LOC101356953 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101356953
Entrez Gene ID	101356953
Full Name	nitric oxide synthase 3
Synonyms	NOS3
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023727908.1	XP_023583676.1	nitric oxide synthase, endothelial isoform X2
XM_004372734.1	XP_004372791.1	nitric oxide synthase, endothelial isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101356953 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101356953 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTr169103	XM_004372734.1 Latest version!	Trichechus manatus latirostris nitric oxide synthase 3 (LOC101356953), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OTr216661	XM_023727908.1 Latest version!	Trichechus manatus latirostris nitric oxide synthase 3 (LOC101356953), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101356953 ( XM_004372734.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004372791.1 Trichechus manatus latirostris nitric oxide synthase 3 (LOC101356953), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTr169103
Clone ID Related Accession (Same CDS sequence)	XM_004372734.1
Accession Version	XM_004372734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	nitric oxide synthase, endothelial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443956.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCTAGGCCTC CCTGCGGGCT GGGGCTGGGG 
CTGGGCCTCG GGCTATGCAG CAAGCAGGGC CCAGCCTCCC CAGCCTCGGA GCCCAGCCGG 
GCACCAGCAC CAGTGCCCCC ACCTGCGCCC GACCACAGCC GCCCTCCCCT GGCTCCCAAC 
AGCCCCCTAC CAGCCCGGCC CCCAGAAGGA CCCAAGTTCC CTCGTGTGAA GAACTGGGAA 
GTGGGGACCA TCACCTATGA TACCCTGAGT GCCCAGGCAC AGCAGGACGG ACCCTGCACC 
CCAAGACACT GCCTGGGCTC CCTTGTATTT CCACGGAAAC TACAGAGCCA GACATCCCGG 
GGCCCTGCAC CCCCTGAGAG GCTTCTGAAA CAGGCCCGGG ACTTCATCCA CCAGTACTAC 
AGCTCCATCA AGAGGAGCGG CTCCCAGGCC CATGAGCAGC GTCTTCAGGA GGTAGAAGCC 
GAGGTGGTGG CCACAGGCAC CTACCAGCTT CGGGAGAGCG AGCTGGTGTT CGGGGCTAAG 
CAGGCCTGGC GCAATGCTCC CCGATGCGTC GGCCGCATCC AGTGGGGGAA GCTGCAGGTA 
TTTGATGCCC GGGACTGCAG CTCTGCGCAG GAGATGTTCA CCTACATCTG CAACCACATC 
AAGTATGGCA CCAATAGGGG TAACCTTCGC TCTGCCATCA CCGTGTTCCC CCAACGTGTC 
CCTGGCCGCG GTGATTTCCG GATCTGGAAC AGCCAGCTGG TGCGTTATGC TGGCTACAGG 
CAGCAGGATG GCTCCGTGCG TGGGGACCCT GCCAACGTGG AGATCACTGA GCTCTGCATC 
CAGCACGGCT GGACCCCAGG AAATGGCCGA TTTGATGTGC TGCCCCTGCT GCTCCAGGCC 
CCAGATGAGC CCCCGGAGGT CTTCCCTTTG CCCCCTGAAC TGGTCCTTGA GGTGCCCCTG 
GAGCACCCCA CGCTGGAGTG GTTTGCAGCC CTGGGCCTGC GTTGGTATGC CCTCCCAGCG 
GTGTCCAACA TGCTGCTGGA AATCGGGGGC CTGGAGTTCA CTGCGGCTCC TTTCAGCGGC 
TGGTACATGA GCACTGAGAT TGGCACTCGG AACCTGTGTG ATCCCCACCG CTACAACATT 
