sall1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	sall1
Entrez Gene ID	100036618
Full Name	spalt-like transcription factor 1
Synonyms	hsal1,sal-1,sal1,sall1-a,sall1-b,sall1b,tbs,znf794
General protein information	Preferred Names spalt-like transcription factor 1 Names sal-like protein 1 sal-like 1 spalt-like 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012960358.2	XP_012815812.1	sal-like protein 1 isoform X1
XM_012960360.3	XP_012815814.2	sal-like protein 1 isoform X2
XM_031899695.1	XP_031755555.1	sal-like protein 1 isoform X2
XM_018092857.1	XP_017948346.1	sal-like protein 1 isoform X2
XM_012960360.2	XP_012815814.1	sal-like protein 1 isoform X3
NM_001097177.1	NP_001090646.1	sal-like protein 1
XM_012960358.3	XP_012815812.2	sal-like protein 1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Sall1	NP_067365.2
Danio rerio (zebrafish)	sall1a	NP_001139486.1
Canis lupus familiaris (dog)	SALL1	XP_005617623.1
Gallus gallus (chicken)	SALL1	NP_990038.1
Pan troglodytes (chimpanzee)	SALL1	XP_528651.4
Homo sapiens (human)	SALL1	NP_002959.2
	SALL1	XP_001088338.2
Xenopus tropicalis (tropical clawed frog)	sall1	NP_001090646.1
Bos taurus (cattle)	SALL1	NP_001192719.1
Rattus norvegicus (Norway rat)	Sall1	NP_001100885.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sall1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sall1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa00957	XM_012960358.3 Latest version!	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa43687	XM_018092857.1 Latest version!	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa55604	XM_012960360.3 Latest version!	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa55604	XM_031899695.1 Latest version!	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa00957	NM_001097177.1 Latest version!	Xenopus tropicalis spalt-like transcription factor 1 (sall1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa29819	XM_012960358.2 Latest version!	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa29820	XM_012960360.2 Latest version!	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sall1 ( XM_012960358.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815812.2 Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa00957
Clone ID Related Accession (Same CDS sequence)	NM_001097177.1 , XM_012960358.3
Accession Version	XM_012960358.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sal-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960358.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGA GGAAGCAGGC GAAACCCCAG CATTTCCAAT CGGACCCCGG CCTGGCCTTG 
CCTGGAAGTC ATGGTACACT GGATTTGCTA CAGCTAAGGA TCAGCAGAGA ATCTGAAGAA 
ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGACTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815812.2
CDS	1094..5086
Translation	MSRRKQAKPQHFQSDPGLALPGSHGTLDLLQLRISRESEEMKSEPEEIKLKFNHERNTERGQIHRTPKNKEAHVCSRCCAEFFELSDLLQHKKNCTKNQLVLIVNENPPPPAEASSPIPPSDNPDEHMNDTVNNSDQADCSDLSEHGEHGKPDKEESMEVEFSETNNSSSGSNKVDNGDSSSNNPTLGTSAITTSLPQVRDLTALGNFSMINSNVIIENLQSTRVAVAQFSQEAKTKGAANNKLAVPALMEQLLALQQQQIHQLHLIEQIRHQILLLASQSADMPTSASGPQGALRVSATPLTTLSSHLSQQLAAAAGLAQSLASQSASISGMKQLPPIQLPQSNPVNTVPSISSSSPNMNALASTVTSPSSDKVPSSTGGSQVSNPPAPVTSSPAFAISSLLSPASNPLLPQPAPNTTPFPGTLPSIGTTAEDLNSLRKSKPPSVTAFETKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSIPPEKPVTNWLDTKPVLPTLTTSVGMPLPPTIPTLTSFIKTEEPQPIAISHPTASPPDSVKSETATEPLLKKTSDLPDEAEAAMPILDKEEQQSQNSDCIQNLNTSACSPTTGSGISASFPNPLLPLMSEQFKAKFPFGGLLDVAPASETSKLQQLVENIDKKSSDPNECVICRRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPIAESYPDSMESDTGSFDEKTIDDLDNFSDENMEDCPDSSVPDTPKSVDASQDSLSSSPLPPEVSSITALENQMKLINAGLAEQLQASLKSTENGSIEGDGMTNDSSSLGGDMESQSAGSPAASESTYSMHALSPSNSTNDYLKSPNTDEKIHRALSLDPTSGLSPTPANGGALDLTSSNTDKVIKDEHLGVLFPFRERGKYKNTICDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPSSSMTPTPTVPSTPTNPLATIIKTEFNGFMHGSSQDIKEQPTNIVSSGSLPSSATSPVLLPALARRTPKQHYCNACGKSFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVDGPMAFLGGNPVKFPEMFQKDLGTRAGNADPSSFWNQYAAALSNGLAMKTNEISVIQNGGITPMAGSLGNGGSSPISGLTGSLEKLQNSEPNAPLAGLEKLASSENGTSFRFMRFVEDSKEIATN

Target ORF information:

RefSeq Version	XM_012960358.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960358.3

ORF Insert Sequence:

ATGTCTCGGA GGAAGCAGGC GAAACCCCAG CATTTCCAAT CGGACCCCGG CCTGGCCTTG 
CCTGGAAGTC ATGGTACACT GGATTTGCTA CAGCTAAGGA TCAGCAGAGA ATCTGAAGAA 
ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGACTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sall1 ( XM_018092857.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948346.1 Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa43687
Clone ID Related Accession (Same CDS sequence)	XM_018092857.1
Accession Version	XM_018092857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sal-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGA GGAAGCAGGC GAAACCCCAG CATTTCCAAT CGGACCCCGG CCTGGCCTTG 
CCTGGAAGTC ATGGAAACAC AGAGAGGGGT CAAATCCATC GGACGCCCAA GAACAAGGAG 
GCGCATGTCT GTAGCAGATG TTGTGCAGAG TTCTTTGAAC TATCAGATTT GTTGCAGCAC 
AAGAAGAACT GCACTAAAAA TCAATTGGTT CTAATTGTGA ATGAAAATCC ACCACCTCCC 
GCTGAAGCCA GCTCTCCTAT TCCCCCCTCG GATAATCCTG ATGAGCACAT GAATGACACA 
GTTAATAACT CAGATCAAGC AGACTGCAGT GACCTTTCAG AGCATGGTGA GCACGGCAAG 
CCTGACAAAG AAGAATCCAT GGAGGTGGAG TTTTCGGAAA CTAACAATAG CAGCAGTGGT 
TCTAACAAAG TGGACAATGG TGATTCAAGC AGTAATAATC CCACATTGGG TACCTCAGCT 
ATCACAACTT CGCTACCTCA AGTAAGGGAT CTGACAGCCT TGGGCAACTT CTCTATGATC 
AACAGCAATG TCATCATTGA GAACCTTCAG AGCACTAGAG TGGCAGTGGC ACAATTCTCC 
CAAGAAGCCA AAACTAAGGG GGCAGCAAAC AATAAGCTGG CAGTGCCTGC ACTCATGGAA 
CAGCTCTTGG CTTTACAGCA ACAACAGATT CACCAGTTGC ATCTGATTGA ACAAATCCGT 
CACCAAATAT TACTGTTGGC ATCACAGAGT GCAGACATGC CCACTTCAGC TAGTGGCCCT 
CAAGGGGCTT TAAGAGTATC TGCCACTCCA CTGAGTACTC TGAGTTCCCA TTTGTCCCAG 
CAGCTGGCTG CAGCAGCTGG CTTAGCACAA AGTCTTGCTA GCCAATCCGC GAGCATCAGT 
GGTATGAAGC AACTACCACC CATACAGCTA CCTCAGAGCA ATCCTGTTAA CACAGTTCCA 
TCCATCAGTA GTTCCTCTCC AAATATGAAT GCATTGGCAT CCACAGTTAC TTCACCCTCC 
TCAGACAAAG TACCTTCAAG CACCGGTGGC TCACAGGTCA GTAACCCACC AGCACCTGTA 
ACGTCATCAC CAGCTTTTGC AATAAGTAGT TTATTAAGTC CTGCATCCAA TCCACTACTA 
CCTCAGCCTG CCCCTAACAC CACACCTTTC CCTGGCACAT TGCCCAGCAT AGGAACAACA 
GCAGAGGATT TAAACTCACT GAGGAAAAGT AAGCCACCCA GCGTGACTGC TTTTGAAACT 
AAGAGTACAT CTGATGAGGC ATTCTTTAAA CACAAGTGTA GATTCTGTGC TAAGGTTTTT 
GGCAGTGACA GTGCCTTGCA GATCCATCTA CGTTCTCACA CCGGCGAGAG ACCATTTAAA 
TGCAACATTT GTGGAAACAG GTTTTCCACC AAAGGGAATC TTAAAGTTCA TTTTCAGCGT 
CACAAAGAGA AATATCCCCA TATTCAGATG AATCCATATC CAGTGCCGGA ACATTTAGAC 
AATATTCCTA CAAGCACCGG TATTCCATAT GGGATGTCTA TCCCACCAGA AAAACCTGTA 
ACAAACTGGC TGGACACTAA ACCAGTTTTA CCTACCTTAA CAACATCAGT GGGTATGCCT 
CTCCCACCAA CAATTCCCAC CTTGACTTCA TTTATAAAAA CTGAAGAGCC ACAACCTATA 
GCTATTAGTC ATCCCACTGC CAGCCCACCT GATTCAGTAA AAAGTGAGAC CGCTACAGAG 
CCACTTTTAA AAAAAACTAG TGATCTTCCA GATGAGGCTG AGGCTGCGAT GCCCATTCTA 
GACAAGGAGG AACAGCAGTC TCAAAATTCA GATTGCATCC AAAATCTTAA TACTTCTGCC 
TGCTCTCCCA CAACTGGCTC TGGCATCTCG GCCTCATTTC CAAATCCACT TTTGCCTCTA 
ATGTCAGAGC AATTTAAAGC AAAATTTCCA TTTGGAGGAC TTTTGGACGT GGCACCTGCA 
TCAGAGACTT CAAAACTTCA ACAGCTTGTA GAGAATATTG ACAAAAAGTC AAGTGACCCA 
AATGAGTGTG TTATTTGTCG CAGAGTCCTG AGTTGCCAGA GTGCTCTAAA AATGCATTAT 
AGGACACATA CTGGAGAACG ACCATTTAAA TGCAAAATTT GTGGTCGTGC TTTTACAACT 
AAAGGTAACT TAAAGACTCA TTACAGTGTC CATCGTGCCA TGCCACCTCT TAGAGTTCAG 
CATTCATGCC CAATTTGTCA AAAGAAATTT ACAAATGCTG TTGTACTTCA ACAGCATATT 
