chsy1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	chsy1
Entrez Gene ID	100036707
Full Name	chondroitin sulfate synthase 1
General protein information	Preferred Names chondroitin sulfate synthase 1 Names chondroitin sulfate synthase 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031897498.1	XP_031753358.1	chondroitin sulfate synthase 1 isoform X1
NM_001097255.1	NP_001090724.1	chondroitin sulfate synthase 1 precursor

Pathways from BioSystems

Homologies

	CHSY1	XP_001084554.2
Rattus norvegicus (Norway rat)	Chsy1	NP_001099738.1
Homo sapiens (human)	CHSY1	NP_055733.2
Gallus gallus (chicken)	CHSY1	XP_413889.3
Drosophila melanogaster (fruit fly)	CG9220	NP_996440.2
Canis lupus familiaris (dog)	CHSY1	XP_545821.3
Danio rerio (zebrafish)	chsy1	NP_997843.1
Bos taurus (cattle)	CHSY1	NP_001178086.1
Mus musculus (house mouse)	Chsy1	NP_001074632.1
Xenopus tropicalis (tropical clawed frog)	chsy1	NP_001090724.1
Pan troglodytes (chimpanzee)	CHSY1	XP_523172.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001010	XP_309203.5

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chsy1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chsy1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa23508	NM_001097255.1 Latest version!	Xenopus tropicalis chondroitin sulfate synthase 1 (chsy1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa53123	XM_031897498.1 Latest version!	Xenopus tropicalis chondroitin sulfate synthase 1 (chsy1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chsy1 ( NM_001097255.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001090724.1 Xenopus tropicalis chondroitin sulfate synthase 1 (chsy1), mRNA.

