top2a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following top2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa50260 XM_002942586.5
Latest version!
Xenopus tropicalis DNA topoisomerase II alpha (top2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa30195 XM_012952753.2
Latest version!
Xenopus tropicalis topoisomerase (DNA) II alpha (top2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa17562 XM_002942586.4
Latest version!
Xenopus tropicalis topoisomerase (DNA) II alpha (top2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa50260
    Clone ID Related Accession (Same CDS sequence) XM_002942586.5
    Accession Version XM_002942586.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002942586.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGACAG ATCCTCTGCA GCCTCTTGAG AACAAAACTG TGGGTAAAGT GAAGAAAAGT 
    GATAACAAGC GTCTCTCTGT AGAGCGAATT TACCAAAAGA AGACCCAGTT GGAGCATATA
    TTGCTCCGTC CAGATACCTA CATTGGTTCT GTGGAGCCTG TTACACAGCA AATGTGGGTA
    TTTGATGAAG ATGAAGGACT GAATTGCAGA GACGTAACAT TTGTCCCTGG CTTGTATAAA
    ATCTTTGATG AAATACTGGT GAATGCAGCC GATAATAAAC AGAGGGACCC CACCATGTCC
    TGCATAAAGA TCACCATAAA CCCAGAGAGT AACACAGTGA GTGTGTGGAA TAACGGGAAG
    GGAATTCCTG TTGTTGAACA CAAAGTAGAG AAAGTGTTTG TCCCTGCCCT CATATTTGGC
    CAGTTGCTGA CTTCCAGTAA TTATGATGAT GACCAGAAGA AAGTAACTGG TGGTCGTAAT
    GGATACGGTG CCAAACTCTG TAATATATTC AGTAACAAGT TTACTGTTGA AACTGCATGC
    AGAGAGTATA AGAAAGTATT CAAGCAGACT TGGACCGACA ACATGTCGGA TGCAGCAGAT
    GCAAAGATTA AGCCATTTGA TGGGGAAGAC TACACTTGCA TCACTTTTCA GCCTGATCTG
    AGCAAGTTTA AAATGCAGAT ATTAGACAAA GATATTGTGG CCCTAATGTC CCGCAGAGCT
    TACGATGTTG CTGGCTCTTC TAAAGGAGTA AAGGTTTTCC TAAATGGGAA GAGACTTCCA
    ATATCTGGCT TCCGAAGTTA TGTTGATCTG TACCTGAAAA ATAAACTTGA TGAAACGGGT
    AATCCATTGA AGGTAATGCA CGAGCTGGTG AATGATCGCT GGGAAATTTG TTTGACCATG
    AGTGAAAAGG GCTTCCAGCA GATCAGCTTT GTCAACAGTA TTGCAACCAC AAAAGGTGGC
    AGGCATGTTG ATTATGTCGC AGACCAGATT ATTGCCAAAC TTATTGAGGT TGTGAAAAAG
    AAGAATAAAG GTGGGATCGC TGTGAAACCT TTTCAGGTGA AAAATCATAT GTGGGTTTTT
    GTGAATTCCC TGATTGAAAA TCCAACATTT GACTCTCAGA CCAAGGAGAA TATGACTTTG
    CAGTCTAAGA GTTTTGGATC CAAATGTACG CTGTCGGAGA AATTTATTAA AGGTGCAACA
    AACTGTGGAA TTGTGGAAAG CATTCTAAAC TGGGTCAAAT TCAAAGCACA GACTCAACTA
    AATAAAAAAT GTTCCTCAGT GAAGCACACA AAGATCAAGG GCATTCCTAA ACTGGATGAT
    GCAAACGATG CTGGCAGCAG AAACTCCCTG GACTGCACAC TTATTCTAAC TGAAGGAGAC
    TCAGCCAAGA CATTGGCTGT GTCTGGTCTT GGTGTGGTGG GAAGGGACCG GTATGGTGTT
    TTCCCACTTA GAGGCAAGCT TCTCAATGTC AGGGAGGCCT CTCACAAGCA GATCATGGAG
    AATGCTGAAA TAAACAACAT CATTAAGATC GTGGGTCTGC AATATAAAAA GAATTATGAT
    GATAACGAGT CCCTAAAGAC ACTCCGTTAT GGAAAGGTCA TGATTATGAC AGATCAGGAT
    CAAGACGGCT CCCATATCAA AGGCTTGTTG ATCAACTTTA TCCACCACAA TTGGCCATCT
    CTGCTGAAAC ATCGATTTTT GGAGGAATTT ATCACGCCAA TCATCAAGGT CACAAAAAGC
    AAACAGGAGC TTTCATTTTA TAGTATCCCT GAATTTGAGG AATGGAAGAA CAATACTGAC
    AGTCACAAAA CATGGAAAAT AAAGTACTAC AAAGGTCTGG GTACCAGCAC ATCTAAAGAG
    GCAAAGGAAT ACTTCTCCGA TATGGAGAGA CATCGAATTC CCTTCAAATA TGAGGGCCCT
    ATCGATGATG CTGCGATCAC CTTGGCCTTT AGCCGAAAAA AGGTGGATGA CCGTAAGGAA
    TGGCTGACCA ACTTCATGCA AGACCGCAGG GAAAGGAGGC TGCATGGCTT GCCAGAGGAA
    TACCTCTATG GAAAATCCAC AAAATATTTA ACATTCAATG ACTTTATTAA TAAGGAACTG
    ATTCTGTTCT CAAACTCGGA CAATGAAAGA TCCATCCCTT CCCTGGTTGA TGGTTTGAAA
    CCTGGGCAGA GGAAAGTGTT GTTCACTTGC TTTAAAAGAA ATGATAAGCG TGAAGTTAAG
    GTGGCCCAGT TGGCTGGTTC TGTGGCTGAA ATGTCTGCAT ACCATCATGG TGAGACTTCT
    CTAATGATGA CCATCATCAA CTTGGCACAA AACTTTGTCG GAAGTAACAA CGTCAGCCTC
    TTGCAACCAA TTGGTCAGTT TGGCACTAGG TTGCATGGTG GCAAAGATGC TGCCAGTCCT
    CGATACATCT TCACCATGTT AAGCACCCTG GCTCGACTAC TTTTCCCTGC AATGGATGAC
    AGTGTCTTAA AGTTTATGTA TGACGACAAC CAGAAGGTTG AGCCAGAGTG GTACATTCCC
    ATCATACCCA CAGTTCTGGT GAATGGTGCA GAGGGTATTG GCACAGGCTG GTCGTGCAAA
    ATTCCCAATT ACGACACCCG TGAAATTGTG AGCAATATTG GTCGTTTGCT GGATGGACTT
    GAGCCTCTGC CAATGCTTCC CAGCTATAAA AATTTCAAAG GTTTGATTCA AGAACTTGGG
    CCAAACCAGT ACATTCTGAA TGGTGAGGTG TCTGTGCTCG ATAACACAAC TATAGAAATC
    TCGGAACTTC CAGTCAGGAC GTGGACTCAG AATTATAAGG AGCAGGTTCT AGAGCCTATG
    CTAAATGGCA CAGAGAAAAC ACCACCTCTG ATCACAGACT ATAAGGAGTA CCACACCGAC
    ACCACTGTGA AGTTTGTGGT CAAACTGACT GAGCAAAAGC TAATGGAGGC TGAAGCTGCC
    GGGCTTCACA AAGTCTTTAA GCTGCAGACA CCTCTCACGT GTAATTCTAT GGTGCTATTT
    GACCACGTTG GGTGCTTGAG GAAATATGAA ACAGCGACTG ATATCTTGCG GGAATTTTTT
    GTCCTTCGAC TTAAGTATTA TTCTCTAAGA AAGGACTGGC TTGTAGGCAT GCTAACTGCT
    GAGGCCTCCA GACTTTCCAA CCAGGCCAGA TTCATCTTAG AAAAAATAGA GGGCAAGATC
    GTCATAGAAA ACAAACCAAA GAAAGAGCTT ATACAGATGC TTGTCCAACG AGGATACGAC
    TCGGACCCCG TCAAGTCCTG GAAACAAACT CAGGAGAAGG AAGAAGCCGG TGAACTGTTA
