tnf.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tnf.L
Entrez Gene ID	100137625
Full Name	tumor necrosis factor L homeolog
Synonyms	dif,tnf,tnf-alpha,tnfa,tnfsf2,xtnf,xtnf-alpha
General protein information	Preferred Names tumor necrosis factor L homeolog Names tumor necrosis factor L homeolog tumor necrosis factor (TNF superfamily, member 2) tumor necrosis factor alpha
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001114778.1	NP_001108250.1	tumor necrosis factor L homeolog

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Tumor necrosis factor-alpha attenuates thyroid hormone-induced apoptosis in vascular endothelial cell line XLgoo established from Xenopus tadpole tails.
Mawaribuchi S, Tamura K, Okano S, Takayama S, Yaoita Y, Shiba T, Takamatsu N, Ito M
Endocrinology149(7)3379-89(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tnf.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tnf.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf04486	NM_001114778.1 Latest version!	Xenopus laevis tumor necrosis factor L homeolog (tnf.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tnf.L ( NM_001114778.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001108250.1 Xenopus laevis tumor necrosis factor L homeolog (tnf.L), mRNA.

Price & Availability↑

CloneID	OAf04486
Clone ID Related Accession (Same CDS sequence)	NM_001114778.1
Accession Version	NM_001114778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-28
Organism	Xenopus laevis(African clawed frog)
Product	tumor necrosis factor L homeolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB298595.1. ##Evidence-Data-START## Transcript exon combination :: AB298595.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04158975 [ECO:0000348] ##Evidence-Data-END##

ATGAATAGTG TAGAGTCACA GATGGAGAAT GGGCTCCTAA TTGTCAGGCA GGAAAGAAGC 
AACAGGAATT CCACCTGGCG CTTTGTCAGT ATCTGTGCAT TCCTGTTATT ACTGGGATCC 
ACCATCCTCT TTGCTCTGCT GCATTTTCAG ATCATCCCAA ACTTTGCTGA AAAGGATGAG 
AGCAAGATGC CGGAAATATT GGCTATCAAG TCTTGTTTAG AGTCTGTACC AGTAGCCCAA 
GCAATGAAAG GGGAAAAGCT GGCAGCTCAT TTTACAGGTT TTAAAGAAAG TGACAAATTA 
TTTTGGGAGC CATTTGATTC CGATTCATCT GTTGCTGAAA AAATGCTCAA GGATAACTCC 
ATCGTTATTC CAGAGAAAGG TTTCTACTTT GTGTACACCC AGGTGTCCTT CACAGGTCCT 
GACTGCAAAA GGGAACCTCT CCATCTTACC CATACAGTCA ACGTAGTAAG GGAGTCATAT 
CCAACTGAAG AGCCAATCCT CACTTCTATG AAGACGGTCT GTGAGGTGCA TTCAGGTGCC 
ACTTGGTTTC AGTCCATCTA TGAAGGGGGG ATCTTTAATC TGGAAAAGGG CTCTGTTATC 
TCAACTGAAA CCAAACACAT TGAGTTTCTT GATGCCGACA AAGAAAGTAA AATCTACCTG 
GGCATCCTTG CCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108250.1
CDS	65..742
Translation	MNSVESQMENGLLIVRQERSNRNSTWRFVSICAFLLLLGSTILFALLHFQIIPNFAEKDESKMPEILAIKSCLESVPVAQAMKGEKLAAHFTGFKESDKLFWEPFDSDSSVAEKMLKDNSIVIPEKGFYFVYTQVSFTGPDCKREPLHLTHTVNVVRESYPTEEPILTSMKTVCEVHSGATWFQSIYEGGIFNLEKGSVISTETKHIEFLDADKESKIYLGILAV

Target ORF information:

RefSeq Version	NM_001114778.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis tumor necrosis factor L homeolog (tnf.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114778.1

ORF Insert Sequence:

ATGAATAGTG TAGAGTCACA GATGGAGAAT GGGCTCCTAA TTGTCAGGCA GGAAAGAAGC 
AACAGGAATT CCACCTGGCG CTTTGTCAGT ATCTGTGCAT TCCTGTTATT ACTGGGATCC 
ACCATCCTCT TTGCTCTGCT GCATTTTCAG ATCATCCCAA ACTTTGCTGA AAAGGATGAG 
AGCAAGATGC CGGAAATATT GGCTATCAAG TCTTGTTTAG AGTCTGTACC AGTAGCCCAA 
GCAATGAAAG GGGAAAAGCT GGCAGCTCAT TTTACAGGTT TTAAAGAAAG TGACAAATTA 
TTTTGGGAGC CATTTGATTC CGATTCATCT GTTGCTGAAA AAATGCTCAA GGATAACTCC 
ATCGTTATTC CAGAGAAAGG TTTCTACTTT GTGTACACCC AGGTGTCCTT CACAGGTCCT 
GACTGCAAAA GGGAACCTCT CCATCTTACC CATACAGTCA ACGTAGTAAG GGAGTCATAT 
CCAACTGAAG AGCCAATCCT CACTTCTATG AAGACGGTCT GTGAGGTGCA TTCAGGTGCC 
ACTTGGTTTC AGTCCATCTA TGAAGGGGGG ATCTTTAATC TGGAAAAGGG CTCTGTTATC 
TCAACTGAAA CCAAACACAT TGAGTTTCTT GATGCCGACA AAGAAAGTAA AATCTACCTG 
GGCATCCTTG CCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tumor necrosis factor-alpha attenuates thyroid hormone-induced apoptosis in vascular endothelial cell line XLgoo established from Xenopus tadpole tails.
Endocrinology149(7)3379-89(2008 Jul)
Mawaribuchi S,Tamura K,Okano S,Takayama S,Yaoita Y,Shiba T,Takamatsu N,Ito M