Price & Availability↑

CloneID	OXa23508
Clone ID Related Accession (Same CDS sequence)	NM_001097255.1
Accession Version	NM_001097255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chondroitin sulfate synthase 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC127293.1. ##Evidence-Data-START## Transcript exon combination :: BC127293.1, CX396332.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028292, SAMN00862003 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCCA GAAGTCGCCA CTCGTTGCTC AGTGTCCTGC TGGGGCTGGT CCTGGGCTTC 
CTCCTCGCCT CCAGGTTTAT CTTGCCCAGA GCGGCCGAGC TGCAGAGAGC CAGCCGTAGG 
AGAGCGGGCG GTGGGGGGGT ATCAGGGGGG CAGGCTGGGA GCTGTGGCCG ACAATCTGCC 
TTAAGGAACG ATTACAGCGG GGTGCTGGGC TGGCAACAGC GGCTACACAC GGACACCGGG 
GCTCTGAGCC CGGCCAGCGA GGAAGACATC CCCACGGACA AGAACTTCCT TTTTGTTGGG 
GTCATGACAG CTCAGAAGTA CCTGGAAACC AGAGCAGTGG CCGCTTATGG GACTTGGGCA 
AACTCCATTC CTGGTAAAGT TGAATTTTTC TCCAGTGAAG GCTCAGATAC ATCTATACCA 
ATTCCAATTG TGCCACTGCA AGGTGTGGAT GACTCCTATC CACCACAGAA GAAGTCCTTC 
ATGATGCTCA AGTACATGCA TGATCACTAC TTGGATCAAT ATGAGTGGTT TATGCGAGCA 
GATGATGATG TTTACATCAA AGGGGATCGC TTGGAGAACT TTTTGAGGAG CCTCAACAGC 
AGCGAACCAC TGTTCCTTGG CCAGACTGGC CTTGGCACCA CAGAGGAAAT GGGCAAATTG 
GCACTGGAGC CCGGAGAGAA TTTCTGTATG GGTGGCCCTG GTGTGATCAT GAGCAGAGAA 
GTCCTTCGTA GGGTGGTGCC CCACATTGGC GAGTGTCTAA GGCAGATGTT TACCACCCAT 
GAGGATGTGG AGATTGGCAG ATGTGTCAGA AGATTTGCCG GAGTGCAGTG TGTCTGGTCC 
TATGAGATGC AGCAGTTGTT CTATGAGAAT TATGAACAGA ACAAGAAGGG CTACATCCGA 
GATCTTCATA ACAGCAAAAT TCACAGAGCC ATCACTTTAC ATCCCAATAA AAACCCTCCC 
TACCAGTACC GCTTGCACAG CTACATGCTG AGTCGCAAAA TAGCAGAGCT CCGGTATCGT 
ACGATCCAGC TGCACCGGGA GATTGTTCTT ATGAGCAAGT ACAGTAATGC AGAAATCCAT 
AAAGATGATA TGCAACTGGG GATTGCTCCA TCTTTCATGC GGTTCCAACC ACGCCAAAGA 
GAGGAGATTC TGGAGTGGGA ATTTCTCACT GGCAAGTATC TGTATTCAAC TGCTGATGGA 
CAACCTCCTA GACGAGGAAT GGATTCTTCC CAAAAGGAGG CTCTTGATGA CATTGTAATG 
CAAGTTATGG AGATGATAAA TGCCAATGCC AAGGTTCGAG GTCGTATTAT TGACTTTAAA 
GAAATCCAGT ACGGATACCG TAGAGTTAAT CCAATGTATG GTGCAGAATA TGTCTTGGAT 
CTGCTTCTTC TCTACAAAAA GCACAAGGGT AAGAAAATGA CTGTTCCTGT CAGGAGACAT 