    GAAGAAGAAG AAACAGAAGT CAATACAGCT GGGCCTGATT TCAATTACCT TCTTGGCATG
    CCTTTGTGGA ACCTGAGTAA AGAAAAGAAA GATGAGCTGT GTAAGCAGAG AGACGTCAAG
    AACGCAGAGC TTCAGGACCT GAAAAAGAAA TCACCTTCTG ACTTGTGGAA AGAAGACCTT
    TCTGCATTTG TTGAGGAGCT AGACCGTGTA GAAGCCAAAG AAAGGGAAGA ACAGTCCGTA
    ACAACAGGCA AAGTGGTTAA AGGAAAGAGT GGGAGACCGC AAGTAAAGAA AATGCAATTG
    CAGGAAATTA TGCCATCTCC ACATGGCAGG CGTGTTGTCC CTAAGGTCAC AACAGCCATG
    AAAGCGGAAG CCACCAAAAA GATGAAAAAG AGAATTAAGG GTGAGGATGT TGCTAAGAAG
    ATGGATTTCG AAGACGATGG TGAAGGAGAC AGCTTAAATT CACCAGAACA AAGTCCAGAG
    AGCCTAACGC TTGCCGAAAG ATTAAGCAAA AAAGTTAAAA AAGAGCCAGG CGCAAAGGCT
    GTCAAAGACA AGAAGCAGGC AACGTTACCC TTCAAGCCAG TAAAAAAAGA GGCAAAGAGC
    ACATTCCAGG ATTCAGAGAG CGAGTCCGAG GAAGAAGTGG TGCCACGTGC TGCTCAGCCA
    CGCCGCAATG TTGCAGCTAA AGTGAACTAT ATGTTGAGCT CTGATGAAGA CGGTGAAGAA
    GAGGGAAGCG GCATAAAGAT GCCTCAGGAG AAGAGTGACA GTGAATTCAG TGATTTCAAC
    CCAAAATCTG ATTCAGAAGA TGAATCCTTT GTAGTTGCAA AGCCAGCCAA AAAGCCCAGA
    GCTGCGCCCA AGAAAGCTAA AAAGATTGAA AGCCCAGAAA ACTCTCCACA GAAAGACAAA
    GCAGCTCCAG TAATTGCATT AGATGAAGAT GAGGATACTC CCATAATGAC CAGTGCTCCC
    AGCGCCCCTG CACCGGCTCC CAGTGCCAAA GTGTCAAGTG CTAAAGTGGC AGAGAAGAAA
    CCTGCACCTA AAAAACGAGT CCAGAAACCG AAGACTGCTA AAGTGAACTA TATGTTGAGC
    TCTGATGAAG ACGGTGAAGA AGAGGGAAGT GGCATAAAGA TGCCTCAGGA GAAGAGTGAC
    AGTGAATTCA GTGATTTCAA CCCAAAATCT GATTCAGAAG ATGAATCCTT TGTAGTTGCA
    AAGCCAGCCA AAAAGCCCAG AGCTGCGCCC AAGAAGGCTA AAAAGATTGA AAGCCCAGAA
    AACTCTCCAC AGAAAGACAA AGCAGCTCCA ATAATTGCAT TAGATGAAGA TGAGGATACT
    CCCATAATGA CCAGTGCTCC CAGCGCCCCT GCACCGGCTC CCAGTGCCAA AGTATCGAGT
    GCAAAAGTGG CAGAGAAGAA ACCTGCACCT AAAAAACGAG TCCAGAAACC AAAGACTGAG
    GACAGACAGC CGTCGATTTT GGAAGCTCTA AGTAAGCCAA AACCATCAAA GTCTAAATCA
    ACTGCAAAGA AACCTTCATC TGATTCAGAG GATGCTGCTC CGGTCCCTAA AAAGGCAGCG
    AAGGCAACTA AACCAAGAAA GAGGAAGAAC TCTGATAGTG ATTCCGGCTC TGATTTCGGG
    CCAAAGAAGA AAAGCAAATC TACAGCAGCG AAGTCAAAGC CAGCGGAAAA TAAAATAACC
    ATTGAATTCT CCGATTCAGA TTGTGACATG GAGAATATTG TACCCCGTGC CAGAACCGGT
    CGGCAAACGA AACCCGTGAC CTACCTAGAG GATTCGGATG ACGACCTGTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002942632.3
    CDS151..5244
    Translation

    Target ORF information:

    RefSeq Version XM_002942586.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase II alpha (top2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002942586.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGACAG ATCCTCTGCA GCCTCTTGAG AACAAAACTG TGGGTAAAGT GAAGAAAAGT 
    GATAACAAGC GTCTCTCTGT AGAGCGAATT TACCAAAAGA AGACCCAGTT GGAGCATATA
    TTGCTCCGTC CAGATACCTA CATTGGTTCT GTGGAGCCTG TTACACAGCA AATGTGGGTA
    TTTGATGAAG ATGAAGGACT GAATTGCAGA GACGTAACAT TTGTCCCTGG CTTGTATAAA
    ATCTTTGATG AAATACTGGT GAATGCAGCC GATAATAAAC AGAGGGACCC CACCATGTCC
    TGCATAAAGA TCACCATAAA CCCAGAGAGT AACACAGTGA GTGTGTGGAA TAACGGGAAG
    GGAATTCCTG TTGTTGAACA CAAAGTAGAG AAAGTGTTTG TCCCTGCCCT CATATTTGGC
    CAGTTGCTGA CTTCCAGTAA TTATGATGAT GACCAGAAGA AAGTAACTGG TGGTCGTAAT
    GGATACGGTG CCAAACTCTG TAATATATTC AGTAACAAGT TTACTGTTGA AACTGCATGC
    AGAGAGTATA AGAAAGTATT CAAGCAGACT TGGACCGACA ACATGTCGGA TGCAGCAGAT
    GCAAAGATTA AGCCATTTGA TGGGGAAGAC TACACTTGCA TCACTTTTCA GCCTGATCTG
    AGCAAGTTTA AAATGCAGAT ATTAGACAAA GATATTGTGG CCCTAATGTC CCGCAGAGCT
    TACGATGTTG CTGGCTCTTC TAAAGGAGTA AAGGTTTTCC TAAATGGGAA GAGACTTCCA
    ATATCTGGCT TCCGAAGTTA TGTTGATCTG TACCTGAAAA ATAAACTTGA TGAAACGGGT
    AATCCATTGA AGGTAATGCA CGAGCTGGTG AATGATCGCT GGGAAATTTG TTTGACCATG
    AGTGAAAAGG GCTTCCAGCA GATCAGCTTT GTCAACAGTA TTGCAACCAC AAAAGGTGGC
    AGGCATGTTG ATTATGTCGC AGACCAGATT ATTGCCAAAC TTATTGAGGT TGTGAAAAAG
    AAGAATAAAG GTGGGATCGC TGTGAAACCT TTTCAGGTGA AAAATCATAT GTGGGTTTTT
    GTGAATTCCC TGATTGAAAA TCCAACATTT GACTCTCAGA CCAAGGAGAA TATGACTTTG
    CAGTCTAAGA GTTTTGGATC CAAATGTACG CTGTCGGAGA AATTTATTAA AGGTGCAACA
    AACTGTGGAA TTGTGGAAAG CATTCTAAAC TGGGTCAAAT TCAAAGCACA GACTCAACTA
    AATAAAAAAT GTTCCTCAGT GAAGCACACA AAGATCAAGG GCATTCCTAA ACTGGATGAT
    GCAAACGATG CTGGCAGCAG AAACTCCCTG GACTGCACAC TTATTCTAAC TGAAGGAGAC
    TCAGCCAAGA CATTGGCTGT GTCTGGTCTT GGTGTGGTGG GAAGGGACCG GTATGGTGTT
    TTCCCACTTA GAGGCAAGCT TCTCAATGTC AGGGAGGCCT CTCACAAGCA GATCATGGAG
    AATGCTGAAA TAAACAACAT CATTAAGATC GTGGGTCTGC AATATAAAAA GAATTATGAT
    GATAACGAGT CCCTAAAGAC ACTCCGTTAT GGAAAGGTCA TGATTATGAC AGATCAGGAT
    CAAGACGGCT CCCATATCAA AGGCTTGTTG ATCAACTTTA TCCACCACAA TTGGCCATCT
    CTGCTGAAAC ATCGATTTTT GGAGGAATTT ATCACGCCAA TCATCAAGGT CACAAAAAGC
    AAACAGGAGC TTTCATTTTA TAGTATCCCT GAATTTGAGG AATGGAAGAA CAATACTGAC
    AGTCACAAAA CATGGAAAAT AAAGTACTAC AAAGGTCTGG GTACCAGCAC ATCTAAAGAG
    GCAAAGGAAT ACTTCTCCGA TATGGAGAGA CATCGAATTC CCTTCAAATA TGAGGGCCCT
