAAGGCCTAG GAACCCTTAT GTAAAAATGT ACTTCCTTCC GGACAGAAGC
    GATAAAAGTA AAAGGAGGAC CAAAACAGTG AAGAAAAGCT CTGAACCAAA ATGGAACCAA
    TCATTTCTCT ATTCCCATGT GCACCGTAGA GACTTTAGAG AGCGAATGTT AGAAATAACT
    GTGTGGGATC AGCCAAGAGT ACAAGAAGAG GAAAGCGAGT TTCTTGGCGA GATCCTAATA
    GAACTAGAGA CTGCACTATT AGATGATGAA CCTCACTGGT ATAAGCTACA GACGCACGAT
    GAATCTTCAC TACCTCTGCC TCAGCCATCA CCTTTCATGC CAAGGAGACA TGTTCATGGA
    GAAAATTCTA GCAAAAAATT ACAAAGATCT CAGCGAGTCA GTGATAGTGA CATCTCAGAT
    TTTGATGTTG ATGATGGTAT TGGAGTAGTT CCTCCAGGCT CAAGGTCTAA TGCTAGAGAA
    AGTAGATCTA CTACATTAAC AGTGCCAGAA CATCAAAGAC CAACTCATCA CCGCTCACGT
    TCAGTATCTC CACACCGTGT TGACGATCAG GGCAGGACCC GTTCACGTTT ACCAAATGTG
    CCATTACAGA GAAGCCTAGA TGAAATCCAT CAAATGAGAC GATCACGTTC TCCAACAAGA
    CACCATGATG CCTCCCGCAG TCCTGTCGAT CGCAGGTCAA GGGACTCAGA CAGTCAGTAT
    TTGTCTGAAC AAGAAAGTGA ACTCCTTATG CTTCCCCGGG CAAAACGTGG ACGAAGTGCA
    GAGTGCCTAC ATACCATCAG AAATACTGTA AGGCACAGTA AAACATTACC ACCCAAGATG
    CCTTTACTAG AGTATGGTTC TCACTGGAGA CTCTACAGTG AACTGCAGCC CTCCCTTGAC
    AGGGCTAGAA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA
    CGAGAAAGGC AGCCCAGAAA ACTGGAAAGA TCTAGCAACC AGAAACAAAA TAGGAAAAGC
    ACTGTTTCTG AAATAGAAAG AAATCACCAA CTCGGAAGCC AGTCATCAGC TGATTCATCT
    TTTAGTAGTC GACGAGGAAG GCAGCTTCCA CAAGTCCCAG TAAGAAGCAA CAGTACAGAA
    CAAGAACCAG AGAGTTTTAG TTCATCATCG AAAGCAAATT TATCGATGGA AGAACGTACA
    AGACAGATGA AGATAAAAGT TCAACGGTAT AAGCAAATAG CTGGTTCTAG TTCAAGTCAA
    GAACAGGAAC GAGAGCAATA TACTAAGTAT AACACACACA GAGATCAGTA CAGAAGTTGT
    GACAACGTCT CAGCCAAATC GTCTGATAGT GATGTCAGTG ATGTGTCAGC CATTTCCCGT
    ACCAGTAGTG CCTCACGTCT CAGTAGCACA AGCTTTATGT CAGAGCAATC TGAACGCCCC
    CGCAGGAGAC TATGTTCCTT TACACCCAGA ATGCAAAGCA GGCGGATGGG TACTTCAGGG
    ATAGCCATAA CTAAGAGCAC AAGTGTGAGT GGAGAAATTT ACAAACTGGA ACACAATGAT
    GGAAGTCAGT CTGACACAGC CGTTGGGATG GTTGGGACCG GTGGAAAGAA AAGGAGATCC
    AGTCTAAGTG CCAAAGTAGT TGCCATAGTA TCACGAAGAA GCAGAAGCAC ATCCCAGCTT
    AGCCAAACAG ATTCTGGCAA CAAGAAATTA AAAAGTACTA TTCAGAGAAG CACAGAAACA
    GGCATGGCAG CAGAAATGAG AAGTCGGATG GTTCGGCAAC CGAGCCGGGA GTCCACAGAT
    GGAAGTATTA ACAGTTACAG CTCAGAGGGC AATTTAATTT TTCCAGGGGT GAGACTGGGG
    GTTGATAGCC AATTCAGCGA CTTTCTTGAT GGACTAGGAC CAGCACAACT TGTAGGCAGA
    CAGACATTAG CAACTCCAGC AATGGGTGAT ATTCAGATTG GAATGGTAGA TAAAAAGGGG
    CAATTAGAAG TGGAAGTTAT ACGAGCAAGA GGTCTAATAC AAAAGCCAGG ATCAAAATCA
    ACTCCAGCTC CGTATGTAAA GGTATATCTT TTAGAAAATG GAGCGTGTGT CGCCAAGAAA
    AAGACAAGAA TTGCACGGAA AACCCTTGAT CCTCTTTATC AACAGACTTT GGTTTTTGAA
    GAAAGTCCGA ATGGAAAGGT CCTTCAGGTA ATTGTCTGGG GAGATTATGG TCGAATGGAT
    CACAAATGCT TCATGGGTGT TGCACAGATT TTATTGGAAG AACTCGATCT ATCAAGTGTG
    GTGATTGGAT GGTATAAATT GTTTCCGCCA TCATCGTTGG TGGATCCAAC GCTGACACCC
    CTTACTCGCA GGGCTTCCCA ATCATCTCTT GAAAGTTCTA CAGGTCCTCC TTCCATACGA
    TCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57815
    Clone ID Related Accession (Same CDS sequence) XM_031901428.1
    Accession Version XM_031901428.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGAAATACTG TAAGGCACAG TAAAACATTA
    CCACCCAAGA TGCCTTTACT AGAGTATGGT TCTCACTGGA GACTCTACAG TGAACTGCAG
    CCCTCCCTTG ACAGGGCTAG AAGTGCTAGT ACCAACTGCT TGAGACCAGA TACTAGTTTG
    CATTCACCAG AACGAGAAAG GCAGCCCAGA AAACTGGAAA GATCTAGCAA CCAGAAACAA
    AATAGGAAAA GCACTGTTTC TGAAATAGAA AGAAATCACC AACTCGGAAG CCAGTCATCA
    GCTGATTCAT CTTTTAGTAG TCGACGAGGA AGGCAGCTTC CACAAGTCCC AGTAAGAAGC
    AACAGTACAG AACAAGCAAA TTTATCGATG GAAGAACGTA CAAGACAGAT GAAGATAAAA
    GTTCAACGGT ATAAGCAAAT AGCTGGTTCT AGTTCAAGTC AAGAACAGGA ACGAGAGCAA
    TATACTAAGT ATAACACACA CAGAGATCAG TACAGAAGTT GTGACAACGT CTCAGCCAAA
    TCGTCTGATA GTGATGTCAG TGATGTGTCA GCCATTTCCC GTACCAGTAG TGCCTCACGT
    CTCAGTAGCA CAAGCTTTAT GTCAGAGCAA TCTGAACGCC CCCGCAGGAG ACTATGTTCC
    TTTACACCCA GAATGCAAAG CAGGCGGATG GGTACTTCAG GGATAGCCAT AACTAAGAGC
    ACAAGTGTGA GTGGAGAAAT TTACAAACTG GAACACAATG ATGGAAGTCA GTCTGACACA
    GCCGTTGGGA TGGTTGGGAC CGGTGGAAAG AAAAGGAGAT CCAGTCTAAG TGCCAAAGTA
    GTTGCCATAG TATCACGAAG AAGCAGAAGC ACATCCCAGC TTAGCCAAAC AGATTCTGGC
    AACAAGAAAT TAAAAAGTAC TATTCAGAGA AGCACAGAAA CAGGCATGGC AGCAGAAATG
    AGAAGTCGGA TGGTTCGGCA ACCGAGCCGG GAGTCCACAG ATGGAAGTAT TAACAGTTAC
    AGCTCAGAGG GCAATTTAAT TTTTCCAGGG GTGAGACTGG GGGTTGATAG CCAATTCAGC
    GACTTTCTTG ATGGACTAGG ACCAGCACAA CTTGTAGGCA GACAGACATT AGCAACTCCA
    GCAATGGGTG ATATTCAGAT TGGAATGGTA GATAAAAAGG GGCAATTAGA AGTGGAAGTT
    ATACGAGCAA GAGGTCTAAT ACAAAAGCCA GGATCAAAAT CAACTCCAGC TCCGTATGTA
    AAGGTATATC TTTTAGAAAA TGGAGCGTGT GTCGCCAAGA AAAAGACAAG AATTGCACGG
    AAAACCCTTG ATCCTCTTTA TCAACAGACT TTGGTTTTTG AAGAAAGTCC GAATGGAAAG
    GTCCTTCAGG TAATTGTCTG GGGAGATTAT GGTCGAATGG ATCACAAATG CTTCATGGGT
    GTTGCACAGA TTTTATTGGA AGAACTCGAT CTATCAAGTG TGGTGATTGG ATGGTATAAA
    TTGTTTCCGC CATCATCGTT GGTGGATCCA ACGCTGACAC CCCTTACTCG CAGGGCTTCC
    CAATCATCTC TTGAAAGTTC TACAGGTCCT CCTTCCATAC GATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757288.1
    CDS362..5029
    Translation

    Target ORF information:

    RefSeq Version XM_031901428.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901428.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGAAATACTG TAAGGCACAG TAAAACATTA
    CCACCCAAGA TGCCTTTACT AGAGTATGGT TCTCACTGGA GACTCTACAG TGAACTGCAG
    CCCTCCCTTG ACAGGGCTAG AAGTGCTAGT ACCAACTGCT TGAGACCAGA TACTAGTTTG
    CATTCACCAG AACGAGAAAG GCAGCCCAGA AAACTGGAAA GATCTAGCAA CCAGAAACAA
    AATAGGAAAA GCACTGTTTC TGAAATAGAA AGAAATCACC AACTCGGAAG CCAGTCATCA
    GCTGATTCAT CTTTTAGTAG TCGACGAGGA AGGCAGCTTC CACAAGTCCC AGTAAGAAGC
    AACAGTACAG AACAAGCAAA TTTATCGATG GAAGAACGTA CAAGACAGAT GAAGATAAAA
    GTTCAACGGT ATAAGCAAAT AGCTGGTTCT AGTTCAAGTC AAGAACAGGA ACGAGAGCAA
    TATACTAAGT ATAACACACA CAGAGATCAG TACAGAAGTT GTGACAACGT CTCAGCCAAA
    TCGTCTGATA GTGATGTCAG TGATGTGTCA GCCATTTCCC GTACCAGTAG TGCCTCACGT
    CTCAGTAGCA CAAGCTTTAT GTCAGAGCAA TCTGAACGCC CCCGCAGGAG ACTATGTTCC
    TTTACACCCA GAATGCAAAG CAGGCGGATG GGTACTTCAG GGATAGCCAT AACTAAGAGC
    ACAAGTGTGA GTGGAGAAAT TTACAAACTG GAACACAATG ATGGAAGTCA GTCTGACACA
    GCCGTTGGGA TGGTTGGGAC CGGTGGAAAG AAAAGGAGAT CCAGTCTAAG TGCCAAAGTA
    GTTGCCATAG TATCACGAAG AAGCAGAAGC ACATCCCAGC TTAGCCAAAC AGATTCTGGC
    AACAAGAAAT TAAAAAGTAC TATTCAGAGA AGCACAGAAA CAGGCATGGC AGCAGAAATG
    AGAAGTCGGA TGGTTCGGCA ACCGAGCCGG GAGTCCACAG ATGGAAGTAT TAACAGTTAC
    AGCTCAGAGG GCAATTTAAT TTTTCCAGGG GTGAGACTGG GGGTTGATAG CCAATTCAGC
    GACTTTCTTG ATGGACTAGG ACCAGCACAA CTTGTAGGCA GACAGACATT AGCAACTCCA
    GCAATGGGTG ATATTCAGAT TGGAATGGTA GATAAAAAGG GGCAATTAGA AGTGGAAGTT
    ATACGAGCAA GAGGTCTAAT ACAAAAGCCA GGATCAAAAT CAACTCCAGC TCCGTATGTA
    AAGGTATATC TTTTAGAAAA TGGAGCGTGT GTCGCCAAGA AAAAGACAAG AATTGCACGG
    AAAACCCTTG ATCCTCTTTA TCAACAGACT TTGGTTTTTG AAGAAAGTCC GAATGGAAAG
    GTCCTTCAGG TAATTGTCTG GGGAGATTAT GGTCGAATGG ATCACAAATG CTTCATGGGT
    GTTGCACAGA TTTTATTGGA AGAACTCGAT CTATCAAGTG TGGTGATTGG ATGGTATAAA
    TTGTTTCCGC CATCATCGTT GGTGGATCCA ACGCTGACAC CCCTTACTCG CAGGGCTTCC
    CAATCATCTC TTGAAAGTTC TACAGGTCCT CCTTCCATAC GATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57816
    Clone ID Related Accession (Same CDS sequence) XM_031901433.1
    Accession Version XM_031901433.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGTGAACTGC AGCCCTCCCT TGACAGGGCT
    AGAAGTGCTA GTACCAACTG CTTGAGACCA GATACTAGTT TGCATTCACC AGAACGAGAA
    AGGCAGCCCA GAAAACTGGA AAGATCTAGC AACCAGAAAC AAAATAGGAA AAGCACTGTT
    TCTGAAATAG AAAGAAATCA CCAACTCGGA AGCCAGTCAT CAGCTGATTC ATCTTTTAGT
    AGTCGACGAG GAAGGCAGCT TCCACAAGTC CCAGTAAGAA GCAACAGTAC AGAACAAGCA
    AATTTATCGA TGGAAGAACG TACAAGACAG ATGAAGATAA AAGTTCAACG GTATAAGCAA
    ATAGCTGGTT CTAGTTCAAG TCAAGAACAG GAACGAGAGC AATATACTAA GTATAACACA
    CACAGAGATC AGTACAGAAG TTGTGACAAC GTCTCAGCCA AATCGTCTGA TAGTGATGTC
    AGTGATGTGT CAGCCATTTC CCGTACCAGT AGTGCCTCAC GTCTCAGTAG CACAAGCTTT
    ATGTCAGAGC AATCTGAACG CCCCCGCAGG AGACTATGTT CCTTTACACC CAGAATGCAA
    AGCAGGCGGA TGGGTACTTC AGGGATAGCC ATAACTAAGA GCACAAGTGT GAGTGGAGAA
    ATTTACAAAC TGGAACACAA TGATGGAAGT CAGTCTGACA CAGCCGTTGG GATGGTTGGG
    ACCGGTGGAA AGAAAAGGAG ATCCAGTCTA AGTGCCAAAG TAGTTGCCAT AGTATCACGA
    AGAAGCAGAA GCACATCCCA GCTTAGCCAA ACAGATTCTG GCAACAAGAA ATTAAAAAGT
    ACTATTCAGA GAAGCACAGA AACAGGCATG GCAGCAGAAA TGAGAAGTCG GATGGTTCGG
    CAACCGAGCC GGGAGTCCAC AGATGGAAGT ATTAACAGTT ACAGCTCAGA GGGCAATTTA
    ATTTTTCCAG GGGTGAGACT GGGGGTTGAT AGCCAATTCA GCGACTTTCT TGATGGACTA
    GGACCAGCAC AACTTGTAGG CAGACAGACA TTAGCAACTC CAGCAATGGG TGATATTCAG
    ATTGGAATGG TAGATAAAAA GGGGCAATTA GAAGTGGAAG TTATACGAGC AAGAGGTCTA
    ATACAAAAGC CAGGATCAAA ATCAACTCCA GCTCCGTATG TAAAGGTATA TCTTTTAGAA
    AATGGAGCGT GTGTCGCCAA GAAAAAGACA AGAATTGCAC GGAAAACCCT TGATCCTCTT
    TATCAACAGA CTTTGGTTTT TGAAGAAAGT CCGAATGGAA AGGTCCTTCA GGTAATTGTC
    TGGGGAGATT ATGGTCGAAT GGATCACAAA TGCTTCATGG GTGTTGCACA GATTTTATTG
    GAAGAACTCG ATCTATCAAG TGTGGTGATT GGATGGTATA AATTGTTTCC GCCATCATCG
    TTGGTGGATC CAACGCTGAC ACCCCTTACT CGCAGGGCTT CCCAATCATC TCTTGAAAGT
    TCTACAGGTC CTCCTTCCAT ACGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757293.1
    CDS362..4951
    Translation

    Target ORF information:

    RefSeq Version XM_031901433.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901433.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGTGAACTGC AGCCCTCCCT TGACAGGGCT
    AGAAGTGCTA GTACCAACTG CTTGAGACCA GATACTAGTT TGCATTCACC AGAACGAGAA
    AGGCAGCCCA GAAAACTGGA AAGATCTAGC AACCAGAAAC AAAATAGGAA AAGCACTGTT
    TCTGAAATAG AAAGAAATCA CCAACTCGGA AGCCAGTCAT CAGCTGATTC ATCTTTTAGT
    AGTCGACGAG GAAGGCAGCT TCCACAAGTC CCAGTAAGAA GCAACAGTAC AGAACAAGCA
    AATTTATCGA TGGAAGAACG TACAAGACAG ATGAAGATAA AAGTTCAACG GTATAAGCAA
    ATAGCTGGTT CTAGTTCAAG TCAAGAACAG GAACGAGAGC AATATACTAA GTATAACACA
    CACAGAGATC AGTACAGAAG TTGTGACAAC GTCTCAGCCA AATCGTCTGA TAGTGATGTC
    AGTGATGTGT CAGCCATTTC CCGTACCAGT AGTGCCTCAC GTCTCAGTAG CACAAGCTTT
    ATGTCAGAGC AATCTGAACG CCCCCGCAGG AGACTATGTT CCTTTACACC CAGAATGCAA
    AGCAGGCGGA TGGGTACTTC AGGGATAGCC ATAACTAAGA GCACAAGTGT GAGTGGAGAA
    ATTTACAAAC TGGAACACAA TGATGGAAGT CAGTCTGACA CAGCCGTTGG GATGGTTGGG
