mcm4.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mcm4.S
Entrez Gene ID	373601
Full Name	minichromosome maintenance complex component 4 S homeolog
Synonyms	cdc21,mcm4,mcm4-a,mcm4-b,mcm4.L
General protein information	Preferred Names minichromosome maintenance complex component 4 S homeolog Names DNA replication licensing factor mcm4-A CDC21 homolog-A P1-CDC21-A S.pombe cdc21+ homologue minichromosome maintenance complex component 4 L homeolog minichromosome maintenance deficient 4 minichromosome maintenance protein 4-A p98 xMCM4-A
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018268549.1	XP_018124038.1	DNA replication licensing factor mcm4-A isoform X1
NM_001085600.2	NP_001079069.1	DNA replication licensing factor mcm4-A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Cdc7-Drf1 is a developmentally regulated protein kinase required for the initiation of vertebrate DNA replication.
Takahashi TS, Walter JC
Genes & development19(19)2295-300(2005 Oct)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides.
Th?mmes P, Kubota Y, Takisawa H, Blow JJ
The EMBO journal16(11)3312-9(1997 Jun)

Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs.
Kubota Y, Mimura S, Nishimoto S, Masuda T, Nojima H, Takisawa H
The EMBO journal16(11)3320-31(1997 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mcm4.S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mcm4.S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf03413	NM_001085600.2 Latest version!	Xenopus laevis minichromosome maintenance complex component 4 S homeolog (mcm4.S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAf36400	XM_018268549.1 Latest version!	Xenopus laevis minichromosome maintenance complex component 4 L homeolog (mcm4.L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mcm4.S ( NM_001085600.2 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001079069.1 Xenopus laevis minichromosome maintenance complex component 4 S homeolog (mcm4.S), mRNA.

Price & Availability↑

CloneID	OAf03413
Clone ID Related Accession (Same CDS sequence)	NM_001085600.2
Accession Version	NM_001085600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-08
Organism	Xenopus laevis(African clawed frog)
Product	DNA replication licensing factor mcm4-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U44049.1. On May 6, 2010 this sequence version replaced NM_001085600.1. ##Evidence-Data-START## Transcript exon combination :: U44049.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02851412, SAMN02851414 [ECO:0000348] ##Evidence-Data-END##

