EDEM1 cDNA ORF clone, Tyto alba(Barn owl)

Gene
Clones
gRNAs

Summary

Gene Symbol	EDEM1
Entrez Gene ID	104364784
Full Name	ER degradation enhancing alpha-mannosidase like protein 1
Gene Type	protein-coding
Organism	Tyto alba(Barn owl)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009971470.1	XP_009969772.1	ER degradation-enhancing alpha-mannosidase-like protein 1

Pathways from BioSystems
Gene Ontology

The following EDEM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDEM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTy05616	XM_009971470.1 Latest version!	Tyto alba ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDEM1 ( XM_009971470.1 ) cDNA ORF clone, Tyto alba(Barn owl) -> XP_009969772.1 Tyto alba ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), mRNA.

Price & Availability↑

CloneID	OTy05616
Clone ID Related Accession (Same CDS sequence)	XM_009971470.1
Accession Version	XM_009971470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Tyto alba(Barn owl)
Product	ER degradation-enhancing alpha-mannosidase-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010013413.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tyto alba Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGTCAGGAG GAGAGTTTGT CTTACTCAAG GTGACTGTAA TGGGAAACTC CTCAGAATTC 
CAGAAAGCAG TGAAGTTGGT GATTGACACG GTTTCATTTG ATAAAGACTC AACGGTTCAA 
GTTTTTGAGG CAACAATCAG GGTTTTGGGA AGCCTGCTTT CTGCCCACAT AATAATTACA 
GATACCAAAC AGCCTTTTGG TGATATGACC ATTAAGGATT ATGACAATGA ACTGTTGCAT 
ATGGCTCATG ACCTGGCAGT GAGACTACTT CCAGCCTTTG AGAACACCAA AACTGGAATT 
CCATATCCTC GGGTCAATCT GAAGAAGGGG GTACCCCCAG ACAGCAATAA TGAAACTTGT 
ACTGCAGGAG CCGGTTCCTT GCTGGTGGAG TTTGGTATCC TTAGCAGGCT GCTGGGCGAT 
TCCACGTTTG AATGGGTGGC CAGGAGAGCT GTCAAAGCCC TCTGGAGTCT CAGGAACAAT 
AACACCGGAC TGCTAGGAAA TGTTGTGAAC ATTCAAACTG GCCATTGGGT TGGAAAGCAA 
AGTGGCTTGG GAGCAGGGTC GGATTCATTC TATGAATACC TTCTGAAGTC TTACATCCTC 
TTTGGAGAAA AGGAAGACTT GGAAATGTTC AATGATGCTT ATCGGAACAT TCAGAACCAC 
TTAAGAAGAG GTCGAGAGGC TTGCAACGAA GGTGAAGGTG ACCCTCCTTT GTATGTCAAT 
GTAAACATGT TCACAGGACA GCTGATGAAC ACGTGGATTG ACTCTTTGCA AGCCTTTTTT 
CCTGGACTAC AGGTATTGAT AGGAGATGTG GAAGATGCCA TTTGTCTCCA TGCTTTTTAT 
TATGCCATAT GGAAACGATA CGGAGCTCTC CCAGAAAGAT ACAACTGGCA ATTGCAAGCA 
CCGGATGTTC CCTTTTATCC ACTGAGGCCA GAGTTAGTAG AGTCCACATA TCTTCTTTAT 
CAGGCCACAA AGAACCCATT TTACCTTCAC GTGGGAATGG ATATTCTGCA GAGTTTGGAA 
AAATATACAA AAGCAAAGTG TGGCTATGCC ACATTACACC ACGTTGTGGA GAAGACAAAG 
GAAGATCGAA TGGAGAGCTT TTTTCTCAGT GAAACATGTA AATACCTGTA CCTGTTGTTT 
GATGAAGAGA ATCCACTGCA TAAATCTGGA AATAAGTATA TGTTTACTAC TGAGGGGCAT 
GTTGTGTCTG TGGATGAACG TTTTCGTAAC TCACTGTGGC AAGACATCCT CCCTGAAGAA 
GAGGACAGAG CAGAAAAAAT TAAACCTAAC GAATTGAAGG CAGTCAACTT CAGCTCTAAC 
TGTAACAGAG TTCCTGATGA GAGGAGATAC TTGCTGCCAT TGAAGAGTAA CTACATGAGA 
CAGATTGATC GAATGGTGGG CTTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009969772.1
CDS	1..1410
Translation	MSGGEFVLLKVTVMGNSSEFQKAVKLVIDTVSFDKDSTVQVFEATIRVLGSLLSAHIIITDTKQPFGDMTIKDYDNELLHMAHDLAVRLLPAFENTKTGIPYPRVNLKKGVPPDSNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWSLRNNNTGLLGNVVNIQTGHWVGKQSGLGAGSDSFYEYLLKSYILFGEKEDLEMFNDAYRNIQNHLRRGREACNEGEGDPPLYVNVNMFTGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVPFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKAKCGYATLHHVVEKTKEDRMESFFLSETCKYLYLLFDEENPLHKSGNKYMFTTEGHVVSVDERFRNSLWQDILPEEEDRAEKIKPNELKAVNFSSNCNRVPDERRYLLPLKSNYMRQIDRMVGLI