    ACCGGTGGAA AGAAAAGGAG ATCCAGTCTA AGTGCCAAAG TAGTTGCCAT AGTATCACGA
    AGAAGCAGAA GCACATCCCA GCTTAGCCAA ACAGATTCTG GCAACAAGAA ATTAAAAAGT
    ACTATTCAGA GAAGCACAGA AACAGGCATG GCAGCAGAAA TGAGAAGTCG GATGGTTCGG
    CAACCGAGCC GGGAGTCCAC AGATGGAAGT ATTAACAGTT ACAGCTCAGA GGGCAATTTA
    ATTTTTCCAG GGGTGAGACT GGGGGTTGAT AGCCAATTCA GCGACTTTCT TGATGGACTA
    GGACCAGCAC AACTTGTAGG CAGACAGACA TTAGCAACTC CAGCAATGGG TGATATTCAG
    ATTGGAATGG TAGATAAAAA GGGGCAATTA GAAGTGGAAG TTATACGAGC AAGAGGTCTA
    ATACAAAAGC CAGGATCAAA ATCAACTCCA GCTCCGTATG TAAAGGTATA TCTTTTAGAA
    AATGGAGCGT GTGTCGCCAA GAAAAAGACA AGAATTGCAC GGAAAACCCT TGATCCTCTT
    TATCAACAGA CTTTGGTTTT TGAAGAAAGT CCGAATGGAA AGGTCCTTCA GGTAATTGTC
    TGGGGAGATT ATGGTCGAAT GGATCACAAA TGCTTCATGG GTGTTGCACA GATTTTATTG
    GAAGAACTCG ATCTATCAAG TGTGGTGATT GGATGGTATA AATTGTTTCC GCCATCATCG
    TTGGTGGATC CAACGCTGAC ACCCCTTACT CGCAGGGCTT CCCAATCATC TCTTGAAAGT
    TCTACAGGTC CTCCTTCCAT ACGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57817
    Clone ID Related Accession (Same CDS sequence) XM_031901435.1
    Accession Version XM_031901435.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GATCTCAGCG AGTCAGTGAT
    AGTGACATCT CAGATTTTGA TGTTGATGAT GGTATTGGAG TAGTTCCTCC AGGCTCAAGG
    TCTAATGCTA GAGAAAGTAG ATCTACTACA TTAACAGTGC CAGAACATCA AAGACCAACT
    CATCACCGCT CACGTTCAGT ATCTCCACAC CGTGTTGACG ATCAGGGCAG GACCCGTTCA
    CGTTTACCAA ATGTGCCATT ACAGAGAAGC CTAGATGAAA TCCATCAAAT GAGACGATCA
    CGTTCTCCAA CAAGACACCA TGATGCCTCC CGCAGTCCTG TCGATCGCAG GTCAAGGGAC
    TCAGACAGTC AGTATTTGTC TGAACAAGAA AGTGAACTCC TTATGCTTCC CCGGGCAAAA
    CGTGGACGAA GTGCAGAGTG CCTACATACC ATCAGTGAAC TGCAGCCCTC CCTTGACAGG
    GCTAGAAGTG CTAGTACCAA CTGCTTGAGA CCAGATACTA GTTTGCATTC ACCAGAACGA
    GAAAGGCAGC CCAGAAAACT GGAAAGATCT AGCAACCAGA AACAAAATAG GAAAAGCACT
    GTTTCTGAAA TAGAAAGAAA TCACCAACTC GGAAGCCAGT CATCAGCTGA TTCATCTTTT
    AGTAGTCGAC GAGGAAGGCA GCTTCCACAA GTCCCAGTAA GAAGCAACAG TACAGAACAA
    GCAAATTTAT CGATGGAAGA ACGTACAAGA CAGATGAAGA TAAAAGTTCA ACGGTATAAG
    CAAATAGCTG GTTCTAGTTC AAGTCAAGAA CAGGAACGAG AGCAATATAC TAAGTATAAC
    ACACACAGAG ATCAGTACAG AAGTTGTGAC AACGTCTCAG CCAAATCGTC TGATAGTGAT
    GTCAGTGATG TGTCAGCCAT TTCCCGTACC AGTAGTGCCT CACGTCTCAG TAGCACAAGC
    TTTATGTCAG AGCAATCTGA ACGCCCCCGC AGGAGACTAT GTTCCTTTAC ACCCAGAATG
    CAAAGCAGGC GGATGGGTAC TTCAGGGATA GCCATAACTA AGAGCACAAG TGTGAGTGGA
    GAAATTTACA AACTGGAACA CAATGATGGA AGTCAGTCTG ACACAGCCGT TGGGATGGTT
    GGGACCGGTG GAAAGAAAAG GAGATCCAGT CTAAGTGCCA AAGTAGTTGC CATAGTATCA
    CGAAGAAGCA GAAGCACATC CCAGCTTAGC CAAACAGATT CTGGCAACAA GAAATTAAAA
    AGTACTATTC AGAGAAGCAC AGAAACAGGC ATGGCAGCAG AAATGAGAAG TCGGATGGTT
    CGGCAACCGA GCCGGGAGTC CACAGATGGA AGTATTAACA GTTACAGCTC AGAGGGCAAT
    TTAATTTTTC CAGGGGTGAG ACTGGGGGTT GATAGCCAAT TCAGCGACTT TCTTGATGGA
    CTAGGACCAG CACAACTTGT AGGCAGACAG ACATTAGCAA CTCCAGCAAT GGGTGATATT
    CAGATTGGAA TGGTAGATAA AAAGGGGCAA TTAGAAGTGG AAGTTATACG AGCAAGAGGT
    CTAATACAAA AGCCAGGATC AAAATCAACT CCAGCTCCGT ATGTAAAGGT ATATCTTTTA
    GAAAATGGAG CGTGTGTCGC CAAGAAAAAG ACAAGAATTG CACGGAAAAC CCTTGATCCT
    CTTTATCAAC AGACTTTGGT TTTTGAAGAA AGTCCGAATG GAAAGGTCCT TCAGGTAATT
    GTCTGGGGAG ATTATGGTCG AATGGATCAC AAATGCTTCA TGGGTGTTGC ACAGATTTTA
    TTGGAAGAAC TCGATCTATC AAGTGTGGTG ATTGGATGGT ATAAATTGTT TCCGCCATCA
    TCGTTGGTGG ATCCAACGCT GACACCCCTT ACTCGCAGGG CTTCCCAATC ATCTCTTGAA
    AGTTCTACAG GTCCTCCTTC CATACGATCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757295.1
    CDS362..4894
    Translation

    Target ORF information:

    RefSeq Version XM_031901435.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901435.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GATCTCAGCG AGTCAGTGAT
    AGTGACATCT CAGATTTTGA TGTTGATGAT GGTATTGGAG TAGTTCCTCC AGGCTCAAGG
    TCTAATGCTA GAGAAAGTAG ATCTACTACA TTAACAGTGC CAGAACATCA AAGACCAACT
    CATCACCGCT CACGTTCAGT ATCTCCACAC CGTGTTGACG ATCAGGGCAG GACCCGTTCA
    CGTTTACCAA ATGTGCCATT ACAGAGAAGC CTAGATGAAA TCCATCAAAT GAGACGATCA
    CGTTCTCCAA CAAGACACCA TGATGCCTCC CGCAGTCCTG TCGATCGCAG GTCAAGGGAC
    TCAGACAGTC AGTATTTGTC TGAACAAGAA AGTGAACTCC TTATGCTTCC CCGGGCAAAA
    CGTGGACGAA GTGCAGAGTG CCTACATACC ATCAGTGAAC TGCAGCCCTC CCTTGACAGG
    GCTAGAAGTG CTAGTACCAA CTGCTTGAGA CCAGATACTA GTTTGCATTC ACCAGAACGA
    GAAAGGCAGC CCAGAAAACT GGAAAGATCT AGCAACCAGA AACAAAATAG GAAAAGCACT
    GTTTCTGAAA TAGAAAGAAA TCACCAACTC GGAAGCCAGT CATCAGCTGA TTCATCTTTT
    AGTAGTCGAC GAGGAAGGCA GCTTCCACAA GTCCCAGTAA GAAGCAACAG TACAGAACAA
    GCAAATTTAT CGATGGAAGA ACGTACAAGA CAGATGAAGA TAAAAGTTCA ACGGTATAAG
    CAAATAGCTG GTTCTAGTTC AAGTCAAGAA CAGGAACGAG AGCAATATAC TAAGTATAAC
    ACACACAGAG ATCAGTACAG AAGTTGTGAC AACGTCTCAG CCAAATCGTC TGATAGTGAT
    GTCAGTGATG TGTCAGCCAT TTCCCGTACC AGTAGTGCCT CACGTCTCAG TAGCACAAGC
    TTTATGTCAG AGCAATCTGA ACGCCCCCGC AGGAGACTAT GTTCCTTTAC ACCCAGAATG
    CAAAGCAGGC GGATGGGTAC TTCAGGGATA GCCATAACTA AGAGCACAAG TGTGAGTGGA
    GAAATTTACA AACTGGAACA CAATGATGGA AGTCAGTCTG ACACAGCCGT TGGGATGGTT
    GGGACCGGTG GAAAGAAAAG GAGATCCAGT CTAAGTGCCA AAGTAGTTGC CATAGTATCA
