znf516 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
NM_001097250.1	NP_001090719.1	zinc finger protein 516
XM_018089400.1	XP_017944889.1	zinc finger protein 516 isoform X1
XM_018089401.1	XP_017944890.1	zinc finger protein 516 isoform X2
XM_012964127.2	XP_012819581.1	zinc finger protein 516 isoform X3
XM_031903141.1	XP_031759001.1	zinc finger protein 516 isoform X2
XM_018089396.1	XP_017944885.1	zinc finger protein 516 isoform X1
XM_018089398.1	XP_017944887.1	zinc finger protein 516 isoform X1
XM_018089397.1	XP_017944886.1	zinc finger protein 516 isoform X1
XM_018089399.1	XP_017944888.1	zinc finger protein 516 isoform X1
XM_012964127.3	XP_012819581.2	zinc finger protein 516 isoform X1
XM_031903140.1	XP_031759000.1	zinc finger protein 516 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa40660	XM_018089396.1 Latest version!	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa40660	XM_018089400.1 Latest version!	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa40660	XM_018089397.1 Latest version!	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa40660	XM_018089398.1 Latest version!	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa40660	XM_018089399.1 Latest version!	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa40661	XM_018089401.1 Latest version!	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa59449	XM_031903140.1 Latest version!	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa59449	XM_012964127.3 Latest version!	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa59450	XM_031903141.1 Latest version!	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa23389	NM_001097250.1 Latest version!	Xenopus tropicalis zinc finger protein 516 (znf516), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa23389	XM_012964127.2 Latest version!	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

znf516 ( XM_018089400.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944889.1 Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa40660
Clone ID Related Accession (Same CDS sequence)	XM_018089400.1 , XM_018089396.1 , XM_018089398.1 , XM_018089397.1 , XM_018089399.1
Accession Version	XM_018089400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 516 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683375.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG GGGAGAGACC ATTCCAGTGC AGATATTGTC CATACAGTGC CTCTCAAAAA 
GGGAACCTAA AAACACACGT ACAGTGTGTA CATCGGGTAC CATTTGACAA TAGTGAGTAC 
CCTGACAAAC AGCTCCTTCA ACCTCAAAGC GATGATAACA GTTACATGGA CGATCAGCTG 
GAAAATTTGC CTTCAAATCA CCAAGTCCCT GGAGCTGTGG CGTTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944889.1
CDS	953..4603
Translation	MERRKQFDMSLRESSAKSGKNDTEEDKGSGFSCVICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRAAQKGNLKIHLRTHRSGTINQVTDMEMGDLHVGEFGASEGMGGCASPTKSTSACNKILNGTLQTEEAEEVDDDQLGNFQCPFCKNKYDRKKELDQHLLQVHKPYKCRFCSYMTLREETLLNHIEKDHITTAVPLNRDGLAENGKTEINSGEFPCEVCGQAFGQAWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGNKNKPKSDSEPVATINDVVQEETIMTGLSLYEVCMKCGNLFTNLESLNAHNLVHCKTEEISLEDPIEQNRENDTPNSMGAKQFLLDYLHLRPLKDANKPSDIQQGKRVPELDPINSYQAWQLATKGKVAEPSEYVKYLGWDETMADADVTYDKDKGEYILVNQEKRKREPDSQSTSNPKKRNSSSVSRSEKSSNTQSGENVDGSNELEFRPPSRQSRRSSQNKSTECFECGKVFRTYHQMVLHSRVHRKDRRSLSESGSVSHNDRYGSNSDIDSASRPSSPDSASAPEDSLASGMGEDAADDDSFEEGPTPSPVRRHLIYEEMYKSTKGHLPAYLSVTHFVETCRDSQGIKPYHLTFSEGVPMEMSVNHQQNFTSDDTSKCEGSKCIPEVQTPPAFISNKHADFPFIPDLKIPAFKYNQDPSASGKHTPNSSSSFSVGHSSEVIVDAKKVFSAEKGHPNGMALDMLKEHSGSDKSSGGKHDLPLDLSEKSTRHELCSKSAISALQAALVVHQCPYCSHNTYYPEVLWMHKRIWHKVSCNSMAPQWIQQNGFKNLKNNLLFLTRSGRTGPPPALGGKECQPLPIARFSRTQMPIRPPTSKGNTANLSSVSKAACGSQVRDSPHGFSGAKTTNFEVQRHQKVGDQYSAAVQQPLLKSKYETSPKLMQAASFSRNLSPAQASVSRQILQPSSSKQVEKYGMPQGGPSTFASPSKLSVSDTIKTKFPLQQPQYPFHKVEPQVKQESPPVPHRESQGKIVNELGTVSNCSTGMKTSPLFHSQPSTAGDPQSFQPVKQEPVSEGHEKRLDILNIFKTYIPKDLATLYQSWGANANNLDNAGMLRTQARQGDYVCNECGKCFSQPSHLRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQCVHRVPFDNSEYPDKQLLQPQSDDNSYMDDQLENLPSNHQVPGAVALK

Target ORF information:

RefSeq Version	XM_018089400.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089400.1

ORF Insert Sequence:

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG GGGAGAGACC ATTCCAGTGC AGATATTGTC CATACAGTGC CTCTCAAAAA 
GGGAACCTAA AAACACACGT ACAGTGTGTA CATCGGGTAC CATTTGACAA TAGTGAGTAC 
CCTGACAAAC AGCTCCTTCA ACCTCAAAGC GATGATAACA GTTACATGGA CGATCAGCTG 
GAAAATTTGC CTTCAAATCA CCAAGTCCCT GGAGCTGTGG CGTTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_018089396.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944885.1 Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa40660
Clone ID Related Accession (Same CDS sequence)	XM_018089400.1 , XM_018089396.1 , XM_018089398.1 , XM_018089397.1 , XM_018089399.1
Accession Version	XM_018089396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 516 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683375.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG GGGAGAGACC ATTCCAGTGC AGATATTGTC CATACAGTGC CTCTCAAAAA 
GGGAACCTAA AAACACACGT ACAGTGTGTA CATCGGGTAC CATTTGACAA TAGTGAGTAC 
CCTGACAAAC AGCTCCTTCA ACCTCAAAGC GATGATAACA GTTACATGGA CGATCAGCTG 
GAAAATTTGC CTTCAAATCA CCAAGTCCCT GGAGCTGTGG CGTTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944885.1
CDS	1054..4704
Translation	MERRKQFDMSLRESSAKSGKNDTEEDKGSGFSCVICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRAAQKGNLKIHLRTHRSGTINQVTDMEMGDLHVGEFGASEGMGGCASPTKSTSACNKILNGTLQTEEAEEVDDDQLGNFQCPFCKNKYDRKKELDQHLLQVHKPYKCRFCSYMTLREETLLNHIEKDHITTAVPLNRDGLAENGKTEINSGEFPCEVCGQAFGQAWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGNKNKPKSDSEPVATINDVVQEETIMTGLSLYEVCMKCGNLFTNLESLNAHNLVHCKTEEISLEDPIEQNRENDTPNSMGAKQFLLDYLHLRPLKDANKPSDIQQGKRVPELDPINSYQAWQLATKGKVAEPSEYVKYLGWDETMADADVTYDKDKGEYILVNQEKRKREPDSQSTSNPKKRNSSSVSRSEKSSNTQSGENVDGSNELEFRPPSRQSRRSSQNKSTECFECGKVFRTYHQMVLHSRVHRKDRRSLSESGSVSHNDRYGSNSDIDSASRPSSPDSASAPEDSLASGMGEDAADDDSFEEGPTPSPVRRHLIYEEMYKSTKGHLPAYLSVTHFVETCRDSQGIKPYHLTFSEGVPMEMSVNHQQNFTSDDTSKCEGSKCIPEVQTPPAFISNKHADFPFIPDLKIPAFKYNQDPSASGKHTPNSSSSFSVGHSSEVIVDAKKVFSAEKGHPNGMALDMLKEHSGSDKSSGGKHDLPLDLSEKSTRHELCSKSAISALQAALVVHQCPYCSHNTYYPEVLWMHKRIWHKVSCNSMAPQWIQQNGFKNLKNNLLFLTRSGRTGPPPALGGKECQPLPIARFSRTQMPIRPPTSKGNTANLSSVSKAACGSQVRDSPHGFSGAKTTNFEVQRHQKVGDQYSAAVQQPLLKSKYETSPKLMQAASFSRNLSPAQASVSRQILQPSSSKQVEKYGMPQGGPSTFASPSKLSVSDTIKTKFPLQQPQYPFHKVEPQVKQESPPVPHRESQGKIVNELGTVSNCSTGMKTSPLFHSQPSTAGDPQSFQPVKQEPVSEGHEKRLDILNIFKTYIPKDLATLYQSWGANANNLDNAGMLRTQARQGDYVCNECGKCFSQPSHLRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQCVHRVPFDNSEYPDKQLLQPQSDDNSYMDDQLENLPSNHQVPGAVALK

Target ORF information:

RefSeq Version	XM_018089396.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089396.1

ORF Insert Sequence:

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG GGGAGAGACC ATTCCAGTGC AGATATTGTC CATACAGTGC CTCTCAAAAA 
GGGAACCTAA AAACACACGT ACAGTGTGTA CATCGGGTAC CATTTGACAA TAGTGAGTAC 
CCTGACAAAC AGCTCCTTCA ACCTCAAAGC GATGATAACA GTTACATGGA CGATCAGCTG 
GAAAATTTGC CTTCAAATCA CCAAGTCCCT GGAGCTGTGG CGTTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_018089398.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944887.1 Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X3, mRNA.

