LOC101354647 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101354647
Entrez Gene ID	101354647
Full Name	dihydrolipoamide S-succinyltransferase
Synonyms	DLST
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004371045.1	XP_004371102.1	dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial
XM_004371045.2	XP_004371102.1	dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC101354647 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101354647 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr167212	XM_004371045.2 Latest version!	Trichechus manatus latirostris dihydrolipoamide S-succinyltransferase (LOC101354647), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OTr167212	XM_004371045.1	Trichechus manatus latirostris dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (LOC101354647), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101354647 ( XM_004371045.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004371102.1 Trichechus manatus latirostris dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (LOC101354647), mRNA.

Price & Availability↑

CloneID	OTr167212
Clone ID Related Accession (Same CDS sequence)	XM_004371045.1 , XM_004371045.2
Accession Version	XM_004371045.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443946.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTATCCC GGTCTCGCTG CGTGTCCCGG GCGTTCAGCC GTTCGCTCTC CGCCTTCCAG 
AAGGGGAACT GCCCTCTAGG GAGACGTTCC CTGCCTGGGG TCTCGTTATG TCAGGGACCA 
GGTTACCCTG ACAGCAGGAA GACTGTCGTT AACAGCAGCA GTGTCCTCAG TGTTCGCTTC 
TTCAGAACTA CGGCCGCATG CAAGGATGAT GTGATTACAG TCAAAACCCC GGCGTTTGCA 
GAGTCTGTCA CAGAGGGAGA CGTCAGGTGG GAGAAAGCTG TTGGAGACTC AGTTGCAGAA 
GATGAAGTGG TTTGTGAGAT TGAAACTGAC AAGACATCTG TGCAGGTTCC ATCACCAGCA 
AATGGCGTGA TTGAAGCTCT TTTGGTACCT GATGGGGGAA AAGTGGAAGG AGGCACTCCT 
CTTTTCACAC TCAGGAAAAC TGATGCTGCT CCTGCTAAGG CTAAGCCAGC TGAAGCTCCT 
GCTGCTGCAG CCCCCAAAGC AGAGCCTATG GCAGCAGCAG TTCCTCCTCC CTCTGCAGCA 
GCCATACCCA CTCAGATGCC ACCAGTGCCC TCGCCTGCAC AACCTCCTGC TAGCAAACCG 
GTATCTGCAA TAAAACCTAC TGCTGCTCCC CCAGTAGCTG ACCTAGGAGC TGGCAAAGGT 
CTCCGTTCAG AACACCGGGA GAAAATGAAC AGGATGCGGC AGCGCATTGC TCAGCGTCTG 
AAAGAGGCCC AGAATACATG TGCAATGCTG ACTACTTTCA ATGAGGTTGA CATGAGTAAC 
ATCCAGGAGA TGAGGGCTCG GCACAGAGAT GCTTTTCTGA AGAAACATAA CCTCAAACTA 
GGTTTCATGT CAGCATTTGT GAAGGCCTCG GCCTATGCCT TGCAGGAACA GCCTGTTGTA 
AATGCAGTGA TTGACGATAC GGCCAAAGAG GTGGTGTATA GGGATTATAT TGACATCAGT 
GTTGCAGTGG CCACCCCACG GGGTCTGGTG GTTCCTGTCA TCAGGAATGT GGAAGCTATG 
AATTTTGCAG ATATTGAGCG AACCATCAGT GAACTGGGAG AGAAGGCCCG AAAGAATGAA 
CTTGCCATTG AAGATATGGA TGGCGGCACT TTCACCATTA GCAATGGAGG CGTTTTTGGC 
TCGCTCTTTG GAACACCCAT TATCAACCCC CCTCAGTCTG CCATCCTGGG CATGCATGGC 
ATCTTTGATA GGCCAGTGGC CGTAGGAGGC AAGTTGGAGG TGCGGCCCAT GATGTATGTG 
GCACTGACCT ACGACCACCG GCTGATCGAT GGCAGAGAGG CAGTGACTTT CCTCCGCAAA 
ATCAAGGCAG CGGTAGAGGA TCCCAGAGTC CTCCTACTGG ATCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004371102.1
CDS	1..1368
Translation	MLSRSRCVSRAFSRSLSAFQKGNCPLGRRSLPGVSLCQGPGYPDSRKTVVNSSSVLSVRFFRTTAACKDDVITVKTPAFAESVTEGDVRWEKAVGDSVAEDEVVCEIETDKTSVQVPSPANGVIEALLVPDGGKVEGGTPLFTLRKTDAAPAKAKPAEAPAAAAPKAEPMAAAVPPPSAAAIPTQMPPVPSPAQPPASKPVSAIKPTAAPPVADLGAGKGLRSEHREKMNRMRQRIAQRLKEAQNTCAMLTTFNEVDMSNIQEMRARHRDAFLKKHNLKLGFMSAFVKASAYALQEQPVVNAVIDDTAKEVVYRDYIDISVAVATPRGLVVPVIRNVEAMNFADIERTISELGEKARKNELAIEDMDGGTFTISNGGVFGSLFGTPIINPPQSAILGMHGIFDRPVAVGGKLEVRPMMYVALTYDHRLIDGREAVTFLRKIKAAVEDPRVLLLDL

