LOC101354974 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101354974
Entrez Gene ID	101354974
Full Name	erythrocyte membrane protein band 4.1 like 2
Synonyms	EPB41L2
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012554911.1	XP_012410365.1	band 4.1-like protein 2
XM_023727906.1	XP_023583674.1	band 4.1-like protein 2

Pathways from BioSystems
Gene Ontology

The following LOC101354974 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101354974 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTr164925	XM_012554911.1 Latest version!	Trichechus manatus latirostris erythrocyte membrane protein band 4.1-like 2 (LOC101354974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OTr214139	XM_023727906.1 Latest version!	Trichechus manatus latirostris erythrocyte membrane protein band 4.1 like 2 (LOC101354974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101354974 ( XM_012554911.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012410365.1 Trichechus manatus latirostris erythrocyte membrane protein band 4.1-like 2 (LOC101354974), mRNA.

Price & Availability↑

CloneID	OTr164925
Clone ID Related Accession (Same CDS sequence)	XM_012554911.1
Accession Version	XM_012554911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	band 4.1-like protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443938.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGACCACTG AAGTAGGCTC CGTGTCTGAA GTGAAAAAGG AATCTGACCA GTTAGGAGCA 
GATGCAACCA AGGAGAAACC TGAAGAAGTA GCAGAAAATC AGCAGAATCA ATCATCCGAC 
CCAGAGGAGG AAAAAGGTCT CCAGTCACCT CCTGCAGCTG AAAGCCAAAG TAGTCCACGC 
CGCCAGAAGA GAGAGAAGGA TCCATCTGAG AGCAGGGGTA TTTCTCGATT CATACCCCCA 
TGGCTTAAGA AGCAAAAGTC ATATAATTTA GTAGTGGCCA AAGACGGAGG GGATAAAAAA 
GGGCCTACCC AAGCTGTTGT GGAAGAACAG ATCATAGATA AAGAGGAGTC TCTTCCAGAA 
GAAGAGAGGC AGGCCAAGGG TGATGCTGAA GAAACAGCTC GTAGAAAACA ACAGGAGATT 
AAGGCTGAAG TCAAGGAAGA GCAACCTTCA CCTGTTAAAG AAGAAAGTAA GGAGAGAGAA 
GAGGAGAAGG AGAAGGAAAT TCAGGAAGAA AAACCAGAAG GAGAAGCAGC AAAAAGGGAG 
ACAAAGGAAG TGCAGACCAA TGAGCTAAAA GCAGAGAAGA TCTCCCAAAA AGCCACCAAG 
AAGACCAAAA CTGTCCAGTG TAAAGTGACC CTCCTGGATG GCACCGAATA CAGCTGTGAC 
CTGGAGAAAC GTGCTAAGGG ACAAGTATTA TTTGACAAAG TATGTGAACA TCTCAATCTC 
TTGGAGAAGG ACTACTTTGG ACTTTTGTTT CAGGAAAATC CTGAGCAGAA AAACTGGCTA 
GATCCTGCAA AAGAAATAAA GAGACAGCTG CGAAATCTTC CCTGGCTGTT CACCTTTAAT 
GTGAAGTTTT ATCCTCCTGA TCCTTCTCAG TTGACTGAAG ATATCACCAG ATACTTCCTG 
TGCCTTCAGC TCCGGCAAGA CATTGCTTCT GGCCGCCTGC CCTGCTCCTT TGTGACTCAT 
GCCCTCCTGG GATCCTACAC ACTGCAGGCT GAACTCGGGG ACTATGATCC AGAAGAGCAT 
GGCCCTGATG TCTTCAGTGA CTTCCAGTTT GCCCCTACGC AGACTAAGGA GCTAGAAGAG 
AAGGTGGCAG AGCTACATAA AACCCACAGG GGCTTATCTC CAGCACAAGC TGATTCTCAG 