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CloneID	OXa40660
Clone ID Related Accession (Same CDS sequence)	XM_018089400.1 , XM_018089396.1 , XM_018089398.1 , XM_018089397.1 , XM_018089399.1
Accession Version	XM_018089398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 516 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683375.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG GGGAGAGACC ATTCCAGTGC AGATATTGTC CATACAGTGC CTCTCAAAAA 
GGGAACCTAA AAACACACGT ACAGTGTGTA CATCGGGTAC CATTTGACAA TAGTGAGTAC 
CCTGACAAAC AGCTCCTTCA ACCTCAAAGC GATGATAACA GTTACATGGA CGATCAGCTG 
GAAAATTTGC CTTCAAATCA CCAAGTCCCT GGAGCTGTGG CGTTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944887.1
CDS	398..4048
Translation	MERRKQFDMSLRESSAKSGKNDTEEDKGSGFSCVICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRAAQKGNLKIHLRTHRSGTINQVTDMEMGDLHVGEFGASEGMGGCASPTKSTSACNKILNGTLQTEEAEEVDDDQLGNFQCPFCKNKYDRKKELDQHLLQVHKPYKCRFCSYMTLREETLLNHIEKDHITTAVPLNRDGLAENGKTEINSGEFPCEVCGQAFGQAWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGNKNKPKSDSEPVATINDVVQEETIMTGLSLYEVCMKCGNLFTNLESLNAHNLVHCKTEEISLEDPIEQNRENDTPNSMGAKQFLLDYLHLRPLKDANKPSDIQQGKRVPELDPINSYQAWQLATKGKVAEPSEYVKYLGWDETMADADVTYDKDKGEYILVNQEKRKREPDSQSTSNPKKRNSSSVSRSEKSSNTQSGENVDGSNELEFRPPSRQSRRSSQNKSTECFECGKVFRTYHQMVLHSRVHRKDRRSLSESGSVSHNDRYGSNSDIDSASRPSSPDSASAPEDSLASGMGEDAADDDSFEEGPTPSPVRRHLIYEEMYKSTKGHLPAYLSVTHFVETCRDSQGIKPYHLTFSEGVPMEMSVNHQQNFTSDDTSKCEGSKCIPEVQTPPAFISNKHADFPFIPDLKIPAFKYNQDPSASGKHTPNSSSSFSVGHSSEVIVDAKKVFSAEKGHPNGMALDMLKEHSGSDKSSGGKHDLPLDLSEKSTRHELCSKSAISALQAALVVHQCPYCSHNTYYPEVLWMHKRIWHKVSCNSMAPQWIQQNGFKNLKNNLLFLTRSGRTGPPPALGGKECQPLPIARFSRTQMPIRPPTSKGNTANLSSVSKAACGSQVRDSPHGFSGAKTTNFEVQRHQKVGDQYSAAVQQPLLKSKYETSPKLMQAASFSRNLSPAQASVSRQILQPSSSKQVEKYGMPQGGPSTFASPSKLSVSDTIKTKFPLQQPQYPFHKVEPQVKQESPPVPHRESQGKIVNELGTVSNCSTGMKTSPLFHSQPSTAGDPQSFQPVKQEPVSEGHEKRLDILNIFKTYIPKDLATLYQSWGANANNLDNAGMLRTQARQGDYVCNECGKCFSQPSHLRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQCVHRVPFDNSEYPDKQLLQPQSDDNSYMDDQLENLPSNHQVPGAVALK

Target ORF information:

RefSeq Version	XM_018089398.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089398.1

ORF Insert Sequence:

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG GGGAGAGACC ATTCCAGTGC AGATATTGTC CATACAGTGC CTCTCAAAAA 
GGGAACCTAA AAACACACGT ACAGTGTGTA CATCGGGTAC CATTTGACAA TAGTGAGTAC 
CCTGACAAAC AGCTCCTTCA ACCTCAAAGC GATGATAACA GTTACATGGA CGATCAGCTG 
GAAAATTTGC CTTCAAATCA CCAAGTCCCT GGAGCTGTGG CGTTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_018089397.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944886.1 Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa40660
Clone ID Related Accession (Same CDS sequence)	XM_018089400.1 , XM_018089396.1 , XM_018089398.1 , XM_018089397.1 , XM_018089399.1
Accession Version	XM_018089397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 516 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683375.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG GGGAGAGACC ATTCCAGTGC AGATATTGTC CATACAGTGC CTCTCAAAAA 
GGGAACCTAA AAACACACGT ACAGTGTGTA CATCGGGTAC CATTTGACAA TAGTGAGTAC 
CCTGACAAAC AGCTCCTTCA ACCTCAAAGC GATGATAACA GTTACATGGA CGATCAGCTG 
GAAAATTTGC CTTCAAATCA CCAAGTCCCT GGAGCTGTGG CGTTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944886.1
CDS	1138..4788
Translation	MERRKQFDMSLRESSAKSGKNDTEEDKGSGFSCVICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRAAQKGNLKIHLRTHRSGTINQVTDMEMGDLHVGEFGASEGMGGCASPTKSTSACNKILNGTLQTEEAEEVDDDQLGNFQCPFCKNKYDRKKELDQHLLQVHKPYKCRFCSYMTLREETLLNHIEKDHITTAVPLNRDGLAENGKTEINSGEFPCEVCGQAFGQAWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGNKNKPKSDSEPVATINDVVQEETIMTGLSLYEVCMKCGNLFTNLESLNAHNLVHCKTEEISLEDPIEQNRENDTPNSMGAKQFLLDYLHLRPLKDANKPSDIQQGKRVPELDPINSYQAWQLATKGKVAEPSEYVKYLGWDETMADADVTYDKDKGEYILVNQEKRKREPDSQSTSNPKKRNSSSVSRSEKSSNTQSGENVDGSNELEFRPPSRQSRRSSQNKSTECFECGKVFRTYHQMVLHSRVHRKDRRSLSESGSVSHNDRYGSNSDIDSASRPSSPDSASAPEDSLASGMGEDAADDDSFEEGPTPSPVRRHLIYEEMYKSTKGHLPAYLSVTHFVETCRDSQGIKPYHLTFSEGVPMEMSVNHQQNFTSDDTSKCEGSKCIPEVQTPPAFISNKHADFPFIPDLKIPAFKYNQDPSASGKHTPNSSSSFSVGHSSEVIVDAKKVFSAEKGHPNGMALDMLKEHSGSDKSSGGKHDLPLDLSEKSTRHELCSKSAISALQAALVVHQCPYCSHNTYYPEVLWMHKRIWHKVSCNSMAPQWIQQNGFKNLKNNLLFLTRSGRTGPPPALGGKECQPLPIARFSRTQMPIRPPTSKGNTANLSSVSKAACGSQVRDSPHGFSGAKTTNFEVQRHQKVGDQYSAAVQQPLLKSKYETSPKLMQAASFSRNLSPAQASVSRQILQPSSSKQVEKYGMPQGGPSTFASPSKLSVSDTIKTKFPLQQPQYPFHKVEPQVKQESPPVPHRESQGKIVNELGTVSNCSTGMKTSPLFHSQPSTAGDPQSFQPVKQEPVSEGHEKRLDILNIFKTYIPKDLATLYQSWGANANNLDNAGMLRTQARQGDYVCNECGKCFSQPSHLRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQCVHRVPFDNSEYPDKQLLQPQSDDNSYMDDQLENLPSNHQVPGAVALK

Target ORF information:

RefSeq Version	XM_018089397.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089397.1

ORF Insert Sequence:

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG GGGAGAGACC ATTCCAGTGC AGATATTGTC CATACAGTGC CTCTCAAAAA 
GGGAACCTAA AAACACACGT ACAGTGTGTA CATCGGGTAC CATTTGACAA TAGTGAGTAC 
CCTGACAAAC AGCTCCTTCA ACCTCAAAGC GATGATAACA GTTACATGGA CGATCAGCTG 
GAAAATTTGC CTTCAAATCA CCAAGTCCCT GGAGCTGTGG CGTTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_018089399.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944888.1 Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa40660
Clone ID Related Accession (Same CDS sequence)	XM_018089400.1 , XM_018089396.1 , XM_018089398.1 , XM_018089397.1 , XM_018089399.1
Accession Version	XM_018089399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 516 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683375.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG GGGAGAGACC ATTCCAGTGC AGATATTGTC CATACAGTGC CTCTCAAAAA 
GGGAACCTAA AAACACACGT ACAGTGTGTA CATCGGGTAC CATTTGACAA TAGTGAGTAC 
CCTGACAAAC AGCTCCTTCA ACCTCAAAGC GATGATAACA GTTACATGGA CGATCAGCTG 
GAAAATTTGC CTTCAAATCA CCAAGTCCCT GGAGCTGTGG CGTTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944888.1
CDS	284..3934
Translation	MERRKQFDMSLRESSAKSGKNDTEEDKGSGFSCVICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRAAQKGNLKIHLRTHRSGTINQVTDMEMGDLHVGEFGASEGMGGCASPTKSTSACNKILNGTLQTEEAEEVDDDQLGNFQCPFCKNKYDRKKELDQHLLQVHKPYKCRFCSYMTLREETLLNHIEKDHITTAVPLNRDGLAENGKTEINSGEFPCEVCGQAFGQAWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGNKNKPKSDSEPVATINDVVQEETIMTGLSLYEVCMKCGNLFTNLESLNAHNLVHCKTEEISLEDPIEQNRENDTPNSMGAKQFLLDYLHLRPLKDANKPSDIQQGKRVPELDPINSYQAWQLATKGKVAEPSEYVKYLGWDETMADADVTYDKDKGEYILVNQEKRKREPDSQSTSNPKKRNSSSVSRSEKSSNTQSGENVDGSNELEFRPPSRQSRRSSQNKSTECFECGKVFRTYHQMVLHSRVHRKDRRSLSESGSVSHNDRYGSNSDIDSASRPSSPDSASAPEDSLASGMGEDAADDDSFEEGPTPSPVRRHLIYEEMYKSTKGHLPAYLSVTHFVETCRDSQGIKPYHLTFSEGVPMEMSVNHQQNFTSDDTSKCEGSKCIPEVQTPPAFISNKHADFPFIPDLKIPAFKYNQDPSASGKHTPNSSSSFSVGHSSEVIVDAKKVFSAEKGHPNGMALDMLKEHSGSDKSSGGKHDLPLDLSEKSTRHELCSKSAISALQAALVVHQCPYCSHNTYYPEVLWMHKRIWHKVSCNSMAPQWIQQNGFKNLKNNLLFLTRSGRTGPPPALGGKECQPLPIARFSRTQMPIRPPTSKGNTANLSSVSKAACGSQVRDSPHGFSGAKTTNFEVQRHQKVGDQYSAAVQQPLLKSKYETSPKLMQAASFSRNLSPAQASVSRQILQPSSSKQVEKYGMPQGGPSTFASPSKLSVSDTIKTKFPLQQPQYPFHKVEPQVKQESPPVPHRESQGKIVNELGTVSNCSTGMKTSPLFHSQPSTAGDPQSFQPVKQEPVSEGHEKRLDILNIFKTYIPKDLATLYQSWGANANNLDNAGMLRTQARQGDYVCNECGKCFSQPSHLRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQCVHRVPFDNSEYPDKQLLQPQSDDNSYMDDQLENLPSNHQVPGAVALK

Target ORF information:

RefSeq Version	XM_018089399.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089399.1

ORF Insert Sequence:

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG GGGAGAGACC ATTCCAGTGC AGATATTGTC CATACAGTGC CTCTCAAAAA 
GGGAACCTAA AAACACACGT ACAGTGTGTA CATCGGGTAC CATTTGACAA TAGTGAGTAC 
CCTGACAAAC AGCTCCTTCA ACCTCAAAGC GATGATAACA GTTACATGGA CGATCAGCTG 
GAAAATTTGC CTTCAAATCA CCAAGTCCCT GGAGCTGTGG CGTTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_018089401.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944890.1 Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X6, mRNA.

