LOC101352929 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101352929
Entrez Gene ID	101352929
Full Name	EWS RNA binding protein 1
Synonyms	EWSR1
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004387610.1	XP_004387667.1	RNA-binding protein EWS isoform X1
XM_004387612.1	XP_004387669.1	RNA-binding protein EWS isoform X4
XM_004387613.2	XP_004387670.1	RNA-binding protein EWS isoform X4
XM_004387613.1	XP_004387670.1	RNA-binding protein EWS isoform X5
XM_004387611.1	XP_004387668.1	RNA-binding protein EWS isoform X1
XM_012558847.1	XP_012414301.1	RNA-binding protein EWS isoform X2
XM_023741771.1	XP_023597539.1	RNA-binding protein EWS isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC101352929 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101352929 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr226178	XM_023741771.1 Latest version!	Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OTr185782	XM_004387610.1 Latest version!	Trichechus manatus latirostris EWS RNA-binding protein 1 (LOC101352929), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OTr185783	XM_004387611.1 Latest version!	Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OTr185784	XM_012558847.1 Latest version!	Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OTr185785	XM_004387612.1 Latest version!	Trichechus manatus latirostris EWS RNA-binding protein 1 (LOC101352929), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OTr185786	XM_004387613.2 Latest version!	Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
View Old Accession Versions >>
OTr185786	XM_004387613.1	Trichechus manatus latirostris EWS RNA-binding protein 1 (LOC101352929), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101352929 ( XM_023741771.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023597539.1 Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTr226178
Clone ID Related Accession (Same CDS sequence)	XM_023741771.1
Accession Version	XM_023741771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	RNA-binding protein EWS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444126.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGACCGCAG 
GATGGTAGCA AGCCTGCTGA GACTAGTCAA CCTCAGTCTA GCACAGGAGG TTACAACCAG 
CCCAGCATGG GGTATGGACA GAGTAACTAC AGTTATCCCC AAGTACCTGG GAGCTACCCC 
ATGCAGCCAG TCACCGCACC TCCATCCTAT CCTCCTACCA GCTATTCCTC TTCACAGCCA 
ACTAGCTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAGCC AAGCAGCTAT 
GGACAGCAGA GTAGCTATGG GCAACAGAGC AGCTATGGGC AGCAGCCGCC CACTAGTTAT 
CCCCCCCAAA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGTCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GACGAAGGAC CAGATCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCGATTT ATGTGCAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GACAGACTTC 
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAGCCCAT GATCCACATC 
TACCTGGACA AGGAAACTGG GAAGCCTAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA 
CCAACTGCCA AGGCTGCCGT GGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAGCTT 
AAAGTCTCTC TTGCTCGGAA GAAGCCTCCT ATGAACAGCA TGCGGGGCGG AATGCCACCC 
CGTGAGGGCA GAGGGATGCC ACCGCCACTC CGTGGAGGTC CGGGGGGCCC AGGAGGTCCT 
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGACA GAGGAGGCTT CCCCCCAAGA 
GGACCCCGGG GTTCCCGAGG GAACCCATCT GGAGGAGGAA ATGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGTTGTGGA AACCAGAACT TCGCCTGGAG AACAGAATGC 
AACCAGTGTG GTGACCGTGG CAGAGGTGGC CCTGGTGGCA TGCGGGGTGG GAGAGGTGGC 
CTCATGGACC GCGGTGGTCC TGGTGGAATG TTCAGAGGTG GCCGTGGTGG AGACAGAGGT 
GGCTTCCGTG GCGGCCGGGG CATGGACCGA GGTGGCTTTG GTGGAGGAAG ACGAGGCGGT 
CCCGGAGGGC CCCCTGGACC TTTGATGGAA CAGATGGGAG GAAGAAGAGG TGGACGCGGA 
GGACCTGGGA AGATGGATAA AGGCGAGCAC CGCCAGGAGC GCAGAGACCG GCCCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023597539.1
CDS	330..2249
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGSKPAETSQPQSSTGGYNQPSMGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSSQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLTDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	XM_023741771.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023741771.1

ORF Insert Sequence:

