LOC656961 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC656961
Entrez Gene ID	656961
Full Name	TGF-beta activated kinase 1
Synonyms	Tak1
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_963454.3	XP_968547.1	mitogen-activated protein kinase kinase kinase 7

Pathways from BioSystems
Gene Ontology

The following LOC656961 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC656961 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg11730	XM_963454.3 Latest version!	Tribolium castaneum TGF-beta activated kinase 1 (LOC656961), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC656961 ( XM_963454.3 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_968547.1 Tribolium castaneum TGF-beta activated kinase 1 (LOC656961), mRNA.

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CloneID	OTg11730
Clone ID Related Accession (Same CDS sequence)	XM_963454.3
Accession Version	XM_963454.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	mitogen-activated protein kinase kinase kinase 7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450184.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 30, 2014 this sequence version replaced XM_963454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTT CGGAAAATGA GGGTAGCGTA TGTCAGTACA CCGTCGAGGA AATCAACTAC 
AATGAAATTC AGAAGCTTGA GGCAGTCGGT GAGGGCAGCT TTGGGGTCGT TTACAAGGGA 
ATCTGGCGCG ATGCGTTCGT GGCGGTCAAA AACATCGTCT CTGAGAACGA CAAAGACGCC 
TTTATTGTGG AAGTCCGGCA GCTGTCAAGG GTCAAGCATG AGAACATTGT CAAGCTGTAC 
GGGGCCTGCA CCAAGGGCCC CAACATCTGC CTTGTGATGG AGTACGCCGA AGGGGGCTCC 
CTCTTCAATA TTCTACACCA CGCACCTAAC GTACACTACA CGATGGCCAA CGCCATGTCC 
TGGCTCTACC AGTGCGCCAA AGGGGTCGAG TATCTCCATG GCATGAAACC CAAACCTTTG 
ATACACAGAG ACCTCAAACC GCCCAACTTG CTCCTGATAA ACGGGGGCAC CTTGCTAAAG 
ATATGCGACT TTGGTACTGC CACAGACATG ACAGCAATCA TGACCAACAA CAAAGGCTCG 
GCCGCGTGGA TGGCCCCCGA GGTCTTTGAA ACGTCAAACT ATACGGAAAA GTGCGACGTC 
TTCAGCTGGG GCATAATCCT CTGGGAGGTG TTATCGCGAC TTAAGCCTTT TTTCCACATT 
AAGGAGTCGG GCTACCCTTC GGCTTTCATG ATCTTGTGGG CGAAGCACAG CGGCCGGAGG 
CCCCCTCTCA TCAGGCACTG CCCCCCAGTC ATCGAAAAAC TGATGACACA GTGCTGGGAT 
CAGGAGGCGG CCAAGCGGCC GAGCATGAAG GAGATTGTGA AAAAAATGGA AGTTATTTGC 
TCGTTGCTTC CGGGCGCCGA CGAGCCGTTG CAGATCAGGG GCGGTGACGA TGTGGTTTAC 
AAGGAGGAAA TCGAAGAGTT TGAGGACTGT GTGGAGGAAA TAGACCAGTT CCCCGCCAGC 
ACCAACGGCG ATAATTGTGT CAAATCGCTG CCCCAGCCGA GTGCCGAGAT GTCGCGGCCC 
CTCAACATAA AAATAAGCCC TGACGCATGG GATTTACACG ATCTCGACAT GCAGCTGAGA 
ACCATGCCCG GCCTTGATAA GATGGTACCA AAAAACGGTT CCAAAAACAC AACGGTAGAG 
GCAGGCATCG AGAGCACCAA GTCGACACCT GACGGCACCG ATCCAAATTT AGATAAAGTG 
TATCTTTTAT TAGAGCCGCA TTTACGACCT GCCACTCCAG ACGCCGACAA TCCGCAGTCG 
TTGGCGCTGT TCGAAGAGCA CAAGCAACTG GCTCAGGAAT ATTTCAAGGT CCAAACTGAG 
CTCGCATATG TCTCGCAAAA GAAGGACAAA CTCCTAGCGG CGCAGTCGCA AGAACAGCAG 
CAGCACACCA AACTCCTCAA AGACCTGCAA GCGGAGAAGG AGTCCCTGAT CTTGGTGCGG 
GAGTTGCTCA GTCGGCAAAG AGAAAACAAC AACGCGGCTA GGAATAACAT AGATAACTGG 
GTCCTGGTGC CCAGAGATGG TACTAACACG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_968547.1
CDS	115..1650
Translation	MASSENEGSVCQYTVEEINYNEIQKLEAVGEGSFGVVYKGIWRDAFVAVKNIVSENDKDAFIVEVRQLSRVKHENIVKLYGACTKGPNICLVMEYAEGGSLFNILHHAPNVHYTMANAMSWLYQCAKGVEYLHGMKPKPLIHRDLKPPNLLLINGGTLLKICDFGTATDMTAIMTNNKGSAAWMAPEVFETSNYTEKCDVFSWGIILWEVLSRLKPFFHIKESGYPSAFMILWAKHSGRRPPLIRHCPPVIEKLMTQCWDQEAAKRPSMKEIVKKMEVICSLLPGADEPLQIRGGDDVVYKEEIEEFEDCVEEIDQFPASTNGDNCVKSLPQPSAEMSRPLNIKISPDAWDLHDLDMQLRTMPGLDKMVPKNGSKNTTVEAGIESTKSTPDGTDPNLDKVYLLLEPHLRPATPDADNPQSLALFEEHKQLAQEYFKVQTELAYVSQKKDKLLAAQSQEQQQHTKLLKDLQAEKESLILVRELLSRQRENNNAARNNIDNWVLVPRDGTNTD