    ATCGATGATG CTGCGATCAC CTTGGCCTTT AGCCGAAAAA AGGTGGATGA CCGTAAGGAA
    TGGCTGACCA ACTTCATGCA AGACCGCAGG GAAAGGAGGC TGCATGGCTT GCCAGAGGAA
    TACCTCTATG GAAAATCCAC AAAATATTTA ACATTCAATG ACTTTATTAA TAAGGAACTG
    ATTCTGTTCT CAAACTCGGA CAATGAAAGA TCCATCCCTT CCCTGGTTGA TGGTTTGAAA
    CCTGGGCAGA GGAAAGTGTT GTTCACTTGC TTTAAAAGAA ATGATAAGCG TGAAGTTAAG
    GTGGCCCAGT TGGCTGGTTC TGTGGCTGAA ATGTCTGCAT ACCATCATGG TGAGACTTCT
    CTAATGATGA CCATCATCAA CTTGGCACAA AACTTTGTCG GAAGTAACAA CGTCAGCCTC
    TTGCAACCAA TTGGTCAGTT TGGCACTAGG TTGCATGGTG GCAAAGATGC TGCCAGTCCT
    CGATACATCT TCACCATGTT AAGCACCCTG GCTCGACTAC TTTTCCCTGC AATGGATGAC
    AGTGTCTTAA AGTTTATGTA TGACGACAAC CAGAAGGTTG AGCCAGAGTG GTACATTCCC
    ATCATACCCA CAGTTCTGGT GAATGGTGCA GAGGGTATTG GCACAGGCTG GTCGTGCAAA
    ATTCCCAATT ACGACACCCG TGAAATTGTG AGCAATATTG GTCGTTTGCT GGATGGACTT
    GAGCCTCTGC CAATGCTTCC CAGCTATAAA AATTTCAAAG GTTTGATTCA AGAACTTGGG
    CCAAACCAGT ACATTCTGAA TGGTGAGGTG TCTGTGCTCG ATAACACAAC TATAGAAATC
    TCGGAACTTC CAGTCAGGAC GTGGACTCAG AATTATAAGG AGCAGGTTCT AGAGCCTATG
    CTAAATGGCA CAGAGAAAAC ACCACCTCTG ATCACAGACT ATAAGGAGTA CCACACCGAC
    ACCACTGTGA AGTTTGTGGT CAAACTGACT GAGCAAAAGC TAATGGAGGC TGAAGCTGCC
    GGGCTTCACA AAGTCTTTAA GCTGCAGACA CCTCTCACGT GTAATTCTAT GGTGCTATTT
    GACCACGTTG GGTGCTTGAG GAAATATGAA ACAGCGACTG ATATCTTGCG GGAATTTTTT
    GTCCTTCGAC TTAAGTATTA TTCTCTAAGA AAGGACTGGC TTGTAGGCAT GCTAACTGCT
    GAGGCCTCCA GACTTTCCAA CCAGGCCAGA TTCATCTTAG AAAAAATAGA GGGCAAGATC
    GTCATAGAAA ACAAACCAAA GAAAGAGCTT ATACAGATGC TTGTCCAACG AGGATACGAC
    TCGGACCCCG TCAAGTCCTG GAAACAAACT CAGGAGAAGG AAGAAGCCGG TGAACTGTTA
    GAAGAAGAAG AAACAGAAGT CAATACAGCT GGGCCTGATT TCAATTACCT TCTTGGCATG
    CCTTTGTGGA ACCTGAGTAA AGAAAAGAAA GATGAGCTGT GTAAGCAGAG AGACGTCAAG
    AACGCAGAGC TTCAGGACCT GAAAAAGAAA TCACCTTCTG ACTTGTGGAA AGAAGACCTT
    TCTGCATTTG TTGAGGAGCT AGACCGTGTA GAAGCCAAAG AAAGGGAAGA ACAGTCCGTA
    ACAACAGGCA AAGTGGTTAA AGGAAAGAGT GGGAGACCGC AAGTAAAGAA AATGCAATTG
    CAGGAAATTA TGCCATCTCC ACATGGCAGG CGTGTTGTCC CTAAGGTCAC AACAGCCATG
    AAAGCGGAAG CCACCAAAAA GATGAAAAAG AGAATTAAGG GTGAGGATGT TGCTAAGAAG
    ATGGATTTCG AAGACGATGG TGAAGGAGAC AGCTTAAATT CACCAGAACA AAGTCCAGAG
    AGCCTAACGC TTGCCGAAAG ATTAAGCAAA AAAGTTAAAA AAGAGCCAGG CGCAAAGGCT
    GTCAAAGACA AGAAGCAGGC AACGTTACCC TTCAAGCCAG TAAAAAAAGA GGCAAAGAGC
    ACATTCCAGG ATTCAGAGAG CGAGTCCGAG GAAGAAGTGG TGCCACGTGC TGCTCAGCCA
    CGCCGCAATG TTGCAGCTAA AGTGAACTAT ATGTTGAGCT CTGATGAAGA CGGTGAAGAA
    GAGGGAAGCG GCATAAAGAT GCCTCAGGAG AAGAGTGACA GTGAATTCAG TGATTTCAAC
    CCAAAATCTG ATTCAGAAGA TGAATCCTTT GTAGTTGCAA AGCCAGCCAA AAAGCCCAGA
    GCTGCGCCCA AGAAAGCTAA AAAGATTGAA AGCCCAGAAA ACTCTCCACA GAAAGACAAA
    GCAGCTCCAG TAATTGCATT AGATGAAGAT GAGGATACTC CCATAATGAC CAGTGCTCCC
    AGCGCCCCTG CACCGGCTCC CAGTGCCAAA GTGTCAAGTG CTAAAGTGGC AGAGAAGAAA
    CCTGCACCTA AAAAACGAGT CCAGAAACCG AAGACTGCTA AAGTGAACTA TATGTTGAGC
    TCTGATGAAG ACGGTGAAGA AGAGGGAAGT GGCATAAAGA TGCCTCAGGA GAAGAGTGAC
    AGTGAATTCA GTGATTTCAA CCCAAAATCT GATTCAGAAG ATGAATCCTT TGTAGTTGCA
    AAGCCAGCCA AAAAGCCCAG AGCTGCGCCC AAGAAGGCTA AAAAGATTGA AAGCCCAGAA
    AACTCTCCAC AGAAAGACAA AGCAGCTCCA ATAATTGCAT TAGATGAAGA TGAGGATACT
    CCCATAATGA CCAGTGCTCC CAGCGCCCCT GCACCGGCTC CCAGTGCCAA AGTATCGAGT
    GCAAAAGTGG CAGAGAAGAA ACCTGCACCT AAAAAACGAG TCCAGAAACC AAAGACTGAG
    GACAGACAGC CGTCGATTTT GGAAGCTCTA AGTAAGCCAA AACCATCAAA GTCTAAATCA
    ACTGCAAAGA AACCTTCATC TGATTCAGAG GATGCTGCTC CGGTCCCTAA AAAGGCAGCG
    AAGGCAACTA AACCAAGAAA GAGGAAGAAC TCTGATAGTG ATTCCGGCTC TGATTTCGGG
    CCAAAGAAGA AAAGCAAATC TACAGCAGCG AAGTCAAAGC CAGCGGAAAA TAAAATAACC
    ATTGAATTCT CCGATTCAGA TTGTGACATG GAGAATATTG TACCCCGTGC CAGAACCGGT
    CGGCAAACGA AACCCGTGAC CTACCTAGAG GATTCGGATG ACGACCTGTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30195
    Clone ID Related Accession (Same CDS sequence) XM_012952753.2
    Accession Version XM_012952753.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683386.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGACAG ATCCTCTGCA GCCTCTTGAG AACAAAACTG TTGGTAAAGT GAAGAAAAGT 
    GATAACAAGC GTCTCTCTGT AGAGCGAATT TACCAAAAGA AGACCCAGTT GGAGCATATA
    TTGCTCCGTC CAGATACCTA CATTGGTTCT GTGGAGCCTG TTACACAGCA AATGTGGGTA
    TTTGATGAAG ATGAAGGACT GAATTGCAGA GACGTAACAT TTGTCCCTGG CTTGTATAAA
    ATCTTTGATG AAATACTGGT GAATGCAGCC GATAATAAAC AGAGGGACCC CACCATGTCC
    TGCATAAAGA TCACCATAAA CCCAGAGAGT AACACAGTGA GTGTGTGGAA TAACGGGAAG
    GGAATTCCTG TTGTTGAACA CAAAGTAGAG AAAGTGTTTG TCCCTGCCCT CATATTTGGC
    CAGTTGCTGA CTTCCAGTAA TTATGATGAT GACCAGAAGA AAGTAACTGG TGGTCGTAAT
    GGATACGGTG CCAAACTCTG TAATATATTC AGTAACAAGT TTACTGTTGA AACTGCATGC
    AGAGAGTATA AGAAAGTATT CAAGCAGACT TGGACCGACA ACATGTCGGA TGCAGCAGAT