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGACCGCAG 
GATGGTAGCA AGCCTGCTGA GACTAGTCAA CCTCAGTCTA GCACAGGAGG TTACAACCAG 
CCCAGCATGG GGTATGGACA GAGTAACTAC AGTTATCCCC AAGTACCTGG GAGCTACCCC 
ATGCAGCCAG TCACCGCACC TCCATCCTAT CCTCCTACCA GCTATTCCTC TTCACAGCCA 
ACTAGCTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAGCC AAGCAGCTAT 
GGACAGCAGA GTAGCTATGG GCAACAGAGC AGCTATGGGC AGCAGCCGCC CACTAGTTAT 
CCCCCCCAAA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGTCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GACGAAGGAC CAGATCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCGATTT ATGTGCAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GACAGACTTC 
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAGCCCAT GATCCACATC 
TACCTGGACA AGGAAACTGG GAAGCCTAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA 
CCAACTGCCA AGGCTGCCGT GGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAGCTT 
AAAGTCTCTC TTGCTCGGAA GAAGCCTCCT ATGAACAGCA TGCGGGGCGG AATGCCACCC 
CGTGAGGGCA GAGGGATGCC ACCGCCACTC CGTGGAGGTC CGGGGGGCCC AGGAGGTCCT 
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGACA GAGGAGGCTT CCCCCCAAGA 
GGACCCCGGG GTTCCCGAGG GAACCCATCT GGAGGAGGAA ATGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGTTGTGGA AACCAGAACT TCGCCTGGAG AACAGAATGC 
AACCAGTGTG GTGACCGTGG CAGAGGTGGC CCTGGTGGCA TGCGGGGTGG GAGAGGTGGC 
CTCATGGACC GCGGTGGTCC TGGTGGAATG TTCAGAGGTG GCCGTGGTGG AGACAGAGGT 
GGCTTCCGTG GCGGCCGGGG CATGGACCGA GGTGGCTTTG GTGGAGGAAG ACGAGGCGGT 
CCCGGAGGGC CCCCTGGACC TTTGATGGAA CAGATGGGAG GAAGAAGAGG TGGACGCGGA 
GGACCTGGGA AGATGGATAA AGGCGAGCAC CGCCAGGAGC GCAGAGACCG GCCCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101352929 ( XM_004387610.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004387667.1 Trichechus manatus latirostris EWS RNA-binding protein 1 (LOC101352929), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTr185782
Clone ID Related Accession (Same CDS sequence)	XM_004387610.1
Accession Version	XM_004387610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	RNA-binding protein EWS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444126.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGTAGA AGGGACCAAT 
ACAGGTTATA CTACTCCGGC TGCCCCCCAG GCATACAGCC AGCCTGTCCA GGGGTACGGC 
ACTGGTGCTT ATGATACCAC CACTGCTACG GTGACCACCA CCCAGGCCTC CTATGCAGCT 
