LOC101348679 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101348679
Entrez Gene ID	101348679
Full Name	NCK associated protein 1
Synonyms	NCKAP1
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004375444.2	XP_004375501.1	nck-associated protein 1 isoform X1
XM_004375445.2	XP_004375502.1	nck-associated protein 1

Pathways from BioSystems
Gene Ontology

The following LOC101348679 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101348679 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTr172197	XM_004375444.2 Latest version!	Trichechus manatus latirostris NCK-associated protein 1 (LOC101348679), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OTr172198	XM_004375445.2 Latest version!	Trichechus manatus latirostris NCK associated protein 1 (LOC101348679), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101348679 ( XM_004375444.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004375501.1 Trichechus manatus latirostris NCK-associated protein 1 (LOC101348679), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTr172197
Clone ID Related Accession (Same CDS sequence)	XM_004375444.2
Accession Version	XM_004375444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	nck-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443973.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2015 this sequence version replaced XM_004375444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GCGGAGACCC CAAGGCAAAA CCATCCTATC TTATTGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT TAGAAAATTC CCTGCTGTAG AAACCCGTAA CAACAATCAA 
CAGCTTGCCC AACTACAGAA AGAAAAATCA GAAATTCTGA AAAATCTGGC ATTATATTAC 
TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTACT GAATACGATT 
GATGTTTGCC AGGTCTTCTT TGATATCACT GTAAACTTTG ATTTAACAAA GAACTACTTA 
GATTTGATCA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG 
GCAATCATTG GATTATACAA CTATGCTCAT GAAATGACCC ATGGAGCAAG TGACAGAGAA 
TACCCACGCC TTGGCCAAAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA GATGGTATAT 
CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG TCTCATCAGT 
GCACCCAGTA CAATGCTTAA TCCTGCACAA TCTGACACTA TGCCTTGTGA ATATCTCTCT 
TTGGATGCAA TGGAAAAATG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT 
ACTGATGCTA CAGCACTGAA TCTTTGGAAG CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA 
CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC TGTGTCGCAT 
GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGGT CAGCCCTGAA AGAATTGGCT 
ACTGTCCTCT CTGATCAGCC TGGCTTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA 
TTGTCCTTTG CCCGCGATGA AATTATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG 
AAGAGTGCAG ATGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAG 
CTTAGAGCAC ATGTAAGGAA ATATGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTTGTGCAGA ATCTTTCTGT TTGCCCTGAA 
GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTCAG TGTAAAACAA 
GTTGAAGACG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGACTGGTT TAGGTTACAG 
GCATATACTA GTGTCTCTAA GGCTTCCCTT AGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG 
GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAG 
CAGTGTTTGG AGCTACCCTC TCAGTCAAGA TACTCGATTG CATTTCCACT ACTTTGCACT 
CATTTTATGA GTTGCACACA TGAACTGTGT CCAGAAGAGC GACATCATAT TGGAGATCGC 
AGTCTTTCGT TATGTAATAT GTTCCTGGAT GAAATGGCTA AGCAAGCTCG AAATCTCATC 
ACTGATATTT GCACAGAACA GTGTACTCTT AGTGACCAGT TGCTGCCCAA ACATTGTGCC 
AAAACCATCA GTCAAGCAGT GAATAAGAAG TCAAAAAAGC AGACTGGTAA GAAAGGGGAA 
CCTGAAAGGG AAAAACCAGG AGTTGAAAGC ATGAGGAAAA ACAGGCTGGT AGTGACCAAC 
CTTGATAAAC TGCACACTGC ACTTTCTGAG TTATGTTTCT CTATAAATTA TGTACCAAAC 
ATGGTGGTTT GGGAGCATAC ATTTACCCCA AGAGAATATT TGACTTCTCA TCTGGAAATC 
CGCTTTACTA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA 
AAGCCATCAG AGCTTTTAAC AAGCGTAAGA GCGTACATGA CCGTACTCCA GTCGATAGAA 
AACTATGTGC AAATTGATAT TACAAGAGTA TTTAATAATG TTCTTCTTCA ACAAACACAA 
CATTTAGATA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA 
ACCTTGCTAA GACAAGTCAG CAATGGCCAT ATAGCTTATT TCCCTGCGAT GAAAGCATTT 
GTGAACTTAC CTACAGAAAA TGAGTTAACA TTCAATGCTG AGGAATATTC TGACATTTCA 
GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC 
CTTATGTGGC ATATTTCATC CCAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTGCTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTGTTT 
AAAAGATTAT CATCCGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA 
TCCTTCCGAT CATTGGCACA AGAAGCACTG AGAGATGTTT TATCCTACCA CATTCCTTTT 
CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GAGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGTT ATCGTCAGCT GCTGGATTAC CTTGTGAGAT TGATCCTGCC 
TTGGTAGTAG CTCTCTCCTC ACAAAAATCA GAAAACATTA GCCCAGAAGA AGAATATAAA 
ATTGCCTGCC TCCTCATGGT CTTTGTGGCA GTTTCTTTGC CAACACTGGC TAGTAATGTG 
ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CACTGCAACA ATATACATTG CTTGGCCAAA 
GCCATCAACC AGATCGCAGC AGCTTTGTTT ACAATTCACA AAGGGAGCAT TGAAGACCGT 
CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTTTTAA AAATTGGCCA GGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTACTGC TCGATATGAT TGTACAGGAA 
TCCCCATTCC TAACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACAGCCTCTG GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004375501.1
CDS	459..3863
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTASG

Target ORF information:

RefSeq Version	XM_004375444.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris NCK-associated protein 1 (LOC101348679), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004375444.2

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GCGGAGACCC CAAGGCAAAA CCATCCTATC TTATTGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT TAGAAAATTC CCTGCTGTAG AAACCCGTAA CAACAATCAA 
CAGCTTGCCC AACTACAGAA AGAAAAATCA GAAATTCTGA AAAATCTGGC ATTATATTAC 
TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTACT GAATACGATT 
GATGTTTGCC AGGTCTTCTT TGATATCACT GTAAACTTTG ATTTAACAAA GAACTACTTA 
GATTTGATCA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG 
GCAATCATTG GATTATACAA CTATGCTCAT GAAATGACCC ATGGAGCAAG TGACAGAGAA 
TACCCACGCC TTGGCCAAAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA GATGGTATAT 
CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG TCTCATCAGT 
GCACCCAGTA CAATGCTTAA TCCTGCACAA TCTGACACTA TGCCTTGTGA ATATCTCTCT 
TTGGATGCAA TGGAAAAATG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT 
ACTGATGCTA CAGCACTGAA TCTTTGGAAG CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA 
CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC TGTGTCGCAT 
GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGGT CAGCCCTGAA AGAATTGGCT 
ACTGTCCTCT CTGATCAGCC TGGCTTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA 
TTGTCCTTTG CCCGCGATGA AATTATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG 
AAGAGTGCAG ATGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAG 
CTTAGAGCAC ATGTAAGGAA ATATGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTTGTGCAGA ATCTTTCTGT TTGCCCTGAA 
GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTCAG TGTAAAACAA 
GTTGAAGACG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGACTGGTT TAGGTTACAG 
GCATATACTA GTGTCTCTAA GGCTTCCCTT AGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG 
GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAG 
CAGTGTTTGG AGCTACCCTC TCAGTCAAGA TACTCGATTG CATTTCCACT ACTTTGCACT 
CATTTTATGA GTTGCACACA TGAACTGTGT CCAGAAGAGC GACATCATAT TGGAGATCGC 
AGTCTTTCGT TATGTAATAT GTTCCTGGAT GAAATGGCTA AGCAAGCTCG AAATCTCATC 
ACTGATATTT GCACAGAACA GTGTACTCTT AGTGACCAGT TGCTGCCCAA ACATTGTGCC 
AAAACCATCA GTCAAGCAGT GAATAAGAAG TCAAAAAAGC AGACTGGTAA GAAAGGGGAA 
CCTGAAAGGG AAAAACCAGG AGTTGAAAGC ATGAGGAAAA ACAGGCTGGT AGTGACCAAC 
CTTGATAAAC TGCACACTGC ACTTTCTGAG TTATGTTTCT CTATAAATTA TGTACCAAAC 
ATGGTGGTTT GGGAGCATAC ATTTACCCCA AGAGAATATT TGACTTCTCA TCTGGAAATC 
CGCTTTACTA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA 
AAGCCATCAG AGCTTTTAAC AAGCGTAAGA GCGTACATGA CCGTACTCCA GTCGATAGAA 
AACTATGTGC AAATTGATAT TACAAGAGTA TTTAATAATG TTCTTCTTCA ACAAACACAA 
CATTTAGATA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA 
ACCTTGCTAA GACAAGTCAG CAATGGCCAT ATAGCTTATT TCCCTGCGAT GAAAGCATTT 
GTGAACTTAC CTACAGAAAA TGAGTTAACA TTCAATGCTG AGGAATATTC TGACATTTCA 
GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC 
CTTATGTGGC ATATTTCATC CCAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTGCTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTGTTT 
AAAAGATTAT CATCCGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA 
TCCTTCCGAT CATTGGCACA AGAAGCACTG AGAGATGTTT TATCCTACCA CATTCCTTTT 
CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GAGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGTT ATCGTCAGCT GCTGGATTAC CTTGTGAGAT TGATCCTGCC 
TTGGTAGTAG CTCTCTCCTC ACAAAAATCA GAAAACATTA GCCCAGAAGA AGAATATAAA 
ATTGCCTGCC TCCTCATGGT CTTTGTGGCA GTTTCTTTGC CAACACTGGC TAGTAATGTG 
ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CACTGCAACA ATATACATTG CTTGGCCAAA 
GCCATCAACC AGATCGCAGC AGCTTTGTTT ACAATTCACA AAGGGAGCAT TGAAGACCGT 
CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTTTTAA AAATTGGCCA GGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTACTGC TCGATATGAT TGTACAGGAA 
TCCCCATTCC TAACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACAGCCTCTG GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101348679 ( XM_004375445.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004375502.1 Trichechus manatus latirostris NCK associated protein 1 (LOC101348679), mRNA.