ATGTCATCTC CAACATCCAC ACCAAGCCGT AGAAGAGTAA ACGTGGGCGA GGCAGTAACC 
CTCCAACTCC TCGTGGAGAG GAAGTGCAGT CTCCTCCGTC ACAGAAAAGA CGGACAGAAG 
ATTCCACATC AATTGGTGAG CTTCTGCCCA TGCCAACCTC ACCTTCAGGA GACATTCAGA 
GTCCCTTGTT TTCTAGCCCT TGCACCATCA AGGCATTCAG CCCATCAAAG TGAGCTTGAT 
CTAAGCTCTC CTCTGACTTA TGGCACCCCT AGCTCAAGGG TTGAGGGTAC CCCAAGAAGT 
GGTATCAGAG GGACTCCTGC TCGTCAGAGA GCTGACCTGG GATCAGCACG CAAAGTTAAG 
CAGGTGGATT TACATTCAGA CCAGCCTGCT GCAGAGGAGC TAGTTACGAG TGAGCAATCT 
TTGGGACAGA AGTTGGTGAT CTGGGGAACT GATGTCAATG TTGCCATATG CAAAGAAAAG 
TTTCAGCGTT TTGTTCAGCG ATTCATTGAT CCTTTGGCCA AAGAAGAGGA AAATGTAGGG 
CTGGATCTGA ATGAGCCTAT ATATATGCAA CGACTTGAAG AGATAAATGT GGTAGGCGAG 
CCATTTCTAA ACATTGACTG TGACCATCTC AGAAGCTTTG ACCAGGATCT TTACAGACAA 
CTAGTGTGCT ACCCACAGGA AGTTATACCA ACATTTGATA TGGCTGCTAA TGAGATCTTC 
TTTGAGAGGT ACCCTGACTC CATTTTGGAG CACCAGATTC AAGTTCGACC CTATAATGCA 
TTGAAAACAA GAAATATGAG AAGCCTAAAT CCTGAAGATA TTGATCAACT TATCACAATT 
AGTGGCATGG TTATCAGAAC TTCTCAAATC ATCCCTGAGA TGCAGGAATC ATTTTTTAAA 
TGTCAAGTGT GTGCCTTCAC TACTCGGGTG GAGATTGATC GTGGGCGCAT TGCAGAGCCA 
TCTGTCTGCA AACACTGCAA CACCACACAC AGCATGGCTC TTATTCACAA TCGCTCTATG 
TTTTCAGACA AGCAAATGAT TAAACTGCAA GAGTCTCCAG GAGATATGCC TGCTGGTCAG 
ACACCTCATA CAACCATTCT CTATGCTCAT AATGACTTGG TAGATAAGGT ACAGCCTGGT 
GATAGAGTGA ATGTCACAGG TATTTATAGA GCTGTCCCAA TTCGAGTTAA CCCTAGAGTT 
CGAAATGTAA AGTCGGTTTA CAAAACTCAC ATTGATGTAA TTCATTACCG AAAAACGGAT 
TCAAAGCGGT TGCATGGTAT TGATGAGGAC ACTGAACAGA AGATGTTCAC TGAAGAAAGG 
GTTGCAGTGT TAAAGGAACT GGCAGCAAAG CCAGACATCT ACGAGAGGCT TGCTGCAGCT 
CTTGCACCAA GTATATATGA GCATGAAGAT ATAAAAAAGG GAATTTTACT TCAGTTGTTT 
GGTGGGACAC GGAAGGACTT CAGCCATACT GGAAGAGGCA AATTCCGTGC TGAAGTAAAC 
ATACTTTTGT GTGGTGATCC AGGAACCAGT AAATCTCAGC TTCTCCAATA CGTATATAAT 
CTGGTACCTA GAGGCCAGTA CACCTCTGGA AAGGGATCCA GCGCAGTTGG ATTAACCGCA 
TATGTGATGA AGGACCCAGA AACGAGGCAG TTAGTACTGC AGACTGGCGC CTTGGTTCTT 
AGTGACAATG GCATTTGCTG CATTGATGAA TTTGACAAAA TGAACGAGAG CACTAGATCT 