    GCAAAGATTA AGCCATTTGA TGGGGAAGAC TACACTTGCA TCACTTTTCA GCCTGATCTG
    AGCAAGTTTA AAATGCAGAT ATTAGACAAA GATATTGTGG CCCTAATGTC CCGCAGAGCT
    TACGATGTTG CTGGCTCTTC TAAAGGAGTA AAGGTTTTCC TAAATGGGAA GAGACTTCCA
    ATATCTGGCT TCCGAAGTTA TGTTGATCTG TACCTGAAAA ATAAACTTGA TGAAACGGGT
    AATCCATTGA AGGTAATGCA CGAGCTGGTG AATGATCGCT GGGAAATTTG TTTGACCATG
    AGTGAAAAGG GCTTCCAGCA GATCAGCTTT GTCAACAGTA TTGCAACCAC AAAAGGTGGC
    AGGCATGTTG ATTATGTCGC AGACCAGATT ATTGCCAAAC TTATTGAGGT TGTGAAAAAG
    AAGAATAAAG GTGGGATCGC TGTGAAACCT TTTCAGGTGA AAAATCATAT GTGGGTTTTT
    GTGAATTCCC TGATTGAAAA TCCAACATTT GACTCTCAGA CCAAGGAGAA TATGACTTTG
    CAGTCTAAGA GTTTTGGATC CAAATGTACG CTGTCGGAGA AATTTATTAA AGGTGCAACA
    AACTGTGGAA TTGTGGAAAG CATTCTAAAC TGGGTCAAAT TCAAAGCACA GACTCAACTA
    AATAAAAAAT GTTCCTCAGT GAAGCACACA AAGATCAAGG GCATTCCTAA ACTGGATGAT
    GCAAACGATG CTGGCAGCAG AAACTCCCTG GACTGCACAC TTATTCTAAC TGAAGGAGAC
    TCAGCCAAGA CATTGGCTGT GTCTGGTCTT GGTGTGGTGG GAAGGGACCG GTATGGTGTT
    TTCCCACTTA GAGGCAAGCT TCTCAATGTC AGGGAGGCCT CTCACAAGCA GATCATGGAG
    AATGCTGAAA TAAACAACAT CATTAAGATC GTGGGTCTGC AATATAAAAA GAATTATGAT
    GATAACGAGT CCCTAAAGAC ACTCCGTTAT GGAAAGGTCA TGATTATGAC AGATCAGGAT
    CAAGACGGCT CCCATATCAA AGGCTTGTTG ATCAACTTTA TCCACCACAA TTGGCCATCT
    CTGCTGAAAC ATCGATTTTT GGAGGAATTT ATCACGCCAA TCATCAAGGT CACAAAAAGC
    AAACAGGAGC TTTCATTTTA TAGTATCCCT GAATTTGAGG AATGGAAGAA CAATACTGAC
    AGTCACAAAA CATGGAAAAT AAAGTACTAC AAAGGTCTGG GTACCAGCAC ATCTAAAGAG
    GCAAAGGAAT ACTTCTCCGA TATGGAGAGA CATCGAATTC CCTTCAAATA TGAGGGCCCT
    ATCGATGATG CTGCGATCAC CTTGGCCTTT AGCCGAAAAA AGGTGGATGA CCGTAAGGAA
    TGGCTGACCA ACTTCATGCA AGACCGCAGG GAAAGGAGGC TGCATGGCTT GCCAGAGGAA
    TACCTCTATG GAAAATCCAC AAAATATTTA ACATTCAATG ACTTTATTAA TAAGGAACTG
    ATTCTGTTCT CAAACTCGGA CAATGAAAGA TCCATCCCTT CCCTGGTTGA TGGTTTGAAA
    CCTGGGCAGA GGAAAGTGTT GTTCACTTGC TTTAAAAGAA ATGATAAGCG TGAAGTTAAG
    GTGGCCCAGT TGGCTGGTTC TGTGGCTGAA ATGTCTGCAT ACCATCATGG TGAGACTTCT
    CTAATGATGA CCATCATCAA CTTGGCACAA AACTTTGTCG GAAGTAACAA CGTCAGCCTC
    TTGCAACCAA TTGGTCAGTT TGGCACTAGG TTGCATGGTG GCAAAGATGC TGCCAGTCCT
    CGATACATCT TCACCATGTT AAGCACCCTG GCTCGACTAC TTTTCCCTGC AATGGATGAC
    AGTGTCTTAA AGTTTATGTA TGACGACAAC CAGAAGGTTG AGCCAGAGTG GTACATTCCC
    ATCATACCCA CAGTTCTGGT GAATGGTGCA GAGGGTATTG GCACAGGCTG GTCGTGCAAA
    ATTCCCAATT ACGACACCCG TGAAATTGTG AGCAATATTG GTCGTTTGCT GGATGGACTT
    GAGCCTCTGC CAATGCTTCC CAGCTATAAA AATTTCAAAG GTTTGATTCA AGAACTTGGG
    CCAAACCAGT ACATTCTGAA TGGTGAGGTG TCTGTGCTCG ATAACACAAC TATAGAAATC
    TCAGAACTTC CAGTCAGGAC GTGGACTCAG AATTATAAGG AGCAGGTTCT AGAGCCTATG
    CTAAATGGCA CAGAGAAAAC ACCACCTCTG ATCACAGACT ATAAGGAGTA CCACACCGAC
    ACCACTGTGA AGTTTGTGGT CAAACTGACT GAGCAAAAGC TAATGGAGGC TGAAGCTGCC
    GGGCTTCACA AAGTCTTTAA GCTGCAGACA CCTCTCACGT GTAATTCTAT GGTGCTATTT
    GACCACGTTG GGTGCTTGAG GAAATATGAA ACAGCAACTG ATATCTTGCG GGAATTTTTT
    GTCCTTCGAC TTAAGTATTA TTCTCTAAGA AAGGACTGGC TTGTAGGCAT GCTAACTGCT
    GAGGCCTCCA GACTTTCCAA CCAGGCCAGA TTCATCTTAG AAAAAATAGA GGGCAAGATC
    GTCATTGAAA ACAAACCAAA GAAAGAGCTT ATACAGATGC TCGTCCAACG AGGATACGAC
    TCGGACCCCG TCAAGTCCTG GAAACAAACT CAGGAGAAGG AAGAAGCCGG TGAACTGTTA
    GAAGAAGAAG AAGAAACAGA AGTCAATACA GCTGGGCCTG ATTTCAATTA CCTTCTTGGC
    ATGCCTTTGT GGAACCTGAG TAAAGAAAAG AAAGATGAGC TGTGTAAGCA GAGAGACGTC
    AAGAACGCAG AGCTTCAGGA CCTGAAAAAG AAATCACCTT CTGACTTGTG GAAAGAAGAC
    CTTTCTGCAT TTGTTGAGGA GCTAGACCGT GTAGAAGCCA AAGAAAGGGA AGAACAGTCC
    GTAACAACAG GCAAAGTGGT TAAAGGAAAG AGTGGGAGAC CGCAAGTAAA GAAAATGCAA
    TTGCAGGAAA TTATGCCATC TCCACATGGC AGGCGTGTTG TCCCTAAGGT CACAACAGCC
    ATGAAAGCGG AAGCCACCAA AAAGATGAAA AAGAGAATTA AGGGTGAGGA TGTTGCTAAG
    AAGATGGATT TCGAAGACGA TGGTGAAGGA GACAGCTTAA ATTCAACAGA ACAAAGTCCA
    GAGAGCCTAA CGCTTGCCGA AAGATTAAGC AAAAAAGTTA AAAAAGAGCC AGGCGCAAAG
    GCTGTCAAAG ACAAGAAGCA GGCAACGTTA CCCTTCAAGC CAGTAAAAAA AGAGGCAAAG
    AGCACATTCC AGGATTCAGA GAGCGAGTCC GAGGAAGAAG TGGTGCCACG TGCTGCTCAG
    CCACGCCGCA ATGTTGCAGC TAAAGTGAAC TATATGTTGA GCTCTGATGA AGACGGTGAA
    GAAGAGGGAA GCGGCATAAA GATGCCCCAG GAGAAGAGTG ACAGTGAATT CAGTGATTTC
    AACCCAAAAT CTGATTCAGA AGATGAATCC TTTGTAGTTG CAAAGCCAGC CAAAAAGCCC
    AGAGCTGCAC CCAAGAAAGC TAAAAAGATT GAAAGCCCAG AAAACTCTCC ACAGAAAGAC
    AAAGCAGCTC CAATAATTGC ATTAGATGAA GATGAGGATA CTCCCATAAT GACCAGTGCT
    CCCAGCGCCC CTGCACCGGC TCCCAGTGCC AAAGTATCGA GTGCAAAAGT GGCAGAGAAG
    AAACCTGCAC CTAAAAAACG AGTCCAGAAA CCAAAGACTG AGGACAGACA GCCGTCGATT
    TTGGAAGCTC TAAGTAAGCC AAAACCATCA AAGTCTAAAT CAACTGCAAA GAAACCTTCA
    TCTGATTCAG AGGATGCTGC TCCGGTCCCT AAAAAGGCAG CGAAGGCAAC TAAACCAAGA
    AAGAGGAAGA ACTCTGATAG TGATTCCGGC TCTGATTTCG GGCCAAAGAA GAAAAGCAAA