CTAGAGGATG TGGCCGTCTG CATGGACCTA GACACACGGA CCACCTCGTC CCTGTGGAAA 
GACAAGGCAG CTGTGGAAAT TAACTTGGCT GTGCTGCACA GTTACCAGCT GGCCAAAGTG 
ACCATTGTGG ACCACCACGC TGCCACAGCC TCCTTCATGA AGCACCTGGA GAATGAGCAG 
AAGGCGAGGG GAGGGTGCCC TGCCGACTGG GCCTGGATCG TGCCCCCCAT CTCTGGCAGC 
CTCACACCTG TCTTCCATCA AGAGATGGTC AACTATTTCC TGTCCCCCGC CTTCCACTAC 
CAGCCAGATC CCTGGAAGGG AAGTGCAGCC AAGGGCACAG GTATCACCAG GAAGAAGACC 
TTTAAGGAAG TGGCCAATGC AGTCAAGATC TCTGCCTCGC TCATGGGCAC TGTGATGGCA 
AAGCGAGTGA AGGCGACAAT CCTGTATGGC TCTGAGACTG GCCGGGCCCA GAGCTACGCA 
CAGCAGCTGG GGAGGCTCTT CCGGAAGGCT TTCGATCCCC GGGTCCTGTG CATGGATGAG 
TATGATGTGG TGTCCCTTGA GCACGAGACA CTGGTGGTTG TGGTGACCAG CACATTTGGG 
AACGGTGATC CACCCGAGAA CGGCGAGAGC TTTGCAGCCG CCCTGATGGA AATGACCAGC 
CCCTACAACA GCTCCCCTCG ACCCGAACAG CACAAGAGCT ACAAAATGCG CTTCAACAGT 
GTCTCTTGCT CAGACCCCCT GGTCTCCTCC TGGCGGCGGA AAAGGAAGGA GTCTAGTAAC 
ACAGACAGTG CGGGGGCGCT GGGCACCCTC AGGTTCTGTG TGTTCGGGCT GGGCTCCCGG 
GCATATCCCC ACTTCTGCGC CTTCGCGCGC GCAGTGGACA CGCGGCTGGA GGAGCTGGGC 
GGAGAGCGGC TGCTGCAGCT GGGCCAGGGG GATGAGCTGT GCGGCCAGGA GGAAGCCTTC 
CGTGGCTGGG CCCAGGCCGC CTTCCAGGCC GCCTGTGAGA CTTTCTGTGT GGGAGAAGAC 
GCCAAGGCTG CTGCCCGGGA TATATTCAGT CCCAAACGGA GCTGGAAGCG CCAGAGATAC 
CGGCTGAGTC CCCAGGCCAA GGGCCTCCAG CTGCTGCCAG GCCTGATCCA CGTGCACAGG 
CGGAAAATGT TCCAAGCTAC TGTCCTCTCA GTGGAAAACC TGCAAAGCAG CAAGTCCACC 
CGGGCCACCA TCCTGGTGCG CCTAGACATT GGAGGGCAGC AGGGGCTGCA GTATCAGCCA 
GGGGACCACA TTGGGGTCTG CCCGCCCAAC CGGCCTGGCC TGGTGGAGGC GCTGCTGAGC 
CGCGTGGAAG ACCCGCCGCC GCCCACCGAG CCCATTGCAG TGGAGCAGCT GGAGAAGGGC 
AGCCCTGGTG GCCCGCCCCC CGGCTGGGTA CGGGACCCCA GGCTGCCCCC ATGCACACTG 
CGCCAGGCTC TGACCTACTT CCTGGACATC ACATCCCCAC CCAGCCCTCA ACTCCTTCGA 
CTGCTCAGCA CCCTCGCTGA AGAGCCCAGT GAACAGCAGG AGCTGGAAAC CCTCAGCCAG 
GACCCCCGGC GCTACGAGGA GTGGAAGTGG TTCCGTTGCC CCACGCTTCT GGAAGTGCTG 
GAGCAGTTTC CGTCGGTAGC ACTGCCGGCC CCCCTGCTCC TCACCCAGCT GCCTCTGCTC 
CAGCCACGGT ACTACTCCGT CAGCTCAGCA CCCAGTGCCT CCCCAGGAGA GATCCACCTC 
ACAGTAGCTG TGCTGGCATA CCGGACCCAG GATGGCCTGG GCCCACTGCA CTATGGTGTC 
TGCTCCACGT GGCTGAGCCA GCTCAAGGTT GGAGACCCTG TGCCCTGCTT CATCAGAGGT 
GCTCCCTCCT TCCGGCTGCC GCCTGATCCC AGCTTGCCCT GCATCCTCGT GGGACCTGGC 
ACGGGCATTG CCCCCTTCCG GGGATTCTGG CAGGAGAGGC TGCACGACAT TGAAAGCAAA 
GGGCTGCAGC CCGCCCCCAT GACCTTGGTG TTTGGCTGCC GATGCTCCCA GCTCGACCAC 
CTCTACCGTG AGGAGGTGCA AGACGCCCAG CAGCGCGGGG TGTTTGACCG TGTTCTCACC 
GCCTTCTCCC GGGAACCCGA CAGCCCCAAG ACTTACGTGC AGGACCTCCT GCGGACTGAG 
CTGGCTGCTG AGGTGCACCG CGTGCTGTGC CTCGAGCGGG GCCACATGTT CGTCTGTGGC 
GATGTCACCA TGGCGACCAG CGTCCTGCAA ACCGTGCAGC GCATCCTAGC GGCAGAGGGC 
GACATGGAGC TGGACGAGGC CGGAGACTTC ATCGGCGTGC TGCGGGATCA GCAACGCTAC 
CACGAGGACA TTTTCGGGCT CACCCTGCGC ACCCAGGAGG TGACAAGCCG CATACGCACC 