GTCCTACATG AAGTAATGGA ACAGCAAACC CTCTCCATTG CAAAGGCTGG AATAATCTGT 
CAACTAAATG CACGTACCTC CGTTTTGGCT GCTGCAAACC CAGTGGAGTC ACAATGGAAC 
CCCAAGAAAA CCACTATAGA AAATATACAG CTTCCACATA CACTCTTATC AAGGTTTGAT 
CTAATATTTT TGATGCTGGA TCCACAAGAT GAAACATATG ACCGCCGCTT AGCCCACCAC 
CTTGTGGTTT TGTACTACCA GAGTGAGGAG CAATTGAAAG AAGAGCACTT AGACATGGCA 
GTATTAAAGG ATTATATTGC ATATGCTCGC ACCTACGTGA ATCCAAGACT TGGTGAAGAA 
GCAAGCCAGG CTCTAATAGA GGCCTATGTA GACATGAGGA AAATCGGCAG TGGTCGTGGA 
ATGGTTTCTG CCTATCCCAG ACAACTCGAG TCTCTAATAC GCTTATCAGA AGCGCATGCA 
AAAGTACGCT TTTCTAGCAA GGTGGAAACA ATTGATGTTG AAGAGGCAAA ACGTCTTCAT 
CGTGAGGCTT TGAAACAGTC TGCCACTGAT CCCAGAACTG GCATTGTTGA TATATCTATT 
CTCACAACAG GAATGAGTGC AACTGCTCGA AAACGCAAAG AAGAATTGGC CCAGGTTTTA 
AAGAAGCTTA TCCAGTCCAA GGGCAAAACT CCAGCTTTCA AATACCAGCA GCTTTTTGAG 
GATCTACGTG GGCAGTCTGA TGCAGCCATT ACAAAAGACA TGTTTGATGA AGCTTTGCAT 
GCACTTGCAG ATGAAGACTA CTTAACAGTG ACTGGAAAGA CTGTAAGGTT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001079069.1
CDS	185..2761
Translation	MSSPTSTPSRRRVNVGEAVTLQLLVERKCSLLRHRKDGQKIPHQLVSFCPCQPHLQETFRVPCFLALAPSRHSAHQSELDLSSPLTYGTPSSRVEGTPRSGIRGTPARQRADLGSARKVKQVDLHSDQPAAEELVTSEQSLGQKLVIWGTDVNVAICKEKFQRFVQRFIDPLAKEEENVGLDLNEPIYMQRLEEINVVGEPFLNIDCDHLRSFDQDLYRQLVCYPQEVIPTFDMAANEIFFERYPDSILEHQIQVRPYNALKTRNMRSLNPEDIDQLITISGMVIRTSQIIPEMQESFFKCQVCAFTTRVEIDRGRIAEPSVCKHCNTTHSMALIHNRSMFSDKQMIKLQESPGDMPAGQTPHTTILYAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVRNVKSVYKTHIDVIHYRKTDSKRLHGIDEDTEQKMFTEERVAVLKELAAKPDIYERLAAALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEVNILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPVESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDETYDRRLAHHLVVLYYQSEEQLKEEHLDMAVLKDYIAYARTYVNPRLGEEASQALIEAYVDMRKIGSGRGMVSAYPRQLESLIRLSEAHAKVRFSSKVETIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATARKRKEELAQVLKKLIQSKGKTPAFKYQQLFEDLRGQSDAAITKDMFDEALHALADEDYLTVTGKTVRLL

