LOC101344460 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101344460
Entrez Gene ID	101344460
Full Name	phospholipase C delta 3
Synonyms	PLCD3
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004374276.2	XP_004374333.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3
XM_004374276.1	XP_004374333.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3

Pathways from BioSystems
Gene Ontology

The following LOC101344460 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101344460 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr170874	XM_004374276.2 Latest version!	Trichechus manatus latirostris phospholipase C delta 3 (LOC101344460), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OTr170874	XM_004374276.1	Trichechus manatus latirostris phospholipase C, delta 3 (LOC101344460), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101344460 ( XM_004374276.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004374333.1 Trichechus manatus latirostris phospholipase C delta 3 (LOC101344460), mRNA.

Price & Availability↑

CloneID	OTr170874
Clone ID Related Accession (Same CDS sequence)	XM_004374276.2 , XM_004374276.1
Accession Version	XM_004374276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443964.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_004374276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCG GCCGCTGGAG GAGCTGTCGC CGCCAGCCCG AGGAGCCCCC GGTGTCGGCC 
CACGTCGCGG CGCCCGTCGC GCTGCCGCCG CAGCCCGCGC CCACGGACGG CGGCATCAAG 
AGGCCCGGGC TGCGGGCGCT GAAAAAGATG GGCCTGACGG AGGACGAGGA CGTGCGCGCC 
ATGCTGCGGG GCTCGCGGCT CCGCAAGATC CGCTCGCGGA CGTGGCAGAA GGAGCGGCTG 
TACCGGCTGC AGGAGGACTG CCTGAGCGTG TGGTTCCAGC GGCGCATCCC GCGGGCGCCG 
TCGCAGCACA TCTTCTACGT GCAACACATC GAGGCCGTCC GCGAGGGCCA CCAGTCCGAA 
GGCCTGCAGC GCTTCGGCAG CGCCTTCGCG CCCGCACGCT GCCTCACCAT CGCCTTCAAG 
GGCCGCCGCA AGAACCTGGA CCTGGTGGCG CCCACGGCGG AAGAGGCGCA GCGCTGGGTG 
CGCGGCCTGG CCAAGCTGCG CGCACGCCTC GACGCCATGA GCCAGAGGGA GCGGCTCGAC 
CATTGGATTC ACTTCTACCT GCACCGGGCA GACTCCAACC AGGATAGTAA GATGAGCTTC 
AAGGAGATCA AGAGCCTGCT CAGAATGGTC AATGTGGACA TGAATGACAT GTATGCCTAC 
CACCTCTTCA AGGAGTGCGA CCACTCCAAC AACGAGAAGC TGGAAGGGGC TGAGATAGAG 
GAATTTCTGC GGCGGCTGCT GAAACGCCCT GAGTTGGAGG AGATCTTCCA TCGGTACTCG 
GGCGAGGACC ACGTGCTGAG CGCCCCTGAA CTGCTGGAGT TCCTGGAGGA CCAGGGTGAG 
GACGATGCCA CACTGACCCA CGCCCAGCAG CTCATCCAGA CCTATGAGCT CAACGAGACA 
GCCAAACAGC ATGAGCTGAT GACACTGGAT GGCTTCATGA TGTACCTGCT GTCGCCTGAA 