Target ORF information:

RefSeq Version	XM_009971470.1
Organism	Tyto alba(Barn owl)
Definition	Tyto alba ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009971470.1

ORF Insert Sequence:

ATGTCAGGAG GAGAGTTTGT CTTACTCAAG GTGACTGTAA TGGGAAACTC CTCAGAATTC 
CAGAAAGCAG TGAAGTTGGT GATTGACACG GTTTCATTTG ATAAAGACTC AACGGTTCAA 
GTTTTTGAGG CAACAATCAG GGTTTTGGGA AGCCTGCTTT CTGCCCACAT AATAATTACA 
GATACCAAAC AGCCTTTTGG TGATATGACC ATTAAGGATT ATGACAATGA ACTGTTGCAT 
ATGGCTCATG ACCTGGCAGT GAGACTACTT CCAGCCTTTG AGAACACCAA AACTGGAATT 
CCATATCCTC GGGTCAATCT GAAGAAGGGG GTACCCCCAG ACAGCAATAA TGAAACTTGT 
ACTGCAGGAG CCGGTTCCTT GCTGGTGGAG TTTGGTATCC TTAGCAGGCT GCTGGGCGAT 
TCCACGTTTG AATGGGTGGC CAGGAGAGCT GTCAAAGCCC TCTGGAGTCT CAGGAACAAT 
AACACCGGAC TGCTAGGAAA TGTTGTGAAC ATTCAAACTG GCCATTGGGT TGGAAAGCAA 
AGTGGCTTGG GAGCAGGGTC GGATTCATTC TATGAATACC TTCTGAAGTC TTACATCCTC 
TTTGGAGAAA AGGAAGACTT GGAAATGTTC AATGATGCTT ATCGGAACAT TCAGAACCAC 
TTAAGAAGAG GTCGAGAGGC TTGCAACGAA GGTGAAGGTG ACCCTCCTTT GTATGTCAAT 
GTAAACATGT TCACAGGACA GCTGATGAAC ACGTGGATTG ACTCTTTGCA AGCCTTTTTT 
CCTGGACTAC AGGTATTGAT AGGAGATGTG GAAGATGCCA TTTGTCTCCA TGCTTTTTAT 
TATGCCATAT GGAAACGATA CGGAGCTCTC CCAGAAAGAT ACAACTGGCA ATTGCAAGCA 
CCGGATGTTC CCTTTTATCC ACTGAGGCCA GAGTTAGTAG AGTCCACATA TCTTCTTTAT 
CAGGCCACAA AGAACCCATT TTACCTTCAC GTGGGAATGG ATATTCTGCA GAGTTTGGAA 
AAATATACAA AAGCAAAGTG TGGCTATGCC ACATTACACC ACGTTGTGGA GAAGACAAAG 
GAAGATCGAA TGGAGAGCTT TTTTCTCAGT GAAACATGTA AATACCTGTA CCTGTTGTTT 
GATGAAGAGA ATCCACTGCA TAAATCTGGA AATAAGTATA TGTTTACTAC TGAGGGGCAT 
GTTGTGTCTG TGGATGAACG TTTTCGTAAC TCACTGTGGC AAGACATCCT CCCTGAAGAA 
GAGGACAGAG CAGAAAAAAT TAAACCTAAC GAATTGAAGG CAGTCAACTT CAGCTCTAAC 
TGTAACAGAG TTCCTGATGA GAGGAGATAC TTGCTGCCAT TGAAGAGTAA CTACATGAGA 
CAGATTGATC GAATGGTGGG CTTGATCTGA