Target ORF information:

RefSeq Version	NM_001085600.2
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis minichromosome maintenance complex component 4 S homeolog (mcm4.S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085600.2

ORF Insert Sequence:

ATGTCATCTC CAACATCCAC ACCAAGCCGT AGAAGAGTAA ACGTGGGCGA GGCAGTAACC 
CTCCAACTCC TCGTGGAGAG GAAGTGCAGT CTCCTCCGTC ACAGAAAAGA CGGACAGAAG 
ATTCCACATC AATTGGTGAG CTTCTGCCCA TGCCAACCTC ACCTTCAGGA GACATTCAGA 
GTCCCTTGTT TTCTAGCCCT TGCACCATCA AGGCATTCAG CCCATCAAAG TGAGCTTGAT 
CTAAGCTCTC CTCTGACTTA TGGCACCCCT AGCTCAAGGG TTGAGGGTAC CCCAAGAAGT 
GGTATCAGAG GGACTCCTGC TCGTCAGAGA GCTGACCTGG GATCAGCACG CAAAGTTAAG 
CAGGTGGATT TACATTCAGA CCAGCCTGCT GCAGAGGAGC TAGTTACGAG TGAGCAATCT 
TTGGGACAGA AGTTGGTGAT CTGGGGAACT GATGTCAATG TTGCCATATG CAAAGAAAAG 
TTTCAGCGTT TTGTTCAGCG ATTCATTGAT CCTTTGGCCA AAGAAGAGGA AAATGTAGGG 
CTGGATCTGA ATGAGCCTAT ATATATGCAA CGACTTGAAG AGATAAATGT GGTAGGCGAG 
CCATTTCTAA ACATTGACTG TGACCATCTC AGAAGCTTTG ACCAGGATCT TTACAGACAA 
CTAGTGTGCT ACCCACAGGA AGTTATACCA ACATTTGATA TGGCTGCTAA TGAGATCTTC 
TTTGAGAGGT ACCCTGACTC CATTTTGGAG CACCAGATTC AAGTTCGACC CTATAATGCA 
TTGAAAACAA GAAATATGAG AAGCCTAAAT CCTGAAGATA TTGATCAACT TATCACAATT 
AGTGGCATGG TTATCAGAAC TTCTCAAATC ATCCCTGAGA TGCAGGAATC ATTTTTTAAA 
TGTCAAGTGT GTGCCTTCAC TACTCGGGTG GAGATTGATC GTGGGCGCAT TGCAGAGCCA 
TCTGTCTGCA AACACTGCAA CACCACACAC AGCATGGCTC TTATTCACAA TCGCTCTATG 
TTTTCAGACA AGCAAATGAT TAAACTGCAA GAGTCTCCAG GAGATATGCC TGCTGGTCAG 
ACACCTCATA CAACCATTCT CTATGCTCAT AATGACTTGG TAGATAAGGT ACAGCCTGGT 
GATAGAGTGA ATGTCACAGG TATTTATAGA GCTGTCCCAA TTCGAGTTAA CCCTAGAGTT 
CGAAATGTAA AGTCGGTTTA CAAAACTCAC ATTGATGTAA TTCATTACCG AAAAACGGAT 
TCAAAGCGGT TGCATGGTAT TGATGAGGAC ACTGAACAGA AGATGTTCAC TGAAGAAAGG 
GTTGCAGTGT TAAAGGAACT GGCAGCAAAG CCAGACATCT ACGAGAGGCT TGCTGCAGCT 
CTTGCACCAA GTATATATGA GCATGAAGAT ATAAAAAAGG GAATTTTACT TCAGTTGTTT 
GGTGGGACAC GGAAGGACTT CAGCCATACT GGAAGAGGCA AATTCCGTGC TGAAGTAAAC 
ATACTTTTGT GTGGTGATCC AGGAACCAGT AAATCTCAGC TTCTCCAATA CGTATATAAT 
CTGGTACCTA GAGGCCAGTA CACCTCTGGA AAGGGATCCA GCGCAGTTGG ATTAACCGCA 
TATGTGATGA AGGACCCAGA AACGAGGCAG TTAGTACTGC AGACTGGCGC CTTGGTTCTT 
AGTGACAATG GCATTTGCTG CATTGATGAA TTTGACAAAA TGAACGAGAG CACTAGATCT 
GTCCTACATG AAGTAATGGA ACAGCAAACC CTCTCCATTG CAAAGGCTGG AATAATCTGT 
CAACTAAATG CACGTACCTC CGTTTTGGCT GCTGCAAACC CAGTGGAGTC ACAATGGAAC 
CCCAAGAAAA CCACTATAGA AAATATACAG CTTCCACATA CACTCTTATC AAGGTTTGAT 
CTAATATTTT TGATGCTGGA TCCACAAGAT GAAACATATG ACCGCCGCTT AGCCCACCAC 
CTTGTGGTTT TGTACTACCA GAGTGAGGAG CAATTGAAAG AAGAGCACTT AGACATGGCA 