GGGGCTGCCC TGGACCCGGC CCACACACGC GTGTTCCAGG ACATGGACCA ACCCCTAACC 
CACTACTTCA TCTCCTCCTC CCACAATACC TACCTGACCG ACTCTCAGAT CGGGGGGCCT 
AGCAGCACCG AGGCCTATGT TAGGGCCTTT GCCCAGGGAT GCCGCTGTGT GGAGCTAGAC 
TGCTGGGAGG GCCCAGGAGG GGAACCTGTC ATCTACCATG GCCATACACT TACCTCCAAG 
ATCCTTTTCC GCGACGTGGT CCAAGCTGTG CGTGACCATG CCTTCATGCT CTCCCCATAC 
CCTGTCATCC TGTCCCTCGA GAACCACTGT GGGCTGGAGC AGCAAGCTGC CATGGCCCGC 
CACCTCCGCA CCATCCTGGG GGACATGCTG GTGACACAGG CACTGGACTC CCAGAGCCCT 
GAGGCAGTAC CATCTCCAGA GCAACTGAAG GGCCGTGTCC TGGTGAAGGG GAAGAAGCTG 
CCGGCTGCTC GGAGCGAGGA TGGCCGAATT TTACCGGACA GGGAGGAGGA GGAGGAGGAA 
GAAGAGGAAG AGGAAAAAGA GGAGGCAGAG GGTGCAGAGC AGAAGCGGAG GGCCAAGCAG 
ATCTCCCCAG AGCTGTCAGC CCTGGCTGTG TACTGCTGCG CTACCCGCCT GCAGACCCTG 
CACCTGGCCT CTGTGCCAGC CCAGCCCTGC CAGGTCAGCT CCCTCAGCGA GCGTAAGGCT 
AAGAAGCTCA TCCGAGAGGC AGGGAACAGC TTTGTCAGGC ACAATGCTCG GCAGCTGACC 
CGCGTCTACC CGCTGGGGCT TCGGATGAAC TCGGCCAATT ACAGCCCTCA GGAGATGTGG 
AACTCAGGCT GCCAGCTGGT GGCCCTGAAT TTCCAGACGC CCGGCTATGA GATGGACCTC 
AATGCTGGGC GCTTCCTCAT CAACGGGCAG TGTGGATATG TCCTGAAACC TGACTGCCTA 
CGGCAACCCG ACACAACCTT TGACCCTGAG TGCCCTAGGC CCCCCAGGAC CACACTTACC 
ATCCAGGTGC TGACTGCACA GCAGCTGCCC AAGCTGAACG CCGAGAAGCC CAACTCCATC 
GTGGACCCCC TGGTACGCAT CGAGATCCAC GGGGTGCCGG CAGACTGTGC ACGCAAGGAG 
ACTGACTACG TGCTCAACAA TGGCTTCAAT CCACATTGGG GGCAGACCCT GCAGTTCCAG 
TTGCGGGCTC CGGAGCTGGT GCTGGTCCGG TTCGTGGTCG AGGACTATGA CACCACCTCC 
CCCAATGACT TTGTGGGCCA GTTTACACTG CCTCTGAACA GCCTGAAGCA AGGGTACCGC 
CACATCCACC TGCTTTCCAA GGATGGGGCC TCACTCTCAC CAGCCACGCT CTTCGTCCAC 
ATCCGGATCC AGCACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004374333.1
CDS	95..2455
Translation	MLCGRWRSCRRQPEEPPVSAHVAAPVALPPQPAPTDGGIKRPGLRALKKMGLTEDEDVRAMLRGSRLRKIRSRTWQKERLYRLQEDCLSVWFQRRIPRAPSQHIFYVQHIEAVREGHQSEGLQRFGSAFAPARCLTIAFKGRRKNLDLVAPTAEEAQRWVRGLAKLRARLDAMSQRERLDHWIHFYLHRADSNQDSKMSFKEIKSLLRMVNVDMNDMYAYHLFKECDHSNNEKLEGAEIEEFLRRLLKRPELEEIFHRYSGEDHVLSAPELLEFLEDQGEDDATLTHAQQLIQTYELNETAKQHELMTLDGFMMYLLSPEGAALDPAHTRVFQDMDQPLTHYFISSSHNTYLTDSQIGGPSSTEAYVRAFAQGCRCVELDCWEGPGGEPVIYHGHTLTSKILFRDVVQAVRDHAFMLSPYPVILSLENHCGLEQQAAMARHLRTILGDMLVTQALDSQSPEAVPSPEQLKGRVLVKGKKLPAARSEDGRILPDREEEEEEEEEEEKEEAEGAEQKRRAKQISPELSALAVYCCATRLQTLHLASVPAQPCQVSSLSERKAKKLIREAGNSFVRHNARQLTRVYPLGLRMNSANYSPQEMWNSGCQLVALNFQTPGYEMDLNAGRFLINGQCGYVLKPDCLRQPDTTFDPECPRPPRTTLTIQVLTAQQLPKLNAEKPNSIVDPLVRIEIHGVPADCARKETDYVLNNGFNPHWGQTLQFQLRAPELVLVRFVVEDYDTTSPNDFVGQFTLPLNSLKQGYRHIHLLSKDGASLSPATLFVHIRIQHC

