tcf20 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tcf20
Entrez Gene ID	100038081
Full Name	transcription factor 20 (AR1)
Synonyms	ar1,spbp
General protein information	Preferred Names transcription factor 20 (AR1) Names transcription factor 20
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934712.5	XP_002934758.2	transcription factor 20 isoform X2
XM_002934714.5	XP_002934760.2	transcription factor 20 isoform X1
XM_002934712.4	XP_002934758.1	transcription factor 20 isoform X2
XM_004913793.4	XP_004913850.2	transcription factor 20 isoform X1
XM_004913793.3	XP_004913850.1	transcription factor 20 isoform X1
XM_002934714.4	XP_002934760.1	transcription factor 20 isoform X1
XM_031901057.1	XP_031756917.1	transcription factor 20 isoform X1

Pathways from BioSystems

Homologies

	TCF20	XP_001107318.2
Canis lupus familiaris (dog)	TCF20	XP_857454.2
Rattus norvegicus (Norway rat)	Tcf20	NP_001124046.1
Homo sapiens (human)	TCF20	NP_005641.1
Pan troglodytes (chimpanzee)	TCF20	XP_003317325.1
Mus musculus (house mouse)	Tcf20	NP_001107612.1
Gallus gallus (chicken)	TCF20	XP_004937744.1
Xenopus tropicalis (tropical clawed frog)	tcf20	XP_004913850.1
Bos taurus (cattle)	TCF20	NP_001179503.1

Gene Ontology

The following tcf20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tcf20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa57367	XM_031901057.1 Latest version!	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57367	XM_002934714.5 Latest version!	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57367	XM_004913793.4 Latest version!	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57368	XM_002934712.5 Latest version!	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa09201	XM_004913793.3 Latest version!	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa09201	XM_002934714.4 Latest version!	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa09202	XM_002934712.4 Latest version!	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tcf20 ( XM_002934714.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934760.2 Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa57367
Clone ID Related Accession (Same CDS sequence)	XM_002934714.5 , XM_004913793.4 , XM_031901057.1
Accession Version	XM_002934714.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934714.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCACAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGTT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGACCAAGAC AGTAAAGGGT 
GTTTCATCAG AGCAGCCTGA GCAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934760.2
CDS	323..5872
Translation	MQSFREQSSYGSQQTYPPEAHGPSRLEEFSPRQQAQIFQGNYGGRRGGSSTPATIPAGETSSIQSYQSYRKESGDFYYLGNKEGGTPASQTPQRRPSGPVQSYGPPQANSSSGTNFGTPYGREGHHSQFANQHSAAGNLSQYSQDFPSSFSPGGSQYPSHVTSQQLRQQLYQSHQSLSQSGNPPTSAGASHLQQIQRSTALNSPAGYQLRMGQYGQHYQPSPGSSSSSFPSSQRFGQTGPNYEGYSPGGASSTYESHMPGSSYGTQQGFSSYASQHMKSFETSKLPQNVGQGQSQQQQQTSHVMQYSNSSKLPLQSQTGQYSQSEVPVRSPMQFQQNFSPISNPSPAASVVQSPSCSSTPSPLMTSGENLTCGQGNVPLSSRNRILQMMPQLSPTPHGGGFKGFGVEGPPDKRLTDPGLSSLSALSSQVANLPNTVQHMLLSDALAPNKKSGKRTSRKADSCANSETSSQAEEQLKSPMAESLDGSCSSSSGDPGERVRQLSGQSTSSEAGYKGTSSEKPASPTQGWSTELSEKTQAEEVETVPEATETFQEVSKVSEKSVGVIVAMETVATRSEKVTEESSTLDNDEVETSSQPEVPETVKDDDNVKECPPDQNGEPSDADSQPVPITSSTSRTEAKSPVGSGFSFKEGATSSRNASNLPQHLKTPDSAGCGGQGDKKSGTGRNDKYPSLLQEVLQGHHQQERRYARNTQDPPPVTGSSEASLRPNILMSQASELPNRNILGKTLAPHLETPNWGPWDRKSAPDVKPLNVTDYSARKFEVDPPASSQESSGIPSERRSVICDISPLRQLVRDPTASHHVGQERSADGRSGQSVILPSGMISLDAKSSNQGGTPKEQDTEATKMHRKRTTDHCIPYSTKESARGNGSPRSMTFDPTQDYSPHNSRGPHPRRGTARVGARGRSPVQDLGDKMKMSPGRSRGPAETHHMNPTMSLSERASREAFYSAFFQNADNAALSYHSNSRASSYGEPHPTFTSPLHYKRPVYQQEEFKEWLGSSILSASQHRQEMQRKSPRQEQFHVRSPGRVENEVMVYNQPGAYHDSSSLEYGRQLRVGEGGPTNLTALELKRNAQKTPPVDSTNWNLGRQTSPVKKLGSPVGTNQKPFSSGRETDTLVHRSGEGTEISKPGCNAHRPQGTEEQSHHNPLIMRRRVRSFISPIPSKRQPGDDKGRLPVSNVKETSDRTSVSQVLTSRSKESGFSPVADEPHKGGPEQTSPSAVSLSSPAKTKILPPRKGRGLKLEAIVQKITSPNVRRISAPSSSESAAEAVTLDDILSLKIKGPESGKGSMQEAELPGVVGPDISCSEETTAKSLPPKSLDVWAHDEKKIKTEMEEQSMETKDFSMSGLPAQASNHYMDGKGTSTALEPKCVLPQVQQHENVEKEKLAGTPCNVTPKQEVIPQKGYFPSGKKKGRPVGSVNKQKKQQQQPLMQEQGHQQPPPPVSPASVSALPPQHLDEHLDISEAEPKPKRRRTERRKPTGAPRRRRGKQAAPIVAPIEPEIKLKYASQNLDKPETKTKSFIPYIHVENKEELGLSCVIINAEEEEQHWKKLTSVRKGQRSASPQPSTDSKALPSSSFMVQGPVVTESATMGHLVCCLCGKWANYRNLGDLFGPFYNQEYASTLSKNPPPKKSSETPRKVKVRHKDTSDGSKSDSDEEEEEEPQQAREQRSLTAHPRYKRRNRSGDCTSSRLTVPSHRKTTDPSELTPMDSSGPSTAEAGPEQGVQIPQLPLDSNEFWMHEGCVLWANGVYFVCGRLYGLREAVDIAREMKCSHCQETGATLGCYNKGCACCYHFPCAMDSECLLNEENFSVRCPKHKTKTVKGVSSEQPEQG

Target ORF information:

RefSeq Version	XM_002934714.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934714.5

ORF Insert Sequence:

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCACAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGTT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGACCAAGAC AGTAAAGGGT 
GTTTCATCAG AGCAGCCTGA GCAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcf20 ( XM_004913793.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913850.2 Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X1, mRNA.