GTATTAAAGG ATTATATTGC ATATGCTCGC ACCTACGTGA ATCCAAGACT TGGTGAAGAA 
GCAAGCCAGG CTCTAATAGA GGCCTATGTA GACATGAGGA AAATCGGCAG TGGTCGTGGA 
ATGGTTTCTG CCTATCCCAG ACAACTCGAG TCTCTAATAC GCTTATCAGA AGCGCATGCA 
AAAGTACGCT TTTCTAGCAA GGTGGAAACA ATTGATGTTG AAGAGGCAAA ACGTCTTCAT 
CGTGAGGCTT TGAAACAGTC TGCCACTGAT CCCAGAACTG GCATTGTTGA TATATCTATT 
CTCACAACAG GAATGAGTGC AACTGCTCGA AAACGCAAAG AAGAATTGGC CCAGGTTTTA 
AAGAAGCTTA TCCAGTCCAA GGGCAAAACT CCAGCTTTCA AATACCAGCA GCTTTTTGAG 
GATCTACGTG GGCAGTCTGA TGCAGCCATT ACAAAAGACA TGTTTGATGA AGCTTTGCAT 
GCACTTGCAG ATGAAGACTA CTTAACAGTG ACTGGAAAGA CTGTAAGGTT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcm4.S ( XM_018268549.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018124038.1 Xenopus laevis minichromosome maintenance complex component 4 L homeolog (mcm4.L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf36400
Clone ID Related Accession (Same CDS sequence)	XM_018268549.1
Accession Version	XM_018268549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-19
Organism	Xenopus laevis(African clawed frog)
Product	DNA replication licensing factor mcm4-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030735.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCTC CAACATCCAC ACCAAGCCGT AGGAAGAGTA AACGTGGGCG AGGCAGTAAC 
CCTCCAACTC CTCGTGGAGA GGAAGTGCAG TCTCCTCCGT CACAGAAAAG ACGGACAGAA 
GATTCCACAT CAATTGGTGA GCTTCTGCCC ATGCCAACCT CACCTTCAGG AGACATTCAG 
AGTCCCTTGT TTTCTAGCCC TGCACCATCA AGGCATTCAG CCCATCAAAG TGAGCTTGAT 
CTAAGCTCTC CTCTGACTTA TGGCACCCCT AGCTCAAGGG TTGAGAGTAC CCCAAGAAGT 
GGTATCAGAG GGACTCCTGC TCGTCAGAGA CCTGACCTGG GATCAGCACG CAAAGTTAAG 
CAGGTGGATT TACATTCAGA CCAGCCTGCT GCAGAGGAGC TAGTTACGAG TGAGCAATCT 
TTGGGACAGA AGTTGGTGAT CTGGGGAACT GATGTCAATG TTGCCATATG CAAAGAAAAG 
TTTCAGCGTT TTGTTCAGCG ATTCATTGAT CCTTTGGCCA AAGAAGAGGA AAATGTAGGG 
CTGGATCTGA ATGAGCCTAT CTATATGCAA CGACTTGAAG AGATAAATGT GGTAGGCGAG 
CCATTTCTAA ACATTGACTG TGACCATCTC AGAAGCTTTG ACCAGGATCT TTACAGACAA 
CTAGTGTGCT ACCCACAGGA AGTTATACCA ACATTTGATA TGGCTGCTAA TGAGATCTTC 
TTTGAGCGGT ACCCTGACTC CATTTTGGAG CACCAGATTC AAGTTCGACC CTATAATGCA 
TTGAAAACAA GAAATATGAG AAGCCTAAAT CCTGAAGATA TTGATCAACT TATCACAATT 
AGTGGCATGG TTATCAGAAC TTCTCAAATC ATCCCTGAGA TGCAGGAATC ATTTTTTAAA 
TGTCAAGTGT GTGCCTTCAC TACTCGGGTG GAGATTGATC GTGGGCGCAT TGCAGAGCCA 
TCTGTCTGCA AACACTGCAA CACCACACAC AGCATGGCTC TTATTCACAA TCGCTCTATG 
TTTTCAGACA AGCAAATGAT TAAACTGCAA GAGTCTCCAG AAGATATGCC TGCTGGTCAG 
ACACCTCATA CAACCATTCT CTATGCTCAT AATGACTTGG TAGATAAGGT ACAGCCTGGT 
GATAGAGTGA ATGTCACAGG TATTTATAGA GCTGTCCCAA TTCGAGTTAA CCCTAGAGTT 
