LOC101354346 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101354346
Entrez Gene ID	101354346
Full Name	chitinase, acidic
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012556678.1	XP_012412132.1	LOW QUALITY PROTEIN: acidic mammalian chitinase

Pathways from BioSystems
Gene Ontology

The following LOC101354346 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101354346 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTr177655	XM_012556678.1 Latest version!	Trichechus manatus latirostris chitinase, acidic (LOC101354346), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101354346 ( XM_012556678.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012412132.1 Trichechus manatus latirostris chitinase, acidic (LOC101354346), mRNA.

Price & Availability↑

CloneID	OTr177655
Clone ID Related Accession (Same CDS sequence)	XM_012556678.1
Accession Version	XM_012556678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	LOW QUALITY PROTEIN: acidic mammalian chitinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444009.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels internal stop codons :: corrected 3 genomic stop codons ##RefSeq-Attributes-END##

ATGGCAAAGC TTATTCTACT TACGGGTCTA ACCCTTCTGC TGAATTCTCA GCTGGGTTCT 
ACCTACTTTC TGACATGCTA CTTCACCAAC TGGGCCCAGT ACCGGCCAGG CACTGGGCAC 
ATCAATCCTG ATGACATCAA CCCGNNTCTC TGTACTCACC TGATCTATGC CTTTGCTGGG 
ATGCACAACA ATGAGATCAC CACCACTCAG TGGAAAGATG TGACTCTCTA TCAAGCTTTC 
AGTGGCCTGA AAAACAAGAA TAGCCAGCTG AAAACTCTCC TGGCCATTAG AGGCTGGAAC 
TTTGGAACTG CCCTATTCAC TGCTATGGTT TCCACTCCTG AGAACTGAAA GACTTTCATT 
GCCTCAGTCA TCAAATTCCT GTGTCAGTAT GAGTTTGACA GGCTGGACTT TGACTAGGAG 
TATCCTGGCT CTCGTGGGAG CCCTCCTCAG GATAAGCACC TCTTCACCCT CCTGGTGCAG 
GAAATGCATC AAGCTTTTAA GCAGGAAACC AAGCAGGTCA ACAAGTCCAG GCTGATGATC 
ACCACTGCGG TAGCTGCTGG CATCTCTAAC ATCNCATCTG GCTATGAGAT CCCCCAGCTG 
TCCCAGTACC TGAACTACAT CCATGACATG ACCTATGACT TCCATGGTTC CTGGGAGAGC 
AACACTGGAG AGAACAGCCC CTTCTACAAG TATCCCGGTG ACACTGGCAG CAACGCCTCC 
CTCATTGTGG AATATGCCAT GAACTACTGG AAGAATAATG GACCCCCAGC CGAGAAGCTC 
ATCATTGGAT TCTCAACCTA TGGACACACC TGTATCCTGA GCAACCCCTC CAATACCAGC 
ATTGGTGCAC CCACCTCTGA TGTTGGTCCT GCTGGGCCCT ACACCAGGCA GGCTGGATTC 
TGGGCATACT ATGAGATCTA TACTTTTCTG AAAAATGGAG CCACTCAGGA ACGGGATGCC 
CCTCAGGATG TGCCTTATGC CTATCAGGGC AATGAGTGAG TTGGTTATGA CAACGACAAG 
AGCTTCAATA TCAAGGCTCA ATGGCTTAAG CAGAGCAACT TTGGAGGCAC CAAGATCTGG 
GCCATTGGCC TGGATGACTT CACTGGTAAT TTCTGCAGCC AGAGCAAGCT CCCCCTGATC 
TCCATCCTGA AGAATGCTCT TGGCTTGCAG AGTGCAAGTT GCACCCCACC AGCTCAGCCC 
ATTGCCCCAA TAACACTTTC TCCCAGCAGT GGAAGCAGAA GTGGGAGCGG AAGTGGGAGT 
GGAAGCAGCA GCTCTGGAGG CAGCTATGGG GGCAGTGGAT TCTGGGCTGG CAAAGCCAAT 
GGACTGTTCC CTGTGGCTAG TAAAAGAAAT GCCTTCTGGC ACTGCCTGAA TGGAATCACA 
TACCAGCAAC ACTGCCAGGC TGGGTTTGTC TTTGACATCA GCTGTGATTG CTGCAACTGG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012412132.1
CDS	1..1446
Translation	MAKLILLTGLTLLLNSQLGSTYFLTCYFTNWAQYRPGTGHINPDDINPnullLCTHLIYAFAGMHNNEITTTQWKDVTLYQAFSGLKNKNSQLKTLLAIRGWNFGTALFTAMVSTPENKTFIASVIKFLCQYEFDRLDFDEYPGSRGSPPQDKHLFTLLVQEMHQAFKQETKQVNKSRLMITTAVAAGISNInullSGYEIPQLSQYLNYIHDMTYDFHGSWESNTGENSPFYKYPGDTGSNASLIVEYAMNYWKNNGPPAEKLIIGFSTYGHTCILSNPSNTSIGAPTSDVGPAGPYTRQAGFWAYYEIYTFLKNGATQERDAPQDVPYAYQGNE*VGYDNDKSFNIKAQWLKQSNFGGTKIWAIGLDDFTGNFCSQSKLPLISILKNALGLQSASCTPPAQPIAPITLSPSSGSRSGSGSGSGSSSSGGSYGGSGFWAGKANGLFPVASKRNAFWHCLNGITYQQHCQAGFVFDISCDCCNWA