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CloneID	OXa40661
Clone ID Related Accession (Same CDS sequence)	XM_018089401.1
Accession Version	XM_018089401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 516 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683375.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG TGGTATTTGA GTCTAATGGA CTCCGAGGTA CTGAAGTTCA CACCACCTCC 
GCAGATGCCC CAAAACAAGG GAGAGACCAT TCCAGTGCAG ATATTGTCCA TACAGTGCCT 
CTCAAAAAGG GAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944890.1
CDS	1054..4611
Translation	MERRKQFDMSLRESSAKSGKNDTEEDKGSGFSCVICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRAAQKGNLKIHLRTHRSGTINQVTDMEMGDLHVGEFGASEGMGGCASPTKSTSACNKILNGTLQTEEAEEVDDDQLGNFQCPFCKNKYDRKKELDQHLLQVHKPYKCRFCSYMTLREETLLNHIEKDHITTAVPLNRDGLAENGKTEINSGEFPCEVCGQAFGQAWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGNKNKPKSDSEPVATINDVVQEETIMTGLSLYEVCMKCGNLFTNLESLNAHNLVHCKTEEISLEDPIEQNRENDTPNSMGAKQFLLDYLHLRPLKDANKPSDIQQGKRVPELDPINSYQAWQLATKGKVAEPSEYVKYLGWDETMADADVTYDKDKGEYILVNQEKRKREPDSQSTSNPKKRNSSSVSRSEKSSNTQSGENVDGSNELEFRPPSRQSRRSSQNKSTECFECGKVFRTYHQMVLHSRVHRKDRRSLSESGSVSHNDRYGSNSDIDSASRPSSPDSASAPEDSLASGMGEDAADDDSFEEGPTPSPVRRHLIYEEMYKSTKGHLPAYLSVTHFVETCRDSQGIKPYHLTFSEGVPMEMSVNHQQNFTSDDTSKCEGSKCIPEVQTPPAFISNKHADFPFIPDLKIPAFKYNQDPSASGKHTPNSSSSFSVGHSSEVIVDAKKVFSAEKGHPNGMALDMLKEHSGSDKSSGGKHDLPLDLSEKSTRHELCSKSAISALQAALVVHQCPYCSHNTYYPEVLWMHKRIWHKVSCNSMAPQWIQQNGFKNLKNNLLFLTRSGRTGPPPALGGKECQPLPIARFSRTQMPIRPPTSKGNTANLSSVSKAACGSQVRDSPHGFSGAKTTNFEVQRHQKVGDQYSAAVQQPLLKSKYETSPKLMQAASFSRNLSPAQASVSRQILQPSSSKQVEKYGMPQGGPSTFASPSKLSVSDTIKTKFPLQQPQYPFHKVEPQVKQESPPVPHRESQGKIVNELGTVSNCSTGMKTSPLFHSQPSTAGDPQSFQPVKQEPVSEGHEKRLDILNIFKTYIPKDLATLYQSWGANANNLDNAGMLRTQARQGDYVCNECGKCFSQPSHLRTHMRSHTVVFESNGLRGTEVHTTSADAPKQGRDHSSADIVHTVPLKKGT

Target ORF information:

RefSeq Version	XM_018089401.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089401.1

ORF Insert Sequence:

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGTAAGAAGA CACTTAATTT ATGAAGAGAT GTACAAAAGC 
ACTAAGGGGC ACCTTCCTGC CTACCTTTCA GTGACACATT TTGTAGAGAC CTGCAGGGAT 
TCACAAGGCA TTAAACCATA CCATCTTACA TTTTCTGAGG GTGTACCCAT GGAAATGTCA 
GTAAATCATC AACAGAACTT CACTTCTGAT GATACCAGCA AATGTGAAGG AAGTAAATGT 
ATTCCAGAGG TGCAGACTCC TCCAGCCTTC ATATCTAATA AGCATGCTGA TTTTCCTTTT 
ATCCCTGATT TGAAAATACC AGCATTCAAG TACAATCAAG ATCCCAGTGC TTCTGGAAAG 
CATACCCCGA ACAGTAGTTC ATCTTTTTCT GTGGGACATA GTTCAGAAGT AATTGTGGAT 
GCAAAAAAAG TTTTTTCAGC AGAAAAGGGT CACCCAAATG GAATGGCACT TGATATGCTG 
AAGGAACACA GTGGGTCAGA TAAAAGCAGT GGTGGAAAAC ACGACCTTCC TTTGGATCTT 
AGTGAAAAAT CTACTAGGCA TGAACTTTGT AGTAAAAGTG CTATTTCTGC ATTGCAGGCA 
GCGTTGGTGG TTCACCAGTG TCCATATTGC AGCCACAATA CTTATTATCC TGAGGTGCTG 
TGGATGCACA AAAGAATTTG GCATAAAGTT AGTTGTAATT CAATGGCTCC TCAATGGATT 
CAGCAAAATG GTTTCAAAAA TTTGAAAAAT AATTTGCTTT TCCTAACTAG GAGTGGGCGT 
ACAGGTCCGC CTCCAGCACT TGGTGGTAAA GAATGTCAGC CTTTACCCAT AGCTAGGTTC 
TCACGCACTC AAATGCCAAT TCGGCCTCCA ACATCCAAAG GAAATACTGC AAATCTAAGC 
AGTGTTTCCA AGGCAGCATG TGGATCTCAG GTTCGTGACA GCCCTCATGG ATTCAGTGGG 
GCGAAAACCA CAAACTTTGA AGTCCAGAGG CACCAAAAGG TAGGGGACCA ATATAGTGCT 
GCTGTACAGC AACCATTGTT AAAATCTAAA TATGAAACTA GTCCAAAATT GATGCAAGCT 
GCAAGTTTCA GTAGAAACCT TTCACCTGCT CAAGCATCAG TATCAAGACA AATACTTCAG 
CCCTCTAGTA GCAAACAAGT GGAGAAATAC GGGATGCCTC AAGGTGGACC TAGTACATTT 
GCATCTCCGA GTAAACTATC TGTATCTGAC ACAATTAAAA CCAAATTTCC TCTTCAGCAG 
CCACAGTACC CATTTCACAA AGTGGAGCCA CAAGTTAAAC AGGAGAGTCC TCCAGTGCCT 
CACCGGGAAT CTCAGGGCAA GATTGTCAAT GAACTAGGGA CAGTGTCCAA CTGTAGCACA 
GGCATGAAGA CAAGCCCTTT GTTTCATTCT CAACCAAGTA CAGCAGGTGA CCCCCAATCT 
TTTCAACCGG TCAAGCAAGA ACCAGTATCT GAAGGCCATG AAAAACGACT GGACATTTTA 
AATATCTTTA AGACCTACAT TCCAAAGGAT CTTGCTACTC TCTACCAAAG CTGGGGTGCC 
AACGCTAATA ATTTGGATAA TGCAGGAATG CTTAGGACAC AAGCACGGCA AGGAGACTAT 
GTTTGCAATG AGTGCGGAAA ATGTTTCAGC CAACCAAGCC ACTTAAGAAC CCATATGAGG 
TCCCATACAG TGGTATTTGA GTCTAATGGA CTCCGAGGTA CTGAAGTTCA CACCACCTCC 
GCAGATGCCC CAAAACAAGG GAGAGACCAT TCCAGTGCAG ATATTGTCCA TACAGTGCCT 
CTCAAAAAGG GAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_012964127.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819581.2 Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa59449
Clone ID Related Accession (Same CDS sequence)	XM_012964127.3 , XM_031903140.1
Accession Version	XM_012964127.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 516 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964127.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGGCATTAAA CCATACCATC TTACATTTTC TGAGGGTGTA 
CCCATGGAAA TGTCAGTAAA TCATCAACAG AACTTCACTT CTGATGATAC CAGCAAATGT 
GAAGGAAGTA AATGTATTCC AGAGGTGCAG ACTCCTCCAG CCTTCATATC TAATAAGCAT 
GCTGATTTTC CTTTTATCCC TGATTTGAAA ATACCAGCAT TCAAGTACAA TCAAGATCCC 
AGTGCTTCTG GAAAGCATAC CCCGAACAGT AGTTCATCTT TTTCTGTGGG ACATAGTTCA 
GAAGTAATTG TGGATGCAAA AAAAGTTTTT TCAGCAGAAA AGGGTCACCC AAATGGAATG 
GCACTTGATA TGCTGAAGGA ACACAGTGGG TCAGATAAAA GCAGTGGTGG AAAACACGAC 
CTTCCTTTGG ATCTTAGTGA AAAATCTACT AGGCATGAAC TTTGTAGTAA AAGTGCTATT 
TCTGCATTGC AGGCAGCGTT GGTGGTTCAC CAGTGTCCAT ATTGCAGCCA CAATACTTAT 
TATCCTGAGG TGCTGTGGAT GCACAAAAGA ATTTGGCATA AAGTTAGTTG TAATTCAATG 
GCTCCTCAAT GGATTCAGCA AAATGGTTTC AAAAATTTGA AAAATAATTT GCTTTTCCTA 
ACTAGGAGTG GGCGTACAGG TCCGCCTCCA GCACTTGGTG GTAAAGAATG TCAGCCTTTA 
CCCATAGCTA GGTTCTCACG CACTCAAATG CCAATTCGGC CTCCAACATC CAAAGGAAAT 
ACTGCAAATC TAAGCAGTGT TTCCAAGGCA GCATGTGGAT CTCAGGTTCG TGACAGCCCT 
CATGGATTCA GTGGGGCGAA AACCGCAAAC TTTGAAGTCC AGAGGCACCA AAAGGTAGGG 
GACCAATATA GTGCTGCTGT ACAGCAACCA TTGTTAAAAT CTAAATATGA AACTAGTCCA 
AAATTGATGC AAGCTGCAAG TTTCAGTAGA AACCTTTCAC CTGCTCAAGC ATCAGTATCA 
AGACAAATAC TTCAGCCCTC TAGTAGCAAA CAAGTGGAGA AATACGGGAT GCCTCAAGGT 
GGACCTAGTA CATTTGCATC TCCGAGTAAA CTATCTGTAT CTGACACAAT TAAAACCAAA 
TTTCCTCTTC AGCAGCCACA GTACCCATTT CACAAAGTGG AGCCACAAGT TAAACAGGAG 
AGTCCTCCAG TGCCTCACCG GGAATCTCAG GGCAAGATTG TCAATGAACT AGGGACAGTG 
TCCAACTGTA GCACAGGCAT GAAGACAAGC CCTTTGTTTC ATTCTCAACC AAGTACAGCA 
GGTGACCCCC AATCTTTTCA ACCGGTCAAG CAAGAACCAG TATCTGAAGG CCATGAAAAA 
CGACTGGACA TTTTAAATAT CTTTAAGACC TACATTCCAA AGGATCTTGC TACTCTCTAC 
CAAAGCTGGG GTGCCAACGC TAATAATTTG GATAATGCAG GAATGCTTAG GACACAAGCA 
CGGCAAGGAG ACTATGTTTG CAATGAGTGC GGAAAATGTT TCAGCCAACC AAGCCACTTA 
AGAACCCATA TGAGGTCCCA TACAGGGGAG AGACCATTCC AGTGCAGATA TTGTCCATAC 
AGTGCCTCTC AAAAAGGGAA CCTAAAAACA CACGTACAGT GTGTACATCG GGTACCATTT 
GACAATAGTG AGTACCCTGA CAAACAGCTC CTTCAACCTC AAAGCGATGA TAACAGTTAC 
ATGGACGATC AGCTGGAAAA TTTGCCTTCA AATCACCAAG TCCCTGGAGC TGTGGCGTTA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819581.2
CDS	714..4259
Translation	MERRKQFDMSLRESSAKSGKNDTEEDKGSGFSCVICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRAAQKGNLKIHLRTHRSGTINQVTDMEMGDLHVGEFGASEGMGGCASPTKSTSACNKILNGTLQTEEAEEVDDDQLGNFQCPFCKNKYDRKKELDQHLLQVHKPYKCRFCSYMTLREETLLNHIEKDHITTAVPLNRDGLAENGKTEINSGEFPCEVCGQAFGQAWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGNKNKPKSDSEPVATINDVVQEETIMTGLSLYEVCMKCGNLFTNLESLNAHNLVHCKTEEISLEDPIEQNRENDTPNSMGAKQFLLDYLHLRPLKDANKPSDIQQGKRVPELDPINSYQAWQLATKGKVAEPSEYVKYLGWDETMADADVTYDKDKGEYILVNQEKRKREPDSQSTSNPKKRNSSSVSRSEKSSNTQSGENVDGSNELEFRPPSRQSRRSSQNKSTECFECGKVFRTYHQMVLHSRVHRKDRRSLSESGSVSHNDRYGSNSDIDSASRPSSPDSASAPEDSLASGMGEDAADDDSFEEGPTPSPGIKPYHLTFSEGVPMEMSVNHQQNFTSDDTSKCEGSKCIPEVQTPPAFISNKHADFPFIPDLKIPAFKYNQDPSASGKHTPNSSSSFSVGHSSEVIVDAKKVFSAEKGHPNGMALDMLKEHSGSDKSSGGKHDLPLDLSEKSTRHELCSKSAISALQAALVVHQCPYCSHNTYYPEVLWMHKRIWHKVSCNSMAPQWIQQNGFKNLKNNLLFLTRSGRTGPPPALGGKECQPLPIARFSRTQMPIRPPTSKGNTANLSSVSKAACGSQVRDSPHGFSGAKTANFEVQRHQKVGDQYSAAVQQPLLKSKYETSPKLMQAASFSRNLSPAQASVSRQILQPSSSKQVEKYGMPQGGPSTFASPSKLSVSDTIKTKFPLQQPQYPFHKVEPQVKQESPPVPHRESQGKIVNELGTVSNCSTGMKTSPLFHSQPSTAGDPQSFQPVKQEPVSEGHEKRLDILNIFKTYIPKDLATLYQSWGANANNLDNAGMLRTQARQGDYVCNECGKCFSQPSHLRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQCVHRVPFDNSEYPDKQLLQPQSDDNSYMDDQLENLPSNHQVPGAVALK