CAGAGCTTTT CCCTACAGGA GCGGCAGCTG CGGGGTGCAG TGCCCTGGGC GTTCGACCCG 
CCCGGTGCAA ACACCCCTGG CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004372791.1
CDS	77..3703
Translation	MGNLKSVGQEPRPPCGLGLGLGLGLCSKQGPASPASEPSRAPAPVPPPAPDHSRPPLAPNSPLPARPPEGPKFPRVKNWEVGTITYDTLSAQAQQDGPCTPRHCLGSLVFPRKLQSQTSRGPAPPERLLKQARDFIHQYYSSIKRSGSQAHEQRLQEVEAEVVATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCSSAQEMFTYICNHIKYGTNRGNLRSAITVFPQRVPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEPPEVFPLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFTAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFHYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVVVVTSTFGNGDPPENGESFAAALMEMTSPYNSSPRPEQHKSYKMRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSPQAKGLQLLPGLIHVHRRKMFQATVLSVENLQSSKSTRATILVRLDIGGQQGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPPPTEPIAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTYFLDITSPPSPQLLRLLSTLAEEPSEQQELETLSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSASPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKVGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPAPMTLVFGCRCSQLDHLYREEVQDAQQRGVFDRVLTAFSREPDSPKTYVQDLLRTELAAEVHRVLCLERGHMFVCGDVTMATSVLQTVQRILAAEGDMELDEAGDFIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGANTPGP

Target ORF information:

RefSeq Version	XM_004372734.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris nitric oxide synthase 3 (LOC101356953), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004372734.1

ORF Insert Sequence:

ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCTAGGCCTC CCTGCGGGCT GGGGCTGGGG 
CTGGGCCTCG GGCTATGCAG CAAGCAGGGC CCAGCCTCCC CAGCCTCGGA GCCCAGCCGG 
GCACCAGCAC CAGTGCCCCC ACCTGCGCCC GACCACAGCC GCCCTCCCCT GGCTCCCAAC 
AGCCCCCTAC CAGCCCGGCC CCCAGAAGGA CCCAAGTTCC CTCGTGTGAA GAACTGGGAA 
GTGGGGACCA TCACCTATGA TACCCTGAGT GCCCAGGCAC AGCAGGACGG ACCCTGCACC 
CCAAGACACT GCCTGGGCTC CCTTGTATTT CCACGGAAAC TACAGAGCCA GACATCCCGG 
GGCCCTGCAC CCCCTGAGAG GCTTCTGAAA CAGGCCCGGG ACTTCATCCA CCAGTACTAC 
AGCTCCATCA AGAGGAGCGG CTCCCAGGCC CATGAGCAGC GTCTTCAGGA GGTAGAAGCC 
GAGGTGGTGG CCACAGGCAC CTACCAGCTT CGGGAGAGCG AGCTGGTGTT CGGGGCTAAG 
CAGGCCTGGC GCAATGCTCC CCGATGCGTC GGCCGCATCC AGTGGGGGAA GCTGCAGGTA 
TTTGATGCCC GGGACTGCAG CTCTGCGCAG GAGATGTTCA CCTACATCTG CAACCACATC 
AAGTATGGCA CCAATAGGGG TAACCTTCGC TCTGCCATCA CCGTGTTCCC CCAACGTGTC 
CCTGGCCGCG GTGATTTCCG GATCTGGAAC AGCCAGCTGG TGCGTTATGC TGGCTACAGG 
CAGCAGGATG GCTCCGTGCG TGGGGACCCT GCCAACGTGG AGATCACTGA GCTCTGCATC 
CAGCACGGCT GGACCCCAGG AAATGGCCGA TTTGATGTGC TGCCCCTGCT GCTCCAGGCC 
CCAGATGAGC CCCCGGAGGT CTTCCCTTTG CCCCCTGAAC TGGTCCTTGA GGTGCCCCTG 