CAGTCAGCAT ACGGCACTCA GCCTGCCTAC CCAGCATATG GGCAGCAGCC AGCAGCCACT 
GCACCTGCAA GACCGCAGGA TGGTAGCAAG CCTGCTGAGA CTAGTCAACC TCAGTCTAGC 
ACAGGAGGTT ACAACCAGCC CAGCATGGGG TATGGACAGA GTAACTACAG TTATCCCCAA 
GTACCTGGGA GCTACCCCAT GCAGCCAGTC ACCGCACCTC CATCCTATCC TCCTACCAGC 
TATTCCTCTT CACAGCCAAC TAGCTATGAT CAGAGCAGTT ACTCTCAGCA GAACACCTAT 
GGGCAGCCAA GCAGCTATGG ACAGCAGAGT AGCTATGGGC AACAGAGCAG CTATGGGCAG 
CAGCCGCCCA CTAGTTATCC CCCCCAAACT GGATCCTACA GCCAGGCTCC AAGTCAATAT 
AGCCAACAGA GCAGCAGCTA CGGGCAGCAG AGTTCATTCC GACAGGACCA CCCCAGTAGC 
ATGGGTGTTT ATGGTCAGGA GTCTGGAGGA TTTTCCGGAC CAGGAGAGAA CCGGAGCATG 
AGTGGCCCTG ATAACCGGGG CAGGGGAAGA GGGGGATTTG ATCGTGGAGG CATGAGCAGA 
GGTGGGCGGG GAGGAGGACG CGGTGGAATG GGCGCTGGAG AGCGAGGTGG CTTCAATAAG 
CCTGGTGGAC CCATGGACGA AGGACCAGAT CTTGATTTAG GCCCACCTGT AGATCCAGAT 
GAAGACTCTG ACAACAGTGC GATTTATGTG CAAGGATTAA ATGACAGTGT GACTCTAGAT 
GATCTGACAG ACTTCTTTAA GCAGTGTGGG GTTGTTAAGA TGAACAAGAG AACTGGGCAG 
CCCATGATCC ACATCTACCT GGACAAGGAA ACTGGGAAGC CTAAAGGCGA TGCCACAGTG 
TCCTATGAAG ACCCACCAAC TGCCAAGGCT GCCGTGGAGT GGTTTGATGG GAAAGATTTC 
CAAGGGAGCA AGCTTAAAGT CTCTCTTGCT CGGAAGAAGC CTCCTATGAA CAGCATGCGG 
GGCGGAATGC CACCCCGTGA GGGCAGAGGG ATGCCACCGC CACTCCGTGG AGGTCCGGGG 
GGCCCAGGAG GTCCTGGGGG ACCCATGGGT CGCATGGGAG GCCGTGGAGG AGACAGAGGA 
GGCTTCCCCC CAAGAGGACC CCGGGGTTCC CGAGGGAACC CATCTGGAGG AGGAAATGTC 
CAGCACCGAG CTGGAGACTG GCAGTGCCCC AACCCGGGTT GTGGAAACCA GAACTTCGCC 
TGGAGAACAG AATGCAACCA GTGTAAGGCC CCTAAGCCTG AAGGCTTCCT CCCGCCACCC 
TTCCCACCCC CGGGTGGTGA CCGTGGCAGA GGTGGCCCTG GTGGCATGCG GGGTGGGAGA 
GGTGGCCTCA TGGACCGCGG TGGTCCTGGT GGAATGTTCA GAGGTGGCCG TGGTGGAGAC 
AGAGGTGGCT TCCGTGGCGG CCGGGGCATG GACCGAGGTG GCTTTGGTGG AGGAAGACGA 
GGCGGTCCCG GAGGGCCCCC TGGACCTTTG ATGGAACAGA TGGGAGGAAG AAGAGGTGGA 
CGCGGAGGAC CTGGGAAGAT GGATAAAGGC GAGCACCGCC AGGAGCGCAG AGACCGGCCC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004387667.1
CDS	330..2315
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTNTGYTTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGSKPAETSQPQSSTGGYNQPSMGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSSQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLTDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	XM_004387610.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris EWS RNA-binding protein 1 (LOC101352929), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004387610.1