GCCTACCTCC AACAGACATT CAGCAAAATT CAGTTCATGG AACATGAGGA GATTGATGCT 
AAAGCGCTGG CGAATAAAAT AAATCAGGAA TCTGGTTCCT TCTACTTTTT GTCAAATTCT 
CTTAAGATGT TTGTTCCCTT TCATCTCACT GCATCGAAGA CTGAACATAA AGAGCCCAAA 
GAAACCAAGG TGAACATCCT GGTTCCCCTC TCTGGCCGAT TTGATATGTT TGCCAGGTTT 
ATGGCCAACT TTGAGAAAAC CTGCCTCATC CCAAATCAAA ATGTGAAGTT AGTCATTCTA 
CTTTTCAATT CTGACTCCAA TCCTGACAAA ACCAGGCAAG TTGAACTCAT GAGAGATTAC 
CGTGTTAAAT ATCCCAAAGC AGATATGCAG ATCTTACCAG TGTCTGGAGA GTTCTCCAGG 
GCTTTGGCTT TAGAAGTTGG ATCCTCGCAG TTTCGAAATG ACTCTCTGCT CTTCTTTTGT 
GATGTGGATT TAGTTTTCTC CACTGAGTTT ATACAACGAT GCAGGGCAAA CACCATTTTA 
GGGCAGCAAA TATACTTTCC AATTATATTT AGCCAATATG ACCCCAAGAT TGTTTATGCT 
GGAAAAGTCC CCAGTGATAA CCATTATGCT TTTACTCAGA AGACTGGATT TTGGAGAAAC 
TATGGCTTTG GCATCACCTG TGTTTATAAG GGAGATCTTT TACGTGCTGG TGGCTTTGAT 
GTTTCCATTC AAGGATGGGG ACTAGAGGAT GTTGACCTTT TCAACAAAGT GGTACAAGTA 
GGTTTAAAGA CCTTTAGGAG TCAGGAGGTT GGAGTCGTTC ACATCCACCA TCCGGTGTTC 
TGTGACCCAA ATCTCGATCC TAAACAGTAC AAAATGTGTT TGGGTTCCAA AGCCTCTACA 
TATGGGTCAA CTCAGCGCCT GGCTGAGTTA TGGCTTGAGA AGAATGACCC AATGTTTGGC 
AGAACCACTC ACACAAATGA ATCTGTGAGG ACAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001090724.1
CDS	515..2953
Translation	MAARSRHSLLSVLLGLVLGFLLASRFILPRAAELQRASRRRAGGGGVSGGQAGSCGRQSALRNDYSGVLGWQQRLHTDTGALSPASEEDIPTDKNFLFVGVMTAQKYLETRAVAAYGTWANSIPGKVEFFSSEGSDTSIPIPIVPLQGVDDSYPPQKKSFMMLKYMHDHYLDQYEWFMRADDDVYIKGDRLENFLRSLNSSEPLFLGQTGLGTTEEMGKLALEPGENFCMGGPGVIMSREVLRRVVPHIGECLRQMFTTHEDVEIGRCVRRFAGVQCVWSYEMQQLFYENYEQNKKGYIRDLHNSKIHRAITLHPNKNPPYQYRLHSYMLSRKIAELRYRTIQLHREIVLMSKYSNAEIHKDDMQLGIAPSFMRFQPRQREEILEWEFLTGKYLYSTADGQPPRRGMDSSQKEALDDIVMQVMEMINANAKVRGRIIDFKEIQYGYRRVNPMYGAEYVLDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKIQFMEHEEIDAKALANKINQESGSFYFLSNSLKMFVPFHLTASKTEHKEPKETKVNILVPLSGRFDMFARFMANFEKTCLIPNQNVKLVILLFNSDSNPDKTRQVELMRDYRVKYPKADMQILPVSGEFSRALALEVGSSQFRNDSLLFFCDVDLVFSTEFIQRCRANTILGQQIYFPIIFSQYDPKIVYAGKVPSDNHYAFTQKTGFWRNYGFGITCVYKGDLLRAGGFDVSIQGWGLEDVDLFNKVVQVGLKTFRSQEVGVVHIHHPVFCDPNLDPKQYKMCLGSKASTYGSTQRLAELWLEKNDPMFGRTTHTNESVRTA