    TCTACAGCAG CGAAGTCAAA GCCAGCGGAA AATAAAATAA CCATTGAATT CTCCGATTCA
    GATTGTGACA TGGAGAATAT TGTACCCCGT GCCAGAACCG GTCGGCAAAC GAAACCCGTG
    ACCTACCTAG AGGATTCGGA TGACGACCTG TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012808207.1
    CDS145..4860
    Translation

    Target ORF information:

    RefSeq Version XM_012952753.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) II alpha (top2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012952753.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGACAG ATCCTCTGCA GCCTCTTGAG AACAAAACTG TTGGTAAAGT GAAGAAAAGT 
    GATAACAAGC GTCTCTCTGT AGAGCGAATT TACCAAAAGA AGACCCAGTT GGAGCATATA
    TTGCTCCGTC CAGATACCTA CATTGGTTCT GTGGAGCCTG TTACACAGCA AATGTGGGTA
    TTTGATGAAG ATGAAGGACT GAATTGCAGA GACGTAACAT TTGTCCCTGG CTTGTATAAA
    ATCTTTGATG AAATACTGGT GAATGCAGCC GATAATAAAC AGAGGGACCC CACCATGTCC
    TGCATAAAGA TCACCATAAA CCCAGAGAGT AACACAGTGA GTGTGTGGAA TAACGGGAAG
    GGAATTCCTG TTGTTGAACA CAAAGTAGAG AAAGTGTTTG TCCCTGCCCT CATATTTGGC
    CAGTTGCTGA CTTCCAGTAA TTATGATGAT GACCAGAAGA AAGTAACTGG TGGTCGTAAT
    GGATACGGTG CCAAACTCTG TAATATATTC AGTAACAAGT TTACTGTTGA AACTGCATGC
    AGAGAGTATA AGAAAGTATT CAAGCAGACT TGGACCGACA ACATGTCGGA TGCAGCAGAT
    GCAAAGATTA AGCCATTTGA TGGGGAAGAC TACACTTGCA TCACTTTTCA GCCTGATCTG
    AGCAAGTTTA AAATGCAGAT ATTAGACAAA GATATTGTGG CCCTAATGTC CCGCAGAGCT
    TACGATGTTG CTGGCTCTTC TAAAGGAGTA AAGGTTTTCC TAAATGGGAA GAGACTTCCA
    ATATCTGGCT TCCGAAGTTA TGTTGATCTG TACCTGAAAA ATAAACTTGA TGAAACGGGT
    AATCCATTGA AGGTAATGCA CGAGCTGGTG AATGATCGCT GGGAAATTTG TTTGACCATG
    AGTGAAAAGG GCTTCCAGCA GATCAGCTTT GTCAACAGTA TTGCAACCAC AAAAGGTGGC
    AGGCATGTTG ATTATGTCGC AGACCAGATT ATTGCCAAAC TTATTGAGGT TGTGAAAAAG
    AAGAATAAAG GTGGGATCGC TGTGAAACCT TTTCAGGTGA AAAATCATAT GTGGGTTTTT
    GTGAATTCCC TGATTGAAAA TCCAACATTT GACTCTCAGA CCAAGGAGAA TATGACTTTG
    CAGTCTAAGA GTTTTGGATC CAAATGTACG CTGTCGGAGA AATTTATTAA AGGTGCAACA
    AACTGTGGAA TTGTGGAAAG CATTCTAAAC TGGGTCAAAT TCAAAGCACA GACTCAACTA
    AATAAAAAAT GTTCCTCAGT GAAGCACACA AAGATCAAGG GCATTCCTAA ACTGGATGAT
    GCAAACGATG CTGGCAGCAG AAACTCCCTG GACTGCACAC TTATTCTAAC TGAAGGAGAC
    TCAGCCAAGA CATTGGCTGT GTCTGGTCTT GGTGTGGTGG GAAGGGACCG GTATGGTGTT
    TTCCCACTTA GAGGCAAGCT TCTCAATGTC AGGGAGGCCT CTCACAAGCA GATCATGGAG
    AATGCTGAAA TAAACAACAT CATTAAGATC GTGGGTCTGC AATATAAAAA GAATTATGAT
    GATAACGAGT CCCTAAAGAC ACTCCGTTAT GGAAAGGTCA TGATTATGAC AGATCAGGAT
    CAAGACGGCT CCCATATCAA AGGCTTGTTG ATCAACTTTA TCCACCACAA TTGGCCATCT
    CTGCTGAAAC ATCGATTTTT GGAGGAATTT ATCACGCCAA TCATCAAGGT CACAAAAAGC
    AAACAGGAGC TTTCATTTTA TAGTATCCCT GAATTTGAGG AATGGAAGAA CAATACTGAC
    AGTCACAAAA CATGGAAAAT AAAGTACTAC AAAGGTCTGG GTACCAGCAC ATCTAAAGAG
    GCAAAGGAAT ACTTCTCCGA TATGGAGAGA CATCGAATTC CCTTCAAATA TGAGGGCCCT
    ATCGATGATG CTGCGATCAC CTTGGCCTTT AGCCGAAAAA AGGTGGATGA CCGTAAGGAA
    TGGCTGACCA ACTTCATGCA AGACCGCAGG GAAAGGAGGC TGCATGGCTT GCCAGAGGAA
    TACCTCTATG GAAAATCCAC AAAATATTTA ACATTCAATG ACTTTATTAA TAAGGAACTG
    ATTCTGTTCT CAAACTCGGA CAATGAAAGA TCCATCCCTT CCCTGGTTGA TGGTTTGAAA
    CCTGGGCAGA GGAAAGTGTT GTTCACTTGC TTTAAAAGAA ATGATAAGCG TGAAGTTAAG
    GTGGCCCAGT TGGCTGGTTC TGTGGCTGAA ATGTCTGCAT ACCATCATGG TGAGACTTCT
    CTAATGATGA CCATCATCAA CTTGGCACAA AACTTTGTCG GAAGTAACAA CGTCAGCCTC
    TTGCAACCAA TTGGTCAGTT TGGCACTAGG TTGCATGGTG GCAAAGATGC TGCCAGTCCT
    CGATACATCT TCACCATGTT AAGCACCCTG GCTCGACTAC TTTTCCCTGC AATGGATGAC
    AGTGTCTTAA AGTTTATGTA TGACGACAAC CAGAAGGTTG AGCCAGAGTG GTACATTCCC
    ATCATACCCA CAGTTCTGGT GAATGGTGCA GAGGGTATTG GCACAGGCTG GTCGTGCAAA
    ATTCCCAATT ACGACACCCG TGAAATTGTG AGCAATATTG GTCGTTTGCT GGATGGACTT
    GAGCCTCTGC CAATGCTTCC CAGCTATAAA AATTTCAAAG GTTTGATTCA AGAACTTGGG
    CCAAACCAGT ACATTCTGAA TGGTGAGGTG TCTGTGCTCG ATAACACAAC TATAGAAATC
    TCAGAACTTC CAGTCAGGAC GTGGACTCAG AATTATAAGG AGCAGGTTCT AGAGCCTATG
    CTAAATGGCA CAGAGAAAAC ACCACCTCTG ATCACAGACT ATAAGGAGTA CCACACCGAC
    ACCACTGTGA AGTTTGTGGT CAAACTGACT GAGCAAAAGC TAATGGAGGC TGAAGCTGCC
    GGGCTTCACA AAGTCTTTAA GCTGCAGACA CCTCTCACGT GTAATTCTAT GGTGCTATTT
    GACCACGTTG GGTGCTTGAG GAAATATGAA ACAGCAACTG ATATCTTGCG GGAATTTTTT
    GTCCTTCGAC TTAAGTATTA TTCTCTAAGA AAGGACTGGC TTGTAGGCAT GCTAACTGCT
    GAGGCCTCCA GACTTTCCAA CCAGGCCAGA TTCATCTTAG AAAAAATAGA GGGCAAGATC
    GTCATTGAAA ACAAACCAAA GAAAGAGCTT ATACAGATGC TCGTCCAACG AGGATACGAC
    TCGGACCCCG TCAAGTCCTG GAAACAAACT CAGGAGAAGG AAGAAGCCGG TGAACTGTTA
    GAAGAAGAAG AAGAAACAGA AGTCAATACA GCTGGGCCTG ATTTCAATTA CCTTCTTGGC
    ATGCCTTTGT GGAACCTGAG TAAAGAAAAG AAAGATGAGC TGTGTAAGCA GAGAGACGTC
    AAGAACGCAG AGCTTCAGGA CCTGAAAAAG AAATCACCTT CTGACTTGTG GAAAGAAGAC
    CTTTCTGCAT TTGTTGAGGA GCTAGACCGT GTAGAAGCCA AAGAAAGGGA AGAACAGTCC