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CloneID	OXa57367
Clone ID Related Accession (Same CDS sequence)	XM_002934714.5 , XM_004913793.4 , XM_031901057.1
Accession Version	XM_004913793.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913793.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCACAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGTT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGACCAAGAC AGTAAAGGGT 
GTTTCATCAG AGCAGCCTGA GCAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913850.2
CDS	199..5748
Translation	MQSFREQSSYGSQQTYPPEAHGPSRLEEFSPRQQAQIFQGNYGGRRGGSSTPATIPAGETSSIQSYQSYRKESGDFYYLGNKEGGTPASQTPQRRPSGPVQSYGPPQANSSSGTNFGTPYGREGHHSQFANQHSAAGNLSQYSQDFPSSFSPGGSQYPSHVTSQQLRQQLYQSHQSLSQSGNPPTSAGASHLQQIQRSTALNSPAGYQLRMGQYGQHYQPSPGSSSSSFPSSQRFGQTGPNYEGYSPGGASSTYESHMPGSSYGTQQGFSSYASQHMKSFETSKLPQNVGQGQSQQQQQTSHVMQYSNSSKLPLQSQTGQYSQSEVPVRSPMQFQQNFSPISNPSPAASVVQSPSCSSTPSPLMTSGENLTCGQGNVPLSSRNRILQMMPQLSPTPHGGGFKGFGVEGPPDKRLTDPGLSSLSALSSQVANLPNTVQHMLLSDALAPNKKSGKRTSRKADSCANSETSSQAEEQLKSPMAESLDGSCSSSSGDPGERVRQLSGQSTSSEAGYKGTSSEKPASPTQGWSTELSEKTQAEEVETVPEATETFQEVSKVSEKSVGVIVAMETVATRSEKVTEESSTLDNDEVETSSQPEVPETVKDDDNVKECPPDQNGEPSDADSQPVPITSSTSRTEAKSPVGSGFSFKEGATSSRNASNLPQHLKTPDSAGCGGQGDKKSGTGRNDKYPSLLQEVLQGHHQQERRYARNTQDPPPVTGSSEASLRPNILMSQASELPNRNILGKTLAPHLETPNWGPWDRKSAPDVKPLNVTDYSARKFEVDPPASSQESSGIPSERRSVICDISPLRQLVRDPTASHHVGQERSADGRSGQSVILPSGMISLDAKSSNQGGTPKEQDTEATKMHRKRTTDHCIPYSTKESARGNGSPRSMTFDPTQDYSPHNSRGPHPRRGTARVGARGRSPVQDLGDKMKMSPGRSRGPAETHHMNPTMSLSERASREAFYSAFFQNADNAALSYHSNSRASSYGEPHPTFTSPLHYKRPVYQQEEFKEWLGSSILSASQHRQEMQRKSPRQEQFHVRSPGRVENEVMVYNQPGAYHDSSSLEYGRQLRVGEGGPTNLTALELKRNAQKTPPVDSTNWNLGRQTSPVKKLGSPVGTNQKPFSSGRETDTLVHRSGEGTEISKPGCNAHRPQGTEEQSHHNPLIMRRRVRSFISPIPSKRQPGDDKGRLPVSNVKETSDRTSVSQVLTSRSKESGFSPVADEPHKGGPEQTSPSAVSLSSPAKTKILPPRKGRGLKLEAIVQKITSPNVRRISAPSSSESAAEAVTLDDILSLKIKGPESGKGSMQEAELPGVVGPDISCSEETTAKSLPPKSLDVWAHDEKKIKTEMEEQSMETKDFSMSGLPAQASNHYMDGKGTSTALEPKCVLPQVQQHENVEKEKLAGTPCNVTPKQEVIPQKGYFPSGKKKGRPVGSVNKQKKQQQQPLMQEQGHQQPPPPVSPASVSALPPQHLDEHLDISEAEPKPKRRRTERRKPTGAPRRRRGKQAAPIVAPIEPEIKLKYASQNLDKPETKTKSFIPYIHVENKEELGLSCVIINAEEEEQHWKKLTSVRKGQRSASPQPSTDSKALPSSSFMVQGPVVTESATMGHLVCCLCGKWANYRNLGDLFGPFYNQEYASTLSKNPPPKKSSETPRKVKVRHKDTSDGSKSDSDEEEEEEPQQAREQRSLTAHPRYKRRNRSGDCTSSRLTVPSHRKTTDPSELTPMDSSGPSTAEAGPEQGVQIPQLPLDSNEFWMHEGCVLWANGVYFVCGRLYGLREAVDIAREMKCSHCQETGATLGCYNKGCACCYHFPCAMDSECLLNEENFSVRCPKHKTKTVKGVSSEQPEQG

Target ORF information:

RefSeq Version	XM_004913793.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913793.4

ORF Insert Sequence:

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCACAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGTT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGACCAAGAC AGTAAAGGGT 
GTTTCATCAG AGCAGCCTGA GCAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcf20 ( XM_031901057.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756917.1 Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X3, mRNA.