Target ORF information:

RefSeq Version	NM_001097255.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chondroitin sulfate synthase 1 (chsy1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097255.1

ORF Insert Sequence:

ATGGCTGCCA GAAGTCGCCA CTCGTTGCTC AGTGTCCTGC TGGGGCTGGT CCTGGGCTTC 
CTCCTCGCCT CCAGGTTTAT CTTGCCCAGA GCGGCCGAGC TGCAGAGAGC CAGCCGTAGG 
AGAGCGGGCG GTGGGGGGGT ATCAGGGGGG CAGGCTGGGA GCTGTGGCCG ACAATCTGCC 
TTAAGGAACG ATTACAGCGG GGTGCTGGGC TGGCAACAGC GGCTACACAC GGACACCGGG 
GCTCTGAGCC CGGCCAGCGA GGAAGACATC CCCACGGACA AGAACTTCCT TTTTGTTGGG 
GTCATGACAG CTCAGAAGTA CCTGGAAACC AGAGCAGTGG CCGCTTATGG GACTTGGGCA 
AACTCCATTC CTGGTAAAGT TGAATTTTTC TCCAGTGAAG GCTCAGATAC ATCTATACCA 
ATTCCAATTG TGCCACTGCA AGGTGTGGAT GACTCCTATC CACCACAGAA GAAGTCCTTC 
ATGATGCTCA AGTACATGCA TGATCACTAC TTGGATCAAT ATGAGTGGTT TATGCGAGCA 
GATGATGATG TTTACATCAA AGGGGATCGC TTGGAGAACT TTTTGAGGAG CCTCAACAGC 
AGCGAACCAC TGTTCCTTGG CCAGACTGGC CTTGGCACCA CAGAGGAAAT GGGCAAATTG 
GCACTGGAGC CCGGAGAGAA TTTCTGTATG GGTGGCCCTG GTGTGATCAT GAGCAGAGAA 
GTCCTTCGTA GGGTGGTGCC CCACATTGGC GAGTGTCTAA GGCAGATGTT TACCACCCAT 
GAGGATGTGG AGATTGGCAG ATGTGTCAGA AGATTTGCCG GAGTGCAGTG TGTCTGGTCC 
TATGAGATGC AGCAGTTGTT CTATGAGAAT TATGAACAGA ACAAGAAGGG CTACATCCGA 
GATCTTCATA ACAGCAAAAT TCACAGAGCC ATCACTTTAC ATCCCAATAA AAACCCTCCC 
TACCAGTACC GCTTGCACAG CTACATGCTG AGTCGCAAAA TAGCAGAGCT CCGGTATCGT 
ACGATCCAGC TGCACCGGGA GATTGTTCTT ATGAGCAAGT ACAGTAATGC AGAAATCCAT 
AAAGATGATA TGCAACTGGG GATTGCTCCA TCTTTCATGC GGTTCCAACC ACGCCAAAGA 
GAGGAGATTC TGGAGTGGGA ATTTCTCACT GGCAAGTATC TGTATTCAAC TGCTGATGGA 
CAACCTCCTA GACGAGGAAT GGATTCTTCC CAAAAGGAGG CTCTTGATGA CATTGTAATG 
CAAGTTATGG AGATGATAAA TGCCAATGCC AAGGTTCGAG GTCGTATTAT TGACTTTAAA 
GAAATCCAGT ACGGATACCG TAGAGTTAAT CCAATGTATG GTGCAGAATA TGTCTTGGAT 
CTGCTTCTTC TCTACAAAAA GCACAAGGGT AAGAAAATGA CTGTTCCTGT CAGGAGACAT 
GCCTACCTCC AACAGACATT CAGCAAAATT CAGTTCATGG AACATGAGGA GATTGATGCT 
AAAGCGCTGG CGAATAAAAT AAATCAGGAA TCTGGTTCCT TCTACTTTTT GTCAAATTCT 
CTTAAGATGT TTGTTCCCTT TCATCTCACT GCATCGAAGA CTGAACATAA AGAGCCCAAA 
GAAACCAAGG TGAACATCCT GGTTCCCCTC TCTGGCCGAT TTGATATGTT TGCCAGGTTT 
ATGGCCAACT TTGAGAAAAC CTGCCTCATC CCAAATCAAA ATGTGAAGTT AGTCATTCTA 
CTTTTCAATT CTGACTCCAA TCCTGACAAA ACCAGGCAAG TTGAACTCAT GAGAGATTAC 
CGTGTTAAAT ATCCCAAAGC AGATATGCAG ATCTTACCAG TGTCTGGAGA GTTCTCCAGG 
GCTTTGGCTT TAGAAGTTGG ATCCTCGCAG TTTCGAAATG ACTCTCTGCT CTTCTTTTGT 