    GTAACAACAG GCAAAGTGGT TAAAGGAAAG AGTGGGAGAC CGCAAGTAAA GAAAATGCAA
    TTGCAGGAAA TTATGCCATC TCCACATGGC AGGCGTGTTG TCCCTAAGGT CACAACAGCC
    ATGAAAGCGG AAGCCACCAA AAAGATGAAA AAGAGAATTA AGGGTGAGGA TGTTGCTAAG
    AAGATGGATT TCGAAGACGA TGGTGAAGGA GACAGCTTAA ATTCAACAGA ACAAAGTCCA
    GAGAGCCTAA CGCTTGCCGA AAGATTAAGC AAAAAAGTTA AAAAAGAGCC AGGCGCAAAG
    GCTGTCAAAG ACAAGAAGCA GGCAACGTTA CCCTTCAAGC CAGTAAAAAA AGAGGCAAAG
    AGCACATTCC AGGATTCAGA GAGCGAGTCC GAGGAAGAAG TGGTGCCACG TGCTGCTCAG
    CCACGCCGCA ATGTTGCAGC TAAAGTGAAC TATATGTTGA GCTCTGATGA AGACGGTGAA
    GAAGAGGGAA GCGGCATAAA GATGCCCCAG GAGAAGAGTG ACAGTGAATT CAGTGATTTC
    AACCCAAAAT CTGATTCAGA AGATGAATCC TTTGTAGTTG CAAAGCCAGC CAAAAAGCCC
    AGAGCTGCAC CCAAGAAAGC TAAAAAGATT GAAAGCCCAG AAAACTCTCC ACAGAAAGAC
    AAAGCAGCTC CAATAATTGC ATTAGATGAA GATGAGGATA CTCCCATAAT GACCAGTGCT
    CCCAGCGCCC CTGCACCGGC TCCCAGTGCC AAAGTATCGA GTGCAAAAGT GGCAGAGAAG
    AAACCTGCAC CTAAAAAACG AGTCCAGAAA CCAAAGACTG AGGACAGACA GCCGTCGATT
    TTGGAAGCTC TAAGTAAGCC AAAACCATCA AAGTCTAAAT CAACTGCAAA GAAACCTTCA
    TCTGATTCAG AGGATGCTGC TCCGGTCCCT AAAAAGGCAG CGAAGGCAAC TAAACCAAGA
    AAGAGGAAGA ACTCTGATAG TGATTCCGGC TCTGATTTCG GGCCAAAGAA GAAAAGCAAA
    TCTACAGCAG CGAAGTCAAA GCCAGCGGAA AATAAAATAA CCATTGAATT CTCCGATTCA
    GATTGTGACA TGGAGAATAT TGTACCCCGT GCCAGAACCG GTCGGCAAAC GAAACCCGTG
    ACCTACCTAG AGGATTCGGA TGACGACCTG TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa17562
    Clone ID Related Accession (Same CDS sequence) XM_002942586.4
    Accession Version XM_002942586.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683386.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002942586.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGACAG ATCCTCTGCA GCCTCTTGAG AACAAAACTG TTGGTAAAGT GAAGAAAAGT 
    GATAACAAGC GTCTCTCTGT AGAGCGAATT TACCAAAAGA AGACCCAGTT GGAGCATATA
    TTGCTCCGTC CAGATACCTA CATTGGTTCT GTGGAGCCTG TTACACAGCA AATGTGGGTA
    TTTGATGAAG ATGAAGGACT GAATTGCAGA GACGTAACAT TTGTCCCTGG CTTGTATAAA
    ATCTTTGATG AAATACTGGT GAATGCAGCC GATAATAAAC AGAGGGACCC CACCATGTCC
    TGCATAAAGA TCACCATAAA CCCAGAGAGT AACACAGTGA GTGTGTGGAA TAACGGGAAG
    GGAATTCCTG TTGTTGAACA CAAAGTAGAG AAAGTGTTTG TCCCTGCCCT CATATTTGGC
    CAGTTGCTGA CTTCCAGTAA TTATGATGAT GACCAGAAGA AAGTAACTGG TGGTCGTAAT
    GGATACGGTG CCAAACTCTG TAATATATTC AGTAACAAGT TTACTGTTGA AACTGCATGC
    AGAGAGTATA AGAAAGTATT CAAGCAGACT TGGACCGACA ACATGTCGGA TGCAGCAGAT
    GCAAAGATTA AGCCATTTGA TGGGGAAGAC TACACTTGCA TCACTTTTCA GCCTGATCTG
    AGCAAGTTTA AAATGCAGAT ATTAGACAAA GATATTGTGG CCCTAATGTC CCGCAGAGCT
    TACGATGTTG CTGGCTCTTC TAAAGGAGTA AAGGTTTTCC TAAATGGGAA GAGACTTCCA
    ATATCTGGCT TCCGAAGTTA TGTTGATCTG TACCTGAAAA ATAAACTTGA TGAAACGGGT
    AATCCATTGA AGGTAATGCA CGAGCTGGTG AATGATCGCT GGGAAATTTG TTTGACCATG
    AGTGAAAAGG GCTTCCAGCA GATCAGCTTT GTCAACAGTA TTGCAACCAC AAAAGGTGGC
    AGGCATGTTG ATTATGTCGC AGACCAGATT ATTGCCAAAC TTATTGAGGT TGTGAAAAAG
    AAGAATAAAG GTGGGATCGC TGTGAAACCT TTTCAGGTGA AAAATCATAT GTGGGTTTTT
    GTGAATTCCC TGATTGAAAA TCCAACATTT GACTCTCAGA CCAAGGAGAA TATGACTTTG
    CAGTCTAAGA GTTTTGGATC CAAATGTACG CTGTCGGAGA AATTTATTAA AGGTGCAACA
    AACTGTGGAA TTGTGGAAAG CATTCTAAAC TGGGTCAAAT TCAAAGCACA GACTCAACTA
    AATAAAAAAT GTTCCTCAGT GAAGCACACA AAGATCAAGG GCATTCCTAA ACTGGATGAT
    GCAAACGATG CTGGCAGCAG AAACTCCCTG GACTGCACAC TTATTCTAAC TGAAGGAGAC
    TCAGCCAAGA CATTGGCTGT GTCTGGTCTT GGTGTGGTGG GAAGGGACCG GTATGGTGTT
    TTCCCACTTA GAGGCAAGCT TCTCAATGTC AGGGAGGCCT CTCACAAGCA GATCATGGAG
    AATGCTGAAA TAAACAACAT CATTAAGATC GTGGGTCTGC AATATAAAAA GAATTATGAT
    GATAACGAGT CCCTAAAGAC ACTCCGTTAT GGAAAGGTCA TGATTATGAC AGATCAGGAT
    CAAGACGGCT CCCATATCAA AGGCTTGTTG ATCAACTTTA TCCACCACAA TTGGCCATCT
    CTGCTGAAAC ATCGATTTTT GGAGGAATTT ATCACGCCAA TCATCAAGGT CACAAAAAGC
    AAACAGGAGC TTTCATTTTA TAGTATCCCT GAATTTGAGG AATGGAAGAA CAATACTGAC
    AGTCACAAAA CATGGAAAAT AAAGTACTAC AAAGGTCTGG GTACCAGCAC ATCTAAAGAG
    GCAAAGGAAT ACTTCTCCGA TATGGAGAGA CATCGAATTC CCTTCAAATA TGAGGGCCCT
    ATCGATGATG CTGCGATCAC CTTGGCCTTT AGCCGAAAAA AGGTGGATGA CCGTAAGGAA
    TGGCTGACCA ACTTCATGCA AGACCGCAGG GAAAGGAGGC TGCATGGCTT GCCAGAGGAA
    TACCTCTATG GAAAATCCAC AAAATATTTA ACATTCAATG ACTTTATTAA TAAGGAACTG
    ATTCTGTTCT CAAACTCGGA CAATGAAAGA TCCATCCCTT CCCTGGTTGA TGGTTTGAAA
    CCTGGGCAGA GGAAAGTGTT GTTCACTTGC TTTAAAAGAA ATGATAAGCG TGAAGTTAAG
    GTGGCCCAGT TGGCTGGTTC TGTGGCTGAA ATGTCTGCAT ACCATCATGG TGAGACTTCT
    CTAATGATGA CCATCATCAA CTTGGCACAA AACTTTGTCG GAAGTAACAA CGTCAGCCTC
    TTGCAACCAA TTGGTCAGTT TGGCACTAGG TTGCATGGTG GCAAAGATGC TGCCAGTCCT
    CGATACATCT TCACCATGTT AAGCACCCTG GCTCGACTAC TTTTCCCTGC AATGGATGAC
    AGTGTCTTAA AGTTTATGTA TGACGACAAC CAGAAGGTTG AGCCAGAGTG GTACATTCCC
    ATCATACCCA CAGTTCTGGT GAATGGTGCA GAGGGTATTG GCACAGGCTG GTCGTGCAAA
