LOC101350025 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101350025
Entrez Gene ID	101350025
Full Name	La ribonucleoprotein domain family member 1
Synonyms	LARP1
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012560139.1	XP_012415593.1	la-related protein 1
XM_012560139.2	XP_012415593.1	la-related protein 1

Pathways from BioSystems
Gene Ontology

The following LOC101350025 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101350025 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr167469	XM_012560139.2 Latest version!	Trichechus manatus latirostris La ribonucleoprotein domain family member 1 (LOC101350025), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OTr167469	XM_012560139.1	Trichechus manatus latirostris La ribonucleoprotein domain family, member 1 (LOC101350025), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101350025 ( XM_012560139.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012415593.1 Trichechus manatus latirostris La ribonucleoprotein domain family, member 1 (LOC101350025), mRNA.

Price & Availability↑

CloneID	OTr167469
Clone ID Related Accession (Same CDS sequence)	XM_012560139.1 , XM_012560139.2
Accession Version	XM_012560139.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	la-related protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443947.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTC AGGTGGAGCC GCTGCTGCCG GGGGGCGCCC CGCTCTTGCA GGCCGAAGAG 
CACGGGGGGC TGGTGAGGAA GAAGCTGCCG CCGCCGCCCG AGGGCAAGGG CGAGCCTGGG 
GCCAACGACG TCGGAGTCGG GGAGCCGGAC GGCGGCACTC GGAGACCCCG GCTGCCCTGC 
GCCAAGCCGT ACAAGGAGGG CACCGGGCAA CAGGAGCGCG AGAGTCCGCG GCCGCCGCAG 
CCGCCGCAGC CGCCAGGCGC CGAAGGCCCG GCCGTCAGCG ACGGGGAGGA GGGCGGCGGC 
GGCGAGCCGG GCGCTGGCGG AGGAGCAACC GGAGCTGCGG GGGGCGGCGG GCGGCGAGAC 
TTCGTGGAGG CCCCCCCGCC CAAGGTGAAC CCGTGGACTA AGAACGCGTT GCCGCAGGCC 
CTGGCCACGG TGAACGGACA GTCCCCTCCA GAACCTTCTG CCCCAACCAA GGTTGTGAGG 
GCAGCAATTC CTAAACAGCG CAAAGGCAGC AAGGTTGGTG ACTTTGGAGA TGCAATCAAC 
TGGCCCACAC CAGGAGAAAT AGCCCACAAG AGTGTACAGC CACAGCCCCA CAAGTCTCAG 
CCTGCCCGTA AGCCACCACC CAAGAAGGAC ATGAGGGAAC AGGAGAAAGG AGAGGGGAGT 
GATAGTAAGG AGAGCCCAAA AACCAAGTCA GATGAATCAG GGGAGGAGAA GAATGGGGAC 
GAAGACTGTC AACGAGGAGG GCAGAAGAAG AAAGGGAACA AACACAAATG GGTTCCATTG 
CAAATAGACA TGAAGCCTGA AGCACCCAGA GAGAAACTGA CCTCGCGCCC CTCTCGCCCA 
CCAGATCCTC GTCACACACC TGCCAACCGC GGGGAGATCA AAGGGTCTGA GCCTGCTACC 
TACGTGCCCG TGCCCGTGGC CCCCCCCACC CCAGCCTGGC AACCAGAGAC CAAACCGGAG 
CCTGCCTGGC ATGACCAGGA TGAGACATCG AGTGTGAAGA GTGATGGGGC TGGTGGGGCG 
CGGGCTTCCT TCCGTGGCCG TGGACGGGGG CGTGGTCGCG GCCGGGGACG CGGCCGGGGT 
GGCACTCGAA CCCATTTCGA CTACCAGTTT GGCTACCGAA AGTTTGATGC TGCAGAGGGG 
