wnk1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following wnk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa23040 XM_031898630.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa54267 XM_031898626.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa54268 XM_031898627.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa54269 XM_031898628.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa54270 XM_031898629.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa54272 XM_031898632.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa23040 NM_001097234.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa54257 XM_031898616.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54258 XM_031898617.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54259 XM_031898618.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54260 XM_031898619.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54261 XM_031898620.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54262 XM_031898621.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54263 XM_031898622.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54264 XM_031898623.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54265 XM_031898624.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54266 XM_031898625.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54271 XM_031898631.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54273 XM_031898633.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa23040
    Clone ID Related Accession (Same CDS sequence) NM_001097234.1 , XM_031898630.1
    Accession Version XM_031898630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6309bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCACAAATA TCATCTGGAC AACCACCCAC CACATCCATG
    GGAATCAGTG CTCAGCCCGG TCCCCACACC CAGGGCATGG TGCATCATCA GCCTTCTCTG
    CATCCAGCCT CGCAGTACCA AGTGCAGCAG CCAATGCCTA CTGTGGGTTC CAGTATTGCG
    GCCCCATCAC AGCCGGTGAT TACCCAAACG CCAGCCCCTC AGCCGGGCAC CCAGGTTTTC
    CAGGCTACTC ACCCTCTATT CCCTGGAGAT GTAAGCTTTG TTCCTCCCGT TGCCATCCCC
    TCAGCAATGG TATCTCCCCC TATCCGTGGT GAAAGTTTAG CCCAACCTGG CTTCCATACT
    CAGCCTTATC CTGAAAACAG CAATTTGTAT TCCCATGGAG GGCCCACTCC CCAGCCCGCT
    GTTCTCACTA CCCAGCCGGT GCCTGCAGGA ACCCAGACGC AACCTGCCGT CGAGGGTTCA
    CAGAGTAGCT CCCTGGCAAT TTCCCAGGAT ACCCAGTCAT CTCCTCAAGC AACACAAACT
    GCCTCCTCCA TGGACAGCGC TCATTCAGAC GTGGCATCAG GACTGAGTGA CGGAAACGAG
    GGCAGCACAG GTGGGAGACA CGAAGGCCGA GCCACTAAAC GACACTACCG AAAATCTGTG
    AGAAGCCGCT CCAGGCACGA GAAAACTGCA CGCCCAAAGC TTCGAATCCT TAATGTTTCC
    AACAAAGGGG ATCGTGTTGT AGAGTGTCAG CTGGAGACTC ACAACCGCAA GATGGTCACC
    TTCAAATTCG ACCTGGATGG AGACAATCCA GAGGAGATTG CTACTATAAT GGTACAGAAT
    GTGTTTATTT TGGCTACGGA GAGAGAATCC TTTGTAGAGC AGGTAAAAGA CATAATTGAG
    AAGGCCGATG AAATGTTGAG CGAAGATGTG AGCGTGGAGC CGGAAGGAGA AACGAGGGCA
    GAAAGTTTGC AAATCAAGGA TGATGGGGAT TACCCAGAAT CTCAGAAGAT GAATTCTGAA
    TTTCGAATGC CAGATCCTGT ATATTCCCTA CCACAGACAG CAGGGCTTAT TCCCAGCGTT
    GTCACACAAG TCGTTCATTC TGCCGGAAGA CGATTTATTG TCAGTCCGGT GCCTGAAAGC
    CGTCTAAAAG AACAGTCATT CTTCAGTACT TCATTGCAGG ACCCTCAGCT ACCAGTCTCT
    GTCGCGCATG GTCCAGGGAT GAATTTGTCT CATTCTGCCT CTTCTGTGAG CCTGCAGCAA
    GCCTTTGCAG AACTACGCCA GTCACAGATG AGCGAGGGTC CTAGCACAGC TCCTCCCATA
    TTTAACCAAA CTGGACCCCC CTTTCCACCA TCCATGACTA GTCTGGTGGC AACTCTGCCT
    TCTAATGCAA TGCCTATCCT CCAGGCAGCC CCAGTGATCC CCAGTAGCCT GCCATCTTCT
    GTGCCTCAGC CCCTGGTGGA GGTGGTTGTA CCTCAGGCTG CTGGGATGGC TCCAACTAGC
    AATGTTCCAA GCACCTTGTC CCCTCCCCTA ACTGCAGTAT CCAACCAGCA CGCAGACATG
    ATACCGGGTG CTGCTGTAGC AGCTATCGGA TCACTTCCTG TGACATCACT TCCTCCTTTG
    CCCATTGTGG GTGGAGTTCC TGTAGGCACT GCAACCCTGA GCTCACCCCT AACACCCCTA
    ATCAGTTCTG TCAGTACAAT GGGGGGGACG GTTCCCCCAT CTGTCACATC TCCCCCAGTG
    CCACAGATAG CGGGTAGCGT TGCCGGGAGC CTGCCTCCTG CATCAGCCTC CGCCCCTCTT
    ATTCATGGCA GTGTTGCCCC ATCTCAGGTA CCGCTTTGCA GCAGCAGTTC TGTTCCCAGC
    TTTGCAGAGA GCGTGGCAGT CAGCCCCCTG CTGGATAGTG GCCAAAAGGC AGCAGAAGTG
    GGCACGGTAA TGAGCACGAC TGGCTTGTCT CTGCCCATCT CTGCACCTCT GACATATGCT
    GGGGTGGTTG CAGCATCAGG CATCACAAGT CAACCTGCCG TGCATCTCCC CCAGGGGGTG
    GCCACAACAC CAGTTGTATC CCAGGCAGTA GGGGGAGCTG TGGGCACACC CCTCTCATCT
    CTTCCTCCCA TCCCTGGAGG CATGTCTGTT AGCCAACCAG GATCAAATAT ACCCCCTGTG
    CAACACACGT TGGCCCATGG CCAACCCCAG CCTACCCCTC TCCCCAACCA GCCTCATACG
    CACCTTGTAG AGGGTGACAC CGATAACCTG CCCAAACCTG GCATAGACGA TATAAAAACC
    CTGGAGGAGA AGCTGAGGTC GCTGTTCAGT GAGCATGGCA GTGCCGGGGC CCCTCATGGA
    CTCACCCCAT CAGAAACTCA ATTGGGTTTG GATGGGGCCC CCCTGCCAGG CCCTTCCACA
    GGTAGCACCG CTCAGACCAA GCCTGCAGCA TCCTCTTCTC CACCAACCAG CTTACCTTTA
    GGGCCACCTG GAGTGACGAC AATGACTCAG ATCATGACAC CTGGACAAGC TGCTACACCT
    GTCACCTACG TATCTGCAGC TGGAAGCTCT ATAGCCACGG CCAAAGCTGC CACCCCACCC
    TCAAAGCCGC CTCTCAGTCG GGTACCGGTG ACCACGGCCG GGGCAGAGAC CCCAGCAGGC
    AGTCTCCCTG GTGAACACCT GCCTCCTCCT TCTCTCCCAG GACCTTCACT AACTCAGCCT
    GCACACAGCG GAGCTTCTAC CGGTGTTGCA GCACCCGTCG GCTCAGCATT GCAACCCTCC
    GCTTCAGAAT TGCCAAAAGC CAGCGCTGGA GAGGCAGAGG TGGGCGCGAG TGTCCAGAGG
    ATGGGCAGGT TTCAGGTCTC TTTGGCAGCA GAGAATGTGC TCAAGCAGAG CGAGGAGACC
    AAACTGTCCA TCTCAGACAC TTCCTCCACC ACTTCTACAT CCTCCTCTTC CTCTGCGTCA
    TGCAGCAGCC CAGAGAGCAC CCTGACTAAG CCCCAGGCCA AAACCGCTGC TCCCTTAGAC
    ATAGTGGATG GTGTTGGTCC CCACTTGGTG CATCTACAGA GCCTATCTCC CAAGCAGTTG
    GTGAAAGTGG GGCGCTTCCA GGTTACCACT GCCACCACGG ACAAAGTGGG GCGCTTTTCT
    GTGTCCCGGA CACAAGATGA TATATCTGCT GGGGTGCTGG AGACAGACTC CCAGAGGTCC
    TCCTCCCCAC AGACTGAGAA ACATCTGAAT GGACCCTACT TACAGCAAGA CTCCTCTGCG
    GCTCTCGGCT ATCCGGCCAG GGAGACGCAG GAAGACAGCC TAGGGAGCCC CGTTCTGCAC
    CACGCTTTGG GGTCCAAGCT TAGCCTGCAG AGCCTTAGCA ACTCGTTCAA CTCTTCCTAC
    ATGAGCAGCG ATAATGATTC TGACATAGAA GATGAAGATC TGAAGAAGGA GCTGCGAAGG
    CTACGAGACA AGCATCTCAA GGAGATTCAG GAACTGCAGA GTCGACAGAA GCAGGAGATC
    GAGCTGTTGT ACACGGCTTT GGGAAAAGTG CCCCCTGCTG TCATTATCCC TCCGGCAGCG
    CCCCTTTCTG GCCGGCGCAG GAGGCATACC AAGGGGAAGG GCAGCAAGTC CAGCCGGGGC
    AGCTCCCACA ACAGCCGCAG CCCTCATTTG TCAGGCGACC TGTCCACGCA GAGCGCTCCG
    TCGGCATTAC CCCCCCAGCC GGGCCTTCTG CTCCCCCCAA GCAGTGCGCC CGAACATGCT
    CATCTTCTCA AGCCCTCCCC TTCCAGCGAC ATTCTCTGCT CAGCCTTCAC TAGCGACGCT
    GCCCTCTCTG TGCCCAGCCT GTCCACCCAG GGCCAAGGGA CCAGCAGCAC TAACACAGTC
    GCACCATCAA CTGCCCCGGC CCCCCAGCCA AACGTGCCCC CCGTGTCATC CAGCCGCAAG
    GGAACCTTCA CGGACGATCT ACACAAACTG GTGGACAACT GGGCCCGAGA CGCCATGAAC
    CTGACTGGAA AAAAGGCTTG TAAGGCCTCT GGGCAACCCA GCTATGAGGG ACCCGGGATG
    GCCCGTAAGT TCTCTGCTCC CGGACAGCTG TGTGTCCCCA TGAACCCATC CCTGGGGGGC
    TCCGTCCCTC TCCCAGCAGC TTCGGCGACC TCCCTGGCTC ACTACCCCAA AGGAATGTGC
    CCCCCGCAAC AATACGGAAT CCCCGGGGCG CTGTTCCAAG CTCCATGGAG TGGCACCGGC
    GTGCCCCCCT CCTCTCAGCC CCTAAGCCAG TTCCCCCCGG CGGTGACATC TTCCTTACAG
    AACTTCGGTA TTAGCAGCTT GCAGAAATCT ATCAGCAACC CCCAAGGATC CAACCTGCGG
    ACTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754490.1
    CDS322..6630
    Translation

    Target ORF information:

    RefSeq Version XM_031898630.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCACAAATA TCATCTGGAC AACCACCCAC CACATCCATG
    GGAATCAGTG CTCAGCCCGG TCCCCACACC CAGGGCATGG TGCATCATCA GCCTTCTCTG
    CATCCAGCCT CGCAGTACCA AGTGCAGCAG CCAATGCCTA CTGTGGGTTC CAGTATTGCG
    GCCCCATCAC AGCCGGTGAT TACCCAAACG CCAGCCCCTC AGCCGGGCAC CCAGGTTTTC
    CAGGCTACTC ACCCTCTATT CCCTGGAGAT GTAAGCTTTG TTCCTCCCGT TGCCATCCCC
    TCAGCAATGG TATCTCCCCC TATCCGTGGT GAAAGTTTAG CCCAACCTGG CTTCCATACT
    CAGCCTTATC CTGAAAACAG CAATTTGTAT TCCCATGGAG GGCCCACTCC CCAGCCCGCT
    GTTCTCACTA CCCAGCCGGT GCCTGCAGGA ACCCAGACGC AACCTGCCGT CGAGGGTTCA
    CAGAGTAGCT CCCTGGCAAT TTCCCAGGAT ACCCAGTCAT CTCCTCAAGC AACACAAACT
    GCCTCCTCCA TGGACAGCGC TCATTCAGAC GTGGCATCAG GACTGAGTGA CGGAAACGAG
    GGCAGCACAG GTGGGAGACA CGAAGGCCGA GCCACTAAAC GACACTACCG AAAATCTGTG
    AGAAGCCGCT CCAGGCACGA GAAAACTGCA CGCCCAAAGC TTCGAATCCT TAATGTTTCC
    AACAAAGGGG ATCGTGTTGT AGAGTGTCAG CTGGAGACTC ACAACCGCAA GATGGTCACC
    TTCAAATTCG ACCTGGATGG AGACAATCCA GAGGAGATTG CTACTATAAT GGTACAGAAT
    GTGTTTATTT TGGCTACGGA GAGAGAATCC TTTGTAGAGC AGGTAAAAGA CATAATTGAG
    AAGGCCGATG AAATGTTGAG CGAAGATGTG AGCGTGGAGC CGGAAGGAGA AACGAGGGCA
    GAAAGTTTGC AAATCAAGGA TGATGGGGAT TACCCAGAAT CTCAGAAGAT GAATTCTGAA
    TTTCGAATGC CAGATCCTGT ATATTCCCTA CCACAGACAG CAGGGCTTAT TCCCAGCGTT
    GTCACACAAG TCGTTCATTC TGCCGGAAGA CGATTTATTG TCAGTCCGGT GCCTGAAAGC
    CGTCTAAAAG AACAGTCATT CTTCAGTACT TCATTGCAGG ACCCTCAGCT ACCAGTCTCT
    GTCGCGCATG GTCCAGGGAT GAATTTGTCT CATTCTGCCT CTTCTGTGAG CCTGCAGCAA
    GCCTTTGCAG AACTACGCCA GTCACAGATG AGCGAGGGTC CTAGCACAGC TCCTCCCATA
    TTTAACCAAA CTGGACCCCC CTTTCCACCA TCCATGACTA GTCTGGTGGC AACTCTGCCT
    TCTAATGCAA TGCCTATCCT CCAGGCAGCC CCAGTGATCC CCAGTAGCCT GCCATCTTCT
    GTGCCTCAGC CCCTGGTGGA GGTGGTTGTA CCTCAGGCTG CTGGGATGGC TCCAACTAGC
    AATGTTCCAA GCACCTTGTC CCCTCCCCTA ACTGCAGTAT CCAACCAGCA CGCAGACATG
    ATACCGGGTG CTGCTGTAGC AGCTATCGGA TCACTTCCTG TGACATCACT TCCTCCTTTG
    CCCATTGTGG GTGGAGTTCC TGTAGGCACT GCAACCCTGA GCTCACCCCT AACACCCCTA
    ATCAGTTCTG TCAGTACAAT GGGGGGGACG GTTCCCCCAT CTGTCACATC TCCCCCAGTG
    CCACAGATAG CGGGTAGCGT TGCCGGGAGC CTGCCTCCTG CATCAGCCTC CGCCCCTCTT
    ATTCATGGCA GTGTTGCCCC ATCTCAGGTA CCGCTTTGCA GCAGCAGTTC TGTTCCCAGC
    TTTGCAGAGA GCGTGGCAGT CAGCCCCCTG CTGGATAGTG GCCAAAAGGC AGCAGAAGTG
    GGCACGGTAA TGAGCACGAC TGGCTTGTCT CTGCCCATCT CTGCACCTCT GACATATGCT
    GGGGTGGTTG CAGCATCAGG CATCACAAGT CAACCTGCCG TGCATCTCCC CCAGGGGGTG
    GCCACAACAC CAGTTGTATC CCAGGCAGTA GGGGGAGCTG TGGGCACACC CCTCTCATCT
    CTTCCTCCCA TCCCTGGAGG CATGTCTGTT AGCCAACCAG GATCAAATAT ACCCCCTGTG
    CAACACACGT TGGCCCATGG CCAACCCCAG CCTACCCCTC TCCCCAACCA GCCTCATACG
    CACCTTGTAG AGGGTGACAC CGATAACCTG CCCAAACCTG GCATAGACGA TATAAAAACC
    CTGGAGGAGA AGCTGAGGTC GCTGTTCAGT GAGCATGGCA GTGCCGGGGC CCCTCATGGA
    CTCACCCCAT CAGAAACTCA ATTGGGTTTG GATGGGGCCC CCCTGCCAGG CCCTTCCACA
    GGTAGCACCG CTCAGACCAA GCCTGCAGCA TCCTCTTCTC CACCAACCAG CTTACCTTTA
    GGGCCACCTG GAGTGACGAC AATGACTCAG ATCATGACAC CTGGACAAGC TGCTACACCT
    GTCACCTACG TATCTGCAGC TGGAAGCTCT ATAGCCACGG CCAAAGCTGC CACCCCACCC
    TCAAAGCCGC CTCTCAGTCG GGTACCGGTG ACCACGGCCG GGGCAGAGAC CCCAGCAGGC
    AGTCTCCCTG GTGAACACCT GCCTCCTCCT TCTCTCCCAG GACCTTCACT AACTCAGCCT
    GCACACAGCG GAGCTTCTAC CGGTGTTGCA GCACCCGTCG GCTCAGCATT GCAACCCTCC
    GCTTCAGAAT TGCCAAAAGC CAGCGCTGGA GAGGCAGAGG TGGGCGCGAG TGTCCAGAGG
    ATGGGCAGGT TTCAGGTCTC TTTGGCAGCA GAGAATGTGC TCAAGCAGAG CGAGGAGACC
    AAACTGTCCA TCTCAGACAC TTCCTCCACC ACTTCTACAT CCTCCTCTTC CTCTGCGTCA
    TGCAGCAGCC CAGAGAGCAC CCTGACTAAG CCCCAGGCCA AAACCGCTGC TCCCTTAGAC
    ATAGTGGATG GTGTTGGTCC CCACTTGGTG CATCTACAGA GCCTATCTCC CAAGCAGTTG
    GTGAAAGTGG GGCGCTTCCA GGTTACCACT GCCACCACGG ACAAAGTGGG GCGCTTTTCT
    GTGTCCCGGA CACAAGATGA TATATCTGCT GGGGTGCTGG AGACAGACTC CCAGAGGTCC
    TCCTCCCCAC AGACTGAGAA ACATCTGAAT GGACCCTACT TACAGCAAGA CTCCTCTGCG
    GCTCTCGGCT ATCCGGCCAG GGAGACGCAG GAAGACAGCC TAGGGAGCCC CGTTCTGCAC
    CACGCTTTGG GGTCCAAGCT TAGCCTGCAG AGCCTTAGCA ACTCGTTCAA CTCTTCCTAC
    ATGAGCAGCG ATAATGATTC TGACATAGAA GATGAAGATC TGAAGAAGGA GCTGCGAAGG
    CTACGAGACA AGCATCTCAA GGAGATTCAG GAACTGCAGA GTCGACAGAA GCAGGAGATC
    GAGCTGTTGT ACACGGCTTT GGGAAAAGTG CCCCCTGCTG TCATTATCCC TCCGGCAGCG
    CCCCTTTCTG GCCGGCGCAG GAGGCATACC AAGGGGAAGG GCAGCAAGTC CAGCCGGGGC
    AGCTCCCACA ACAGCCGCAG CCCTCATTTG TCAGGCGACC TGTCCACGCA GAGCGCTCCG
    TCGGCATTAC CCCCCCAGCC GGGCCTTCTG CTCCCCCCAA GCAGTGCGCC CGAACATGCT
    CATCTTCTCA AGCCCTCCCC TTCCAGCGAC ATTCTCTGCT CAGCCTTCAC TAGCGACGCT
    GCCCTCTCTG TGCCCAGCCT GTCCACCCAG GGCCAAGGGA CCAGCAGCAC TAACACAGTC
    GCACCATCAA CTGCCCCGGC CCCCCAGCCA AACGTGCCCC CCGTGTCATC CAGCCGCAAG
    GGAACCTTCA CGGACGATCT ACACAAACTG GTGGACAACT GGGCCCGAGA CGCCATGAAC
    CTGACTGGAA AAAAGGCTTG TAAGGCCTCT GGGCAACCCA GCTATGAGGG ACCCGGGATG
    GCCCGTAAGT TCTCTGCTCC CGGACAGCTG TGTGTCCCCA TGAACCCATC CCTGGGGGGC
    TCCGTCCCTC TCCCAGCAGC TTCGGCGACC TCCCTGGCTC ACTACCCCAA AGGAATGTGC
    CCCCCGCAAC AATACGGAAT CCCCGGGGCG CTGTTCCAAG CTCCATGGAG TGGCACCGGC
    GTGCCCCCCT CCTCTCAGCC CCTAAGCCAG TTCCCCCCGG CGGTGACATC TTCCTTACAG
    AACTTCGGTA TTAGCAGCTT GCAGAAATCT ATCAGCAACC CCCAAGGATC CAACCTGCGG
    ACTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54267
    Clone ID Related Accession (Same CDS sequence) XM_031898626.1
    Accession Version XM_031898626.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGGTTTTCC AGGCTACTCA CCCTCTATTC
    CCTGGAGATG TAAGCTTTGT TCCTCCCGTT GCCATCCCCT CAGCAATGGT ATCTCCCCCT
    ATCCGTGGTG AAAGTTTAGC CCAACCTGGC TTCCATACTC AGCCTTATCC TGAAAACAGC
    AATTTGTATT CCCATGGAGG GCCCACTCCC CAGCCCGCTG TTCTCACTAC CCAGCCGGTG
    CCTGCAGGAA CCCAGACGCA ACCTGCCGTC GAGGGTTCAC AGAGTAGCTC CCTGGCAATT
    TCCCAGGATA CCCAGTCATC TCCTCAAGCA ACACAAACTG CCTCCTCCAT GGACAGCGCT
    CATTCAGACG TGGCATCAGG ACTGAGTGAC GGAAACGAGG GCAGCACAGG TGGGAGACAC
    GAAGGCCGAG CCACTAAACG ACACTACCGA AAATCTGTGA GAAGCCGCTC CAGGCACGAG
    AAAACTGCAC GCCCAAAGCT TCGAATCCTT AATGTTTCCA ACAAAGGGGA TCGTGTTGTA
    GAGTGTCAGC TGGAGACTCA CAACCGCAAG ATGGTCACCT TCAAATTCGA CCTGGATGGA
    GACAATCCAG AGGAGATTGC TACTATAATG GTACAGAATG TGTTTATTTT GGCTACGGAG
    AGAGAATCCT TTGTAGAGCA GGTAAAAGAC ATAATTGAGA AGGCCGATGA AATGTTGAGC
    GAAGATGTGA GCGTGGAGCC GGAAGGAGAA ACGAGGGCAG AAAGTTTGCA AATCAAGGAT
    GATGGGGATT ACCCAGAATC TCAGAAGATG AATTCTGAAT TTCGAATGCC AGATCCTGTA
    TATTCCCTAC CACAGACAGC AGGGCTTATT CCCAGCGTTG TCACACAAGT CGTTCATTCT
    GCCGGAAGAC GATTTATTGT CAGTCCGGTG CCTGAAAGCC GTCTAAAAGA ACAGTCATTC
    TTCAGTACTT CATTGCAGGA CCCTCAGCTA CCAGTCTCTG TCGCGCATGG TCCAGGGATG
    AATTTGTCTC ATTCTGCCTC TTCTGTGAGC CTGCAGCAAG CCTTTGCAGA ACTACGCCAG
    TCACAGATGA GCGAGGGTCC TAGCACAGCT CCTCCCATAT TTAACCAAAC TGGACCCCCC
    TTTCCACCAT CCATGACTAG TCTGGTGGCA ACTCTGCCTT CTAATGCAAT GCCTATCCTC
    CAGGCAGCCC CAGTGATCCC CAGTAGCCTG CCATCTTCTG TGCCTCAGCC CCTGGTGGAG
    GTGGTTGTAC CTCAGGCTGC TGGGATGGCT CCAACTAGCA ATGTTCCAAG CACCTTGTCC
    CCTCCCCTAA CTGCAGTATC CAACCAGCAC GCAGACATGA TACCGGGTGC TGCTGTAGCA
    GCTATCGGAT CACTTCCTGT GACATCACTT CCTCCTTTGC CCATTGTGGG TGGAGTTCCT
    GTAGGCACTG CAACCCTGAG CTCACCCCTA ACACCCCTAA TCAGTTCTGT CAGTACAATG
    GGGGGGACGG TTCCCCCATC TGTCACATCT CCCCCAGTGC CACAGATAGC GGGTAGCGTT
    GCCGGGAGCC TGCCTCCTGC ATCAGCCTCC GCCCCTCTTA TTCATGGCAG TGTTGCCCCA
    TCTCAGGTAC CGCTTTGCAG CAGCAGTTCT GTTCCCAGCT TTGCAGAGAG CGTGGCAGTC
    AGCCCCCTGC TGGATAGTGG CCAAAAGGCA GCAGAAGTGG GCACGGTAAT GAGCACGACT
    GGCTTGTCTC TGCCCATCTC TGCACCTCTG ACATATGCTG GGGTGGTTGC AGCATCAGGC
    ATCACAAGTC AACCTGCCGT GCATCTCCCC CAGGGGGTGG CCACAACACC AGTTGTATCC
    CAGGCAGTAG GGGGAGCTGT GGGCACACCC CTCTCATCTC TTCCTCCCAT CCCTGGAGGC
    ATGTCTGTTA GCCAACCAGG ATCAAATATA CCCCCTGTGC AACACACGTT GGCCCATGGC
    CAACCCCAGC CTACCCCTCT CCCCAACCAG CCTCATACGC ACCTTGTAGA GGGTGACACC
    GATAACCTGC CCAAACCTGG CATAGACGAT ATAAAAACCC TGGAGGAGAA GCTGAGGTCG
    CTGTTCAGTG AGCATGGCAG TGCCGGGGCC CCTCATGGAC TCACCCCATC AGAAACTCAA
    TTGGGTTTGG ATGGGGCCCC CCTGCCAGGC CCTTCCACAG GTAGCACCGC TCAGACCAAG
    CCTGCAGCAT CCTCTTCTCC ACCAACCAGC TTACCTTTAG GGCCACCTGG AGTGACGACA
    ATGACTCAGA TCATGACACC TGGACAAGCT GCTACACCTG TCACCTACGT ATCTGCAGCT
    GGAAGCTCTA TAGCCACGGC CAAAGCTGCC ACCCCACCCT CAAAGCCGCC TCTCAGTCGG
    GTACCGGTGA CCACGGCCGG GGCAGAGACC CCAGCAGGCA GTCTCCCTGG TGAACACCTG
    CCTCCTCCTT CTCTCCCAGG ACCTTCACTA ACTCAGTCCC AGCAGCCTCT AGAAGACCTG
    GATTTCCAGC TGAGAAGGAC GTTGAGCCCT GACACTGTCC CTGTGCCTTC TGCACTAATA
    TTTCCTGCAC ACAGCGGAGC TTCTACCGGT GTTGCAGCAC CCGTCGGCTC AGCATTGCAA
    CCCTCCGCTT CAGAATTGCC AAAAGCCAGC GCTGGAGAGG CAGAGGTGGG CGCGAGTGTC
    CAGAGGATGG GCAGGTTTCA GGTCTCTTTG GCAGCAGAGA ATGTGCTCAA GCAGAGCGAG
    GAGACCAAAC TGTCCATCTC AGACACTTCC TCCACCACTT CTACATCCTC CTCTTCCTCT
    GCGTCATGCA GCAGCCCAGA GAGCACCCTG ACTAAGCCCC AGGCCAAAAC CGCTGCTCCC
    TTAGACATAG TGGATGGTGT TGGTCCCCAC TTGGTGCATC TACAGAGCCT ATCTCCCAAG
    CAGTTGGTGA AAGTGGGGCG CTTCCAGGTT ACCACTGCCA CCACGGACAA AGTGGGGCGC
    TTTTCTGTGT CCCGGACACA AGATGATATA TCTGCTGGGG TGCTGGAGAC AGACTCCCAG
    AGGTCCTCCT CCCCACAGAC TGAGAAACAT CTGAATGGAC CCTACTTACA GCAAGACTCC
    TCTGCGGCTC TCGGCTATCC GGCCAGGGAG ACGCAGGAAG ACAGCCTAGG GAGCCCCGTT
    CTGCACCACG CTTTGGGGTC CAAGCTTAGC CTGCAGAGCC TTAGCAACTC GTTCAACTCT
    TCCTACATGA GCAGCGATAA TGATTCTGAC ATAGAAGATG AAGATCTGAA GAAGGAGCTG
    CGAAGGCTAC GAGACAAGCA TCTCAAGGAG ATTCAGGAAC TGCAGAGTCG ACAGAAGCAG
    GAGATCGAGC TGTTGTACAC GGCTTTGGGA AAAGTGCCCC CTGCTGTCAT TATCCCTCCG
    GCAGCGCCCC TTTCTGGCCG GCGCAGGAGG CATACCAAGG GGAAGGGCAG CAAGTCCAGC
    CGGGGCAGCT CCCACAACAG CCGCAGCCCT CATTTGTCAG GCGACCTGTC CACGCAGAGC
    GCTCCGTCGG CATTACCCCC CCAGCCGGGC CTTCTGCTCC CCCCAAGCAG TGCGCCCGAA
    CATGCTCATC TTCTCAAGCC CTCCCCTTCC AGCGACATTC TCTGCTCAGC CTTCACTAGC
    GACGCTGCCC TCTCTGTGCC CAGCCTGTCC ACCCAGGGCC AAGGCTGGGC CAAGTTTAAC
    TGTGAGTCAG AGCGGGTATC CTTCAAAATG GCTGGCAGGA GGACGCGATT TCTGAGTACG
    CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGTC CTTGCAACCA GCTCTGTAGG
    ACCAGCAGCA CTAACACAGT CGCACCATCA ACTGCCCCGG CCCCCCAGCC AAACGTGCCC
    CCCGTGTCAT CCAGCCGCAA GGGAACCTTC ACGGACGATC TACACAAACT GGTGGACAAC
    TGGGCCCGAG ACGCCATGAA CCTGACTGGA AAAAAGGCTT GTAAGGCCTC TGGGCAACCC
    AGCTATGAGG GACCCGGGAT GGCCCGTAAG TTCTCTGCTC CCGGACAGCT GTGTGTCCCC
    ATGAACCCAT CCCTGGGGGG CTCCGTCCCT CTCCCAGCAG CTTCGGCGAC CTCCCTGGCT
    CACTACCCCA AAGGAATGTG CCCCCCGCAA CAATACGGAA TCCCCGGGGC GCTGTTCCAA
    GCTCCATGGA GTGGCACCGG CGTGCCCCCC TCCTCTCAGC CCCTAAGCCA GTTCCCCCCG
    GCGGTGACAT CTTCCTTACA GAACTTCGGT ATTAGCAGCT TGCAGAAATC TATCAGCAAC
    CCCCAAGGAT CCAACCTGCG GACTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754486.1
    CDS327..8216
    Translation

    Target ORF information:

    RefSeq Version XM_031898626.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGGTTTTCC AGGCTACTCA CCCTCTATTC
    CCTGGAGATG TAAGCTTTGT TCCTCCCGTT GCCATCCCCT CAGCAATGGT ATCTCCCCCT
    ATCCGTGGTG AAAGTTTAGC CCAACCTGGC TTCCATACTC AGCCTTATCC TGAAAACAGC
    AATTTGTATT CCCATGGAGG GCCCACTCCC CAGCCCGCTG TTCTCACTAC CCAGCCGGTG
    CCTGCAGGAA CCCAGACGCA ACCTGCCGTC GAGGGTTCAC AGAGTAGCTC CCTGGCAATT
    TCCCAGGATA CCCAGTCATC TCCTCAAGCA ACACAAACTG CCTCCTCCAT GGACAGCGCT
    CATTCAGACG TGGCATCAGG ACTGAGTGAC GGAAACGAGG GCAGCACAGG TGGGAGACAC
    GAAGGCCGAG CCACTAAACG ACACTACCGA AAATCTGTGA GAAGCCGCTC CAGGCACGAG
    AAAACTGCAC GCCCAAAGCT TCGAATCCTT AATGTTTCCA ACAAAGGGGA TCGTGTTGTA
    GAGTGTCAGC TGGAGACTCA CAACCGCAAG ATGGTCACCT TCAAATTCGA CCTGGATGGA
    GACAATCCAG AGGAGATTGC TACTATAATG GTACAGAATG TGTTTATTTT GGCTACGGAG
    AGAGAATCCT TTGTAGAGCA GGTAAAAGAC ATAATTGAGA AGGCCGATGA AATGTTGAGC
    GAAGATGTGA GCGTGGAGCC GGAAGGAGAA ACGAGGGCAG AAAGTTTGCA AATCAAGGAT
    GATGGGGATT ACCCAGAATC TCAGAAGATG AATTCTGAAT TTCGAATGCC AGATCCTGTA
    TATTCCCTAC CACAGACAGC AGGGCTTATT CCCAGCGTTG TCACACAAGT CGTTCATTCT
    GCCGGAAGAC GATTTATTGT CAGTCCGGTG CCTGAAAGCC GTCTAAAAGA ACAGTCATTC
    TTCAGTACTT CATTGCAGGA CCCTCAGCTA CCAGTCTCTG TCGCGCATGG TCCAGGGATG
    AATTTGTCTC ATTCTGCCTC TTCTGTGAGC CTGCAGCAAG CCTTTGCAGA ACTACGCCAG
    TCACAGATGA GCGAGGGTCC TAGCACAGCT CCTCCCATAT TTAACCAAAC TGGACCCCCC
    TTTCCACCAT CCATGACTAG TCTGGTGGCA ACTCTGCCTT CTAATGCAAT GCCTATCCTC
    CAGGCAGCCC CAGTGATCCC CAGTAGCCTG CCATCTTCTG TGCCTCAGCC CCTGGTGGAG
    GTGGTTGTAC CTCAGGCTGC TGGGATGGCT CCAACTAGCA ATGTTCCAAG CACCTTGTCC
    CCTCCCCTAA CTGCAGTATC CAACCAGCAC GCAGACATGA TACCGGGTGC TGCTGTAGCA
    GCTATCGGAT CACTTCCTGT GACATCACTT CCTCCTTTGC CCATTGTGGG TGGAGTTCCT
    GTAGGCACTG CAACCCTGAG CTCACCCCTA ACACCCCTAA TCAGTTCTGT CAGTACAATG
    GGGGGGACGG TTCCCCCATC TGTCACATCT CCCCCAGTGC CACAGATAGC GGGTAGCGTT
    GCCGGGAGCC TGCCTCCTGC ATCAGCCTCC GCCCCTCTTA TTCATGGCAG TGTTGCCCCA
    TCTCAGGTAC CGCTTTGCAG CAGCAGTTCT GTTCCCAGCT TTGCAGAGAG CGTGGCAGTC
    AGCCCCCTGC TGGATAGTGG CCAAAAGGCA GCAGAAGTGG GCACGGTAAT GAGCACGACT
    GGCTTGTCTC TGCCCATCTC TGCACCTCTG ACATATGCTG GGGTGGTTGC AGCATCAGGC
    ATCACAAGTC AACCTGCCGT GCATCTCCCC CAGGGGGTGG CCACAACACC AGTTGTATCC
    CAGGCAGTAG GGGGAGCTGT GGGCACACCC CTCTCATCTC TTCCTCCCAT CCCTGGAGGC
    ATGTCTGTTA GCCAACCAGG ATCAAATATA CCCCCTGTGC AACACACGTT GGCCCATGGC
    CAACCCCAGC CTACCCCTCT CCCCAACCAG CCTCATACGC ACCTTGTAGA GGGTGACACC
    GATAACCTGC CCAAACCTGG CATAGACGAT ATAAAAACCC TGGAGGAGAA GCTGAGGTCG
    CTGTTCAGTG AGCATGGCAG TGCCGGGGCC CCTCATGGAC TCACCCCATC AGAAACTCAA
    TTGGGTTTGG ATGGGGCCCC CCTGCCAGGC CCTTCCACAG GTAGCACCGC TCAGACCAAG
    CCTGCAGCAT CCTCTTCTCC ACCAACCAGC TTACCTTTAG GGCCACCTGG AGTGACGACA
    ATGACTCAGA TCATGACACC TGGACAAGCT GCTACACCTG TCACCTACGT ATCTGCAGCT
    GGAAGCTCTA TAGCCACGGC CAAAGCTGCC ACCCCACCCT CAAAGCCGCC TCTCAGTCGG
    GTACCGGTGA CCACGGCCGG GGCAGAGACC CCAGCAGGCA GTCTCCCTGG TGAACACCTG
    CCTCCTCCTT CTCTCCCAGG ACCTTCACTA ACTCAGTCCC AGCAGCCTCT AGAAGACCTG
    GATTTCCAGC TGAGAAGGAC GTTGAGCCCT GACACTGTCC CTGTGCCTTC TGCACTAATA
    TTTCCTGCAC ACAGCGGAGC TTCTACCGGT GTTGCAGCAC CCGTCGGCTC AGCATTGCAA
    CCCTCCGCTT CAGAATTGCC AAAAGCCAGC GCTGGAGAGG CAGAGGTGGG CGCGAGTGTC
    CAGAGGATGG GCAGGTTTCA GGTCTCTTTG GCAGCAGAGA ATGTGCTCAA GCAGAGCGAG
    GAGACCAAAC TGTCCATCTC AGACACTTCC TCCACCACTT CTACATCCTC CTCTTCCTCT
    GCGTCATGCA GCAGCCCAGA GAGCACCCTG ACTAAGCCCC AGGCCAAAAC CGCTGCTCCC
    TTAGACATAG TGGATGGTGT TGGTCCCCAC TTGGTGCATC TACAGAGCCT ATCTCCCAAG
    CAGTTGGTGA AAGTGGGGCG CTTCCAGGTT ACCACTGCCA CCACGGACAA AGTGGGGCGC
    TTTTCTGTGT CCCGGACACA AGATGATATA TCTGCTGGGG TGCTGGAGAC AGACTCCCAG
    AGGTCCTCCT CCCCACAGAC TGAGAAACAT CTGAATGGAC CCTACTTACA GCAAGACTCC
    TCTGCGGCTC TCGGCTATCC GGCCAGGGAG ACGCAGGAAG ACAGCCTAGG GAGCCCCGTT
    CTGCACCACG CTTTGGGGTC CAAGCTTAGC CTGCAGAGCC TTAGCAACTC GTTCAACTCT
    TCCTACATGA GCAGCGATAA TGATTCTGAC ATAGAAGATG AAGATCTGAA GAAGGAGCTG
    CGAAGGCTAC GAGACAAGCA TCTCAAGGAG ATTCAGGAAC TGCAGAGTCG ACAGAAGCAG
    GAGATCGAGC TGTTGTACAC GGCTTTGGGA AAAGTGCCCC CTGCTGTCAT TATCCCTCCG
    GCAGCGCCCC TTTCTGGCCG GCGCAGGAGG CATACCAAGG GGAAGGGCAG CAAGTCCAGC
    CGGGGCAGCT CCCACAACAG CCGCAGCCCT CATTTGTCAG GCGACCTGTC CACGCAGAGC
    GCTCCGTCGG CATTACCCCC CCAGCCGGGC CTTCTGCTCC CCCCAAGCAG TGCGCCCGAA
    CATGCTCATC TTCTCAAGCC CTCCCCTTCC AGCGACATTC TCTGCTCAGC CTTCACTAGC
    GACGCTGCCC TCTCTGTGCC CAGCCTGTCC ACCCAGGGCC AAGGCTGGGC CAAGTTTAAC
    TGTGAGTCAG AGCGGGTATC CTTCAAAATG GCTGGCAGGA GGACGCGATT TCTGAGTACG
    CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGTC CTTGCAACCA GCTCTGTAGG
    ACCAGCAGCA CTAACACAGT CGCACCATCA ACTGCCCCGG CCCCCCAGCC AAACGTGCCC
    CCCGTGTCAT CCAGCCGCAA GGGAACCTTC ACGGACGATC TACACAAACT GGTGGACAAC
    TGGGCCCGAG ACGCCATGAA CCTGACTGGA AAAAAGGCTT GTAAGGCCTC TGGGCAACCC
    AGCTATGAGG GACCCGGGAT GGCCCGTAAG TTCTCTGCTC CCGGACAGCT GTGTGTCCCC
    ATGAACCCAT CCCTGGGGGG CTCCGTCCCT CTCCCAGCAG CTTCGGCGAC CTCCCTGGCT
    CACTACCCCA AAGGAATGTG CCCCCCGCAA CAATACGGAA TCCCCGGGGC GCTGTTCCAA
    GCTCCATGGA GTGGCACCGG CGTGCCCCCC TCCTCTCAGC CCCTAAGCCA GTTCCCCCCG
    GCGGTGACAT CTTCCTTACA GAACTTCGGT ATTAGCAGCT TGCAGAAATC TATCAGCAAC
    CCCCAAGGAT CCAACCTGCG GACTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54268
    Clone ID Related Accession (Same CDS sequence) XM_031898627.1
    Accession Version XM_031898627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCCCCAG AGATGTATGA GGAGAAATAC GATGAGTCCG TTGACGTGTA TGCGTTTGGG 
    ATGTGCATGC TTGAGATGGC CACTTCTGAA TACCCTTACT CCGAATGCCA AAACGCTGCA
    CAGATCTACC GGCGAGTCAC CAGTGGCGTG AAGCCTGCCA GCTTCGATAA AGTCGCAATT
    CCAGAGGTGA AAGAAATCAT CGAAGGGTGC ATTCGCCAAA ACAAGGACGA AAGGTATGCC
    ATTAAGGACC TGCTGAACCA TGCCTTTTTC CAAGAGGAAA CTGGGGTCCG CGTGGAACTG
    GCAGAGGAAG ATGACGGGGA AAAGATTGCT ATCAAGCTTT GGCTGCGTAT TGAGGATATC
    AAAAAACTCA AGGGCAAATA CAAGGACAAT GAGGCAATTG AGTTCTCCTT TGACCTGGAG
    CGAGATGTTC CGGATGATGT GGCACAGGAA ATGGTGGAAT CTGGCTACGT CTGTGAAGGA
    GACCACAAGA CTATGGCAAA GGCAATCAAA GATAGGGTGT CCCTGATCAA ACGCAAGCGG
    GAACAGAGGC AGCAAGTGCG GGAAGAGCAG GAGAAGAGGA AACAGGAGGA GATTAGCCAG
    CAGCAGCAGC AGCAACAAGC AGAGCTTCAG CCTCAGAGTC CTCCTCAAGG CACCGTCAAA
    CCCTCCACTG GCACCGTTAC CTCTGGCACA CAAGTAGAAC CCGAGGAGCC GGAAGCTGAT
    CAGCATCTAC AGTACCAGCA GCCAAGTGTA TCCGTCACAT CATTAGCCGA TGGGACAATT
    GACAGTGGTC AGGGATCGTC CATGTATTCT GAATCTTATG CGGGAAACCA GCCGACAGTC
    TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC ACTCCCAGCA TGGTGCCTGG CTTCAGCACT
    CCAAATATCC AACCCCCTGC CCAGCATGGC GGATACCCAG CTCCTGTAGG GCCTTGTCGA
    GGTCGTAGCA TGTCAGTATG TGTTCCCAAC CTTTCCTCTG CTCCCCCACT CCTTTCCGTT
    GTGCCCCCCA GTGCTCCAGG CTCCCCTTCC CCATTGCAGT CTGCCCCTGC CTCCCCACTC
    TGGACATCTC CCGAAGATTC CTTTGCTGAA AAGCTTTCCA AGGCTCTAGA GAGTGTTCTG
    CCCATGCACT CCTCCAGCCC TCGCAAGCAC CAACGCCGCT GCAGCCTGCC TACCCTCTAT
    ACCAGCCCTC CATCGTCCAT GGTCCATTCA GCACTCCCAG ACTCTCCGCT TTTTTTCCCA
    ACCATTCCCA ACCAGCGCCC CATGTCTTTC TCTCCGCCGC CTACTGGCTT GTCCCCAAAG
    GTGCCTGCAA CCCAGCGACG CAAGAGCACC TCTTTCTTGG AGTCCCAAGG CCGCCAGGGG
    CTATCTCAGA TTAGGACACT CGGGCCAGGT GAGGAGACCC AGTGCTGGAG GGCAGACTAC
    ATGGCAGCTC TAGGTAAAGC ATCTGTCCGA TCCGAGGATG CTCACAAGCA TCAAAACTAT
    GAGGGATTGG CGTTCCATGA CGCAAGACAT GTTCCGACAG TTCCAAATGA AACTCTCTTA
    AAGCCTGGGG AAGCATTGTA TCTAGCCAGT CTGCCCTCAC AGGAGAGCTA CAGCAGCATG
    GCTGCCCCTG AGCATATGGA GGGCCACAAG CTGACCCAGC TTGGCTCGTT TTACGATTAT
    CACACTGGGC AAACCTACAT TGCGCGGCCC CTGAAGAGCC TAAGACTGGA ATCTGGGTTA
    AACCAGCCGT CGGCTTTGGG TAGCATCTCC AACCCAATCT CCTCCGACCC ACACGCCATG
    TTTCATCAGA TGTTTGTGCC CCAGTCTGCT CCTCCAGTGC TGGCTCAAAG CAGCGAAGGC
    CACCCAGGGT GTGTGTTTGA GTTCCACGTG CACCCATCTG GGTCTGTTCC CGGCGAAGCA
    ACCGTTTACT TGCCACACCG AGTTTACCGT CCTCGACGGG GCTCTGTAGA CTTTAACCCA
    GAAGAACAGC CGCCGGGTTC TACGCTGCAG AGCCGCCTCC AAACTGTGAC TGAAGAGCAG
    TGCCACCACA ATGGCCCAGA ACCACTCCCA CCCTTTATAG GATTCATCAC ATGCAACCGT
    ATGGAGAGCA GCCCCGACTA TTCCAGTGAT TCTTCCCAAA GGAATTCATC CGATCCCGAT
    TATCTCTCTC CTCCTCTGGC CTTCAGCTCC AATATCTCTA TGGCTTCCAA GCAGAAAACG
    TTGCAGGATC CACAGATTTT CTTTTGTGTC CCACAGTCCG GAACGACGGC TTATGCTGGA
    CCATCAGCTT TGTACAGCCA GCAGCCACAA ATATCATCTG GACAACCACC CACCACATCC
    ATGGGAATCA GTGCTCAGCC CGGTCCCCAC ACCCAGGGCA TGGTGCATCA TCAGCCTTCT
    CTGCATCCAG CCTCGCAGTA CCAAGTGCAG CAGCCAATGC CTACTGTGGG TTCCAGTATT
    GCGGCCCCAT CACAGCCGGT GATTACCCAA ACGCCAGCCC CTCAGCCGGG CACCCAGCTC
    CCTGCCTCCC AGCCTTTGCC TGCTGTTCAA GGAGAGCCTC AGATTCCTGC TCAGACAGCT
    CCTGCTCCCC AGCCCGTTCC TACAGTGCCG GCCTCTCTGC CACCTCATTT TCTTCCCTTG
    GCACAATCTT TGGTTGCAGT CCCTCTCATT CCTCCATTCG CTGCCCCACA GTTGCCTTTA
    ACGGTACCAC CCCCGGTCAT ACAGACAGGG TTCCCTTCCC TACCCATGAC TGTAACCCCA
    AGTATTGGCC AAACTGTACC AGGAATACCC CCATCCTTAG GAGCGCCAGT CCTGCCCCAT
    GGGCAGGCAG TGTTAGTTCC CACTCACATC ACTAATGTTG CTCTCCAGCC TTTGGCACCA
    ATCCCCACAC CTCCTCTGTG CCCTGTAACT CAGCCCATTG GATTACCGGC CACCCTGGGA
    AAAGCTGCTG AGCCCTTGCT TCCTCCGGGA GATACTTCTT ACCAGGTTTT CCAGGCTACT
    CACCCTCTAT TCCCTGGAGA TGTAAGCTTT GTTCCTCCCG TTGCCATCCC CTCAGCAATG
    GTATCTCCCC CTATCCGTGG TGAAAGTTTA GCCCAACCTG GCTTCCATAC TCAGCCTTAT
    CCTGAAAACA GCAATTTGTA TTCCCATGGA GGGCCCACTC CCCAGCCCGC TGTTCTCACT
    ACCCAGCCGG TGCCTGCAGG AACCCAGACG CAACCTGCCG TCGAGGGTTC ACAGAGTAGC
    TCCCTGGCAA TTTCCCAGGA TACCCAGTCA TCTCCTCAAG CAACACAAAC TGCCTCCTCC
    ATGGACAGCG CTCATTCAGA CGTGGCATCA GGACTGAGTG ACGGAAACGA GGGCAGCACA
    GGTGGGAGAC ACGAAGGCCG AGCCACTAAA CGACACTACC GAAAATCTGT GAGAAGCCGC
    TCCAGGCACG AGAAAACTGC ACGCCCAAAG CTTCGAATCC TTAATGTTTC CAACAAAGGG
    GATCGTGTTG TAGAGTGTCA GCTGGAGACT CACAACCGCA AGATGGTCAC CTTCAAATTC
    GACCTGGATG GAGACAATCC AGAGGAGATT GCTACTATAA TGGTACAGAA TGTGTTTATT
    TTGGCTACGG AGAGAGAATC CTTTGTAGAG CAGGTAAAAG ACATAATTGA GAAGGCCGAT
    GAAATGTTGA GCGAAGATGT GAGCGTGGAG CCGGAAGGAG AAACGAGGGC AGAAAGTTTG
    CAAATCAAGG ATGATGGGGA TTACCCAGAA TCTCAGAAGA TGAATTCTGA ATTTCGAATG
    CCAGATCCTG TATATTCCCT ACCACAGACA GCAGGGCTTA TTCCCAGCGT TGTCACACAA
    GTCGTTCATT CTGCCGGAAG ACGATTTATT GTCAGTCCGG TGCCTGAAAG CCGTCTAAAA
    GAACAGTCAT TCTTCAGTAC TTCATTGCAG GACCCTCAGC TACCAGTCTC TGTCGCGCAT
    GGTCCAGGGA TGAATTTGTC TCATTCTGCC TCTTCTGTGA GCCTGCAGCA AGCCTTTGCA
    GAACTACGCC AGTCACAGAT GAGCGAGGGT CCTAGCACAG CTCCTCCCAT ATTTAACCAA
    ACTGGACCCC CCTTTCCACC ATCCATGACT AGTCTGGTGG CAACTCTGCC TTCTAATGCA
    ATGCCTATCC TCCAGGCAGC CCCAGTGATC CCCAGTAGCC TGCCATCTTC TGTGCCTCAG
    CCCCTGGTGG AGGTGGTTGT ACCTCAGGCT GCTGGGATGG CTCCAACTAG CAATGTTCCA
    AGCACCTTGT CCCCTCCCCT AACTGCAGTA TCCAACCAGC ACGCAGACAT GATACCGGGT
    GCTGCTGTAG CAGCTATCGG ATCACTTCCT GTGACATCAC TTCCTCCTTT GCCCATTGTG
    GGTGGAGTTC CTGTAGGCAC TGCAACCCTG AGCTCACCCC TAACACCCCT AATCAGTTCT
    GTCAGTACAA TGGGGGGGAC GGTTCCCCCA TCTGTCACAT CTCCCCCAGT GCCACAGATA
    GCGGGTAGCG TTGCCGGGAG CCTGCCTCCT GCATCAGCCT CCGCCCCTCT TATTCATGGC
    AGTGTTGCCC CATCTCAGGT ACCGCTTTGC AGCAGCAGTT CTGTTCCCAG CTTTGCAGAG
    AGCGTGGCAG TCAGCCCCCT GCTGGATAGT GGCCAAAAGG CAGCAGAAGT GGGCACGGTA
    ATGAGCACGA CTGGCTTGTC TCTGCCCATC TCTGCACCTC TGACATATGC TGGGGTGGTT
    GCAGCATCAG GCATCACAAG TCAACCTGCC GTGCATCTCC CCCAGGGGGT GGCCACAACA
    CCAGTTGTAT CCCAGGCAGT AGGGGGAGCT GTGGGCACAC CCCTCTCATC TCTTCCTCCC
    ATCCCTGGAG GCATGTCTGT TAGCCAACCA GGATCAAATA TACCCCCTGT GCAACACACG
    TTGGCCCATG GCCAACCCCA GCCTACCCCT CTCCCCAACC AGCCTCATAC GCACCTTGTA
    GAGGGTGACA CCGATAACCT GCCCAAACCT GGCATAGACG ATATAAAAAC CCTGGAGGAG
    AAGCTGAGGT CGCTGTTCAG TGAGCATGGC AGTGCCGGGG CCCCTCATGG ACTCACCCCA
    TCAGAAACTC AATTGGGTTT GGATGGGGCC CCCCTGCCAG GCCCTTCCAC AGGTAGCACC
    GCTCAGACCA AGCCTGCAGC ATCCTCTTCT CCACCAACCA GCTTACCTTT AGGGCCACCT
    GGAGTGACGA CAATGACTCA GATCATGACA CCTGGACAAG CTGCTACACC TGTCACCTAC
    GTATCTGCAG CTGGAAGCTC TATAGCCACG GCCAAAGCTG CCACCCCACC CTCAAAGCCG
    CCTCTCAGTC GGGTACCGGT GACCACGGCC GGGGCAGAGA CCCCAGCAGG CAGTCTCCCT
    GGTGAACACC TGCCTCCTCC TTCTCTCCCA GGACCTTCAC TAACTCAGTC CCAGCAGCCT
    CTAGAAGACC TGGATTTCCA GCTGAGAAGG ACGTTGAGCC CTGACACTGT CCCTGTGCCT
    TCTGCACTAA TATTTCCTGC ACACAGCGGA GCTTCTACCG GTGTTGCAGC ACCCGTCGGC
    TCAGCATTGC AACCCTCCGC TTCAGAATTG CCAAAAGCCA GCGCTGGAGA GGCAGAGGTG
    GGCGCGAGTG TCCAGAGGAT GGGCAGGTTT CAGGTCTCTT TGGCAGCAGA GAATGTGCTC
    AAGCAGAGCG AGGAGACCAA ACTGTCCATC TCAGACACTT CCTCCACCAC TTCTACATCC
    TCCTCTTCCT CTGCGTCATG CAGCAGCCCA GAGAGCACCC TGACTAAGCC CCAGGCCAAA
    ACCGCTGCTC CCTTAGACAT AGTGGATGGT GTTGGTCCCC ACTTGGTGCA TCTACAGAGC
    CTATCTCCCA AGCAGTTGGT GAAAGTGGGG CGCTTCCAGG TTACCACTGC CACCACGGAC
    AAAGTGGGGC GCTTTTCTGT GTCCCGGACA CAAGATGATA TATCTGCTGG GGTGCTGGAG
    ACAGACTCCC AGAGGTCCTC CTCCCCACAG ACTGAGAAAC ATCTGAATGG ACCCTACTTA
    CAGCAAGACT CCTCTGCGGC TCTCGGCTAT CCGGCCAGGG AGACGCAGGA AGACAGCCTA
    GGGAGCCCCG TTCTGCACCA CGCTTTGGGG TCCAAGCTTA GCCTGCAGAG CCTTAGCAAC
    TCGTTCAACT CTTCCTACAT GAGCAGCGAT AATGATTCTG ACATAGAAGA TGAAGATCTG
    AAGAAGGAGC TGCGAAGGCT ACGAGACAAG CATCTCAAGG AGATTCAGGA ACTGCAGAGT
    CGACAGAAGC AGGAGATCGA GCTGTTGTAC ACGGCTTTGG GAAAAGTGCC CCCTGCTGTC
    ATTATCCCTC CGGCAGCGCC CCTTTCTGGC CGGCGCAGGA GGCATACCAA GGGGAAGGGC
    AGCAAGTCCA GCCGGGGCAG CTCCCACAAC AGCCGCAGCC CTCATTTGTC AGGCGACCTG
    TCCACGCAGA GCGCTCCGTC GGCATTACCC CCCCAGCCGG GCCTTCTGCT CCCCCCAAGC
    AGTGCGCCCG AACATGCTCA TCTTCTCAAG CCCTCCCCTT CCAGCGACAT TCTCTGCTCA
    GCCTTCACTA GCGACGCTGC CCTCTCTGTG CCCAGCCTGT CCACCCAGGG CCAAGGCTGG
    GCCAAGTTTA ACTGTGAGTC AGAGCGGGTA TCCTTCAAAA TGGCTGGCAG GAGGACGCGA
    TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA GGACCAGCAG CACTAACACA GTCGCACCAT CAACTGCCCC GGCCCCCCAG
    CCAAACGTGC CCCCCGTGTC ATCCAGCCGC AAGGGAACCT TCACGGACGA TCTACACAAA
    CTGGTGGACA ACTGGGCCCG AGACGCCATG AACCTGACTG GAAAAAAGGC TTGTAAGGCC
    TCTGGGCAAC CCAGCTATGA GGGACCCGGG ATGGCCCGTA AGTTCTCTGC TCCCGGACAG
    CTGTGTGTCC CCATGAACCC ATCCCTGGGG GGCTCCGTCC CTCTCCCAGC AGCTTCGGCG
    ACCTCCCTGG CTCACTACCC CAAAGGAATG TGCCCCCCGC AACAATACGG AATCCCCGGG
    GCGCTGTTCC AAGCTCCATG GAGTGGCACC GGCGTGCCCC CCTCCTCTCA GCCCCTAAGC
    CAGTTCCCCC CGGCGGTGAC ATCTTCCTTA CAGAACTTCG GTATTAGCAG CTTGCAGAAA
    TCTATCAGCA ACCCCCAAGG ATCCAACCTG CGGACTACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754487.1
    CDS130..7431
    Translation