GATGTGGATT TAGTTTTCTC CACTGAGTTT ATACAACGAT GCAGGGCAAA CACCATTTTA 
GGGCAGCAAA TATACTTTCC AATTATATTT AGCCAATATG ACCCCAAGAT TGTTTATGCT 
GGAAAAGTCC CCAGTGATAA CCATTATGCT TTTACTCAGA AGACTGGATT TTGGAGAAAC 
TATGGCTTTG GCATCACCTG TGTTTATAAG GGAGATCTTT TACGTGCTGG TGGCTTTGAT 
GTTTCCATTC AAGGATGGGG ACTAGAGGAT GTTGACCTTT TCAACAAAGT GGTACAAGTA 
GGTTTAAAGA CCTTTAGGAG TCAGGAGGTT GGAGTCGTTC ACATCCACCA TCCGGTGTTC 
TGTGACCCAA ATCTCGATCC TAAACAGTAC AAAATGTGTT TGGGTTCCAA AGCCTCTACA 
TATGGGTCAA CTCAGCGCCT GGCTGAGTTA TGGCTTGAGA AGAATGACCC AATGTTTGGC 
AGAACCACTC ACACAAATGA ATCTGTGAGG ACAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chsy1 ( XM_031897498.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753358.1 Xenopus tropicalis chondroitin sulfate synthase 1 (chsy1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa53123
Clone ID Related Accession (Same CDS sequence)	XM_031897498.1
Accession Version	XM_031897498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chondroitin sulfate synthase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCA GAAGTCGCCA CTCGTTGCTC AGTGTCCTGC TGGGGCTGGT CCTGGGCTTC 
CTCCTCGCCT CCAGGTTTAT CTTGCCCAGA GCGGCCGAGC TGCAGAGAGC CAGCCGTAGG 
AGAGCGGGCG GTGGGGGGGT ATCAGGGGGG CAGGCTGGGA GCTGTGGCCG ACAATCTGCC 
TTAAGGAACG ATTACAGCGG GGTGCTGGGC TGGCAACAGC GGCTACACAC GGACACCGGG 
GCTCTGAGCC CGGCCAGCGA GGAAGACATC CCCACGGACA AGAACTTCCT TTTTGTTGGG 
GTCATGACAG CTCAGAAGTA CCTGGAAACC AGAGCAGTGG CCGCTTATGG GACTTGGGCA 
AACTCCATTC CTGGTAAAGT TGAATTTTTC TCCAGTGAAG GCTCAGATAC ATCTATACCG 
ATTCCAATTG TGCCACTGCA AGGTGTGGAT GACTCCTATC CACCACAGAA GAAGTCCTTC 
ATGATGCTCA AGTACATGCA TGATCACTAC TTGGATCAAT ATGAGTGGTT TATGCGAGCA 
GATGATGATG TTTACATCAA AGGGGATCGC TTGGAGAACT TTTTGAGGAG CCTCAACAGC 
AGCGAACCAC TGTTCCTTGG CCAGACTGGC CTTGGCACCA CAGAGGAAAT GGGCAAATTG 
GCACTGGAGC CCGGAGAGAA TTTCTGTATG GGTGGCCCTG GTGTGATCAT GAGCAGAGAA 
GTCCTTCGTA GGGTGGTGCC CCACATTGGC GAGTGTCTAA GGCAGATGTT TACCACCCAT 
GAGGATGTGG AGATTGGCAG ATGTGTCAGA AGATTTGCCG GAGTGCAGTG TGTCTGGTCC 
TATGAGATGC AGCAGTTGTT CTATGAGAAT TATGAACAGA ACAAGAAGGG CTACATCCGA 
GATCTTCATA ACAGCAAAAT TCACAGAGCC ATCACTTTAC ATCCCAATAA AAACCCTCCC 
TACCAGTACC GCTTGCACAG CTACATGCTG AGTCGCAAAA TAGCAGAGCT CCGGTATCGT 
ACGATCCAGC TGCACCGGGA GATTGTTCTT ATGAGCAAGT ACAGTAATGC AGAAATCCAT 
AAAGATGATA TGCAACTGGG GATTGCTCCA TCTTTCATGC GGTTCCAACC ACGCCAAAGA 