    ATTCCCAATT ACGACACCCG TGAAATTGTG AGCAATATTG GTCGTTTGCT GGATGGACTT
    GAGCCTCTGC CAATGCTTCC CAGCTATAAA AATTTCAAAG GTTTGATTCA AGAACTTGGG
    CCAAACCAGT ACATTCTGAA TGGTGAGGTG TCTGTGCTCG ATAACACAAC TATAGAAATC
    TCAGAACTTC CAGTCAGGAC GTGGACTCAG AATTATAAGG AGCAGGTTCT AGAGCCTATG
    CTAAATGGCA CAGAGAAAAC ACCACCTCTG ATCACAGACT ATAAGGAGTA CCACACCGAC
    ACCACTGTGA AGTTTGTGGT CAAACTGACT GAGCAAAAGC TAATGGAGGC TGAAGCTGCC
    GGGCTTCACA AAGTCTTTAA GCTGCAGACA CCTCTCACGT GTAATTCTAT GGTGCTATTT
    GACCACGTTG GGTGCTTGAG GAAATATGAA ACAGCAACTG ATATCTTGCG GGAATTTTTT
    GTCCTTCGAC TTAAGTATTA TTCTCTAAGA AAGGACTGGC TTGTAGGCAT GCTAACTGCT
    GAGGCCTCCA GACTTTCCAA CCAGGCCAGA TTCATCTTAG AAAAAATAGA GGGCAAGATC
    GTCATTGAAA ACAAACCAAA GAAAGAGCTT ATACAGATGC TCGTCCAACG AGGATACGAC
    TCGGACCCCG TCAAGTCCTG GAAACAAACT CAGGAGAAGG AAGAAGCCGG TGAACTGTTA
    GAAGAAGAAG AAGAAACAGA AGTCAATACA GCTGGGCCTG ATTTCAATTA CCTTCTTGGC
    ATGCCTTTGT GGAACCTGAG TAAAGAAAAG AAAGATGAGC TGTGTAAGCA GAGAGACGTC
    AAGAACGCAG AGCTTCAGGA CCTGAAAAAG AAATCACCTT CTGACTTGTG GAAAGAAGAC
    CTTTCTGCAT TTGTTGAGGA GCTAGACCGT GTAGAAGCCA AAGAAAGGGA AGAACAGTCC
    GTAACAACAG GCAAAGTGGT TAAAGGAAAG AGTGGGAGAC CGCAAGTAAA GAAAATGCAA
    TTGCAGGAAA TTATGCCATC TCCACATGGC AGGCGTGTTG TCCCTAAGGT CACAACAGCC
    ATGAAAGCGG AAGCCACCAA AAAGATGAAA AAGAGAATTA AGGGTGAGGA TGTTGCTAAG
    AAGATGGATT TCGAAGACGA TGGTGAAGGA GACAGCTTAA ATTCAACAGA ACAAAGTCCA
    GAGAGCCTAA CGCTTGCCGA AAGATTAAGC AAAAAAGTTA AAAAAGAGCC AGGCGCAAAG
    GCTGTCAAAG ACAAGAAGCA GGCAACGTTA CCCTTCAAGC CAGTAAAAAA AGAGGCAAAG
    AGCACATTCC AGGATTCAGA GAGCGAGTCC GAGGAAGAAG TGGTGCCACG TGCTGCTCAG
    CCACGCCGCA ATGTTGCAGC TAAAGTGAAC TATATGTTGA GCTCTGATGA AGACGATGAA
    GAAGAGGGAA GCGGCATAAA GATGCCTCAG GAGAAGAGTG ACAGTGAATT CAGTGATTTC
    AACCCAAAAT CTGATTCAGA AGATGAATCC TTTGTAGTTG CAAAGCCAGC CAAAAAGCCC
    AGAGCTGCAC CCAAGAAAGC TAAAAAGATT GAAAGCCCAG AAAACTCTCC ACAGAAAGAC
    AAAGCAGCTC CAGTAATTGC ATTAGATGAA GATGAGGATA CTCCCATAAT GACCAGTGCT
    CCCAGCGCCC CTGCACCGGC TCCCAGTGCC AAAGTATCGA GTGCAAAAGT GGCAGAGAAG
    AAACCTGCAC CTAAAAAACG AGTCCAGAAA CCGAAGACTG CTAAAGTGAA CTATATGTTG
    AGCTCTGATG AAGACGGTGA AGAAGAGGGA AGCGGCATAA AGATGCCCCA GGAGAAGAGT
    GACAGTGAAT TCAGTGATTT CAACCCAAAA TCTGATTCAG AAGATGAATC CTTTGTAGTT
    GCAAAGCCAG CCAAAAAGCC CAGAGCTGCA CCCAAGAAAG CTAAAAAGAT TGAAAGCCCA
    GAAAACTCTC CACAGAAAGA CAAAGCAGCT CCAATAATTG CATTAGATGA AGATGAGGAT
    ACTCCCATAA TGACCAGTGC TCCCAGCGCC CCTGCACCGG CTCCCAGTGC CAAAGTATCG
    AGTGCAAAAG TGGCAGAGAA GAAACCTGCA CCTAAAAAAC GAGTCCAGAA ACCAAAGACT
    GAGGACAGAC AGCCGTCGAT TTTGGAAGCT CTAAGTAAGC CAAAACCATC AAAGTCTAAA
    TCAACTGCAA AGAAACCTTC ATCTGATTCA GAGGATGCTG CTCCGGTCCC TAAAAAGGCA
    GCGAAGGCAA CTAAACCAAG AAAGAGGAAG AACTCTGATA GTGATTCCGG CTCTGATTTC
    GGGCCAAAGA AGAAAAGCAA ATCTACAGCA GCGAAGTCAA AGCCAGCGGA AAATAAAATA
    ACCATTGAAT TCTCCGATTC AGATTGTGAC ATGGAGAATA TTGTACCCCG TGCCAGAACC
    GGTCGGCAAA CGAAACCCGT GACCTACCTA GAGGATTCGG ATGACGACCT GTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002942632.2
    CDS143..5239
    Translation

    Target ORF information:

    RefSeq Version XM_002942586.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) II alpha (top2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002942586.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGACAG ATCCTCTGCA GCCTCTTGAG AACAAAACTG TTGGTAAAGT GAAGAAAAGT 
    GATAACAAGC GTCTCTCTGT AGAGCGAATT TACCAAAAGA AGACCCAGTT GGAGCATATA
    TTGCTCCGTC CAGATACCTA CATTGGTTCT GTGGAGCCTG TTACACAGCA AATGTGGGTA
    TTTGATGAAG ATGAAGGACT GAATTGCAGA GACGTAACAT TTGTCCCTGG CTTGTATAAA
    ATCTTTGATG AAATACTGGT GAATGCAGCC GATAATAAAC AGAGGGACCC CACCATGTCC
    TGCATAAAGA TCACCATAAA CCCAGAGAGT AACACAGTGA GTGTGTGGAA TAACGGGAAG
    GGAATTCCTG TTGTTGAACA CAAAGTAGAG AAAGTGTTTG TCCCTGCCCT CATATTTGGC
    CAGTTGCTGA CTTCCAGTAA TTATGATGAT GACCAGAAGA AAGTAACTGG TGGTCGTAAT
    GGATACGGTG CCAAACTCTG TAATATATTC AGTAACAAGT TTACTGTTGA AACTGCATGC
    AGAGAGTATA AGAAAGTATT CAAGCAGACT TGGACCGACA ACATGTCGGA TGCAGCAGAT
    GCAAAGATTA AGCCATTTGA TGGGGAAGAC TACACTTGCA TCACTTTTCA GCCTGATCTG
    AGCAAGTTTA AAATGCAGAT ATTAGACAAA GATATTGTGG CCCTAATGTC CCGCAGAGCT
    TACGATGTTG CTGGCTCTTC TAAAGGAGTA AAGGTTTTCC TAAATGGGAA GAGACTTCCA
    ATATCTGGCT TCCGAAGTTA TGTTGATCTG TACCTGAAAA ATAAACTTGA TGAAACGGGT
    AATCCATTGA AGGTAATGCA CGAGCTGGTG AATGATCGCT GGGAAATTTG TTTGACCATG
    AGTGAAAAGG GCTTCCAGCA GATCAGCTTT GTCAACAGTA TTGCAACCAC AAAAGGTGGC
    AGGCATGTTG ATTATGTCGC AGACCAGATT ATTGCCAAAC TTATTGAGGT TGTGAAAAAG
    AAGAATAAAG GTGGGATCGC TGTGAAACCT TTTCAGGTGA AAAATCATAT GTGGGTTTTT
    GTGAATTCCC TGATTGAAAA TCCAACATTT GACTCTCAGA CCAAGGAGAA TATGACTTTG