ORF Insert Sequence:

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGTAGA AGGGACCAAT 
ACAGGTTATA CTACTCCGGC TGCCCCCCAG GCATACAGCC AGCCTGTCCA GGGGTACGGC 
ACTGGTGCTT ATGATACCAC CACTGCTACG GTGACCACCA CCCAGGCCTC CTATGCAGCT 
CAGTCAGCAT ACGGCACTCA GCCTGCCTAC CCAGCATATG GGCAGCAGCC AGCAGCCACT 
GCACCTGCAA GACCGCAGGA TGGTAGCAAG CCTGCTGAGA CTAGTCAACC TCAGTCTAGC 
ACAGGAGGTT ACAACCAGCC CAGCATGGGG TATGGACAGA GTAACTACAG TTATCCCCAA 
GTACCTGGGA GCTACCCCAT GCAGCCAGTC ACCGCACCTC CATCCTATCC TCCTACCAGC 
TATTCCTCTT CACAGCCAAC TAGCTATGAT CAGAGCAGTT ACTCTCAGCA GAACACCTAT 
GGGCAGCCAA GCAGCTATGG ACAGCAGAGT AGCTATGGGC AACAGAGCAG CTATGGGCAG 
CAGCCGCCCA CTAGTTATCC CCCCCAAACT GGATCCTACA GCCAGGCTCC AAGTCAATAT 
AGCCAACAGA GCAGCAGCTA CGGGCAGCAG AGTTCATTCC GACAGGACCA CCCCAGTAGC 
ATGGGTGTTT ATGGTCAGGA GTCTGGAGGA TTTTCCGGAC CAGGAGAGAA CCGGAGCATG 
AGTGGCCCTG ATAACCGGGG CAGGGGAAGA GGGGGATTTG ATCGTGGAGG CATGAGCAGA 
GGTGGGCGGG GAGGAGGACG CGGTGGAATG GGCGCTGGAG AGCGAGGTGG CTTCAATAAG 
CCTGGTGGAC CCATGGACGA AGGACCAGAT CTTGATTTAG GCCCACCTGT AGATCCAGAT 
GAAGACTCTG ACAACAGTGC GATTTATGTG CAAGGATTAA ATGACAGTGT GACTCTAGAT 
GATCTGACAG ACTTCTTTAA GCAGTGTGGG GTTGTTAAGA TGAACAAGAG AACTGGGCAG 
CCCATGATCC ACATCTACCT GGACAAGGAA ACTGGGAAGC CTAAAGGCGA TGCCACAGTG 
TCCTATGAAG ACCCACCAAC TGCCAAGGCT GCCGTGGAGT GGTTTGATGG GAAAGATTTC 
CAAGGGAGCA AGCTTAAAGT CTCTCTTGCT CGGAAGAAGC CTCCTATGAA CAGCATGCGG 
GGCGGAATGC CACCCCGTGA GGGCAGAGGG ATGCCACCGC CACTCCGTGG AGGTCCGGGG 
GGCCCAGGAG GTCCTGGGGG ACCCATGGGT CGCATGGGAG GCCGTGGAGG AGACAGAGGA 
GGCTTCCCCC CAAGAGGACC CCGGGGTTCC CGAGGGAACC CATCTGGAGG AGGAAATGTC 
CAGCACCGAG CTGGAGACTG GCAGTGCCCC AACCCGGGTT GTGGAAACCA GAACTTCGCC 
TGGAGAACAG AATGCAACCA GTGTAAGGCC CCTAAGCCTG AAGGCTTCCT CCCGCCACCC 
TTCCCACCCC CGGGTGGTGA CCGTGGCAGA GGTGGCCCTG GTGGCATGCG GGGTGGGAGA 
GGTGGCCTCA TGGACCGCGG TGGTCCTGGT GGAATGTTCA GAGGTGGCCG TGGTGGAGAC 
AGAGGTGGCT TCCGTGGCGG CCGGGGCATG GACCGAGGTG GCTTTGGTGG AGGAAGACGA 
GGCGGTCCCG GAGGGCCCCC TGGACCTTTG ATGGAACAGA TGGGAGGAAG AAGAGGTGGA 
CGCGGAGGAC CTGGGAAGAT GGATAAAGGC GAGCACCGCC AGGAGCGCAG AGACCGGCCC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101352929 ( XM_004387611.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004387668.1 Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTr185783
Clone ID Related Accession (Same CDS sequence)	XM_004387611.1
Accession Version	XM_004387611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	RNA-binding protein EWS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444126.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGACCGCAG 
GATGGTAGCA AGCCTGCTGA GACTAGTCAA CCTCAGTCTA GCACAGGAGG TTACAACCAG 
CCCAGCATGG GGTATGGACA GAGTAACTAC AGTTATCCCC AAGTACCTGG GAGCTACCCC 
ATGCAGCCAG TCACCGCACC TCCATCCTAT CCTCCTACCA GCTATTCCTC TTCACAGCCA 
ACTAGCTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAGCC AAGCAGCTAT 
GGACAGCAGA GTAGCTATGG GCAACAGAGC AGCTATGGGC AGCAGCCGCC CACTAGTTAT 
CCCCCCCAAA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGTCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GACGAAGGAC CAGATCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCGATTT ATGTGCAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GACAGACTTC 
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAGCCCAT GATCCACATC 
TACCTGGACA AGGAAACTGG GAAGCCTAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA 
CCAACTGCCA AGGCTGCCGT GGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAGCTT 
AAAGTCTCTC TTGCTCGGAA GAAGCCTCCT ATGAACAGCA TGCGGGGCGG AATGCCACCC 
CGTGAGGGCA GAGGGATGCC ACCGCCACTC CGTGGAGGTC CGGGGGGCCC AGGAGGTCCT 
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGACA GAGGAGGCTT CCCCCCAAGA 
GGACCCCGGG GTTCCCGAGG GAACCCATCT GGAGGAGGAA ATGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGTTGTGGA AACCAGAACT TCGCCTGGAG AACAGAATGC 
AACCAGTGTA AGGCCCCTAA GCCTGAAGGC TTCCTCCCGC CACCCTTCCC ACCCCCGGGT 
GGTGACCGTG GCAGAGGTGG CCCTGGTGGC ATGCGGGGTG GGAGAGGTGG CCTCATGGAC 
CGCGGTGGTC CTGGTGGAAT GTTCAGAGGT GGCCGTGGTG GAGACAGAGG TGGCTTCCGT 
GGCGGCCGGG GCATGGACCG AGGTGGCTTT GGTGGAGGAA GACGAGGCGG TCCCGGAGGG 
CCCCCTGGAC CTTTGATGGA ACAGATGGGA GGAAGAAGAG GTGGACGCGG AGGACCTGGG 
AAGATGGATA AAGGCGAGCA CCGCCAGGAG CGCAGAGACC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004387668.1
CDS	330..2300
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGSKPAETSQPQSSTGGYNQPSMGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSSQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLTDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	XM_004387611.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004387611.1