Target ORF information:

RefSeq Version	XM_004374276.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris phospholipase C delta 3 (LOC101344460), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004374276.2

ORF Insert Sequence:

ATGCTGTGCG GCCGCTGGAG GAGCTGTCGC CGCCAGCCCG AGGAGCCCCC GGTGTCGGCC 
CACGTCGCGG CGCCCGTCGC GCTGCCGCCG CAGCCCGCGC CCACGGACGG CGGCATCAAG 
AGGCCCGGGC TGCGGGCGCT GAAAAAGATG GGCCTGACGG AGGACGAGGA CGTGCGCGCC 
ATGCTGCGGG GCTCGCGGCT CCGCAAGATC CGCTCGCGGA CGTGGCAGAA GGAGCGGCTG 
TACCGGCTGC AGGAGGACTG CCTGAGCGTG TGGTTCCAGC GGCGCATCCC GCGGGCGCCG 
TCGCAGCACA TCTTCTACGT GCAACACATC GAGGCCGTCC GCGAGGGCCA CCAGTCCGAA 
GGCCTGCAGC GCTTCGGCAG CGCCTTCGCG CCCGCACGCT GCCTCACCAT CGCCTTCAAG 
GGCCGCCGCA AGAACCTGGA CCTGGTGGCG CCCACGGCGG AAGAGGCGCA GCGCTGGGTG 
CGCGGCCTGG CCAAGCTGCG CGCACGCCTC GACGCCATGA GCCAGAGGGA GCGGCTCGAC 
CATTGGATTC ACTTCTACCT GCACCGGGCA GACTCCAACC AGGATAGTAA GATGAGCTTC 
AAGGAGATCA AGAGCCTGCT CAGAATGGTC AATGTGGACA TGAATGACAT GTATGCCTAC 
CACCTCTTCA AGGAGTGCGA CCACTCCAAC AACGAGAAGC TGGAAGGGGC TGAGATAGAG 
GAATTTCTGC GGCGGCTGCT GAAACGCCCT GAGTTGGAGG AGATCTTCCA TCGGTACTCG 
GGCGAGGACC ACGTGCTGAG CGCCCCTGAA CTGCTGGAGT TCCTGGAGGA CCAGGGTGAG 
GACGATGCCA CACTGACCCA CGCCCAGCAG CTCATCCAGA CCTATGAGCT CAACGAGACA 
GCCAAACAGC ATGAGCTGAT GACACTGGAT GGCTTCATGA TGTACCTGCT GTCGCCTGAA 
GGGGCTGCCC TGGACCCGGC CCACACACGC GTGTTCCAGG ACATGGACCA ACCCCTAACC 
CACTACTTCA TCTCCTCCTC CCACAATACC TACCTGACCG ACTCTCAGAT CGGGGGGCCT 
AGCAGCACCG AGGCCTATGT TAGGGCCTTT GCCCAGGGAT GCCGCTGTGT GGAGCTAGAC 
TGCTGGGAGG GCCCAGGAGG GGAACCTGTC ATCTACCATG GCCATACACT TACCTCCAAG 