CCTCGCATCC CCAAGTATAT GAACAACATC ACCTACTATT TTGACAATGT GAGCAGCACT 
GAGCTTTACA GCGTGGACCA GGAGCTGCTC AAAGACTACA TCAAGCGCCA GATTGAATAC 
TACTTCAGCG TGGACAATTT AGAGCGAGAC TTCTTCCTGC GACGAAAAAT GGATGCCAGC 
GGTTTCCTTC CCATCACCCT TATTGCTTCC TTCCACCGAG TGCAGGCCCT TACCACTGAC 
ATTTCACTCA TCTTTGCGGC CCTCAAGGAC AGCAAGGTGG TGGAGATAGT TGATGAGAAG 
GTCCGCAGGA GGGAGGAGCC TGAGAAGTGG CCTCTTCCTG GTCCCCCAAT GGTGGACTAT 
TCACAAACCG ATTTCTCCCA GCTTCTCAAC TGCCCCGAGT TTGTTCCTCG CCAGCACTAT 
CAGAAGGAGA CAGAATCGGC CCCTGGCTCT CCTCGGGCAG TCACTCCAGT GCCAACCAAA 
ACCGAGGAGG TCAGCAACCT AAAGACGCTG CCCAAGGGCC TGTCTGCCAG TCTGCCTGAC 
CTGGATTCTG AGAACTGGAT TGAAGTAAAG AAGAGGCCTC GTCCCTCCCC AGCACGGCCC 
AAGAAGTCAG AGGAGCCCAG GTTCCTCCAC CAGACCTCTC TGCCTCAGCA GCTGCCCTCT 
CAGCAGCTGA TGTCCAAGGA TCAGGATGAG CAAGAGGAAC TAGATTTTCT GTTTGATGAG 
GAGATGGAGC AGATGGATGG ACGGAAGAAC ACCTTTACCG CCTGGTCTGA TGAGGACTCT 
GACTATGAGA TTGACGACCG GGACGTCAAC AAGATCCTCA TTGTCACCCA GACGCCACCT 
TACATGCGCC GGCACCCAGG GGGGGACCGC ACAGGCAACC ACACCTCACG TGCCAAGATG 
AGCGCTGAGC TGGCCAAGGT CATTAACGAT GGGCTCTTCT ACTATGAGCA GGACCTGTGG 
ACTGAGAAGT TTGAACCTGA GTATTCCCAG ATCAAGCAAG AAGTTGAGAA CTTCAAGAAA 
GTCAACATGA TCAGCCGGGA GCAGTTTGAC ACTCTGACCC CCGAACCCCC TGTGGATCCC 
AACCAGGAAG TCCCTCCTGG GCCACCCCGG TTCCAGCAAG TTCCCACTGA TGCCCTGGCC 
AACAAGTTGT TTGGTGCTCC TGAGCCTTCC ACTATCGCCC GCTCTCTACC AACCACTGTC 
CCAGAGTCGC CAAACTACCG TAATGCCAGG ACTCCCCGGA CACCCCGCAC ACCGCAGCTC 
AAAGACTCAA GCCAGACATC ACGATTTTAC CCGGTGGTGA AGGAAGGACG GACAATAGAT 
GCCAAGATGC CTCGGAAAAG GAAGACAAGA CACAGTTCAA ACCCGCCATT GGAGAGTCAT 
GTGGGCTGGG TTATGGATTC TCGTGAGCAC AGGCCCCGGA CTGCTTCCAT CAGCTCCAGC 
CCCTCGGAAG GAACACCTGC AGTTGGCAGC TATGGCTGTA CCCCTCAGTC ATTGCCCAAG 
TTCCAGCATC CTTCCCATGA GCTGCTAAAG GAAAATGGCT TCACACAACA CGTCTACCAT 
AAGTATCGTA GGCGCTGCCT TAACGAGCGG AAACGCTTGG GCATTGGCCA ATCTCAGGAG 
ATGAACACTC TCTTCCGCTT CTGGTCCTTC TTCCTCCGAG ATCACTTCAA CAAAAAGATG 
TATGAGGAGT TCAAGCAGCT GGCTCTGGAG GATGCTAAAG AAGGCTACAG ATATGGTTTG 
GAGTGCCTTT TTCGATACTA CAGTTATGGC CTGGAAAAGA AGTTCCGCTT GGACATATTC 
AAGGATTTTC AGGAGGAAAC CGTGAAGGAC TACGAAGCTG GCCAACTCTA TGGGCTGGAG 
AAGTTCTGGG CCTTCCTGAA ATATTCCAAA GCCAAAAATT TGGACATTGA CCCCAAATTG 
CAAGAATACC TCGGCAAATT CCGACGTCTT GAAGACTTCC GAGTAGATCC CCCAATGGGT 
GAGGAGGGCA ACCACAAGCG ACACCCAGTG GCAGCAGGAG GTGGCGGCGG TGAGAGCAGA 
AAGAGGTGCC CTTCCCAGTC TTCCAGCAGG CCCACCACCA TGAACAGCCA ACCCCTCACG 
CCCCCCTCCG GCCAGTCCAT CCGGGAAGAT GCCAAATGGA CAAGCCAGCA CTCGGACATA 
CAGACTTTGG GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012415593.1
CDS	180..3497