TTCTTAGAAA ATGCAAAGAG GCTCTCCATG TATGGTGTTG ACCTGCATCA TGCGAAGGTA 
CTGCTTGTGT CACCAGAGCA GCCACCAAAG GCCAAGTTCC TGACCTTGGG GTCCAAATTC 
CGCTACAGTG GCCGCACCCA AGCCCAGACC CGCCAGGCAA GCATCCTCAT TGATAGGCCA 
GCACCACATT TTGAGCGCAC TTCTAGTAAA CGGGTTTCCA GGAGTCTAGA TGGAGCTCTG 
ATTGGCGTTG TGGACCAAAG TCTTATGAAG GATTTTTCTG GCCCTGCTGG GGAGATTTCA 
GCATATGGTC CTGGAGTTCT GAGTCCTGTT GGGATACAAG ATGGAGATGG CAGAAGGGAA 
GTCAGAACCC CAAGTAAAGC CCCACATTTG CAGCTCATTG AAGGAAAGAA AAATTCCTTG 
AGAGTAGAAG GGGATAATAT TTATGTCAGA CATAGCAATT TAATGTTGGA GCCCAATGAA 
TGGGAAAAAC GACGTATCAC ACCTCTGTCC CTACAGACAC AAGGGAGTTC ACATGAGACT 
CTGAATGTAG TGGAGGAGAA GAAGCAGGCA GAGGTTGGGA AAGACGAAAA AGTAATCACA 
GAAGAAATGA ACGGTAAAGA GATATCACCT GGGAGTGGAC CTGGGGAGAT TCGTAAAGTG 
GAGCCACTGG CACAAAAAGA CTCCACCTCC CTGTCTTCTG AGAGCAGCAG CAGCAGCAGT 
GAGAGTGAAG AAGAAGAGGT GGGAGAGTAC CGCCCCTACC ACCGTGTGAC CGAGGGCACC 
ATAAGGGAGG AGCAGGAGGA GTATGAAGAA GAGACAGAGG AAGAGCCCGG CCGAGCAGCC 
AAGGTAGTAG AGAGGGAGGA AGCAGTGCCA GAAGCCAGCC CAGTCAAACA AGCAGGTGCC 
AATATAATCA CAATAGAAAC AGTGATCCAA GAAAATGTAG TTGCCCAAAA GGTATCTGGA 
GAGAAGAGTG TAAACGAAGG TGCTGTTAAT AAGCAAGACA CGAGAGAAGA AGCAGAGGAA 
GAGCAACATA AAGTTAACGG AGAGGTGTCT CATGTTGACA TTGATGTTTT GCCACAAATT 
ATTTGTTGTT CAGAGCCACC AGTGGTAAAA ACAGAGATGG TAACAATTTC TGATGCCTCA 
CAAAGGACAG AAATCTCCAC CAAGGAAGTC CCCATTGTCC AAACTGAGAC CAAAACCATC 
ACATATGAGT CTCCGCAGGT TGATGGCGGG GCTGGTGGTG ATTCGGGCAC ATTACTGACC 
GCACAAACCA TCACATCTGA GTCCGTGTCG ACAACAACCA CCACACACAT AACCAAGACT 
GTAAAAGGTG GAATATCTGA AACAAGAATT GAGAAACGTA TTGTGATCAC AGGAGATGCA 
GATATTGATC ATGACCAGGC ATTGGCCCAG GCGATCAAGG AAGCCAGAGA GCAGCACCCT 
GACATGTCCG TCACAAGAGT GGTGGTGCAC AAAGAAACAG AGCTGGCAGA AGAAGGGGAA 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012410365.1
CDS	1..2646
Translation	MTTEVGSVSEVKKESDQLGADATKEKPEEVAENQQNQSSDPEEEKGLQSPPAAESQSSPRRQKREKDPSESRGISRFIPPWLKKQKSYNLVVAKDGGDKKGPTQAVVEEQIIDKEESLPEEERQAKGDAEETARRKQQEIKAEVKEEQPSPVKEESKEREEEKEKEIQEEKPEGEAAKRETKEVQTNELKAEKISQKATKKTKTVQCKVTLLDGTEYSCDLEKRAKGQVLFDKVCEHLNLLEKDYFGLLFQENPEQKNWLDPAKEIKRQLRNLPWLFTFNVKFYPPDPSQLTEDITRYFLCLQLRQDIASGRLPCSFVTHALLGSYTLQAELGDYDPEEHGPDVFSDFQFAPTQTKELEEKVAELHKTHRGLSPAQADSQFLENAKRLSMYGVDLHHAKVLLVSPEQPPKAKFLTLGSKFRYSGRTQAQTRQASILIDRPAPHFERTSSKRVSRSLDGALIGVVDQSLMKDFSGPAGEISAYGPGVLSPVGIQDGDGRREVRTPSKAPHLQLIEGKKNSLRVEGDNIYVRHSNLMLEPNEWEKRRITPLSLQTQGSSHETLNVVEEKKQAEVGKDEKVITEEMNGKEISPGSGPGEIRKVEPLAQKDSTSLSSESSSSSSESEEEEVGEYRPYHRVTEGTIREEQEEYEEETEEEPGRAAKVVEREEAVPEASPVKQAGANIITIETVIQENVVAQKVSGEKSVNEGAVNKQDTREEAEEEQHKVNGEVSHVDIDVLPQIICCSEPPVVKTEMVTISDASQRTEISTKEVPIVQTETKTITYESPQVDGGAGGDSGTLLTAQTITSESVSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAREQHPDMSVTRVVVHKETELAEEGEE