CGAAATGTAA AGTCGGTTTA CAAAACTCAC ATTGATGTAA TTCATTACCG AAAAACGGAT 
TCAAAGCGGT TGCATGGTAT TGATGAGGAC ACTGAACAGA AGATGTTCAC TGAAGAAAGG 
GTTGCAGTGT TAAAGGAACT GGCAGCAAAG CCAGACATCT ACGAGAGGCT TGCTGCAGCT 
CTTGCACCAA GTATATATGA GCATGAAGAT ATAAAAAAGG GAATTTTACT TCAGTTGTTT 
GGTGGGACAC GGAAGGACTT TAGCCATACT GGAAGAGGCA AATTCCGTGC TGAAGTAAAC 
ATACTTTTGT GTGGTGATCC AGGAACCAGT AAATCTCAGC TTCTCCAATA CGTATATAAT 
CTGGTACCTA GAGGCCAGTA CACCTCTGGA AAGGGATCCA GCGCAGTTGG ATTAACCGCA 
TATGTGATGA AGGACCCAGA AACGAGGCAG TTGGTACTGC AGACTGGCGC CTTGGTTCTT 
AGTGACAATG GCATTTGCTG CATTGATGAA TTTGACAAAA TGAACGAGAG CACTAGATCT 
GTCCTACATG AAGTAATGGA ACAGCAAACC CTCTCCATTG CAAAGGCTGG AATAATCTGT 
CAACTAAATG CACGTACCTC CGTTTTGGCT GCTGCAAACC CAGTGGAGTC ACAATGGAAC 
CCCAAGAAAA CCACTATAGA AAATATACAG CTTCCACATA CACTCTTATC AAGGTTTGAT 
CTAATATTTT TGATGCTGGA TCCACAAGAT GAAACATATG ACCGCCGCTT AGCCCACCAC 
CTTGTGGCTT TGTACTACCA GAGTGAGGAG CAATTGAAAG AAGAGCACTT AGACATGGCA 
GCATTAAAGG ATTATATTGC ATATGCTCGC ACCTACGTGA ATCCAAGACT TGGTGAAGAA 
GCAAGCCAGG CTCTAATAGA GGCCTATGTA GACATGAGGA AAATCGGCAG TGGTCGTGGA 
ATGGTTTCTG CCTATCCCAG ACAACTCGAG TCTCTAATAC GCTTATCAGA AGCGCATGCA 
AAAGTACGCT TTTCTAGCAA GGTGGAAACA ATTGATGTTG AAGAGGCAAA ACGTCTTCAT 
CGTGAGGCTT TGAAACAGTC TGCCACTGAT CCCAGAACTG GCATTGTTGA TATATCTATT 
CTCACAACAG GAATGAGTGC AACTGCTCGA AAACGTAAAG AAGAATTGGC CCAGGTTTTA 
AAGAAGCTTA TCCAGTCCAA GGGCAAAACT CCAGCTTTCA AATACCAGCA GCTTTTTGAG 
GATCTACGTG GGCAGTCTGA TGCAGCCATT ACAAAAGACA TGTTTGATGA AGCTTTGCAT 
GCACTTGCAG ATGAAGACTA CTTAACAGTG ACTGGAAAGA CTGTAAGGTT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018124038.1
CDS	451..3027
Translation	MSSPTSTPSRRKSKRGRGSNPPTPRGEEVQSPPSQKRRTEDSTSIGELLPMPTSPSGDIQSPLFSSPAPSRHSAHQSELDLSSPLTYGTPSSRVESTPRSGIRGTPARQRPDLGSARKVKQVDLHSDQPAAEELVTSEQSLGQKLVIWGTDVNVAICKEKFQRFVQRFIDPLAKEEENVGLDLNEPIYMQRLEEINVVGEPFLNIDCDHLRSFDQDLYRQLVCYPQEVIPTFDMAANEIFFERYPDSILEHQIQVRPYNALKTRNMRSLNPEDIDQLITISGMVIRTSQIIPEMQESFFKCQVCAFTTRVEIDRGRIAEPSVCKHCNTTHSMALIHNRSMFSDKQMIKLQESPEDMPAGQTPHTTILYAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVRNVKSVYKTHIDVIHYRKTDSKRLHGIDEDTEQKMFTEERVAVLKELAAKPDIYERLAAALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEVNILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPVESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDETYDRRLAHHLVALYYQSEEQLKEEHLDMAALKDYIAYARTYVNPRLGEEASQALIEAYVDMRKIGSGRGMVSAYPRQLESLIRLSEAHAKVRFSSKVETIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATARKRKEELAQVLKKLIQSKGKTPAFKYQQLFEDLRGQSDAAITKDMFDEALHALADEDYLTVTGKTVRLL