AGGATGCATA TGGGAGGGCA GATTCCTAAT ACTCCTATTG CTGAAAGCTA TCCTGACTCC 
ATGGAATCAG ATACTGGGTC CTTTGATGAG AAAACTATTG ATGATCTAGA CAATTTCTCA 
GATGAAAACA TGGAAGATTG TCCAGACAGC AGTGTCCCAG ACACCCCTAA ATCTGTTGAT 
GCATCTCAAG ACAGCCTTTC TTCTTCCCCA CTGCCGCCTG AAGTTTCAAG TATCACTGCT 
TTAGAGAATC AAATGAAATT AATTAATGCT GGACTTGCTG AACAGCTCCA AGCCAGTCTA 
AAGTCAACTG AAAATGGGTC TATTGAGGGT GATGGTATGA CCAATGACTC CTCATCTCTT 
GGTGGTGACA TGGAAAGCCA AAGTGCTGGA AGCCCTGCTG CCTCTGAATC GACTTACTCC 
ATGCATGCTT TGTCTCCTTC CAATAGCACA AATGACTATC TGAAATCACC CAACACAGAT 
GAGAAGATTC ATCGAGCTCT GTCTCTTGAC CCAACCAGTG GACTGTCCCC TACACCTGCT 
AATGGAGGGG CTTTGGACTT GACATCTAGC AACACAGATA AAGTGATCAA AGATGAACAT 
TTAGGTGTGC TATTTCCTTT CCGAGAACGG GGTAAATATA AAAACACTAT ATGTGATATT 
TGTGGCAAAA CCTTTGCATG TCAGAGTGCC TTGGACATTC ATTATAGAAG CCATACCAAA 
GAGAGACCAT TTATTTGCAC AGTCTGCAAT CGTGGCTTTT CAACAAAGGG CAATTTGAAA 
CAGCATATGC TCACTCACCA GATGAGGGAT CTACCATCAC AACTCTTTGA ACCCAGTTCC 
AGTATGACCC CAACCCCAAC AGTTCCCTCA ACACCTACTA ACCCACTGGC AACTATAATA 
AAGACTGAAT TTAATGGTTT CATGCATGGC TCTTCTCAGG ACATTAAAGA ACAGCCTACA 
AATATAGTTT CATCAGGATC TCTGCCATCT TCTGCTACAT CGCCAGTTCT GCTTCCTGCT 
TTGGCCAGAA GGACTCCTAA GCAGCATTAC TGCAATGCAT GTGGGAAATC ATTCTCTTCT 
TCCAGTGCTT TACAAATCCA TGAGCGGACT CACACTGGTG AAAAACCATT TGCCTGCACT 
ATATGTGGAA GAGCATTTAC TACAAAAGGC AACCTTAAGG TTCATATGGG AACTCACATG 
TGGAACAGCA CACCTGCAAG AAGAGGAAGA AGGCTTTCAG TTGATGGACC TATGGCTTTT 
CTTGGAGGAA ACCCTGTTAA GTTCCCTGAA ATGTTCCAGA AGGATTTAGG TACAAGGGCT 
GGCAATGCGG ATCCTTCAAG TTTTTGGAAT CAGTATGCAG CAGCGCTGTC CAATGGATTG 
GCAATGAAGA CCAATGAGAT TTCGGTCATT CAGAATGGTG GCATTACTCC AATGGCTGGA 
AGCTTGGGAA ACGGTGGGAG CTCCCCCATC AGTGGCCTGA CTGGAAGCCT AGAAAAGCTC 
CAGAACTCTG AACCAAATGC ACCTCTAGCT GGTCTGGAGA AATTAGCAAG CAGTGAAAAT 
GGCACATCCT TCAGATTTAT GCGCTTTGTG GAAGACAGCA AAGAAATTGC CACAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948346.1
CDS	213..4112
Translation	MSRRKQAKPQHFQSDPGLALPGSHGNTERGQIHRTPKNKEAHVCSRCCAEFFELSDLLQHKKNCTKNQLVLIVNENPPPPAEASSPIPPSDNPDEHMNDTVNNSDQADCSDLSEHGEHGKPDKEESMEVEFSETNNSSSGSNKVDNGDSSSNNPTLGTSAITTSLPQVRDLTALGNFSMINSNVIIENLQSTRVAVAQFSQEAKTKGAANNKLAVPALMEQLLALQQQQIHQLHLIEQIRHQILLLASQSADMPTSASGPQGALRVSATPLSTLSSHLSQQLAAAAGLAQSLASQSASISGMKQLPPIQLPQSNPVNTVPSISSSSPNMNALASTVTSPSSDKVPSSTGGSQVSNPPAPVTSSPAFAISSLLSPASNPLLPQPAPNTTPFPGTLPSIGTTAEDLNSLRKSKPPSVTAFETKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSIPPEKPVTNWLDTKPVLPTLTTSVGMPLPPTIPTLTSFIKTEEPQPIAISHPTASPPDSVKSETATEPLLKKTSDLPDEAEAAMPILDKEEQQSQNSDCIQNLNTSACSPTTGSGISASFPNPLLPLMSEQFKAKFPFGGLLDVAPASETSKLQQLVENIDKKSSDPNECVICRRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPIAESYPDSMESDTGSFDEKTIDDLDNFSDENMEDCPDSSVPDTPKSVDASQDSLSSSPLPPEVSSITALENQMKLINAGLAEQLQASLKSTENGSIEGDGMTNDSSSLGGDMESQSAGSPAASESTYSMHALSPSNSTNDYLKSPNTDEKIHRALSLDPTSGLSPTPANGGALDLTSSNTDKVIKDEHLGVLFPFRERGKYKNTICDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPSSSMTPTPTVPSTPTNPLATIIKTEFNGFMHGSSQDIKEQPTNIVSSGSLPSSATSPVLLPALARRTPKQHYCNACGKSFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVDGPMAFLGGNPVKFPEMFQKDLGTRAGNADPSSFWNQYAAALSNGLAMKTNEISVIQNGGITPMAGSLGNGGSSPISGLTGSLEKLQNSEPNAPLAGLEKLASSENGTSFRFMRFVEDSKEIATN