Target ORF information:

RefSeq Version	XM_012964127.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964127.3

ORF Insert Sequence:

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGGCATTAAA CCATACCATC TTACATTTTC TGAGGGTGTA 
CCCATGGAAA TGTCAGTAAA TCATCAACAG AACTTCACTT CTGATGATAC CAGCAAATGT 
GAAGGAAGTA AATGTATTCC AGAGGTGCAG ACTCCTCCAG CCTTCATATC TAATAAGCAT 
GCTGATTTTC CTTTTATCCC TGATTTGAAA ATACCAGCAT TCAAGTACAA TCAAGATCCC 
AGTGCTTCTG GAAAGCATAC CCCGAACAGT AGTTCATCTT TTTCTGTGGG ACATAGTTCA 
GAAGTAATTG TGGATGCAAA AAAAGTTTTT TCAGCAGAAA AGGGTCACCC AAATGGAATG 
GCACTTGATA TGCTGAAGGA ACACAGTGGG TCAGATAAAA GCAGTGGTGG AAAACACGAC 
CTTCCTTTGG ATCTTAGTGA AAAATCTACT AGGCATGAAC TTTGTAGTAA AAGTGCTATT 
TCTGCATTGC AGGCAGCGTT GGTGGTTCAC CAGTGTCCAT ATTGCAGCCA CAATACTTAT 
TATCCTGAGG TGCTGTGGAT GCACAAAAGA ATTTGGCATA AAGTTAGTTG TAATTCAATG 
GCTCCTCAAT GGATTCAGCA AAATGGTTTC AAAAATTTGA AAAATAATTT GCTTTTCCTA 
ACTAGGAGTG GGCGTACAGG TCCGCCTCCA GCACTTGGTG GTAAAGAATG TCAGCCTTTA 
CCCATAGCTA GGTTCTCACG CACTCAAATG CCAATTCGGC CTCCAACATC CAAAGGAAAT 
ACTGCAAATC TAAGCAGTGT TTCCAAGGCA GCATGTGGAT CTCAGGTTCG TGACAGCCCT 
CATGGATTCA GTGGGGCGAA AACCGCAAAC TTTGAAGTCC AGAGGCACCA AAAGGTAGGG 
GACCAATATA GTGCTGCTGT ACAGCAACCA TTGTTAAAAT CTAAATATGA AACTAGTCCA 
AAATTGATGC AAGCTGCAAG TTTCAGTAGA AACCTTTCAC CTGCTCAAGC ATCAGTATCA 
AGACAAATAC TTCAGCCCTC TAGTAGCAAA CAAGTGGAGA AATACGGGAT GCCTCAAGGT 
GGACCTAGTA CATTTGCATC TCCGAGTAAA CTATCTGTAT CTGACACAAT TAAAACCAAA 
TTTCCTCTTC AGCAGCCACA GTACCCATTT CACAAAGTGG AGCCACAAGT TAAACAGGAG 
AGTCCTCCAG TGCCTCACCG GGAATCTCAG GGCAAGATTG TCAATGAACT AGGGACAGTG 
TCCAACTGTA GCACAGGCAT GAAGACAAGC CCTTTGTTTC ATTCTCAACC AAGTACAGCA 
GGTGACCCCC AATCTTTTCA ACCGGTCAAG CAAGAACCAG TATCTGAAGG CCATGAAAAA 
CGACTGGACA TTTTAAATAT CTTTAAGACC TACATTCCAA AGGATCTTGC TACTCTCTAC 
CAAAGCTGGG GTGCCAACGC TAATAATTTG GATAATGCAG GAATGCTTAG GACACAAGCA 
CGGCAAGGAG ACTATGTTTG CAATGAGTGC GGAAAATGTT TCAGCCAACC AAGCCACTTA 
AGAACCCATA TGAGGTCCCA TACAGGGGAG AGACCATTCC AGTGCAGATA TTGTCCATAC 
AGTGCCTCTC AAAAAGGGAA CCTAAAAACA CACGTACAGT GTGTACATCG GGTACCATTT 
GACAATAGTG AGTACCCTGA CAAACAGCTC CTTCAACCTC AAAGCGATGA TAACAGTTAC 
ATGGACGATC AGCTGGAAAA TTTGCCTTCA AATCACCAAG TCCCTGGAGC TGTGGCGTTA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_031903140.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759000.1 Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X2, mRNA.

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CloneID	OXa59449
Clone ID Related Accession (Same CDS sequence)	XM_012964127.3 , XM_031903140.1
Accession Version	XM_031903140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 516 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGGCATTAAA CCATACCATC TTACATTTTC TGAGGGTGTA 
CCCATGGAAA TGTCAGTAAA TCATCAACAG AACTTCACTT CTGATGATAC CAGCAAATGT 
GAAGGAAGTA AATGTATTCC AGAGGTGCAG ACTCCTCCAG CCTTCATATC TAATAAGCAT 
GCTGATTTTC CTTTTATCCC TGATTTGAAA ATACCAGCAT TCAAGTACAA TCAAGATCCC 
AGTGCTTCTG GAAAGCATAC CCCGAACAGT AGTTCATCTT TTTCTGTGGG ACATAGTTCA 
GAAGTAATTG TGGATGCAAA AAAAGTTTTT TCAGCAGAAA AGGGTCACCC AAATGGAATG 
GCACTTGATA TGCTGAAGGA ACACAGTGGG TCAGATAAAA GCAGTGGTGG AAAACACGAC 
CTTCCTTTGG ATCTTAGTGA AAAATCTACT AGGCATGAAC TTTGTAGTAA AAGTGCTATT 
TCTGCATTGC AGGCAGCGTT GGTGGTTCAC CAGTGTCCAT ATTGCAGCCA CAATACTTAT 
TATCCTGAGG TGCTGTGGAT GCACAAAAGA ATTTGGCATA AAGTTAGTTG TAATTCAATG 
GCTCCTCAAT GGATTCAGCA AAATGGTTTC AAAAATTTGA AAAATAATTT GCTTTTCCTA 
ACTAGGAGTG GGCGTACAGG TCCGCCTCCA GCACTTGGTG GTAAAGAATG TCAGCCTTTA 
CCCATAGCTA GGTTCTCACG CACTCAAATG CCAATTCGGC CTCCAACATC CAAAGGAAAT 
ACTGCAAATC TAAGCAGTGT TTCCAAGGCA GCATGTGGAT CTCAGGTTCG TGACAGCCCT 
CATGGATTCA GTGGGGCGAA AACCGCAAAC TTTGAAGTCC AGAGGCACCA AAAGGTAGGG 
GACCAATATA GTGCTGCTGT ACAGCAACCA TTGTTAAAAT CTAAATATGA AACTAGTCCA 
AAATTGATGC AAGCTGCAAG TTTCAGTAGA AACCTTTCAC CTGCTCAAGC ATCAGTATCA 
AGACAAATAC TTCAGCCCTC TAGTAGCAAA CAAGTGGAGA AATACGGGAT GCCTCAAGGT 
GGACCTAGTA CATTTGCATC TCCGAGTAAA CTATCTGTAT CTGACACAAT TAAAACCAAA 
TTTCCTCTTC AGCAGCCACA GTACCCATTT CACAAAGTGG AGCCACAAGT TAAACAGGAG 
AGTCCTCCAG TGCCTCACCG GGAATCTCAG GGCAAGATTG TCAATGAACT AGGGACAGTG 
TCCAACTGTA GCACAGGCAT GAAGACAAGC CCTTTGTTTC ATTCTCAACC AAGTACAGCA 
GGTGACCCCC AATCTTTTCA ACCGGTCAAG CAAGAACCAG TATCTGAAGG CCATGAAAAA 
CGACTGGACA TTTTAAATAT CTTTAAGACC TACATTCCAA AGGATCTTGC TACTCTCTAC 
CAAAGCTGGG GTGCCAACGC TAATAATTTG GATAATGCAG GAATGCTTAG GACACAAGCA 
CGGCAAGGAG ACTATGTTTG CAATGAGTGC GGAAAATGTT TCAGCCAACC AAGCCACTTA 
AGAACCCATA TGAGGTCCCA TACAGGGGAG AGACCATTCC AGTGCAGATA TTGTCCATAC 
AGTGCCTCTC AAAAAGGGAA CCTAAAAACA CACGTACAGT GTGTACATCG GGTACCATTT 
GACAATAGTG AGTACCCTGA CAAACAGCTC CTTCAACCTC AAAGCGATGA TAACAGTTAC 
ATGGACGATC AGCTGGAAAA TTTGCCTTCA AATCACCAAG TCCCTGGAGC TGTGGCGTTA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759000.1
CDS	798..4343
Translation	MERRKQFDMSLRESSAKSGKNDTEEDKGSGFSCVICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRAAQKGNLKIHLRTHRSGTINQVTDMEMGDLHVGEFGASEGMGGCASPTKSTSACNKILNGTLQTEEAEEVDDDQLGNFQCPFCKNKYDRKKELDQHLLQVHKPYKCRFCSYMTLREETLLNHIEKDHITTAVPLNRDGLAENGKTEINSGEFPCEVCGQAFGQAWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGNKNKPKSDSEPVATINDVVQEETIMTGLSLYEVCMKCGNLFTNLESLNAHNLVHCKTEEISLEDPIEQNRENDTPNSMGAKQFLLDYLHLRPLKDANKPSDIQQGKRVPELDPINSYQAWQLATKGKVAEPSEYVKYLGWDETMADADVTYDKDKGEYILVNQEKRKREPDSQSTSNPKKRNSSSVSRSEKSSNTQSGENVDGSNELEFRPPSRQSRRSSQNKSTECFECGKVFRTYHQMVLHSRVHRKDRRSLSESGSVSHNDRYGSNSDIDSASRPSSPDSASAPEDSLASGMGEDAADDDSFEEGPTPSPGIKPYHLTFSEGVPMEMSVNHQQNFTSDDTSKCEGSKCIPEVQTPPAFISNKHADFPFIPDLKIPAFKYNQDPSASGKHTPNSSSSFSVGHSSEVIVDAKKVFSAEKGHPNGMALDMLKEHSGSDKSSGGKHDLPLDLSEKSTRHELCSKSAISALQAALVVHQCPYCSHNTYYPEVLWMHKRIWHKVSCNSMAPQWIQQNGFKNLKNNLLFLTRSGRTGPPPALGGKECQPLPIARFSRTQMPIRPPTSKGNTANLSSVSKAACGSQVRDSPHGFSGAKTANFEVQRHQKVGDQYSAAVQQPLLKSKYETSPKLMQAASFSRNLSPAQASVSRQILQPSSSKQVEKYGMPQGGPSTFASPSKLSVSDTIKTKFPLQQPQYPFHKVEPQVKQESPPVPHRESQGKIVNELGTVSNCSTGMKTSPLFHSQPSTAGDPQSFQPVKQEPVSEGHEKRLDILNIFKTYIPKDLATLYQSWGANANNLDNAGMLRTQARQGDYVCNECGKCFSQPSHLRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQCVHRVPFDNSEYPDKQLLQPQSDDNSYMDDQLENLPSNHQVPGAVALK