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CloneID	OXa57367
Clone ID Related Accession (Same CDS sequence)	XM_002934714.5 , XM_004913793.4 , XM_031901057.1
Accession Version	XM_031901057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCACAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGTT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGACCAAGAC AGTAAAGGGT 
GTTTCATCAG AGCAGCCTGA GCAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756917.1
CDS	191..5740
Translation	MQSFREQSSYGSQQTYPPEAHGPSRLEEFSPRQQAQIFQGNYGGRRGGSSTPATIPAGETSSIQSYQSYRKESGDFYYLGNKEGGTPASQTPQRRPSGPVQSYGPPQANSSSGTNFGTPYGREGHHSQFANQHSAAGNLSQYSQDFPSSFSPGGSQYPSHVTSQQLRQQLYQSHQSLSQSGNPPTSAGASHLQQIQRSTALNSPAGYQLRMGQYGQHYQPSPGSSSSSFPSSQRFGQTGPNYEGYSPGGASSTYESHMPGSSYGTQQGFSSYASQHMKSFETSKLPQNVGQGQSQQQQQTSHVMQYSNSSKLPLQSQTGQYSQSEVPVRSPMQFQQNFSPISNPSPAASVVQSPSCSSTPSPLMTSGENLTCGQGNVPLSSRNRILQMMPQLSPTPHGGGFKGFGVEGPPDKRLTDPGLSSLSALSSQVANLPNTVQHMLLSDALAPNKKSGKRTSRKADSCANSETSSQAEEQLKSPMAESLDGSCSSSSGDPGERVRQLSGQSTSSEAGYKGTSSEKPASPTQGWSTELSEKTQAEEVETVPEATETFQEVSKVSEKSVGVIVAMETVATRSEKVTEESSTLDNDEVETSSQPEVPETVKDDDNVKECPPDQNGEPSDADSQPVPITSSTSRTEAKSPVGSGFSFKEGATSSRNASNLPQHLKTPDSAGCGGQGDKKSGTGRNDKYPSLLQEVLQGHHQQERRYARNTQDPPPVTGSSEASLRPNILMSQASELPNRNILGKTLAPHLETPNWGPWDRKSAPDVKPLNVTDYSARKFEVDPPASSQESSGIPSERRSVICDISPLRQLVRDPTASHHVGQERSADGRSGQSVILPSGMISLDAKSSNQGGTPKEQDTEATKMHRKRTTDHCIPYSTKESARGNGSPRSMTFDPTQDYSPHNSRGPHPRRGTARVGARGRSPVQDLGDKMKMSPGRSRGPAETHHMNPTMSLSERASREAFYSAFFQNADNAALSYHSNSRASSYGEPHPTFTSPLHYKRPVYQQEEFKEWLGSSILSASQHRQEMQRKSPRQEQFHVRSPGRVENEVMVYNQPGAYHDSSSLEYGRQLRVGEGGPTNLTALELKRNAQKTPPVDSTNWNLGRQTSPVKKLGSPVGTNQKPFSSGRETDTLVHRSGEGTEISKPGCNAHRPQGTEEQSHHNPLIMRRRVRSFISPIPSKRQPGDDKGRLPVSNVKETSDRTSVSQVLTSRSKESGFSPVADEPHKGGPEQTSPSAVSLSSPAKTKILPPRKGRGLKLEAIVQKITSPNVRRISAPSSSESAAEAVTLDDILSLKIKGPESGKGSMQEAELPGVVGPDISCSEETTAKSLPPKSLDVWAHDEKKIKTEMEEQSMETKDFSMSGLPAQASNHYMDGKGTSTALEPKCVLPQVQQHENVEKEKLAGTPCNVTPKQEVIPQKGYFPSGKKKGRPVGSVNKQKKQQQQPLMQEQGHQQPPPPVSPASVSALPPQHLDEHLDISEAEPKPKRRRTERRKPTGAPRRRRGKQAAPIVAPIEPEIKLKYASQNLDKPETKTKSFIPYIHVENKEELGLSCVIINAEEEEQHWKKLTSVRKGQRSASPQPSTDSKALPSSSFMVQGPVVTESATMGHLVCCLCGKWANYRNLGDLFGPFYNQEYASTLSKNPPPKKSSETPRKVKVRHKDTSDGSKSDSDEEEEEEPQQAREQRSLTAHPRYKRRNRSGDCTSSRLTVPSHRKTTDPSELTPMDSSGPSTAEAGPEQGVQIPQLPLDSNEFWMHEGCVLWANGVYFVCGRLYGLREAVDIAREMKCSHCQETGATLGCYNKGCACCYHFPCAMDSECLLNEENFSVRCPKHKTKTVKGVSSEQPEQG

Target ORF information:

RefSeq Version	XM_031901057.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901057.1

ORF Insert Sequence:

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCACAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGTT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGACCAAGAC AGTAAAGGGT 
GTTTCATCAG AGCAGCCTGA GCAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcf20 ( XM_002934712.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934758.2 Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X4, mRNA.