Target ORF information:

RefSeq Version	XM_018092857.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092857.1

ORF Insert Sequence:

ATGTCTCGGA GGAAGCAGGC GAAACCCCAG CATTTCCAAT CGGACCCCGG CCTGGCCTTG 
CCTGGAAGTC ATGGAAACAC AGAGAGGGGT CAAATCCATC GGACGCCCAA GAACAAGGAG 
GCGCATGTCT GTAGCAGATG TTGTGCAGAG TTCTTTGAAC TATCAGATTT GTTGCAGCAC 
AAGAAGAACT GCACTAAAAA TCAATTGGTT CTAATTGTGA ATGAAAATCC ACCACCTCCC 
GCTGAAGCCA GCTCTCCTAT TCCCCCCTCG GATAATCCTG ATGAGCACAT GAATGACACA 
GTTAATAACT CAGATCAAGC AGACTGCAGT GACCTTTCAG AGCATGGTGA GCACGGCAAG 
CCTGACAAAG AAGAATCCAT GGAGGTGGAG TTTTCGGAAA CTAACAATAG CAGCAGTGGT 
TCTAACAAAG TGGACAATGG TGATTCAAGC AGTAATAATC CCACATTGGG TACCTCAGCT 
ATCACAACTT CGCTACCTCA AGTAAGGGAT CTGACAGCCT TGGGCAACTT CTCTATGATC 
AACAGCAATG TCATCATTGA GAACCTTCAG AGCACTAGAG TGGCAGTGGC ACAATTCTCC 
CAAGAAGCCA AAACTAAGGG GGCAGCAAAC AATAAGCTGG CAGTGCCTGC ACTCATGGAA 
CAGCTCTTGG CTTTACAGCA ACAACAGATT CACCAGTTGC ATCTGATTGA ACAAATCCGT 
CACCAAATAT TACTGTTGGC ATCACAGAGT GCAGACATGC CCACTTCAGC TAGTGGCCCT 
CAAGGGGCTT TAAGAGTATC TGCCACTCCA CTGAGTACTC TGAGTTCCCA TTTGTCCCAG 
CAGCTGGCTG CAGCAGCTGG CTTAGCACAA AGTCTTGCTA GCCAATCCGC GAGCATCAGT 
GGTATGAAGC AACTACCACC CATACAGCTA CCTCAGAGCA ATCCTGTTAA CACAGTTCCA 
TCCATCAGTA GTTCCTCTCC AAATATGAAT GCATTGGCAT CCACAGTTAC TTCACCCTCC 
TCAGACAAAG TACCTTCAAG CACCGGTGGC TCACAGGTCA GTAACCCACC AGCACCTGTA 
ACGTCATCAC CAGCTTTTGC AATAAGTAGT TTATTAAGTC CTGCATCCAA TCCACTACTA 
CCTCAGCCTG CCCCTAACAC CACACCTTTC CCTGGCACAT TGCCCAGCAT AGGAACAACA 
GCAGAGGATT TAAACTCACT GAGGAAAAGT AAGCCACCCA GCGTGACTGC TTTTGAAACT 
AAGAGTACAT CTGATGAGGC ATTCTTTAAA CACAAGTGTA GATTCTGTGC TAAGGTTTTT 
GGCAGTGACA GTGCCTTGCA GATCCATCTA CGTTCTCACA CCGGCGAGAG ACCATTTAAA 
TGCAACATTT GTGGAAACAG GTTTTCCACC AAAGGGAATC TTAAAGTTCA TTTTCAGCGT 
CACAAAGAGA AATATCCCCA TATTCAGATG AATCCATATC CAGTGCCGGA ACATTTAGAC 
AATATTCCTA CAAGCACCGG TATTCCATAT GGGATGTCTA TCCCACCAGA AAAACCTGTA 
ACAAACTGGC TGGACACTAA ACCAGTTTTA CCTACCTTAA CAACATCAGT GGGTATGCCT 
CTCCCACCAA CAATTCCCAC CTTGACTTCA TTTATAAAAA CTGAAGAGCC ACAACCTATA 
GCTATTAGTC ATCCCACTGC CAGCCCACCT GATTCAGTAA AAAGTGAGAC CGCTACAGAG 
CCACTTTTAA AAAAAACTAG TGATCTTCCA GATGAGGCTG AGGCTGCGAT GCCCATTCTA 
GACAAGGAGG AACAGCAGTC TCAAAATTCA GATTGCATCC AAAATCTTAA TACTTCTGCC 
TGCTCTCCCA CAACTGGCTC TGGCATCTCG GCCTCATTTC CAAATCCACT TTTGCCTCTA 
ATGTCAGAGC AATTTAAAGC AAAATTTCCA TTTGGAGGAC TTTTGGACGT GGCACCTGCA 
TCAGAGACTT CAAAACTTCA ACAGCTTGTA GAGAATATTG ACAAAAAGTC AAGTGACCCA 
AATGAGTGTG TTATTTGTCG CAGAGTCCTG AGTTGCCAGA GTGCTCTAAA AATGCATTAT 
AGGACACATA CTGGAGAACG ACCATTTAAA TGCAAAATTT GTGGTCGTGC TTTTACAACT 
AAAGGTAACT TAAAGACTCA TTACAGTGTC CATCGTGCCA TGCCACCTCT TAGAGTTCAG 
CATTCATGCC CAATTTGTCA AAAGAAATTT ACAAATGCTG TTGTACTTCA ACAGCATATT 
AGGATGCATA TGGGAGGGCA GATTCCTAAT ACTCCTATTG CTGAAAGCTA TCCTGACTCC 
ATGGAATCAG ATACTGGGTC CTTTGATGAG AAAACTATTG ATGATCTAGA CAATTTCTCA 
GATGAAAACA TGGAAGATTG TCCAGACAGC AGTGTCCCAG ACACCCCTAA ATCTGTTGAT 
GCATCTCAAG ACAGCCTTTC TTCTTCCCCA CTGCCGCCTG AAGTTTCAAG TATCACTGCT 
TTAGAGAATC AAATGAAATT AATTAATGCT GGACTTGCTG AACAGCTCCA AGCCAGTCTA 
AAGTCAACTG AAAATGGGTC TATTGAGGGT GATGGTATGA CCAATGACTC CTCATCTCTT 
GGTGGTGACA TGGAAAGCCA AAGTGCTGGA AGCCCTGCTG CCTCTGAATC GACTTACTCC 
ATGCATGCTT TGTCTCCTTC CAATAGCACA AATGACTATC TGAAATCACC CAACACAGAT 
GAGAAGATTC ATCGAGCTCT GTCTCTTGAC CCAACCAGTG GACTGTCCCC TACACCTGCT 
AATGGAGGGG CTTTGGACTT GACATCTAGC AACACAGATA AAGTGATCAA AGATGAACAT 
TTAGGTGTGC TATTTCCTTT CCGAGAACGG GGTAAATATA AAAACACTAT ATGTGATATT 
TGTGGCAAAA CCTTTGCATG TCAGAGTGCC TTGGACATTC ATTATAGAAG CCATACCAAA 
GAGAGACCAT TTATTTGCAC AGTCTGCAAT CGTGGCTTTT CAACAAAGGG CAATTTGAAA 
CAGCATATGC TCACTCACCA GATGAGGGAT CTACCATCAC AACTCTTTGA ACCCAGTTCC 
AGTATGACCC CAACCCCAAC AGTTCCCTCA ACACCTACTA ACCCACTGGC AACTATAATA 
AAGACTGAAT TTAATGGTTT CATGCATGGC TCTTCTCAGG ACATTAAAGA ACAGCCTACA 
AATATAGTTT CATCAGGATC TCTGCCATCT TCTGCTACAT CGCCAGTTCT GCTTCCTGCT 
TTGGCCAGAA GGACTCCTAA GCAGCATTAC TGCAATGCAT GTGGGAAATC ATTCTCTTCT 
TCCAGTGCTT TACAAATCCA TGAGCGGACT CACACTGGTG AAAAACCATT TGCCTGCACT 
ATATGTGGAA GAGCATTTAC TACAAAAGGC AACCTTAAGG TTCATATGGG AACTCACATG 
TGGAACAGCA CACCTGCAAG AAGAGGAAGA AGGCTTTCAG TTGATGGACC TATGGCTTTT 
CTTGGAGGAA ACCCTGTTAA GTTCCCTGAA ATGTTCCAGA AGGATTTAGG TACAAGGGCT 
GGCAATGCGG ATCCTTCAAG TTTTTGGAAT CAGTATGCAG CAGCGCTGTC CAATGGATTG 
GCAATGAAGA CCAATGAGAT TTCGGTCATT CAGAATGGTG GCATTACTCC AATGGCTGGA 
AGCTTGGGAA ACGGTGGGAG CTCCCCCATC AGTGGCCTGA CTGGAAGCCT AGAAAAGCTC 
CAGAACTCTG AACCAAATGC ACCTCTAGCT GGTCTGGAGA AATTAGCAAG CAGTGAAAAT 
GGCACATCCT TCAGATTTAT GCGCTTTGTG GAAGACAGCA AAGAAATTGC CACAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sall1 ( XM_012960360.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815814.2 Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X3, mRNA.