    Target ORF information:

    RefSeq Version XM_031898627.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCCCCAG AGATGTATGA GGAGAAATAC GATGAGTCCG TTGACGTGTA TGCGTTTGGG 
    ATGTGCATGC TTGAGATGGC CACTTCTGAA TACCCTTACT CCGAATGCCA AAACGCTGCA
    CAGATCTACC GGCGAGTCAC CAGTGGCGTG AAGCCTGCCA GCTTCGATAA AGTCGCAATT
    CCAGAGGTGA AAGAAATCAT CGAAGGGTGC ATTCGCCAAA ACAAGGACGA AAGGTATGCC
    ATTAAGGACC TGCTGAACCA TGCCTTTTTC CAAGAGGAAA CTGGGGTCCG CGTGGAACTG
    GCAGAGGAAG ATGACGGGGA AAAGATTGCT ATCAAGCTTT GGCTGCGTAT TGAGGATATC
    AAAAAACTCA AGGGCAAATA CAAGGACAAT GAGGCAATTG AGTTCTCCTT TGACCTGGAG
    CGAGATGTTC CGGATGATGT GGCACAGGAA ATGGTGGAAT CTGGCTACGT CTGTGAAGGA
    GACCACAAGA CTATGGCAAA GGCAATCAAA GATAGGGTGT CCCTGATCAA ACGCAAGCGG
    GAACAGAGGC AGCAAGTGCG GGAAGAGCAG GAGAAGAGGA AACAGGAGGA GATTAGCCAG
    CAGCAGCAGC AGCAACAAGC AGAGCTTCAG CCTCAGAGTC CTCCTCAAGG CACCGTCAAA
    CCCTCCACTG GCACCGTTAC CTCTGGCACA CAAGTAGAAC CCGAGGAGCC GGAAGCTGAT
    CAGCATCTAC AGTACCAGCA GCCAAGTGTA TCCGTCACAT CATTAGCCGA TGGGACAATT
    GACAGTGGTC AGGGATCGTC CATGTATTCT GAATCTTATG CGGGAAACCA GCCGACAGTC
    TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC ACTCCCAGCA TGGTGCCTGG CTTCAGCACT
    CCAAATATCC AACCCCCTGC CCAGCATGGC GGATACCCAG CTCCTGTAGG GCCTTGTCGA
    GGTCGTAGCA TGTCAGTATG TGTTCCCAAC CTTTCCTCTG CTCCCCCACT CCTTTCCGTT
    GTGCCCCCCA GTGCTCCAGG CTCCCCTTCC CCATTGCAGT CTGCCCCTGC CTCCCCACTC
    TGGACATCTC CCGAAGATTC CTTTGCTGAA AAGCTTTCCA AGGCTCTAGA GAGTGTTCTG
    CCCATGCACT CCTCCAGCCC TCGCAAGCAC CAACGCCGCT GCAGCCTGCC TACCCTCTAT
    ACCAGCCCTC CATCGTCCAT GGTCCATTCA GCACTCCCAG ACTCTCCGCT TTTTTTCCCA
    ACCATTCCCA ACCAGCGCCC CATGTCTTTC TCTCCGCCGC CTACTGGCTT GTCCCCAAAG
    GTGCCTGCAA CCCAGCGACG CAAGAGCACC TCTTTCTTGG AGTCCCAAGG CCGCCAGGGG
    CTATCTCAGA TTAGGACACT CGGGCCAGGT GAGGAGACCC AGTGCTGGAG GGCAGACTAC
    ATGGCAGCTC TAGGTAAAGC ATCTGTCCGA TCCGAGGATG CTCACAAGCA TCAAAACTAT
    GAGGGATTGG CGTTCCATGA CGCAAGACAT GTTCCGACAG TTCCAAATGA AACTCTCTTA
    AAGCCTGGGG AAGCATTGTA TCTAGCCAGT CTGCCCTCAC AGGAGAGCTA CAGCAGCATG
    GCTGCCCCTG AGCATATGGA GGGCCACAAG CTGACCCAGC TTGGCTCGTT TTACGATTAT
    CACACTGGGC AAACCTACAT TGCGCGGCCC CTGAAGAGCC TAAGACTGGA ATCTGGGTTA
    AACCAGCCGT CGGCTTTGGG TAGCATCTCC AACCCAATCT CCTCCGACCC ACACGCCATG
    TTTCATCAGA TGTTTGTGCC CCAGTCTGCT CCTCCAGTGC TGGCTCAAAG CAGCGAAGGC
    CACCCAGGGT GTGTGTTTGA GTTCCACGTG CACCCATCTG GGTCTGTTCC CGGCGAAGCA
    ACCGTTTACT TGCCACACCG AGTTTACCGT CCTCGACGGG GCTCTGTAGA CTTTAACCCA
    GAAGAACAGC CGCCGGGTTC TACGCTGCAG AGCCGCCTCC AAACTGTGAC TGAAGAGCAG
    TGCCACCACA ATGGCCCAGA ACCACTCCCA CCCTTTATAG GATTCATCAC ATGCAACCGT
    ATGGAGAGCA GCCCCGACTA TTCCAGTGAT TCTTCCCAAA GGAATTCATC CGATCCCGAT
    TATCTCTCTC CTCCTCTGGC CTTCAGCTCC AATATCTCTA TGGCTTCCAA GCAGAAAACG
    TTGCAGGATC CACAGATTTT CTTTTGTGTC CCACAGTCCG GAACGACGGC TTATGCTGGA
    CCATCAGCTT TGTACAGCCA GCAGCCACAA ATATCATCTG GACAACCACC CACCACATCC
    ATGGGAATCA GTGCTCAGCC CGGTCCCCAC ACCCAGGGCA TGGTGCATCA TCAGCCTTCT
    CTGCATCCAG CCTCGCAGTA CCAAGTGCAG CAGCCAATGC CTACTGTGGG TTCCAGTATT
    GCGGCCCCAT CACAGCCGGT GATTACCCAA ACGCCAGCCC CTCAGCCGGG CACCCAGCTC
    CCTGCCTCCC AGCCTTTGCC TGCTGTTCAA GGAGAGCCTC AGATTCCTGC TCAGACAGCT
    CCTGCTCCCC AGCCCGTTCC TACAGTGCCG GCCTCTCTGC CACCTCATTT TCTTCCCTTG
    GCACAATCTT TGGTTGCAGT CCCTCTCATT CCTCCATTCG CTGCCCCACA GTTGCCTTTA
    ACGGTACCAC CCCCGGTCAT ACAGACAGGG TTCCCTTCCC TACCCATGAC TGTAACCCCA
    AGTATTGGCC AAACTGTACC AGGAATACCC CCATCCTTAG GAGCGCCAGT CCTGCCCCAT
    GGGCAGGCAG TGTTAGTTCC CACTCACATC ACTAATGTTG CTCTCCAGCC TTTGGCACCA
    ATCCCCACAC CTCCTCTGTG CCCTGTAACT CAGCCCATTG GATTACCGGC CACCCTGGGA
    AAAGCTGCTG AGCCCTTGCT TCCTCCGGGA GATACTTCTT ACCAGGTTTT CCAGGCTACT
    CACCCTCTAT TCCCTGGAGA TGTAAGCTTT GTTCCTCCCG TTGCCATCCC CTCAGCAATG
    GTATCTCCCC CTATCCGTGG TGAAAGTTTA GCCCAACCTG GCTTCCATAC TCAGCCTTAT
    CCTGAAAACA GCAATTTGTA TTCCCATGGA GGGCCCACTC CCCAGCCCGC TGTTCTCACT
    ACCCAGCCGG TGCCTGCAGG AACCCAGACG CAACCTGCCG TCGAGGGTTC ACAGAGTAGC
    TCCCTGGCAA TTTCCCAGGA TACCCAGTCA TCTCCTCAAG CAACACAAAC TGCCTCCTCC
    ATGGACAGCG CTCATTCAGA CGTGGCATCA GGACTGAGTG ACGGAAACGA GGGCAGCACA
    GGTGGGAGAC ACGAAGGCCG AGCCACTAAA CGACACTACC GAAAATCTGT GAGAAGCCGC
    TCCAGGCACG AGAAAACTGC ACGCCCAAAG CTTCGAATCC TTAATGTTTC CAACAAAGGG
    GATCGTGTTG TAGAGTGTCA GCTGGAGACT CACAACCGCA AGATGGTCAC CTTCAAATTC
    GACCTGGATG GAGACAATCC AGAGGAGATT GCTACTATAA TGGTACAGAA TGTGTTTATT
    TTGGCTACGG AGAGAGAATC CTTTGTAGAG CAGGTAAAAG ACATAATTGA GAAGGCCGAT
    GAAATGTTGA GCGAAGATGT GAGCGTGGAG CCGGAAGGAG AAACGAGGGC AGAAAGTTTG
    CAAATCAAGG ATGATGGGGA TTACCCAGAA TCTCAGAAGA TGAATTCTGA ATTTCGAATG
    CCAGATCCTG TATATTCCCT ACCACAGACA GCAGGGCTTA TTCCCAGCGT TGTCACACAA
    GTCGTTCATT CTGCCGGAAG ACGATTTATT GTCAGTCCGG TGCCTGAAAG CCGTCTAAAA
    GAACAGTCAT TCTTCAGTAC TTCATTGCAG GACCCTCAGC TACCAGTCTC TGTCGCGCAT
    GGTCCAGGGA TGAATTTGTC TCATTCTGCC TCTTCTGTGA GCCTGCAGCA AGCCTTTGCA
    GAACTACGCC AGTCACAGAT GAGCGAGGGT CCTAGCACAG CTCCTCCCAT ATTTAACCAA
    ACTGGACCCC CCTTTCCACC ATCCATGACT AGTCTGGTGG CAACTCTGCC TTCTAATGCA
    ATGCCTATCC TCCAGGCAGC CCCAGTGATC CCCAGTAGCC TGCCATCTTC TGTGCCTCAG
    CCCCTGGTGG AGGTGGTTGT ACCTCAGGCT GCTGGGATGG CTCCAACTAG CAATGTTCCA
    AGCACCTTGT CCCCTCCCCT AACTGCAGTA TCCAACCAGC ACGCAGACAT GATACCGGGT
    GCTGCTGTAG CAGCTATCGG ATCACTTCCT GTGACATCAC TTCCTCCTTT GCCCATTGTG
    GGTGGAGTTC CTGTAGGCAC TGCAACCCTG AGCTCACCCC TAACACCCCT AATCAGTTCT
    GTCAGTACAA TGGGGGGGAC GGTTCCCCCA TCTGTCACAT CTCCCCCAGT GCCACAGATA
    GCGGGTAGCG TTGCCGGGAG CCTGCCTCCT GCATCAGCCT CCGCCCCTCT TATTCATGGC
    AGTGTTGCCC CATCTCAGGT ACCGCTTTGC AGCAGCAGTT CTGTTCCCAG CTTTGCAGAG
    AGCGTGGCAG TCAGCCCCCT GCTGGATAGT GGCCAAAAGG CAGCAGAAGT GGGCACGGTA
    ATGAGCACGA CTGGCTTGTC TCTGCCCATC TCTGCACCTC TGACATATGC TGGGGTGGTT
    GCAGCATCAG GCATCACAAG TCAACCTGCC GTGCATCTCC CCCAGGGGGT GGCCACAACA
    CCAGTTGTAT CCCAGGCAGT AGGGGGAGCT GTGGGCACAC CCCTCTCATC TCTTCCTCCC
    ATCCCTGGAG GCATGTCTGT TAGCCAACCA GGATCAAATA TACCCCCTGT GCAACACACG
    TTGGCCCATG GCCAACCCCA GCCTACCCCT CTCCCCAACC AGCCTCATAC GCACCTTGTA
    GAGGGTGACA CCGATAACCT GCCCAAACCT GGCATAGACG ATATAAAAAC CCTGGAGGAG
    AAGCTGAGGT CGCTGTTCAG TGAGCATGGC AGTGCCGGGG CCCCTCATGG ACTCACCCCA
    TCAGAAACTC AATTGGGTTT GGATGGGGCC CCCCTGCCAG GCCCTTCCAC AGGTAGCACC
    GCTCAGACCA AGCCTGCAGC ATCCTCTTCT CCACCAACCA GCTTACCTTT AGGGCCACCT
    GGAGTGACGA CAATGACTCA GATCATGACA CCTGGACAAG CTGCTACACC TGTCACCTAC
    GTATCTGCAG CTGGAAGCTC TATAGCCACG GCCAAAGCTG CCACCCCACC CTCAAAGCCG
    CCTCTCAGTC GGGTACCGGT GACCACGGCC GGGGCAGAGA CCCCAGCAGG CAGTCTCCCT
    GGTGAACACC TGCCTCCTCC TTCTCTCCCA GGACCTTCAC TAACTCAGTC CCAGCAGCCT
    CTAGAAGACC TGGATTTCCA GCTGAGAAGG ACGTTGAGCC CTGACACTGT CCCTGTGCCT
    TCTGCACTAA TATTTCCTGC ACACAGCGGA GCTTCTACCG GTGTTGCAGC ACCCGTCGGC
    TCAGCATTGC AACCCTCCGC TTCAGAATTG CCAAAAGCCA GCGCTGGAGA GGCAGAGGTG
    GGCGCGAGTG TCCAGAGGAT GGGCAGGTTT CAGGTCTCTT TGGCAGCAGA GAATGTGCTC
    AAGCAGAGCG AGGAGACCAA ACTGTCCATC TCAGACACTT CCTCCACCAC TTCTACATCC
    TCCTCTTCCT CTGCGTCATG CAGCAGCCCA GAGAGCACCC TGACTAAGCC CCAGGCCAAA
    ACCGCTGCTC CCTTAGACAT AGTGGATGGT GTTGGTCCCC ACTTGGTGCA TCTACAGAGC
    CTATCTCCCA AGCAGTTGGT GAAAGTGGGG CGCTTCCAGG TTACCACTGC CACCACGGAC
    AAAGTGGGGC GCTTTTCTGT GTCCCGGACA CAAGATGATA TATCTGCTGG GGTGCTGGAG
    ACAGACTCCC AGAGGTCCTC CTCCCCACAG ACTGAGAAAC ATCTGAATGG ACCCTACTTA
    CAGCAAGACT CCTCTGCGGC TCTCGGCTAT CCGGCCAGGG AGACGCAGGA AGACAGCCTA
    GGGAGCCCCG TTCTGCACCA CGCTTTGGGG TCCAAGCTTA GCCTGCAGAG CCTTAGCAAC
    TCGTTCAACT CTTCCTACAT GAGCAGCGAT AATGATTCTG ACATAGAAGA TGAAGATCTG
    AAGAAGGAGC TGCGAAGGCT ACGAGACAAG CATCTCAAGG AGATTCAGGA ACTGCAGAGT
    CGACAGAAGC AGGAGATCGA GCTGTTGTAC ACGGCTTTGG GAAAAGTGCC CCCTGCTGTC
    ATTATCCCTC CGGCAGCGCC CCTTTCTGGC CGGCGCAGGA GGCATACCAA GGGGAAGGGC
    AGCAAGTCCA GCCGGGGCAG CTCCCACAAC AGCCGCAGCC CTCATTTGTC AGGCGACCTG
    TCCACGCAGA GCGCTCCGTC GGCATTACCC CCCCAGCCGG GCCTTCTGCT CCCCCCAAGC
    AGTGCGCCCG AACATGCTCA TCTTCTCAAG CCCTCCCCTT CCAGCGACAT TCTCTGCTCA
    GCCTTCACTA GCGACGCTGC CCTCTCTGTG CCCAGCCTGT CCACCCAGGG CCAAGGCTGG
    GCCAAGTTTA ACTGTGAGTC AGAGCGGGTA TCCTTCAAAA TGGCTGGCAG GAGGACGCGA
    TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA GGACCAGCAG CACTAACACA GTCGCACCAT CAACTGCCCC GGCCCCCCAG
    CCAAACGTGC CCCCCGTGTC ATCCAGCCGC AAGGGAACCT TCACGGACGA TCTACACAAA
    CTGGTGGACA ACTGGGCCCG AGACGCCATG AACCTGACTG GAAAAAAGGC TTGTAAGGCC
    TCTGGGCAAC CCAGCTATGA GGGACCCGGG ATGGCCCGTA AGTTCTCTGC TCCCGGACAG
    CTGTGTGTCC CCATGAACCC ATCCCTGGGG GGCTCCGTCC CTCTCCCAGC AGCTTCGGCG
    ACCTCCCTGG CTCACTACCC CAAAGGAATG TGCCCCCCGC AACAATACGG AATCCCCGGG
    GCGCTGTTCC AAGCTCCATG GAGTGGCACC GGCGTGCCCC CCTCCTCTCA GCCCCTAAGC
    CAGTTCCCCC CGGCGGTGAC ATCTTCCTTA CAGAACTTCG GTATTAGCAG CTTGCAGAAA
    TCTATCAGCA ACCCCCAAGG ATCCAACCTG CGGACTACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54269
    Clone ID Related Accession (Same CDS sequence) XM_031898628.1
    Accession Version XM_031898628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGATTATA CCATGAAGGA TTTTTGCCCA GTGGTATTTG TAGTCCATTC CCACTGCTGT 
    TGCCTTACCC ATGACTGCCC TCAGGGCGTG AAGCCTGCCA GCTTCGATAA AGTCGCAATT
    CCAGAGGTGA AAGAAATCAT CGAAGGGTGC ATTCGCCAAA ACAAGGACGA AAGGTATGCC
    ATTAAGGACC TGCTGAACCA TGCCTTTTTC CAAGAGGAAA CTGGGGTCCG CGTGGAACTG
    GCAGAGGAAG ATGACGGGGA AAAGATTGCT ATCAAGCTTT GGCTGCGTAT TGAGGATATC
    AAAAAACTCA AGGGCAAATA CAAGGACAAT GAGGCAATTG AGTTCTCCTT TGACCTGGAG
    CGAGATGTTC CGGATGATGT GGCACAGGAA ATGGTGGAAT CTGGCTACGT CTGTGAAGGA
    GACCACAAGA CTATGGCAAA GGCAATCAAA GATAGGGTGT CCCTGATCAA ACGCAAGCGG
    GAACAGAGGC AGCAAGTGCG GGAAGAGCAG GAGAAGAGGA AACAGGAGGA GATTAGCCAG
    CAGCAGCAGC AGCAACAAGC AGAGCTTCAG CCTCAGAGTC CTCCTCAAGG CACCGTCAAA
    CCCTCCACTG GCACCGTTAC CTCTGGCACA CAAGTAGAAC CCGAGGAGCC GGAAGCTGAT
    CAGCATCTAC AGTACCAGCA GCCAAGTGTA TCCGTCACAT CATTAGCCGA TGGGACAATT
    GACAGTGGTC AGGGATCGTC CATGTATTCT GAATCTTATG CGGGAAACCA GCCGACAGTC
    TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC ACTCCCAGCA TGGTGCCTGG CTTCAGCACT
    CCAAATATCC AACCCCCTGC CCAGCATGGC GGATACCCAG CTCCTGTAGG GCCTTGTCGA
    GGTCGTAGCA TGTCAGTATG TGTTCCCAAC CTTTCCTCTG CTCCCCCACT CCTTTCCGTT
    GTGCCCCCCA GTGCTCCAGG CTCCCCTTCC CCATTGCAGT CTGCCCCTGC CTCCCCACTC
    TGGACATCTC CCGAAGATTC CTTTGCTGAA AAGCTTTCCA AGGCTCTAGA GAGTGTTCTG
    CCCATGCACT CCTCCAGCCC TCGCAAGCAC CAACGCCGCT GCAGCCTGCC TACCCTCTAT
    ACCAGCCCTC CATCGTCCAT GGTCCATTCA GCACTCCCAG ACTCTCCGCT TTTTTTCCCA
    ACCATTCCCA ACCAGCGCCC CATGTCTTTC TCTCCGCCGC CTACTGGCTT GTCCCCAAAG
    GTGCCTGCAA CCCAGCGACG CAAGAGCACC TCTTTCTTGG AGTCCCAAGG CCGCCAGGGG
    CTATCTCAGA TTAGGACACT CGGGCCAGGT GAGGAGACCC AGTGCTGGAG GGCAGACTAC
    ATGGCAGCTC TAGGTAAAGC ATCTGTCCGA TCCGAGGATG CTCACAAGCA TCAAAACTAT
    GAGGGATTGG CGTTCCATGA CGCAAGACAT GTTCCGACAG TTCCAAATGA AACTCTCTTA
    AAGCCTGGGG AAGCATTGTA TCTAGCCAGT CTGCCCTCAC AGGAGAGCTA CAGCAGCATG
    GCTGCCCCTG AGCATATGGA GGGCCACAAG CTGACCCAGC TTGGCTCGTT TTACGATTAT
    CACACTGGGC AAACCTACAT TGCGCGGCCC CTGAAGAGCC TAAGACTGGA ATCTGGGTTA
    AACCAGCCGT CGGCTTTGGG TAGCATCTCC AACCCAATCT CCTCCGACCC ACACGCCATG
    TTTCATCAGA TGTTTGTGCC CCAGTCTGCT CCTCCAGTGC TGGCTCAAAG CAGCGAAGGC
    CACCCAGGGT GTGTGTTTGA GTTCCACGTG CACCCATCTG GGTCTGTTCC CGGCGAAGCA
    ACCGTTTACT TGCCACACCG AGTTTACCGT CCTCGACGGG GCTCTGTAGA CTTTAACCCA
    GAAGAACAGC CGCCGGGTTC TACGCTGCAG AGCCGCCTCC AAACTGTGAC TGAAGAGCAG
    TGCCACCACA ATGGCCCAGA ACCACTCCCA CCCTTTATAG GATTCATCAC ATGCAACCGT
    ATGGAGAGCA GCCCCGACTA TTCCAGTGAT TCTTCCCAAA GGAATTCATC CGATCCCGAT
    TATCTCTCTC CTCCTCTGGC CTTCAGCTCC AATATCTCTA TGGCTTCCAA GCAGAAAACG
    TTGCAGGATC CACAGATTTT CTTTTGTGTC CCACAGTCCG GAACGACGGC TTATGCTGGA
    CCATCAGCTT TGTACAGCCA GCAGCCACAA ATATCATCTG GACAACCACC CACCACATCC
    ATGGGAATCA GTGCTCAGCC CGGTCCCCAC ACCCAGGGCA TGGTGCATCA TCAGCCTTCT
    CTGCATCCAG CCTCGCAGTA CCAAGTGCAG CAGCCAATGC CTACTGTGGG TTCCAGTATT
    GCGGCCCCAT CACAGCCGGT GATTACCCAA ACGCCAGCCC CTCAGCCGGG CACCCAGCTC
    CCTGCCTCCC AGCCTTTGCC TGCTGTTCAA GGAGAGCCTC AGATTCCTGC TCAGACAGCT
    CCTGCTCCCC AGCCCGTTCC TACAGTGCCG GCCTCTCTGC CACCTCATTT TCTTCCCTTG
    GCACAATCTT TGGTTGCAGT CCCTCTCATT CCTCCATTCG CTGCCCCACA GTTGCCTTTA
    ACGGTACCAC CCCCGGTCAT ACAGACAGGG TTCCCTTCCC TACCCATGAC TGTAACCCCA
    AGTATTGGCC AAACTGTACC AGGAATACCC CCATCCTTAG GAGCGCCAGT CCTGCCCCAT
    GGGCAGGCAG TGTTAGTTCC CACTCACATC ACTAATGTTG CTCTCCAGCC TTTGGCACCA
    ATCCCCACAC CTCCTCTGTG CCCTGTAACT CAGCCCATTG GATTACCGGC CACCCTGGGA
    AAAGCTGCTG AGCCCTTGCT TCCTCCGGGA GATACTTCTT ACCAGGTTTT CCAGGCTACT
    CACCCTCTAT TCCCTGGAGA TGTAAGCTTT GTTCCTCCCG TTGCCATCCC CTCAGCAATG
    GTATCTCCCC CTATCCGTGG TGAAAGTTTA GCCCAACCTG GCTTCCATAC TCAGCCTTAT
    CCTGAAAACA GCAATTTGTA TTCCCATGGA GGGCCCACTC CCCAGCCCGC TGTTCTCACT
    ACCCAGCCGG TGCCTGCAGG AACCCAGACG CAACCTGCCG TCGAGGGTTC ACAGAGTAGC
    TCCCTGGCAA TTTCCCAGGA TACCCAGTCA TCTCCTCAAG CAACACAAAC TGCCTCCTCC
    ATGGACAGCG CTCATTCAGA CGTGGCATCA GGACTGAGTG ACGGAAACGA GGGCAGCACA
    GGTGGGAGAC ACGAAGGCCG AGCCACTAAA CGACACTACC GAAAATCTGT GAGAAGCCGC
    TCCAGGCACG AGAAAACTGC ACGCCCAAAG CTTCGAATCC TTAATGTTTC CAACAAAGGG
    GATCGTGTTG TAGAGTGTCA GCTGGAGACT CACAACCGCA AGATGGTCAC CTTCAAATTC
    GACCTGGATG GAGACAATCC AGAGGAGATT GCTACTATAA TGGTACAGAA TGTGTTTATT
    TTGGCTACGG AGAGAGAATC CTTTGTAGAG CAGGTAAAAG ACATAATTGA GAAGGCCGAT
    GAAATGTTGA GCGAAGATGT GAGCGTGGAG CCGGAAGGAG AAACGAGGGC AGAAAGTTTG
    CAAATCAAGG ATGATGGGGA TTACCCAGAA TCTCAGAAGA TGAATTCTGA ATTTCGAATG
    CCAGATCCTG TATATTCCCT ACCACAGACA GCAGGGCTTA TTCCCAGCGT TGTCACACAA
    GTCGTTCATT CTGCCGGAAG ACGATTTATT GTCAGTCCGG TGCCTGAAAG CCGTCTAAAA
    GAACAGTCAT TCTTCAGTAC TTCATTGCAG GACCCTCAGC TACCAGTCTC TGTCGCGCAT
    GGTCCAGGGA TGAATTTGTC TCATTCTGCC TCTTCTGTGA GCCTGCAGCA AGCCTTTGCA
    GAACTACGCC AGTCACAGAT GAGCGAGGGT CCTAGCACAG CTCCTCCCAT ATTTAACCAA
    ACTGGACCCC CCTTTCCACC ATCCATGACT AGTCTGGTGG CAACTCTGCC TTCTAATGCA
    ATGCCTATCC TCCAGGCAGC CCCAGTGATC CCCAGTAGCC TGCCATCTTC TGTGCCTCAG
    CCCCTGGTGG AGGTGGTTGT ACCTCAGGCT GCTGGGATGG CTCCAACTAG CAATGTTCCA
    AGCACCTTGT CCCCTCCCCT AACTGCAGTA TCCAACCAGC ACGCAGACAT GATACCGGGT
    GCTGCTGTAG CAGCTATCGG ATCACTTCCT GTGACATCAC TTCCTCCTTT GCCCATTGTG
    GGTGGAGTTC CTGTAGGCAC TGCAACCCTG AGCTCACCCC TAACACCCCT AATCAGTTCT
    GTCAGTACAA TGGGGGGGAC GGTTCCCCCA TCTGTCACAT CTCCCCCAGT GCCACAGATA
    GCGGGTAGCG TTGCCGGGAG CCTGCCTCCT GCATCAGCCT CCGCCCCTCT TATTCATGGC
    AGTGTTGCCC CATCTCAGGT ACCGCTTTGC AGCAGCAGTT CTGTTCCCAG CTTTGCAGAG
    AGCGTGGCAG TCAGCCCCCT GCTGGATAGT GGCCAAAAGG CAGCAGAAGT GGGCACGGTA
    ATGAGCACGA CTGGCTTGTC TCTGCCCATC TCTGCACCTC TGACATATGC TGGGGTGGTT
    GCAGCATCAG GCATCACAAG TCAACCTGCC GTGCATCTCC CCCAGGGGGT GGCCACAACA
    CCAGTTGTAT CCCAGGCAGT AGGGGGAGCT GTGGGCACAC CCCTCTCATC TCTTCCTCCC
    ATCCCTGGAG GCATGTCTGT TAGCCAACCA GGATCAAATA TACCCCCTGT GCAACACACG
    TTGGCCCATG GCCAACCCCA GCCTACCCCT CTCCCCAACC AGCCTCATAC GCACCTTGTA
    GAGGGTGACA CCGATAACCT GCCCAAACCT GGCATAGACG ATATAAAAAC CCTGGAGGAG
    AAGCTGAGGT CGCTGTTCAG TGAGCATGGC AGTGCCGGGG CCCCTCATGG ACTCACCCCA
    TCAGAAACTC AATTGGGTTT GGATGGGGCC CCCCTGCCAG GCCCTTCCAC AGGTAGCACC
    GCTCAGACCA AGCCTGCAGC ATCCTCTTCT CCACCAACCA GCTTACCTTT AGGGCCACCT
    GGAGTGACGA CAATGACTCA GATCATGACA CCTGGACAAG CTGCTACACC TGTCACCTAC
    GTATCTGCAG CTGGAAGCTC TATAGCCACG GCCAAAGCTG CCACCCCACC CTCAAAGCCG
    CCTCTCAGTC GGGTACCGGT GACCACGGCC GGGGCAGAGA CCCCAGCAGG CAGTCTCCCT
    GGTGAACACC TGCCTCCTCC TTCTCTCCCA GGACCTTCAC TAACTCAGTC CCAGCAGCCT
    CTAGAAGACC TGGATTTCCA GCTGAGAAGG ACGTTGAGCC CTGACACTGT CCCTGTGCCT
    TCTGCACTAA TATTTCCTGC ACACAGCGGA GCTTCTACCG GTGTTGCAGC ACCCGTCGGC
    TCAGCATTGC AACCCTCCGC TTCAGAATTG CCAAAAGCCA GCGCTGGAGA GGCAGAGGTG
    GGCGCGAGTG TCCAGAGGAT GGGCAGGTTT CAGGTCTCTT TGGCAGCAGA GAATGTGCTC
    AAGCAGAGCG AGGAGACCAA ACTGTCCATC TCAGACACTT CCTCCACCAC TTCTACATCC
    TCCTCTTCCT CTGCGTCATG CAGCAGCCCA GAGAGCACCC TGACTAAGCC CCAGGCCAAA
    ACCGCTGCTC CCTTAGACAT AGTGGATGGT GTTGGTCCCC ACTTGGTGCA TCTACAGAGC
    CTATCTCCCA AGCAGTTGGT GAAAGTGGGG CGCTTCCAGG TTACCACTGC CACCACGGAC
    AAAGTGGGGC GCTTTTCTGT GTCCCGGACA CAAGATGATA TATCTGCTGG GGTGCTGGAG
    ACAGACTCCC AGAGGTCCTC CTCCCCACAG ACTGAGAAAC ATCTGAATGG ACCCTACTTA
    CAGCAAGACT CCTCTGCGGC TCTCGGCTAT CCGGCCAGGG AGACGCAGGA AGACAGCCTA
    GGGAGCCCCG TTCTGCACCA CGCTTTGGGG TCCAAGCTTA GCCTGCAGAG CCTTAGCAAC
    TCGTTCAACT CTTCCTACAT GAGCAGCGAT AATGATTCTG ACATAGAAGA TGAAGATCTG
    AAGAAGGAGC TGCGAAGGCT ACGAGACAAG CATCTCAAGG AGATTCAGGA ACTGCAGAGT
    CGACAGAAGC AGGAGATCGA GCTGTTGTAC ACGGCTTTGG GAAAAGTGCC CCCTGCTGTC
    ATTATCCCTC CGGCAGCGCC CCTTTCTGGC CGGCGCAGGA GGCATACCAA GGGGAAGGGC
    AGCAAGTCCA GCCGGGGCAG CTCCCACAAC AGCCGCAGCC CTCATTTGTC AGGCGACCTG
    TCCACGCAGA GCGCTCCGTC GGCATTACCC CCCCAGCCGG GCCTTCTGCT CCCCCCAAGC
    AGTGCGCCCG AACATGCTCA TCTTCTCAAG CCCTCCCCTT CCAGCGACAT TCTCTGCTCA
    GCCTTCACTA GCGACGCTGC CCTCTCTGTG CCCAGCCTGT CCACCCAGGG CCAAGGCTGG
    GCCAAGTTTA ACTGTGAGTC AGAGCGGGTA TCCTTCAAAA TGGCTGGCAG GAGGACGCGA
    TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA GGACCAGCAG CACTAACACA GTCGCACCAT CAACTGCCCC GGCCCCCCAG
    CCAAACGTGC CCCCCGTGTC ATCCAGCCGC AAGGGAACCT TCACGGACGA TCTACACAAA
    CTGGTGGACA ACTGGGCCCG AGACGCCATG AACCTGACTG GAAAAAAGGC TTGTAAGGCC
    TCTGGGCAAC CCAGCTATGA GGGACCCGGG ATGGCCCGTA AGTTCTCTGC TCCCGGACAG
    CTGTGTGTCC CCATGAACCC ATCCCTGGGG GGCTCCGTCC CTCTCCCAGC AGCTTCGGCG
    ACCTCCCTGG CTCACTACCC CAAAGGAATG TGCCCCCCGC AACAATACGG AATCCCCGGG
    GCGCTGTTCC AAGCTCCATG GAGTGGCACC GGCGTGCCCC CCTCCTCTCA GCCCCTAAGC
    CAGTTCCCCC CGGCGGTGAC ATCTTCCTTA CAGAACTTCG GTATTAGCAG CTTGCAGAAA
    TCTATCAGCA ACCCCCAAGG ATCCAACCTG CGGACTACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754488.1
    CDS151..7392
    Translation

    Target ORF information:

    RefSeq Version XM_031898628.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGATTATA CCATGAAGGA TTTTTGCCCA GTGGTATTTG TAGTCCATTC CCACTGCTGT 
    TGCCTTACCC ATGACTGCCC TCAGGGCGTG AAGCCTGCCA GCTTCGATAA AGTCGCAATT
    CCAGAGGTGA AAGAAATCAT CGAAGGGTGC ATTCGCCAAA ACAAGGACGA AAGGTATGCC
    ATTAAGGACC TGCTGAACCA TGCCTTTTTC CAAGAGGAAA CTGGGGTCCG CGTGGAACTG
    GCAGAGGAAG ATGACGGGGA AAAGATTGCT ATCAAGCTTT GGCTGCGTAT TGAGGATATC
    AAAAAACTCA AGGGCAAATA CAAGGACAAT GAGGCAATTG AGTTCTCCTT TGACCTGGAG
    CGAGATGTTC CGGATGATGT GGCACAGGAA ATGGTGGAAT CTGGCTACGT CTGTGAAGGA
    GACCACAAGA CTATGGCAAA GGCAATCAAA GATAGGGTGT CCCTGATCAA ACGCAAGCGG
    GAACAGAGGC AGCAAGTGCG GGAAGAGCAG GAGAAGAGGA AACAGGAGGA GATTAGCCAG
    CAGCAGCAGC AGCAACAAGC AGAGCTTCAG CCTCAGAGTC CTCCTCAAGG CACCGTCAAA
    CCCTCCACTG GCACCGTTAC CTCTGGCACA CAAGTAGAAC CCGAGGAGCC GGAAGCTGAT
    CAGCATCTAC AGTACCAGCA GCCAAGTGTA TCCGTCACAT CATTAGCCGA TGGGACAATT
    GACAGTGGTC AGGGATCGTC CATGTATTCT GAATCTTATG CGGGAAACCA GCCGACAGTC
    TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC ACTCCCAGCA TGGTGCCTGG CTTCAGCACT
    CCAAATATCC AACCCCCTGC CCAGCATGGC GGATACCCAG CTCCTGTAGG GCCTTGTCGA
    GGTCGTAGCA TGTCAGTATG TGTTCCCAAC CTTTCCTCTG CTCCCCCACT CCTTTCCGTT
    GTGCCCCCCA GTGCTCCAGG CTCCCCTTCC CCATTGCAGT CTGCCCCTGC CTCCCCACTC
    TGGACATCTC CCGAAGATTC CTTTGCTGAA AAGCTTTCCA AGGCTCTAGA GAGTGTTCTG
    CCCATGCACT CCTCCAGCCC TCGCAAGCAC CAACGCCGCT GCAGCCTGCC TACCCTCTAT
    ACCAGCCCTC CATCGTCCAT GGTCCATTCA GCACTCCCAG ACTCTCCGCT TTTTTTCCCA
    ACCATTCCCA ACCAGCGCCC CATGTCTTTC TCTCCGCCGC CTACTGGCTT GTCCCCAAAG
    GTGCCTGCAA CCCAGCGACG CAAGAGCACC TCTTTCTTGG AGTCCCAAGG CCGCCAGGGG
    CTATCTCAGA TTAGGACACT CGGGCCAGGT GAGGAGACCC AGTGCTGGAG GGCAGACTAC
    ATGGCAGCTC TAGGTAAAGC ATCTGTCCGA TCCGAGGATG CTCACAAGCA TCAAAACTAT
    GAGGGATTGG CGTTCCATGA CGCAAGACAT GTTCCGACAG TTCCAAATGA AACTCTCTTA
    AAGCCTGGGG AAGCATTGTA TCTAGCCAGT CTGCCCTCAC AGGAGAGCTA CAGCAGCATG
    GCTGCCCCTG AGCATATGGA GGGCCACAAG CTGACCCAGC TTGGCTCGTT TTACGATTAT
    CACACTGGGC AAACCTACAT TGCGCGGCCC CTGAAGAGCC TAAGACTGGA ATCTGGGTTA
    AACCAGCCGT CGGCTTTGGG TAGCATCTCC AACCCAATCT CCTCCGACCC ACACGCCATG
    TTTCATCAGA TGTTTGTGCC CCAGTCTGCT CCTCCAGTGC TGGCTCAAAG CAGCGAAGGC
    CACCCAGGGT GTGTGTTTGA GTTCCACGTG CACCCATCTG GGTCTGTTCC CGGCGAAGCA
    ACCGTTTACT TGCCACACCG AGTTTACCGT CCTCGACGGG GCTCTGTAGA CTTTAACCCA
    GAAGAACAGC CGCCGGGTTC TACGCTGCAG AGCCGCCTCC AAACTGTGAC TGAAGAGCAG
    TGCCACCACA ATGGCCCAGA ACCACTCCCA CCCTTTATAG GATTCATCAC ATGCAACCGT
    ATGGAGAGCA GCCCCGACTA TTCCAGTGAT TCTTCCCAAA GGAATTCATC CGATCCCGAT
    TATCTCTCTC CTCCTCTGGC CTTCAGCTCC AATATCTCTA TGGCTTCCAA GCAGAAAACG
    TTGCAGGATC CACAGATTTT CTTTTGTGTC CCACAGTCCG GAACGACGGC TTATGCTGGA
    CCATCAGCTT TGTACAGCCA GCAGCCACAA ATATCATCTG GACAACCACC CACCACATCC
    ATGGGAATCA GTGCTCAGCC CGGTCCCCAC ACCCAGGGCA TGGTGCATCA TCAGCCTTCT
    CTGCATCCAG CCTCGCAGTA CCAAGTGCAG CAGCCAATGC CTACTGTGGG TTCCAGTATT
    GCGGCCCCAT CACAGCCGGT GATTACCCAA ACGCCAGCCC CTCAGCCGGG CACCCAGCTC
    CCTGCCTCCC AGCCTTTGCC TGCTGTTCAA GGAGAGCCTC AGATTCCTGC TCAGACAGCT
    CCTGCTCCCC AGCCCGTTCC TACAGTGCCG GCCTCTCTGC CACCTCATTT TCTTCCCTTG
    GCACAATCTT TGGTTGCAGT CCCTCTCATT CCTCCATTCG CTGCCCCACA GTTGCCTTTA
    ACGGTACCAC CCCCGGTCAT ACAGACAGGG TTCCCTTCCC TACCCATGAC TGTAACCCCA
    AGTATTGGCC AAACTGTACC AGGAATACCC CCATCCTTAG GAGCGCCAGT CCTGCCCCAT
    GGGCAGGCAG TGTTAGTTCC CACTCACATC ACTAATGTTG CTCTCCAGCC TTTGGCACCA
    ATCCCCACAC CTCCTCTGTG CCCTGTAACT CAGCCCATTG GATTACCGGC CACCCTGGGA
    AAAGCTGCTG AGCCCTTGCT TCCTCCGGGA GATACTTCTT ACCAGGTTTT CCAGGCTACT
    CACCCTCTAT TCCCTGGAGA TGTAAGCTTT GTTCCTCCCG TTGCCATCCC CTCAGCAATG
    GTATCTCCCC CTATCCGTGG TGAAAGTTTA GCCCAACCTG GCTTCCATAC TCAGCCTTAT
    CCTGAAAACA GCAATTTGTA TTCCCATGGA GGGCCCACTC CCCAGCCCGC TGTTCTCACT
    ACCCAGCCGG TGCCTGCAGG AACCCAGACG CAACCTGCCG TCGAGGGTTC ACAGAGTAGC
    TCCCTGGCAA TTTCCCAGGA TACCCAGTCA TCTCCTCAAG CAACACAAAC TGCCTCCTCC
    ATGGACAGCG CTCATTCAGA CGTGGCATCA GGACTGAGTG ACGGAAACGA GGGCAGCACA
    GGTGGGAGAC ACGAAGGCCG AGCCACTAAA CGACACTACC GAAAATCTGT GAGAAGCCGC
    TCCAGGCACG AGAAAACTGC ACGCCCAAAG CTTCGAATCC TTAATGTTTC CAACAAAGGG
    GATCGTGTTG TAGAGTGTCA GCTGGAGACT CACAACCGCA AGATGGTCAC CTTCAAATTC
    GACCTGGATG GAGACAATCC AGAGGAGATT GCTACTATAA TGGTACAGAA TGTGTTTATT
    TTGGCTACGG AGAGAGAATC CTTTGTAGAG CAGGTAAAAG ACATAATTGA GAAGGCCGAT
    GAAATGTTGA GCGAAGATGT GAGCGTGGAG CCGGAAGGAG AAACGAGGGC AGAAAGTTTG
    CAAATCAAGG ATGATGGGGA TTACCCAGAA TCTCAGAAGA TGAATTCTGA ATTTCGAATG
    CCAGATCCTG TATATTCCCT ACCACAGACA GCAGGGCTTA TTCCCAGCGT TGTCACACAA
    GTCGTTCATT CTGCCGGAAG ACGATTTATT GTCAGTCCGG TGCCTGAAAG CCGTCTAAAA
    GAACAGTCAT TCTTCAGTAC TTCATTGCAG GACCCTCAGC TACCAGTCTC TGTCGCGCAT
    GGTCCAGGGA TGAATTTGTC TCATTCTGCC TCTTCTGTGA GCCTGCAGCA AGCCTTTGCA
    GAACTACGCC AGTCACAGAT GAGCGAGGGT CCTAGCACAG CTCCTCCCAT ATTTAACCAA
    ACTGGACCCC CCTTTCCACC ATCCATGACT AGTCTGGTGG CAACTCTGCC TTCTAATGCA
    ATGCCTATCC TCCAGGCAGC CCCAGTGATC CCCAGTAGCC TGCCATCTTC TGTGCCTCAG
    CCCCTGGTGG AGGTGGTTGT ACCTCAGGCT GCTGGGATGG CTCCAACTAG CAATGTTCCA
    AGCACCTTGT CCCCTCCCCT AACTGCAGTA TCCAACCAGC ACGCAGACAT GATACCGGGT
    GCTGCTGTAG CAGCTATCGG ATCACTTCCT GTGACATCAC TTCCTCCTTT GCCCATTGTG
    GGTGGAGTTC CTGTAGGCAC TGCAACCCTG AGCTCACCCC TAACACCCCT AATCAGTTCT
    GTCAGTACAA TGGGGGGGAC GGTTCCCCCA TCTGTCACAT CTCCCCCAGT GCCACAGATA
    GCGGGTAGCG TTGCCGGGAG CCTGCCTCCT GCATCAGCCT CCGCCCCTCT TATTCATGGC
    AGTGTTGCCC CATCTCAGGT ACCGCTTTGC AGCAGCAGTT CTGTTCCCAG CTTTGCAGAG
    AGCGTGGCAG TCAGCCCCCT GCTGGATAGT GGCCAAAAGG CAGCAGAAGT GGGCACGGTA
    ATGAGCACGA CTGGCTTGTC TCTGCCCATC TCTGCACCTC TGACATATGC TGGGGTGGTT
    GCAGCATCAG GCATCACAAG TCAACCTGCC GTGCATCTCC CCCAGGGGGT GGCCACAACA
    CCAGTTGTAT CCCAGGCAGT AGGGGGAGCT GTGGGCACAC CCCTCTCATC TCTTCCTCCC
    ATCCCTGGAG GCATGTCTGT TAGCCAACCA GGATCAAATA TACCCCCTGT GCAACACACG
    TTGGCCCATG GCCAACCCCA GCCTACCCCT CTCCCCAACC AGCCTCATAC GCACCTTGTA
    GAGGGTGACA CCGATAACCT GCCCAAACCT GGCATAGACG ATATAAAAAC CCTGGAGGAG
    AAGCTGAGGT CGCTGTTCAG TGAGCATGGC AGTGCCGGGG CCCCTCATGG ACTCACCCCA
    TCAGAAACTC AATTGGGTTT GGATGGGGCC CCCCTGCCAG GCCCTTCCAC AGGTAGCACC
    GCTCAGACCA AGCCTGCAGC ATCCTCTTCT CCACCAACCA GCTTACCTTT AGGGCCACCT
    GGAGTGACGA CAATGACTCA GATCATGACA CCTGGACAAG CTGCTACACC TGTCACCTAC
    GTATCTGCAG CTGGAAGCTC TATAGCCACG GCCAAAGCTG CCACCCCACC CTCAAAGCCG
    CCTCTCAGTC GGGTACCGGT GACCACGGCC GGGGCAGAGA CCCCAGCAGG CAGTCTCCCT
    GGTGAACACC TGCCTCCTCC TTCTCTCCCA GGACCTTCAC TAACTCAGTC CCAGCAGCCT
    CTAGAAGACC TGGATTTCCA GCTGAGAAGG ACGTTGAGCC CTGACACTGT CCCTGTGCCT
    TCTGCACTAA TATTTCCTGC ACACAGCGGA GCTTCTACCG GTGTTGCAGC ACCCGTCGGC
    TCAGCATTGC AACCCTCCGC TTCAGAATTG CCAAAAGCCA GCGCTGGAGA GGCAGAGGTG
    GGCGCGAGTG TCCAGAGGAT GGGCAGGTTT CAGGTCTCTT TGGCAGCAGA GAATGTGCTC
    AAGCAGAGCG AGGAGACCAA ACTGTCCATC TCAGACACTT CCTCCACCAC TTCTACATCC
    TCCTCTTCCT CTGCGTCATG CAGCAGCCCA GAGAGCACCC TGACTAAGCC CCAGGCCAAA
    ACCGCTGCTC CCTTAGACAT AGTGGATGGT GTTGGTCCCC ACTTGGTGCA TCTACAGAGC
    CTATCTCCCA AGCAGTTGGT GAAAGTGGGG CGCTTCCAGG TTACCACTGC CACCACGGAC
    AAAGTGGGGC GCTTTTCTGT GTCCCGGACA CAAGATGATA TATCTGCTGG GGTGCTGGAG
    ACAGACTCCC AGAGGTCCTC CTCCCCACAG ACTGAGAAAC ATCTGAATGG ACCCTACTTA
    CAGCAAGACT CCTCTGCGGC TCTCGGCTAT CCGGCCAGGG AGACGCAGGA AGACAGCCTA
    GGGAGCCCCG TTCTGCACCA CGCTTTGGGG TCCAAGCTTA GCCTGCAGAG CCTTAGCAAC
    TCGTTCAACT CTTCCTACAT GAGCAGCGAT AATGATTCTG ACATAGAAGA TGAAGATCTG
    AAGAAGGAGC TGCGAAGGCT ACGAGACAAG CATCTCAAGG AGATTCAGGA ACTGCAGAGT
    CGACAGAAGC AGGAGATCGA GCTGTTGTAC ACGGCTTTGG GAAAAGTGCC CCCTGCTGTC
    ATTATCCCTC CGGCAGCGCC CCTTTCTGGC CGGCGCAGGA GGCATACCAA GGGGAAGGGC
    AGCAAGTCCA GCCGGGGCAG CTCCCACAAC AGCCGCAGCC CTCATTTGTC AGGCGACCTG
    TCCACGCAGA GCGCTCCGTC GGCATTACCC CCCCAGCCGG GCCTTCTGCT CCCCCCAAGC
    AGTGCGCCCG AACATGCTCA TCTTCTCAAG CCCTCCCCTT CCAGCGACAT TCTCTGCTCA
    GCCTTCACTA GCGACGCTGC CCTCTCTGTG CCCAGCCTGT CCACCCAGGG CCAAGGCTGG
    GCCAAGTTTA ACTGTGAGTC AGAGCGGGTA TCCTTCAAAA TGGCTGGCAG GAGGACGCGA
    TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA GGACCAGCAG CACTAACACA GTCGCACCAT CAACTGCCCC GGCCCCCCAG
    CCAAACGTGC CCCCCGTGTC ATCCAGCCGC AAGGGAACCT TCACGGACGA TCTACACAAA
    CTGGTGGACA ACTGGGCCCG AGACGCCATG AACCTGACTG GAAAAAAGGC TTGTAAGGCC
    TCTGGGCAAC CCAGCTATGA GGGACCCGGG ATGGCCCGTA AGTTCTCTGC TCCCGGACAG
    CTGTGTGTCC CCATGAACCC ATCCCTGGGG GGCTCCGTCC CTCTCCCAGC AGCTTCGGCG
    ACCTCCCTGG CTCACTACCC CAAAGGAATG TGCCCCCCGC AACAATACGG AATCCCCGGG
    GCGCTGTTCC AAGCTCCATG GAGTGGCACC GGCGTGCCCC CCTCCTCTCA GCCCCTAAGC
    CAGTTCCCCC CGGCGGTGAC ATCTTCCTTA CAGAACTTCG GTATTAGCAG CTTGCAGAAA
    TCTATCAGCA ACCCCCAAGG ATCCAACCTG CGGACTACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54270
    Clone ID Related Accession (Same CDS sequence) XM_031898629.1
    Accession Version XM_031898629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCA CAAATATCAT CTGGACAACC ACCCACCACA
    TCCATGGGAA TCAGTGCTCA GCCCGGTCCC CACACCCAGG GCATGGTGCA TCATCAGCCT
    TCTCTGCATC CAGCCTCGCA GTACCAAGTG CAGCAGCCAA TGCCTACTGT GGGTTCCAGT
    ATTGCGGCCC CATCACAGCC GGTGATTACC CAAACGCCAG CCCCTCAGCC GGGCACCCAG
    CTCCCTGCCT CCCAGCCTTT GCCTGCTGTT CAAGGAGAGC CTCAGATTCC TGCTCAGACA
    GCTCCTGCTC CCCAGCCCGT TCCTACAGTG CCGGCCTCTC TGCCACCTCA TTTTCTTCCC
    TTGGCACAAT CTTTGGTTGC AGTCCCTCTC ATTCCTCCAT TCGCTGCCCC ACAGTTGCCT
    TTAACGGTAC CACCCCCGGT CATACAGACA GGGTTCCCTT CCCTACCCAT GACTGTAACC
    CCAAGTATTG GCCAAACTGT ACCAGGAATA CCCCCATCCT TAGGAGCGCC AGTCCTGCCC
    CATGGGCAGG CAGTGTTAGT TCCCACTCAC ATCACTAATG TTGCTCTCCA GCCTTTGGCA
    CCAATCCCCA CACCTCCTCT GTGCCCTGTA ACTCAGCCCA TTGGATTACC GGCCACCCTG
    GGAAAAGCTG CTGAGCCCTT GCTTCCTCCG GGAGATACTT CTTACCAGGT TTTCCAGGCT
    ACTCACCCTC TATTCCCTGG AGATGTAAGC TTTGTTCCTC CCGTTGCCAT CCCCTCAGCA
    ATGGTATCTC CCCCTATCCG TGGTGAAAGT TTAGCCCAAC CTGGCTTCCA TACTCAGCCT
    TATCCTGAAA ACAGCAATTT GTATTCCCAT GGAGGGCCCA CTCCCCAGCC CGCTGTTCTC
    ACTACCCAGC CGGTGCCTGC AGGAACCCAG ACGCAACCTG CCGTCGAGGG TTCACAGAGT
    AGCTCCCTGG CAATTTCCCA GGATACCCAG TCATCTCCTC AAGCAACACA AACTGCCTCC
    TCCATGGACA GCGCTCATTC AGACGTGGCA TCAGGACTGA GTGACGGAAA CGAGGGCAGC
    ACAGGTGGGA GACACGAAGG CCGAGCCACT AAACGACACT ACCGAAAATC TGTGAGAAGC
    CGCTCCAGGC ACGAGAAAAC TGCACGCCCA AAGCTTCGAA TCCTTAATGT TTCCAACAAA
    GGGGATCGTG TTGTAGAGTG TCAGCTGGAG ACTCACAACC GCAAGATGGT CACCTTCAAA
    TTCGACCTGG ATGGAGACAA TCCAGAGGAG ATTGCTACTA TAATGGTACA GAATGTGTTT
    ATTTTGGCTA CGGAGAGAGA ATCCTTTGTA GAGCAGGTAA AAGACATAAT TGAGAAGGCC
    GATGAAATGT TGAGCGAAGA TGTGAGCGTG GAGCCGGAAG GAGAAACGAG GGCAGAAAGT
    TTGCAAATCA AGGATGATGG GGATTACCCA GAATCTCAGA AGATGAATTC TGAATTTCGA
    ATGCCAGATC CTGTATATTC CCTACCACAG ACAGCAGGGC TTATTCCCAG CGTTGTCACA
    CAAGTCGTTC ATTCTGCCGG AAGACGATTT ATTGTCAGTC CGGTGCCTGA AAGCCGTCTA
    AAAGAACAGT CATTCTTCAG TACTTCATTG CAGGACCCTC AGCTACCAGT CTCTGTCGCG
    CATGGTCCAG GGATGAATTT GTCTCATTCT GCCTCTTCTG TGAGCCTGCA GCAAGCCTTT
    GCAGAACTAC GCCAGTCACA GATGAGCGAG GGTCCTAGCA CAGCTCCTCC CATATTTAAC
    CAAACTGGAC CCCCCTTTCC ACCATCCATG ACTAGTCTGG TGGCAACTCT GCCTTCTAAT
    GCAATGCCTA TCCTCCAGGC AGCCCCAGTG ATCCCCAGTA GCCTGCCATC TTCTGTGCCT
    CAGCCCCTGG TGGAGGTGGT TGTACCTCAG GCTGCTGGGA TGGCTCCAAC TAGCAATGTT
    CCAAGCACCT TGTCCCCTCC CCTAACTGCA GTATCCAACC AGCACGCAGA CATGATACCG
    GGTGCTGCTG TAGCAGCTAT CGGATCACTT CCTGTGACAT CACTTCCTCC TTTGCCCATT
    GTGGGTGGAG TTCCTGTAGG CACTGCAACC CTGAGCTCAC CCCTAACACC CCTAATCAGT
    TCTGTCAGTA CAATGGGGGG GACGGTTCCC CCATCTGTCA CATCTCCCCC AGTGCCACAG
    ATAGCGGGTA GCGTTGCCGG GAGCCTGCCT CCTGCATCAG CCTCCGCCCC TCTTATTCAT
    GGCAGTGTTG CCCCATCTCA GGTACCGCTT TGCAGCAGCA GTTCTGTTCC CAGCTTTGCA
    GAGAGCGTGG CAGTCAGCCC CCTGCTGGAT AGTGGCCAAA AGGCAGCAGA AGTGGGCACG
    GTAATGAGCA CGACTGGCTT GTCTCTGCCC ATCTCTGCAC CTCTGACATA TGCTGGGGTG
    GTTGCAGCAT CAGGCATCAC AAGTCAACCT GCCGTGCATC TCCCCCAGGG GGTGGCCACA
    ACACCAGTTG TATCCCAGGC AGTAGGGGGA GCTGTGGGCA CACCCCTCTC ATCTCTTCCT
    CCCATCCCTG GAGGCATGTC TGTTAGCCAA CCAGGATCAA ATATACCCCC TGTGCAACAC
    ACGTTGGCCC ATGGCCAACC CCAGCCTACC CCTCTCCCCA ACCAGCCTCA TACGCACCTT
    GTAGAGGGTG ACACCGATAA CCTGCCCAAA CCTGGCATAG ACGATATAAA AACCCTGGAG
    GAGAAGCTGA GGTCGCTGTT CAGTGAGCAT GGCAGTGCCG GGGCCCCTCA TGGACTCACC
    CCATCAGAAA CTCAATTGGG TTTGGATGGG GCCCCCCTGC CAGGCCCTTC CACAGGTAGC
    ACCGCTCAGA CCAAGCCTGC AGCATCCTCT TCTCCACCAA CCAGCTTACC TTTAGGGCCA
    CCTGGAGTGA CGACAATGAC TCAGATCATG ACACCTGGAC AAGCTGCTAC ACCTGTCACC
    TACGTATCTG CAGCTGGAAG CTCTATAGCC ACGGCCAAAG CTGCCACCCC ACCCTCAAAG
    CCGCCTCTCA GTCGGGTACC GGTGACCACG GCCGGGGCAG AGACCCCAGC AGGCAGTCTC
    CCTGGTGAAC ACCTGCCTCC TCCTTCTCTC CCAGGACCTT CACTAACTCA GTCCCAGCAG
    CCTCTAGAAG ACCTGGATTT CCAGCTGAGA AGGACGTTGA GCCCTGACAC TGTCCCTGTG
    CCTTCTGCAC TAATATTTCC TGCACACAGC GGAGCTTCTA CCGGTGTTGC AGCACCCGTC
    GGCTCAGCAT TGCAACCCTC CGCTTCAGAA TTGCCAAAAG CCAGCGCTGG AGAGGCAGAG
    GTGGGCGCGA GTGTCCAGAG GATGGGCAGG TTTCAGGTCT CTTTGGCAGC AGAGAATGTG
    CTCAAGCAGA GCGAGGAGAC CAAACTGTCC ATCTCAGACA CTTCCTCCAC CACTTCTACA
    TCCTCCTCTT CCTCTGCGTC ATGCAGCAGC CCAGAGAGCA CCCTGACTAA GCCCCAGGCC
    AAAACCGCTG CTCCCTTAGA CATAGTGGAT GGTGTTGGTC CCCACTTGGT GCATCTACAG
    AGCCTATCTC CCAAGCAGTT GGTGAAAGTG GGGCGCTTCC AGGTTACCAC TGCCACCACG
    GACAAAGTGG GGCGCTTTTC TGTGTCCCGG ACACAAGATG ATATATCTGC TGGGGTGCTG
    GAGACAGACT CCCAGAGGTC CTCCTCCCCA CAGACTGAGA AACATCTGAA TGGACCCTAC
    TTACAGCAAG ACTCCTCTGC GGCTCTCGGC TATCCGGCCA GGGAGACGCA GGAAGACAGC
    CTAGGGAGCC CCGTTCTGCA CCACGCTTTG GGGTCCAAGC TTAGCCTGCA GAGCCTTAGC
    AACTCGTTCA ACTCTTCCTA CATGAGCAGC GATAATGATT CTGACATAGA AGATGAAGAT
    CTGAAGAAGG AGCTGCGAAG GCTACGAGAC AAGCATCTCA AGGAGATTCA GGAACTGCAG
    AGTCGACAGA AGCAGGAGAT CGAGCTGTTG TACACGGCTT TGGGAAAAGT GCCCCCTGCT
    GTCATTATCC CTCCGGCAGC GCCCCTTTCT GGCCGGCGCA GGAGGCATAC CAAGGGGAAG
    GGCAGCAAGT CCAGCCGGGG CAGCTCCCAC AACAGCCGCA GCCCTCATTT GTCAGGCGAC
    CTGTCCACGC AGAGCGCTCC GTCGGCATTA CCCCCCCAGC CGGGCCTTCT GCTCCCCCCA
    AGCAGTGCGC CCGAACATGC TCATCTTCTC AAGCCCTCCC CTTCCAGCGA CATTCTCTGC
    TCAGCCTTCA CTAGCGACGC TGCCCTCTCT GTGCCCAGCC TGTCCACCCA GGGCCAAGGC
    TGGGCCAAGT TTAACTGTGA GTCAGAGCGG GTATCCTTCA AAATGGCTGG CAGGAGGACG
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTAGGACCAG CAGCACTAAC ACAGTCGCAC CATCAACTGC CCCGGCCCCC
    CAGCCAAACG TGCCCCCCGT GTCATCCAGC CGCAAGGGAA CCTTCACGGA CGATCTACAC
    AAACTGGTGG ACAACTGGGC CCGAGACGCC ATGAACCTGA CTGGAAAAAA GGCTTGTAAG
    GCCTCTGGGC AACCCAGCTA TGAGGGACCC GGGATGGCCC GTAAGTTCTC TGCTCCCGGA
    CAGCTGTGTG TCCCCATGAA CCCATCCCTG GGGGGCTCCG TCCCTCTCCC AGCAGCTTCG
    GCGACCTCCC TGGCTCACTA CCCCAAAGGA ATGTGCCCCC CGCAACAATA CGGAATCCCC
    GGGGCGCTGT TCCAAGCTCC ATGGAGTGGC ACCGGCGTGC CCCCCTCCTC TCAGCCCCTA
    AGCCAGTTCC CCCCGGCGGT GACATCTTCC TTACAGAACT TCGGTATTAG CAGCTTGCAG
    AAATCTATCA GCAACCCCCA AGGATCCAAC CTGCGGACTA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754489.1
    CDS324..7328
    Translation

    Target ORF information:

    RefSeq Version XM_031898629.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCA CAAATATCAT CTGGACAACC ACCCACCACA
    TCCATGGGAA TCAGTGCTCA GCCCGGTCCC CACACCCAGG GCATGGTGCA TCATCAGCCT
    TCTCTGCATC CAGCCTCGCA GTACCAAGTG CAGCAGCCAA TGCCTACTGT GGGTTCCAGT
    ATTGCGGCCC CATCACAGCC GGTGATTACC CAAACGCCAG CCCCTCAGCC GGGCACCCAG
    CTCCCTGCCT CCCAGCCTTT GCCTGCTGTT CAAGGAGAGC CTCAGATTCC TGCTCAGACA
    GCTCCTGCTC CCCAGCCCGT TCCTACAGTG CCGGCCTCTC TGCCACCTCA TTTTCTTCCC
    TTGGCACAAT CTTTGGTTGC AGTCCCTCTC ATTCCTCCAT TCGCTGCCCC ACAGTTGCCT
    TTAACGGTAC CACCCCCGGT CATACAGACA GGGTTCCCTT CCCTACCCAT GACTGTAACC
    CCAAGTATTG GCCAAACTGT ACCAGGAATA CCCCCATCCT TAGGAGCGCC AGTCCTGCCC
    CATGGGCAGG CAGTGTTAGT TCCCACTCAC ATCACTAATG TTGCTCTCCA GCCTTTGGCA
    CCAATCCCCA CACCTCCTCT GTGCCCTGTA ACTCAGCCCA TTGGATTACC GGCCACCCTG
    GGAAAAGCTG CTGAGCCCTT GCTTCCTCCG GGAGATACTT CTTACCAGGT TTTCCAGGCT
    ACTCACCCTC TATTCCCTGG AGATGTAAGC TTTGTTCCTC CCGTTGCCAT CCCCTCAGCA
    ATGGTATCTC CCCCTATCCG TGGTGAAAGT TTAGCCCAAC CTGGCTTCCA TACTCAGCCT
    TATCCTGAAA ACAGCAATTT GTATTCCCAT GGAGGGCCCA CTCCCCAGCC CGCTGTTCTC
    ACTACCCAGC CGGTGCCTGC AGGAACCCAG ACGCAACCTG CCGTCGAGGG TTCACAGAGT
    AGCTCCCTGG CAATTTCCCA GGATACCCAG TCATCTCCTC AAGCAACACA AACTGCCTCC
    TCCATGGACA GCGCTCATTC AGACGTGGCA TCAGGACTGA GTGACGGAAA CGAGGGCAGC
    ACAGGTGGGA GACACGAAGG CCGAGCCACT AAACGACACT ACCGAAAATC TGTGAGAAGC
    CGCTCCAGGC ACGAGAAAAC TGCACGCCCA AAGCTTCGAA TCCTTAATGT TTCCAACAAA
    GGGGATCGTG TTGTAGAGTG TCAGCTGGAG ACTCACAACC GCAAGATGGT CACCTTCAAA
    TTCGACCTGG ATGGAGACAA TCCAGAGGAG ATTGCTACTA TAATGGTACA GAATGTGTTT
    ATTTTGGCTA CGGAGAGAGA ATCCTTTGTA GAGCAGGTAA AAGACATAAT TGAGAAGGCC
    GATGAAATGT TGAGCGAAGA TGTGAGCGTG GAGCCGGAAG GAGAAACGAG GGCAGAAAGT
    TTGCAAATCA AGGATGATGG GGATTACCCA GAATCTCAGA AGATGAATTC TGAATTTCGA
    ATGCCAGATC CTGTATATTC CCTACCACAG ACAGCAGGGC TTATTCCCAG CGTTGTCACA
    CAAGTCGTTC ATTCTGCCGG AAGACGATTT ATTGTCAGTC CGGTGCCTGA AAGCCGTCTA
    AAAGAACAGT CATTCTTCAG TACTTCATTG CAGGACCCTC AGCTACCAGT CTCTGTCGCG
    CATGGTCCAG GGATGAATTT GTCTCATTCT GCCTCTTCTG TGAGCCTGCA GCAAGCCTTT
    GCAGAACTAC GCCAGTCACA GATGAGCGAG GGTCCTAGCA CAGCTCCTCC CATATTTAAC
    CAAACTGGAC CCCCCTTTCC ACCATCCATG ACTAGTCTGG TGGCAACTCT GCCTTCTAAT
    GCAATGCCTA TCCTCCAGGC AGCCCCAGTG ATCCCCAGTA GCCTGCCATC TTCTGTGCCT
    CAGCCCCTGG TGGAGGTGGT TGTACCTCAG GCTGCTGGGA TGGCTCCAAC TAGCAATGTT
    CCAAGCACCT TGTCCCCTCC CCTAACTGCA GTATCCAACC AGCACGCAGA CATGATACCG
    GGTGCTGCTG TAGCAGCTAT CGGATCACTT CCTGTGACAT CACTTCCTCC TTTGCCCATT
    GTGGGTGGAG TTCCTGTAGG CACTGCAACC CTGAGCTCAC CCCTAACACC CCTAATCAGT
    TCTGTCAGTA CAATGGGGGG GACGGTTCCC CCATCTGTCA CATCTCCCCC AGTGCCACAG
    ATAGCGGGTA GCGTTGCCGG GAGCCTGCCT CCTGCATCAG CCTCCGCCCC TCTTATTCAT
    GGCAGTGTTG CCCCATCTCA GGTACCGCTT TGCAGCAGCA GTTCTGTTCC CAGCTTTGCA
    GAGAGCGTGG CAGTCAGCCC CCTGCTGGAT AGTGGCCAAA AGGCAGCAGA AGTGGGCACG
    GTAATGAGCA CGACTGGCTT GTCTCTGCCC ATCTCTGCAC CTCTGACATA TGCTGGGGTG
    GTTGCAGCAT CAGGCATCAC AAGTCAACCT GCCGTGCATC TCCCCCAGGG GGTGGCCACA
    ACACCAGTTG TATCCCAGGC AGTAGGGGGA GCTGTGGGCA CACCCCTCTC ATCTCTTCCT
    CCCATCCCTG GAGGCATGTC TGTTAGCCAA CCAGGATCAA ATATACCCCC TGTGCAACAC
    ACGTTGGCCC ATGGCCAACC CCAGCCTACC CCTCTCCCCA ACCAGCCTCA TACGCACCTT
    GTAGAGGGTG ACACCGATAA CCTGCCCAAA CCTGGCATAG ACGATATAAA AACCCTGGAG
    GAGAAGCTGA GGTCGCTGTT CAGTGAGCAT GGCAGTGCCG GGGCCCCTCA TGGACTCACC
    CCATCAGAAA CTCAATTGGG TTTGGATGGG GCCCCCCTGC CAGGCCCTTC CACAGGTAGC
    ACCGCTCAGA CCAAGCCTGC AGCATCCTCT TCTCCACCAA CCAGCTTACC TTTAGGGCCA
    CCTGGAGTGA CGACAATGAC TCAGATCATG ACACCTGGAC AAGCTGCTAC ACCTGTCACC
    TACGTATCTG CAGCTGGAAG CTCTATAGCC ACGGCCAAAG CTGCCACCCC ACCCTCAAAG
    CCGCCTCTCA GTCGGGTACC GGTGACCACG GCCGGGGCAG AGACCCCAGC AGGCAGTCTC
    CCTGGTGAAC ACCTGCCTCC TCCTTCTCTC CCAGGACCTT CACTAACTCA GTCCCAGCAG
    CCTCTAGAAG ACCTGGATTT CCAGCTGAGA AGGACGTTGA GCCCTGACAC TGTCCCTGTG
    CCTTCTGCAC TAATATTTCC TGCACACAGC GGAGCTTCTA CCGGTGTTGC AGCACCCGTC
    GGCTCAGCAT TGCAACCCTC CGCTTCAGAA TTGCCAAAAG CCAGCGCTGG AGAGGCAGAG
    GTGGGCGCGA GTGTCCAGAG GATGGGCAGG TTTCAGGTCT CTTTGGCAGC AGAGAATGTG
    CTCAAGCAGA GCGAGGAGAC CAAACTGTCC ATCTCAGACA CTTCCTCCAC CACTTCTACA
    TCCTCCTCTT CCTCTGCGTC ATGCAGCAGC CCAGAGAGCA CCCTGACTAA GCCCCAGGCC
    AAAACCGCTG CTCCCTTAGA CATAGTGGAT GGTGTTGGTC CCCACTTGGT GCATCTACAG
    AGCCTATCTC CCAAGCAGTT GGTGAAAGTG GGGCGCTTCC AGGTTACCAC TGCCACCACG
    GACAAAGTGG GGCGCTTTTC TGTGTCCCGG ACACAAGATG ATATATCTGC TGGGGTGCTG
    GAGACAGACT CCCAGAGGTC CTCCTCCCCA CAGACTGAGA AACATCTGAA TGGACCCTAC
    TTACAGCAAG ACTCCTCTGC GGCTCTCGGC TATCCGGCCA GGGAGACGCA GGAAGACAGC
    CTAGGGAGCC CCGTTCTGCA CCACGCTTTG GGGTCCAAGC TTAGCCTGCA GAGCCTTAGC
    AACTCGTTCA ACTCTTCCTA CATGAGCAGC GATAATGATT CTGACATAGA AGATGAAGAT
    CTGAAGAAGG AGCTGCGAAG GCTACGAGAC AAGCATCTCA AGGAGATTCA GGAACTGCAG
    AGTCGACAGA AGCAGGAGAT CGAGCTGTTG TACACGGCTT TGGGAAAAGT GCCCCCTGCT
    GTCATTATCC CTCCGGCAGC GCCCCTTTCT GGCCGGCGCA GGAGGCATAC CAAGGGGAAG
    GGCAGCAAGT CCAGCCGGGG CAGCTCCCAC AACAGCCGCA GCCCTCATTT GTCAGGCGAC
    CTGTCCACGC AGAGCGCTCC GTCGGCATTA CCCCCCCAGC CGGGCCTTCT GCTCCCCCCA
    AGCAGTGCGC CCGAACATGC TCATCTTCTC AAGCCCTCCC CTTCCAGCGA CATTCTCTGC
    TCAGCCTTCA CTAGCGACGC TGCCCTCTCT GTGCCCAGCC TGTCCACCCA GGGCCAAGGC
    TGGGCCAAGT TTAACTGTGA GTCAGAGCGG GTATCCTTCA AAATGGCTGG CAGGAGGACG
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTAGGACCAG CAGCACTAAC ACAGTCGCAC CATCAACTGC CCCGGCCCCC
    CAGCCAAACG TGCCCCCCGT GTCATCCAGC CGCAAGGGAA CCTTCACGGA CGATCTACAC
    AAACTGGTGG ACAACTGGGC CCGAGACGCC ATGAACCTGA CTGGAAAAAA GGCTTGTAAG
    GCCTCTGGGC AACCCAGCTA TGAGGGACCC GGGATGGCCC GTAAGTTCTC TGCTCCCGGA
    CAGCTGTGTG TCCCCATGAA CCCATCCCTG GGGGGCTCCG TCCCTCTCCC AGCAGCTTCG
    GCGACCTCCC TGGCTCACTA CCCCAAAGGA ATGTGCCCCC CGCAACAATA CGGAATCCCC
    GGGGCGCTGT TCCAAGCTCC ATGGAGTGGC ACCGGCGTGC CCCCCTCCTC TCAGCCCCTA
    AGCCAGTTCC CCCCGGCGGT GACATCTTCC TTACAGAACT TCGGTATTAG CAGCTTGCAG
    AAATCTATCA GCAACCCCCA AGGATCCAAC CTGCGGACTA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54272
    Clone ID Related Accession (Same CDS sequence) XM_031898632.1
    Accession Version XM_031898632.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCATCGTCC ATGGTCCATT CAGCACTCCC AGACTCTCCG
    CTTTTTTTCC CAACCATTCC CAACCAGCGC CCCATGTCTT TCTCTCCGCC GCCTACTGGC
    TTGTCCCCAA AGGTGCCTGC AACCCAGCGA CGCAAGAGCA CCTCTTTCTT GGAGTCCCAA
    GGCCGCCAGG GGCTATCTCA GATTAGGACA CTCGGGCCAG GTGAGGAGAC CCAGTGCTGG
    AGGGCAGACT ACATGGCAGC TCTAGGTAAA GCATCTGTCC GATCCGAGGA TGCTCACAAG
    CATCAAAACT ATGAGGGATT GGCGTTCCAT GACGCAAGAC ATGTTCCGAC AGTTCCAAAT
    GAAACTCTCT TAAAGCCTGG GGAAGCATTG TATCTAGCCA GTCTGCCCTC ACAGGAGAGC
    TACAGCAGCA TGGCTGCCCC TGAGCATATG GAGGGCCACA AGCTGACCCA GCTTGGCTCG
    TTTTACGATT ATCACACTGG GCAAACCTAC ATTGCGCGGC CCCTGAAGAG CCTAAGACTG
    GAATCTGGGT TAAACCAGCC GTCGGCTTTG GGTAGCATCT CCAACCCAAT CTCCTCCGAC
    CCACACGCCA TGTTTCATCA GATGTTTGTG CCCCAGTCTG CTCCTCCAGT GCTGGCTCAA
    AGCAGCGAAG GCCACCCAGG GTGTGTGTTT GAGTTCCACG TGCACCCATC TGGGTCTGTT
    CCCGGCGAAG CAACCGTTTA CTTGCCACAC CGAGTTTACC GTCCTCGACG GGGCTCTGTA
    GACTTTAACC CAGAAGAACA GCCGCCGGGT TCTACGCTGC AGAGCCGCCT CCAAACTGTG
    ACTGAAGAGC AGTGCCACCA CAATGGCCCA GAACCACTCC CACCCTTTAT AGGATTCATC
    ACATGCAACC GTATGGAGAG CAGCCCCGAC TATTCCAGTG ATTCTTCCCA AAGGAATTCA
    TCCGATCCCG ATTATCTCTC TCCTCCTCTG GCCTTCAGCT CCAATATCTC TATGGCTTCC
    AAGCAGAAAA CGTTGCAGGA TCCACAGATT TTCTTTTGTG TCCCACAGTC CGGAACGACG
    GCTTATGCTG GACCATCAGC TTTGTACAGC CAGCAGCCAC AAATATCATC TGGACAACCA
    CCCACCACAT CCATGGGAAT CAGTGCTCAG CCCGGTCCCC ACACCCAGGG CATGGTGCAT
    CATCAGCCTT CTCTGCATCC AGCCTCGCAG TACCAAGTGC AGCAGCCAAT GCCTACTGTG
    GGTTCCAGTA TTGCGGCCCC ATCACAGCCG GTGATTACCC AAACGCCAGC CCCTCAGCCG
    GGCACCCAGC TCCCTGCCTC CCAGCCTTTG CCTGCTGTTC AAGGAGAGCC TCAGATTCCT
    GCTCAGACAG CTCCTGCTCC CCAGCCCGTT CCTACAGTGC CGGCCTCTCT GCCACCTCAT
    TTTCTTCCCT TGGCACAATC TTTGGTTGCA GTCCCTCTCA TTCCTCCATT CGCTGCCCCA
    CAGTTGCCTT TAACGGTACC ACCCCCGGTC ATACAGACAG GGTTCCCTTC CCTACCCATG
    ACTGTAACCC CAAGTATTGG CCAAACTGTA CCAGGAATAC CCCCATCCTT AGGAGCGCCA
    GTCCTGCCCC ATGGGCAGGC AGTGTTAGTT CCCACTCACA TCACTAATGT TGCTCTCCAG
    CCTTTGGCAC CAATCCCCAC ACCTCCTCTG TGCCCTGTAA CTCAGCCCAT TGGATTACCG
    GCCACCCTGG GAAAAGCTGC TGAGCCCTTG CTTCCTCCGG GAGATACTTC TTACCAGGTT
    TTCCAGGCTA CTCACCCTCT ATTCCCTGGA GATGTAAGCT TTGTTCCTCC CGTTGCCATC
    CCCTCAGCAA TGGTATCTCC CCCTATCCGT GGTGAAAGTT TAGCCCAACC TGGCTTCCAT
    ACTCAGCCTT ATCCTGAAAA CAGCAATTTG TATTCCCATG GAGGGCCCAC TCCCCAGCCC
    GCTGTTCTCA CTACCCAGCC GGTGCCTGCA GGAACCCAGA CGCAACCTGC CGTCGAGGGT
    TCACAGAGTA GCTCCCTGGC AATTTCCCAG GATACCCAGT CATCTCCTCA AGCAACACAA
    ACTGCCTCCT CCATGGACAG CGCTCATTCA GACGTGGCAT CAGGACTGAG TGACGGAAAC
    GAGGGCAGCA CAGGTGGGAG ACACGAAGGC CGAGCCACTA AACGACACTA CCGAAAATCT
    GTGAGAAGCC GCTCCAGGCA CGAGAAAACT GCACGCCCAA AGCTTCGAAT CCTTAATGTT
    TCCAACAAAG GGGATCGTGT TGTAGAGTGT CAGCTGGAGA CTCACAACCG CAAGATGGTC
    ACCTTCAAAT TCGACCTGGA TGGAGACAAT CCAGAGGAGA TTGCTACTAT AATGGTACAG
    AATGTGTTTA TTTTGGCTAC GGAGAGAGAA TCCTTTGTAG AGCAGGTAAA AGACATAATT
    GAGAAGGCCG ATGAAATGTT GAGCGAAGAT GTGAGCGTGG AGCCGGAAGG AGAAACGAGG
    GCAGAAAGTT TGCAAATCAA GGATGATGGG GATTACCCAG AATCTCAGAA GATGAATTCT
    GAATTTCGAA TGCCAGATCC TGTATATTCC CTACCACAGA CAGCAGGGCT TATTCCCAGC
    GTTGTCACAC AAGTCGTTCA TTCTGCCGGA AGACGATTTA TTGTCAGTCC GGTGCCTGAA
    AGCCGTCTAA AAGAACAGTC ATTCTTCAGT ACTTCATTGC AGGACCCTCA GCTACCAGTC
    TCTGTCGCGC ATGGTCCAGG GATGAATTTG TCTCATTCTG CCTCTTCTGT GAGCCTGCAG
    CAAGCCTTTG CAGAACTACG CCAGTCACAG ATGAGCGAGG GTCCTAGCAC AGCTCCTCCC
    ATATTTAACC AAACTGGACC CCCCTTTCCA CCATCCATGA CTAGTCTGGT GGCAACTCTG
    CCTTCTAATG CAATGCCTAT CCTCCAGGCA GCCCCAGTGA TCCCCAGTAG CCTGCCATCT
    TCTGTGCCTC AGCCCCTGGT GGAGGTGGTT GTACCTCAGG CTGCTGGGAT GGCTCCAACT
    AGCAATGTTC CAAGCACCTT GTCCCCTCCC CTAACTGCAG TATCCAACCA GCACGCAGAC
    ATGATACCGG GTGCTGCTGT AGCAGCTATC GGATCACTTC CTGTGACATC ACTTCCTCCT
    TTGCCCATTG TGGGTGGAGT TCCTGTAGGC ACTGCAACCC TGAGCTCACC CCTAACACCC
    CTAATCAGTT CTGTCAGTAC AATGGGGGGG ACGGTTCCCC CATCTGTCAC ATCTCCCCCA
    GTGCCACAGA TAGCGGGTAG CGTTGCCGGG AGCCTGCCTC CTGCATCAGC CTCCGCCCCT
    CTTATTCATG GCAGTGTTGC CCCATCTCAG GTACCGCTTT GCAGCAGCAG TTCTGTTCCC
    AGCTTTGCAG AGAGCGTGGC AGTCAGCCCC CTGCTGGATA GTGGCCAAAA GGCAGCAGAA
    GTGGGCACGG TAATGAGCAC GACTGGCTTG TCTCTGCCCA TCTCTGCACC TCTGACATAT
    GCTGGGGTGG TTGCAGCATC AGGCATCACA AGTCAACCTG CCGTGCATCT CCCCCAGGGG
    GTGGCCACAA CACCAGTTGT ATCCCAGGCA GTAGGGGGAG CTGTGGGCAC ACCCCTCTCA
    TCTCTTCCTC CCATCCCTGG AGGCATGTCT GTTAGCCAAC CAGGATCAAA TATACCCCCT
    GTGCAACACA CGTTGGCCCA TGGCCAACCC CAGCCTACCC CTCTCCCCAA CCAGCCTCAT
    ACGCACCTTG TAGAGGGTGA CACCGATAAC CTGCCCAAAC CTGGCATAGA CGATATAAAA
    ACCCTGGAGG AGAAGCTGAG GTCGCTGTTC AGTGAGCATG GCAGTGCCGG GGCCCCTCAT
    GGACTCACCC CATCAGAAAC TCAATTGGGT TTGGATGGGG CCCCCCTGCC AGGCCCTTCC
    ACAGGTAGCA CCGCTCAGAC CAAGCCTGCA GCATCCTCTT CTCCACCAAC CAGCTTACCT
    TTAGGGCCAC CTGGAGTGAC GACAATGACT CAGATCATGA CACCTGGACA AGCTGCTACA
    CCTGTCACCT ACGTATCTGC AGCTGGAAGC TCTATAGCCA CGGCCAAAGC TGCCACCCCA
    CCCTCAAAGC CGCCTCTCAG TCGGGTACCG GTGACCACGG CCGGGGCAGA GACCCCAGCA
    GGCAGTCTCC CTGGTGAACA CCTGCCTCCT CCTTCTCTCC CAGGACCTTC ACTAACTCAG
    TCCCAGCAGC CTCTAGAAGA CCTGGATTTC CAGCTGAGAA GGACGTTGAG CCCTGACACT
    GTCCCTGTGC CTTCTGCACT AATATTTCCT GCACACAGCG GAGCTTCTAC CGGTGTTGCA
    GCACCCGTCG GCTCAGCATT GCAACCCTCC GCTTCAGAAT TGCCAAAAGC CAGCGCTGGA
    GAGGCAGAGG TGGGCGCGAG TGTCCAGAGG ATGGGCAGGT TTCAGGTCTC TTTGGCAGCA
    GAGAATGTGC TCAAGCAGAG CGAGGAGACC AAACTGTCCA TCTCAGACAC TTCCTCCACC
    ACTTCTACAT CCTCCTCTTC CTCTGCGTCA TGCAGCAGCC CAGAGAGCAC CCTGACTAAG
    CCCCAGGCCA AAACCGCTGC TCCCTTAGAC ATAGTGGATG GTGTTGGTCC CCACTTGGTG
    CATCTACAGA GCCTATCTCC CAAGCAGTTG GTGAAAGTGG GGCGCTTCCA GGTTACCACT
    GCCACCACGG ACAAAGTGGG GCGCTTTTCT GTGTCCCGGA CACAAGATGA TATATCTGCT
    GGGGTGCTGG AGACAGACTC CCAGAGGTCC TCCTCCCCAC AGACTGAGAA ACATCTGAAT
    GGACCCTACT TACAGCAAGA CTCCTCTGCG GCTCTCGGCT ATCCGGCCAG GGAGACGCAG
    GAAGACAGCC TAGGGAGCCC CGTTCTGCAC CACGCTTTGG GGTCCAAGCT TAGCCTGCAG
    AGCCTTAGCA ACTCGTTCAA CTCTTCCTAC ATGAGCAGCG ATAATGATTC TGACATAGAA
    GATGAAGATC TGAAGAAGGA GCTGCGAAGG CTACGAGACA AGCATCTCAA GGAGATTCAG
    GAACTGCAGA GTCGACAGAA GCAGGAGATC GAGCTGTTGT ACACGGCTTT GGGAAAAGTG
    CCCCCTGCTG TCATTATCCC TCCGGCAGCG CCCCTTTCTG GCCGGCGCAG GAGGCATACC
    AAGGGGAAGG GCAGCAAGTC CAGCCGGGGC AGCTCCCACA ACAGCCGCAG CCCTCATTTG
    TCAGGCGACC TGTCCACGCA GAGCGCTCCG TCGGCATTAC CCCCCCAGCC GGGCCTTCTG
    CTCCCCCCAA GCAGTGCGCC CGAACATGCT CATCTTCTCA AGCCCTCCCC TTCCAGCGAC
    ATTCTCTGCT CAGCCTTCAC TAGCGACGCT GCCCTCTCTG TGCCCAGCCT GTCCACCCAG
    GGCCAAGGGA CCAGCAGCAC TAACACAGTC GCACCATCAA CTGCCCCGGC CCCCCAGCCA
    AACGTGCCCC CCGTGTCATC CAGCCGCAAG GGAACCTTCA CGGACGATCT ACACAAACTG
    GTGGACAACT GGGCCCGAGA CGCCATGAAC CTGACTGGAA AAAAGGCTTG TAAGGCCTCT
    GGGCAACCCA GCTATGAGGG ACCCGGGATG GCCCGTAAGT TCTCTGCTCC CGGACAGCTG
    TGTGTCCCCA TGAACCCATC CCTGGGGGGC TCCGTCCCTC TCCCAGCAGC TTCGGCGACC
    TCCCTGGCTC ACTACCCCAA AGGAATGTGC CCCCCGCAAC AATACGGAAT CCCCGGGGCG
    CTGTTCCAAG CTCCATGGAG TGGCACCGGC GTGCCCCCCT CCTCTCAGCC CCTAAGCCAG
    TTCCCCCCGG CGGTGACATC TTCCTTACAG AACTTCGGTA TTAGCAGCTT GCAGAAATCT
    ATCAGCAACC CCCAAGGATC CAACCTGCGG ACTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754492.1
    CDS326..8284
    Translation

    Target ORF information:

    RefSeq Version XM_031898632.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898632.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCATCGTCC ATGGTCCATT CAGCACTCCC AGACTCTCCG
    CTTTTTTTCC CAACCATTCC CAACCAGCGC CCCATGTCTT TCTCTCCGCC GCCTACTGGC
    TTGTCCCCAA AGGTGCCTGC AACCCAGCGA CGCAAGAGCA CCTCTTTCTT GGAGTCCCAA
    GGCCGCCAGG GGCTATCTCA GATTAGGACA CTCGGGCCAG GTGAGGAGAC CCAGTGCTGG
    AGGGCAGACT ACATGGCAGC TCTAGGTAAA GCATCTGTCC GATCCGAGGA TGCTCACAAG
    CATCAAAACT ATGAGGGATT GGCGTTCCAT GACGCAAGAC ATGTTCCGAC AGTTCCAAAT
    GAAACTCTCT TAAAGCCTGG GGAAGCATTG TATCTAGCCA GTCTGCCCTC ACAGGAGAGC
    TACAGCAGCA TGGCTGCCCC TGAGCATATG GAGGGCCACA AGCTGACCCA GCTTGGCTCG
    TTTTACGATT ATCACACTGG GCAAACCTAC ATTGCGCGGC CCCTGAAGAG CCTAAGACTG
    GAATCTGGGT TAAACCAGCC GTCGGCTTTG GGTAGCATCT CCAACCCAAT CTCCTCCGAC
    CCACACGCCA TGTTTCATCA GATGTTTGTG CCCCAGTCTG CTCCTCCAGT GCTGGCTCAA
    AGCAGCGAAG GCCACCCAGG GTGTGTGTTT GAGTTCCACG TGCACCCATC TGGGTCTGTT
    CCCGGCGAAG CAACCGTTTA CTTGCCACAC CGAGTTTACC GTCCTCGACG GGGCTCTGTA
    GACTTTAACC CAGAAGAACA GCCGCCGGGT TCTACGCTGC AGAGCCGCCT CCAAACTGTG
    ACTGAAGAGC AGTGCCACCA CAATGGCCCA GAACCACTCC CACCCTTTAT AGGATTCATC
    ACATGCAACC GTATGGAGAG CAGCCCCGAC TATTCCAGTG ATTCTTCCCA AAGGAATTCA
    TCCGATCCCG ATTATCTCTC TCCTCCTCTG GCCTTCAGCT CCAATATCTC TATGGCTTCC
    AAGCAGAAAA CGTTGCAGGA TCCACAGATT TTCTTTTGTG TCCCACAGTC CGGAACGACG
    GCTTATGCTG GACCATCAGC TTTGTACAGC CAGCAGCCAC AAATATCATC TGGACAACCA
    CCCACCACAT CCATGGGAAT CAGTGCTCAG CCCGGTCCCC ACACCCAGGG CATGGTGCAT
    CATCAGCCTT CTCTGCATCC AGCCTCGCAG TACCAAGTGC AGCAGCCAAT GCCTACTGTG
    GGTTCCAGTA TTGCGGCCCC ATCACAGCCG GTGATTACCC AAACGCCAGC CCCTCAGCCG
    GGCACCCAGC TCCCTGCCTC CCAGCCTTTG CCTGCTGTTC AAGGAGAGCC TCAGATTCCT
    GCTCAGACAG CTCCTGCTCC CCAGCCCGTT CCTACAGTGC CGGCCTCTCT GCCACCTCAT
    TTTCTTCCCT TGGCACAATC TTTGGTTGCA GTCCCTCTCA TTCCTCCATT CGCTGCCCCA
    CAGTTGCCTT TAACGGTACC ACCCCCGGTC ATACAGACAG GGTTCCCTTC CCTACCCATG
    ACTGTAACCC CAAGTATTGG CCAAACTGTA CCAGGAATAC CCCCATCCTT AGGAGCGCCA
    GTCCTGCCCC ATGGGCAGGC AGTGTTAGTT CCCACTCACA TCACTAATGT TGCTCTCCAG
    CCTTTGGCAC CAATCCCCAC ACCTCCTCTG TGCCCTGTAA CTCAGCCCAT TGGATTACCG
    GCCACCCTGG GAAAAGCTGC TGAGCCCTTG CTTCCTCCGG GAGATACTTC TTACCAGGTT
    TTCCAGGCTA CTCACCCTCT ATTCCCTGGA GATGTAAGCT TTGTTCCTCC CGTTGCCATC
    CCCTCAGCAA TGGTATCTCC CCCTATCCGT GGTGAAAGTT TAGCCCAACC TGGCTTCCAT
    ACTCAGCCTT ATCCTGAAAA CAGCAATTTG TATTCCCATG GAGGGCCCAC TCCCCAGCCC
    GCTGTTCTCA CTACCCAGCC GGTGCCTGCA GGAACCCAGA CGCAACCTGC CGTCGAGGGT
    TCACAGAGTA GCTCCCTGGC AATTTCCCAG GATACCCAGT CATCTCCTCA AGCAACACAA
    ACTGCCTCCT CCATGGACAG CGCTCATTCA GACGTGGCAT CAGGACTGAG TGACGGAAAC
    GAGGGCAGCA CAGGTGGGAG ACACGAAGGC CGAGCCACTA AACGACACTA CCGAAAATCT
    GTGAGAAGCC GCTCCAGGCA CGAGAAAACT GCACGCCCAA AGCTTCGAAT CCTTAATGTT
    TCCAACAAAG GGGATCGTGT TGTAGAGTGT CAGCTGGAGA CTCACAACCG CAAGATGGTC
    ACCTTCAAAT TCGACCTGGA TGGAGACAAT CCAGAGGAGA TTGCTACTAT AATGGTACAG
    AATGTGTTTA TTTTGGCTAC GGAGAGAGAA TCCTTTGTAG AGCAGGTAAA AGACATAATT
    GAGAAGGCCG ATGAAATGTT GAGCGAAGAT GTGAGCGTGG AGCCGGAAGG AGAAACGAGG
    GCAGAAAGTT TGCAAATCAA GGATGATGGG GATTACCCAG AATCTCAGAA GATGAATTCT
    GAATTTCGAA TGCCAGATCC TGTATATTCC CTACCACAGA CAGCAGGGCT TATTCCCAGC
    GTTGTCACAC AAGTCGTTCA TTCTGCCGGA AGACGATTTA TTGTCAGTCC GGTGCCTGAA
    AGCCGTCTAA AAGAACAGTC ATTCTTCAGT ACTTCATTGC AGGACCCTCA GCTACCAGTC
    TCTGTCGCGC ATGGTCCAGG GATGAATTTG TCTCATTCTG CCTCTTCTGT GAGCCTGCAG
    CAAGCCTTTG CAGAACTACG CCAGTCACAG ATGAGCGAGG GTCCTAGCAC AGCTCCTCCC
    ATATTTAACC AAACTGGACC CCCCTTTCCA CCATCCATGA CTAGTCTGGT GGCAACTCTG
    CCTTCTAATG CAATGCCTAT CCTCCAGGCA GCCCCAGTGA TCCCCAGTAG CCTGCCATCT
    TCTGTGCCTC AGCCCCTGGT GGAGGTGGTT GTACCTCAGG CTGCTGGGAT GGCTCCAACT
    AGCAATGTTC CAAGCACCTT GTCCCCTCCC CTAACTGCAG TATCCAACCA GCACGCAGAC
    ATGATACCGG GTGCTGCTGT AGCAGCTATC GGATCACTTC CTGTGACATC ACTTCCTCCT
    TTGCCCATTG TGGGTGGAGT TCCTGTAGGC ACTGCAACCC TGAGCTCACC CCTAACACCC
    CTAATCAGTT CTGTCAGTAC AATGGGGGGG ACGGTTCCCC CATCTGTCAC ATCTCCCCCA
    GTGCCACAGA TAGCGGGTAG CGTTGCCGGG AGCCTGCCTC CTGCATCAGC CTCCGCCCCT
    CTTATTCATG GCAGTGTTGC CCCATCTCAG GTACCGCTTT GCAGCAGCAG TTCTGTTCCC
    AGCTTTGCAG AGAGCGTGGC AGTCAGCCCC CTGCTGGATA GTGGCCAAAA GGCAGCAGAA
    GTGGGCACGG TAATGAGCAC GACTGGCTTG TCTCTGCCCA TCTCTGCACC TCTGACATAT
    GCTGGGGTGG TTGCAGCATC AGGCATCACA AGTCAACCTG CCGTGCATCT CCCCCAGGGG
    GTGGCCACAA CACCAGTTGT ATCCCAGGCA GTAGGGGGAG CTGTGGGCAC ACCCCTCTCA
    TCTCTTCCTC CCATCCCTGG AGGCATGTCT GTTAGCCAAC CAGGATCAAA TATACCCCCT
    GTGCAACACA CGTTGGCCCA TGGCCAACCC CAGCCTACCC CTCTCCCCAA CCAGCCTCAT
    ACGCACCTTG TAGAGGGTGA CACCGATAAC CTGCCCAAAC CTGGCATAGA CGATATAAAA
    ACCCTGGAGG AGAAGCTGAG GTCGCTGTTC AGTGAGCATG GCAGTGCCGG GGCCCCTCAT
    GGACTCACCC CATCAGAAAC TCAATTGGGT TTGGATGGGG CCCCCCTGCC AGGCCCTTCC
    ACAGGTAGCA CCGCTCAGAC CAAGCCTGCA GCATCCTCTT CTCCACCAAC CAGCTTACCT
    TTAGGGCCAC CTGGAGTGAC GACAATGACT CAGATCATGA CACCTGGACA AGCTGCTACA
    CCTGTCACCT ACGTATCTGC AGCTGGAAGC TCTATAGCCA CGGCCAAAGC TGCCACCCCA
    CCCTCAAAGC CGCCTCTCAG TCGGGTACCG GTGACCACGG CCGGGGCAGA GACCCCAGCA
    GGCAGTCTCC CTGGTGAACA CCTGCCTCCT CCTTCTCTCC CAGGACCTTC ACTAACTCAG
    TCCCAGCAGC CTCTAGAAGA CCTGGATTTC CAGCTGAGAA GGACGTTGAG CCCTGACACT
    GTCCCTGTGC CTTCTGCACT AATATTTCCT GCACACAGCG GAGCTTCTAC CGGTGTTGCA
    GCACCCGTCG GCTCAGCATT GCAACCCTCC GCTTCAGAAT TGCCAAAAGC CAGCGCTGGA
    GAGGCAGAGG TGGGCGCGAG TGTCCAGAGG ATGGGCAGGT TTCAGGTCTC TTTGGCAGCA
    GAGAATGTGC TCAAGCAGAG CGAGGAGACC AAACTGTCCA TCTCAGACAC TTCCTCCACC
    ACTTCTACAT CCTCCTCTTC CTCTGCGTCA TGCAGCAGCC CAGAGAGCAC CCTGACTAAG
    CCCCAGGCCA AAACCGCTGC TCCCTTAGAC ATAGTGGATG GTGTTGGTCC CCACTTGGTG
    CATCTACAGA GCCTATCTCC CAAGCAGTTG GTGAAAGTGG GGCGCTTCCA GGTTACCACT
    GCCACCACGG ACAAAGTGGG GCGCTTTTCT GTGTCCCGGA CACAAGATGA TATATCTGCT
    GGGGTGCTGG AGACAGACTC CCAGAGGTCC TCCTCCCCAC AGACTGAGAA ACATCTGAAT
    GGACCCTACT TACAGCAAGA CTCCTCTGCG GCTCTCGGCT ATCCGGCCAG GGAGACGCAG
    GAAGACAGCC TAGGGAGCCC CGTTCTGCAC CACGCTTTGG GGTCCAAGCT TAGCCTGCAG
    AGCCTTAGCA ACTCGTTCAA CTCTTCCTAC ATGAGCAGCG ATAATGATTC TGACATAGAA
    GATGAAGATC TGAAGAAGGA GCTGCGAAGG CTACGAGACA AGCATCTCAA GGAGATTCAG
    GAACTGCAGA GTCGACAGAA GCAGGAGATC GAGCTGTTGT ACACGGCTTT GGGAAAAGTG
    CCCCCTGCTG TCATTATCCC TCCGGCAGCG CCCCTTTCTG GCCGGCGCAG GAGGCATACC
    AAGGGGAAGG GCAGCAAGTC CAGCCGGGGC AGCTCCCACA ACAGCCGCAG CCCTCATTTG
    TCAGGCGACC TGTCCACGCA GAGCGCTCCG TCGGCATTAC CCCCCCAGCC GGGCCTTCTG
    CTCCCCCCAA GCAGTGCGCC CGAACATGCT CATCTTCTCA AGCCCTCCCC TTCCAGCGAC
    ATTCTCTGCT CAGCCTTCAC TAGCGACGCT GCCCTCTCTG TGCCCAGCCT GTCCACCCAG
    GGCCAAGGGA CCAGCAGCAC TAACACAGTC GCACCATCAA CTGCCCCGGC CCCCCAGCCA
    AACGTGCCCC CCGTGTCATC CAGCCGCAAG GGAACCTTCA CGGACGATCT ACACAAACTG
    GTGGACAACT GGGCCCGAGA CGCCATGAAC CTGACTGGAA AAAAGGCTTG TAAGGCCTCT
    GGGCAACCCA GCTATGAGGG ACCCGGGATG GCCCGTAAGT TCTCTGCTCC CGGACAGCTG
    TGTGTCCCCA TGAACCCATC CCTGGGGGGC TCCGTCCCTC TCCCAGCAGC TTCGGCGACC
    TCCCTGGCTC ACTACCCCAA AGGAATGTGC CCCCCGCAAC AATACGGAAT CCCCGGGGCG
    CTGTTCCAAG CTCCATGGAG TGGCACCGGC GTGCCCCCCT CCTCTCAGCC CCTAAGCCAG
    TTCCCCCCGG CGGTGACATC TTCCTTACAG AACTTCGGTA TTAGCAGCTT GCAGAAATCT
    ATCAGCAACC CCCAAGGATC CAACCTGCGG ACTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa23040
    Clone ID Related Accession (Same CDS sequence) NM_001097234.1 , XM_031898630.1
    Accession Version NM_001097234.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6309bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-27
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC128628.1. ##Evidence-Data-START## Transcript exon combination :: BC128628.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1696413, SAMEA1696415 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCACAAATA TCATCTGGAC AACCACCCAC CACATCCATG
    GGAATCAGTG CTCAGCCCGG TCCCCACACC CAGGGCATGG TGCATCATCA GCCTTCTCTG
    CATCCAGCCT CGCAGTACCA AGTGCAGCAG CCAATGCCTA CTGTGGGTTC CAGTATTGCG
    GCCCCATCAC AGCCGGTGAT TACCCAAACG CCAGCCCCTC AGCCGGGCAC CCAGGTTTTC
    CAGGCTACTC ACCCTCTATT CCCTGGAGAT GTAAGCTTTG TTCCTCCCGT TGCCATCCCC
    TCAGCAATGG TATCTCCCCC TATCCGTGGT GAAAGTTTAG CCCAACCTGG CTTCCATACT
    CAGCCTTATC CTGAAAACAG CAATTTGTAT TCCCATGGAG GGCCCACTCC CCAGCCCGCT
    GTTCTCACTA CCCAGCCGGT GCCTGCAGGA ACCCAGACGC AACCTGCCGT CGAGGGTTCA
    CAGAGTAGCT CCCTGGCAAT TTCCCAGGAT ACCCAGTCAT CTCCTCAAGC AACACAAACT
    GCCTCCTCCA TGGACAGCGC TCATTCAGAC GTGGCATCAG GACTGAGTGA CGGAAACGAG
    GGCAGCACAG GTGGGAGACA CGAAGGCCGA GCCACTAAAC GACACTACCG AAAATCTGTG
    AGAAGCCGCT CCAGGCACGA GAAAACTGCA CGCCCAAAGC TTCGAATCCT TAATGTTTCC
    AACAAAGGGG ATCGTGTTGT AGAGTGTCAG CTGGAGACTC ACAACCGCAA GATGGTCACC
    TTCAAATTCG ACCTGGATGG AGACAATCCA GAGGAGATTG CTACTATAAT GGTACAGAAT
    GTGTTTATTT TGGCTACGGA GAGAGAATCC TTTGTAGAGC AGGTAAAAGA CATAATTGAG
    AAGGCCGATG AAATGTTGAG CGAAGATGTG AGCGTGGAGC CGGAAGGAGA AACGAGGGCA
    GAAAGTTTGC AAATCAAGGA TGATGGGGAT TACCCAGAAT CTCAGAAGAT GAATTCTGAA
    TTTCGAATGC CAGATCCTGT ATATTCCCTA CCACAGACAG CAGGGCTTAT TCCCAGCGTT
    GTCACACAAG TCGTTCATTC TGCCGGAAGA CGATTTATTG TCAGTCCGGT GCCTGAAAGC
    CGTCTAAAAG AACAGTCATT CTTCAGTACT TCATTGCAGG ACCCTCAGCT ACCAGTCTCT
    GTCGCGCATG GTCCAGGGAT GAATTTGTCT CATTCTGCCT CTTCTGTGAG CCTGCAGCAA
    GCCTTTGCAG AACTACGCCA GTCACAGATG AGCGAGGGTC CTAGCACAGC TCCTCCCATA
    TTTAACCAAA CTGGACCCCC CTTTCCACCA TCCATGACTA GTCTGGTGGC AACTCTGCCT
    TCTAATGCAA TGCCTATCCT CCAGGCAGCC CCAGTGATCC CCAGTAGCCT GCCATCTTCT
    GTGCCTCAGC CCCTGGTGGA GGTGGTTGTA CCTCAGGCTG CTGGGATGGC TCCAACTAGC
    AATGTTCCAA GCACCTTGTC CCCTCCCCTA ACTGCAGTAT CCAACCAGCA CGCAGACATG
    ATACCGGGTG CTGCTGTAGC AGCTATCGGA TCACTTCCTG TGACATCACT TCCTCCTTTG
    CCCATTGTGG GTGGAGTTCC TGTAGGCACT GCAACCCTGA GCTCACCCCT AACACCCCTA
    ATCAGTTCTG TCAGTACAAT GGGGGGGACG GTTCCCCCAT CTGTCACATC TCCCCCAGTG
    CCACAGATAG CGGGTAGCGT TGCCGGGAGC CTGCCTCCTG CATCAGCCTC CGCCCCTCTT
    ATTCATGGCA GTGTTGCCCC ATCTCAGGTA CCGCTTTGCA GCAGCAGTTC TGTTCCCAGC
    TTTGCAGAGA GCGTGGCAGT CAGCCCCCTG CTGGATAGTG GCCAAAAGGC AGCAGAAGTG
    GGCACGGTAA TGAGCACGAC TGGCTTGTCT CTGCCCATCT CTGCACCTCT GACATATGCT
    GGGGTGGTTG CAGCATCAGG CATCACAAGT CAACCTGCCG TGCATCTCCC CCAGGGGGTG
    GCCACAACAC CAGTTGTATC CCAGGCAGTA GGGGGAGCTG TGGGCACACC CCTCTCATCT
    CTTCCTCCCA TCCCTGGAGG CATGTCTGTT AGCCAACCAG GATCAAATAT ACCCCCTGTG
    CAACACACGT TGGCCCATGG CCAACCCCAG CCTACCCCTC TCCCCAACCA GCCTCATACG
    CACCTTGTAG AGGGTGACAC CGATAACCTG CCCAAACCTG GCATAGACGA TATAAAAACC
    CTGGAGGAGA AGCTGAGGTC GCTGTTCAGT GAGCATGGCA GTGCCGGGGC CCCTCATGGA
    CTCACCCCAT CAGAAACTCA ATTGGGTTTG GATGGGGCCC CCCTGCCAGG CCCTTCCACA
    GGTAGCACCG CTCAGACCAA GCCTGCAGCA TCCTCTTCTC CACCAACCAG CTTACCTTTA
    GGGCCACCTG GAGTGACGAC AATGACTCAG ATCATGACAC CTGGACAAGC TGCTACACCT
    GTCACCTACG TATCTGCAGC TGGAAGCTCT ATAGCCACGG CCAAAGCTGC CACCCCACCC
    TCAAAGCCGC CTCTCAGTCG GGTACCGGTG ACCACGGCCG GGGCAGAGAC CCCAGCAGGC
    AGTCTCCCTG GTGAACACCT GCCTCCTCCT TCTCTCCCAG GACCTTCACT AACTCAGCCT
    GCACACAGCG GAGCTTCTAC CGGTGTTGCA GCACCCGTCG GCTCAGCATT GCAACCCTCC
    GCTTCAGAAT TGCCAAAAGC CAGCGCTGGA GAGGCAGAGG TGGGCGCGAG TGTCCAGAGG
    ATGGGCAGGT TTCAGGTCTC TTTGGCAGCA GAGAATGTGC TCAAGCAGAG CGAGGAGACC
    AAACTGTCCA TCTCAGACAC TTCCTCCACC ACTTCTACAT CCTCCTCTTC CTCTGCGTCA
    TGCAGCAGCC CAGAGAGCAC CCTGACTAAG CCCCAGGCCA AAACCGCTGC TCCCTTAGAC
    ATAGTGGATG GTGTTGGTCC CCACTTGGTG CATCTACAGA GCCTATCTCC CAAGCAGTTG
    GTGAAAGTGG GGCGCTTCCA GGTTACCACT GCCACCACGG ACAAAGTGGG GCGCTTTTCT
    GTGTCCCGGA CACAAGATGA TATATCTGCT GGGGTGCTGG AGACAGACTC CCAGAGGTCC
    TCCTCCCCAC AGACTGAGAA ACATCTGAAT GGACCCTACT TACAGCAAGA CTCCTCTGCG
    GCTCTCGGCT ATCCGGCCAG GGAGACGCAG GAAGACAGCC TAGGGAGCCC CGTTCTGCAC
    CACGCTTTGG GGTCCAAGCT TAGCCTGCAG AGCCTTAGCA ACTCGTTCAA CTCTTCCTAC
    ATGAGCAGCG ATAATGATTC TGACATAGAA GATGAAGATC TGAAGAAGGA GCTGCGAAGG
    CTACGAGACA AGCATCTCAA GGAGATTCAG GAACTGCAGA GTCGACAGAA GCAGGAGATC
    GAGCTGTTGT ACACGGCTTT GGGAAAAGTG CCCCCTGCTG TCATTATCCC TCCGGCAGCG
    CCCCTTTCTG GCCGGCGCAG GAGGCATACC AAGGGGAAGG GCAGCAAGTC CAGCCGGGGC
    AGCTCCCACA ACAGCCGCAG CCCTCATTTG TCAGGCGACC TGTCCACGCA GAGCGCTCCG
    TCGGCATTAC CCCCCCAGCC GGGCCTTCTG CTCCCCCCAA GCAGTGCGCC CGAACATGCT
    CATCTTCTCA AGCCCTCCCC TTCCAGCGAC ATTCTCTGCT CAGCCTTCAC TAGCGACGCT
    GCCCTCTCTG TGCCCAGCCT GTCCACCCAG GGCCAAGGGA CCAGCAGCAC TAACACAGTC
    GCACCATCAA CTGCCCCGGC CCCCCAGCCA AACGTGCCCC CCGTGTCATC CAGCCGCAAG
    GGAACCTTCA CGGACGATCT ACACAAACTG GTGGACAACT GGGCCCGAGA CGCCATGAAC
    CTGACTGGAA AAAAGGCTTG TAAGGCCTCT GGGCAACCCA GCTATGAGGG ACCCGGGATG
    GCCCGTAAGT TCTCTGCTCC CGGACAGCTG TGTGTCCCCA TGAACCCATC CCTGGGGGGC
    TCCGTCCCTC TCCCAGCAGC TTCGGCGACC TCCCTGGCTC ACTACCCCAA AGGAATGTGC
    CCCCCGCAAC AATACGGAAT CCCCGGGGCG CTGTTCCAAG CTCCATGGAG TGGCACCGGC
    GTGCCCCCCT CCTCTCAGCC CCTAAGCCAG TTCCCCCCGG CGGTGACATC TTCCTTACAG
    AACTTCGGTA TTAGCAGCTT GCAGAAATCT ATCAGCAACC CCCAAGGATC CAACCTGCGG
    ACTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001090703.1
    CDS319..6627
    Translation

    Target ORF information:

    RefSeq Version NM_001097234.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001097234.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCACAAATA TCATCTGGAC AACCACCCAC CACATCCATG
    GGAATCAGTG CTCAGCCCGG TCCCCACACC CAGGGCATGG TGCATCATCA GCCTTCTCTG
    CATCCAGCCT CGCAGTACCA AGTGCAGCAG CCAATGCCTA CTGTGGGTTC CAGTATTGCG
    GCCCCATCAC AGCCGGTGAT TACCCAAACG CCAGCCCCTC AGCCGGGCAC CCAGGTTTTC
    CAGGCTACTC ACCCTCTATT CCCTGGAGAT GTAAGCTTTG TTCCTCCCGT TGCCATCCCC
    TCAGCAATGG TATCTCCCCC TATCCGTGGT GAAAGTTTAG CCCAACCTGG CTTCCATACT
    CAGCCTTATC CTGAAAACAG CAATTTGTAT TCCCATGGAG GGCCCACTCC CCAGCCCGCT
    GTTCTCACTA CCCAGCCGGT GCCTGCAGGA ACCCAGACGC AACCTGCCGT CGAGGGTTCA
    CAGAGTAGCT CCCTGGCAAT TTCCCAGGAT ACCCAGTCAT CTCCTCAAGC AACACAAACT
    GCCTCCTCCA TGGACAGCGC TCATTCAGAC GTGGCATCAG GACTGAGTGA CGGAAACGAG
    GGCAGCACAG GTGGGAGACA CGAAGGCCGA GCCACTAAAC GACACTACCG AAAATCTGTG
    AGAAGCCGCT CCAGGCACGA GAAAACTGCA CGCCCAAAGC TTCGAATCCT TAATGTTTCC
    AACAAAGGGG ATCGTGTTGT AGAGTGTCAG CTGGAGACTC ACAACCGCAA GATGGTCACC
    TTCAAATTCG ACCTGGATGG AGACAATCCA GAGGAGATTG CTACTATAAT GGTACAGAAT
    GTGTTTATTT TGGCTACGGA GAGAGAATCC TTTGTAGAGC AGGTAAAAGA CATAATTGAG
    AAGGCCGATG AAATGTTGAG CGAAGATGTG AGCGTGGAGC CGGAAGGAGA AACGAGGGCA
    GAAAGTTTGC AAATCAAGGA TGATGGGGAT TACCCAGAAT CTCAGAAGAT GAATTCTGAA
    TTTCGAATGC CAGATCCTGT ATATTCCCTA CCACAGACAG CAGGGCTTAT TCCCAGCGTT
    GTCACACAAG TCGTTCATTC TGCCGGAAGA CGATTTATTG TCAGTCCGGT GCCTGAAAGC
    CGTCTAAAAG AACAGTCATT CTTCAGTACT TCATTGCAGG ACCCTCAGCT ACCAGTCTCT
    GTCGCGCATG GTCCAGGGAT GAATTTGTCT CATTCTGCCT CTTCTGTGAG CCTGCAGCAA
    GCCTTTGCAG AACTACGCCA GTCACAGATG AGCGAGGGTC CTAGCACAGC TCCTCCCATA
    TTTAACCAAA CTGGACCCCC CTTTCCACCA TCCATGACTA GTCTGGTGGC AACTCTGCCT
    TCTAATGCAA TGCCTATCCT CCAGGCAGCC CCAGTGATCC CCAGTAGCCT GCCATCTTCT
    GTGCCTCAGC CCCTGGTGGA GGTGGTTGTA CCTCAGGCTG CTGGGATGGC TCCAACTAGC
    AATGTTCCAA GCACCTTGTC CCCTCCCCTA ACTGCAGTAT CCAACCAGCA CGCAGACATG
    ATACCGGGTG CTGCTGTAGC AGCTATCGGA TCACTTCCTG TGACATCACT TCCTCCTTTG
    CCCATTGTGG GTGGAGTTCC TGTAGGCACT GCAACCCTGA GCTCACCCCT AACACCCCTA
    ATCAGTTCTG TCAGTACAAT GGGGGGGACG GTTCCCCCAT CTGTCACATC TCCCCCAGTG
    CCACAGATAG CGGGTAGCGT TGCCGGGAGC CTGCCTCCTG CATCAGCCTC CGCCCCTCTT
    ATTCATGGCA GTGTTGCCCC ATCTCAGGTA CCGCTTTGCA GCAGCAGTTC TGTTCCCAGC
    TTTGCAGAGA GCGTGGCAGT CAGCCCCCTG CTGGATAGTG GCCAAAAGGC AGCAGAAGTG
    GGCACGGTAA TGAGCACGAC TGGCTTGTCT CTGCCCATCT CTGCACCTCT GACATATGCT
    GGGGTGGTTG CAGCATCAGG CATCACAAGT CAACCTGCCG TGCATCTCCC CCAGGGGGTG
    GCCACAACAC CAGTTGTATC CCAGGCAGTA GGGGGAGCTG TGGGCACACC CCTCTCATCT
    CTTCCTCCCA TCCCTGGAGG CATGTCTGTT AGCCAACCAG GATCAAATAT ACCCCCTGTG
    CAACACACGT TGGCCCATGG CCAACCCCAG CCTACCCCTC TCCCCAACCA GCCTCATACG
    CACCTTGTAG AGGGTGACAC CGATAACCTG CCCAAACCTG GCATAGACGA TATAAAAACC
    CTGGAGGAGA AGCTGAGGTC GCTGTTCAGT GAGCATGGCA GTGCCGGGGC CCCTCATGGA
    CTCACCCCAT CAGAAACTCA ATTGGGTTTG GATGGGGCCC CCCTGCCAGG CCCTTCCACA
    GGTAGCACCG CTCAGACCAA GCCTGCAGCA TCCTCTTCTC CACCAACCAG CTTACCTTTA
    GGGCCACCTG GAGTGACGAC AATGACTCAG ATCATGACAC CTGGACAAGC TGCTACACCT
    GTCACCTACG TATCTGCAGC TGGAAGCTCT ATAGCCACGG CCAAAGCTGC CACCCCACCC
    TCAAAGCCGC CTCTCAGTCG GGTACCGGTG ACCACGGCCG GGGCAGAGAC CCCAGCAGGC
    AGTCTCCCTG GTGAACACCT GCCTCCTCCT TCTCTCCCAG GACCTTCACT AACTCAGCCT
    GCACACAGCG GAGCTTCTAC CGGTGTTGCA GCACCCGTCG GCTCAGCATT GCAACCCTCC
    GCTTCAGAAT TGCCAAAAGC CAGCGCTGGA GAGGCAGAGG TGGGCGCGAG TGTCCAGAGG
    ATGGGCAGGT TTCAGGTCTC TTTGGCAGCA GAGAATGTGC TCAAGCAGAG CGAGGAGACC
    AAACTGTCCA TCTCAGACAC TTCCTCCACC ACTTCTACAT CCTCCTCTTC CTCTGCGTCA
    TGCAGCAGCC CAGAGAGCAC CCTGACTAAG CCCCAGGCCA AAACCGCTGC TCCCTTAGAC
    ATAGTGGATG GTGTTGGTCC CCACTTGGTG CATCTACAGA GCCTATCTCC CAAGCAGTTG
    GTGAAAGTGG GGCGCTTCCA GGTTACCACT GCCACCACGG ACAAAGTGGG GCGCTTTTCT
    GTGTCCCGGA CACAAGATGA TATATCTGCT GGGGTGCTGG AGACAGACTC CCAGAGGTCC
    TCCTCCCCAC AGACTGAGAA ACATCTGAAT GGACCCTACT TACAGCAAGA CTCCTCTGCG
    GCTCTCGGCT ATCCGGCCAG GGAGACGCAG GAAGACAGCC TAGGGAGCCC CGTTCTGCAC
    CACGCTTTGG GGTCCAAGCT TAGCCTGCAG AGCCTTAGCA ACTCGTTCAA CTCTTCCTAC
    ATGAGCAGCG ATAATGATTC TGACATAGAA GATGAAGATC TGAAGAAGGA GCTGCGAAGG
    CTACGAGACA AGCATCTCAA GGAGATTCAG GAACTGCAGA GTCGACAGAA GCAGGAGATC
    GAGCTGTTGT ACACGGCTTT GGGAAAAGTG CCCCCTGCTG TCATTATCCC TCCGGCAGCG
    CCCCTTTCTG GCCGGCGCAG GAGGCATACC AAGGGGAAGG GCAGCAAGTC CAGCCGGGGC
    AGCTCCCACA ACAGCCGCAG CCCTCATTTG TCAGGCGACC TGTCCACGCA GAGCGCTCCG
    TCGGCATTAC CCCCCCAGCC GGGCCTTCTG CTCCCCCCAA GCAGTGCGCC CGAACATGCT
    CATCTTCTCA AGCCCTCCCC TTCCAGCGAC ATTCTCTGCT CAGCCTTCAC TAGCGACGCT
    GCCCTCTCTG TGCCCAGCCT GTCCACCCAG GGCCAAGGGA CCAGCAGCAC TAACACAGTC
    GCACCATCAA CTGCCCCGGC CCCCCAGCCA AACGTGCCCC CCGTGTCATC CAGCCGCAAG
    GGAACCTTCA CGGACGATCT ACACAAACTG GTGGACAACT GGGCCCGAGA CGCCATGAAC
    CTGACTGGAA AAAAGGCTTG TAAGGCCTCT GGGCAACCCA GCTATGAGGG ACCCGGGATG
    GCCCGTAAGT TCTCTGCTCC CGGACAGCTG TGTGTCCCCA TGAACCCATC CCTGGGGGGC
    TCCGTCCCTC TCCCAGCAGC TTCGGCGACC TCCCTGGCTC ACTACCCCAA AGGAATGTGC
    CCCCCGCAAC AATACGGAAT CCCCGGGGCG CTGTTCCAAG CTCCATGGAG TGGCACCGGC
    GTGCCCCCCT CCTCTCAGCC CCTAAGCCAG TTCCCCCCGG CGGTGACATC TTCCTTACAG
    AACTTCGGTA TTAGCAGCTT GCAGAAATCT ATCAGCAACC CCCAAGGATC CAACCTGCGG
    ACTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54257
    Clone ID Related Accession (Same CDS sequence) XM_031898616.1
    Accession Version XM_031898616.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGGTGACC
    ACGGCCGGGG CAGAGACCCC AGCAGGCAGT CTCCCTGGTG AACACCTGCC TCCTCCTTCT
    CTCCCAGGAC CTTCACTAAC TCAGTCCCAG CAGCCTCTAG AAGACCTGGA TTTCCAGCTG
    AGAAGGACGT TGAGCCCTGA CACTGTCCCT GTGCCTTCTG CACTAATATT TCCTGCACAC
    AGCGGAGCTT CTACCGGTGT TGCAGCACCC GTCGGCTCAG CATTGCAACC CTCCGCTTCA
    GAATTGCCAA AAGCCAGCGC TGGAGAGGCA GAGGTGGGCG CGAGTGTCCA GAGGATGGGC
    AGGTTTCAGG TCTCTTTGGC AGCAGAGAAT GTGCTCAAGC AGAGCGAGGA GACCAAACTG
    TCCATCTCAG ACACTTCCTC CACCACTTCT ACATCCTCCT CTTCCTCTGC GTCATGCAGC
    AGCCCAGAGA GCACCCTGAC TAAGCCCCAG GCCAAAACCG CTGCTCCCTT AGACATAGTG
    GATGGTGTTG GTCCCCACTT GGTGCATCTA CAGAGCCTAT CTCCCAAGCA GTTGGTGAAA
    GTGGGGCGCT TCCAGGTTAC CACTGCCACC ACGGACAAAG TGGGGCGCTT TTCTGTGTCC
    CGGACACAAG ATGATATATC TGCTGGGGTG CTGGAGACAG ACTCCCAGAG GTCCTCCTCC
    CCACAGACTG AGAAACATCT GAATGGACCC TACTTACAGC AAGACTCCTC TGCGGCTCTC
    GGCTATCCGG CCAGGGAGAC GCAGGAAGAC AGCCTAGGGA GCCCCGTTCT GCACCACGCT
    TTGGGGTCCA AGCTTAGCCT GCAGAGCCTT AGCAACTCGT TCAACTCTTC CTACATGAGC
    AGCGATAATG ATTCTGACAT AGAAGATGAA GATCTGAAGA AGGAGCTGCG AAGGCTACGA
    GACAAGCATC TCAAGGAGAT TCAGGAACTG CAGAGTCGAC AGAAGCAGGA GATCGAGCTG
    TTGTACACGG CTTTGGGAAA AGTGCCCCCT GCTGTCATTA TCCCTCCGGC AGCGCCCCTT
    TCTGGCCGGC GCAGGAGGCA TACCAAGGGG AAGGGCAGCA AGTCCAGCCG GGGCAGCTCC
    CACAACAGCC GCAGCCCTCA TTTGTCAGGC GACCTGTCCA CGCAGAGCGC TCCGTCGGCA
    TTACCCCCCC AGCCGGGCCT TCTGCTCCCC CCAAGCAGTG CGCCCGAACA TGCTCATCTT
    CTCAAGCCCT CCCCTTCCAG CGACATTCTC TGCTCAGCCT TCACTAGCGA CGCTGCCCTC
    TCTGTGCCCA GCCTGTCCAC CCAGGGCCAA GGCTGGGCCA AGTTTAACTG TGAGTCAGAG
    CGGGTATCCT TCAAAATGGC TGGCAGGAGG ACGCGATTTC TGAGTACGCC CTGCTTGGCT
    CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACT
    AACACAGTCG CACCATCAAC TGCCCCGGCC CCCCAGCCAA ACGTGCCCCC CGTGTCATCC
    AGCCGCAAGG GAACCTTCAC GGACGATCTA CACAAACTGG TGGACAACTG GGCCCGAGAC
    GCCATGAACC TGACTGGAAA AAAGGCTTGT AAGGCCTCTG GGCAACCCAG CTATGAGGGA
    CCCGGGATGG CCCGTAAGTT CTCTGCTCCC GGACAGCTGT GTGTCCCCAT GAACCCATCC
    CTGGGGGGCT CCGTCCCTCT CCCAGCAGCT TCGGCGACCT CCCTGGCTCA CTACCCCAAA
    GGAATGTGCC CCCCGCAACA ATACGGAATC CCCGGGGCGC TGTTCCAAGC TCCATGGAGT
    GGCACCGGCG TGCCCCCCTC CTCTCAGCCC CTAAGCCAGT TCCCCCCGGC GGTGACATCT
    TCCTTACAGA ACTTCGGTAT TAGCAGCTTG CAGAAATCTA TCAGCAACCC CCAAGGATCC
    AACCTGCGGA CTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754476.1
    CDS328..8685
    Translation

    Target ORF information:

    RefSeq Version XM_031898616.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898616.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGGTGACC
    ACGGCCGGGG CAGAGACCCC AGCAGGCAGT CTCCCTGGTG AACACCTGCC TCCTCCTTCT
    CTCCCAGGAC CTTCACTAAC TCAGTCCCAG CAGCCTCTAG AAGACCTGGA TTTCCAGCTG
    AGAAGGACGT TGAGCCCTGA CACTGTCCCT GTGCCTTCTG CACTAATATT TCCTGCACAC
    AGCGGAGCTT CTACCGGTGT TGCAGCACCC GTCGGCTCAG CATTGCAACC CTCCGCTTCA
    GAATTGCCAA AAGCCAGCGC TGGAGAGGCA GAGGTGGGCG CGAGTGTCCA GAGGATGGGC
    AGGTTTCAGG TCTCTTTGGC AGCAGAGAAT GTGCTCAAGC AGAGCGAGGA GACCAAACTG
    TCCATCTCAG ACACTTCCTC CACCACTTCT ACATCCTCCT CTTCCTCTGC GTCATGCAGC
    AGCCCAGAGA GCACCCTGAC TAAGCCCCAG GCCAAAACCG CTGCTCCCTT AGACATAGTG
    GATGGTGTTG GTCCCCACTT GGTGCATCTA CAGAGCCTAT CTCCCAAGCA GTTGGTGAAA
    GTGGGGCGCT TCCAGGTTAC CACTGCCACC ACGGACAAAG TGGGGCGCTT TTCTGTGTCC
    CGGACACAAG ATGATATATC TGCTGGGGTG CTGGAGACAG ACTCCCAGAG GTCCTCCTCC
    CCACAGACTG AGAAACATCT GAATGGACCC TACTTACAGC AAGACTCCTC TGCGGCTCTC
    GGCTATCCGG CCAGGGAGAC GCAGGAAGAC AGCCTAGGGA GCCCCGTTCT GCACCACGCT
    TTGGGGTCCA AGCTTAGCCT GCAGAGCCTT AGCAACTCGT TCAACTCTTC CTACATGAGC
    AGCGATAATG ATTCTGACAT AGAAGATGAA GATCTGAAGA AGGAGCTGCG AAGGCTACGA
    GACAAGCATC TCAAGGAGAT TCAGGAACTG CAGAGTCGAC AGAAGCAGGA GATCGAGCTG
    TTGTACACGG CTTTGGGAAA AGTGCCCCCT GCTGTCATTA TCCCTCCGGC AGCGCCCCTT
    TCTGGCCGGC GCAGGAGGCA TACCAAGGGG AAGGGCAGCA AGTCCAGCCG GGGCAGCTCC
    CACAACAGCC GCAGCCCTCA TTTGTCAGGC GACCTGTCCA CGCAGAGCGC TCCGTCGGCA
    TTACCCCCCC AGCCGGGCCT TCTGCTCCCC CCAAGCAGTG CGCCCGAACA TGCTCATCTT
    CTCAAGCCCT CCCCTTCCAG CGACATTCTC TGCTCAGCCT TCACTAGCGA CGCTGCCCTC
    TCTGTGCCCA GCCTGTCCAC CCAGGGCCAA GGCTGGGCCA AGTTTAACTG TGAGTCAGAG
    CGGGTATCCT TCAAAATGGC TGGCAGGAGG ACGCGATTTC TGAGTACGCC CTGCTTGGCT
    CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACT
    AACACAGTCG CACCATCAAC TGCCCCGGCC CCCCAGCCAA ACGTGCCCCC CGTGTCATCC
    AGCCGCAAGG GAACCTTCAC GGACGATCTA CACAAACTGG TGGACAACTG GGCCCGAGAC
    GCCATGAACC TGACTGGAAA AAAGGCTTGT AAGGCCTCTG GGCAACCCAG CTATGAGGGA
    CCCGGGATGG CCCGTAAGTT CTCTGCTCCC GGACAGCTGT GTGTCCCCAT GAACCCATCC
    CTGGGGGGCT CCGTCCCTCT CCCAGCAGCT TCGGCGACCT CCCTGGCTCA CTACCCCAAA
    GGAATGTGCC CCCCGCAACA ATACGGAATC CCCGGGGCGC TGTTCCAAGC TCCATGGAGT
    GGCACCGGCG TGCCCCCCTC CTCTCAGCCC CTAAGCCAGT TCCCCCCGGC GGTGACATCT
    TCCTTACAGA ACTTCGGTAT TAGCAGCTTG CAGAAATCTA TCAGCAACCC CCAAGGATCC
    AACCTGCGGA CTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54258
    Clone ID Related Accession (Same CDS sequence) XM_031898617.1
    Accession Version XM_031898617.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCTTGTCGA GGTCGTAGCA TGTCAGTATG TGTTCCCAAC
    CTTTCCTCTG CTCCCCCACT CCTTTCCGTT GTGCCCCCCA GTGCTCCAGG CTCCCCTTCC
    CCATTGCAGT CTGCCCCTGC CTCCCCACTC TGGACATCTC CCGAAGATTC CTTTGCTGAA
    AAGCTTTCCA AGGCTCTAGA GAGTGTTCTG CCCATGCACT CCTCCAGCCC TCGCAAGCAC
    CAACGCCGCT GCAGCCTGCC TACCCTCTAT ACCAGCCCTC CATCGTCCAT GGTCCATTCA
    GCACTCCCAG ACTCTCCGCT TTTTTTCCCA ACCATTCCCA ACCAGCGCCC CATGTCTTTC
    TCTCCGCCGC CTACTGGCTT GTCCCCAAAG GTGCCTGCAA CCCAGCGACG CAAGAGCACC
    TCTTTCTTGG AGTCCCAAGG CCGCCAGGGG CTATCTCAGA TTAGGACACT CGGGCCAGGT
    GAGGAGACCC AGTGCTGGAG GGCAGACTAC ATGGCAGCTC TAGGTAAAGC ATCTGTCCGA
    TCCGAGGATG CTCACAAGCA TCAAAACTAT GAGGGATTGG CGTTCCATGA CGCAAGACAT
    GTTCCGACAG TTCCAAATGA AACTCTCTTA AAGCCTGGGG AAGCATTGTA TCTAGCCAGT
    CTGCCCTCAC AGGAGAGCTA CAGCAGCATG GCTGCCCCTG AGCATATGGA GGGCCACAAG
    CTGACCCAGC TTGGCTCGTT TTACGATTAT CACACTGGGC AAACCTACAT TGCGCGGCCC
    CTGAAGAGCC TAAGACTGGA ATCTGGGTTA AACCAGCCGT CGGCTTTGGG TAGCATCTCC
    AACCCAATCT CCTCCGACCC ACACGCCATG TTTCATCAGA TGTTTGTGCC CCAGTCTGCT
    CCTCCAGTGC TGGCTCAAAG CAGCGAAGGC CACCCAGGGT GTGTGTTTGA GTTCCACGTG
    CACCCATCTG GGTCTGTTCC CGGCGAAGCA ACCGTTTACT TGCCACACCG AGTTTACCGT
    CCTCGACGGG GCTCTGTAGA CTTTAACCCA GAAGAACAGC CGCCGGGTTC TACGCTGCAG
    AGCCGCCTCC AAACTGTGAC TGAAGAGCAG TGCCACCACA ATGGCCCAGA ACCACTCCCA
    CCCTTTATAG GATTCATCAC ATGCAACCGT ATGGAGAGCA GCCCCGACTA TTCCAGTGAT
    TCTTCCCAAA GGAATTCATC CGATCCCGAT TATCTCTCTC CTCCTCTGGC CTTCAGCTCC
    AATATCTCTA TGGCTTCCAA GCAGAAAACG TTGCAGGATC CACAGATTTT CTTTTGTGTC
    CCACAGTCCG GAACGACGGC TTATGCTGGA CCATCAGCTT TGTACAGCCA GCAGCCACAA
    ATATCATCTG GACAACCACC CACCACATCC ATGGGAATCA GTGCTCAGCC CGGTCCCCAC
    ACCCAGGGCA TGGTGCATCA TCAGCCTTCT CTGCATCCAG CCTCGCAGTA CCAAGTGCAG
    CAGCCAATGC CTACTGTGGG TTCCAGTATT GCGGCCCCAT CACAGCCGGT GATTACCCAA
    ACGCCAGCCC CTCAGCCGGG CACCCAGCTC CCTGCCTCCC AGCCTTTGCC TGCTGTTCAA
    GGAGAGCCTC AGATTCCTGC TCAGACAGCT CCTGCTCCCC AGCCCGTTCC TACAGTGCCG
    GCCTCTCTGC CACCTCATTT TCTTCCCTTG GCACAATCTT TGGTTGCAGT CCCTCTCATT
    CCTCCATTCG CTGCCCCACA GTTGCCTTTA ACGGTACCAC CCCCGGTCAT ACAGACAGGG
    TTCCCTTCCC TACCCATGAC TGTAACCCCA AGTATTGGCC AAACTGTACC AGGAATACCC
    CCATCCTTAG GAGCGCCAGT CCTGCCCCAT GGGCAGGCAG TGTTAGTTCC CACTCACATC
    ACTAATGTTG CTCTCCAGCC TTTGGCACCA ATCCCCACAC CTCCTCTGTG CCCTGTAACT
    CAGCCCATTG GATTACCGGC CACCCTGGGA AAAGCTGCTG AGCCCTTGCT TCCTCCGGGA
    GATACTTCTT ACCAGGTTTT CCAGGCTACT CACCCTCTAT TCCCTGGAGA TGTAAGCTTT
    GTTCCTCCCG TTGCCATCCC CTCAGCAATG GTATCTCCCC CTATCCGTGG TGAAAGTTTA
    GCCCAACCTG GCTTCCATAC TCAGCCTTAT CCTGAAAACA GCAATTTGTA TTCCCATGGA
    GGGCCCACTC CCCAGCCCGC TGTTCTCACT ACCCAGCCGG TGCCTGCAGG AACCCAGACG
    CAACCTGCCG TCGAGGGTTC ACAGAGTAGC TCCCTGGCAA TTTCCCAGGA TACCCAGTCA
    TCTCCTCAAG CAACACAAAC TGCCTCCTCC ATGGACAGCG CTCATTCAGA CGTGGCATCA
    GGACTGAGTG ACGGAAACGA GGGCAGCACA GGTGGGAGAC ACGAAGGCCG AGCCACTAAA
    CGACACTACC GAAAATCTGT GAGAAGCCGC TCCAGGCACG AGAAAACTGC ACGCCCAAAG
    CTTCGAATCC TTAATGTTTC CAACAAAGGG GATCGTGTTG TAGAGTGTCA GCTGGAGACT
    CACAACCGCA AGATGGTCAC CTTCAAATTC GACCTGGATG GAGACAATCC AGAGGAGATT
    GCTACTATAA TGGTACAGAA TGTGTTTATT TTGGCTACGG AGAGAGAATC CTTTGTAGAG
    CAGGTAAAAG ACATAATTGA GAAGGCCGAT GAAATGTTGA GCGAAGATGT GAGCGTGGAG
    CCGGAAGGAG AAACGAGGGC AGAAAGTTTG CAAATCAAGG ATGATGGGGA TTACCCAGAA
    TCTCAGAAGA TGAATTCTGA ATTTCGAATG CCAGATCCTG TATATTCCCT ACCACAGACA
    GCAGGGCTTA TTCCCAGCGT TGTCACACAA GTCGTTCATT CTGCCGGAAG ACGATTTATT
    GTCAGTCCGG TGCCTGAAAG CCGTCTAAAA GAACAGTCAT TCTTCAGTAC TTCATTGCAG
    GACCCTCAGC TACCAGTCTC TGTCGCGCAT GGTCCAGGGA TGAATTTGTC TCATTCTGCC
    TCTTCTGTGA GCCTGCAGCA AGCCTTTGCA GAACTACGCC AGTCACAGAT GAGCGAGGGT
    CCTAGCACAG CTCCTCCCAT ATTTAACCAA ACTGGACCCC CCTTTCCACC ATCCATGACT
    AGTCTGGTGG CAACTCTGCC TTCTAATGCA ATGCCTATCC TCCAGGCAGC CCCAGTGATC
    CCCAGTAGCC TGCCATCTTC TGTGCCTCAG CCCCTGGTGG AGGTGGTTGT ACCTCAGGCT
    GCTGGGATGG CTCCAACTAG CAATGTTCCA AGCACCTTGT CCCCTCCCCT AACTGCAGTA
    TCCAACCAGC ACGCAGACAT GATACCGGGT GCTGCTGTAG CAGCTATCGG ATCACTTCCT
    GTGACATCAC TTCCTCCTTT GCCCATTGTG GGTGGAGTTC CTGTAGGCAC TGCAACCCTG
    AGCTCACCCC TAACACCCCT AATCAGTTCT GTCAGTACAA TGGGGGGGAC GGTTCCCCCA
    TCTGTCACAT CTCCCCCAGT GCCACAGATA GCGGGTAGCG TTGCCGGGAG CCTGCCTCCT
    GCATCAGCCT CCGCCCCTCT TATTCATGGC AGTGTTGCCC CATCTCAGGT ACCGCTTTGC
    AGCAGCAGTT CTGTTCCCAG CTTTGCAGAG AGCGTGGCAG TCAGCCCCCT GCTGGATAGT
    GGCCAAAAGG CAGCAGAAGT GGGCACGGTA ATGAGCACGA CTGGCTTGTC TCTGCCCATC
    TCTGCACCTC TGACATATGC TGGGGTGGTT GCAGCATCAG GCATCACAAG TCAACCTGCC
    GTGCATCTCC CCCAGGGGGT GGCCACAACA CCAGTTGTAT CCCAGGCAGT AGGGGGAGCT
    GTGGGCACAC CCCTCTCATC TCTTCCTCCC ATCCCTGGAG GCATGTCTGT TAGCCAACCA
    GGATCAAATA TACCCCCTGT GCAACACACG TTGGCCCATG GCCAACCCCA GCCTACCCCT
    CTCCCCAACC AGCCTCATAC GCACCTTGTA GAGGGTGACA CCGATAACCT GCCCAAACCT
    GGCATAGACG ATATAAAAAC CCTGGAGGAG AAGCTGAGGT CGCTGTTCAG TGAGCATGGC
    AGTGCCGGGG CCCCTCATGG ACTCACCCCA TCAGAAACTC AATTGGGTTT GGATGGGGCC
    CCCCTGCCAG GCCCTTCCAC AGGTAGCACC GCTCAGACCA AGCCTGCAGC ATCCTCTTCT
    CCACCAACCA GCTTACCTTT AGGGCCACCT GGAGTGACGA CAATGACTCA GATCATGACA
    CCTGGACAAG CTGCTACACC TGTCACCTAC GTATCTGCAG CTGGAAGCTC TATAGCCACG
    GCCAAAGCTG CCACCCCACC CTCAAAGCCG CCTCTCAGTC GGGTACCGGT GACCACGGCC
    GGGGCAGAGA CCCCAGCAGG CAGTCTCCCT GGTGAACACC TGCCTCCTCC TTCTCTCCCA
    GGACCTTCAC TAACTCAGTC CCAGCAGCCT CTAGAAGACC TGGATTTCCA GCTGAGAAGG
    ACGTTGAGCC CTGACACTGT CCCTGTGCCT TCTGCACTAA TATTTCCTGC ACACAGCGGA
    GCTTCTACCG GTGTTGCAGC ACCCGTCGGC TCAGCATTGC AACCCTCCGC TTCAGAATTG
    CCAAAAGCCA GCGCTGGAGA GGCAGAGGTG GGCGCGAGTG TCCAGAGGAT GGGCAGGTTT
    CAGGTCTCTT TGGCAGCAGA GAATGTGCTC AAGCAGAGCG AGGAGACCAA ACTGTCCATC
    TCAGACACTT CCTCCACCAC TTCTACATCC TCCTCTTCCT CTGCGTCATG CAGCAGCCCA
    GAGAGCACCC TGACTAAGCC CCAGGCCAAA ACCGCTGCTC CCTTAGACAT AGTGGATGGT
    GTTGGTCCCC ACTTGGTGCA TCTACAGAGC CTATCTCCCA AGCAGTTGGT GAAAGTGGGG
    CGCTTCCAGG TTACCACTGC CACCACGGAC AAAGTGGGGC GCTTTTCTGT GTCCCGGACA
    CAAGATGATA TATCTGCTGG GGTGCTGGAG ACAGACTCCC AGAGGTCCTC CTCCCCACAG
    ACTGAGAAAC ATCTGAATGG ACCCTACTTA CAGCAAGACT CCTCTGCGGC TCTCGGCTAT
    CCGGCCAGGG AGACGCAGGA AGACAGCCTA GGGAGCCCCG TTCTGCACCA CGCTTTGGGG
    TCCAAGCTTA GCCTGCAGAG CCTTAGCAAC TCGTTCAACT CTTCCTACAT GAGCAGCGAT
    AATGATTCTG ACATAGAAGA TGAAGATCTG AAGAAGGAGC TGCGAAGGCT ACGAGACAAG
    CATCTCAAGG AGATTCAGGA ACTGCAGAGT CGACAGAAGC AGGAGATCGA GCTGTTGTAC
    ACGGCTTTGG GAAAAGTGCC CCCTGCTGTC ATTATCCCTC CGGCAGCGCC CCTTTCTGGC
    CGGCGCAGGA GGCATACCAA GGGGAAGGGC AGCAAGTCCA GCCGGGGCAG CTCCCACAAC
    AGCCGCAGCC CTCATTTGTC AGGCGACCTG TCCACGCAGA GCGCTCCGTC GGCATTACCC
    CCCCAGCCGG GCCTTCTGCT CCCCCCAAGC AGTGCGCCCG AACATGCTCA TCTTCTCAAG
    CCCTCCCCTT CCAGCGACAT TCTCTGCTCA GCCTTCACTA GCGACGCTGC CCTCTCTGTG
    CCCAGCCTGT CCACCCAGGG CCAAGGCTGG GCCAAGTTTA ACTGTGAGTC AGAGCGGGTA
    TCCTTCAAAA TGGCTGGCAG GAGGACGCGA TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG
    AAGATGGTGA AAAAAGTCTG TCCTTGCAAC CAGCTCTGTA GGACCAGCAG CACTAACACA
    GTCGCACCAT CAACTGCCCC GGCCCCCCAG CCAAACGTGC CCCCCGTGTC ATCCAGCCGC
    AAGGGAACCT TCACGGACGA TCTACACAAA CTGGTGGACA ACTGGGCCCG AGACGCCATG
    AACCTGACTG GAAAAAAGGC TTGTAAGGCC TCTGGGCAAC CCAGCTATGA GGGACCCGGG
    ATGGCCCGTA AGTTCTCTGC TCCCGGACAG CTGTGTGTCC CCATGAACCC ATCCCTGGGG
    GGCTCCGTCC CTCTCCCAGC AGCTTCGGCG ACCTCCCTGG CTCACTACCC CAAAGGAATG
    TGCCCCCCGC AACAATACGG AATCCCCGGG GCGCTGTTCC AAGCTCCATG GAGTGGCACC
    GGCGTGCCCC CCTCCTCTCA GCCCCTAAGC CAGTTCCCCC CGGCGGTGAC ATCTTCCTTA
    CAGAACTTCG GTATTAGCAG CTTGCAGAAA TCTATCAGCA ACCCCCAAGG ATCCAACCTG
    CGGACTACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754477.1
    CDS328..8679
    Translation