Target ORF information:

RefSeq Version	XM_031903140.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903140.1

ORF Insert Sequence:

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGGCATTAAA CCATACCATC TTACATTTTC TGAGGGTGTA 
CCCATGGAAA TGTCAGTAAA TCATCAACAG AACTTCACTT CTGATGATAC CAGCAAATGT 
GAAGGAAGTA AATGTATTCC AGAGGTGCAG ACTCCTCCAG CCTTCATATC TAATAAGCAT 
GCTGATTTTC CTTTTATCCC TGATTTGAAA ATACCAGCAT TCAAGTACAA TCAAGATCCC 
AGTGCTTCTG GAAAGCATAC CCCGAACAGT AGTTCATCTT TTTCTGTGGG ACATAGTTCA 
GAAGTAATTG TGGATGCAAA AAAAGTTTTT TCAGCAGAAA AGGGTCACCC AAATGGAATG 
GCACTTGATA TGCTGAAGGA ACACAGTGGG TCAGATAAAA GCAGTGGTGG AAAACACGAC 
CTTCCTTTGG ATCTTAGTGA AAAATCTACT AGGCATGAAC TTTGTAGTAA AAGTGCTATT 
TCTGCATTGC AGGCAGCGTT GGTGGTTCAC CAGTGTCCAT ATTGCAGCCA CAATACTTAT 
TATCCTGAGG TGCTGTGGAT GCACAAAAGA ATTTGGCATA AAGTTAGTTG TAATTCAATG 
GCTCCTCAAT GGATTCAGCA AAATGGTTTC AAAAATTTGA AAAATAATTT GCTTTTCCTA 
ACTAGGAGTG GGCGTACAGG TCCGCCTCCA GCACTTGGTG GTAAAGAATG TCAGCCTTTA 
CCCATAGCTA GGTTCTCACG CACTCAAATG CCAATTCGGC CTCCAACATC CAAAGGAAAT 
ACTGCAAATC TAAGCAGTGT TTCCAAGGCA GCATGTGGAT CTCAGGTTCG TGACAGCCCT 
CATGGATTCA GTGGGGCGAA AACCGCAAAC TTTGAAGTCC AGAGGCACCA AAAGGTAGGG 
GACCAATATA GTGCTGCTGT ACAGCAACCA TTGTTAAAAT CTAAATATGA AACTAGTCCA 
AAATTGATGC AAGCTGCAAG TTTCAGTAGA AACCTTTCAC CTGCTCAAGC ATCAGTATCA 
AGACAAATAC TTCAGCCCTC TAGTAGCAAA CAAGTGGAGA AATACGGGAT GCCTCAAGGT 
GGACCTAGTA CATTTGCATC TCCGAGTAAA CTATCTGTAT CTGACACAAT TAAAACCAAA 
TTTCCTCTTC AGCAGCCACA GTACCCATTT CACAAAGTGG AGCCACAAGT TAAACAGGAG 
AGTCCTCCAG TGCCTCACCG GGAATCTCAG GGCAAGATTG TCAATGAACT AGGGACAGTG 
TCCAACTGTA GCACAGGCAT GAAGACAAGC CCTTTGTTTC ATTCTCAACC AAGTACAGCA 
GGTGACCCCC AATCTTTTCA ACCGGTCAAG CAAGAACCAG TATCTGAAGG CCATGAAAAA 
CGACTGGACA TTTTAAATAT CTTTAAGACC TACATTCCAA AGGATCTTGC TACTCTCTAC 
CAAAGCTGGG GTGCCAACGC TAATAATTTG GATAATGCAG GAATGCTTAG GACACAAGCA 
CGGCAAGGAG ACTATGTTTG CAATGAGTGC GGAAAATGTT TCAGCCAACC AAGCCACTTA 
AGAACCCATA TGAGGTCCCA TACAGGGGAG AGACCATTCC AGTGCAGATA TTGTCCATAC 
AGTGCCTCTC AAAAAGGGAA CCTAAAAACA CACGTACAGT GTGTACATCG GGTACCATTT 
GACAATAGTG AGTACCCTGA CAAACAGCTC CTTCAACCTC AAAGCGATGA TAACAGTTAC 
ATGGACGATC AGCTGGAAAA TTTGCCTTCA AATCACCAAG TCCCTGGAGC TGTGGCGTTA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_031903141.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759001.1 Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X3, mRNA.

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CloneID	OXa59450
Clone ID Related Accession (Same CDS sequence)	XM_031903141.1
Accession Version	XM_031903141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 516 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGGCATTAAA CCATACCATC TTACATTTTC TGAGGGTGTA 
CCCATGGAAA TGTCAGTAAA TCATCAACAG AACTTCACTT CTGATGATAC CAGCAAATGT 
GAAGGAAGTA AATGTATTCC AGAGGTGCAG ACTCCTCCAG CCTTCATATC TAATAAGCAT 
GCTGATTTTC CTTTTATCCC TGATTTGAAA ATACCAGCAT TCAAGTACAA TCAAGATCCC 
AGTGCTTCTG GAAAGCATAC CCCGAACAGT AGTTCATCTT TTTCTGTGGG ACATAGTTCA 
GAAGTAATTG TGGATGCAAA AAAAGTTTTT TCAGCAGAAA AGGGTCACCC AAATGGAATG 
GCACTTGATA TGCTGAAGGA ACACAGTGGG TCAGATAAAA GCAGTGGTGG AAAACACGAC 
CTTCCTTTGG ATCTTAGTGA AAAATCTACT AGGCATGAAC TTTGTAGTAA AAGTGCTATT 
TCTGCATTGC AGGCAGCGTT GGTGGTTCAC CAGTGTCCAT ATTGCAGCCA CAATACTTAT 
TATCCTGAGG TGCTGTGGAT GCACAAAAGA ATTTGGCATA AAGTTAGTTG TAATTCAATG 
GCTCCTCAAT GGATTCAGCA AAATGGTTTC AAAAATTTGA AAAATAATTT GCTTTTCCTA 
ACTAGGAGTG GGCGTACAGG TCCGCCTCCA GCACTTGGTG GTAAAGAATG TCAGCCTTTA 
CCCATAGCTA GGTTCTCACG CACTCAAATG CCAATTCGGC CTCCAACATC CAAAGGAAAT 
ACTGCAAATC TAAGCAGTGT TTCCAAGGCA GCATGTGGAT CTCAGGTTCG TGACAGCCCT 
CATGGATTCA GTGGGGCGAA AACCGCAAAC TTTGAAGTCC AGAGGCACCA AAAGGTAGGG 
GACCAATATA GTGCTGCTGT ACAGCAACCA TTGTTAAAAT CTAAATATGA AACTAGTCCA 
AAATTGATGC AAGCTGCAAG TTTCAGTAGA AACCTTTCAC CTGCTCAAGC ATCAGTATCA 
AGACAAATAC TTCAGCCCTC TAGTAGCAAA CAAGTGGAGA AATACGGGAT GCCTCAAGGT 
GGACCTAGTA CATTTGCATC TCCGAGTAAA CTATCTGTAT CTGACACAAT TAAAACCAAA 
TTTCCTCTTC AGCAGCCACA GTACCCATTT CACAAAGTGG AGCCACAAGT TAAACAGGAG 
AGTCCTCCAG TGCCTCACCG GGAATCTCAG GGCAAGATTG TCAATGAACT AGGGACAGTG 
TCCAACTGTA GCACAGGCAT GAAGACAAGC CCTTTGTTTC ATTCTCAACC AAGTACAGCA 
GGTGACCCCC AATCTTTTCA ACCGGTCAAG CAAGAACCAG TATCTGAAGG CCATGAAAAA 
CGACTGGACA TTTTAAATAT CTTTAAGACC TACATTCCAA AGGATCTTGC TACTCTCTAC 
CAAAGCTGGG GTGCCAACGC TAATAATTTG GATAATGCAG GAATGCTTAG GACACAAGCA 
CGGCAAGGAG ACTATGTTTG CAATGAGTGC GGAAAATGTT TCAGCCAACC AAGCCACTTA 
AGAACCCATA TGAGGTCCCA TACAGTGGTA TTTGAGTCTA ATGGACTCCG AGGTACTGAA 
GTTCACACCA CCTCCGCAGA TGCCCCAAAA CAAGGGAGAG ACCATTCCAG TGCAGATATT 
GTCCATACAG TGCCTCTCAA AAAGGGAACC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759001.1
CDS	713..4165
Translation	MERRKQFDMSLRESSAKSGKNDTEEDKGSGFSCVICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRAAQKGNLKIHLRTHRSGTINQVTDMEMGDLHVGEFGASEGMGGCASPTKSTSACNKILNGTLQTEEAEEVDDDQLGNFQCPFCKNKYDRKKELDQHLLQVHKPYKCRFCSYMTLREETLLNHIEKDHITTAVPLNRDGLAENGKTEINSGEFPCEVCGQAFGQAWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGNKNKPKSDSEPVATINDVVQEETIMTGLSLYEVCMKCGNLFTNLESLNAHNLVHCKTEEISLEDPIEQNRENDTPNSMGAKQFLLDYLHLRPLKDANKPSDIQQGKRVPELDPINSYQAWQLATKGKVAEPSEYVKYLGWDETMADADVTYDKDKGEYILVNQEKRKREPDSQSTSNPKKRNSSSVSRSEKSSNTQSGENVDGSNELEFRPPSRQSRRSSQNKSTECFECGKVFRTYHQMVLHSRVHRKDRRSLSESGSVSHNDRYGSNSDIDSASRPSSPDSASAPEDSLASGMGEDAADDDSFEEGPTPSPGIKPYHLTFSEGVPMEMSVNHQQNFTSDDTSKCEGSKCIPEVQTPPAFISNKHADFPFIPDLKIPAFKYNQDPSASGKHTPNSSSSFSVGHSSEVIVDAKKVFSAEKGHPNGMALDMLKEHSGSDKSSGGKHDLPLDLSEKSTRHELCSKSAISALQAALVVHQCPYCSHNTYYPEVLWMHKRIWHKVSCNSMAPQWIQQNGFKNLKNNLLFLTRSGRTGPPPALGGKECQPLPIARFSRTQMPIRPPTSKGNTANLSSVSKAACGSQVRDSPHGFSGAKTANFEVQRHQKVGDQYSAAVQQPLLKSKYETSPKLMQAASFSRNLSPAQASVSRQILQPSSSKQVEKYGMPQGGPSTFASPSKLSVSDTIKTKFPLQQPQYPFHKVEPQVKQESPPVPHRESQGKIVNELGTVSNCSTGMKTSPLFHSQPSTAGDPQSFQPVKQEPVSEGHEKRLDILNIFKTYIPKDLATLYQSWGANANNLDNAGMLRTQARQGDYVCNECGKCFSQPSHLRTHMRSHTVVFESNGLRGTEVHTTSADAPKQGRDHSSADIVHTVPLKKGT