GAGCACCCCA CGCTGGAGTG GTTTGCAGCC CTGGGCCTGC GTTGGTATGC CCTCCCAGCG 
GTGTCCAACA TGCTGCTGGA AATCGGGGGC CTGGAGTTCA CTGCGGCTCC TTTCAGCGGC 
TGGTACATGA GCACTGAGAT TGGCACTCGG AACCTGTGTG ATCCCCACCG CTACAACATT 
CTAGAGGATG TGGCCGTCTG CATGGACCTA GACACACGGA CCACCTCGTC CCTGTGGAAA 
GACAAGGCAG CTGTGGAAAT TAACTTGGCT GTGCTGCACA GTTACCAGCT GGCCAAAGTG 
ACCATTGTGG ACCACCACGC TGCCACAGCC TCCTTCATGA AGCACCTGGA GAATGAGCAG 
AAGGCGAGGG GAGGGTGCCC TGCCGACTGG GCCTGGATCG TGCCCCCCAT CTCTGGCAGC 
CTCACACCTG TCTTCCATCA AGAGATGGTC AACTATTTCC TGTCCCCCGC CTTCCACTAC 
CAGCCAGATC CCTGGAAGGG AAGTGCAGCC AAGGGCACAG GTATCACCAG GAAGAAGACC 
TTTAAGGAAG TGGCCAATGC AGTCAAGATC TCTGCCTCGC TCATGGGCAC TGTGATGGCA 
AAGCGAGTGA AGGCGACAAT CCTGTATGGC TCTGAGACTG GCCGGGCCCA GAGCTACGCA 
CAGCAGCTGG GGAGGCTCTT CCGGAAGGCT TTCGATCCCC GGGTCCTGTG CATGGATGAG 
TATGATGTGG TGTCCCTTGA GCACGAGACA CTGGTGGTTG TGGTGACCAG CACATTTGGG 
AACGGTGATC CACCCGAGAA CGGCGAGAGC TTTGCAGCCG CCCTGATGGA AATGACCAGC 
CCCTACAACA GCTCCCCTCG ACCCGAACAG CACAAGAGCT ACAAAATGCG CTTCAACAGT 
GTCTCTTGCT CAGACCCCCT GGTCTCCTCC TGGCGGCGGA AAAGGAAGGA GTCTAGTAAC 
ACAGACAGTG CGGGGGCGCT GGGCACCCTC AGGTTCTGTG TGTTCGGGCT GGGCTCCCGG 
GCATATCCCC ACTTCTGCGC CTTCGCGCGC GCAGTGGACA CGCGGCTGGA GGAGCTGGGC 
GGAGAGCGGC TGCTGCAGCT GGGCCAGGGG GATGAGCTGT GCGGCCAGGA GGAAGCCTTC 
CGTGGCTGGG CCCAGGCCGC CTTCCAGGCC GCCTGTGAGA CTTTCTGTGT GGGAGAAGAC 
GCCAAGGCTG CTGCCCGGGA TATATTCAGT CCCAAACGGA GCTGGAAGCG CCAGAGATAC 
CGGCTGAGTC CCCAGGCCAA GGGCCTCCAG CTGCTGCCAG GCCTGATCCA CGTGCACAGG 
CGGAAAATGT TCCAAGCTAC TGTCCTCTCA GTGGAAAACC TGCAAAGCAG CAAGTCCACC 
CGGGCCACCA TCCTGGTGCG CCTAGACATT GGAGGGCAGC AGGGGCTGCA GTATCAGCCA 
GGGGACCACA TTGGGGTCTG CCCGCCCAAC CGGCCTGGCC TGGTGGAGGC GCTGCTGAGC 
CGCGTGGAAG ACCCGCCGCC GCCCACCGAG CCCATTGCAG TGGAGCAGCT GGAGAAGGGC 
AGCCCTGGTG GCCCGCCCCC CGGCTGGGTA CGGGACCCCA GGCTGCCCCC ATGCACACTG 
CGCCAGGCTC TGACCTACTT CCTGGACATC ACATCCCCAC CCAGCCCTCA ACTCCTTCGA 
CTGCTCAGCA CCCTCGCTGA AGAGCCCAGT GAACAGCAGG AGCTGGAAAC CCTCAGCCAG 
GACCCCCGGC GCTACGAGGA GTGGAAGTGG TTCCGTTGCC CCACGCTTCT GGAAGTGCTG 
GAGCAGTTTC CGTCGGTAGC ACTGCCGGCC CCCCTGCTCC TCACCCAGCT GCCTCTGCTC 
CAGCCACGGT ACTACTCCGT CAGCTCAGCA CCCAGTGCCT CCCCAGGAGA GATCCACCTC 
ACAGTAGCTG TGCTGGCATA CCGGACCCAG GATGGCCTGG GCCCACTGCA CTATGGTGTC 
TGCTCCACGT GGCTGAGCCA GCTCAAGGTT GGAGACCCTG TGCCCTGCTT CATCAGAGGT 
GCTCCCTCCT TCCGGCTGCC GCCTGATCCC AGCTTGCCCT GCATCCTCGT GGGACCTGGC 
ACGGGCATTG CCCCCTTCCG GGGATTCTGG CAGGAGAGGC TGCACGACAT TGAAAGCAAA 
GGGCTGCAGC CCGCCCCCAT GACCTTGGTG TTTGGCTGCC GATGCTCCCA GCTCGACCAC 
CTCTACCGTG AGGAGGTGCA AGACGCCCAG CAGCGCGGGG TGTTTGACCG TGTTCTCACC 
GCCTTCTCCC GGGAACCCGA CAGCCCCAAG ACTTACGTGC AGGACCTCCT GCGGACTGAG 