GAGGAGATTC TGGAGTGGGA ATTTCTCACT GGCAAGTATC TGTATTCAAC TGCTGATGGA 
CAACCTCCTA GACGAGGAAT GGATTCTTCC CAAAAGGAGG CTCTTGATGA CATTGTAATG 
CAAGTTATGG AGATGATAAA TGCCAATGCC AAGGTTCGAG GTCGTATTAT TGACTTTAAA 
GAAATCCAGT ACGGATACCG TAGAGTTAAT CCAATGTATG GTGCAGAATA TGTCTTGGAT 
CTGCTTCTTC TCTACAAAAA GCACAAGGGT AAGAAAATGA CTGTTCCTGT CAGGAGACAT 
GCCTACCTCC AACAGACATT CAGCAAAATT CAGTTCATGG AACATGAGGA GATTGATGCT 
AAAGCGCTGG CGAATAAAAT AAATCAGGAA TCTGGTTCCT TCTACTTTTT GTCAAATTCT 
CTTAAGATGT TTGTTCCCTT TCATCTCACT GCATCGAAGA CTGAACATAA AGAGCCCAAA 
GAAACCAAGG TGAACATCCT GGTTCCCCTC TCTGGCCGAT TTGATATGTT TGCCAGGTTT 
ATGGCCAACT TTGAGAAAAC CTGCCTCATC CCAAATCAAA ATGTGAAGTT AGTCATTCTA 
CTTTTCAATT CTGACTCCAA TCCTGACAAA ACCAGGCAAG TTGAACTCAT GAGAGATTAC 
CGTGTTAAAT ATCCCAAAGC AGATATGCAG ATCTTACCAG TGTCTGGAGA GTTCTCCAGG 
GCTTTGGCTT TAGAAGTTGG ATCCTCGCAG TTTCGAAATG ACTCTCTGCT CTTCTTTTGT 
GATGTGGATT TAGTTTTCTC CACTGAGTTT ATACAACGAT GCAGGGCAAA CACCATTTTA 
GGGCAGCAAA TATACTTTCC AATTATATTT AGCCAATATG ACCCCAAGAT TGTTTATGCT 
GGAAAAGTCC CCAGTGATAA CCATTATGCT TTTACTCAGA AGACTGGATT TTGGAGAAAC 
TATGGCTTTG GCATCACCTG TGTTTATAAG GGAGATCTTT TACGTGCTGG TGGCTTTGAT 
GTTTCCATTC AAGGATGGGG ACTAGAGGAT GTTGACCTTT TCAACAAAGT GGTACAAGTA 
GGTTTAAAGA CCTTTAGGAG TCAGGAGGTT GGAGTCGTTC ACATCCACCA TCCGGTGTTC 
TGTGACCCAA ATCTCGATCC TAAACAGTAC AAAATGTGTT TGGGTTCCAA AGCCTCTACA 
TATGGGTCAA CTCAGCGCCT GGCTGAGTTA TGGCTTGAGA AGAATGACCC AATGTTTGGC 
AGAACCACTC ACACAAATGA ATCTGTGAGG ACAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753358.1
CDS	515..2953
Translation	MAARSRHSLLSVLLGLVLGFLLASRFILPRAAELQRASRRRAGGGGVSGGQAGSCGRQSALRNDYSGVLGWQQRLHTDTGALSPASEEDIPTDKNFLFVGVMTAQKYLETRAVAAYGTWANSIPGKVEFFSSEGSDTSIPIPIVPLQGVDDSYPPQKKSFMMLKYMHDHYLDQYEWFMRADDDVYIKGDRLENFLRSLNSSEPLFLGQTGLGTTEEMGKLALEPGENFCMGGPGVIMSREVLRRVVPHIGECLRQMFTTHEDVEIGRCVRRFAGVQCVWSYEMQQLFYENYEQNKKGYIRDLHNSKIHRAITLHPNKNPPYQYRLHSYMLSRKIAELRYRTIQLHREIVLMSKYSNAEIHKDDMQLGIAPSFMRFQPRQREEILEWEFLTGKYLYSTADGQPPRRGMDSSQKEALDDIVMQVMEMINANAKVRGRIIDFKEIQYGYRRVNPMYGAEYVLDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKIQFMEHEEIDAKALANKINQESGSFYFLSNSLKMFVPFHLTASKTEHKEPKETKVNILVPLSGRFDMFARFMANFEKTCLIPNQNVKLVILLFNSDSNPDKTRQVELMRDYRVKYPKADMQILPVSGEFSRALALEVGSSQFRNDSLLFFCDVDLVFSTEFIQRCRANTILGQQIYFPIIFSQYDPKIVYAGKVPSDNHYAFTQKTGFWRNYGFGITCVYKGDLLRAGGFDVSIQGWGLEDVDLFNKVVQVGLKTFRSQEVGVVHIHHPVFCDPNLDPKQYKMCLGSKASTYGSTQRLAELWLEKNDPMFGRTTHTNESVRTA