    CAGTCTAAGA GTTTTGGATC CAAATGTACG CTGTCGGAGA AATTTATTAA AGGTGCAACA
    AACTGTGGAA TTGTGGAAAG CATTCTAAAC TGGGTCAAAT TCAAAGCACA GACTCAACTA
    AATAAAAAAT GTTCCTCAGT GAAGCACACA AAGATCAAGG GCATTCCTAA ACTGGATGAT
    GCAAACGATG CTGGCAGCAG AAACTCCCTG GACTGCACAC TTATTCTAAC TGAAGGAGAC
    TCAGCCAAGA CATTGGCTGT GTCTGGTCTT GGTGTGGTGG GAAGGGACCG GTATGGTGTT
    TTCCCACTTA GAGGCAAGCT TCTCAATGTC AGGGAGGCCT CTCACAAGCA GATCATGGAG
    AATGCTGAAA TAAACAACAT CATTAAGATC GTGGGTCTGC AATATAAAAA GAATTATGAT
    GATAACGAGT CCCTAAAGAC ACTCCGTTAT GGAAAGGTCA TGATTATGAC AGATCAGGAT
    CAAGACGGCT CCCATATCAA AGGCTTGTTG ATCAACTTTA TCCACCACAA TTGGCCATCT
    CTGCTGAAAC ATCGATTTTT GGAGGAATTT ATCACGCCAA TCATCAAGGT CACAAAAAGC
    AAACAGGAGC TTTCATTTTA TAGTATCCCT GAATTTGAGG AATGGAAGAA CAATACTGAC
    AGTCACAAAA CATGGAAAAT AAAGTACTAC AAAGGTCTGG GTACCAGCAC ATCTAAAGAG
    GCAAAGGAAT ACTTCTCCGA TATGGAGAGA CATCGAATTC CCTTCAAATA TGAGGGCCCT
    ATCGATGATG CTGCGATCAC CTTGGCCTTT AGCCGAAAAA AGGTGGATGA CCGTAAGGAA
    TGGCTGACCA ACTTCATGCA AGACCGCAGG GAAAGGAGGC TGCATGGCTT GCCAGAGGAA
    TACCTCTATG GAAAATCCAC AAAATATTTA ACATTCAATG ACTTTATTAA TAAGGAACTG
    ATTCTGTTCT CAAACTCGGA CAATGAAAGA TCCATCCCTT CCCTGGTTGA TGGTTTGAAA
    CCTGGGCAGA GGAAAGTGTT GTTCACTTGC TTTAAAAGAA ATGATAAGCG TGAAGTTAAG
    GTGGCCCAGT TGGCTGGTTC TGTGGCTGAA ATGTCTGCAT ACCATCATGG TGAGACTTCT
    CTAATGATGA CCATCATCAA CTTGGCACAA AACTTTGTCG GAAGTAACAA CGTCAGCCTC
    TTGCAACCAA TTGGTCAGTT TGGCACTAGG TTGCATGGTG GCAAAGATGC TGCCAGTCCT
    CGATACATCT TCACCATGTT AAGCACCCTG GCTCGACTAC TTTTCCCTGC AATGGATGAC
    AGTGTCTTAA AGTTTATGTA TGACGACAAC CAGAAGGTTG AGCCAGAGTG GTACATTCCC
    ATCATACCCA CAGTTCTGGT GAATGGTGCA GAGGGTATTG GCACAGGCTG GTCGTGCAAA
    ATTCCCAATT ACGACACCCG TGAAATTGTG AGCAATATTG GTCGTTTGCT GGATGGACTT
    GAGCCTCTGC CAATGCTTCC CAGCTATAAA AATTTCAAAG GTTTGATTCA AGAACTTGGG
    CCAAACCAGT ACATTCTGAA TGGTGAGGTG TCTGTGCTCG ATAACACAAC TATAGAAATC
    TCAGAACTTC CAGTCAGGAC GTGGACTCAG AATTATAAGG AGCAGGTTCT AGAGCCTATG
    CTAAATGGCA CAGAGAAAAC ACCACCTCTG ATCACAGACT ATAAGGAGTA CCACACCGAC
    ACCACTGTGA AGTTTGTGGT CAAACTGACT GAGCAAAAGC TAATGGAGGC TGAAGCTGCC
    GGGCTTCACA AAGTCTTTAA GCTGCAGACA CCTCTCACGT GTAATTCTAT GGTGCTATTT
    GACCACGTTG GGTGCTTGAG GAAATATGAA ACAGCAACTG ATATCTTGCG GGAATTTTTT
    GTCCTTCGAC TTAAGTATTA TTCTCTAAGA AAGGACTGGC TTGTAGGCAT GCTAACTGCT
    GAGGCCTCCA GACTTTCCAA CCAGGCCAGA TTCATCTTAG AAAAAATAGA GGGCAAGATC
    GTCATTGAAA ACAAACCAAA GAAAGAGCTT ATACAGATGC TCGTCCAACG AGGATACGAC
    TCGGACCCCG TCAAGTCCTG GAAACAAACT CAGGAGAAGG AAGAAGCCGG TGAACTGTTA
    GAAGAAGAAG AAGAAACAGA AGTCAATACA GCTGGGCCTG ATTTCAATTA CCTTCTTGGC
    ATGCCTTTGT GGAACCTGAG TAAAGAAAAG AAAGATGAGC TGTGTAAGCA GAGAGACGTC
    AAGAACGCAG AGCTTCAGGA CCTGAAAAAG AAATCACCTT CTGACTTGTG GAAAGAAGAC
    CTTTCTGCAT TTGTTGAGGA GCTAGACCGT GTAGAAGCCA AAGAAAGGGA AGAACAGTCC
    GTAACAACAG GCAAAGTGGT TAAAGGAAAG AGTGGGAGAC CGCAAGTAAA GAAAATGCAA
    TTGCAGGAAA TTATGCCATC TCCACATGGC AGGCGTGTTG TCCCTAAGGT CACAACAGCC
    ATGAAAGCGG AAGCCACCAA AAAGATGAAA AAGAGAATTA AGGGTGAGGA TGTTGCTAAG
    AAGATGGATT TCGAAGACGA TGGTGAAGGA GACAGCTTAA ATTCAACAGA ACAAAGTCCA
    GAGAGCCTAA CGCTTGCCGA AAGATTAAGC AAAAAAGTTA AAAAAGAGCC AGGCGCAAAG
    GCTGTCAAAG ACAAGAAGCA GGCAACGTTA CCCTTCAAGC CAGTAAAAAA AGAGGCAAAG
    AGCACATTCC AGGATTCAGA GAGCGAGTCC GAGGAAGAAG TGGTGCCACG TGCTGCTCAG
    CCACGCCGCA ATGTTGCAGC TAAAGTGAAC TATATGTTGA GCTCTGATGA AGACGATGAA
    GAAGAGGGAA GCGGCATAAA GATGCCTCAG GAGAAGAGTG ACAGTGAATT CAGTGATTTC
    AACCCAAAAT CTGATTCAGA AGATGAATCC TTTGTAGTTG CAAAGCCAGC CAAAAAGCCC
    AGAGCTGCAC CCAAGAAAGC TAAAAAGATT GAAAGCCCAG AAAACTCTCC ACAGAAAGAC
    AAAGCAGCTC CAGTAATTGC ATTAGATGAA GATGAGGATA CTCCCATAAT GACCAGTGCT
    CCCAGCGCCC CTGCACCGGC TCCCAGTGCC AAAGTATCGA GTGCAAAAGT GGCAGAGAAG
    AAACCTGCAC CTAAAAAACG AGTCCAGAAA CCGAAGACTG CTAAAGTGAA CTATATGTTG
    AGCTCTGATG AAGACGGTGA AGAAGAGGGA AGCGGCATAA AGATGCCCCA GGAGAAGAGT
    GACAGTGAAT TCAGTGATTT CAACCCAAAA TCTGATTCAG AAGATGAATC CTTTGTAGTT
    GCAAAGCCAG CCAAAAAGCC CAGAGCTGCA CCCAAGAAAG CTAAAAAGAT TGAAAGCCCA
    GAAAACTCTC CACAGAAAGA CAAAGCAGCT CCAATAATTG CATTAGATGA AGATGAGGAT
    ACTCCCATAA TGACCAGTGC TCCCAGCGCC CCTGCACCGG CTCCCAGTGC CAAAGTATCG
    AGTGCAAAAG TGGCAGAGAA GAAACCTGCA CCTAAAAAAC GAGTCCAGAA ACCAAAGACT
    GAGGACAGAC AGCCGTCGAT TTTGGAAGCT CTAAGTAAGC CAAAACCATC AAAGTCTAAA
    TCAACTGCAA AGAAACCTTC ATCTGATTCA GAGGATGCTG CTCCGGTCCC TAAAAAGGCA
    GCGAAGGCAA CTAAACCAAG AAAGAGGAAG AACTCTGATA GTGATTCCGG CTCTGATTTC
    GGGCCAAAGA AGAAAAGCAA ATCTACAGCA GCGAAGTCAA AGCCAGCGGA AAATAAAATA
    ACCATTGAAT TCTCCGATTC AGATTGTGAC ATGGAGAATA TTGTACCCCG TGCCAGAACC
    GGTCGGCAAA CGAAACCCGT GACCTACCTA GAGGATTCGG ATGACGACCT GTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.