Translation	MATQVEPLLPGGAPLLQAEEHGGLVRKKLPPPPEGKGEPGANDVGVGEPDGGTRRPRLPCAKPYKEGTGQQERESPRPPQPPQPPGAEGPAVSDGEEGGGGEPGAGGGATGAAGGGGRRDFVEAPPPKVNPWTKNALPQALATVNGQSPPEPSAPTKVVRAAIPKQRKGSKVGDFGDAINWPTPGEIAHKSVQPQPHKSQPARKPPPKKDMREQEKGEGSDSKESPKTKSDESGEEKNGDEDCQRGGQKKKGNKHKWVPLQIDMKPEAPREKLTSRPSRPPDPRHTPANRGEIKGSEPATYVPVPVAPPTPAWQPETKPEPAWHDQDETSSVKSDGAGGARASFRGRGRGRGRGRGRGRGGTRTHFDYQFGYRKFDAAEGPRIPKYMNNITYYFDNVSSTELYSVDQELLKDYIKRQIEYYFSVDNLERDFFLRRKMDASGFLPITLIASFHRVQALTTDISLIFAALKDSKVVEIVDEKVRRREEPEKWPLPGPPMVDYSQTDFSQLLNCPEFVPRQHYQKETESAPGSPRAVTPVPTKTEEVSNLKTLPKGLSASLPDLDSENWIEVKKRPRPSPARPKKSEEPRFLHQTSLPQQLPSQQLMSKDQDEQEELDFLFDEEMEQMDGRKNTFTAWSDEDSDYEIDDRDVNKILIVTQTPPYMRRHPGGDRTGNHTSRAKMSAELAKVINDGLFYYEQDLWTEKFEPEYSQIKQEVENFKKVNMISREQFDTLTPEPPVDPNQEVPPGPPRFQQVPTDALANKLFGAPEPSTIARSLPTTVPESPNYRNARTPRTPRTPQLKDSSQTSRFYPVVKEGRTIDAKMPRKRKTRHSSNPPLESHVGWVMDSREHRPRTASISSSPSEGTPAVGSYGCTPQSLPKFQHPSHELLKENGFTQHVYHKYRRRCLNERKRLGIGQSQEMNTLFRFWSFFLRDHFNKKMYEEFKQLALEDAKEGYRYGLECLFRYYSYGLEKKFRLDIFKDFQEETVKDYEAGQLYGLEKFWAFLKYSKAKNLDIDPKLQEYLGKFRRLEDFRVDPPMGEEGNHKRHPVAAGGGGGESRKRCPSQSSSRPTTMNSQPLTPPSGQSIREDAKWTSQHSDIQTLGK

Target ORF information:

RefSeq Version	XM_012560139.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris La ribonucleoprotein domain family, member 1 (LOC101350025), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012560139.1

ORF Insert Sequence:

ATGGCCACTC AGGTGGAGCC GCTGCTGCCG GGGGGCGCCC CGCTCTTGCA GGCCGAAGAG 
CACGGGGGGC TGGTGAGGAA GAAGCTGCCG CCGCCGCCCG AGGGCAAGGG CGAGCCTGGG 
GCCAACGACG TCGGAGTCGG GGAGCCGGAC GGCGGCACTC GGAGACCCCG GCTGCCCTGC 
GCCAAGCCGT ACAAGGAGGG CACCGGGCAA CAGGAGCGCG AGAGTCCGCG GCCGCCGCAG 
CCGCCGCAGC CGCCAGGCGC CGAAGGCCCG GCCGTCAGCG ACGGGGAGGA GGGCGGCGGC 
GGCGAGCCGG GCGCTGGCGG AGGAGCAACC GGAGCTGCGG GGGGCGGCGG GCGGCGAGAC 
TTCGTGGAGG CCCCCCCGCC CAAGGTGAAC CCGTGGACTA AGAACGCGTT GCCGCAGGCC 
CTGGCCACGG TGAACGGACA GTCCCCTCCA GAACCTTCTG CCCCAACCAA GGTTGTGAGG 
GCAGCAATTC CTAAACAGCG CAAAGGCAGC AAGGTTGGTG ACTTTGGAGA TGCAATCAAC 
TGGCCCACAC CAGGAGAAAT AGCCCACAAG AGTGTACAGC CACAGCCCCA CAAGTCTCAG 
CCTGCCCGTA AGCCACCACC CAAGAAGGAC ATGAGGGAAC AGGAGAAAGG AGAGGGGAGT 
GATAGTAAGG AGAGCCCAAA AACCAAGTCA GATGAATCAG GGGAGGAGAA GAATGGGGAC 
GAAGACTGTC AACGAGGAGG GCAGAAGAAG AAAGGGAACA AACACAAATG GGTTCCATTG 
CAAATAGACA TGAAGCCTGA AGCACCCAGA GAGAAACTGA CCTCGCGCCC CTCTCGCCCA 
CCAGATCCTC GTCACACACC TGCCAACCGC GGGGAGATCA AAGGGTCTGA GCCTGCTACC 
TACGTGCCCG TGCCCGTGGC CCCCCCCACC CCAGCCTGGC AACCAGAGAC CAAACCGGAG 
CCTGCCTGGC ATGACCAGGA TGAGACATCG AGTGTGAAGA GTGATGGGGC TGGTGGGGCG 
CGGGCTTCCT TCCGTGGCCG TGGACGGGGG CGTGGTCGCG GCCGGGGACG CGGCCGGGGT 
GGCACTCGAA CCCATTTCGA CTACCAGTTT GGCTACCGAA AGTTTGATGC TGCAGAGGGG 
CCTCGCATCC CCAAGTATAT GAACAACATC ACCTACTATT TTGACAATGT GAGCAGCACT 