CTGGCTGCTG AGGTGCACCG CGTGCTGTGC CTCGAGCGGG GCCACATGTT CGTCTGTGGC 
GATGTCACCA TGGCGACCAG CGTCCTGCAA ACCGTGCAGC GCATCCTAGC GGCAGAGGGC 
GACATGGAGC TGGACGAGGC CGGAGACTTC ATCGGCGTGC TGCGGGATCA GCAACGCTAC 
CACGAGGACA TTTTCGGGCT CACCCTGCGC ACCCAGGAGG TGACAAGCCG CATACGCACC 
CAGAGCTTTT CCCTACAGGA GCGGCAGCTG CGGGGTGCAG TGCCCTGGGC GTTCGACCCG 
CCCGGTGCAA ACACCCCTGG CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101356953 ( XM_023727908.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023583676.1 Trichechus manatus latirostris nitric oxide synthase 3 (LOC101356953), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTr216661
Clone ID Related Accession (Same CDS sequence)	XM_023727908.1
Accession Version	XM_023727908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	nitric oxide synthase, endothelial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443956.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCTAGGCCTC CCTGCGGGCT GGGGCTGGGG 
CTGGGCCTCG GGCTATGCAG CAAGCAGGGC CCAGCCTCCC CAGCCTCGGA GCCCAGCCGG 
GCACCAGCAC CAGTGCCCCC ACCTGCGCCC GACCACAGCC GCCCTCCCCT GGCTCCCAAC 
AGCCCCCTAC CAGCCCGGCC CCCAGAAGGA CCCAAGTTCC CTCGTGTGAA GAACTGGGAA 
GTGGGGACCA TCACCTATGA TACCCTGAGT GCCCAGGCAC AGCAGGACGG ACCCTGCACC 
CCAAGACACT GCCTGGGCTC CCTTGTATTT CCACGGAAAC TACAGAGCCA GACATCCCGG 
GGCCCTGCAC CCCCTGAGAG GCTTCTGAAA CAGGCCCGGG ACTTCATCCA CCAGTACTAC 
AGCTCCATCA AGAGGAGCGG CTCCCAGGCC CATGAGCAGC GTCTTCAGGA GGTAGAAGCC 
GAGGTGGTGG CCACAGGCAC CTACCAGCTT CGGGAGAGCG AGCTGGTGTT CGGGGCTAAG 
CAGGCCTGGC GCAATGCTCC CCGATGCGTC GGCCGCATCC AGTGGGGGAA GCTGCAGGTA 
TTTGATGCCC GGGACTGCAG CTCTGCGCAG GAGATGTTCA CCTACATCTG CAACCACATC 
AAGTATGGCA CCAATAGGGG TAACCTTCGC TCTGCCATCA CCGTGTTCCC CCAACGTGTC 
CCTGGCCGCG GTGATTTCCG GATCTGGAAC AGCCAGCTGG TGCGTTATGC TGGCTACAGG 
CAGCAGGATG GCTCCGTGCG TGGGGACCCT GCCAACGTGG AGATCACTGA GCTCTGCATC 
CAGCACGGCT GGACCCCAGG AAATGGCCGA TTTGATGTGC TGCCCCTGCT GCTCCAGGCC 
CCAGATGAGC CCCCGGAGGT CTTCCCTTTG CCCCCTGAAC TGGTCCTTGA GGTGCCCCTG 
GAGCACCCCA CGCTGGAGTG GTTTGCAGCC CTGGGCCTGC GTTGGTATGC CCTCCCAGCG 
GTGTCCAACA TGCTGCTGGA AATCGGGGGC CTGGAGTTCA CTGCGGCTCC TTTCAGCGGC 
TGGTACATGA GCACTGAGAT TGGCACTCGG AACCTGTGTG ATCCCCACCG CTACAACATT 
CTAGAGGATG TGGCCGTCTG CATGGACCTA GACACACGGA CCACCTCGTC CCTGTGGAAA 
GACAAGGCAG CTGTGGAAAT TAACTTGGCT GTGCTGCACA GTTACCAGCT GGCCAAAGTG 
ACCATTGTGG ACCACCACGC TGCCACAGCC TCCTTCATGA AGCACCTGGA GAATGAGCAG 
AAGGCGAGGG GAGGGTGCCC TGCCGACTGG GCCTGGATCG TGCCCCCCAT CTCTGGCAGC 
CTCACACCTG TCTTCCATCA AGAGATGGTC AACTATTTCC TGTCCCCCGC CTTCCACTAC 
CAGCCAGATC CCTGGAAGGG AAGTGCAGCC AAGGGCACAG GTATCACCAG GAAGAAGACC 
TTTAAGGAAG TGGCCAATGC AGTCAAGATC TCTGCCTCGC TCATGGGCAC TGTGATGGCA 