ORF Insert Sequence:

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGACCGCAG 
GATGGTAGCA AGCCTGCTGA GACTAGTCAA CCTCAGTCTA GCACAGGAGG TTACAACCAG 
CCCAGCATGG GGTATGGACA GAGTAACTAC AGTTATCCCC AAGTACCTGG GAGCTACCCC 
ATGCAGCCAG TCACCGCACC TCCATCCTAT CCTCCTACCA GCTATTCCTC TTCACAGCCA 
ACTAGCTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAGCC AAGCAGCTAT 
GGACAGCAGA GTAGCTATGG GCAACAGAGC AGCTATGGGC AGCAGCCGCC CACTAGTTAT 
CCCCCCCAAA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGTCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GACGAAGGAC CAGATCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCGATTT ATGTGCAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GACAGACTTC 
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAGCCCAT GATCCACATC 
TACCTGGACA AGGAAACTGG GAAGCCTAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA 
CCAACTGCCA AGGCTGCCGT GGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAGCTT 
AAAGTCTCTC TTGCTCGGAA GAAGCCTCCT ATGAACAGCA TGCGGGGCGG AATGCCACCC 
CGTGAGGGCA GAGGGATGCC ACCGCCACTC CGTGGAGGTC CGGGGGGCCC AGGAGGTCCT 
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGACA GAGGAGGCTT CCCCCCAAGA 
GGACCCCGGG GTTCCCGAGG GAACCCATCT GGAGGAGGAA ATGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGTTGTGGA AACCAGAACT TCGCCTGGAG AACAGAATGC 
AACCAGTGTA AGGCCCCTAA GCCTGAAGGC TTCCTCCCGC CACCCTTCCC ACCCCCGGGT 
GGTGACCGTG GCAGAGGTGG CCCTGGTGGC ATGCGGGGTG GGAGAGGTGG CCTCATGGAC 
CGCGGTGGTC CTGGTGGAAT GTTCAGAGGT GGCCGTGGTG GAGACAGAGG TGGCTTCCGT 
GGCGGCCGGG GCATGGACCG AGGTGGCTTT GGTGGAGGAA GACGAGGCGG TCCCGGAGGG 
CCCCCTGGAC CTTTGATGGA ACAGATGGGA GGAAGAAGAG GTGGACGCGG AGGACCTGGG 
AAGATGGATA AAGGCGAGCA CCGCCAGGAG CGCAGAGACC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101352929 ( XM_012558847.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012414301.1 Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTr185784
Clone ID Related Accession (Same CDS sequence)	XM_012558847.1
Accession Version	XM_012558847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	RNA-binding protein EWS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444126.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGACCGCAG 
GATGGTAGCA AGCCTGCTGA GACTAGTCAA CCTCAGTCTA GCACAGGAGG TTACAACCAG 
CCCAGCATGG GGTATGGACA GAGTAACTAC AGTTATCCCC AAGTACCTGG GAGCTACCCC 
ATGCAGCCAG TCACCGCACC TCCATCCTAT CCTCCTACCA GCTATTCCTC TTCACAGCCA 
ACTAGCTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAGCC AAGCAGCTAT 
GGACAGCAGA GTAGCTATGG GCAACAGAGC AGCTATGGGC AGCAGCCGCC CACTAGTTAT 
CCCCCCCAAA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGTCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCGCTGG AGAGCGAGGT GGCTTCAATA AGCCTGGTGG ACCCATGGAC 
GAAGGACCAG ATCTTGATTT AGGCCCACCT GTAGATCCAG ATGAAGACTC TGACAACAGT 
GCGATTTATG TGCAAGGATT AAATGACAGT GTGACTCTAG ATGATCTGAC AGACTTCTTT 
AAGCAGTGTG GGGTTGTTAA GATGAACAAG AGAACTGGGC AGCCCATGAT CCACATCTAC 
CTGGACAAGG AAACTGGGAA GCCTAAAGGC GATGCCACAG TGTCCTATGA AGACCCACCA 
ACTGCCAAGG CTGCCGTGGA GTGGTTTGAT GGGAAAGATT TCCAAGGGAG CAAGCTTAAA 
GTCTCTCTTG CTCGGAAGAA GCCTCCTATG AACAGCATGC GGGGCGGAAT GCCACCCCGT 
GAGGGCAGAG GGATGCCACC GCCACTCCGT GGAGGTCCGG GGGGCCCAGG AGGTCCTGGG 
GGACCCATGG GTCGCATGGG AGGCCGTGGA GGAGACAGAG GAGGCTTCCC CCCAAGAGGA 
CCCCGGGGTT CCCGAGGGAA CCCATCTGGA GGAGGAAATG TCCAGCACCG AGCTGGAGAC 
TGGCAGTGCC CCAACCCGGG TTGTGGAAAC CAGAACTTCG CCTGGAGAAC AGAATGCAAC 
CAGTGTAAGG CCCCTAAGCC TGAAGGCTTC CTCCCGCCAC CCTTCCCACC CCCGGGTGGT 
GACCGTGGCA GAGGTGGCCC TGGTGGCATG CGGGGTGGGA GAGGTGGCCT CATGGACCGC 
GGTGGTCCTG GTGGAATGTT CAGAGGTGGC CGTGGTGGAG ACAGAGGTGG CTTCCGTGGC 
GGCCGGGGCA TGGACCGAGG TGGCTTTGGT GGAGGAAGAC GAGGCGGTCC CGGAGGGCCC 
CCTGGACCTT TGATGGAACA GATGGGAGGA AGAAGAGGTG GACGCGGAGG ACCTGGGAAG 
ATGGATAAAG GCGAGCACCG CCAGGAGCGC AGAGACCGGC CCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012414301.1
CDS	330..2297
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGSKPAETSQPQSSTGGYNQPSMGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSSQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLTDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	XM_012558847.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012558847.1

ORF Insert Sequence:

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGACCGCAG 
GATGGTAGCA AGCCTGCTGA GACTAGTCAA CCTCAGTCTA GCACAGGAGG TTACAACCAG 
CCCAGCATGG GGTATGGACA GAGTAACTAC AGTTATCCCC AAGTACCTGG GAGCTACCCC 
ATGCAGCCAG TCACCGCACC TCCATCCTAT CCTCCTACCA GCTATTCCTC TTCACAGCCA 
ACTAGCTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAGCC AAGCAGCTAT 
GGACAGCAGA GTAGCTATGG GCAACAGAGC AGCTATGGGC AGCAGCCGCC CACTAGTTAT 
CCCCCCCAAA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGTCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCGCTGG AGAGCGAGGT GGCTTCAATA AGCCTGGTGG ACCCATGGAC 
GAAGGACCAG ATCTTGATTT AGGCCCACCT GTAGATCCAG ATGAAGACTC TGACAACAGT 
GCGATTTATG TGCAAGGATT AAATGACAGT GTGACTCTAG ATGATCTGAC AGACTTCTTT 
AAGCAGTGTG GGGTTGTTAA GATGAACAAG AGAACTGGGC AGCCCATGAT CCACATCTAC 
CTGGACAAGG AAACTGGGAA GCCTAAAGGC GATGCCACAG TGTCCTATGA AGACCCACCA 
ACTGCCAAGG CTGCCGTGGA GTGGTTTGAT GGGAAAGATT TCCAAGGGAG CAAGCTTAAA 
GTCTCTCTTG CTCGGAAGAA GCCTCCTATG AACAGCATGC GGGGCGGAAT GCCACCCCGT 
GAGGGCAGAG GGATGCCACC GCCACTCCGT GGAGGTCCGG GGGGCCCAGG AGGTCCTGGG 
GGACCCATGG GTCGCATGGG AGGCCGTGGA GGAGACAGAG GAGGCTTCCC CCCAAGAGGA 
CCCCGGGGTT CCCGAGGGAA CCCATCTGGA GGAGGAAATG TCCAGCACCG AGCTGGAGAC 
TGGCAGTGCC CCAACCCGGG TTGTGGAAAC CAGAACTTCG CCTGGAGAAC AGAATGCAAC 
CAGTGTAAGG CCCCTAAGCC TGAAGGCTTC CTCCCGCCAC CCTTCCCACC CCCGGGTGGT 
GACCGTGGCA GAGGTGGCCC TGGTGGCATG CGGGGTGGGA GAGGTGGCCT CATGGACCGC 
GGTGGTCCTG GTGGAATGTT CAGAGGTGGC CGTGGTGGAG ACAGAGGTGG CTTCCGTGGC 
GGCCGGGGCA TGGACCGAGG TGGCTTTGGT GGAGGAAGAC GAGGCGGTCC CGGAGGGCCC 
CCTGGACCTT TGATGGAACA GATGGGAGGA AGAAGAGGTG GACGCGGAGG ACCTGGGAAG 
ATGGATAAAG GCGAGCACCG CCAGGAGCGC AGAGACCGGC CCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101352929 ( XM_004387612.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004387669.1 Trichechus manatus latirostris EWS RNA-binding protein 1 (LOC101352929), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTr185785
Clone ID Related Accession (Same CDS sequence)	XM_004387612.1
Accession Version	XM_004387612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	RNA-binding protein EWS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444126.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGCTATTCC 
TCTTCACAGC CAACTAGCTA TGATCAGAGC AGTTACTCTC AGCAGAACAC CTATGGGCAG 
CCAAGCAGCT ATGGACAGCA GAGTAGCTAT GGGCAACAGA GCAGCTATGG GCAGCAGCCG 
CCCACTAGTT ATCCCCCCCA AACTGGATCC TACAGCCAGG CTCCAAGTCA ATATAGCCAA 
CAGAGCAGCA GCTACGGGCA GCAGAGTTCA TTCCGACAGG ACCACCCCAG TAGCATGGGT 
GTTTATGGTC AGGAGTCTGG AGGATTTTCC GGACCAGGAG AGAACCGGAG CATGAGTGGC 
CCTGATAACC GGGGCAGGGG AAGAGGGGGA TTTGATCGTG GAGGCATGAG CAGAGGTGGG 
CGGGGAGGAG GACGCGGTGG AATGGGCAGC GCTGGAGAGC GAGGTGGCTT CAATAAGCCT 
GGTGGACCCA TGGACGAAGG ACCAGATCTT GATTTAGGCC CACCTGTAGA TCCAGATGAA 
GACTCTGACA ACAGTGCGAT TTATGTGCAA GGATTAAATG ACAGTGTGAC TCTAGATGAT 
CTGACAGACT TCTTTAAGCA GTGTGGGGTT GTTAAGATGA ACAAGAGAAC TGGGCAGCCC 
ATGATCCACA TCTACCTGGA CAAGGAAACT GGGAAGCCTA AAGGCGATGC CACAGTGTCC 
TATGAAGACC CACCAACTGC CAAGGCTGCC GTGGAGTGGT TTGATGGGAA AGATTTCCAA 
GGGAGCAAGC TTAAAGTCTC TCTTGCTCGG AAGAAGCCTC CTATGAACAG CATGCGGGGC 
GGAATGCCAC CCCGTGAGGG CAGAGGGATG CCACCGCCAC TCCGTGGAGG TCCGGGGGGC 
CCAGGAGGTC CTGGGGGACC CATGGGTCGC ATGGGAGGCC GTGGAGGAGA CAGAGGAGGC 
TTCCCCCCAA GAGGACCCCG GGGTTCCCGA GGGAACCCAT CTGGAGGAGG AAATGTCCAG 
CACCGAGCTG GAGACTGGCA GTGCCCCAAC CCGGGTTGTG GAAACCAGAA CTTCGCCTGG 
AGAACAGAAT GCAACCAGTG TAAGGCCCCT AAGCCTGAAG GCTTCCTCCC GCCACCCTTC 
CCACCCCCGG GTGGTGACCG TGGCAGAGGT GGCCCTGGTG GCATGCGGGG TGGGAGAGGT 
GGCCTCATGG ACCGCGGTGG TCCTGGTGGA ATGTTCAGAG GTGGCCGTGG TGGAGACAGA 
GGTGGCTTCC GTGGCGGCCG GGGCATGGAC CGAGGTGGCT TTGGTGGAGG AAGACGAGGC 
GGTCCCGGAG GGCCCCCTGG ACCTTTGATG GAACAGATGG GAGGAAGAAG AGGTGGACGC 
GGAGGACCTG GGAAGATGGA TAAAGGCGAG CACCGCCAGG AGCGCAGAGA CCGGCCCTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004387669.1
CDS	330..2132
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPASYSSSQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLTDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	XM_004387612.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris EWS RNA-binding protein 1 (LOC101352929), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004387612.1