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CloneID	OXa55604
Clone ID Related Accession (Same CDS sequence)	XM_012960360.3 , XM_031899695.1
Accession Version	XM_012960360.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sal-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGACTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815814.2
CDS	1124..4996
Translation	MKSEPEEIKLKFNHERNTERGQIHRTPKNKEAHVCSRCCAEFFELSDLLQHKKNCTKNQLVLIVNENPPPPAEASSPIPPSDNPDEHMNDTVNNSDQADCSDLSEHGEHGKPDKEESMEVEFSETNNSSSGSNKVDNGDSSSNNPTLGTSAITTSLPQVRDLTALGNFSMINSNVIIENLQSTRVAVAQFSQEAKTKGAANNKLAVPALMEQLLALQQQQIHQLHLIEQIRHQILLLASQSADMPTSASGPQGALRVSATPLTTLSSHLSQQLAAAAGLAQSLASQSASISGMKQLPPIQLPQSNPVNTVPSISSSSPNMNALASTVTSPSSDKVPSSTGGSQVSNPPAPVTSSPAFAISSLLSPASNPLLPQPAPNTTPFPGTLPSIGTTAEDLNSLRKSKPPSVTAFETKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSIPPEKPVTNWLDTKPVLPTLTTSVGMPLPPTIPTLTSFIKTEEPQPIAISHPTASPPDSVKSETATEPLLKKTSDLPDEAEAAMPILDKEEQQSQNSDCIQNLNTSACSPTTGSGISASFPNPLLPLMSEQFKAKFPFGGLLDVAPASETSKLQQLVENIDKKSSDPNECVICRRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPIAESYPDSMESDTGSFDEKTIDDLDNFSDENMEDCPDSSVPDTPKSVDASQDSLSSSPLPPEVSSITALENQMKLINAGLAEQLQASLKSTENGSIEGDGMTNDSSSLGGDMESQSAGSPAASESTYSMHALSPSNSTNDYLKSPNTDEKIHRALSLDPTSGLSPTPANGGALDLTSSNTDKVIKDEHLGVLFPFRERGKYKNTICDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPSSSMTPTPTVPSTPTNPLATIIKTEFNGFMHGSSQDIKEQPTNIVSSGSLPSSATSPVLLPALARRTPKQHYCNACGKSFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVDGPMAFLGGNPVKFPEMFQKDLGTRAGNADPSSFWNQYAAALSNGLAMKTNEISVIQNGGITPMAGSLGNGGSSPISGLTGSLEKLQNSEPNAPLAGLEKLASSENGTSFRFMRFVEDSKEIATN

Target ORF information:

RefSeq Version	XM_012960360.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960360.3

ORF Insert Sequence:

ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGACTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sall1 ( XM_031899695.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755555.1 Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X2, mRNA.

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CloneID	OXa55604
Clone ID Related Accession (Same CDS sequence)	XM_012960360.3 , XM_031899695.1
Accession Version	XM_031899695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sal-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGACTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755555.1
CDS	201..4073
Translation	MKSEPEEIKLKFNHERNTERGQIHRTPKNKEAHVCSRCCAEFFELSDLLQHKKNCTKNQLVLIVNENPPPPAEASSPIPPSDNPDEHMNDTVNNSDQADCSDLSEHGEHGKPDKEESMEVEFSETNNSSSGSNKVDNGDSSSNNPTLGTSAITTSLPQVRDLTALGNFSMINSNVIIENLQSTRVAVAQFSQEAKTKGAANNKLAVPALMEQLLALQQQQIHQLHLIEQIRHQILLLASQSADMPTSASGPQGALRVSATPLTTLSSHLSQQLAAAAGLAQSLASQSASISGMKQLPPIQLPQSNPVNTVPSISSSSPNMNALASTVTSPSSDKVPSSTGGSQVSNPPAPVTSSPAFAISSLLSPASNPLLPQPAPNTTPFPGTLPSIGTTAEDLNSLRKSKPPSVTAFETKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSIPPEKPVTNWLDTKPVLPTLTTSVGMPLPPTIPTLTSFIKTEEPQPIAISHPTASPPDSVKSETATEPLLKKTSDLPDEAEAAMPILDKEEQQSQNSDCIQNLNTSACSPTTGSGISASFPNPLLPLMSEQFKAKFPFGGLLDVAPASETSKLQQLVENIDKKSSDPNECVICRRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPIAESYPDSMESDTGSFDEKTIDDLDNFSDENMEDCPDSSVPDTPKSVDASQDSLSSSPLPPEVSSITALENQMKLINAGLAEQLQASLKSTENGSIEGDGMTNDSSSLGGDMESQSAGSPAASESTYSMHALSPSNSTNDYLKSPNTDEKIHRALSLDPTSGLSPTPANGGALDLTSSNTDKVIKDEHLGVLFPFRERGKYKNTICDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPSSSMTPTPTVPSTPTNPLATIIKTEFNGFMHGSSQDIKEQPTNIVSSGSLPSSATSPVLLPALARRTPKQHYCNACGKSFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVDGPMAFLGGNPVKFPEMFQKDLGTRAGNADPSSFWNQYAAALSNGLAMKTNEISVIQNGGITPMAGSLGNGGSSPISGLTGSLEKLQNSEPNAPLAGLEKLASSENGTSFRFMRFVEDSKEIATN

Target ORF information:

RefSeq Version	XM_031899695.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899695.1

ORF Insert Sequence:

ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGACTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sall1 ( NM_001097177.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001090646.1 Xenopus tropicalis spalt-like transcription factor 1 (sall1), mRNA.