Target ORF information:

RefSeq Version	XM_012554911.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris erythrocyte membrane protein band 4.1-like 2 (LOC101354974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012554911.1

ORF Insert Sequence:

ATGACCACTG AAGTAGGCTC CGTGTCTGAA GTGAAAAAGG AATCTGACCA GTTAGGAGCA 
GATGCAACCA AGGAGAAACC TGAAGAAGTA GCAGAAAATC AGCAGAATCA ATCATCCGAC 
CCAGAGGAGG AAAAAGGTCT CCAGTCACCT CCTGCAGCTG AAAGCCAAAG TAGTCCACGC 
CGCCAGAAGA GAGAGAAGGA TCCATCTGAG AGCAGGGGTA TTTCTCGATT CATACCCCCA 
TGGCTTAAGA AGCAAAAGTC ATATAATTTA GTAGTGGCCA AAGACGGAGG GGATAAAAAA 
GGGCCTACCC AAGCTGTTGT GGAAGAACAG ATCATAGATA AAGAGGAGTC TCTTCCAGAA 
GAAGAGAGGC AGGCCAAGGG TGATGCTGAA GAAACAGCTC GTAGAAAACA ACAGGAGATT 
AAGGCTGAAG TCAAGGAAGA GCAACCTTCA CCTGTTAAAG AAGAAAGTAA GGAGAGAGAA 
GAGGAGAAGG AGAAGGAAAT TCAGGAAGAA AAACCAGAAG GAGAAGCAGC AAAAAGGGAG 
ACAAAGGAAG TGCAGACCAA TGAGCTAAAA GCAGAGAAGA TCTCCCAAAA AGCCACCAAG 
AAGACCAAAA CTGTCCAGTG TAAAGTGACC CTCCTGGATG GCACCGAATA CAGCTGTGAC 
CTGGAGAAAC GTGCTAAGGG ACAAGTATTA TTTGACAAAG TATGTGAACA TCTCAATCTC 
TTGGAGAAGG ACTACTTTGG ACTTTTGTTT CAGGAAAATC CTGAGCAGAA AAACTGGCTA 
GATCCTGCAA AAGAAATAAA GAGACAGCTG CGAAATCTTC CCTGGCTGTT CACCTTTAAT 
GTGAAGTTTT ATCCTCCTGA TCCTTCTCAG TTGACTGAAG ATATCACCAG ATACTTCCTG 
TGCCTTCAGC TCCGGCAAGA CATTGCTTCT GGCCGCCTGC CCTGCTCCTT TGTGACTCAT 
GCCCTCCTGG GATCCTACAC ACTGCAGGCT GAACTCGGGG ACTATGATCC AGAAGAGCAT 
GGCCCTGATG TCTTCAGTGA CTTCCAGTTT GCCCCTACGC AGACTAAGGA GCTAGAAGAG 
AAGGTGGCAG AGCTACATAA AACCCACAGG GGCTTATCTC CAGCACAAGC TGATTCTCAG 
TTCTTAGAAA ATGCAAAGAG GCTCTCCATG TATGGTGTTG ACCTGCATCA TGCGAAGGTA 
CTGCTTGTGT CACCAGAGCA GCCACCAAAG GCCAAGTTCC TGACCTTGGG GTCCAAATTC 
CGCTACAGTG GCCGCACCCA AGCCCAGACC CGCCAGGCAA GCATCCTCAT TGATAGGCCA 