GAGCTTTACA GCGTGGACCA GGAGCTGCTC AAAGACTACA TCAAGCGCCA GATTGAATAC 
TACTTCAGCG TGGACAATTT AGAGCGAGAC TTCTTCCTGC GACGAAAAAT GGATGCCAGC 
GGTTTCCTTC CCATCACCCT TATTGCTTCC TTCCACCGAG TGCAGGCCCT TACCACTGAC 
ATTTCACTCA TCTTTGCGGC CCTCAAGGAC AGCAAGGTGG TGGAGATAGT TGATGAGAAG 
GTCCGCAGGA GGGAGGAGCC TGAGAAGTGG CCTCTTCCTG GTCCCCCAAT GGTGGACTAT 
TCACAAACCG ATTTCTCCCA GCTTCTCAAC TGCCCCGAGT TTGTTCCTCG CCAGCACTAT 
CAGAAGGAGA CAGAATCGGC CCCTGGCTCT CCTCGGGCAG TCACTCCAGT GCCAACCAAA 
ACCGAGGAGG TCAGCAACCT AAAGACGCTG CCCAAGGGCC TGTCTGCCAG TCTGCCTGAC 
CTGGATTCTG AGAACTGGAT TGAAGTAAAG AAGAGGCCTC GTCCCTCCCC AGCACGGCCC 
AAGAAGTCAG AGGAGCCCAG GTTCCTCCAC CAGACCTCTC TGCCTCAGCA GCTGCCCTCT 
CAGCAGCTGA TGTCCAAGGA TCAGGATGAG CAAGAGGAAC TAGATTTTCT GTTTGATGAG 
GAGATGGAGC AGATGGATGG ACGGAAGAAC ACCTTTACCG CCTGGTCTGA TGAGGACTCT 
GACTATGAGA TTGACGACCG GGACGTCAAC AAGATCCTCA TTGTCACCCA GACGCCACCT 
TACATGCGCC GGCACCCAGG GGGGGACCGC ACAGGCAACC ACACCTCACG TGCCAAGATG 
AGCGCTGAGC TGGCCAAGGT CATTAACGAT GGGCTCTTCT ACTATGAGCA GGACCTGTGG 
ACTGAGAAGT TTGAACCTGA GTATTCCCAG ATCAAGCAAG AAGTTGAGAA CTTCAAGAAA 
GTCAACATGA TCAGCCGGGA GCAGTTTGAC ACTCTGACCC CCGAACCCCC TGTGGATCCC 
AACCAGGAAG TCCCTCCTGG GCCACCCCGG TTCCAGCAAG TTCCCACTGA TGCCCTGGCC 
AACAAGTTGT TTGGTGCTCC TGAGCCTTCC ACTATCGCCC GCTCTCTACC AACCACTGTC 
CCAGAGTCGC CAAACTACCG TAATGCCAGG ACTCCCCGGA CACCCCGCAC ACCGCAGCTC 
AAAGACTCAA GCCAGACATC ACGATTTTAC CCGGTGGTGA AGGAAGGACG GACAATAGAT 
GCCAAGATGC CTCGGAAAAG GAAGACAAGA CACAGTTCAA ACCCGCCATT GGAGAGTCAT 
GTGGGCTGGG TTATGGATTC TCGTGAGCAC AGGCCCCGGA CTGCTTCCAT CAGCTCCAGC 
CCCTCGGAAG GAACACCTGC AGTTGGCAGC TATGGCTGTA CCCCTCAGTC ATTGCCCAAG 
TTCCAGCATC CTTCCCATGA GCTGCTAAAG GAAAATGGCT TCACACAACA CGTCTACCAT 
AAGTATCGTA GGCGCTGCCT TAACGAGCGG AAACGCTTGG GCATTGGCCA ATCTCAGGAG 
ATGAACACTC TCTTCCGCTT CTGGTCCTTC TTCCTCCGAG ATCACTTCAA CAAAAAGATG 
TATGAGGAGT TCAAGCAGCT GGCTCTGGAG GATGCTAAAG AAGGCTACAG ATATGGTTTG 
GAGTGCCTTT TTCGATACTA CAGTTATGGC CTGGAAAAGA AGTTCCGCTT GGACATATTC 
AAGGATTTTC AGGAGGAAAC CGTGAAGGAC TACGAAGCTG GCCAACTCTA TGGGCTGGAG 
AAGTTCTGGG CCTTCCTGAA ATATTCCAAA GCCAAAAATT TGGACATTGA CCCCAAATTG 
CAAGAATACC TCGGCAAATT CCGACGTCTT GAAGACTTCC GAGTAGATCC CCCAATGGGT 
GAGGAGGGCA ACCACAAGCG ACACCCAGTG GCAGCAGGAG GTGGCGGCGG TGAGAGCAGA 
AAGAGGTGCC CTTCCCAGTC TTCCAGCAGG CCCACCACCA TGAACAGCCA ACCCCTCACG 
CCCCCCTCCG GCCAGTCCAT CCGGGAAGAT GCCAAATGGA CAAGCCAGCA CTCGGACATA 
CAGACTTTGG GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101350025 ( XM_012560139.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012415593.1 Trichechus manatus latirostris La ribonucleoprotein domain family member 1 (LOC101350025), mRNA.