    Target ORF information:

    RefSeq Version XM_031898617.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898617.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCTTGTCGA GGTCGTAGCA TGTCAGTATG TGTTCCCAAC
    CTTTCCTCTG CTCCCCCACT CCTTTCCGTT GTGCCCCCCA GTGCTCCAGG CTCCCCTTCC
    CCATTGCAGT CTGCCCCTGC CTCCCCACTC TGGACATCTC CCGAAGATTC CTTTGCTGAA
    AAGCTTTCCA AGGCTCTAGA GAGTGTTCTG CCCATGCACT CCTCCAGCCC TCGCAAGCAC
    CAACGCCGCT GCAGCCTGCC TACCCTCTAT ACCAGCCCTC CATCGTCCAT GGTCCATTCA
    GCACTCCCAG ACTCTCCGCT TTTTTTCCCA ACCATTCCCA ACCAGCGCCC CATGTCTTTC
    TCTCCGCCGC CTACTGGCTT GTCCCCAAAG GTGCCTGCAA CCCAGCGACG CAAGAGCACC
    TCTTTCTTGG AGTCCCAAGG CCGCCAGGGG CTATCTCAGA TTAGGACACT CGGGCCAGGT
    GAGGAGACCC AGTGCTGGAG GGCAGACTAC ATGGCAGCTC TAGGTAAAGC ATCTGTCCGA
    TCCGAGGATG CTCACAAGCA TCAAAACTAT GAGGGATTGG CGTTCCATGA CGCAAGACAT
    GTTCCGACAG TTCCAAATGA AACTCTCTTA AAGCCTGGGG AAGCATTGTA TCTAGCCAGT
    CTGCCCTCAC AGGAGAGCTA CAGCAGCATG GCTGCCCCTG AGCATATGGA GGGCCACAAG
    CTGACCCAGC TTGGCTCGTT TTACGATTAT CACACTGGGC AAACCTACAT TGCGCGGCCC
    CTGAAGAGCC TAAGACTGGA ATCTGGGTTA AACCAGCCGT CGGCTTTGGG TAGCATCTCC
    AACCCAATCT CCTCCGACCC ACACGCCATG TTTCATCAGA TGTTTGTGCC CCAGTCTGCT
    CCTCCAGTGC TGGCTCAAAG CAGCGAAGGC CACCCAGGGT GTGTGTTTGA GTTCCACGTG
    CACCCATCTG GGTCTGTTCC CGGCGAAGCA ACCGTTTACT TGCCACACCG AGTTTACCGT
    CCTCGACGGG GCTCTGTAGA CTTTAACCCA GAAGAACAGC CGCCGGGTTC TACGCTGCAG
    AGCCGCCTCC AAACTGTGAC TGAAGAGCAG TGCCACCACA ATGGCCCAGA ACCACTCCCA
    CCCTTTATAG GATTCATCAC ATGCAACCGT ATGGAGAGCA GCCCCGACTA TTCCAGTGAT
    TCTTCCCAAA GGAATTCATC CGATCCCGAT TATCTCTCTC CTCCTCTGGC CTTCAGCTCC
    AATATCTCTA TGGCTTCCAA GCAGAAAACG TTGCAGGATC CACAGATTTT CTTTTGTGTC
    CCACAGTCCG GAACGACGGC TTATGCTGGA CCATCAGCTT TGTACAGCCA GCAGCCACAA
    ATATCATCTG GACAACCACC CACCACATCC ATGGGAATCA GTGCTCAGCC CGGTCCCCAC
    ACCCAGGGCA TGGTGCATCA TCAGCCTTCT CTGCATCCAG CCTCGCAGTA CCAAGTGCAG
    CAGCCAATGC CTACTGTGGG TTCCAGTATT GCGGCCCCAT CACAGCCGGT GATTACCCAA
    ACGCCAGCCC CTCAGCCGGG CACCCAGCTC CCTGCCTCCC AGCCTTTGCC TGCTGTTCAA
    GGAGAGCCTC AGATTCCTGC TCAGACAGCT CCTGCTCCCC AGCCCGTTCC TACAGTGCCG
    GCCTCTCTGC CACCTCATTT TCTTCCCTTG GCACAATCTT TGGTTGCAGT CCCTCTCATT
    CCTCCATTCG CTGCCCCACA GTTGCCTTTA ACGGTACCAC CCCCGGTCAT ACAGACAGGG
    TTCCCTTCCC TACCCATGAC TGTAACCCCA AGTATTGGCC AAACTGTACC AGGAATACCC
    CCATCCTTAG GAGCGCCAGT CCTGCCCCAT GGGCAGGCAG TGTTAGTTCC CACTCACATC
    ACTAATGTTG CTCTCCAGCC TTTGGCACCA ATCCCCACAC CTCCTCTGTG CCCTGTAACT
    CAGCCCATTG GATTACCGGC CACCCTGGGA AAAGCTGCTG AGCCCTTGCT TCCTCCGGGA
    GATACTTCTT ACCAGGTTTT CCAGGCTACT CACCCTCTAT TCCCTGGAGA TGTAAGCTTT
    GTTCCTCCCG TTGCCATCCC CTCAGCAATG GTATCTCCCC CTATCCGTGG TGAAAGTTTA
    GCCCAACCTG GCTTCCATAC TCAGCCTTAT CCTGAAAACA GCAATTTGTA TTCCCATGGA
    GGGCCCACTC CCCAGCCCGC TGTTCTCACT ACCCAGCCGG TGCCTGCAGG AACCCAGACG
    CAACCTGCCG TCGAGGGTTC ACAGAGTAGC TCCCTGGCAA TTTCCCAGGA TACCCAGTCA
    TCTCCTCAAG CAACACAAAC TGCCTCCTCC ATGGACAGCG CTCATTCAGA CGTGGCATCA
    GGACTGAGTG ACGGAAACGA GGGCAGCACA GGTGGGAGAC ACGAAGGCCG AGCCACTAAA
    CGACACTACC GAAAATCTGT GAGAAGCCGC TCCAGGCACG AGAAAACTGC ACGCCCAAAG
    CTTCGAATCC TTAATGTTTC CAACAAAGGG GATCGTGTTG TAGAGTGTCA GCTGGAGACT
    CACAACCGCA AGATGGTCAC CTTCAAATTC GACCTGGATG GAGACAATCC AGAGGAGATT
    GCTACTATAA TGGTACAGAA TGTGTTTATT TTGGCTACGG AGAGAGAATC CTTTGTAGAG
    CAGGTAAAAG ACATAATTGA GAAGGCCGAT GAAATGTTGA GCGAAGATGT GAGCGTGGAG
    CCGGAAGGAG AAACGAGGGC AGAAAGTTTG CAAATCAAGG ATGATGGGGA TTACCCAGAA
    TCTCAGAAGA TGAATTCTGA ATTTCGAATG CCAGATCCTG TATATTCCCT ACCACAGACA
    GCAGGGCTTA TTCCCAGCGT TGTCACACAA GTCGTTCATT CTGCCGGAAG ACGATTTATT
    GTCAGTCCGG TGCCTGAAAG CCGTCTAAAA GAACAGTCAT TCTTCAGTAC TTCATTGCAG
    GACCCTCAGC TACCAGTCTC TGTCGCGCAT GGTCCAGGGA TGAATTTGTC TCATTCTGCC
    TCTTCTGTGA GCCTGCAGCA AGCCTTTGCA GAACTACGCC AGTCACAGAT GAGCGAGGGT
    CCTAGCACAG CTCCTCCCAT ATTTAACCAA ACTGGACCCC CCTTTCCACC ATCCATGACT
    AGTCTGGTGG CAACTCTGCC TTCTAATGCA ATGCCTATCC TCCAGGCAGC CCCAGTGATC
    CCCAGTAGCC TGCCATCTTC TGTGCCTCAG CCCCTGGTGG AGGTGGTTGT ACCTCAGGCT
    GCTGGGATGG CTCCAACTAG CAATGTTCCA AGCACCTTGT CCCCTCCCCT AACTGCAGTA
    TCCAACCAGC ACGCAGACAT GATACCGGGT GCTGCTGTAG CAGCTATCGG ATCACTTCCT
    GTGACATCAC TTCCTCCTTT GCCCATTGTG GGTGGAGTTC CTGTAGGCAC TGCAACCCTG
    AGCTCACCCC TAACACCCCT AATCAGTTCT GTCAGTACAA TGGGGGGGAC GGTTCCCCCA
    TCTGTCACAT CTCCCCCAGT GCCACAGATA GCGGGTAGCG TTGCCGGGAG CCTGCCTCCT
    GCATCAGCCT CCGCCCCTCT TATTCATGGC AGTGTTGCCC CATCTCAGGT ACCGCTTTGC
    AGCAGCAGTT CTGTTCCCAG CTTTGCAGAG AGCGTGGCAG TCAGCCCCCT GCTGGATAGT
    GGCCAAAAGG CAGCAGAAGT GGGCACGGTA ATGAGCACGA CTGGCTTGTC TCTGCCCATC
    TCTGCACCTC TGACATATGC TGGGGTGGTT GCAGCATCAG GCATCACAAG TCAACCTGCC
    GTGCATCTCC CCCAGGGGGT GGCCACAACA CCAGTTGTAT CCCAGGCAGT AGGGGGAGCT
    GTGGGCACAC CCCTCTCATC TCTTCCTCCC ATCCCTGGAG GCATGTCTGT TAGCCAACCA
    GGATCAAATA TACCCCCTGT GCAACACACG TTGGCCCATG GCCAACCCCA GCCTACCCCT
    CTCCCCAACC AGCCTCATAC GCACCTTGTA GAGGGTGACA CCGATAACCT GCCCAAACCT
    GGCATAGACG ATATAAAAAC CCTGGAGGAG AAGCTGAGGT CGCTGTTCAG TGAGCATGGC
    AGTGCCGGGG CCCCTCATGG ACTCACCCCA TCAGAAACTC AATTGGGTTT GGATGGGGCC
    CCCCTGCCAG GCCCTTCCAC AGGTAGCACC GCTCAGACCA AGCCTGCAGC ATCCTCTTCT
    CCACCAACCA GCTTACCTTT AGGGCCACCT GGAGTGACGA CAATGACTCA GATCATGACA
    CCTGGACAAG CTGCTACACC TGTCACCTAC GTATCTGCAG CTGGAAGCTC TATAGCCACG
    GCCAAAGCTG CCACCCCACC CTCAAAGCCG CCTCTCAGTC GGGTACCGGT GACCACGGCC
    GGGGCAGAGA CCCCAGCAGG CAGTCTCCCT GGTGAACACC TGCCTCCTCC TTCTCTCCCA
    GGACCTTCAC TAACTCAGTC CCAGCAGCCT CTAGAAGACC TGGATTTCCA GCTGAGAAGG
    ACGTTGAGCC CTGACACTGT CCCTGTGCCT TCTGCACTAA TATTTCCTGC ACACAGCGGA
    GCTTCTACCG GTGTTGCAGC ACCCGTCGGC TCAGCATTGC AACCCTCCGC TTCAGAATTG
    CCAAAAGCCA GCGCTGGAGA GGCAGAGGTG GGCGCGAGTG TCCAGAGGAT GGGCAGGTTT
    CAGGTCTCTT TGGCAGCAGA GAATGTGCTC AAGCAGAGCG AGGAGACCAA ACTGTCCATC
    TCAGACACTT CCTCCACCAC TTCTACATCC TCCTCTTCCT CTGCGTCATG CAGCAGCCCA
    GAGAGCACCC TGACTAAGCC CCAGGCCAAA ACCGCTGCTC CCTTAGACAT AGTGGATGGT
    GTTGGTCCCC ACTTGGTGCA TCTACAGAGC CTATCTCCCA AGCAGTTGGT GAAAGTGGGG
    CGCTTCCAGG TTACCACTGC CACCACGGAC AAAGTGGGGC GCTTTTCTGT GTCCCGGACA
    CAAGATGATA TATCTGCTGG GGTGCTGGAG ACAGACTCCC AGAGGTCCTC CTCCCCACAG
    ACTGAGAAAC ATCTGAATGG ACCCTACTTA CAGCAAGACT CCTCTGCGGC TCTCGGCTAT
    CCGGCCAGGG AGACGCAGGA AGACAGCCTA GGGAGCCCCG TTCTGCACCA CGCTTTGGGG
    TCCAAGCTTA GCCTGCAGAG CCTTAGCAAC TCGTTCAACT CTTCCTACAT GAGCAGCGAT
    AATGATTCTG ACATAGAAGA TGAAGATCTG AAGAAGGAGC TGCGAAGGCT ACGAGACAAG
    CATCTCAAGG AGATTCAGGA ACTGCAGAGT CGACAGAAGC AGGAGATCGA GCTGTTGTAC
    ACGGCTTTGG GAAAAGTGCC CCCTGCTGTC ATTATCCCTC CGGCAGCGCC CCTTTCTGGC
    CGGCGCAGGA GGCATACCAA GGGGAAGGGC AGCAAGTCCA GCCGGGGCAG CTCCCACAAC
    AGCCGCAGCC CTCATTTGTC AGGCGACCTG TCCACGCAGA GCGCTCCGTC GGCATTACCC
    CCCCAGCCGG GCCTTCTGCT CCCCCCAAGC AGTGCGCCCG AACATGCTCA TCTTCTCAAG
    CCCTCCCCTT CCAGCGACAT TCTCTGCTCA GCCTTCACTA GCGACGCTGC CCTCTCTGTG
    CCCAGCCTGT CCACCCAGGG CCAAGGCTGG GCCAAGTTTA ACTGTGAGTC AGAGCGGGTA
    TCCTTCAAAA TGGCTGGCAG GAGGACGCGA TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG
    AAGATGGTGA AAAAAGTCTG TCCTTGCAAC CAGCTCTGTA GGACCAGCAG CACTAACACA
    GTCGCACCAT CAACTGCCCC GGCCCCCCAG CCAAACGTGC CCCCCGTGTC ATCCAGCCGC
    AAGGGAACCT TCACGGACGA TCTACACAAA CTGGTGGACA ACTGGGCCCG AGACGCCATG
    AACCTGACTG GAAAAAAGGC TTGTAAGGCC TCTGGGCAAC CCAGCTATGA GGGACCCGGG
    ATGGCCCGTA AGTTCTCTGC TCCCGGACAG CTGTGTGTCC CCATGAACCC ATCCCTGGGG
    GGCTCCGTCC CTCTCCCAGC AGCTTCGGCG ACCTCCCTGG CTCACTACCC CAAAGGAATG
    TGCCCCCCGC AACAATACGG AATCCCCGGG GCGCTGTTCC AAGCTCCATG GAGTGGCACC
    GGCGTGCCCC CCTCCTCTCA GCCCCTAAGC CAGTTCCCCC CGGCGGTGAC ATCTTCCTTA
    CAGAACTTCG GTATTAGCAG CTTGCAGAAA TCTATCAGCA ACCCCCAAGG ATCCAACCTG
    CGGACTACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54259
    Clone ID Related Accession (Same CDS sequence) XM_031898618.1
    Accession Version XM_031898618.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGGTGACC
    ACGGCCGGGG CAGAGACCCC AGCAGGCAGT CTCCCTGGTG AACACCTGCC TCCTCCTTCT
    CTCCCAGGAC CTTCACTAAC TCAGTCCCAG CAGCCTCTAG AAGACCTGGA TTTCCAGCTG
    AGAAGGACGT TGAGCCCTGA CACTGTCCCT GTGCCTTCTG CACTAATATT TCCTGCACAC
    AGCGGAGCTT CTACCGGTGT TGCAGCACCC GTCGGCTCAG CATTGCAACC CTCCGCTTCA
    GAATTGCCAA AAGCCAGCGC TGGAGAGGCA GAGGTGGGCG CGAGTGTCCA GAGGATGGGC
    AGGTTTCAGG TCTCTTTGGC AGCAGAGAAT GTGCTCAAGC AGAGCGAGGA GACCAAACTG
    TCCATCTCAG ACACTTCCTC CACCACTTCT ACATCCTCCT CTTCCTCTGC GTCATGCAGC
    AGCCCAGAGA GCACCCTGAC TAAGCCCCAG GCCAAAACCG CTGCTCCCTT AGACATAGTG
    GATGGTGTTG GTCCCCACTT GGTGCATCTA CAGAGCCTAT CTCCCAAGCA GTTGGTGAAA
    GTGGGGCGCT TCCAGGTTAC CACTGCCACC ACGGACAAAG TGGGGCGCTT TTCTGTGTCC
    CGGACACAAG ATGATATATC TGCTGGGGTG CTGGAGACAG ACTCCCAGAG GTCCTCCTCC
    CCACAGACTG AGAAACATCT GAATGGACCC TACTTACAGC AAGACTCCTC TGCGGCTCTC
    GGCTATCCGG CCAGGGAGAC GCAGGAAGAC AGCCTAGGGA GCCCCGTTCT GCACCACGCT
    TTGGGGTCCA AGCTTAGCCT GCAGAGCCTT AGCAACTCGT TCAACTCTTC CTACATGAGC
    AGCGATAATG ATTCTGACAT AGAAGATGAA GATCTGAAGA AGGAGCTGCG AAGGCTACGA
    GACAAGCATC TCAAGGAGAT TCAGGAACTG CAGAGTCGAC AGAAGCAGGA GATCGAGCTG
    TTGTACACGG CTTTGGGAAA AGTGCCCCCT GCTGTCATTA TCCCTCCGGC AGCGCCCCTT
    TCTGGCCGGC GCAGGAGGCA TACCAAGGGG AAGGGCAGCA AGTCCAGCCG GGGCAGCTCC
    CACAACAGCC GCAGCCCTCA TTTGTCAGGC GACCTGTCCA CGCAGAGCGC TCCGTCGGCA
    TTACCCCCCC AGCCGGGCCT TCTGCTCCCC CCAAGCAGTG CGCCCGAACA TGCTCATCTT
    CTCAAGCCCT CCCCTTCCAG CGACATTCTC TGCTCAGCCT TCACTAGCGA CGCTGCCCTC
    TCTGTGCCCA GCCTGTCCAC CCAGGGCCAA GGCTGGGCCA AGTTTAACTG TGAGTCAGAG
    CGGGTATCCT TCAAAATGGC TGGCAGGAGG ACGCGATTTC TGAGGAAGAT GGTGAAAAAA
    GTCTGTCCTT GCAACCAGCT CTGTAGGACC AGCAGCACTA ACACAGTCGC ACCATCAACT
    GCCCCGGCCC CCCAGCCAAA CGTGCCCCCC GTGTCATCCA GCCGCAAGGG AACCTTCACG
    GACGATCTAC ACAAACTGGT GGACAACTGG GCCCGAGACG CCATGAACCT GACTGGAAAA
    AAGGCTTGTA AGGCCTCTGG GCAACCCAGC TATGAGGGAC CCGGGATGGC CCGTAAGTTC
    TCTGCTCCCG GACAGCTGTG TGTCCCCATG AACCCATCCC TGGGGGGCTC CGTCCCTCTC
    CCAGCAGCTT CGGCGACCTC CCTGGCTCAC TACCCCAAAG GAATGTGCCC CCCGCAACAA
    TACGGAATCC CCGGGGCGCT GTTCCAAGCT CCATGGAGTG GCACCGGCGT GCCCCCCTCC
    TCTCAGCCCC TAAGCCAGTT CCCCCCGGCG GTGACATCTT CCTTACAGAA CTTCGGTATT
    AGCAGCTTGC AGAAATCTAT CAGCAACCCC CAAGGATCCA ACCTGCGGAC TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754478.1
    CDS328..8664
    Translation

    Target ORF information:

    RefSeq Version XM_031898618.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898618.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGGTGACC
    ACGGCCGGGG CAGAGACCCC AGCAGGCAGT CTCCCTGGTG AACACCTGCC TCCTCCTTCT
    CTCCCAGGAC CTTCACTAAC TCAGTCCCAG CAGCCTCTAG AAGACCTGGA TTTCCAGCTG
    AGAAGGACGT TGAGCCCTGA CACTGTCCCT GTGCCTTCTG CACTAATATT TCCTGCACAC
    AGCGGAGCTT CTACCGGTGT TGCAGCACCC GTCGGCTCAG CATTGCAACC CTCCGCTTCA
    GAATTGCCAA AAGCCAGCGC TGGAGAGGCA GAGGTGGGCG CGAGTGTCCA GAGGATGGGC
    AGGTTTCAGG TCTCTTTGGC AGCAGAGAAT GTGCTCAAGC AGAGCGAGGA GACCAAACTG
    TCCATCTCAG ACACTTCCTC CACCACTTCT ACATCCTCCT CTTCCTCTGC GTCATGCAGC
    AGCCCAGAGA GCACCCTGAC TAAGCCCCAG GCCAAAACCG CTGCTCCCTT AGACATAGTG
    GATGGTGTTG GTCCCCACTT GGTGCATCTA CAGAGCCTAT CTCCCAAGCA GTTGGTGAAA
    GTGGGGCGCT TCCAGGTTAC CACTGCCACC ACGGACAAAG TGGGGCGCTT TTCTGTGTCC
    CGGACACAAG ATGATATATC TGCTGGGGTG CTGGAGACAG ACTCCCAGAG GTCCTCCTCC
    CCACAGACTG AGAAACATCT GAATGGACCC TACTTACAGC AAGACTCCTC TGCGGCTCTC
    GGCTATCCGG CCAGGGAGAC GCAGGAAGAC AGCCTAGGGA GCCCCGTTCT GCACCACGCT
    TTGGGGTCCA AGCTTAGCCT GCAGAGCCTT AGCAACTCGT TCAACTCTTC CTACATGAGC
    AGCGATAATG ATTCTGACAT AGAAGATGAA GATCTGAAGA AGGAGCTGCG AAGGCTACGA
    GACAAGCATC TCAAGGAGAT TCAGGAACTG CAGAGTCGAC AGAAGCAGGA GATCGAGCTG
    TTGTACACGG CTTTGGGAAA AGTGCCCCCT GCTGTCATTA TCCCTCCGGC AGCGCCCCTT
    TCTGGCCGGC GCAGGAGGCA TACCAAGGGG AAGGGCAGCA AGTCCAGCCG GGGCAGCTCC
    CACAACAGCC GCAGCCCTCA TTTGTCAGGC GACCTGTCCA CGCAGAGCGC TCCGTCGGCA
    TTACCCCCCC AGCCGGGCCT TCTGCTCCCC CCAAGCAGTG CGCCCGAACA TGCTCATCTT
    CTCAAGCCCT CCCCTTCCAG CGACATTCTC TGCTCAGCCT TCACTAGCGA CGCTGCCCTC
    TCTGTGCCCA GCCTGTCCAC CCAGGGCCAA GGCTGGGCCA AGTTTAACTG TGAGTCAGAG
    CGGGTATCCT TCAAAATGGC TGGCAGGAGG ACGCGATTTC TGAGGAAGAT GGTGAAAAAA
    GTCTGTCCTT GCAACCAGCT CTGTAGGACC AGCAGCACTA ACACAGTCGC ACCATCAACT
    GCCCCGGCCC CCCAGCCAAA CGTGCCCCCC GTGTCATCCA GCCGCAAGGG AACCTTCACG
    GACGATCTAC ACAAACTGGT GGACAACTGG GCCCGAGACG CCATGAACCT GACTGGAAAA
    AAGGCTTGTA AGGCCTCTGG GCAACCCAGC TATGAGGGAC CCGGGATGGC CCGTAAGTTC
    TCTGCTCCCG GACAGCTGTG TGTCCCCATG AACCCATCCC TGGGGGGCTC CGTCCCTCTC
    CCAGCAGCTT CGGCGACCTC CCTGGCTCAC TACCCCAAAG GAATGTGCCC CCCGCAACAA
    TACGGAATCC CCGGGGCGCT GTTCCAAGCT CCATGGAGTG GCACCGGCGT GCCCCCCTCC
    TCTCAGCCCC TAAGCCAGTT CCCCCCGGCG GTGACATCTT CCTTACAGAA CTTCGGTATT
    AGCAGCTTGC AGAAATCTAT CAGCAACCCC CAAGGATCCA ACCTGCGGAC TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54260
    Clone ID Related Accession (Same CDS sequence) XM_031898619.1
    Accession Version XM_031898619.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGGTGACC
    ACGGCCGGGG CAGAGACCCC AGCAGGCAGT CTCCCTGGTG AACACCTGCC TCCTCCTTCT
    CTCCCAGGAC CTTCACTAAC TCAGTCCCAG CAGCCTCTAG AAGACCTGGA TTTCCAGCTG
    AGAAGGACGT TGAGCCCTGA CACTGTCCCT GTGCCTTCTG CACTAATATT TCCTGCACAC
    AGCGGAGCTT CTACCGGTGT TGCAGCACCC GTCGGCTCAG CATTGCAACC CTCCGCTTCA
    GAATTGCCAA AAGCCAGCGC TGGAGAGGCA GAGGTGGGCG CGAGTGTCCA GAGGATGGGC
    AGGTTTCAGG TCTCTTTGGC AGCAGAGAAT GTGCTCAAGC AGAGCGAGGA GACCAAACTG
    TCCATCTCAG ACACTTCCTC CACCACTTCT ACATCCTCCT CTTCCTCTGC GTCATGCAGC
    AGCCCAGAGA GCACCCTGAC TAAGCCCCAG GCCAAAACCG CTGCTCCCTT AGACATAGTG
    GATGGTGTTG GTCCCCACTT GGTGCATCTA CAGAGCCTAT CTCCCAAGCA GTTGGTGAAA
    GTGGGGCGCT TCCAGGTTAC CACTGCCACC ACGGACAAAG TGGGGCGCTT TTCTGTGTCC
    CGGACACAAG ATGATATATC TGCTGGGGTG CTGGAGACAG ACTCCCAGAG GTCCTCCTCC
    CCACAGACTG AGAAACATCT GAATGGACCC TACTTACAGC AAGACTCCTC TGCGGCTCTC
    GGCTATCCGG CCAGGGAGAC GCAGGAAGAC AGCCTAGGGA GCCCCGTTCT GCACCACGCT
    TTGGGGTCCA AGCTTAGCCT GCAGAGCCTT AGCAACTCGT TCAACTCTTC CTACATGAGC
    AGCGATAATG ATTCTGACAT AGAAGATGAA GATCTGAAGA AGGAGCTGCG AAGGCTACGA
    GACAAGCATC TCAAGGAGAT TCAGGAACTG CAGAGTCGAC AGAAGCAGGA GATCGAGCTG
    TTGTACACGG CTTTGGGAAA AGTGCCCCCT GCTGTCATTA TCCCTCCGGC AGCGCCCCTT
    TCTGGCCGGC GCAGGAGGCA TACCAAGGGG AAGGGCAGCA AGTCCAGCCG GGGCAGCTCC
    CACAACAGCC GCAGCCCTCA TTTGTCAGGC GACCTGTCCA CGCAGAGCGC TCCGTCGGCA
    TTACCCCCCC AGCCGGGCCT TCTGCTCCCC CCAAGCAGTG CGCCCGAACA TGCTCATCTT
    CTCAAGCCCT CCCCTTCCAG CGACATTCTC TGCTCAGCCT TCACTAGCGA CGCTGCCCTC
    TCTGTGCCCA GCCTGTCCAC CCAGGGCCAA GGCTGGGCCA AGTTTAACTG TGAGTCAGAG
    CGGGTATCCT TCAAAATGGC TGGCAGGAGG ACGCGATTTC TGAGGACCAG CAGCACTAAC
    ACAGTCGCAC CATCAACTGC CCCGGCCCCC CAGCCAAACG TGCCCCCCGT GTCATCCAGC
    CGCAAGGGAA CCTTCACGGA CGATCTACAC AAACTGGTGG ACAACTGGGC CCGAGACGCC
    ATGAACCTGA CTGGAAAAAA GGCTTGTAAG GCCTCTGGGC AACCCAGCTA TGAGGGACCC
    GGGATGGCCC GTAAGTTCTC TGCTCCCGGA CAGCTGTGTG TCCCCATGAA CCCATCCCTG
    GGGGGCTCCG TCCCTCTCCC AGCAGCTTCG GCGACCTCCC TGGCTCACTA CCCCAAAGGA
    ATGTGCCCCC CGCAACAATA CGGAATCCCC GGGGCGCTGT TCCAAGCTCC ATGGAGTGGC
    ACCGGCGTGC CCCCCTCCTC TCAGCCCCTA AGCCAGTTCC CCCCGGCGGT GACATCTTCC
    TTACAGAACT TCGGTATTAG CAGCTTGCAG AAATCTATCA GCAACCCCCA AGGATCCAAC
    CTGCGGACTA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754479.1
    CDS328..8622
    Translation

    Target ORF information:

    RefSeq Version XM_031898619.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898619.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGGTGACC
    ACGGCCGGGG CAGAGACCCC AGCAGGCAGT CTCCCTGGTG AACACCTGCC TCCTCCTTCT
    CTCCCAGGAC CTTCACTAAC TCAGTCCCAG CAGCCTCTAG AAGACCTGGA TTTCCAGCTG
    AGAAGGACGT TGAGCCCTGA CACTGTCCCT GTGCCTTCTG CACTAATATT TCCTGCACAC
    AGCGGAGCTT CTACCGGTGT TGCAGCACCC GTCGGCTCAG CATTGCAACC CTCCGCTTCA
    GAATTGCCAA AAGCCAGCGC TGGAGAGGCA GAGGTGGGCG CGAGTGTCCA GAGGATGGGC
    AGGTTTCAGG TCTCTTTGGC AGCAGAGAAT GTGCTCAAGC AGAGCGAGGA GACCAAACTG
    TCCATCTCAG ACACTTCCTC CACCACTTCT ACATCCTCCT CTTCCTCTGC GTCATGCAGC
    AGCCCAGAGA GCACCCTGAC TAAGCCCCAG GCCAAAACCG CTGCTCCCTT AGACATAGTG
    GATGGTGTTG GTCCCCACTT GGTGCATCTA CAGAGCCTAT CTCCCAAGCA GTTGGTGAAA
    GTGGGGCGCT TCCAGGTTAC CACTGCCACC ACGGACAAAG TGGGGCGCTT TTCTGTGTCC
    CGGACACAAG ATGATATATC TGCTGGGGTG CTGGAGACAG ACTCCCAGAG GTCCTCCTCC
    CCACAGACTG AGAAACATCT GAATGGACCC TACTTACAGC AAGACTCCTC TGCGGCTCTC
    GGCTATCCGG CCAGGGAGAC GCAGGAAGAC AGCCTAGGGA GCCCCGTTCT GCACCACGCT
    TTGGGGTCCA AGCTTAGCCT GCAGAGCCTT AGCAACTCGT TCAACTCTTC CTACATGAGC
    AGCGATAATG ATTCTGACAT AGAAGATGAA GATCTGAAGA AGGAGCTGCG AAGGCTACGA
    GACAAGCATC TCAAGGAGAT TCAGGAACTG CAGAGTCGAC AGAAGCAGGA GATCGAGCTG
    TTGTACACGG CTTTGGGAAA AGTGCCCCCT GCTGTCATTA TCCCTCCGGC AGCGCCCCTT
    TCTGGCCGGC GCAGGAGGCA TACCAAGGGG AAGGGCAGCA AGTCCAGCCG GGGCAGCTCC
    CACAACAGCC GCAGCCCTCA TTTGTCAGGC GACCTGTCCA CGCAGAGCGC TCCGTCGGCA
    TTACCCCCCC AGCCGGGCCT TCTGCTCCCC CCAAGCAGTG CGCCCGAACA TGCTCATCTT
    CTCAAGCCCT CCCCTTCCAG CGACATTCTC TGCTCAGCCT TCACTAGCGA CGCTGCCCTC
    TCTGTGCCCA GCCTGTCCAC CCAGGGCCAA GGCTGGGCCA AGTTTAACTG TGAGTCAGAG
    CGGGTATCCT TCAAAATGGC TGGCAGGAGG ACGCGATTTC TGAGGACCAG CAGCACTAAC
    ACAGTCGCAC CATCAACTGC CCCGGCCCCC CAGCCAAACG TGCCCCCCGT GTCATCCAGC
    CGCAAGGGAA CCTTCACGGA CGATCTACAC AAACTGGTGG ACAACTGGGC CCGAGACGCC
    ATGAACCTGA CTGGAAAAAA GGCTTGTAAG GCCTCTGGGC AACCCAGCTA TGAGGGACCC
    GGGATGGCCC GTAAGTTCTC TGCTCCCGGA CAGCTGTGTG TCCCCATGAA CCCATCCCTG
    GGGGGCTCCG TCCCTCTCCC AGCAGCTTCG GCGACCTCCC TGGCTCACTA CCCCAAAGGA
    ATGTGCCCCC CGCAACAATA CGGAATCCCC GGGGCGCTGT TCCAAGCTCC ATGGAGTGGC
    ACCGGCGTGC CCCCCTCCTC TCAGCCCCTA AGCCAGTTCC CCCCGGCGGT GACATCTTCC
    TTACAGAACT TCGGTATTAG CAGCTTGCAG AAATCTATCA GCAACCCCCA AGGATCCAAC
    CTGCGGACTA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54261
    Clone ID Related Accession (Same CDS sequence) XM_031898620.1
    Accession Version XM_031898620.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGGTGACC
    ACGGCCGGGG CAGAGACCCC AGCAGGCAGT CTCCCTGGTG AACACCTGCC TCCTCCTTCT
    CTCCCAGGAC CTTCACTAAC TCAGCCTGCA CACAGCGGAG CTTCTACCGG TGTTGCAGCA
    CCCGTCGGCT CAGCATTGCA ACCCTCCGCT TCAGAATTGC CAAAAGCCAG CGCTGGAGAG
    GCAGAGGTGG GCGCGAGTGT CCAGAGGATG GGCAGGTTTC AGGTCTCTTT GGCAGCAGAG
    AATGTGCTCA AGCAGAGCGA GGAGACCAAA CTGTCCATCT CAGACACTTC CTCCACCACT
    TCTACATCCT CCTCTTCCTC TGCGTCATGC AGCAGCCCAG AGAGCACCCT GACTAAGCCC
    CAGGCCAAAA CCGCTGCTCC CTTAGACATA GTGGATGGTG TTGGTCCCCA CTTGGTGCAT
    CTACAGAGCC TATCTCCCAA GCAGTTGGTG AAAGTGGGGC GCTTCCAGGT TACCACTGCC
    ACCACGGACA AAGTGGGGCG CTTTTCTGTG TCCCGGACAC AAGATGATAT ATCTGCTGGG
    GTGCTGGAGA CAGACTCCCA GAGGTCCTCC TCCCCACAGA CTGAGAAACA TCTGAATGGA
    CCCTACTTAC AGCAAGACTC CTCTGCGGCT CTCGGCTATC CGGCCAGGGA GACGCAGGAA
    GACAGCCTAG GGAGCCCCGT TCTGCACCAC GCTTTGGGGT CCAAGCTTAG CCTGCAGAGC
    CTTAGCAACT CGTTCAACTC TTCCTACATG AGCAGCGATA ATGATTCTGA CATAGAAGAT
    GAAGATCTGA AGAAGGAGCT GCGAAGGCTA CGAGACAAGC ATCTCAAGGA GATTCAGGAA
    CTGCAGAGTC GACAGAAGCA GGAGATCGAG CTGTTGTACA CGGCTTTGGG AAAAGTGCCC
    CCTGCTGTCA TTATCCCTCC GGCAGCGCCC CTTTCTGGCC GGCGCAGGAG GCATACCAAG
    GGGAAGGGCA GCAAGTCCAG CCGGGGCAGC TCCCACAACA GCCGCAGCCC TCATTTGTCA
    GGCGACCTGT CCACGCAGAG CGCTCCGTCG GCATTACCCC CCCAGCCGGG CCTTCTGCTC
    CCCCCAAGCA GTGCGCCCGA ACATGCTCAT CTTCTCAAGC CCTCCCCTTC CAGCGACATT
    CTCTGCTCAG CCTTCACTAG CGACGCTGCC CTCTCTGTGC CCAGCCTGTC CACCCAGGGC
    CAAGGCTGGG CCAAGTTTAA CTGTGAGTCA GAGCGGGTAT CCTTCAAAAT GGCTGGCAGG
    AGGACGCGAT TTCTGAGTAC GCCCTGCTTG GCTCTTTGGA AGATGGTGAA AAAAGTCTGT
    CCTTGCAACC AGCTCTGTAG GACCAGCAGC ACTAACACAG TCGCACCATC AACTGCCCCG
    GCCCCCCAGC CAAACGTGCC CCCCGTGTCA TCCAGCCGCA AGGGAACCTT CACGGACGAT
    CTACACAAAC TGGTGGACAA CTGGGCCCGA GACGCCATGA ACCTGACTGG AAAAAAGGCT
    TGTAAGGCCT CTGGGCAACC CAGCTATGAG GGACCCGGGA TGGCCCGTAA GTTCTCTGCT
    CCCGGACAGC TGTGTGTCCC CATGAACCCA TCCCTGGGGG GCTCCGTCCC TCTCCCAGCA
    GCTTCGGCGA CCTCCCTGGC TCACTACCCC AAAGGAATGT GCCCCCCGCA ACAATACGGA
    ATCCCCGGGG CGCTGTTCCA AGCTCCATGG AGTGGCACCG GCGTGCCCCC CTCCTCTCAG
    CCCCTAAGCC AGTTCCCCCC GGCGGTGACA TCTTCCTTAC AGAACTTCGG TATTAGCAGC
    TTGCAGAAAT CTATCAGCAA CCCCCAAGGA TCCAACCTGC GGACTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754480.1
    CDS328..8598
    Translation

    Target ORF information:

    RefSeq Version XM_031898620.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898620.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGGTGACC
    ACGGCCGGGG CAGAGACCCC AGCAGGCAGT CTCCCTGGTG AACACCTGCC TCCTCCTTCT
    CTCCCAGGAC CTTCACTAAC TCAGCCTGCA CACAGCGGAG CTTCTACCGG TGTTGCAGCA
    CCCGTCGGCT CAGCATTGCA ACCCTCCGCT TCAGAATTGC CAAAAGCCAG CGCTGGAGAG
    GCAGAGGTGG GCGCGAGTGT CCAGAGGATG GGCAGGTTTC AGGTCTCTTT GGCAGCAGAG
    AATGTGCTCA AGCAGAGCGA GGAGACCAAA CTGTCCATCT CAGACACTTC CTCCACCACT
    TCTACATCCT CCTCTTCCTC TGCGTCATGC AGCAGCCCAG AGAGCACCCT GACTAAGCCC
    CAGGCCAAAA CCGCTGCTCC CTTAGACATA GTGGATGGTG TTGGTCCCCA CTTGGTGCAT
    CTACAGAGCC TATCTCCCAA GCAGTTGGTG AAAGTGGGGC GCTTCCAGGT TACCACTGCC
    ACCACGGACA AAGTGGGGCG CTTTTCTGTG TCCCGGACAC AAGATGATAT ATCTGCTGGG
    GTGCTGGAGA CAGACTCCCA GAGGTCCTCC TCCCCACAGA CTGAGAAACA TCTGAATGGA
    CCCTACTTAC AGCAAGACTC CTCTGCGGCT CTCGGCTATC CGGCCAGGGA GACGCAGGAA
    GACAGCCTAG GGAGCCCCGT TCTGCACCAC GCTTTGGGGT CCAAGCTTAG CCTGCAGAGC
    CTTAGCAACT CGTTCAACTC TTCCTACATG AGCAGCGATA ATGATTCTGA CATAGAAGAT
    GAAGATCTGA AGAAGGAGCT GCGAAGGCTA CGAGACAAGC ATCTCAAGGA GATTCAGGAA
    CTGCAGAGTC GACAGAAGCA GGAGATCGAG CTGTTGTACA CGGCTTTGGG AAAAGTGCCC
    CCTGCTGTCA TTATCCCTCC GGCAGCGCCC CTTTCTGGCC GGCGCAGGAG GCATACCAAG
    GGGAAGGGCA GCAAGTCCAG CCGGGGCAGC TCCCACAACA GCCGCAGCCC TCATTTGTCA
    GGCGACCTGT CCACGCAGAG CGCTCCGTCG GCATTACCCC CCCAGCCGGG CCTTCTGCTC
    CCCCCAAGCA GTGCGCCCGA ACATGCTCAT CTTCTCAAGC CCTCCCCTTC CAGCGACATT
    CTCTGCTCAG CCTTCACTAG CGACGCTGCC CTCTCTGTGC CCAGCCTGTC CACCCAGGGC
    CAAGGCTGGG CCAAGTTTAA CTGTGAGTCA GAGCGGGTAT CCTTCAAAAT GGCTGGCAGG
    AGGACGCGAT TTCTGAGTAC GCCCTGCTTG GCTCTTTGGA AGATGGTGAA AAAAGTCTGT
    CCTTGCAACC AGCTCTGTAG GACCAGCAGC ACTAACACAG TCGCACCATC AACTGCCCCG
    GCCCCCCAGC CAAACGTGCC CCCCGTGTCA TCCAGCCGCA AGGGAACCTT CACGGACGAT
    CTACACAAAC TGGTGGACAA CTGGGCCCGA GACGCCATGA ACCTGACTGG AAAAAAGGCT
    TGTAAGGCCT CTGGGCAACC CAGCTATGAG GGACCCGGGA TGGCCCGTAA GTTCTCTGCT
    CCCGGACAGC TGTGTGTCCC CATGAACCCA TCCCTGGGGG GCTCCGTCCC TCTCCCAGCA
    GCTTCGGCGA CCTCCCTGGC TCACTACCCC AAAGGAATGT GCCCCCCGCA ACAATACGGA
    ATCCCCGGGG CGCTGTTCCA AGCTCCATGG AGTGGCACCG GCGTGCCCCC CTCCTCTCAG
    CCCCTAAGCC AGTTCCCCCC GGCGGTGACA TCTTCCTTAC AGAACTTCGG TATTAGCAGC
    TTGCAGAAAT CTATCAGCAA CCCCCAAGGA TCCAACCTGC GGACTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54262
    Clone ID Related Accession (Same CDS sequence) XM_031898621.1
    Accession Version XM_031898621.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGTCCCAG
    CAGCCTCTAG AAGACCTGGA TTTCCAGCTG AGAAGGACGT TGAGCCCTGA CACTGTCCCT
    GTGCCTTCTG CACTAATATT TCCTGCACAC AGCGGAGCTT CTACCGGTGT TGCAGCACCC
    GTCGGCTCAG CATTGCAACC CTCCGCTTCA GAATTGCCAA AAGCCAGCGC TGGAGAGGCA
    GAGGTGGGCG CGAGTGTCCA GAGGATGGGC AGGTTTCAGG TCTCTTTGGC AGCAGAGAAT
    GTGCTCAAGC AGAGCGAGGA GACCAAACTG TCCATCTCAG ACACTTCCTC CACCACTTCT
    ACATCCTCCT CTTCCTCTGC GTCATGCAGC AGCCCAGAGA GCACCCTGAC TAAGCCCCAG
    GCCAAAACCG CTGCTCCCTT AGACATAGTG GATGGTGTTG GTCCCCACTT GGTGCATCTA
    CAGAGCCTAT CTCCCAAGCA GTTGGTGAAA GTGGGGCGCT TCCAGGTTAC CACTGCCACC
    ACGGACAAAG TGGGGCGCTT TTCTGTGTCC CGGACACAAG ATGATATATC TGCTGGGGTG
    CTGGAGACAG ACTCCCAGAG GTCCTCCTCC CCACAGACTG AGAAACATCT GAATGGACCC
    TACTTACAGC AAGACTCCTC TGCGGCTCTC GGCTATCCGG CCAGGGAGAC GCAGGAAGAC
    AGCCTAGGGA GCCCCGTTCT GCACCACGCT TTGGGGTCCA AGCTTAGCCT GCAGAGCCTT
    AGCAACTCGT TCAACTCTTC CTACATGAGC AGCGATAATG ATTCTGACAT AGAAGATGAA
    GATCTGAAGA AGGAGCTGCG AAGGCTACGA GACAAGCATC TCAAGGAGAT TCAGGAACTG
    CAGAGTCGAC AGAAGCAGGA GATCGAGCTG TTGTACACGG CTTTGGGAAA AGTGCCCCCT
    GCTGTCATTA TCCCTCCGGC AGCGCCCCTT TCTGGCCGGC GCAGGAGGCA TACCAAGGGG
    AAGGGCAGCA AGTCCAGCCG GGGCAGCTCC CACAACAGCC GCAGCCCTCA TTTGTCAGGC
    GACCTGTCCA CGCAGAGCGC TCCGTCGGCA TTACCCCCCC AGCCGGGCCT TCTGCTCCCC
    CCAAGCAGTG CGCCCGAACA TGCTCATCTT CTCAAGCCCT CCCCTTCCAG CGACATTCTC
    TGCTCAGCCT TCACTAGCGA CGCTGCCCTC TCTGTGCCCA GCCTGTCCAC CCAGGGCCAA
    GGCTGGGCCA AGTTTAACTG TGAGTCAGAG CGGGTATCCT TCAAAATGGC TGGCAGGAGG
    ACGCGATTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT
    TGCAACCAGC TCTGTAGGAC CAGCAGCACT AACACAGTCG CACCATCAAC TGCCCCGGCC
    CCCCAGCCAA ACGTGCCCCC CGTGTCATCC AGCCGCAAGG GAACCTTCAC GGACGATCTA
    CACAAACTGG TGGACAACTG GGCCCGAGAC GCCATGAACC TGACTGGAAA AAAGGCTTGT
    AAGGCCTCTG GGCAACCCAG CTATGAGGGA CCCGGGATGG CCCGTAAGTT CTCTGCTCCC
    GGACAGCTGT GTGTCCCCAT GAACCCATCC CTGGGGGGCT CCGTCCCTCT CCCAGCAGCT
    TCGGCGACCT CCCTGGCTCA CTACCCCAAA GGAATGTGCC CCCCGCAACA ATACGGAATC
    CCCGGGGCGC TGTTCCAAGC TCCATGGAGT GGCACCGGCG TGCCCCCCTC CTCTCAGCCC
    CTAAGCCAGT TCCCCCCGGC GGTGACATCT TCCTTACAGA ACTTCGGTAT TAGCAGCTTG
    CAGAAATCTA TCAGCAACCC CCAAGGATCC AACCTGCGGA CTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754481.1
    CDS328..8595
    Translation

    Target ORF information:

    RefSeq Version XM_031898621.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898621.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGTCCCAG
    CAGCCTCTAG AAGACCTGGA TTTCCAGCTG AGAAGGACGT TGAGCCCTGA CACTGTCCCT
    GTGCCTTCTG CACTAATATT TCCTGCACAC AGCGGAGCTT CTACCGGTGT TGCAGCACCC
    GTCGGCTCAG CATTGCAACC CTCCGCTTCA GAATTGCCAA AAGCCAGCGC TGGAGAGGCA
    GAGGTGGGCG CGAGTGTCCA GAGGATGGGC AGGTTTCAGG TCTCTTTGGC AGCAGAGAAT
    GTGCTCAAGC AGAGCGAGGA GACCAAACTG TCCATCTCAG ACACTTCCTC CACCACTTCT
    ACATCCTCCT CTTCCTCTGC GTCATGCAGC AGCCCAGAGA GCACCCTGAC TAAGCCCCAG
    GCCAAAACCG CTGCTCCCTT AGACATAGTG GATGGTGTTG GTCCCCACTT GGTGCATCTA
    CAGAGCCTAT CTCCCAAGCA GTTGGTGAAA GTGGGGCGCT TCCAGGTTAC CACTGCCACC
    ACGGACAAAG TGGGGCGCTT TTCTGTGTCC CGGACACAAG ATGATATATC TGCTGGGGTG
    CTGGAGACAG ACTCCCAGAG GTCCTCCTCC CCACAGACTG AGAAACATCT GAATGGACCC
    TACTTACAGC AAGACTCCTC TGCGGCTCTC GGCTATCCGG CCAGGGAGAC GCAGGAAGAC
    AGCCTAGGGA GCCCCGTTCT GCACCACGCT TTGGGGTCCA AGCTTAGCCT GCAGAGCCTT
    AGCAACTCGT TCAACTCTTC CTACATGAGC AGCGATAATG ATTCTGACAT AGAAGATGAA
    GATCTGAAGA AGGAGCTGCG AAGGCTACGA GACAAGCATC TCAAGGAGAT TCAGGAACTG
    CAGAGTCGAC AGAAGCAGGA GATCGAGCTG TTGTACACGG CTTTGGGAAA AGTGCCCCCT
    GCTGTCATTA TCCCTCCGGC AGCGCCCCTT TCTGGCCGGC GCAGGAGGCA TACCAAGGGG
    AAGGGCAGCA AGTCCAGCCG GGGCAGCTCC CACAACAGCC GCAGCCCTCA TTTGTCAGGC
    GACCTGTCCA CGCAGAGCGC TCCGTCGGCA TTACCCCCCC AGCCGGGCCT TCTGCTCCCC
    CCAAGCAGTG CGCCCGAACA TGCTCATCTT CTCAAGCCCT CCCCTTCCAG CGACATTCTC
    TGCTCAGCCT TCACTAGCGA CGCTGCCCTC TCTGTGCCCA GCCTGTCCAC CCAGGGCCAA
    GGCTGGGCCA AGTTTAACTG TGAGTCAGAG CGGGTATCCT TCAAAATGGC TGGCAGGAGG
    ACGCGATTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT
    TGCAACCAGC TCTGTAGGAC CAGCAGCACT AACACAGTCG CACCATCAAC TGCCCCGGCC
    CCCCAGCCAA ACGTGCCCCC CGTGTCATCC AGCCGCAAGG GAACCTTCAC GGACGATCTA
    CACAAACTGG TGGACAACTG GGCCCGAGAC GCCATGAACC TGACTGGAAA AAAGGCTTGT
    AAGGCCTCTG GGCAACCCAG CTATGAGGGA CCCGGGATGG CCCGTAAGTT CTCTGCTCCC
    GGACAGCTGT GTGTCCCCAT GAACCCATCC CTGGGGGGCT CCGTCCCTCT CCCAGCAGCT
    TCGGCGACCT CCCTGGCTCA CTACCCCAAA GGAATGTGCC CCCCGCAACA ATACGGAATC
    CCCGGGGCGC TGTTCCAAGC TCCATGGAGT GGCACCGGCG TGCCCCCCTC CTCTCAGCCC
    CTAAGCCAGT TCCCCCCGGC GGTGACATCT TCCTTACAGA ACTTCGGTAT TAGCAGCTTG
    CAGAAATCTA TCAGCAACCC CCAAGGATCC AACCTGCGGA CTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54263
    Clone ID Related Accession (Same CDS sequence) XM_031898622.1
    Accession Version XM_031898622.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGGTGACC
    ACGGCCGGGG CAGAGACCCC AGCAGGCAGT CTCCCTGGTG AACACCTGCC TCCTCCTTCT
    CTCCCAGGAC CTTCACTAAC TCAGTCCCAG CAGCCTCTAG AAGACCTGGA TTTCCAGCTG
    AGAAGGACGT TGAGCCCTGA CACTGTCCCT GTGCCTTCTG CACTAATATT TCCTGCACAC
    AGCGGAGCTT CTACCGGTGT TGCAGCACCC GTCGGCTCAG CATTGCAACC CTCCGCTTCA
    GAATTGCCAA AAGCCAGCGC TGGAGAGGCA GAGGTGGGCG CGAGTGTCCA GAGGATGGGC
    AGGTTTCAGG TCTCTTTGGC AGCAGAGAAT GTGCTCAAGC AGAGCGAGGA GACCAAACTG
    TCCATCTCAG ACACTTCCTC CACCACTTCT ACATCCTCCT CTTCCTCTGC GTCATGCAGC
    AGCCCAGAGA GCACCCTGAC TAAGCCCCAG GCCAAAACCG CTGCTCCCTT AGACATAGTG
    GATGGTGTTG GTCCCCACTT GGTGCATCTA CAGAGCCTAT CTCCCAAGCA GTTGGTGAAA
    GTGGGGCGCT TCCAGGTTAC CACTGCCACC ACGGACAAAG TGGGGCGCTT TTCTGTGTCC
    CGGACACAAG ATGATATATC TGCTGGGGTG CTGGAGACAG ACTCCCAGAG GTCCTCCTCC
    CCACAGACTG AGAAACATCT GAATGGACCC TACTTACAGC AAGACTCCTC TGCGGCTCTC
    GGCTATCCGG CCAGGGAGAC GCAGGAAGAC AGCCTAGGGA GCCCCGTTCT GCACCACGCT
    TTGGGGTCCA AGCTTAGCCT GCAGAGCCTT AGCAACTCGT TCAACTCTTC CTACATGAGC
    AGCGATAATG ATTCTGACAT AGAAGATGAA GATCTGAAGA AGGAGCTGCG AAGGCTACGA
    GACAAGCATC TCAAGGAGAT TCAGGAACTG CAGAGTCGAC AGAAGCAGGA GATCGAGCTG
    TTGTACACGG CTTTGGGAAA AGTGCCCCCT GCTGTCATTA TCCCTCCGGC AGCGCCCCTT
    TCTGGCCGGC GCAGGAGGCA TACCAAGGGG AAGGGCAGCA AGTCCAGCCG GGGCAGCTCC
    CACAACAGCC GCAGCCCTCA TTTGTCAGGC GACCTGTCCA CGCAGAGCGC TCCGTCGGCA
    TTACCCCCCC AGCCGGGCCT TCTGCTCCCC CCAAGCAGTG CGCCCGAACA TGCTCATCTT
    CTCAAGCCCT CCCCTTCCAG CGACATTCTC TGCTCAGCCT TCACTAGCGA CGCTGCCCTC
    TCTGTGCCCA GCCTGTCCAC CCAGGGCCAA GGGACCAGCA GCACTAACAC AGTCGCACCA
    TCAACTGCCC CGGCCCCCCA GCCAAACGTG CCCCCCGTGT CATCCAGCCG CAAGGGAACC
    TTCACGGACG ATCTACACAA ACTGGTGGAC AACTGGGCCC GAGACGCCAT GAACCTGACT
    GGAAAAAAGG CTTGTAAGGC CTCTGGGCAA CCCAGCTATG AGGGACCCGG GATGGCCCGT
    AAGTTCTCTG CTCCCGGACA GCTGTGTGTC CCCATGAACC CATCCCTGGG GGGCTCCGTC
    CCTCTCCCAG CAGCTTCGGC GACCTCCCTG GCTCACTACC CCAAAGGAAT GTGCCCCCCG
    CAACAATACG GAATCCCCGG GGCGCTGTTC CAAGCTCCAT GGAGTGGCAC CGGCGTGCCC
    CCCTCCTCTC AGCCCCTAAG CCAGTTCCCC CCGGCGGTGA CATCTTCCTT ACAGAACTTC
    GGTATTAGCA GCTTGCAGAA ATCTATCAGC AACCCCCAAG GATCCAACCT GCGGACTACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754482.1
    CDS327..8549
    Translation

    Target ORF information:

    RefSeq Version XM_031898622.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898622.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGGTGACC
    ACGGCCGGGG CAGAGACCCC AGCAGGCAGT CTCCCTGGTG AACACCTGCC TCCTCCTTCT
    CTCCCAGGAC CTTCACTAAC TCAGTCCCAG CAGCCTCTAG AAGACCTGGA TTTCCAGCTG
    AGAAGGACGT TGAGCCCTGA CACTGTCCCT GTGCCTTCTG CACTAATATT TCCTGCACAC
    AGCGGAGCTT CTACCGGTGT TGCAGCACCC GTCGGCTCAG CATTGCAACC CTCCGCTTCA
    GAATTGCCAA AAGCCAGCGC TGGAGAGGCA GAGGTGGGCG CGAGTGTCCA GAGGATGGGC
    AGGTTTCAGG TCTCTTTGGC AGCAGAGAAT GTGCTCAAGC AGAGCGAGGA GACCAAACTG
    TCCATCTCAG ACACTTCCTC CACCACTTCT ACATCCTCCT CTTCCTCTGC GTCATGCAGC
    AGCCCAGAGA GCACCCTGAC TAAGCCCCAG GCCAAAACCG CTGCTCCCTT AGACATAGTG
    GATGGTGTTG GTCCCCACTT GGTGCATCTA CAGAGCCTAT CTCCCAAGCA GTTGGTGAAA
    GTGGGGCGCT TCCAGGTTAC CACTGCCACC ACGGACAAAG TGGGGCGCTT TTCTGTGTCC
    CGGACACAAG ATGATATATC TGCTGGGGTG CTGGAGACAG ACTCCCAGAG GTCCTCCTCC
    CCACAGACTG AGAAACATCT GAATGGACCC TACTTACAGC AAGACTCCTC TGCGGCTCTC
    GGCTATCCGG CCAGGGAGAC GCAGGAAGAC AGCCTAGGGA GCCCCGTTCT GCACCACGCT
    TTGGGGTCCA AGCTTAGCCT GCAGAGCCTT AGCAACTCGT TCAACTCTTC CTACATGAGC
    AGCGATAATG ATTCTGACAT AGAAGATGAA GATCTGAAGA AGGAGCTGCG AAGGCTACGA
    GACAAGCATC TCAAGGAGAT TCAGGAACTG CAGAGTCGAC AGAAGCAGGA GATCGAGCTG
    TTGTACACGG CTTTGGGAAA AGTGCCCCCT GCTGTCATTA TCCCTCCGGC AGCGCCCCTT
    TCTGGCCGGC GCAGGAGGCA TACCAAGGGG AAGGGCAGCA AGTCCAGCCG GGGCAGCTCC
    CACAACAGCC GCAGCCCTCA TTTGTCAGGC GACCTGTCCA CGCAGAGCGC TCCGTCGGCA
    TTACCCCCCC AGCCGGGCCT TCTGCTCCCC CCAAGCAGTG CGCCCGAACA TGCTCATCTT
    CTCAAGCCCT CCCCTTCCAG CGACATTCTC TGCTCAGCCT TCACTAGCGA CGCTGCCCTC
    TCTGTGCCCA GCCTGTCCAC CCAGGGCCAA GGGACCAGCA GCACTAACAC AGTCGCACCA
    TCAACTGCCC CGGCCCCCCA GCCAAACGTG CCCCCCGTGT CATCCAGCCG CAAGGGAACC
    TTCACGGACG ATCTACACAA ACTGGTGGAC AACTGGGCCC GAGACGCCAT GAACCTGACT
    GGAAAAAAGG CTTGTAAGGC CTCTGGGCAA CCCAGCTATG AGGGACCCGG GATGGCCCGT
    AAGTTCTCTG CTCCCGGACA GCTGTGTGTC CCCATGAACC CATCCCTGGG GGGCTCCGTC
    CCTCTCCCAG CAGCTTCGGC GACCTCCCTG GCTCACTACC CCAAAGGAAT GTGCCCCCCG
    CAACAATACG GAATCCCCGG GGCGCTGTTC CAAGCTCCAT GGAGTGGCAC CGGCGTGCCC
    CCCTCCTCTC AGCCCCTAAG CCAGTTCCCC CCGGCGGTGA CATCTTCCTT ACAGAACTTC
    GGTATTAGCA GCTTGCAGAA ATCTATCAGC AACCCCCAAG GATCCAACCT GCGGACTACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54264
    Clone ID Related Accession (Same CDS sequence) XM_031898623.1
    Accession Version XM_031898623.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGCCTGCA
    CACAGCGGAG CTTCTACCGG TGTTGCAGCA CCCGTCGGCT CAGCATTGCA ACCCTCCGCT
    TCAGAATTGC CAAAAGCCAG CGCTGGAGAG GCAGAGGTGG GCGCGAGTGT CCAGAGGATG
    GGCAGGTTTC AGGTCTCTTT GGCAGCAGAG AATGTGCTCA AGCAGAGCGA GGAGACCAAA
    CTGTCCATCT CAGACACTTC CTCCACCACT TCTACATCCT CCTCTTCCTC TGCGTCATGC
    AGCAGCCCAG AGAGCACCCT GACTAAGCCC CAGGCCAAAA CCGCTGCTCC CTTAGACATA
    GTGGATGGTG TTGGTCCCCA CTTGGTGCAT CTACAGAGCC TATCTCCCAA GCAGTTGGTG
    AAAGTGGGGC GCTTCCAGGT TACCACTGCC ACCACGGACA AAGTGGGGCG CTTTTCTGTG
    TCCCGGACAC AAGATGATAT ATCTGCTGGG GTGCTGGAGA CAGACTCCCA GAGGTCCTCC
    TCCCCACAGA CTGAGAAACA TCTGAATGGA CCCTACTTAC AGCAAGACTC CTCTGCGGCT
    CTCGGCTATC CGGCCAGGGA GACGCAGGAA GACAGCCTAG GGAGCCCCGT TCTGCACCAC
    GCTTTGGGGT CCAAGCTTAG CCTGCAGAGC CTTAGCAACT CGTTCAACTC TTCCTACATG
    AGCAGCGATA ATGATTCTGA CATAGAAGAT GAAGATCTGA AGAAGGAGCT GCGAAGGCTA
    CGAGACAAGC ATCTCAAGGA GATTCAGGAA CTGCAGAGTC GACAGAAGCA GGAGATCGAG
    CTGTTGTACA CGGCTTTGGG AAAAGTGCCC CCTGCTGTCA TTATCCCTCC GGCAGCGCCC
    CTTTCTGGCC GGCGCAGGAG GCATACCAAG GGGAAGGGCA GCAAGTCCAG CCGGGGCAGC
    TCCCACAACA GCCGCAGCCC TCATTTGTCA GGCGACCTGT CCACGCAGAG CGCTCCGTCG
    GCATTACCCC CCCAGCCGGG CCTTCTGCTC CCCCCAAGCA GTGCGCCCGA ACATGCTCAT
    CTTCTCAAGC CCTCCCCTTC CAGCGACATT CTCTGCTCAG CCTTCACTAG CGACGCTGCC
    CTCTCTGTGC CCAGCCTGTC CACCCAGGGC CAAGGCTGGG CCAAGTTTAA CTGTGAGTCA
    GAGCGGGTAT CCTTCAAAAT GGCTGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAG GACCAGCAGC
    ACTAACACAG TCGCACCATC AACTGCCCCG GCCCCCCAGC CAAACGTGCC CCCCGTGTCA
    TCCAGCCGCA AGGGAACCTT CACGGACGAT CTACACAAAC TGGTGGACAA CTGGGCCCGA
    GACGCCATGA ACCTGACTGG AAAAAAGGCT TGTAAGGCCT CTGGGCAACC CAGCTATGAG
    GGACCCGGGA TGGCCCGTAA GTTCTCTGCT CCCGGACAGC TGTGTGTCCC CATGAACCCA
    TCCCTGGGGG GCTCCGTCCC TCTCCCAGCA GCTTCGGCGA CCTCCCTGGC TCACTACCCC
    AAAGGAATGT GCCCCCCGCA ACAATACGGA ATCCCCGGGG CGCTGTTCCA AGCTCCATGG
    AGTGGCACCG GCGTGCCCCC CTCCTCTCAG CCCCTAAGCC AGTTCCCCCC GGCGGTGACA
    TCTTCCTTAC AGAACTTCGG TATTAGCAGC TTGCAGAAAT CTATCAGCAA CCCCCAAGGA
    TCCAACCTGC GGACTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754483.1
    CDS328..8508
    Translation

    Target ORF information:

    RefSeq Version XM_031898623.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898623.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCT TGTCGAGGTC GTAGCATGTC AGTATGTGTT
    CCCAACCTTT CCTCTGCTCC CCCACTCCTT TCCGTTGTGC CCCCCAGTGC TCCAGGCTCC
    CCTTCCCCAT TGCAGTCTGC CCCTGCCTCC CCACTCTGGA CATCTCCCGA AGATTCCTTT
    GCTGAAAAGC TTTCCAAGGC TCTAGAGAGT GTTCTGCCCA TGCACTCCTC CAGCCCTCGC
    AAGCACCAAC GCCGCTGCAG CCTGCCTACC CTCTATACCA GCCCTCCATC GTCCATGGTC
    CATTCAGCAC TCCCAGACTC TCCGCTTTTT TTCCCAACCA TTCCCAACCA GCGCCCCATG
    TCTTTCTCTC CGCCGCCTAC TGGCTTGTCC CCAAAGGTGC CTGCAACCCA GCGACGCAAG
    AGCACCTCTT TCTTGGAGTC CCAAGGCCGC CAGGGGCTAT CTCAGATTAG GACACTCGGG
    CCAGGTGAGG AGACCCAGTG CTGGAGGGCA GACTACATGG CAGCTCTAGG TAAAGCATCT
    GTCCGATCCG AGGATGCTCA CAAGCATCAA AACTATGAGG GATTGGCGTT CCATGACGCA
    AGACATGTTC CGACAGTTCC AAATGAAACT CTCTTAAAGC CTGGGGAAGC ATTGTATCTA
    GCCAGTCTGC CCTCACAGGA GAGCTACAGC AGCATGGCTG CCCCTGAGCA TATGGAGGGC
    CACAAGCTGA CCCAGCTTGG CTCGTTTTAC GATTATCACA CTGGGCAAAC CTACATTGCG
    CGGCCCCTGA AGAGCCTAAG ACTGGAATCT GGGTTAAACC AGCCGTCGGC TTTGGGTAGC
    ATCTCCAACC CAATCTCCTC CGACCCACAC GCCATGTTTC ATCAGATGTT TGTGCCCCAG
    TCTGCTCCTC CAGTGCTGGC TCAAAGCAGC GAAGGCCACC CAGGGTGTGT GTTTGAGTTC
    CACGTGCACC CATCTGGGTC TGTTCCCGGC GAAGCAACCG TTTACTTGCC ACACCGAGTT
    TACCGTCCTC GACGGGGCTC TGTAGACTTT AACCCAGAAG AACAGCCGCC GGGTTCTACG
    CTGCAGAGCC GCCTCCAAAC TGTGACTGAA GAGCAGTGCC ACCACAATGG CCCAGAACCA
    CTCCCACCCT TTATAGGATT CATCACATGC AACCGTATGG AGAGCAGCCC CGACTATTCC
    AGTGATTCTT CCCAAAGGAA TTCATCCGAT CCCGATTATC TCTCTCCTCC TCTGGCCTTC
    AGCTCCAATA TCTCTATGGC TTCCAAGCAG AAAACGTTGC AGGATCCACA GATTTTCTTT
    TGTGTCCCAC AGTCCGGAAC GACGGCTTAT GCTGGACCAT CAGCTTTGTA CAGCCAGCAG
    CCACAAATAT CATCTGGACA ACCACCCACC ACATCCATGG GAATCAGTGC TCAGCCCGGT
    CCCCACACCC AGGGCATGGT GCATCATCAG CCTTCTCTGC ATCCAGCCTC GCAGTACCAA
    GTGCAGCAGC CAATGCCTAC TGTGGGTTCC AGTATTGCGG CCCCATCACA GCCGGTGATT
    ACCCAAACGC CAGCCCCTCA GCCGGGCACC CAGCTCCCTG CCTCCCAGCC TTTGCCTGCT
    GTTCAAGGAG AGCCTCAGAT TCCTGCTCAG ACAGCTCCTG CTCCCCAGCC CGTTCCTACA
    GTGCCGGCCT CTCTGCCACC TCATTTTCTT CCCTTGGCAC AATCTTTGGT TGCAGTCCCT
    CTCATTCCTC CATTCGCTGC CCCACAGTTG CCTTTAACGG TACCACCCCC GGTCATACAG
    ACAGGGTTCC CTTCCCTACC CATGACTGTA ACCCCAAGTA TTGGCCAAAC TGTACCAGGA
    ATACCCCCAT CCTTAGGAGC GCCAGTCCTG CCCCATGGGC AGGCAGTGTT AGTTCCCACT
    CACATCACTA ATGTTGCTCT CCAGCCTTTG GCACCAATCC CCACACCTCC TCTGTGCCCT
    GTAACTCAGC CCATTGGATT ACCGGCCACC CTGGGAAAAG CTGCTGAGCC CTTGCTTCCT
    CCGGGAGATA CTTCTTACCA GGTTTTCCAG GCTACTCACC CTCTATTCCC TGGAGATGTA
    AGCTTTGTTC CTCCCGTTGC CATCCCCTCA GCAATGGTAT CTCCCCCTAT CCGTGGTGAA
    AGTTTAGCCC AACCTGGCTT CCATACTCAG CCTTATCCTG AAAACAGCAA TTTGTATTCC
    CATGGAGGGC CCACTCCCCA GCCCGCTGTT CTCACTACCC AGCCGGTGCC TGCAGGAACC
    CAGACGCAAC CTGCCGTCGA GGGTTCACAG AGTAGCTCCC TGGCAATTTC CCAGGATACC
    CAGTCATCTC CTCAAGCAAC ACAAACTGCC TCCTCCATGG ACAGCGCTCA TTCAGACGTG
    GCATCAGGAC TGAGTGACGG AAACGAGGGC AGCACAGGTG GGAGACACGA AGGCCGAGCC
    ACTAAACGAC ACTACCGAAA ATCTGTGAGA AGCCGCTCCA GGCACGAGAA AACTGCACGC
    CCAAAGCTTC GAATCCTTAA TGTTTCCAAC AAAGGGGATC GTGTTGTAGA GTGTCAGCTG
    GAGACTCACA ACCGCAAGAT GGTCACCTTC AAATTCGACC TGGATGGAGA CAATCCAGAG
    GAGATTGCTA CTATAATGGT ACAGAATGTG TTTATTTTGG CTACGGAGAG AGAATCCTTT
    GTAGAGCAGG TAAAAGACAT AATTGAGAAG GCCGATGAAA TGTTGAGCGA AGATGTGAGC
    GTGGAGCCGG AAGGAGAAAC GAGGGCAGAA AGTTTGCAAA TCAAGGATGA TGGGGATTAC
    CCAGAATCTC AGAAGATGAA TTCTGAATTT CGAATGCCAG ATCCTGTATA TTCCCTACCA
    CAGACAGCAG GGCTTATTCC CAGCGTTGTC ACACAAGTCG TTCATTCTGC CGGAAGACGA
    TTTATTGTCA GTCCGGTGCC TGAAAGCCGT CTAAAAGAAC AGTCATTCTT CAGTACTTCA
    TTGCAGGACC CTCAGCTACC AGTCTCTGTC GCGCATGGTC CAGGGATGAA TTTGTCTCAT
    TCTGCCTCTT CTGTGAGCCT GCAGCAAGCC TTTGCAGAAC TACGCCAGTC ACAGATGAGC
    GAGGGTCCTA GCACAGCTCC TCCCATATTT AACCAAACTG GACCCCCCTT TCCACCATCC
    ATGACTAGTC TGGTGGCAAC TCTGCCTTCT AATGCAATGC CTATCCTCCA GGCAGCCCCA
    GTGATCCCCA GTAGCCTGCC ATCTTCTGTG CCTCAGCCCC TGGTGGAGGT GGTTGTACCT
    CAGGCTGCTG GGATGGCTCC AACTAGCAAT GTTCCAAGCA CCTTGTCCCC TCCCCTAACT
    GCAGTATCCA ACCAGCACGC AGACATGATA CCGGGTGCTG CTGTAGCAGC TATCGGATCA
    CTTCCTGTGA CATCACTTCC TCCTTTGCCC ATTGTGGGTG GAGTTCCTGT AGGCACTGCA
    ACCCTGAGCT CACCCCTAAC ACCCCTAATC AGTTCTGTCA GTACAATGGG GGGGACGGTT
    CCCCCATCTG TCACATCTCC CCCAGTGCCA CAGATAGCGG GTAGCGTTGC CGGGAGCCTG
    CCTCCTGCAT CAGCCTCCGC CCCTCTTATT CATGGCAGTG TTGCCCCATC TCAGGTACCG
    CTTTGCAGCA GCAGTTCTGT TCCCAGCTTT GCAGAGAGCG TGGCAGTCAG CCCCCTGCTG
    GATAGTGGCC AAAAGGCAGC AGAAGTGGGC ACGGTAATGA GCACGACTGG CTTGTCTCTG
    CCCATCTCTG CACCTCTGAC ATATGCTGGG GTGGTTGCAG CATCAGGCAT CACAAGTCAA
    CCTGCCGTGC ATCTCCCCCA GGGGGTGGCC ACAACACCAG TTGTATCCCA GGCAGTAGGG
    GGAGCTGTGG GCACACCCCT CTCATCTCTT CCTCCCATCC CTGGAGGCAT GTCTGTTAGC
    CAACCAGGAT CAAATATACC CCCTGTGCAA CACACGTTGG CCCATGGCCA ACCCCAGCCT
    ACCCCTCTCC CCAACCAGCC TCATACGCAC CTTGTAGAGG GTGACACCGA TAACCTGCCC
    AAACCTGGCA TAGACGATAT AAAAACCCTG GAGGAGAAGC TGAGGTCGCT GTTCAGTGAG
    CATGGCAGTG CCGGGGCCCC TCATGGACTC ACCCCATCAG AAACTCAATT GGGTTTGGAT
    GGGGCCCCCC TGCCAGGCCC TTCCACAGGT AGCACCGCTC AGACCAAGCC TGCAGCATCC
    TCTTCTCCAC CAACCAGCTT ACCTTTAGGG CCACCTGGAG TGACGACAAT GACTCAGATC
    ATGACACCTG GACAAGCTGC TACACCTGTC ACCTACGTAT CTGCAGCTGG AAGCTCTATA
    GCCACGGCCA AAGCTGCCAC CCCACCCTCA AAGCCGCCTC TCAGTCGGGT ACCGCCTGCA
    CACAGCGGAG CTTCTACCGG TGTTGCAGCA CCCGTCGGCT CAGCATTGCA ACCCTCCGCT
    TCAGAATTGC CAAAAGCCAG CGCTGGAGAG GCAGAGGTGG GCGCGAGTGT CCAGAGGATG
    GGCAGGTTTC AGGTCTCTTT GGCAGCAGAG AATGTGCTCA AGCAGAGCGA GGAGACCAAA
    CTGTCCATCT CAGACACTTC CTCCACCACT TCTACATCCT CCTCTTCCTC TGCGTCATGC
    AGCAGCCCAG AGAGCACCCT GACTAAGCCC CAGGCCAAAA CCGCTGCTCC CTTAGACATA
    GTGGATGGTG TTGGTCCCCA CTTGGTGCAT CTACAGAGCC TATCTCCCAA GCAGTTGGTG
    AAAGTGGGGC GCTTCCAGGT TACCACTGCC ACCACGGACA AAGTGGGGCG CTTTTCTGTG
    TCCCGGACAC AAGATGATAT ATCTGCTGGG GTGCTGGAGA CAGACTCCCA GAGGTCCTCC
    TCCCCACAGA CTGAGAAACA TCTGAATGGA CCCTACTTAC AGCAAGACTC CTCTGCGGCT
    CTCGGCTATC CGGCCAGGGA GACGCAGGAA GACAGCCTAG GGAGCCCCGT TCTGCACCAC
    GCTTTGGGGT CCAAGCTTAG CCTGCAGAGC CTTAGCAACT CGTTCAACTC TTCCTACATG
    AGCAGCGATA ATGATTCTGA CATAGAAGAT GAAGATCTGA AGAAGGAGCT GCGAAGGCTA
    CGAGACAAGC ATCTCAAGGA GATTCAGGAA CTGCAGAGTC GACAGAAGCA GGAGATCGAG
    CTGTTGTACA CGGCTTTGGG AAAAGTGCCC CCTGCTGTCA TTATCCCTCC GGCAGCGCCC
    CTTTCTGGCC GGCGCAGGAG GCATACCAAG GGGAAGGGCA GCAAGTCCAG CCGGGGCAGC
    TCCCACAACA GCCGCAGCCC TCATTTGTCA GGCGACCTGT CCACGCAGAG CGCTCCGTCG
    GCATTACCCC CCCAGCCGGG CCTTCTGCTC CCCCCAAGCA GTGCGCCCGA ACATGCTCAT
    CTTCTCAAGC CCTCCCCTTC CAGCGACATT CTCTGCTCAG CCTTCACTAG CGACGCTGCC
    CTCTCTGTGC CCAGCCTGTC CACCCAGGGC CAAGGCTGGG CCAAGTTTAA CTGTGAGTCA
    GAGCGGGTAT CCTTCAAAAT GGCTGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAG GACCAGCAGC
    ACTAACACAG TCGCACCATC AACTGCCCCG GCCCCCCAGC CAAACGTGCC CCCCGTGTCA
    TCCAGCCGCA AGGGAACCTT CACGGACGAT CTACACAAAC TGGTGGACAA CTGGGCCCGA
    GACGCCATGA ACCTGACTGG AAAAAAGGCT TGTAAGGCCT CTGGGCAACC CAGCTATGAG
    GGACCCGGGA TGGCCCGTAA GTTCTCTGCT CCCGGACAGC TGTGTGTCCC CATGAACCCA
    TCCCTGGGGG GCTCCGTCCC TCTCCCAGCA GCTTCGGCGA CCTCCCTGGC TCACTACCCC
    AAAGGAATGT GCCCCCCGCA ACAATACGGA ATCCCCGGGG CGCTGTTCCA AGCTCCATGG
    AGTGGCACCG GCGTGCCCCC CTCCTCTCAG CCCCTAAGCC AGTTCCCCCC GGCGGTGACA
    TCTTCCTTAC AGAACTTCGG TATTAGCAGC TTGCAGAAAT CTATCAGCAA CCCCCAAGGA
    TCCAACCTGC GGACTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54265
    Clone ID Related Accession (Same CDS sequence) XM_031898624.1
    Accession Version XM_031898624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCA TCGTCCATGG TCCATTCAGC ACTCCCAGAC
    TCTCCGCTTT TTTTCCCAAC CATTCCCAAC CAGCGCCCCA TGTCTTTCTC TCCGCCGCCT
    ACTGGCTTGT CCCCAAAGGT GCCTGCAACC CAGCGACGCA AGAGCACCTC TTTCTTGGAG
    TCCCAAGGCC GCCAGGGGCT ATCTCAGATT AGGACACTCG GGCCAGGTGA GGAGACCCAG
    TGCTGGAGGG CAGACTACAT GGCAGCTCTA GGTAAAGCAT CTGTCCGATC CGAGGATGCT
    CACAAGCATC AAAACTATGA GGGATTGGCG TTCCATGACG CAAGACATGT TCCGACAGTT
    CCAAATGAAA CTCTCTTAAA GCCTGGGGAA GCATTGTATC TAGCCAGTCT GCCCTCACAG
    GAGAGCTACA GCAGCATGGC TGCCCCTGAG CATATGGAGG GCCACAAGCT GACCCAGCTT
    GGCTCGTTTT ACGATTATCA CACTGGGCAA ACCTACATTG CGCGGCCCCT GAAGAGCCTA
    AGACTGGAAT CTGGGTTAAA CCAGCCGTCG GCTTTGGGTA GCATCTCCAA CCCAATCTCC
    TCCGACCCAC ACGCCATGTT TCATCAGATG TTTGTGCCCC AGTCTGCTCC TCCAGTGCTG
    GCTCAAAGCA GCGAAGGCCA CCCAGGGTGT GTGTTTGAGT TCCACGTGCA CCCATCTGGG
    TCTGTTCCCG GCGAAGCAAC CGTTTACTTG CCACACCGAG TTTACCGTCC TCGACGGGGC
    TCTGTAGACT TTAACCCAGA AGAACAGCCG CCGGGTTCTA CGCTGCAGAG CCGCCTCCAA
    ACTGTGACTG AAGAGCAGTG CCACCACAAT GGCCCAGAAC CACTCCCACC CTTTATAGGA
    TTCATCACAT GCAACCGTAT GGAGAGCAGC CCCGACTATT CCAGTGATTC TTCCCAAAGG
    AATTCATCCG ATCCCGATTA TCTCTCTCCT CCTCTGGCCT TCAGCTCCAA TATCTCTATG
    GCTTCCAAGC AGAAAACGTT GCAGGATCCA CAGATTTTCT TTTGTGTCCC ACAGTCCGGA
    ACGACGGCTT ATGCTGGACC ATCAGCTTTG TACAGCCAGC AGCCACAAAT ATCATCTGGA
    CAACCACCCA CCACATCCAT GGGAATCAGT GCTCAGCCCG GTCCCCACAC CCAGGGCATG
    GTGCATCATC AGCCTTCTCT GCATCCAGCC TCGCAGTACC AAGTGCAGCA GCCAATGCCT
    ACTGTGGGTT CCAGTATTGC GGCCCCATCA CAGCCGGTGA TTACCCAAAC GCCAGCCCCT
    CAGCCGGGCA CCCAGCTCCC TGCCTCCCAG CCTTTGCCTG CTGTTCAAGG AGAGCCTCAG
    ATTCCTGCTC AGACAGCTCC TGCTCCCCAG CCCGTTCCTA CAGTGCCGGC CTCTCTGCCA
    CCTCATTTTC TTCCCTTGGC ACAATCTTTG GTTGCAGTCC CTCTCATTCC TCCATTCGCT
    GCCCCACAGT TGCCTTTAAC GGTACCACCC CCGGTCATAC AGACAGGGTT CCCTTCCCTA
    CCCATGACTG TAACCCCAAG TATTGGCCAA ACTGTACCAG GAATACCCCC ATCCTTAGGA
    GCGCCAGTCC TGCCCCATGG GCAGGCAGTG TTAGTTCCCA CTCACATCAC TAATGTTGCT
    CTCCAGCCTT TGGCACCAAT CCCCACACCT CCTCTGTGCC CTGTAACTCA GCCCATTGGA
    TTACCGGCCA CCCTGGGAAA AGCTGCTGAG CCCTTGCTTC CTCCGGGAGA TACTTCTTAC
    CAGGTTTTCC AGGCTACTCA CCCTCTATTC CCTGGAGATG TAAGCTTTGT TCCTCCCGTT
    GCCATCCCCT CAGCAATGGT ATCTCCCCCT ATCCGTGGTG AAAGTTTAGC CCAACCTGGC
    TTCCATACTC AGCCTTATCC TGAAAACAGC AATTTGTATT CCCATGGAGG GCCCACTCCC
    CAGCCCGCTG TTCTCACTAC CCAGCCGGTG CCTGCAGGAA CCCAGACGCA ACCTGCCGTC
    GAGGGTTCAC AGAGTAGCTC CCTGGCAATT TCCCAGGATA CCCAGTCATC TCCTCAAGCA
    ACACAAACTG CCTCCTCCAT GGACAGCGCT CATTCAGACG TGGCATCAGG ACTGAGTGAC
    GGAAACGAGG GCAGCACAGG TGGGAGACAC GAAGGCCGAG CCACTAAACG ACACTACCGA
    AAATCTGTGA GAAGCCGCTC CAGGCACGAG AAAACTGCAC GCCCAAAGCT TCGAATCCTT
    AATGTTTCCA ACAAAGGGGA TCGTGTTGTA GAGTGTCAGC TGGAGACTCA CAACCGCAAG
    ATGGTCACCT TCAAATTCGA CCTGGATGGA GACAATCCAG AGGAGATTGC TACTATAATG
    GTACAGAATG TGTTTATTTT GGCTACGGAG AGAGAATCCT TTGTAGAGCA GGTAAAAGAC
    ATAATTGAGA AGGCCGATGA AATGTTGAGC GAAGATGTGA GCGTGGAGCC GGAAGGAGAA
    ACGAGGGCAG AAAGTTTGCA AATCAAGGAT GATGGGGATT ACCCAGAATC TCAGAAGATG
    AATTCTGAAT TTCGAATGCC AGATCCTGTA TATTCCCTAC CACAGACAGC AGGGCTTATT
    CCCAGCGTTG TCACACAAGT CGTTCATTCT GCCGGAAGAC GATTTATTGT CAGTCCGGTG
    CCTGAAAGCC GTCTAAAAGA ACAGTCATTC TTCAGTACTT CATTGCAGGA CCCTCAGCTA
    CCAGTCTCTG TCGCGCATGG TCCAGGGATG AATTTGTCTC ATTCTGCCTC TTCTGTGAGC
    CTGCAGCAAG CCTTTGCAGA ACTACGCCAG TCACAGATGA GCGAGGGTCC TAGCACAGCT
    CCTCCCATAT TTAACCAAAC TGGACCCCCC TTTCCACCAT CCATGACTAG TCTGGTGGCA
    ACTCTGCCTT CTAATGCAAT GCCTATCCTC CAGGCAGCCC CAGTGATCCC CAGTAGCCTG
    CCATCTTCTG TGCCTCAGCC CCTGGTGGAG GTGGTTGTAC CTCAGGCTGC TGGGATGGCT
    CCAACTAGCA ATGTTCCAAG CACCTTGTCC CCTCCCCTAA CTGCAGTATC CAACCAGCAC
    GCAGACATGA TACCGGGTGC TGCTGTAGCA GCTATCGGAT CACTTCCTGT GACATCACTT
    CCTCCTTTGC CCATTGTGGG TGGAGTTCCT GTAGGCACTG CAACCCTGAG CTCACCCCTA
    ACACCCCTAA TCAGTTCTGT CAGTACAATG GGGGGGACGG TTCCCCCATC TGTCACATCT
    CCCCCAGTGC CACAGATAGC GGGTAGCGTT GCCGGGAGCC TGCCTCCTGC ATCAGCCTCC
    GCCCCTCTTA TTCATGGCAG TGTTGCCCCA TCTCAGGTAC CGCTTTGCAG CAGCAGTTCT
    GTTCCCAGCT TTGCAGAGAG CGTGGCAGTC AGCCCCCTGC TGGATAGTGG CCAAAAGGCA
    GCAGAAGTGG GCACGGTAAT GAGCACGACT GGCTTGTCTC TGCCCATCTC TGCACCTCTG
    ACATATGCTG GGGTGGTTGC AGCATCAGGC ATCACAAGTC AACCTGCCGT GCATCTCCCC
    CAGGGGGTGG CCACAACACC AGTTGTATCC CAGGCAGTAG GGGGAGCTGT GGGCACACCC
    CTCTCATCTC TTCCTCCCAT CCCTGGAGGC ATGTCTGTTA GCCAACCAGG ATCAAATATA
    CCCCCTGTGC AACACACGTT GGCCCATGGC CAACCCCAGC CTACCCCTCT CCCCAACCAG
    CCTCATACGC ACCTTGTAGA GGGTGACACC GATAACCTGC CCAAACCTGG CATAGACGAT
    ATAAAAACCC TGGAGGAGAA GCTGAGGTCG CTGTTCAGTG AGCATGGCAG TGCCGGGGCC
    CCTCATGGAC TCACCCCATC AGAAACTCAA TTGGGTTTGG ATGGGGCCCC CCTGCCAGGC
    CCTTCCACAG GTAGCACCGC TCAGACCAAG CCTGCAGCAT CCTCTTCTCC ACCAACCAGC
    TTACCTTTAG GGCCACCTGG AGTGACGACA ATGACTCAGA TCATGACACC TGGACAAGCT
    GCTACACCTG TCACCTACGT ATCTGCAGCT GGAAGCTCTA TAGCCACGGC CAAAGCTGCC
    ACCCCACCCT CAAAGCCGCC TCTCAGTCGG GTACCGGTGA CCACGGCCGG GGCAGAGACC
    CCAGCAGGCA GTCTCCCTGG TGAACACCTG CCTCCTCCTT CTCTCCCAGG ACCTTCACTA
    ACTCAGTCCC AGCAGCCTCT AGAAGACCTG GATTTCCAGC TGAGAAGGAC GTTGAGCCCT
    GACACTGTCC CTGTGCCTTC TGCACTAATA TTTCCTGCAC ACAGCGGAGC TTCTACCGGT
    GTTGCAGCAC CCGTCGGCTC AGCATTGCAA CCCTCCGCTT CAGAATTGCC AAAAGCCAGC
    GCTGGAGAGG CAGAGGTGGG CGCGAGTGTC CAGAGGATGG GCAGGTTTCA GGTCTCTTTG
    GCAGCAGAGA ATGTGCTCAA GCAGAGCGAG GAGACCAAAC TGTCCATCTC AGACACTTCC
    TCCACCACTT CTACATCCTC CTCTTCCTCT GCGTCATGCA GCAGCCCAGA GAGCACCCTG
    ACTAAGCCCC AGGCCAAAAC CGCTGCTCCC TTAGACATAG TGGATGGTGT TGGTCCCCAC
    TTGGTGCATC TACAGAGCCT ATCTCCCAAG CAGTTGGTGA AAGTGGGGCG CTTCCAGGTT
    ACCACTGCCA CCACGGACAA AGTGGGGCGC TTTTCTGTGT CCCGGACACA AGATGATATA
    TCTGCTGGGG TGCTGGAGAC AGACTCCCAG AGGTCCTCCT CCCCACAGAC TGAGAAACAT
    CTGAATGGAC CCTACTTACA GCAAGACTCC TCTGCGGCTC TCGGCTATCC GGCCAGGGAG
    ACGCAGGAAG ACAGCCTAGG GAGCCCCGTT CTGCACCACG CTTTGGGGTC CAAGCTTAGC
    CTGCAGAGCC TTAGCAACTC GTTCAACTCT TCCTACATGA GCAGCGATAA TGATTCTGAC
    ATAGAAGATG AAGATCTGAA GAAGGAGCTG CGAAGGCTAC GAGACAAGCA TCTCAAGGAG
    ATTCAGGAAC TGCAGAGTCG ACAGAAGCAG GAGATCGAGC TGTTGTACAC GGCTTTGGGA
    AAAGTGCCCC CTGCTGTCAT TATCCCTCCG GCAGCGCCCC TTTCTGGCCG GCGCAGGAGG
    CATACCAAGG GGAAGGGCAG CAAGTCCAGC CGGGGCAGCT CCCACAACAG CCGCAGCCCT
    CATTTGTCAG GCGACCTGTC CACGCAGAGC GCTCCGTCGG CATTACCCCC CCAGCCGGGC
    CTTCTGCTCC CCCCAAGCAG TGCGCCCGAA CATGCTCATC TTCTCAAGCC CTCCCCTTCC
    AGCGACATTC TCTGCTCAGC CTTCACTAGC GACGCTGCCC TCTCTGTGCC CAGCCTGTCC
    ACCCAGGGCC AAGGCTGGGC CAAGTTTAAC TGTGAGTCAG AGCGGGTATC CTTCAAAATG
    GCTGGCAGGA GGACGCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA
    AAAGTCTGTC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CTAACACAGT CGCACCATCA
    ACTGCCCCGG CCCCCCAGCC AAACGTGCCC CCCGTGTCAT CCAGCCGCAA GGGAACCTTC
    ACGGACGATC TACACAAACT GGTGGACAAC TGGGCCCGAG ACGCCATGAA CCTGACTGGA
    AAAAAGGCTT GTAAGGCCTC TGGGCAACCC AGCTATGAGG GACCCGGGAT GGCCCGTAAG
    TTCTCTGCTC CCGGACAGCT GTGTGTCCCC ATGAACCCAT CCCTGGGGGG CTCCGTCCCT
    CTCCCAGCAG CTTCGGCGAC CTCCCTGGCT CACTACCCCA AAGGAATGTG CCCCCCGCAA
    CAATACGGAA TCCCCGGGGC GCTGTTCCAA GCTCCATGGA GTGGCACCGG CGTGCCCCCC
    TCCTCTCAGC CCCTAAGCCA GTTCCCCCCG GCGGTGACAT CTTCCTTACA GAACTTCGGT
    ATTAGCAGCT TGCAGAAATC TATCAGCAAC CCCCAAGGAT CCAACCTGCG GACTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754484.1
    CDS327..8426
    Translation

    Target ORF information:

    RefSeq Version XM_031898624.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCATT AGCCGATGGG ACAATTGACA GTGGTCAGGG ATCGTCCATG
    TATTCTGAAT CTTATGCGGG AAACCAGCCG ACAGTCTCCT TTGGCTCTCA GCCCGATCCA
    AGCCTCACTC CCAGCATGGT GCCTGGCTTC AGCACTCCAA ATATCCAACC CCCTGCCCAG
    CATGGCGGAT ACCCAGCTCC TGTAGGGCCA TCGTCCATGG TCCATTCAGC ACTCCCAGAC
    TCTCCGCTTT TTTTCCCAAC CATTCCCAAC CAGCGCCCCA TGTCTTTCTC TCCGCCGCCT
    ACTGGCTTGT CCCCAAAGGT GCCTGCAACC CAGCGACGCA AGAGCACCTC TTTCTTGGAG
    TCCCAAGGCC GCCAGGGGCT ATCTCAGATT AGGACACTCG GGCCAGGTGA GGAGACCCAG
    TGCTGGAGGG CAGACTACAT GGCAGCTCTA GGTAAAGCAT CTGTCCGATC CGAGGATGCT
    CACAAGCATC AAAACTATGA GGGATTGGCG TTCCATGACG CAAGACATGT TCCGACAGTT
    CCAAATGAAA CTCTCTTAAA GCCTGGGGAA GCATTGTATC TAGCCAGTCT GCCCTCACAG
    GAGAGCTACA GCAGCATGGC TGCCCCTGAG CATATGGAGG GCCACAAGCT GACCCAGCTT
    GGCTCGTTTT ACGATTATCA CACTGGGCAA ACCTACATTG CGCGGCCCCT GAAGAGCCTA
    AGACTGGAAT CTGGGTTAAA CCAGCCGTCG GCTTTGGGTA GCATCTCCAA CCCAATCTCC
    TCCGACCCAC ACGCCATGTT TCATCAGATG TTTGTGCCCC AGTCTGCTCC TCCAGTGCTG
    GCTCAAAGCA GCGAAGGCCA CCCAGGGTGT GTGTTTGAGT TCCACGTGCA CCCATCTGGG
    TCTGTTCCCG GCGAAGCAAC CGTTTACTTG CCACACCGAG TTTACCGTCC TCGACGGGGC
    TCTGTAGACT TTAACCCAGA AGAACAGCCG CCGGGTTCTA CGCTGCAGAG CCGCCTCCAA
    ACTGTGACTG AAGAGCAGTG CCACCACAAT GGCCCAGAAC CACTCCCACC CTTTATAGGA
    TTCATCACAT GCAACCGTAT GGAGAGCAGC CCCGACTATT CCAGTGATTC TTCCCAAAGG
    AATTCATCCG ATCCCGATTA TCTCTCTCCT CCTCTGGCCT TCAGCTCCAA TATCTCTATG
    GCTTCCAAGC AGAAAACGTT GCAGGATCCA CAGATTTTCT TTTGTGTCCC ACAGTCCGGA
    ACGACGGCTT ATGCTGGACC ATCAGCTTTG TACAGCCAGC AGCCACAAAT ATCATCTGGA
    CAACCACCCA CCACATCCAT GGGAATCAGT GCTCAGCCCG GTCCCCACAC CCAGGGCATG
    GTGCATCATC AGCCTTCTCT GCATCCAGCC TCGCAGTACC AAGTGCAGCA GCCAATGCCT
    ACTGTGGGTT CCAGTATTGC GGCCCCATCA CAGCCGGTGA TTACCCAAAC GCCAGCCCCT
    CAGCCGGGCA CCCAGCTCCC TGCCTCCCAG CCTTTGCCTG CTGTTCAAGG AGAGCCTCAG
    ATTCCTGCTC AGACAGCTCC TGCTCCCCAG CCCGTTCCTA CAGTGCCGGC CTCTCTGCCA
    CCTCATTTTC TTCCCTTGGC ACAATCTTTG GTTGCAGTCC CTCTCATTCC TCCATTCGCT
    GCCCCACAGT TGCCTTTAAC GGTACCACCC CCGGTCATAC AGACAGGGTT CCCTTCCCTA
    CCCATGACTG TAACCCCAAG TATTGGCCAA ACTGTACCAG GAATACCCCC ATCCTTAGGA
    GCGCCAGTCC TGCCCCATGG GCAGGCAGTG TTAGTTCCCA CTCACATCAC TAATGTTGCT
    CTCCAGCCTT TGGCACCAAT CCCCACACCT CCTCTGTGCC CTGTAACTCA GCCCATTGGA
    TTACCGGCCA CCCTGGGAAA AGCTGCTGAG CCCTTGCTTC CTCCGGGAGA TACTTCTTAC
    CAGGTTTTCC AGGCTACTCA CCCTCTATTC CCTGGAGATG TAAGCTTTGT TCCTCCCGTT
    GCCATCCCCT CAGCAATGGT ATCTCCCCCT ATCCGTGGTG AAAGTTTAGC CCAACCTGGC
    TTCCATACTC AGCCTTATCC TGAAAACAGC AATTTGTATT CCCATGGAGG GCCCACTCCC
    CAGCCCGCTG TTCTCACTAC CCAGCCGGTG CCTGCAGGAA CCCAGACGCA ACCTGCCGTC
    GAGGGTTCAC AGAGTAGCTC CCTGGCAATT TCCCAGGATA CCCAGTCATC TCCTCAAGCA
    ACACAAACTG CCTCCTCCAT GGACAGCGCT CATTCAGACG TGGCATCAGG ACTGAGTGAC
    GGAAACGAGG GCAGCACAGG TGGGAGACAC GAAGGCCGAG CCACTAAACG ACACTACCGA
    AAATCTGTGA GAAGCCGCTC CAGGCACGAG AAAACTGCAC GCCCAAAGCT TCGAATCCTT
    AATGTTTCCA ACAAAGGGGA TCGTGTTGTA GAGTGTCAGC TGGAGACTCA CAACCGCAAG
    ATGGTCACCT TCAAATTCGA CCTGGATGGA GACAATCCAG AGGAGATTGC TACTATAATG
    GTACAGAATG TGTTTATTTT GGCTACGGAG AGAGAATCCT TTGTAGAGCA GGTAAAAGAC
    ATAATTGAGA AGGCCGATGA AATGTTGAGC GAAGATGTGA GCGTGGAGCC GGAAGGAGAA
    ACGAGGGCAG AAAGTTTGCA AATCAAGGAT GATGGGGATT ACCCAGAATC TCAGAAGATG
    AATTCTGAAT TTCGAATGCC AGATCCTGTA TATTCCCTAC CACAGACAGC AGGGCTTATT
    CCCAGCGTTG TCACACAAGT CGTTCATTCT GCCGGAAGAC GATTTATTGT CAGTCCGGTG
    CCTGAAAGCC GTCTAAAAGA ACAGTCATTC TTCAGTACTT CATTGCAGGA CCCTCAGCTA
    CCAGTCTCTG TCGCGCATGG TCCAGGGATG AATTTGTCTC ATTCTGCCTC TTCTGTGAGC
    CTGCAGCAAG CCTTTGCAGA ACTACGCCAG TCACAGATGA GCGAGGGTCC TAGCACAGCT
    CCTCCCATAT TTAACCAAAC TGGACCCCCC TTTCCACCAT CCATGACTAG TCTGGTGGCA
    ACTCTGCCTT CTAATGCAAT GCCTATCCTC CAGGCAGCCC CAGTGATCCC CAGTAGCCTG
    CCATCTTCTG TGCCTCAGCC CCTGGTGGAG GTGGTTGTAC CTCAGGCTGC TGGGATGGCT
    CCAACTAGCA ATGTTCCAAG CACCTTGTCC CCTCCCCTAA CTGCAGTATC CAACCAGCAC
    GCAGACATGA TACCGGGTGC TGCTGTAGCA GCTATCGGAT CACTTCCTGT GACATCACTT
    CCTCCTTTGC CCATTGTGGG TGGAGTTCCT GTAGGCACTG CAACCCTGAG CTCACCCCTA
    ACACCCCTAA TCAGTTCTGT CAGTACAATG GGGGGGACGG TTCCCCCATC TGTCACATCT
    CCCCCAGTGC CACAGATAGC GGGTAGCGTT GCCGGGAGCC TGCCTCCTGC ATCAGCCTCC
    GCCCCTCTTA TTCATGGCAG TGTTGCCCCA TCTCAGGTAC CGCTTTGCAG CAGCAGTTCT
    GTTCCCAGCT TTGCAGAGAG CGTGGCAGTC AGCCCCCTGC TGGATAGTGG CCAAAAGGCA
    GCAGAAGTGG GCACGGTAAT GAGCACGACT GGCTTGTCTC TGCCCATCTC TGCACCTCTG
    ACATATGCTG GGGTGGTTGC AGCATCAGGC ATCACAAGTC AACCTGCCGT GCATCTCCCC
    CAGGGGGTGG CCACAACACC AGTTGTATCC CAGGCAGTAG GGGGAGCTGT GGGCACACCC
    CTCTCATCTC TTCCTCCCAT CCCTGGAGGC ATGTCTGTTA GCCAACCAGG ATCAAATATA
    CCCCCTGTGC AACACACGTT GGCCCATGGC CAACCCCAGC CTACCCCTCT CCCCAACCAG
    CCTCATACGC ACCTTGTAGA GGGTGACACC GATAACCTGC CCAAACCTGG CATAGACGAT
    ATAAAAACCC TGGAGGAGAA GCTGAGGTCG CTGTTCAGTG AGCATGGCAG TGCCGGGGCC
    CCTCATGGAC TCACCCCATC AGAAACTCAA TTGGGTTTGG ATGGGGCCCC CCTGCCAGGC
    CCTTCCACAG GTAGCACCGC TCAGACCAAG CCTGCAGCAT CCTCTTCTCC ACCAACCAGC
    TTACCTTTAG GGCCACCTGG AGTGACGACA ATGACTCAGA TCATGACACC TGGACAAGCT
    GCTACACCTG TCACCTACGT ATCTGCAGCT GGAAGCTCTA TAGCCACGGC CAAAGCTGCC
    ACCCCACCCT CAAAGCCGCC TCTCAGTCGG GTACCGGTGA CCACGGCCGG GGCAGAGACC
    CCAGCAGGCA GTCTCCCTGG TGAACACCTG CCTCCTCCTT CTCTCCCAGG ACCTTCACTA
    ACTCAGTCCC AGCAGCCTCT AGAAGACCTG GATTTCCAGC TGAGAAGGAC GTTGAGCCCT
    GACACTGTCC CTGTGCCTTC TGCACTAATA TTTCCTGCAC ACAGCGGAGC TTCTACCGGT
    GTTGCAGCAC CCGTCGGCTC AGCATTGCAA CCCTCCGCTT CAGAATTGCC AAAAGCCAGC
    GCTGGAGAGG CAGAGGTGGG CGCGAGTGTC CAGAGGATGG GCAGGTTTCA GGTCTCTTTG
    GCAGCAGAGA ATGTGCTCAA GCAGAGCGAG GAGACCAAAC TGTCCATCTC AGACACTTCC
    TCCACCACTT CTACATCCTC CTCTTCCTCT GCGTCATGCA GCAGCCCAGA GAGCACCCTG
    ACTAAGCCCC AGGCCAAAAC CGCTGCTCCC TTAGACATAG TGGATGGTGT TGGTCCCCAC
    TTGGTGCATC TACAGAGCCT ATCTCCCAAG CAGTTGGTGA AAGTGGGGCG CTTCCAGGTT
    ACCACTGCCA CCACGGACAA AGTGGGGCGC TTTTCTGTGT CCCGGACACA AGATGATATA
    TCTGCTGGGG TGCTGGAGAC AGACTCCCAG AGGTCCTCCT CCCCACAGAC TGAGAAACAT
    CTGAATGGAC CCTACTTACA GCAAGACTCC TCTGCGGCTC TCGGCTATCC GGCCAGGGAG
    ACGCAGGAAG ACAGCCTAGG GAGCCCCGTT CTGCACCACG CTTTGGGGTC CAAGCTTAGC
    CTGCAGAGCC TTAGCAACTC GTTCAACTCT TCCTACATGA GCAGCGATAA TGATTCTGAC
    ATAGAAGATG AAGATCTGAA GAAGGAGCTG CGAAGGCTAC GAGACAAGCA TCTCAAGGAG
    ATTCAGGAAC TGCAGAGTCG ACAGAAGCAG GAGATCGAGC TGTTGTACAC GGCTTTGGGA
    AAAGTGCCCC CTGCTGTCAT TATCCCTCCG GCAGCGCCCC TTTCTGGCCG GCGCAGGAGG
    CATACCAAGG GGAAGGGCAG CAAGTCCAGC CGGGGCAGCT CCCACAACAG CCGCAGCCCT
    CATTTGTCAG GCGACCTGTC CACGCAGAGC GCTCCGTCGG CATTACCCCC CCAGCCGGGC
    CTTCTGCTCC CCCCAAGCAG TGCGCCCGAA CATGCTCATC TTCTCAAGCC CTCCCCTTCC
    AGCGACATTC TCTGCTCAGC CTTCACTAGC GACGCTGCCC TCTCTGTGCC CAGCCTGTCC
    ACCCAGGGCC AAGGCTGGGC CAAGTTTAAC TGTGAGTCAG AGCGGGTATC CTTCAAAATG
    GCTGGCAGGA GGACGCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA
    AAAGTCTGTC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CTAACACAGT CGCACCATCA
    ACTGCCCCGG CCCCCCAGCC AAACGTGCCC CCCGTGTCAT CCAGCCGCAA GGGAACCTTC
    ACGGACGATC TACACAAACT GGTGGACAAC TGGGCCCGAG ACGCCATGAA CCTGACTGGA
    AAAAAGGCTT GTAAGGCCTC TGGGCAACCC AGCTATGAGG GACCCGGGAT GGCCCGTAAG
    TTCTCTGCTC CCGGACAGCT GTGTGTCCCC ATGAACCCAT CCCTGGGGGG CTCCGTCCCT
    CTCCCAGCAG CTTCGGCGAC CTCCCTGGCT CACTACCCCA AAGGAATGTG CCCCCCGCAA
    CAATACGGAA TCCCCGGGGC GCTGTTCCAA GCTCCATGGA GTGGCACCGG CGTGCCCCCC
    TCCTCTCAGC CCCTAAGCCA GTTCCCCCCG GCGGTGACAT CTTCCTTACA GAACTTCGGT
    ATTAGCAGCT TGCAGAAATC TATCAGCAAC CCCCAAGGAT CCAACCTGCG GACTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54266
    Clone ID Related Accession (Same CDS sequence) XM_031898625.1
    Accession Version XM_031898625.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCATCGTCC ATGGTCCATT CAGCACTCCC AGACTCTCCG
    CTTTTTTTCC CAACCATTCC CAACCAGCGC CCCATGTCTT TCTCTCCGCC GCCTACTGGC
    TTGTCCCCAA AGGTGCCTGC AACCCAGCGA CGCAAGAGCA CCTCTTTCTT GGAGTCCCAA
    GGCCGCCAGG GGCTATCTCA GATTAGGACA CTCGGGCCAG GTGAGGAGAC CCAGTGCTGG
    AGGGCAGACT ACATGGCAGC TCTAGGTAAA GCATCTGTCC GATCCGAGGA TGCTCACAAG
    CATCAAAACT ATGAGGGATT GGCGTTCCAT GACGCAAGAC ATGTTCCGAC AGTTCCAAAT
    GAAACTCTCT TAAAGCCTGG GGAAGCATTG TATCTAGCCA GTCTGCCCTC ACAGGAGAGC
    TACAGCAGCA TGGCTGCCCC TGAGCATATG GAGGGCCACA AGCTGACCCA GCTTGGCTCG
    TTTTACGATT ATCACACTGG GCAAACCTAC ATTGCGCGGC CCCTGAAGAG CCTAAGACTG
    GAATCTGGGT TAAACCAGCC GTCGGCTTTG GGTAGCATCT CCAACCCAAT CTCCTCCGAC
    CCACACGCCA TGTTTCATCA GATGTTTGTG CCCCAGTCTG CTCCTCCAGT GCTGGCTCAA
    AGCAGCGAAG GCCACCCAGG GTGTGTGTTT GAGTTCCACG TGCACCCATC TGGGTCTGTT
    CCCGGCGAAG CAACCGTTTA CTTGCCACAC CGAGTTTACC GTCCTCGACG GGGCTCTGTA
    GACTTTAACC CAGAAGAACA GCCGCCGGGT TCTACGCTGC AGAGCCGCCT CCAAACTGTG
    ACTGAAGAGC AGTGCCACCA CAATGGCCCA GAACCACTCC CACCCTTTAT AGGATTCATC
    ACATGCAACC GTATGGAGAG CAGCCCCGAC TATTCCAGTG ATTCTTCCCA AAGGAATTCA
    TCCGATCCCG ATTATCTCTC TCCTCCTCTG GCCTTCAGCT CCAATATCTC TATGGCTTCC
    AAGCAGAAAA CGTTGCAGGA TCCACAGATT TTCTTTTGTG TCCCACAGTC CGGAACGACG
    GCTTATGCTG GACCATCAGC TTTGTACAGC CAGCAGCCAC AAATATCATC TGGACAACCA
    CCCACCACAT CCATGGGAAT CAGTGCTCAG CCCGGTCCCC ACACCCAGGG CATGGTGCAT
    CATCAGCCTT CTCTGCATCC AGCCTCGCAG TACCAAGTGC AGCAGCCAAT GCCTACTGTG
    GGTTCCAGTA TTGCGGCCCC ATCACAGCCG GTGATTACCC AAACGCCAGC CCCTCAGCCG
    GGCACCCAGC TCCCTGCCTC CCAGCCTTTG CCTGCTGTTC AAGGAGAGCC TCAGATTCCT
    GCTCAGACAG CTCCTGCTCC CCAGCCCGTT CCTACAGTGC CGGCCTCTCT GCCACCTCAT
    TTTCTTCCCT TGGCACAATC TTTGGTTGCA GTCCCTCTCA TTCCTCCATT CGCTGCCCCA
    CAGTTGCCTT TAACGGTACC ACCCCCGGTC ATACAGACAG GGTTCCCTTC CCTACCCATG
    ACTGTAACCC CAAGTATTGG CCAAACTGTA CCAGGAATAC CCCCATCCTT AGGAGCGCCA
    GTCCTGCCCC ATGGGCAGGC AGTGTTAGTT CCCACTCACA TCACTAATGT TGCTCTCCAG
    CCTTTGGCAC CAATCCCCAC ACCTCCTCTG TGCCCTGTAA CTCAGCCCAT TGGATTACCG
    GCCACCCTGG GAAAAGCTGC TGAGCCCTTG CTTCCTCCGG GAGATACTTC TTACCAGGTT
    TTCCAGGCTA CTCACCCTCT ATTCCCTGGA GATGTAAGCT TTGTTCCTCC CGTTGCCATC
    CCCTCAGCAA TGGTATCTCC CCCTATCCGT GGTGAAAGTT TAGCCCAACC TGGCTTCCAT
    ACTCAGCCTT ATCCTGAAAA CAGCAATTTG TATTCCCATG GAGGGCCCAC TCCCCAGCCC
    GCTGTTCTCA CTACCCAGCC GGTGCCTGCA GGAACCCAGA CGCAACCTGC CGTCGAGGGT
    TCACAGAGTA GCTCCCTGGC AATTTCCCAG GATACCCAGT CATCTCCTCA AGCAACACAA
    ACTGCCTCCT CCATGGACAG CGCTCATTCA GACGTGGCAT CAGGACTGAG TGACGGAAAC
    GAGGGCAGCA CAGGTGGGAG ACACGAAGGC CGAGCCACTA AACGACACTA CCGAAAATCT
    GTGAGAAGCC GCTCCAGGCA CGAGAAAACT GCACGCCCAA AGCTTCGAAT CCTTAATGTT
    TCCAACAAAG GGGATCGTGT TGTAGAGTGT CAGCTGGAGA CTCACAACCG CAAGATGGTC
    ACCTTCAAAT TCGACCTGGA TGGAGACAAT CCAGAGGAGA TTGCTACTAT AATGGTACAG
    AATGTGTTTA TTTTGGCTAC GGAGAGAGAA TCCTTTGTAG AGCAGGTAAA AGACATAATT
    GAGAAGGCCG ATGAAATGTT GAGCGAAGAT GTGAGCGTGG AGCCGGAAGG AGAAACGAGG
    GCAGAAAGTT TGCAAATCAA GGATGATGGG GATTACCCAG AATCTCAGAA GATGAATTCT
    GAATTTCGAA TGCCAGATCC TGTATATTCC CTACCACAGA CAGCAGGGCT TATTCCCAGC
    GTTGTCACAC AAGTCGTTCA TTCTGCCGGA AGACGATTTA TTGTCAGTCC GGTGCCTGAA
    AGCCGTCTAA AAGAACAGTC ATTCTTCAGT ACTTCATTGC AGGACCCTCA GCTACCAGTC
    TCTGTCGCGC ATGGTCCAGG GATGAATTTG TCTCATTCTG CCTCTTCTGT GAGCCTGCAG
    CAAGCCTTTG CAGAACTACG CCAGTCACAG ATGAGCGAGG GTCCTAGCAC AGCTCCTCCC
    ATATTTAACC AAACTGGACC CCCCTTTCCA CCATCCATGA CTAGTCTGGT GGCAACTCTG
    CCTTCTAATG CAATGCCTAT CCTCCAGGCA GCCCCAGTGA TCCCCAGTAG CCTGCCATCT
    TCTGTGCCTC AGCCCCTGGT GGAGGTGGTT GTACCTCAGG CTGCTGGGAT GGCTCCAACT
    AGCAATGTTC CAAGCACCTT GTCCCCTCCC CTAACTGCAG TATCCAACCA GCACGCAGAC
    ATGATACCGG GTGCTGCTGT AGCAGCTATC GGATCACTTC CTGTGACATC ACTTCCTCCT
    TTGCCCATTG TGGGTGGAGT TCCTGTAGGC ACTGCAACCC TGAGCTCACC CCTAACACCC
    CTAATCAGTT CTGTCAGTAC AATGGGGGGG ACGGTTCCCC CATCTGTCAC ATCTCCCCCA
    GTGCCACAGA TAGCGGGTAG CGTTGCCGGG AGCCTGCCTC CTGCATCAGC CTCCGCCCCT
    CTTATTCATG GCAGTGTTGC CCCATCTCAG GTACCGCTTT GCAGCAGCAG TTCTGTTCCC
    AGCTTTGCAG AGAGCGTGGC AGTCAGCCCC CTGCTGGATA GTGGCCAAAA GGCAGCAGAA
    GTGGGCACGG TAATGAGCAC GACTGGCTTG TCTCTGCCCA TCTCTGCACC TCTGACATAT
    GCTGGGGTGG TTGCAGCATC AGGCATCACA AGTCAACCTG CCGTGCATCT CCCCCAGGGG
    GTGGCCACAA CACCAGTTGT ATCCCAGGCA GTAGGGGGAG CTGTGGGCAC ACCCCTCTCA
    TCTCTTCCTC CCATCCCTGG AGGCATGTCT GTTAGCCAAC CAGGATCAAA TATACCCCCT
    GTGCAACACA CGTTGGCCCA TGGCCAACCC CAGCCTACCC CTCTCCCCAA CCAGCCTCAT
    ACGCACCTTG TAGAGGGTGA CACCGATAAC CTGCCCAAAC CTGGCATAGA CGATATAAAA
    ACCCTGGAGG AGAAGCTGAG GTCGCTGTTC AGTGAGCATG GCAGTGCCGG GGCCCCTCAT
    GGACTCACCC CATCAGAAAC TCAATTGGGT TTGGATGGGG CCCCCCTGCC AGGCCCTTCC
    ACAGGTAGCA CCGCTCAGAC CAAGCCTGCA GCATCCTCTT CTCCACCAAC CAGCTTACCT
    TTAGGGCCAC CTGGAGTGAC GACAATGACT CAGATCATGA CACCTGGACA AGCTGCTACA
    CCTGTCACCT ACGTATCTGC AGCTGGAAGC TCTATAGCCA CGGCCAAAGC TGCCACCCCA
    CCCTCAAAGC CGCCTCTCAG TCGGGTACCG GTGACCACGG CCGGGGCAGA GACCCCAGCA
    GGCAGTCTCC CTGGTGAACA CCTGCCTCCT CCTTCTCTCC CAGGACCTTC ACTAACTCAG
    TCCCAGCAGC CTCTAGAAGA CCTGGATTTC CAGCTGAGAA GGACGTTGAG CCCTGACACT
    GTCCCTGTGC CTTCTGCACT AATATTTCCT GCACACAGCG GAGCTTCTAC CGGTGTTGCA
    GCACCCGTCG GCTCAGCATT GCAACCCTCC GCTTCAGAAT TGCCAAAAGC CAGCGCTGGA
    GAGGCAGAGG TGGGCGCGAG TGTCCAGAGG ATGGGCAGGT TTCAGGTCTC TTTGGCAGCA
    GAGAATGTGC TCAAGCAGAG CGAGGAGACC AAACTGTCCA TCTCAGACAC TTCCTCCACC
    ACTTCTACAT CCTCCTCTTC CTCTGCGTCA TGCAGCAGCC CAGAGAGCAC CCTGACTAAG
    CCCCAGGCCA AAACCGCTGC TCCCTTAGAC ATAGTGGATG GTGTTGGTCC CCACTTGGTG
    CATCTACAGA GCCTATCTCC CAAGCAGTTG GTGAAAGTGG GGCGCTTCCA GGTTACCACT
    GCCACCACGG ACAAAGTGGG GCGCTTTTCT GTGTCCCGGA CACAAGATGA TATATCTGCT
    GGGGTGCTGG AGACAGACTC CCAGAGGTCC TCCTCCCCAC AGACTGAGAA ACATCTGAAT
    GGACCCTACT TACAGCAAGA CTCCTCTGCG GCTCTCGGCT ATCCGGCCAG GGAGACGCAG
    GAAGACAGCC TAGGGAGCCC CGTTCTGCAC CACGCTTTGG GGTCCAAGCT TAGCCTGCAG
    AGCCTTAGCA ACTCGTTCAA CTCTTCCTAC ATGAGCAGCG ATAATGATTC TGACATAGAA
    GATGAAGATC TGAAGAAGGA GCTGCGAAGG CTACGAGACA AGCATCTCAA GGAGATTCAG
    GAACTGCAGA GTCGACAGAA GCAGGAGATC GAGCTGTTGT ACACGGCTTT GGGAAAAGTG
    CCCCCTGCTG TCATTATCCC TCCGGCAGCG CCCCTTTCTG GCCGGCGCAG GAGGCATACC
    AAGGGGAAGG GCAGCAAGTC CAGCCGGGGC AGCTCCCACA ACAGCCGCAG CCCTCATTTG
    TCAGGCGACC TGTCCACGCA GAGCGCTCCG TCGGCATTAC CCCCCCAGCC GGGCCTTCTG
    CTCCCCCCAA GCAGTGCGCC CGAACATGCT CATCTTCTCA AGCCCTCCCC TTCCAGCGAC
    ATTCTCTGCT CAGCCTTCAC TAGCGACGCT GCCCTCTCTG TGCCCAGCCT GTCCACCCAG
    GGCCAAGGCT GGGCCAAGTT TAACTGTGAG TCAGAGCGGG TATCCTTCAA AATGGCTGGC
    AGGAGGACGC GATTTCTGAG TACGCCCTGC TTGGCTCTTT GGAAGATGGT GAAAAAAGTC
    TGTCCTTGCA ACCAGCTCTG TAGGACCAGC AGCACTAACA CAGTCGCACC ATCAACTGCC
    CCGGCCCCCC AGCCAAACGT GCCCCCCGTG TCATCCAGCC GCAAGGGAAC CTTCACGGAC
    GATCTACACA AACTGGTGGA CAACTGGGCC CGAGACGCCA TGAACCTGAC TGGAAAAAAG
    GCTTGTAAGG CCTCTGGGCA ACCCAGCTAT GAGGGACCCG GGATGGCCCG TAAGTTCTCT
    GCTCCCGGAC AGCTGTGTGT CCCCATGAAC CCATCCCTGG GGGGCTCCGT CCCTCTCCCA
    GCAGCTTCGG CGACCTCCCT GGCTCACTAC CCCAAAGGAA TGTGCCCCCC GCAACAATAC
    GGAATCCCCG GGGCGCTGTT CCAAGCTCCA TGGAGTGGCA CCGGCGTGCC CCCCTCCTCT
    CAGCCCCTAA GCCAGTTCCC CCCGGCGGTG ACATCTTCCT TACAGAACTT CGGTATTAGC
    AGCTTGCAGA AATCTATCAG CAACCCCCAA GGATCCAACC TGCGGACTAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754485.1
    CDS327..8420
    Translation

    Target ORF information:

    RefSeq Version XM_031898625.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCATCGTCC ATGGTCCATT CAGCACTCCC AGACTCTCCG
    CTTTTTTTCC CAACCATTCC CAACCAGCGC CCCATGTCTT TCTCTCCGCC GCCTACTGGC
    TTGTCCCCAA AGGTGCCTGC AACCCAGCGA CGCAAGAGCA CCTCTTTCTT GGAGTCCCAA
    GGCCGCCAGG GGCTATCTCA GATTAGGACA CTCGGGCCAG GTGAGGAGAC CCAGTGCTGG
    AGGGCAGACT ACATGGCAGC TCTAGGTAAA GCATCTGTCC GATCCGAGGA TGCTCACAAG
    CATCAAAACT ATGAGGGATT GGCGTTCCAT GACGCAAGAC ATGTTCCGAC AGTTCCAAAT
    GAAACTCTCT TAAAGCCTGG GGAAGCATTG TATCTAGCCA GTCTGCCCTC ACAGGAGAGC
    TACAGCAGCA TGGCTGCCCC TGAGCATATG GAGGGCCACA AGCTGACCCA GCTTGGCTCG
    TTTTACGATT ATCACACTGG GCAAACCTAC ATTGCGCGGC CCCTGAAGAG CCTAAGACTG
    GAATCTGGGT TAAACCAGCC GTCGGCTTTG GGTAGCATCT CCAACCCAAT CTCCTCCGAC
    CCACACGCCA TGTTTCATCA GATGTTTGTG CCCCAGTCTG CTCCTCCAGT GCTGGCTCAA
    AGCAGCGAAG GCCACCCAGG GTGTGTGTTT GAGTTCCACG TGCACCCATC TGGGTCTGTT
    CCCGGCGAAG CAACCGTTTA CTTGCCACAC CGAGTTTACC GTCCTCGACG GGGCTCTGTA
    GACTTTAACC CAGAAGAACA GCCGCCGGGT TCTACGCTGC AGAGCCGCCT CCAAACTGTG
    ACTGAAGAGC AGTGCCACCA CAATGGCCCA GAACCACTCC CACCCTTTAT AGGATTCATC
    ACATGCAACC GTATGGAGAG CAGCCCCGAC TATTCCAGTG ATTCTTCCCA AAGGAATTCA
    TCCGATCCCG ATTATCTCTC TCCTCCTCTG GCCTTCAGCT CCAATATCTC TATGGCTTCC
    AAGCAGAAAA CGTTGCAGGA TCCACAGATT TTCTTTTGTG TCCCACAGTC CGGAACGACG
    GCTTATGCTG GACCATCAGC TTTGTACAGC CAGCAGCCAC AAATATCATC TGGACAACCA
    CCCACCACAT CCATGGGAAT CAGTGCTCAG CCCGGTCCCC ACACCCAGGG CATGGTGCAT
    CATCAGCCTT CTCTGCATCC AGCCTCGCAG TACCAAGTGC AGCAGCCAAT GCCTACTGTG
    GGTTCCAGTA TTGCGGCCCC ATCACAGCCG GTGATTACCC AAACGCCAGC CCCTCAGCCG
    GGCACCCAGC TCCCTGCCTC CCAGCCTTTG CCTGCTGTTC AAGGAGAGCC TCAGATTCCT
    GCTCAGACAG CTCCTGCTCC CCAGCCCGTT CCTACAGTGC CGGCCTCTCT GCCACCTCAT
    TTTCTTCCCT TGGCACAATC TTTGGTTGCA GTCCCTCTCA TTCCTCCATT CGCTGCCCCA
    CAGTTGCCTT TAACGGTACC ACCCCCGGTC ATACAGACAG GGTTCCCTTC CCTACCCATG
    ACTGTAACCC CAAGTATTGG CCAAACTGTA CCAGGAATAC CCCCATCCTT AGGAGCGCCA
    GTCCTGCCCC ATGGGCAGGC AGTGTTAGTT CCCACTCACA TCACTAATGT TGCTCTCCAG
    CCTTTGGCAC CAATCCCCAC ACCTCCTCTG TGCCCTGTAA CTCAGCCCAT TGGATTACCG
    GCCACCCTGG GAAAAGCTGC TGAGCCCTTG CTTCCTCCGG GAGATACTTC TTACCAGGTT
    TTCCAGGCTA CTCACCCTCT ATTCCCTGGA GATGTAAGCT TTGTTCCTCC CGTTGCCATC
    CCCTCAGCAA TGGTATCTCC CCCTATCCGT GGTGAAAGTT TAGCCCAACC TGGCTTCCAT
    ACTCAGCCTT ATCCTGAAAA CAGCAATTTG TATTCCCATG GAGGGCCCAC TCCCCAGCCC
    GCTGTTCTCA CTACCCAGCC GGTGCCTGCA GGAACCCAGA CGCAACCTGC CGTCGAGGGT
    TCACAGAGTA GCTCCCTGGC AATTTCCCAG GATACCCAGT CATCTCCTCA AGCAACACAA
    ACTGCCTCCT CCATGGACAG CGCTCATTCA GACGTGGCAT CAGGACTGAG TGACGGAAAC
    GAGGGCAGCA CAGGTGGGAG ACACGAAGGC CGAGCCACTA AACGACACTA CCGAAAATCT
    GTGAGAAGCC GCTCCAGGCA CGAGAAAACT GCACGCCCAA AGCTTCGAAT CCTTAATGTT
    TCCAACAAAG GGGATCGTGT TGTAGAGTGT CAGCTGGAGA CTCACAACCG CAAGATGGTC
    ACCTTCAAAT TCGACCTGGA TGGAGACAAT CCAGAGGAGA TTGCTACTAT AATGGTACAG
    AATGTGTTTA TTTTGGCTAC GGAGAGAGAA TCCTTTGTAG AGCAGGTAAA AGACATAATT
    GAGAAGGCCG ATGAAATGTT GAGCGAAGAT GTGAGCGTGG AGCCGGAAGG AGAAACGAGG
    GCAGAAAGTT TGCAAATCAA GGATGATGGG GATTACCCAG AATCTCAGAA GATGAATTCT
    GAATTTCGAA TGCCAGATCC TGTATATTCC CTACCACAGA CAGCAGGGCT TATTCCCAGC
    GTTGTCACAC AAGTCGTTCA TTCTGCCGGA AGACGATTTA TTGTCAGTCC GGTGCCTGAA
    AGCCGTCTAA AAGAACAGTC ATTCTTCAGT ACTTCATTGC AGGACCCTCA GCTACCAGTC
    TCTGTCGCGC ATGGTCCAGG GATGAATTTG TCTCATTCTG CCTCTTCTGT GAGCCTGCAG
    CAAGCCTTTG CAGAACTACG CCAGTCACAG ATGAGCGAGG GTCCTAGCAC AGCTCCTCCC
    ATATTTAACC AAACTGGACC CCCCTTTCCA CCATCCATGA CTAGTCTGGT GGCAACTCTG
    CCTTCTAATG CAATGCCTAT CCTCCAGGCA GCCCCAGTGA TCCCCAGTAG CCTGCCATCT
    TCTGTGCCTC AGCCCCTGGT GGAGGTGGTT GTACCTCAGG CTGCTGGGAT GGCTCCAACT
    AGCAATGTTC CAAGCACCTT GTCCCCTCCC CTAACTGCAG TATCCAACCA GCACGCAGAC
    ATGATACCGG GTGCTGCTGT AGCAGCTATC GGATCACTTC CTGTGACATC ACTTCCTCCT
    TTGCCCATTG TGGGTGGAGT TCCTGTAGGC ACTGCAACCC TGAGCTCACC CCTAACACCC
    CTAATCAGTT CTGTCAGTAC AATGGGGGGG ACGGTTCCCC CATCTGTCAC ATCTCCCCCA
    GTGCCACAGA TAGCGGGTAG CGTTGCCGGG AGCCTGCCTC CTGCATCAGC CTCCGCCCCT
    CTTATTCATG GCAGTGTTGC CCCATCTCAG GTACCGCTTT GCAGCAGCAG TTCTGTTCCC
    AGCTTTGCAG AGAGCGTGGC AGTCAGCCCC CTGCTGGATA GTGGCCAAAA GGCAGCAGAA
    GTGGGCACGG TAATGAGCAC GACTGGCTTG TCTCTGCCCA TCTCTGCACC TCTGACATAT
    GCTGGGGTGG TTGCAGCATC AGGCATCACA AGTCAACCTG CCGTGCATCT CCCCCAGGGG
    GTGGCCACAA CACCAGTTGT ATCCCAGGCA GTAGGGGGAG CTGTGGGCAC ACCCCTCTCA
    TCTCTTCCTC CCATCCCTGG AGGCATGTCT GTTAGCCAAC CAGGATCAAA TATACCCCCT
    GTGCAACACA CGTTGGCCCA TGGCCAACCC CAGCCTACCC CTCTCCCCAA CCAGCCTCAT
    ACGCACCTTG TAGAGGGTGA CACCGATAAC CTGCCCAAAC CTGGCATAGA CGATATAAAA
    ACCCTGGAGG AGAAGCTGAG GTCGCTGTTC AGTGAGCATG GCAGTGCCGG GGCCCCTCAT
    GGACTCACCC CATCAGAAAC TCAATTGGGT TTGGATGGGG CCCCCCTGCC AGGCCCTTCC
    ACAGGTAGCA CCGCTCAGAC CAAGCCTGCA GCATCCTCTT CTCCACCAAC CAGCTTACCT
    TTAGGGCCAC CTGGAGTGAC GACAATGACT CAGATCATGA CACCTGGACA AGCTGCTACA
    CCTGTCACCT ACGTATCTGC AGCTGGAAGC TCTATAGCCA CGGCCAAAGC TGCCACCCCA
    CCCTCAAAGC CGCCTCTCAG TCGGGTACCG GTGACCACGG CCGGGGCAGA GACCCCAGCA
    GGCAGTCTCC CTGGTGAACA CCTGCCTCCT CCTTCTCTCC CAGGACCTTC ACTAACTCAG
    TCCCAGCAGC CTCTAGAAGA CCTGGATTTC CAGCTGAGAA GGACGTTGAG CCCTGACACT
    GTCCCTGTGC CTTCTGCACT AATATTTCCT GCACACAGCG GAGCTTCTAC CGGTGTTGCA
    GCACCCGTCG GCTCAGCATT GCAACCCTCC GCTTCAGAAT TGCCAAAAGC CAGCGCTGGA
    GAGGCAGAGG TGGGCGCGAG TGTCCAGAGG ATGGGCAGGT TTCAGGTCTC TTTGGCAGCA
    GAGAATGTGC TCAAGCAGAG CGAGGAGACC AAACTGTCCA TCTCAGACAC TTCCTCCACC
    ACTTCTACAT CCTCCTCTTC CTCTGCGTCA TGCAGCAGCC CAGAGAGCAC CCTGACTAAG
    CCCCAGGCCA AAACCGCTGC TCCCTTAGAC ATAGTGGATG GTGTTGGTCC CCACTTGGTG
    CATCTACAGA GCCTATCTCC CAAGCAGTTG GTGAAAGTGG GGCGCTTCCA GGTTACCACT
    GCCACCACGG ACAAAGTGGG GCGCTTTTCT GTGTCCCGGA CACAAGATGA TATATCTGCT
    GGGGTGCTGG AGACAGACTC CCAGAGGTCC TCCTCCCCAC AGACTGAGAA ACATCTGAAT
    GGACCCTACT TACAGCAAGA CTCCTCTGCG GCTCTCGGCT ATCCGGCCAG GGAGACGCAG
    GAAGACAGCC TAGGGAGCCC CGTTCTGCAC CACGCTTTGG GGTCCAAGCT TAGCCTGCAG
    AGCCTTAGCA ACTCGTTCAA CTCTTCCTAC ATGAGCAGCG ATAATGATTC TGACATAGAA
    GATGAAGATC TGAAGAAGGA GCTGCGAAGG CTACGAGACA AGCATCTCAA GGAGATTCAG
    GAACTGCAGA GTCGACAGAA GCAGGAGATC GAGCTGTTGT ACACGGCTTT GGGAAAAGTG
    CCCCCTGCTG TCATTATCCC TCCGGCAGCG CCCCTTTCTG GCCGGCGCAG GAGGCATACC
    AAGGGGAAGG GCAGCAAGTC CAGCCGGGGC AGCTCCCACA ACAGCCGCAG CCCTCATTTG
    TCAGGCGACC TGTCCACGCA GAGCGCTCCG TCGGCATTAC CCCCCCAGCC GGGCCTTCTG
    CTCCCCCCAA GCAGTGCGCC CGAACATGCT CATCTTCTCA AGCCCTCCCC TTCCAGCGAC
    ATTCTCTGCT CAGCCTTCAC TAGCGACGCT GCCCTCTCTG TGCCCAGCCT GTCCACCCAG
    GGCCAAGGCT GGGCCAAGTT TAACTGTGAG TCAGAGCGGG TATCCTTCAA AATGGCTGGC
    AGGAGGACGC GATTTCTGAG TACGCCCTGC TTGGCTCTTT GGAAGATGGT GAAAAAAGTC
    TGTCCTTGCA ACCAGCTCTG TAGGACCAGC AGCACTAACA CAGTCGCACC ATCAACTGCC
    CCGGCCCCCC AGCCAAACGT GCCCCCCGTG TCATCCAGCC GCAAGGGAAC CTTCACGGAC
    GATCTACACA AACTGGTGGA CAACTGGGCC CGAGACGCCA TGAACCTGAC TGGAAAAAAG
    GCTTGTAAGG CCTCTGGGCA ACCCAGCTAT GAGGGACCCG GGATGGCCCG TAAGTTCTCT
    GCTCCCGGAC AGCTGTGTGT CCCCATGAAC CCATCCCTGG GGGGCTCCGT CCCTCTCCCA
    GCAGCTTCGG CGACCTCCCT GGCTCACTAC CCCAAAGGAA TGTGCCCCCC GCAACAATAC
    GGAATCCCCG GGGCGCTGTT CCAAGCTCCA TGGAGTGGCA CCGGCGTGCC CCCCTCCTCT
    CAGCCCCTAA GCCAGTTCCC CCCGGCGGTG ACATCTTCCT TACAGAACTT CGGTATTAGC
    AGCTTGCAGA AATCTATCAG CAACCCCCAA GGATCCAACC TGCGGACTAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54271
    Clone ID Related Accession (Same CDS sequence) XM_031898631.1
    Accession Version XM_031898631.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    901
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    4141
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    6301
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    7381
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCTTGTCGA GGTCGTAGCA TGTCAGTATG TGTTCCCAAC
    CTTTCCTCTG CTCCCCCACT CCTTTCCGTT GTGCCCCCCA GTGCTCCAGG CTCCCCTTCC
    CCATTGCAGT CTGCCCCTGC CTCCCCACTC TGGACATCTC CCGAAGATTC CTTTGCTGAA
    AAGCTTTCCA AGGCTCTAGA GAGTGTTCTG CCCATGCACT CCTCCAGCCC TCGCAAGCAC
    CAACGCCGCT GCAGCCTGCC TACCCTCTAT ACCAGCCCTC CATCGTCCAT GGTCCATTCA
    GCACTCCCAG ACTCTCCGCT TTTTTTCCCA ACCATTCCCA ACCAGCGCCC CATGTCTTTC
    TCTCCGCCGC CTACTGGCTT GTCCCCAAAG GTGCCTGCAA CCCAGCGACG CAAGAGCACC
    TCTTTCTTGG AGTCCCAAGG CCGCCAGGGG CTATCTCAGA TTAGGACACT CGGGCCAGGT
    GAGGAGACCC AGTGCTGGAG GGCAGACTAC ATGGCAGCTC TAGGTAAAGC ATCTGTCCGA
    TCCGAGGATG CTCACAAGCA TCAAAACTAT GAGGGATTGG CGTTCCATGA CGCAAGACAT
    GTTCCGACAG TTCCAAATGA AACTCTCTTA AAGCCTGGGG AAGCATTGTA TCTAGCCAGT
    CTGCCCTCAC AGGAGAGCTA CAGCAGCATG GCTGCCCCTG AGCATATGGA GGGCCACAAG
    CTGACCCAGC TTGGCTCGTT TTACGATTAT CACACTGGGC AAACCTACAT TGCGCGGCCC
    CTGAAGAGCC TAAGACTGGA ATCTGGGTTA AACCAGCCGT CGGCTTTGGG TAGCATCTCC
    AACCCAATCT CCTCCGACCC ACACGCCATG TTTCATCAGA TGTTTGTGCC CCAGTCTGCT
    CCTCCAGTGC TGGCTCAAAG CAGCGAAGGC CACCCAGGGT GTGTGTTTGA GTTCCACGTG
    CACCCATCTG GGTCTGTTCC CGGCGAAGCA ACCGTTTACT TGCCACACCG AGTTTACCGT
    CCTCGACGGG GCTCTGTAGA CTTTAACCCA GAAGAACAGC CGCCGGGTTC TACGCTGCAG
    AGCCGCCTCC AAACTGTGAC TGAAGAGCAG TGCCACCACA ATGGCCCAGA ACCACTCCCA
    CCCTTTATAG GATTCATCAC ATGCAACCGT ATGGAGAGCA GCCCCGACTA TTCCAGTGAT
    TCTTCCCAAA GGAATTCATC CGATCCCGAT TATCTCTCTC CTCCTCTGGC CTTCAGCTCC
    AATATCTCTA TGGCTTCCAA GCAGAAAACG TTGCAGGATC CACAGATTTT CTTTTGTGTC
    CCACAGTCCG GAACGACGGC TTATGCTGGA CCATCAGCTT TGTACAGCCA GCAGCCACAA
    ATATCATCTG GACAACCACC CACCACATCC ATGGGAATCA GTGCTCAGCC CGGTCCCCAC
    ACCCAGGGCA TGGTGCATCA TCAGCCTTCT CTGCATCCAG CCTCGCAGTA CCAAGTGCAG
    CAGCCAATGC CTACTGTGGG TTCCAGTATT GCGGCCCCAT CACAGCCGGT GATTACCCAA
    ACGCCAGCCC CTCAGCCGGG CACCCAGCTC CCTGCCTCCC AGCCTTTGCC TGCTGTTCAA
    GGAGAGCCTC AGATTCCTGC TCAGACAGCT CCTGCTCCCC AGCCCGTTCC TACAGTGCCG
    GCCTCTCTGC CACCTCATTT TCTTCCCTTG GCACAATCTT TGGTTGCAGT CCCTCTCATT
    CCTCCATTCG CTGCCCCACA GTTGCCTTTA ACGGTACCAC CCCCGGTCAT ACAGACAGGG
    TTCCCTTCCC TACCCATGAC TGTAACCCCA AGTATTGGCC AAACTGTACC AGGAATACCC
    CCATCCTTAG GAGCGCCAGT CCTGCCCCAT GGGCAGGCAG TGTTAGTTCC CACTCACATC
    ACTAATGTTG CTCTCCAGCC TTTGGCACCA ATCCCCACAC CTCCTCTGTG CCCTGTAACT
    CAGCCCATTG GATTACCGGC CACCCTGGGA AAAGCTGCTG AGCCCTTGCT TCCTCCGGGA
    GATACTTCTT ACCAGGTTTT CCAGGCTACT CACCCTCTAT TCCCTGGAGA TGTAAGCTTT
    GTTCCTCCCG TTGCCATCCC CTCAGCAATG GTATCTCCCC CTATCCGTGG TGAAAGTTTA
    GCCCAACCTG GCTTCCATAC TCAGCCTTAT CCTGAAAACA GCAATTTGTA TTCCCATGGA
    GGGCCCACTC CCCAGCCCGC TGTTCTCACT ACCCAGCCGG TGCCTGCAGG AACCCAGACG
    CAACCTGCCG TCGAGGGTTC ACAGAGTAGC TCCCTGGCAA TTTCCCAGGA TACCCAGTCA
    TCTCCTCAAG CAACACAAAC TGCCTCCTCC ATGGACAGCG CTCATTCAGA CGTGGCATCA
    GGACTGAGTG ACGGAAACGA GGGCAGCACA GGTGGGAGAC ACGAAGGCCG AGCCACTAAA
    CGACACTACC GAAAATCTGT GAGAAGCCGC TCCAGGCACG AGAAAACTGC ACGCCCAAAG
    CTTCGAATCC TTAATGTTTC CAACAAAGGG GATCGTGTTG TAGAGTGTCA GCTGGAGACT
    CACAACCGCA AGATGGTCAC CTTCAAATTC GACCTGGATG GAGACAATCC AGAGGAGATT
    GCTACTATAA TGGTACAGAA TGTGTTTATT TTGGCTACGG AGAGAGAATC CTTTGTAGAG
    CAGGTAAAAG ACATAATTGA GAAGGCCGAT GAAATGTTGA GCGAAGATGT GAGCGTGGAG
    CCGGAAGGAG AAACGAGGGC AGAAAGTTTG CAAATCAAGG ATGATGGGGA TTACCCAGAA
    TCTCAGAAGA TGAATTCTGA ATTTCGAATG CCAGATCCTG TATATTCCCT ACCACAGACA
    GCAGGGCTTA TTCCCAGCGT TGTCACACAA GTCGTTCATT CTGCCGGAAG ACGATTTATT
    GTCAGTCCGG TGCCTGAAAG CCGTCTAAAA GAACAGTCAT TCTTCAGTAC TTCATTGCAG
    GACCCTCAGC TACCAGTCTC TGTCGCGCAT GGTCCAGGGA TGAATTTGTC TCATTCTGCC
    TCTTCTGTGA GCCTGCAGCA AGCCTTTGCA GAACTACGCC AGTCACAGAT GAGCGAGGGT
    CCTAGCACAG CTCCTCCCAT ATTTAACCAA ACTGGACCCC CCTTTCCACC ATCCATGACT
    AGTCTGGTGG CAACTCTGCC TTCTAATGCA ATGCCTATCC TCCAGGCAGC CCCAGTGATC
    CCCAGTAGCC TGCCATCTTC TGTGCCTCAG CCCCTGGTGG AGGTGGTTGT ACCTCAGGCT
    GCTGGGATGG CTCCAACTAG CAATGTTCCA AGCACCTTGT CCCCTCCCCT AACTGCAGTA
    TCCAACCAGC ACGCAGACAT GATACCGGGT GCTGCTGTAG CAGCTATCGG ATCACTTCCT
    GTGACATCAC TTCCTCCTTT GCCCATTGTG GGTGGAGTTC CTGTAGGCAC TGCAACCCTG
    AGCTCACCCC TAACACCCCT AATCAGTTCT GTCAGTACAA TGGGGGGGAC GGTTCCCCCA
    TCTGTCACAT CTCCCCCAGT GCCACAGATA GCGGGTAGCG TTGCCGGGAG CCTGCCTCCT
    GCATCAGCCT CCGCCCCTCT TATTCATGGC AGTGTTGCCC CATCTCAGGT ACCGCTTTGC
    AGCAGCAGTT CTGTTCCCAG CTTTGCAGAG AGCGTGGCAG TCAGCCCCCT GCTGGATAGT
    GGCCAAAAGG CAGCAGAAGT GGGCACGGTA ATGAGCACGA CTGGCTTGTC TCTGCCCATC
    TCTGCACCTC TGACATATGC TGGGGTGGTT GCAGCATCAG GCATCACAAG TCAACCTGCC
    GTGCATCTCC CCCAGGGGGT GGCCACAACA CCAGTTGTAT CCCAGGCAGT AGGGGGAGCT
    GTGGGCACAC CCCTCTCATC TCTTCCTCCC ATCCCTGGAG GCATGTCTGT TAGCCAACCA
    GGATCAAATA TACCCCCTGT GCAACACACG TTGGCCCATG GCCAACCCCA GCCTACCCCT
    CTCCCCAACC AGCCTCATAC GCACCTTGTA GAGGGTGACA CCGATAACCT GCCCAAACCT
    GGCATAGACG ATATAAAAAC CCTGGAGGAG AAGCTGAGGT CGCTGTTCAG TGAGCATGGC
    AGTGCCGGGG CCCCTCATGG ACTCACCCCA TCAGAAACTC AATTGGGTTT GGATGGGGCC
    CCCCTGCCAG GCCCTTCCAC AGGTAGCACC GCTCAGACCA AGCCTGCAGC ATCCTCTTCT
    CCACCAACCA GCTTACCTTT AGGGCCACCT GGAGTGACGA CAATGACTCA GATCATGACA
    CCTGGACAAG CTGCTACACC TGTCACCTAC GTATCTGCAG CTGGAAGCTC TATAGCCACG
    GCCAAAGCTG CCACCCCACC CTCAAAGCCG CCTCTCAGTC GGGTACCGGT GACCACGGCC
    GGGGCAGAGA CCCCAGCAGG CAGTCTCCCT GGTGAACACC TGCCTCCTCC TTCTCTCCCA
    GGACCTTCAC TAACTCAGTC CCAGCAGCCT CTAGAAGACC TGGATTTCCA GCTGAGAAGG
    ACGTTGAGCC CTGACACTGT CCCTGTGCCT TCTGCACTAA TATTTCCTGC ACACAGCGGA
    GCTTCTACCG GTGTTGCAGC ACCCGTCGGC TCAGCATTGC AACCCTCCGC TTCAGAATTG
    CCAAAAGCCA GCGCTGGAGA GGCAGAGGTG GGCGCGAGTG TCCAGAGGAT GGGCAGGTTT
    CAGGTCTCTT TGGCAGCAGA GAATGTGCTC AAGCAGAGCG AGGAGACCAA ACTGTCCATC
    TCAGACACTT CCTCCACCAC TTCTACATCC TCCTCTTCCT CTGCGTCATG CAGCAGCCCA
    GAGAGCACCC TGACTAAGCC CCAGGCCAAA ACCGCTGCTC CCTTAGACAT AGTGGATGGT
    GTTGGTCCCC ACTTGGTGCA TCTACAGAGC CTATCTCCCA AGCAGTTGGT GAAAGTGGGG
    CGCTTCCAGG TTACCACTGC CACCACGGAC AAAGTGGGGC GCTTTTCTGT GTCCCGGACA
    CAAGATGATA TATCTGCTGG GGTGCTGGAG ACAGACTCCC AGAGGTCCTC CTCCCCACAG
    ACTGAGAAAC ATCTGAATGG ACCCTACTTA CAGCAAGACT CCTCTGCGGC TCTCGGCTAT
    CCGGCCAGGG AGACGCAGGA AGACAGCCTA GGGAGCCCCG TTCTGCACCA CGCTTTGGGG
    TCCAAGCTTA GCCTGCAGAG CCTTAGCAAC TCGTTCAACT CTTCCTACAT GAGCAGCGAT
    AATGATTCTG ACATAGAAGA TGAAGATCTG AAGAAGGAGC TGCGAAGGCT ACGAGACAAG
    CATCTCAAGG AGATTCAGGA ACTGCAGAGT CGACAGAAGC AGGAGATCGA GCTGTTGTAC
    ACGGCTTTGG GAAAAGTGCC CCCTGCTGTC ATTATCCCTC CGGCAGCGCC CCTTTCTGGC
    CGGCGCAGGA GGCATACCAA GGGGAAGGGC AGCAAGTCCA GCCGGGGCAG CTCCCACAAC
    AGCCGCAGCC CTCATTTGTC AGGCGACCTG TCCACGCAGA GCGCTCCGTC GGCATTACCC
    CCCCAGCCGG GCCTTCTGCT CCCCCCAAGC AGTGCGCCCG AACATGCTCA TCTTCTCAAG
    CCCTCCCCTT CCAGCGACAT TCTCTGCTCA GCCTTCACTA GCGACGCTGC CCTCTCTGTG
    CCCAGCCTGT CCACCCAGGG CCAAGGGACC AGCAGCACTA ACACAGTCGC ACCATCAACT
    GCCCCGGCCC CCCAGCCAAA CGTGCCCCCC GTGTCATCCA GCCGCAAGGG AACCTTCACG
    GACGATCTAC ACAAACTGGT GGACAACTGG GCCCGAGACG CCATGAACCT GACTGGAAAA
    AAGGCTTGTA AGGCCTCTGG GCAACCCAGC TATGAGGGAC CCGGGATGGC CCGTAAGTTC
    TCTGCTCCCG GACAGCTGTG TGTCCCCATG AACCCATCCC TGGGGGGCTC CGTCCCTCTC
    CCAGCAGCTT CGGCGACCTC CCTGGCTCAC TACCCCAAAG GAATGTGCCC CCCGCAACAA
    TACGGAATCC CCGGGGCGCT GTTCCAAGCT CCATGGAGTG GCACCGGCGT GCCCCCCTCC
    TCTCAGCCCC TAAGCCAGTT CCCCCCGGCG GTGACATCTT CCTTACAGAA CTTCGGTATT
    AGCAGCTTGC AGAAATCTAT CAGCAACCCC CAAGGATCCA ACCTGCGGAC TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754491.1
    CDS327..8543
    Translation