Target ORF information:

RefSeq Version	XM_004371045.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (LOC101354647), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004371045.1

ORF Insert Sequence:

ATGCTATCCC GGTCTCGCTG CGTGTCCCGG GCGTTCAGCC GTTCGCTCTC CGCCTTCCAG 
AAGGGGAACT GCCCTCTAGG GAGACGTTCC CTGCCTGGGG TCTCGTTATG TCAGGGACCA 
GGTTACCCTG ACAGCAGGAA GACTGTCGTT AACAGCAGCA GTGTCCTCAG TGTTCGCTTC 
TTCAGAACTA CGGCCGCATG CAAGGATGAT GTGATTACAG TCAAAACCCC GGCGTTTGCA 
GAGTCTGTCA CAGAGGGAGA CGTCAGGTGG GAGAAAGCTG TTGGAGACTC AGTTGCAGAA 
GATGAAGTGG TTTGTGAGAT TGAAACTGAC AAGACATCTG TGCAGGTTCC ATCACCAGCA 
AATGGCGTGA TTGAAGCTCT TTTGGTACCT GATGGGGGAA AAGTGGAAGG AGGCACTCCT 
CTTTTCACAC TCAGGAAAAC TGATGCTGCT CCTGCTAAGG CTAAGCCAGC TGAAGCTCCT 
GCTGCTGCAG CCCCCAAAGC AGAGCCTATG GCAGCAGCAG TTCCTCCTCC CTCTGCAGCA 
GCCATACCCA CTCAGATGCC ACCAGTGCCC TCGCCTGCAC AACCTCCTGC TAGCAAACCG 
GTATCTGCAA TAAAACCTAC TGCTGCTCCC CCAGTAGCTG ACCTAGGAGC TGGCAAAGGT 
CTCCGTTCAG AACACCGGGA GAAAATGAAC AGGATGCGGC AGCGCATTGC TCAGCGTCTG 
AAAGAGGCCC AGAATACATG TGCAATGCTG ACTACTTTCA ATGAGGTTGA CATGAGTAAC 
ATCCAGGAGA TGAGGGCTCG GCACAGAGAT GCTTTTCTGA AGAAACATAA CCTCAAACTA 
GGTTTCATGT CAGCATTTGT GAAGGCCTCG GCCTATGCCT TGCAGGAACA GCCTGTTGTA 
AATGCAGTGA TTGACGATAC GGCCAAAGAG GTGGTGTATA GGGATTATAT TGACATCAGT 
GTTGCAGTGG CCACCCCACG GGGTCTGGTG GTTCCTGTCA TCAGGAATGT GGAAGCTATG 
AATTTTGCAG ATATTGAGCG AACCATCAGT GAACTGGGAG AGAAGGCCCG AAAGAATGAA 
CTTGCCATTG AAGATATGGA TGGCGGCACT TTCACCATTA GCAATGGAGG CGTTTTTGGC 
TCGCTCTTTG GAACACCCAT TATCAACCCC CCTCAGTCTG CCATCCTGGG CATGCATGGC 
ATCTTTGATA GGCCAGTGGC CGTAGGAGGC AAGTTGGAGG TGCGGCCCAT GATGTATGTG 
GCACTGACCT ACGACCACCG GCTGATCGAT GGCAGAGAGG CAGTGACTTT CCTCCGCAAA 
ATCAAGGCAG CGGTAGAGGA TCCCAGAGTC CTCCTACTGG ATCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101354647 ( XM_004371045.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004371102.1 Trichechus manatus latirostris dihydrolipoamide S-succinyltransferase (LOC101354647), mRNA.