ORF Insert Sequence:

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGCTATTCC 
TCTTCACAGC CAACTAGCTA TGATCAGAGC AGTTACTCTC AGCAGAACAC CTATGGGCAG 
CCAAGCAGCT ATGGACAGCA GAGTAGCTAT GGGCAACAGA GCAGCTATGG GCAGCAGCCG 
CCCACTAGTT ATCCCCCCCA AACTGGATCC TACAGCCAGG CTCCAAGTCA ATATAGCCAA 
CAGAGCAGCA GCTACGGGCA GCAGAGTTCA TTCCGACAGG ACCACCCCAG TAGCATGGGT 
GTTTATGGTC AGGAGTCTGG AGGATTTTCC GGACCAGGAG AGAACCGGAG CATGAGTGGC 
CCTGATAACC GGGGCAGGGG AAGAGGGGGA TTTGATCGTG GAGGCATGAG CAGAGGTGGG 
CGGGGAGGAG GACGCGGTGG AATGGGCAGC GCTGGAGAGC GAGGTGGCTT CAATAAGCCT 
GGTGGACCCA TGGACGAAGG ACCAGATCTT GATTTAGGCC CACCTGTAGA TCCAGATGAA 
GACTCTGACA ACAGTGCGAT TTATGTGCAA GGATTAAATG ACAGTGTGAC TCTAGATGAT 
CTGACAGACT TCTTTAAGCA GTGTGGGGTT GTTAAGATGA ACAAGAGAAC TGGGCAGCCC 
ATGATCCACA TCTACCTGGA CAAGGAAACT GGGAAGCCTA AAGGCGATGC CACAGTGTCC 
TATGAAGACC CACCAACTGC CAAGGCTGCC GTGGAGTGGT TTGATGGGAA AGATTTCCAA 
GGGAGCAAGC TTAAAGTCTC TCTTGCTCGG AAGAAGCCTC CTATGAACAG CATGCGGGGC 
GGAATGCCAC CCCGTGAGGG CAGAGGGATG CCACCGCCAC TCCGTGGAGG TCCGGGGGGC 
CCAGGAGGTC CTGGGGGACC CATGGGTCGC ATGGGAGGCC GTGGAGGAGA CAGAGGAGGC 
TTCCCCCCAA GAGGACCCCG GGGTTCCCGA GGGAACCCAT CTGGAGGAGG AAATGTCCAG 
CACCGAGCTG GAGACTGGCA GTGCCCCAAC CCGGGTTGTG GAAACCAGAA CTTCGCCTGG 
AGAACAGAAT GCAACCAGTG TAAGGCCCCT AAGCCTGAAG GCTTCCTCCC GCCACCCTTC 
CCACCCCCGG GTGGTGACCG TGGCAGAGGT GGCCCTGGTG GCATGCGGGG TGGGAGAGGT 
GGCCTCATGG ACCGCGGTGG TCCTGGTGGA ATGTTCAGAG GTGGCCGTGG TGGAGACAGA 
GGTGGCTTCC GTGGCGGCCG GGGCATGGAC CGAGGTGGCT TTGGTGGAGG AAGACGAGGC 
GGTCCCGGAG GGCCCCCTGG ACCTTTGATG GAACAGATGG GAGGAAGAAG AGGTGGACGC 
GGAGGACCTG GGAAGATGGA TAAAGGCGAG CACCGCCAGG AGCGCAGAGA CCGGCCCTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101352929 ( XM_004387613.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004387670.1 Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X4, mRNA.