Price & Availability↑

CloneID	OXa00957
Clone ID Related Accession (Same CDS sequence)	NM_001097177.1 , XM_012960358.3
Accession Version	NM_001097177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sal-like protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC127274.1. ##Evidence-Data-START## Transcript exon combination :: BC127274.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCGGA GGAAGCAGGC GAAACCCCAG CATTTCCAAT CGGACCCCGG CCTGGCCTTG 
CCTGGAAGTC ATGGTACACT GGATTTGCTA CAGCTAAGGA TCAGCAGAGA ATCTGAAGAA 
ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGACTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001090646.1
CDS	112..4104
Translation	MSRRKQAKPQHFQSDPGLALPGSHGTLDLLQLRISRESEEMKSEPEEIKLKFNHERNTERGQIHRTPKNKEAHVCSRCCAEFFELSDLLQHKKNCTKNQLVLIVNENPPPPAEASSPIPPSDNPDEHMNDTVNNSDQADCSDLSEHGEHGKPDKEESMEVEFSETNNSSSGSNKVDNGDSSSNNPTLGTSAITTSLPQVRDLTALGNFSMINSNVIIENLQSTRVAVAQFSQEAKTKGAANNKLAVPALMEQLLALQQQQIHQLHLIEQIRHQILLLASQSADMPTSASGPQGALRVSATPLTTLSSHLSQQLAAAAGLAQSLASQSASISGMKQLPPIQLPQSNPVNTVPSISSSSPNMNALASTVTSPSSDKVPSSTGGSQVSNPPAPVTSSPAFAISSLLSPASNPLLPQPAPNTTPFPGTLPSIGTTAEDLNSLRKSKPPSVTAFETKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSIPPEKPVTNWLDTKPVLPTLTTSVGMPLPPTIPTLTSFIKTEEPQPIAISHPTASPPDSVKSETATEPLLKKTSDLPDEAEAAMPILDKEEQQSQNSDCIQNLNTSACSPTTGSGISASFPNPLLPLMSEQFKAKFPFGGLLDVAPASETSKLQQLVENIDKKSSDPNECVICRRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPIAESYPDSMESDTGSFDEKTIDDLDNFSDENMEDCPDSSVPDTPKSVDASQDSLSSSPLPPEVSSITALENQMKLINAGLAEQLQASLKSTENGSIEGDGMTNDSSSLGGDMESQSAGSPAASESTYSMHALSPSNSTNDYLKSPNTDEKIHRALSLDPTSGLSPTPANGGALDLTSSNTDKVIKDEHLGVLFPFRERGKYKNTICDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPSSSMTPTPTVPSTPTNPLATIIKTEFNGFMHGSSQDIKEQPTNIVSSGSLPSSATSPVLLPALARRTPKQHYCNACGKSFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVDGPMAFLGGNPVKFPEMFQKDLGTRAGNADPSSFWNQYAAALSNGLAMKTNEISVIQNGGITPMAGSLGNGGSSPISGLTGSLEKLQNSEPNAPLAGLEKLASSENGTSFRFMRFVEDSKEIATN

Target ORF information:

RefSeq Version	NM_001097177.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spalt-like transcription factor 1 (sall1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097177.1

ORF Insert Sequence:

ATGTCTCGGA GGAAGCAGGC GAAACCCCAG CATTTCCAAT CGGACCCCGG CCTGGCCTTG 
CCTGGAAGTC ATGGTACACT GGATTTGCTA CAGCTAAGGA TCAGCAGAGA ATCTGAAGAA 
ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGACTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sall1 ( XM_012960358.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815812.1 Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X1, mRNA.

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CloneID	OXa29819
Clone ID Related Accession (Same CDS sequence)	XM_012960358.2
Accession Version	XM_012960358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sal-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGA GGAAGCAGGC GAAACCCCAG CATTTCCAAT CGGACCCCGG CCTGGCCTTG 
CCTGGAAGTC ATGGTACACT GGATTTGCTA CAGCTAAGGA TCAGCAGAGA ATCTGAAGAA 
ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGAGTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815812.1
CDS	1095..5087
Translation	MSRRKQAKPQHFQSDPGLALPGSHGTLDLLQLRISRESEEMKSEPEEIKLKFNHERNTERGQIHRTPKNKEAHVCSRCCAEFFELSDLLQHKKNCTKNQLVLIVNENPPPPAEASSPIPPSDNPDEHMNDTVNNSDQADCSDLSEHGEHGKPDKEESMEVEFSETNNSSSGSNKVDNGDSSSNNPTLGTSAITTSLPQVRDLTALGNFSMINSNVIIENLQSTRVAVAQFSQEAKTKGAANNKLAVPALMEQLLALQQQQIHQLHLIEQIRHQILLLASQSADMPTSASGPQGALRVSATPLSTLSSHLSQQLAAAAGLAQSLASQSASISGMKQLPPIQLPQSNPVNTVPSISSSSPNMNALASTVTSPSSDKVPSSTGGSQVSNPPAPVTSSPAFAISSLLSPASNPLLPQPAPNTTPFPGTLPSIGTTAEDLNSLRKSKPPSVTAFETKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSIPPEKPVTNWLDTKPVLPTLTTSVGMPLPPTIPTLTSFIKTEEPQPIAISHPTASPPDSVKSETATEPLLKKTSDLPDEAEAAMPILDKEEQQSQNSDCIQNLNTSACSPTTGSGISASFPNPLLPLMSEQFKAKFPFGGLLDVAPASETSKLQQLVENIDKKSSDPNECVICRRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPIAESYPDSMESDTGSFDEKTIDDLDNFSDENMEDCPDSSVPDTPKSVDASQDSLSSSPLPPEVSSITALENQMKLINAGLAEQLQASLKSTENGSIEGDGMTNDSSSLGGDMESQSAGSPAASESTYSMHALSPSNSTNDYLKSPNTDEKIHRALSLDPTSGLSPTPANGGALDLTSSNTDKVIKDEHLGVLFPFRERGKYKNTICDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPSSSMTPTPTVPSTPTNPLATIIKTEFNGFMHGSSQDIKEQPTNIVSSGSLPSSATSPVLLPALARRTPKQHYCNACGKSFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVDGPMAFLGGNPVKFPEMFQKDLGTRAGNADPSSFWNQYAAALSNGLAMKTNEISVIQNGGITPMAGSLGNGGSSPISGLTGSLEKLQNSEPNAPLAGLEKLASSENGTSFRFMRFVEDSKEIATN

Target ORF information:

RefSeq Version	XM_012960358.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960358.2

ORF Insert Sequence:

ATGTCTCGGA GGAAGCAGGC GAAACCCCAG CATTTCCAAT CGGACCCCGG CCTGGCCTTG 
CCTGGAAGTC ATGGTACACT GGATTTGCTA CAGCTAAGGA TCAGCAGAGA ATCTGAAGAA 
ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGAGTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sall1 ( XM_012960360.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815814.1 Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X3, mRNA.