Price & Availability↑

CloneID	OTr167469
Clone ID Related Accession (Same CDS sequence)	XM_012560139.1 , XM_012560139.2
Accession Version	XM_012560139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	la-related protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443947.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_012560139.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTC AGGTGGAGCC GCTGCTGCCG GGGGGCGCCC CGCTCTTGCA GGCCGAAGAG 
CACGGGGGGC TGGTGAGGAA GAAGCTGCCG CCGCCGCCCG AGGGCAAGGG CGAGCCTGGG 
GCCAACGACG TCGGAGTCGG GGAGCCGGAC GGCGGCACTC GGAGACCCCG GCTGCCCTGC 
GCCAAGCCGT ACAAGGAGGG CACCGGGCAA CAGGAGCGCG AGAGTCCGCG GCCGCCGCAG 
CCGCCGCAGC CGCCAGGCGC CGAAGGCCCG GCCGTCAGCG ACGGGGAGGA GGGCGGCGGC 
GGCGAGCCGG GCGCTGGCGG AGGAGCAACC GGAGCTGCGG GGGGCGGCGG GCGGCGAGAC 
TTCGTGGAGG CCCCCCCGCC CAAGGTGAAC CCGTGGACTA AGAACGCGTT GCCGCAGGCC 
CTGGCCACGG TGAACGGACA GTCCCCTCCA GAACCTTCTG CCCCAACCAA GGTTGTGAGG 
GCAGCAATTC CTAAACAGCG CAAAGGCAGC AAGGTTGGTG ACTTTGGAGA TGCAATCAAC 
TGGCCCACAC CAGGAGAAAT AGCCCACAAG AGTGTACAGC CACAGCCCCA CAAGTCTCAG 
CCTGCCCGTA AGCCACCACC CAAGAAGGAC ATGAGGGAAC AGGAGAAAGG AGAGGGGAGT 
GATAGTAAGG AGAGCCCAAA AACCAAGTCA GATGAATCAG GGGAGGAGAA GAATGGGGAC 
GAAGACTGTC AACGAGGAGG GCAGAAGAAG AAAGGGAACA AACACAAATG GGTTCCATTG 
CAAATAGACA TGAAGCCTGA AGCACCCAGA GAGAAACTGA CCTCGCGCCC CTCTCGCCCA 
CCAGATCCTC GTCACACACC TGCCAACCGC GGGGAGATCA AAGGGTCTGA GCCTGCTACC 
TACGTGCCCG TGCCCGTGGC CCCCCCCACC CCAGCCTGGC AACCAGAGAC CAAACCGGAG 
CCTGCCTGGC ATGACCAGGA TGAGACATCG AGTGTGAAGA GTGATGGGGC TGGTGGGGCG 
CGGGCTTCCT TCCGTGGCCG TGGACGGGGG CGTGGTCGCG GCCGGGGACG CGGCCGGGGT 
GGCACTCGAA CCCATTTCGA CTACCAGTTT GGCTACCGAA AGTTTGATGC TGCAGAGGGG 
CCTCGCATCC CCAAGTATAT GAACAACATC ACCTACTATT TTGACAATGT GAGCAGCACT 
GAGCTTTACA GCGTGGACCA GGAGCTGCTC AAAGACTACA TCAAGCGCCA GATTGAATAC 
TACTTCAGCG TGGACAATTT AGAGCGAGAC TTCTTCCTGC GACGAAAAAT GGATGCCAGC 
GGTTTCCTTC CCATCACCCT TATTGCTTCC TTCCACCGAG TGCAGGCCCT TACCACTGAC 
ATTTCACTCA TCTTTGCGGC CCTCAAGGAC AGCAAGGTGG TGGAGATAGT TGATGAGAAG 
GTCCGCAGGA GGGAGGAGCC TGAGAAGTGG CCTCTTCCTG GTCCCCCAAT GGTGGACTAT 
TCACAAACCG ATTTCTCCCA GCTTCTCAAC TGCCCCGAGT TTGTTCCTCG CCAGCACTAT 
CAGAAGGAGA CAGAATCGGC CCCTGGCTCT CCTCGGGCAG TCACTCCAGT GCCAACCAAA 
ACCGAGGAGG TCAGCAACCT AAAGACGCTG CCCAAGGGCC TGTCTGCCAG TCTGCCTGAC 
CTGGATTCTG AGAACTGGAT TGAAGTAAAG AAGAGGCCTC GTCCCTCCCC AGCACGGCCC 