GCACCACATT TTGAGCGCAC TTCTAGTAAA CGGGTTTCCA GGAGTCTAGA TGGAGCTCTG 
ATTGGCGTTG TGGACCAAAG TCTTATGAAG GATTTTTCTG GCCCTGCTGG GGAGATTTCA 
GCATATGGTC CTGGAGTTCT GAGTCCTGTT GGGATACAAG ATGGAGATGG CAGAAGGGAA 
GTCAGAACCC CAAGTAAAGC CCCACATTTG CAGCTCATTG AAGGAAAGAA AAATTCCTTG 
AGAGTAGAAG GGGATAATAT TTATGTCAGA CATAGCAATT TAATGTTGGA GCCCAATGAA 
TGGGAAAAAC GACGTATCAC ACCTCTGTCC CTACAGACAC AAGGGAGTTC ACATGAGACT 
CTGAATGTAG TGGAGGAGAA GAAGCAGGCA GAGGTTGGGA AAGACGAAAA AGTAATCACA 
GAAGAAATGA ACGGTAAAGA GATATCACCT GGGAGTGGAC CTGGGGAGAT TCGTAAAGTG 
GAGCCACTGG CACAAAAAGA CTCCACCTCC CTGTCTTCTG AGAGCAGCAG CAGCAGCAGT 
GAGAGTGAAG AAGAAGAGGT GGGAGAGTAC CGCCCCTACC ACCGTGTGAC CGAGGGCACC 
ATAAGGGAGG AGCAGGAGGA GTATGAAGAA GAGACAGAGG AAGAGCCCGG CCGAGCAGCC 
AAGGTAGTAG AGAGGGAGGA AGCAGTGCCA GAAGCCAGCC CAGTCAAACA AGCAGGTGCC 
AATATAATCA CAATAGAAAC AGTGATCCAA GAAAATGTAG TTGCCCAAAA GGTATCTGGA 
GAGAAGAGTG TAAACGAAGG TGCTGTTAAT AAGCAAGACA CGAGAGAAGA AGCAGAGGAA 
GAGCAACATA AAGTTAACGG AGAGGTGTCT CATGTTGACA TTGATGTTTT GCCACAAATT 
ATTTGTTGTT CAGAGCCACC AGTGGTAAAA ACAGAGATGG TAACAATTTC TGATGCCTCA 
CAAAGGACAG AAATCTCCAC CAAGGAAGTC CCCATTGTCC AAACTGAGAC CAAAACCATC 
ACATATGAGT CTCCGCAGGT TGATGGCGGG GCTGGTGGTG ATTCGGGCAC ATTACTGACC 
GCACAAACCA TCACATCTGA GTCCGTGTCG ACAACAACCA CCACACACAT AACCAAGACT 
GTAAAAGGTG GAATATCTGA AACAAGAATT GAGAAACGTA TTGTGATCAC AGGAGATGCA 
GATATTGATC ATGACCAGGC ATTGGCCCAG GCGATCAAGG AAGCCAGAGA GCAGCACCCT 
GACATGTCCG TCACAAGAGT GGTGGTGCAC AAAGAAACAG AGCTGGCAGA AGAAGGGGAA 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101354974 ( XM_023727906.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023583674.1 Trichechus manatus latirostris erythrocyte membrane protein band 4.1 like 2 (LOC101354974), mRNA.