Target ORF information:

RefSeq Version	XM_963454.3
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum TGF-beta activated kinase 1 (LOC656961), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_963454.3

ORF Insert Sequence:

ATGGCGTCTT CGGAAAATGA GGGTAGCGTA TGTCAGTACA CCGTCGAGGA AATCAACTAC 
AATGAAATTC AGAAGCTTGA GGCAGTCGGT GAGGGCAGCT TTGGGGTCGT TTACAAGGGA 
ATCTGGCGCG ATGCGTTCGT GGCGGTCAAA AACATCGTCT CTGAGAACGA CAAAGACGCC 
TTTATTGTGG AAGTCCGGCA GCTGTCAAGG GTCAAGCATG AGAACATTGT CAAGCTGTAC 
GGGGCCTGCA CCAAGGGCCC CAACATCTGC CTTGTGATGG AGTACGCCGA AGGGGGCTCC 
CTCTTCAATA TTCTACACCA CGCACCTAAC GTACACTACA CGATGGCCAA CGCCATGTCC 
TGGCTCTACC AGTGCGCCAA AGGGGTCGAG TATCTCCATG GCATGAAACC CAAACCTTTG 
ATACACAGAG ACCTCAAACC GCCCAACTTG CTCCTGATAA ACGGGGGCAC CTTGCTAAAG 
ATATGCGACT TTGGTACTGC CACAGACATG ACAGCAATCA TGACCAACAA CAAAGGCTCG 
GCCGCGTGGA TGGCCCCCGA GGTCTTTGAA ACGTCAAACT ATACGGAAAA GTGCGACGTC 
TTCAGCTGGG GCATAATCCT CTGGGAGGTG TTATCGCGAC TTAAGCCTTT TTTCCACATT 
AAGGAGTCGG GCTACCCTTC GGCTTTCATG ATCTTGTGGG CGAAGCACAG CGGCCGGAGG 
CCCCCTCTCA TCAGGCACTG CCCCCCAGTC ATCGAAAAAC TGATGACACA GTGCTGGGAT 
CAGGAGGCGG CCAAGCGGCC GAGCATGAAG GAGATTGTGA AAAAAATGGA AGTTATTTGC 
TCGTTGCTTC CGGGCGCCGA CGAGCCGTTG CAGATCAGGG GCGGTGACGA TGTGGTTTAC 
AAGGAGGAAA TCGAAGAGTT TGAGGACTGT GTGGAGGAAA TAGACCAGTT CCCCGCCAGC 
ACCAACGGCG ATAATTGTGT CAAATCGCTG CCCCAGCCGA GTGCCGAGAT GTCGCGGCCC 
CTCAACATAA AAATAAGCCC TGACGCATGG GATTTACACG ATCTCGACAT GCAGCTGAGA 
ACCATGCCCG GCCTTGATAA GATGGTACCA AAAAACGGTT CCAAAAACAC AACGGTAGAG 
GCAGGCATCG AGAGCACCAA GTCGACACCT GACGGCACCG ATCCAAATTT AGATAAAGTG 
TATCTTTTAT TAGAGCCGCA TTTACGACCT GCCACTCCAG ACGCCGACAA TCCGCAGTCG 
TTGGCGCTGT TCGAAGAGCA CAAGCAACTG GCTCAGGAAT ATTTCAAGGT CCAAACTGAG 
CTCGCATATG TCTCGCAAAA GAAGGACAAA CTCCTAGCGG CGCAGTCGCA AGAACAGCAG 
CAGCACACCA AACTCCTCAA AGACCTGCAA GCGGAGAAGG AGTCCCTGAT CTTGGTGCGG 
GAGTTGCTCA GTCGGCAAAG AGAAAACAAC AACGCGGCTA GGAATAACAT AGATAACTGG 
GTCCTGGTGC CCAGAGATGG TACTAACACG GACTGA