AAGAAGTCAG AGGAGCCCAG GTTCCTCCAC CAGACCTCTC TGCCTCAGCA GCTGCCCTCT 
CAGCAGCTGA TGTCCAAGGA TCAGGATGAG CAAGAGGAAC TAGATTTTCT GTTTGATGAG 
GAGATGGAGC AGATGGATGG ACGGAAGAAC ACCTTTACCG CCTGGTCTGA TGAGGACTCT 
GACTATGAGA TTGACGACCG GGACGTCAAC AAGATCCTCA TTGTCACCCA GACGCCACCT 
TACATGCGCC GGCACCCAGG GGGGGACCGC ACAGGCAACC ACACCTCACG TGCCAAGATG 
AGCGCTGAGC TGGCCAAGGT CATTAACGAT GGGCTCTTCT ACTATGAGCA GGACCTGTGG 
ACTGAGAAGT TTGAACCTGA GTATTCCCAG ATCAAGCAAG AAGTTGAGAA CTTCAAGAAA 
GTCAACATGA TCAGCCGGGA GCAGTTTGAC ACTCTGACCC CCGAACCCCC TGTGGATCCC 
AACCAGGAAG TCCCTCCTGG GCCACCCCGG TTCCAGCAAG TTCCCACTGA TGCCCTGGCC 
AACAAGTTGT TTGGTGCTCC TGAGCCTTCC ACTATCGCCC GCTCTCTACC AACCACTGTC 
CCAGAGTCGC CAAACTACCG TAATGCCAGG ACTCCCCGGA CACCCCGCAC ACCGCAGCTC 
AAAGACTCAA GCCAGACATC ACGATTTTAC CCGGTGGTGA AGGAAGGACG GACAATAGAT 
GCCAAGATGC CTCGGAAAAG GAAGACAAGA CACAGTTCAA ACCCGCCATT GGAGAGTCAT 
GTGGGCTGGG TTATGGATTC TCGTGAGCAC AGGCCCCGGA CTGCTTCCAT CAGCTCCAGC 
CCCTCGGAAG GAACACCTGC AGTTGGCAGC TATGGCTGTA CCCCTCAGTC ATTGCCCAAG 
TTCCAGCATC CTTCCCATGA GCTGCTAAAG GAAAATGGCT TCACACAACA CGTCTACCAT 
AAGTATCGTA GGCGCTGCCT TAACGAGCGG AAACGCTTGG GCATTGGCCA ATCTCAGGAG 
ATGAACACTC TCTTCCGCTT CTGGTCCTTC TTCCTCCGAG ATCACTTCAA CAAAAAGATG 
TATGAGGAGT TCAAGCAGCT GGCTCTGGAG GATGCTAAAG AAGGCTACAG ATATGGTTTG 
GAGTGCCTTT TTCGATACTA CAGTTATGGC CTGGAAAAGA AGTTCCGCTT GGACATATTC 
AAGGATTTTC AGGAGGAAAC CGTGAAGGAC TACGAAGCTG GCCAACTCTA TGGGCTGGAG 
AAGTTCTGGG CCTTCCTGAA ATATTCCAAA GCCAAAAATT TGGACATTGA CCCCAAATTG 
CAAGAATACC TCGGCAAATT CCGACGTCTT GAAGACTTCC GAGTAGATCC CCCAATGGGT 
GAGGAGGGCA ACCACAAGCG ACACCCAGTG GCAGCAGGAG GTGGCGGCGG TGAGAGCAGA 
AAGAGGTGCC CTTCCCAGTC TTCCAGCAGG CCCACCACCA TGAACAGCCA ACCCCTCACG 
CCCCCCTCCG GCCAGTCCAT CCGGGAAGAT GCCAAATGGA CAAGCCAGCA CTCGGACATA 
CAGACTTTGG GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012415593.1
CDS	176..3493
Translation	MATQVEPLLPGGAPLLQAEEHGGLVRKKLPPPPEGKGEPGANDVGVGEPDGGTRRPRLPCAKPYKEGTGQQERESPRPPQPPQPPGAEGPAVSDGEEGGGGEPGAGGGATGAAGGGGRRDFVEAPPPKVNPWTKNALPQALATVNGQSPPEPSAPTKVVRAAIPKQRKGSKVGDFGDAINWPTPGEIAHKSVQPQPHKSQPARKPPPKKDMREQEKGEGSDSKESPKTKSDESGEEKNGDEDCQRGGQKKKGNKHKWVPLQIDMKPEAPREKLTSRPSRPPDPRHTPANRGEIKGSEPATYVPVPVAPPTPAWQPETKPEPAWHDQDETSSVKSDGAGGARASFRGRGRGRGRGRGRGRGGTRTHFDYQFGYRKFDAAEGPRIPKYMNNITYYFDNVSSTELYSVDQELLKDYIKRQIEYYFSVDNLERDFFLRRKMDASGFLPITLIASFHRVQALTTDISLIFAALKDSKVVEIVDEKVRRREEPEKWPLPGPPMVDYSQTDFSQLLNCPEFVPRQHYQKETESAPGSPRAVTPVPTKTEEVSNLKTLPKGLSASLPDLDSENWIEVKKRPRPSPARPKKSEEPRFLHQTSLPQQLPSQQLMSKDQDEQEELDFLFDEEMEQMDGRKNTFTAWSDEDSDYEIDDRDVNKILIVTQTPPYMRRHPGGDRTGNHTSRAKMSAELAKVINDGLFYYEQDLWTEKFEPEYSQIKQEVENFKKVNMISREQFDTLTPEPPVDPNQEVPPGPPRFQQVPTDALANKLFGAPEPSTIARSLPTTVPESPNYRNARTPRTPRTPQLKDSSQTSRFYPVVKEGRTIDAKMPRKRKTRHSSNPPLESHVGWVMDSREHRPRTASISSSPSEGTPAVGSYGCTPQSLPKFQHPSHELLKENGFTQHVYHKYRRRCLNERKRLGIGQSQEMNTLFRFWSFFLRDHFNKKMYEEFKQLALEDAKEGYRYGLECLFRYYSYGLEKKFRLDIFKDFQEETVKDYEAGQLYGLEKFWAFLKYSKAKNLDIDPKLQEYLGKFRRLEDFRVDPPMGEEGNHKRHPVAAGGGGGESRKRCPSQSSSRPTTMNSQPLTPPSGQSIREDAKWTSQHSDIQTLGK

Target ORF information:

RefSeq Version	XM_012560139.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris La ribonucleoprotein domain family member 1 (LOC101350025), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012560139.2

ORF Insert Sequence:

ATGGCCACTC AGGTGGAGCC GCTGCTGCCG GGGGGCGCCC CGCTCTTGCA GGCCGAAGAG 
CACGGGGGGC TGGTGAGGAA GAAGCTGCCG CCGCCGCCCG AGGGCAAGGG CGAGCCTGGG 
GCCAACGACG TCGGAGTCGG GGAGCCGGAC GGCGGCACTC GGAGACCCCG GCTGCCCTGC 
GCCAAGCCGT ACAAGGAGGG CACCGGGCAA CAGGAGCGCG AGAGTCCGCG GCCGCCGCAG 
CCGCCGCAGC CGCCAGGCGC CGAAGGCCCG GCCGTCAGCG ACGGGGAGGA GGGCGGCGGC 
GGCGAGCCGG GCGCTGGCGG AGGAGCAACC GGAGCTGCGG GGGGCGGCGG GCGGCGAGAC 
TTCGTGGAGG CCCCCCCGCC CAAGGTGAAC CCGTGGACTA AGAACGCGTT GCCGCAGGCC 
CTGGCCACGG TGAACGGACA GTCCCCTCCA GAACCTTCTG CCCCAACCAA GGTTGTGAGG 
GCAGCAATTC CTAAACAGCG CAAAGGCAGC AAGGTTGGTG ACTTTGGAGA TGCAATCAAC 
TGGCCCACAC CAGGAGAAAT AGCCCACAAG AGTGTACAGC CACAGCCCCA CAAGTCTCAG 
CCTGCCCGTA AGCCACCACC CAAGAAGGAC ATGAGGGAAC AGGAGAAAGG AGAGGGGAGT 
GATAGTAAGG AGAGCCCAAA AACCAAGTCA GATGAATCAG GGGAGGAGAA GAATGGGGAC 
GAAGACTGTC AACGAGGAGG GCAGAAGAAG AAAGGGAACA AACACAAATG GGTTCCATTG 
CAAATAGACA TGAAGCCTGA AGCACCCAGA GAGAAACTGA CCTCGCGCCC CTCTCGCCCA 
CCAGATCCTC GTCACACACC TGCCAACCGC GGGGAGATCA AAGGGTCTGA GCCTGCTACC 
TACGTGCCCG TGCCCGTGGC CCCCCCCACC CCAGCCTGGC AACCAGAGAC CAAACCGGAG 
CCTGCCTGGC ATGACCAGGA TGAGACATCG AGTGTGAAGA GTGATGGGGC TGGTGGGGCG 
CGGGCTTCCT TCCGTGGCCG TGGACGGGGG CGTGGTCGCG GCCGGGGACG CGGCCGGGGT 
GGCACTCGAA CCCATTTCGA CTACCAGTTT GGCTACCGAA AGTTTGATGC TGCAGAGGGG 
CCTCGCATCC CCAAGTATAT GAACAACATC ACCTACTATT TTGACAATGT GAGCAGCACT 
GAGCTTTACA GCGTGGACCA GGAGCTGCTC AAAGACTACA TCAAGCGCCA GATTGAATAC 