Target ORF information:

RefSeq Version	XM_018268549.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis minichromosome maintenance complex component 4 L homeolog (mcm4.L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018268549.1

ORF Insert Sequence:

ATGTCATCTC CAACATCCAC ACCAAGCCGT AGGAAGAGTA AACGTGGGCG AGGCAGTAAC 
CCTCCAACTC CTCGTGGAGA GGAAGTGCAG TCTCCTCCGT CACAGAAAAG ACGGACAGAA 
GATTCCACAT CAATTGGTGA GCTTCTGCCC ATGCCAACCT CACCTTCAGG AGACATTCAG 
AGTCCCTTGT TTTCTAGCCC TGCACCATCA AGGCATTCAG CCCATCAAAG TGAGCTTGAT 
CTAAGCTCTC CTCTGACTTA TGGCACCCCT AGCTCAAGGG TTGAGAGTAC CCCAAGAAGT 
GGTATCAGAG GGACTCCTGC TCGTCAGAGA CCTGACCTGG GATCAGCACG CAAAGTTAAG 
CAGGTGGATT TACATTCAGA CCAGCCTGCT GCAGAGGAGC TAGTTACGAG TGAGCAATCT 
TTGGGACAGA AGTTGGTGAT CTGGGGAACT GATGTCAATG TTGCCATATG CAAAGAAAAG 
TTTCAGCGTT TTGTTCAGCG ATTCATTGAT CCTTTGGCCA AAGAAGAGGA AAATGTAGGG 
CTGGATCTGA ATGAGCCTAT CTATATGCAA CGACTTGAAG AGATAAATGT GGTAGGCGAG 
CCATTTCTAA ACATTGACTG TGACCATCTC AGAAGCTTTG ACCAGGATCT TTACAGACAA 
CTAGTGTGCT ACCCACAGGA AGTTATACCA ACATTTGATA TGGCTGCTAA TGAGATCTTC 
TTTGAGCGGT ACCCTGACTC CATTTTGGAG CACCAGATTC AAGTTCGACC CTATAATGCA 
TTGAAAACAA GAAATATGAG AAGCCTAAAT CCTGAAGATA TTGATCAACT TATCACAATT 
AGTGGCATGG TTATCAGAAC TTCTCAAATC ATCCCTGAGA TGCAGGAATC ATTTTTTAAA 
TGTCAAGTGT GTGCCTTCAC TACTCGGGTG GAGATTGATC GTGGGCGCAT TGCAGAGCCA 
TCTGTCTGCA AACACTGCAA CACCACACAC AGCATGGCTC TTATTCACAA TCGCTCTATG 
TTTTCAGACA AGCAAATGAT TAAACTGCAA GAGTCTCCAG AAGATATGCC TGCTGGTCAG 
ACACCTCATA CAACCATTCT CTATGCTCAT AATGACTTGG TAGATAAGGT ACAGCCTGGT 
GATAGAGTGA ATGTCACAGG TATTTATAGA GCTGTCCCAA TTCGAGTTAA CCCTAGAGTT 
CGAAATGTAA AGTCGGTTTA CAAAACTCAC ATTGATGTAA TTCATTACCG AAAAACGGAT 
TCAAAGCGGT TGCATGGTAT TGATGAGGAC ACTGAACAGA AGATGTTCAC TGAAGAAAGG 
GTTGCAGTGT TAAAGGAACT GGCAGCAAAG CCAGACATCT ACGAGAGGCT TGCTGCAGCT 
CTTGCACCAA GTATATATGA GCATGAAGAT ATAAAAAAGG GAATTTTACT TCAGTTGTTT 
GGTGGGACAC GGAAGGACTT TAGCCATACT GGAAGAGGCA AATTCCGTGC TGAAGTAAAC 
ATACTTTTGT GTGGTGATCC AGGAACCAGT AAATCTCAGC TTCTCCAATA CGTATATAAT 
CTGGTACCTA GAGGCCAGTA CACCTCTGGA AAGGGATCCA GCGCAGTTGG ATTAACCGCA 
TATGTGATGA AGGACCCAGA AACGAGGCAG TTGGTACTGC AGACTGGCGC CTTGGTTCTT 
AGTGACAATG GCATTTGCTG CATTGATGAA TTTGACAAAA TGAACGAGAG CACTAGATCT 
GTCCTACATG AAGTAATGGA ACAGCAAACC CTCTCCATTG CAAAGGCTGG AATAATCTGT 
CAACTAAATG CACGTACCTC CGTTTTGGCT GCTGCAAACC CAGTGGAGTC ACAATGGAAC 
CCCAAGAAAA CCACTATAGA AAATATACAG CTTCCACATA CACTCTTATC AAGGTTTGAT 
CTAATATTTT TGATGCTGGA TCCACAAGAT GAAACATATG ACCGCCGCTT AGCCCACCAC 
CTTGTGGCTT TGTACTACCA GAGTGAGGAG CAATTGAAAG AAGAGCACTT AGACATGGCA 
GCATTAAAGG ATTATATTGC ATATGCTCGC ACCTACGTGA ATCCAAGACT TGGTGAAGAA 
GCAAGCCAGG CTCTAATAGA GGCCTATGTA GACATGAGGA AAATCGGCAG TGGTCGTGGA 
ATGGTTTCTG CCTATCCCAG ACAACTCGAG TCTCTAATAC GCTTATCAGA AGCGCATGCA 
AAAGTACGCT TTTCTAGCAA GGTGGAAACA ATTGATGTTG AAGAGGCAAA ACGTCTTCAT 
CGTGAGGCTT TGAAACAGTC TGCCACTGAT CCCAGAACTG GCATTGTTGA TATATCTATT 
CTCACAACAG GAATGAGTGC AACTGCTCGA AAACGTAAAG AAGAATTGGC CCAGGTTTTA 
AAGAAGCTTA TCCAGTCCAA GGGCAAAACT CCAGCTTTCA AATACCAGCA GCTTTTTGAG 
GATCTACGTG GGCAGTCTGA TGCAGCCATT ACAAAAGACA TGTTTGATGA AGCTTTGCAT 
GCACTTGCAG ATGAAGACTA CTTAACAGTG ACTGGAAAGA CTGTAAGGTT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cdc7-Drf1 is a developmentally regulated protein kinase required for the initiation of vertebrate DNA replication.
Genes & development19(19)2295-300(2005 Oct)
Takahashi TS,Walter JC

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides.
The EMBO journal16(11)3312-9(1997 Jun)
Th?mmes P,Kubota Y,Takisawa H,Blow JJ

Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs.
The EMBO journal16(11)3320-31(1997 Jun)
Kubota Y,Mimura S,Nishimoto S,Masuda T,Nojima H,Takisawa H