Price & Availability↑

CloneID	OTr214139
Clone ID Related Accession (Same CDS sequence)	XM_023727906.1
Accession Version	XM_023727906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	band 4.1-like protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443938.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGACCACTG AAGTAGGCTC CGTGTCTGAA GTGAAAAAGG AATCTGACCA GTTAGGAGCA 
GATGCAACCA AGGAGAAACC TGAAGAAGTA GCAGAAAATC AGCAGAATCA ATCATCCGAC 
CCAGAGGAGG AAAAAGGTCT CCAGTCACCT CCTGCAGCTG AAAGCCAAAG TAGTCCACGC 
CGCCAGAAGA GAGAGAAGGA TCCATCTGAG AGCAGGGGTA TTTCTCGATT CATACCCCCA 
TGGCTTAAGA AGCAAAAGTC ATATAATTTA GTAGTGGCCA AAGACGGAGG GGATAAAAAA 
GGGCCTACCC AAGCTGTTGT GGAAGAACAG ATCATAGATA AAGAGGAGTC TCTTCCAGAA 
GAAGAGAGGC AGGCCAAGGG TGATGCTGAA GAAACAGCTC GTAGAAAACA ACAGGAGATT 
AAGGCTGAAG TCAAGGAAGA GCAACCTTCA GTAAGCATTG CTGAGGCACA GCCTGTTAAA 
GAAGAAAGTA AGGAGAGAGA AGAGGAGAAG GAGAAGGAAA TTCAGGAAGA AAAACCAGAA 
GGAGAAGCAG CAAAAAGGGA GACAAAGGAA GTGCAGACCA ATGAGCTAAA AGCAGAGAAG 
ATCTCCCAAA AAGCCACCAA GAAGACCAAA ACTGTCCAGT GTAAAGTGAC CCTCCTGGAT 
GGCACCGAAT ACAGCTGTGA CCTGGAGAAA CGTGCTAAGG GACAAGTATT ATTTGACAAA 
GTATGTGAAC ATCTCAATCT CTTGGAGAAG GACTACTTTG GACTTTTGTT TCAGGAAAAT 
CCTGAGCAGA AAAACTGGCT AGATCCTGCA AAAGAAATAA AGAGACAGCT GCGAAATCTT 
CCCTGGCTGT TCACCTTTAA TGTGAAGTTT TATCCTCCTG ATCCTTCTCA GTTGACTGAA 
GATATCACCA GATACTTCCT GTGCCTTCAG CTCCGGCAAG ACATTGCTTC TGGCCGCCTG 
CCCTGCTCCT TTGTGACTCA TGCCCTCCTG GGATCCTACA CACTGCAGGC TGAACTCGGG 
GACTATGATC CAGAAGAGCA TGGCCCTGAT GTCTTCAGTG ACTTCCAGTT TGCCCCTACG 
CAGACTAAGG AGCTAGAAGA GAAGGTGGCA GAGCTACATA AAACCCACAG GGGCTTATCT 
CCAGCACAAG CTGATTCTCA GTTCTTAGAA AATGCAAAGA GGCTCTCCAT GTATGGTGTT 
GACCTGCATC ATGCGAAGGT ACTGCTTGTG TCACCAGAGC AGCCACCAAA GGCCAAGTTC 
CTGACCTTGG GGTCCAAATT CCGCTACAGT GGCCGCACCC AAGCCCAGAC CCGCCAGGCA 
AGCATCCTCA TTGATAGGCC AGCACCACAT TTTGAGCGCA CTTCTAGTAA ACGGGTTTCC 
AGGAGTCTAG ATGGAGCTCT GATTGGCGTT GTGGACCAAA GTCTTATGAA GGATTTTTCT 
GGCCCTGCTG GGGAGATTTC AGCATATGGT CCTGGAGTTC TGAGTCCTGT TGGGATACAA 
GATGGAGATG GCAGAAGGGA AGTCAGAACC CCAAGTAAAG CCCCACATTT GCAGCTCATT 