Target ORF information:

RefSeq Version	XM_012556678.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris chitinase, acidic (LOC101354346), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012556678.1

ORF Insert Sequence:

ATGGCAAAGC TTATTCTACT TACGGGTCTA ACCCTTCTGC TGAATTCTCA GCTGGGTTCT 
ACCTACTTTC TGACATGCTA CTTCACCAAC TGGGCCCAGT ACCGGCCAGG CACTGGGCAC 
ATCAATCCTG ATGACATCAA CCCGNNTCTC TGTACTCACC TGATCTATGC CTTTGCTGGG 
ATGCACAACA ATGAGATCAC CACCACTCAG TGGAAAGATG TGACTCTCTA TCAAGCTTTC 
AGTGGCCTGA AAAACAAGAA TAGCCAGCTG AAAACTCTCC TGGCCATTAG AGGCTGGAAC 
TTTGGAACTG CCCTATTCAC TGCTATGGTT TCCACTCCTG AGAACTGAAA GACTTTCATT 
GCCTCAGTCA TCAAATTCCT GTGTCAGTAT GAGTTTGACA GGCTGGACTT TGACTAGGAG 
TATCCTGGCT CTCGTGGGAG CCCTCCTCAG GATAAGCACC TCTTCACCCT CCTGGTGCAG 
GAAATGCATC AAGCTTTTAA GCAGGAAACC AAGCAGGTCA ACAAGTCCAG GCTGATGATC 
ACCACTGCGG TAGCTGCTGG CATCTCTAAC ATCNCATCTG GCTATGAGAT CCCCCAGCTG 
TCCCAGTACC TGAACTACAT CCATGACATG ACCTATGACT TCCATGGTTC CTGGGAGAGC 
AACACTGGAG AGAACAGCCC CTTCTACAAG TATCCCGGTG ACACTGGCAG CAACGCCTCC 
CTCATTGTGG AATATGCCAT GAACTACTGG AAGAATAATG GACCCCCAGC CGAGAAGCTC 
ATCATTGGAT TCTCAACCTA TGGACACACC TGTATCCTGA GCAACCCCTC CAATACCAGC 
ATTGGTGCAC CCACCTCTGA TGTTGGTCCT GCTGGGCCCT ACACCAGGCA GGCTGGATTC 
TGGGCATACT ATGAGATCTA TACTTTTCTG AAAAATGGAG CCACTCAGGA ACGGGATGCC 
CCTCAGGATG TGCCTTATGC CTATCAGGGC AATGAGTGAG TTGGTTATGA CAACGACAAG 
AGCTTCAATA TCAAGGCTCA ATGGCTTAAG CAGAGCAACT TTGGAGGCAC CAAGATCTGG 
GCCATTGGCC TGGATGACTT CACTGGTAAT TTCTGCAGCC AGAGCAAGCT CCCCCTGATC 
TCCATCCTGA AGAATGCTCT TGGCTTGCAG AGTGCAAGTT GCACCCCACC AGCTCAGCCC 
ATTGCCCCAA TAACACTTTC TCCCAGCAGT GGAAGCAGAA GTGGGAGCGG AAGTGGGAGT 
GGAAGCAGCA GCTCTGGAGG CAGCTATGGG GGCAGTGGAT TCTGGGCTGG CAAAGCCAAT 
GGACTGTTCC CTGTGGCTAG TAAAAGAAAT GCCTTCTGGC ACTGCCTGAA TGGAATCACA 
TACCAGCAAC ACTGCCAGGC TGGGTTTGTC TTTGACATCA GCTGTGATTG CTGCAACTGG 
GCATAA