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CloneID	OXa57368
Clone ID Related Accession (Same CDS sequence)	XM_002934712.5
Accession Version	XM_002934712.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934712.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCACAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGTT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGGTAAAGCT TTTTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934758.2
CDS	196..5715
Translation	MQSFREQSSYGSQQTYPPEAHGPSRLEEFSPRQQAQIFQGNYGGRRGGSSTPATIPAGETSSIQSYQSYRKESGDFYYLGNKEGGTPASQTPQRRPSGPVQSYGPPQANSSSGTNFGTPYGREGHHSQFANQHSAAGNLSQYSQDFPSSFSPGGSQYPSHVTSQQLRQQLYQSHQSLSQSGNPPTSAGASHLQQIQRSTALNSPAGYQLRMGQYGQHYQPSPGSSSSSFPSSQRFGQTGPNYEGYSPGGASSTYESHMPGSSYGTQQGFSSYASQHMKSFETSKLPQNVGQGQSQQQQQTSHVMQYSNSSKLPLQSQTGQYSQSEVPVRSPMQFQQNFSPISNPSPAASVVQSPSCSSTPSPLMTSGENLTCGQGNVPLSSRNRILQMMPQLSPTPHGGGFKGFGVEGPPDKRLTDPGLSSLSALSSQVANLPNTVQHMLLSDALAPNKKSGKRTSRKADSCANSETSSQAEEQLKSPMAESLDGSCSSSSGDPGERVRQLSGQSTSSEAGYKGTSSEKPASPTQGWSTELSEKTQAEEVETVPEATETFQEVSKVSEKSVGVIVAMETVATRSEKVTEESSTLDNDEVETSSQPEVPETVKDDDNVKECPPDQNGEPSDADSQPVPITSSTSRTEAKSPVGSGFSFKEGATSSRNASNLPQHLKTPDSAGCGGQGDKKSGTGRNDKYPSLLQEVLQGHHQQERRYARNTQDPPPVTGSSEASLRPNILMSQASELPNRNILGKTLAPHLETPNWGPWDRKSAPDVKPLNVTDYSARKFEVDPPASSQESSGIPSERRSVICDISPLRQLVRDPTASHHVGQERSADGRSGQSVILPSGMISLDAKSSNQGGTPKEQDTEATKMHRKRTTDHCIPYSTKESARGNGSPRSMTFDPTQDYSPHNSRGPHPRRGTARVGARGRSPVQDLGDKMKMSPGRSRGPAETHHMNPTMSLSERASREAFYSAFFQNADNAALSYHSNSRASSYGEPHPTFTSPLHYKRPVYQQEEFKEWLGSSILSASQHRQEMQRKSPRQEQFHVRSPGRVENEVMVYNQPGAYHDSSSLEYGRQLRVGEGGPTNLTALELKRNAQKTPPVDSTNWNLGRQTSPVKKLGSPVGTNQKPFSSGRETDTLVHRSGEGTEISKPGCNAHRPQGTEEQSHHNPLIMRRRVRSFISPIPSKRQPGDDKGRLPVSNVKETSDRTSVSQVLTSRSKESGFSPVADEPHKGGPEQTSPSAVSLSSPAKTKILPPRKGRGLKLEAIVQKITSPNVRRISAPSSSESAAEAVTLDDILSLKIKGPESGKGSMQEAELPGVVGPDISCSEETTAKSLPPKSLDVWAHDEKKIKTEMEEQSMETKDFSMSGLPAQASNHYMDGKGTSTALEPKCVLPQVQQHENVEKEKLAGTPCNVTPKQEVIPQKGYFPSGKKKGRPVGSVNKQKKQQQQPLMQEQGHQQPPPPVSPASVSALPPQHLDEHLDISEAEPKPKRRRTERRKPTGAPRRRRGKQAAPIVAPIEPEIKLKYASQNLDKPETKTKSFIPYIHVENKEELGLSCVIINAEEEEQHWKKLTSVRKGQRSASPQPSTDSKALPSSSFMVQGPVVTESATMGHLVCCLCGKWANYRNLGDLFGPFYNQEYASTLSKNPPPKKSSETPRKVKVRHKDTSDGSKSDSDEEEEEEPQQAREQRSLTAHPRYKRRNRSGDCTSSRLTVPSHRKTTDPSELTPMDSSGPSTAEAGPEQGVQIPQLPLDSNEFWMHEGCVLWANGVYFVCGRLYGLREAVDIAREMKCSHCQETGATLGCYNKGCACCYHFPCAMDSECLLNEENFSVRCPKHKVKLFR

Target ORF information:

RefSeq Version	XM_002934712.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934712.5

ORF Insert Sequence:

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCACAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGTT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGGTAAAGCT TTTTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcf20 ( XM_004913793.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913850.1 Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X1, mRNA.

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CloneID	OXa09201
Clone ID Related Accession (Same CDS sequence)	XM_004913793.3 , XM_002934714.4
Accession Version	XM_004913793.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913793.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCGCAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGCT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGACCAAGAC AGTAAAGGGT 
GTTTCATCAG AGCAGCCTGA GCAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913850.1
CDS	216..5765
Translation	MQSFREQSSYGSQQTYPPEAHGPSRLEEFSPRQQAQIFQGNYGGRRGGSSTPATIPAGETSSIQSYQSYRKESGDFYYLGNKEGGTPASQTPQRRPSGPVQSYGPPQANSSSGTNFGTPYGREGHHSQFANQHSAAGNLSQYSQDFPSSFSPGGSQYPSHVTSQQLRQQLYQSHQSLSQSGNPPTSAGASHLQQIQRSTALNSPAGYQLRMGQYGQHYQPSPGSSSSSFPSSQRFGQTGPNYEGYSPGGASSTYESHMPGSSYGTQQGFSSYASQHMKSFETSKLPQNVGQGQSQQQQQTSHVMQYSNSSKLPLQSQTGQYSQSEVPVRSPMQFQQNFSPISNPSPAASVVQSPSCSSTPSPLMTSGENLTCGQGNVPLSSRNRILQMMPQLSPTPHGGGFKGFGVEGPPDKRLTDPGLSSLSALSSQVANLPNTVQHMLLSDALAPNKKSGKRTSRKADSCANSETSSQAEEQLKSPMAESLDGSCSSSSGDPGERVRQLSGQSTSSEAGYKGTSSEKPASPTQGWSTELSEKTQAEEVETVPEATETFQEVSKVSEKSVGVIVAMETVATRSEKVTEESSTLDNDEVETSSQPEVPETVKDDDNVKECPPDQNGEPSDADSQPVPITSSTSRTEAKSPVGSGFSFKEGATSSRNASNLPQHLKTPDSAGCGGQGDKKSGTGRNDKYPSLLQEVLQGHHQQERRYARNTQDPPPVTGSSEASLRPNILMSQASELPNRNILGKTLAPHLETPNWGPWDRKSAPDVKPLNVTDYSARKFEVDPPASSQESSGIPSERRSVICDISPLRQLVRDPTASHHVGQERSADGRSGQSVILPSGMISLDAKSSNQGGTPKEQDTEATKMHRKRTTDHCIPYSTKESARGNGSPRSMTFDPTQDYSPHNSRGPHPRRGTARVGARGRSPVQDLGDKMKMSPGRSRGPAETHHMNPTMSLSERASREAFYSAFFQNADNAALSYHSNSRASSYGEPHPTFTSPLHYKRPVYQQEEFKEWLGSSILSASQHRQEMQRKSPRQEQFHVRSPGRVENEVMVYNQPGAYHDSSSLEYGRQLRVGEGGPTNLTALELKRNAQKTPPVDSTNWNLGRQTSPVKKLGSPVGTNQKPFSSGRETDTLVHRSGEGTEISKPGCNAHRPQGTEEQSHHNPLIMRRRVRSFISPIPSKRQPGDDKGRLPVSNVKETSDRTSVSQVLTSRSKESGFSPVADEPHKGGPEQTSPSAVSLSSPAKTKILPPRKGRGLKLEAIVQKITSPNVRRISAPSSSESAAEAVTLDDILSLKIKGPESGKGSMQEAELPGVVGPDISCSEETTAKSLPPKSLDVWAHDEKKIKTEMEEQSMETKDFSMSGLPAQASNHYMDGKGTSTALEPKCVLPQVQQHENVEKEKLAGTPCNVTPKQEVIPQKGYFPSGKKKGRPVGSVNKQKKQQQQPLMQEQGHQQPPPPVSPASVSALPPQHLDEHLDISEAEPKPKRRRTERRKPTGAPRRRRGKQAAPIVAPIEPEIKLKYASQNLDKPETKTKSFIPYIHVENKEELGLSCVIINAEEEEQHWKKLTSVRKGQRSASPQPSTDSKALPSSSFMVQGPAVTESATMGHLVCCLCGKWANYRNLGDLFGPFYNQEYASTLSKNPPPKKSSETPRKVKVRHKDTSDGSKSDSDEEEEEEPQQAREQRSLTAHPRYKRRNRSGDCTSSRLTVPSHRKTTDPSELTPMDSSGPSTAEAGPEQGVQIPQLPLDSNEFWMHEGCVLWANGVYFVCGRLYGLREAVDIAREMKCSHCQETGATLGCYNKGCACCYHFPCAMDSECLLNEENFSVRCPKHKTKTVKGVSSEQPEQG