    Target ORF information:

    RefSeq Version XM_031898631.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCTTGTCGA GGTCGTAGCA TGTCAGTATG TGTTCCCAAC
    CTTTCCTCTG CTCCCCCACT CCTTTCCGTT GTGCCCCCCA GTGCTCCAGG CTCCCCTTCC
    CCATTGCAGT CTGCCCCTGC CTCCCCACTC TGGACATCTC CCGAAGATTC CTTTGCTGAA
    AAGCTTTCCA AGGCTCTAGA GAGTGTTCTG CCCATGCACT CCTCCAGCCC TCGCAAGCAC
    CAACGCCGCT GCAGCCTGCC TACCCTCTAT ACCAGCCCTC CATCGTCCAT GGTCCATTCA
    GCACTCCCAG ACTCTCCGCT TTTTTTCCCA ACCATTCCCA ACCAGCGCCC CATGTCTTTC
    TCTCCGCCGC CTACTGGCTT GTCCCCAAAG GTGCCTGCAA CCCAGCGACG CAAGAGCACC
    TCTTTCTTGG AGTCCCAAGG CCGCCAGGGG CTATCTCAGA TTAGGACACT CGGGCCAGGT
    GAGGAGACCC AGTGCTGGAG GGCAGACTAC ATGGCAGCTC TAGGTAAAGC ATCTGTCCGA
    TCCGAGGATG CTCACAAGCA TCAAAACTAT GAGGGATTGG CGTTCCATGA CGCAAGACAT
    GTTCCGACAG TTCCAAATGA AACTCTCTTA AAGCCTGGGG AAGCATTGTA TCTAGCCAGT
    CTGCCCTCAC AGGAGAGCTA CAGCAGCATG GCTGCCCCTG AGCATATGGA GGGCCACAAG
    CTGACCCAGC TTGGCTCGTT TTACGATTAT CACACTGGGC AAACCTACAT TGCGCGGCCC
    CTGAAGAGCC TAAGACTGGA ATCTGGGTTA AACCAGCCGT CGGCTTTGGG TAGCATCTCC
    AACCCAATCT CCTCCGACCC ACACGCCATG TTTCATCAGA TGTTTGTGCC CCAGTCTGCT
    CCTCCAGTGC TGGCTCAAAG CAGCGAAGGC CACCCAGGGT GTGTGTTTGA GTTCCACGTG
    CACCCATCTG GGTCTGTTCC CGGCGAAGCA ACCGTTTACT TGCCACACCG AGTTTACCGT
    CCTCGACGGG GCTCTGTAGA CTTTAACCCA GAAGAACAGC CGCCGGGTTC TACGCTGCAG
    AGCCGCCTCC AAACTGTGAC TGAAGAGCAG TGCCACCACA ATGGCCCAGA ACCACTCCCA
    CCCTTTATAG GATTCATCAC ATGCAACCGT ATGGAGAGCA GCCCCGACTA TTCCAGTGAT
    TCTTCCCAAA GGAATTCATC CGATCCCGAT TATCTCTCTC CTCCTCTGGC CTTCAGCTCC
    AATATCTCTA TGGCTTCCAA GCAGAAAACG TTGCAGGATC CACAGATTTT CTTTTGTGTC
    CCACAGTCCG GAACGACGGC TTATGCTGGA CCATCAGCTT TGTACAGCCA GCAGCCACAA
    ATATCATCTG GACAACCACC CACCACATCC ATGGGAATCA GTGCTCAGCC CGGTCCCCAC
    ACCCAGGGCA TGGTGCATCA TCAGCCTTCT CTGCATCCAG CCTCGCAGTA CCAAGTGCAG
    CAGCCAATGC CTACTGTGGG TTCCAGTATT GCGGCCCCAT CACAGCCGGT GATTACCCAA
    ACGCCAGCCC CTCAGCCGGG CACCCAGCTC CCTGCCTCCC AGCCTTTGCC TGCTGTTCAA
    GGAGAGCCTC AGATTCCTGC TCAGACAGCT CCTGCTCCCC AGCCCGTTCC TACAGTGCCG
    GCCTCTCTGC CACCTCATTT TCTTCCCTTG GCACAATCTT TGGTTGCAGT CCCTCTCATT
    CCTCCATTCG CTGCCCCACA GTTGCCTTTA ACGGTACCAC CCCCGGTCAT ACAGACAGGG
    TTCCCTTCCC TACCCATGAC TGTAACCCCA AGTATTGGCC AAACTGTACC AGGAATACCC
    CCATCCTTAG GAGCGCCAGT CCTGCCCCAT GGGCAGGCAG TGTTAGTTCC CACTCACATC
    ACTAATGTTG CTCTCCAGCC TTTGGCACCA ATCCCCACAC CTCCTCTGTG CCCTGTAACT
    CAGCCCATTG GATTACCGGC CACCCTGGGA AAAGCTGCTG AGCCCTTGCT TCCTCCGGGA
    GATACTTCTT ACCAGGTTTT CCAGGCTACT CACCCTCTAT TCCCTGGAGA TGTAAGCTTT
    GTTCCTCCCG TTGCCATCCC CTCAGCAATG GTATCTCCCC CTATCCGTGG TGAAAGTTTA
    GCCCAACCTG GCTTCCATAC TCAGCCTTAT CCTGAAAACA GCAATTTGTA TTCCCATGGA
    GGGCCCACTC CCCAGCCCGC TGTTCTCACT ACCCAGCCGG TGCCTGCAGG AACCCAGACG
    CAACCTGCCG TCGAGGGTTC ACAGAGTAGC TCCCTGGCAA TTTCCCAGGA TACCCAGTCA
    TCTCCTCAAG CAACACAAAC TGCCTCCTCC ATGGACAGCG CTCATTCAGA CGTGGCATCA
    GGACTGAGTG ACGGAAACGA GGGCAGCACA GGTGGGAGAC ACGAAGGCCG AGCCACTAAA
    CGACACTACC GAAAATCTGT GAGAAGCCGC TCCAGGCACG AGAAAACTGC ACGCCCAAAG
    CTTCGAATCC TTAATGTTTC CAACAAAGGG GATCGTGTTG TAGAGTGTCA GCTGGAGACT
    CACAACCGCA AGATGGTCAC CTTCAAATTC GACCTGGATG GAGACAATCC AGAGGAGATT
    GCTACTATAA TGGTACAGAA TGTGTTTATT TTGGCTACGG AGAGAGAATC CTTTGTAGAG
    CAGGTAAAAG ACATAATTGA GAAGGCCGAT GAAATGTTGA GCGAAGATGT GAGCGTGGAG
    CCGGAAGGAG AAACGAGGGC AGAAAGTTTG CAAATCAAGG ATGATGGGGA TTACCCAGAA
    TCTCAGAAGA TGAATTCTGA ATTTCGAATG CCAGATCCTG TATATTCCCT ACCACAGACA
    GCAGGGCTTA TTCCCAGCGT TGTCACACAA GTCGTTCATT CTGCCGGAAG ACGATTTATT
    GTCAGTCCGG TGCCTGAAAG CCGTCTAAAA GAACAGTCAT TCTTCAGTAC TTCATTGCAG
    GACCCTCAGC TACCAGTCTC TGTCGCGCAT GGTCCAGGGA TGAATTTGTC TCATTCTGCC
    TCTTCTGTGA GCCTGCAGCA AGCCTTTGCA GAACTACGCC AGTCACAGAT GAGCGAGGGT
    CCTAGCACAG CTCCTCCCAT ATTTAACCAA ACTGGACCCC CCTTTCCACC ATCCATGACT
    AGTCTGGTGG CAACTCTGCC TTCTAATGCA ATGCCTATCC TCCAGGCAGC CCCAGTGATC
    CCCAGTAGCC TGCCATCTTC TGTGCCTCAG CCCCTGGTGG AGGTGGTTGT ACCTCAGGCT
    GCTGGGATGG CTCCAACTAG CAATGTTCCA AGCACCTTGT CCCCTCCCCT AACTGCAGTA
    TCCAACCAGC ACGCAGACAT GATACCGGGT GCTGCTGTAG CAGCTATCGG ATCACTTCCT
    GTGACATCAC TTCCTCCTTT GCCCATTGTG GGTGGAGTTC CTGTAGGCAC TGCAACCCTG
    AGCTCACCCC TAACACCCCT AATCAGTTCT GTCAGTACAA TGGGGGGGAC GGTTCCCCCA
    TCTGTCACAT CTCCCCCAGT GCCACAGATA GCGGGTAGCG TTGCCGGGAG CCTGCCTCCT
    GCATCAGCCT CCGCCCCTCT TATTCATGGC AGTGTTGCCC CATCTCAGGT ACCGCTTTGC
    AGCAGCAGTT CTGTTCCCAG CTTTGCAGAG AGCGTGGCAG TCAGCCCCCT GCTGGATAGT
    GGCCAAAAGG CAGCAGAAGT GGGCACGGTA ATGAGCACGA CTGGCTTGTC TCTGCCCATC
    TCTGCACCTC TGACATATGC TGGGGTGGTT GCAGCATCAG GCATCACAAG TCAACCTGCC
    GTGCATCTCC CCCAGGGGGT GGCCACAACA CCAGTTGTAT CCCAGGCAGT AGGGGGAGCT
    GTGGGCACAC CCCTCTCATC TCTTCCTCCC ATCCCTGGAG GCATGTCTGT TAGCCAACCA
    GGATCAAATA TACCCCCTGT GCAACACACG TTGGCCCATG GCCAACCCCA GCCTACCCCT
    CTCCCCAACC AGCCTCATAC GCACCTTGTA GAGGGTGACA CCGATAACCT GCCCAAACCT
    GGCATAGACG ATATAAAAAC CCTGGAGGAG AAGCTGAGGT CGCTGTTCAG TGAGCATGGC
    AGTGCCGGGG CCCCTCATGG ACTCACCCCA TCAGAAACTC AATTGGGTTT GGATGGGGCC
    CCCCTGCCAG GCCCTTCCAC AGGTAGCACC GCTCAGACCA AGCCTGCAGC ATCCTCTTCT
    CCACCAACCA GCTTACCTTT AGGGCCACCT GGAGTGACGA CAATGACTCA GATCATGACA
    CCTGGACAAG CTGCTACACC TGTCACCTAC GTATCTGCAG CTGGAAGCTC TATAGCCACG
    GCCAAAGCTG CCACCCCACC CTCAAAGCCG CCTCTCAGTC GGGTACCGGT GACCACGGCC
    GGGGCAGAGA CCCCAGCAGG CAGTCTCCCT GGTGAACACC TGCCTCCTCC TTCTCTCCCA
    GGACCTTCAC TAACTCAGTC CCAGCAGCCT CTAGAAGACC TGGATTTCCA GCTGAGAAGG
    ACGTTGAGCC CTGACACTGT CCCTGTGCCT TCTGCACTAA TATTTCCTGC ACACAGCGGA
    GCTTCTACCG GTGTTGCAGC ACCCGTCGGC TCAGCATTGC AACCCTCCGC TTCAGAATTG
    CCAAAAGCCA GCGCTGGAGA GGCAGAGGTG GGCGCGAGTG TCCAGAGGAT GGGCAGGTTT
    CAGGTCTCTT TGGCAGCAGA GAATGTGCTC AAGCAGAGCG AGGAGACCAA ACTGTCCATC
    TCAGACACTT CCTCCACCAC TTCTACATCC TCCTCTTCCT CTGCGTCATG CAGCAGCCCA
    GAGAGCACCC TGACTAAGCC CCAGGCCAAA ACCGCTGCTC CCTTAGACAT AGTGGATGGT
    GTTGGTCCCC ACTTGGTGCA TCTACAGAGC CTATCTCCCA AGCAGTTGGT GAAAGTGGGG
    CGCTTCCAGG TTACCACTGC CACCACGGAC AAAGTGGGGC GCTTTTCTGT GTCCCGGACA
    CAAGATGATA TATCTGCTGG GGTGCTGGAG ACAGACTCCC AGAGGTCCTC CTCCCCACAG
    ACTGAGAAAC ATCTGAATGG ACCCTACTTA CAGCAAGACT CCTCTGCGGC TCTCGGCTAT
    CCGGCCAGGG AGACGCAGGA AGACAGCCTA GGGAGCCCCG TTCTGCACCA CGCTTTGGGG
    TCCAAGCTTA GCCTGCAGAG CCTTAGCAAC TCGTTCAACT CTTCCTACAT GAGCAGCGAT
    AATGATTCTG ACATAGAAGA TGAAGATCTG AAGAAGGAGC TGCGAAGGCT ACGAGACAAG
    CATCTCAAGG AGATTCAGGA ACTGCAGAGT CGACAGAAGC AGGAGATCGA GCTGTTGTAC
    ACGGCTTTGG GAAAAGTGCC CCCTGCTGTC ATTATCCCTC CGGCAGCGCC CCTTTCTGGC
    CGGCGCAGGA GGCATACCAA GGGGAAGGGC AGCAAGTCCA GCCGGGGCAG CTCCCACAAC
    AGCCGCAGCC CTCATTTGTC AGGCGACCTG TCCACGCAGA GCGCTCCGTC GGCATTACCC
    CCCCAGCCGG GCCTTCTGCT CCCCCCAAGC AGTGCGCCCG AACATGCTCA TCTTCTCAAG
    CCCTCCCCTT CCAGCGACAT TCTCTGCTCA GCCTTCACTA GCGACGCTGC CCTCTCTGTG
    CCCAGCCTGT CCACCCAGGG CCAAGGGACC AGCAGCACTA ACACAGTCGC ACCATCAACT
    GCCCCGGCCC CCCAGCCAAA CGTGCCCCCC GTGTCATCCA GCCGCAAGGG AACCTTCACG
    GACGATCTAC ACAAACTGGT GGACAACTGG GCCCGAGACG CCATGAACCT GACTGGAAAA
    AAGGCTTGTA AGGCCTCTGG GCAACCCAGC TATGAGGGAC CCGGGATGGC CCGTAAGTTC
    TCTGCTCCCG GACAGCTGTG TGTCCCCATG AACCCATCCC TGGGGGGCTC CGTCCCTCTC
    CCAGCAGCTT CGGCGACCTC CCTGGCTCAC TACCCCAAAG GAATGTGCCC CCCGCAACAA
    TACGGAATCC CCGGGGCGCT GTTCCAAGCT CCATGGAGTG GCACCGGCGT GCCCCCCTCC
    TCTCAGCCCC TAAGCCAGTT CCCCCCGGCG GTGACATCTT CCTTACAGAA CTTCGGTATT
    AGCAGCTTGC AGAAATCTAT CAGCAACCCC CAAGGATCCA ACCTGCGGAC TACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54273
    Clone ID Related Accession (Same CDS sequence) XM_031898633.1
    Accession Version XM_031898633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCATCGTCC ATGGTCCATT CAGCACTCCC AGACTCTCCG
    CTTTTTTTCC CAACCATTCC CAACCAGCGC CCCATGTCTT TCTCTCCGCC GCCTACTGGC
    TTGTCCCCAA AGGTGCCTGC AACCCAGCGA CGCAAGAGCA CCTCTTTCTT GGAGTCCCAA
    GGCCGCCAGG GGCTATCTCA GATTAGGACA CTCGGGCCAG GTGAGGAGAC CCAGTGCTGG
    AGGGCAGACT ACATGGCAGC TCTAGGTAAA GCATCTGTCC GATCCGAGGA TGCTCACAAG
    CATCAAAACT ATGAGGGATT GGCGTTCCAT GACGCAAGAC ATGTTCCGAC AGTTCCAAAT
    GAAACTCTCT TAAAGCCTGG GGAAGCATTG TATCTAGCCA GTCTGCCCTC ACAGGAGAGC
    TACAGCAGCA TGGCTGCCCC TGAGCATATG GAGGGCCACA AGCTGACCCA GCTTGGCTCG
    TTTTACGATT ATCACACTGG GCAAACCTAC ATTGCGCGGC CCCTGAAGAG CCTAAGACTG
    GAATCTGGGT TAAACCAGCC GTCGGCTTTG GGTAGCATCT CCAACCCAAT CTCCTCCGAC
    CCACACGCCA TGTTTCATCA GATGTTTGTG CCCCAGTCTG CTCCTCCAGT GCTGGCTCAA
    AGCAGCGAAG GCCACCCAGG GTGTGTGTTT GAGTTCCACG TGCACCCATC TGGGTCTGTT
    CCCGGCGAAG CAACCGTTTA CTTGCCACAC CGAGTTTACC GTCCTCGACG GGGCTCTGTA
    GACTTTAACC CAGAAGAACA GCCGCCGGGT TCTACGCTGC AGAGCCGCCT CCAAACTGTG
    ACTGAAGAGC AGTGCCACCA CAATGGCCCA GAACCACTCC CACCCTTTAT AGGATTCATC
    ACATGCAACC GTATGGAGAG CAGCCCCGAC TATTCCAGTG ATTCTTCCCA AAGGAATTCA
    TCCGATCCCG ATTATCTCTC TCCTCCTCTG GCCTTCAGCT CCAATATCTC TATGGCTTCC
    AAGCAGAAAA CGTTGCAGGA TCCACAGATT TTCTTTTGTG TCCCACAGTC CGGAACGACG
    GCTTATGCTG GACCATCAGC TTTGTACAGC CAGCAGCCAC AAATATCATC TGGACAACCA
    CCCACCACAT CCATGGGAAT CAGTGCTCAG CCCGGTCCCC ACACCCAGGG CATGGTGCAT
    CATCAGCCTT CTCTGCATCC AGCCTCGCAG TACCAAGTGC AGCAGCCAAT GCCTACTGTG
    GGTTCCAGTA TTGCGGCCCC ATCACAGCCG GTGATTACCC AAACGCCAGC CCCTCAGCCG
    GGCACCCAGC TCCCTGCCTC CCAGCCTTTG CCTGCTGTTC AAGGAGAGCC TCAGATTCCT
    GCTCAGACAG CTCCTGCTCC CCAGCCCGTT CCTACAGTGC CGGCCTCTCT GCCACCTCAT
    TTTCTTCCCT TGGCACAATC TTTGGTTGCA GTCCCTCTCA TTCCTCCATT CGCTGCCCCA
    CAGTTGCCTT TAACGGTACC ACCCCCGGTC ATACAGACAG GGTTCCCTTC CCTACCCATG
    ACTGTAACCC CAAGTATTGG CCAAACTGTA CCAGGAATAC CCCCATCCTT AGGAGCGCCA
    GTCCTGCCCC ATGGGCAGGC AGTGTTAGTT CCCACTCACA TCACTAATGT TGCTCTCCAG
    CCTTTGGCAC CAATCCCCAC ACCTCCTCTG TGCCCTGTAA CTCAGCCCAT TGGATTACCG
    GCCACCCTGG GAAAAGCTGC TGAGCCCTTG CTTCCTCCGG GAGATACTTC TTACCAGGTT
    TTCCAGGCTA CTCACCCTCT ATTCCCTGGA GATGTAAGCT TTGTTCCTCC CGTTGCCATC
    CCCTCAGCAA TGGTATCTCC CCCTATCCGT GGTGAAAGTT TAGCCCAACC TGGCTTCCAT
    ACTCAGCCTT ATCCTGAAAA CAGCAATTTG TATTCCCATG GAGGGCCCAC TCCCCAGCCC
    GCTGTTCTCA CTACCCAGCC GGTGCCTGCA GGAACCCAGA CGCAACCTGC CGTCGAGGGT
    TCACAGAGTA GCTCCCTGGC AATTTCCCAG GATACCCAGT CATCTCCTCA AGCAACACAA
    ACTGCCTCCT CCATGGACAG CGCTCATTCA GACGTGGCAT CAGGACTGAG TGACGGAAAC
    GAGGGCAGCA CAGGTGGGAG ACACGAAGGC CGAGCCACTA AACGACACTA CCGAAAATCT
    GTGAGAAGCC GCTCCAGGCA CGAGAAAACT GCACGCCCAA AGCTTCGAAT CCTTAATGTT
    TCCAACAAAG GGGATCGTGT TGTAGAGTGT CAGCTGGAGA CTCACAACCG CAAGATGGTC
    ACCTTCAAAT TCGACCTGGA TGGAGACAAT CCAGAGGAGA TTGCTACTAT AATGGTACAG
    AATGTGTTTA TTTTGGCTAC GGAGAGAGAA TCCTTTGTAG AGCAGGTAAA AGACATAATT
    GAGAAGGCCG ATGAAATGTT GAGCGAAGAT GTGAGCGTGG AGCCGGAAGG AGAAACGAGG
    GCAGAAAGTT TGCAAATCAA GGATGATGGG GATTACCCAG AATCTCAGAA GATGAATTCT
    GAATTTCGAA TGCCAGATCC TGTATATTCC CTACCACAGA CAGCAGGGCT TATTCCCAGC
    GTTGTCACAC AAGTCGTTCA TTCTGCCGGA AGACGATTTA TTGTCAGTCC GGTGCCTGAA
    AGCCGTCTAA AAGAACAGTC ATTCTTCAGT ACTTCATTGC AGGACCCTCA GCTACCAGTC
    TCTGTCGCGC ATGGTCCAGG GATGAATTTG TCTCATTCTG CCTCTTCTGT GAGCCTGCAG
    CAAGCCTTTG CAGAACTACG CCAGTCACAG ATGAGCGAGG GTCCTAGCAC AGCTCCTCCC
    ATATTTAACC AAACTGGACC CCCCTTTCCA CCATCCATGA CTAGTCTGGT GGCAACTCTG
    CCTTCTAATG CAATGCCTAT CCTCCAGGCA GCCCCAGTGA TCCCCAGTAG CCTGCCATCT
    TCTGTGCCTC AGCCCCTGGT GGAGGTGGTT GTACCTCAGG CTGCTGGGAT GGCTCCAACT
    AGCAATGTTC CAAGCACCTT GTCCCCTCCC CTAACTGCAG TATCCAACCA GCACGCAGAC
    ATGATACCGG GTGCTGCTGT AGCAGCTATC GGATCACTTC CTGTGACATC ACTTCCTCCT
    TTGCCCATTG TGGGTGGAGT TCCTGTAGGC ACTGCAACCC TGAGCTCACC CCTAACACCC
    CTAATCAGTT CTGTCAGTAC AATGGGGGGG ACGGTTCCCC CATCTGTCAC ATCTCCCCCA
    GTGCCACAGA TAGCGGGTAG CGTTGCCGGG AGCCTGCCTC CTGCATCAGC CTCCGCCCCT
    CTTATTCATG GCAGTGTTGC CCCATCTCAG GTACCGCTTT GCAGCAGCAG TTCTGTTCCC
    AGCTTTGCAG AGAGCGTGGC AGTCAGCCCC CTGCTGGATA GTGGCCAAAA GGCAGCAGAA
    GTGGGCACGG TAATGAGCAC GACTGGCTTG TCTCTGCCCA TCTCTGCACC TCTGACATAT
    GCTGGGGTGG TTGCAGCATC AGGCATCACA AGTCAACCTG CCGTGCATCT CCCCCAGGGG
    GTGGCCACAA CACCAGTTGT ATCCCAGGCA GTAGGGGGAG CTGTGGGCAC ACCCCTCTCA
    TCTCTTCCTC CCATCCCTGG AGGCATGTCT GTTAGCCAAC CAGGATCAAA TATACCCCCT
    GTGCAACACA CGTTGGCCCA TGGCCAACCC CAGCCTACCC CTCTCCCCAA CCAGCCTCAT
    ACGCACCTTG TAGAGGGTGA CACCGATAAC CTGCCCAAAC CTGGCATAGA CGATATAAAA
    ACCCTGGAGG AGAAGCTGAG GTCGCTGTTC AGTGAGCATG GCAGTGCCGG GGCCCCTCAT
    GGACTCACCC CATCAGAAAC TCAATTGGGT TTGGATGGGG CCCCCCTGCC AGGCCCTTCC
    ACAGGTAGCA CCGCTCAGAC CAAGCCTGCA GCATCCTCTT CTCCACCAAC CAGCTTACCT
    TTAGGGCCAC CTGGAGTGAC GACAATGACT CAGATCATGA CACCTGGACA AGCTGCTACA
    CCTGTCACCT ACGTATCTGC AGCTGGAAGC TCTATAGCCA CGGCCAAAGC TGCCACCCCA
    CCCTCAAAGC CGCCTCTCAG TCGGGTACCG GTGACCACGG CCGGGGCAGA GACCCCAGCA
    GGCAGTCTCC CTGGTGAACA CCTGCCTCCT CCTTCTCTCC CAGGACCTTC ACTAACTCAG
    TCCCAGCAGC CTCTAGAAGA CCTGGATTTC CAGCTGAGAA GGACGTTGAG CCCTGACACT
    GTCCCTGTGC CTTCTGCACT AATATTTCCT GCACACAGCG GAGCTTCTAC CGGTGTTGCA
    GCACCCGTCG GCTCAGCATT GCAACCCTCC GCTTCAGAAT TGCCAAAAGC CAGCGCTGGA
    GAGGCAGAGG TGGGCGCGAG TGTCCAGAGG ATGGGCAGGT TTCAGGTCTC TTTGGCAGCA
    GAGAATGTGC TCAAGCAGAG CGAGGAGACC AAACTGTCCA TCTCAGACAC TTCCTCCACC
    ACTTCTACAT CCTCCTCTTC CTCTGCGTCA TGCAGCAGCC CAGAGAGCAC CCTGACTAAG
    CCCCAGGCCA AAACCGCTGC TCCCTTAGAC ATAGTGGATG GTGTTGGTCC CCACTTGGTG
    CATCTACAGA GCCTATCTCC CAAGCAGTTG GTGAAAGTGG GGCGCTTCCA GGTTACCACT
    GCCACCACGG ACAAAGTGGG GCGCTTTTCT GTGTCCCGGA CACAAGATGA TATATCTGCT
    GGGGTGCTGG AGACAGACTC CCAGAGGTCC TCCTCCCCAC AGACTGAGAA ACATCTGAAT
    GGACCCTACT TACAGCAAGA CTCCTCTGCG GCTCTCGGCT ATCCGGCCAG GGAGACGCAG
    GAAGACAGCC TAGGGAGCCC CGTTCTGCAC CACGCTTTGG GGTCCAAGCT TAGCCTGCAG
    AGCCTTAGCA ACTCGTTCAA CTCTTCCTAC ATGAGCAGCG ATAATGATTC TGACATAGAA
    GATGAAGATC TGAAGAAGGA GCTGCGAAGG CTACGAGACA AGCATCTCAA GGAGATTCAG
    GAACTGCAGA GTCGACAGAA GCAGGAGATC GAGCTGTTGT ACACGGCTTT GGGAAAAGTG
    CCCCCTGCTG TCATTATCCC TCCGGCAGCG CCCCTTTCTG GCCGGCGCAG GAGGCATACC
    AAGGGGAAGG GCAGCAAGTC CAGCCGGGGC AGCTCCCACA ACAGCCGCAG CCCTCATTTG
    TCAGGCGACC TGTCCACGCA GAGCGCTCCG TCGGCATTAC CCCCCCAGCC GGGCCTTCTG
    CTCCCCCCAA GCAGTGCGCC CGAACATGCT CATCTTCTCA AGCCCTCCCC TTCCAGCGAC
    ATTCTCTGCT CAGCCTTCAC TAGCGACGCT GCCCTCTCTG TGCCCAGCCT GTCCACCCAG
    GGCCAAGGCT GGGCCAAGTT TAACTGTGAG TCAGAGCGGG TATCCTTCAA AATGGCTGGC
    AGGAGGACGC GATTTCTGAG GAAGATGGTG AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT
    AGGACCAGCA GCACTAACAC AGTCGCACCA TCAACTGCCC CGGCCCCCCA GCCAAACGTG
    CCCCCCGTGT CATCCAGCCG CAAGGGAACC TTCACGGACG ATCTACACAA ACTGGTGGAC
    AACTGGGCCC GAGACGCCAT GAACCTGACT GGAAAAAAGG CTTGTAAGGC CTCTGGGCAA
    CCCAGCTATG AGGGACCCGG GATGGCCCGT AAGTTCTCTG CTCCCGGACA GCTGTGTGTC
    CCCATGAACC CATCCCTGGG GGGCTCCGTC CCTCTCCCAG CAGCTTCGGC GACCTCCCTG
    GCTCACTACC CCAAAGGAAT GTGCCCCCCG CAACAATACG GAATCCCCGG GGCGCTGTTC
    CAAGCTCCAT GGAGTGGCAC CGGCGTGCCC CCCTCCTCTC AGCCCCTAAG CCAGTTCCCC
    CCGGCGGTGA CATCTTCCTT ACAGAACTTC GGTATTAGCA GCTTGCAGAA ATCTATCAGC
    AACCCCCAAG GATCCAACCT GCGGACTACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754493.1
    CDS327..8399
    Translation

    Target ORF information:

    RefSeq Version XM_031898633.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 1 (wnk1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGTCCTCCG TGCCCGGCAG CAGCCTGGCC CCCCCCCGGC CCACCAATGG CTCCAGCTCT 
    GACACGTCAG TCGGGGGCCG CTCGGTGCCC AGGGAGGAAG CAGAAGAGCG GCCTGAGGAG
    TTCCGCCGCC GCCGCCACAC TATGGATAAG GACAAGGCAG AGCATCGCTT TTTCCGTCGC
    AGCGTCATCT GCGATTCCAA TGCGACGGCC CTGGAGCTGC CTTCCAACCA GAGCATCTTG
    GGCTCCCCGC CCGAGTCAGA GACCGGCCTG CAGCTACTCG TCTGCGGGGA GAGCACTTTG
    GCGGTCCCGC CAGCCCTGCC AGCGAAGGAA GCCGTAAAGC AAGTCCGTCC TGACGGCGCT
    GAGCAGCCCC AGCCGAGCCC AGAGGCGCAA CAGCAGCCCC AGGCAGGATC CGCGGAGGAG
    GTGAAAGTCC CCAAGCCTGT AAGCGAGCAG CCGGAGGAGG AGAGCGGCAG GACCGAGCAG
    GAGAAACTCG CCGATGAGAA TGAGAAGCTG GAGACCACGG CTGTGGGCTT CTCCCCGGAT
    GGCAGGTTCC TCAAGTTCGA TATAGAGATC GGGAGAGGCT CTTTCAAAAC GGTTTATAAA
    GGGCTGGATA CGGAGACTAC TGTGGAGGTG GCCTGGTGTG AACTGCAGGA CCGCAAGCTT
    TCCAAGTCTG AGCGACAGAG ATTTAAAGAA GAAGCAGGCA TGCTGAAAGG GTTACAGCAC
    CCCAACATCG TCCGCTTCTA CGATTCCTGG GAGTCGACGC TAAAGGGAAA GAAGTGTATC
    GTTCTGGTGA CGGAGCTGAT GACCTCGGGC ACTCTGAAAA CGTATCTAAA GAGGTTCAAA
    GTCATGAAGC TGAAGGTTCT GAGAAGCTGG TGCAGGCAGA TTTTGAAAGG ACTACAGTTC
    CTACACACTC GCACTCCTCC CATCATCCAC CGGGATCTCA AATGCGACAA CATCTTTATC
    ACCGGCCCGA CGGGCTCAGT GAAAATCGGG GACCTGGGTC TGGCCACTCT GAAGAGAGCA
    TCGTTTGCCA AAAGTGTCAT AGGTACCCCT GAGTTCATGG CCCCAGAGAT GTATGAGGAG
    AAATACGATG AGTCCGTTGA CGTGTATGCG TTTGGGATGT GCATGCTTGA GATGGCCACT
    TCTGAATACC CTTACTCCGA ATGCCAAAAC GCTGCACAGA TCTACCGGCG AGTCACCAGT
    GGCGTGAAGC CTGCCAGCTT CGATAAAGTC GCAATTCCAG AGGTGAAAGA AATCATCGAA
    GGGTGCATTC GCCAAAACAA GGACGAAAGG TATGCCATTA AGGACCTGCT GAACCATGCC
    TTTTTCCAAG AGGAAACTGG GGTCCGCGTG GAACTGGCAG AGGAAGATGA CGGGGAAAAG
    ATTGCTATCA AGCTTTGGCT GCGTATTGAG GATATCAAAA AACTCAAGGG CAAATACAAG
    GACAATGAGG CAATTGAGTT CTCCTTTGAC CTGGAGCGAG ATGTTCCGGA TGATGTGGCA
    CAGGAAATGG TGGAATCTGG CTACGTCTGT GAAGGAGACC ACAAGACTAT GGCAAAGGCA
    ATCAAAGATA GGGTGTCCCT GATCAAACGC AAGCGGGAAC AGAGGCAGCA AGTGCGGGAA
    GAGCAGGAGA AGAGGAAACA GGAGGAGATT AGCCAGCAGC AGCAGCAGCA ACAAGCAGAG
    CTTCAGCCTC AGAGTCCTCC TCAAGGCACC GTCAAACCCT CCACTGGCAC CGTTACCTCT
    GGCACACAAG TAGAACCCGA GGAGCCGGAA GCTGATCAGC ATCTACAGTA CCAGCAGCCA
    AGTGTATCCG TCACATCCGA TGGGACAATT GACAGTGGTC AGGGATCGTC CATGTATTCT
    GAATCTTATG CGGGAAACCA GCCGACAGTC TCCTTTGGCT CTCAGCCCGA TCCAAGCCTC
    ACTCCCAGCA TGGTGCCTGG CTTCAGCACT CCAAATATCC AACCCCCTGC CCAGCATGGC
    GGATACCCAG CTCCTGTAGG GCCATCGTCC ATGGTCCATT CAGCACTCCC AGACTCTCCG
    CTTTTTTTCC CAACCATTCC CAACCAGCGC CCCATGTCTT TCTCTCCGCC GCCTACTGGC
    TTGTCCCCAA AGGTGCCTGC AACCCAGCGA CGCAAGAGCA CCTCTTTCTT GGAGTCCCAA
    GGCCGCCAGG GGCTATCTCA GATTAGGACA CTCGGGCCAG GTGAGGAGAC CCAGTGCTGG
    AGGGCAGACT ACATGGCAGC TCTAGGTAAA GCATCTGTCC GATCCGAGGA TGCTCACAAG
    CATCAAAACT ATGAGGGATT GGCGTTCCAT GACGCAAGAC ATGTTCCGAC AGTTCCAAAT
    GAAACTCTCT TAAAGCCTGG GGAAGCATTG TATCTAGCCA GTCTGCCCTC ACAGGAGAGC
    TACAGCAGCA TGGCTGCCCC TGAGCATATG GAGGGCCACA AGCTGACCCA GCTTGGCTCG
    TTTTACGATT ATCACACTGG GCAAACCTAC ATTGCGCGGC CCCTGAAGAG CCTAAGACTG
    GAATCTGGGT TAAACCAGCC GTCGGCTTTG GGTAGCATCT CCAACCCAAT CTCCTCCGAC
    CCACACGCCA TGTTTCATCA GATGTTTGTG CCCCAGTCTG CTCCTCCAGT GCTGGCTCAA
    AGCAGCGAAG GCCACCCAGG GTGTGTGTTT GAGTTCCACG TGCACCCATC TGGGTCTGTT
    CCCGGCGAAG CAACCGTTTA CTTGCCACAC CGAGTTTACC GTCCTCGACG GGGCTCTGTA
    GACTTTAACC CAGAAGAACA GCCGCCGGGT TCTACGCTGC AGAGCCGCCT CCAAACTGTG
    ACTGAAGAGC AGTGCCACCA CAATGGCCCA GAACCACTCC CACCCTTTAT AGGATTCATC
    ACATGCAACC GTATGGAGAG CAGCCCCGAC TATTCCAGTG ATTCTTCCCA AAGGAATTCA
    TCCGATCCCG ATTATCTCTC TCCTCCTCTG GCCTTCAGCT CCAATATCTC TATGGCTTCC
    AAGCAGAAAA CGTTGCAGGA TCCACAGATT TTCTTTTGTG TCCCACAGTC CGGAACGACG
    GCTTATGCTG GACCATCAGC TTTGTACAGC CAGCAGCCAC AAATATCATC TGGACAACCA
    CCCACCACAT CCATGGGAAT CAGTGCTCAG CCCGGTCCCC ACACCCAGGG CATGGTGCAT
    CATCAGCCTT CTCTGCATCC AGCCTCGCAG TACCAAGTGC AGCAGCCAAT GCCTACTGTG
    GGTTCCAGTA TTGCGGCCCC ATCACAGCCG GTGATTACCC AAACGCCAGC CCCTCAGCCG
    GGCACCCAGC TCCCTGCCTC CCAGCCTTTG CCTGCTGTTC AAGGAGAGCC TCAGATTCCT
    GCTCAGACAG CTCCTGCTCC CCAGCCCGTT CCTACAGTGC CGGCCTCTCT GCCACCTCAT
    TTTCTTCCCT TGGCACAATC TTTGGTTGCA GTCCCTCTCA TTCCTCCATT CGCTGCCCCA
    CAGTTGCCTT TAACGGTACC ACCCCCGGTC ATACAGACAG GGTTCCCTTC CCTACCCATG
    ACTGTAACCC CAAGTATTGG CCAAACTGTA CCAGGAATAC CCCCATCCTT AGGAGCGCCA
    GTCCTGCCCC ATGGGCAGGC AGTGTTAGTT CCCACTCACA TCACTAATGT TGCTCTCCAG
    CCTTTGGCAC CAATCCCCAC ACCTCCTCTG TGCCCTGTAA CTCAGCCCAT TGGATTACCG
    GCCACCCTGG GAAAAGCTGC TGAGCCCTTG CTTCCTCCGG GAGATACTTC TTACCAGGTT
    TTCCAGGCTA CTCACCCTCT ATTCCCTGGA GATGTAAGCT TTGTTCCTCC CGTTGCCATC
    CCCTCAGCAA TGGTATCTCC CCCTATCCGT GGTGAAAGTT TAGCCCAACC TGGCTTCCAT
    ACTCAGCCTT ATCCTGAAAA CAGCAATTTG TATTCCCATG GAGGGCCCAC TCCCCAGCCC
    GCTGTTCTCA CTACCCAGCC GGTGCCTGCA GGAACCCAGA CGCAACCTGC CGTCGAGGGT
    TCACAGAGTA GCTCCCTGGC AATTTCCCAG GATACCCAGT CATCTCCTCA AGCAACACAA
    ACTGCCTCCT CCATGGACAG CGCTCATTCA GACGTGGCAT CAGGACTGAG TGACGGAAAC
    GAGGGCAGCA CAGGTGGGAG ACACGAAGGC CGAGCCACTA AACGACACTA CCGAAAATCT
    GTGAGAAGCC GCTCCAGGCA CGAGAAAACT GCACGCCCAA AGCTTCGAAT CCTTAATGTT
    TCCAACAAAG GGGATCGTGT TGTAGAGTGT CAGCTGGAGA CTCACAACCG CAAGATGGTC
    ACCTTCAAAT TCGACCTGGA TGGAGACAAT CCAGAGGAGA TTGCTACTAT AATGGTACAG
    AATGTGTTTA TTTTGGCTAC GGAGAGAGAA TCCTTTGTAG AGCAGGTAAA AGACATAATT
    GAGAAGGCCG ATGAAATGTT GAGCGAAGAT GTGAGCGTGG AGCCGGAAGG AGAAACGAGG
    GCAGAAAGTT TGCAAATCAA GGATGATGGG GATTACCCAG AATCTCAGAA GATGAATTCT
    GAATTTCGAA TGCCAGATCC TGTATATTCC CTACCACAGA CAGCAGGGCT TATTCCCAGC
    GTTGTCACAC AAGTCGTTCA TTCTGCCGGA AGACGATTTA TTGTCAGTCC GGTGCCTGAA
    AGCCGTCTAA AAGAACAGTC ATTCTTCAGT ACTTCATTGC AGGACCCTCA GCTACCAGTC
    TCTGTCGCGC ATGGTCCAGG GATGAATTTG TCTCATTCTG CCTCTTCTGT GAGCCTGCAG
    CAAGCCTTTG CAGAACTACG CCAGTCACAG ATGAGCGAGG GTCCTAGCAC AGCTCCTCCC
    ATATTTAACC AAACTGGACC CCCCTTTCCA CCATCCATGA CTAGTCTGGT GGCAACTCTG
    CCTTCTAATG CAATGCCTAT CCTCCAGGCA GCCCCAGTGA TCCCCAGTAG CCTGCCATCT
    TCTGTGCCTC AGCCCCTGGT GGAGGTGGTT GTACCTCAGG CTGCTGGGAT GGCTCCAACT
    AGCAATGTTC CAAGCACCTT GTCCCCTCCC CTAACTGCAG TATCCAACCA GCACGCAGAC
    ATGATACCGG GTGCTGCTGT AGCAGCTATC GGATCACTTC CTGTGACATC ACTTCCTCCT
    TTGCCCATTG TGGGTGGAGT TCCTGTAGGC ACTGCAACCC TGAGCTCACC CCTAACACCC
    CTAATCAGTT CTGTCAGTAC AATGGGGGGG ACGGTTCCCC CATCTGTCAC ATCTCCCCCA
    GTGCCACAGA TAGCGGGTAG CGTTGCCGGG AGCCTGCCTC CTGCATCAGC CTCCGCCCCT
    CTTATTCATG GCAGTGTTGC CCCATCTCAG GTACCGCTTT GCAGCAGCAG TTCTGTTCCC
    AGCTTTGCAG AGAGCGTGGC AGTCAGCCCC CTGCTGGATA GTGGCCAAAA GGCAGCAGAA
    GTGGGCACGG TAATGAGCAC GACTGGCTTG TCTCTGCCCA TCTCTGCACC TCTGACATAT
    GCTGGGGTGG TTGCAGCATC AGGCATCACA AGTCAACCTG CCGTGCATCT CCCCCAGGGG
    GTGGCCACAA CACCAGTTGT ATCCCAGGCA GTAGGGGGAG CTGTGGGCAC ACCCCTCTCA
    TCTCTTCCTC CCATCCCTGG AGGCATGTCT GTTAGCCAAC CAGGATCAAA TATACCCCCT
    GTGCAACACA CGTTGGCCCA TGGCCAACCC CAGCCTACCC CTCTCCCCAA CCAGCCTCAT
    ACGCACCTTG TAGAGGGTGA CACCGATAAC CTGCCCAAAC CTGGCATAGA CGATATAAAA
    ACCCTGGAGG AGAAGCTGAG GTCGCTGTTC AGTGAGCATG GCAGTGCCGG GGCCCCTCAT
    GGACTCACCC CATCAGAAAC TCAATTGGGT TTGGATGGGG CCCCCCTGCC AGGCCCTTCC
    ACAGGTAGCA CCGCTCAGAC CAAGCCTGCA GCATCCTCTT CTCCACCAAC CAGCTTACCT
    TTAGGGCCAC CTGGAGTGAC GACAATGACT CAGATCATGA CACCTGGACA AGCTGCTACA
    CCTGTCACCT ACGTATCTGC AGCTGGAAGC TCTATAGCCA CGGCCAAAGC TGCCACCCCA
    CCCTCAAAGC CGCCTCTCAG TCGGGTACCG GTGACCACGG CCGGGGCAGA GACCCCAGCA
    GGCAGTCTCC CTGGTGAACA CCTGCCTCCT CCTTCTCTCC CAGGACCTTC ACTAACTCAG
    TCCCAGCAGC CTCTAGAAGA CCTGGATTTC CAGCTGAGAA GGACGTTGAG CCCTGACACT
    GTCCCTGTGC CTTCTGCACT AATATTTCCT GCACACAGCG GAGCTTCTAC CGGTGTTGCA
    GCACCCGTCG GCTCAGCATT GCAACCCTCC GCTTCAGAAT TGCCAAAAGC CAGCGCTGGA
    GAGGCAGAGG TGGGCGCGAG TGTCCAGAGG ATGGGCAGGT TTCAGGTCTC TTTGGCAGCA
    GAGAATGTGC TCAAGCAGAG CGAGGAGACC AAACTGTCCA TCTCAGACAC TTCCTCCACC
    ACTTCTACAT CCTCCTCTTC CTCTGCGTCA TGCAGCAGCC CAGAGAGCAC CCTGACTAAG
    CCCCAGGCCA AAACCGCTGC TCCCTTAGAC ATAGTGGATG GTGTTGGTCC CCACTTGGTG
    CATCTACAGA GCCTATCTCC CAAGCAGTTG GTGAAAGTGG GGCGCTTCCA GGTTACCACT
    GCCACCACGG ACAAAGTGGG GCGCTTTTCT GTGTCCCGGA CACAAGATGA TATATCTGCT
    GGGGTGCTGG AGACAGACTC CCAGAGGTCC TCCTCCCCAC AGACTGAGAA ACATCTGAAT
    GGACCCTACT TACAGCAAGA CTCCTCTGCG GCTCTCGGCT ATCCGGCCAG GGAGACGCAG
    GAAGACAGCC TAGGGAGCCC CGTTCTGCAC CACGCTTTGG GGTCCAAGCT TAGCCTGCAG
    AGCCTTAGCA ACTCGTTCAA CTCTTCCTAC ATGAGCAGCG ATAATGATTC TGACATAGAA
    GATGAAGATC TGAAGAAGGA GCTGCGAAGG CTACGAGACA AGCATCTCAA GGAGATTCAG
    GAACTGCAGA GTCGACAGAA GCAGGAGATC GAGCTGTTGT ACACGGCTTT GGGAAAAGTG
    CCCCCTGCTG TCATTATCCC TCCGGCAGCG CCCCTTTCTG GCCGGCGCAG GAGGCATACC
    AAGGGGAAGG GCAGCAAGTC CAGCCGGGGC AGCTCCCACA ACAGCCGCAG CCCTCATTTG
    TCAGGCGACC TGTCCACGCA GAGCGCTCCG TCGGCATTAC CCCCCCAGCC GGGCCTTCTG
    CTCCCCCCAA GCAGTGCGCC CGAACATGCT CATCTTCTCA AGCCCTCCCC TTCCAGCGAC
    ATTCTCTGCT CAGCCTTCAC TAGCGACGCT GCCCTCTCTG TGCCCAGCCT GTCCACCCAG
    GGCCAAGGCT GGGCCAAGTT TAACTGTGAG TCAGAGCGGG TATCCTTCAA AATGGCTGGC
    AGGAGGACGC GATTTCTGAG GAAGATGGTG AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT
    AGGACCAGCA GCACTAACAC AGTCGCACCA TCAACTGCCC CGGCCCCCCA GCCAAACGTG
    CCCCCCGTGT CATCCAGCCG CAAGGGAACC TTCACGGACG ATCTACACAA ACTGGTGGAC
    AACTGGGCCC GAGACGCCAT GAACCTGACT GGAAAAAAGG CTTGTAAGGC CTCTGGGCAA
    CCCAGCTATG AGGGACCCGG GATGGCCCGT AAGTTCTCTG CTCCCGGACA GCTGTGTGTC
    CCCATGAACC CATCCCTGGG GGGCTCCGTC CCTCTCCCAG CAGCTTCGGC GACCTCCCTG
    GCTCACTACC CCAAAGGAAT GTGCCCCCCG CAACAATACG GAATCCCCGG GGCGCTGTTC
    CAAGCTCCAT GGAGTGGCAC CGGCGTGCCC CCCTCCTCTC AGCCCCTAAG CCAGTTCCCC
    CCGGCGGTGA CATCTTCCTT ACAGAACTTC GGTATTAGCA GCTTGCAGAA ATCTATCAGC
    AACCCCCAAG GATCCAACCT GCGGACTACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.