Target ORF information:

RefSeq Version	XM_031903141.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903141.1

ORF Insert Sequence:

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGGCATTAAA CCATACCATC TTACATTTTC TGAGGGTGTA 
CCCATGGAAA TGTCAGTAAA TCATCAACAG AACTTCACTT CTGATGATAC CAGCAAATGT 
GAAGGAAGTA AATGTATTCC AGAGGTGCAG ACTCCTCCAG CCTTCATATC TAATAAGCAT 
GCTGATTTTC CTTTTATCCC TGATTTGAAA ATACCAGCAT TCAAGTACAA TCAAGATCCC 
AGTGCTTCTG GAAAGCATAC CCCGAACAGT AGTTCATCTT TTTCTGTGGG ACATAGTTCA 
GAAGTAATTG TGGATGCAAA AAAAGTTTTT TCAGCAGAAA AGGGTCACCC AAATGGAATG 
GCACTTGATA TGCTGAAGGA ACACAGTGGG TCAGATAAAA GCAGTGGTGG AAAACACGAC 
CTTCCTTTGG ATCTTAGTGA AAAATCTACT AGGCATGAAC TTTGTAGTAA AAGTGCTATT 
TCTGCATTGC AGGCAGCGTT GGTGGTTCAC CAGTGTCCAT ATTGCAGCCA CAATACTTAT 
TATCCTGAGG TGCTGTGGAT GCACAAAAGA ATTTGGCATA AAGTTAGTTG TAATTCAATG 
GCTCCTCAAT GGATTCAGCA AAATGGTTTC AAAAATTTGA AAAATAATTT GCTTTTCCTA 
ACTAGGAGTG GGCGTACAGG TCCGCCTCCA GCACTTGGTG GTAAAGAATG TCAGCCTTTA 
CCCATAGCTA GGTTCTCACG CACTCAAATG CCAATTCGGC CTCCAACATC CAAAGGAAAT 
ACTGCAAATC TAAGCAGTGT TTCCAAGGCA GCATGTGGAT CTCAGGTTCG TGACAGCCCT 
CATGGATTCA GTGGGGCGAA AACCGCAAAC TTTGAAGTCC AGAGGCACCA AAAGGTAGGG 
GACCAATATA GTGCTGCTGT ACAGCAACCA TTGTTAAAAT CTAAATATGA AACTAGTCCA 
AAATTGATGC AAGCTGCAAG TTTCAGTAGA AACCTTTCAC CTGCTCAAGC ATCAGTATCA 
AGACAAATAC TTCAGCCCTC TAGTAGCAAA CAAGTGGAGA AATACGGGAT GCCTCAAGGT 
GGACCTAGTA CATTTGCATC TCCGAGTAAA CTATCTGTAT CTGACACAAT TAAAACCAAA 
TTTCCTCTTC AGCAGCCACA GTACCCATTT CACAAAGTGG AGCCACAAGT TAAACAGGAG 
AGTCCTCCAG TGCCTCACCG GGAATCTCAG GGCAAGATTG TCAATGAACT AGGGACAGTG 
TCCAACTGTA GCACAGGCAT GAAGACAAGC CCTTTGTTTC ATTCTCAACC AAGTACAGCA 
GGTGACCCCC AATCTTTTCA ACCGGTCAAG CAAGAACCAG TATCTGAAGG CCATGAAAAA 
CGACTGGACA TTTTAAATAT CTTTAAGACC TACATTCCAA AGGATCTTGC TACTCTCTAC 
CAAAGCTGGG GTGCCAACGC TAATAATTTG GATAATGCAG GAATGCTTAG GACACAAGCA 
CGGCAAGGAG ACTATGTTTG CAATGAGTGC GGAAAATGTT TCAGCCAACC AAGCCACTTA 
AGAACCCATA TGAGGTCCCA TACAGTGGTA TTTGAGTCTA ATGGACTCCG AGGTACTGAA 
GTTCACACCA CCTCCGCAGA TGCCCCAAAA CAAGGGAGAG ACCATTCCAG TGCAGATATT 
GTCCATACAG TGCCTCTCAA AAAGGGAACC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( NM_001097250.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001090719.1 Xenopus tropicalis zinc finger protein 516 (znf516), mRNA.

Price & Availability↑

CloneID	OXa23389
Clone ID Related Accession (Same CDS sequence)	NM_001097250.1 , XM_012964127.2
Accession Version	NM_001097250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 516
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC128653.1. ##Evidence-Data-START## Transcript exon combination :: BC128653.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028292, SAMD00028293 [ECO:0000348] ##Evidence-Data-END##

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGGCATTAAA CCATACCATC TTACATTTTC TGAGGGTGTA 
CCCATGGAAA TGTCAGTAAA TCATCAACAG AACTTCACTT CTGATGATAC CAGCAAATGT 
GAAGGAAGTA AATGTATTCC AGAGGTGCAG ACTCCTCCAG CCTTCATATC TAATAAGCAT 
GCTGATTTTC CTTTTATCCC TGATTTGAAA ATACCAGCAT TCAAGTACAA TCAAGATCCC 
AGTGCTTCTG GAAAGCATAC CCCGAACAGT AGTTCATCTT TTTCTGTGGG ACATAGTTCA 
GAAGTAATTG TGGATGCAAA AAAAGTTTTT TCAGCAGAAA AGGGTCACCC AAATGGAATG 
GCACTTGATA TGCTGAAGGA ACACAGTGGG TCAGATAAAA GCAGTGGTGG AAAACACGAC 
CTTCCTTTGG ATCTTAGTGA AAAATCTACT AGGCATGAAC TTTGTAGTAA AAGTGCTATT 
TCTGCATTGC AGGCAGCGTT GGTGGTTCAC CAGTGTCCAT ATTGCAGCCA CAATACTTAT 
TATCCTGAGG TGCTGTGGAT GCACAAAAGA ATTTGGCATA AAGTTAGTTG TAATTCAATG 
GCTCCTCAAT GGATTCAGCA AAATGGTTTC AAAAATTTGA AAAATAATTT GCTTTTCCTA 
ACTAGGAGTG GGCGTACAGG TCCGCCTCCA GCACTTGGTG GTAAAGAATG TCAGCCTTTA 
CCCATAGCTA GGTTCTCACG CACTCAAATG CCAATTCGGC CTCCAACATC CAAAGGAAAT 
ACTGCAAATC TAAGCAGTGT TTCCAAGGCA GCATGTGGAT CTCAGGTTCG TGACAGCCCT 
CATGGATTCA GTGGGGCGAA AACCACAAAC TTTGAAGTCC AGAGGCACCA AAAGGTAGGG 
GACCAATATA GTGCTGCTGT ACAGCAACCA TTGTTAAAAT CTAAATATGA AACTAGTCCA 
AAATTGATGC AAGCTGCAAG TTTCAGTAGA AACCTTTCAC CTGCTCAAGC ATCAGTATCA 
AGACAAATAC TTCAGCCCTC TAGTAGCAAA CAAGTGGAGA AATACGGGAT GCCTCAAGGT 
GGACCTAGTA CATTTGCATC TCCGAGTAAA CTATCTGTAT CTGACACAAT TAAAACCAAA 
TTTCCTCTTC AGCAGCCACA GTACCCATTT CACAAAGTGG AGCCACAAGT TAAACAGGAG 
AGTCCTCCAG TGCCTCACCG GGAATCTCAG GGCAAGATTG TCAATGAACT AGGGACAGTG 
TCCAACTGTA GCACAGGCAT GAAGACAAGC CCTTTGTTTC ATTCTCAACC AAGTACAGCA 
GGTGACCCCC AATCTTTTCA ACCGGTCAAG CAAGAACCAG TATCTGAAGG CCATGAAAAA 
CGACTGGACA TTTTAAATAT CTTTAAGACC TACATTCCAA AGGATCTTGC TACTCTCTAC 
CAAAGCTGGG GTGCCAACGC TAATAATTTG GATAATGCAG GAATGCTTAG GACACAAGCA 
CGGCAAGGAG ACTATGTTTG CAATGAGTGC GGAAAATGTT TCAGCCAACC AAGCCACTTA 
AGAACCCATA TGAGGTCCCA TACAGGGGAG AGACCATTCC AGTGCAGATA TTGTCCATAC 
AGTGCCTCTC AAAAAGGGAA CCTAAAAACA CACGTACAGT GTGTACATCG GGTACCATTT 
GACAATAGTG AGTACCCTGA CAAACAGCTC CTTCAACCTC AAAGCGATGA TAACAGTTAC 
ATGGACGATC AGCTGGAAAA TTTGCCTTCA AATCACCAAG TCCCTGGAGC TGTGGCGTTA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001090719.1
CDS	316..3861
Translation	MERRKQFDMSLRESSAKSGKNDTEEDKGSGFSCVICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRAAQKGNLKIHLRTHRSGTINQVTDMEMGDLHVGEFGASEGMGGCASPTKSTSACNKILNGTLQTEEAEEVDDDQLGNFQCPFCKNKYDRKKELDQHLLQVHKPYKCRFCSYMTLREETLLNHIEKDHITTAVPLNRDGLAENGKTEINSGEFPCEVCGQAFGQAWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGNKNKPKSDSEPVATINDVVQEETIMTGLSLYEVCMKCGNLFTNLESLNAHNLVHCKTEEISLEDPIEQNRENDTPNSMGAKQFLLDYLHLRPLKDANKPSDIQQGKRVPELDPINSYQAWQLATKGKVAEPSEYVKYLGWDETMADADVTYDKDKGEYILVNQEKRKREPDSQSTSNPKKRNSSSVSRSEKSSNTQSGENVDGSNELEFRPPSRQSRRSSQNKSTECFECGKVFRTYHQMVLHSRVHRKDRRSLSESGSVSHNDRYGSNSDIDSASRPSSPDSASAPEDSLASGMGEDAADDDSFEEGPTPSPGIKPYHLTFSEGVPMEMSVNHQQNFTSDDTSKCEGSKCIPEVQTPPAFISNKHADFPFIPDLKIPAFKYNQDPSASGKHTPNSSSSFSVGHSSEVIVDAKKVFSAEKGHPNGMALDMLKEHSGSDKSSGGKHDLPLDLSEKSTRHELCSKSAISALQAALVVHQCPYCSHNTYYPEVLWMHKRIWHKVSCNSMAPQWIQQNGFKNLKNNLLFLTRSGRTGPPPALGGKECQPLPIARFSRTQMPIRPPTSKGNTANLSSVSKAACGSQVRDSPHGFSGAKTTNFEVQRHQKVGDQYSAAVQQPLLKSKYETSPKLMQAASFSRNLSPAQASVSRQILQPSSSKQVEKYGMPQGGPSTFASPSKLSVSDTIKTKFPLQQPQYPFHKVEPQVKQESPPVPHRESQGKIVNELGTVSNCSTGMKTSPLFHSQPSTAGDPQSFQPVKQEPVSEGHEKRLDILNIFKTYIPKDLATLYQSWGANANNLDNAGMLRTQARQGDYVCNECGKCFSQPSHLRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQCVHRVPFDNSEYPDKQLLQPQSDDNSYMDDQLENLPSNHQVPGAVALK