AAGCGAGTGA AGGCGACAAT CCTGTATGGC TCTGAGACTG GCCGGGCCCA GAGCTACGCA 
CAGCAGCTGG GGAGGCTCTT CCGGAAGGCT TTCGATCCCC GGGTCCTGTG CATGGATGAG 
TATGATGTGG TGTCCCTTGA GCACGAGACA CTGGTGGTTG TGGTGACCAG CACATTTGGG 
AACGGTGATC CACCCGAGAA CGGCGAGAGC TTTGCAGCCG CCCTGATGGA AATGACCAGC 
CCCTACAACA GCTCCCCTCG ACCCGAACAG CACAAGAGCT ACAAAATGCG CTTCAACAGT 
GTCTCTTGCT CAGACCCCCT GGTCTCCTCC TGGCGGCGGA AAAGGAAGGA GTCTAGTAAC 
ACAGACAGTG CGGGGGCGCT GGGCACCCTC AGGTTCTGTG TGTTCGGGCT GGGCTCCCGG 
GCATATCCCC ACTTCTGCGC CTTCGCGCGC GCAGTGGACA CGCGGCTGGA GGAGCTGGGC 
GGAGAGCGGC TGCTGCAGCT GGGCCAGGGG GATGAGCTGT GCGGCCAGGA GGAAGCCTTC 
CGTGGCTGGG CCCAGGCCGC CTTCCAGGCC GCCTGTGAGA CTTTCTGTGT GGGAGAAGAC 
GCCAAGGCTG CTGCCCGGGA TATATTCAGT CCCAAACGGA GCTGGAAGCG CCAGAGATAC 
CGGCTGAGTC CCCAGGCCAA GGGCCTCCAG CTGCTGCCAG GCCTGATCCA CGTGCACAGG 
CGGAAAATGT TCCAAGCTAC TGTCCTCTCA GTGGAAAACC TGCAAAGCAG CAAGTCCACC 
CGGGCCACCA TCCTGGTGCG CCTAGACATT GGAGGGCAGC AGGGGCTGCA GTATCAGCCA 
GGGGACCACA TTGGGGTCTG CCCGCCCAAC CGGCCTGGCC TGGTGGAGGC GCTGCTGAGC 
CGCGTGGAAG ACCCGCCGCC GCCCACCGAG CCCATTGCAG TGGAGCAGCT GGAGAAGGGC 
AGCCCTGGTG GCCCGCCCCC CGGCTGGGTA CGGGACCCCA GGCTGCCCCC ATGCACACTG 
CGCCAGGCTC TGACCTACTT CCTGGACATC ACATCCCCAC CCAGCCCTCA ACTCCTTCGA 
CTGCTCAGCA CCCTCGCTGA AGAGCCCAGT GAACAGCAGG AGCTGGAAAC CCTCAGCCAG 
GACCCCCGGC GCTACGAGGA GTGGAAGTGG TTCCGTTGCC CCACGCTTCT GGAAGTGCTG 
GAGCAGTTTC CGTCGGTAGC ACTGCCGGCC CCCCTGCTCC TCACCCAGCT GCCTCTGCTC 
CAGCCACGGT ACTACTCCGT CAGCTCAGCA CCCAGTGCCT CCCCAGGAGA GATCCACCTC 
ACAGTAGCTG TGCTGGCATA CCGGACCCAG GATGGCCTGG GCCCACTGCA CTATGGTGTC 
TGCTCCACGT GGCTGAGCCA GCTCAAGGTT GGAGACCCTG TGCCCTGCTT CATCAGAGGT 
GCTCCCTCCT TCCGGCTGCC GCCTGATCCC AGCTTGCCCT GCATCCTCGT GGGACCTGGC 
ACGGGCATTG CCCCCTTCCG GGGATTCTGG CAGGAGAGGC TGCACGACAT TGAAAGCAAA 
GGGCTGCAGC CCGCCCCCAT GACCTTGGTG TTTGGCTGCC GATGCTCCCA GCTCGACCAC 
CTCTACCGTG AGGAGGTGCA AGACGCCCAG CAGCGCGGGG TGTTTGACCG TGTTCTCACC 
GCCTTCTCCC GGGAACCCGA CAGCCCCAAG ACTTACGTGC AGGACCTCCT GCGGACTGAG 
CTGGCTGCTG AGGTGCACCG CGTGCTGTGC CTCGAGCGGG GCCACATGTT CGTCTGTGGC 
GATGTCACCA TGGCGACCAG CGTCCTGCAA ACCGTGCAGC GCATCCTAGC GGCAGAGGGC 
GACATGGAGC TGGACGAGGC CGGAGACTTC ATCGGCGTGC TGCGGGGCCC TCCTTCCCCG 
CAGGATCAGC AACGCTACCA CGAGGACATT TTCGGGCTCA CCCTGCGCAC CCAGGAGGTG 
ACAAGCCGCA TACGCACCCA GAGCTTTTCC CTACAGGAGC GGCAGCTGCG GGGTGCAGTG 
CCCTGGGCGT TCGACCCGCC CGGTGCAAAC ACCCCTGGCC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023583676.1
CDS	77..3721