Price & Availability↑

CloneID	OTr167212
Clone ID Related Accession (Same CDS sequence)	XM_004371045.1 , XM_004371045.2
Accession Version	XM_004371045.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443946.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_004371045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTATCCC GGTCTCGCTG CGTGTCCCGG GCGTTCAGCC GTTCGCTCTC CGCCTTCCAG 
AAGGGGAACT GCCCTCTAGG GAGACGTTCC CTGCCTGGGG TCTCGTTATG TCAGGGACCA 
GGTTACCCTG ACAGCAGGAA GACTGTCGTT AACAGCAGCA GTGTCCTCAG TGTTCGCTTC 
TTCAGAACTA CGGCCGCATG CAAGGATGAT GTGATTACAG TCAAAACCCC GGCGTTTGCA 
GAGTCTGTCA CAGAGGGAGA CGTCAGGTGG GAGAAAGCTG TTGGAGACTC AGTTGCAGAA 
GATGAAGTGG TTTGTGAGAT TGAAACTGAC AAGACATCTG TGCAGGTTCC ATCACCAGCA 
AATGGCGTGA TTGAAGCTCT TTTGGTACCT GATGGGGGAA AAGTGGAAGG AGGCACTCCT 
CTTTTCACAC TCAGGAAAAC TGATGCTGCT CCTGCTAAGG CTAAGCCAGC TGAAGCTCCT 
GCTGCTGCAG CCCCCAAAGC AGAGCCTATG GCAGCAGCAG TTCCTCCTCC CTCTGCAGCA 
GCCATACCCA CTCAGATGCC ACCAGTGCCC TCGCCTGCAC AACCTCCTGC TAGCAAACCG 
GTATCTGCAA TAAAACCTAC TGCTGCTCCC CCAGTAGCTG ACCTAGGAGC TGGCAAAGGT 
CTCCGTTCAG AACACCGGGA GAAAATGAAC AGGATGCGGC AGCGCATTGC TCAGCGTCTG 
AAAGAGGCCC AGAATACATG TGCAATGCTG ACTACTTTCA ATGAGGTTGA CATGAGTAAC 
ATCCAGGAGA TGAGGGCTCG GCACAGAGAT GCTTTTCTGA AGAAACATAA CCTCAAACTA 
GGTTTCATGT CAGCATTTGT GAAGGCCTCG GCCTATGCCT TGCAGGAACA GCCTGTTGTA 
AATGCAGTGA TTGACGATAC GGCCAAAGAG GTGGTGTATA GGGATTATAT TGACATCAGT 
GTTGCAGTGG CCACCCCACG GGGTCTGGTG GTTCCTGTCA TCAGGAATGT GGAAGCTATG 
AATTTTGCAG ATATTGAGCG AACCATCAGT GAACTGGGAG AGAAGGCCCG AAAGAATGAA 
CTTGCCATTG AAGATATGGA TGGCGGCACT TTCACCATTA GCAATGGAGG CGTTTTTGGC 
TCGCTCTTTG GAACACCCAT TATCAACCCC CCTCAGTCTG CCATCCTGGG CATGCATGGC 
ATCTTTGATA GGCCAGTGGC CGTAGGAGGC AAGTTGGAGG TGCGGCCCAT GATGTATGTG 
GCACTGACCT ACGACCACCG GCTGATCGAT GGCAGAGAGG CAGTGACTTT CCTCCGCAAA 
ATCAAGGCAG CGGTAGAGGA TCCCAGAGTC CTCCTACTGG ATCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004371102.1
CDS	33..1400
Translation	MLSRSRCVSRAFSRSLSAFQKGNCPLGRRSLPGVSLCQGPGYPDSRKTVVNSSSVLSVRFFRTTAACKDDVITVKTPAFAESVTEGDVRWEKAVGDSVAEDEVVCEIETDKTSVQVPSPANGVIEALLVPDGGKVEGGTPLFTLRKTDAAPAKAKPAEAPAAAAPKAEPMAAAVPPPSAAAIPTQMPPVPSPAQPPASKPVSAIKPTAAPPVADLGAGKGLRSEHREKMNRMRQRIAQRLKEAQNTCAMLTTFNEVDMSNIQEMRARHRDAFLKKHNLKLGFMSAFVKASAYALQEQPVVNAVIDDTAKEVVYRDYIDISVAVATPRGLVVPVIRNVEAMNFADIERTISELGEKARKNELAIEDMDGGTFTISNGGVFGSLFGTPIINPPQSAILGMHGIFDRPVAVGGKLEVRPMMYVALTYDHRLIDGREAVTFLRKIKAAVEDPRVLLLDL