GAAGGAAAGA GTTCACATGA GACTCTGAAT GTAGTGGAGG AGAAGAAGCA GGCAGAGGTT 
GGGAAAGACG AAAAAGTAAT CACAGAAGAA ATGAACGGTA AAGAGATATC ACCTGGGAGT 
GGACCTGGGG AGATTCGTAA AGTGGAGCCA CTGGCACAAA AAGACTCCAC CTCCCTGTCT 
TCTGAGAGCA GCAGCAGCAG CAGTGAGAGT GAAGAAGAAG AGGTGGGAGA GTACCGCCCC 
TACCACCGTG TGACCGAGGG CACCATAAGG GAGGAGCAGG AGGAGTATGA AGAAGAGACA 
GAGGAAGAGC CCGGCCGAGC AGCCAAGGTA GTAGAGAGGG AGGAAGCAGT GCCAGAAGCC 
AGCCCAGTCA AACAAGCAGG TGCCAATATA ATCACAATAG AAACAGTGAT CCAAGAAAAT 
GTAGTTGCCC AAAAGGTATC TGGAGAGAAG AGTGTAAACG AAGGTGCTGT TAATAAGCAA 
GACACGAGAG AAGAAGCAGA GGAAGAGCAA CATAAAGTTA ACGGAGAGGT GTCTCATGTT 
GACATTGATG TTTTGCCACA AATTATTTGT TGTTCAGAGC CACCAGTGGT AAAAACAGAG 
ATGGTAACAA TTTCTGATGC CTCACAAAGG ACAGAAATCT CCACCAAGGA AGTCCCCATT 
GTCCAAACTG AGACCAAAAC CATCACATAT GAGTCTCCGC AGGTTGATGG CGGGGCTGGT 
GGTGATTCGG GCACATTACT GACCGCACAA ACCATCACAT CTGAGTCCGT GTCGACAACA 
ACCACCACAC ACATAACCAA GACTGTAAAA GGTGGAATAT CTGAAACAAG AATTGAGAAA 
CGTATTGTGA TCACAGGAGA TGCAGATATT GATCATGACC AGGCATTGGC CCAGGCGATC 
AAGGAAGCCA GAGAGCAGCA CCCTGACATG TCCGTCACAA GAGTGGTGGT GCACAAAGAA 
ACAGAGCTGG CAGAAGAAGG GGAAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023583674.1
CDS	118..2667
Translation	MTTEVGSVSEVKKESDQLGADATKEKPEEVAENQQNQSSDPEEEKGLQSPPAAESQSSPRRQKREKDPSESRGISRFIPPWLKKQKSYNLVVAKDGGDKKGPTQAVVEEQIIDKEESLPEEERQAKGDAEETARRKQQEIKAEVKEEQPSVSIAEAQPVKEESKEREEEKEKEIQEEKPEGEAAKRETKEVQTNELKAEKISQKATKKTKTVQCKVTLLDGTEYSCDLEKRAKGQVLFDKVCEHLNLLEKDYFGLLFQENPEQKNWLDPAKEIKRQLRNLPWLFTFNVKFYPPDPSQLTEDITRYFLCLQLRQDIASGRLPCSFVTHALLGSYTLQAELGDYDPEEHGPDVFSDFQFAPTQTKELEEKVAELHKTHRGLSPAQADSQFLENAKRLSMYGVDLHHAKVLLVSPEQPPKAKFLTLGSKFRYSGRTQAQTRQASILIDRPAPHFERTSSKRVSRSLDGALIGVVDQSLMKDFSGPAGEISAYGPGVLSPVGIQDGDGRREVRTPSKAPHLQLIEGKSSHETLNVVEEKKQAEVGKDEKVITEEMNGKEISPGSGPGEIRKVEPLAQKDSTSLSSESSSSSSESEEEEVGEYRPYHRVTEGTIREEQEEYEEETEEEPGRAAKVVEREEAVPEASPVKQAGANIITIETVIQENVVAQKVSGEKSVNEGAVNKQDTREEAEEEQHKVNGEVSHVDIDVLPQIICCSEPPVVKTEMVTISDASQRTEISTKEVPIVQTETKTITYESPQVDGGAGGDSGTLLTAQTITSESVSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAREQHPDMSVTRVVVHKETELAEEGEE