    CGAAGAAGCA GAAGCACATC CCAGCTTAGC CAAACAGATT CTGGCAACAA GAAATTAAAA
    AGTACTATTC AGAGAAGCAC AGAAACAGGC ATGGCAGCAG AAATGAGAAG TCGGATGGTT
    CGGCAACCGA GCCGGGAGTC CACAGATGGA AGTATTAACA GTTACAGCTC AGAGGGCAAT
    TTAATTTTTC CAGGGGTGAG ACTGGGGGTT GATAGCCAAT TCAGCGACTT TCTTGATGGA
    CTAGGACCAG CACAACTTGT AGGCAGACAG ACATTAGCAA CTCCAGCAAT GGGTGATATT
    CAGATTGGAA TGGTAGATAA AAAGGGGCAA TTAGAAGTGG AAGTTATACG AGCAAGAGGT
    CTAATACAAA AGCCAGGATC AAAATCAACT CCAGCTCCGT ATGTAAAGGT ATATCTTTTA
    GAAAATGGAG CGTGTGTCGC CAAGAAAAAG ACAAGAATTG CACGGAAAAC CCTTGATCCT
    CTTTATCAAC AGACTTTGGT TTTTGAAGAA AGTCCGAATG GAAAGGTCCT TCAGGTAATT
    GTCTGGGGAG ATTATGGTCG AATGGATCAC AAATGCTTCA TGGGTGTTGC ACAGATTTTA
    TTGGAAGAAC TCGATCTATC AAGTGTGGTG ATTGGATGGT ATAAATTGTT TCCGCCATCA
    TCGTTGGTGG ATCCAACGCT GACACCCCTT ACTCGCAGGG CTTCCCAATC ATCTCTTGAA
    AGTTCTACAG GTCCTCCTTC CATACGATCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57818
    Clone ID Related Accession (Same CDS sequence) XM_031901436.1
    Accession Version XM_031901436.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGGCAGCCCA GAAAACTGGA AAGATCTAGC
    AACCAGAAAC AAAATAGGAA AAGCACTGTT TCTGAAATAG AAAGAAATCA CCAACTCGGA
    AGCCAGTCAT CAGCTGATTC ATCTTTTAGT AGTCGACGAG GAAGGCAGCT TCCACAAGTC
    CCAGTAAGAA GCAACAGTAC AGAACAAGAA CCAGAGAGTT TTAGTTCATC ATCGAAAGCA
    AATTTATCGA TGGAAGAACG TACAAGACAG ATGAAGATAA AAGTTCAACG GTATAAGCAA
    ATAGCTGGTT CTAGTTCAAG TCAAGAACAG GAACGAGAGC AATATACTAA GTATAACACA
    CACAGAGATC AGTACAGAAG TTGTGACAAC GTCTCAGCCA AATCGTCTGA TAGTGATGTC
    AGTGATGTGT CAGCCATTTC CCGTACCAGT AGTGCCTCAC GTCTCAGTAG CACAAGCTTT
    ATGTCAGAGC AATCTGAACG CCCCCGCAGG AGACTATGTT CCTTTACACC CAGAATGCAA
    AGCAGGCGGA TGGGTACTTC AGGGATAGCC ATAACTAAGA GCACAAGTGT GAGTGGAGAA
    ATTTACAAAC TGGAACACAA TGATGGAAGT CAGTCTGACA CAGCCGTTGG GATGGTTGGG
    ACCGGTGGAA AGAAAAGGAG ATCCAGTCTA AGTGCCAAAG TAGTTGCCAT AGTATCACGA
    AGAAGCAGAA GCACATCCCA GCTTAGCCAA ACAGATTCTG GCAACAAGAA ATTAAAAAGT
    ACTATTCAGA GAAGCACAGA AACAGGCATG GCAGCAGAAA TGAGAAGTCG GATGGTTCGG
    CAACCGAGCC GGGAGTCCAC AGATGGAAGT ATTAACAGTT ACAGCTCAGA GGGCAATTTA
    ATTTTTCCAG GGGTGAGACT GGGGGTTGAT AGCCAATTCA GCGACTTTCT TGATGGACTA
    GGACCAGCAC AACTTGTAGG CAGACAGACA TTAGCAACTC CAGCAATGGG TGATATTCAG
    ATTGGAATGG TAGATAAAAA GGGGCAATTA GAAGTGGAAG TTATACGAGC AAGAGGTCTA
    ATACAAAAGC CAGGATCAAA ATCAACTCCA GCTCCGTATG TAAAGGTATA TCTTTTAGAA
    AATGGAGCGT GTGTCGCCAA GAAAAAGACA AGAATTGCAC GGAAAACCCT TGATCCTCTT
    TATCAACAGA CTTTGGTTTT TGAAGAAAGT CCGAATGGAA AGGTCCTTCA GGTAATTGTC
    TGGGGAGATT ATGGTCGAAT GGATCACAAA TGCTTCATGG GTGTTGCACA GATTTTATTG
    GAAGAACTCG ATCTATCAAG TGTGGTGATT GGATGGTATA AATTGTTTCC GCCATCATCG
    TTGGTGGATC CAACGCTGAC ACCCCTTACT CGCAGGGCTT CCCAATCATC TCTTGAAAGT
    TCTACAGGTC CTCCTTCCAT ACGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757296.1
    CDS362..4891
    Translation

    Target ORF information:

    RefSeq Version XM_031901436.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901436.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG CGGCTCTCTT TGGCAGAAGA
    CAGTTGGAGT GCCCTGTCTC CTCTCAGTCG TGCTGTGGTG ATTTGGACTG TTTTTGGTTG
    GATCCTGCTA CATGGCATAG CAGAGAAACT TCACCCCTAG GGTCGCATCC AGTAACCTGG
    CAGCCATCTA AGGAGGGGGA TCGTTTAATT GGTCGCGTAA TTCTAAATAA AAGAACAAGT
    ATGCCAAAAG AATCTGGATC ACTTCTGGGT TTGAAAGTTG TAGGGGGAAA ATTGTCAGAA
    ACAGGACGCC TTGGTGCCTT CATCACCAAA GTAAAGAAAG GGAGCCTCGC AGATGTTGTT
    GGCCATTTAA GAGCAGGAGA TGAAGTCTTA GAATGGAATG GAAAACCTTT GCCAGGAGCA
    ACAAATGAAG ATGTTTACAA CATTATTTTA CAATCAAAGT CAGAGGCACA AGTTGAAATC
    ATAGTTTCAA GGCCTATTGG AGACATTCCA AGGATTCCAG AAAACTCTCA TCCACCATTA
    GAATCTAGTT CCAGTTCTTT TGAATCTCAA AAGATGGATC GGCCTTCAAT ATCTGTCATT
    TCTCCAACTA GTCCTGGAGC TCTAAAAGAT GCACCTCAGG TCCTGCCAGG ACAGCTTTCT
    GTAAAATTGT GGTATGACAA AGTGGGCCAT CAGCTAATAG TAAATGTCTT GCAAGCAAGA
    GACCTGCCAA CACGGCCAGA TGGAAGGCCT AGGAACCCTT ATGTAAAAAT GTACTTCCTT
    CCGGACAGAA GCGATAAAAG TAAAAGGAGG ACCAAAACAG TGAAGAAAAG CTCTGAACCA
    AAATGGAACC AATCATTTCT CTATTCCCAT GTGCACCGTA GAGACTTTAG AGAGCGAATG
    TTAGAAATAA CTGTGTGGGA TCAGCCAAGA GTACAAGAAG AGGAAAGCGA GTTTCTTGGC
    GAGATCCTAA TAGAACTAGA GACTGCACTA TTAGATGATG AACCTCACTG GTATAAGCTA
    CAGACGCACG ATGAATCTTC ACTACCTCTG CCTCAGCCAT CACCTTTCAT GCCAAGGAGA
    CATGTTCATG GAGAAAATTC TAGCAAAAAA TTACAAAGAT CTCAGCGAGT CAGTGATAGT
    GACATCTCAG ATTTTGATGT TGATGATGGT ATTGGAGTAG TTCCTCCAGG CTCAAGGTCT
    AATGCTAGAG AAAGTAGATC TACTACATTA ACAGTGCCAG AACATCAAAG ACCAACTCAT
    CACCGCTCAC GTTCAGTATC TCCACACCGT GTTGACGATC AGGGCAGGAC CCGTTCACGT
    TTACCAAATG TGCCATTACA GAGAAGCCTA GATGAAATCC ATCAAATGAG ACGATCACGT
    TCTCCAACAA GACACCATGA TGCCTCCCGC AGTCCTGTCG ATCGCAGGTC AAGGGACTCA
    GACAGTCAGT ATTTGTCTGA ACAAGAAAGT GAACTCCTTA TGCTTCCCCG GGCAAAACGT
    GGACGAAGTG CAGAGTGCCT ACATACCATC AGGCAGCCCA GAAAACTGGA AAGATCTAGC
    AACCAGAAAC AAAATAGGAA AAGCACTGTT TCTGAAATAG AAAGAAATCA CCAACTCGGA
    AGCCAGTCAT CAGCTGATTC ATCTTTTAGT AGTCGACGAG GAAGGCAGCT TCCACAAGTC
    CCAGTAAGAA GCAACAGTAC AGAACAAGAA CCAGAGAGTT TTAGTTCATC ATCGAAAGCA