Price & Availability↑

CloneID	OTr172198
Clone ID Related Accession (Same CDS sequence)	XM_004375445.2
Accession Version	XM_004375445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	nck-associated protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443973.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2015 this sequence version replaced XM_004375445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGCGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATTGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTTAGAAAAT TCCCTGCTGT AGAAACCCGT AACAACAATC AACAGCTTGC CCAACTACAG 
AAAGAAAAAT CAGAAATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT TTGTGAATTA CTGAATACGA TTGATGTTTG CCAGGTCTTC 
TTTGATATCA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTGAT CATAACCTAT 
ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC 
AACTATGCTC ATGAAATGAC CCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAA 
ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCACT AATTTCTCTT CAGATGGTAT ATCCTCGAAG GAATCTTTCA 
GCTGACCAGT GGAGAAATGC CCAGTTATTG AGTCTCATCA GTGCACCCAG TACAATGCTT 
AATCCTGCAC AATCTGACAC TATGCCTTGT GAATATCTCT CTTTGGATGC AATGGAAAAA 
TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGATGC TACAGCACTG 
AATCTTTGGA AGCTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT 
ATTAATGACA TAAGAGAATG CAAGGAGGCA GCTGTGTCGC ATGCTGGTTC AATGCACAGA 
GAAAGACGCA AGTTTTTAAG GTCAGCCCTG AAAGAATTGG CTACTGTCCT CTCTGATCAG 
CCTGGCTTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTGTCCTT TGCCCGCGAT 
GAAATTATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGATGACTTT 
ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AGCTTAGAGC ACATGTAAGG 
AAATATGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTTGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG 
TCCTCTTTTG TTAACACTAT GACTTCCCTC AGTGTAAAAC AAGTTGAAGA CGGGGAAGTA 
TTTGATTTCA GAGGAATGAG ATTAGACTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCT 
AAGGCTTCCC TTAGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT 
TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC 
ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTACCC 
TCTCAGTCAA GATACTCGAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACA 
CATGAACTGT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC GTTATGTAAT 
ATGTTCCTGG ATGAAATGGC TAAGCAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA 
CAGTGTACTC TTAGTGACCA GTTGCTGCCC AAACATTGTG CCAAAACCAT CAGTCAAGCA 
GTGAATAAGA AGTCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAAAAACCA 
GGAGTTGAAA GCATGAGGAA AAACAGGCTG GTAGTGACCA ACCTTGATAA ACTGCACACT 
GCACTTTCTG AGTTATGTTT CTCTATAAAT TATGTACCAA ACATGGTGGT TTGGGAGCAT 
ACATTTACCC CAAGAGAATA TTTGACTTCT CATCTGGAAA TCCGCTTTAC TAAGTCAATT 
GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAGCCATC AGAGCTTTTA 
ACAAGCGTAA GAGCGTACAT GACCGTACTC CAGTCGATAG AAAACTATGT GCAAATTGAT 
ATTACAAGAG TATTTAATAA TGTTCTTCTT CAACAAACAC AACATTTAGA TAGTCATGGA 
GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACCTTGCT AAGACAAGTC 
AGCAATGGCC ATATAGCTTA TTTCCCTGCG ATGAAAGCAT TTGTGAACTT ACCTACAGAA 
AATGAGTTAA CATTCAATGC TGAGGAATAT TCTGACATTT CAGAAATGAG GTCATTATCA 
GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCA 
TCCCAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGCT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTGT TTAAAAGATT ATCATCCGTT 
GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA 
CAAGAAGCAC TGAGAGATGT TTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA 
GATTTTAAGG ATCACATTCC AAGAGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
TTATCGTCAG CTGCTGGATT ACCTTGTGAG ATTGATCCTG CCTTGGTAGT AGCTCTCTCC 
TCACAAAAAT CAGAAAACAT TAGCCCAGAA GAAGAATATA AAATTGCCTG CCTCCTCATG 
GTCTTTGTGG CAGTTTCTTT GCCAACACTG GCTAGTAATG TGATGTCTCA GTACAGCCCT 
GCTATAGAAG GGCACTGCAA CAATATACAT TGCTTGGCCA AAGCCATCAA CCAGATCGCA 
GCAGCTTTGT TTACAATTCA CAAAGGGAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG 
CTTGCATCCT CCAGTCTTTT AAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCTG TTTATTTACT GCTCGATATG ATTGTACAGG AATCCCCATT CCTAACAATG 
GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACAGCCTC TGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004375502.1
CDS	459..3845
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTASG

Target ORF information:

RefSeq Version	XM_004375445.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris NCK associated protein 1 (LOC101348679), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004375445.2

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGCGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATTGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTTAGAAAAT TCCCTGCTGT AGAAACCCGT AACAACAATC AACAGCTTGC CCAACTACAG 
AAAGAAAAAT CAGAAATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT TTGTGAATTA CTGAATACGA TTGATGTTTG CCAGGTCTTC 
TTTGATATCA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTGAT CATAACCTAT 
ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC 
AACTATGCTC ATGAAATGAC CCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAA 
ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCACT AATTTCTCTT CAGATGGTAT ATCCTCGAAG GAATCTTTCA 
GCTGACCAGT GGAGAAATGC CCAGTTATTG AGTCTCATCA GTGCACCCAG TACAATGCTT 
AATCCTGCAC AATCTGACAC TATGCCTTGT GAATATCTCT CTTTGGATGC AATGGAAAAA 
TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGATGC TACAGCACTG 
AATCTTTGGA AGCTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT 
ATTAATGACA TAAGAGAATG CAAGGAGGCA GCTGTGTCGC ATGCTGGTTC AATGCACAGA 
GAAAGACGCA AGTTTTTAAG GTCAGCCCTG AAAGAATTGG CTACTGTCCT CTCTGATCAG 
CCTGGCTTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTGTCCTT TGCCCGCGAT 
GAAATTATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGATGACTTT 
ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AGCTTAGAGC ACATGTAAGG 
AAATATGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTTGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG 
TCCTCTTTTG TTAACACTAT GACTTCCCTC AGTGTAAAAC AAGTTGAAGA CGGGGAAGTA 
TTTGATTTCA GAGGAATGAG ATTAGACTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCT 
AAGGCTTCCC TTAGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT 
TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC 
ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTACCC 
TCTCAGTCAA GATACTCGAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACA 
CATGAACTGT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC GTTATGTAAT 
ATGTTCCTGG ATGAAATGGC TAAGCAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA 
CAGTGTACTC TTAGTGACCA GTTGCTGCCC AAACATTGTG CCAAAACCAT CAGTCAAGCA 
GTGAATAAGA AGTCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAAAAACCA 
GGAGTTGAAA GCATGAGGAA AAACAGGCTG GTAGTGACCA ACCTTGATAA ACTGCACACT 
GCACTTTCTG AGTTATGTTT CTCTATAAAT TATGTACCAA ACATGGTGGT TTGGGAGCAT 
ACATTTACCC CAAGAGAATA TTTGACTTCT CATCTGGAAA TCCGCTTTAC TAAGTCAATT 
GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAGCCATC AGAGCTTTTA 
ACAAGCGTAA GAGCGTACAT GACCGTACTC CAGTCGATAG AAAACTATGT GCAAATTGAT 
ATTACAAGAG TATTTAATAA TGTTCTTCTT CAACAAACAC AACATTTAGA TAGTCATGGA 
GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACCTTGCT AAGACAAGTC 
AGCAATGGCC ATATAGCTTA TTTCCCTGCG ATGAAAGCAT TTGTGAACTT ACCTACAGAA 
AATGAGTTAA CATTCAATGC TGAGGAATAT TCTGACATTT CAGAAATGAG GTCATTATCA 
GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCA 
TCCCAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGCT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTGT TTAAAAGATT ATCATCCGTT 
GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA 
CAAGAAGCAC TGAGAGATGT TTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA 
GATTTTAAGG ATCACATTCC AAGAGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
TTATCGTCAG CTGCTGGATT ACCTTGTGAG ATTGATCCTG CCTTGGTAGT AGCTCTCTCC 
TCACAAAAAT CAGAAAACAT TAGCCCAGAA GAAGAATATA AAATTGCCTG CCTCCTCATG 
GTCTTTGTGG CAGTTTCTTT GCCAACACTG GCTAGTAATG TGATGTCTCA GTACAGCCCT 
GCTATAGAAG GGCACTGCAA CAATATACAT TGCTTGGCCA AAGCCATCAA CCAGATCGCA 
GCAGCTTTGT TTACAATTCA CAAAGGGAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG 
CTTGCATCCT CCAGTCTTTT AAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCTG TTTATTTACT GCTCGATATG ATTGTACAGG AATCCCCATT CCTAACAATG 
GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACAGCCTC TGGGTAA