tgfb1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tgfb1.L
Entrez Gene ID	397778
Full Name	transforming growth factor beta 1 L homeolog
Synonyms	TGF-beta5,ced,dpd1,lap,tgf-beta,tgfb,tgfb1,tgfb5,tgfbeta
General protein information	Preferred Names transforming growth factor beta 1 L homeolog Names transforming growth factor beta-1 TGF-beta-1 TGF-beta-5 tgf beta tgf-beta 5 transforming gorwth factor-Beta5 transforming growth factor-B5 transforming growth factor-beta 5 (TGF-beta 5)
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001087861.1	NP_001081330.1	transforming growth factor beta-1 proprotein precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Small C-terminal Domain Phosphatase 3 Dephosphorylates the Linker Sites of Receptor-regulated Smads (R-Smads) to Ensure Transforming Growth Factor ? (TGF?)-mediated Germ Layer Induction in Xenopus Embryos.
Sun G, Hu Z, Min Z, Yan X, Guan Z, Su H, Fu Y, Ma X, Chen YG, Zhang MQ, Tao Q, Wu W
The Journal of biological chemistry290(28)17239-49(2015 Jul)

TGF-? Signaling Regulates the Differentiation of Motile Cilia.
T?zser J, Earwood R, Kato A, Brown J, Tanaka K, Didier R, Megraw TL, Blum M, Kato Y
Cell reports11(7)1000-7(2015 May)

The cell sorting process of Xenopus gastrula cells involves the acto-myosin system and TGF-? signaling.
Harata A, Matsuzaki T, Nishikawa A, Ihara S
In vitro cellular & developmental biology. Animal49(3)220-9(2013 Mar)

Dynamics of TGF-? signaling reveal adaptive and pulsatile behaviors reflected in the nuclear localization of transcription factor Smad4.
Warmflash A, Zhang Q, Sorre B, Vonica A, Siggia ED, Brivanlou AH
Proceedings of the National Academy of Sciences of the United States of America109(28)E1947-56(2012 Jul)

Sortilin associates with transforming growth factor-beta family proteins to enhance lysosome-mediated degradation.
Kwon S, Christian JL
The Journal of biological chemistry286(24)21876-85(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

R-Smads are the key components of TGFbeta beta signals in germ layer induction. SCP3 serves as a vegetally enriched, intrinsic factor to ensure a prepared status of Smads for their activation.
Title: Small C-terminal Domain Phosphatase 3 Dephosphorylates the Linker Sites of Receptor-regulated Smads (R-Smads) to Ensure Transforming Growth Factor ? (TGF?)-mediated Germ Layer Induction in Xenopus Embryos.

These data indicate that TGF-beta signaling is crucial for the function of the transition zone, which in turn may affect the regulation of cilia length.
Title: TGF-? Signaling Regulates the Differentiation of Motile Cilia.

the present in vitro system, which permits not only the cell contraction-mediated cell sorting but also the TGF-b-directed mesodermal induction such as cartilage formation, may fairly reflect the embryogenesis in vivo.
Title: The cell sorting process of Xenopus gastrula cells involves the acto-myosin system and TGF-? signaling.

TGF-beta signaling has a role in nuclear localization of transcription factor Smad4
Title: Dynamics of TGF-? signaling reveal adaptive and pulsatile behaviors reflected in the nuclear localization of transcription factor Smad4.

sortilin negatively regulates TGF-beta signaling by diverting trafficking of precursor proteins to the lysosome during transit through the biosynthetic pathway
Title: Sortilin associates with transforming growth factor-beta family proteins to enhance lysosome-mediated degradation.

The following tgfb1.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tgfb1.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf00820	NM_001087861.1 Latest version!	Xenopus laevis transforming growth factor beta 1 L homeolog (tgfb1.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tgfb1.L ( NM_001087861.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001081330.1 Xenopus laevis transforming growth factor beta 1 L homeolog (tgfb1.L), mRNA.