Target ORF information:

RefSeq Version	XM_004913793.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913793.3

ORF Insert Sequence:

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCGCAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGCT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGACCAAGAC AGTAAAGGGT 
GTTTCATCAG AGCAGCCTGA GCAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcf20 ( XM_002934714.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934760.1 Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X2, mRNA.

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CloneID	OXa09201
Clone ID Related Accession (Same CDS sequence)	XM_004913793.3 , XM_002934714.4
Accession Version	XM_002934714.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934714.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCGCAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGCT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGACCAAGAC AGTAAAGGGT 
GTTTCATCAG AGCAGCCTGA GCAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934760.1
CDS	326..5875
Translation	MQSFREQSSYGSQQTYPPEAHGPSRLEEFSPRQQAQIFQGNYGGRRGGSSTPATIPAGETSSIQSYQSYRKESGDFYYLGNKEGGTPASQTPQRRPSGPVQSYGPPQANSSSGTNFGTPYGREGHHSQFANQHSAAGNLSQYSQDFPSSFSPGGSQYPSHVTSQQLRQQLYQSHQSLSQSGNPPTSAGASHLQQIQRSTALNSPAGYQLRMGQYGQHYQPSPGSSSSSFPSSQRFGQTGPNYEGYSPGGASSTYESHMPGSSYGTQQGFSSYASQHMKSFETSKLPQNVGQGQSQQQQQTSHVMQYSNSSKLPLQSQTGQYSQSEVPVRSPMQFQQNFSPISNPSPAASVVQSPSCSSTPSPLMTSGENLTCGQGNVPLSSRNRILQMMPQLSPTPHGGGFKGFGVEGPPDKRLTDPGLSSLSALSSQVANLPNTVQHMLLSDALAPNKKSGKRTSRKADSCANSETSSQAEEQLKSPMAESLDGSCSSSSGDPGERVRQLSGQSTSSEAGYKGTSSEKPASPTQGWSTELSEKTQAEEVETVPEATETFQEVSKVSEKSVGVIVAMETVATRSEKVTEESSTLDNDEVETSSQPEVPETVKDDDNVKECPPDQNGEPSDADSQPVPITSSTSRTEAKSPVGSGFSFKEGATSSRNASNLPQHLKTPDSAGCGGQGDKKSGTGRNDKYPSLLQEVLQGHHQQERRYARNTQDPPPVTGSSEASLRPNILMSQASELPNRNILGKTLAPHLETPNWGPWDRKSAPDVKPLNVTDYSARKFEVDPPASSQESSGIPSERRSVICDISPLRQLVRDPTASHHVGQERSADGRSGQSVILPSGMISLDAKSSNQGGTPKEQDTEATKMHRKRTTDHCIPYSTKESARGNGSPRSMTFDPTQDYSPHNSRGPHPRRGTARVGARGRSPVQDLGDKMKMSPGRSRGPAETHHMNPTMSLSERASREAFYSAFFQNADNAALSYHSNSRASSYGEPHPTFTSPLHYKRPVYQQEEFKEWLGSSILSASQHRQEMQRKSPRQEQFHVRSPGRVENEVMVYNQPGAYHDSSSLEYGRQLRVGEGGPTNLTALELKRNAQKTPPVDSTNWNLGRQTSPVKKLGSPVGTNQKPFSSGRETDTLVHRSGEGTEISKPGCNAHRPQGTEEQSHHNPLIMRRRVRSFISPIPSKRQPGDDKGRLPVSNVKETSDRTSVSQVLTSRSKESGFSPVADEPHKGGPEQTSPSAVSLSSPAKTKILPPRKGRGLKLEAIVQKITSPNVRRISAPSSSESAAEAVTLDDILSLKIKGPESGKGSMQEAELPGVVGPDISCSEETTAKSLPPKSLDVWAHDEKKIKTEMEEQSMETKDFSMSGLPAQASNHYMDGKGTSTALEPKCVLPQVQQHENVEKEKLAGTPCNVTPKQEVIPQKGYFPSGKKKGRPVGSVNKQKKQQQQPLMQEQGHQQPPPPVSPASVSALPPQHLDEHLDISEAEPKPKRRRTERRKPTGAPRRRRGKQAAPIVAPIEPEIKLKYASQNLDKPETKTKSFIPYIHVENKEELGLSCVIINAEEEEQHWKKLTSVRKGQRSASPQPSTDSKALPSSSFMVQGPAVTESATMGHLVCCLCGKWANYRNLGDLFGPFYNQEYASTLSKNPPPKKSSETPRKVKVRHKDTSDGSKSDSDEEEEEEPQQAREQRSLTAHPRYKRRNRSGDCTSSRLTVPSHRKTTDPSELTPMDSSGPSTAEAGPEQGVQIPQLPLDSNEFWMHEGCVLWANGVYFVCGRLYGLREAVDIAREMKCSHCQETGATLGCYNKGCACCYHFPCAMDSECLLNEENFSVRCPKHKTKTVKGVSSEQPEQG

Target ORF information:

RefSeq Version	XM_002934714.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934714.4

ORF Insert Sequence:

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCGCAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGCT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGACCAAGAC AGTAAAGGGT 
GTTTCATCAG AGCAGCCTGA GCAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tcf20 ( XM_002934712.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934758.1 Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X3, mRNA.