Target ORF information:

RefSeq Version	XM_023727906.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris erythrocyte membrane protein band 4.1 like 2 (LOC101354974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023727906.1

ORF Insert Sequence:

ATGACCACTG AAGTAGGCTC CGTGTCTGAA GTGAAAAAGG AATCTGACCA GTTAGGAGCA 
GATGCAACCA AGGAGAAACC TGAAGAAGTA GCAGAAAATC AGCAGAATCA ATCATCCGAC 
CCAGAGGAGG AAAAAGGTCT CCAGTCACCT CCTGCAGCTG AAAGCCAAAG TAGTCCACGC 
CGCCAGAAGA GAGAGAAGGA TCCATCTGAG AGCAGGGGTA TTTCTCGATT CATACCCCCA 
TGGCTTAAGA AGCAAAAGTC ATATAATTTA GTAGTGGCCA AAGACGGAGG GGATAAAAAA 
GGGCCTACCC AAGCTGTTGT GGAAGAACAG ATCATAGATA AAGAGGAGTC TCTTCCAGAA 
GAAGAGAGGC AGGCCAAGGG TGATGCTGAA GAAACAGCTC GTAGAAAACA ACAGGAGATT 
AAGGCTGAAG TCAAGGAAGA GCAACCTTCA GTAAGCATTG CTGAGGCACA GCCTGTTAAA 
GAAGAAAGTA AGGAGAGAGA AGAGGAGAAG GAGAAGGAAA TTCAGGAAGA AAAACCAGAA 
GGAGAAGCAG CAAAAAGGGA GACAAAGGAA GTGCAGACCA ATGAGCTAAA AGCAGAGAAG 
ATCTCCCAAA AAGCCACCAA GAAGACCAAA ACTGTCCAGT GTAAAGTGAC CCTCCTGGAT 
GGCACCGAAT ACAGCTGTGA CCTGGAGAAA CGTGCTAAGG GACAAGTATT ATTTGACAAA 
GTATGTGAAC ATCTCAATCT CTTGGAGAAG GACTACTTTG GACTTTTGTT TCAGGAAAAT 
CCTGAGCAGA AAAACTGGCT AGATCCTGCA AAAGAAATAA AGAGACAGCT GCGAAATCTT 
CCCTGGCTGT TCACCTTTAA TGTGAAGTTT TATCCTCCTG ATCCTTCTCA GTTGACTGAA 
GATATCACCA GATACTTCCT GTGCCTTCAG CTCCGGCAAG ACATTGCTTC TGGCCGCCTG 
CCCTGCTCCT TTGTGACTCA TGCCCTCCTG GGATCCTACA CACTGCAGGC TGAACTCGGG 
GACTATGATC CAGAAGAGCA TGGCCCTGAT GTCTTCAGTG ACTTCCAGTT TGCCCCTACG 
CAGACTAAGG AGCTAGAAGA GAAGGTGGCA GAGCTACATA AAACCCACAG GGGCTTATCT 
CCAGCACAAG CTGATTCTCA GTTCTTAGAA AATGCAAAGA GGCTCTCCAT GTATGGTGTT 
GACCTGCATC ATGCGAAGGT ACTGCTTGTG TCACCAGAGC AGCCACCAAA GGCCAAGTTC 
CTGACCTTGG GGTCCAAATT CCGCTACAGT GGCCGCACCC AAGCCCAGAC CCGCCAGGCA 
AGCATCCTCA TTGATAGGCC AGCACCACAT TTTGAGCGCA CTTCTAGTAA ACGGGTTTCC 
AGGAGTCTAG ATGGAGCTCT GATTGGCGTT GTGGACCAAA GTCTTATGAA GGATTTTTCT 
GGCCCTGCTG GGGAGATTTC AGCATATGGT CCTGGAGTTC TGAGTCCTGT TGGGATACAA 
GATGGAGATG GCAGAAGGGA AGTCAGAACC CCAAGTAAAG CCCCACATTT GCAGCTCATT 
GAAGGAAAGA GTTCACATGA GACTCTGAAT GTAGTGGAGG AGAAGAAGCA GGCAGAGGTT 
GGGAAAGACG AAAAAGTAAT CACAGAAGAA ATGAACGGTA AAGAGATATC ACCTGGGAGT 
GGACCTGGGG AGATTCGTAA AGTGGAGCCA CTGGCACAAA AAGACTCCAC CTCCCTGTCT 
TCTGAGAGCA GCAGCAGCAG CAGTGAGAGT GAAGAAGAAG AGGTGGGAGA GTACCGCCCC 
TACCACCGTG TGACCGAGGG CACCATAAGG GAGGAGCAGG AGGAGTATGA AGAAGAGACA 
GAGGAAGAGC CCGGCCGAGC AGCCAAGGTA GTAGAGAGGG AGGAAGCAGT GCCAGAAGCC 
AGCCCAGTCA AACAAGCAGG TGCCAATATA ATCACAATAG AAACAGTGAT CCAAGAAAAT 
GTAGTTGCCC AAAAGGTATC TGGAGAGAAG AGTGTAAACG AAGGTGCTGT TAATAAGCAA 
GACACGAGAG AAGAAGCAGA GGAAGAGCAA CATAAAGTTA ACGGAGAGGT GTCTCATGTT 
GACATTGATG TTTTGCCACA AATTATTTGT TGTTCAGAGC CACCAGTGGT AAAAACAGAG 
ATGGTAACAA TTTCTGATGC CTCACAAAGG ACAGAAATCT CCACCAAGGA AGTCCCCATT 
GTCCAAACTG AGACCAAAAC CATCACATAT GAGTCTCCGC AGGTTGATGG CGGGGCTGGT 
GGTGATTCGG GCACATTACT GACCGCACAA ACCATCACAT CTGAGTCCGT GTCGACAACA 
ACCACCACAC ACATAACCAA GACTGTAAAA GGTGGAATAT CTGAAACAAG AATTGAGAAA 
CGTATTGTGA TCACAGGAGA TGCAGATATT GATCATGACC AGGCATTGGC CCAGGCGATC 
AAGGAAGCCA GAGAGCAGCA CCCTGACATG TCCGTCACAA GAGTGGTGGT GCACAAAGAA 
ACAGAGCTGG CAGAAGAAGG GGAAGAGTAG