Price & Availability↑

CloneID	OAf00820
Clone ID Related Accession (Same CDS sequence)	NM_001087861.1
Accession Version	NM_001087861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Xenopus laevis(African clawed frog)
Product	transforming growth factor beta-1 proprotein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J05180.1. On Jul 25, 2007 this sequence version replaced NM_001087862.1. ##Evidence-Data-START## Transcript exon combination :: J05180.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012419 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGTTC TGTGGATGCT GCTGGTGCTA TTGGTCCTGC ATCTTTCCAG TTTAGCTATG 
TCCTTGTCTA CCTGTAAGGC TGTGGATATG GAAGAAGTCA GGAAGAGGAG AATTGAAGCC 
ATCAGGGGCC AAATTCTCAG CAAGCTAAAA CTGGACAAAA CACCAGATGT AGACAGCGAG 
AAGATGACAG TGCCCAGTGA AGCCATCTTC CTTTACAATA GCACCTTGGA GGTGATCAGA 
GAAAAAGCAA CCAGAGAAGA AGAACATGTA GGGCATGATC AGAACATACA GGACTATTAT 
GCAAAACAAG TTTACCGATT TGAAAGCATA ACCGAACTGG AAGACCATGA ATTTAAGTTT 
AAATTTAATG CTTCGCATGT GAGGGAGAAT GTAGGTATGA ACTCTCTTCT GCACCATGCT 
GAGCTGCGCA TGTACAAGAA GCAGACAGAT AAAAACATGG ATCAAAGGAT GGAACTATTC 
TGGAAATACC AGGAAAATGG GACCACTCAC TCAAGATACC TGGAGAGCAA GTACATTACT 
CCAGTAACAG ATGATGAGTG GATGTCATTT GATGTTACAA AGACTGTGAA TGAATGGCTG 
AAACGTGCAG AGGAAAATGA ACAATTCGGG TTGCAGCCTG CCTGCAAATG TCCCACCCCA 
CAGGCCAAAG ATATAGACAT AGAAGGATTT CCTGCTTTAC GTGGAGACTT GGCGAGTTTG 
AGTTCCAAAG AAAACACGAA ACCCTACCTG ATGATCACTT CGATGCCAGC TGAGCGTATT 
GACACAGTTA CAAGCTCACG GAAAAAGCGA GGGGTTGGAC AGGAGTATTG CTTTGGGAAC 
AATGGGCCAA ACTGCTGTGT GAAACCTCTT TACATAAATT TTCGGAAGGA TCTAGGCTGG 
AAGTGGATCC ATGAGCCTAA GGGATATGAA GCAAATTATT GTTTAGGAAA TTGTCCTTAC 
ATCTGGAGCA TGGATACTCA GTACAGCAAG GTGCTATCAC TTTATAATCA GAACAATCCC 
GGTGCATCTA TATCTCCCTG CTGTGTTCCT GATGTCTTGG AGCCACTGCC AATCATTTAT 
TATGTTGGCC GCACTGCTAA AGTAGAGCAG CTCTCTAATA TGGTGGTAAG GTCTTGCAAC 
TGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001081330.1
CDS	504..1652
Translation	MEVLWMLLVLLVLHLSSLAMSLSTCKAVDMEEVRKRRIEAIRGQILSKLKLDKTPDVDSEKMTVPSEAIFLYNSTLEVIREKATREEEHVGHDQNIQDYYAKQVYRFESITELEDHEFKFKFNASHVRENVGMNSLLHHAELRMYKKQTDKNMDQRMELFWKYQENGTTHSRYLESKYITPVTDDEWMSFDVTKTVNEWLKRAEENEQFGLQPACKCPTPQAKDIDIEGFPALRGDLASLSSKENTKPYLMITSMPAERIDTVTSSRKKRGVGQEYCFGNNGPNCCVKPLYINFRKDLGWKWIHEPKGYEANYCLGNCPYIWSMDTQYSKVLSLYNQNNPGASISPCCVPDVLEPLPIIYYVGRTAKVEQLSNMVVRSCNCS

Target ORF information:

RefSeq Version	NM_001087861.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis transforming growth factor beta 1 L homeolog (tgfb1.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001087861.1

ORF Insert Sequence:

ATGGAGGTTC TGTGGATGCT GCTGGTGCTA TTGGTCCTGC ATCTTTCCAG TTTAGCTATG 
TCCTTGTCTA CCTGTAAGGC TGTGGATATG GAAGAAGTCA GGAAGAGGAG AATTGAAGCC 
ATCAGGGGCC AAATTCTCAG CAAGCTAAAA CTGGACAAAA CACCAGATGT AGACAGCGAG 
AAGATGACAG TGCCCAGTGA AGCCATCTTC CTTTACAATA GCACCTTGGA GGTGATCAGA 
GAAAAAGCAA CCAGAGAAGA AGAACATGTA GGGCATGATC AGAACATACA GGACTATTAT 
GCAAAACAAG TTTACCGATT TGAAAGCATA ACCGAACTGG AAGACCATGA ATTTAAGTTT 
AAATTTAATG CTTCGCATGT GAGGGAGAAT GTAGGTATGA ACTCTCTTCT GCACCATGCT 
GAGCTGCGCA TGTACAAGAA GCAGACAGAT AAAAACATGG ATCAAAGGAT GGAACTATTC 
TGGAAATACC AGGAAAATGG GACCACTCAC TCAAGATACC TGGAGAGCAA GTACATTACT 
CCAGTAACAG ATGATGAGTG GATGTCATTT GATGTTACAA AGACTGTGAA TGAATGGCTG 
AAACGTGCAG AGGAAAATGA ACAATTCGGG TTGCAGCCTG CCTGCAAATG TCCCACCCCA 
CAGGCCAAAG ATATAGACAT AGAAGGATTT CCTGCTTTAC GTGGAGACTT GGCGAGTTTG 
AGTTCCAAAG AAAACACGAA ACCCTACCTG ATGATCACTT CGATGCCAGC TGAGCGTATT 
GACACAGTTA CAAGCTCACG GAAAAAGCGA GGGGTTGGAC AGGAGTATTG CTTTGGGAAC 
AATGGGCCAA ACTGCTGTGT GAAACCTCTT TACATAAATT TTCGGAAGGA TCTAGGCTGG 
AAGTGGATCC ATGAGCCTAA GGGATATGAA GCAAATTATT GTTTAGGAAA TTGTCCTTAC 
ATCTGGAGCA TGGATACTCA GTACAGCAAG GTGCTATCAC TTTATAATCA GAACAATCCC 
GGTGCATCTA TATCTCCCTG CTGTGTTCCT GATGTCTTGG AGCCACTGCC AATCATTTAT 
TATGTTGGCC GCACTGCTAA AGTAGAGCAG CTCTCTAATA TGGTGGTAAG GTCTTGCAAC 
TGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Small C-terminal Domain Phosphatase 3 Dephosphorylates the Linker Sites of Receptor-regulated Smads (R-Smads) to Ensure Transforming Growth Factor ? (TGF?)-mediated Germ Layer Induction in Xenopus Embryos.
The Journal of biological chemistry290(28)17239-49(2015 Jul)
Sun G,Hu Z,Min Z,Yan X,Guan Z,Su H,Fu Y,Ma X,Chen YG,Zhang MQ,Tao Q,Wu W

TGF-? Signaling Regulates the Differentiation of Motile Cilia.
Cell reports11(7)1000-7(2015 May)
T?zser J,Earwood R,Kato A,Brown J,Tanaka K,Didier R,Megraw TL,Blum M,Kato Y

The cell sorting process of Xenopus gastrula cells involves the acto-myosin system and TGF-? signaling.
In vitro cellular & developmental biology. Animal49(3)220-9(2013 Mar)
Harata A,Matsuzaki T,Nishikawa A,Ihara S

Dynamics of TGF-? signaling reveal adaptive and pulsatile behaviors reflected in the nuclear localization of transcription factor Smad4.
Proceedings of the National Academy of Sciences of the United States of America109(28)E1947-56(2012 Jul)
Warmflash A,Zhang Q,Sorre B,Vonica A,Siggia ED,Brivanlou AH

Sortilin associates with transforming growth factor-beta family proteins to enhance lysosome-mediated degradation.
The Journal of biological chemistry286(24)21876-85(2011 Jun)
Kwon S,Christian JL