Target ORF information:

RefSeq Version	XM_031897498.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chondroitin sulfate synthase 1 (chsy1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897498.1

ORF Insert Sequence:

ATGGCTGCCA GAAGTCGCCA CTCGTTGCTC AGTGTCCTGC TGGGGCTGGT CCTGGGCTTC 
CTCCTCGCCT CCAGGTTTAT CTTGCCCAGA GCGGCCGAGC TGCAGAGAGC CAGCCGTAGG 
AGAGCGGGCG GTGGGGGGGT ATCAGGGGGG CAGGCTGGGA GCTGTGGCCG ACAATCTGCC 
TTAAGGAACG ATTACAGCGG GGTGCTGGGC TGGCAACAGC GGCTACACAC GGACACCGGG 
GCTCTGAGCC CGGCCAGCGA GGAAGACATC CCCACGGACA AGAACTTCCT TTTTGTTGGG 
GTCATGACAG CTCAGAAGTA CCTGGAAACC AGAGCAGTGG CCGCTTATGG GACTTGGGCA 
AACTCCATTC CTGGTAAAGT TGAATTTTTC TCCAGTGAAG GCTCAGATAC ATCTATACCG 
ATTCCAATTG TGCCACTGCA AGGTGTGGAT GACTCCTATC CACCACAGAA GAAGTCCTTC 
ATGATGCTCA AGTACATGCA TGATCACTAC TTGGATCAAT ATGAGTGGTT TATGCGAGCA 
GATGATGATG TTTACATCAA AGGGGATCGC TTGGAGAACT TTTTGAGGAG CCTCAACAGC 
AGCGAACCAC TGTTCCTTGG CCAGACTGGC CTTGGCACCA CAGAGGAAAT GGGCAAATTG 
GCACTGGAGC CCGGAGAGAA TTTCTGTATG GGTGGCCCTG GTGTGATCAT GAGCAGAGAA 
GTCCTTCGTA GGGTGGTGCC CCACATTGGC GAGTGTCTAA GGCAGATGTT TACCACCCAT 
GAGGATGTGG AGATTGGCAG ATGTGTCAGA AGATTTGCCG GAGTGCAGTG TGTCTGGTCC 
TATGAGATGC AGCAGTTGTT CTATGAGAAT TATGAACAGA ACAAGAAGGG CTACATCCGA 
GATCTTCATA ACAGCAAAAT TCACAGAGCC ATCACTTTAC ATCCCAATAA AAACCCTCCC 
TACCAGTACC GCTTGCACAG CTACATGCTG AGTCGCAAAA TAGCAGAGCT CCGGTATCGT 
ACGATCCAGC TGCACCGGGA GATTGTTCTT ATGAGCAAGT ACAGTAATGC AGAAATCCAT 
AAAGATGATA TGCAACTGGG GATTGCTCCA TCTTTCATGC GGTTCCAACC ACGCCAAAGA 
GAGGAGATTC TGGAGTGGGA ATTTCTCACT GGCAAGTATC TGTATTCAAC TGCTGATGGA 
CAACCTCCTA GACGAGGAAT GGATTCTTCC CAAAAGGAGG CTCTTGATGA CATTGTAATG 
CAAGTTATGG AGATGATAAA TGCCAATGCC AAGGTTCGAG GTCGTATTAT TGACTTTAAA 
GAAATCCAGT ACGGATACCG TAGAGTTAAT CCAATGTATG GTGCAGAATA TGTCTTGGAT 
CTGCTTCTTC TCTACAAAAA GCACAAGGGT AAGAAAATGA CTGTTCCTGT CAGGAGACAT 
GCCTACCTCC AACAGACATT CAGCAAAATT CAGTTCATGG AACATGAGGA GATTGATGCT 
AAAGCGCTGG CGAATAAAAT AAATCAGGAA TCTGGTTCCT TCTACTTTTT GTCAAATTCT 
CTTAAGATGT TTGTTCCCTT TCATCTCACT GCATCGAAGA CTGAACATAA AGAGCCCAAA 
GAAACCAAGG TGAACATCCT GGTTCCCCTC TCTGGCCGAT TTGATATGTT TGCCAGGTTT 
ATGGCCAACT TTGAGAAAAC CTGCCTCATC CCAAATCAAA ATGTGAAGTT AGTCATTCTA 
CTTTTCAATT CTGACTCCAA TCCTGACAAA ACCAGGCAAG TTGAACTCAT GAGAGATTAC 
CGTGTTAAAT ATCCCAAAGC AGATATGCAG ATCTTACCAG TGTCTGGAGA GTTCTCCAGG 
GCTTTGGCTT TAGAAGTTGG ATCCTCGCAG TTTCGAAATG ACTCTCTGCT CTTCTTTTGT 
GATGTGGATT TAGTTTTCTC CACTGAGTTT ATACAACGAT GCAGGGCAAA CACCATTTTA 
GGGCAGCAAA TATACTTTCC AATTATATTT AGCCAATATG ACCCCAAGAT TGTTTATGCT 
GGAAAAGTCC CCAGTGATAA CCATTATGCT TTTACTCAGA AGACTGGATT TTGGAGAAAC 
TATGGCTTTG GCATCACCTG TGTTTATAAG GGAGATCTTT TACGTGCTGG TGGCTTTGAT 
GTTTCCATTC AAGGATGGGG ACTAGAGGAT GTTGACCTTT TCAACAAAGT GGTACAAGTA 
GGTTTAAAGA CCTTTAGGAG TCAGGAGGTT GGAGTCGTTC ACATCCACCA TCCGGTGTTC 
TGTGACCCAA ATCTCGATCC TAAACAGTAC AAAATGTGTT TGGGTTCCAA AGCCTCTACA 
TATGGGTCAA CTCAGCGCCT GGCTGAGTTA TGGCTTGAGA AGAATGACCC AATGTTTGGC 
AGAACCACTC ACACAAATGA ATCTGTGAGG ACAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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chsy1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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