Translation	MGNLKSVGQEPRPPCGLGLGLGLGLCSKQGPASPASEPSRAPAPVPPPAPDHSRPPLAPNSPLPARPPEGPKFPRVKNWEVGTITYDTLSAQAQQDGPCTPRHCLGSLVFPRKLQSQTSRGPAPPERLLKQARDFIHQYYSSIKRSGSQAHEQRLQEVEAEVVATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCSSAQEMFTYICNHIKYGTNRGNLRSAITVFPQRVPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEPPEVFPLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFTAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFHYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVVVVTSTFGNGDPPENGESFAAALMEMTSPYNSSPRPEQHKSYKMRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSPQAKGLQLLPGLIHVHRRKMFQATVLSVENLQSSKSTRATILVRLDIGGQQGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPPPTEPIAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTYFLDITSPPSPQLLRLLSTLAEEPSEQQELETLSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSASPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKVGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPAPMTLVFGCRCSQLDHLYREEVQDAQQRGVFDRVLTAFSREPDSPKTYVQDLLRTELAAEVHRVLCLERGHMFVCGDVTMATSVLQTVQRILAAEGDMELDEAGDFIGVLRGPPSPQDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGANTPGP

Target ORF information:

RefSeq Version	XM_023727908.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris nitric oxide synthase 3 (LOC101356953), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023727908.1

ORF Insert Sequence:

ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCTAGGCCTC CCTGCGGGCT GGGGCTGGGG 
CTGGGCCTCG GGCTATGCAG CAAGCAGGGC CCAGCCTCCC CAGCCTCGGA GCCCAGCCGG 
GCACCAGCAC CAGTGCCCCC ACCTGCGCCC GACCACAGCC GCCCTCCCCT GGCTCCCAAC 
AGCCCCCTAC CAGCCCGGCC CCCAGAAGGA CCCAAGTTCC CTCGTGTGAA GAACTGGGAA 
GTGGGGACCA TCACCTATGA TACCCTGAGT GCCCAGGCAC AGCAGGACGG ACCCTGCACC 
CCAAGACACT GCCTGGGCTC CCTTGTATTT CCACGGAAAC TACAGAGCCA GACATCCCGG 
GGCCCTGCAC CCCCTGAGAG GCTTCTGAAA CAGGCCCGGG ACTTCATCCA CCAGTACTAC 
AGCTCCATCA AGAGGAGCGG CTCCCAGGCC CATGAGCAGC GTCTTCAGGA GGTAGAAGCC 
GAGGTGGTGG CCACAGGCAC CTACCAGCTT CGGGAGAGCG AGCTGGTGTT CGGGGCTAAG 
CAGGCCTGGC GCAATGCTCC CCGATGCGTC GGCCGCATCC AGTGGGGGAA GCTGCAGGTA 
TTTGATGCCC GGGACTGCAG CTCTGCGCAG GAGATGTTCA CCTACATCTG CAACCACATC 
AAGTATGGCA CCAATAGGGG TAACCTTCGC TCTGCCATCA CCGTGTTCCC CCAACGTGTC 
CCTGGCCGCG GTGATTTCCG GATCTGGAAC AGCCAGCTGG TGCGTTATGC TGGCTACAGG 
CAGCAGGATG GCTCCGTGCG TGGGGACCCT GCCAACGTGG AGATCACTGA GCTCTGCATC 
CAGCACGGCT GGACCCCAGG AAATGGCCGA TTTGATGTGC TGCCCCTGCT GCTCCAGGCC 
CCAGATGAGC CCCCGGAGGT CTTCCCTTTG CCCCCTGAAC TGGTCCTTGA GGTGCCCCTG 
GAGCACCCCA CGCTGGAGTG GTTTGCAGCC CTGGGCCTGC GTTGGTATGC CCTCCCAGCG 
GTGTCCAACA TGCTGCTGGA AATCGGGGGC CTGGAGTTCA CTGCGGCTCC TTTCAGCGGC 
TGGTACATGA GCACTGAGAT TGGCACTCGG AACCTGTGTG ATCCCCACCG CTACAACATT 
CTAGAGGATG TGGCCGTCTG CATGGACCTA GACACACGGA CCACCTCGTC CCTGTGGAAA 
GACAAGGCAG CTGTGGAAAT TAACTTGGCT GTGCTGCACA GTTACCAGCT GGCCAAAGTG 
ACCATTGTGG ACCACCACGC TGCCACAGCC TCCTTCATGA AGCACCTGGA GAATGAGCAG 