Target ORF information:

RefSeq Version	XM_004371045.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris dihydrolipoamide S-succinyltransferase (LOC101354647), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004371045.2

ORF Insert Sequence:

ATGCTATCCC GGTCTCGCTG CGTGTCCCGG GCGTTCAGCC GTTCGCTCTC CGCCTTCCAG 
AAGGGGAACT GCCCTCTAGG GAGACGTTCC CTGCCTGGGG TCTCGTTATG TCAGGGACCA 
GGTTACCCTG ACAGCAGGAA GACTGTCGTT AACAGCAGCA GTGTCCTCAG TGTTCGCTTC 
TTCAGAACTA CGGCCGCATG CAAGGATGAT GTGATTACAG TCAAAACCCC GGCGTTTGCA 
GAGTCTGTCA CAGAGGGAGA CGTCAGGTGG GAGAAAGCTG TTGGAGACTC AGTTGCAGAA 
GATGAAGTGG TTTGTGAGAT TGAAACTGAC AAGACATCTG TGCAGGTTCC ATCACCAGCA 
AATGGCGTGA TTGAAGCTCT TTTGGTACCT GATGGGGGAA AAGTGGAAGG AGGCACTCCT 
CTTTTCACAC TCAGGAAAAC TGATGCTGCT CCTGCTAAGG CTAAGCCAGC TGAAGCTCCT 
GCTGCTGCAG CCCCCAAAGC AGAGCCTATG GCAGCAGCAG TTCCTCCTCC CTCTGCAGCA 
GCCATACCCA CTCAGATGCC ACCAGTGCCC TCGCCTGCAC AACCTCCTGC TAGCAAACCG 
GTATCTGCAA TAAAACCTAC TGCTGCTCCC CCAGTAGCTG ACCTAGGAGC TGGCAAAGGT 
CTCCGTTCAG AACACCGGGA GAAAATGAAC AGGATGCGGC AGCGCATTGC TCAGCGTCTG 
AAAGAGGCCC AGAATACATG TGCAATGCTG ACTACTTTCA ATGAGGTTGA CATGAGTAAC 
ATCCAGGAGA TGAGGGCTCG GCACAGAGAT GCTTTTCTGA AGAAACATAA CCTCAAACTA 
GGTTTCATGT CAGCATTTGT GAAGGCCTCG GCCTATGCCT TGCAGGAACA GCCTGTTGTA 
AATGCAGTGA TTGACGATAC GGCCAAAGAG GTGGTGTATA GGGATTATAT TGACATCAGT 
GTTGCAGTGG CCACCCCACG GGGTCTGGTG GTTCCTGTCA TCAGGAATGT GGAAGCTATG 
AATTTTGCAG ATATTGAGCG AACCATCAGT GAACTGGGAG AGAAGGCCCG AAAGAATGAA 
CTTGCCATTG AAGATATGGA TGGCGGCACT TTCACCATTA GCAATGGAGG CGTTTTTGGC 
TCGCTCTTTG GAACACCCAT TATCAACCCC CCTCAGTCTG CCATCCTGGG CATGCATGGC 
ATCTTTGATA GGCCAGTGGC CGTAGGAGGC AAGTTGGAGG TGCGGCCCAT GATGTATGTG 
GCACTGACCT ACGACCACCG GCTGATCGAT GGCAGAGAGG CAGTGACTTT CCTCCGCAAA 
ATCAAGGCAG CGGTAGAGGA TCCCAGAGTC CTCCTACTGG ATCTTTAG