ATCCTTTTCC GCGACGTGGT CCAAGCTGTG CGTGACCATG CCTTCATGCT CTCCCCATAC 
CCTGTCATCC TGTCCCTCGA GAACCACTGT GGGCTGGAGC AGCAAGCTGC CATGGCCCGC 
CACCTCCGCA CCATCCTGGG GGACATGCTG GTGACACAGG CACTGGACTC CCAGAGCCCT 
GAGGCAGTAC CATCTCCAGA GCAACTGAAG GGCCGTGTCC TGGTGAAGGG GAAGAAGCTG 
CCGGCTGCTC GGAGCGAGGA TGGCCGAATT TTACCGGACA GGGAGGAGGA GGAGGAGGAA 
GAAGAGGAAG AGGAAAAAGA GGAGGCAGAG GGTGCAGAGC AGAAGCGGAG GGCCAAGCAG 
ATCTCCCCAG AGCTGTCAGC CCTGGCTGTG TACTGCTGCG CTACCCGCCT GCAGACCCTG 
CACCTGGCCT CTGTGCCAGC CCAGCCCTGC CAGGTCAGCT CCCTCAGCGA GCGTAAGGCT 
AAGAAGCTCA TCCGAGAGGC AGGGAACAGC TTTGTCAGGC ACAATGCTCG GCAGCTGACC 
CGCGTCTACC CGCTGGGGCT TCGGATGAAC TCGGCCAATT ACAGCCCTCA GGAGATGTGG 
AACTCAGGCT GCCAGCTGGT GGCCCTGAAT TTCCAGACGC CCGGCTATGA GATGGACCTC 
AATGCTGGGC GCTTCCTCAT CAACGGGCAG TGTGGATATG TCCTGAAACC TGACTGCCTA 
CGGCAACCCG ACACAACCTT TGACCCTGAG TGCCCTAGGC CCCCCAGGAC CACACTTACC 
ATCCAGGTGC TGACTGCACA GCAGCTGCCC AAGCTGAACG CCGAGAAGCC CAACTCCATC 
GTGGACCCCC TGGTACGCAT CGAGATCCAC GGGGTGCCGG CAGACTGTGC ACGCAAGGAG 
ACTGACTACG TGCTCAACAA TGGCTTCAAT CCACATTGGG GGCAGACCCT GCAGTTCCAG 
TTGCGGGCTC CGGAGCTGGT GCTGGTCCGG TTCGTGGTCG AGGACTATGA CACCACCTCC 
CCCAATGACT TTGTGGGCCA GTTTACACTG CCTCTGAACA GCCTGAAGCA AGGGTACCGC 
CACATCCACC TGCTTTCCAA GGATGGGGCC TCACTCTCAC CAGCCACGCT CTTCGTCCAC 
ATCCGGATCC AGCACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101344460 ( XM_004374276.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004374333.1 Trichechus manatus latirostris phospholipase C, delta 3 (LOC101344460), mRNA.