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CloneID	OXa29820
Clone ID Related Accession (Same CDS sequence)	XM_012960360.2
Accession Version	XM_012960360.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sal-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGAGTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815814.1
CDS	1125..4997
Translation	MKSEPEEIKLKFNHERNTERGQIHRTPKNKEAHVCSRCCAEFFELSDLLQHKKNCTKNQLVLIVNENPPPPAEASSPIPPSDNPDEHMNDTVNNSDQADCSDLSEHGEHGKPDKEESMEVEFSETNNSSSGSNKVDNGDSSSNNPTLGTSAITTSLPQVRDLTALGNFSMINSNVIIENLQSTRVAVAQFSQEAKTKGAANNKLAVPALMEQLLALQQQQIHQLHLIEQIRHQILLLASQSADMPTSASGPQGALRVSATPLSTLSSHLSQQLAAAAGLAQSLASQSASISGMKQLPPIQLPQSNPVNTVPSISSSSPNMNALASTVTSPSSDKVPSSTGGSQVSNPPAPVTSSPAFAISSLLSPASNPLLPQPAPNTTPFPGTLPSIGTTAEDLNSLRKSKPPSVTAFETKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSIPPEKPVTNWLDTKPVLPTLTTSVGMPLPPTIPTLTSFIKTEEPQPIAISHPTASPPDSVKSETATEPLLKKTSDLPDEAEAAMPILDKEEQQSQNSDCIQNLNTSACSPTTGSGISASFPNPLLPLMSEQFKAKFPFGGLLDVAPASETSKLQQLVENIDKKSSDPNECVICRRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPIAESYPDSMESDTGSFDEKTIDDLDNFSDENMEDCPDSSVPDTPKSVDASQDSLSSSPLPPEVSSITALENQMKLINAGLAEQLQASLKSTENGSIEGDGMTNDSSSLGGDMESQSAGSPAASESTYSMHALSPSNSTNDYLKSPNTDEKIHRALSLDPTSGLSPTPANGGALDLTSSNTDKVIKDEHLGVLFPFRERGKYKNTICDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPSSSMTPTPTVPSTPTNPLATIIKTEFNGFMHGSSQDIKEQPTNIVSSGSLPSSATSPVLLPALARRTPKQHYCNACGKSFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVDGPMAFLGGNPVKFPEMFQKDLGTRAGNADPSSFWNQYAAALSNGLAMKTNEISVIQNGGITPMAGSLGNGGSSPISGLTGSLEKLQNSEPNAPLAGLEKLASSENGTSFRFMRFVEDSKEIATN

Target ORF information:

RefSeq Version	XM_012960360.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spalt-like transcription factor 1 (sall1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960360.2

ORF Insert Sequence:

ATGAAATCTG AACCAGAAGA AATAAAACTA AAGTTTAATC ATGAAAGAAA CACAGAGAGG 
GGTCAAATCC ATCGGACGCC CAAGAACAAG GAGGCGCATG TCTGTAGCAG ATGTTGTGCA 
GAGTTCTTTG AACTATCAGA TTTGTTGCAG CACAAGAAGA ACTGCACTAA AAATCAATTG 
GTTCTAATTG TGAATGAAAA TCCACCACCT CCCGCTGAAG CCAGCTCTCC TATTCCCCCC 
TCGGATAATC CTGATGAGCA CATGAATGAC ACAGTTAATA ACTCAGATCA AGCAGACTGC 
AGTGACCTTT CAGAGCATGG TGAGCACGGC AAGCCTGACA AAGAAGAATC CATGGAGGTG 
GAGTTTTCGG AAACTAACAA TAGCAGCAGT GGTTCTAACA AAGTGGACAA TGGTGATTCA 
AGCAGTAATA ATCCCACATT GGGTACCTCA GCTATCACAA CTTCGCTACC TCAAGTAAGG 
GATCTGACAG CCTTGGGCAA CTTCTCTATG ATCAACAGCA ATGTCATCAT TGAGAACCTT 
CAGAGCACTA GAGTGGCAGT GGCACAATTC TCCCAAGAAG CCAAAACTAA GGGGGCAGCA 
AACAATAAGC TGGCAGTGCC TGCACTCATG GAACAGCTCT TGGCTTTACA GCAACAACAG 
ATTCACCAGT TGCATCTGAT TGAACAAATC CGTCACCAAA TATTACTGTT GGCATCACAG 
AGTGCAGACA TGCCCACTTC AGCTAGTGGC CCTCAAGGGG CTTTAAGAGT ATCTGCCACT 
CCACTGAGTA CTCTGAGTTC CCATTTGTCC CAGCAGCTGG CTGCAGCAGC TGGCTTAGCA 
CAAAGTCTTG CTAGCCAATC CGCGAGCATC AGTGGTATGA AGCAACTACC ACCCATACAG 
CTACCTCAGA GCAATCCTGT TAACACAGTT CCATCCATCA GTAGTTCCTC TCCAAATATG 
AATGCATTGG CATCCACAGT TACTTCACCC TCCTCAGACA AAGTACCTTC AAGCACCGGT 
GGCTCACAGG TCAGTAACCC ACCAGCACCT GTAACGTCAT CACCAGCTTT TGCAATAAGT 
AGTTTATTAA GTCCTGCATC CAATCCACTA CTACCTCAGC CTGCCCCTAA CACCACACCT 
TTCCCTGGCA CATTGCCCAG CATAGGAACA ACAGCAGAGG ATTTAAACTC ACTGAGGAAA 
AGTAAGCCAC CCAGCGTGAC TGCTTTTGAA ACTAAGAGTA CATCTGATGA GGCATTCTTT 
AAACACAAGT GTAGATTCTG TGCTAAGGTT TTTGGCAGTG ACAGTGCCTT GCAGATCCAT 
CTACGTTCTC ACACCGGCGA GAGACCATTT AAATGCAACA TTTGTGGAAA CAGGTTTTCC 
ACCAAAGGGA ATCTTAAAGT TCATTTTCAG CGTCACAAAG AGAAATATCC CCATATTCAG 
ATGAATCCAT ATCCAGTGCC GGAACATTTA GACAATATTC CTACAAGCAC CGGTATTCCA 
TATGGGATGT CTATCCCACC AGAAAAACCT GTAACAAACT GGCTGGACAC TAAACCAGTT 
TTACCTACCT TAACAACATC AGTGGGTATG CCTCTCCCAC CAACAATTCC CACCTTGACT 
TCATTTATAA AAACTGAAGA GCCACAACCT ATAGCTATTA GTCATCCCAC TGCCAGCCCA 
CCTGATTCAG TAAAAAGTGA GACCGCTACA GAGCCACTTT TAAAAAAAAC TAGTGATCTT 
CCAGATGAGG CTGAGGCTGC GATGCCCATT CTAGACAAGG AGGAACAGCA GTCTCAAAAT 
TCAGATTGCA TCCAAAATCT TAATACTTCT GCCTGCTCTC CCACAACTGG CTCTGGCATC 
TCGGCCTCAT TTCCAAATCC ACTTTTGCCT CTAATGTCAG AGCAATTTAA AGCAAAATTT 
CCATTTGGAG GACTTTTGGA CGTGGCACCT GCATCAGAGA CTTCAAAACT TCAACAGCTT 
GTAGAGAATA TTGACAAAAA GTCAAGTGAC CCAAATGAGT GTGTTATTTG TCGCAGAGTC 
CTGAGTTGCC AGAGTGCTCT AAAAATGCAT TATAGGACAC ATACTGGAGA ACGACCATTT 
AAATGCAAAA TTTGTGGTCG TGCTTTTACA ACTAAAGGTA ACTTAAAGAC TCATTACAGT 
GTCCATCGTG CCATGCCACC TCTTAGAGTT CAGCATTCAT GCCCAATTTG TCAAAAGAAA 
TTTACAAATG CTGTTGTACT TCAACAGCAT ATTAGGATGC ATATGGGAGG GCAGATTCCT 
AATACTCCTA TTGCTGAAAG CTATCCTGAC TCCATGGAAT CAGATACTGG GTCCTTTGAT 
GAGAAAACTA TTGATGATCT AGACAATTTC TCAGATGAAA ACATGGAAGA TTGTCCAGAC 
AGCAGTGTCC CAGACACCCC TAAATCTGTT GATGCATCTC AAGACAGCCT TTCTTCTTCC 
CCACTGCCGC CTGAAGTTTC AAGTATCACT GCTTTAGAGA ATCAAATGAA ATTAATTAAT 
GCTGGACTTG CTGAACAGCT CCAAGCCAGT CTAAAGTCAA CTGAAAATGG GTCTATTGAG 
GGTGATGGTA TGACCAATGA CTCCTCATCT CTTGGTGGTG ACATGGAAAG CCAAAGTGCT 
GGAAGCCCTG CTGCCTCTGA ATCGACTTAC TCCATGCATG CTTTGTCTCC TTCCAATAGC 
ACAAATGACT ATCTGAAATC ACCCAACACA GATGAGAAGA TTCATCGAGC TCTGTCTCTT 
GACCCAACCA GTGGACTGTC CCCTACACCT GCTAATGGAG GGGCTTTGGA CTTGACATCT 
AGCAACACAG ATAAAGTGAT CAAAGATGAA CATTTAGGTG TGCTATTTCC TTTCCGAGAA 
CGGGGTAAAT ATAAAAACAC TATATGTGAT ATTTGTGGCA AAACCTTTGC ATGTCAGAGT 
GCCTTGGACA TTCATTATAG AAGCCATACC AAAGAGAGAC CATTTATTTG CACAGTCTGC 
AATCGTGGCT TTTCAACAAA GGGCAATTTG AAACAGCATA TGCTCACTCA CCAGATGAGG 
GATCTACCAT CACAACTCTT TGAACCCAGT TCCAGTATGA CCCCAACCCC AACAGTTCCC 
TCAACACCTA CTAACCCACT GGCAACTATA ATAAAGACTG AATTTAATGG TTTCATGCAT 
GGCTCTTCTC AGGACATTAA AGAACAGCCT ACAAATATAG TTTCATCAGG ATCTCTGCCA 
TCTTCTGCTA CATCGCCAGT TCTGCTTCCT GCTTTGGCCA GAAGGACTCC TAAGCAGCAT 
TACTGCAATG CATGTGGGAA ATCATTCTCT TCTTCCAGTG CTTTACAAAT CCATGAGCGG 
ACTCACACTG GTGAAAAACC ATTTGCCTGC ACTATATGTG GAAGAGCATT TACTACAAAA 
GGCAACCTTA AGGTTCATAT GGGAACTCAC ATGTGGAACA GCACACCTGC AAGAAGAGGA 
AGAAGGCTTT CAGTTGATGG ACCTATGGCT TTTCTTGGAG GAAACCCTGT TAAGTTCCCT 
GAAATGTTCC AGAAGGATTT AGGTACAAGG GCTGGCAATG CGGATCCTTC AAGTTTTTGG 
AATCAGTATG CAGCAGCGCT GTCCAATGGA TTGGCAATGA AGACCAATGA GATTTCGGTC 
ATTCAGAATG GTGGCATTAC TCCAATGGCT GGAAGCTTGG GAAACGGTGG GAGCTCCCCC 
ATCAGTGGCC TGACTGGAAG CCTAGAAAAG CTCCAGAACT CTGAACCAAA TGCACCTCTA 
GCTGGTCTGG AGAAATTAGC AAGCAGTGAA AATGGCACAT CCTTCAGATT TATGCGCTTT 
GTGGAAGACA GCAAAGAAAT TGCCACAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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sall1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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