AAGGCGAGGG GAGGGTGCCC TGCCGACTGG GCCTGGATCG TGCCCCCCAT CTCTGGCAGC 
CTCACACCTG TCTTCCATCA AGAGATGGTC AACTATTTCC TGTCCCCCGC CTTCCACTAC 
CAGCCAGATC CCTGGAAGGG AAGTGCAGCC AAGGGCACAG GTATCACCAG GAAGAAGACC 
TTTAAGGAAG TGGCCAATGC AGTCAAGATC TCTGCCTCGC TCATGGGCAC TGTGATGGCA 
AAGCGAGTGA AGGCGACAAT CCTGTATGGC TCTGAGACTG GCCGGGCCCA GAGCTACGCA 
CAGCAGCTGG GGAGGCTCTT CCGGAAGGCT TTCGATCCCC GGGTCCTGTG CATGGATGAG 
TATGATGTGG TGTCCCTTGA GCACGAGACA CTGGTGGTTG TGGTGACCAG CACATTTGGG 
AACGGTGATC CACCCGAGAA CGGCGAGAGC TTTGCAGCCG CCCTGATGGA AATGACCAGC 
CCCTACAACA GCTCCCCTCG ACCCGAACAG CACAAGAGCT ACAAAATGCG CTTCAACAGT 
GTCTCTTGCT CAGACCCCCT GGTCTCCTCC TGGCGGCGGA AAAGGAAGGA GTCTAGTAAC 
ACAGACAGTG CGGGGGCGCT GGGCACCCTC AGGTTCTGTG TGTTCGGGCT GGGCTCCCGG 
GCATATCCCC ACTTCTGCGC CTTCGCGCGC GCAGTGGACA CGCGGCTGGA GGAGCTGGGC 
GGAGAGCGGC TGCTGCAGCT GGGCCAGGGG GATGAGCTGT GCGGCCAGGA GGAAGCCTTC 
CGTGGCTGGG CCCAGGCCGC CTTCCAGGCC GCCTGTGAGA CTTTCTGTGT GGGAGAAGAC 
GCCAAGGCTG CTGCCCGGGA TATATTCAGT CCCAAACGGA GCTGGAAGCG CCAGAGATAC 
CGGCTGAGTC CCCAGGCCAA GGGCCTCCAG CTGCTGCCAG GCCTGATCCA CGTGCACAGG 
CGGAAAATGT TCCAAGCTAC TGTCCTCTCA GTGGAAAACC TGCAAAGCAG CAAGTCCACC 
CGGGCCACCA TCCTGGTGCG CCTAGACATT GGAGGGCAGC AGGGGCTGCA GTATCAGCCA 
GGGGACCACA TTGGGGTCTG CCCGCCCAAC CGGCCTGGCC TGGTGGAGGC GCTGCTGAGC 
CGCGTGGAAG ACCCGCCGCC GCCCACCGAG CCCATTGCAG TGGAGCAGCT GGAGAAGGGC 
AGCCCTGGTG GCCCGCCCCC CGGCTGGGTA CGGGACCCCA GGCTGCCCCC ATGCACACTG 
CGCCAGGCTC TGACCTACTT CCTGGACATC ACATCCCCAC CCAGCCCTCA ACTCCTTCGA 
CTGCTCAGCA CCCTCGCTGA AGAGCCCAGT GAACAGCAGG AGCTGGAAAC CCTCAGCCAG 
GACCCCCGGC GCTACGAGGA GTGGAAGTGG TTCCGTTGCC CCACGCTTCT GGAAGTGCTG 
GAGCAGTTTC CGTCGGTAGC ACTGCCGGCC CCCCTGCTCC TCACCCAGCT GCCTCTGCTC 
CAGCCACGGT ACTACTCCGT CAGCTCAGCA CCCAGTGCCT CCCCAGGAGA GATCCACCTC 
ACAGTAGCTG TGCTGGCATA CCGGACCCAG GATGGCCTGG GCCCACTGCA CTATGGTGTC 
TGCTCCACGT GGCTGAGCCA GCTCAAGGTT GGAGACCCTG TGCCCTGCTT CATCAGAGGT 
GCTCCCTCCT TCCGGCTGCC GCCTGATCCC AGCTTGCCCT GCATCCTCGT GGGACCTGGC 
ACGGGCATTG CCCCCTTCCG GGGATTCTGG CAGGAGAGGC TGCACGACAT TGAAAGCAAA 
GGGCTGCAGC CCGCCCCCAT GACCTTGGTG TTTGGCTGCC GATGCTCCCA GCTCGACCAC 
CTCTACCGTG AGGAGGTGCA AGACGCCCAG CAGCGCGGGG TGTTTGACCG TGTTCTCACC 
GCCTTCTCCC GGGAACCCGA CAGCCCCAAG ACTTACGTGC AGGACCTCCT GCGGACTGAG 
CTGGCTGCTG AGGTGCACCG CGTGCTGTGC CTCGAGCGGG GCCACATGTT CGTCTGTGGC 
GATGTCACCA TGGCGACCAG CGTCCTGCAA ACCGTGCAGC GCATCCTAGC GGCAGAGGGC 
GACATGGAGC TGGACGAGGC CGGAGACTTC ATCGGCGTGC TGCGGGGCCC TCCTTCCCCG 
CAGGATCAGC AACGCTACCA CGAGGACATT TTCGGGCTCA CCCTGCGCAC CCAGGAGGTG 
ACAAGCCGCA TACGCACCCA GAGCTTTTCC CTACAGGAGC GGCAGCTGCG GGGTGCAGTG 
CCCTGGGCGT TCGACCCGCC CGGTGCAAAC ACCCCTGGCC CCTGA