TACTTCAGCG TGGACAATTT AGAGCGAGAC TTCTTCCTGC GACGAAAAAT GGATGCCAGC 
GGTTTCCTTC CCATCACCCT TATTGCTTCC TTCCACCGAG TGCAGGCCCT TACCACTGAC 
ATTTCACTCA TCTTTGCGGC CCTCAAGGAC AGCAAGGTGG TGGAGATAGT TGATGAGAAG 
GTCCGCAGGA GGGAGGAGCC TGAGAAGTGG CCTCTTCCTG GTCCCCCAAT GGTGGACTAT 
TCACAAACCG ATTTCTCCCA GCTTCTCAAC TGCCCCGAGT TTGTTCCTCG CCAGCACTAT 
CAGAAGGAGA CAGAATCGGC CCCTGGCTCT CCTCGGGCAG TCACTCCAGT GCCAACCAAA 
ACCGAGGAGG TCAGCAACCT AAAGACGCTG CCCAAGGGCC TGTCTGCCAG TCTGCCTGAC 
CTGGATTCTG AGAACTGGAT TGAAGTAAAG AAGAGGCCTC GTCCCTCCCC AGCACGGCCC 
AAGAAGTCAG AGGAGCCCAG GTTCCTCCAC CAGACCTCTC TGCCTCAGCA GCTGCCCTCT 
CAGCAGCTGA TGTCCAAGGA TCAGGATGAG CAAGAGGAAC TAGATTTTCT GTTTGATGAG 
GAGATGGAGC AGATGGATGG ACGGAAGAAC ACCTTTACCG CCTGGTCTGA TGAGGACTCT 
GACTATGAGA TTGACGACCG GGACGTCAAC AAGATCCTCA TTGTCACCCA GACGCCACCT 
TACATGCGCC GGCACCCAGG GGGGGACCGC ACAGGCAACC ACACCTCACG TGCCAAGATG 
AGCGCTGAGC TGGCCAAGGT CATTAACGAT GGGCTCTTCT ACTATGAGCA GGACCTGTGG 
ACTGAGAAGT TTGAACCTGA GTATTCCCAG ATCAAGCAAG AAGTTGAGAA CTTCAAGAAA 
GTCAACATGA TCAGCCGGGA GCAGTTTGAC ACTCTGACCC CCGAACCCCC TGTGGATCCC 
AACCAGGAAG TCCCTCCTGG GCCACCCCGG TTCCAGCAAG TTCCCACTGA TGCCCTGGCC 
AACAAGTTGT TTGGTGCTCC TGAGCCTTCC ACTATCGCCC GCTCTCTACC AACCACTGTC 
CCAGAGTCGC CAAACTACCG TAATGCCAGG ACTCCCCGGA CACCCCGCAC ACCGCAGCTC 
AAAGACTCAA GCCAGACATC ACGATTTTAC CCGGTGGTGA AGGAAGGACG GACAATAGAT 
GCCAAGATGC CTCGGAAAAG GAAGACAAGA CACAGTTCAA ACCCGCCATT GGAGAGTCAT 
GTGGGCTGGG TTATGGATTC TCGTGAGCAC AGGCCCCGGA CTGCTTCCAT CAGCTCCAGC 
CCCTCGGAAG GAACACCTGC AGTTGGCAGC TATGGCTGTA CCCCTCAGTC ATTGCCCAAG 
TTCCAGCATC CTTCCCATGA GCTGCTAAAG GAAAATGGCT TCACACAACA CGTCTACCAT 
AAGTATCGTA GGCGCTGCCT TAACGAGCGG AAACGCTTGG GCATTGGCCA ATCTCAGGAG 
ATGAACACTC TCTTCCGCTT CTGGTCCTTC TTCCTCCGAG ATCACTTCAA CAAAAAGATG 
TATGAGGAGT TCAAGCAGCT GGCTCTGGAG GATGCTAAAG AAGGCTACAG ATATGGTTTG 
GAGTGCCTTT TTCGATACTA CAGTTATGGC CTGGAAAAGA AGTTCCGCTT GGACATATTC 
AAGGATTTTC AGGAGGAAAC CGTGAAGGAC TACGAAGCTG GCCAACTCTA TGGGCTGGAG 
AAGTTCTGGG CCTTCCTGAA ATATTCCAAA GCCAAAAATT TGGACATTGA CCCCAAATTG 
CAAGAATACC TCGGCAAATT CCGACGTCTT GAAGACTTCC GAGTAGATCC CCCAATGGGT 
GAGGAGGGCA ACCACAAGCG ACACCCAGTG GCAGCAGGAG GTGGCGGCGG TGAGAGCAGA 
AAGAGGTGCC CTTCCCAGTC TTCCAGCAGG CCCACCACCA TGAACAGCCA ACCCCTCACG 
CCCCCCTCCG GCCAGTCCAT CCGGGAAGAT GCCAAATGGA CAAGCCAGCA CTCGGACATA 
CAGACTTTGG GAAAGTGA