    AATTTATCGA TGGAAGAACG TACAAGACAG ATGAAGATAA AAGTTCAACG GTATAAGCAA
    ATAGCTGGTT CTAGTTCAAG TCAAGAACAG GAACGAGAGC AATATACTAA GTATAACACA
    CACAGAGATC AGTACAGAAG TTGTGACAAC GTCTCAGCCA AATCGTCTGA TAGTGATGTC
    AGTGATGTGT CAGCCATTTC CCGTACCAGT AGTGCCTCAC GTCTCAGTAG CACAAGCTTT
    ATGTCAGAGC AATCTGAACG CCCCCGCAGG AGACTATGTT CCTTTACACC CAGAATGCAA
    AGCAGGCGGA TGGGTACTTC AGGGATAGCC ATAACTAAGA GCACAAGTGT GAGTGGAGAA
    ATTTACAAAC TGGAACACAA TGATGGAAGT CAGTCTGACA CAGCCGTTGG GATGGTTGGG
    ACCGGTGGAA AGAAAAGGAG ATCCAGTCTA AGTGCCAAAG TAGTTGCCAT AGTATCACGA
    AGAAGCAGAA GCACATCCCA GCTTAGCCAA ACAGATTCTG GCAACAAGAA ATTAAAAAGT
    ACTATTCAGA GAAGCACAGA AACAGGCATG GCAGCAGAAA TGAGAAGTCG GATGGTTCGG
    CAACCGAGCC GGGAGTCCAC AGATGGAAGT ATTAACAGTT ACAGCTCAGA GGGCAATTTA
    ATTTTTCCAG GGGTGAGACT GGGGGTTGAT AGCCAATTCA GCGACTTTCT TGATGGACTA
    GGACCAGCAC AACTTGTAGG CAGACAGACA TTAGCAACTC CAGCAATGGG TGATATTCAG
    ATTGGAATGG TAGATAAAAA GGGGCAATTA GAAGTGGAAG TTATACGAGC AAGAGGTCTA
    ATACAAAAGC CAGGATCAAA ATCAACTCCA GCTCCGTATG TAAAGGTATA TCTTTTAGAA
    AATGGAGCGT GTGTCGCCAA GAAAAAGACA AGAATTGCAC GGAAAACCCT TGATCCTCTT
    TATCAACAGA CTTTGGTTTT TGAAGAAAGT CCGAATGGAA AGGTCCTTCA GGTAATTGTC
    TGGGGAGATT ATGGTCGAAT GGATCACAAA TGCTTCATGG GTGTTGCACA GATTTTATTG
    GAAGAACTCG ATCTATCAAG TGTGGTGATT GGATGGTATA AATTGTTTCC GCCATCATCG
    TTGGTGGATC CAACGCTGAC ACCCCTTACT CGCAGGGCTT CCCAATCATC TCTTGAAAGT
    TCTACAGGTC CTCCTTCCAT ACGATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57822
    Clone ID Related Accession (Same CDS sequence) XM_031901443.1
    Accession Version XM_031901443.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GATCTCAGCG AGTCAGTGAT
    AGTGACATCT CAGATTTTGA TGTTGATGAT GGTATTGGAG TAGTTCCTCC AGGCTCAAGG
    TCTAATGCTA GAGAAAGTAG ATCTACTACA TTAACAGTGC CAGAACATCA AAGACCAACT
    CATCACCGCT CACGTTCAGT ATCTCCACAC CGTGTTGACG ATCAGGGCAG GACCCGTTCA
    CGTTTACCAA ATGTGCCATT ACAGAGAAGC CTAGATGAAA TCCATCAAAT GAGACGATCA
    CGTTCTCCAA CAAGACACCA TGATGCCTCC CGCAGTCCTG TCGATCGCAG GTCAAGGGAC
    TCAGACAGTC AGTATTTGTC TGAACAAGAA AGTGAACTCC TTATGCTTCC CCGGGCAAAA
    CGTGGACGAA GTGCAGAGTG CCTACATACC ATCAGAAATA CTGTAAGGCA CAGTAAAACA
    TTACCACCCA AGATGCCTTT ACTAGAGTAT GGTTCTCACT GGAGACTCTA CAGTGAACTG
    CAGCCCTCCC TTGACAGGGC TAGAAGTGCT AGTACCAACT GCTTGAGACC AGATACTAGT
    TTGCATTCAC CAGAACGAGA AAGGCAGCCC AGAAAACTGG AAAGATCTAG CAACCAGAAA
    CAAAATAGGA AAAGCACTGT TTCTGAAATA GAAAGAAATC ACCAACTCGG AAGCCAGTCA
    TCAGCTGATT CATCTTTTAG TAGTCGACGA GGAAGGCAGC TTCCACAAGT CCCAGTAAGA
    AGCAACAGTA CAGAACAAGC AAATTTATCG ATGGAAGAAC GTACAAGACA GATGAAGATA
    AAAGTTCAAC GGTATAAGCA AATAGCTGGT TCTAGTTCAA GTCAAGAACA GGAACGAGAG
    CAATATACTA AGTATAACAC ACACAGAGAT CAGTACAGAA GTTGTGACAA CGTCTCAGCC
    AAATCGTCTG ATAGTGATGT CAGTGATGTG TCAGCCATTT CCCGTACCAG TAGTGCCTCA
    CGTCTCAGTA GCACAAGCTT TATGTCAGAG CAATCTGAAC GCCCCCGCAG GAGACTATGT
    TCCTTTACAC CCAGAATGCA AAGCAGGCGG ATGGGTACTT CAGGGATAGC CATAACTAAG
    AGCACAAGTG TGAGTGGAGA AATTTACAAA CTGGAACACA ATGATGGAAG TCAGTCTGAC
    ACAGCCGTTG GGATGGTTGG GACCGGTGGA AAGAAAAGGA GATCCAGTCT AAGTGCCAAA
    GTAGTTGCCA TAGTATCACG AAGAAGCAGA AGCACATCCC AGCTTAGCCA AACAGATTCT
    GGCAACAAGA AATTAAAAAG TACTATTCAG AGAAGCACAG AAACAGGCAT GGCAGCAGAA
    ATGAGAAGTC GGATGGTTCG GCAACCGAGC CGGGAGTCCA CAGATGGAAG TATTAACAGT
    TACAGCTCAG AGGGCAATTT AATTTTTCCA GGGGTGAGAC TGGGGGTTGA TAGCCAATTC
    AGCGACTTTC TTGATGGACT AGGACCAGCA CAACTTGTAG GCAGACAGAC ATTAGCAACT
    CCAGCAATGG GTGATATTCA GATTGGAATG GTAGATAAAA AGGGGCAATT AGAAGTGGAA
    GTTATACGAG CAAGAGGTCT AATACAAAAG CCAGGATCAA AATCAACTCC AGCTCCGTAT
    GTAAAGGTAT ATCTTTTAGA AAATGGAGCG TGTGTCGCCA AGAAAAAGAC AAGAATTGCA
    CGGAAAACCC TTGATCCTCT TTATCAACAG ACTTTGGTTT TTGAAGAAAG TCCGAATGGA
    AAGGTCCTTC AGGTAATTGT CTGGGGAGAT TATGGTCGAA TGGATCACAA ATGCTTCATG
    GGTGTTGCAC AGATTTTATT GGAAGAACTC GATCTATCAA GTGTGGTGAT TGGATGGTAT
    AAATTGTTTC CGCCATCATC GTTGGTGGAT CCAACGCTGA CACCCCTTAC TCGCAGGGCT
    TCCCAATCAT CTCTTGAAAG TTCTACAGGT CCTCCTTCCA TACGATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757303.1
    CDS362..4972
    Translation

    Target ORF information:

    RefSeq Version XM_031901443.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGA GGACAAAGTG GTTATGTGGG TTTGCAATTT GTGTCGGAAG
    CAACAGGAAA TTTTGACAAA ATCTGGGGAC TGGTTCTTTG GAAGTGGGGT GCAGCAGCCA
    TCTTCTCAGG ATGGAACATT AAGTGACACT GCTACAGGAG CAAGTACGGA TGCACCAAGG
    GAAAAGAAAG CTAGACTTCA AGAGAGATCT CGATCTCAAA CGCCTCTAAG CACCGCTGCA
    GTGGCAGGTT CACAAGGAAC CCCTCCTTCT AGTTTACAGT CTGATAGGAG CAAAGGCTCA
    GAACCATCAC TACAATCCTC AGTCCAAGAG CCAAAATTAT CATCAAGATC AAGAAGTGAA
    CCTCCAAAAG ACAGGAAGAA GGCCTTAGCA ATTTCAGAGC AAAATGGAAA AGTCTTGAAA
    AGTGATCGAA AAAGAGTTCA GAAGTGTTTA AACCAACAAG CTGAGGGACA TATGGATGAC
    AGAGAACATA AAGAAAGGAG AGAAAGCAGA AGATTAGGGA AAGGAAGGTC CCAGGACATT
    CCAGAAAAAG CAGAGGAAGA TACAAACATG GAAGAAGACA AACAAAGAAC AGAGGATGAG
    TATCAAACAC GATACAGAAG TGATCCTAAT TTAGCCAGGT ATCCAGTAAA ACCACAACCA
    GAAGAACAGC AAATGCGCAT GCATGCAAAG GTTTCAAAGG CAAGGCATGA ACGACGGCAC
    AGTGATGCAT CTTTACCACA TACAGAGCTA GATGAAGCTG AAGTTCCTGA GAATAAACTA
    GGCAAAAACT CACAAGGATC AGCATCTCAG GACAAAAAAT CAGTTTTGGA GAATAAGAGG
    TCTTATTCTG TAGACAGAAA TGGTGAAGTA TTAGTTTCCA AGCCAAAACA ATCTGCCAAT
    CACAGTCTCC ATAGTTCTCA ACACAGTCCG GTTCCTCCAG AACATGTAGA CTCTATGAAC
    CATGATTCCT TTAAAAAACA GAGTAGATTA GACCCAAGTT CTGCTCTTTT AACAAGGAAA
    GCTACACGTG AAAAGATGGA GACAATGTTG AGAAATGACT CCCTTAGTTC CGATCAATCA
    GAATCTGTTA GACCTTCCCC ACCAAAGCCC CACAGGTCGA AAAGAGGAGG GAAAAAAAGA
    CAAATGTCAG TTAGTAGCTC AGATGATGAA GGAGCATCAA CCCCGGAATA TACAAGCTGT
    GAAGATGCAG AAATAGAAAG TGAAAGTGTT AGTGAAAAAG GTGATTTGGA CTGTTTTTGG
    TTGGATCCTG CTACATGGCA TAGCAGAGAA ACTTCACCCC TAGGGTCGCA TCCAGTAACC
    TGGCAGCCAT CTAAGGAGGG GGATCGTTTA ATTGGTCGCG TAATTCTAAA TAAAAGAACA
    AGTATGCCAA AAGAATCTGG ATCACTTCTG GGTTTGAAAG TTGTAGGGGG AAAATTGTCA
    GAAACAGGAC GCCTTGGTGC CTTCATCACC AAAGTAAAGA AAGGGAGCCT CGCAGATGTT
    GTTGGCCATT TAAGAGCAGG AGATGAAGTC TTAGAATGGA ATGGAAAACC TTTGCCAGGA
    GCAACAAATG AAGATGTTTA CAACATTATT TTACAATCAA AGTCAGAGGC ACAAGTTGAA
    ATCATAGTTT CAAGGCCTAT TGGAGACATT CCAAGGATTC CAGAAAACTC TCATCCACCA
    TTAGAATCTA GTTCCAGTTC TTTTGAATCT CAAAAGATGG ATCGGCCTTC AATATCTGTC
    ATTTCTCCAA CTAGTCCTGG AGCTCTAAAA GATGCACCTC AGGTCCTGCC AGGACAGCTT
    TCTGTAAAAT TGTGGTATGA CAAAGTGGGC CATCAGCTAA TAGTAAATGT CTTGCAAGCA
    AGAGACCTGC CAACACGGCC AGATGGAAGG CCTAGGAACC CTTATGTAAA AATGTACTTC
    CTTCCGGACA GAAGCGATAA AAGTAAAAGG AGGACCAAAA CAGTGAAGAA AAGCTCTGAA
    CCAAAATGGA ACCAATCATT TCTCTATTCC CATGTGCACC GTAGAGACTT TAGAGAGCGA
    ATGTTAGAAA TAACTGTGTG GGATCAGCCA AGAGTACAAG AAGAGGAAAG CGAGTTTCTT
    GGCGAGATCC TAATAGAACT AGAGACTGCA CTATTAGATG ATGAACCTCA CTGGTATAAG
    CTACAGACGC ACGATGAATC TTCACTACCT CTGCCTCAGC CATCACCTTT CATGCCAAGG
    AGACATGTTC ATGGAGAAAA TTCTAGCAAA AAATTACAAA GATCTCAGCG AGTCAGTGAT
    AGTGACATCT CAGATTTTGA TGTTGATGAT GGTATTGGAG TAGTTCCTCC AGGCTCAAGG
    TCTAATGCTA GAGAAAGTAG ATCTACTACA TTAACAGTGC CAGAACATCA AAGACCAACT
    CATCACCGCT CACGTTCAGT ATCTCCACAC CGTGTTGACG ATCAGGGCAG GACCCGTTCA
    CGTTTACCAA ATGTGCCATT ACAGAGAAGC CTAGATGAAA TCCATCAAAT GAGACGATCA
    CGTTCTCCAA CAAGACACCA TGATGCCTCC CGCAGTCCTG TCGATCGCAG GTCAAGGGAC
    TCAGACAGTC AGTATTTGTC TGAACAAGAA AGTGAACTCC TTATGCTTCC CCGGGCAAAA
    CGTGGACGAA GTGCAGAGTG CCTACATACC ATCAGAAATA CTGTAAGGCA CAGTAAAACA
    TTACCACCCA AGATGCCTTT ACTAGAGTAT GGTTCTCACT GGAGACTCTA CAGTGAACTG
    CAGCCCTCCC TTGACAGGGC TAGAAGTGCT AGTACCAACT GCTTGAGACC AGATACTAGT
    TTGCATTCAC CAGAACGAGA AAGGCAGCCC AGAAAACTGG AAAGATCTAG CAACCAGAAA
    CAAAATAGGA AAAGCACTGT TTCTGAAATA GAAAGAAATC ACCAACTCGG AAGCCAGTCA
    TCAGCTGATT CATCTTTTAG TAGTCGACGA GGAAGGCAGC TTCCACAAGT CCCAGTAAGA
    AGCAACAGTA CAGAACAAGC AAATTTATCG ATGGAAGAAC GTACAAGACA GATGAAGATA
    AAAGTTCAAC GGTATAAGCA AATAGCTGGT TCTAGTTCAA GTCAAGAACA GGAACGAGAG
    CAATATACTA AGTATAACAC ACACAGAGAT CAGTACAGAA GTTGTGACAA CGTCTCAGCC
    AAATCGTCTG ATAGTGATGT CAGTGATGTG TCAGCCATTT CCCGTACCAG TAGTGCCTCA
    CGTCTCAGTA GCACAAGCTT TATGTCAGAG CAATCTGAAC GCCCCCGCAG GAGACTATGT
    TCCTTTACAC CCAGAATGCA AAGCAGGCGG ATGGGTACTT CAGGGATAGC CATAACTAAG
    AGCACAAGTG TGAGTGGAGA AATTTACAAA CTGGAACACA ATGATGGAAG TCAGTCTGAC
    ACAGCCGTTG GGATGGTTGG GACCGGTGGA AAGAAAAGGA GATCCAGTCT AAGTGCCAAA
    GTAGTTGCCA TAGTATCACG AAGAAGCAGA AGCACATCCC AGCTTAGCCA AACAGATTCT
    GGCAACAAGA AATTAAAAAG TACTATTCAG AGAAGCACAG AAACAGGCAT GGCAGCAGAA
    ATGAGAAGTC GGATGGTTCG GCAACCGAGC CGGGAGTCCA CAGATGGAAG TATTAACAGT
    TACAGCTCAG AGGGCAATTT AATTTTTCCA GGGGTGAGAC TGGGGGTTGA TAGCCAATTC
    AGCGACTTTC TTGATGGACT AGGACCAGCA CAACTTGTAG GCAGACAGAC ATTAGCAACT
    CCAGCAATGG GTGATATTCA GATTGGAATG GTAGATAAAA AGGGGCAATT AGAAGTGGAA
    GTTATACGAG CAAGAGGTCT AATACAAAAG CCAGGATCAA AATCAACTCC AGCTCCGTAT
    GTAAAGGTAT ATCTTTTAGA AAATGGAGCG TGTGTCGCCA AGAAAAAGAC AAGAATTGCA
    CGGAAAACCC TTGATCCTCT TTATCAACAG ACTTTGGTTT TTGAAGAAAG TCCGAATGGA
    AAGGTCCTTC AGGTAATTGT CTGGGGAGAT TATGGTCGAA TGGATCACAA ATGCTTCATG
    GGTGTTGCAC AGATTTTATT GGAAGAACTC GATCTATCAA GTGTGGTGAT TGGATGGTAT
    AAATTGTTTC CGCCATCATC GTTGGTGGAT CCAACGCTGA CACCCCTTAC TCGCAGGGCT
    TCCCAATCAT CTCTTGAAAG TTCTACAGGT CCTCCTTCCA TACGATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa23853
    Clone ID Related Accession (Same CDS sequence) NM_001102710.1
    Accession Version NM_001102710.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC135772.1. ##Evidence-Data-START## Transcript exon combination :: BC135772.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGT TATGTGGGTT TGCAATTTGT GTCGGAAGCA ACAGGAAATT
    TTGACAAAAT CTGGGGACTG GTTCTTTGGA AGTGGGGTGC AGCAGCCATC TTCTCAGGAT
    GGAACATTAA GTGACACTGC TACAGGAGCA AGTACGGATG CACCAAGGGA AAAGAAAGCT
    AGACTTCAAG AGAGATCTCG ATCTCAAACG CCTCTAAGCA CCGCTGCAGT GGCAGGTTCA
    CAAGGAACCC CTCCTTCTAG TTTACAGTCT GATAGGAGCA AAGGCTCAGA ACCATCACTA
    CAATCCTCAG TCCAAGAGCC AAAATTATCA TCAAGATCAA GAAGTGAACC TCCAAAAGAC
    AGGAAGAAGG CCTTAGCAAT TTCAGAGCAA AATGGAAAAG TCTTGAAAAG TGATCGAAAA
    AGAGTTCAGA AGTGTTTAAA CCAACAAGCT GAGGGACATA TGGATGACAG AGAACATAAA
    GAAAGGAGAG AAAGCAGAAG ATTAGGGAAA GGAAGGTCCC AGGACATTCC AGAAAAAGCA
    GAGGAAGATA CAAACATGGA AGAAGACAAA CAAAGAACAG AGGATGAGTA TCAAACACGA
    TACAGAAGTG ATCCTAATTT AGCCAGGTAT CCAGTAAAAC CACAACCAGA AGAACAGCAA
    ATGCGCATGC ATGCAAAGGT TTCAAAGGCA AGGCATGAAC GACGGCACAG TGATGCATCT
    TTACCACATA CAGAGCTAGA TGAAGCTGAA GTTCCTGAGA ATAAACTAGG CAAAAACTCA
    CAAGGATCAG CATCTCAGGA CAAAAAATCA GTTTTGGAGA ATAAGAGGTC TTATTCTGTA
    GACAGAAATG GTGAAGTATT AGTTTCCAAG CCAAAACAAT CTGCCAATCA CAGTCTCCAT
    AGTTCTCAAC ACAGTCCGGT TCCTCCAGAA CATGTAGACT CTATGAACCA TGATTCCTTT
    AAAAAACAGA GTAGATTAGA CCCAAGTTCT GCTCTTTTAA CAAGGAAAGC TACACGTGAA
    AAGATGGAGA CAATGTTGAG AAATGACTCC CTTAGTTCCG ATCAATCAGA ATCTGTTAGA
    CCTTCCCCAC CAAAGCCCCA CAGGTCGAAA AGAGAAAAAA AAAAAAGACA AATGTCAGTT
    AGTAGCTCAG ATGATGAAGG AGCATCAACC CCGGAATATA CAAGCTGTGA AGATGCAGAA
    ATAGAAAGTG AAAGTGTTAG TGAAAAAGGT GATTTGGACT GTTTTTGGTT GGATCCTGCT
    ACATGGCATA GCAGAGAAAC TTCACCCCTA GGGTCGCATC CAGTAACCTG GCAGCCATCT
    AAGGAGGGGG ATCGTTTAAT TGGTCGCGTA ATTCTAAATA AAAGAACAAG TATGCCAAAA
    GAATCTGGAT CACTTCTGGG TTTGAAAGTT GTAGGGGGAA AAATGTCAGA AACAGGACGC
    CTTGGTGCCT TCATCACCAA AGTAAAGAAA GGGAGCCTCG CAGATGTTGT TGGCCATTTA
    AGAGCAGGAG ATGAAGTCTT AGAATGGAAT GGAAAACCTT TGCCAGGAGC AACAAATGAA
    GATGTTTACA ACATTATTTT ACAATCAAAG TCAGAGGCAC AAGTTGAAAT CATAGTTTCA
    AGGCCTATTG GAGACATTCC AAGGATTCCA GAAAACTCTC ATCCACCATT AGAATCTAGT
    TCCAGTTCTT TTGAATCTCA AAAGATGGAT CGGCCTTCAA TATCTGTCAT TTCTCCAACT
    AGTCCTGGAG CTCTAAAAGA TGCACCTCAG GTCCTGCCAG GACAGCTTTC TGTAAAATTG
    TGGTATGACA AAGTGGGCCA TCAGCTAATA GTAAATGTCT TGCAAGCAAG AGACCTGCCA
    ACACGGCCAG ATGGAAGGCC TAGGAACCCT TATGTAAAAA TGTACTTCCT TCCGGACAGA
    AGCGATAAAA GTAAAAGGAG GACCAAAACA GTGAAGAAAA GCTCTGAACC AAAATGGAAC
    CAATCATTTC TCTATTCCCA TGTGCACCGT AGAGACTTTA GAGAGCGAAT GTTAGAAATA
    ACTGTGTGGG ATCAGCCAAG AGTACAAGAA GAGGAAAGCG AGTTTCTTGG CGAGATCCTA
    ATAGAACTAG AGACTGCACT ATTAGATGAT GAACCTCACT GGTATAAGCT ACAGACGCAC
    GATGAATCTT CACTACCTCT GCCTCAGCCA TCACCTTTCA TGCCAAGGAG ACATGTTCAT
    GGAGAAAATT CTAGCAAAAA ATTACAAAGC TCAAGGTCTA ATGCTAGAGA AAGTAGATCT
    ACTACATTAA CAGTGCCAGA ACATCAAAGA CCAACTCATC ACCGCTCACG TTCAGTATCT
    CCACACCGTG TTGACGATCA GGGCAGGACC CGTTCACGTT TACCAAATGT GCCATTACAG
    AGAAGCCTAG ATGAAATCCA TCAAATGAGA CGATCACGTT CTCCAACAAG ACACCATGAT
    GCCTCCCGCA GTCCTGTCGA TCGCAGGTCA AGGGACTCAG ACAGTCAGTA TTTGTCTGAA
    CAAGAAAGTG AACTCCTTAT GCTTCCCCGG GCAAAACGTG GACGAAGTGC AGAGTGCCTA
    CATACCATCA GAAATACTGT AAGGCACAGT AAAACATTAC CACCCAAGAT GCCTTTACTA
    GAGTATGGTT CTCACTGGAG ACTCTACAGT GAACTGCAGC CCTCCCTTGA CAGGGCTAGA
    AGTGCTAGTA CCAACTGCTT GAGACCAGAT ACTAGTTTGC ATTCACCAGA ACGAGAAAGG
    CAGCCCAGAA AACTGGAAAG ATCTAGCAAC CAGAAACAAA ATAGGAAAAG CACTGTTTCT
    GAAATAGAAA GAAATCACCA ACTCGGAAGC CAGTCATCAG CTGATTCATC TTTTAGTAGT
    CGACGAGGAA GGCAGCTTCC ACAAGTCCCA GTAAGAAGCA ACAGTACAGA ACAAGAACCA
    GAGAGTTTTA GTTCATCATC GAAAGCAAAT TTATCGATGG AAGAACGTAC AAGACAGATG
    AAGATAAAAG TTCAACGGTA TAAGCAAATA GCTGGTTCTA GTTCAAGTCA AGAACAGGAA
    CGAGAGCAAT ATACTAAGTA TAACACACAC AGAGATCAGT ACAGAAGTTG TGACAACGTC
    TCAGCCAAAT CGTCTGATAG TGATGTCAGT GATGTGTCAG CCATTTCCCG TACCAGTAGT
    GCCTCACGTC TCAGTAGCAC AAGCTTTATG TCAGAGCAAT CTGAACGCCC CCGCAGGAGA
    CTATGTTCCT TTACACCCAG AATGCAAAGC AGGCGGATGG GTACTTCAGG GATAGCCATA
    ACTAAGAGCA CAAGTGTGAG TGGAGAAATT TACAAACTGG AACACAATGA TGGAAGTCAG
    TCTGACACAG CCGTTGGGAT GGTTGGGACC GGTGGAAAGA AAAGGAGATC CAGTCTAAGT
    GCCAAAGTAG TTGCCATAGT ATCACGAAGA AGCAGAAGCA CATCCCAGCT TAGCCAAACA
    GATTCTGGCA ACAAGAAATT AAAAAGTACT ATTCAGAGAA GCACAGAAAC AGGCATGGCA
    GCAGAAATGA GAAGTCGGAT GGTTCGGCAA CCGAGCCGGG AGTCCACAGA TGGAAGTATT
    AACAGTTACA GCTCAGAGGG CAATTTAATT TTTCCAGGGG TGAGACTGGG GGTTGATAGC
    CAATTCAGCG ACTTTCTTGA TGGACTAGGA CCAGCACAAC TTGTAGGCAG ACAGACATTA
    GCAACTCCAG CAATGGGTGA TATTCAGATT GGAATGGTAG ATAAAAAGGG GCAATTAGAA
    GTGGAAGTTA TACGAGCAAG AGGTCTAATA CAAAAGCCAG GATCAAAATC AACTCCAGCT
    CCGTATGTAA AGGTATATCT TTTAGAAAAT GGAGCGTGTG TCGCCAAGAA AAAGACAAGA
    ATTGCACGGA AAACCCTTGA TCCTCTTTAT CAACAGACTT TGGTTTTTGA AGAAAGTCCG
    AATGGAAAGG TCCTTCAGGT AATTGTCTGG GGAGATTATG GTCGAATGGA TCACAAATGC
    TTCATGGGTG TTGCACAGAT TTTATTGGAA GAACTCGATC TATCAAGTGT GGTGATTGGA
    TGGTATAAAT TGTTTCCGCC ATCATCGTTG GTGGATCCAA CGCTGACACC CCTTACTCGC
    AGGGCTTCCC AATCATCTCT TGAAAGTTCT ACAGGTCCTC CTTCCATACG ATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096180.1
    CDS359..4915
    Translation

    Target ORF information:

    RefSeq Version NM_001102710.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 1 (rims1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102710.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCTTCAT CAATGGGGCC CAGGGGCCCT CGGCCACCCA CAGTGCCTCC CCCCATGCAG 
    GATCTCCCCG ATCTCAGCCA TCTGACAGAG GAAGAGAGAA CAATTATCAT GGCAGTGATG
    GACCGACAGA AGCAGGAGGA GGAGAAGGAA GAGGCCATGC TAAAGAGACT CCATCAACAG
    TTTGAAAGCT ATAAAGAGCA AATCAGAAAA ATAGGTGAGG ACACACGCCG TTACCCAGGA
    GAGCACAAGG ACGATGCACC AACGTGTGGA ATCTGCCACA AAACAAAGTT TGCTGATGGC
    TGTGGCCATT TATGCTCCTA TTGCCGTACA AAATTTTGTG CCCGATGTGG AGGTCGCGTT
    TCCCTTCGTT CAAACAATGT TATGTGGGTT TGCAATTTGT GTCGGAAGCA ACAGGAAATT
    TTGACAAAAT CTGGGGACTG GTTCTTTGGA AGTGGGGTGC AGCAGCCATC TTCTCAGGAT
    GGAACATTAA GTGACACTGC TACAGGAGCA AGTACGGATG CACCAAGGGA AAAGAAAGCT
    AGACTTCAAG AGAGATCTCG ATCTCAAACG CCTCTAAGCA CCGCTGCAGT GGCAGGTTCA
    CAAGGAACCC CTCCTTCTAG TTTACAGTCT GATAGGAGCA AAGGCTCAGA ACCATCACTA
    CAATCCTCAG TCCAAGAGCC AAAATTATCA TCAAGATCAA GAAGTGAACC TCCAAAAGAC
    AGGAAGAAGG CCTTAGCAAT TTCAGAGCAA AATGGAAAAG TCTTGAAAAG TGATCGAAAA
    AGAGTTCAGA AGTGTTTAAA CCAACAAGCT GAGGGACATA TGGATGACAG AGAACATAAA
    GAAAGGAGAG AAAGCAGAAG ATTAGGGAAA GGAAGGTCCC AGGACATTCC AGAAAAAGCA
    GAGGAAGATA CAAACATGGA AGAAGACAAA CAAAGAACAG AGGATGAGTA TCAAACACGA
    TACAGAAGTG ATCCTAATTT AGCCAGGTAT CCAGTAAAAC CACAACCAGA AGAACAGCAA
    ATGCGCATGC ATGCAAAGGT TTCAAAGGCA AGGCATGAAC GACGGCACAG TGATGCATCT
    TTACCACATA CAGAGCTAGA TGAAGCTGAA GTTCCTGAGA ATAAACTAGG CAAAAACTCA
    CAAGGATCAG CATCTCAGGA CAAAAAATCA GTTTTGGAGA ATAAGAGGTC TTATTCTGTA
    GACAGAAATG GTGAAGTATT AGTTTCCAAG CCAAAACAAT CTGCCAATCA CAGTCTCCAT
    AGTTCTCAAC ACAGTCCGGT TCCTCCAGAA CATGTAGACT CTATGAACCA TGATTCCTTT
    AAAAAACAGA GTAGATTAGA CCCAAGTTCT GCTCTTTTAA CAAGGAAAGC TACACGTGAA
    AAGATGGAGA CAATGTTGAG AAATGACTCC CTTAGTTCCG ATCAATCAGA ATCTGTTAGA
    CCTTCCCCAC CAAAGCCCCA CAGGTCGAAA AGAGAAAAAA AAAAAAGACA AATGTCAGTT
    AGTAGCTCAG ATGATGAAGG AGCATCAACC CCGGAATATA CAAGCTGTGA AGATGCAGAA
    ATAGAAAGTG AAAGTGTTAG TGAAAAAGGT GATTTGGACT GTTTTTGGTT GGATCCTGCT
    ACATGGCATA GCAGAGAAAC TTCACCCCTA GGGTCGCATC CAGTAACCTG GCAGCCATCT
    AAGGAGGGGG ATCGTTTAAT TGGTCGCGTA ATTCTAAATA AAAGAACAAG TATGCCAAAA
    GAATCTGGAT CACTTCTGGG TTTGAAAGTT GTAGGGGGAA AAATGTCAGA AACAGGACGC
    CTTGGTGCCT TCATCACCAA AGTAAAGAAA GGGAGCCTCG CAGATGTTGT TGGCCATTTA
    AGAGCAGGAG ATGAAGTCTT AGAATGGAAT GGAAAACCTT TGCCAGGAGC AACAAATGAA
    GATGTTTACA ACATTATTTT ACAATCAAAG TCAGAGGCAC AAGTTGAAAT CATAGTTTCA
    AGGCCTATTG GAGACATTCC AAGGATTCCA GAAAACTCTC ATCCACCATT AGAATCTAGT
    TCCAGTTCTT TTGAATCTCA AAAGATGGAT CGGCCTTCAA TATCTGTCAT TTCTCCAACT
    AGTCCTGGAG CTCTAAAAGA TGCACCTCAG GTCCTGCCAG GACAGCTTTC TGTAAAATTG
    TGGTATGACA AAGTGGGCCA TCAGCTAATA GTAAATGTCT TGCAAGCAAG AGACCTGCCA
    ACACGGCCAG ATGGAAGGCC TAGGAACCCT TATGTAAAAA TGTACTTCCT TCCGGACAGA
    AGCGATAAAA GTAAAAGGAG GACCAAAACA GTGAAGAAAA GCTCTGAACC AAAATGGAAC
    CAATCATTTC TCTATTCCCA TGTGCACCGT AGAGACTTTA GAGAGCGAAT GTTAGAAATA
    ACTGTGTGGG ATCAGCCAAG AGTACAAGAA GAGGAAAGCG AGTTTCTTGG CGAGATCCTA
    ATAGAACTAG AGACTGCACT ATTAGATGAT GAACCTCACT GGTATAAGCT ACAGACGCAC
    GATGAATCTT CACTACCTCT GCCTCAGCCA TCACCTTTCA TGCCAAGGAG ACATGTTCAT
    GGAGAAAATT CTAGCAAAAA ATTACAAAGC TCAAGGTCTA ATGCTAGAGA AAGTAGATCT
    ACTACATTAA CAGTGCCAGA ACATCAAAGA CCAACTCATC ACCGCTCACG TTCAGTATCT
    CCACACCGTG TTGACGATCA GGGCAGGACC CGTTCACGTT TACCAAATGT GCCATTACAG
    AGAAGCCTAG ATGAAATCCA TCAAATGAGA CGATCACGTT CTCCAACAAG ACACCATGAT
    GCCTCCCGCA GTCCTGTCGA TCGCAGGTCA AGGGACTCAG ACAGTCAGTA TTTGTCTGAA
    CAAGAAAGTG AACTCCTTAT GCTTCCCCGG GCAAAACGTG GACGAAGTGC AGAGTGCCTA
    CATACCATCA GAAATACTGT AAGGCACAGT AAAACATTAC CACCCAAGAT GCCTTTACTA
    GAGTATGGTT CTCACTGGAG ACTCTACAGT GAACTGCAGC CCTCCCTTGA CAGGGCTAGA
    AGTGCTAGTA CCAACTGCTT GAGACCAGAT ACTAGTTTGC ATTCACCAGA ACGAGAAAGG
    CAGCCCAGAA AACTGGAAAG ATCTAGCAAC CAGAAACAAA ATAGGAAAAG CACTGTTTCT
    GAAATAGAAA GAAATCACCA ACTCGGAAGC CAGTCATCAG CTGATTCATC TTTTAGTAGT
    CGACGAGGAA GGCAGCTTCC ACAAGTCCCA GTAAGAAGCA ACAGTACAGA ACAAGAACCA
    GAGAGTTTTA GTTCATCATC GAAAGCAAAT TTATCGATGG AAGAACGTAC AAGACAGATG
    AAGATAAAAG TTCAACGGTA TAAGCAAATA GCTGGTTCTA GTTCAAGTCA AGAACAGGAA
    CGAGAGCAAT ATACTAAGTA TAACACACAC AGAGATCAGT ACAGAAGTTG TGACAACGTC
    TCAGCCAAAT CGTCTGATAG TGATGTCAGT GATGTGTCAG CCATTTCCCG TACCAGTAGT
    GCCTCACGTC TCAGTAGCAC AAGCTTTATG TCAGAGCAAT CTGAACGCCC CCGCAGGAGA
    CTATGTTCCT TTACACCCAG AATGCAAAGC AGGCGGATGG GTACTTCAGG GATAGCCATA
    ACTAAGAGCA CAAGTGTGAG TGGAGAAATT TACAAACTGG AACACAATGA TGGAAGTCAG
    TCTGACACAG CCGTTGGGAT GGTTGGGACC GGTGGAAAGA AAAGGAGATC CAGTCTAAGT
    GCCAAAGTAG TTGCCATAGT ATCACGAAGA AGCAGAAGCA CATCCCAGCT TAGCCAAACA
    GATTCTGGCA ACAAGAAATT AAAAAGTACT ATTCAGAGAA GCACAGAAAC AGGCATGGCA
    GCAGAAATGA GAAGTCGGAT GGTTCGGCAA CCGAGCCGGG AGTCCACAGA TGGAAGTATT
    AACAGTTACA GCTCAGAGGG CAATTTAATT TTTCCAGGGG TGAGACTGGG GGTTGATAGC
    CAATTCAGCG ACTTTCTTGA TGGACTAGGA CCAGCACAAC TTGTAGGCAG ACAGACATTA
    GCAACTCCAG CAATGGGTGA TATTCAGATT GGAATGGTAG ATAAAAAGGG GCAATTAGAA
    GTGGAAGTTA TACGAGCAAG AGGTCTAATA CAAAAGCCAG GATCAAAATC AACTCCAGCT
    CCGTATGTAA AGGTATATCT TTTAGAAAAT GGAGCGTGTG TCGCCAAGAA AAAGACAAGA
    ATTGCACGGA AAACCCTTGA TCCTCTTTAT CAACAGACTT TGGTTTTTGA AGAAAGTCCG
    AATGGAAAGG TCCTTCAGGT AATTGTCTGG GGAGATTATG GTCGAATGGA TCACAAATGC
    TTCATGGGTG TTGCACAGAT TTTATTGGAA GAACTCGATC TATCAAGTGT GGTGATTGGA
    TGGTATAAAT TGTTTCCGCC ATCATCGTTG GTGGATCCAA CGCTGACACC CCTTACTCGC
    AGGGCTTCCC AATCATCTCT TGAAAGTTCT ACAGGTCCTC CTTCCATACG ATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.