Target ORF information:

RefSeq Version	NM_001097250.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 516 (znf516), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097250.1

ORF Insert Sequence:

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGGCATTAAA CCATACCATC TTACATTTTC TGAGGGTGTA 
CCCATGGAAA TGTCAGTAAA TCATCAACAG AACTTCACTT CTGATGATAC CAGCAAATGT 
GAAGGAAGTA AATGTATTCC AGAGGTGCAG ACTCCTCCAG CCTTCATATC TAATAAGCAT 
GCTGATTTTC CTTTTATCCC TGATTTGAAA ATACCAGCAT TCAAGTACAA TCAAGATCCC 
AGTGCTTCTG GAAAGCATAC CCCGAACAGT AGTTCATCTT TTTCTGTGGG ACATAGTTCA 
GAAGTAATTG TGGATGCAAA AAAAGTTTTT TCAGCAGAAA AGGGTCACCC AAATGGAATG 
GCACTTGATA TGCTGAAGGA ACACAGTGGG TCAGATAAAA GCAGTGGTGG AAAACACGAC 
CTTCCTTTGG ATCTTAGTGA AAAATCTACT AGGCATGAAC TTTGTAGTAA AAGTGCTATT 
TCTGCATTGC AGGCAGCGTT GGTGGTTCAC CAGTGTCCAT ATTGCAGCCA CAATACTTAT 
TATCCTGAGG TGCTGTGGAT GCACAAAAGA ATTTGGCATA AAGTTAGTTG TAATTCAATG 
GCTCCTCAAT GGATTCAGCA AAATGGTTTC AAAAATTTGA AAAATAATTT GCTTTTCCTA 
ACTAGGAGTG GGCGTACAGG TCCGCCTCCA GCACTTGGTG GTAAAGAATG TCAGCCTTTA 
CCCATAGCTA GGTTCTCACG CACTCAAATG CCAATTCGGC CTCCAACATC CAAAGGAAAT 
ACTGCAAATC TAAGCAGTGT TTCCAAGGCA GCATGTGGAT CTCAGGTTCG TGACAGCCCT 
CATGGATTCA GTGGGGCGAA AACCACAAAC TTTGAAGTCC AGAGGCACCA AAAGGTAGGG 
GACCAATATA GTGCTGCTGT ACAGCAACCA TTGTTAAAAT CTAAATATGA AACTAGTCCA 
AAATTGATGC AAGCTGCAAG TTTCAGTAGA AACCTTTCAC CTGCTCAAGC ATCAGTATCA 
AGACAAATAC TTCAGCCCTC TAGTAGCAAA CAAGTGGAGA AATACGGGAT GCCTCAAGGT 
GGACCTAGTA CATTTGCATC TCCGAGTAAA CTATCTGTAT CTGACACAAT TAAAACCAAA 
TTTCCTCTTC AGCAGCCACA GTACCCATTT CACAAAGTGG AGCCACAAGT TAAACAGGAG 
AGTCCTCCAG TGCCTCACCG GGAATCTCAG GGCAAGATTG TCAATGAACT AGGGACAGTG 
TCCAACTGTA GCACAGGCAT GAAGACAAGC CCTTTGTTTC ATTCTCAACC AAGTACAGCA 
GGTGACCCCC AATCTTTTCA ACCGGTCAAG CAAGAACCAG TATCTGAAGG CCATGAAAAA 
CGACTGGACA TTTTAAATAT CTTTAAGACC TACATTCCAA AGGATCTTGC TACTCTCTAC 
CAAAGCTGGG GTGCCAACGC TAATAATTTG GATAATGCAG GAATGCTTAG GACACAAGCA 
CGGCAAGGAG ACTATGTTTG CAATGAGTGC GGAAAATGTT TCAGCCAACC AAGCCACTTA 
AGAACCCATA TGAGGTCCCA TACAGGGGAG AGACCATTCC AGTGCAGATA TTGTCCATAC 
AGTGCCTCTC AAAAAGGGAA CCTAAAAACA CACGTACAGT GTGTACATCG GGTACCATTT 
GACAATAGTG AGTACCCTGA CAAACAGCTC CTTCAACCTC AAAGCGATGA TAACAGTTAC 
ATGGACGATC AGCTGGAAAA TTTGCCTTCA AATCACCAAG TCCCTGGAGC TGTGGCGTTA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_012964127.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819581.1 Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X7, mRNA.

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CloneID	OXa23389
Clone ID Related Accession (Same CDS sequence)	NM_001097250.1 , XM_012964127.2
Accession Version	XM_012964127.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 516 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683375.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964127.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGGCATTAAA CCATACCATC TTACATTTTC TGAGGGTGTA 
CCCATGGAAA TGTCAGTAAA TCATCAACAG AACTTCACTT CTGATGATAC CAGCAAATGT 
GAAGGAAGTA AATGTATTCC AGAGGTGCAG ACTCCTCCAG CCTTCATATC TAATAAGCAT 
GCTGATTTTC CTTTTATCCC TGATTTGAAA ATACCAGCAT TCAAGTACAA TCAAGATCCC 
AGTGCTTCTG GAAAGCATAC CCCGAACAGT AGTTCATCTT TTTCTGTGGG ACATAGTTCA 
GAAGTAATTG TGGATGCAAA AAAAGTTTTT TCAGCAGAAA AGGGTCACCC AAATGGAATG 
GCACTTGATA TGCTGAAGGA ACACAGTGGG TCAGATAAAA GCAGTGGTGG AAAACACGAC 
CTTCCTTTGG ATCTTAGTGA AAAATCTACT AGGCATGAAC TTTGTAGTAA AAGTGCTATT 
TCTGCATTGC AGGCAGCGTT GGTGGTTCAC CAGTGTCCAT ATTGCAGCCA CAATACTTAT 
TATCCTGAGG TGCTGTGGAT GCACAAAAGA ATTTGGCATA AAGTTAGTTG TAATTCAATG 
GCTCCTCAAT GGATTCAGCA AAATGGTTTC AAAAATTTGA AAAATAATTT GCTTTTCCTA 
ACTAGGAGTG GGCGTACAGG TCCGCCTCCA GCACTTGGTG GTAAAGAATG TCAGCCTTTA 
CCCATAGCTA GGTTCTCACG CACTCAAATG CCAATTCGGC CTCCAACATC CAAAGGAAAT 
ACTGCAAATC TAAGCAGTGT TTCCAAGGCA GCATGTGGAT CTCAGGTTCG TGACAGCCCT 
CATGGATTCA GTGGGGCGAA AACCACAAAC TTTGAAGTCC AGAGGCACCA AAAGGTAGGG 
GACCAATATA GTGCTGCTGT ACAGCAACCA TTGTTAAAAT CTAAATATGA AACTAGTCCA 
AAATTGATGC AAGCTGCAAG TTTCAGTAGA AACCTTTCAC CTGCTCAAGC ATCAGTATCA 
AGACAAATAC TTCAGCCCTC TAGTAGCAAA CAAGTGGAGA AATACGGGAT GCCTCAAGGT 
GGACCTAGTA CATTTGCATC TCCGAGTAAA CTATCTGTAT CTGACACAAT TAAAACCAAA 
TTTCCTCTTC AGCAGCCACA GTACCCATTT CACAAAGTGG AGCCACAAGT TAAACAGGAG 
AGTCCTCCAG TGCCTCACCG GGAATCTCAG GGCAAGATTG TCAATGAACT AGGGACAGTG 
TCCAACTGTA GCACAGGCAT GAAGACAAGC CCTTTGTTTC ATTCTCAACC AAGTACAGCA 
GGTGACCCCC AATCTTTTCA ACCGGTCAAG CAAGAACCAG TATCTGAAGG CCATGAAAAA 
CGACTGGACA TTTTAAATAT CTTTAAGACC TACATTCCAA AGGATCTTGC TACTCTCTAC 
CAAAGCTGGG GTGCCAACGC TAATAATTTG GATAATGCAG GAATGCTTAG GACACAAGCA 
CGGCAAGGAG ACTATGTTTG CAATGAGTGC GGAAAATGTT TCAGCCAACC AAGCCACTTA 
AGAACCCATA TGAGGTCCCA TACAGGGGAG AGACCATTCC AGTGCAGATA TTGTCCATAC 
AGTGCCTCTC AAAAAGGGAA CCTAAAAACA CACGTACAGT GTGTACATCG GGTACCATTT 
GACAATAGTG AGTACCCTGA CAAACAGCTC CTTCAACCTC AAAGCGATGA TAACAGTTAC 
ATGGACGATC AGCTGGAAAA TTTGCCTTCA AATCACCAAG TCCCTGGAGC TGTGGCGTTA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819581.1
CDS	1052..4597
Translation	MERRKQFDMSLRESSAKSGKNDTEEDKGSGFSCVICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRAAQKGNLKIHLRTHRSGTINQVTDMEMGDLHVGEFGASEGMGGCASPTKSTSACNKILNGTLQTEEAEEVDDDQLGNFQCPFCKNKYDRKKELDQHLLQVHKPYKCRFCSYMTLREETLLNHIEKDHITTAVPLNRDGLAENGKTEINSGEFPCEVCGQAFGQAWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGNKNKPKSDSEPVATINDVVQEETIMTGLSLYEVCMKCGNLFTNLESLNAHNLVHCKTEEISLEDPIEQNRENDTPNSMGAKQFLLDYLHLRPLKDANKPSDIQQGKRVPELDPINSYQAWQLATKGKVAEPSEYVKYLGWDETMADADVTYDKDKGEYILVNQEKRKREPDSQSTSNPKKRNSSSVSRSEKSSNTQSGENVDGSNELEFRPPSRQSRRSSQNKSTECFECGKVFRTYHQMVLHSRVHRKDRRSLSESGSVSHNDRYGSNSDIDSASRPSSPDSASAPEDSLASGMGEDAADDDSFEEGPTPSPGIKPYHLTFSEGVPMEMSVNHQQNFTSDDTSKCEGSKCIPEVQTPPAFISNKHADFPFIPDLKIPAFKYNQDPSASGKHTPNSSSSFSVGHSSEVIVDAKKVFSAEKGHPNGMALDMLKEHSGSDKSSGGKHDLPLDLSEKSTRHELCSKSAISALQAALVVHQCPYCSHNTYYPEVLWMHKRIWHKVSCNSMAPQWIQQNGFKNLKNNLLFLTRSGRTGPPPALGGKECQPLPIARFSRTQMPIRPPTSKGNTANLSSVSKAACGSQVRDSPHGFSGAKTTNFEVQRHQKVGDQYSAAVQQPLLKSKYETSPKLMQAASFSRNLSPAQASVSRQILQPSSSKQVEKYGMPQGGPSTFASPSKLSVSDTIKTKFPLQQPQYPFHKVEPQVKQESPPVPHRESQGKIVNELGTVSNCSTGMKTSPLFHSQPSTAGDPQSFQPVKQEPVSEGHEKRLDILNIFKTYIPKDLATLYQSWGANANNLDNAGMLRTQARQGDYVCNECGKCFSQPSHLRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQCVHRVPFDNSEYPDKQLLQPQSDDNSYMDDQLENLPSNHQVPGAVALK