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CloneID	OXa09202
Clone ID Related Accession (Same CDS sequence)	XM_002934712.4
Accession Version	XM_002934712.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription factor 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934712.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCGCAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGCT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGGTAAAGCT TTTTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934758.1
CDS	214..5733
Translation	MQSFREQSSYGSQQTYPPEAHGPSRLEEFSPRQQAQIFQGNYGGRRGGSSTPATIPAGETSSIQSYQSYRKESGDFYYLGNKEGGTPASQTPQRRPSGPVQSYGPPQANSSSGTNFGTPYGREGHHSQFANQHSAAGNLSQYSQDFPSSFSPGGSQYPSHVTSQQLRQQLYQSHQSLSQSGNPPTSAGASHLQQIQRSTALNSPAGYQLRMGQYGQHYQPSPGSSSSSFPSSQRFGQTGPNYEGYSPGGASSTYESHMPGSSYGTQQGFSSYASQHMKSFETSKLPQNVGQGQSQQQQQTSHVMQYSNSSKLPLQSQTGQYSQSEVPVRSPMQFQQNFSPISNPSPAASVVQSPSCSSTPSPLMTSGENLTCGQGNVPLSSRNRILQMMPQLSPTPHGGGFKGFGVEGPPDKRLTDPGLSSLSALSSQVANLPNTVQHMLLSDALAPNKKSGKRTSRKADSCANSETSSQAEEQLKSPMAESLDGSCSSSSGDPGERVRQLSGQSTSSEAGYKGTSSEKPASPTQGWSTELSEKTQAEEVETVPEATETFQEVSKVSEKSVGVIVAMETVATRSEKVTEESSTLDNDEVETSSQPEVPETVKDDDNVKECPPDQNGEPSDADSQPVPITSSTSRTEAKSPVGSGFSFKEGATSSRNASNLPQHLKTPDSAGCGGQGDKKSGTGRNDKYPSLLQEVLQGHHQQERRYARNTQDPPPVTGSSEASLRPNILMSQASELPNRNILGKTLAPHLETPNWGPWDRKSAPDVKPLNVTDYSARKFEVDPPASSQESSGIPSERRSVICDISPLRQLVRDPTASHHVGQERSADGRSGQSVILPSGMISLDAKSSNQGGTPKEQDTEATKMHRKRTTDHCIPYSTKESARGNGSPRSMTFDPTQDYSPHNSRGPHPRRGTARVGARGRSPVQDLGDKMKMSPGRSRGPAETHHMNPTMSLSERASREAFYSAFFQNADNAALSYHSNSRASSYGEPHPTFTSPLHYKRPVYQQEEFKEWLGSSILSASQHRQEMQRKSPRQEQFHVRSPGRVENEVMVYNQPGAYHDSSSLEYGRQLRVGEGGPTNLTALELKRNAQKTPPVDSTNWNLGRQTSPVKKLGSPVGTNQKPFSSGRETDTLVHRSGEGTEISKPGCNAHRPQGTEEQSHHNPLIMRRRVRSFISPIPSKRQPGDDKGRLPVSNVKETSDRTSVSQVLTSRSKESGFSPVADEPHKGGPEQTSPSAVSLSSPAKTKILPPRKGRGLKLEAIVQKITSPNVRRISAPSSSESAAEAVTLDDILSLKIKGPESGKGSMQEAELPGVVGPDISCSEETTAKSLPPKSLDVWAHDEKKIKTEMEEQSMETKDFSMSGLPAQASNHYMDGKGTSTALEPKCVLPQVQQHENVEKEKLAGTPCNVTPKQEVIPQKGYFPSGKKKGRPVGSVNKQKKQQQQPLMQEQGHQQPPPPVSPASVSALPPQHLDEHLDISEAEPKPKRRRTERRKPTGAPRRRRGKQAAPIVAPIEPEIKLKYASQNLDKPETKTKSFIPYIHVENKEELGLSCVIINAEEEEQHWKKLTSVRKGQRSASPQPSTDSKALPSSSFMVQGPAVTESATMGHLVCCLCGKWANYRNLGDLFGPFYNQEYASTLSKNPPPKKSSETPRKVKVRHKDTSDGSKSDSDEEEEEEPQQAREQRSLTAHPRYKRRNRSGDCTSSRLTVPSHRKTTDPSELTPMDSSGPSTAEAGPEQGVQIPQLPLDSNEFWMHEGCVLWANGVYFVCGRLYGLREAVDIAREMKCSHCQETGATLGCYNKGCACCYHFPCAMDSECLLNEENFSVRCPKHKVKLFR

Target ORF information:

RefSeq Version	XM_002934712.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transcription factor 20 (AR1) (tcf20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934712.4

ORF Insert Sequence:

ATGCAGTCCT TTCGAGAGCA AAGCAGTTAT GGCAGCCAGC AGACTTATCC TCCGGAAGCT 
CATGGCCCCT CCAGGCTGGA AGAGTTTAGC CCAAGACAGC AGGCTCAGAT ATTCCAGGGA 
AACTATGGAG GACGTCGAGG AGGGTCGAGC ACTCCTGCCA CAATTCCAGC TGGAGAAACT 
TCAAGTATTC AGAGCTACCA GTCTTACAGA AAAGAATCTG GGGATTTTTA TTATTTGGGT 
AACAAAGAAG GAGGTACACC TGCAAGCCAG ACACCGCAGC GGAGGCCATC AGGGCCAGTG 
CAAAGCTATG GACCTCCTCA AGCAAACAGC AGCAGTGGTA CAAACTTTGG CACACCCTAT 
GGAAGGGAGG GGCATCACAG CCAATTTGCG AACCAGCATT CAGCAGCAGG CAATCTCTCC 
CAGTACTCGC AGGACTTTCC AAGTTCGTTT TCTCCTGGTG GTAGTCAGTA CCCTTCTCAT 
GTCACTAGCC AGCAGTTGAG GCAGCAGCTT TATCAATCAC ACCAATCCCT GTCCCAGTCT 
GGGAACCCAC CAACATCTGC AGGTGCCTCA CATCTACAGC AGATACAGCG ATCTACTGCA 
CTCAACTCCC CTGCTGGATA CCAGTTAAGA ATGGGGCAGT ATGGTCAACA CTACCAGCCT 
TCTCCTGGCT CTTCTTCTAG TTCTTTTCCT TCTTCTCAGA GGTTTGGACA GACTGGTCCA 
AACTATGAGG GTTACAGTCC AGGAGGGGCT AGCTCTACAT ATGAGAGCCA TATGCCAGGG 
TCATCATATG GAACACAGCA AGGGTTTAGC AGTTATGCCA GTCAGCACAT GAAAAGCTTT 
GAGACATCTA AATTGCCACA GAATGTAGGG CAGGGACAGT CACAACAACA GCAACAGACT 
TCTCATGTCA TGCAGTACTC CAATTCTTCT AAACTACCCC TACAAAGCCA AACAGGACAA 
TACAGTCAGT CTGAAGTCCC AGTACGCTCT CCTATGCAGT TCCAACAAAA TTTCAGTCCA 
ATCTCAAATC CTTCCCCTGC AGCCTCAGTT GTACAGTCCC CAAGCTGTAG CTCCACTCCC 
TCACCTTTGA TGACAAGTGG AGAAAACCTC ACATGTGGGC AGGGTAATGT GCCTCTGTCA 
TCCAGAAACC GTATTTTGCA GATGATGCCA CAGCTAAGCC CTACACCTCA CGGGGGAGGC 
TTTAAAGGGT TTGGTGTTGA AGGTCCTCCT GACAAACGAC TGACTGACCC AGGTCTAAGC 
AGCCTTAGTG CTCTAAGCAG TCAGGTTGCA AACCTGCCCA ACACAGTTCA GCATATGCTG 
CTTTCGGATG CTCTGGCACC TAACAAGAAA AGTGGGAAGC GAACATCTAG AAAGGCTGAT 
AGCTGTGCCA ACTCAGAAAC CTCTTCCCAA GCTGAAGAAC AGCTGAAATC TCCAATGGCA 
GAGTCACTAG ATGGCAGCTG TTCCAGCAGT TCAGGGGATC CTGGTGAAAG GGTAAGGCAA 
CTTAGCGGGC AAAGCACAAG CTCTGAAGCT GGTTACAAGG GAACTTCTTC TGAAAAGCCA 
GCTTCTCCTA CACAGGGTTG GTCAACTGAG CTTTCTGAAA AAACACAAGC GGAAGAAGTG 
GAAACTGTTC CAGAAGCCAC AGAGACTTTT CAGGAAGTGT CCAAGGTCAG TGAGAAATCT 
GTGGGTGTCA TAGTAGCAAT GGAGACTGTA GCCACCAGGT CAGAGAAAGT CACAGAAGAA 
TCTTCAACAC TAGATAATGA TGAAGTTGAA ACGTCATCAC AGCCAGAGGT GCCTGAAACA 
GTTAAAGATG ATGATAATGT CAAGGAATGC CCCCCTGACC AGAATGGAGA ACCTAGTGAT 
GCCGATTCTC AGCCTGTTCC TATTACAAGT AGTACTTCTA GAACAGAGGC TAAATCTCCT 
GTGGGCTCTG GGTTTAGTTT TAAAGAAGGA GCAACAAGCT CTAGAAATGC CAGCAACTTG 
CCTCAGCATC TTAAAACTCC TGATTCAGCA GGGTGTGGAG GACAGGGTGA TAAGAAATCA 
GGAACAGGAA GAAATGATAA GTATCCTAGC CTCCTTCAGG AAGTCCTGCA AGGACATCAT 
CAGCAGGAAC GAAGGTATGC TAGAAACACC CAAGACCCTC CACCAGTTAC TGGAAGTTCT 
GAAGCAAGTT TGAGACCAAA TATACTCATG AGCCAGGCAA GTGAGTTACC TAACCGTAAC 
ATCCTTGGCA AGACACTTGC TCCACATTTA GAAACGCCTA ACTGGGGCCC ATGGGATAGA 
AAGTCAGCTC CTGATGTTAA ACCATTGAAT GTAACAGATT ATTCTGCAAG AAAATTTGAG 
GTGGACCCTC CAGCCTCCTC TCAGGAATCT AGTGGCATCC CTTCAGAAAG ACGGTCTGTA 
ATATGTGATA TCTCTCCACT GAGGCAGCTT GTTCGAGATC CTACTGCTTC TCATCATGTT 
GGACAAGAAA GATCAGCTGA TGGAAGATCT GGACAGTCTG TAATCTTACC TAGTGGAATG 
ATATCTTTAG ATGCAAAGTC AAGTAACCAG GGAGGAACGC CTAAAGAACA AGACACTGAA 
GCAACAAAGA TGCATAGAAA AAGAACTACA GATCACTGCA TACCATACAG CACCAAAGAA 
TCTGCTAGGG GCAATGGCAG CCCAAGATCC ATGACCTTTG ACCCAACCCA GGATTATAGT 
CCACATAATA GTAGAGGACC TCATCCCAGA AGGGGAACTG CCAGAGTGGG GGCCCGTGGT 
AGATCACCTG TACAGGATCT TGGTGACAAA ATGAAGATGT CTCCAGGGAG AAGCAGAGGA 
CCAGCAGAAA CTCATCATAT GAATCCAACA ATGAGCCTCT CAGAAAGAGC AAGCAGAGAA 