Price & Availability↑

CloneID	OTr170874
Clone ID Related Accession (Same CDS sequence)	XM_004374276.2 , XM_004374276.1
Accession Version	XM_004374276.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443964.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCG GCCGCTGGAG GAGCTGTCGC CGCCAGCCCG AGGAGCCCCC GGTGTCGGCC 
CACGTCGCGG CGCCCGTCGC GCTGCCGCCG CAGCCCGCGC CCACGGACGG CGGCATCAAG 
AGGCCCGGGC TGCGGGCGCT GAAAAAGATG GGCCTGACGG AGGACGAGGA CGTGCGCGCC 
ATGCTGCGGG GCTCGCGGCT CCGCAAGATC CGCTCGCGGA CGTGGCAGAA GGAGCGGCTG 
TACCGGCTGC AGGAGGACTG CCTGAGCGTG TGGTTCCAGC GGCGCATCCC GCGGGCGCCG 
TCGCAGCACA TCTTCTACGT GCAACACATC GAGGCCGTCC GCGAGGGCCA CCAGTCCGAA 
GGCCTGCAGC GCTTCGGCAG CGCCTTCGCG CCCGCACGCT GCCTCACCAT CGCCTTCAAG 
GGCCGCCGCA AGAACCTGGA CCTGGTGGCG CCCACGGCGG AAGAGGCGCA GCGCTGGGTG 
CGCGGCCTGG CCAAGCTGCG CGCACGCCTC GACGCCATGA GCCAGAGGGA GCGGCTCGAC 
CATTGGATTC ACTTCTACCT GCACCGGGCA GACTCCAACC AGGATAGTAA GATGAGCTTC 
AAGGAGATCA AGAGCCTGCT CAGAATGGTC AATGTGGACA TGAATGACAT GTATGCCTAC 
CACCTCTTCA AGGAGTGCGA CCACTCCAAC AACGAGAAGC TGGAAGGGGC TGAGATAGAG 
GAATTTCTGC GGCGGCTGCT GAAACGCCCT GAGTTGGAGG AGATCTTCCA TCGGTACTCG 
GGCGAGGACC ACGTGCTGAG CGCCCCTGAA CTGCTGGAGT TCCTGGAGGA CCAGGGTGAG 
GACGATGCCA CACTGACCCA CGCCCAGCAG CTCATCCAGA CCTATGAGCT CAACGAGACA 
GCCAAACAGC ATGAGCTGAT GACACTGGAT GGCTTCATGA TGTACCTGCT GTCGCCTGAA 
GGGGCTGCCC TGGACCCGGC CCACACACGC GTGTTCCAGG ACATGGACCA ACCCCTAACC 
CACTACTTCA TCTCCTCCTC CCACAATACC TACCTGACCG ACTCTCAGAT CGGGGGGCCT 
AGCAGCACCG AGGCCTATGT TAGGGCCTTT GCCCAGGGAT GCCGCTGTGT GGAGCTAGAC 
TGCTGGGAGG GCCCAGGAGG GGAACCTGTC ATCTACCATG GCCATACACT TACCTCCAAG 
ATCCTTTTCC GCGACGTGGT CCAAGCTGTG CGTGACCATG CCTTCATGCT CTCCCCATAC 
CCTGTCATCC TGTCCCTCGA GAACCACTGT GGGCTGGAGC AGCAAGCTGC CATGGCCCGC 
CACCTCCGCA CCATCCTGGG GGACATGCTG GTGACACAGG CACTGGACTC CCAGAGCCCT 
GAGGCAGTAC CATCTCCAGA GCAACTGAAG GGCCGTGTCC TGGTGAAGGG GAAGAAGCTG 