Target ORF information:

RefSeq Version	XM_012964127.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 516 (znf516), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964127.2

ORF Insert Sequence:

ATGGAACGTA GGAAACAATT TGACATGTCA CTACGTGAGT CCTCTGCAAA GTCAGGCAAA 
AATGATACAG AAGAAGACAA AGGATCAGGC TTCAGCTGTG TCATCTGTGG TAAAAGCTTT 
CCTTTCCAGA GCTCCCTTTC ACAACACATG CGAAAGCACA CTGGTGAAAA GCCATACAAA 
TGTCCTTACT GCGATCATCG GGCTGCCCAG AAAGGAAACT TGAAAATACA TCTAAGAACC 
CATAGAAGTG GCACAATTAA CCAGGTAACT GATATGGAAA TGGGAGATTT ACACGTTGGA 
GAATTTGGTG CTTCTGAGGG CATGGGAGGT TGTGCAAGCC CTACTAAAAG CACATCAGCT 
TGCAACAAAA TACTTAATGG CACCTTACAA ACAGAAGAGG CTGAAGAAGT GGATGATGAC 
CAACTCGGCA ACTTCCAGTG CCCATTTTGC AAAAACAAAT ATGACCGAAA GAAAGAACTT 
GATCAACATC TTTTACAAGT GCACAAACCC TATAAATGTA GATTTTGTAG CTACATGACT 
TTAAGAGAGG AGACTCTGTT AAATCATATA GAAAAAGACC ATATCACTAC AGCTGTACCA 
TTGAACAGAG ATGGTCTGGC AGAGAATGGC AAGACTGAAA TCAATTCAGG GGAGTTCCCT 
TGTGAAGTTT GTGGTCAAGC TTTTGGCCAA GCATGGTTCC TTAAAGCTCA TATGAAAAAG 
CACCGAGGAT CATTTGATCA TGGCTGCCAT ATATGTGGTA GAAGATTTAA AGAGCCATGG 
TTTCTCAAGA ACCACATGAA GTCTCATGGT CCAAAAACTG GGAACAAAAA CAAACCTAAG 
TCTGACTCTG AGCCAGTAGC TACTATTAAT GATGTTGTTC AAGAAGAGAC CATTATGACT 
GGCTTAAGCC TTTATGAAGT TTGCATGAAA TGTGGGAATT TGTTTACAAA TTTAGAAAGC 
CTAAATGCAC ACAACTTAGT GCACTGTAAA ACTGAAGAAA TCTCTCTTGA AGACCCAATA 
GAGCAAAATA GAGAAAATGA CACCCCAAAT TCAATGGGGG CTAAGCAGTT TTTACTTGAC 
TATCTTCATT TAAGACCTTT AAAAGATGCT AATAAGCCTT CCGATATACA ACAAGGTAAA 
AGAGTTCCAG AACTTGACCC AATCAACAGC TACCAAGCAT GGCAATTGGC TACAAAAGGA 
AAAGTTGCAG AGCCCTCAGA ATATGTTAAA TATCTGGGAT GGGATGAAAC CATGGCAGAT 
GCTGATGTAA CTTATGATAA AGACAAAGGA GAATACATCC TTGTTAATCA GGAGAAGCGA 
AAACGAGAGC CAGACAGTCA AAGTACCAGC AACCCAAAGA AACGCAACTC CTCAAGTGTT 
AGCCGATCGG AAAAGAGTAG CAATACACAA TCGGGAGAAA ATGTAGATGG TTCCAATGAA 
TTGGAGTTTA GGCCTCCTTC TCGTCAGAGC AGGCGTTCCT CTCAAAATAA ATCCACTGAA 
TGCTTTGAAT GTGGAAAAGT GTTCCGCACT TACCATCAAA TGGTGCTTCA TTCCAGGGTG 
CATAGAAAAG ACCGCAGGAG CCTCAGTGAA AGTGGGTCGG TTTCTCACAA TGATCGATAT 
GGGTCAAACA GTGACATTGA CTCTGCCAGC CGGCCCAGTA GTCCAGACTC TGCCTCTGCC 
CCAGAAGATT CTCTTGCCTC TGGTATGGGA GAAGATGCTG CTGATGACGA CAGTTTTGAA 
GAAGGTCCGA CTCCTTCACC AGGCATTAAA CCATACCATC TTACATTTTC TGAGGGTGTA 
CCCATGGAAA TGTCAGTAAA TCATCAACAG AACTTCACTT CTGATGATAC CAGCAAATGT 
GAAGGAAGTA AATGTATTCC AGAGGTGCAG ACTCCTCCAG CCTTCATATC TAATAAGCAT 
GCTGATTTTC CTTTTATCCC TGATTTGAAA ATACCAGCAT TCAAGTACAA TCAAGATCCC 
AGTGCTTCTG GAAAGCATAC CCCGAACAGT AGTTCATCTT TTTCTGTGGG ACATAGTTCA 
GAAGTAATTG TGGATGCAAA AAAAGTTTTT TCAGCAGAAA AGGGTCACCC AAATGGAATG 
GCACTTGATA TGCTGAAGGA ACACAGTGGG TCAGATAAAA GCAGTGGTGG AAAACACGAC 
CTTCCTTTGG ATCTTAGTGA AAAATCTACT AGGCATGAAC TTTGTAGTAA AAGTGCTATT 
TCTGCATTGC AGGCAGCGTT GGTGGTTCAC CAGTGTCCAT ATTGCAGCCA CAATACTTAT 
TATCCTGAGG TGCTGTGGAT GCACAAAAGA ATTTGGCATA AAGTTAGTTG TAATTCAATG 
GCTCCTCAAT GGATTCAGCA AAATGGTTTC AAAAATTTGA AAAATAATTT GCTTTTCCTA 
ACTAGGAGTG GGCGTACAGG TCCGCCTCCA GCACTTGGTG GTAAAGAATG TCAGCCTTTA 
CCCATAGCTA GGTTCTCACG CACTCAAATG CCAATTCGGC CTCCAACATC CAAAGGAAAT 
ACTGCAAATC TAAGCAGTGT TTCCAAGGCA GCATGTGGAT CTCAGGTTCG TGACAGCCCT 
CATGGATTCA GTGGGGCGAA AACCACAAAC TTTGAAGTCC AGAGGCACCA AAAGGTAGGG 
GACCAATATA GTGCTGCTGT ACAGCAACCA TTGTTAAAAT CTAAATATGA AACTAGTCCA 
AAATTGATGC AAGCTGCAAG TTTCAGTAGA AACCTTTCAC CTGCTCAAGC ATCAGTATCA 
AGACAAATAC TTCAGCCCTC TAGTAGCAAA CAAGTGGAGA AATACGGGAT GCCTCAAGGT 
GGACCTAGTA CATTTGCATC TCCGAGTAAA CTATCTGTAT CTGACACAAT TAAAACCAAA 
TTTCCTCTTC AGCAGCCACA GTACCCATTT CACAAAGTGG AGCCACAAGT TAAACAGGAG 
AGTCCTCCAG TGCCTCACCG GGAATCTCAG GGCAAGATTG TCAATGAACT AGGGACAGTG 
TCCAACTGTA GCACAGGCAT GAAGACAAGC CCTTTGTTTC ATTCTCAACC AAGTACAGCA 
GGTGACCCCC AATCTTTTCA ACCGGTCAAG CAAGAACCAG TATCTGAAGG CCATGAAAAA 
CGACTGGACA TTTTAAATAT CTTTAAGACC TACATTCCAA AGGATCTTGC TACTCTCTAC 
CAAAGCTGGG GTGCCAACGC TAATAATTTG GATAATGCAG GAATGCTTAG GACACAAGCA 
CGGCAAGGAG ACTATGTTTG CAATGAGTGC GGAAAATGTT TCAGCCAACC AAGCCACTTA 
AGAACCCATA TGAGGTCCCA TACAGGGGAG AGACCATTCC AGTGCAGATA TTGTCCATAC 
AGTGCCTCTC AAAAAGGGAA CCTAAAAACA CACGTACAGT GTGTACATCG GGTACCATTT 
GACAATAGTG AGTACCCTGA CAAACAGCTC CTTCAACCTC AAAGCGATGA TAACAGTTAC 
ATGGACGATC AGCTGGAAAA TTTGCCTTCA AATCACCAAG TCCCTGGAGC TGTGGCGTTA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	znf516
Entrez Gene ID	100036701
Full Name	zinc finger protein 516
General protein information	Preferred Names zinc finger protein 516 Names zinc finger protein 516
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Homo sapiens (human)	ZNF516	NP_055458.1
Bos taurus (cattle)	ZNF516	XP_002697699.2
Danio rerio (zebrafish)	LOC100003866	NP_001153141.1
Xenopus tropicalis (tropical clawed frog)	LOC100036701	NP_001090719.1
	ZNF516	XP_001095926.2
Canis lupus familiaris (dog)	ZNF516	XP_005615361.1
Rattus norvegicus (Norway rat)	Zfp516	NP_001178624.1
Pan troglodytes (chimpanzee)	ZNF516	XP_003315994.1
Gallus gallus (chicken)	ZNF516	XP_419095.4
Mus musculus (house mouse)	Zfp516	NP_001170935.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

znf516 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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