GCTTTTTACT CTGCTTTTTT TCAAAATGCT GATAATGCTG CTTTGAGTTA TCATTCAAAC 
TCTAGAGCAA GCTCCTATGG AGAGCCTCAC CCAACGTTTA CATCTCCTCT TCATTATAAG 
AGACCAGTGT ACCAGCAGGA GGAATTTAAA GAATGGCTGG GAAGCTCTAT ACTTTCTGCA 
TCACAGCATC GTCAAGAAAT GCAGCGTAAA AGCCCAAGGC AGGAGCAGTT TCATGTCCGT 
AGTCCAGGAA GGGTTGAGAA TGAGGTAATG GTTTACAACC AGCCAGGTGC ATATCATGAT 
TCTAGCAGTC TAGAATATGG ACGCCAGCTG CGTGTTGGTG AGGGAGGACC CACCAATTTG 
ACAGCATTGG AGCTTAAAAG AAATGCTCAA AAAACACCTC CAGTGGACTC TACTAACTGG 
AATTTGGGCC GTCAGACTTC TCCTGTCAAA AAACTTGGGT CCCCTGTAGG GACAAACCAA 
AAACCCTTTA GTTCTGGTAG GGAAACAGAT ACTCTTGTCC ATAGATCAGG AGAAGGAACT 
GAGATCTCCA AGCCCGGGTG CAATGCCCAT AGACCTCAAG GCACAGAAGA ACAATCACAC 
CATAACCCTC TAATCATGAG AAGAAGAGTA CGTTCTTTTA TCTCTCCAAT TCCCAGCAAA 
AGGCAGCCTG GTGATGACAA AGGGCGCCTG CCTGTTTCTA ATGTAAAGGA AACTTCAGAC 
AGGACATCTG TGTCACAGGT ATTAACTTCT AGGAGCAAAG AAAGTGGCTT TTCCCCTGTG 
GCAGATGAAC CCCACAAAGG TGGCCCAGAA CAGACAAGCC CATCAGCTGT ATCCCTTTCT 
AGTCCAGCCA AGACAAAAAT CCTTCCTCCC CGCAAAGGGA GGGGTCTAAA ACTGGAGGCA 
ATTGTACAGA AAATCACTTC ACCTAATGTT CGTCGTATTT CAGCCCCAAG TAGCTCAGAA 
AGCGCAGCTG AAGCTGTAAC ACTAGATGAT ATTCTCTCTC TAAAAATCAA GGGCCCAGAG 
AGTGGAAAAG GTTCCATGCA AGAAGCAGAA TTGCCTGGGG TTGTTGGACC AGATATCTCC 
TGTAGTGAAG AGACAACTGC AAAAAGCCTG CCTCCCAAGT CACTTGATGT ATGGGCACAT 
GATGAAAAGA AAATCAAAAC TGAAATGGAG GAGCAGTCTA TGGAAACTAA AGACTTTTCA 
ATGTCTGGGC TTCCAGCACA AGCGTCTAAC CATTATATGG ATGGAAAAGG TACATCTACG 
GCCTTGGAAC CAAAATGTGT TTTACCCCAG GTGCAGCAGC ATGAAAATGT AGAAAAGGAG 
AAACTTGCAG GAACACCTTG TAATGTTACT CCAAAACAAG AAGTCATACC TCAGAAGGGA 
TATTTCCCCT CTGGAAAGAA GAAAGGACGT CCTGTTGGTA GTGTCAACAA ACAAAAGAAA 
CAGCAGCAAC AGCCATTGAT GCAAGAGCAA GGGCATCAGC AGCCACCACC ACCTGTTTCA 
CCAGCCTCAG TCTCTGCTCT CCCACCACAG CATTTGGATG AGCATTTGGA TATCTCTGAG 
GCAGAGCCTA AACCTAAAAG GAGGAGGACA GAAAGAAGAA AGCCTACTGG AGCACCAAGA 
AGAAGAAGGG GAAAACAAGC TGCTCCTATA GTTGCCCCTA TAGAGCCAGA AATCAAACTT 
AAATATGCCA GCCAAAATTT AGACAAGCCT GAGACCAAAA CCAAGTCTTT TATTCCTTAT 
ATACATGTGG AAAATAAAGA GGAGCTGGGA TTGTCTTGTG TCATCATCAA TGCTGAAGAG 
GAGGAACAAC ATTGGAAGAA ATTAACATCA GTTCGCAAAG GTCAGCGATC TGCTTCTCCA 
CAACCCAGCA CAGACAGTAA AGCACTTCCT TCATCTAGCT TTATGGTACA AGGGCCAGCT 
GTCACAGAGT CTGCTACCAT GGGCCACCTT GTCTGTTGCC TTTGTGGTAA GTGGGCTAAT 
TACAGGAATT TGGGAGATTT GTTTGGCCCA TTCTACAACC AAGAATATGC ATCTACGCTT 
TCTAAGAATC CCCCACCAAA AAAATCATCA GAGACTCCTC GAAAAGTTAA AGTTCGTCAC 
AAAGACACCT CTGATGGTTC TAAGTCTGAC TCTGATGAGG AAGAGGAAGA AGAACCACAG 
CAAGCACGAG AACAACGTAG TCTAACTGCT CATCCTCGCT ACAAACGTCG GAACCGTTCA 
GGAGATTGTA CCTCCTCCAG ACTTACTGTA CCTTCCCACA GGAAGACCAC TGACCCTTCT 
GAACTAACTC CCATGGACTC TAGTGGGCCA TCCACAGCTG AGGCAGGCCC TGAGCAAGGG 
GTTCAGATCC CCCAACTACC TCTCGACAGC AATGAATTTT GGATGCACGA GGGGTGTGTC 
TTATGGGCCA ATGGTGTCTA CTTTGTCTGT GGCCGATTAT ATGGTCTTCG GGAGGCTGTT 
GACATTGCAC GAGAGATGAA ATGTAGTCAC TGCCAGGAGA CTGGAGCCAC ATTGGGCTGC 
TACAACAAGG GTTGTGCCTG CTGCTATCAC TTCCCATGTG CCATGGACTC AGAATGTTTA 
CTAAATGAGG AGAACTTCTC TGTACGCTGC CCCAAGCACA AGGTAAAGCT TTTTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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tcf20 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

サポート

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