CCGGCTGCTC GGAGCGAGGA TGGCCGAATT TTACCGGACA GGGAGGAGGA GGAGGAGGAA 
GAAGAGGAAG AGGAAAAAGA GGAGGCAGAG GGTGCAGAGC AGAAGCGGAG GGCCAAGCAG 
ATCTCCCCAG AGCTGTCAGC CCTGGCTGTG TACTGCTGCG CTACCCGCCT GCAGACCCTG 
CACCTGGCCT CTGTGCCAGC CCAGCCCTGC CAGGTCAGCT CCCTCAGCGA GCGTAAGGCT 
AAGAAGCTCA TCCGAGAGGC AGGGAACAGC TTTGTCAGGC ACAATGCTCG GCAGCTGACC 
CGCGTCTACC CGCTGGGGCT TCGGATGAAC TCGGCCAATT ACAGCCCTCA GGAGATGTGG 
AACTCAGGCT GCCAGCTGGT GGCCCTGAAT TTCCAGACGC CCGGCTATGA GATGGACCTC 
AATGCTGGGC GCTTCCTCAT CAACGGGCAG TGTGGATATG TCCTGAAACC TGACTGCCTA 
CGGCAACCCG ACACAACCTT TGACCCTGAG TGCCCTAGGC CCCCCAGGAC CACACTTACC 
ATCCAGGTGC TGACTGCACA GCAGCTGCCC AAGCTGAACG CCGAGAAGCC CAACTCCATC 
GTGGACCCCC TGGTACGCAT CGAGATCCAC GGGGTGCCGG CAGACTGTGC ACGCAAGGAG 
ACTGACTACG TGCTCAACAA TGGCTTCAAT CCACATTGGG GGCAGACCCT GCAGTTCCAG 
TTGCGGGCTC CGGAGCTGGT GCTGGTCCGG TTCGTGGTCG AGGACTATGA CACCACCTCC 
CCCAATGACT TTGTGGGCCA GTTTACACTG CCTCTGAACA GCCTGAAGCA AGGGTACCGC 
CACATCCACC TGCTTTCCAA GGATGGGGCC TCACTCTCAC CAGCCACGCT CTTCGTCCAC 
ATCCGGATCC AGCACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004374333.1
CDS	95..2455
Translation	MLCGRWRSCRRQPEEPPVSAHVAAPVALPPQPAPTDGGIKRPGLRALKKMGLTEDEDVRAMLRGSRLRKIRSRTWQKERLYRLQEDCLSVWFQRRIPRAPSQHIFYVQHIEAVREGHQSEGLQRFGSAFAPARCLTIAFKGRRKNLDLVAPTAEEAQRWVRGLAKLRARLDAMSQRERLDHWIHFYLHRADSNQDSKMSFKEIKSLLRMVNVDMNDMYAYHLFKECDHSNNEKLEGAEIEEFLRRLLKRPELEEIFHRYSGEDHVLSAPELLEFLEDQGEDDATLTHAQQLIQTYELNETAKQHELMTLDGFMMYLLSPEGAALDPAHTRVFQDMDQPLTHYFISSSHNTYLTDSQIGGPSSTEAYVRAFAQGCRCVELDCWEGPGGEPVIYHGHTLTSKILFRDVVQAVRDHAFMLSPYPVILSLENHCGLEQQAAMARHLRTILGDMLVTQALDSQSPEAVPSPEQLKGRVLVKGKKLPAARSEDGRILPDREEEEEEEEEEEKEEAEGAEQKRRAKQISPELSALAVYCCATRLQTLHLASVPAQPCQVSSLSERKAKKLIREAGNSFVRHNARQLTRVYPLGLRMNSANYSPQEMWNSGCQLVALNFQTPGYEMDLNAGRFLINGQCGYVLKPDCLRQPDTTFDPECPRPPRTTLTIQVLTAQQLPKLNAEKPNSIVDPLVRIEIHGVPADCARKETDYVLNNGFNPHWGQTLQFQLRAPELVLVRFVVEDYDTTSPNDFVGQFTLPLNSLKQGYRHIHLLSKDGASLSPATLFVHIRIQHC

Target ORF information:

RefSeq Version	XM_004374276.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris phospholipase C, delta 3 (LOC101344460), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004374276.1

ORF Insert Sequence:

ATGCTGTGCG GCCGCTGGAG GAGCTGTCGC CGCCAGCCCG AGGAGCCCCC GGTGTCGGCC 
CACGTCGCGG CGCCCGTCGC GCTGCCGCCG CAGCCCGCGC CCACGGACGG CGGCATCAAG 
AGGCCCGGGC TGCGGGCGCT GAAAAAGATG GGCCTGACGG AGGACGAGGA CGTGCGCGCC 
ATGCTGCGGG GCTCGCGGCT CCGCAAGATC CGCTCGCGGA CGTGGCAGAA GGAGCGGCTG 
TACCGGCTGC AGGAGGACTG CCTGAGCGTG TGGTTCCAGC GGCGCATCCC GCGGGCGCCG 
TCGCAGCACA TCTTCTACGT GCAACACATC GAGGCCGTCC GCGAGGGCCA CCAGTCCGAA 
GGCCTGCAGC GCTTCGGCAG CGCCTTCGCG CCCGCACGCT GCCTCACCAT CGCCTTCAAG 
GGCCGCCGCA AGAACCTGGA CCTGGTGGCG CCCACGGCGG AAGAGGCGCA GCGCTGGGTG 
CGCGGCCTGG CCAAGCTGCG CGCACGCCTC GACGCCATGA GCCAGAGGGA GCGGCTCGAC 
CATTGGATTC ACTTCTACCT GCACCGGGCA GACTCCAACC AGGATAGTAA GATGAGCTTC 
AAGGAGATCA AGAGCCTGCT CAGAATGGTC AATGTGGACA TGAATGACAT GTATGCCTAC 
CACCTCTTCA AGGAGTGCGA CCACTCCAAC AACGAGAAGC TGGAAGGGGC TGAGATAGAG 
GAATTTCTGC GGCGGCTGCT GAAACGCCCT GAGTTGGAGG AGATCTTCCA TCGGTACTCG 
GGCGAGGACC ACGTGCTGAG CGCCCCTGAA CTGCTGGAGT TCCTGGAGGA CCAGGGTGAG 
GACGATGCCA CACTGACCCA CGCCCAGCAG CTCATCCAGA CCTATGAGCT CAACGAGACA 
GCCAAACAGC ATGAGCTGAT GACACTGGAT GGCTTCATGA TGTACCTGCT GTCGCCTGAA 
GGGGCTGCCC TGGACCCGGC CCACACACGC GTGTTCCAGG ACATGGACCA ACCCCTAACC 
CACTACTTCA TCTCCTCCTC CCACAATACC TACCTGACCG ACTCTCAGAT CGGGGGGCCT 
AGCAGCACCG AGGCCTATGT TAGGGCCTTT GCCCAGGGAT GCCGCTGTGT GGAGCTAGAC 
TGCTGGGAGG GCCCAGGAGG GGAACCTGTC ATCTACCATG GCCATACACT TACCTCCAAG 
ATCCTTTTCC GCGACGTGGT CCAAGCTGTG CGTGACCATG CCTTCATGCT CTCCCCATAC 
CCTGTCATCC TGTCCCTCGA GAACCACTGT GGGCTGGAGC AGCAAGCTGC CATGGCCCGC 
CACCTCCGCA CCATCCTGGG GGACATGCTG GTGACACAGG CACTGGACTC CCAGAGCCCT 
GAGGCAGTAC CATCTCCAGA GCAACTGAAG GGCCGTGTCC TGGTGAAGGG GAAGAAGCTG 
CCGGCTGCTC GGAGCGAGGA TGGCCGAATT TTACCGGACA GGGAGGAGGA GGAGGAGGAA 
GAAGAGGAAG AGGAAAAAGA GGAGGCAGAG GGTGCAGAGC AGAAGCGGAG GGCCAAGCAG 
ATCTCCCCAG AGCTGTCAGC CCTGGCTGTG TACTGCTGCG CTACCCGCCT GCAGACCCTG 
CACCTGGCCT CTGTGCCAGC CCAGCCCTGC CAGGTCAGCT CCCTCAGCGA GCGTAAGGCT 
AAGAAGCTCA TCCGAGAGGC AGGGAACAGC TTTGTCAGGC ACAATGCTCG GCAGCTGACC 
CGCGTCTACC CGCTGGGGCT TCGGATGAAC TCGGCCAATT ACAGCCCTCA GGAGATGTGG 
AACTCAGGCT GCCAGCTGGT GGCCCTGAAT TTCCAGACGC CCGGCTATGA GATGGACCTC 
AATGCTGGGC GCTTCCTCAT CAACGGGCAG TGTGGATATG TCCTGAAACC TGACTGCCTA 
CGGCAACCCG ACACAACCTT TGACCCTGAG TGCCCTAGGC CCCCCAGGAC CACACTTACC 
ATCCAGGTGC TGACTGCACA GCAGCTGCCC AAGCTGAACG CCGAGAAGCC CAACTCCATC 
GTGGACCCCC TGGTACGCAT CGAGATCCAC GGGGTGCCGG CAGACTGTGC ACGCAAGGAG 
ACTGACTACG TGCTCAACAA TGGCTTCAAT CCACATTGGG GGCAGACCCT GCAGTTCCAG 
TTGCGGGCTC CGGAGCTGGT GCTGGTCCGG TTCGTGGTCG AGGACTATGA CACCACCTCC 
CCCAATGACT TTGTGGGCCA GTTTACACTG CCTCTGAACA GCCTGAAGCA AGGGTACCGC 
CACATCCACC TGCTTTCCAA GGATGGGGCC TCACTCTCAC CAGCCACGCT CTTCGTCCAC 
ATCCGGATCC AGCACTGCTG A