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CloneID	OTr185786
Clone ID Related Accession (Same CDS sequence)	XM_004387613.2 , XM_004387613.1
Accession Version	XM_004387613.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	RNA-binding protein EWS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444126.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_004387613.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGACCGCAG 
GATGGTAGCA AGCCTGCTGA GACTAGTCAA CCTCAGTCTA GCACAGGAGG TTACAACCAG 
CCCAGCATGG GGTATGGACA GAGTAACTAC AGTTATCCCC AAGTACCTGG GAGCTACCCC 
ATGCAGCCAG TCACCGCACC TCCATCCTAT CCTCCTACCA GCTATTCCTC TTCACAGCCA 
ACTAGCTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAGCC AAGCAGCTAT 
GGACAGCAGA GTAGCTATGG GCAACAGAGC AGCTATGGGC AGCAGCCGCC CACTAGTTAT 
CCCCCCCAAA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGTCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGGTTACA AAGTGAGAGC CTTGTATACA CTTCAATACT TAAAAAGTAC 
CCGTACTCAG TACTCAGCCG GCAGCATAAT GAAAAGTGGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004387670.1
CDS	330..1394
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGSKPAETSQPQSSTGGYNQPSMGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSSQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGLQSESLVYTSILKKYPYSVLSRQHNEKWD

Target ORF information:

RefSeq Version	XM_004387613.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris EWS RNA binding protein 1 (LOC101352929), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004387613.2

ORF Insert Sequence:

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGACCGCAG 
GATGGTAGCA AGCCTGCTGA GACTAGTCAA CCTCAGTCTA GCACAGGAGG TTACAACCAG 
CCCAGCATGG GGTATGGACA GAGTAACTAC AGTTATCCCC AAGTACCTGG GAGCTACCCC 
ATGCAGCCAG TCACCGCACC TCCATCCTAT CCTCCTACCA GCTATTCCTC TTCACAGCCA 
ACTAGCTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAGCC AAGCAGCTAT 
GGACAGCAGA GTAGCTATGG GCAACAGAGC AGCTATGGGC AGCAGCCGCC CACTAGTTAT 
CCCCCCCAAA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGTCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGGTTACA AAGTGAGAGC CTTGTATACA CTTCAATACT TAAAAAGTAC 
CCGTACTCAG TACTCAGCCG GCAGCATAAT GAAAAGTGGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101352929 ( XM_004387613.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004387670.1 Trichechus manatus latirostris EWS RNA-binding protein 1 (LOC101352929), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTr185786
Clone ID Related Accession (Same CDS sequence)	XM_004387613.2 , XM_004387613.1
Accession Version	XM_004387613.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	RNA-binding protein EWS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444126.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGACCGCAG 
GATGGTAGCA AGCCTGCTGA GACTAGTCAA CCTCAGTCTA GCACAGGAGG TTACAACCAG 
CCCAGCATGG GGTATGGACA GAGTAACTAC AGTTATCCCC AAGTACCTGG GAGCTACCCC 
ATGCAGCCAG TCACCGCACC TCCATCCTAT CCTCCTACCA GCTATTCCTC TTCACAGCCA 
ACTAGCTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAGCC AAGCAGCTAT 
GGACAGCAGA GTAGCTATGG GCAACAGAGC AGCTATGGGC AGCAGCCGCC CACTAGTTAT 
CCCCCCCAAA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGTCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGGTTACA AAGTGAGAGC CTTGTATACA CTTCAATACT TAAAAAGTAC 
CCGTACTCAG TACTCAGCCG GCAGCATAAT GAAAAGTGGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004387670.1
CDS	330..1394
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGSKPAETSQPQSSTGGYNQPSMGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSSQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGLQSESLVYTSILKKYPYSVLSRQHNEKWD

Target ORF information:

RefSeq Version	XM_004387613.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris EWS RNA-binding protein 1 (LOC101352929), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004387613.1

ORF Insert Sequence:

ATGGCGTCCA CGGATTACAG TACCTACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CTACTGGTTA TACTACTCCG 
GCTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGATACC 
ACCACTGCTA CGGTGACCAC CACCCAGGCC TCCTATGCAG CTCAGTCAGC ATACGGCACT 
CAGCCTGCCT ACCCAGCATA TGGGCAGCAG CCAGCAGCCA CTGCACCTGC AAGACCGCAG 
GATGGTAGCA AGCCTGCTGA GACTAGTCAA CCTCAGTCTA GCACAGGAGG TTACAACCAG 
CCCAGCATGG GGTATGGACA GAGTAACTAC AGTTATCCCC AAGTACCTGG GAGCTACCCC 
ATGCAGCCAG TCACCGCACC TCCATCCTAT CCTCCTACCA GCTATTCCTC TTCACAGCCA 
ACTAGCTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAGCC AAGCAGCTAT 
GGACAGCAGA GTAGCTATGG GCAACAGAGC AGCTATGGGC AGCAGCCGCC CACTAGTTAT 
CCCCCCCAAA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGTCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGGTTACA AAGTGAGAGC CTTGTATACA CTTCAATACT TAAAAAGTAC 
CCGTACTCAG TACTCAGCCG GCAGCATAAT GAAAAGTGGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101352929 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム