rnf31 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following rnf31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rnf31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa21953 NM_001097175.1
Latest version!
Xenopus tropicalis ring finger protein 31 (rnf31), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa41613 XM_018092915.1
Latest version!
Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OXa47344 XM_031899674.1
Latest version!
Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47344 XM_031899717.1
Latest version!
Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47345 XM_031899951.1
Latest version!
Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47346 XM_012962713.3
Latest version!
Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa29815 XM_012962713.2
Latest version!
Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29815 XM_012962335.2
Latest version!
Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29816 XM_018092848.1
Latest version!
Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa21953
    Clone ID Related Accession (Same CDS sequence) NM_001097175.1
    Accession Version NM_001097175.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase RNF31
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC127272.1. ##Evidence-Data-START## Transcript exon combination :: BC127272.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGGTG GACGATTTGT TCTGTCTCTT
    TATGGCTACT CTCAGTCACT TCCTGATGGT CTAAGTTTTC CAGATTCTGT GCAGGAACCA
    GATGTCTCCA CTGTTGCTGC TGTAACAGCT GATGTGATTA TTCTTCACCA TGAACTCAAC
    CTACTCATCT CGAACATGCA CCCACGGCAC GAGGAAGCAG CACAAGAACT ATTAGGTGGT
    GAACTGCCAC AGCAGTCTCT TGTTGCTGAT TACGTAAGTG CTCCTAAAGA TCCACAACCA
    GAAACCACTT CTGCTGGCTG TTTTCTTTGT GGTACTCTAG TTTGTTCTGT GTTTTGTTCC
    TCTTGTAATG AGATGCTCTG TGAGGAGTGT GATAAGAGAG CACACTCTCA TCCAGTCCGT
    GCCCAACATG TGCGTTTACC ATGTGGAAAT ACAGCAAAGC CCAGGTCATT GGATCAGGAC
    TCCCCTTCTA CAGTGTCATC TCGCCCTCCT TGGAGATGTT ACTCTTGCCA TTTTGAAAAC
    AAAGGGGGTT CTGTTCTGTG TGTGGTATGT GAGCGCCCCC GTGGCTGTAC CAGCAGCCTT
    TCAAACCTAT CACTTGGAAG TACTTTACAG AGAGACAGCT GGGAATGCCA GGCTTGTACT
    CTGCAAAATT CTGCATCTGC AGTTCTGTGT GCAGTGTGTG ATCGTCCCCG ATTGGCAAGC
    AAACCAGCCC AGGATGGTTA CGATTCTGTT GCTTTGCTTT TGAGAGATAT ACAGAGGGAT
    AACAAGAGTA TCACATGCCC TGTGAGTGAA AAAAATGGTC TATTTGCCAT GAAGAATCAT
    CAGGAGAGTA TGCATGAACC AGAAAGCATT GGTCCTCTGA TAGATCATAC AAGGCAAGAA
    GAGGAGATTG CTAAAAGTGA GGGCTGGCAG TGTTCCCATT GCACTTTCTT TAACACACGA
    AATGGACGTG TTTGTGAGAT TTGTGACAGG ACCAGTGAGG GGACAGCAGC AACTGAACAA
    CCCAAGGCAC TTGATATGGC ACATGTGCAG GAGGAGATCC GGCAGAAGCG ACTAAGAGAG
    GATGGAGCTA AAATAGTGGC ATTGATCCGG GAAGGAGAGA AGTATGGGGT TCTACCTGAA
    GAGGTGTGTA GCGCAATACG ATATTCTGGT ACAGAAGTGC CTCAGTGGTG GCTGCAGACT
    GAACTTCCCT ATGTATTAGA GCGTCTGCTG GATGCTGCTT CACAGAAAGC AGGAGATACA
    ATTGGTGCCC TAACAGTGGA AGAAGCTCGT GATGCTTGGG TGTCCAGTGG TGGAGACATG
    GATGCTGCTG TCATCTTATG TGTGACAGAG CGAAGAAGGA AGGTTGATGC ACTCTCCGGC
    CTAGGTTTTC CAGAAAGGCA TAAAGTGGTA GCTGCTCTTT ATGAAAGTGC TGGAGATGTT
    GGTAGAGCAC TAAGTATATT ACAGAGACCA CTTCTGGAGC CTTTTCTTAT ACGCATGTGG
    GAAGAACCAC AACCACCCCT TCGGCTAGAC TCGGCAGATA GACAGGCAGT TCTCCGTAGG
    TTACTTGCAG AACATTCCTT GCACAGTTGG GGTCGGGCAG AGCTAGCTTT GTCATTGCTT
    ATTGAAGGAG AAGGGCGCTA TGAACTCCAG GATGTTGTAG AGGCAGTGCG GGAATCGCAG
    GACAGAGACT TTATCAAAAG GATGTTAACA CAGGAGTGTG CAGTGTGTGG ATGGGAGCTC
    CCCCGTAACA AGATGCGTTC TCTAACCTCA TGTGAGTGTT GTATCTGTCC TGATTGCTTC
    CGGATGCATT TCACTGTAGC AGTGAAAGAG AAACATATTA GAGACATGAT ATGTCCAGCT
    TGTGAGGAGC CAGAAATAAG CGACGAGGGG GAGCTTCTTC ACTATTTCTC TACTCTGGAT
    ATCCTGTTAC GGGACACTTT GGAGGTTGAT GCATATGATT TGTTCCATAA AAAACTAACT
    GAAAGAACAC TTATGAAGGA CCCAAAGTTC CTGTGGTGTA CACATTGTTC CTTTGGATTC
    ATTTATGAAC GAGATCAGCT GGATGTAAAA TGCCCCCAGT GTCAGTGCAG TTTCTGCAGG
    AAATGCAAGA GGCCTTGGGA AGAACAGCAT CGGTCTTTAA ATTGTGATGA TTTTCAGAAC
    TGGAAAAGGG AAAATGATGC AGAATACCAA GCTCAAGGAT TGGCTGTATA CCTGCTAGAG
    AATGGGATCA CCTGTCCCCA TTGTAAATTT TCTTATGCTC TTGCACGAGG GGGATGCATG
    CACTTCATAT GTTCTCAGTG TCGCCACCAG TTCTGCAGTG GCTGCTATAA CACCTTTCAC
    CAAAGAAATA AATGTACAGT CCCGTCCTGT ACGGTAAGGA TGTCTCTCCA TGCTCACCAT
    CCTAGAGACT GTCTCTTTTA CCTCCGAGAT TGGGATGTCA CACGTCTGCA AAGACTACTC
    CAGATGTTAC TGGTACAGTT TAACACAGAT CCACCCGCTG GAACACAAAC TGCTCCAGGA
    GGTGGGTGTC GTGTGATGGA GCAAAAGGAG ATACCTGATG GCCTGAGAGA CGAAGCTTGT
    GGCAAAATAA CACCTCCAGG ATATGCTGGT TTGTGCGAGT CTCATTATAA AGAATATCTT
    GTTAGTCGTA TTAATGCCCA TTCTCTGGAT CCATGTGTTC TCTATGATCT GGCTGAGACT
    ATGAATGTGT GTCAGCGCTA CCTGCCTGTA TTGCCTCTAC AAGATCCCAC CGAGGATGAG
    GCCAGCTACA GGGAGCGTCT CCTGGGGGTG TTAATATCAG AGGTGCCACT GAGTATGAAC
    ATTCCACGGA AGCAGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001090644.1
    CDS109..3069
    Translation

    Target ORF information:

    RefSeq Version NM_001097175.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 31 (rnf31), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001097175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGGTG GACGATTTGT TCTGTCTCTT
    TATGGCTACT CTCAGTCACT TCCTGATGGT CTAAGTTTTC CAGATTCTGT GCAGGAACCA
    GATGTCTCCA CTGTTGCTGC TGTAACAGCT GATGTGATTA TTCTTCACCA TGAACTCAAC
    CTACTCATCT CGAACATGCA CCCACGGCAC GAGGAAGCAG CACAAGAACT ATTAGGTGGT
    GAACTGCCAC AGCAGTCTCT TGTTGCTGAT TACGTAAGTG CTCCTAAAGA TCCACAACCA
    GAAACCACTT CTGCTGGCTG TTTTCTTTGT GGTACTCTAG TTTGTTCTGT GTTTTGTTCC
    TCTTGTAATG AGATGCTCTG TGAGGAGTGT GATAAGAGAG CACACTCTCA TCCAGTCCGT
    GCCCAACATG TGCGTTTACC ATGTGGAAAT ACAGCAAAGC CCAGGTCATT GGATCAGGAC
    TCCCCTTCTA CAGTGTCATC TCGCCCTCCT TGGAGATGTT ACTCTTGCCA TTTTGAAAAC
    AAAGGGGGTT CTGTTCTGTG TGTGGTATGT GAGCGCCCCC GTGGCTGTAC CAGCAGCCTT
    TCAAACCTAT CACTTGGAAG TACTTTACAG AGAGACAGCT GGGAATGCCA GGCTTGTACT
    CTGCAAAATT CTGCATCTGC AGTTCTGTGT GCAGTGTGTG ATCGTCCCCG ATTGGCAAGC
    AAACCAGCCC AGGATGGTTA CGATTCTGTT GCTTTGCTTT TGAGAGATAT ACAGAGGGAT
    AACAAGAGTA TCACATGCCC TGTGAGTGAA AAAAATGGTC TATTTGCCAT GAAGAATCAT
    CAGGAGAGTA TGCATGAACC AGAAAGCATT GGTCCTCTGA TAGATCATAC AAGGCAAGAA
    GAGGAGATTG CTAAAAGTGA GGGCTGGCAG TGTTCCCATT GCACTTTCTT TAACACACGA
    AATGGACGTG TTTGTGAGAT TTGTGACAGG ACCAGTGAGG GGACAGCAGC AACTGAACAA
    CCCAAGGCAC TTGATATGGC ACATGTGCAG GAGGAGATCC GGCAGAAGCG ACTAAGAGAG
    GATGGAGCTA AAATAGTGGC ATTGATCCGG GAAGGAGAGA AGTATGGGGT TCTACCTGAA
    GAGGTGTGTA GCGCAATACG ATATTCTGGT ACAGAAGTGC CTCAGTGGTG GCTGCAGACT
    GAACTTCCCT ATGTATTAGA GCGTCTGCTG GATGCTGCTT CACAGAAAGC AGGAGATACA
    ATTGGTGCCC TAACAGTGGA AGAAGCTCGT GATGCTTGGG TGTCCAGTGG TGGAGACATG
    GATGCTGCTG TCATCTTATG TGTGACAGAG CGAAGAAGGA AGGTTGATGC ACTCTCCGGC
    CTAGGTTTTC CAGAAAGGCA TAAAGTGGTA GCTGCTCTTT ATGAAAGTGC TGGAGATGTT
    GGTAGAGCAC TAAGTATATT ACAGAGACCA CTTCTGGAGC CTTTTCTTAT ACGCATGTGG
    GAAGAACCAC AACCACCCCT TCGGCTAGAC TCGGCAGATA GACAGGCAGT TCTCCGTAGG
    TTACTTGCAG AACATTCCTT GCACAGTTGG GGTCGGGCAG AGCTAGCTTT GTCATTGCTT
    ATTGAAGGAG AAGGGCGCTA TGAACTCCAG GATGTTGTAG AGGCAGTGCG GGAATCGCAG
    GACAGAGACT TTATCAAAAG GATGTTAACA CAGGAGTGTG CAGTGTGTGG ATGGGAGCTC
    CCCCGTAACA AGATGCGTTC TCTAACCTCA TGTGAGTGTT GTATCTGTCC TGATTGCTTC
    CGGATGCATT TCACTGTAGC AGTGAAAGAG AAACATATTA GAGACATGAT ATGTCCAGCT
    TGTGAGGAGC CAGAAATAAG CGACGAGGGG GAGCTTCTTC ACTATTTCTC TACTCTGGAT
    ATCCTGTTAC GGGACACTTT GGAGGTTGAT GCATATGATT TGTTCCATAA AAAACTAACT
    GAAAGAACAC TTATGAAGGA CCCAAAGTTC CTGTGGTGTA CACATTGTTC CTTTGGATTC
    ATTTATGAAC GAGATCAGCT GGATGTAAAA TGCCCCCAGT GTCAGTGCAG TTTCTGCAGG
    AAATGCAAGA GGCCTTGGGA AGAACAGCAT CGGTCTTTAA ATTGTGATGA TTTTCAGAAC
    TGGAAAAGGG AAAATGATGC AGAATACCAA GCTCAAGGAT TGGCTGTATA CCTGCTAGAG
    AATGGGATCA CCTGTCCCCA TTGTAAATTT TCTTATGCTC TTGCACGAGG GGGATGCATG
    CACTTCATAT GTTCTCAGTG TCGCCACCAG TTCTGCAGTG GCTGCTATAA CACCTTTCAC
    CAAAGAAATA AATGTACAGT CCCGTCCTGT ACGGTAAGGA TGTCTCTCCA TGCTCACCAT
    CCTAGAGACT GTCTCTTTTA CCTCCGAGAT TGGGATGTCA CACGTCTGCA AAGACTACTC
    CAGATGTTAC TGGTACAGTT TAACACAGAT CCACCCGCTG GAACACAAAC TGCTCCAGGA
    GGTGGGTGTC GTGTGATGGA GCAAAAGGAG ATACCTGATG GCCTGAGAGA CGAAGCTTGT
    GGCAAAATAA CACCTCCAGG ATATGCTGGT TTGTGCGAGT CTCATTATAA AGAATATCTT
    GTTAGTCGTA TTAATGCCCA TTCTCTGGAT CCATGTGTTC TCTATGATCT GGCTGAGACT
    ATGAATGTGT GTCAGCGCTA CCTGCCTGTA TTGCCTCTAC AAGATCCCAC CGAGGATGAG
    GCCAGCTACA GGGAGCGTCT CCTGGGGGTG TTAATATCAG AGGTGCCACT GAGTATGAAC
    ATTCCACGGA AGCAGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41613
    Clone ID Related Accession (Same CDS sequence) XM_018092915.1
    Accession Version XM_018092915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase RNF31 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGGTG GACGATTTGT TCTGTCTCTT
    TATGGCTACT CTCAGTCACT TCCTGATGGT CTAAGTTTTC CAGATTCTGT GCAGGAACCA
    GATGTCTCCA CTGTTGCTGC TGTAACAGCT GATGTGATTA TTCTTCACCA TGAACTCAAC
    CTACTCATCT CGAACATGCA CCCACGGCAC GAGGAAGCAG CACAAGAACT ATTAGGTGGT
    GAACTGCCAC AGCAGTCTCT TGTTGCTGAT TACGTAAGTG CTCCTAAAGA TCTACAACCA
    GAAACCACTT CTGCTGGCTG TTTTCTTTGT GGTACTCTAG TTTGTTCTGT GTTTTGTTCC
    TCTTGTAATG AGATGCTCTG TGAGGAGTGT GATAAGAGAG CACACTCTCA TCCAGTCCGT
    GCCCAACATG TGCGTTTACC ATGTGGAAAT ACAGCAAAGC CCAGGTCATT GGATCAGGAC
    TCCCCTTCTA CAGTGTCATC TCGCCCTCCT TGGAGATGTT ACTCTTGCCA TTTTGAAAAC
    AAAGGGGGTT CTGTTCTGTG TGTGGTATGT GAGCGCCCCC GTGGCTGTAC CAGCAGCCTT
    TCAAACCTAT CACTTGGAAG TACTTTACAG AGAGACAGCT GGGAATGCCA GGCTTGTACT
    CTGCAAAATT CTGCATCTGC AGTTCTGTGT GCAGTGTGTG ATCGTCCCCG ATTGGCAAGC
    AAACCAGCCC AGGATGGTTA CGATTCTGTT GCTTTGCTTT TGAGAGATAT ACAGAGGGAT
    AACAAGAGTA TCACATGCCC TGTGAGTGAA AAAAATGGTC TATTTGCCAT GAAGAATCAT
    CAGGAGAGTA TGCATGAACC AGAAAGCATT GGTCCTCTGA TAGATCATAC AAGGCAAGAA
    GAGGAGATTG CTAAAAGTGA GGGCTGGCAG TGTTCCCATT GCACTTTCTT TAACACACGA
    AATGGACGTG TTTGTGAGAT TTGTGACAGG ACCAGTGAGG GGACAGCAGC AACTGAACAA
    CCCAAGGCAC TTGATATGGC ACATGTGCAG GAGGAGATCC GGCAGAAGCG ACTAAGAGAG
    GATGGAGCTA AAATAGTGGC ATTGATCCGG GAAGGAGAGA AGTATGGGGT TCTACCTGAA
    GAGGTGTGTA GCGCAATACG ATATTCTGGT ACAGAAGTGC CTCAGTGGTG GCTGCAGACT
    GAACTTCCCT ATGTATTAGA GCGTCTGCTG GATGCTGCTT CACAGAAAGC AGGAGATACA
    ATTGGTGCCC TAACAGTGGA AGAAGCTCGT GATGCTTGGG TGTCCAGTGG TGGAGACATG
    GATGCTGCTG TCATCTTATG TGTGACAGAG CGAAGAAGGA AGGTTGATGC ACTCTCCGGC
    CTAGGTTTTC CAGAAAGGCA TAAAGTGGTA GCTGCTCTTT ATGAAAGTGC TGGAGATGTT
    GGTAGAGCAC TAAGTATATT ACAGAGACCA CTTCTGGAGC CTTTTCTTAT ACGCATGTGG
    GAAGAACCAC AACCACCCCT TCGGCTAGAC TCAGCAGATA GACAGGCAGT TCTCCGTAGG
    TTACTTGCAG AACATTCCTT GCACAGTTGG GGTCGGGCAG AGCTAGCTTT GTCATTGCTT
    ATTGAAGGAG AAGGGCGCTA TGAACTCCAG GATGTTGTAG AGGCAGTGCG GGAATCGCAG
    GACAGAGACT TTATCAAAAG GATGTTAACA CAGGAGTGTG CAGTGTGTGG ATGGGAGCTC
    CCCCGTAACA AGATGCGTTC TCTAACCTCA TGTGAGTGTT GTATCTGTCC TGATTGCTTC
    CGGATGCATT TCACTGTAGC AGTGAAAGAG AAACATATTA GAGACATGAT ATGTCCAGCT
    TGTGAGGAGC CAGAAATAAG CGACGAGGGG GAGCTTCTTC ACTATTTCTC TACTCTGGAT
    ATCCTGTTAC GGGACACTTT GGAGGTTGAT GCATATGATT TGTTCCATAA AAAACTAACT
    GAAAGAACAC TTATGAAGGA CCCAAAGTTC CTGTGGTGTA CACATTGTTC CTTTGGATTC
    ATTTATGAAC GAGATCAGCT GGATGTAAAA TGCCCCCAGT GTCAGTGCAG TTTCTGCAGG
    AAATGCAAGA GGCCTTGGGA AGAACAGCAT CGGTCTTTAA ATTGTGATGA TTTTCAGAAC
    TGGAAAAGGG AAAATGATGC AGAATACCAA GCTCAAGGAT TGGCTGTATA CCTGCTAGAG
    AATGGGATCA CCTGTCCCCA TTGTAAATTT TCTTATGCTC TTGCACGAGG GGGATGCATG
    CACTTCATAT GTTCTCAGTG TCGCCACCAG TTCTGCAGTG GCTGCTATAA CACCTTTCAC
    CAAAGAAATA AATGTACAGT CCCGTCCTGT ACGGTAAGGA TGTCTCTCCA TGCTCACCAT
    CCTAGAGACT GTCTCTTTTA CCTCCGAGAT TGGGATGTCA CACGTCTGCA AAGACTACTC
    CAGATGTTAC TGGTACAGTT TAACACAGAT CCACCCGCTG GAACACAAAC TGCTCCAGGA
    GGTGGGTGTC GTGTGATGGA GCAAAAGGAG ATACCTGATG GCCTGAGAGA CGAAGCTTGT
    GGCAAAATAA CACCTCCAGG ATATGCTGGT TTGTGCGAGT CTCATTATAA AGAATATCTT
    GTTAGTCGTA TTAATGCCCA TTCTCTGGAT CCATGTGTTC TCTATGATCT GGCTGAGACT
    ATGAATGTGT GTCAGCGCTA CCTGCCTGTA TTGCCTCTAC AAGATCCCAC CGAGAATGAG
    GCCAGCTACA GGGAGCGTCT CCTGGGGGTG TTAATATCAG AGGTGCCACT GAGTATGAAC
    ATTCCACGGA AGCAGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948404.1
    CDS5..2965
    Translation

    Target ORF information:

    RefSeq Version XM_018092915.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGGTG GACGATTTGT TCTGTCTCTT
    TATGGCTACT CTCAGTCACT TCCTGATGGT CTAAGTTTTC CAGATTCTGT GCAGGAACCA
    GATGTCTCCA CTGTTGCTGC TGTAACAGCT GATGTGATTA TTCTTCACCA TGAACTCAAC
    CTACTCATCT CGAACATGCA CCCACGGCAC GAGGAAGCAG CACAAGAACT ATTAGGTGGT
    GAACTGCCAC AGCAGTCTCT TGTTGCTGAT TACGTAAGTG CTCCTAAAGA TCTACAACCA
    GAAACCACTT CTGCTGGCTG TTTTCTTTGT GGTACTCTAG TTTGTTCTGT GTTTTGTTCC
    TCTTGTAATG AGATGCTCTG TGAGGAGTGT GATAAGAGAG CACACTCTCA TCCAGTCCGT
    GCCCAACATG TGCGTTTACC ATGTGGAAAT ACAGCAAAGC CCAGGTCATT GGATCAGGAC
    TCCCCTTCTA CAGTGTCATC TCGCCCTCCT TGGAGATGTT ACTCTTGCCA TTTTGAAAAC
    AAAGGGGGTT CTGTTCTGTG TGTGGTATGT GAGCGCCCCC GTGGCTGTAC CAGCAGCCTT
    TCAAACCTAT CACTTGGAAG TACTTTACAG AGAGACAGCT GGGAATGCCA GGCTTGTACT
    CTGCAAAATT CTGCATCTGC AGTTCTGTGT GCAGTGTGTG ATCGTCCCCG ATTGGCAAGC
    AAACCAGCCC AGGATGGTTA CGATTCTGTT GCTTTGCTTT TGAGAGATAT ACAGAGGGAT
    AACAAGAGTA TCACATGCCC TGTGAGTGAA AAAAATGGTC TATTTGCCAT GAAGAATCAT
    CAGGAGAGTA TGCATGAACC AGAAAGCATT GGTCCTCTGA TAGATCATAC AAGGCAAGAA
    GAGGAGATTG CTAAAAGTGA GGGCTGGCAG TGTTCCCATT GCACTTTCTT TAACACACGA
    AATGGACGTG TTTGTGAGAT TTGTGACAGG ACCAGTGAGG GGACAGCAGC AACTGAACAA
    CCCAAGGCAC TTGATATGGC ACATGTGCAG GAGGAGATCC GGCAGAAGCG ACTAAGAGAG
    GATGGAGCTA AAATAGTGGC ATTGATCCGG GAAGGAGAGA AGTATGGGGT TCTACCTGAA
    GAGGTGTGTA GCGCAATACG ATATTCTGGT ACAGAAGTGC CTCAGTGGTG GCTGCAGACT
    GAACTTCCCT ATGTATTAGA GCGTCTGCTG GATGCTGCTT CACAGAAAGC AGGAGATACA
    ATTGGTGCCC TAACAGTGGA AGAAGCTCGT GATGCTTGGG TGTCCAGTGG TGGAGACATG
    GATGCTGCTG TCATCTTATG TGTGACAGAG CGAAGAAGGA AGGTTGATGC ACTCTCCGGC
    CTAGGTTTTC CAGAAAGGCA TAAAGTGGTA GCTGCTCTTT ATGAAAGTGC TGGAGATGTT
    GGTAGAGCAC TAAGTATATT ACAGAGACCA CTTCTGGAGC CTTTTCTTAT ACGCATGTGG
    GAAGAACCAC AACCACCCCT TCGGCTAGAC TCAGCAGATA GACAGGCAGT TCTCCGTAGG
    TTACTTGCAG AACATTCCTT GCACAGTTGG GGTCGGGCAG AGCTAGCTTT GTCATTGCTT
    ATTGAAGGAG AAGGGCGCTA TGAACTCCAG GATGTTGTAG AGGCAGTGCG GGAATCGCAG
    GACAGAGACT TTATCAAAAG GATGTTAACA CAGGAGTGTG CAGTGTGTGG ATGGGAGCTC
    CCCCGTAACA AGATGCGTTC TCTAACCTCA TGTGAGTGTT GTATCTGTCC TGATTGCTTC
    CGGATGCATT TCACTGTAGC AGTGAAAGAG AAACATATTA GAGACATGAT ATGTCCAGCT
    TGTGAGGAGC CAGAAATAAG CGACGAGGGG GAGCTTCTTC ACTATTTCTC TACTCTGGAT
    ATCCTGTTAC GGGACACTTT GGAGGTTGAT GCATATGATT TGTTCCATAA AAAACTAACT
    GAAAGAACAC TTATGAAGGA CCCAAAGTTC CTGTGGTGTA CACATTGTTC CTTTGGATTC
    ATTTATGAAC GAGATCAGCT GGATGTAAAA TGCCCCCAGT GTCAGTGCAG TTTCTGCAGG
    AAATGCAAGA GGCCTTGGGA AGAACAGCAT CGGTCTTTAA ATTGTGATGA TTTTCAGAAC
    TGGAAAAGGG AAAATGATGC AGAATACCAA GCTCAAGGAT TGGCTGTATA CCTGCTAGAG
    AATGGGATCA CCTGTCCCCA TTGTAAATTT TCTTATGCTC TTGCACGAGG GGGATGCATG
    CACTTCATAT GTTCTCAGTG TCGCCACCAG TTCTGCAGTG GCTGCTATAA CACCTTTCAC
    CAAAGAAATA AATGTACAGT CCCGTCCTGT ACGGTAAGGA TGTCTCTCCA TGCTCACCAT
    CCTAGAGACT GTCTCTTTTA CCTCCGAGAT TGGGATGTCA CACGTCTGCA AAGACTACTC
    CAGATGTTAC TGGTACAGTT TAACACAGAT CCACCCGCTG GAACACAAAC TGCTCCAGGA
    GGTGGGTGTC GTGTGATGGA GCAAAAGGAG ATACCTGATG GCCTGAGAGA CGAAGCTTGT
    GGCAAAATAA CACCTCCAGG ATATGCTGGT TTGTGCGAGT CTCATTATAA AGAATATCTT
    GTTAGTCGTA TTAATGCCCA TTCTCTGGAT CCATGTGTTC TCTATGATCT GGCTGAGACT
    ATGAATGTGT GTCAGCGCTA CCTGCCTGTA TTGCCTCTAC AAGATCCCAC CGAGAATGAG
    GCCAGCTACA GGGAGCGTCT CCTGGGGGTG TTAATATCAG AGGTGCCACT GAGTATGAAC
    ATTCCACGGA AGCAGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47344
    Clone ID Related Accession (Same CDS sequence) XM_031899717.1 , XM_031899674.1
    Accession Version XM_031899717.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase RNF31 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTTGGGA CCTGGGGTTT TCCAGAAATG GAAGAACATC CTATAAGGAG TGCTCTGGAA 
    AATGCATTAA TTAATCAACC GGGGGCTGTG ACTCCTGAAT TCCTGAATAA AATGTTGTAT
    TTGCCTTTGA GAGAACGGTA TAGAAGACTA AGTGTGGAGG ATCTACTCAA AGGAGGGACA
    CAGGTATCAC GTCTACACTC TGTGACCACT GCTCTCAACA TCCTTGAGAA GTATGGGAGG
    AATCTGTTGT CCCCGCTTCG ACCCAAATTC TGGAGGAGTG TGAAATTTAA CAACCCTGTT
    TTCAAGACAA CAGTAGATGC CATTGAGGGT GGACGATTTG TTCTGTCTCT TTATGGCTAC
    TCTCAGTCAC TTCCTGATGG TCTAAGTTTT CCAGATTCTG TGCAGGAACC AGATGTCTCC
    ACTGTTGCTG CTGTAACAGC TGATGTGATT ATTCTTCACC ATGAACTCAA CCTACTCATC
    TCGAACATGC ACCCACGGCA CGAGGAAGCA GCACAAGAAC TATTAGGTGG TGAACTGCCA
    CAGCAGTCTC TTGTTGCTGA TTACGTAAGT GCTCCTAAAG ATCCACAACC AGAAACCACT
    TCTGCTGGCT GTTTTCTTTG TGGTACTCTA GTTTGTTCTG TGTTTTGTTC CTCTTGTAAT
    GAGATGCTCT GTGAGGAGTG TGATAAGAGA GCACACTCTC ATCCAGTCCG TGCCCAACAT
    GTGCGTTTAC CATGTGGAAA TACAGCAAAG CCCAGGTCAT TGGATCAGGA CTCCCCTTCT
    ACAGTGTCAT CTCGCCCTCC TTGGAGATGT TACTCTTGCC ATTTTGAAAA CAAAGGGGGT
    TCTGTTCTGT GTGTGGTATG TGAGCGCCCC CGTGGCTGTA CCAGCAGCCT TTCAAACCTA
    TCACTTGGAA GTACTTTACA GAGAGACAGC TGGGAATGCC AGGCTTGTAC TCTGCAAAAT
    TCTGCATCTG CAGTTCTGTG TGCAGTGTGT GATCGTCCCC GATTGGCAAG CAAACCAGCC
    CAGGATGGTT ACGATTCTGT TGCTTTGCTT TTGAGAGATA TACAGAGGGA TAACAAGAGT
    ATCACATGCC CTGTGAGTGA AAAAAATGGT CTATTTGCCA TGAAGAATCA TCAGGAGAGT
    ATGCATGAAC CAGAAAGCAT TGGTCCTCTG ATAGATCATA CAAGGCAAGA AGAGGAGATT
    GCTAAAAGTG AGGGCTGGCA GTGTTCCCAT TGCACTTTCT TTAACACACG AAATGGACGT
    GTTTGTGAGA TTTGTGACAG GACCAGTGAG GGGACAGCAG CAACTGAACA ACCCAAGGCA
    CTTGATATGG CACATGTGCA GGAGGAGATC CGGCAGAAGC GACTAAGAGA GGATGGAGCT
    AAAATAGTGG CATTGATCCG GGAAGGAGAG AAGTATGGGG TTCTACCTGA AGAGGTGTGT
    AGCGCAATAC GATATTCTGG TACAGAAGTG CCTCAGTGGT GGCTGCAGAC TGAACTTCCC
    TATGTATTAG AGCGTCTGCT GGATGCTGCT TCACAGAAAG CAGGAGATAC AATTGGTGCC
    CTAACAGTGG AAGAAGCTCG TGATGCTTGG GTGTCCAGTG GTGGAGACAT GGATGCTGCT
    GTCATCTTAT GTGTGACAGA GCGAAGAAGG AAGGTTGATG CACTCTCCGG CCTAGGTTTT
    CCAGAAAGGC ATAAAGTGGT AGCTGCTCTT TATGAAAGTG CTGGAGATGT TGGTAGAGCA
    CTAAGTATAT TACAGAGACC ACTTCTGGAG CCTTTTCTTA TACGCATGTG GGAAGAACCA
    CAACCACCCC TTCGGCTAGA CTCGGCAGAT AGACAGGCAG TTCTCCGTAG GTTACTTGCA
    GAACATTCCT TGCACAGTTG GGGTCGGGCA GAGCTAGCTT TGTCATTGCT TATTGAAGGA
    GAAGGGCGCT ATGAACTCCA GGATGTTGTA GAGGCAGTGC GGGAATCGCA GGACAGAGAC
    TTTATCAAAA GGATGTTAAC ACAGGAGTGT GCAGTGTGTG GATGGGAGCT CCCCCGTAAC
    AAGATGCGTT CTCTAACCTC ATGTGAGTGT TGTATCTGTC CTGATTGCTT CCGGATGCAT
    TTCACTGTAG CAGTGAAAGA GAAACATATT AGAGACATGA TATGTCCAGC TTGTGAGGAG
    CCAGAAATAA GCGACGAGGG GGAGCTTCTT CACTATTTCT CTACTCTGGA TATCCTGTTA
    CGGGACACTT TGGAGGTTGA TGCATATGAT TTGTTCCATA AAAAACTAAC TGAAAGAACA
    CTTATGAAGG ACCCAAAGTT CCTGTGGTGT ACACATTGTT CCTTTGGATT CATTTATGAA
    CGAGATCAGC TGGATGTAAA ATGCCCCCAG TGTCAGTGCA GTTTCTGCAG GAAATGCAAG
    AGGCCTTGGG AAGAACAGCA TCGGTCTTTA AATTGTGATG ATTTTCAGAA CTGGAAAAGG
    GAAAATGATG CAGAATACCA AGCTCAAGGA TTGGCTGTAT ACCTGCTAGA GAATGGGATC
    ACCTGTCCCC ATTGTAAATT TTCTTATGCT CTTGCACGAG GGGGATGCAT GCACTTCATA
    TGTTCTCAGT GTCGCCACCA GTTCTGCAGT GGCTGCTATA ACACCTTTCA CCAAAGAAAT
    AAATGTACAG TCCCGTCCTG TACGGTAAGG ATGTCTCTCC ATGCTCACCA TCCTAGAGAC
    TGTCTCTTTT ACCTCCGAGA TTGGGATGTC ACACGTCTGC AAAGACTACT CCAGATGTTA
    CTGGTACAGT TTAACACAGA TCCACCCGCT GGAACACAAA CTGCTCCAGG AGGTGGGTGT
    CGTGTGATGG AGCAAAAGGA GATACCTGAT GGCCTGAGAG ACGAAGCTTG TGGCAAAATA
    ACACCTCCAG GATATGCTGG TTTGTGCGAG TCTCATTATA AAGAATATCT TGTTAGTCGT
    ATTAATGCCC ATTCTCTGGA TCCATGTGTT CTCTATGATC TGGCTGAGAC TATGAATGTG
    TGTCAGCGCT ACCTGCCTGT ATTGCCTCTA CAAGATCCCA CCGAGGATGA GGCCAGCTAC
    AGGGAGCGTC TCCTGGGGGT GTTAATATCA GAGGTGCCAC TGAGTATGAA CATTCCACGG
    AAGCAGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755577.1
    CDS508..3639
    Translation

    Target ORF information:

    RefSeq Version XM_031899717.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899717.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTTGGGA CCTGGGGTTT TCCAGAAATG GAAGAACATC CTATAAGGAG TGCTCTGGAA 
    AATGCATTAA TTAATCAACC GGGGGCTGTG ACTCCTGAAT TCCTGAATAA AATGTTGTAT
    TTGCCTTTGA GAGAACGGTA TAGAAGACTA AGTGTGGAGG ATCTACTCAA AGGAGGGACA
    CAGGTATCAC GTCTACACTC TGTGACCACT GCTCTCAACA TCCTTGAGAA GTATGGGAGG
    AATCTGTTGT CCCCGCTTCG ACCCAAATTC TGGAGGAGTG TGAAATTTAA CAACCCTGTT
    TTCAAGACAA CAGTAGATGC CATTGAGGGT GGACGATTTG TTCTGTCTCT TTATGGCTAC
    TCTCAGTCAC TTCCTGATGG TCTAAGTTTT CCAGATTCTG TGCAGGAACC AGATGTCTCC
    ACTGTTGCTG CTGTAACAGC TGATGTGATT ATTCTTCACC ATGAACTCAA CCTACTCATC
    TCGAACATGC ACCCACGGCA CGAGGAAGCA GCACAAGAAC TATTAGGTGG TGAACTGCCA
    CAGCAGTCTC TTGTTGCTGA TTACGTAAGT GCTCCTAAAG ATCCACAACC AGAAACCACT
    TCTGCTGGCT GTTTTCTTTG TGGTACTCTA GTTTGTTCTG TGTTTTGTTC CTCTTGTAAT
    GAGATGCTCT GTGAGGAGTG TGATAAGAGA GCACACTCTC ATCCAGTCCG TGCCCAACAT
    GTGCGTTTAC CATGTGGAAA TACAGCAAAG CCCAGGTCAT TGGATCAGGA CTCCCCTTCT
    ACAGTGTCAT CTCGCCCTCC TTGGAGATGT TACTCTTGCC ATTTTGAAAA CAAAGGGGGT
    TCTGTTCTGT GTGTGGTATG TGAGCGCCCC CGTGGCTGTA CCAGCAGCCT TTCAAACCTA
    TCACTTGGAA GTACTTTACA GAGAGACAGC TGGGAATGCC AGGCTTGTAC TCTGCAAAAT
    TCTGCATCTG CAGTTCTGTG TGCAGTGTGT GATCGTCCCC GATTGGCAAG CAAACCAGCC
    CAGGATGGTT ACGATTCTGT TGCTTTGCTT TTGAGAGATA TACAGAGGGA TAACAAGAGT
    ATCACATGCC CTGTGAGTGA AAAAAATGGT CTATTTGCCA TGAAGAATCA TCAGGAGAGT
    ATGCATGAAC CAGAAAGCAT TGGTCCTCTG ATAGATCATA CAAGGCAAGA AGAGGAGATT
    GCTAAAAGTG AGGGCTGGCA GTGTTCCCAT TGCACTTTCT TTAACACACG AAATGGACGT
    GTTTGTGAGA TTTGTGACAG GACCAGTGAG GGGACAGCAG CAACTGAACA ACCCAAGGCA
    CTTGATATGG CACATGTGCA GGAGGAGATC CGGCAGAAGC GACTAAGAGA GGATGGAGCT
    AAAATAGTGG CATTGATCCG GGAAGGAGAG AAGTATGGGG TTCTACCTGA AGAGGTGTGT
    AGCGCAATAC GATATTCTGG TACAGAAGTG CCTCAGTGGT GGCTGCAGAC TGAACTTCCC
    TATGTATTAG AGCGTCTGCT GGATGCTGCT TCACAGAAAG CAGGAGATAC AATTGGTGCC
    CTAACAGTGG AAGAAGCTCG TGATGCTTGG GTGTCCAGTG GTGGAGACAT GGATGCTGCT
    GTCATCTTAT GTGTGACAGA GCGAAGAAGG AAGGTTGATG CACTCTCCGG CCTAGGTTTT
    CCAGAAAGGC ATAAAGTGGT AGCTGCTCTT TATGAAAGTG CTGGAGATGT TGGTAGAGCA
    CTAAGTATAT TACAGAGACC ACTTCTGGAG CCTTTTCTTA TACGCATGTG GGAAGAACCA
    CAACCACCCC TTCGGCTAGA CTCGGCAGAT AGACAGGCAG TTCTCCGTAG GTTACTTGCA
    GAACATTCCT TGCACAGTTG GGGTCGGGCA GAGCTAGCTT TGTCATTGCT TATTGAAGGA
    GAAGGGCGCT ATGAACTCCA GGATGTTGTA GAGGCAGTGC GGGAATCGCA GGACAGAGAC
    TTTATCAAAA GGATGTTAAC ACAGGAGTGT GCAGTGTGTG GATGGGAGCT CCCCCGTAAC
    AAGATGCGTT CTCTAACCTC ATGTGAGTGT TGTATCTGTC CTGATTGCTT CCGGATGCAT
    TTCACTGTAG CAGTGAAAGA GAAACATATT AGAGACATGA TATGTCCAGC TTGTGAGGAG
    CCAGAAATAA GCGACGAGGG GGAGCTTCTT CACTATTTCT CTACTCTGGA TATCCTGTTA
    CGGGACACTT TGGAGGTTGA TGCATATGAT TTGTTCCATA AAAAACTAAC TGAAAGAACA
    CTTATGAAGG ACCCAAAGTT CCTGTGGTGT ACACATTGTT CCTTTGGATT CATTTATGAA
    CGAGATCAGC TGGATGTAAA ATGCCCCCAG TGTCAGTGCA GTTTCTGCAG GAAATGCAAG
    AGGCCTTGGG AAGAACAGCA TCGGTCTTTA AATTGTGATG ATTTTCAGAA CTGGAAAAGG
    GAAAATGATG CAGAATACCA AGCTCAAGGA TTGGCTGTAT ACCTGCTAGA GAATGGGATC
    ACCTGTCCCC ATTGTAAATT TTCTTATGCT CTTGCACGAG GGGGATGCAT GCACTTCATA
    TGTTCTCAGT GTCGCCACCA GTTCTGCAGT GGCTGCTATA ACACCTTTCA CCAAAGAAAT
    AAATGTACAG TCCCGTCCTG TACGGTAAGG ATGTCTCTCC ATGCTCACCA TCCTAGAGAC
    TGTCTCTTTT ACCTCCGAGA TTGGGATGTC ACACGTCTGC AAAGACTACT CCAGATGTTA
    CTGGTACAGT TTAACACAGA TCCACCCGCT GGAACACAAA CTGCTCCAGG AGGTGGGTGT
    CGTGTGATGG AGCAAAAGGA GATACCTGAT GGCCTGAGAG ACGAAGCTTG TGGCAAAATA
    ACACCTCCAG GATATGCTGG TTTGTGCGAG TCTCATTATA AAGAATATCT TGTTAGTCGT
    ATTAATGCCC ATTCTCTGGA TCCATGTGTT CTCTATGATC TGGCTGAGAC TATGAATGTG
    TGTCAGCGCT ACCTGCCTGT ATTGCCTCTA CAAGATCCCA CCGAGGATGA GGCCAGCTAC
    AGGGAGCGTC TCCTGGGGGT GTTAATATCA GAGGTGCCAC TGAGTATGAA CATTCCACGG
    AAGCAGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47344
    Clone ID Related Accession (Same CDS sequence) XM_031899717.1 , XM_031899674.1
    Accession Version XM_031899674.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase RNF31 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTTGGGA CCTGGGGTTT TCCAGAAATG GAAGAACATC CTATAAGGAG TGCTCTGGAA 
    AATGCATTAA TTAATCAACC GGGGGCTGTG ACTCCTGAAT TCCTGAATAA AATGTTGTAT
    TTGCCTTTGA GAGAACGGTA TAGAAGACTA AGTGTGGAGG ATCTACTCAA AGGAGGGACA
    CAGGTATCAC GTCTACACTC TGTGACCACT GCTCTCAACA TCCTTGAGAA GTATGGGAGG
    AATCTGTTGT CCCCGCTTCG ACCCAAATTC TGGAGGAGTG TGAAATTTAA CAACCCTGTT
    TTCAAGACAA CAGTAGATGC CATTGAGGGT GGACGATTTG TTCTGTCTCT TTATGGCTAC
    TCTCAGTCAC TTCCTGATGG TCTAAGTTTT CCAGATTCTG TGCAGGAACC AGATGTCTCC
    ACTGTTGCTG CTGTAACAGC TGATGTGATT ATTCTTCACC ATGAACTCAA CCTACTCATC
    TCGAACATGC ACCCACGGCA CGAGGAAGCA GCACAAGAAC TATTAGGTGG TGAACTGCCA
    CAGCAGTCTC TTGTTGCTGA TTACGTAAGT GCTCCTAAAG ATCCACAACC AGAAACCACT
    TCTGCTGGCT GTTTTCTTTG TGGTACTCTA GTTTGTTCTG TGTTTTGTTC CTCTTGTAAT
    GAGATGCTCT GTGAGGAGTG TGATAAGAGA GCACACTCTC ATCCAGTCCG TGCCCAACAT
    GTGCGTTTAC CATGTGGAAA TACAGCAAAG CCCAGGTCAT TGGATCAGGA CTCCCCTTCT
    ACAGTGTCAT CTCGCCCTCC TTGGAGATGT TACTCTTGCC ATTTTGAAAA CAAAGGGGGT
    TCTGTTCTGT GTGTGGTATG TGAGCGCCCC CGTGGCTGTA CCAGCAGCCT TTCAAACCTA
    TCACTTGGAA GTACTTTACA GAGAGACAGC TGGGAATGCC AGGCTTGTAC TCTGCAAAAT
    TCTGCATCTG CAGTTCTGTG TGCAGTGTGT GATCGTCCCC GATTGGCAAG CAAACCAGCC
    CAGGATGGTT ACGATTCTGT TGCTTTGCTT TTGAGAGATA TACAGAGGGA TAACAAGAGT
    ATCACATGCC CTGTGAGTGA AAAAAATGGT CTATTTGCCA TGAAGAATCA TCAGGAGAGT
    ATGCATGAAC CAGAAAGCAT TGGTCCTCTG ATAGATCATA CAAGGCAAGA AGAGGAGATT
    GCTAAAAGTG AGGGCTGGCA GTGTTCCCAT TGCACTTTCT TTAACACACG AAATGGACGT
    GTTTGTGAGA TTTGTGACAG GACCAGTGAG GGGACAGCAG CAACTGAACA ACCCAAGGCA
    CTTGATATGG CACATGTGCA GGAGGAGATC CGGCAGAAGC GACTAAGAGA GGATGGAGCT
    AAAATAGTGG CATTGATCCG GGAAGGAGAG AAGTATGGGG TTCTACCTGA AGAGGTGTGT
    AGCGCAATAC GATATTCTGG TACAGAAGTG CCTCAGTGGT GGCTGCAGAC TGAACTTCCC
    TATGTATTAG AGCGTCTGCT GGATGCTGCT TCACAGAAAG CAGGAGATAC AATTGGTGCC
    CTAACAGTGG AAGAAGCTCG TGATGCTTGG GTGTCCAGTG GTGGAGACAT GGATGCTGCT
    GTCATCTTAT GTGTGACAGA GCGAAGAAGG AAGGTTGATG CACTCTCCGG CCTAGGTTTT
    CCAGAAAGGC ATAAAGTGGT AGCTGCTCTT TATGAAAGTG CTGGAGATGT TGGTAGAGCA
    CTAAGTATAT TACAGAGACC ACTTCTGGAG CCTTTTCTTA TACGCATGTG GGAAGAACCA
    CAACCACCCC TTCGGCTAGA CTCGGCAGAT AGACAGGCAG TTCTCCGTAG GTTACTTGCA
    GAACATTCCT TGCACAGTTG GGGTCGGGCA GAGCTAGCTT TGTCATTGCT TATTGAAGGA
    GAAGGGCGCT ATGAACTCCA GGATGTTGTA GAGGCAGTGC GGGAATCGCA GGACAGAGAC
    TTTATCAAAA GGATGTTAAC ACAGGAGTGT GCAGTGTGTG GATGGGAGCT CCCCCGTAAC
    AAGATGCGTT CTCTAACCTC ATGTGAGTGT TGTATCTGTC CTGATTGCTT CCGGATGCAT
    TTCACTGTAG CAGTGAAAGA GAAACATATT AGAGACATGA TATGTCCAGC TTGTGAGGAG
    CCAGAAATAA GCGACGAGGG GGAGCTTCTT CACTATTTCT CTACTCTGGA TATCCTGTTA
    CGGGACACTT TGGAGGTTGA TGCATATGAT TTGTTCCATA AAAAACTAAC TGAAAGAACA
    CTTATGAAGG ACCCAAAGTT CCTGTGGTGT ACACATTGTT CCTTTGGATT CATTTATGAA
    CGAGATCAGC TGGATGTAAA ATGCCCCCAG TGTCAGTGCA GTTTCTGCAG GAAATGCAAG
    AGGCCTTGGG AAGAACAGCA TCGGTCTTTA AATTGTGATG ATTTTCAGAA CTGGAAAAGG
    GAAAATGATG CAGAATACCA AGCTCAAGGA TTGGCTGTAT ACCTGCTAGA GAATGGGATC
    ACCTGTCCCC ATTGTAAATT TTCTTATGCT CTTGCACGAG GGGGATGCAT GCACTTCATA
    TGTTCTCAGT GTCGCCACCA GTTCTGCAGT GGCTGCTATA ACACCTTTCA CCAAAGAAAT
    AAATGTACAG TCCCGTCCTG TACGGTAAGG ATGTCTCTCC ATGCTCACCA TCCTAGAGAC
    TGTCTCTTTT ACCTCCGAGA TTGGGATGTC ACACGTCTGC AAAGACTACT CCAGATGTTA
    CTGGTACAGT TTAACACAGA TCCACCCGCT GGAACACAAA CTGCTCCAGG AGGTGGGTGT
    CGTGTGATGG AGCAAAAGGA GATACCTGAT GGCCTGAGAG ACGAAGCTTG TGGCAAAATA
    ACACCTCCAG GATATGCTGG TTTGTGCGAG TCTCATTATA AAGAATATCT TGTTAGTCGT
    ATTAATGCCC ATTCTCTGGA TCCATGTGTT CTCTATGATC TGGCTGAGAC TATGAATGTG
    TGTCAGCGCT ACCTGCCTGT ATTGCCTCTA CAAGATCCCA CCGAGGATGA GGCCAGCTAC
    AGGGAGCGTC TCCTGGGGGT GTTAATATCA GAGGTGCCAC TGAGTATGAA CATTCCACGG
    AAGCAGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755534.1
    CDS535..3666
    Translation

    Target ORF information:

    RefSeq Version XM_031899674.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899674.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTTGGGA CCTGGGGTTT TCCAGAAATG GAAGAACATC CTATAAGGAG TGCTCTGGAA 
    AATGCATTAA TTAATCAACC GGGGGCTGTG ACTCCTGAAT TCCTGAATAA AATGTTGTAT
    TTGCCTTTGA GAGAACGGTA TAGAAGACTA AGTGTGGAGG ATCTACTCAA AGGAGGGACA
    CAGGTATCAC GTCTACACTC TGTGACCACT GCTCTCAACA TCCTTGAGAA GTATGGGAGG
    AATCTGTTGT CCCCGCTTCG ACCCAAATTC TGGAGGAGTG TGAAATTTAA CAACCCTGTT
    TTCAAGACAA CAGTAGATGC CATTGAGGGT GGACGATTTG TTCTGTCTCT TTATGGCTAC
    TCTCAGTCAC TTCCTGATGG TCTAAGTTTT CCAGATTCTG TGCAGGAACC AGATGTCTCC
    ACTGTTGCTG CTGTAACAGC TGATGTGATT ATTCTTCACC ATGAACTCAA CCTACTCATC
    TCGAACATGC ACCCACGGCA CGAGGAAGCA GCACAAGAAC TATTAGGTGG TGAACTGCCA
    CAGCAGTCTC TTGTTGCTGA TTACGTAAGT GCTCCTAAAG ATCCACAACC AGAAACCACT
    TCTGCTGGCT GTTTTCTTTG TGGTACTCTA GTTTGTTCTG TGTTTTGTTC CTCTTGTAAT
    GAGATGCTCT GTGAGGAGTG TGATAAGAGA GCACACTCTC ATCCAGTCCG TGCCCAACAT
    GTGCGTTTAC CATGTGGAAA TACAGCAAAG CCCAGGTCAT TGGATCAGGA CTCCCCTTCT
    ACAGTGTCAT CTCGCCCTCC TTGGAGATGT TACTCTTGCC ATTTTGAAAA CAAAGGGGGT
    TCTGTTCTGT GTGTGGTATG TGAGCGCCCC CGTGGCTGTA CCAGCAGCCT TTCAAACCTA
    TCACTTGGAA GTACTTTACA GAGAGACAGC TGGGAATGCC AGGCTTGTAC TCTGCAAAAT
    TCTGCATCTG CAGTTCTGTG TGCAGTGTGT GATCGTCCCC GATTGGCAAG CAAACCAGCC
    CAGGATGGTT ACGATTCTGT TGCTTTGCTT TTGAGAGATA TACAGAGGGA TAACAAGAGT
    ATCACATGCC CTGTGAGTGA AAAAAATGGT CTATTTGCCA TGAAGAATCA TCAGGAGAGT
    ATGCATGAAC CAGAAAGCAT TGGTCCTCTG ATAGATCATA CAAGGCAAGA AGAGGAGATT
    GCTAAAAGTG AGGGCTGGCA GTGTTCCCAT TGCACTTTCT TTAACACACG AAATGGACGT
    GTTTGTGAGA TTTGTGACAG GACCAGTGAG GGGACAGCAG CAACTGAACA ACCCAAGGCA
    CTTGATATGG CACATGTGCA GGAGGAGATC CGGCAGAAGC GACTAAGAGA GGATGGAGCT
    AAAATAGTGG CATTGATCCG GGAAGGAGAG AAGTATGGGG TTCTACCTGA AGAGGTGTGT
    AGCGCAATAC GATATTCTGG TACAGAAGTG CCTCAGTGGT GGCTGCAGAC TGAACTTCCC
    TATGTATTAG AGCGTCTGCT GGATGCTGCT TCACAGAAAG CAGGAGATAC AATTGGTGCC
    CTAACAGTGG AAGAAGCTCG TGATGCTTGG GTGTCCAGTG GTGGAGACAT GGATGCTGCT
    GTCATCTTAT GTGTGACAGA GCGAAGAAGG AAGGTTGATG CACTCTCCGG CCTAGGTTTT
    CCAGAAAGGC ATAAAGTGGT AGCTGCTCTT TATGAAAGTG CTGGAGATGT TGGTAGAGCA
    CTAAGTATAT TACAGAGACC ACTTCTGGAG CCTTTTCTTA TACGCATGTG GGAAGAACCA
    CAACCACCCC TTCGGCTAGA CTCGGCAGAT AGACAGGCAG TTCTCCGTAG GTTACTTGCA
    GAACATTCCT TGCACAGTTG GGGTCGGGCA GAGCTAGCTT TGTCATTGCT TATTGAAGGA
    GAAGGGCGCT ATGAACTCCA GGATGTTGTA GAGGCAGTGC GGGAATCGCA GGACAGAGAC
    TTTATCAAAA GGATGTTAAC ACAGGAGTGT GCAGTGTGTG GATGGGAGCT CCCCCGTAAC
    AAGATGCGTT CTCTAACCTC ATGTGAGTGT TGTATCTGTC CTGATTGCTT CCGGATGCAT
    TTCACTGTAG CAGTGAAAGA GAAACATATT AGAGACATGA TATGTCCAGC TTGTGAGGAG
    CCAGAAATAA GCGACGAGGG GGAGCTTCTT CACTATTTCT CTACTCTGGA TATCCTGTTA
    CGGGACACTT TGGAGGTTGA TGCATATGAT TTGTTCCATA AAAAACTAAC TGAAAGAACA
    CTTATGAAGG ACCCAAAGTT CCTGTGGTGT ACACATTGTT CCTTTGGATT CATTTATGAA
    CGAGATCAGC TGGATGTAAA ATGCCCCCAG TGTCAGTGCA GTTTCTGCAG GAAATGCAAG
    AGGCCTTGGG AAGAACAGCA TCGGTCTTTA AATTGTGATG ATTTTCAGAA CTGGAAAAGG
    GAAAATGATG CAGAATACCA AGCTCAAGGA TTGGCTGTAT ACCTGCTAGA GAATGGGATC
    ACCTGTCCCC ATTGTAAATT TTCTTATGCT CTTGCACGAG GGGGATGCAT GCACTTCATA
    TGTTCTCAGT GTCGCCACCA GTTCTGCAGT GGCTGCTATA ACACCTTTCA CCAAAGAAAT
    AAATGTACAG TCCCGTCCTG TACGGTAAGG ATGTCTCTCC ATGCTCACCA TCCTAGAGAC
    TGTCTCTTTT ACCTCCGAGA TTGGGATGTC ACACGTCTGC AAAGACTACT CCAGATGTTA
    CTGGTACAGT TTAACACAGA TCCACCCGCT GGAACACAAA CTGCTCCAGG AGGTGGGTGT
    CGTGTGATGG AGCAAAAGGA GATACCTGAT GGCCTGAGAG ACGAAGCTTG TGGCAAAATA
    ACACCTCCAG GATATGCTGG TTTGTGCGAG TCTCATTATA AAGAATATCT TGTTAGTCGT
    ATTAATGCCC ATTCTCTGGA TCCATGTGTT CTCTATGATC TGGCTGAGAC TATGAATGTG
    TGTCAGCGCT ACCTGCCTGT ATTGCCTCTA CAAGATCCCA CCGAGGATGA GGCCAGCTAC
    AGGGAGCGTC TCCTGGGGGT GTTAATATCA GAGGTGCCAC TGAGTATGAA CATTCCACGG
    AAGCAGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47345
    Clone ID Related Accession (Same CDS sequence) XM_031899951.1
    Accession Version XM_031899951.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase RNF31 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTTGGGA CCTGGGGTTT TCCAGAAATG GAAGAACATC CTATAAGGAG TGCTCTGGAA 
    AATGCATTAA TTAATCAACC GGGGGCTGTG ACTCCTGAAT TCCTGAATAA AATGTTGTAT
    TTGCCTTTGA GAGAACGGTA TAGAAGACTA AGTGTGGAGG ATCTACTCAA AGGAGGGACA
    CAGGTATCAC GTCTACACTC TGTGACCACT GCTCTCAACA TCCTTGAGAA GTATGGGAGG
    AATCTGTTGT CCCCGCTTCG ACCCAAATTC TGGAGGAGTG TGAAATTTAA CAACCCTGTT
    TTCAAGACAA CAGTAGATGC CATTGAGGGT GGACGATTTG TTCTGTCTCT TTATGGCTAC
    TCTCAGTCAC TTCCTGATGG TCTAAGTTTT CCAGATTCTG TGCAGGAACC AGATGTCTCC
    ACTGTTGCTG CTGTAACAGC TGATGTGATT ATTCTTCACC ATGAACTCAA CCTACTCATC
    TCGAACATGC ACCCACGGCA CGAGGAAGCA GCACAAGAAC TATTAGGTGG TGAACTGCCA
    CAGTCTCTTG TTGCTGATTA CGTAAGTGCT CCTAAAGATC CACAACCAGA AACCACTTCT
    GCTGGCTGTT TTCTTTGTGG TACTCTAGTT TGTTCTGTGT TTTGTTCCTC TTGTAATGAG
    ATGCTCTGTG AGGAGTGTGA TAAGAGAGCA CACTCTCATC CAGTCCGTGC CCAACATGTG
    CGTTTACCAT GTGGAAATAC AGCAAAGCCC AGGTCATTGG ATCAGGACTC CCCTTCTACA
    GTGTCATCTC GCCCTCCTTG GAGATGTTAC TCTTGCCATT TTGAAAACAA AGGGGGTTCT
    GTTCTGTGTG TGGTATGTGA GCGCCCCCGT GGCTGTACCA GCAGCCTTTC AAACCTATCA
    CTTGGAAGTA CTTTACAGAG AGACAGCTGG GAATGCCAGG CTTGTACTCT GCAAAATTCT
    GCATCTGCAG TTCTGTGTGC AGTGTGTGAT CGTCCCCGAT TGGCAAGCAA ACCAGCCCAG
    GATGGTTACG ATTCTGTTGC TTTGCTTTTG AGAGATATAC AGAGGGATAA CAAGAGTATC
    ACATGCCCTG TGAGTGAAAA AAATGGTCTA TTTGCCATGA AGAATCATCA GGAGAGTATG
    CATGAACCAG AAAGCATTGG TCCTCTGATA GATCATACAA GGCAAGAAGA GGAGATTGCT
    AAAAGTGAGG GCTGGCAGTG TTCCCATTGC ACTTTCTTTA ACACACGAAA TGGACGTGTT
    TGTGAGATTT GTGACAGGAC CAGTGAGGGG ACAGCAGCAA CTGAACAACC CAAGGCACTT
    GATATGGCAC ATGTGCAGGA GGAGATCCGG CAGAAGCGAC TAAGAGAGGA TGGAGCTAAA
    ATAGTGGCAT TGATCCGGGA AGGAGAGAAG TATGGGGTTC TACCTGAAGA GGTGTGTAGC
    GCAATACGAT ATTCTGGTAC AGAAGTGCCT CAGTGGTGGC TGCAGACTGA ACTTCCCTAT
    GTATTAGAGC GTCTGCTGGA TGCTGCTTCA CAGAAAGCAG GAGATACAAT TGGTGCCCTA
    ACAGTGGAAG AAGCTCGTGA TGCTTGGGTG TCCAGTGGTG GAGACATGGA TGCTGCTGTC
    ATCTTATGTG TGACAGAGCG AAGAAGGAAG GTTGATGCAC TCTCCGGCCT AGGTTTTCCA
    GAAAGGCATA AAGTGGTAGC TGCTCTTTAT GAAAGTGCTG GAGATGTTGG TAGAGCACTA
    AGTATATTAC AGAGACCACT TCTGGAGCCT TTTCTTATAC GCATGTGGGA AGAACCACAA
    CCACCCCTTC GGCTAGACTC GGCAGATAGA CAGGCAGTTC TCCGTAGGTT ACTTGCAGAA
    CATTCCTTGC ACAGTTGGGG TCGGGCAGAG CTAGCTTTGT CATTGCTTAT TGAAGGAGAA
    GGGCGCTATG AACTCCAGGA TGTTGTAGAG GCAGTGCGGG AATCGCAGGA CAGAGACTTT
    ATCAAAAGGA TGTTAACACA GGAGTGTGCA GTGTGTGGAT GGGAGCTCCC CCGTAACAAG
    ATGCGTTCTC TAACCTCATG TGAGTGTTGT ATCTGTCCTG ATTGCTTCCG GATGCATTTC
    ACTGTAGCAG TGAAAGAGAA ACATATTAGA GACATGATAT GTCCAGCTTG TGAGGAGCCA
    GAAATAAGCG ACGAGGGGGA GCTTCTTCAC TATTTCTCTA CTCTGGATAT CCTGTTACGG
    GACACTTTGG AGGTTGATGC ATATGATTTG TTCCATAAAA AACTAACTGA AAGAACACTT
    ATGAAGGACC CAAAGTTCCT GTGGTGTACA CATTGTTCCT TTGGATTCAT TTATGAACGA
    GATCAGCTGG ATGTAAAATG CCCCCAGTGT CAGTGCAGTT TCTGCAGGAA ATGCAAGAGG
    CCTTGGGAAG AACAGCATCG GTCTTTAAAT TGTGATGATT TTCAGAACTG GAAAAGGGAA
    AATGATGCAG AATACCAAGC TCAAGGATTG GCTGTATACC TGCTAGAGAA TGGGATCACC
    TGTCCCCATT GTAAATTTTC TTATGCTCTT GCACGAGGGG GATGCATGCA CTTCATATGT
    TCTCAGTGTC GCCACCAGTT CTGCAGTGGC TGCTATAACA CCTTTCACCA AAGAAATAAA
    TGTACAGTCC CGTCCTGTAC GGTAAGGATG TCTCTCCATG CTCACCATCC TAGAGACTGT
    CTCTTTTACC TCCGAGATTG GGATGTCACA CGTCTGCAAA GACTACTCCA GATGTTACTG
    GTACAGTTTA ACACAGATCC ACCCGCTGGA ACACAAACTG CTCCAGGAGG TGGGTGTCGT
    GTGATGGAGC AAAAGGAGAT ACCTGATGGC CTGAGAGACG AAGCTTGTGG CAAAATAACA
    CCTCCAGGAT ATGCTGGTTT GTGCGAGTCT CATTATAAAG AATATCTTGT TAGTCGTATT
    AATGCCCATT CTCTGGATCC ATGTGTTCTC TATGATCTGG CTGAGACTAT GAATGTGTGT
    CAGCGCTACC TGCCTGTATT GCCTCTACAA GATCCCACCG AGGATGAGGC CAGCTACAGG
    GAGCGTCTCC TGGGGGTGTT AATATCAGAG GTGCCACTGA GTATGAACAT TCCACGGAAG
    CAGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755811.1
    CDS535..3663
    Translation

    Target ORF information:

    RefSeq Version XM_031899951.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899951.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTTGGGA CCTGGGGTTT TCCAGAAATG GAAGAACATC CTATAAGGAG TGCTCTGGAA 
    AATGCATTAA TTAATCAACC GGGGGCTGTG ACTCCTGAAT TCCTGAATAA AATGTTGTAT
    TTGCCTTTGA GAGAACGGTA TAGAAGACTA AGTGTGGAGG ATCTACTCAA AGGAGGGACA
    CAGGTATCAC GTCTACACTC TGTGACCACT GCTCTCAACA TCCTTGAGAA GTATGGGAGG
    AATCTGTTGT CCCCGCTTCG ACCCAAATTC TGGAGGAGTG TGAAATTTAA CAACCCTGTT
    TTCAAGACAA CAGTAGATGC CATTGAGGGT GGACGATTTG TTCTGTCTCT TTATGGCTAC
    TCTCAGTCAC TTCCTGATGG TCTAAGTTTT CCAGATTCTG TGCAGGAACC AGATGTCTCC
    ACTGTTGCTG CTGTAACAGC TGATGTGATT ATTCTTCACC ATGAACTCAA CCTACTCATC
    TCGAACATGC ACCCACGGCA CGAGGAAGCA GCACAAGAAC TATTAGGTGG TGAACTGCCA
    CAGTCTCTTG TTGCTGATTA CGTAAGTGCT CCTAAAGATC CACAACCAGA AACCACTTCT
    GCTGGCTGTT TTCTTTGTGG TACTCTAGTT TGTTCTGTGT TTTGTTCCTC TTGTAATGAG
    ATGCTCTGTG AGGAGTGTGA TAAGAGAGCA CACTCTCATC CAGTCCGTGC CCAACATGTG
    CGTTTACCAT GTGGAAATAC AGCAAAGCCC AGGTCATTGG ATCAGGACTC CCCTTCTACA
    GTGTCATCTC GCCCTCCTTG GAGATGTTAC TCTTGCCATT TTGAAAACAA AGGGGGTTCT
    GTTCTGTGTG TGGTATGTGA GCGCCCCCGT GGCTGTACCA GCAGCCTTTC AAACCTATCA
    CTTGGAAGTA CTTTACAGAG AGACAGCTGG GAATGCCAGG CTTGTACTCT GCAAAATTCT
    GCATCTGCAG TTCTGTGTGC AGTGTGTGAT CGTCCCCGAT TGGCAAGCAA ACCAGCCCAG
    GATGGTTACG ATTCTGTTGC TTTGCTTTTG AGAGATATAC AGAGGGATAA CAAGAGTATC
    ACATGCCCTG TGAGTGAAAA AAATGGTCTA TTTGCCATGA AGAATCATCA GGAGAGTATG
    CATGAACCAG AAAGCATTGG TCCTCTGATA GATCATACAA GGCAAGAAGA GGAGATTGCT
    AAAAGTGAGG GCTGGCAGTG TTCCCATTGC ACTTTCTTTA ACACACGAAA TGGACGTGTT
    TGTGAGATTT GTGACAGGAC CAGTGAGGGG ACAGCAGCAA CTGAACAACC CAAGGCACTT
    GATATGGCAC ATGTGCAGGA GGAGATCCGG CAGAAGCGAC TAAGAGAGGA TGGAGCTAAA
    ATAGTGGCAT TGATCCGGGA AGGAGAGAAG TATGGGGTTC TACCTGAAGA GGTGTGTAGC
    GCAATACGAT ATTCTGGTAC AGAAGTGCCT CAGTGGTGGC TGCAGACTGA ACTTCCCTAT
    GTATTAGAGC GTCTGCTGGA TGCTGCTTCA CAGAAAGCAG GAGATACAAT TGGTGCCCTA
    ACAGTGGAAG AAGCTCGTGA TGCTTGGGTG TCCAGTGGTG GAGACATGGA TGCTGCTGTC
    ATCTTATGTG TGACAGAGCG AAGAAGGAAG GTTGATGCAC TCTCCGGCCT AGGTTTTCCA
    GAAAGGCATA AAGTGGTAGC TGCTCTTTAT GAAAGTGCTG GAGATGTTGG TAGAGCACTA
    AGTATATTAC AGAGACCACT TCTGGAGCCT TTTCTTATAC GCATGTGGGA AGAACCACAA
    CCACCCCTTC GGCTAGACTC GGCAGATAGA CAGGCAGTTC TCCGTAGGTT ACTTGCAGAA
    CATTCCTTGC ACAGTTGGGG TCGGGCAGAG CTAGCTTTGT CATTGCTTAT TGAAGGAGAA
    GGGCGCTATG AACTCCAGGA TGTTGTAGAG GCAGTGCGGG AATCGCAGGA CAGAGACTTT
    ATCAAAAGGA TGTTAACACA GGAGTGTGCA GTGTGTGGAT GGGAGCTCCC CCGTAACAAG
    ATGCGTTCTC TAACCTCATG TGAGTGTTGT ATCTGTCCTG ATTGCTTCCG GATGCATTTC
    ACTGTAGCAG TGAAAGAGAA ACATATTAGA GACATGATAT GTCCAGCTTG TGAGGAGCCA
    GAAATAAGCG ACGAGGGGGA GCTTCTTCAC TATTTCTCTA CTCTGGATAT CCTGTTACGG
    GACACTTTGG AGGTTGATGC ATATGATTTG TTCCATAAAA AACTAACTGA AAGAACACTT
    ATGAAGGACC CAAAGTTCCT GTGGTGTACA CATTGTTCCT TTGGATTCAT TTATGAACGA
    GATCAGCTGG ATGTAAAATG CCCCCAGTGT CAGTGCAGTT TCTGCAGGAA ATGCAAGAGG
    CCTTGGGAAG AACAGCATCG GTCTTTAAAT TGTGATGATT TTCAGAACTG GAAAAGGGAA
    AATGATGCAG AATACCAAGC TCAAGGATTG GCTGTATACC TGCTAGAGAA TGGGATCACC
    TGTCCCCATT GTAAATTTTC TTATGCTCTT GCACGAGGGG GATGCATGCA CTTCATATGT
    TCTCAGTGTC GCCACCAGTT CTGCAGTGGC TGCTATAACA CCTTTCACCA AAGAAATAAA
    TGTACAGTCC CGTCCTGTAC GGTAAGGATG TCTCTCCATG CTCACCATCC TAGAGACTGT
    CTCTTTTACC TCCGAGATTG GGATGTCACA CGTCTGCAAA GACTACTCCA GATGTTACTG
    GTACAGTTTA ACACAGATCC ACCCGCTGGA ACACAAACTG CTCCAGGAGG TGGGTGTCGT
    GTGATGGAGC AAAAGGAGAT ACCTGATGGC CTGAGAGACG AAGCTTGTGG CAAAATAACA
    CCTCCAGGAT ATGCTGGTTT GTGCGAGTCT CATTATAAAG AATATCTTGT TAGTCGTATT
    AATGCCCATT CTCTGGATCC ATGTGTTCTC TATGATCTGG CTGAGACTAT GAATGTGTGT
    CAGCGCTACC TGCCTGTATT GCCTCTACAA GATCCCACCG AGGATGAGGC CAGCTACAGG
    GAGCGTCTCC TGGGGGTGTT AATATCAGAG GTGCCACTGA GTATGAACAT TCCACGGAAG
    CAGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47346
    Clone ID Related Accession (Same CDS sequence) XM_012962713.3
    Accession Version XM_012962713.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase RNF31 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGTAT CACGTCTACA CTCTGTGACC
    ACTGCTCTCA ACATCCTTGA GAAGTATGGG AGGAATCTGT TGTCCCCGCT TCGACCCAAA
    TTCTGGAGGA GTGTGAAATT TAACAACCCT GTTTTCAAGA CAACAGTAGA TGCCATTGAG
    GGTGGACGAT TTGTTCTGTC TCTTTATGGC TACTCTCAGT CACTTCCTGA TGGTCTAAGT
    TTTCCAGATT CTGTGCAGGA ACCAGATGTC TCCACTGTTG CTGCTGTAAC AGCTGATGTG
    ATTATTCTTC ACCATGAACT CAACCTACTC ATCTCGAACA TGCACCCACG GCACGAGGAA
    GCAGCACAAG AACTATTAGG TGGTGAACTG CCACAGCAGT CTCTTGTTGC TGATTACGTA
    AGTGCTCCTA AAGATCCACA ACCAGAAACC ACTTCTGCTG GCTGTTTTCT TTGTGGTACT
    CTAGTTTGTT CTGTGTTTTG TTCCTCTTGT AATGAGATGC TCTGTGAGGA GTGTGATAAG
    AGAGCACACT CTCATCCAGT CCGTGCCCAA CATGTGCGTT TACCATGTGG AAATACAGCA
    AAGCCCAGGT CATTGGATCA GGACTCCCCT TCTACAGTGT CATCTCGCCC TCCTTGGAGA
    TGTTACTCTT GCCATTTTGA AAACAAAGGG GGTTCTGTTC TGTGTGTGGT ATGTGAGCGC
    CCCCGTGGCT GTACCAGCAG CCTTTCAAAC CTATCACTTG GAAGTACTTT ACAGAGAGAC
    AGCTGGGAAT GCCAGGCTTG TACTCTGCAA AATTCTGCAT CTGCAGTTCT GTGTGCAGTG
    TGTGATCGTC CCCGATTGGC AAGCAAACCA GCCCAGGATG GTTACGATTC TGTTGCTTTG
    CTTTTGAGAG ATATACAGAG GGATAACAAG AGTATCACAT GCCCTGTGAG TGAAAAAAAT
    GGTCTATTTG CCATGAAGAA TCATCAGGAG AGTATGCATG AACCAGAAAG CATTGGTCCT
    CTGATAGATC ATACAAGGCA AGAAGAGGAG ATTGCTAAAA GTGAGGGCTG GCAGTGTTCC
    CATTGCACTT TCTTTAACAC ACGAAATGGA CGTGTTTGTG AGATTTGTGA CAGGACCAGT
    GAGGGGACAG CAGCAACTGA ACAACCCAAG GCACTTGATA TGGCACATGT GCAGGAGGAG
    ATCCGGCAGA AGCGACTAAG AGAGGATGGA GCTAAAATAG TGGCATTGAT CCGGGAAGGA
    GAGAAGTATG GGGTTCTACC TGAAGAGGTG TGTAGCGCAA TACGATATTC TGGTACAGAA
    GTGCCTCAGT GGTGGCTGCA GACTGAACTT CCCTATGTAT TAGAGCGTCT GCTGGATGCT
    GCTTCACAGA AAGCAGGAGA TACAATTGGT GCCCTAACAG TGGAAGAAGC TCGTGATGCT
    TGGGTGTCCA GTGGTGGAGA CATGGATGCT GCTGTCATCT TATGTGTGAC AGAGCGAAGA
    AGGAAGGTTG ATGCACTCTC CGGCCTAGGT TTTCCAGAAA GGCATAAAGT GGTAGCTGCT
    CTTTATGAAA GTGCTGGAGA TGTTGGTAGA GCACTAAGTA TATTACAGAG ACCACTTCTG
    GAGCCTTTTC TTATACGCAT GTGGGAAGAA CCACAACCAC CCCTTCGGCT AGACTCGGCA
    GATAGACAGG CAGTTCTCCG TAGGTTACTT GCAGAACATT CCTTGCACAG TTGGGGTCGG
    GCAGAGCTAG CTTTGTCATT GCTTATTGAA GGAGAAGGGC GCTATGAACT CCAGGATGTT
    GTAGAGGCAG TGCGGGAATC GCAGGACAGA GACTTTATCA AAAGGATGTT AACACAGGAG
    TGTGCAGTGT GTGGATGGGA GCTCCCCCGT AACAAGATGC GTTCTCTAAC CTCATGTGAG
    TGTTGTATCT GTCCTGATTG CTTCCGGATG CATTTCACTG TAGCAGTGAA AGAGAAACAT
    ATTAGAGACA TGATATGTCC AGCTTGTGAG GAGCCAGAAA TAAGCGACGA GGGGGAGCTT
    CTTCACTATT TCTCTACTCT GGATATCCTG TTACGGGACA CTTTGGAGGT TGATGCATAT
    GATTTGTTCC ATAAAAAACT AACTGAAAGA ACACTTATGA AGGACCCAAA GTTCCTGTGG
    TGTACACATT GTTCCTTTGG ATTCATTTAT GAACGAGATC AGCTGGATGT AAAATGCCCC
    CAGTGTCAGT GCAGTTTCTG CAGGAAATGC AAGAGGCCTT GGGAAGAACA GCATCGGTCT
    TTAAATTGTG ATGATTTTCA GAACTGGAAA AGGGAAAATG ATGCAGAATA CCAAGCTCAA
    GGATTGGCTG TATACCTGCT AGAGAATGGG ATCACCTGTC CCCATTGTAA ATTTTCTTAT
    GCTCTTGCAC GAGGGGGATG CATGCACTTC ATATGTTCTC AGTGTCGCCA CCAGTTCTGC
    AGTGGCTGCT ATAACACCTT TCACCAAAGA AATAAATGTA CAGTCCCGTC CTGTACGGTA
    AGGATGTCTC TCCATGCTCA CCATCCTAGA GACTGTCTCT TTTACCTCCG AGATTGGGAT
    GTCACACGTC TGCAAAGACT ACTCCAGATG TTACTGGTAC AGTTTAACAC AGATCCACCC
    GCTGGAACAC AAACTGCTCC AGGAGGTGGG TGTCGTGTGA TGGAGCAAAA GGAGATACCT
    GATGGCCTGA GAGACGAAGC TTGTGGCAAA ATAACACCTC CAGGATATGC TGGTTTGTGC
    GAGTCTCATT ATAAAGAATA TCTTGTTAGT CGTATTAATG CCCATTCTCT GGATCCATGT
    GTTCTCTATG ATCTGGCTGA GACTATGAAT GTGTGTCAGC GCTACCTGCC TGTATTGCCT
    CTACAAGATC CCACCGAGGA TGAGGCCAGC TACAGGGAGC GTCTCCTGGG GGTGTTAATA
    TCAGAGGTGC CACTGAGTAT GAACATTCCA CGGAAGCAGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818167.2
    CDS400..3504
    Translation

    Target ORF information:

    RefSeq Version XM_012962713.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962713.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGTAT CACGTCTACA CTCTGTGACC
    ACTGCTCTCA ACATCCTTGA GAAGTATGGG AGGAATCTGT TGTCCCCGCT TCGACCCAAA
    TTCTGGAGGA GTGTGAAATT TAACAACCCT GTTTTCAAGA CAACAGTAGA TGCCATTGAG
    GGTGGACGAT TTGTTCTGTC TCTTTATGGC TACTCTCAGT CACTTCCTGA TGGTCTAAGT
    TTTCCAGATT CTGTGCAGGA ACCAGATGTC TCCACTGTTG CTGCTGTAAC AGCTGATGTG
    ATTATTCTTC ACCATGAACT CAACCTACTC ATCTCGAACA TGCACCCACG GCACGAGGAA
    GCAGCACAAG AACTATTAGG TGGTGAACTG CCACAGCAGT CTCTTGTTGC TGATTACGTA
    AGTGCTCCTA AAGATCCACA ACCAGAAACC ACTTCTGCTG GCTGTTTTCT TTGTGGTACT
    CTAGTTTGTT CTGTGTTTTG TTCCTCTTGT AATGAGATGC TCTGTGAGGA GTGTGATAAG
    AGAGCACACT CTCATCCAGT CCGTGCCCAA CATGTGCGTT TACCATGTGG AAATACAGCA
    AAGCCCAGGT CATTGGATCA GGACTCCCCT TCTACAGTGT CATCTCGCCC TCCTTGGAGA
    TGTTACTCTT GCCATTTTGA AAACAAAGGG GGTTCTGTTC TGTGTGTGGT ATGTGAGCGC
    CCCCGTGGCT GTACCAGCAG CCTTTCAAAC CTATCACTTG GAAGTACTTT ACAGAGAGAC
    AGCTGGGAAT GCCAGGCTTG TACTCTGCAA AATTCTGCAT CTGCAGTTCT GTGTGCAGTG
    TGTGATCGTC CCCGATTGGC AAGCAAACCA GCCCAGGATG GTTACGATTC TGTTGCTTTG
    CTTTTGAGAG ATATACAGAG GGATAACAAG AGTATCACAT GCCCTGTGAG TGAAAAAAAT
    GGTCTATTTG CCATGAAGAA TCATCAGGAG AGTATGCATG AACCAGAAAG CATTGGTCCT
    CTGATAGATC ATACAAGGCA AGAAGAGGAG ATTGCTAAAA GTGAGGGCTG GCAGTGTTCC
    CATTGCACTT TCTTTAACAC ACGAAATGGA CGTGTTTGTG AGATTTGTGA CAGGACCAGT
    GAGGGGACAG CAGCAACTGA ACAACCCAAG GCACTTGATA TGGCACATGT GCAGGAGGAG
    ATCCGGCAGA AGCGACTAAG AGAGGATGGA GCTAAAATAG TGGCATTGAT CCGGGAAGGA
    GAGAAGTATG GGGTTCTACC TGAAGAGGTG TGTAGCGCAA TACGATATTC TGGTACAGAA
    GTGCCTCAGT GGTGGCTGCA GACTGAACTT CCCTATGTAT TAGAGCGTCT GCTGGATGCT
    GCTTCACAGA AAGCAGGAGA TACAATTGGT GCCCTAACAG TGGAAGAAGC TCGTGATGCT
    TGGGTGTCCA GTGGTGGAGA CATGGATGCT GCTGTCATCT TATGTGTGAC AGAGCGAAGA
    AGGAAGGTTG ATGCACTCTC CGGCCTAGGT TTTCCAGAAA GGCATAAAGT GGTAGCTGCT
    CTTTATGAAA GTGCTGGAGA TGTTGGTAGA GCACTAAGTA TATTACAGAG ACCACTTCTG
    GAGCCTTTTC TTATACGCAT GTGGGAAGAA CCACAACCAC CCCTTCGGCT AGACTCGGCA
    GATAGACAGG CAGTTCTCCG TAGGTTACTT GCAGAACATT CCTTGCACAG TTGGGGTCGG
    GCAGAGCTAG CTTTGTCATT GCTTATTGAA GGAGAAGGGC GCTATGAACT CCAGGATGTT
    GTAGAGGCAG TGCGGGAATC GCAGGACAGA GACTTTATCA AAAGGATGTT AACACAGGAG
    TGTGCAGTGT GTGGATGGGA GCTCCCCCGT AACAAGATGC GTTCTCTAAC CTCATGTGAG
    TGTTGTATCT GTCCTGATTG CTTCCGGATG CATTTCACTG TAGCAGTGAA AGAGAAACAT
    ATTAGAGACA TGATATGTCC AGCTTGTGAG GAGCCAGAAA TAAGCGACGA GGGGGAGCTT
    CTTCACTATT TCTCTACTCT GGATATCCTG TTACGGGACA CTTTGGAGGT TGATGCATAT
    GATTTGTTCC ATAAAAAACT AACTGAAAGA ACACTTATGA AGGACCCAAA GTTCCTGTGG
    TGTACACATT GTTCCTTTGG ATTCATTTAT GAACGAGATC AGCTGGATGT AAAATGCCCC
    CAGTGTCAGT GCAGTTTCTG CAGGAAATGC AAGAGGCCTT GGGAAGAACA GCATCGGTCT
    TTAAATTGTG ATGATTTTCA GAACTGGAAA AGGGAAAATG ATGCAGAATA CCAAGCTCAA
    GGATTGGCTG TATACCTGCT AGAGAATGGG ATCACCTGTC CCCATTGTAA ATTTTCTTAT
    GCTCTTGCAC GAGGGGGATG CATGCACTTC ATATGTTCTC AGTGTCGCCA CCAGTTCTGC
    AGTGGCTGCT ATAACACCTT TCACCAAAGA AATAAATGTA CAGTCCCGTC CTGTACGGTA
    AGGATGTCTC TCCATGCTCA CCATCCTAGA GACTGTCTCT TTTACCTCCG AGATTGGGAT
    GTCACACGTC TGCAAAGACT ACTCCAGATG TTACTGGTAC AGTTTAACAC AGATCCACCC
    GCTGGAACAC AAACTGCTCC AGGAGGTGGG TGTCGTGTGA TGGAGCAAAA GGAGATACCT
    GATGGCCTGA GAGACGAAGC TTGTGGCAAA ATAACACCTC CAGGATATGC TGGTTTGTGC
    GAGTCTCATT ATAAAGAATA TCTTGTTAGT CGTATTAATG CCCATTCTCT GGATCCATGT
    GTTCTCTATG ATCTGGCTGA GACTATGAAT GTGTGTCAGC GCTACCTGCC TGTATTGCCT
    CTACAAGATC CCACCGAGGA TGAGGCCAGC TACAGGGAGC GTCTCCTGGG GGTGTTAATA
    TCAGAGGTGC CACTGAGTAT GAACATTCCA CGGAAGCAGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29815
    Clone ID Related Accession (Same CDS sequence) XM_012962713.2 , XM_012962335.2
    Accession Version XM_012962713.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase RNF31 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGTAT CACGTCTACA CTCTGTGACC
    ACTGCTCTCA ACATCCTTGA GAAGTATGGG AGGAATCTGT TGTCCCCGCT TCGACCCAAA
    TTCTGGAGGA GTGTGAAATT TAACAACCCT GTTTTCAAGA CAACAGTAGA TGCCATTGAG
    GGTGGACGAT TTGTTCTGTC TCTTTATGGC TACTCTCAGT CACTTCCTGA TGGTCTAAGT
    TTTCCAGATT CTGTGCAGGA ACCAGATGTC TCCACTGTTG CTGCTGTAAC AGCTGATGTG
    ATTATTCTTC ACCATGAACT CAACCTACTC ATCTCGAACA TGCACCCACG GCACGAGGAA
    GCAGCACAAG AACTATTAGG TGGTGAACTG CCACAGCAGT CTCTTGTTGC TGATTACGTA
    AGTGCTCCTA AAGATCTACA ACCAGAAACC ACTTCTGCTG GCTGTTTTCT TTGTGGTACT
    CTAGTTTGTT CTGTGTTTTG TTCCTCTTGT AATGAGATGC TCTGTGAGGA GTGTGATAAG
    AGAGCACACT CTCATCCAGT CCGTGCCCAA CATGTGCGTT TACCATGTGG AAATACAGCA
    AAGCCCAGGT CATTGGATCA GGACTCCCCT TCTACAGTGT CATCTCGCCC TCCTTGGAGA
    TGTTACTCTT GCCATTTTGA AAACAAAGGG GGTTCTGTTC TGTGTGTGGT ATGTGAGCGC
    CCCCGTGGCT GTACCAGCAG CCTTTCAAAC CTATCACTTG GAAGTACTTT ACAGAGAGAC
    AGCTGGGAAT GCCAGGCTTG TACTCTGCAA AATTCTGCAT CTGCAGTTCT GTGTGCAGTG
    TGTGATCGTC CCCGATTGGC AAGCAAACCA GCCCAGGATG GTTACGATTC TGTTGCTTTG
    CTTTTGAGAG ATATACAGAG GGATAACAAG AGTATCACAT GCCCTGTGAG TGAAAAAAAT
    GGTCTATTTG CCATGAAGAA TCATCAGGAG AGTATGCATG AACCAGAAAG CATTGGTCCT
    CTGATAGATC ATACAAGGCA AGAAGAGGAG ATTGCTAAAA GTGAGGGCTG GCAGTGTTCC
    CATTGCACTT TCTTTAACAC ACGAAATGGA CGTGTTTGTG AGATTTGTGA CAGGACCAGT
    GAGGGGACAG CAGCAACTGA ACAACCCAAG GCACTTGATA TGGCACATGT GCAGGAGGAG
    ATCCGGCAGA AGCGACTAAG AGAGGATGGA GCTAAAATAG TGGCATTGAT CCGGGAAGGA
    GAGAAGTATG GGGTTCTACC TGAAGAGGTG TGTAGCGCAA TACGATATTC TGGTACAGAA
    GTGCCTCAGT GGTGGCTGCA GACTGAACTT CCCTATGTAT TAGAGCGTCT GCTGGATGCT
    GCTTCACAGA AAGCAGGAGA TACAATTGGT GCCCTAACAG TGGAAGAAGC TCGTGATGCT
    TGGGTGTCCA GTGGTGGAGA CATGGATGCT GCTGTCATCT TATGTGTGAC AGAGCGAAGA
    AGGAAGGTTG ATGCACTCTC CGGCCTAGGT TTTCCAGAAA GGCATAAAGT GGTAGCTGCT
    CTTTATGAAA GTGCTGGAGA TGTTGGTAGA GCACTAAGTA TATTACAGAG ACCACTTCTG
    GAGCCTTTTC TTATACGCAT GTGGGAAGAA CCACAACCAC CCCTTCGGCT AGACTCAGCA
    GATAGACAGG CAGTTCTCCG TAGGTTACTT GCAGAACATT CCTTGCACAG TTGGGGTCGG
    GCAGAGCTAG CTTTGTCATT GCTTATTGAA GGAGAAGGGC GCTATGAACT CCAGGATGTT
    GTAGAGGCAG TGCGGGAATC GCAGGACAGA GACTTTATCA AAAGGATGTT AACACAGGAG
    TGTGCAGTGT GTGGATGGGA GCTCCCCCGT AACAAGATGC GTTCTCTAAC CTCATGTGAG
    TGTTGTATCT GTCCTGATTG CTTCCGGATG CATTTCACTG TAGCAGTGAA AGAGAAACAT
    ATTAGAGACA TGATATGTCC AGCTTGTGAG GAGCCAGAAA TAAGCGACGA GGGGGAGCTT
    CTTCACTATT TCTCTACTCT GGATATCCTG TTACGGGACA CTTTGGAGGT TGATGCATAT
    GATTTGTTCC ATAAAAAACT AACTGAAAGA ACACTTATGA AGGACCCAAA GTTCCTGTGG
    TGTACACATT GTTCCTTTGG ATTCATTTAT GAACGAGATC AGCTGGATGT AAAATGCCCC
    CAGTGTCAGT GCAGTTTCTG CAGGAAATGC AAGAGGCCTT GGGAAGAACA GCATCGGTCT
    TTAAATTGTG ATGATTTTCA GAACTGGAAA AGGGAAAATG ATGCAGAATA CCAAGCTCAA
    GGATTGGCTG TATACCTGCT AGAGAATGGG ATCACCTGTC CCCATTGTAA ATTTTCTTAT
    GCTCTTGCAC GAGGGGGATG CATGCACTTC ATATGTTCTC AGTGTCGCCA CCAGTTCTGC
    AGTGGCTGCT ATAACACCTT TCACCAAAGA AATAAATGTA CAGTCCCGTC CTGTACGGTA
    AGGATGTCTC TCCATGCTCA CCATCCTAGA GACTGTCTCT TTTACCTCCG AGATTGGGAT
    GTCACACGTC TGCAAAGACT ACTCCAGATG TTACTGGTAC AGTTTAACAC AGATCCACCC
    GCTGGAACAC AAACTGCTCC AGGAGGTGGG TGTCGTGTGA TGGAGCAAAA GGAGATACCT
    GATGGCCTGA GAGACGAAGC TTGTGGCAAA ATAACACCTC CAGGATATGC TGGTTTGTGC
    GAGTCTCATT ATAAAGAATA TCTTGTTAGT CGTATTAATG CCCATTCTCT GGATCCATGT
    GTTCTCTATG ATCTGGCTGA GACTATGAAT GTGTGTCAGC GCTACCTGCC TGTATTGCCT
    CTACAAGATC CCACCGAGAA TGAGGCCAGC TACAGGGAGC GTCTCCTGGG GGTGTTAATA
    TCAGAGGTGC CACTGAGTAT GAACATTCCA CGGAAGCAGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818167.1
    CDS392..3496
    Translation

    Target ORF information:

    RefSeq Version XM_012962713.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962713.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGTAT CACGTCTACA CTCTGTGACC
    ACTGCTCTCA ACATCCTTGA GAAGTATGGG AGGAATCTGT TGTCCCCGCT TCGACCCAAA
    TTCTGGAGGA GTGTGAAATT TAACAACCCT GTTTTCAAGA CAACAGTAGA TGCCATTGAG
    GGTGGACGAT TTGTTCTGTC TCTTTATGGC TACTCTCAGT CACTTCCTGA TGGTCTAAGT
    TTTCCAGATT CTGTGCAGGA ACCAGATGTC TCCACTGTTG CTGCTGTAAC AGCTGATGTG
    ATTATTCTTC ACCATGAACT CAACCTACTC ATCTCGAACA TGCACCCACG GCACGAGGAA
    GCAGCACAAG AACTATTAGG TGGTGAACTG CCACAGCAGT CTCTTGTTGC TGATTACGTA
    AGTGCTCCTA AAGATCTACA ACCAGAAACC ACTTCTGCTG GCTGTTTTCT TTGTGGTACT
    CTAGTTTGTT CTGTGTTTTG TTCCTCTTGT AATGAGATGC TCTGTGAGGA GTGTGATAAG
    AGAGCACACT CTCATCCAGT CCGTGCCCAA CATGTGCGTT TACCATGTGG AAATACAGCA
    AAGCCCAGGT CATTGGATCA GGACTCCCCT TCTACAGTGT CATCTCGCCC TCCTTGGAGA
    TGTTACTCTT GCCATTTTGA AAACAAAGGG GGTTCTGTTC TGTGTGTGGT ATGTGAGCGC
    CCCCGTGGCT GTACCAGCAG CCTTTCAAAC CTATCACTTG GAAGTACTTT ACAGAGAGAC
    AGCTGGGAAT GCCAGGCTTG TACTCTGCAA AATTCTGCAT CTGCAGTTCT GTGTGCAGTG
    TGTGATCGTC CCCGATTGGC AAGCAAACCA GCCCAGGATG GTTACGATTC TGTTGCTTTG
    CTTTTGAGAG ATATACAGAG GGATAACAAG AGTATCACAT GCCCTGTGAG TGAAAAAAAT
    GGTCTATTTG CCATGAAGAA TCATCAGGAG AGTATGCATG AACCAGAAAG CATTGGTCCT
    CTGATAGATC ATACAAGGCA AGAAGAGGAG ATTGCTAAAA GTGAGGGCTG GCAGTGTTCC
    CATTGCACTT TCTTTAACAC ACGAAATGGA CGTGTTTGTG AGATTTGTGA CAGGACCAGT
    GAGGGGACAG CAGCAACTGA ACAACCCAAG GCACTTGATA TGGCACATGT GCAGGAGGAG
    ATCCGGCAGA AGCGACTAAG AGAGGATGGA GCTAAAATAG TGGCATTGAT CCGGGAAGGA
    GAGAAGTATG GGGTTCTACC TGAAGAGGTG TGTAGCGCAA TACGATATTC TGGTACAGAA
    GTGCCTCAGT GGTGGCTGCA GACTGAACTT CCCTATGTAT TAGAGCGTCT GCTGGATGCT
    GCTTCACAGA AAGCAGGAGA TACAATTGGT GCCCTAACAG TGGAAGAAGC TCGTGATGCT
    TGGGTGTCCA GTGGTGGAGA CATGGATGCT GCTGTCATCT TATGTGTGAC AGAGCGAAGA
    AGGAAGGTTG ATGCACTCTC CGGCCTAGGT TTTCCAGAAA GGCATAAAGT GGTAGCTGCT
    CTTTATGAAA GTGCTGGAGA TGTTGGTAGA GCACTAAGTA TATTACAGAG ACCACTTCTG
    GAGCCTTTTC TTATACGCAT GTGGGAAGAA CCACAACCAC CCCTTCGGCT AGACTCAGCA
    GATAGACAGG CAGTTCTCCG TAGGTTACTT GCAGAACATT CCTTGCACAG TTGGGGTCGG
    GCAGAGCTAG CTTTGTCATT GCTTATTGAA GGAGAAGGGC GCTATGAACT CCAGGATGTT
    GTAGAGGCAG TGCGGGAATC GCAGGACAGA GACTTTATCA AAAGGATGTT AACACAGGAG
    TGTGCAGTGT GTGGATGGGA GCTCCCCCGT AACAAGATGC GTTCTCTAAC CTCATGTGAG
    TGTTGTATCT GTCCTGATTG CTTCCGGATG CATTTCACTG TAGCAGTGAA AGAGAAACAT
    ATTAGAGACA TGATATGTCC AGCTTGTGAG GAGCCAGAAA TAAGCGACGA GGGGGAGCTT
    CTTCACTATT TCTCTACTCT GGATATCCTG TTACGGGACA CTTTGGAGGT TGATGCATAT
    GATTTGTTCC ATAAAAAACT AACTGAAAGA ACACTTATGA AGGACCCAAA GTTCCTGTGG
    TGTACACATT GTTCCTTTGG ATTCATTTAT GAACGAGATC AGCTGGATGT AAAATGCCCC
    CAGTGTCAGT GCAGTTTCTG CAGGAAATGC AAGAGGCCTT GGGAAGAACA GCATCGGTCT
    TTAAATTGTG ATGATTTTCA GAACTGGAAA AGGGAAAATG ATGCAGAATA CCAAGCTCAA
    GGATTGGCTG TATACCTGCT AGAGAATGGG ATCACCTGTC CCCATTGTAA ATTTTCTTAT
    GCTCTTGCAC GAGGGGGATG CATGCACTTC ATATGTTCTC AGTGTCGCCA CCAGTTCTGC
    AGTGGCTGCT ATAACACCTT TCACCAAAGA AATAAATGTA CAGTCCCGTC CTGTACGGTA
    AGGATGTCTC TCCATGCTCA CCATCCTAGA GACTGTCTCT TTTACCTCCG AGATTGGGAT
    GTCACACGTC TGCAAAGACT ACTCCAGATG TTACTGGTAC AGTTTAACAC AGATCCACCC
    GCTGGAACAC AAACTGCTCC AGGAGGTGGG TGTCGTGTGA TGGAGCAAAA GGAGATACCT
    GATGGCCTGA GAGACGAAGC TTGTGGCAAA ATAACACCTC CAGGATATGC TGGTTTGTGC
    GAGTCTCATT ATAAAGAATA TCTTGTTAGT CGTATTAATG CCCATTCTCT GGATCCATGT
    GTTCTCTATG ATCTGGCTGA GACTATGAAT GTGTGTCAGC GCTACCTGCC TGTATTGCCT
    CTACAAGATC CCACCGAGAA TGAGGCCAGC TACAGGGAGC GTCTCCTGGG GGTGTTAATA
    TCAGAGGTGC CACTGAGTAT GAACATTCCA CGGAAGCAGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29815
    Clone ID Related Accession (Same CDS sequence) XM_012962713.2 , XM_012962335.2
    Accession Version XM_012962335.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase RNF31 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962335.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGTAT CACGTCTACA CTCTGTGACC
    ACTGCTCTCA ACATCCTTGA GAAGTATGGG AGGAATCTGT TGTCCCCGCT TCGACCCAAA
    TTCTGGAGGA GTGTGAAATT TAACAACCCT GTTTTCAAGA CAACAGTAGA TGCCATTGAG
    GGTGGACGAT TTGTTCTGTC TCTTTATGGC TACTCTCAGT CACTTCCTGA TGGTCTAAGT
    TTTCCAGATT CTGTGCAGGA ACCAGATGTC TCCACTGTTG CTGCTGTAAC AGCTGATGTG
    ATTATTCTTC ACCATGAACT CAACCTACTC ATCTCGAACA TGCACCCACG GCACGAGGAA
    GCAGCACAAG AACTATTAGG TGGTGAACTG CCACAGCAGT CTCTTGTTGC TGATTACGTA
    AGTGCTCCTA AAGATCTACA ACCAGAAACC ACTTCTGCTG GCTGTTTTCT TTGTGGTACT
    CTAGTTTGTT CTGTGTTTTG TTCCTCTTGT AATGAGATGC TCTGTGAGGA GTGTGATAAG
    AGAGCACACT CTCATCCAGT CCGTGCCCAA CATGTGCGTT TACCATGTGG AAATACAGCA
    AAGCCCAGGT CATTGGATCA GGACTCCCCT TCTACAGTGT CATCTCGCCC TCCTTGGAGA
    TGTTACTCTT GCCATTTTGA AAACAAAGGG GGTTCTGTTC TGTGTGTGGT ATGTGAGCGC
    CCCCGTGGCT GTACCAGCAG CCTTTCAAAC CTATCACTTG GAAGTACTTT ACAGAGAGAC
    AGCTGGGAAT GCCAGGCTTG TACTCTGCAA AATTCTGCAT CTGCAGTTCT GTGTGCAGTG
    TGTGATCGTC CCCGATTGGC AAGCAAACCA GCCCAGGATG GTTACGATTC TGTTGCTTTG
    CTTTTGAGAG ATATACAGAG GGATAACAAG AGTATCACAT GCCCTGTGAG TGAAAAAAAT
    GGTCTATTTG CCATGAAGAA TCATCAGGAG AGTATGCATG AACCAGAAAG CATTGGTCCT
    CTGATAGATC ATACAAGGCA AGAAGAGGAG ATTGCTAAAA GTGAGGGCTG GCAGTGTTCC
    CATTGCACTT TCTTTAACAC ACGAAATGGA CGTGTTTGTG AGATTTGTGA CAGGACCAGT
    GAGGGGACAG CAGCAACTGA ACAACCCAAG GCACTTGATA TGGCACATGT GCAGGAGGAG
    ATCCGGCAGA AGCGACTAAG AGAGGATGGA GCTAAAATAG TGGCATTGAT CCGGGAAGGA
    GAGAAGTATG GGGTTCTACC TGAAGAGGTG TGTAGCGCAA TACGATATTC TGGTACAGAA
    GTGCCTCAGT GGTGGCTGCA GACTGAACTT CCCTATGTAT TAGAGCGTCT GCTGGATGCT
    GCTTCACAGA AAGCAGGAGA TACAATTGGT GCCCTAACAG TGGAAGAAGC TCGTGATGCT
    TGGGTGTCCA GTGGTGGAGA CATGGATGCT GCTGTCATCT TATGTGTGAC AGAGCGAAGA
    AGGAAGGTTG ATGCACTCTC CGGCCTAGGT TTTCCAGAAA GGCATAAAGT GGTAGCTGCT
    CTTTATGAAA GTGCTGGAGA TGTTGGTAGA GCACTAAGTA TATTACAGAG ACCACTTCTG
    GAGCCTTTTC TTATACGCAT GTGGGAAGAA CCACAACCAC CCCTTCGGCT AGACTCAGCA
    GATAGACAGG CAGTTCTCCG TAGGTTACTT GCAGAACATT CCTTGCACAG TTGGGGTCGG
    GCAGAGCTAG CTTTGTCATT GCTTATTGAA GGAGAAGGGC GCTATGAACT CCAGGATGTT
    GTAGAGGCAG TGCGGGAATC GCAGGACAGA GACTTTATCA AAAGGATGTT AACACAGGAG
    TGTGCAGTGT GTGGATGGGA GCTCCCCCGT AACAAGATGC GTTCTCTAAC CTCATGTGAG
    TGTTGTATCT GTCCTGATTG CTTCCGGATG CATTTCACTG TAGCAGTGAA AGAGAAACAT
    ATTAGAGACA TGATATGTCC AGCTTGTGAG GAGCCAGAAA TAAGCGACGA GGGGGAGCTT
    CTTCACTATT TCTCTACTCT GGATATCCTG TTACGGGACA CTTTGGAGGT TGATGCATAT
    GATTTGTTCC ATAAAAAACT AACTGAAAGA ACACTTATGA AGGACCCAAA GTTCCTGTGG
    TGTACACATT GTTCCTTTGG ATTCATTTAT GAACGAGATC AGCTGGATGT AAAATGCCCC
    CAGTGTCAGT GCAGTTTCTG CAGGAAATGC AAGAGGCCTT GGGAAGAACA GCATCGGTCT
    TTAAATTGTG ATGATTTTCA GAACTGGAAA AGGGAAAATG ATGCAGAATA CCAAGCTCAA
    GGATTGGCTG TATACCTGCT AGAGAATGGG ATCACCTGTC CCCATTGTAA ATTTTCTTAT
    GCTCTTGCAC GAGGGGGATG CATGCACTTC ATATGTTCTC AGTGTCGCCA CCAGTTCTGC
    AGTGGCTGCT ATAACACCTT TCACCAAAGA AATAAATGTA CAGTCCCGTC CTGTACGGTA
    AGGATGTCTC TCCATGCTCA CCATCCTAGA GACTGTCTCT TTTACCTCCG AGATTGGGAT
    GTCACACGTC TGCAAAGACT ACTCCAGATG TTACTGGTAC AGTTTAACAC AGATCCACCC
    GCTGGAACAC AAACTGCTCC AGGAGGTGGG TGTCGTGTGA TGGAGCAAAA GGAGATACCT
    GATGGCCTGA GAGACGAAGC TTGTGGCAAA ATAACACCTC CAGGATATGC TGGTTTGTGC
    GAGTCTCATT ATAAAGAATA TCTTGTTAGT CGTATTAATG CCCATTCTCT GGATCCATGT
    GTTCTCTATG ATCTGGCTGA GACTATGAAT GTGTGTCAGC GCTACCTGCC TGTATTGCCT
    CTACAAGATC CCACCGAGAA TGAGGCCAGC TACAGGGAGC GTCTCCTGGG GGTGTTAATA
    TCAGAGGTGC CACTGAGTAT GAACATTCCA CGGAAGCAGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817789.1
    CDS373..3477
    Translation

    Target ORF information:

    RefSeq Version XM_012962335.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962335.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGTAT CACGTCTACA CTCTGTGACC
    ACTGCTCTCA ACATCCTTGA GAAGTATGGG AGGAATCTGT TGTCCCCGCT TCGACCCAAA
    TTCTGGAGGA GTGTGAAATT TAACAACCCT GTTTTCAAGA CAACAGTAGA TGCCATTGAG
    GGTGGACGAT TTGTTCTGTC TCTTTATGGC TACTCTCAGT CACTTCCTGA TGGTCTAAGT
    TTTCCAGATT CTGTGCAGGA ACCAGATGTC TCCACTGTTG CTGCTGTAAC AGCTGATGTG
    ATTATTCTTC ACCATGAACT CAACCTACTC ATCTCGAACA TGCACCCACG GCACGAGGAA
    GCAGCACAAG AACTATTAGG TGGTGAACTG CCACAGCAGT CTCTTGTTGC TGATTACGTA
    AGTGCTCCTA AAGATCTACA ACCAGAAACC ACTTCTGCTG GCTGTTTTCT TTGTGGTACT
    CTAGTTTGTT CTGTGTTTTG TTCCTCTTGT AATGAGATGC TCTGTGAGGA GTGTGATAAG
    AGAGCACACT CTCATCCAGT CCGTGCCCAA CATGTGCGTT TACCATGTGG AAATACAGCA
    AAGCCCAGGT CATTGGATCA GGACTCCCCT TCTACAGTGT CATCTCGCCC TCCTTGGAGA
    TGTTACTCTT GCCATTTTGA AAACAAAGGG GGTTCTGTTC TGTGTGTGGT ATGTGAGCGC
    CCCCGTGGCT GTACCAGCAG CCTTTCAAAC CTATCACTTG GAAGTACTTT ACAGAGAGAC
    AGCTGGGAAT GCCAGGCTTG TACTCTGCAA AATTCTGCAT CTGCAGTTCT GTGTGCAGTG
    TGTGATCGTC CCCGATTGGC AAGCAAACCA GCCCAGGATG GTTACGATTC TGTTGCTTTG
    CTTTTGAGAG ATATACAGAG GGATAACAAG AGTATCACAT GCCCTGTGAG TGAAAAAAAT
    GGTCTATTTG CCATGAAGAA TCATCAGGAG AGTATGCATG AACCAGAAAG CATTGGTCCT
    CTGATAGATC ATACAAGGCA AGAAGAGGAG ATTGCTAAAA GTGAGGGCTG GCAGTGTTCC
    CATTGCACTT TCTTTAACAC ACGAAATGGA CGTGTTTGTG AGATTTGTGA CAGGACCAGT
    GAGGGGACAG CAGCAACTGA ACAACCCAAG GCACTTGATA TGGCACATGT GCAGGAGGAG
    ATCCGGCAGA AGCGACTAAG AGAGGATGGA GCTAAAATAG TGGCATTGAT CCGGGAAGGA
    GAGAAGTATG GGGTTCTACC TGAAGAGGTG TGTAGCGCAA TACGATATTC TGGTACAGAA
    GTGCCTCAGT GGTGGCTGCA GACTGAACTT CCCTATGTAT TAGAGCGTCT GCTGGATGCT
    GCTTCACAGA AAGCAGGAGA TACAATTGGT GCCCTAACAG TGGAAGAAGC TCGTGATGCT
    TGGGTGTCCA GTGGTGGAGA CATGGATGCT GCTGTCATCT TATGTGTGAC AGAGCGAAGA
    AGGAAGGTTG ATGCACTCTC CGGCCTAGGT TTTCCAGAAA GGCATAAAGT GGTAGCTGCT
    CTTTATGAAA GTGCTGGAGA TGTTGGTAGA GCACTAAGTA TATTACAGAG ACCACTTCTG
    GAGCCTTTTC TTATACGCAT GTGGGAAGAA CCACAACCAC CCCTTCGGCT AGACTCAGCA
    GATAGACAGG CAGTTCTCCG TAGGTTACTT GCAGAACATT CCTTGCACAG TTGGGGTCGG
    GCAGAGCTAG CTTTGTCATT GCTTATTGAA GGAGAAGGGC GCTATGAACT CCAGGATGTT
    GTAGAGGCAG TGCGGGAATC GCAGGACAGA GACTTTATCA AAAGGATGTT AACACAGGAG
    TGTGCAGTGT GTGGATGGGA GCTCCCCCGT AACAAGATGC GTTCTCTAAC CTCATGTGAG
    TGTTGTATCT GTCCTGATTG CTTCCGGATG CATTTCACTG TAGCAGTGAA AGAGAAACAT
    ATTAGAGACA TGATATGTCC AGCTTGTGAG GAGCCAGAAA TAAGCGACGA GGGGGAGCTT
    CTTCACTATT TCTCTACTCT GGATATCCTG TTACGGGACA CTTTGGAGGT TGATGCATAT
    GATTTGTTCC ATAAAAAACT AACTGAAAGA ACACTTATGA AGGACCCAAA GTTCCTGTGG
    TGTACACATT GTTCCTTTGG ATTCATTTAT GAACGAGATC AGCTGGATGT AAAATGCCCC
    CAGTGTCAGT GCAGTTTCTG CAGGAAATGC AAGAGGCCTT GGGAAGAACA GCATCGGTCT
    TTAAATTGTG ATGATTTTCA GAACTGGAAA AGGGAAAATG ATGCAGAATA CCAAGCTCAA
    GGATTGGCTG TATACCTGCT AGAGAATGGG ATCACCTGTC CCCATTGTAA ATTTTCTTAT
    GCTCTTGCAC GAGGGGGATG CATGCACTTC ATATGTTCTC AGTGTCGCCA CCAGTTCTGC
    AGTGGCTGCT ATAACACCTT TCACCAAAGA AATAAATGTA CAGTCCCGTC CTGTACGGTA
    AGGATGTCTC TCCATGCTCA CCATCCTAGA GACTGTCTCT TTTACCTCCG AGATTGGGAT
    GTCACACGTC TGCAAAGACT ACTCCAGATG TTACTGGTAC AGTTTAACAC AGATCCACCC
    GCTGGAACAC AAACTGCTCC AGGAGGTGGG TGTCGTGTGA TGGAGCAAAA GGAGATACCT
    GATGGCCTGA GAGACGAAGC TTGTGGCAAA ATAACACCTC CAGGATATGC TGGTTTGTGC
    GAGTCTCATT ATAAAGAATA TCTTGTTAGT CGTATTAATG CCCATTCTCT GGATCCATGT
    GTTCTCTATG ATCTGGCTGA GACTATGAAT GTGTGTCAGC GCTACCTGCC TGTATTGCCT
    CTACAAGATC CCACCGAGAA TGAGGCCAGC TACAGGGAGC GTCTCCTGGG GGTGTTAATA
    TCAGAGGTGC CACTGAGTAT GAACATTCCA CGGAAGCAGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29816
    Clone ID Related Accession (Same CDS sequence) XM_018092848.1
    Accession Version XM_018092848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase RNF31 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGTAT CACGTCTACA CTCTGTGACC
    ACTGCTCTCA ACATCCTTGA GAAGTATGGG AGGAATCTGT TGTCCCCGCT TCGACCCAAA
    TTCTGGAGGA GTGTGAAATT TAACAACCCT GTTTTCAAGA CAACAGTAGA TGCCATTGAG
    GGTGGACGAT TTGTTCTGTC TCTTTATGGC TACTCTCAGT CACTTCCTGA TGGTCTAAGT
    TTTCCAGATT CTGTGCAGGA ACCAGATGTC TCCACTGTTG CTGCTGTAAC AGCTGATGTG
    ATTATTCTTC ACCATGAACT CAACCTACTC ATCTCGAACA TGCACCCACG GCACGAGGAA
    GCAGCACAAG AACTATTAGG TGGTGAACTG CCACAGTCTC TTGTTGCTGA TTACGTAAGT
    GCTCCTAAAG ATCTACAACC AGAAACCACT TCTGCTGGCT GTTTTCTTTG TGGTACTCTA
    GTTTGTTCTG TGTTTTGTTC CTCTTGTAAT GAGATGCTCT GTGAGGAGTG TGATAAGAGA
    GCACACTCTC ATCCAGTCCG TGCCCAACAT GTGCGTTTAC CATGTGGAAA TACAGCAAAG
    CCCAGGTCAT TGGATCAGGA CTCCCCTTCT ACAGTGTCAT CTCGCCCTCC TTGGAGATGT
    TACTCTTGCC ATTTTGAAAA CAAAGGGGGT TCTGTTCTGT GTGTGGTATG TGAGCGCCCC
    CGTGGCTGTA CCAGCAGCCT TTCAAACCTA TCACTTGGAA GTACTTTACA GAGAGACAGC
    TGGGAATGCC AGGCTTGTAC TCTGCAAAAT TCTGCATCTG CAGTTCTGTG TGCAGTGTGT
    GATCGTCCCC GATTGGCAAG CAAACCAGCC CAGGATGGTT ACGATTCTGT TGCTTTGCTT
    TTGAGAGATA TACAGAGGGA TAACAAGAGT ATCACATGCC CTGTGAGTGA AAAAAATGGT
    CTATTTGCCA TGAAGAATCA TCAGGAGAGT ATGCATGAAC CAGAAAGCAT TGGTCCTCTG
    ATAGATCATA CAAGGCAAGA AGAGGAGATT GCTAAAAGTG AGGGCTGGCA GTGTTCCCAT
    TGCACTTTCT TTAACACACG AAATGGACGT GTTTGTGAGA TTTGTGACAG GACCAGTGAG
    GGGACAGCAG CAACTGAACA ACCCAAGGCA CTTGATATGG CACATGTGCA GGAGGAGATC
    CGGCAGAAGC GACTAAGAGA GGATGGAGCT AAAATAGTGG CATTGATCCG GGAAGGAGAG
    AAGTATGGGG TTCTACCTGA AGAGGTGTGT AGCGCAATAC GATATTCTGG TACAGAAGTG
    CCTCAGTGGT GGCTGCAGAC TGAACTTCCC TATGTATTAG AGCGTCTGCT GGATGCTGCT
    TCACAGAAAG CAGGAGATAC AATTGGTGCC CTAACAGTGG AAGAAGCTCG TGATGCTTGG
    GTGTCCAGTG GTGGAGACAT GGATGCTGCT GTCATCTTAT GTGTGACAGA GCGAAGAAGG
    AAGGTTGATG CACTCTCCGG CCTAGGTTTT CCAGAAAGGC ATAAAGTGGT AGCTGCTCTT
    TATGAAAGTG CTGGAGATGT TGGTAGAGCA CTAAGTATAT TACAGAGACC ACTTCTGGAG
    CCTTTTCTTA TACGCATGTG GGAAGAACCA CAACCACCCC TTCGGCTAGA CTCAGCAGAT
    AGACAGGCAG TTCTCCGTAG GTTACTTGCA GAACATTCCT TGCACAGTTG GGGTCGGGCA
    GAGCTAGCTT TGTCATTGCT TATTGAAGGA GAAGGGCGCT ATGAACTCCA GGATGTTGTA
    GAGGCAGTGC GGGAATCGCA GGACAGAGAC TTTATCAAAA GGATGTTAAC ACAGGAGTGT
    GCAGTGTGTG GATGGGAGCT CCCCCGTAAC AAGATGCGTT CTCTAACCTC ATGTGAGTGT
    TGTATCTGTC CTGATTGCTT CCGGATGCAT TTCACTGTAG CAGTGAAAGA GAAACATATT
    AGAGACATGA TATGTCCAGC TTGTGAGGAG CCAGAAATAA GCGACGAGGG GGAGCTTCTT
    CACTATTTCT CTACTCTGGA TATCCTGTTA CGGGACACTT TGGAGGTTGA TGCATATGAT
    TTGTTCCATA AAAAACTAAC TGAAAGAACA CTTATGAAGG ACCCAAAGTT CCTGTGGTGT
    ACACATTGTT CCTTTGGATT CATTTATGAA CGAGATCAGC TGGATGTAAA ATGCCCCCAG
    TGTCAGTGCA GTTTCTGCAG GAAATGCAAG AGGCCTTGGG AAGAACAGCA TCGGTCTTTA
    AATTGTGATG ATTTTCAGAA CTGGAAAAGG GAAAATGATG CAGAATACCA AGCTCAAGGA
    TTGGCTGTAT ACCTGCTAGA GAATGGGATC ACCTGTCCCC ATTGTAAATT TTCTTATGCT
    CTTGCACGAG GGGGATGCAT GCACTTCATA TGTTCTCAGT GTCGCCACCA GTTCTGCAGT
    GGCTGCTATA ACACCTTTCA CCAAAGAAAT AAATGTACAG TCCCGTCCTG TACGGTAAGG
    ATGTCTCTCC ATGCTCACCA TCCTAGAGAC TGTCTCTTTT ACCTCCGAGA TTGGGATGTC
    ACACGTCTGC AAAGACTACT CCAGATGTTA CTGGTACAGT TTAACACAGA TCCACCCGCT
    GGAACACAAA CTGCTCCAGG AGGTGGGTGT CGTGTGATGG AGCAAAAGGA GATACCTGAT
    GGCCTGAGAG ACGAAGCTTG TGGCAAAATA ACACCTCCAG GATATGCTGG TTTGTGCGAG
    TCTCATTATA AAGAATATCT TGTTAGTCGT ATTAATGCCC ATTCTCTGGA TCCATGTGTT
    CTCTATGATC TGGCTGAGAC TATGAATGTG TGTCAGCGCT ACCTGCCTGT ATTGCCTCTA
    CAAGATCCCA CCGAGAATGA GGCCAGCTAC AGGGAGCGTC TCCTGGGGGT GTTAATATCA
    GAGGTGCCAC TGAGTATGAA CATTCCACGG AAGCAGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948337.1
    CDS392..3493
    Translation

    Target ORF information:

    RefSeq Version XM_018092848.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger protein 31 (rnf31), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAAC ATCCTATAAG GAGTGCTCTG GAAAATGCAT TAATTAATCA ACCGGGGGCT 
    GTGACTCCTG AATTCCTGAA TAAAATGTTG TATTTGCCTT TGAGAGAACG GTATAGAAGA
    CTAAGTGTGG AGGATCTACT CAAAGGAGGG ACACAGGTAT CACGTCTACA CTCTGTGACC
    ACTGCTCTCA ACATCCTTGA GAAGTATGGG AGGAATCTGT TGTCCCCGCT TCGACCCAAA
    TTCTGGAGGA GTGTGAAATT TAACAACCCT GTTTTCAAGA CAACAGTAGA TGCCATTGAG
    GGTGGACGAT TTGTTCTGTC TCTTTATGGC TACTCTCAGT CACTTCCTGA TGGTCTAAGT
    TTTCCAGATT CTGTGCAGGA ACCAGATGTC TCCACTGTTG CTGCTGTAAC AGCTGATGTG
    ATTATTCTTC ACCATGAACT CAACCTACTC ATCTCGAACA TGCACCCACG GCACGAGGAA
    GCAGCACAAG AACTATTAGG TGGTGAACTG CCACAGTCTC TTGTTGCTGA TTACGTAAGT
    GCTCCTAAAG ATCTACAACC AGAAACCACT TCTGCTGGCT GTTTTCTTTG TGGTACTCTA
    GTTTGTTCTG TGTTTTGTTC CTCTTGTAAT GAGATGCTCT GTGAGGAGTG TGATAAGAGA
    GCACACTCTC ATCCAGTCCG TGCCCAACAT GTGCGTTTAC CATGTGGAAA TACAGCAAAG
    CCCAGGTCAT TGGATCAGGA CTCCCCTTCT ACAGTGTCAT CTCGCCCTCC TTGGAGATGT
    TACTCTTGCC ATTTTGAAAA CAAAGGGGGT TCTGTTCTGT GTGTGGTATG TGAGCGCCCC
    CGTGGCTGTA CCAGCAGCCT TTCAAACCTA TCACTTGGAA GTACTTTACA GAGAGACAGC
    TGGGAATGCC AGGCTTGTAC TCTGCAAAAT TCTGCATCTG CAGTTCTGTG TGCAGTGTGT
    GATCGTCCCC GATTGGCAAG CAAACCAGCC CAGGATGGTT ACGATTCTGT TGCTTTGCTT
    TTGAGAGATA TACAGAGGGA TAACAAGAGT ATCACATGCC CTGTGAGTGA AAAAAATGGT
    CTATTTGCCA TGAAGAATCA TCAGGAGAGT ATGCATGAAC CAGAAAGCAT TGGTCCTCTG
    ATAGATCATA CAAGGCAAGA AGAGGAGATT GCTAAAAGTG AGGGCTGGCA GTGTTCCCAT
    TGCACTTTCT TTAACACACG AAATGGACGT GTTTGTGAGA TTTGTGACAG GACCAGTGAG
    GGGACAGCAG CAACTGAACA ACCCAAGGCA CTTGATATGG CACATGTGCA GGAGGAGATC
    CGGCAGAAGC GACTAAGAGA GGATGGAGCT AAAATAGTGG CATTGATCCG GGAAGGAGAG
    AAGTATGGGG TTCTACCTGA AGAGGTGTGT AGCGCAATAC GATATTCTGG TACAGAAGTG
    CCTCAGTGGT GGCTGCAGAC TGAACTTCCC TATGTATTAG AGCGTCTGCT GGATGCTGCT
    TCACAGAAAG CAGGAGATAC AATTGGTGCC CTAACAGTGG AAGAAGCTCG TGATGCTTGG
    GTGTCCAGTG GTGGAGACAT GGATGCTGCT GTCATCTTAT GTGTGACAGA GCGAAGAAGG
    AAGGTTGATG CACTCTCCGG CCTAGGTTTT CCAGAAAGGC ATAAAGTGGT AGCTGCTCTT
    TATGAAAGTG CTGGAGATGT TGGTAGAGCA CTAAGTATAT TACAGAGACC ACTTCTGGAG
    CCTTTTCTTA TACGCATGTG GGAAGAACCA CAACCACCCC TTCGGCTAGA CTCAGCAGAT
    AGACAGGCAG TTCTCCGTAG GTTACTTGCA GAACATTCCT TGCACAGTTG GGGTCGGGCA
    GAGCTAGCTT TGTCATTGCT TATTGAAGGA GAAGGGCGCT ATGAACTCCA GGATGTTGTA
    GAGGCAGTGC GGGAATCGCA GGACAGAGAC TTTATCAAAA GGATGTTAAC ACAGGAGTGT
    GCAGTGTGTG GATGGGAGCT CCCCCGTAAC AAGATGCGTT CTCTAACCTC ATGTGAGTGT
    TGTATCTGTC CTGATTGCTT CCGGATGCAT TTCACTGTAG CAGTGAAAGA GAAACATATT
    AGAGACATGA TATGTCCAGC TTGTGAGGAG CCAGAAATAA GCGACGAGGG GGAGCTTCTT
    CACTATTTCT CTACTCTGGA TATCCTGTTA CGGGACACTT TGGAGGTTGA TGCATATGAT
    TTGTTCCATA AAAAACTAAC TGAAAGAACA CTTATGAAGG ACCCAAAGTT CCTGTGGTGT
    ACACATTGTT CCTTTGGATT CATTTATGAA CGAGATCAGC TGGATGTAAA ATGCCCCCAG
    TGTCAGTGCA GTTTCTGCAG GAAATGCAAG AGGCCTTGGG AAGAACAGCA TCGGTCTTTA
    AATTGTGATG ATTTTCAGAA CTGGAAAAGG GAAAATGATG CAGAATACCA AGCTCAAGGA
    TTGGCTGTAT ACCTGCTAGA GAATGGGATC ACCTGTCCCC ATTGTAAATT TTCTTATGCT
    CTTGCACGAG GGGGATGCAT GCACTTCATA TGTTCTCAGT GTCGCCACCA GTTCTGCAGT
    GGCTGCTATA ACACCTTTCA CCAAAGAAAT AAATGTACAG TCCCGTCCTG TACGGTAAGG
    ATGTCTCTCC ATGCTCACCA TCCTAGAGAC TGTCTCTTTT ACCTCCGAGA TTGGGATGTC
    ACACGTCTGC AAAGACTACT CCAGATGTTA CTGGTACAGT TTAACACAGA TCCACCCGCT
    GGAACACAAA CTGCTCCAGG AGGTGGGTGT CGTGTGATGG AGCAAAAGGA GATACCTGAT
    GGCCTGAGAG ACGAAGCTTG TGGCAAAATA ACACCTCCAG GATATGCTGG TTTGTGCGAG
    TCTCATTATA AAGAATATCT TGTTAGTCGT ATTAATGCCC ATTCTCTGGA TCCATGTGTT
    CTCTATGATC TGGCTGAGAC TATGAATGTG TGTCAGCGCT ACCTGCCTGT ATTGCCTCTA
    CAAGATCCCA CCGAGAATGA GGCCAGCTAC AGGGAGCGTC TCCTGGGGGT GTTAATATCA
    GAGGTGCCAC TGAGTATGAA CATTCCACGG AAGCAGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.